I'm looking to put together a small cluster for a very large number of blasts against a local copy of the the ncbi nr database. I am under the impression the best thing I can do is to get it all in memory, for which I'm estimating 24GB should cover me. I've found a vendor for 3 dual 2.4G Xenon each with 8GB (ram: DDR 8x1G ecc pc2100). The total price is about $12k for the 3 of them. would mpiblast benefit from a 64 bit platform, or will 32 bits perform reasonably close. I hope to just add 1 more machine in the future as nr grows. Given this config what might be my bottleneck? Any advice, experience, or warnings would be greatly appreciated. ===== Mike Cariaso