Hi Glen, Thanks for the rpms. The new lam is working fine as we can run some of the programs with this lam. But the problem starts when we try to run mpiblast from your ncbi rpm. It asks for lamb to run. We tried with the following command: /opt/lam/7.0.4/bin/mpirun n0 N /opt/BioBrew/NCBI/6.1.0/bin/mpiblast --conf-file=/opt/BioBrew/NCBI/6.1.0/etc/mpiblast.conf -p blastp -i mpi_test_blast/test.seq -d swissprot -o results.txt and the output is as bellow: ----------------------------------------------------------------------------- It seems that there is no lamd running on the host . This indicates that the LAM/MPI runtime environment is not operating. The LAM/MPI runtime environment is necessary for MPI programs to run (the MPI program tired to invoke the "MPI_Init" function). Please run the "lamboot" command the start the LAM/MPI runtime environment. See the LAM/MPI documentation for how to invoke "lamboot" across multiple machines. ----------------------------------------------------------------------------- ----------------------------------------------------------------------------- It seems that [at least] one of the processes that was started with mpirun did not invoke MPI_INIT before quitting (it is possible that more than one process did not invoke MPI_INIT -- mpirun was only notified of the first one, which was on node n0). mpirun can *only* be used with MPI programs (i.e., programs that invoke MPI_INIT and MPI_FINALIZE). You can use the "lamexec" program to run non-MPI programs over the lambooted nodes. ----------------------------------------------------------------------------- Moreover, while installing ncbitools-6.1.0-1.i386.rpm it showed the following warning: user glen does not exist - using root Is the ncbitools-6.1.0-1.i386.rpm is not proper or we are making some mistake while running the mpiblast. with regards, Soumyadeep __________________________________ Do you Yahoo!? Yahoo! Small Business $15K Web Design Giveaway http://promotions.yahoo.com/design_giveaway/