Hi All, I can able to run blastall when the BLOSUM62 file is in the local directory and I am getting the results, if I try to run from some other location and giving the path to the BLOSUM62 file it is giving the following error! Command: ------- #blastall -p blastp -d /usr/local/db/pataa -i /usr/local/sge/QUERY -M /usr/local/db/BLOSUM62 Error: ----- [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: BLOSUM80 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: BLOSUM62 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: BLOSUM50 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: BLOSUM45 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: PAM250 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: BLOSUM62_20 is a supported matrix[blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: BLOSUM90 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: PAM30 is a supported matrix [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: BlastKarlinBlkGappedCalc: PAM70 is a supported matrix --------------- In that error it is searching BLOSUM62 also, help me solve this error. Thanks, Bala. __________________________________________________ Do you Yahoo!? Faith Hill - Exclusive Performances, Videos & More http://faith.yahoo.com