Hi, This has been a topic before, but I am still in need of suggestions on the job that I try to do. I need to build a local Genbank human, mouse and zebrafish blast database which is updated fairly frequently if not nightly, and be able to run the btblastall from iNquiry software to parallel blast job. I could think of two ways to get the database, but am troubled with the updates on both. One is to get the nt database and run blast with gi list of the species interested. I will have to get FASTA data from NCBI so that to format it in a way that the btblastall could parallel with. But I don't think the NCBI site support rsync, ture? Then what are people's solution for frequent update? Another problem of this strategy is the gi list also has to be updated, I don't have a good idea on that either... Another choice is to parse the genbank release to get initial data, and use the daily file for updates. But as fmerge is no longer supported, is there a good way to do the merge with NCBI db format? (WU BLAST package has utility to achieve that.) Help me out! Thanks, Peiran Song Zebrafish Information Network