Greetings This question appears to have come up before on these lists, but I don't see an answer. I recall we've posted about this problem before but I don't think we ever got the problem resovled. I created two databases using the commands below from the Ensembl human and mouse databases. I tried to run a query with: setenv data_root /scratch/uahmam setenv blast_root /opt/asn/apps/mpiBlast_1.3.0/latest/bin setenv MPI_REQUEST_MAX 128000 setenv MPI_TYPE_MAX 128000 echo "blast_root is "$blast_root echo "data_root is "$data_root cd /scratch/uahmam time /usr/bin/mpirun -np 8 $blast_root/mpiblast --remove -p blastn -d Homo_sapiens.NCBI35.nov.cdna.fa -i novel_v11.fa -W 9 -r 1 -q -1 -G 1 -E 3 -o novel_v_Hsen.blast where my .ncbirc file is as below: [mpiBLAST] Shared=/scratch/uahmam Local=/scratch/uahmam [NCBI] Data= /opt/asn/apps/ncbi_6.1/data [BLAST] BLASTDB=/scratch/uahmam BLATMAT=/opt/asn/apps/ncbi_6.1/data mpiformatdb --nfrags=6 -t enHs -i Homo_sapiens.NCBI35.nov.cdna.fa -p F -n enHs mpiformatdb --nfrags=6 -t enMm -i Mus_musculus.NCBIM34.nov.cdna.fa -p F -n enMm I get nothing but a million+ lines of: ObjMgrNextAvailEntityID failed with idx 2048 It fills up my disk quota and crashes so I get nothing else. I have found this line in the source code, but I can't figure out what's going on from that. mpiblast seems to work OK for other databases/queries. Can anybody offer any suggestions? Thanks Mike