[BiO BB] Primer BLAST

Bill_A_Nussbaumer at bd.com Bill_A_Nussbaumer at bd.com
Thu Nov 21 14:08:39 EST 2002

Hi all,

I'm hoping a method exists that would allow me to align PCR primers against
the human genome to determine all products that could be amplified if my
sample contained extracted human DNA.  Theoretically I could BLAST each
primer with stringent parameters and record the coordinates on the genome
looking for matches with a Kb or two of one another.  To do that manually
would be quite tedious, especially when you consider that I'm only really
interested in matches at the 3' end.

Anyone know of a way to do this?

- Bill

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