Dinanath Sulakhe wrote: > At 05:06 PM 3/9/2005, Lucas Carey wrote: > >> Hi Dina, >> I don't know how many of the results you actually need. You may free >> up some memory by limiting e-value, returned results, and aligned >> results. >> blastall -e 0.0001 -b 25 -v 25 > > > would limiting the e-value and other parameters reduce the RAM usage?? probably. blastall processes one query at a time so its memory requirements are determined by the largest result size of any one query in your data set. The b/v parameters mentioned by Lucas limit the number of results reported for each query, which ought to limit the memory consumption of blastall.