[BiO BB] Spooling DNA

Martin Gollery marty.gollery at gmail.com
Tue Oct 25 23:36:36 EDT 2005

Would I want to take a MolBio 1 week course? Definitely. Would I ever likely
have the time to do so? Almost certainly not.

The second problem with putting something like that on is that
bioinformaticians are widely varied in background and daily tasks, so coming
up with something that is relevant to each would not be easy.


On 10/25/05, Dale Beach <dbeach at email.unc.edu> wrote:
> You can spool the DNA because of its length. RNA and proteins are both
> easily precipitated but need a centrifuge to rapidly collect the
> precipitate. As for the DNA precipitation from an onion, assuming that you
> are using an alcohol (isopropanol or ethanol are common) you should also be
> precipitating the RNA since the chemical properties of DNA and RNA are
> similar (they are both nucleotide chains!). The RNA just doesn't spool like
> the DNA. And even though there are plenty of RNAses (along with proteases
> and DNAses), the RNA will still precipitate with the DNA though it might be
> a little fragmented!
> The real question is how many folks reading this have actually done any
> wet-lab molecular biology? I come from a MolBio background, and wonder how
> many bioinformaticians have gotten their hands dirty in a MolBio lab? More
> importantly how many would LIKE TO? If there were a course available, say 1
> week where you cloned and sequenced a gene then expressed a protein, would
> you take it? What else would you want to do? The goal would be to provide
> some practical experience with the molecules that so many folks are busy
> modeling.
> dale
> Dale Beach, PhD
> SPIRE Postdoctoral Fellow, UNC-CH
> Duke University Medical Center
> Jones CB3020
> Durham, NC 27710
> Laura Nielson wrote:
> Why is it not possible to spool out precipitated proteins in comparison to
> spooling out precipitated DNA? DNA is very, very long. How long are proteins
> (polypeptides)?
>  I've done the experiment where you precipitate DNA out of onion cells.
> Could you precipitate RNA out of onion cells? How? or Why not?
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Martin Gollery
Associate Director
Center For Bioinformatics
University of Nevada at Reno
Dept. of Biochemistry / MS330
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