All you need to do is enter your primer's names, their sequences, and their sense (F for forward or sense, R for reverse or antisense). Then, click the multiplex button. Answer the questions as they pop up, and that's all there is. If you want to run the macro again to get more 3' complimentarity graphs, you may. However, you can only run the macro once for each possibility. Also, if you re-run the macro, do not ask it to calculate melting temps again, as this will cause it to crash. The reason is that Visual Basic will run into a problem in trying to name a new sheet with the same name as a sheet that already exists. You should have all the information you need after running the Melt part of the program once.
Description of the macro.
Download the macro.
Back to the program list.
This page was last updated on: August 28, 2002 by David G. Cox