[BiO BB] How to find the same proteins?

Pankaj pankaj at nii.res.in
Thu Mar 23 05:15:18 EST 2006


For this u can go to NCBI BLAST page and go to BLASTP. There u can paste ur
sequence and select PDB as the database to query. Just click on BLAST and u
get all seq similar to ur sequence. Filter out the results to find PDB ids
>99% similar to ur protein.
Sine u have 200 proteins u can download NCBI database and run local BLAST also.
Cheers 
Pankaj Khurana
Research Scholar
National Institute of Immunology
New Delhi
India
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---------- Original Message -----------
From: Semen Esilevsky <yesint4 at yahoo.com>
To: bio_bulletin_board at bioinformatics.org
Sent: Thu, 23 Mar 2006 01:30:58 -0800 (PST)
Subject: [BiO BB] How to find the same proteins?

> Dear all,
> I'm a novice in bioinformatics and this question is
> probably stupid, but...
> I have a list of ~200 PDB id's. For each of them I
> have to build a list of all entries in PDB, which
> represent the same protein (say, >99% sequence
> similarity and no large gaps). Could someone suggest
> me the least painfull way of doing this?
> As far as I understand all what I need is the database
> where all pairwice BLAST allignments of PDB chains are
> stored. I've found one as a part of a PISCES server,
> but it is incomplete and contains some internal
> inconsistensies. Could someone suggest me a better one
> or there is a simpler way out?
> 
> Best,
> Semen
> 
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------- End of Original Message -------




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