Hi, I'm a cluster admin and I try to help my biologists on their desktops as well. I've got a few people who have been using Bioedit, but it is unmaintained and does not work on their newer machines running MS Windows XP. I've searched a bit and found that others are also having trouble with it, especially with clustalw. I'm pointing my biologists at the Staden package and ClustalX as possible/partial? replacements for Bioedit, but I'm pretty sure that they aren't a complete replacement. Here is a list of the things Bioedit does that they want, and I would appreciate any help in locating applications that would fit. cutting and pasting sequences aligning multiple sequences in the same window (clustalW) similarity matrix slide residues grab and drag inserting/deleting gaps nucleotide composition complement and reverse compliment restriction map create concensus sequence phylogenetic tree find conserved regions identity/similarity shading of nucleotides color manipulation BLAST over the internet (allows BLASTing of multiple sequences) Some of these features, such as the integrated BLAST network client aren't as critical since I've set up a local server. I'm also setting up Phred/Phrap, Artemis, and ClustalW on the server, but I know they will continue to want something like Bioedit on their desktops. So if anyone has any suggestions I would appreciate them. Thank you, Andrew -- Andrew Shewmaker, Associate Engineer Phone: 1-208-526-1276 Idaho National Eng. and Environmental Lab. P.0. Box 1625, M.S. 3605 Idaho Falls, Idaho 83415-3605