Dinanath Sulakhe wrote: > Now the Admin of the Site is asking me if its possible to reduce the > amount of memory these blast jobs use? He says these jobs are requesting > about 600-700MB of RAM, and he is asking me to reduce it to atmost 500MB. > > Is it possible to reduce the amount of RAM it is requesting by tweaking > any of the parameters in blast?? Yes. Run formatdb similar to this formatdb -i $db -o T -p T -v 200 where $db is the name of your db as in your blast line below. This will segment your database down quite a bit. Note: there are limits you would have to worry about if $db is huge (over 10 GB or so). > > My blast options are : > > blastall -i $input -o $output -d $db -p blastp -m 8 -F F > > Please let me know, > Thank you, > Dina > > _______________________________________________ > Bioclusters maillist - Bioclusters at bioinformatics.org > https://bioinformatics.org/mailman/listinfo/bioclusters -- Joseph Landman, Ph.D Founder and CEO Scalable Informatics LLC, email: landman at scalableinformatics.com web : http://www.scalableinformatics.com phone: +1 734 786 8423 fax : +1 734 786 8452 cell : +1 734 612 4615