--0__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B Content-type: multipart/alternative; Boundary="1__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B" --1__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B Content-type: text/plain; charset=ISO-2022-JP Content-transfer-encoding: quoted-printable How much is your large genome size(MB/GB)? The assembling method is als= o decided by your genome size/repeats complexity. Using parallel phrap ju= st can solve simple and small genome assembling. Email: zengjinz@cn.ibm.com Notes: Zeng Jin Zhang/China/Contr/IBM@IBMCN = bioclusters-reque = st@bioinformatics = .org = To Sent by: bioclusters@bioinformatics.org = bioclusters-admin = cc @bioinformatics.o = rg Subj= ect Bioclusters digest, Vol 1 #552 -= 3 msgs = 2004-09-02 00:01 = = = Please respond to = bioclusters = = = Digest etiquette: When replying, PLEASE edit your Subject line so it is more specific than "Re: Bioclusters digest, Vol..." And, PLEASE quote only related text -- do not quote the entire digest. Thank you. Today's Topics: 1. Parallel assembling of a shotgun genome (=3D?iso-8859-1?Q?Octavio_Mart=3DEDnez_de_la_Vega?=3D) 2. Re: Parallel assembling of a shotgun genome (Joe Landman) 3. Re: Parallel assembling of a shotgun genome (Charles Wust) --__--__-- Message: 1 From: =3D?iso-8859-1?Q?Octavio_Mart=3DEDnez_de_la_Vega?=3D <omartine@ira.cinvestav.mx> To: <bioclusters@bioinformatics.org> Date: Tue, 31 Aug 2004 12:05:33 -0500 Subject: [Bioclusters] Parallel assembling of a shotgun genome Reply-To: bioclusters@bioinformatics.org Hi, We are a genomic research center in M=3DE9xico and we will be initiatin= g =3D the shotgun sequence of a large genome. Currently we do our bioinformatics in a 32 processor cluster, but we assemble (for example ESTs sequences)= using Phrap and/or CAP3. The question is, Can I parallelize the assembling of a shotgun sequencing project to use= the power of a cluster? Thanks in advance, Octavio. ------------------------------------ Octavio Mart=3DEDnez de la Vega Profesor Titular omartine@ira.cinvestav.mx Km. 9.6 Lib. Nte., Carretera Irapuato-Le=3DF3n, Apartado Postal 629, CP= 36500 Irapuato, Gto. M=3DE9xico. tel: (55) 462 6239649 fax: (55) 462 6239660 mobile: 044 462 4822309 ------------------------------------ --__--__-- Message: 2 Date: Tue, 31 Aug 2004 13:12:09 -0400 From: Joe Landman <landman@scalableinformatics.com> To: bioclusters@bioinformatics.org, omartine@ira.cinvestav.mx Subject: Re: [Bioclusters] Parallel assembling of a shotgun genome Reply-To: bioclusters@bioinformatics.org Hi Octavio: Have a look at SPS Phrap Suite at http://www.geospiza.com/tools/sps-phrap.htm . Joe Octavio Mart=1B$B4Z=1B(Bez de la Vega wrote: >Hi, > >We are a genomic research center in M=1B$B9^=1B(Bico and we will be in= itiating the >shotgun sequence of a large genome. Currently we do our bioinformatics= >in a 32 processor cluster, but we assemble (for example ESTs sequences= ) >using Phrap and/or CAP3. >The question is, >Can I parallelize the assembling of a shotgun sequencing project to us= e >the power of a cluster? > >Thanks in advance, > >Octavio. > >------------------------------------ >Octavio Mart=1B$B4Z=1B(Bez de la Vega >Profesor Titular >omartine@ira.cinvestav.mx > > -- Joseph Landman, Ph.D Scalable Informatics LLC, email: landman@scalableinformatics.com web : http://www.scalableinformatics.com phone: +1 734 612 4615 --__--__-- Message: 3 From: Charles Wust <charles@lisha.ufsc.br> Date: Tue, 31 Aug 2004 18:16:43 -0300 (BRT) Subject: Re: [Bioclusters] Parallel assembling of a shotgun genome To: bioclusters@bioinformatics.org Cc: bioclusters@bioinformatics.org, omartine@ira.cinvestav.mx Reply-To: bioclusters@bioinformatics.org Hello, I am presently doing research in this field, but I do not have any work= ing version of a parallel fragment assembler yet. But look for PCAP. It is made by the same author of CAP3, it should wor= k well on your cluster. Charles > Hi Octavio: > > Have a look at SPS Phrap Suite at > http://www.geospiza.com/tools/sps-phrap.htm . > > Joe > > Octavio Mart=1B$B4Z=1B(Bez de la Vega wrote: > >>Hi, >> >>We are a genomic research center in M=1B$B9^=1B(Bico and we will be i= nitiating the >>shotgun sequence of a large genome. Currently we do our bioinformatic= s >>in a 32 processor cluster, but we assemble (for example ESTs sequence= s) >>using Phrap and/or CAP3. >>The question is, >>Can I parallelize the assembling of a shotgun sequencing project to u= se >>the power of a cluster? >> >>Thanks in advance, >> >>Octavio. >> >>------------------------------------ >>Octavio Mart=1B$B4Z=1B(Bez de la Vega >>Profesor Titular >>omartine@ira.cinvestav.mx >> >> > > > -- > Joseph Landman, Ph.D > Scalable Informatics LLC, > email: landman@scalableinformatics.com > web : http://www.scalableinformatics.com > phone: +1 734 612 4615 > > _______________________________________________ > Bioclusters maillist - Bioclusters@bioinformatics.org > https://bioinformatics.org/mailman/listinfo/bioclusters > --__--__-- _______________________________________________ Bioclusters maillist - Bioclusters@bioinformatics.org https://bioinformatics.org/mailman/listinfo/bioclusters End of Bioclusters Digest = --1__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B Content-type: text/html; charset=ISO-2022-JP Content-Disposition: inline Content-transfer-encoding: quoted-printable <html><body> <p>How much is your large genome size(MB/GB)? The assembling method is = also decided by your genome size/repeats complexity. Using parallel phr= ap just can solve simple and small genome assembling.<br> <br> Email: zengjinz@cn.ibm.com <br> Notes: Zeng Jin Zhang/China/Contr/IBM@IBMCN<br> <img src=3D"cid:10__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com" width=3D= "16" height=3D"16" alt=3D"Inactive hide details for bioclusters-request= @bioinformatics.org">bioclusters-request@bioinformatics.org<br> <br> <br> <table width=3D"100%" border=3D"0" cellspacing=3D"0" cellpadding=3D"0">= <tr valign=3D"top"><td style=3D"background-image:url(cid:20__=3DC7BBE59= 0DF9A7F3B8f9e8a93df938@cn.ibm.com); background-repeat: no-repeat; " wid= th=3D"40%"> <ul> <ul> <ul> <ul><b><font size=3D"2">bioclusters-request@bioinformatics.org</font></= b><font size=3D"2"> </font><br> <font size=3D"2">Sent by: bioclusters-admin@bioinformatics.org</font> <p><font size=3D"2">2004-09-02 00:01</font> <table border=3D"1"> <tr valign=3D"top"><td width=3D"168" bgcolor=3D"#FFFFFF"><div align=3D"= center"><font size=3D"2">Please respond to<br> bioclusters</font></div></td></tr> </table> </ul> </ul> </ul> </ul> </td><td width=3D"60%"> <table width=3D"100%" border=3D"0" cellspacing=3D"0" cellpadding=3D"0">= <tr valign=3D"top"><td width=3D"1%" valign=3D"middle"><img src=3D"cid:3= 0__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com" border=3D"0" height=3D"= 1" width=3D"58" alt=3D""><br> <div align=3D"right"><font size=3D"2">To</font></div></td><td width=3D"= 100%"><img src=3D"cid:30__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com" = border=3D"0" height=3D"1" width=3D"1" alt=3D""><br> <font size=3D"2">bioclusters@bioinformatics.org</font></td></tr> <tr valign=3D"top"><td width=3D"1%" valign=3D"middle"><img src=3D"cid:3= 0__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com" border=3D"0" height=3D"= 1" width=3D"58" alt=3D""><br> <div align=3D"right"><font size=3D"2">cc</font></div></td><td width=3D"= 100%"><img src=3D"cid:30__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com" = border=3D"0" height=3D"1" width=3D"1" alt=3D""><br> </td></tr> <tr valign=3D"top"><td width=3D"1%" valign=3D"middle"><img src=3D"cid:3= 0__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com" border=3D"0" height=3D"= 1" width=3D"58" alt=3D""><br> <div align=3D"right"><font size=3D"2">Subject</font></div></td><td widt= h=3D"100%"><img src=3D"cid:30__=3DC7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.= com" border=3D"0" height=3D"1" width=3D"1" alt=3D""><br> <font size=3D"2">Bioclusters digest, Vol 1 #552 - 3 msgs</font></td></t= r> </table> <table border=3D"0" cellspacing=3D"0" cellpadding=3D"0"> <tr valign=3D"top"><td width=3D"58"><img src=3D"cid:30__=3DC7BBE590DF9A= 7F3B8f9e8a93df938@cn.ibm.com" border=3D"0" height=3D"1" width=3D"1" alt= =3D""></td><td width=3D"336"><img src=3D"cid:30__=3DC7BBE590DF9A7F3B8f9= e8a93df938@cn.ibm.com" border=3D"0" height=3D"1" width=3D"1" alt=3D""><= /td></tr> </table> </td></tr> </table> <br> <tt>Digest etiquette: When replying, PLEASE edit your Subject line so i= t<br> is more specific than "Re: Bioclusters digest, Vol..." = And, PLEASE<br> quote only related text -- do not quote the entire digest. Thank = you.<br> <br> <br> Today's Topics:<br> <br> 1. Parallel assembling of a shotgun genome (=3D?iso-8859-= 1?Q?Octavio_Mart=3DEDnez_de_la_Vega?=3D)<br> 2. Re: Parallel assembling of a shotgun genome (Joe Landm= an)<br> 3. Re: Parallel assembling of a shotgun genome (Charles W= ust)<br> <br> --__--__--<br> <br> Message: 1<br> From: =3D?iso-8859-1?Q?Octavio_Mart=3DEDnez_de_la_Vega?=3D <omartine= @ira.cinvestav.mx><br> To: <bioclusters@bioinformatics.org><br> Date: Tue, 31 Aug 2004 12:05:33 -0500<br> Subject: [Bioclusters] Parallel assembling of a shotgun genome<br= > Reply-To: bioclusters@bioinformatics.org<br> <br> Hi,<br> <br> We are a genomic research center in M=3DE9xico and we will be initiatin= g =3D<br> the<br> shotgun sequence of a large genome. Currently we do our bioinformatics<= br> in a 32 processor cluster, but we assemble (for example ESTs sequences)= <br> using Phrap and/or CAP3.<br> The question is,<br> Can I parallelize the assembling of a shotgun sequencing project to use= <br> the power of a cluster?<br> <br> Thanks in advance,<br> <br> Octavio.<br> <br> ------------------------------------<br> Octavio Mart=3DEDnez de la Vega<br> Profesor Titular<br> omartine@ira.cinvestav.mx<br> Km. 9.6 Lib. Nte., Carretera Irapuato-Le=3DF3n, Apartado Postal 629, CP= <br> 36500 Irapuato, Gto. M=3DE9xico.<br> tel: (55) 462 6239649<br> fax: (55) 462 6239660<br> mobile: 044 462 4822309<br> ------------------------------------<br> <br> <br> <br> --__--__--<br> <br> Message: 2<br> Date: Tue, 31 Aug 2004 13:12:09 -0400<br> From: Joe Landman <landman@scalableinformatics.com><br> To: bioclusters@bioinformatics.org, omartine@ira.cinvestav.mx<br> Subject: Re: [Bioclusters] Parallel assembling of a shotgun genom= e<br> Reply-To: bioclusters@bioinformatics.org<br> <br> Hi Octavio:<br> <br> Have a look at SPS Phrap Suite at <br> </tt><tt><a href=3D"http://www.geospiza.com/tools/sps-phrap.htm">http:/= /www.geospiza.com/tools/sps-phrap.htm</a></tt><tt> .<br> <br> Joe<br> <br> Octavio Mart韓ez de la Vega wrote:<br> <br> >Hi,<br> ><br> >We are a genomic research center in M閤ico and we will be ini= tiating the<br> >shotgun sequence of a large genome. Currently we do our bioinformat= ics<br> >in a 32 processor cluster, but we assemble (for example ESTs sequen= ces)<br> >using Phrap and/or CAP3.<br> >The question is,<br> >Can I parallelize the assembling of a shotgun sequencing project to= use<br> >the power of a cluster?<br> ><br> >Thanks in advance,<br> ><br> >Octavio.<br> ><br> >------------------------------------<br> >Octavio Mart韓ez de la Vega<br> >Profesor Titular<br> >omartine@ira.cinvestav.mx<br> > <br> ><br> <br> <br> -- <br> Joseph Landman, Ph.D<br> Scalable Informatics LLC,<br> email: landman@scalableinformatics.com<br> web : </tt><tt><a href=3D"http://www.scalableinformatics.com">htt= p://www.scalableinformatics.com</a></tt><tt><br> phone: +1 734 612 4615<br> <br> <br> --__--__--<br> <br> Message: 3<br> From: Charles Wust <charles@lisha.ufsc.br><br> Date: Tue, 31 Aug 2004 18:16:43 -0300 (BRT)<br> Subject: Re: [Bioclusters] Parallel assembling of a shotgun genom= e<br> To: bioclusters@bioinformatics.org<br> Cc: bioclusters@bioinformatics.org, omartine@ira.cinvestav.mx<br> Reply-To: bioclusters@bioinformatics.org<br> <br> Hello,<br> <br> I am presently doing research in this field, but I do not have any work= ing<br> version of a parallel fragment assembler yet.<br> <br> But look for PCAP. It is made by the same author of CAP3, it should wor= k<br> well on your cluster.<br> <br> Charles<br> <br> > Hi Octavio:<br> ><br> > Have a look at SPS Phrap Suite at<br> > </tt><tt><a href=3D"http://www.geospiza.com/tools/sps-phrap.htm">h= ttp://www.geospiza.com/tools/sps-phrap.htm</a></tt><tt> .<br> ><br> > Joe<br> ><br> > Octavio Mart韓ez de la Vega wrote:<br> ><br> >>Hi,<br> >><br> >>We are a genomic research center in M閤ico and we will be= initiating the<br> >>shotgun sequence of a large genome. Currently we do our bioinfo= rmatics<br> >>in a 32 processor cluster, but we assemble (for example ESTs se= quences)<br> >>using Phrap and/or CAP3.<br> >>The question is,<br> >>Can I parallelize the assembling of a shotgun sequencing projec= t to use<br> >>the power of a cluster?<br> >><br> >>Thanks in advance,<br> >><br> >>Octavio.<br> >><br> >>------------------------------------<br> >>Octavio Mart韓ez de la Vega<br> >>Profesor Titular<br> >>omartine@ira.cinvestav.mx<br> >><br> >><br> ><br> ><br> > --<br> > Joseph Landman, Ph.D<br> > Scalable Informatics LLC,<br> > email: landman@scalableinformatics.com<br> > web : </tt><tt><a href=3D"http://www.scalableinformatics.com= ">http://www.scalableinformatics.com</a></tt><tt><br> > phone: +1 734 612 4615<br> ><br> > _______________________________________________<br> > Bioclusters maillist - Bioclusters@bioinformatics.org<= br> > </tt><tt><a href=3D"https://bioinformatics.org/mailman/listinfo/bi= oclusters">https://bioinformatics.org/mailman/listinfo/bioclusters</a><= /tt><tt><br> ><br> <br> <br> <br> --__--__--<br> <br> _______________________________________________<br> Bioclusters maillist - Bioclusters@bioinformatics.org<br> </tt><tt><a href=3D"https://bioinformatics.org/mailman/listinfo/bioclus= ters">https://bioinformatics.org/mailman/listinfo/bioclusters</a></tt><= tt><br> <br> <br> End of Bioclusters Digest<br> </tt><br> </body></html>= --1__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B-- --0__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B Content-type: image/gif; name="graycol.gif" Content-Disposition: inline; filename="graycol.gif" Content-ID: <10__=C7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com> Content-transfer-encoding: base64 R0lGODlhEAAQAKECAMzMzAAAAP///wAAACH5BAEAAAIALAAAAAAQABAAAAIXlI+py+0PopwxUbpu ZRfKZ2zgSJbmSRYAIf4fT3B0aW1pemVkIGJ5IFVsZWFkIFNtYXJ0U2F2ZXIhAAA7 --0__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B Content-type: image/gif; name="pic31478.gif" Content-Disposition: inline; filename="pic31478.gif" Content-ID: <20__=C7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com> Content-transfer-encoding: base64 R0lGODlhWABDALP/AAAAAK04Qf79/o+Gm7WuwlNObwoJFCsoSMDAwGFsmIuezf///wAAAAAAAAAA AAAAACH5BAEAAAgALAAAAABYAEMAQAT/EMlJq704682770RiFMRinqggEUNSHIchG0BCfHhOjAuh EDeUqTASLCbBhQrhG7xis2j0lssNDopE4jfIJhDaggI8YB1sZeZgLVA9YVCpnGagVjV171aRVrYR RghXcAGFhoUETwYxcXNyADJ3GlcSKGAwLwllVC1vjIUHBWsFilKQdI8GA5IcpApeJQt8L09lmgkH LZikoU5wjqcyAMMFrJIDPAKvCFletKSev1HBw8KrxtjZ2tvc3d5VyKtCKW3jfz4uMKmq3xu4N0nK BVoJQmx2LGVOmrqNjjJf2hHAQo/eDwJGTKhQMcgQEEAnEjFS98+RnW3smGkZU6ncCWav/4wYOnAI TihRL/4FEwbp28BXMMcoscQCVxlepL4IGDSCyJyVQOu0o7CjmLN50OZlqWmyFy5/6yBBuji0AxFR M00oQAqNIstqI6qKHUsWRAEAvagsmfUEAImyxgbmUpJk3IklNUtJOUAVLoUr1+wqDGTE4zk+T6FG uQb3SizBCwatiiUgCBN8vrz+zFjVyQ8FWkOlg4NQiZMB5QS8QO3mpOaKnL0Z2EKvNMSILEThKhCg zMKPVxYJh23qm9KNW7pArPynMqZDiErsTMqI+LRi3QAgkFUbXpuFKhSYZALd0O5RKa2z9EYKBbpb qxIKsjUPRgD7I2XYV6wyrOw92ykExP8NW4URhknC5dKGE4v4NENQj2jXjmfNgOZDaXb5glRmXQ33 YEWQYNcZFnrYcIQLNzyTFDQNkXIff0ExVlY4srziQk43inZgL4rwxxINMvpFFAz1KOODHiu+4aEw NEjFl5B3JIKWKF3k6I9bfUGp5ZZcdunll5IA4cuHvQQJ5gcsoCWOOUwgltIwAKRxJgbIkJAQZEq0 2YliZnpZZ4BH3CnYOXldOUOfQoYDqF1LFHbXCrO8xmRsfoXDXJ6ChjCAH3QlhJcT6VWE6FCkfCco CgrMFsROrIEX3o2whVjWDjoJccN3LdggSGXLCdLEgHr1lyU3O3QxhgohNKXJCWv8JQr/PDdaqd6w 2rj1inLiGeiCJoDspAoQlYE6QWLSECehcWIYxIQES6zhbn1iImTHEQyqJ4eIxJJoUBc+3CbBuwZE V5cJPPkIjFDdeEabQbd6WgICTxiiz0f5dBKquXF6k4senwEhYGnKEFJeGrxUZy8dB8gmAXI/sPvH ESfCwVt5hTgYiqQqtdRNHQIU1PJ33ZqmzgE90OwLaoJcnMop1WiMmgkPHQRIrwgFuNV90A3doNKT mrKIN07AnGcI9BQjhCBN4RfA1qIZnMqorJCogKfGQnxSCDilTVIA0yl5ciTovgLuBDKFUDE9aQcw 9SA+rjSNf9/M1gxrj6VwDTS0IUSElMzBfsj0NFXR2kwsV1A5IF1grLgLL/r1R40BZEnuBWgmQEyb jqRwSAt6bqMCOFkvKFN2GPPkUzIm/SCF8z8pVzpbjVnMsy0vOr1hw3SaSRUhpY09v0z0J1FnwzPl fmh+xl4WtR0zGu24I4KbMQm3lnVu2oNWxI9W/lcyzA+mCKF4DBikxb/+UWtOGRiFP8qEwAayIgIA Ow== --0__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B Content-type: image/gif; name="ecblank.gif" Content-Disposition: inline; filename="ecblank.gif" Content-ID: <30__=C7BBE590DF9A7F3B8f9e8a93df938@cn.ibm.com> Content-transfer-encoding: base64 R0lGODlhEAABAIAAAAAAAP///yH5BAEAAAEALAAAAAAQAAEAAAIEjI8ZBQA7 --0__=C7BBE590DF9A7F3B8f9e8a93df938690918cC7BBE590DF9A7F3B--