Regarding 3) edit my /etc/profile export BLASTPATH=/opt/BioBrew/NCBI/6.1.0/bin export MPIBLASTCONFIG=/home/bigcpuguest/mpiblast.conf export MPIRUN=/usr/bin/mpirun This is the correct one, forgot to edit the mail just now, sorry. I've move ecoli.nt to /home/bigcpuguest and chmod 777 it, but when I mpiformatdb, it always gives me this: Moving fragments to /mnt/pvfs mv: cannot stat '/home/bigcpuguest/ecoli.nt.nal': No such file or directory Created and moved 3 fragments. After I do that, there'll be a (null).nal in my /home/bigcpuguest. Is there anything to do with my mpiblast not being able to run? The output is still the same as my previous mail. _________________________________________________________________ Get 10mb of inbox space with MSN Hotmail Extra Storage http://join.msn.com/?pgmarket=en-sg