<target(TargetType;GenbankID;miRBaseAccessionNumber;Description;Sequence;Information)
miRNA		MI0000077	Homo sapiens miR-21 stemp-loop	UGUCGGGUAGCUUAUCAGACUGAUGUUGACUGUUGAAUCUCAUGGCAACACCAGUCGAUGGGCUGUCUGACA	"Mature sequence Accession:MIMAT0000076;ID:hsa-miR-21 Sequence(8-29) 5'-uagcuuaucagacugauguuga-3'"






>
<reference(ReferenceID;PubMedID;Author;Title;Journal;Book;Date;Volumn;Issue;Pages;Path)
ref000001		Scott Davis, Bridget Lollo, Susan Freier and Christine Esau	"Improved targeting of miRNA with antisense oligonucleotides"	Nucleic Acids Research		2006	34	8	2294-2304	
ref000002		Angie M. Cheng, Mike W. Byrom, Jeffrey Shelton and Lance P. Ford	Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis	Nucleic Acids Research		2005	33	4	1290-1297	


>
<antiseq(AntiID;AntiType;SeqType;Seq;Strand;Source;Postion;shRNAseq;Modification;RefIDs)
AOs000001	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1-22)2'-MOE PO	ref000001
AOs000002	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1-22)2'-MOE PS	ref000001
AOs000003	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1-12)2'-MOE PS	ref000001
AOs000004	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(11-22)2'-MOE PS	ref000001
AOs000005	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(6-17)2'-MOE PS	ref000001
AOs000006	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1-22)2'-OMe PS	ref000001
AOs000007	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1-22)2'-OMe PO	ref000001
AOs000008	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1-5,16-22)2'-MOE PS	ref000001
AOs000009	AOs	DNA	TCAACATCAGTCTGATAAGCTA	-	synthenticOligo	Fs|8-29		(1,3,5,7,9,11,13,15,17,19,21,22)2'-MOE PS	ref000001
AOs000010	AOs	RNA	ucaacacagucugauaagcua	-	synthenticOligo	Fs|8-29		(1-21)2'-OMe	ref000002


>
<antiassay:InVitro:sid200634:ref000001:Lipofectin
{efficacy(AntiID;RefID;CtrlIDs;CellLine;AssayLevel;AssayMethod;Conc;Efficacy)
AOs000001	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	++++
AOs000002	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	+++
AOs000003	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	+++
AOs000004	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	+
AOs000005	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	+
AOs000006	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	+
AOs000007	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	+
AOs000008	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	-
AOs000009	ref000001	ctr000001	Hela	miRNA	luciferase reporter assay	33 nM	-

}
{control(CtrlID;CtrlType;CtrlSeq;AssayLevel;AssayMethod;Conc;Eff)
ctr000001	NC	Unrated sequence	miRNA	luciferase reporter assay	33 nM	

}
{figure(FigID;FigName;FigURL;FigPath;AntiIDs;CtrlIDs;Title;Annotation)

}
{comments()
dididd
}
>
<antiassay:InVitro:sid200533:ref000002:lipofectamine 2000
{efficacy(AntiID;RefID;CtrlIDs;CellLine;AssayLevel;AssayMethod;Conc;Efficacy)
AOs000010	ref000002	ctr000002	Hela	miRNA	miRNA array	10-30 pmol 	+++
AOs000010	ref000002	ctr000002	A549	miRNA	miRNA array	10-30 pmol	++++

}
{control(CtrlID;CtrlType;CtrlSeq;AssayLevel;AssayMethod;Conc;Eff)
ctr000002	NC	guggauauuguugccauca	miRNA	miRNA array	10-30 pmol 	

}
{figure(FigID;FigName;FigURL;FigPath;AntiIDs;CtrlIDs;Title;Annotation)

}
{comments()

}
>