Dear Sir, I am biological science. I am interested in mpiBLAST. I try to install by following the README file of mpiblast v1.3.0 (window version). I think the installation should be work but when I ran the command line > mpiformatdb -N 25 -i nt at the shared directory the Fatal Error occurred: Unable to read mpiBlast shared storage path from either .ncbirc or the MPIBLAST_SHARED environment variable. The mpiblast configuration in .ncbirce should look like [mpiBLAST] Shared=/path/to/shared/storage Loca=/path/to/local/storage I don't know how to solve this problem. I tried in three days ago. I think I install mpiblast in window so it should not relate to .ncbirc (config file in unix). How ever I don't know about the MPIBLAST_SHARED environment variable because the installation does not suggest me about this. - Is my installation correct? 1. install mpich-nt. 2. Copy mpiblast.exe and mpiformatdb.exe to a shared storage directory, C:\Documents and Settings\All Users\shereForMPIblast 3. Create a ncbi.ini configuration file created in the Windows directory,C:\Windows: [NCBI] Data=C:\Documents and Settings\All Users\shereForMPIblast [BLAST] BLASTDB=C:\Documents and Settings\All Users\shereForMPIblast BLASTMAT=C:\Documents and Settings\All Users\shereForMPIblast [mpiBLAST] Shared=C:\Documents and Settings\All Users\shereForMPIblast Local=C:\Program Files\mpiBLAST ** for the Local path, it is the path that mpiblast v1.3 is installed. 4. Get the NCBI data distribution (ncbi.tar) and copy its contents to C:\Documents and Settings\All Users\shereForMPIblast 5. Edit the file mpiblast CONF file (after the eorror occured) at C:\Program Files\mpiBLAST like [mpiBLAST] Shared=C:\Documents and Settings\All Users\shereForMPIblast Local=C:\Program Files\mpiBLAST *** In this work I installed mpiBlast program in a single computer (just my own computer only) no linking to other computer. Can the problem occur fromthis case? Please help me. Thank you very much. Best regards, Amornpan. --------------------------------- Do you Yahoo!? Read only the mail you want - Yahoo! Mail SpamGuard. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://bioinformatics.org/pipermail/bioclusters/attachments/20050609/a2426a9a/attachment.htm