[BioBrew Users] FW: Re: [Bioclusters] re: how to run mpiblast

[kenix y]

mpiBLAST is ready to run in BioBrew.

1)I had set up .ncbirc

[NCBI]
Data=/opt/BioBrew/NCBI/6.1.0/data
[BLAST]
BLASTDB=/tmp/blast

2) and mpiblast.conf

/mnt/pvfs
/tmp/blast
/opt/BioBrew/NCBI/6.1.0/bin

3) edit my /etc/profile
>export BLASTPATH=/opt/BioBrew/NCBI/6.1.0/bin
>export MPIBLASTCONFIG=/home/build/mpiblast.conf
>export MPIRUN=/opt/mpich-vmi-2.0-gcc/bin/mpirun

4) my $HOME/machines contains
comp-pvfs-0-0

But this command gave me the mpirun helpfile
$MPIRUN -np 2 -machinefile $HOME/machines $BLASTPATH/mpiblast
config-file=$MPIBLASTCONFIG -p blastn -d ecoli.nt -i test.fas -o blast.txt

and this command
mpirun -np 2 -machinefile $HOME/machines $BLASTPATH/mpiblast
config-file=$MPIBLASTCONFIG -p blastn -d ecoli.nt -i /tmp/blast/test.fas -o 
/tmp/blast/blast_results.txt

gave me
mv: cannot stat '/root/blast/ecoli.ntChNsWp': No such file or directory
cp: cannot create regular file '/root/blast/ecoli.nt.00.nhr': Permission 
denied
cp: cannot create regular file '/root/blast/ecoli.nt.00.nin': Permission 
denied
cp: cannot create regular file '/root/blast/ecoli.nt.00.nsq': Permission 
denied
cp: cannot create regular file '/root/blast/ecoli.nt.00.nnd': Permission 
denied
cp: cannot create regular file '/root/blast/ecoli.nt.00.nni': Permission 
denied
cp: cannot create regular file '/root/blast/ecoli.nt.00.nsd': Permission 
denied
cp: cannot create regular file '/root/blast/ecoli.nt.00.nsi': Permission 
denied
Error opening /root/blast/ecoli.ntChNsWp.nal

Can anyone tell if it's my configuration or was it my command that's wrong?

Thank you

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