[Bioclusters] [mpiBLAST]how to "mpiformatdb nr"~~?THX~

Osborne, John bioclusters@bioinformatics.org
Tue, 13 May 2003 16:21:12 -0400


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I'm not sure why you don't have an nr.dbs, I just started installing
mpiblast (taking most of the day so far)
and I did get an nr.dbs in my shared storage directory.
 
I have other problems though, for instance I get a 3 line (null).pal in my
shared storage directory instead of the
nr.pal it is looking for.   Anyone know what happened with this?
 
In addition, when I run mpiformatdb and tell it to break it into 20 chunks
it creates 21, from 0 to 20.
Pretty annoying.
 
The other problem I ran into when I was running mpiformatdb (on nr, is there
preformatted stuff I can
 download now?) is that it took really long.  Sure enough, it was only
running on one machine and when I
peaked at the mpiformatdb.cpp I noticed there were no MPI_ function
calls...?!  No big deal, but I find the
name of the program somewhat misleading.
 
Anybody solve these problems?  I'm using 1.1.0. I actually tried using an
earlier version of mpiblast before but I couldn't get it to
compile.  Still seems more promising than the non-MPI query splitting
variant I have been using in the past since each mini
database should be able to fit into RAM on our nodes.
 
 -John
 

-----Original Message-----
From: kwLee [mailto:g912803@student.thu.edu.tw]
Sent: Monday, May 12, 2003 10:32 PM
To: bioclusters@bioinformatics.org
Subject: [Bioclusters] [mpiBLAST]how to "mpiformatdb nr"~~?THX~


i think i have installed mpiblast already
i can use "mpiformatdb" to format yeast.nt database into same fragment
then use "mpiblast blastn" to search sequence
so i try "nr" database
i have formated nr database(use mpiformat)
but when i use "mpiblast blastp" to search
it says that "ERROR opening...nr.dbs"
i don't know why it has no nr.dbs
THX~
 


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<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>I'm 
not sure why you don't have an nr.dbs, I just started installing mpiblast 
(taking most of the day so far)</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>and I 
did get an nr.dbs in my shared storage directory.</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2></FONT></SPAN>&nbsp;</DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>I have 
other problems though, for instance I get a 3 line (null).pal in my shared 
storage directory instead of the</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>nr.pal 
it is looking for.&nbsp;&nbsp; Anyone know what happened with 
this?</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2></FONT></SPAN>&nbsp;</DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>In 
addition, when I run mpiformatdb and tell it to break it into 20 chunks it 
creates 21, from 0 to 20.</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>Pretty 
annoying.</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2></FONT></SPAN>&nbsp;</DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>The 
other problem I ran into when I was running mpiformatdb (on nr, is there 
preformatted stuff I can</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2>&nbsp;download now?) is that it took really long.&nbsp; Sure enough, it 
was only running on one machine and when I</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>peaked 
at the&nbsp;mpiformatdb.cpp I noticed there were no MPI_ function 
calls...?!&nbsp; No big deal, but I find the</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff size=2>name 
of the program somewhat misleading.</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2></FONT></SPAN>&nbsp;</DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2>Anybody solve these problems?&nbsp; I'm using 1.1.0. I actually tried 
using an earlier version of mpiblast before but I couldn't get it 
to</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2>compile.&nbsp;&nbsp;Still seems more promising than the non-MPI query 
splitting variant I have been using in the past since each 
mini</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2>database should be able to fit into RAM on our nodes.</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2></FONT></SPAN>&nbsp;</DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2>&nbsp;-John</FONT></SPAN></DIV>
<DIV><SPAN class=758550920-13052003><FONT face=Arial color=#0000ff 
size=2></FONT></SPAN>&nbsp;</DIV>
<BLOCKQUOTE dir=ltr style="MARGIN-RIGHT: 0px">
  <DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma 
  size=2>-----Original Message-----<BR><B>From:</B> kwLee 
  [mailto:g912803@student.thu.edu.tw]<BR><B>Sent:</B> Monday, May 12, 2003 10:32 
  PM<BR><B>To:</B> bioclusters@bioinformatics.org<BR><B>Subject:</B> 
  [Bioclusters] [mpiBLAST]how to "mpiformatdb nr"~~?THX~<BR><BR></FONT></DIV>
  <DIV><FONT size=2>i think i have installed mpiblast already</FONT></DIV>
  <DIV>i can use "mpiformatdb" to&nbsp;format yeast.nt database into same 
  fragment</DIV>
  <DIV>then use "mpiblast blastn" to search sequence</DIV>
  <DIV>so i try "nr" database</DIV>
  <DIV>i have formated nr database(use mpiformat)</DIV>
  <DIV>but when i use "mpiblast blastp" to search</DIV>
  <DIV>it says that "ERROR opening...nr.dbs"</DIV>
  <DIV><FONT size=2>i don't know&nbsp;why it has no nr.dbs</FONT></DIV>
  <DIV>THX~</DIV>
  <DIV>&nbsp;</DIV></BLOCKQUOTE></BODY></HTML>

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