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<font face="Arial, Helvetica" size=4><b>Gene Quantification
Newsletter January 2003<br>
<br>
</b></font><font face="Arial, Helvetica">Happy New Year 2003<br>
to all Newsletter Readers and Gene Quantification Visitors<br>
<br>
<br>
</font><font face="Arial, Helvetica" size=4><b>NEW features:<br>
<br>
</font><font face="Arial, Helvetica">RNA and Gene Expression
- NEW PUBLICATIONS <br>
<br>
</b>Tissue-specific expression pattern of bovine prion gene (PrP):
Quantification using real-time RT-PCR<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/absolute.html#prp" eudora="autourl">http://www.wzw.tum.de/gene-quantification/absolute.html#</a><a href="http://www.wzw.tum.de/gene-quantification/absolute.html#prp" eudora="autourl">prp<br>
<br>
</a></u></font><font face="Arial, Helvetica">Detection and quantification
of mRNA-expression of alpha- and beta-adrenergic <br>
receptor subtypes in the mammary gland of dairy cows.<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg" eudora="autourl">http://www.wzw.tum.de/gene-quantification/endo.html#</a><a href="http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg" eudora="autourl">adrenerg<br>
<br>
</a></u></font><font face="Arial, Helvetica">Gene Quantification in
Endocrinology - NEW PUBLICATIONS <br>
Gene Expression of Immunologically Important Factors in Blood Cells, Milk
Cells and Mammary Tissue of Cows.<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/immuno.html#papers" eudora="autourl">http://www.wzw.tum.de/gene-quantification/immuno.html#papers<br>
<br>
</a></u></font><font face="Arial, Helvetica">Insulin-like growth factor
and insulin receptors in intestinal mucosa of neonatal calves.<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/endo.html#igfs" eudora="autourl">http://www.wzw.tum.de/gene-quantification/endo.html#</a><a href="http://www.wzw.tum.de/gene-quantification/endo.html#igfs" eudora="autourl">igfs<br>
<br>
<br>
</a></u></font><font face="Arial, Helvetica"><b>Determination of
real-time PCR efficiency<br>
(also in competitive RT-PCR)<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/efficiency.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/efficiency.</a><a href="http://www.wzw.tum.de/gene-quantification/efficiency.html" eudora="autourl">html<br>
<br>
</a></u></font><font face="Arial, Helvetica">NEW EQUATIONS &
ALGORITHM by Tichopad et al. 2002<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2" eudora="autourl">http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2<br>
<br>
<br>
</a></u></font><font face="Arial, Helvetica"><b>NEW MOVIES<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/movie.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/movie.</a><a href="http://www.wzw.tum.de/gene-quantification/movie.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">e.g. Molecular
Biology, PCR, RPA, Electrophoresis, Northern- & Southern-Blot<br>
<br>
<br>
<b>NEW PUBLICATIONS about kinetic PCR<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/real-time.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/real-time.</a><a href="http://www.wzw.tum.de/gene-quantification/real-time.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">and<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/literature.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/literature.</a><a href="http://www.wzw.tum.de/gene-quantification/literature.html" eudora="autourl">html<br>
<br>
<br>
</a></u></font><font face="Arial, Helvetica"><b>Poster boards (a little
bit slow !!!):<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/poster-a.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/poster-a.</a><a href="http://www.wzw.tum.de/gene-quantification/poster-a.html" eudora="autourl">html<br>
</a></u></font><font face="Arial, Helvetica">methodological poster
board A: Array verification <br>
physiological poster board B: IGFs - Early
Response Genes <br>
physiological poster board C: Steroid
receptors <br>
physiological poster board D: Zinc
metabolism <br>
physiological poster board E: Immunological
relevant factors<br>
<br>
<b>New links & databases:<br>
</b></font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/link.html" eudora="autourl">http://www.wzw.tum.de/gene-quantification/link.</a><a href="http://www.wzw.tum.de/gene-quantification/link.html" eudora="autourl">html<br>
</a><a href="http://www.realtimeprimerdatabase.ht.st/" eudora="autourl">http://www.realtimeprimerdatabase.ht.</a><a href="http://www.realtimeprimerdatabase.ht.st/" eudora="autourl">st<br>
</a><a href="http://www.realtimeprimers.org/" eudora="autourl">http://www.realtimeprimers.org/<br>
</a><a href="http://www.pcrlinks.com/" eudora="autourl">http://www.pcrlinks.</a><a href="http://www.pcrlinks.com/" eudora="autourl">com<br>
</a><a href="http://www.pcrlinks.com/variants/real-time_pcr.htm" eudora="autourl">http://www.pcrlinks.com/variants/real-time_pcr.</a><a href="http://www.pcrlinks.com/variants/real-time_pcr.htm" eudora="autourl">htm<br>
</a><a href="http://www.cyclertest.com/" eudora="autourl">http://www.cyclertest.com/<br>
<br>
</a></u></font><font face="Arial, Helvetica">The COMPLETE Alkami Quick
GuideTM for PCR is a (158 pages)<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf" eudora="autourl">http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.</a><a href="http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf" eudora="autourl">pdf<br>
<br>
</a></u></font><font face="Arial, Helvetica">EUROGENTEC PCR manual
(48 pages)<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/eurogentec-qq-booklet.pdf" eudora="autourl">http://www.wzw.tum.de/gene-quantification/eurogentec-qq-booklet.</a><a href="http://www.wzw.tum.de/gene-quantification/eurogentec-qq-booklet.pdf" eudora="autourl">pdf<br>
<br>
</a></u></font><font face="Arial, Helvetica">LightCycler University
(LCU)<br>
</font><font face="Arial, Helvetica" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/real-time.html#lcu" eudora="autourl">http://www.wzw.tum.de/gene-quantification/real-time.html#</a><a href="http://www.wzw.tum.de/gene-quantification/real-time.html#lcu" eudora="autourl">lcu<br>
<br>
</a></u></font><font face="Arial, Helvetica"><b>Please send the
NEWSLETTER and the home page links to further scientists and friends who
are interested in real-time PCR, new quantification strategies, relative
or absolute gene quantification using kinetic PCR.<br>
<br>
</b>best regards<br>
<br>
Michael Pfaffl<br>
<br>
#############################################<br>
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********************************************************************<br>
<font size=5><b>Gene Quantification @ wzw.tum.de<br>
<br>
</b></font><font color="#0000FF">e-mail:<x-tab> </x-tab>gene.quantification@wzw.tum.de
<br>
homepage:<x-tab> </x-tab><a href="http://www.wzw.tum.de/gene-quantification/index.shtml" eudora="autourl">http://www.wzw.tum.de/gene-quantification/index.shtml</a><br>
homepage:<x-tab> </x-tab><a href="http://www.wzw.tum.de/gene-quantification/physiology.shtml" eudora="autourl">http://www.wzw.tum.de/gene-quantification/physiology.shtml</a><br>
<br>
</font><u>responsible Senior Scientist:<br>
</u>Dr. Michael W. Pfaffl<br>
Institute of Physiology<br>
Life Science Center Weihenstephan,<br>
Technical University of Munich<br>
Weihenstephaner Berg 3<br>
D-85350 Freising-Weihenstephan<br>
GERMANY<br>
<br>
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