Joe Landman wrote: > Daniel.G.Roberts at aventis.com wrote: > >> Hello All >> Can anyone point me to example/FAQ resources on BLAST implemented on a >> Linux Cluster? >> > It depends on your blast job. There are two ways to accelerate: If you have thousand of sequences then the best way is to have a dedicated NFS server running of gigabit LAN and each node with ample amount of RAM. Use NCBI blast through a good job scheduler like SGE and throw each sequences as a separte blast job with BLAST database shared over Gigabit LAN. From day to day experience I can say a routine NFS mount can use around ~250 sequences at a time. If you have very few sequences and want to just run a single job as fast as possible, the best way is to split the database. mpiBLAST is best known for that or you can even use your own custom script. Remember that it will screw up the BLAST statistics. -Malay mbasu(at)ncbi.nlm.nih.gov