[BiO BB] RefSeq mRNA sequences of barley

[Ethan Strauss]

Hi,
	I think that there are two problems. The first is that there do
not seem to be any barley sequences in refseq (with the exception of the
chloroplast genome NC_008590), the second may be that you need to use
the scientific name for the organism ("Hordeum vulgare"). I have had
variable luck using common names as [organism]. 
	I don't have any clue why there are no barley records in
RefSeq...
Hope this helps a little. 
Ethan
Ethan Strauss Ph.D.
Bioinformatics Scientist
Promega Corporation
2800 Woods Hollow Rd.
Madison, WI 53711
608-274-4330
800-356-9526
ethan.strauss at promega.com

-----Original Message-----
From:
bio_bulletin_board-bounces+ethan.strauss=promega.com at bioinformatics.org
[mailto:bio_bulletin_board-bounces+ethan.strauss=promega.com at bioinformat
ics.org] On Behalf Of George Tzanis
Sent: Saturday, July 21, 2007 3:39 PM
To: 'General Forum at Bioinformatics.Org'
Subject: [BiO BB] RefSeq mRNA sequences of barley

Dear All,
 
I would like to get all the non-redundant cDNA sequences of barley.
For this reason I'm thinking about retrieving all RefSeq mRNAs of
barley. I have made a search to the Core Nucleotide database
(http://www.ncbi.nlm.nih.gov/sites/entrez?db=nuccore) using the
following query:
 
"barley[organism] AND biomol_mRNA[properties] AND srcdb_refseq[PROP]"
 
However, not any sequences were returned. I have also used the Entrez
Limits settings, but the result was the same. 
 
Is there something wrong in my search?
Are there any RefSeq mRNAs for barley?
Is there another way to get a non-redundant set of cDNA (or mRNA)
sequences of barley? I will appreciate any idea.
 
Thank you in advance,
 
George Tzanis
Department of Informatics
Aristotle University of Thessaloniki
54124 Thessaloniki
Greece
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