Dear Sir, Thanks for your kind assitance. I have initiated the mpiblast successfully yesterday. While running I have faced some error message. It is as follows: 1. Process: ------------ NCBI: /apps/Software/NCBI-2.2.10-5.x86_64.rpm mpiblast: /apps/Software/mpiblast-1.3.0-2LAM.x86_64.rpm .ncbirc: [NCBI] Data=/apps/Databases/ncbi/data ( In this directory all the BLOSUM and other ncbi data is there) [BLAST] BLASTDB=/apps/Databases/blastdb ( Its aimed to database created by mpiformatdb at /apps/Databases/blastdb) BLASTMAT=/apps/Databases/ncbi/data ( It also to ncbidata). [mpiBLAST] Shared=/apps/Databases/blastdb ( full permission) Local=/tmp/mpiblast(full permission) ----------------------------------------------------- in /home/sravan: mpiformatdb -N 250 -i /apps/Databases/silkbase/scaffolds(368MB) -p F : has created a dataabase in /apps/Database/blastdb Then it has given: it has created a database with scaffolds(368MB) - 250 fragments each with 2MB. Script: /usr/local/lam/bin/mpirun -np 3 /usr/local/bin/mpiblast -p blastn -i <input> -d <db> -o mpiblast.out Error Message: ------------- The sequence file has 1000 sequences of different sizes and as whole has 490kb. When I started the script , it ran properly upto 90 or 100 se quences. Then also it continued. But during the process it has giving the message serially: [Blastall] ERROR: ncbiapi [000.000] ObjMgrNextAvailEntityID failed with idx 2048. Kindly assist me in resolving this problem. Thank you very much. Regards. Sravan. ============================================= P.Sravana Kumar. Laboratory of Molecular Genetics. Center for DNA Fingerprinting and Diagnostics. ECIL road, Nacharam. Hyderabad 500 076. Ph: 91-40-27151344-1102. Ph: 91-40-27151344-1209. e-mail: sravan at cdfd.org.in,sravan_mar at yahoo.com ==============================================