[Bioclusters] sensitivity & blast
Pamela Culpepper
pculpep at hotmail.com
Wed Apr 6 16:39:12 EDT 2005
Lik Mui,
More testing reveals that the -Y option works as follows.
In the absense of -Y, the "effective search space" is the product of the
query sequence length
and the total database length. It affects the calculation of the expection
value but not the score.
It will thus vary with the query sequence length.
Using "-Y 12345" sets the above "effective search space" to 12345, constant
for each query
sequence. To make the expectation roughly reflect match length, one could
use a -Y value
that is the product of the database size and the longest query sequence
size.
Pam and Bill
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