Hi Sylvain, I've found and testing seqsplit (and blastunsplit) that you can download form here ftp://ftp.cgr.ki.se/pub/prog/MSPcrunch+Blixem/ Here is the web documentation: http://www.cgr.ki.se/cgr/groups/sonnhammer/MSPcrunch.html Unfortunately seems it works only with a single-sequence at time, it means that you cannot submit multi-sequences querys, but you can modify yourself the source code. I would like to do it, so if you modify it before me let me know! Mario > Message: 2 > Date: Fri, 23 Aug 2002 14:51:14 -0400 > From: Sylvain Foisy <sylvain.foisy@bioneq.qc.ca> > To: bioclusters@bioinformatics.org > Subject: [Bioclusters] Re: Help on BLAST > Reply-To: bioclusters@bioinformatics.org > > Hi > > On Friday, August 23, 2002, at 12:01 PM, bioclusters- > request@bioinformatics.org wrote: > > > I read your posts saying "splitting the query sequence into small = > > fragments and BLASTing each of those fragments against the (entire) = > > database is super-easy to implement." Could you please tell me how to = > > combine the results, or a link to the solution would be very helpful? > > Add me to the list of interested parties to that subject. I would like > to know how to write an app that would do these three steps: > > -Splitting a sequence in multiples of, let say, 100 nucleotides; > -Send each of them to a node for BLASTing; > -Reassemble the different results into a single report for the users. > > Any web links that would help us in our quest? > > Cordially > > Sylvain > > ++++++++++++++++++++++++++++++++++++++++++++++++++++++++ > Sylvain Foisy, Ph. D. > Directeur-Operations / Project Manager > BioNEQ - Le Reseau quebecois de bioinformatique > Genome-Quebec > Tel.: (514) 878-9911 > E-mail: sylvain.foisy@bioneq.qc.ca > ++++++++++++++++++++++++++++++++++++++++++++++++++++++++ -- Dr. Mario Belluardo Institute for Cancer Research and Treatment http://www.ircc.it