[BiO BB] Problem with BLAST
Jose Maria Gonzalez Izarzugaza
biopctgi at yahoo.es
Tue Jun 14 10:57:43 EDT 2005
The fact is that BLAST counts the percentage of [ATCG] in the query or
something similar.
You can try a query like that ATATACACATTTGArrrrrrr setting the option
'Mask lower case' to active.
It works (or seems to) and returns an aligment for the ATATAT...GA
zone... by chance???... Maybe. :o(
Good luck!
Txema
Joe Landman wrote:
> Did you mean to translate your nucleotide sequence into a protein
> sequence? You would need to use either blastx to do the translation,
> or one of the tblast series (depending upon what you were searching
> against).
>
> Balaji wrote:
>
>> Hi everyone,
>> I have a protein that comprises only of Alanine(A), Glycine(G),
>> Cysteine(C) and Threonine(T). When i try to use NCBI BLAST with this
>> protein, the blast program returns an error saying that the entered
>> sequence is a nucleotide (as it evidently has only A's, T's, C's and
>> G's) and not a protein sequence.
>> Is there anyway i work around the problem? I'll be grateful for
>> any help
>> Thanks in advance
>> Balaji
>
>
>
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