Caveat: still a complete newb, please forgive my ignorance - I tried to find similar problems but I am not doubt looking in the wrong place. RTFM replies are welcome, if they include a link to TFM :) We're experiencing a problem where our BLAST nt db is > 2 GB and this causing problems for our linux STDIN/STDOUT. Does this problem exist in Biobrew? If it does, can biobrew work around it by splitting up the db and recombining the results? Cheers, Chris -- Chris Beck / Y.A.B.A. / Fungal Genomics / CFSG / Concordia University Chaos. Panic. Confusion. My work here is done.