[BiO BB] Newbie question.
drjohn08318 at yahoo.com
drjohn08318 at yahoo.com
Sun Jan 6 19:20:30 EST 2008
Hi:
Why not express the protein in E. coli, then carry out EMSA usong the recombinant protein to locate the binding sites on the DNA.
John
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-----Original Message-----
From: "Donnie Darko" <rabidstrikes at googlemail.com>
Date: Sun, 6 Jan 2008 14:57:00
To:bbb at bioinformatics.org
Subject: [BiO BB] Newbie question.
Hi folks,
Suppose I have a novel gene whose product is a DNA binding protein, how
would I go about locating potential binding sites where this protein could
bind? I'm only interested in finding sites for this specific protein and not
a general scan of tfbs.
I've extracted upstream sequences of genes that I'm interested in but I
can't think of a way forward.
Cheers and belated happy new year :-P
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