PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
Home | Help | Analyzed genomes
 
A) Input parameters
Genome2201.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_006511 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1SPA0008SPA0034Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA00082240.019720molybdopterin biosynthesis Mog protein
SPA0009117-2.349965hypothetical protein
SPA0010216-3.334979hypothetical protein
SPA0011016-3.845696hypothetical protein
SPA0012017-4.810924DnaK protein (heat shock protein 70)
SPA0013-217-3.439914DnaJ protein
SPA0014-119-4.580766regulatory protein
SPA0015-217-2.900491hypothetical protein
SPA0016-116-2.240189hypothetical protein
SPA0017016-1.227942hypothetical protein
SPA0019014-0.694872hydroxymethyltransferase
SPA0020115-1.865167hypothetical protein
SPA0021215-0.932423fimbrial subunit
SPA0022117-0.634060fimbrial chaperone
SPA0023118-0.976765fimbrial usher
SPA0024125-4.026011fimbrial subunit
SPA0025-131-7.108225fimbrial subunit
SPA0027032-8.613609fimbrial chaperone
SPA0028030-9.579482hypothetical protein
SPA0029125-10.400724hypothetical protein
SPA0030123-10.175672hypothetical protein
SPA0031119-8.598422LysR family transcriptional regulator
SPA0032116-6.791080transcriptional regulator
SPA0033214-5.416093sulfatase
SPA0034-111-3.6826035'-nucleotidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0012SHAPEPROTEIN1413e-39 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 141 bits (357), Expect = 3e-39
Identities = 84/388 (21%), Positives = 152/388 (39%), Gaps = 86/388 (22%)

Query: 5 IGIDLGTTNSCVAIMDGTQARVLENAEGDRTTPSIIAYTQDGET------LVGQPAKRQA 58
+ IDLGT N+ + + Q VL PS++A QD VG AK+
Sbjct: 13 LSIDLGTANTLIYVKG--QGIVLNE-------PSVVAIRQDRAGSPKSVAAVGHDAKQML 63

Query: 59 VTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPYKIIGADNGDAWLDVKGQKMAPPQISAE 118
P N + AI+ +K +A ++ +
Sbjct: 64 GRTPGN-IAAIR---------------------------------PMKDGVIADFFVTEK 89

Query: 119 VLKK-MKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAAL 177
+L+ +K+ + P ++ VP +R+A +++ + AG +I EP AAA+
Sbjct: 90 MLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAI 149

Query: 178 AYGL--DKEVGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDTR 235
GL + G+ V D+GGGT ++++I ++ V + +GG+ FD
Sbjct: 150 GAGLPVSEATGS---MVVDIGGGTTEVAVISLNGV---------VYSSSVRIGGDRFDEA 197

Query: 236 LINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA----QQTDVNLPYITADAT 291
+INY+ + G + AE+ K E+ SA + ++ +
Sbjct: 198 IINYVRRNYGSLIG-------------EATAERIKHEIGSAYPGDEVREIEVRGRNLAEG 244

Query: 292 GPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQD-AGLSVSDIND--VILVGGQTRMPM 348
P+ + + LE+L E + + + VAL+ SDI++ ++L GG +
Sbjct: 245 VPRGFTLN-SNEILEALQEP-LTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRN 302

Query: 349 VQKKVAEFFGKEPRKDVNPDEAVAIGAA 376
+ + + E G +P VA G
Sbjct: 303 LDRLLMEETGIPVVVAEDPLTCVARGGG 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0023PF005778320.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 832 bits (2150), Expect = 0.0
Identities = 439/873 (50%), Positives = 583/873 (66%), Gaps = 27/873 (3%)

Query: 14 VAKPVLTPLALAIALAPA------PGWAENYFNPAFLSDDPSAVADLSTFSR-NAQAAGM 66
+ K L + + +A A AE YFNP FL+DDP AVADLS F G
Sbjct: 18 IRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGT 77

Query: 67 YRVDVYLNNTFLATRDIAFQAVKTTGKSAPTDDSGLRACLTPEMLKNMGVNTGAFPLLAK 126
YRVD+YLNN ++ATRD+ F + G+ CLT L +MG+NT + +
Sbjct: 78 YRVDIYLNNGYMATRDVTFNTGD--------SEQGIVPCLTRAQLASMGLNTASVSGMNL 129

Query: 127 AAAGSCPDLASAIPAARTRFDFAQQRLDISIPQAAMVASARGYIPPQYWDEGINALLFNY 186
A +C L S I A + D QQRL+++IPQA M ARGYIPP+ WD GINA L NY
Sbjct: 130 LADDACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNY 189

Query: 187 TFTGANSQDRSPGGSAENSYFLGLNSGLNLGAWRLRDYSTWNANSGDQNSDS--DWQHIS 244
F+G + Q+R G S + +L L SGLN+GAWRLRD +TW+ NS D +S S WQHI+
Sbjct: 190 NFSGNSVQNRIGGNS--HYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN 247

Query: 245 THLERDVVFLQGELTAGDSYTPSALFDSLPFRGLQLASDDNMLPDSMKGFAPTIHGIARS 304
T LERD++ L+ LT GD YT +FD + FRG QLASDDNMLPDS +GFAP IHGIAR
Sbjct: 248 TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARG 307

Query: 305 NAQVTIRQNGYIINQRYVPPGAFTINDLYPTAASGDLTVEVKESDGSINRYNVPYSAVPI 364
AQVTI+QNGY I VPPG FTIND+Y SGDL V +KE+DGS + VPYS+VP+
Sbjct: 308 TAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPL 367

Query: 365 LQREGRLKYAATVAEYRSDSSQKEKVKFSQATLIWGLPHGFTLYGGTQLSSHYHALAIGS 424
LQREG +Y+ T EYRS ++Q+EK +F Q+TL+ GLP G+T+YGGTQL+ Y A G
Sbjct: 368 LQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGI 427

Query: 425 GANLGDWGAVSLDVTQATSTLADNNTYQGQSLRFLYAKSLAQSGTNLQLMGYRYSTSGFY 484
G N+G GA+S+D+TQA STL D++ + GQS+RFLY KSL +SGTN+QL+GYRYSTSG++
Sbjct: 428 GKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYF 487

Query: 485 TLDDTTWKRMSGYDDDNRTDSDKSRPEWADYYNLYYTRRGKVQLDINQQLGGLGSLFITG 544
DTT+ RM+GY+ + + + +P++ DYYNL Y +RGK+QL + QQLG +L+++G
Sbjct: 488 NFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSG 547

Query: 545 SQQSYWHTDEKDSLLQVGYSDTLAGIAWSVSYNNNKSAGDAERDQIFALNISVPLSQWLQ 604
S Q+YW T D Q G + I W++SY+ K+A RDQ+ ALN+++P S WL+
Sbjct: 548 SHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLR 607

Query: 605 HDDEVTHHHNVYATFSTSTDKQHNVTQNAGLSGTLLDENNLSYNIQQGYQNHGIGESGA- 663
D + + A++S S D +T AG+ GTLL++NNLSY++Q GY G G SG+
Sbjct: 608 SDS-KSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGST 666

Query: 664 --ASLEYDGAKGNANIGYNVSDNGDYQQVNYGLSGGLVAHAHGVTLSQPLGNTNILIAAP 721
A+L Y G GNANIGY S + D +Q+ YG+SGG++AHA+GVTL QPL +T +L+ AP
Sbjct: 667 GYATLNYRGGYGNANIGY--SHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAP 724

Query: 722 GAANVGVVDQPGIHTDARGYAVVPYATTYRQNRMALDVNAMADDVDIDDAVTRVVPTEGA 781
GA + V +Q G+ TD RGYAV+PYAT YR+NR+ALD N +AD+VD+D+AV VVPT GA
Sbjct: 725 GAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGA 784

Query: 782 LVLARFKARVGVRALVTLNHNGKPVPFGATVTVNDRHAEAIVDEAGEVYLSGLSAQGVLH 841
+V A FKARVG++ L+TL HN KP+PFGA VT + IV + G+VYLSG+ G +
Sbjct: 785 IVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQ 844

Query: 842 VRWGNLPDQQCVASYHL--SSSRQILSRQHAEC 872
V+WG + CVA+Y L S +Q+L++ AEC
Sbjct: 845 VKWGEEENAHCVANYQLPPESQQQLLTQLSAEC 877


2SPA0052SPA0065Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA00520253.738715nucleoside hydrolase
SPA0053-1242.495726transcriptional regulatory protein citb
SPA0054-1232.928651transcriptional regulator
SPA00550275.129995oxaloacetate decarboxylase subunit beta
SPA0056-2193.243005oxaloacetate decarboxylase subunit alpha
SPA0057-1130.253352oxaloacetate decarboxylase subunit gamma
SPA0058-1110.408433citrate-sodium symporter
SPA00590101.704970[citrate (pro-3S)-lyase] ligase
SPA0060-1102.132702citrate lyase acyl carrier protein
SPA0061-1112.055702citrate lyase subunit beta
SPA00620203.562114citrate lyase subunit alpha
SPA00630203.227669hypothetical protein
SPA00640203.164783CitG protein
SPA00650233.315664dihydrodipicolinate reductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0053HTHFIS697e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 69.1 bits (169), Expect = 7e-16
Identities = 29/141 (20%), Positives = 48/141 (34%), Gaps = 2/141 (1%)

Query: 1 MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLP 60
M T L+ +D+ + +L L V I + + I L++ D +P
Sbjct: 1 MTGATILVADDDAAIRTVLNQA--LSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 DGKGIDLIRHTISTNYTGRIIFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERF 120
D DL+ ++ ++A N T A G +DYL KP L + R
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 121 TRYRSSLRSSEQANQTHVDAL 141
S + + L
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0054CARBMTKINASE300.017 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 30.2 bits (68), Expect = 0.017
Identities = 19/81 (23%), Positives = 30/81 (37%), Gaps = 13/81 (16%)

Query: 91 DATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPI 150
+ + G EK Q L V +E+ KY E G + GS+G +
Sbjct: 238 NGAALYYGTEKEQWLREVKVEELRKYYEEG-------------HFKAGSMGPKVLAAIRF 284

Query: 151 QDSTGKVIGIVSVGYTLEQLE 171
+ G+ I + +E LE
Sbjct: 285 IEWGGERAIIAHLEKAVEALE 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0056RTXTOXIND320.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.7 bits (72), Expect = 0.007
Identities = 16/58 (27%), Positives = 28/58 (48%)

Query: 507 ASAPAAAAPAGAGTPVTAPLAGNIWKVIATEGQTVAEGDVLLILEAMKMETEIRAAQA 564
A+A +G + + ++I EG++V +GDVLL L A+ E + Q+
Sbjct: 84 ATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQS 141



Score = 29.8 bits (67), Expect = 0.034
Identities = 14/56 (25%), Positives = 21/56 (37%), Gaps = 10/56 (17%)

Query: 532 KVIATEGQTVAEGDVLLILEAMKMETEIRAAQAGTVRGIAVKSGDAVSVGAPLMTL 587
V G+ G EI+ + V+ I VK G++V G L+ L
Sbjct: 82 IVATANGKLTHSGRSK----------EIKPIENSIVKEIIVKEGESVRKGDVLLKL 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0059LPSBIOSNTHSS403e-06 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 40.2 bits (94), Expect = 3e-06
Identities = 21/102 (20%), Positives = 42/102 (41%), Gaps = 4/102 (3%)

Query: 158 NPFTLGHRYLVEQAAAACDWLHLFVLKEDAS--FFSYTDRWALIEQGIAGIDNVTLHPGS 215
+P T GH ++E+ D +++ VL+ FS +R I + IA + N +
Sbjct: 10 DPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLPNAQVDSFE 69

Query: 216 AYMISRATFPGYFLKEKGV--VDDCHCQIDLQLFREHLAPAL 255
++ A +G+ + D ++ + + LA L
Sbjct: 70 GLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDL 111


3SPA0103SPA0109Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0103-2173.867512L-ribulose-5-phosphate 4-epimerase
SPA01040174.638373L-arabinose isomerase
SPA01051164.638741L-ribulokinase
SPA01061173.673894arabinose operon regulatory protein
SPA01071163.672114DedA family integral membrane protein
SPA01080164.088007ABC transporter
SPA0109-1173.916445ABC transporter integral membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0109PF06580310.017 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.6 bits (69), Expect = 0.017
Identities = 16/79 (20%), Positives = 27/79 (34%), Gaps = 3/79 (3%)

Query: 4 RRQPLIPGWLIPGLCAAALMITVSLAAFLALWLNAPSGAWSTLWRDSYLWHVVRFSFWQA 63
R GWL + L + + +W A + W L +++
Sbjct: 60 RSFIKRQGWLKLNMGQIILRVLPACVVIGMVWFVANTSIWRLL---AFINTKPVAFTLPL 116

Query: 64 FLSAVLSVVPAVFLARALY 82
LS + +VV F+ LY
Sbjct: 117 ALSIIFNVVVVTFMWSLLY 135


4SPA0152SPA0162Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA01522261.025243glycosyl hydrolase
SPA01533321.058873symporter
SPA01544361.744676aromatic amino acid transport protein AroP
SPA01554361.860650pyruvate dehydrogenase complex repressor
SPA01562302.170060pyruvate dehydrogenase E1 component
SPA01572273.326244dihydrolipoamide acetyltransferase component
SPA01580223.349321dihydrolipoamide dehydrogenase
SPA0159-2154.091090secreted protein
SPA0160-2144.103358secreted protein
SPA0161-2133.868302hypothetical protein
SPA0162-1153.605396aconitate hydratase 2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0157RTXTOXIND357e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 35.2 bits (81), Expect = 7e-04
Identities = 40/281 (14%), Positives = 85/281 (30%), Gaps = 32/281 (11%)

Query: 26 DKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGALIMIFDSADGAADAAP 85
+ V +T G S E+ + +VKEI V G+ G +++ + AD
Sbjct: 81 EIVATANGKLTHSGR--SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 86 AKA--------EEKKEAAPAAAPAAAAAKDVHVPDIGSDEVEVTEVMVKVG------DTV 131
++ + + + + + + V EV+ T
Sbjct: 139 TQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTW 198

Query: 132 EAEQSLITVEGDKASMEVPAPFAGTVKEIKVNTGDKVSTGSLIMVFEVAGEAGAAAPAKA 191
+ ++ + DK E A + ++ +K + A +
Sbjct: 199 QNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHK-QAIAKHAVLEQ 257

Query: 192 EAAPAAAAPAAATGVKDVNVPDIGGDEV-------------EVTEVMVKVGDKVAA-EQS 237
E A + + E+ + + + D +
Sbjct: 258 ENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLE 317

Query: 238 LITVEGDKASMEVPAPFAGTVKEIKIST-GDKVKTGSLIMV 277
L E + + + AP + V+++K+ T G V T +MV
Sbjct: 318 LAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMV 358



Score = 29.8 bits (67), Expect = 0.040
Identities = 16/85 (18%), Positives = 32/85 (37%), Gaps = 4/85 (4%)

Query: 230 DKVAAEQSLITVEGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAP 289
+ VA +T G S E+ VKEI + G+ V+ G ++ ++ A
Sbjct: 81 EIVATANGKLTHSGR--SKEIKPIENSIVKEIIVKEGESVRKGDVL--LKLTALGAEADT 136

Query: 290 AKQEAAAPAPAAKAEKPAAPAAKAE 314
K +++ + + + E
Sbjct: 137 LKTQSSLLQARLEQTRYQILSRSIE 161


5SPA0177SPA0209Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0177118-3.788667PTS system transporter subunit IIA
SPA0178216-3.007214hypothetical protein
SPA0179320-3.427571fimbrial protein
SPA0180320-3.889893fimbrial protein
SPA0181220-3.572743fimbrial protein
SPA0182017-2.911548fimbrial protein
SPA0183017-1.549602outer membrane usher protein
SPA0184-217-0.556674fimbriae; chaparone
SPA0185-2191.568043major fimbrial subunit
SPA0186-1183.188113aspartate 1-decarboxylase
SPA0187-1193.159272pantoate:beta-alanine ligase
SPA0188-2162.4598113-methyl-2-oxobutanoate
SPA0189-1152.7649842-amino-4-hydroxy-6-
SPA01900113.802426poly(A) polymerase
SPA0191-2133.787922glutamyl-tRNA synthetase
SPA0192-2133.942554dosage-dependent dnaK suppressor protein
SPA0193-2144.342948sugar fermentation stimulation protein
SPA0194-1175.6823802'-5' RNA ligase
SPA0195-2175.115934ATP-dependent helicase HrpB
SPA0196-2174.364326penicillin-binding protein 1b; peptidoglycan
SPA01981174.656909ferrichrome transport ATP-binding protein FhuC
SPA01990153.807309ferrichrome-binding periplasmic protein
SPA0200-1142.091733ferrichrome transport protein FhuB precursor
SPA0201-1130.439094fimbrial subunit
SPA02030121.575805periplasmic fimbrial chaperone
SPA02041131.283329minor fimbrial subunit
SPA02061131.407851minor fimbrial subunit
SPA02070121.782947hypothetical protein
SPA02081153.106768glutamate-1-semialdehyde 2,1-aminomutase
SPA02092140.396242hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0183PF005777740.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 774 bits (2000), Expect = 0.0
Identities = 267/879 (30%), Positives = 449/879 (51%), Gaps = 34/879 (3%)

Query: 2 LFQRSLLCLTIG----AALPFSVSAANSAAEKTVVESDEAVEFNEQFLLNSS-ANIDISR 56
L+QR+ CL I A + A + A S + FN +FL + A D+SR
Sbjct: 8 LYQRNTQCLHIRKHRLAGFFVRLFVACAFAA-QAPLSSAELYFNPRFLADDPQAVADLSR 66

Query: 57 YAYGNPVLAGTYRVKVNLNNALKSTSEITFNEN-GTPRASACLTPLLLTQAGVDPAAMRD 115
+ G + GTYRV + LNN +T ++TFN CLT L G++ A++
Sbjct: 67 FENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSG 126

Query: 116 DVEVDDNTTCLDIKKYYPGATANYDSGKQAMDLNFPQIYILKRPAGYVDPSLWEDGVPAA 175
+ D+ C+ + ATA D G+Q ++L PQ ++ R GY+ P LW+ G+ A
Sbjct: 127 MNLLADDA-CVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAG 185

Query: 176 IVSYDMNAWHSEGN-GTTSDTAYVELRYGLNMGPWRLRSRGSLNWNKDTGS-----EYNN 229
+++Y+ + + G S AY+ L+ GLN+G WRLR + ++N S ++ +
Sbjct: 186 LLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQH 245

Query: 230 QDVYLQRDITALKAQMVIGDSYTRGDAFDSFSLSGIRMYNDDRMLPMGSSNYAPVIRGVA 289
+ +L+RDI L++++ +GD YT+GD FD + G ++ +DD MLP +APVI G+A
Sbjct: 246 INTWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA 305

Query: 290 NSNAKVTVMQSGNKIYETTVPPGAFEINDLSTTGYGNDLLVTVEEADGSKRSFTVPFSSV 349
A+VT+ Q+G IY +TVPPG F IND+ G DL VT++EADGS + FTVP+SSV
Sbjct: 306 RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSV 365

Query: 350 TQMLRPGATRWDIGVGEL-NDDSLHDKPQVGYAQFYYGLNNTFTGYIGAQYTDMNFYAGL 408
+ R G TR+ I GE + ++ +KP+ + +GL +T Y G Q D + A
Sbjct: 366 PLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLAD-RYRAFN 424

Query: 409 LGLAMNTG-IGAFAFDVTQSHASIDDLGTLSGQNYRLTYSKMIEATNTSFNVAAYRFSTE 467
G+ N G +GA + D+TQ+++++ D GQ+ R Y+K + + T+ + YR+ST
Sbjct: 425 FGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTS 484

Query: 468 DYLSLNDAASLQDSVKH---QQYAQQSYRSGDELYDDYQRTKNQVQISINQPLNQGETTW 524
Y + D + + + Q Q + Y+ + ++Q+++ Q L +
Sbjct: 485 GYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGR----T 540

Query: 525 GSVYVSGTWQDYWNDAGSTANYSVGYNNSFAYGSYSVSLQRAYDQNGSK-DDSVYLSFSI 583
++Y+SG+ Q YW + + G N +F ++++S + D + L+ +I
Sbjct: 541 STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600

Query: 584 PLSMFSHNGERSG-GFSNINMGLRSDMKGGTNVNSTASGNT-KDSDISYSVSA-TSSSGN 640
P S + + +S ++ + + D+ G + G +D+++SYSV + G+
Sbjct: 601 PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD 660

Query: 641 YGNLNQVSGFGSLNSSYGPLGLSASFGDDNSQQYSASYSGGMVLHSGGVAFTPGSIGETD 700
+ + + YG + S DD +Q SGG++ H+ GV + +T
Sbjct: 661 GNSGSTGYATLNYRGGYGNANIGYSHSDDI-KQLYYGVSGGVLAHANGVTLGQ-PLNDT- 717

Query: 701 AVALVKASGAQGAGL-GYSSSEIGSSGYGILPYMSAYRENRVSLDISTLENDVEIKSTST 759
V LVKA GA+ A + + GY +LPY + YRENRV+LD +TL ++V++ +
Sbjct: 718 -VVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVA 776

Query: 760 VTVPRSGAVVLVNFETDEGRSLILELLRSDKGFIPLGADVLNEKNETVGTVGQAGQAYVR 819
VP GA+V F+ G L++ L ++K +P GA V +E +++ G V GQ Y+
Sbjct: 777 NVVPTRGAIVRAEFKARVGIKLLMTLTHNNKP-LPFGAMVTSESSQSSGIVADNGQVYLS 835

Query: 820 GVEPQGELRVVWGSGKESTCTVRYQLAETTAKAGLTPVL 858
G+ G+++V WG + + C YQL + + LT +
Sbjct: 836 GMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLS 874


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0199FERRIBNDNGPP5010.0 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 501 bits (1292), Expect = 0.0
Identities = 246/296 (83%), Positives = 268/296 (90%)

Query: 1 MRELYPLTRRRLLTAMALSPLLWQMNTAQAAAIDPRRIVALEWLPVELLLALGITPYGVA 60
M L ++RRRLLTAMALSPLLWQMNTA AAAIDP RIVALEWLPVELLLALGI PYGVA
Sbjct: 1 MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVA 60

Query: 61 DVPNYKLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEKLARIAPGR 120
D NY+LWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPE LARIAPGR
Sbjct: 61 DTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGR 120

Query: 121 GFDFSDGKKPLAVARRSLVELAQTLNLEAAAEKHLAQYDRFIASQKPRFIRRGGRPLLMT 180
GF+FSDGK+PLA+AR+SL E+A LNL++AAE HLAQY+ FI S KPRF++RG RPLL+T
Sbjct: 121 GFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLT 180

Query: 181 TLIDPRHMLVLGPNCLFQEVLDEYGIVNAWQGETNFWGSTAVSIDRLAMYKEADVICFDH 240
TLIDPRHMLV GPN LFQE+LDEYGI NAWQGETNFWGSTAVSIDRLA YK+ DV+CFDH
Sbjct: 181 TLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDH 240

Query: 241 GNNTDMNALMATPLWQAMPFVRAGRFHRVPAVWFYGATLSTMHFVRILNNVLGGKA 296
N+ DM+ALMATPLWQAMPFVRAGRF RVPAVWFYGATLS MHFVR+L+N +GGKA
Sbjct: 241 DNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVLDNAIGGKA 296


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0204FIMBRIALPAPE324e-04 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 32.3 bits (73), Expect = 4e-04
Identities = 49/170 (28%), Positives = 70/170 (41%), Gaps = 25/170 (14%)

Query: 16 MLSAVSMPL---AAESKTVNMTLTIVVNAAPPCTVTGGEVEFGNV-LTTKVDGVNYRQAV 71
ML AV M AA++ T L I P CTV EV +G++ + V ++
Sbjct: 12 MLGAVLMSQHVHAADNLTFKGKLII-----PACTVQNAEVNWGDIEIQNLVQSGGNQKDF 66

Query: 72 GYRLSCNGRVSDYLKLQIQGNAVTINGESVLQTDV---DGLGIRLQTATDGALVSPGNTQ 128
++C + +K+ I N T N V T DGL I L + + + GN
Sbjct: 67 TVDMNCPYSLGT-MKVTITSNGQTGNSILVPNTSTASGDGLLIYLYNSNNSGI---GNAV 122

Query: 129 WLSFQYS----GGSGPA-----IEAIPVKDNGVTLTGGAFNAGATLVVDY 169
L Q + G+ PA + K N +L G F+A ATLV Y
Sbjct: 123 TLGSQVTPGKITGTAPARKITLYAKLGYKGNMQSLQAGTFSATATLVASY 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0206FIMBRIALPAPE333e-04 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 32.7 bits (74), Expect = 3e-04
Identities = 46/161 (28%), Positives = 72/161 (44%), Gaps = 18/161 (11%)

Query: 23 VSAADNLHFSGSLVASPCTLTMQGADIAEVDFSSLDASDFIPGGQSARKPVVFELTDCDS 82
V AADNL F G L+ CT+ AEV++ ++ + + G + + V +C
Sbjct: 22 VHAADNLTFKGKLIIPACTVQN-----AEVNWGDIEIQNLVQSGGNQKDFTV--DMNCPY 74

Query: 83 ALSNGVQVIFTGTEATGMRGILAIDSYSGASGIGIGIETLSGVPVGINNES--GAVFT-- 138
+L ++V T TG IL ++ S ASG G+ I + GI N G+ T
Sbjct: 75 SLGT-MKVTITSNGQTG-NSILVPNT-STASGDGLLIYLYNSNNSGIGNAVTLGSQVTPG 131

Query: 139 LVTGKN---TLSLNAWV-QRLPGEDLIPGRFSASALATFEY 175
+TG ++L A + + + L G FSA+A Y
Sbjct: 132 KITGTAPARKITLYAKLGYKGNMQSLQAGTFSATATLVASY 172


6SPA0286SPA0300Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0286-112-3.232351DNA repair protein RecO
SPA0287016-4.404134pyridoxal phosphate biosynthetic protein
SPA0288-117-4.344157holo-(acyl-carrier-protein) synthase
SPA0289-117-3.382285ferredoxin
SPA0290116-1.922740transcriptional regulator
SPA0291017-0.053389transmembrane transport protein
SPA0292-1151.016618oxidoreductase
SPA02930141.653178transcriptional regulator
SPA0294-2123.389186phophosugar binding protein
SPA0295-3122.940523PTS system transporter subunit IIBC
SPA0297-3123.145242hypothetical protein
SPA0298-2122.990641hypothetical protein
SPA0299-2133.514342hypothetical protein
SPA0300-2133.682319phosphoribosylformylglycineamide synthetase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0291TCRTETB330.002 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 33.3 bits (76), Expect = 0.002
Identities = 33/177 (18%), Positives = 69/177 (38%), Gaps = 3/177 (1%)

Query: 213 FWLLFMILALGVFSGMVISSSSAQIGMTQYGLLSGAL-VVSLVSIFNSIGRLFWGGLTDK 271
WL + V + MV++ S I + V + + SIG +G L+D+
Sbjct: 17 IWLCILSF-FSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 272 LGGYNTLVIVYLFTCVCMLLLLFFNGNTSVFYFSALGVGFAYAGILVIFPGLTSQNFGMR 331
LG L+ + C ++ + S+ + G A + + ++
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 332 NQGLNYGFMYFGFAVGAVIAPYVTSAIAKYTGSYNTVFILTTVLLLIGVVLTLITKK 388
N+G +G + A+G + P + IA Y ++ + ++ + ++ L + KK
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIH-WSYLLLIPMITIITVPFLMKLLKK 191


7SPA0324SPA0362Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA03242172.460493nifU-like protein
SPA0325-1163.131327hypothetical protein
SPA03260153.111196chaperone protein HscB
SPA03270141.757486chaperone protein HscA
SPA0328-1140.752609ferredoxin
SPA03290153.347788hypothetical protein
SPA03300134.442389peptidase B
SPA03310134.374049SseB protein
SPA03330144.826301hypothetical protein
SPA0334-1145.802757thiosulfate sulfurtransferase
SPA03350145.624216lipoprotein
SPA03360154.572892penicillin-binding protein 1C
SPA03382152.933377anaerobic reductase subunit
SPA03391162.835421anaerobic reductase subunit
SPA03401152.215834polyferredoxin
SPA03411161.433760nucleoside diphosphate kinase
SPA03420141.397194hypothetical protein
SPA03430151.898324DNA-binding protein
SPA03440161.247823GcpE protein (protein E)
SPA03450141.083140histidyl-tRNA synthetase
SPA03460153.363164hypothetical protein
SPA03470163.755723lipoprotein
SPA03480174.096888GTP-binding protein
SPA03490184.277814hypothetical protein
SPA03511194.355992hypothetical protein
SPA03520173.818270hypothetical protein
SPA03553191.433696exodeoxyribonuclease large subunit
SPA0356323-0.304913inosine-5'-monophosphate dehydrogenase
SPA0357322-2.546248GMP synthase
SPA0358539-7.772984hypothetical protein
SPA0359328-7.112750hypothetical protein
SPA0360320-4.561853hypothetical protein
SPA0361013-4.327145outer membrane lipoprotein
SPA0362012-3.573806hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0327SHAPEPROTEIN1205e-32 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 120 bits (303), Expect = 5e-32
Identities = 85/368 (23%), Positives = 148/368 (40%), Gaps = 68/368 (18%)

Query: 23 GIDLGTTNSLVATVRSGQAETLPDHEGRHLLPSVVHYQQQGHTVGYAARDNAAQDTANTI 82
IDLGT N+L+ G + +E PSVV A + ++
Sbjct: 14 SIDLGTANTLIYVKGQG----IVLNE-----PSVV------------AIRQDRAGSPKSV 52

Query: 83 SSV----KRMMGRSLADIQARYPHLPYRFKASVNGLPMIDTAAGLLNPVRVSADILKALA 138
++V K+M+GR+ +I A P M D G++ V+ +L+
Sbjct: 53 AAVGHDAKQMLGRTPGNIAAIRP--------------MKD---GVIADFFVTEKMLQHFI 95

Query: 139 ARA-SESLSGELDGVVITVPAYFDDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDS 197
+ S S V++ VP +R+ +++A+ AG + L+ EP AAAI GL
Sbjct: 96 KQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPV 155

Query: 198 GKEGVIAVYDLGGGTFDISILRLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAG--I 255
+ V D+GGGT +++++ L+ V +GGD FD + +Y+R G I
Sbjct: 156 SEATGSMVVDIGGGTTEVAVISLNGVV-----YSSSVRIGGDRFDEAIINYVRRNYGSLI 210

Query: 256 ADRSDNRVQRELLDAAITAKIALSDADTVRVNVAG---WQG-----EITREQFNDLISAL 307
+ + R++ E+ A + + V G +G + + + +
Sbjct: 211 GEATAERIKHEI-------GSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEP 263

Query: 308 VKRTLLACRRALKDAGVE-PQDVLE--VVMVGGSTRVPLVRERVGEFFGRTPLTAIDPDK 364
+ + A AL+ E D+ E +V+ GG + + + E G + A DP
Sbjct: 264 LTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLT 323

Query: 365 VVAIGAAI 372
VA G
Sbjct: 324 CVARGGGK 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0360PYOCINKILLER290.008 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 29.4 bits (65), Expect = 0.008
Identities = 19/68 (27%), Positives = 26/68 (38%), Gaps = 2/68 (2%)

Query: 3 KVILGAVLFTLSGSVLSSSLQDQLAAVAQAEQQGKNEENRQRDALQAKRDQEA--QQERQ 60
LFT + S L + AA A E N+ Q A ++ +E QQ
Sbjct: 185 TAAYNVKLFTEAISSLQIRMNTLTAAKASIEAAAANKAREQAAAEAKRKAEEQARQQAAI 244

Query: 61 RQANAAAV 68
R AN A+
Sbjct: 245 RAANTYAM 252



Score = 27.8 bits (61), Expect = 0.023
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%)

Query: 46 ALQAKRDQEAQQERQRQANAAAVAKQRAKAAEAERKARQAKLAAEA 91
+LQ + + + +A AA A+++A AAEA+RKA + A
Sbjct: 199 SLQIRMNTLTAAKASIEAAAANKAREQA-AAEAKRKAEEQARQQAA 243


8SPA0373SPA0384Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA03732150.877874hypothetical protein
SPA03751160.068639hypothetical protein
SPA03760121.671584permease
SPA0377-2120.819575hypothetical protein
SPA03780110.201201bacterioferritin comigratory protein
SPA0379-1103.564362glycine cleavage system transcriptional
SPA0380-1124.095516dihydrodipicolinate synthase
SPA0381-2103.993681lipoprotein
SPA0382-1113.593808phosphoribosylaminoimidazolesuccinocarboxamide
SPA0383-293.399215hypothetical protein
SPA0384-2103.795032hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0373SYCDCHAPRONE384e-05 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 37.6 bits (87), Expect = 4e-05
Identities = 31/149 (20%), Positives = 53/149 (35%), Gaps = 28/149 (18%)

Query: 290 NQLTSDLLDQWSKGNVHQQHAAQYGRALQAMEASKYDEARKTLQPLLSAEPNNAWYLDLA 349
N+++SD L+Q Y A ++ KY++A K Q L + ++ +
Sbjct: 29 NEISSDTLEQL------------YSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGL 76

Query: 350 TDIDLGQKRANDAINRLKNARDLRVN-PVLQLNLANAYLQGGQPKAAETILNRYTFSHKD 408
+ + AI+ + + P + A LQ G+ AE+ L
Sbjct: 77 GACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLF-------- 128

Query: 409 DGNGWDLLAQAEAALNNRDQELAARAESY 437
LAQ A +EL+ R S
Sbjct: 129 -------LAQELIADKTEFKELSTRVSSM 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0375HTHFIS320.003 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 32.5 bits (74), Expect = 0.003
Identities = 9/70 (12%), Positives = 26/70 (37%), Gaps = 6/70 (8%)

Query: 262 DHALPALLSGLSESWQVQELSRLWLQLVQHDAKGVLQQTLRTWFEHNCDLTQTAKALHIH 321
+ + + ++ L L +++ ++ L + + A L ++
Sbjct: 409 EENMRQYFASFGDALPPSGLYDRVLAEMEYP---LILAALT---ATRGNQIKAADLLGLN 462

Query: 322 VNTLRYRLQR 331
NTLR +++
Sbjct: 463 RNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0384AUTOINDCRSYN310.009 Autoinducer synthesis protein signature.
		>AUTOINDCRSYN#Autoinducer synthesis protein signature.

Length = 216

Score = 31.4 bits (71), Expect = 0.009
Identities = 24/122 (19%), Positives = 49/122 (40%), Gaps = 13/122 (10%)

Query: 459 SRIAVHPARQREGIGQQLIACACMQAAQCDYLSVSFGYT-------PELWRFWQRCGFVL 511
SR V +R ++ +G + + + + +Y S GY + +R G+
Sbjct: 100 SRFFVDKSRAKDILGNEYPISSMLFLSMINY-SKDKGYDGIYTIVSHPMLTILKRSGWG- 157

Query: 512 VRMGNHREASSGCYTAMALLPLSDAG-KRLAQQEHRRLRRDADILTQWNGEAIPLAALDE 570
+R+ + + LP+ D + LA++ +R ++ L QW + + A
Sbjct: 158 IRVVEQGLSEKEERVYLVFLPVDDENQEALARRINRSGTFMSNELKQW---PLRVPAAIA 214

Query: 571 QA 572
QA
Sbjct: 215 QA 216


9SPA0397SPA0415Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA03970163.602042NADP-dependent malate dehydrogenase
SPA03981204.487174ethanolamine utilization protein EutS
SPA03991205.122381ethanolamine utilization protein
SPA04002226.233493ethanolamine utilization protein
SPA04012227.011683cobalamin adenosyltransferase
SPA04024227.840363phosphate acyltransferase
SPA04033236.792308ethanolamine utilization protein EutN
SPA04042216.964928ethanolamine utilization protein EutN
SPA04051256.444296aldehyde dehydrogenase
SPA04061256.566295ethanolamine utilization protein EutJ
SPA04071246.259369alchohol dehydrogenase
SPA04080235.767968hypothetical protein
SPA0409-1185.203690ethanolamine utilization protein EutA
SPA0410-1164.275649ethanolamine ammonia-lyase heavy chain
SPA04110143.287880ethanolamine ammonia-lyase light chain
SPA04120142.110338ethanolamine utilization protein EutL
SPA04131131.434974ethanolamine utilization protein EutK
SPA04141110.775143ethanolamine operon transcriptional regulator
SPA04152131.204899coproporphyrinogen III oxidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0406SHAPEPROTEIN499e-09 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 48.6 bits (116), Expect = 9e-09
Identities = 32/116 (27%), Positives = 51/116 (43%), Gaps = 9/116 (7%)

Query: 64 VRDGIVWDFFGAVTLVRRHLDTLEQQLGCRFT-HAATSFPPGTDP---RISINVLESAGL 119
++DG++ DFF +++ + + R + P G R + AG
Sbjct: 76 MKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGA 135

Query: 120 EVSHVLDEPTAVA---DLLALDNAG--VVDIGGGTTGIAIVKQGKVTYSADEATGG 170
+++EP A A L + G VVDIGGGTT +A++ V YS+ GG
Sbjct: 136 REVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGG 191


10SPA0424SPA0468Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0424-3153.123291sulfate transport system permease CysW
SPA0425-2153.356253sulfate transport ATP-binding protein CysA
SPA0426-1162.962556cysteine synthase B
SPA0427-2141.599365hypothetical protein
SPA0428-1140.936775GMP synthase
SPA04291150.926749transcriptional regulator
SPA0430219-0.531866pyridoxine kinase
SPA0431223-1.147702hypothetical protein
SPA0432122-0.362659pts system, glucose-specific IIA component
SPA04330151.256007hypothetical protein
SPA0434-1142.122886phosphocarrier protein HPr
SPA0435-1152.237801cysteine synthase A
SPA04360142.677405sulfate transport protein CysZ
SPA04370162.837137cell division protein
SPA04380162.048137DNA ligase
SPA04392150.983349hypothetical protein
SPA0440011-0.149999hypothetical protein
SPA0441-110-0.170000transcriptional regulator
SPA0446-211-0.588904****glutamyl-tRNA synthetase
SPA0447-212-0.914154hypothetical protein
SPA0448-1111.051469hypothetical protein
SPA0450-1111.979497*hypothetical protein
SPA04521123.153180manganese transport protein MntH
SPA04531133.039402hypothetical protein
SPA04541132.853672ion-channel protein
SPA04551132.645842decarboxylase
SPA04561130.264346hypothetical protein
SPA0457012-0.885557glucokinase
SPA0458-212-1.045648aminotransferase
SPA0459-115-2.309747acyltransferase
SPA0460230-7.721885hypothetical protein
SPA0461130-8.131565phosphoglycerate transporter protein
SPA0462135-9.371880phosphoglycerate transport regulatory protein
SPA0464235-10.497801phosphoglycerate transport system
SPA0465335-11.095416outer membrane protease E
SPA0467230-9.348130lipopolysaccharide modification acyltransferase
SPA0468015-3.413203bactoprenol glucosyl transferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0425PF05272348e-04 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 34.3 bits (78), Expect = 8e-04
Identities = 12/33 (36%), Positives = 16/33 (48%)

Query: 30 MVALLGPSGSGKTTLLRIIAGLEHQSSGHIRFH 62
V L G G GK+TL+ + GL+ S H
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIG 630


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0433PHPHTRNFRASE7480.0 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 748 bits (1934), Expect = 0.0
Identities = 276/571 (48%), Positives = 388/571 (67%), Gaps = 2/571 (0%)

Query: 1 MISGILASPGIAFGKALLLKEDEIVIDRKKISADKVDQEVERFLSGRAKASAQLEAIKTK 60
I+GI AS G+A KA + E + I++ I V E+E+ + K+ +L AIK +
Sbjct: 4 KITGIAASSGVAIAKAFIHLEPNVDIEKTSI--TDVSTEIEKLTAALEKSKEELRAIKDQ 61

Query: 61 AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQATALEELDD 120
+ G +K IF H+++L+D EL I I+++ M A+ A EV + + E +D+
Sbjct: 62 TEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDN 121

Query: 121 EYLKERAADVRDIGKRLLRNILGLAIIDLSAIQEEVILVAADLTPSETAQLNLQKVLGFI 180
EY+KERAAD+RD+ KR+L +++G+ L+ I EE +++A DLTPS+TAQLN Q V GF
Sbjct: 122 EYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFA 181

Query: 181 TDAGGRTSHTSIMARSLELPAIVGTGSVTAQVKNGDYLILDAVNNQVYVNPTNDVIEQLR 240
TD GGRTSH++IM+RSLE+PA+VGT VT ++++GD +I+D + V VNPT + ++
Sbjct: 182 TDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYE 241

Query: 241 AVQEQVATEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFL 300
+ +K E AKL P+ T DG VE+ ANIGT +DV+G NG EG+GLYRTEFL
Sbjct: 242 EKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFL 301

Query: 301 FMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAV 360
+MDRD LPTEEEQF AYK V + + V++RT+DIGGDKEL Y+ PKE NPFLG+RA+
Sbjct: 302 YMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFRAI 361

Query: 361 RIAMDRKEILRDQVRAILRASAFGKLRIMFPMIISVEEVRALRKEIEIYKQELRDEGKAF 420
R+ +++++I R Q+RA+LRAS +G L++MFPMI ++EE+R + ++ K +L EG
Sbjct: 362 RLCLEKQDIFRTQLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDV 421

Query: 421 DESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS 480
+SIE+G+MVE P+ A A AKEVDFFSIGTNDL QYT+A DR N+ +S+LYQP P+
Sbjct: 422 SDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPA 481

Query: 481 VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT 540
+L L+ VI A+H+EGKW GMCGE+AGDE A LLLG+GLDEFSMSA SI + +
Sbjct: 482 ILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKL 541

Query: 541 NFEDAKVLAEQALAQPTTDELMTLVNKFIEE 571
+ E+ K A++AL T +E+ LV K +
Sbjct: 542 SKEELKPFAQKALMLDTAEEVEQLVKKTYLK 572


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0437PF03544421e-06 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 41.9 bits (98), Expect = 1e-06
Identities = 25/128 (19%), Positives = 39/128 (30%), Gaps = 11/128 (8%)

Query: 68 VHRVNHAPGQSQEHDAPRQSPQHQYQPPYASAQPRPAAPPQPQAPMQQPVQQPVQPAPQP 127
VH+V P +Q +P P P P P+P +P P P P
Sbjct: 37 VHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEP-----EPEPIPEPPKEAP 91

Query: 128 QQVQPSAPPVQPPQQQPAPPSQAPQPVAQPAPPPSAQTFQPAEPVVE-----AEPVVEEA 182
++ P P+ +P + P+ +P A F+ P +
Sbjct: 92 VVIEKPKPK-PKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPV 150

Query: 183 PVVEKPQR 190
V R
Sbjct: 151 TSVASGPR 158



Score = 38.0 bits (88), Expect = 2e-05
Identities = 18/82 (21%), Positives = 21/82 (25%)

Query: 96 YASAQPRPAAPPQPQAPMQQPVQQPVQPAPQPQQVQPSAPPVQPPQQQPAPPSQAPQPVA 155
Y S P Q V PQ Q P P+ +P P PV
Sbjct: 34 YTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVV 93

Query: 156 QPAPPPSAQTFQPAEPVVEAEP 177
P P + VE
Sbjct: 94 IEKPKPKPKPKPKPVKKVEQPK 115



Score = 35.0 bits (80), Expect = 2e-04
Identities = 26/79 (32%), Positives = 30/79 (37%), Gaps = 4/79 (5%)

Query: 117 VQQPVQPAP-QPQQVQPSAPPVQPPQQQPAPPSQAPQPVAQPAPPPSAQTFQPAEPVVEA 175
Q PAP QP V AP P Q PP P+PV +P P P P E V
Sbjct: 38 HQVIELPAPAQPISVTMVAPADLEPPQAVQPP---PEPVVEPEPEPEPIPEPPKEAPVVI 94

Query: 176 EPVVEEAPVVEKPQRKEAV 194
E + KP +K
Sbjct: 95 EKPKPKPKPKPKPVKKVEQ 113



Score = 28.0 bits (62), Expect = 0.042
Identities = 10/80 (12%), Positives = 23/80 (28%)

Query: 93 QPPYASAQPRPAAPPQPQAPMQQPVQQPVQPAPQPQQVQPSAPPVQPPQQQPAPPSQAPQ 152
+P P+P+ + V+QP + + S P + + + A
Sbjct: 87 PKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAAT 146

Query: 153 PVAQPAPPPSAQTFQPAEPV 172
+ + +P
Sbjct: 147 SKPVTSVASGPRALSRNQPQ 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0441RTXTOXINC290.010 Gram-negative bacterial RTX toxin-activating protein C...
		>RTXTOXINC#Gram-negative bacterial RTX toxin-activating protein C

signature.
Length = 170

Score = 29.5 bits (66), Expect = 0.010
Identities = 20/58 (34%), Positives = 29/58 (50%), Gaps = 14/58 (24%)

Query: 161 AILSEPFLLLCRQDHPLAHQEWVSWQDLKQ----------ASLVLQDYASGSRP-LID 207
AI + ++LL R D+P+A + SW +L SLV +D+ SG R ID
Sbjct: 38 AIQANQYVLLTRDDYPVA---YCSWANLSLENEIKYLNDVTSLVAEDWTSGDRKWFID 92


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0448BINARYTOXINA270.027 Clostridial binary toxin A signature.
		>BINARYTOXINA#Clostridial binary toxin A signature.

Length = 454

Score = 26.6 bits (58), Expect = 0.027
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 3/36 (8%)

Query: 5 RMTPEELANLTGYSR---QTINKWVRKEGWATSPKP 37
++TP ELA++ Y R IN ++ G +P P
Sbjct: 275 KLTPNELADVNDYMRGGYTAINNYLISNGPLNNPNP 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0461TCRTETA310.007 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 31.3 bits (71), Expect = 0.007
Identities = 40/217 (18%), Positives = 72/217 (33%), Gaps = 14/217 (6%)

Query: 28 IQALLSVFLGYLAYYIVRNNFTLSTPYLKEQLDLSATQI---GLLSSCMLIAYGISKGVM 84
I L +V L + ++ P L L S G+L + + V+
Sbjct: 8 IVILSTVALDAVGIGLI----MPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVL 63

Query: 85 SSLADKASPKVFMACGLVLCAIVNVGLGFSSAFWIFAALVVFNGLFQGMGVGPSFITIAN 144
+L+D+ + + L A+ + + W+ + G+ G IA+
Sbjct: 64 GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAG-AYIAD 122

Query: 145 WFPRRERGRVGAFWNISHNVGGGIVA-PIVGAAFAILGNEHWQSASYIVPACVAVIFALI 203
ER R F +S G G+VA P++G ++G + + A + F
Sbjct: 123 ITDGDERAR--HFGFMSACFGFGMVAGPVLG---GLMGGFSPHAPFFAAAALNGLNFLTG 177

Query: 204 VLVLGKGSPREEGLPSLEQMMPEEKVVLKTKNTAKAP 240
+L + E E + P T A
Sbjct: 178 CFLLPESHKGERRPLRREALNPLASFRWARGMTVVAA 214


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0464HTHFIS2486e-80 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 248 bits (635), Expect = 6e-80
Identities = 120/474 (25%), Positives = 191/474 (40%), Gaps = 73/474 (15%)

Query: 7 SILLIDDDVDVLDAYTQMLEQAGYRVRGFTHPFEAKEWVKADWEGIVLSDVCMPGCSGID 66
+IL+ DDD + Q L +AGY VR ++ W+ A +V++DV MP + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 67 LMTLFHQDDDQLPILLITGHGDVPMAVDAVKKGAWDFLQKPVDPGNLLILIEDALRQRRS 126
L+ + LP+L+++ A+ A +KGA+D+L KP D L+ +I AL + +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 127 VIARRQYCQQTLQVDLIGRSEWMNQFRQRLQQLAETDIAVWFYGEHGTGRMTGARYLHQL 186
++ + Q L+GRS M + + L +L +TD+ + GE GTG+ AR LH
Sbjct: 125 RPSKLEDDSQDGM-PLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY 183

Query: 187 GRNAKGPFVRYELT--PENAGQLETF-----------------IDQAQGGTLVLSHPEYL 227
G+ GPFV + P + + E F +QA+GGTL L +
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 228 TREQQHHLAR-LQSLEHRP----------FRLVGVGSASLVEQAAANQIAAELYYCFAMT 276
+ Q L R LQ E+ R+V + L + +LYY +
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 277 QIACQSLSQRPDDIEPLFRHYLRKACLRLNHPVPEIAGELLKGIMRRAWPSNVRELANAA 336
+ L R +DI L RH++++A + V E L+ + WP NVREL N
Sbjct: 304 PLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 337 ELFAV-----------------------------------GVLPLAETVNPQLL------ 355
+ E Q
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 356 LQEPTPLDRRVEEYERQIITEALNIHQGRINEVAEYLQIPRKKLYLRMKKYGLS 409
L DR + E E +I AL +G + A+ L + R L ++++ G+S
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0465OMPTIN472e-172 Omptin serine protease signature.
		>OMPTIN#Omptin serine protease signature.

Length = 317

Score = 472 bits (1217), Expect = e-172
Identities = 149/320 (46%), Positives = 211/320 (65%), Gaps = 11/320 (3%)

Query: 1 MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYD-TDTGRK 59
M+ + +++ + S +A + +P+++ +S+G L+GK++E VY + GRK
Sbjct: 1 MRAKLLGIVLTTPIAISSFASTET--LSFTPDNINADISLGTLSGKTKERVYLAEEGGRK 58

Query: 60 LSQLDWKIKNVATLQGDLSWKPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPG-WTDR 118
+SQLDWK N A ++G ++W +++ A GWT+L S G+MVD DWM S PG WTD
Sbjct: 59 VSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDE 118

Query: 119 SIHPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGR---- 174
S HPDT NYANE+DLN+KGWLL NY+ G+ AGYQE+R+S+TARGGSYIY +
Sbjct: 119 SRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRD 178

Query: 175 YIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHY--MRK 232
IG+FP+G R IGY QRF+MPYIGL G YR DFE FKYS WV + DNDEHY ++
Sbjct: 179 DIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKR 238

Query: 233 LTFREKTENSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGG 292
+T+R K ++ YY +++AGYY+T NAK++ E A+++ KG T + D + NT+ +
Sbjct: 239 ITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNN-NTSDYSK 297

Query: 293 DAAGIANNNYTVTAGLQYRF 312
+ AGI N N+ TAGL+Y F
Sbjct: 298 NGAGIENYNFITTAGLKYTF 317


11SPA0480SPA0495Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0480-1143.548537chorismate synthase
SPA0481-2123.192624penicillin-insensitive murein endopeptidase
SPA0482-2120.893999hypothetical protein
SPA0483-2130.569691hypothetical protein
SPA0484-2140.355406hypothetical protein
SPA0485-3140.108933hypothetical protein
SPA0486-121-2.6827983-oxoacyl-ACP synthase
SPA0487223-1.361272hypothetical protein
SPA0488-1181.552236lipoprotein
SPA0489-3142.471446hypothetical protein
SPA0490-2143.255774DNA-binding protein
SPA0491-2153.539707bacteriophage protein
SPA0492-1143.261133transmembrane transporter
SPA0493-1132.439735Div protein
SPA0494-1143.163015erythronate-4-phosphate dehydrogenase
SPA0495-1153.352834semialdehyde dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0492TCRTETA423e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 41.7 bits (98), Expect = 3e-06
Identities = 78/362 (21%), Positives = 133/362 (36%), Gaps = 30/362 (8%)

Query: 14 NFSLFRIAFAAFLTYMTVGLPLPVIPLFVHHELGYSNTMV---GIAVGIQFFATVLTRGY 70
N L I L + +GL +PV+P + +L +SN + GI + +
Sbjct: 4 NRPLIVILSTVALDAVGIGLIMPVLPGLLR-DLVHSNDVTAHYGILLALYALMQFACAPV 62

Query: 71 AGRLADQYGAKRSALQGMFACGLAGAAWLLAALLPVSAPVKFALLIVGRLILGFGESQLL 130
G L+D++G + + LAGAA + + +AP +L +GR++ G +
Sbjct: 63 LGALSDRFGRRP-----VLLVSLAGAA--VDYAIMATAPF-LWVLYIGRIVAGITGATGA 114

Query: 131 TGTLTWGLGLVGPTRSGKVMSWNGMAIYGALAAGAPLGLL---IHSHFGFAALAGTTMVL 187
G R+ + + + AG LG L H F A A +
Sbjct: 115 VAGAYIADITDGDERA-RHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLN 173

Query: 188 PLLAWAFNGTVRKVPAYTGERPSLWSVVGLIWKPGL-----------GLALQGVGFAVIG 236
L K R +L + W G+ + L G A +
Sbjct: 174 FLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALW 233

Query: 237 TFISLYFVSNGWTMAGFTLTAFGGAFVLMRIL-FGWMPDRFGGVKVAVVSLLVETAGLLL 295
T G +L AFG L + + G + R G + ++ ++ + G +L
Sbjct: 234 VIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYIL 293

Query: 296 LWLAPTAWIALVGAALTGAGCSLIFPALGVEVVKRVPAQVRGTALGGYAAFQDISYGVTG 355
L A W+A L +G + PAL + ++V + +G G AA ++ + G
Sbjct: 294 LAFATRGWMAFPIMVLLASG-GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT-SIVG 351

Query: 356 PL 357
PL
Sbjct: 352 PL 353


12SPA0536SPA0558Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0536-2264.108797NADH dehydrogenase I subunit A
SPA0537-1274.035238NADH dehydrogenase I subunit B
SPA0538-1294.084342NADH dehydrogenase I subunit CD
SPA0539-1294.248845NADH dehydrogenase I subunit E
SPA0540-1294.187921NADH dehydrogenase I subunit F
SPA05411294.057723NADH dehydrogenase I subunit G
SPA05422302.975561NADH dehydrogenase I subunit H
SPA05433313.999952NADH dehydrogenase I subunit I
SPA05442293.400929NADH dehydrogenase I subunit J
SPA05451243.211122NADH dehydrogenase I subunit K
SPA05461233.126517NADH dehydrogenase I subunit L
SPA05470182.350521NADH dehydrogenase I subunit M
SPA0548-1122.547228NADH dehydrogenase I subunit N
SPA0549-1132.746290receptor/regulator protein
SPA0550-1133.983417hydrolase
SPA0551-1134.084342hypothetical protein
SPA0552-1124.804456hypothetical protein
SPA05530125.567396isochorismate synthase
SPA05540135.579170menaquinone biosynthesis protein
SPA05550134.861791hypothetical protein
SPA05561134.700499naphthoate synthase
SPA0557-1153.708240O-succinylbenzoate-CoA synthase
SPA05580153.072974O-succinylbenzoic acid--CoA ligase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0537FLGBIOSNFLIP280.019 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 28.3 bits (63), Expect = 0.019
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 3/56 (5%)

Query: 68 MVTSFT---AVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEP 120
M+TSFT V + R A P Q L + F M+PVI ++Y +P
Sbjct: 60 MMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQP 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0549HTHFIS462e-07 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 45.6 bits (108), Expect = 2e-07
Identities = 32/148 (21%), Positives = 58/148 (39%), Gaps = 16/148 (10%)

Query: 185 PGAVAIVAEDSKVARAMLEKGLNAMEIPHQMHVTGKDAWERIQQLAQEAEAEGKPISEKI 244
GA +VA+D R +L + L+ ++ W I +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIA-------------AGDG 48

Query: 245 ALVLTDLEMPEMDGFTLTRKIKTDERLKKIPVVIHSSLSGSANEDHVRKVKADGYVAK-F 303
LV+TD+ MP+ + F L +IK + +PV++ S+ + + A Y+ K F
Sbjct: 49 DLVVTDVVMPDENAFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106

Query: 304 EINELSSVIQEVMERAAQNISGPLVSRQ 331
++ EL +I + + S Q
Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLEDDSQ 134


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0551AUTOINDCRSYN300.002 Autoinducer synthesis protein signature.
		>AUTOINDCRSYN#Autoinducer synthesis protein signature.

Length = 216

Score = 30.2 bits (68), Expect = 0.002
Identities = 11/74 (14%), Positives = 27/74 (36%), Gaps = 12/74 (16%)

Query: 1 MIDWQDLHHSELTVPQLYALLKLRCAVFV--------VEQRCPYLDVDGDDLVGDNRHIL 52
M++ D++H+ L+ + L LR F + D + + ++
Sbjct: 1 MLEIFDVNHTLLSETKSGELFTLRKETFKDRLNWAVQCTDGMEFDQYDNN----NTTYLF 56

Query: 53 GWHQDELVAYARIL 66
G + ++ R +
Sbjct: 57 GIKDNTVICSLRFI 70


13SPA0585SPA0619Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0585-115-3.884030ferredoxin
SPA0586-113-5.078652ribonucleoside-diphosphate reductase 1 subunit
SPA0587-113-3.266714ribonucleoside-diphosphate reductase 1 subunit
SPA0588-29-2.9916263-demethylubiquinone-9 3-methyltransferase
SPA0589-19-3.426570hypothetical protein
SPA0590-19-2.383113hypothetical protein
SPA0591013-1.096877MR-MLE-family protein
SPA05920140.307383DNA gyrase subunit A
SPA0593-2120.501652sensor protein RcsC
SPA0594-2131.025684regulator of capsule synthesis B component
SPA0595-3111.450725two-component system sensor kinase
SPA0597-2152.959943outer membrane protein C
SPA0598-1143.613631thiamine biosynthesis protein
SPA0599-2143.473471ADA regulatory protein
SPA0600-2152.795332AlkB protein
SPA0601-1162.922495ABC transporter ATP-binding protein
SPA06020143.085497ecotin precursor
SPA06030173.302298ferredoxin-type protein NapF
SPA06040182.782784napAB assembly protein
SPA06050214.691342nitrate reductase
SPA06061297.423357ferredoxin-type protein NapG
SPA06072348.400744ferredoxin-type protein NapH
SPA060834110.986420cytochrome c-type protein NapB precursor
SPA060944712.125596cytochrome c-type protein NapC
SPA061085615.029081heme exporter protein A1
SPA061175313.631854heme exporter protein B1
SPA061265313.404905heme exporter protein C1
SPA061344711.724712heme exporter protein D1
SPA061434310.236964cytochrome c-type biogenesis protein E1
SPA06152388.957309cytochrome c-type biogenesis protein F1
SPA06160202.669705thiol:disulfide interchange protein
SPA06171161.678536cytochrome c-type biogenesis protein H1
SPA0618011-1.829620nitrate/nitrite response regulator protein NarP
SPA0619014-3.105303hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0589NUCEPIMERASE280.031 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 28.2 bits (63), Expect = 0.031
Identities = 16/75 (21%), Positives = 29/75 (38%), Gaps = 15/75 (20%)

Query: 133 AERDTQAYLKLDHDFHYVFVKYADNKYISQAHLLISARLLAIRYRLDFTAEYITSSNRGH 192
A+R+ L F VF+ + A+RY L+ Y S+ G
Sbjct: 62 ADREGMTDLFASGHFERVFISPH------RL---------AVRYSLENPHAYADSNLTGF 106

Query: 193 ATILDMLKNNNVEGV 207
IL+ ++N ++ +
Sbjct: 107 LNILEGCRHNKIQHL 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0590TCRTETB310.012 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 30.6 bits (69), Expect = 0.012
Identities = 36/179 (20%), Positives = 68/179 (37%), Gaps = 12/179 (6%)

Query: 25 ILYFFNYMDRVNIGFAALRMNESLGITPEDFANISSIFFISYLIFQIPSSIGLQKLGARK 84
IL FF+ ++ + + + + P +++ F +++ I +LG ++
Sbjct: 21 ILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKR 80

Query: 85 W--ISSIIIGWGAVTGLIFFAKDTQHIL-LARIFLGVFEAGFFPGMVYYLACWFPARERG 141
II +G+V G F +L +AR G A F ++ +A + P RG
Sbjct: 81 LLLFGIIINCFGSVIG--FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138

Query: 142 KVNSFFMLSIAVASVLAAPMSGWIIEHLNTPDYEGWRWLFAIEGIPTVFLGILTFYLLP 200
K +A+ + + G I ++ W +L I I T+ LL
Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYI------HWSYLLLIPMI-TIITVPFLMKLLK 190


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0593HTHFIS801e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 79.9 bits (197), Expect = 1e-17
Identities = 29/104 (27%), Positives = 47/104 (45%)

Query: 827 ILVVDDHPINRRLLADQLGSLGYQCKTANDGVDALNVLSKNAIDIVLSDVNMPNMDGYRL 886
ILV DD R +L L GY + ++ ++ D+V++DV MP+ + + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 887 TQRIRQLGLTLPVVGVTANALAEEKQRCLESGMDSCLSKPVTLD 930
RI++ LPV+ ++A + E G L KP L
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0594HTHFIS488e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 47.9 bits (114), Expect = 8e-09
Identities = 26/145 (17%), Positives = 60/145 (41%), Gaps = 20/145 (13%)

Query: 1 MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLPKLDAHVLITDLSMP 60
M +++ADD + + ++L + + + ++ L + D +++TD+ MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRI--TSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 GDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGA------PT 114
+ L+ IK+ P L ++V++ N +A+ ++GA P
Sbjct: 59 D---ENAFDLLPRIKKARPDLPVLVMSAQNTFM--TAIKA-------SEKGAYDYLPKPF 106

Query: 115 DLPKALAALQKGKKFTPESVSRLLE 139
DL + + + + S+L +
Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0597ECOLIPORIN5400.0 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 540 bits (1392), Expect = 0.0
Identities = 261/389 (67%), Positives = 298/389 (76%), Gaps = 17/389 (4%)

Query: 1 MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLFGKVDGLHYFSDDKGSDGDQTYMRIG 60
MK KVL+L++PALL AGAA+AAEIYNKDGNKLDL+GKVDGLHYFSDD DGDQTYMR+G
Sbjct: 1 MKRKVLALVIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDDSSKDGDQTYMRVG 60

Query: 61 FKGETQVNDQLTGYGQWEYQIQGNQTEG-SNDSWTRVAFAGLKFADAGSFDYGRNYGVTY 119
FKGETQ+NDQLTGYGQWEY +Q N TEG +SWTR+AFAGLKF D GSFDYGRNYGV Y
Sbjct: 61 FKGETQINDQLTGYGQWEYNVQANTTEGEGANSWTRLAFAGLKFGDYGSFDYGRNYGVLY 120

Query: 120 DVTSWTDVLPEFGGDTYG-ADNFMQQRGNGYATYRNTDFFGLVDGLDFALQYQGKNGSVS 178
DV WTD+LPEFGGD+Y ADN+M R NG ATYRNTDFFGLVDGL+FALQYQGKN S S
Sbjct: 121 DVEGWTDMLPEFGGDSYTYADNYMTGRANGVATYRNTDFFGLVDGLNFALQYQGKNESQS 180

Query: 179 GEN--------TNGRSLLNQNGDGYGGSLTYAIGEGFSVGGAITTSKRTADQNNTANARL 230
++ NG + NGDG+G S TY IG GFS G A TTS RT +Q N
Sbjct: 181 ADDVNIGTNNRNNGDDIRYDNGDGFGISTTYDIGMGFSAGAAYTTSDRTNEQVNAGG--T 238

Query: 231 YGNGDRATVYTGGLKYDANNIYLAAQYSQTYNATRFGTSNGSNPSTSYGFANKAQNFEVV 290
GD+A +T GLKYDANNIYLA YS+T N T +G ++ G ANK QNFEV
Sbjct: 239 IAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPYGKTDK---GYDGGVANKTQNFEVT 295

Query: 291 AQYQFDFGLRPSVAYLQSKGKDISNGYGASYGDQDIVKYVDVGATYYFNKNMSTYVDYKI 350
AQYQFDFGLRP+V++L SKGKD++ + D+D+VKY DVGATYYFNKN STYVDYKI
Sbjct: 296 AQYQFDFGLRPAVSFLMSKGKDLTYN-NVNGDDKDLVKYADVGATYYFNKNFSTYVDYKI 354

Query: 351 NLLDKND-FTRDAGINTDDIVALGLVYQF 378
NLLD +D F +DAGI+TDDIVALG+VYQF
Sbjct: 355 NLLDDDDPFYKDAGISTDDIVALGMVYQF 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0614PF04335290.006 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 29.0 bits (65), Expect = 0.006
Identities = 10/30 (33%), Positives = 12/30 (40%)

Query: 1 MNLRRKNRLWVVCAVLAGLALTTALVLYAL 30
R K WVV V LA + + AL
Sbjct: 27 AAERSKKLAWVVAGVAGALATAGVVAVAAL 56


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0618HTHFIS682e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.9 bits (166), Expect = 2e-15
Identities = 24/114 (21%), Positives = 48/114 (42%), Gaps = 2/114 (1%)

Query: 9 VLIVDDHPLMRRGIRQLLELDPAFYVVAEAGDGASAIDLANRIEPDLILLDLNMKGLSGL 68
+L+ DD +R + Q L A Y V + A+ + DL++ D+ M +
Sbjct: 6 ILVADDDAAIRTVLNQALSR--AGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 69 DTLNALRRDGVTAQIIILTVSDSASDIYALIDAGADGYLLKDSDPEVLLEAIRK 122
D L +++ +++++ ++ + GA YL K D L+ I +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


14SPA0661SPA0738Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0661215-2.445709D-galactose-binding periplasmic protein
SPA0663315-1.695800galactoside transport system permease protein
SPA0664418-1.103761dihydropyrimidine dehydrogenase
SPA0665420-1.356736oxidoreductase
SPA0666218-0.262597hypothetical protein
SPA06672171.911285vancomycin resistance protein
SPA06681162.596670cytidine deaminase
SPA0669-1142.310666hypothetical protein
SPA0670-2142.358040hypothetical protein
SPA0671-1153.709536transcriptional regulator
SPA06720164.064792n-hydroxybenzoate transporter
SPA06730182.977652gentisate 1,2-dioxygenase
SPA06740173.294424FAA-hydrolase-family protein
SPA06751183.236567glutathione-S-transferase-family protein
SPA06762183.434880n-hydroxybenzoate hydroxylase
SPA06771172.443771hypothetical protein
SPA06782141.758946hypothetical protein
SPA06791141.857721lipoprotein
SPA06800141.101690oxidoreductase
SPA0681-1131.215047hypothetical protein
SPA0682-2131.740639hypothetical protein
SPA0683-1122.035189penicillin-binding protein 7
SPA0684-2132.594489D-lactate dehydrogenase
SPA0685-2153.179665periplasmic beta-glucosidase
SPA0686-2173.842394hypothetical protein
SPA0687-1172.813128permease
SPA0688-2161.184833ABC transporter ATP-binding protein
SPA0689-2170.213781permease
SPA0690-213-2.361289hypothetical protein
SPA0691-213-3.188403transcriptional regulator
SPA0692-111-2.006075two-component system sensor kinase
SPA0693-111-1.483474two-component system response regulator
SPA0694-213-1.581140hypothetical protein
SPA0695-214-2.776608lipoprotein
SPA0696-213-3.244820lipoprotein
SPA0697-113-3.979751methionyl-tRNA synthetase
SPA0698227-7.179698hypothetical protein
SPA0699335-9.149765hypothetical protein
SPA0700129-7.028106fimbrial subunit protein
SPA0701123-4.550018fimbrial chaperone protein
SPA0702121-2.981654outer membrane usher protein
SPA07031190.981619hypothetical protein
SPA07041184.668621hypothetical protein
SPA07050143.821287hydroxyethylthiazole kinase
SPA07060142.660805phosphomethylpyrimidine kinase
SPA0707-1141.963126GntR family transcriptional regulator
SPA0708-1152.006297sugar kinase
SPA0709-115-0.843904hydrolase
SPA0710-116-2.691004nucleoside permease
SPA0711119-1.719275fructose-bisphosphate aldolase
SPA0712-118-2.721944diacylglycerol kinase catalytic domain
SPA0713022-4.486806hypothetical protein
SPA0714023-6.579970hypothetical protein
SPA0715021-5.785519hypothetical protein
SPA0716020-7.517285protease
SPA0717751-14.907606hypothetical protein
SPA07181059-18.995890hypothetical protein
SPA07191061-19.821320hypothetical protein
SPA07201161-20.763008hypothetical protein
SPA07211160-21.913834hypothetical protein
SPA07221061-21.249496hypothetical protein
SPA07231059-21.096370hypothetical protein
SPA0724961-21.258778hypothetical protein
SPA0725447-16.572777hypothetical protein
SPA0726125-8.735207hypothetical protein
SPA0727018-5.512691hypothetical protein
SPA0728016-3.947105hypothetical protein
SPA0729013-2.437493hypothetical protein
SPA0730-1140.973326hypothetical protein
SPA0731-2120.999485hypothetical protein
SPA0732-1142.148384hypothetical protein
SPA0733-2142.785807hypothetical protein
SPA0734-1153.752068two-component system response regulator
SPA0735-1164.259376two-component system sensor kinase
SPA0736-1163.499820transporter protein
SPA0737-1153.229573RND-family transporter protein
SPA07380153.368001RND-family transporter protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0672TCRTETB514e-09 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 51.4 bits (123), Expect = 4e-09
Identities = 64/425 (15%), Positives = 137/425 (32%), Gaps = 65/425 (15%)

Query: 22 RVIICCFLVVMLDGFDTAAIGFIAPDIRTHWQLSASELAPLFGAGLLGLTAGALLCGPLA 81
+++I ++ + + PDI + + + A +L + G + G L+
Sbjct: 14 QILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLS 73

Query: 82 DRFGRKRVIELCVALFGALSLLSAFS-PDIETLVLLRFLTGLGLGGAMPNTIT-MTSEYL 139
D+ G KR++ + + S++ L++ RF+ G G A P + + + Y+
Sbjct: 74 DQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAG-AAAFPALVMVVVARYI 132

Query: 140 PARRRGALVTLMFCGFTLGSAMGGIVSAQLVPLIGWHGILALGGILPLMLFFGLLFALPE 199
P RG L+ +G +G + + I W +L + I + + F + E
Sbjct: 133 PKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKE 192

Query: 200 SPRWQVRRQLPQAVVARTVSAITGERYHDTQFFLHEVAAVAKGSIRQLFAGRQLVITLML 259
R + LF + L++
Sbjct: 193 ------------------------VRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIV 228

Query: 260 WVVFFMSLLIIYLLSSWMPTLLNHRGIDLQQASWVTAAFQVGGTLGALLL---------- 309
V+ F+ + + ++ P + G ++ V + GT+ +
Sbjct: 229 SVLSFL-IFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVH 287

Query: 310 --------------------------GVLMDRLNPFRVLAVSYALGAVCIVMIGLSENG- 342
G+L+DR P VL + +V +
Sbjct: 288 QLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETT 347

Query: 343 LWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGAIVGSLSGGMM 402
W M + I G+S ++ ++ + ++ Q G+S N G G +
Sbjct: 348 SWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGL 407

Query: 403 MTLNF 407
+++
Sbjct: 408 LSIPL 412



Score = 43.3 bits (102), Expect = 2e-06
Identities = 40/169 (23%), Positives = 73/169 (43%), Gaps = 1/169 (0%)

Query: 251 RQLVITLMLWVVFFMSLLIIYLLSSWMPTLLNHRGIDLQQASWVTAAFQVGGTLGALLLG 310
R I + L ++ F S+L +L+ +P + N +WV AF + ++G + G
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 311 VLMDRLNPFRVLAVSYALGAVCIVMIGLSENGLWLMALAIFGTGIGISGSQVGLNALTAT 370
L D+L R+L + V+ + + L+ +A F G G + + + A
Sbjct: 71 KLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVAR 130

Query: 371 LYPTQSRATGVSWSNAIGRCGAIVGSLSGGMMM-TLNFSFDTLFFVIAI 418
P ++R +I G VG GGM+ +++S+ L +I I
Sbjct: 131 YIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITI 179


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0680DHBDHDRGNASE1152e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 115 bits (290), Expect = 2e-33
Identities = 69/253 (27%), Positives = 116/253 (45%), Gaps = 12/253 (4%)

Query: 3 KVAIVTASDSGIGKACALLLAQNGFDIGITWHSDERGAQETAKKAAQFGVRAETIHLDLS 62
K+A +T + GIG+A A LA G I ++ E+ + + A+ AE D+
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE-ARHAEAFPADVR 67

Query: 63 QLPEGAQAIEHLIQRLGRVDVLVNNAGAMTKSAFIDMPFTQWRQIFTVDVDGAFLCAQIA 122
+ + + +G +D+LVN AG + + +W F+V+ G F ++
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 123 ARHMIKQGEGGRIINITSVHEHTPLPQASAYTAAKHALGGLTKSMALELIEYHILVNAVA 182
+++M+ + G I+ + S P +AY ++K A TK + LEL EY+I N V+
Sbjct: 128 SKYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 183 PGAIATPM-------NDMDDSDIEPGSEP---SIPIARPGSTHEIASLVAWLCSEGASYT 232
PG+ T M + + I+ E IP+ + +IA V +L S A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 233 TDQSLIVDGGFML 245
T +L VDGG L
Sbjct: 247 TMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0681BCTERIALGSPF270.034 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 27.5 bits (61), Expect = 0.034
Identities = 8/39 (20%), Positives = 16/39 (41%), Gaps = 1/39 (2%)

Query: 161 WLHDLDQHLRH-GVWLILAIVLVVGVRWWLKRRGKAEAR 198
L + +R G W++LA++ + R+ K
Sbjct: 215 VLMGMSDAVRTFGPWMLLALLAGFMAFRVMLRQEKRRVS 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0683BLACTAMASEA375e-05 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 37.1 bits (86), Expect = 5e-05
Identities = 38/180 (21%), Positives = 67/180 (37%), Gaps = 6/180 (3%)

Query: 11 FALMLAVPFAPQAVAKTAATTAASQPEIASGSAMI-VDLNTNKVIYSNHPDLVRPIASIT 69
+L+ +P A A + S+ +++ MI +DL + + + + D P+ S
Sbjct: 9 ISLLATLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTF 68

Query: 70 KLMTAMVVLDARLPLDEILKVDISQTPEMKGVYSRV---RLNSEISRKNMLLLALMSSEN 126
K++ VL DE L+ I + YS V L ++ + A+ S+N
Sbjct: 69 KVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCAAAITMSDN 128

Query: 127 RAAASLAHYY--PGGYNAFIKAMNAKAKALGMTHTRFVEPTGLSIHNVSTARDLTKLLIA 184
AA L P G AF++ + L T E + +T + L
Sbjct: 129 SAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTTPASMAATLRK 188


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0692PF065802191e-68 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 219 bits (559), Expect = 1e-68
Identities = 60/216 (27%), Positives = 116/216 (53%), Gaps = 3/216 (1%)

Query: 343 LGEGIAQLLSAQILAGQYERQKALLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQA 402
L G + + + ++ ++++ L AQ+NPHF+FNALN I+A+I D +A
Sbjct: 134 LYFGWHFFKNYKQAEIDQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKA 193

Query: 403 SQLVQYLSTFFRKNLKR-PSEIVTLADEIEHVNAYLQIEKARFQSRLQVQLDVPSTLSRQ 461
+++ LS R +L+ + V+LADE+ V++YLQ+ +F+ RLQ + + +
Sbjct: 194 REMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDV 253

Query: 462 KLPAFTLQPIVENAIKHGTSQLLDTGNVAIRARREGQHLMLDIEDNAGLYQPSAG-SSGL 520
++P +Q +VEN IKHG +QL G + ++ ++ + L++E+ L + S+G
Sbjct: 254 QVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGT 313

Query: 521 GMSLVDKRLREHFGDDYGISVACEPDCFTRITLRLP 556
G+ V +RL+ +G + I ++ + + +P
Sbjct: 314 GLQNVRERLQMLYGTEAQIKLSEKQGKVN-AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0693HTHFIS766e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.6 bits (186), Expect = 6e-18
Identities = 49/215 (22%), Positives = 87/215 (40%), Gaps = 19/215 (8%)

Query: 2 IKVLIVDDEPLARENLRILLQGQDDIEIVGECANAVEAIGAVHKLRPDVLFLDIQMPRIS 61
+L+ DD+ R L L V +NA + D++ D+ MP +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 62 GLEMVGMLDPEHRPYI--VFLTAFD--EYAIKAFEEHAFDYLLKPIEEKRLEKTLHRLRQ 117
+++ + + RP + + ++A + AIKA E+ A+DYL KP + L + R
Sbjct: 62 AFDLLPRIK-KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 118 ERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMDDVAFVSSRMSGVYVT--SSEGKEGFT 175
E ++ L ++Q + + G S +A + + +T S GK
Sbjct: 121 EPKRRPSKLEDDSQDGMPLV---GRSAAMQEIYRVLARLMQTDLTLMITGESGTGK---- 173

Query: 176 ELTLRTLESRTPLLRCHRQFL-VNMAHLQEIRLED 209
EL R L R + F+ +NMA + +E
Sbjct: 174 ELVARALHDYGK--RRNGPFVAINMAAIPRDLIES 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0700FIMBRIALPAPE280.012 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 28.5 bits (63), Expect = 0.012
Identities = 36/134 (26%), Positives = 59/134 (44%), Gaps = 16/134 (11%)

Query: 3 RSLIVASVLSAVFMSAGVFAADEDMGELKINGEVVGTSCTFEGANSATIELSQVGVDRLT 62
R L + +L AV MS V AAD L G+++ +CT + +A + + + L
Sbjct: 5 RGLCLPVMLGAVLMSQHVHAAD----NLTFKGKLIIPACTVQ---NAEVNWGDIEIQNLV 57

Query: 63 DL--NPGDIYTGYTSPEAILKVKCSNTANPR------ISFNRSQFVDNMQITKNNATNNG 114
N D P ++ +K + T+N + + + D + I N+ N+G
Sbjct: 58 QSGGNQKDFTVDMNCPYSLGTMKVTITSNGQTGNSILVPNTSTASGDGLLIYLYNSNNSG 117

Query: 115 AGFAVYLDGTQVKP 128
G AV L G+QV P
Sbjct: 118 IGNAVTL-GSQVTP 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0702PF005776750.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 675 bits (1742), Expect = 0.0
Identities = 239/829 (28%), Positives = 389/829 (46%), Gaps = 28/829 (3%)

Query: 13 AALLISPAWAEEETFDTNFMFG-GLKGEKVSRYQIDSTKPMAGVYEMDVYVNKEWRGTYE 71
A +P + E F+ F+ +SR++ + + G Y +D+Y+N + T +
Sbjct: 35 AFAAQAPLSSAELYFNPRFLADDPQAVADLSRFE-NGQELPPGTYRVDIYLNNGYMATRD 93

Query: 72 VNIQDDPDST----CISPDLIASLGIK---FTPQSTTVENECIALKTVVHGGSVSYDTAA 124
V C++ +AS+G+ + + ++ C+ L +++H + D
Sbjct: 94 VTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQ 153

Query: 125 FNLYLSVPQAYVLEYEAGYASPETWDRGINAFYTSYYASEYYSHYKSGGSEKNTYANFVS 184
L L++PQA++ GY PE WD GINA +Y S + GG+ Y N S
Sbjct: 154 QRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQS 213

Query: 185 GLNLLGWQLHSNANFSKSEN-----LAGKWQSNTQYLERDFPAVLGTMRLGEQYTSGDMF 239
GLN+ W+L N +S + + KWQ +LERD + + LG+ YT GD+F
Sbjct: 214 GLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIF 273

Query: 240 DTVRFRGVRFWRDMQMLPHSKQNFAPVVRDVAQSNALVTVEQNGFIVYQKEVPPGPFVFE 299
D + FRG + D MLP S++ FAPV+ +A+ A VT++QNG+ +Y VPPGPF
Sbjct: 274 DGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIN 333

Query: 300 DLQLAGGGADLDVSVKEADGTVSRFIVPYSSVPNMVQPGVAKYDFAAGRSRIEGASQQTD 359
D+ AG DL V++KEADG+ F VPYSSVP + + G +Y AG R A Q+
Sbjct: 334 DIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKP 393

Query: 360 -FLQGTYQYGVNNLLTLYGGTMLASDYRSFTLGTGWNT-LIGAVSVDGTLSHSKQDNGDV 417
F Q T +G+ T+YGGT LA YR+F G G N +GA+SVD T ++S +
Sbjct: 394 RFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPDDSQ 453

Query: 418 FDGESYQVAWNKYLPQSATHFSLAAYRYSSRDYRTFNDHVWANNRDNYRRDDDDIYDI-- 475
DG+S + +NK L +S T+ L YRYS+ Y F D ++ D + +
Sbjct: 454 HDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKP 513

Query: 476 --ADYYENDFGRKNTFTLNINQTLPDGWGYFTASALWRDYWGRSGTGKDYQLSYSNTWQR 533
DYY + ++ L + Q L S + YWG S + +Q + ++
Sbjct: 514 KFTDYYNLAYNKRGKLQLTVTQQLGR-TSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFED 572

Query: 534 LSFTLSATQTYDSDNRE-DKRFNIYLSIPL--TWGVKENGGNRDIHLSNSTTFDDQGYEA 590
+++TLS + T ++ + D+ + ++IP R S S + D G
Sbjct: 573 INWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMT 632

Query: 591 NNTSLSGTFGNRDQFNYTTNLS---QQRQEHQTTFGGSVTWNAPLATVGGSYSQSNKYHQ 647
N + GT + +Y+ +T ++ + YS S+ Q
Sbjct: 633 NLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQ 692

Query: 648 VGGNIQGGLVAWADGVHLASRLNDTIAIINAPYLEGAAVQGRPYLRTNAKGYAVFEALTP 707
+ + GG++A A+GV L LNDT+ ++ AP + A V+ + +RT+ +GYAV T
Sbjct: 693 LYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATE 752

Query: 708 YRQNFISLDVSGSESDVALLGNRKVTVPYRGAVVVVDFETETSKPFYFLARRADGEPLTF 767
YR+N ++LD + +V L VP RGA+V +F+ + +PL F
Sbjct: 753 YRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTH-NNKPLPF 811

Query: 768 GYEVEDDEGNNVGLVGQGSRVFIRTEKVPISVKVATDKQQGLFCKITFD 816
G V + + G+V +V++ + V+V +++ C +
Sbjct: 812 GAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQ 860


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0704TYPE3OMGPROT270.019 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 26.8 bits (59), Expect = 0.019
Identities = 10/43 (23%), Positives = 17/43 (39%), Gaps = 7/43 (16%)

Query: 3 SKLLPCALLLATSFAWAAPA-------TTGIDQYELKSFIADF 38
++L LLL +S++WA L+ + DF
Sbjct: 10 KRVLTGTLLLLSSYSWAQELDWLPIPYVYVAKGESLRDLLTDF 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0710TCRTETA362e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 36.3 bits (84), Expect = 2e-04
Identities = 33/153 (21%), Positives = 52/153 (33%), Gaps = 20/153 (13%)

Query: 253 FSEIFFMLALPFFTKRFGIKKVLLLGLVTAAIRYGFFVYGGAETYFTYALLFLGILLHGV 312
+ L + RFG + VLL+ L AA+ Y +L++G ++ G+
Sbjct: 54 LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAP-----FLWVLYIGRIVAGI 108

Query: 313 SYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPQPVN 372
+ V D R G ++ C GFG + G LGG+M P
Sbjct: 109 TGATGAVAGAYIAD-ITDGDERARHFGFMS-ACFGFGMVAGPVLGGLMGGFSPHAP---- 162

Query: 373 GLTFNWAGMWTFGAVMIAVIALLFMIFFRESDK 405
+ A + + L ES K
Sbjct: 163 ---------FFAAAALNGLNFLTGCFLLPESHK 186



Score = 32.9 bits (75), Expect = 0.002
Identities = 54/286 (18%), Positives = 93/286 (32%), Gaps = 17/286 (5%)

Query: 29 LNKSGFSAGEIGWSYACTAIAAILSPILVGSVTDRFFSAQKVLAVLMFAGAVLMYFAAQQ 88
L S G A A+ ++G+++DRF ++ + ++ AGA + Y
Sbjct: 35 LVHSNDVTAHYGILLALYALMQFACAPVLGALSDRF--GRRPVLLVSLAGAAVDYAI--- 89

Query: 89 TTFAGFFPLLLAYSLTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIG-WIASGLACGF 147
A F +L + T A T ++A A + D+ R R G + G+ G
Sbjct: 90 MATAPFLWVLYIGRIVAGITGA-TGAVAGAYIADITDGDERARHFGFMSACFGFGMVAG- 147

Query: 148 LPQMLGY-NDISPTNIPLLITAASSALLGVFAFCLPDTPPKSTGKMDIKVMLGLDALILL 206
P + G SP + P AA + L + L K + + L A
Sbjct: 148 -PVLGGLMGGFSP-HAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRW 205

Query: 207 RDKN------FLVFFFCSFLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFML 260
VFF + +P A + IF G + +
Sbjct: 206 ARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAM 265

Query: 261 ALPFFTKRFGIKKVLLLGLVTAAIRYGFFVYGGAETYFTYALLFLG 306
R G ++ L+LG++ Y + ++ L
Sbjct: 266 ITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLA 311


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0713RTXTOXINA270.020 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 26.9 bits (59), Expect = 0.020
Identities = 19/75 (25%), Positives = 24/75 (32%), Gaps = 2/75 (2%)

Query: 32 FNAYGNKPRCLMCLGTTALFTGVFSGVCSGAVASVSSGAAYTTALTVLGASFGLGG--IG 89
N GNK + L L SG+ S AS A T A L +G
Sbjct: 222 LNGVGNKLQNLPNLDNIGAGLDTVSGILSAISASFILSNADADTRTKAAAGVELTTKVLG 281

Query: 90 MMGICAGLYLSANGV 104
+G Y+ A
Sbjct: 282 NVGKGISQYIIAQRA 296


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0714PF05932845e-24 Tir chaperone protein (CesT)
		>PF05932#Tir chaperone protein (CesT)

Length = 127

Score = 83.7 bits (207), Expect = 5e-24
Identities = 29/118 (24%), Positives = 45/118 (38%), Gaps = 3/118 (2%)

Query: 6 DRLLRQFSLKLNADSIAFDENRLCSFIIDNRYRI-LLTSTNSEYIMIYGFCGRPPDNNNL 64
LL FS L + FD++ C+ IIDN + + L E +++ G P +
Sbjct: 7 KTLLDDFSRSLEMQPLVFDDHGTCNMIIDNTFALTLSCDYARERLLLIGLLE--PHKDIP 64

Query: 65 AFEFLNSNLWFAENNGPHLCYDNNSQSLLLALNFSLNESSVEKLECEIEVVIRSMENL 122
L L N GP L D S + + SV L+ E+ ++ M
Sbjct: 65 QQCLLAGALNPLLNAGPGLGLDEKSGLYHAYQSIPREKLSVPTLKREMAGLLEWMRGW 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0734HTHFIS758e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.3 bits (185), Expect = 8e-18
Identities = 27/140 (19%), Positives = 65/140 (46%), Gaps = 2/140 (1%)

Query: 11 PRILIVEDEPKLGQLLIDYLRAASYAPTLINHGDKVLPYVRQTPPDLILLDLMLPGTDGL 70
IL+ +D+ + +L L A Y + ++ + ++ DL++ D+++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 71 TLCREIR-RFSDIPIVMVTAKIEEIDRLLGLEIGADDYICKPYSPREVVARVKTIL-RRC 128
L I+ D+P+++++A+ + + E GA DY+ KP+ E++ + L
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 129 KPQRELQQQDAESPLMIDES 148
+ +L+ + ++ S
Sbjct: 124 RRPSKLEDDSQDGMPLVGRS 143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0735BCTERIALGSPF310.010 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 31.0 bits (70), Expect = 0.010
Identities = 20/66 (30%), Positives = 26/66 (39%), Gaps = 14/66 (21%)

Query: 187 RGLLAPVKRLVEGTHRLAAGDFTTRVTPTSADEL-----------GKLAQDFNQLASTLE 235
L+A V+ V H LA + P S + L G L N+LA E
Sbjct: 104 SQLMAAVRSKVMEGHSLAD---AMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTE 160

Query: 236 KNQQMR 241
+ QQMR
Sbjct: 161 QRQQMR 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0736TCRTETB1243e-33 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 124 bits (313), Expect = 3e-33
Identities = 95/450 (21%), Positives = 197/450 (43%), Gaps = 25/450 (5%)

Query: 20 FMQSLDTTIVNTALPSMAKSLGESPLHMHMVVVSYVLTVAVMLPASGWLADKIGVRNIFF 79
F L+ ++N +LP +A + P + V +++LT ++ G L+D++G++ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 80 AAIVLFTLGSLFCALSGTLNQ-LVLARVLQGVGGAMMVPVGRLTVMKIVPRAQYMAAMTF 138
I++ GS+ + + L++AR +QG G A + + V + +P+ A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 139 VTLPGQIGPLLGPALGGVLVEYASWHWIFLINIP-VGIVGAMATFMLMPNYTIETRRFDL 197
+ +G +GPA+GG++ Y HW +L+ IP + I+ L+ FD+
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 198 PGFLLLAIGMAVLTLALDGSKSMGISPWTLAGLAAGGAAAILLYLFHAKKSSGALFSLRL 257
G +L+++G+ L + L + L+++ H +K + L
Sbjct: 202 KGIILMSVGIVFFMLFTTSYSISFLIVSVL---------SFLIFVKHIRKVTDPFVDPGL 252

Query: 258 FRTPTFSLGLLGSFAGRIGSGMLPFMTPVFLQIGLGFSPFHAG-LMMIPMVLGSMGMKRI 316
+ F +G+L M P ++ S G +++ P + + I
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312

Query: 317 VVQIVNRFGYRRVLVATTLGLALVSLLFMSVALL----GWYYLLPLVLLLQGMVNSARFS 372
+V+R G VL +G+ +S+ F++ + L W+ + +V +L G+ S +
Sbjct: 313 GGILVDRRGPLYVL---NIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL--SFTKT 367

Query: 373 SMNTLTLKDLPDTLASSGNSLLSMIMQLSMSIGVTIAGMLL--GMFGQQHIGIDSSATHH 430
++T+ L A +G SLL+ LS G+ I G LL + Q+ + ++ + +
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTY 427

Query: 431 VFMYTWLCMAVIIALPAIIFARVPNDTQQN 460
++ L + II + ++ V +Q++
Sbjct: 428 LYSNLLLLFSGIIVISWLVTLNVYKHSQRD 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0737ACRIFLAVINRP8750.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 875 bits (2263), Expect = 0.0
Identities = 282/1035 (27%), Positives = 503/1035 (48%), Gaps = 36/1035 (3%)

Query: 6 LFIYRPVATILIAAAITLCGILGFRLLPVAPLPQVDFPVIMVSASLPGASPETMASSVAT 65
FI RP+ ++A + + G L LPVA P + P + VSA+ PGA +T+ +V
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 66 PLERSLGRIAGVNEMTSSS-SLGSTRIILEFNFDRDINGAARDVQAAINAAQSLLPGGMP 124
+E+++ I + M+S+S S GS I L F D + A VQ + A LLP +
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 125 SRPTYRKANPSDAPIMILTLTSES--WSQGKLYDFASTQLAQTIAQIDGVGDVDVGGSSL 182
+ S + +M+ S++ +Q + D+ ++ + T+++++GVGDV + G+
Sbjct: 124 -QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY 182

Query: 183 PAVRVGLNPQALFNQGVSLDEVREAIDSANVRRPQGAIEDSV------HRWQIQTNDELK 236
A+R+ L+ L ++ +V + N + G + + I K
Sbjct: 183 -AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 237 TAAEYQPLIIHYN-NGAAVRLGDVASVTDSVQDVRNAGMTNAKPAILLMIRKLPEANIIQ 295
E+ + + N +G+ VRL DVA V ++ N KPA L I+ AN +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 296 TVDGIRAKLPELRAMIPAAIDLQIAQDRSPTIRASLQEVEETLAISVALVILVVFLFLRS 355
T I+AKL EL+ P + + D +P ++ S+ EV +TL ++ LV LV++LFL++
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 356 GRATLIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMALTIATGFVVDDAIVVLENIARHL- 414
RATLIP +AVPV L+GTFA + G+S+N L++ + +A G +VDDAIVV+EN+ R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 EAGMKPLQAALQGTREVGFTVISMSLSLVAVFLPLLLMGGLPGRLLREFAVTLSVAIGIS 474
E + P +A + ++ ++ +++ L AVF+P+ GG G + R+F++T+ A+ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 475 LVVSLTLTPMMCGWMLKSSKPRTQPRKRGVG----RLLVALQQGYGTSLKWVLNHTRLVG 530
++V+L LTP +C +LK K G Y S+ +L T
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 531 VVFLGTVALNIWLYIAIPKTFFPEQDTGVLMGGIQADQSISFQ----AMRGKLQDFMKII 586
+++ VA + L++ +P +F PE+D GV + IQ + + + ++K
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 587 RD-DPAVNNVTGFT-GGSRVNSGMMFITLKPRGER---KETAQQIIDRLRVKLAKEPGAR 641
+ +V V GF+ G N+GM F++LKP ER + +A+ +I R +++L K
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 642 LFLMAVQDIRVGGRQANASYQYTLLSDSLPALREWEPKIRKALSAL-----PQLADVNSD 696
+ + I G ++ L D + + R L + L V +
Sbjct: 662 VIPFNMPAIVELGTATGFDFE---LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 697 QQDNGAEMNLIYDRDTMSRLGIDVQAANSLLNNTFGQRQISTIYQPMNQYKVVMEVDPRY 756
++ A+ L D++ LG+ + N ++ G ++ K+ ++ D ++
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 757 TQDISALEKMFVINRDGKAIPLSYFAQWRPANAPLSVNHQGLSAASTIAFNLPTGTSLSQ 816
++K++V + +G+ +P S F + + I GTS
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 817 ATEAINRTMTQLGVPSTVRGSFSGTAQVFQQTMNSQLILIVAAIATVYIVLEILYESYVH 876
A + ++L P+ + ++G + + + N L+ + V++ L LYES+
Sbjct: 839 AMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSI 896

Query: 877 PLTILSTLPSASVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRSGG 936
P++++ +P VG LLA LFN + ++G++ IG+ KNAI++V+FA + G
Sbjct: 897 PVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 937 LTPEQAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLGITIVGGLVMSQLL 996
+A A +R RPI+MT+LA + G LPL +S G GS + +GI ++GG+V + LL
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 997 TLYTTPVVYLFFDRL 1011
++ PV ++ R
Sbjct: 1017 AIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0738ACRIFLAVINRP8850.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 885 bits (2289), Expect = 0.0
Identities = 292/1036 (28%), Positives = 504/1036 (48%), Gaps = 29/1036 (2%)

Query: 13 SRLFILRPVATTLLMAAILLAGIIGYRFLPVAALPEVDYPTIQVVTLYPGASPDVMTSSV 72
+ FI RP+ +L +++AG + LPVA P + P + V YPGA + +V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 73 TAPLERQFGQMSGLKQMSSQS-SGGASVVTLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131
T +E+ + L MSS S S G+ +TL FQ D+A+ +VQ + AT LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 132 LPNPPIYSKVNPADPPIMTLAVTSNAMPMTQVE--DMVETRVAQKISQVSGVGLVTLAGG 189
+ I S + +M S+ TQ + D V + V +S+++GVG V L G
Sbjct: 122 VQQQGI-SVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 190 QRPAIRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSLDGP------ERAVTLSANDQ 243
Q A+R+ L+A + LT V + N A G L G + ++ A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 244 MQSADEYRKLII-AYQNGAPVRLGDVATVEQGAENSWLGAWANQAPAIVMNVQRQPGANI 302
++ +E+ K+ + +G+ VRL DVA VE G EN + A N PA + ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 303 IATADSIRQMLPQLTESLPKSVKVTVLSDRTTNIRASVRDTQFELMLAIALVVMIIYLFL 362
+ TA +I+ L +L P+ +KV D T ++ S+ + L AI LV +++YLFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 363 RNIPATIIPGVAVPLSLIGTFAVMVFLDFSINNLTLMALTIATGFVVDDAIVVIENISRY 422
+N+ AT+IP +AVP+ L+GTFA++ +SIN LT+ + +A G +VDDAIVV+EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 423 I-EKGEKPLAAALKGAGEIGFTIISLTFSLIAVLIPLLFMGDIVGRLFREFAVTLAVAIL 481
+ E P A K +I ++ + L AV IP+ F G G ++R+F++T+ A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 482 ISAVVSLTLTPMMCACML---SQQSLRKQNRFSRACERMFDRVIASYGRGLAKVLNHPWL 538
+S +V+L LTP +CA +L S + + F FD + Y + K+L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 539 TLSVAFATLLLSVMLWIVIPKGFFPVQDNGIIQGTLQAPQSSSYASMAQRQRQVAERILQ 598
L + + V+L++ +P F P +D G+ +Q P ++ + QV + L+
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 599 DPA--VQSLTTFVGVDGANPTLNSARLQINLKPLDARDDR---VQQVISRLQTAVATIPV 653
+ V+S+ T G + N+ ++LKP + R+ + VI R + + I
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 654 VALYLQPTQDLTIDTQVSRTQYQFTLQ---ATTLDALSHWVPKL-QNALQSLPQLSEVSS 709
++ P I + T + F L DAL+ +L A Q L V
Sbjct: 660 G--FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRP 717

Query: 710 DWQDRGLAAWVNVDRDSASRLGISIADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTA 769
+ + + VD++ A LG+S++D++ + A G ++ + ++ ++ +
Sbjct: 718 NGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAK 777

Query: 770 STPGLAALETIRLTSRDGGTVSLSAIARIEQRFAPLSINHLDQFPVTTFSFNVPEGYSLG 829
++ + + S +G V SA + + + P G S G
Sbjct: 778 FRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG 837

Query: 830 DAVQAILDTEKTLALPADITTQFQGSTLAFQAALGSTVWLIVAAVVAMYIVLGVLYESFI 889
DA+ + + LPA I + G + + + L+ + V +++ L LYES+
Sbjct: 838 DAMALMENLAS--KLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWS 895

Query: 890 HPITILSTLPTAGVGALLALIIAGSELDIIAIIGIILLIGIVKKNAIMMIDFALAAEREQ 949
P++++ +P VG LLA + + D+ ++G++ IG+ KNAI++++FA ++
Sbjct: 896 IPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKE 955

Query: 950 GMSPRDAIFQACLLRFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIAMVGGLLVSQV 1009
G +A A +R RPILMT+LA +LG LPL +S G G+ + +GI ++GG++ + +
Sbjct: 956 GKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATL 1015

Query: 1010 LTLFTTPVIYLLFDRL 1025
L +F PV +++ R
Sbjct: 1016 LAIFFVPVFFVVIRRC 1031


15SPA0751SPA0789Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0751215-2.898682hypothetical protein
SPA0752115-1.660260acetyltransferase
SPA0753016-0.760715glycosyltransferase
SPA0754016-0.852130colanic acid polymerase
SPA07550182.474797glycosyltransferase
SPA0756-1244.469483acetyltransferase
SPA07570325.955715GDP-mannose 4,6-dehydratase
SPA0758-1284.780411GDP-fucose synthetase
SPA0759-1264.357348O-antigen biosynthesis protein
SPA07600213.020116glycosyltransferase
SPA07610171.212820mannose-1-phosphate guanylyltransferase
SPA0762-113-0.422088phosphomannomutase
SPA0764-116-3.304395transmembrane transport protein
SPA0766-120-4.605747glycosyl transferase family protein
SPA0767129-7.431933hypothetical protein
SPA0769437-9.111934dTDP-glucose 4,6-dehydratase
SPA0770540-9.196286dTDP-4-dehydrorhamnose reductase
SPA0771842-10.181874TDP-glucose pyrophosphorylase
SPA0772946-11.903907dTDP-4-dehydrorhamnose 3,5-epimerase
SPA0773949-12.863714reductase RfbI
SPA0774752-14.712838glucose-1-phosphate cytidylyltransferase
SPA0775755-16.252676CDP-glucose 4,6-dehydratase
SPA0776859-18.248413dehydratase RfbH
SPA0777864-20.507307paratose synthase
SPA0778865-20.719682CDP-tyvelose-2-epimerase
SPA0779665-21.098343O-antigen transporter
SPA0780663-20.717344glycosyl transferase family protein
SPA0781662-20.044223glycosyltransferase
SPA0782458-18.268518glycosyl transferase family protein
SPA0783457-16.802879glycosyltransferase
SPA0784456-15.927411glycosyl transferase family protein
SPA0785244-12.657712glycosyltransferase
SPA0786240-11.401833rhamnosyltransferase
SPA0787134-9.694400mannose-1-phosphate guanylyltransferase
SPA0788128-8.220911phosphomannomutase
SPA0789-219-6.014695undecaprenyl-phosphate
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0757NUCEPIMERASE1062e-28 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 106 bits (267), Expect = 2e-28
Identities = 78/361 (21%), Positives = 120/361 (33%), Gaps = 58/361 (16%)

Query: 6 LITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPH--------SCNPK 57
L+TG G G ++++ LLE G++V GI + N Y D P
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGI----DNLND------YYDVSLKQARLELLAQPG 53

Query: 58 FHLHYGDLTDASNLTRILQEVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAI 117
F H DL D +T + + V+ V S E+P AD + G L +LE
Sbjct: 54 FQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGC 113

Query: 118 RFLGLEKKTRFYQASTSELYGLVQEIPQKETTPF-YPRSPYAVAKLYAYWITVNYRESYG 176
R ++ AS+S +YGL +++P +P S YA K + Y YG
Sbjct: 114 RHNKIQ---HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170

Query: 177 IYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVRM 236
+ A F P K T+A+ G +Y RD+ + D
Sbjct: 171 LPATGLRFFTVYGPWGRPDMALFKFTKAMLE---GKSIDVY-NYGKMKRDFTYIDDIAEA 226

Query: 237 QWMMLQQEQPEDFVIATGVQYSVRQFVELAAVQLGIKLRFEGEGINEKGIVVSVTGHDAP 296
+ D N G+ +P
Sbjct: 227 IIRLQDVIPHADTQWTVETGTPAAS-------------IAPYRVYN--------IGNSSP 265

Query: 297 GVKPGDVIVAV--------DPRY--FRPAEVETLLGDPSKAHEKLGWKPEITLSEMVSEM 346
V+ D I A+ +P +V D +E +G+ PE T+ + V
Sbjct: 266 -VELMDYIQALEDALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNF 324

Query: 347 V 347
V
Sbjct: 325 V 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0758NUCEPIMERASE886e-22 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 87.9 bits (218), Expect = 6e-22
Identities = 64/344 (18%), Positives = 128/344 (37%), Gaps = 47/344 (13%)

Query: 5 RIFVAGHRGMVGSAIVRQLAQRG-------------DVEL------VLRTRD----ELDL 41
+ V G G +G + ++L + G DV L +L ++DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 42 LDGRAVQAFFARAGIDQVYLAAAKVGGIVANNTYPADFIYENMMIESNIIHAAHLHNVNK 101
D + FA ++V+++ + + + P + N+ NI+ + +
Sbjct: 62 ADREGMTDLFASGHFERVFISPHR-LAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 102 LLFLGSSCIYPKLARQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSV 161
LL+ SS +Y + P + P + YA K A + +Y+ YG +
Sbjct: 121 LLYASSSSVYGLNRKMPFSTD---DSVDHPVS-LYAATKKANELMAHTYSHLYGLPATGL 176

Query: 162 MPTNLYGPHDNFHPDNSHVIPALLRRFHEAAQSHAPEVVVWGSGTPMREFLHVDDMAAAS 221
+YGP PD AL + + + +V + G R+F ++DD+A A
Sbjct: 177 RFFTVYGPWGR--PDM-----ALFKFTKAMLEGKSIDV--YNYGKMKRDFTYIDDIAEAI 227

Query: 222 IHVMELA----REVWQENTAPMLSH-----INVGTGVDCTIRELAQTIAKVVGYQGRVVF 272
I + ++ + E P S N+G + + Q + +G + +
Sbjct: 228 IRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNM 287

Query: 273 DAAKPDGTPRKLLDVTRLHQ-LGWYHEISLEAGLAGTYQWFLEN 315
+P D L++ +G+ E +++ G+ W+ +
Sbjct: 288 LPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0769NUCEPIMERASE1761e-54 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 176 bits (449), Expect = 1e-54
Identities = 83/359 (23%), Positives = 142/359 (39%), Gaps = 50/359 (13%)

Query: 1 MKILITGGAGFIGSAVVRHIIKNTQDTVVNIDKLT--YAGNLE--SLSDISESNRYNFEH 56
MK L+TG AGFIG V + +++ VV ID L Y +L+ L +++ + F
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAG-HQVVGIDNLNDYYDVSLKQARLELLAQPG-FQFHK 58

Query: 57 ADICDSAEITRIFEQYQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEVARKYWS 116
D+ D +T +F + V V S+ P A+ ++N+ G +LE R
Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN-- 116

Query: 117 ALGEDKKNNFRFHHISTDEVYGDLPHPDEVENSVTLPLFTETTAYAPSSPYSASKASSDH 176
+ S+ VYG +P T+ + P S Y+A+K +++
Sbjct: 117 -------KIQHLLYASSSSVYGLNRK---------MPFSTDDSVDHPVSLYAATKKANEL 160

Query: 177 LVRAWRRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYV 236
+ + YGLP YGP+ P+ + LEGK + +Y G RD+ Y+
Sbjct: 161 MAHTYSHLYGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYI 220

Query: 237 EDHA----RALHMVVTEGKA--------------GETYNIGGHNEKKNLDVVFTICDLLD 278
+D A R ++ YNIG + + +D + + D L
Sbjct: 221 DDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALG 280

Query: 279 EIVPKATSYREQITYVADRPGHDRRYAIDAGKISRELGWKPLETFESGIRKTVEWYLAN 337
+A + + +PG + D + +G+ P T + G++ V WY
Sbjct: 281 ---IEA-----KKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0770NUCEPIMERASE413e-06 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 40.9 bits (96), Expect = 3e-06
Identities = 27/160 (16%), Positives = 57/160 (35%), Gaps = 23/160 (14%)

Query: 1 MNILLFGKTGQVGWELQRSLAPVGN-LIALDV-----------HSKEFC---------GD 39
M L+ G G +G+ + + L G+ ++ +D E D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 40 FSNPKGVAETVRKLRPDVIVNAAAHTAVDKAESEPELAQLLNATSVEAIAKAANEIG-AW 98
++ +G+ + + + + AV + P N T I +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 99 VVHYSTDYVFPGTGDIPWQETDATS-PLNVYGKTKLAGEK 137
+++ S+ V+ +P+ D+ P+++Y TK A E
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0775NUCEPIMERASE731e-16 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 73.3 bits (180), Expect = 1e-16
Identities = 62/352 (17%), Positives = 121/352 (34%), Gaps = 48/352 (13%)

Query: 11 RVFVTGHTGFKGSWLSLWLTEMGAIVKGYALDAPTVPSLFEIVRLNDLMES----HIGDI 66
+ VTG GF G +S L E G V G + RL L + H D+
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 67 RDFEKLRSSIAEFKPEIVFHMAAQPLVRLSYEQPIETYSTNVMGTVHLLEAVKQVDNIKA 126
D E + A E VF + VR S E P +N+ G +++LE + I+
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN-KIQH 120

Query: 127 VVNITSDKCYDNREWVWGYRENEPMGGYD-------PYSNSKGCAELVASAFRNSFFNPA 179
++ +S V+G P D Y+ +K EL+A + + +
Sbjct: 121 LLYASSSS-------VYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLY---- 169

Query: 180 NYEQHGVGLASVRAGNVIGGGDWAK-DRLIPDILRSFENNQQVIIRNPYSI-RPWQHVLE 237
G+ +R V G W + D + ++ + + + N + R + ++ +
Sbjct: 170 -----GLPATGLRFFTVY--GPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDD 222

Query: 238 PLSGYIVVAQRLYTEGAKFSEG-------------WNFGPRDEDAKTVEFIVDKMVTLWG 284
I + + +++ +N G + + + + G
Sbjct: 223 IAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIG--NSSPVELMDYIQALEDALG 280

Query: 285 DDASWLLDGENHPHEAHYLKLDCSKANMQLGWHPRWGLTETLSRIVKWHKAW 336
+A + P + D +G+ P + + + V W++ +
Sbjct: 281 IEAKKNML-PLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0776PERTACTIN310.012 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 30.8 bits (69), Expect = 0.012
Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 9/82 (10%)

Query: 209 GDIGTVSFYPAHHITMGEGGAVFTKSGELKKIIESFRDWGRDCYCAPGCDNTCGKRFGQQ 268
G +G S + E A+ + GEL+ ++ WGR DN G+RF Q+
Sbjct: 629 GGVGLAS-----TLWYAESNALSKRLGELRLNPDAGGAWGRGFAQRQQLDNRAGRRFDQK 683

Query: 269 LGSLPQGYDHKYTYS----HLG 286
+ G DH + HLG
Sbjct: 684 VAGFELGADHAVAVAGGRWHLG 705


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0777NUCEPIMERASE646e-14 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 64.0 bits (156), Expect = 6e-14
Identities = 58/329 (17%), Positives = 115/329 (34%), Gaps = 57/329 (17%)

Query: 1 MKILIMGAFGFLGSRLTSYFESR-HTVIGL---------ARKRNNEATINNIIYT----- 45
MK L+ GA GF+G ++ H V+G+ + K+ + +
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 46 -TENNWIEKIL-EFEPNIIINTIACYG-RHN-EPATALIESNILMPIRVLE--------- 92
+ + + + + R++ E A +SN+ + +LE
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 93 ----SISSL--DAVFINCGTSLPPNT--SLYAYTKQKANEFAAAIIDKVCG-KYIELKLE 143
S SS+ + T + SLYA TK KANE A + G L+
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATK-KANELMAHTYSHLYGLPATGLRFF 179

Query: 144 HFYGAFDGDDKFTSMVIRRCLSNQPVKL-TSGLQQRDFLYIKDL----LTAFDCIISNVN 198
YG + D + L + + + G +RDF YI D+ + D I
Sbjct: 180 TVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADT 239

Query: 199 NFPKFHS-----------IEVGSGEATSIREYVETVKNITKSNSIIEFGVVKERVNELMY 247
+ +G+ + +Y++ +++ + + + +++
Sbjct: 240 QWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNM--LPLQPGDVLE 297

Query: 248 SCADIAELEK-IGWKREFSLVDALTEIIE 275
+ AD L + IG+ E ++ D + +
Sbjct: 298 TSADTKALYEVIGFTPETTVKDGVKNFVN 326


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0778NUCEPIMERASE1572e-47 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 157 bits (398), Expect = 2e-47
Identities = 84/355 (23%), Positives = 151/355 (42%), Gaps = 55/355 (15%)

Query: 9 LITGGCGFLGSNLASFALSQGIDLIVFDNL------SRKGATDNLHWLSSLGNFEFVHGD 62
L+TG GF+G +++ L G ++ DNL S K A L L+ F+F D
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQA--RLELLAQ-PGFQFHKID 60

Query: 63 IRNKNDVTRLITKYMPDSCFHLAGQVAMTTSIDNPCMDFEINVGGTLNLLEAVRQYNSNC 122
+ ++ +T L + F ++A+ S++NP + N+ G LN+LE R
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ- 119

Query: 123 NIIYSSTNKVYGDLEQYKYNETETRYTCVDKPNGYDESTQLDFHSPYGCSKGAADQYMLD 182
+++Y+S++ VYG + ++ ++ VD P S Y +K A +
Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDS----VDHP-----------VSLYAATKKANELMAHT 164

Query: 183 YARIFGLNTVVFRHSSMYG--GRQFATYDQGWVGWFCQKAVEIKNGINKPFTISGNGKQV 240
Y+ ++GL R ++YG GR + F + + G K + GK
Sbjct: 165 YSHLYGLPATGLRFFTVYGPWGRPDMALFK-----FTKA---MLEG--KSIDVYNYGKMK 214

Query: 241 RDVLHAEDMI-------SLYFTALANVSKIRGNA---------FNIGGTIVNSLSLLELF 284
RD + +D+ + A + G +NIG + + + L++
Sbjct: 215 RDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNS--SPVELMDYI 272

Query: 285 KLLEDYCNIDMRFTNLPVRESDQRVFVADIKKITNAIDWSPKVSAKDGVQKMYDW 339
+ LED I+ + LP++ D AD K + I ++P+ + KDGV+ +W
Sbjct: 273 QALEDALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNW 327


16SPA0813SPA0843Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0813-1163.483143hypothetical protein
SPA0814-1204.512334propionate kinase
SPA08150255.770481propanediol utilization protein PduV
SPA08161266.258271propanediol utilization protein PduU
SPA08171266.676313propanediol utilization protein PduT
SPA08182246.958389ferredoxin
SPA08193246.446243propanol dehydrogenase
SPA08203235.834545CoA-dependent proprionaldehyde dehydrogenase
SPA08213236.019431hypothetical protein
SPA08224215.299421Propanediol utilization: polyhedral bodies
SPA08231225.487091hypothetical protein
SPA08240214.836655hypothetical protein
SPA0825-1244.536084propanediol utilization protein PduK
SPA08260294.435353propanediol utilization protein PduJ
SPA08271304.918674PduH protein
SPA08281304.680476PduG protein
SPA08290231.484312diol dehydratase small subunit
SPA0830-2192.167619diol dehydratase medium subunit
SPA0831-2161.962008glycerol dehydratase large subunit
SPA0832-1151.479007propanediol utilization protein PduB
SPA0833-1150.968242propanediol utilization protein PduA
SPA0835-1181.282005pdu/cob regulatory protein PocR
SPA0837-1163.692094CbiB protein
SPA0838-1162.840541synthesis of vitamin B12 adenosyl cobalamide
SPA0839-2132.998934CbiD protein
SPA0840-3143.774087precorrin-6Y C5,15-methyltransferase
SPA0841-2133.302081precorrin-8W decarboxylase
SPA0842-2123.497854precorrin-4 C(11)-methyltransferase
SPA0843-1153.030227CbiG protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0814ACETATEKNASE5820.0 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 582 bits (1501), Expect = 0.0
Identities = 200/395 (50%), Positives = 279/395 (70%), Gaps = 5/395 (1%)

Query: 4 KIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHNQKWQETVPVADHRD 63
KI+ IN GSSSLK+QL+E G++L +GL ERIG+ D+ +T + +K + + DH+D
Sbjct: 2 KILVINCGSSSLKYQLIESKDGNVLAKGLAERIGINDSLLTHNANGEKIKIKKDMKDHKD 61

Query: 64 AVTLLLEKLLG--YQIINSLRDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAPL 121
A+ L+L+ L+ Y +I + +ID VGHRV HGGE+F S L+TD+ L I ELAPL
Sbjct: 62 AIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGEYFTSSVLITDDVLKAITDCIELAPL 121

Query: 122 HNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYIYPLPWHYYAELGIRRYGFHGT 181
HNP N GI Q++PD P VAVFDTAFHQT+ + AY+YP+P+ YY + IR+YGFHGT
Sbjct: 122 HNPANIEGIKACTQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRKYGFHGT 181

Query: 182 SHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTRS 241
SHKYVS AE L P+ +L++I CHLGNGSS+ A+KNG+S++TSMGFTP G+ MGTRS
Sbjct: 182 SHKYVSQRAAEILNKPIESLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGLAMGTRS 241

Query: 242 GDIDPSILPWIAQRESKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAA-NTGNRQAKLA 300
G IDPSI+ ++ ++E+ + +++ +LN +SG+ G+SG+SSD+RD+E AA G+++A+LA
Sbjct: 242 GSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRAQLA 301

Query: 301 LTLFAERIRATIGSYIMQMGGLDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQR 360
L +FA R++ TIGSY MGG+D +VFT GIGEN R + L+FLG +D+EKN+
Sbjct: 302 LNVFAYRVKKTIGSYAAAMGGVDVIVFTAGIGENGPEIREFILDGLEFLGFKLDKEKNKV 361

Query: 361 NA--TFIQTENALVKVAVINTNEELMIAQDVMRIA 393
I T ++ V V V+ TNEE MIA+D +I
Sbjct: 362 RGEEAIISTADSKVNVMVVPTNEEYMIAKDTEKIV 396


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0815SALSPVBPROT270.047 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 26.6 bits (58), Expect = 0.047
Identities = 11/24 (45%), Positives = 14/24 (58%)

Query: 93 IGLVTKADLADPQRISLVAQWLTQ 116
+G A L+DPQ S AQWL +
Sbjct: 171 LGKTAAARLSDPQAASHTAQWLVE 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0819BONTOXILYSIN300.014 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 30.3 bits (68), Expect = 0.014
Identities = 8/39 (20%), Positives = 16/39 (41%)

Query: 190 SDFIDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNAS 228
SDF ++ K LV+ +L + + + G +
Sbjct: 518 SDFSKVVSSKDKSLVYSFLDNLMSYLETIKNDGPIDTDK 556


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0825TONBPROTEIN280.019 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 27.6 bits (61), Expect = 0.019
Identities = 12/30 (40%), Positives = 15/30 (50%)

Query: 97 PPPSVIEPEPEESEIADVVSEAPAEEAPQE 126
PP V+EPEPE I + EAP +
Sbjct: 64 PPEPVVEPEPEPEPIPEPPKEAPVVIEKPK 93


17SPA0857SPA0871Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0857-128-3.212990hypothetical protein
SPA0861027-3.437038**hypothetical protein
SPA0862022-2.796673hypothetical protein
SPA0863021-1.757276hypothetical protein
SPA0864-120-1.416453AMP nucleosidase
SPA0866224-1.429134*hypothetical protein
SPA0867223-1.931075hypothetical protein
SPA0869220-1.964298*hypothetical protein
SPA0871220-1.268027*hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0864PF03627371e-04 PapG
		>PF03627#PapG

Length = 336

Score = 36.9 bits (85), Expect = 1e-04
Identities = 22/93 (23%), Positives = 34/93 (36%), Gaps = 8/93 (8%)

Query: 327 DDHVLDAVLPPDIP-------IPSIAEVQRALYDATKAVSGMPGEEVKQRLRTGTVVTTD 379
DD + LP D+P IP + +QR A +P K R ++
Sbjct: 152 DDIIFKVALPADLPLGDYSVTIPYTSGMQRHFASYLGARFKIPYNVAKTLPRENEMLFLF 211

Query: 380 DRNWELRYSASALRFNLSRAVAIDMESATIAAQ 412
R SA +L ++I+ + AAQ
Sbjct: 212 KNIGGCRPSAQSLEIKHGD-LSINSANNHYAAQ 243


18SPA0884SPA0898Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0884016-3.788943hypothetical protein
SPA0886016-4.396466hypothetical protein
SPA0887116-2.813016DsrB protein
SPA0888016-2.446033colanic acid capsullar biosynthesis activation
SPA08890130.443089flagellar biosynthetic protein FliR
SPA0890-1141.174655flagellar biosynthetic protein FliQ
SPA0891-1153.273445flagellar biosynthetic protein FliP
SPA08920143.210124flagellar protein FliO
SPA0893-2143.844192flagellar motor switch protein FliN
SPA0894-1174.568567flagellar motor switch protein FliM
SPA08951154.860523FliL protein
SPA08960134.834891flagellar hook-length control protein
SPA0897-1134.093197flagellar FliJ protein
SPA0898-2133.627967flagellum-specific ATP synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0889TYPE3IMRPROT2135e-71 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 213 bits (543), Expect = 5e-71
Identities = 231/260 (88%), Positives = 246/260 (94%)

Query: 1 MIQVTSEQWLYWLHLYFWPLLRVLALISTAPILSERAIPKRVKLGLGIMITLVIAPSLPA 60
M+QVTSEQWL WL+LYFWPLLRVLALISTAPILSER++PKRVKLGL +MIT IAPSLPA
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 NDTPLFSIAALWLAMQQILIGIALGFTMQFAFAAVRTAGEFIGLQMGLSFATFVDPGSHL 120
ND P+FS ALWLA+QQILIGIALGFTMQFAFAAVRTAGE IGLQMGLSFATFVDP SHL
Sbjct: 61 NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHL 120

Query: 121 NMPVLARIMDMLAMLLFLTFNGHLWLISLLVDTFHTLPIGSNPVNSNAFMALARAGGLIF 180
NMPVLARIMDMLA+LLFLTFNGHLWLISLLVDTFHTLPIG P+NSNAF+AL +AG LIF
Sbjct: 121 NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIF 180

Query: 181 LNGLMLALPVITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGIMLMAALMPLIAPFC 240
LNGLMLALP+ITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGI LMAALMPLIAPFC
Sbjct: 181 LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC 240

Query: 241 EHLFSEIFNLLADIVSEMPI 260
EHLFSEIFNLLADI+SE+P+
Sbjct: 241 EHLFSEIFNLLADIISELPL 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0890TYPE3IMQPROT671e-18 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 67.5 bits (165), Expect = 1e-18
Identities = 23/78 (29%), Positives = 42/78 (53%)

Query: 4 ESVMMMGTEAMKVALALAAPLLLVALITGLIISILQAATQINEMTLSFIPKIVAVFIAII 63
+ ++ G +A+ + L L+ +VA I GL++ + Q TQ+ E TL F K++ V + +
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 VAGPWMLNLLLDYVRTLF 81
+ W +LL Y R +
Sbjct: 62 LLSGWYGEVLLSYGRQVI 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0891FLGBIOSNFLIP328e-117 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 328 bits (842), Expect = e-117
Identities = 224/245 (91%), Positives = 232/245 (94%)

Query: 1 MRRLLFLSLAGLWLFSPAAAAQLPGLISQPLAGGGQSWSLSVQTLVFITSLTFLPAILLM 60
MRRLL ++ LWL +P A AQLPG+ SQPL GGGQSWSL VQTLVFITSLTF+PAILLM
Sbjct: 1 MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLM 60

Query: 61 MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEQK 120
MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSE+K
Sbjct: 61 MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEK 120

Query: 121 ISMQEALDKGAQPLCAFMLRQTREADLALFARLANSGPLQGPEAVPMRILLPAYVTSELK 180
ISMQEAL+KGAQPL FMLRQTREADL LFARLAN+GPLQGPEAVPMRILLPAYVTSELK
Sbjct: 121 ISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELK 180

Query: 181 TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA 240
TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA
Sbjct: 181 TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA 240

Query: 241 QSFYS 245
QSFYS
Sbjct: 241 QSFYS 245


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0893FLGMOTORFLIN2092e-73 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 209 bits (534), Expect = 2e-73
Identities = 136/137 (99%), Positives = 136/137 (99%)

Query: 1 MSDMNNPSDENTGALDDLWADALNEQKATTNKSAADAVFQQLGGGDVSGAMQDIDLIMDI 60
MSDMNNPSDENTGALDDLWADALNEQKATT KSAADAVFQQLGGGDVSGAMQDIDLIMDI
Sbjct: 1 MSDMNNPSDENTGALDDLWADALNEQKATTTKSAADAVFQQLGGGDVSGAMQDIDLIMDI 60

Query: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120
PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV
Sbjct: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120

Query: 121 RITDIITPSERMRRLSR 137
RITDIITPSERMRRLSR
Sbjct: 121 RITDIITPSERMRRLSR 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0894FLGMOTORFLIM383e-135 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 383 bits (984), Expect = e-135
Identities = 86/324 (26%), Positives = 148/324 (45%), Gaps = 10/324 (3%)

Query: 5 ILSQAEIDALLNGDS--DTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINERFA 62
+LSQ EID LL S D E I+ I YD + +E+++ L +++E FA
Sbjct: 4 VLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFA 63

Query: 63 RQFRMGLFNLLRRSPDITVGTIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSL 122
R L LR + V ++ Y EF R++P P+ L +I + PL+G ++ PS+
Sbjct: 64 RLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPSI 123

Query: 123 VFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYVRS 182
F +D LFGG G+ KV+ R+ T E V+ ++ L ++W + L +
Sbjct: 124 TFSIIDRLFGGTGQ-AAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQI 181

Query: 183 EMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENS--R 240
E +F I P+++VV ++G G N C+P+ IEP+ L + +S R
Sbjct: 182 ETNPQFAQI-VPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRR 240

Query: 241 HEDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKP---DRIIAHVD 297
+ L ++ ++++VA + L + IL L+ GD++ + D + +
Sbjct: 241 SSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIG 300

Query: 298 GVPVLTSQYGTVNGQYALRVEHLI 321
Q G V + A ++ I
Sbjct: 301 NRKKFLCQPGVVGKKIAAQILERI 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0896FLGHOOKFLIK406e-143 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 406 bits (1045), Expect = e-143
Identities = 193/411 (46%), Positives = 233/411 (56%), Gaps = 40/411 (9%)

Query: 1 MITLPQLITTDTDMTAGLTSGKTTGSAEDFLALLAGALGADGAQGKDARITLADLQAAGG 60
MI L LIT D D T L GK + +A+DFLALL+ AL + K A L
Sbjct: 1 MIRLAPLITADVDTTT-LPGGKASDAAQDFLALLSEALAGETTTDKAAPQLL-------- 51

Query: 61 KLSKELLTQHGEPGQAVKLADLLAQKAN---ATDETLTDLTQAQHLLSTLTLSLKTSALA 117
++ + T GEP + ++D AQ+AN DET + Q + LT + + A
Sbjct: 52 -VATDKPTTKGEPLISDIVSD--AQQANLLIPVDETPPVINDEQSTSTPLTTAQTMALAA 108

Query: 118 ALSKTAQHDEKTPALSDEDLASLSALFAMLPGQPVATPVAGETPAENHIALPSLLRGDMP 177
K DEK L+++ ASLSALFAMLPG V D P
Sbjct: 109 VADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVT-----------------DAP 151

Query: 178 SAPQEETHTLSFSEHEKGKTEASLARASDDRATGPALTPLVVAAAATSAKVEVDSPPAPV 237
S F++ T L A D A G PL A +K EV S P+PV
Sbjct: 152 STVLPTEKPTLFTK----LTSEQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPV 207

Query: 238 THGAAMPTLSSATAQPQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFTRQGQQSAQLRLH 297
T AA P ++ QP LP +APVLSAPLGSHEWQQ+ SQ + LFTRQGQQSA+LRLH
Sbjct: 208 T-AAASPLITPHQTQP--LPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLH 264

Query: 298 PEELGQVHISLKLDDNQAQLQMVSPHSHVRAALEAALPMLRTQLAESGIQLGQSSISSES 357
P++LG+V ISLK+DDNQAQ+QMVSPH HVRAALEAALP+LRTQLAESGIQLGQS+IS ES
Sbjct: 265 PQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGES 324

Query: 358 FAGQQQ-SSSQQQSSRAQHTDAFGAEDDIALAAPASLQAAARGNGAVDIFA 407
F+GQQQ +S QQQS R + + EDD L P SLQ GN VDIFA
Sbjct: 325 FSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0897FLGFLIJ2064e-72 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 206 bits (526), Expect = 4e-72
Identities = 130/147 (88%), Positives = 138/147 (93%)

Query: 1 MAQHGALETLKDLAEKEVDDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRSNLNTDMGNG 60
MA+HGAL TLKDLAEKEV+DAARLLGEMRRGCQQAEEQLKMLIDYQNEYR+NLN+DM G
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 IASNRWINYQQFIQTLEKAIEQHRLQLTQWTQKVDLALKSWREKKQRLQAWQTLQDRQTA 120
I SNRWINYQQFIQTLEKAI QHR QL QWTQKVD+AL SWREKKQRLQAWQTLQ+RQ+
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 AALLAENRMDQKKMDEFAQRAAMRKPE 147
AALLAENR+DQKKMDEFAQRAAMRKPE
Sbjct: 121 AALLAENRLDQKKMDEFAQRAAMRKPE 147


19SPA0992SPA1017Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0992224-1.178559hypothetical protein
SPA0993226-1.437708DNA polymerase III, theta subunit
SPA0994226-4.211724cation resistance protein
SPA0995233-8.470246cation transporter
SPA0996334-10.424614hypothetical protein
SPA0997435-10.462949hypothetical protein
SPA0999434-10.332829hypothetical protein
SPA1000533-8.955783hypothetical protein
SPA1001426-6.484830hypothetical protein
SPA1002623-4.366771phage integrase protein
SPA1003626-5.142185hypothetical protein
SPA1004625-5.750174hypothetical protein
SPA1005630-6.888108hypothetical protein
SPA1006530-7.712349hydrolase
SPA1007744-10.869786hypothetical protein
SPA1008542-10.227595hypothetical protein
SPA1009543-10.442502hypothetical protein
SPA1010441-8.918023hypothetical protein
SPA1011434-9.093882hypothetical protein
SPA1013536-9.902032hypothetical protein
SPA1014436-9.635305hypothetical protein
SPA1015233-7.850323acetyltransferase
SPA1016132-7.132080hypothetical protein
SPA1017032-7.529134hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1005ACRIFLAVINRP354e-04 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 35.2 bits (81), Expect = 4e-04
Identities = 25/83 (30%), Positives = 40/83 (48%), Gaps = 10/83 (12%)

Query: 123 QLPFAWPLSVILMLTALAALY--YHLPALLLFIVPLWLT-ALLASVQLNQYMNIRFLLVW 179
Q P +S +++ LAALY + +P ++ +VPL + LLA+ NQ ++ F++
Sbjct: 871 QAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGL 930

Query: 180 LTL------TAILIYGRFILQRW 196
LT AILI F
Sbjct: 931 LTTIGLSAKNAILIVE-FAKDLM 952


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1008PilS_PF08805290.013 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 28.7 bits (64), Expect = 0.013
Identities = 5/34 (14%), Positives = 13/34 (38%), Gaps = 2/34 (5%)

Query: 112 WTLITSI--LIIIAVAVVLAISSMNAAFRSLNIN 143
TL+ + + +I V A + ++ +
Sbjct: 28 ATLMEVLLVVGVIVVLAASAYKLYSMVQSNIQSS 61


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1015SACTRNSFRASE280.008 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.4 bits (63), Expect = 0.008
Identities = 13/60 (21%), Positives = 28/60 (46%), Gaps = 2/60 (3%)

Query: 60 WLCIDYLWVSESARSRGLGSQLMEMAEKEGLRKGCVHGLVDTFSFQ--ALPFYEKQGYIL 117
+ I+ + V++ R +G+G+ L+ A + +++T A FY K +I+
Sbjct: 89 YALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


20SPA1142SPA1153Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1142-115-3.200916intracellular septation protein
SPA1143-120-3.851863hypothetical protein
SPA1144-123-4.212643hypothetical protein
SPA1145020-2.438909outer membrane protein
SPA1146218-1.153336hypothetical protein
SPA1147217-0.419999hypothetical protein
SPA11481151.750062hypothetical protein
SPA1149-1143.337026hypothetical protein
SPA1150-1133.413644tryptophan synthase subunit alpha
SPA1151-1122.964629tryptophan synthase subunit beta
SPA1152-2102.951873indole-3-glycerol phosphate synthase
SPA1153-2103.041514anthranilate synthase component II; anthranilate
21SPA1179SPA1194Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1179-120-3.298447hypothetical protein
SPA1180021-2.998168peptide transport system ATP-binding protein
SPA1181128-4.796025peptide transport system ATP-binding protein
SPA1182435-6.562720peptide transport system permease SapC
SPA1183432-7.803816integrase
SPA1186123-4.668562hypothetical protein
SPA1187017-1.734052hypothetical protein
SPA1188015-1.830376hypothetical periplasmic protein
SPA1190114-0.564887hypothetical protein
SPA1191114-0.136328peptide transport system permease SapB
SPA11923130.195038peptide transport periplasmic protein SapA
SPA1193316-1.141449psp operon transcriptional activator PspF
SPA1194218-0.788707phage shock protein A
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1181HTHFIS310.007 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.007
Identities = 9/16 (56%), Positives = 14/16 (87%)

Query: 38 LVGESGSGKSLIAKAI 53
+ GESG+GK L+A+A+
Sbjct: 165 ITGESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1193HTHFIS341e-117 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 341 bits (877), Expect = e-117
Identities = 123/345 (35%), Positives = 177/345 (51%), Gaps = 22/345 (6%)

Query: 2 AEFKDNLLGEANRFLEVLEQVSRLAPLDKPVLIIGERGTGKELIANRLHYLSSRWQGPLI 61
++ L+G + E+ ++RL D ++I GE GTGKEL+A LH R GP +
Sbjct: 133 SQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFV 192

Query: 62 SLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGGTLFLDELATAPMLVQEKLL 121
++N AA+ +L++SELFGHE GAFTGAQ R GRFE+A+GGTLFLDE+ PM Q +LL
Sbjct: 193 AINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLL 252

Query: 122 RVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVKKGTFRADLLDRLAFDVVQLPPLRE 181
RV++ GE VGG P++ +VR+V ATN DL + +G FR DL RL ++LPPLR+
Sbjct: 253 RVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRD 312

Query: 182 RQSDTMLMAEHFAIQMCRELRLPLFPGFTDRAKETLLHYAWPGNVRELKNVVERSVYRHG 241
R D + HF Q +E F A E + + WPGNVREL+N+V R +
Sbjct: 313 RAEDIPDLVRHFVQQAEKEGLDVK--RFDQEALELMKAHPWPGNVRELENLVRRLTALYP 370

Query: 242 SSE--------HPLDEIVIDPFQRHPAEPPTPALPSA------------SATPDLPLNLR 281
EI P ++ A + ++ A
Sbjct: 371 QDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYD 430

Query: 282 EFQLQQEKALLQRSLQQAKFNQKRAADLLALTYHQFRALLKKHQL 326
+ E L+ +L + NQ +AADLL L + R +++ +
Sbjct: 431 RVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGV 475


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1194RTXTOXIND290.019 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.6 bits (64), Expect = 0.019
Identities = 19/104 (18%), Positives = 43/104 (41%), Gaps = 5/104 (4%)

Query: 40 LVEVRSNSARALAEKKQLSRRIEQATAQQTEWQEKAELA-LRKDKDDLARAALIEKQKLT 98
+ + R + +L K+ +++ + + EL + + + L K++
Sbjct: 232 VEKSRLDDFSSLLHKQAIAK-HAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQ 290

Query: 99 DLIATLEQEVTLVDDTLARMKKEIGELENKLSETRARQQALMLR 142
+ + E+ D L + IG L +L++ RQQA ++R
Sbjct: 291 LVTQLFKNEIL---DKLRQTTDNIGLLTLELAKNEERQQASVIR 331


22SPA1207SPA1232Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1207-119-3.314505transcriptional regulator
SPA1208024-4.749328oxidoreductase
SPA1209330-6.164827oxidoreductase
SPA1210435-8.526066transcriptional regulator
SPA1211538-10.657549lipoprotein
SPA1213335-8.982905transcriptional regulator
SPA1214231-6.612920lipoprotein
SPA1216026-7.303658hypothetical protein
SPA1217-220-5.694073thiol peroxidase
SPA1218-117-4.061225hypothetical protein
SPA1219-114-3.431911hypothetical protein
SPA1220013-3.326314DNA-binding protein
SPA1221-113-3.240812hypothetical protein
SPA1222014-1.319139hypothetical protein
SPA1223-112-0.148802hypothetical protein
SPA1224-111-0.528263fumarate and nitrate reduction regulatory
SPA1225023-4.031067O6-methylguanine-DNA-alkyltransferase
SPA1226023-4.954507hypothetical protein
SPA1228024-5.353655membrane transport protein
SPA1230-215-2.829418ATP-dependent RNA helicase, stimulated by 23S
SPA1231-117-3.653165hypothetical protein
SPA1232-217-3.549062hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1209DHBDHDRGNASE871e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 86.6 bits (214), Expect = 1e-22
Identities = 67/249 (26%), Positives = 112/249 (44%), Gaps = 24/249 (9%)

Query: 7 KSVLVLGGSRGIGAAIVRRFSADGASVV-FSYAGSR----EAAEKLAAETGSTAIQTDSA 61
K + G ++GIG A+ R ++ GA + Y + ++ K A + A D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARH-AEAFPADVR 67

Query: 62 DRDAVISLV----REYGPLDILVVNAGVALFGDALEQDSDAIDRLFRINIHAPYHASVEA 117
D A+ + RE GP+DILV AGV G + + F +N ++AS
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 118 ARNMP--EGGRIIIIGSVNGDRMPVPGMAAYAASKSALQGLARGLARDFGPRGITINVVQ 175
++ M G I+ +GS N +P MAAYA+SK+A + L + I N+V
Sbjct: 128 SKYMMDRRSGSIVTVGS-NPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 176 PGPIDTDI--------NPEDGPMKELMHSF---MAIKRHGRPKEVAGMVAWLAGPEASFV 224
PG +TD+ N + +K + +F + +K+ +P ++A V +L +A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 225 TGAMHTIDG 233
T +DG
Sbjct: 247 TMHNLCVDG 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1210HTHTETR453e-08 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 45.4 bits (107), Expect = 3e-08
Identities = 14/115 (12%), Positives = 39/115 (33%), Gaps = 5/115 (4%)

Query: 6 SRTPGRPRQFDPEQAIKTAQHLFHSRGYDAVSVADLTKAFGINPPSFYAAFGSKLGLYTR 65
+T ++ + + A LF +G + S+ ++ KA G+ + Y F K L++
Sbjct: 3 RKTKQEAQE-TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 66 VLK----RYRMTDAIPLGALLRHDRPTAKCLIDVLMEAARRYAADPDATGCLVLE 116
+ + + + ++ ++E+ + +
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHK 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1211adhesinb270.006 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 27.1 bits (60), Expect = 0.006
Identities = 9/48 (18%), Positives = 18/48 (37%), Gaps = 6/48 (12%)

Query: 1 MQKCSLITVISLSVLMLAGCTTTYTMTTRTGEIIETQGKPEVDTATGM 48
M+KC + ++ L+ + LA C++ K V +
Sbjct: 1 MKKCRFLVLLLLAFVGLAACSSQ------KSSTETGSSKLNVVATNSI 42


23SPA1301SPA1325Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1301-115-3.643051alcohol dehydrogenase
SPA1302018-5.058280NAD-linked malic enzyme; malate oxidoreductase
SPA1303225-6.40563830S ribosomal protein S22
SPA1304120-3.619910hypothetical protein
SPA1305-116-1.858042osmotically inducible protein C
SPA1306-114-1.736161hemolysin HlyE
SPA1308-114-0.417238hypothetical protein
SPA1309-116-1.459092hypothetical protein
SPA1310-117-1.745124hydrolase
SPA1311018-2.286195hydrolase
SPA1312118-3.123102glycogen debranching protein
SPA1313019-3.638433aminotransferase
SPA1315018-3.687444hypothetical protein
SPA1316014-1.526482regulatory protein
SPA1317013-1.626901uptake hydrogenase small subunit
SPA1318-114-1.377693hydrogenase-1 large subunit
SPA1319018-1.636629Ni/Fe-hydrogenase 1 b-type cytochrome subunit
SPA1320021-2.877249hydrogenase 1 maturation protease
SPA1322023-3.442650hydrogenase-1 operon protein HyaE2
SPA1323024-4.464058hydrogenase-1 operon protein HyaF2
SPA1324025-4.362467ATP/GTP-binding protein
SPA1325020-4.154991hydrogenase
24SPA1425SPA1472Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1425019-4.860052integral membrane transport protein
SPA1426022-6.559204cyclopropane-fatty-acyl-phospholipid synthase
SPA1427127-7.576303riboflavin synthase subunit alpha
SPA1428334-8.218265hypothetical protein
SPA1431545-11.122350**type III secretion protein
SPA1432544-10.195947type III secretion protein
SPA1433341-6.802426type III secretion protein
SPA1434235-6.615353type III secretion protein
SPA1435235-6.460463type III secretion protein
SPA1436134-6.520073type III secretion protein
SPA1437034-6.858910type III secretion protein
SPA1438036-6.682319type III secretion ATP synthase
SPA1439137-8.397770Secretion system apparatus
SPA1440342-9.401519pathogenicity island protein
SPA1441243-9.227740secretion system protein
SPA1442441-10.242857pathogenicity island protein
SPA1443643-8.806166pathogenicity island protein
SPA1444644-7.957803pathogenicity island lipoprotein
SPA1445842-6.580193pathogenicity island protein
SPA1446743-6.710277pathogenicity island protein
SPA1447641-6.004158pathogenicity island protein
SPA1448438-6.205200pathogenicity island effector protein
SPA1449336-6.063866pathogenicity island effector protein
SPA1450332-7.004349pathogenicity island protein
SPA1451335-7.671917pathogenicity island effector protein
SPA1452434-7.685469pathogenicity island effector protein
SPA1453437-8.918023pathogenicity island effector protein
SPA1454540-9.985365Type III secretion system chaperone protein
SPA1455743-10.940354pathogenicity island effector effector protein
SPA1456442-10.853540pathogenicity island protein
SPA1457442-11.025728secretion system protein
SPA1458439-9.834355pathogenicity island protein
SPA1459233-8.092680outer membrane secretory protein
SPA1460231-7.060329pathogenicity island secreted effector protein
SPA1461028-5.767530two-component sensor kinase
SPA14620170.190781two-component response regulator
SPA14630142.180498transcriptional regulator
SPA1464-1123.093163pathogenicity island protein
SPA14650131.683158hypothetical protein
SPA14660141.328299two-component response regulator
SPA1467-1130.657407hypothetical protein
SPA1469-113-0.169979tetrathionate reductase subunit C (membrane
SPA1470-214-0.504955tetrathionate reductase subunit A
SPA1472-219-3.678085hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1425TCRTETB763e-17 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 76.1 bits (187), Expect = 3e-17
Identities = 48/194 (24%), Positives = 84/194 (43%), Gaps = 3/194 (1%)

Query: 8 LVWLAGLSVLGFLATDMYLPAFAAIQADLQTPAAAVSASLSLFLAGFAVAQLLWGPLSDR 67
L+WL LS L + + I D P A+ + + F+ F++ ++G LSD+
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 68 YGRKPILLLGLSIFALGSLGMLWVESAAALLTL-RFVQAVGVCAATVIWQALVTDYYPSQ 126
G K +LL G+ I GS+ S +LL + RF+Q G A + +V Y P +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 127 KINRIFATIMPLVGLSPALAPLLGSWILTHFSWQAIFATLFVITLLLMLPALRLKPSVKA 186
+ F I +V + + P +G I + W + L + ++ +P L +
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPMITIITVPFLMKLLKKEV 193

Query: 187 RTEGQDKLTFATLL 200
R +G + L+
Sbjct: 194 RIKGHFDIKGIILM 207


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1431TYPE3IMSPROT386e-136 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 386 bits (992), Expect = e-136
Identities = 125/350 (35%), Positives = 203/350 (58%), Gaps = 4/350 (1%)

Query: 2 SEKTEQPTEKKLRDGRKEGQVVKSIEITSLFQLIALYLYFHFFTEKMILILIESITFTLQ 61
EKTEQPT KK+RD RK+GQV KS E+ S ++AL ++ + + +
Sbjct: 3 GEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAE 62

Query: 62 LVNKPFSYALTQL-SHALIESLTSALLFLGAGVIVATVGSVFLQVGVVIASKAIGFKSEH 120
PFS AL+ + + L+E L ++A + S +Q G +I+ +AI +
Sbjct: 63 QSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMA-IASHVVQYGFLISGEAIKPDIKK 121

Query: 121 INPVSNFKQIFSLHSVVELCKSSLKVIMLSLIFAFFFYYYASTFRALPYCGLACGVLVVS 180
INP+ K+IFS+ S+VE KS LKV++LS++ T LP CG+ C ++
Sbjct: 122 INPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLG 181

Query: 181 SLIKWLWVGVMAFYIVVGILDYSFQYYKIRKDLKMSKDDVKQEHKDLEGDPQMKTRRREM 240
+++ L V ++V+ I DY+F+YY+ K+LKMSKD++K+E+K++EG P++K++RR+
Sbjct: 182 QILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQF 241

Query: 241 QSEIQSGSLAQSVKQSVAVVRNPTHIAVCLGYHPTDMPIPRVLEKGSDAQANYIVNIAER 300
EIQS ++ ++VK+S VV NPTHIA+ + Y + P+P V K +DAQ + IAE
Sbjct: 242 HQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEE 301

Query: 301 NCIPVVENVELARSLFFEVERGDKIPETLFEPVAALLRMVMK--IDYAHS 348
+P+++ + LAR+L+++ IP E A +LR + + I+ HS
Sbjct: 302 EGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNIEKQHS 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1432TYPE3IMRPROT1644e-52 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 164 bits (417), Expect = 4e-52
Identities = 55/229 (24%), Positives = 100/229 (43%), Gaps = 5/229 (2%)

Query: 8 WLIALAVAFIRPLSLSLLLPLLKSGSLGSAILRNGVLMSLTFPILPIIYQQKIMMHIGKD 67
WL +R L+L P+L S+ + + G+ M +TF I P + + +
Sbjct: 12 WLNLYFWPLLRVLALISTAPILSERSVPKRV-KLGLAMMITFAIAPSLPANDVPVF---S 67

Query: 68 YSWLGLVTGEVIIGFLIGFCAAVPFWAVDMAGFLLDTLRGATMGTIFNSTMEAETSLFGL 127
+ L L +++IG +GF F AV AG ++ G + T + +
Sbjct: 68 FFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLAR 127

Query: 128 LFSQFLCVIFFISGGVEFILNILYESYQYLPPGRTLLFDRQFLKYIQAEWRTLYQLCISF 187
+ ++F G +++++L +++ LP G L FL +A ++ +
Sbjct: 128 IMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSL-IFLNGLML 186

Query: 188 SLPAIICMVLADLALGLLNRSAQQLNVFFFSMPLKSILVLLTLLISFPY 236
+LP I ++ +LALGLLNR A QL++F PL + + + P
Sbjct: 187 ALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPL 235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1433TYPE3IMQPROT729e-21 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 72.5 bits (178), Expect = 9e-21
Identities = 30/85 (35%), Positives = 50/85 (58%)

Query: 4 SELTQFVTQLLWIVLFTSMPVVLVASVVGVIVSLVQALTQIQDQTLQFMIKLLAIAITLM 63
+L + L++VL S +VA+++G++V L Q +TQ+Q+QTL F IKLL + + L
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 VSYPWLSGILLNYTRQIMLRIGEHG 88
+ W +LL+Y RQ++ G
Sbjct: 62 LLSGWYGEVLLSYGRQVIFLALAKG 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1434TYPE3IMPPROT2319e-80 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 231 bits (592), Expect = 9e-80
Identities = 79/215 (36%), Positives = 130/215 (60%), Gaps = 8/215 (3%)

Query: 8 LQLIGILFLLSILPLIIVMGTSFLKLAVVFSILRNALGIQQVPPNIALYGLALVLSLFIM 67
+ LI +L ++LP II GT F+K ++VF ++RNALG+QQ+P N+ L G+AL+LS+F+M
Sbjct: 5 ISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLSMFVM 64

Query: 68 GPTLLAVKERWHPVQVAGAPFWT-SEWDSKALAPYRQFLQKNSEEKEANYFRNLIKRTWP 126
P + + V + S+ + L YR +L K S+ + +F N +
Sbjct: 65 WPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLIKYSDRELVQFFENAQLKRQY 124

Query: 127 ED-------IKRKIKPDSLLILIPAFTVSQLTQAFRIGLLIYLPFLAIDLLISNILLAMG 179
+ K +I+ S+ L+PA+ +S++ AF+IG +YLPF+ +DL++S++LLA+G
Sbjct: 125 GEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVLLALG 184

Query: 180 MMMVSPMTISLPFKLLIFLLAGGWDLTLAQLVQSF 214
MMM+SP+TIS P KL++F+ GW L L+ +
Sbjct: 185 MMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQY 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1435TYPE3OMOPROT542e-10 Type III secretion system outer membrane O protein ...
		>TYPE3OMOPROT#Type III secretion system outer membrane O protein

family signature.
Length = 303

Score = 53.9 bits (129), Expect = 2e-10
Identities = 59/291 (20%), Positives = 96/291 (32%), Gaps = 33/291 (11%)

Query: 31 QYPVQQGTLFTINYHNELGRVWIAEQCWQRWCEGLIGTANRSAIDPELLYGIAEWGVAPL 90
+YP +QG ++ + WI W + A SA AE V P
Sbjct: 32 EYPTRQGMWVRLSDAEKRWSAWIKPGDWLEHVSPALAGAAVSAG--------AEHLVVPW 83

Query: 91 LQASDATLCQNEPPTSCSNLPHQLALHIKWTVEEHEFHSIIFTWPTGFLRNIVGELSAER 150
L A++ P SC L VE S + P G L +I+ +
Sbjct: 84 LAATERPFELPVPHLSCRRL----------CVENPVPGSAL---PEGKLLHIMSDRGGLW 130

Query: 151 QQIYPAPPVVVPVYLGWCQLTLIELESIEIGMG-VRIHCFGDIRLGFFAIQLPGGIYARV 209
+ P P V L IG + G I +G + L A V
Sbjct: 131 FEHLPELPAVGGGRPK----MLRWPLRFVIGSSDTQRSLLGRIGIG--DVLLIRTSRAEV 184

Query: 210 LLTEDNTMKFDELVQDIETLLASGSPMSKSDGTSSV-----ELEQIPQQVLFEIGRASLE 264
F+ + I + + + T+ L Q+P ++ F + R ++
Sbjct: 185 YCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYRKNVT 244

Query: 265 IGQLRQLKTGDVLPVGGCFAPEVTIRVNDRIIGQGELIACGNEFMVRITRW 315
+ +L + +L + V I N ++G GEL+ + V I W
Sbjct: 245 LAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEW 295


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1444FLGMRINGFLIF525e-10 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 52.3 bits (125), Expect = 5e-10
Identities = 29/183 (15%), Positives = 70/183 (38%), Gaps = 15/183 (8%)

Query: 23 LYRSLPEDEANQMLALLMQHHIDAEKKQEEDGVTLRVEQSQFINAVELLRLNGYPHRQFT 82
L+ +L + + ++A L Q +I + V + L G P +
Sbjct: 53 LFSNLSDQDGGAIVAQLTQMNIPYRFA--NGSGAIEVPADKVHELRLRLAQQGLP-KGGA 109

Query: 83 TADKMFPANQLVVSPQEEQQKINFLK--EQRIEGMLSQMEGVINAKVTIALPTYDEGS-- 138
++ + +S EQ +N+ + E + + + V +A+V +A+P + S
Sbjct: 110 VGFELLDQEKFGISQFSEQ--VNYQRALEGELARTIETLGPVKSARVHLAMP---KPSLF 164

Query: 139 --NASPSSVAVFIKYSPQVNMEAFRVK-IKDLIEMSIPGLQYSKISILMQPAEFRMVADV 195
S +V + P ++ ++ + L+ ++ GL ++++ Q ++
Sbjct: 165 VREQKSPSASVTVTLEPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNT 224

Query: 196 PAR 198
R
Sbjct: 225 SGR 227


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1450SYCDCHAPRONE775e-21 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 77.3 bits (190), Expect = 5e-21
Identities = 26/127 (20%), Positives = 49/127 (38%)

Query: 14 LKQLLSVDPETVYASGYASWQEGDYSRAVIDFSWLVMAQPWSWRAHIALAGTWMMLKEYT 73
L ++ S E +Y+ + +Q G Y A F L + + R + L + +Y
Sbjct: 28 LNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYD 87

Query: 74 TAINFYGHALMLDASHPEPVYQTGVCLKMMGEPGLAREAFQTAIKMSYADASWSEIRQNA 133
AI+ Y + ++D P + CL GE A A ++ + E+
Sbjct: 88 LAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRV 147

Query: 134 QKMVDTL 140
M++ +
Sbjct: 148 SSMLEAI 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1452PF05844290.010 YopD protein
		>PF05844#YopD protein

Length = 295

Score = 29.2 bits (65), Expect = 0.010
Identities = 29/149 (19%), Positives = 48/149 (32%), Gaps = 19/149 (12%)

Query: 9 VLPAPSL-LTPSSTPSPSGEGMGTESMLLLFDDIWMKLMELAKKLRDIMRSYNVEKQRLS 67
L AP L P + E + +LL+ I K EL RD + Q+
Sbjct: 50 ELNAPRQVLDPVRMEAAGSELDSSVELLLILFRIAQKARELGVLQRDNENQAIIHAQK-- 107

Query: 68 WELQVNVLQTQMKTIDEAFRASMITAGGAMLSGVLTIGLGAVGGETGLIAGQAVGHTAGG 127
+DE + + A+++GV + VG L G+A+
Sbjct: 108 ------------AQVDEMRSGATLMIAMAVIAGVGALASAVVGSLGALKNGKAISQEK-- 153

Query: 128 VMGLGSGVAQRQSDQDKAIADLQQNGAQS 156
L + R D + L + +
Sbjct: 154 --TLQKNIDGRNELIDAKMQALGKTSDED 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1454SYCDCHAPRONE902e-25 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 89.6 bits (222), Expect = 2e-25
Identities = 39/154 (25%), Positives = 67/154 (43%), Gaps = 7/154 (4%)

Query: 6 TLQQAHDTMRFFRRGGSLRMLL---DDDVTQPLNTVYRYAMQLMEVKEFAGAARLFQLLT 62
T + F + GG++ ML D L +Y A + ++ A ++FQ L
Sbjct: 8 TQEYQLAMESFLKGGGTIAMLNEISSDT----LEQLYSLAFNQYQSGKYEDAHKVFQALC 63

Query: 63 IYDAWSFDYWFRLGECCQAQKHWGEAIYAYGRAAQIKIDAPQAPWAAAECYLACDNVCYA 122
+ D + ++ LG C QA + AI++Y A + I P+ P+ AAEC L + A
Sbjct: 64 VLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEA 123

Query: 123 IKALKAVVRICGEVSEHQILRLRAEKMLQQLSDR 156
L + + +E + L R ML+ + +
Sbjct: 124 ESGLFLAQELIADKTEFKELSTRVSSMLEAIKLK 157


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1455LIPPROTEIN48270.047 Mycoplasma P48 major surface lipoprotein signature.
		>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature.

Length = 428

Score = 27.3 bits (60), Expect = 0.047
Identities = 15/44 (34%), Positives = 22/44 (50%)

Query: 78 SNEMDEVIAKAAKGDAKTKEEVPEDVIKYMRDNGILIDGMTIDD 121
+ E I K K +E+PED +KY+ + L DG ID+
Sbjct: 368 NTEEQAKINNKIKEAIKMFKELPEDFVKYINSDKALKDGNKIDN 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1459TYPE3OMGPROT5810.0 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 581 bits (1499), Expect = 0.0
Identities = 157/500 (31%), Positives = 260/500 (52%), Gaps = 15/500 (3%)

Query: 11 LLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLITATFSGKIPP 70
LL + + + + EL W + A+ L ++L NYD + +S I SG+
Sbjct: 17 LLLLSSYSWAQELDWLPIPYVYVAKGESLRDLLTDFGANYDATVVVSDKINDKVSGQFEH 76

Query: 71 GPPVDILNNLAAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIHYLRSQNILSS 130
P D L ++A+ Y+L+ ++DG++LY++ S + ++I L+ I
Sbjct: 77 DNPQDFLQHIASLYNLVWYYDGNVLYIFKNSEVASRLIRLQESEAAELKQALQRSGIWE- 135

Query: 131 PGCEVKEITGTKAVEVSGVPSCLTRISQLASVLDNALIKR--KDSAVSVSIYTLKYATAM 188
P + + V VSG P L + Q A+ L+ R K A+++ I+ LKYA+A
Sbjct: 136 PRFGWRPDASNRLVYVSGPPRYLELVEQTAAALEQQTQIRSEKTGALAIEIFPLKYASAS 195

Query: 189 DTQYQYRDQSVVVPGVVSVL-REMSKTSVPASSTTN-----GSPATQALPMFAADPRQNA 242
D YRD V PGV ++L R +S ++ + N + A ADP NA
Sbjct: 196 DRTIHYRDDEVAAPGVATILQRVLSDATIQQVTVDNQRIPQAATRASAQARVEADPSLNA 255

Query: 243 VIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGG---- 298
+IVRD M Y++LI LD+ IE+++ I+D+NA + +LG+DW + G
Sbjct: 256 IIVRDSPERMPMYQRLIHALDKPSARIEVALSIVDINADQLTELGVDWRVGIRTGNNHQV 315

Query: 299 --KKIAFNTGLNDGGASGFSTVISDTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVL 356
K + + GA G + R+N LE A V+S+P+++T N QAV+
Sbjct: 316 VIKTTGDQSNIASNGALGSLVDARGLDYLLARVNLLENEGSAQVVSRPTLLTQENAQAVI 375

Query: 357 DKNITFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIMLNLNIQDGQQSDTQSET 416
D + T+Y K+ G++VA+L+ IT G++LR+TPR+L +I LNL+I+DG Q S
Sbjct: 376 DHSETYYVKVTGKEVAELKGITYGTMLRMTPRVLTQGDKSEISLNLHIEDGNQKPNSSGI 435

Query: 417 DPLPEVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQV 476
+ +P + + + + A + GQSL++GG + + + +K+PLLGDIP +G LFR +
Sbjct: 436 EGIPTISRTVVDTVARVGHGQSLIIGGIYRDELSVALSKVPLLGDIPYIGALFRRKSELT 495

Query: 477 HSVIRLFLIKASVVNNGISH 496
+RLF+I+ +++ GI+H
Sbjct: 496 RRTVRLFIIEPRIIDEGIAH 515


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1461HTHFIS681e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.5 bits (165), Expect = 1e-13
Identities = 31/156 (19%), Positives = 57/156 (36%), Gaps = 13/156 (8%)

Query: 691 ILLVDDADINRDIIGKMLVSQGQHVTIAASSNEALTLSQQQRFDLVLIDIRMPEIDGIEC 750
IL+ DD R ++ + L G V I +++ DLV+ D+ MP+ + +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 751 VQLWHDEPNNLDPDCMFVALSASVATEDIHRCKKNGIHHYITKPVTLATLARYISIAAEY 810
+ PD + +SA + + G + Y+ KP L L
Sbjct: 66 LP----RIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG-------- 113

Query: 811 QLLRNIELQEQDPSRCSALLAT-DDMVINSKIFQSL 845
+ R + ++ PS+ +V S Q +
Sbjct: 114 IIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEI 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1462HTHFIS667e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 7e-15
Identities = 28/119 (23%), Positives = 50/119 (42%), Gaps = 2/119 (1%)

Query: 1 MKEYKILLVDDHEIIINGIMNALLPWPHFKIVEHVKNGLEVYNACCAYEPDILILDLSLP 60
M IL+ DD I + AL + V N ++ A + D+++ D+ +P
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGY--DVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 GINGLDIIPQLHQRWPAMNILVYTAYQQEYMTIKTLAAGANGYVLKSSSQQVLLAALQT 119
N D++P++ + P + +LV +A IK GA Y+ K L+ +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1466HTHFIS842e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 84.5 bits (209), Expect = 2e-21
Identities = 31/127 (24%), Positives = 56/127 (44%)

Query: 2 ATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMRMPVLDGQ 61
ATI + DDD A+ L GYDV+ + A + +V+ D+ MP +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 GVHDALRQCGSTLAVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALERALTVSS 121
+ +++ L V+ ++ A++ ++GA D+L KP + L + RAL
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 122 AAVARRE 128
++ E
Sbjct: 124 RRPSKLE 130


25SPA1499SPA1514Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1499220-0.624562hypothetical protein
SPA1500-3120.555855lipoprotein
SPA1501-3140.928045vitamin B12 ABC transport ATP-binding protein
SPA1502-3160.323258glutathione peroxidase/vitamin B12 transport
SPA1503-2180.573785vitamin B12 transport system permease
SPA1504121-1.286995integration host factor subunit alpha
SPA1505119-1.635635phenylalanyl-tRNA synthetase subunit beta
SPA1506025-6.734005phenylalanyl-tRNA synthetase subunit alpha
SPA1507030-8.74604750S ribosomal protein L20
SPA1508126-8.02348450S ribosomal subunit protein L35
SPA1510126-8.970588translation initiation factor IF-3
SPA1511126-8.753278threonyl-tRNA synthetase
SPA1512434-10.315647O-antigen polymerase
SPA1513124-4.162067hypothetical protein
SPA1514022-3.582731DNA/RNA non-specific endonuclease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1501PF05272300.014 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.014
Identities = 10/22 (45%), Positives = 13/22 (59%)

Query: 28 ILHLVGPNGAGKSTLLARMAGL 49
+ L G G GKSTL+ + GL
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1504DNABINDINGHU1196e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 119 bits (299), Expect = 6e-39
Identities = 34/89 (38%), Positives = 55/89 (61%)

Query: 4 TKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQRPGR 63
K ++ + + L+K+D+ V+ F + L GE+V+L GFGNF++R++ R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 64 NPKTGEDIPITARRVVTFRPGQKLKSRVE 92
NP+TGE+I I A +V F+ G+ LK V+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAVK 91


26SPA1527SPA1544Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1527-115-4.315708hypothetical protein
SPA1528014-5.048845phospho-beta-glucosidase B
SPA1529014-4.006350cel operon repressor
SPA1530115-1.352663phosphoenolpyruvate dependent phosphotransferase
SPA1531215-1.347294PTS system cellobiose-specific transporter
SPA15322150.608275PTS system cellobiose-specific transporter
SPA15331161.780802osmotically inducible lipoprotein E precursor
SPA15340173.000258NH3-dependent NAD synthetase
SPA15350173.312271hypothetical protein
SPA15360163.380652hypothetical protein
SPA15370163.320232succinylglutamate desuccinylase
SPA15380153.033158succinylarginine dihydrolase
SPA1539-1152.905421succinylglutamic semialdehyde dehydrogenase
SPA15400132.395465arginine succinyltransferase
SPA15410112.558476succinylornithine transaminase
SPA15420112.701253exodeoxyribonuclease III
SPA15430123.291662MutT-family protein
SPA15440133.037932hypothetical protein
27SPA1555SPA1611Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1555-112-3.022331aldose 1-epimerase
SPA1556-212-3.010642oxidoreductase
SPA1557-212-4.024657arylsulfatase regulator
SPA1558013-3.394998outer membrane protein
SPA1560-216-2.707820hypothetical protein
SPA1561-117-0.959570methyl-accepting chemotaxis protein
SPA15620172.075821hypothetical protein
SPA15630212.349583inner membrane protein
SPA15641222.612376hypothetical protein
SPA15652211.747371transcriptional regulator
SPA15662220.429002hypothetical protein
SPA1567130-3.186499bacteriophage protein
SPA1568330-3.526941hypothetical protein
SPA1569229-4.455800hypothetical protein
SPA1570226-5.705526hypothetical protein
SPA1571026-5.609438hypothetical protein
SPA1573124-5.833800hypothetical protein
SPA1574025-7.810033hypothetical protein
SPA1575027-7.859255hypothetical protein
SPA1576033-10.302790membrane transport protein
SPA1577131-8.279212chorismate mutase
SPA1578130-8.563197hypothetical protein
SPA1579228-7.279784hypothetical protein
SPA1580024-6.170938transcriptional regulator
SPA1581-123-4.512736regulatory protein
SPA1582019-1.614215aminoglycoside-resistance protein
SPA1583119-1.183515hypothetical protein
SPA1585117-0.033443*hypothetical protein
SPA15861150.058276hypothetical protein
SPA15872170.399716ABC transporter ATP-binding protein
SPA1588317-0.680021ABC transporter ATP-binding protein
SPA1589218-1.519105inner membrane transport protein
SPA1590119-2.600935inner membrane transport protein
SPA1591121-4.272429substrate-binding transport protein
SPA1592431-6.995426lipoprotein
SPA1593328-7.489451cytochrome
SPA1594432-8.267039hypothetical protein
SPA1596738-11.113631hypothetical protein
SPA1599639-10.574785*outer membrane invasion protein
SPA1600639-10.048703hypothetical protein
SPA1601645-9.234192hypothetical protein
SPA1602745-9.192710outer membrane virulence protein
SPA1603643-8.720930cold shock protein
SPA1604745-10.159487lipoprotein
SPA1605750-11.069596virulence protein
SPA1606752-12.302202toxin-like protein
SPA1607337-10.186791hypothetical protein
SPA1608133-8.417459hypothetical protein
SPA1609029-8.024704pertussis-like toxin subunit
SPA1610-223-6.163785pertussis-like toxin subunit
SPA1611-113-3.797602bacteriophage protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1564PRTACTNFAMLY280.012 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 28.5 bits (63), Expect = 0.012
Identities = 17/59 (28%), Positives = 25/59 (42%)

Query: 49 QGLTVGIIILTIGVMAPIASGTLPPSTLIHSFVNWKSLVAIAVGVFVSWLGGRGITLMG 107
Q + L IG + + LPPS ++ N ++ A VS LG +TL G
Sbjct: 174 QRSAIVDGGLHIGALQSLQPEDLPPSRVVLRDTNVTAVPASGAPAAVSVLGASELTLDG 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1570HTHTETR280.002 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 28.4 bits (63), Expect = 0.002
Identities = 8/37 (21%), Positives = 17/37 (45%), Gaps = 5/37 (13%)

Query: 4 LSWIIFGLIAGILAKWIMPG-----KDGGGFFMTIIL 35
+ I+ G I+G++ W+ K ++ I+L
Sbjct: 163 AAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILL 199


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1599ENTEROVIROMP1944e-66 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 194 bits (494), Expect = 4e-66
Identities = 66/187 (35%), Positives = 93/187 (49%), Gaps = 18/187 (9%)

Query: 1 MKNIILSTLVITTSVLVVNVAQADTNAFSVGYAQSKVQDFKN-IRGVNVKYRYE-DDSPV 58
MK I + + + A T+ + GYAQS Q N + G N+KYRYE D+SP+
Sbjct: 1 MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKMGGFNLKYRYEEDNSPL 60

Query: 59 SFISSLSYLYGDSQASGSIESEGIHYHDKFEVKYGSLMVGPAYRLSDNFSLYALAGVGTV 118
I S +Y AS D + +Y + GPAYR++D S+Y + GVG
Sbjct: 61 GVIGSFTYTEKSRTASSG---------DYNKNQYYGITAGPAYRINDWASIYGVVGVGYG 111

Query: 119 KATFKEHATQDGDSFSNKISSRKTGFAWGAGVQMNPLENIVVDVGYEGSNISSTKINGFN 178
K E+ T D+ GF++GAG+Q NP+EN+ +D YE S I S + +
Sbjct: 112 KFQTTEYPTYKHDT-------SDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWI 164

Query: 179 VGVGYRF 185
GVGYRF
Sbjct: 165 AGVGYRF 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1606cdtoxinb298e-104 Cytolethal distending toxin B signature.
		>cdtoxinb#Cytolethal distending toxin B signature.

Length = 269

Score = 298 bits (764), Expect = e-104
Identities = 126/276 (45%), Positives = 167/276 (60%), Gaps = 16/276 (5%)

Query: 1 MKKPVFFLLTMIICSYISFACANISDYKVMTWNLQGSSASTESKWNVNVRQLLSGTAGVD 60
MKK + L+ + S+ + A +++D++V TWNLQG+SA+TESKWN+NVRQL+SG VD
Sbjct: 1 MKKYIISLI--VFLSFYAQA--DLTDFRVATWNLQGASATTESKWNINVRQLISGENAVD 56

Query: 61 ILMVQEAGAVPTSAVPTGRHIQPFGVGIPIDEYTWNLGTTSRQDIRYIYYSAIDVGARRV 120
IL VQEAG+ P++AV TG I GIP+ E WNL T SR YIY+SA+D RV
Sbjct: 57 ILAVQEAGSPPSTAVDTGTLIP--SPGIPVRELIWNLSTNSRPQQVYIYFSAVDALGGRV 114

Query: 121 NLAIVSRQRADNVYVLRPTTVASRPVIGIGLGNDVFLTAHALASGGPDAAAIVRVTINFF 180
NLA+VS +RAD V+VL P RP++GI +GND F TAHA+A DA A+V NFF
Sbjct: 115 NLALVSNRRADEVFVLSPVRQGGRPLLGIRIGNDAFFTAHAIAMRNNDAPALVEEVYNFF 174

Query: 181 RQ---PQMRHLSWFLAGDFNRSPDRLENDLMTEHLERVVAVLAPTEPTQISGGILDYGVI 237
R P + L+W + GDFNR P LE +L T + R +++P TQ S LDY V
Sbjct: 175 RDSRDPVHQALNWMILGDFNREPADLEMNL-TVPVRRASEIISPAAATQTSQRTLDYAVA 233

Query: 238 VDRAPYSQR------VEALRNPQLASDHYPVAFLAR 267
+ + V R Q++SDH+PV R
Sbjct: 234 GNSVAFRPSPLQAGIVYGARRTQISSDHFPVGVSRR 269


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1609BORPETOXINA812e-20 Bordetella pertussis toxin A subunit signature.
		>BORPETOXINA#Bordetella pertussis toxin A subunit signature.

Length = 269

Score = 81.4 bits (200), Expect = 2e-20
Identities = 57/170 (33%), Positives = 85/170 (50%), Gaps = 12/170 (7%)

Query: 22 VYRVDSTPPDVIFRDGFSLLGYNRNFQQFISGRSCSGGSSDSRYIATTSSVNQT------ 75
VYR DS PP+ +F++GF+ G N N ++GRSC GSS+S +++T+SS T
Sbjct: 41 VYRYDSRPPEDVFQNGFTAWGNNDNVLDHLTGRSCQVGSSNSAFVSTSSSRRYTEVYLEH 100

Query: 76 ---YAIARAYYSRSTFKGNLYRYQIRADNNFYSLLPS-ITYLETQGGHFN-AYEKTMMRL 130
A+ R T Y Y++RADNNFY S Y++T G + +
Sbjct: 101 RMQEAVEAERAGRGTGHFIGYIYEVRADNNFYGAASSYFEYVDTYGDNAGRILAGALATY 160

Query: 131 QREYVSTLSILPENIQKAVALVYDSATGLVKDGVSTMNASYLGLSTTSNP 180
Q EY++ I PENI++ + ++ TG NA Y+ T +NP
Sbjct: 161 QSEYLAHRRIPPENIRRVTRVYHNGITGETTT-TEYSNARYVSQQTRANP 209


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1610BORPETOXINB354e-05 Bordetella pertussis toxin B subunit signature.
		>BORPETOXINB#Bordetella pertussis toxin B subunit signature.

Length = 226

Score = 35.0 bits (80), Expect = 4e-05
Identities = 31/101 (30%), Positives = 42/101 (41%), Gaps = 7/101 (6%)

Query: 30 TNAYYSDEVISELHVGQIDTSPYFCIKTVKANGSGTPVV-ACAVSKQSIWAPSFKELLDQ 88
T+ YYS+ + L T+ C V+ SG PV+ AC + + L
Sbjct: 126 TDHYYSNVTATRLLS---STNSRLCAVFVR---SGQPVIGACTSPYDGKYWSMYSRLRKM 179

Query: 89 ARYFYSTGQSVRIHVQKNIWTYPLFVNTFSANALVGLSSCS 129
Y G SVR+HV K Y TF AL G+S C+
Sbjct: 180 LYLIYVAGISVRVHVSKEEQYYDYEDATFETYALTGISICN 220


28SPA1660SPA1673Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA16604172.55604450S ribosomal protein L32
SPA16613162.306362hypothetical protein
SPA16633151.694008hypothetical protein
SPA16642141.838034ribosomal large subunit pseudouridine synthase
SPA16651142.303953hypothetical protein
SPA16661152.473201ribonuclease E
SPA1667-1141.395952flagellar hook-associated protein 3
SPA1668-1142.344374flagellar hook-associated protein 1
SPA1669-1163.502418flagellar protein FlgJ
SPA16701143.279424flagellar P-ring protein
SPA16712152.895768flagellar L-ring protein
SPA16722152.840717flagellar basal-body rod protein FlgG (distal
SPA16732123.012563flagellar basal-body rod protein FlgF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1666IGASERPTASE569e-10 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 55.8 bits (134), Expect = 9e-10
Identities = 50/259 (19%), Positives = 93/259 (35%), Gaps = 26/259 (10%)

Query: 513 PSEEEYAERKRPEQPALATFAMPDVPPAPTPVEPAVSVATAKKDNVAAEQPAQPGLFSRF 572
P E+ + DVP P+ + A+ D A P P S
Sbjct: 983 PEVEKRNQTVDTTNITTPNNIQADVPSVPSN-----NEEIARVDE-APVPPPAPATPSET 1036

Query: 573 LNALKQLFSGEETKTVETAAPKAEEKAERQQDRRKPRQNNRRDRNERRDTRDNRAGRDGG 632
S +E+KTVE A E + ++ K ++N + +T+ N + G
Sbjct: 1037 TE-TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKA-----NTQTNEVAQSGS 1090

Query: 633 ESRDDNRRNRRQAQQQNAEAR---DTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQA 689
E+++ ++ E + +T + + KV + Q +P++E+S A
Sbjct: 1091 ETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS----QVSPKQEQSETVQPQAEPA 1146

Query: 690 QQEVKALNREEQPVQETEQEERVQQVQPRRKQRQLNQKVRFTNSTVVETVDTPVVVDEPR 749
++ +N +E Q + QP ++ N + T ST V T ++ V E
Sbjct: 1147 RENDPTVNIKEPQSQTNTTAD---TEQPAKETSS-NVEQPVTESTTVNTGNSVVENPENT 1202

Query: 750 PVENVEQPVPAPRTELAKV 768
+ P +E +
Sbjct: 1203 TPATTQ---PTVNSESSNK 1218



Score = 38.5 bits (89), Expect = 2e-04
Identities = 51/372 (13%), Positives = 88/372 (23%), Gaps = 47/372 (12%)

Query: 630 DGGESRDDNRRNRRQAQQQNAEARDTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQA 689
D G + R + N E Q + T + Q S +
Sbjct: 963 DLGAWKYKLRNVNGRYDLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDE 1022

Query: 690 QQEVKALNREEQPVQETEQEERVQQVQPRRKQRQLNQKVRFTNSTVVETVDTPVVVDEPR 749
ET E Q+ + K Q + N V + V +
Sbjct: 1023 APVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKE-AKSNVKANTQ 1081

Query: 750 PVENVEQPVPAPRTELAKVDLPVVADIAPEQDDSVEPRDNTGMPRRSRRSPRHLRVSGQR 809
E + T+ + A + E+ VE +++ P+ +
Sbjct: 1082 TNEVAQSGSETKETQTTETKET--ATVEKEEKAKVETE-------KTQEVPKVTSQVSPK 1132

Query: 810 RRRYRDERYPTQSPMPLTVACASPEMASGKVWIRYPIVRPQETQVVEEQREADLALPQPV 869
+ + + + P V +E Q + AD P
Sbjct: 1133 QEQSETVQPQAEPARE-----------------NDPTVNIKEPQS-QTNTTADTEQPAKE 1174

Query: 870 VAEPQVIAATVALEPQASVQAVENVAVEPQTVAEPQAPEVVKVETTHPEVIAAPVDEQPQ 929
Q V + + PE TT P V + ++
Sbjct: 1175 T-------------SSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKN 1221

Query: 930 LIAESDTPEAQEVIA------DAEPVAETADASITVAENVADVVVVEPEEETKAEAAVVE 983
S V D VA S ++D AV +
Sbjct: 1222 RHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQ 1281

Query: 984 HTAEETVIAPAQ 995
H ++ + Q
Sbjct: 1282 HISQLEMNNEGQ 1293


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1667FLAGELLIN414e-06 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 41.2 bits (96), Expect = 4e-06
Identities = 30/138 (21%), Positives = 59/138 (42%)

Query: 1 MRISTQMMYEQNMSGITNSQAEWMKLGEQMSTGKRVTNPSDDPIAASQAVVLSQAQAQNS 60
I+T + + + SQ+ E++S+G R+ + DD + A + +
Sbjct: 2 QVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLT 61

Query: 61 QYALARTFATQKVSLEESVLSQVTTAIQTAQEKIVYAGNGTLSDDDRASLATDLQGIRDQ 120
Q + E L+++ +Q +E V A NGT SD D S+ ++Q ++
Sbjct: 62 QASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEE 121

Query: 121 LMNLANSTDGNGRYIFAG 138
+ ++N T NG + +
Sbjct: 122 IDRVSNQTQFNGVKVLSQ 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1668FLGHOOKAP16640.0 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 664 bits (1714), Expect = 0.0
Identities = 438/553 (79%), Positives = 487/553 (88%), Gaps = 8/553 (1%)

Query: 2 SSLINHAMSGLNAAQAALNTVSNNINNYNVAGYTRQTTILAQANSTLGAGGWIGNGVYVS 61
SSLIN+AMSGLNAAQAALNT SNNI++YNVAGYTRQTTI+AQANSTLGAGGW+GNGVYVS
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVS 60

Query: 62 GVQREYDAFITNQLRGAQNQSSGLTTRYEQMSKIDNLLADKSSSLSGSLQSFFTSLQTLV 121
GVQREYDAFITNQLR AQ QSSGLT RYEQMSKIDN+L+ +SSL+ +Q FFTSLQTLV
Sbjct: 61 GVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLV 120

Query: 122 SNAEDPAARQALIGKAEGLVNQFKTTDQYLRDQDKQVNIAIGSSVAQINNYAKQIANLND 181
SNAEDPAARQALIGK+EGLVNQFKTTDQYLRDQDKQVNIAIG+SV QINNYAKQIA+LND
Sbjct: 121 SNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLND 180

Query: 182 QISRMTGVGAGASPNDLLDQRDQLVSELNKIVGVEVSVQDGGTYNLTMANGYTLVQGSTA 241
QISR+TGVGAGASPN+LLDQRDQLVSELN+IVGVEVSVQDGGTYN+TMANGY+LVQGSTA
Sbjct: 181 QISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTA 240

Query: 242 RQLAAVPSSADPTRTTVAYVDEAAGNIEIPEKLLNTGSLGGLLTFRSQDLDQTRNTLGQL 301
RQLAAVPSSADP+RTTVAYVD AGNIEIPEKLLNTGSLGG+LTFRSQDLDQTRNTLGQL
Sbjct: 241 RQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQL 300

Query: 302 ALAFADAFNAQHTKGYDADGNKGKDFFSIGSPVVYSNSNNADKTVSLTAKVVDSTKVQAT 361
ALAFA+AFN QH G+DA+G+ G+DFF+IG P V N+ N V++ A V D++ V AT
Sbjct: 301 ALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGD-VAIGATVTDASAVLAT 359

Query: 362 DYKIVFDGTDWQVTRTADNTTFTATKDADGKLEIDGLKVTVGTGAQKNDSFLLKPVSNAI 421
DYKI FD WQVTR A NTTFT T DA+GK+ DGL++T NDSF LKPVS+AI
Sbjct: 360 DYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAI 419

Query: 422 VDMNVKVTNEAEIAMASESKLDPDVDTGDSDNRNGQALLDLQ-NSNVVGGNKTFNDAYAT 480
V+M+V +T+EA+IAMASE D GDSDNRNGQALLDLQ NS VGG K+FNDAYA+
Sbjct: 420 VNMDVLITDEAKIAMASEE------DAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYAS 473

Query: 481 LVSDVGNKTSTLKTSSTTQANVVKQLYKQQQSVSGVNLDEEYGNLQRYQQYYLANAQVLQ 540
LVSD+GNKT+TLKTSS TQ NVV QL QQQS+SGVNLDEEYGNLQR+QQYYLANAQVLQ
Sbjct: 474 LVSDIGNKTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQ 533

Query: 541 TANALFDALLNIR 553
TANA+FDAL+NIR
Sbjct: 534 TANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1669FLGFLGJ4990.0 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 499 bits (1285), Expect = 0.0
Identities = 263/316 (83%), Positives = 289/316 (91%), Gaps = 3/316 (0%)

Query: 1 MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDG 60
MI D KLLASAAWDAQSLNELKAKAG+DPAANIRPVARQVEGMFVQMMLKSMR+ALPKDG
Sbjct: 1 MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG 60

Query: 61 LFSSDQTRLYTSMYDQQIAQQMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLET 120
LFSS+ TRLYTSMYDQQIAQQMTAGKGLGLA+MMVKQMT Q +P + P P+KF LET
Sbjct: 61 LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET 120

Query: 121 VNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLSLPARLASEQSGVPHHLILAQ 180
V YQNQAL+QLV+KA+P+ D S L GDSK FLA+LSLPA+LAS+QSGVPHHLILAQ
Sbjct: 121 VVRYQNQALSQLVQKAVPRNYDDS---LPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQ 177

Query: 181 AALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS 240
AALESGWGQRQI RENGEPSYN+FGVKA+ +WKGPVTEITTTEYENGEAKKVKAKFRVYS
Sbjct: 178 AALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYS 237

Query: 241 SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAM 300
SYLEALSDYV LLTRNPRYAAVTTAA+AEQGA ALQ+AGYATDP+YARKLT+MIQQ+K++
Sbjct: 238 SYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKSI 297

Query: 301 SEKVSKTYSANLDNLF 316
S+KVSKTYS N+DNLF
Sbjct: 298 SDKVSKTYSMNIDNLF 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1670FLGPRINGFLGI429e-153 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 429 bits (1104), Expect = e-153
Identities = 153/362 (42%), Positives = 215/362 (59%), Gaps = 9/362 (2%)

Query: 5 LAGIVLALVATLAHAERIRDLTSVQGVRENSLIGYGLVVGLDGTGDQTTQTPFTTQTLNN 64
A L+ A RI+D+ S+Q R+N LIGYGLVVGL GTGD +PFT Q++
Sbjct: 14 SALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRA 73

Query: 65 MLSQLGITVPTGTNMQLKNVAAVMVTASYPPFARQGQTIDVVVSSMGNAKSLRGGTLLMT 124
ML LGIT G + KN+AAVMVTA+ PPFA G +DV VSS+G+A SLRGG L+MT
Sbjct: 74 MLQNLGITTQGGQS-NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMT 132

Query: 125 PLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQLNGGRITNGAIIERELPTQFGAGNT 184
L G D Q+YA+AQG ++V G A +++ R+ NGAIIERELP++F
Sbjct: 133 SLSGADGQIYAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVN 192

Query: 185 INLQLNDEDFTMAQQITDAINRAR----GYGSATALDARTVQVRVPSGNSSQVRFLADIQ 240
+ LQL + DF+ A ++ D +N G A D++ + V+ P + R +A+I+
Sbjct: 193 LVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPRV-ADLTRLMAEIE 251

Query: 241 NMEVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQLNVNQPNTPFGGG 300
N+ V T AKVVIN RTG++V+ +V + AV+ G L+V V V QP PF G
Sbjct: 252 NLTVE-TDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQP-APFSRG 309

Query: 301 QTVVTPQTQIDLRQSGGSLQSVRSSANLNSVVRALNALGATPMDLMSILQSMQSAGCLRA 360
QT V PQT I Q G + ++ +L ++V LN++G +++ILQ ++SAG L+A
Sbjct: 310 QTAVQPQTDIMAMQEGSKV-AIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQA 368

Query: 361 KL 362
+L
Sbjct: 369 EL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1671FLGLRINGFLGH353e-127 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 353 bits (908), Expect = e-127
Identities = 211/232 (90%), Positives = 223/232 (96%)

Query: 1 MQKYALHAYPVMALMVATLTGCAWIPAKPLVQGATTAQPIPGPVPVANGSIFQSAQPINY 60
MQK A H Y + +L+V +LTGCAWIP+ PLVQGAT+AQP+PGP PVANGSIFQSAQPINY
Sbjct: 1 MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY 60

Query: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTSFGFDTVPRYLQGLFGNS 120
GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT+FGFDTVPRYLQGLFGN+
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA 120

Query: 121 RADMEASGGNSFNGKGGANASNTFSGTLTVTVDQVLANGNLHVVGEKQIAINQGTEFIRF 180
RAD+EASGGN+FNGKGGANASNTFSGTLTVTVDQVL NGNLHVVGEKQIAINQGTEFIRF
Sbjct: 121 RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF 180

Query: 181 SGVVNPRTISGSNSVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232
SGVVNPRTISGSN+VPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM
Sbjct: 181 SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1672FLGHOOKAP1444e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 43.8 bits (103), Expect = 4e-07
Identities = 18/81 (22%), Positives = 36/81 (44%), Gaps = 14/81 (17%)

Query: 3 SSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDLLYQTIRQPGAQSSEQTTL 62
S + A +GL+A Q ++ +NN+++ + G+ RQ + + +TL
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI--------------MAQANSTL 47

Query: 63 PSGLQIGTGVRPVATERLHSQ 83
+G +G GV +R +
Sbjct: 48 GAGGWVGNGVYVSGVQREYDA 68



Score = 41.1 bits (96), Expect = 3e-06
Identities = 11/41 (26%), Positives = 21/41 (51%)

Query: 220 ETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL 260
S VN+ EE N+ + Q+ Y N++ + T + + L +
Sbjct: 505 SISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


29SPA1704SPA1727Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1704031-6.288676hypothetical protein
SPA1705032-8.406579hypothetical protein
SPA1706-128-7.625288hypothetical protein
SPA1707024-6.431059major curlin subunit precursor
SPA1708-124-5.977132curlin monomer nucleation protein
SPA1709-219-4.590537regulatory protein
SPA1710015-1.697053assembly/transport component in curli
SPA1712-116-2.160595assembly/transport component in curli
SPA1713119-3.304192hypothetical protein
SPA1714023-5.434030hypothetical protein
SPA1715126-5.441201hypothetical protein
SPA1716228-6.5666642-hydroxyacid dehydrogenase
SPA1718231-7.516915*oxidoreductase
SPA1719130-7.038813transporter
SPA1720121-4.956386hypothetical protein
SPA1722-390.254237hypothetical protein
SPA1723-390.526788sodium/glucose cotransporter
SPA1724-2102.144568transcriptional regulator
SPA17250112.799552PhoH protein (phosphate starvation-inducible
SPA1726-1103.513221sodium/proline symporter (proline permease)
SPA17270123.973537proline dehydrogenase (proline oxidase)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1719TCRTETA523e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 51.7 bits (124), Expect = 3e-09
Identities = 52/253 (20%), Positives = 91/253 (35%), Gaps = 24/253 (9%)

Query: 56 AFLATAAFIGRPFGGALFGLLADKFGRKPLMMWSIVAYSVGTGLSGLASGVIMLTLSRFI 115
A A F P GAL +D+FGR+P+++ S+ +V + A + +L + R +
Sbjct: 50 ALYALMQFACAPVLGAL----SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105

Query: 116 VGMGMAGEYACASTYAVESWPKHLKSKASAFLVSGFGIGNIIAAYFMPSFAEAYGWRAAF 175
G+ A + A + +++ F+ + FG G ++A + + A F
Sbjct: 106 AGITGATGAVAGAYIA-DITDGDERARHFGFMSACFGFG-MVAGPVLGGLMGGFSPHAPF 163

Query: 176 FV-GLLPVLLVIYIRARAPESKEWEE--AKLSGPGKHSQSAWSVFSLSMKGLFNQA---- 228
F L L + PES + E + + W+ + L
Sbjct: 164 FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQ 223

Query: 229 ---QFPLTLCVFIVLFSIFGANWPIFGLLPTYLAGEGFDTGVVSNLMTAAAFGTVLGN-- 283
Q P L V+F +W + LA G ++ M LG
Sbjct: 224 LVGQVPAAL---WVIFGEDRFHWDA-TTIGISLAAFGI-LHSLAQAMITGPVAARLGERR 278

Query: 284 -IVWGLCADRIGL 295
++ G+ AD G
Sbjct: 279 ALMLGMIADGTGY 291


30SPA1743SPA1762Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA17430233.5386444-hydroxyphenylacetate permease
SPA17440244.2267242,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
SPA1745-1242.7388082-oxo-hepta-3-ene-1,7-dioic acid hydratase
SPA1746-2212.1191945-carboxymethyl-2-hydroxymuconate
SPA1747-2191.8058023,4-dihydroxyphenylacetate 2,3-dioxygenase
SPA1748-1141.4394414-hydroxyphenylacetate catabolism
SPA1750-215-1.063251homoprotocatechuate degradative operon
SPA1751-216-1.2777104-hydroxyphenylacetate 3-monooxygenase
SPA1752-225-2.3169574-hydroxyphenylacetate 3-monooxygenase coupling
SPA1753126-4.548908hypothetical protein
SPA1754125-4.992403response regulator
SPA1755328-6.640745histidine kinase
SPA1757338-10.277209hypothetical protein
SPA1758338-10.119887hypothetical protein
SPA1759333-8.815818cell invasion protein
SPA1760332-9.156860cell invasion protein
SPA1761329-8.092626inner membrane protein
SPA1762223-5.232791hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1754HTHFIS831e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 83.0 bits (205), Expect = 1e-20
Identities = 30/117 (25%), Positives = 56/117 (47%), Gaps = 1/117 (0%)

Query: 2 KILLIEDNQKTIEWVRQGLTEAGYMVDYACDGRDGLHLALQEHYSLIILDIMLPGLDGWQ 61
IL+ +D+ + Q L+ AGY V + L++ D+++P + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLRALRTAHQS-PVICLTARDSVEDRVKGLEAGANDYLVKPFSFAELLARVRAQLRQ 117
+L ++ A PV+ ++A+++ +K E GA DYL KPF EL+ + L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1755PF06580340.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 33.7 bits (77), Expect = 0.001
Identities = 18/102 (17%), Positives = 38/102 (37%), Gaps = 15/102 (14%)

Query: 348 ILLQRVLSNLLTNAIRYSDENAVIRIESAYDDNVAEIRVANPGSHPADADKLFRRFWRGD 407
+L+Q ++ N + + I + I ++ D+ + V N GS K
Sbjct: 258 MLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE-------- 309

Query: 408 NARHTAGFGLGLSLVNA-IALLHGGSASYRYADEHNIFSVRL 448
G GL V + +L+G A + +++ + +
Sbjct: 310 ------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMV 345


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1759TYPE3OMBPROT6550.0 Type III secretion system outer membrane B protein ...
		>TYPE3OMBPROT#Type III secretion system outer membrane B protein

family signature.
Length = 538

Score = 655 bits (1692), Expect = 0.0
Identities = 186/396 (46%), Positives = 253/396 (63%), Gaps = 5/396 (1%)

Query: 166 LNNQPWQTIKNTLTHNGHHYTNTQLPAAEMKIGAKDIFPSAYQGKGVCSWDTKNIHHANN 225
LNN+ W + ++H+G +Y PA+ MKIG K+IF Y GKG+C T+ H N
Sbjct: 146 LNNKNWGPVNKNISHHGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIAN 205

Query: 226 LWMSTVSVHEDGKDKTLFCGIRHGVLSPYH-EKDPLLRQVGAENKAKEVLTAALYSKPEL 284
+W+S V V ++GK+ +F GIRHGV+S Y +K+ R V A NKA+E+++AALYS+PEL
Sbjct: 206 MWLSKV-VDDEGKE--IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPEL 262

Query: 285 LNRALEGEAVSLKLVSVGLLTASNIFGKEGTMVEDQMRAWQSL-TQPGKMIHLKIRNKDG 343
L++AL G+ V LK+VS LLT +++ G E +M++DQ+ A + L ++ G+ L IRN DG
Sbjct: 263 LSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDG 322

Query: 344 DLQTVKIKPDVAAFNVGVNELALKLGFGLKASDRYNAEALHQLLGNDLRPEARPGGWVGE 403
L+ V + V FN GVNELALK+G G + D+ N E++ LLG++ GGW E
Sbjct: 323 LLKEVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAE 382

Query: 404 WLAQYPDNYEVVNTLARQIKDIWKNNQHHKDGGEPYKLAQRLAMLAHEIDAVPAWNCKSG 463
+ + P V LA QIK+I D GEPYKL+QR+ +LA+ I AVP WNCKSG
Sbjct: 383 AIEKNPPCKNDVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSG 442

Query: 464 KDRTGMMDSEIKREHISLHQTHMLSAPGSLPDSGGQKIFQKVLLNSGNLEIQKQNTGGAG 523
KDRTGM D+EIKRE I H+T S S S +++F +L+NSGN+EIQ+ NTG G
Sbjct: 443 KDRTGMQDAEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPG 502

Query: 524 NKVMKNLSPEVLNLSYQKRVGDENIWQSVKGISSLI 559
NKVMK L L LSY +R+GD IW VKG SS +
Sbjct: 503 NKVMKKLPLSSLELSYSERIGDSKIWNMVKGYSSFV 538


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1760PF078241651e-56 Type III secretion chaperone
		>PF07824#Type III secretion chaperone

Length = 120

Score = 165 bits (419), Expect = 1e-56
Identities = 33/114 (28%), Positives = 63/114 (55%), Gaps = 1/114 (0%)

Query: 1 MESLLNRLYDALGLDAPE-DEPLLIIDDGIQVYFNESDHTLEMCCPFMPLPDDILTLQHF 59
ME L + + ALG+ + + D+ +++DD + +Y + ++ + CPF LP++I L +
Sbjct: 1 MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLLCPFCALPENINDLIYA 60

Query: 60 LRLNYTSAVTIGADADNTALVALYRLPQTSTEEEALTGFELFISNVKQLKEHYA 113
L LNY+ + + D + +L+A L + E+ E +IS V+ LK+ +A
Sbjct: 61 LSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYYISRVRWLKDEFA 114


31SPA1822SPA1838Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1822224-2.544015hypothetical protein
SPA1823122-3.127536hypothetical protein
SPA1824322-3.530669formate transporter
SPA1825222-3.337824formate acetyltransferase 1
SPA1827415-2.737197hypothetical protein
SPA1828415-1.546683pyruvate formate-lyase 1 activating enzyme
SPA1829211-0.488379transport protein
SPA1830-1130.879816transport protein
SPA1831-2141.922086hypothetical protein
SPA1832-3131.660652dimethyl sulfoxide reductase subunit C
SPA1833-2122.769790anaerobic dimethyl sulfoxide reductase subunit
SPA1834-1112.371411anaerobic dimethyl sulfoxide reductase subunit
SPA1835-1112.855591seryl-tRNA synthetase
SPA1836-2103.293342hypothetical protein
SPA1837-2113.333403outer membrane lipoprotein carrier protein
SPA1838-2113.706676cell division protein, required for cell
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1830TCRTETB320.004 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 32.2 bits (73), Expect = 0.004
Identities = 36/158 (22%), Positives = 63/158 (39%), Gaps = 6/158 (3%)

Query: 8 VMLLLCGLLLLTLAIAVLNTLVLLWLAQA-NLPTWQVGMVSSSYFTGNLVGTLFTGYLIK 66
+++ LC L ++ ++ + L +A N P V++++ +GT G L
Sbjct: 15 ILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD 74

Query: 67 RIGFNRSYYLASLIFAAGCVGLGVMVGFWSWMSW-RFIAGIGCAMIWVVVESALMCSGTS 125
++G R +I G V V F+S + RFI G G A +V +
Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK 134

Query: 126 HNRGRLLAAYMMVYYMGTFLGQLLVSKVSGELLHVLPW 163
NRG+ + MG +G + G + H + W
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPA----IGGMIAHYIHW 168


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1832BCTERIALGSPC280.035 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 28.0 bits (62), Expect = 0.035
Identities = 12/51 (23%), Positives = 20/51 (39%), Gaps = 4/51 (7%)

Query: 97 LKKLPP-ALRTLWLIITMVLGVVFVW---MMVRVYNSIDTVPTWYSVWTPL 143
+ KLPP + + I+ +L ++F M+ D P TP
Sbjct: 3 ISKLPPLSPSVIRRILFYLLMLLFCQQLAMIFWRIGLPDNAPVSSVQITPA 53


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1838IGASERPTASE583e-10 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 58.2 bits (140), Expect = 3e-10
Identities = 47/292 (16%), Positives = 85/292 (29%), Gaps = 44/292 (15%)

Query: 555 AAPAFSLATGGAPRPQVKEGIGPQLPRPNRVRVPTRRELASYGIKLPSQRIAEEKAREAE 614
+ A P V ++ R + VP PS+ E A ++
Sbjct: 994 TTNITTPNNIQADVPSVPSN-NEEIARVDEAPVPPPAP------ATPSET-TETVAENSK 1045

Query: 615 RNQYETGVQLTDEEIDAMHQDELARQFAQSQQHRYGETYQHDTQQAEDDDTAAEAELARQ 674
+ E DA R+ A+ + + +TQ E + +E + +
Sbjct: 1046 QESKTVEKN----EQDATETTAQNREVAKEAK----SNVKANTQTNEVAQSGSETKETQT 1097

Query: 675 FAASQQQRYSGEQPAGAQPFSLDDLDFSPMKVLVDEGPHEPLFTPGVMPESTPVQQPVAP 734
+ E+ A KV ++ P T V P+ +Q
Sbjct: 1098 TETKETATVEKEEKA---------------KVETEKTQEVPKVTSQVSPKQ---EQSETV 1139

Query: 735 QPQPQYQQSQQPVAPQSQYQQPQQPVAPQPQPQYQQSQQPVAPQSQYQQPQQPVAPQPQY 794
QPQ + + P + Q A QP + S P ++ V
Sbjct: 1140 QPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTE----STTVNTGNSV 1195

Query: 795 QQPQQPVAPQPQYQQPQQPTA----PQPQYQQPVAPQPQYQQPQQPVAPQPQ 842
+ P P QP + P+ ++++ V P +P +
Sbjct: 1196 --VENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS 1245



Score = 43.5 bits (102), Expect = 7e-06
Identities = 26/214 (12%), Positives = 55/214 (25%), Gaps = 37/214 (17%)

Query: 718 TPGVMPESTPVQQPVAPQ-------PQPQYQQSQQPVAPQSQYQQPQQPVAPQPQPQYQQ 770
T P + P P P P + + + + +
Sbjct: 995 TNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKN 1054

Query: 771 SQQPVAPQSQYQ-----------------------------QPQQPVAPQPQYQQPQQPV 801
Q +Q + Q + ++ + V
Sbjct: 1055 EQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKV 1114

Query: 802 APQPQYQQPQQPTAPQPQYQQPVAPQPQYQQPQQPVAPQPQYQQPQQPTAPQDSLIHPLL 861
+ + P+ + P+ +Q QPQ +P + P ++PQ T P
Sbjct: 1115 ETEKTQEVPKVTSQVSPKQEQSETVQPQ-AEPARENDPTVNIKEPQSQTNTTADTEQPAK 1173

Query: 862 MRNGDSRPLQRPTTPLPSLDLLTPPPSEVEPVDT 895
+ + +T + + + + P P T
Sbjct: 1174 ETSSNVEQPVTESTTVNTGNSVVENPENTTPATT 1207



Score = 41.2 bits (96), Expect = 3e-05
Identities = 46/303 (15%), Positives = 85/303 (28%), Gaps = 46/303 (15%)

Query: 296 RATQPEYDEYDPLLNGHSVTEPVAAAAAATAVTQTWAASADP--IMQTPPMPGAEPVVAQ 353
PE ++ + ++ ++T P A +V A PP P +
Sbjct: 979 DLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTE 1038

Query: 354 PTVEWQP--------------VPGPQTGE------PVIAPAPEGYQPHPQYAQPQEAQSA 393
E Q E + + + ++ +E Q+
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTT 1098

Query: 394 PWQQPVPVASAPQYAATPATAAEYDS----LAPQETQPQWQAPDAEQHWQPEPT------ 443
++ V + E ++P++ Q + P AE + +PT
Sbjct: 1099 ETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEP 1158

Query: 444 ---HQPEPIAAEPSHMPPPVIEQPVTT---------EPEPGIEETRPARPPLYYFEEVEE 491
+P+ +EQPVT E T P E +
Sbjct: 1159 QSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNK 1218

Query: 492 KRAREREQLAAWYQPIPEPVKENVPVKPTVSVAPSIPPVEAVAAAASLDAGIKSGALAAG 551
+ R R + + EP + + TV++ A + A + AL G
Sbjct: 1219 PKNRHRRSVRS-VPHNVEPATTSSNDRSTVALCDLT-STNTNAVLSDARAKAQFVALNVG 1276

Query: 552 AAA 554
A
Sbjct: 1277 KAV 1279



Score = 40.0 bits (93), Expect = 7e-05
Identities = 21/187 (11%), Positives = 56/187 (29%), Gaps = 12/187 (6%)

Query: 724 ESTPVQQPVAPQPQPQYQQSQQPVAPQSQYQQPQQPVAPQPQPQYQ-QSQQPVAPQSQYQ 782
++ + A + Q ++ + + VA + Q+ + + +
Sbjct: 1049 KTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEK 1108

Query: 783 QPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPTAPQPQYQQPVAPQPQYQQPQQPVAPQPQ 842
+ + V + + P+ P+ +Q + PQ + + P ++PQ
Sbjct: 1109 EEKAKVETEKTQEVPKVTSQVSPKQEQSET-VQPQAEPARENDPTVNIKEPQSQTNTTAD 1167

Query: 843 YQQPQQPTAPQDSLIHPLLMRNGDSRPLQRPT-TPLPSLDLLTPPPSEVEPVDTFALEQM 901
+QP + T+ + + + P + P+ +P
Sbjct: 1168 TEQPAKETSSN---VEQPVTESTTVNTGNSVVENPENTT------PATTQPTVNSESSNK 1218

Query: 902 ARLVEAR 908
+ R
Sbjct: 1219 PKNRHRR 1225



Score = 38.9 bits (90), Expect = 2e-04
Identities = 31/215 (14%), Positives = 65/215 (30%), Gaps = 25/215 (11%)

Query: 658 QQAEDDDTAAEAELARQFAASQQQRYSGEQPAGAQPFSLDDLDFSPMKVLVDEGPHEPLF 717
AE+ ++ + A++ + E A+ ++ + V + E
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKS----NVKANTQTNEVAQSGSE--- 1091

Query: 718 TPGVMPESTPVQQPVAPQPQPQYQQSQQPVAPQSQYQ-QPQQPVAPQPQPQYQQSQQPVA 776
T T V + + + + + P+ Q P+Q + QPQ + +++
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREN-D 1150

Query: 777 PQSQYQQPQQPVAPQPQYQQPQQPVAPQ-PQYQQPQQPTAPQPQYQQ-PVAPQPQYQQPQ 834
P ++PQ +QP + + Q + P P QP
Sbjct: 1151 PTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPT 1210

Query: 835 --------------QPVAPQPQYQQPQQPTAPQDS 855
+ V P +P ++ S
Sbjct: 1211 VNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS 1245



Score = 36.6 bits (84), Expect = 0.001
Identities = 60/345 (17%), Positives = 98/345 (28%), Gaps = 37/345 (10%)

Query: 365 QTGEPVIAPAPEGYQP-HPQYAQPQEAQSAPWQQPVPVASAPQYAATPATAAEYDSLAPQ 423
QT + P Q P E + + PVP P ATP+ E
Sbjct: 990 QTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVP----PPAPATPSETTE----TVA 1041

Query: 424 ETQPQWQAPDAEQHWQP-EPTHQPEPIAAEPSHMPPPVIEQPVTTEPEPGIEETRPARPP 482
E Q + E T Q +A E + + +ET+
Sbjct: 1042 ENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETK 1101

Query: 483 LYYFEEVEEKRAREREQLAAWYQPIPEPVKENVP-------VKPTVSVAPSIPPVEAVAA 535
E EEK E E+ Q +P+ + P V+P A P +
Sbjct: 1102 ETATVEKEEKAKVETEKT----QEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKE 1157

Query: 536 AASLDAGIKSGALAAGAAAAAPAFSLATGGAPRPQVKEGIGPQLPRPNRVRVPTRRELAS 595
S A ++ + P+ P + PT +S
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQ-PTVNSESS 1216

Query: 596 YGIKLPSQRI-------AEEKAREAERNQYETGVQLTDEEIDAMHQDELARQFAQSQQHR 648
K +R E + LT +A+ D A+ AQ
Sbjct: 1217 NKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAK--AQFVALN 1274

Query: 649 YGETYQHDTQQAEDDDTAA------EAELARQFAASQQQRYSGEQ 687
G+ Q E ++ + + +++SQ +R+S +
Sbjct: 1275 VGKAVSQHISQLEMNNEGQYNVWVSNTSMNKNYSSSQYRRFSSKS 1319



Score = 35.4 bits (81), Expect = 0.002
Identities = 17/131 (12%), Positives = 34/131 (25%), Gaps = 8/131 (6%)

Query: 723 PESTPVQQPV-APQPQ-PQYQQSQQPVAPQSQYQ--QPQQPVAPQPQPQYQQSQQPVAPQ 778
PE Q V P Q+ P P + + + + P P P +
Sbjct: 983 PEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAE 1042

Query: 779 SQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPTAPQPQYQQPVAPQPQYQQPQQP-V 837
Q+ + Q + A + + + VA + Q
Sbjct: 1043 ---NSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTE 1099

Query: 838 APQPQYQQPQQ 848
+ + ++
Sbjct: 1100 TKETATVEKEE 1110


32SPA1861SPA1878Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1861-2173.300292hypothetical protein
SPA1862-2183.716214prismane-like protein
SPA18631153.819338NADH oxidoreductase Hcr
SPA18642153.927223pyruvate dehydrogenase
SPA18653163.911503L-allo-threonine aldolase
SPA18661152.887533hypothetical protein
SPA18670141.196144oxidoreductase
SPA1868-1141.197159N-acetylmuramoyl-L-alanine amidase
SPA18690130.285485hypothetical protein
SPA1870-113-0.688474lipoprotein
SPA1871-313-1.418516arginine transport ATP-binding protein ArtP
SPA1872-212-2.600501arginine/ornithine ABC transporter
SPA1873014-3.922133arginine transport system permease protein ArtQ
SPA1874117-5.482534arginine transport system permease protein ArtM
SPA1875216-4.214468arginine-binding periplasmic protein
SPA1876118-4.017049sulfatase
SPA1877219-3.119580PTS system transporter subunit IIB
SPA1878216-1.970215transport protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1866NUCEPIMERASE552e-10 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 55.2 bits (133), Expect = 2e-10
Identities = 30/125 (24%), Positives = 49/125 (39%), Gaps = 17/125 (13%)

Query: 4 RILVLGASGYIGQHLVFALSQQGHQVRA---------AARRVERLEKQRLANVSCHKVDL 54
+ LV GA+G+IG H+ L + GHQV + + RLE HK+DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 HWPENLPTLLRD--VDTVYYLVH------GMGEGGDFIAHERQAALNVRDALRQTPVKQL 106
E + L + V+ H + + LN+ + R ++ L
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 107 IFLSS 111
++ SS
Sbjct: 122 LYASS 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1867NUCEPIMERASE662e-14 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 66.3 bits (162), Expect = 2e-14
Identities = 69/370 (18%), Positives = 123/370 (33%), Gaps = 71/370 (19%)

Query: 1 MKVLVTGATSGLGRNAVEFLRNKGISVRA---------TGRNEAMGKLLEKMGAEFVHAD 51
MK LVTGA +G + + L G V +A +LL + G +F D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 52 LTELVSSQAKVMLAGIDTLWHCS-------SFTSPWGTQQAFDLANVRATRRLGEWAVAW 104
L + + ++ S +P A+ +N+ + E
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPH----AYADSNLTGFLNILEGCRHN 116

Query: 105 GVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAAGEEVINLLAQANPQTH-- 162
+++ ++ SS S+Y + D + +A +K A E L+A +
Sbjct: 117 KIQHLLYASSSSVYGL-NRKMPFSTDDSVDHPVSLYAATKKANE----LMAHTYSHLYGL 171

Query: 163 -FTVLRPQSLFGPHDK--VFIPRLAHMMHHYGSVLLPHGGSALVDMTYYENAIHAMWLAS 219
T LR +++GP + + + + M S+ + + G D TY ++ A+
Sbjct: 172 PATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQ 231

Query: 220 QPGCDHLPS--------------GRAYNITNGENRTLRSIVQKLIDELTIDCRIRSVPYP 265
R YNI N L +Q L D L I+ + +P
Sbjct: 232 DVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQ 291

Query: 266 MLDMIARSMERFGKKSAKEPPLTHYGVSKLNFDFTLDTTRAQDELGYQPIVTLDEGIERT 325
D+ T DT + +G+ P T+ +G++
Sbjct: 292 PGDV----------------LETS-----------ADTKALYEVIGFTPETTVKDGVKNF 324

Query: 326 AAWLRDHGNL 335
W RD +
Sbjct: 325 VNWYRDFYKV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1871PF05272300.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.006
Identities = 16/50 (32%), Positives = 22/50 (44%), Gaps = 1/50 (2%)

Query: 31 LVLLGPSGAGKSSLLRVLNLLEMPRSGTLTIAGNHFDFTKTPSDKAIREL 80
+VL G G GKS+L+ L L+ S T G D + + EL
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDF-FSDTHFDIGTGKDSYEQIAGIVAYEL 647


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1872FLGFLIH300.006 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 30.1 bits (67), Expect = 0.006
Identities = 34/119 (28%), Positives = 50/119 (42%), Gaps = 31/119 (26%)

Query: 81 FDAVMAG--MDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQ 138
D+V+A M + E +QV+ TP DNSAL + QL Q
Sbjct: 112 LDSVIASRLMQMALEAARQVIGQTPTVDNSALI-------KQIQQL-----------LQQ 153

Query: 139 KFIMDKHPEITTVPYDSYQNAKLDLQNGRIDAVFGDTAVVTEW-LKANPKLVPVGDKVT 196
+ + P++ P DLQ R+D + G T + W L+ +P L P G KV+
Sbjct: 154 EPLFSGKPQLRVHPD--------DLQ--RVDDMLGATLSLHGWRLRGDPTLHPGGCKVS 202


33SPA1951SPA1965Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1951-1163.297190hypothetical protein
SPA1953-2163.369290hypothetical protein
SPA1954-2164.187145excision nuclease ABC subunit B
SPA1955-1155.169609dethiobiotin synthetase
SPA1956-1155.519481biotin synthesis protein BioC
SPA1957-1165.2082728-amino-7-oxononanoate synthase
SPA1958-1155.233498biotin synthetase
SPA19590155.650478adenosylmethionine-8-amino-7-oxononanoate
SPA1960-1145.367692hypothetical protein
SPA1961-1144.952190histidine ammonia-lyase
SPA19630143.890078histidine utilization repressor
SPA1964-1133.674219formiminoglutamase
SPA19650143.274555imidazolonepropionase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1965PRTACTNFAMLY310.013 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 30.8 bits (69), Expect = 0.013
Identities = 17/55 (30%), Positives = 24/55 (43%), Gaps = 5/55 (9%)

Query: 230 VLQTAKALGIPVKGHVEQLSLLGGAQLVSRYQGLSADHIEYLDEAGVAAMRDGGT 284
VL+ +P G +S+LG ++L L HI AGVAAM+
Sbjct: 202 VLRDTNVTAVPASGAPAAVSVLGASELT-----LDGGHITGGRAAGVAAMQGAVV 251


34SPA2006SPA2036Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA20062232.179269dihydrolipoamide succinyltransferase component
SPA20071211.5235522-oxoglutarate dehydrogenase E1 component
SPA20082220.244263succinate dehydrogenase iron-sulfur protein
SPA20091201.261697succinate dehydrogenase flavoprotein subunit
SPA2010-116-0.891077succinate dehydrogenase hydrophobic membrane
SPA2011-116-0.783560succinate dehydrogenase cytochrome b-556
SPA2012-121-6.505219hypothetical protein
SPA2013-123-8.399688citrate synthase
SPA2014339-13.377758hypothetical protein
SPA2015546-16.225136endonuclease VIII, DNA N-glycosylase with an AP
SPA2016751-18.348718hypothetical protein
SPA2017652-17.999346glycosyl transferase family protein
SPA2018448-15.623364hypothetical protein
SPA2019445-13.647199cell wall biogenesis glycosyltransferase
SPA2020340-10.665334polysaccharide export ABC transporter
SPA2021-133-7.417725galactosyltransferase
SPA2022-123-2.114508UDP-galactopyranose mutase
SPA2023-1193.317480hypothetical protein
SPA2025-2163.060038DNA recombinase
SPA2026-1153.331960hypothetical protein
SPA2027-2133.744514hypothetical protein
SPA2028-1143.221555hypothetical protein
SPA20290152.412480hypothetical protein
SPA20300142.280303hypothetical protein
SPA2031-1153.201898PTR2-family transport protein
SPA20320163.912924deoxyribodipyrimidine photolyase
SPA20330164.555719hypothetical protein
SPA20340164.621793hypothetical protein
SPA2035-1144.001639potassium-transporting ATPase subunit A
SPA2036-1153.597298P-type ATPase, high-affinity potassium transport
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2008TCRTETOQM310.004 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 31.0 bits (70), Expect = 0.004
Identities = 12/41 (29%), Positives = 23/41 (56%), Gaps = 1/41 (2%)

Query: 15 VDNAPRMQDYTLEGEEGRDM-MLLDALIQLKEKDPSLSFRR 54
++N + T+E + + MLLDAL+++ + DP L +
Sbjct: 339 IENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYV 379


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2020PF05272310.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.006
Identities = 11/33 (33%), Positives = 16/33 (48%), Gaps = 6/33 (18%)

Query: 50 KIDFTLTEGNRLALIGHNGSGKTTLLRVLAGAY 82
K D+++ L G G GK+TL+ L G
Sbjct: 594 KFDYSVV------LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2027V8PROTEASE320.002 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 31.9 bits (72), Expect = 0.002
Identities = 17/87 (19%), Positives = 27/87 (31%), Gaps = 8/87 (9%)

Query: 20 LTLVSSANIASGFHAGDAQTMLT---CVREALKNGVAIGAHPSFPDRDN--FGRT--AMV 72
+ + IASG G T+LT V + A+ A PS ++DN G +
Sbjct: 95 VEAPTGTFIASGVVVGK-DTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQI 153

Query: 73 LPPETVYAQTLYQIGALGAIVQAQGGV 99
+ + V
Sbjct: 154 TKYSGEGDLAIVKFSPNEQNKHIGEVV 180


35SPA2108SPA2114Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2108-1223.598257transcriptional regulatory protein DpiA
SPA2110-1234.857655[citrate (pro-3S)-lyase] ligase
SPA2111-2244.795475citrate lyase acyl carrier protein
SPA2112-2214.488909citrate lyase subunit beta
SPA2113-2194.311200citrate lyase subunit alpha
SPA2114-1143.214714hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2108HTHFIS644e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.7 bits (155), Expect = 4e-14
Identities = 28/138 (20%), Positives = 52/138 (37%), Gaps = 4/138 (2%)

Query: 6 TLLIVEDETLLAEMHAEYIRHIPGFKQIWLAGNLAQARMMIDRFKPGLILLDNYLPDGKG 65
T+L+ +D+ + + + + G+ + N A I L++ D +PD
Sbjct: 5 TILVADDDAAIRTVLNQALS-RAGYDVR-ITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 66 ITLLHELMQSRYPG-GVVFTTAASDMETVAEAVRSGAFDYLVKPIAYERLGQTLTRYQQR 124
LL + + P V+ +A + T +A GA+DYL KP L + R
Sbjct: 63 FDLLPRI-KKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 125 RRMLASADSASQKQIDEM 142
+ S + +
Sbjct: 122 PKRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2110LPSBIOSNTHSS381e-05 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 38.3 bits (89), Expect = 1e-05
Identities = 14/68 (20%), Positives = 31/68 (45%), Gaps = 4/68 (5%)

Query: 138 NPFTNGHRYLIQQAAAQCDWLHLFLVKEDTSRFPY---EDRLDLVLKGTTDIPRLTVHRG 194
+P T GH +I++ D +++ V + ++ P ++RL+ + K +P V
Sbjct: 10 DPITFGHLDIIERGCRLFDQVYV-AVLRNPNKQPMFSVQERLEQIAKAIAHLPNAQVDSF 68

Query: 195 SEYIISRA 202
++ A
Sbjct: 69 EGLTVNYA 76


36SPA2124SPA2146Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2124-215-4.501072hypothetical protein
SPA2125-114-3.770703alkyl hydroperoxide reductase F52A protein
SPA2126-119-4.385607alkyl hydroperoxide reductase c22 protein
SPA2127017-2.841091thiol:disulfide interchange protein DsbG
SPA2128016-2.699643putative lysR-family transcriptional regulator
SPA21290101.053888hypothetical protein
SPA2130-1132.563061hypothetical protein
SPA21310143.503361aminotransferase
SPA21320163.988575oxidoreductase
SPA21330153.953376hypothetical protein
SPA2134-1144.327617carbon starvation protein A
SPA2135-2134.585184hypothetical protein
SPA2136-2134.6829882,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
SPA2137-1125.189781isochorismatase
SPA21380115.5579222,3-dihydroxybenzoate-AMP ligase
SPA21391135.615635isochorismate synthase EntC
SPA21401133.813006ferrienterobactin-binding periplasmic protein
SPA21412154.584553membrane protein p43
SPA21422164.329841ferric enterobactin transport protein FepD
SPA21431164.130556ferric enterobactin transport protein FepG
SPA2144-1133.090673ferric enterobactin transport ATP-binding
SPA2145-1123.046156ferric enterobactin (enterochelin) transporter
SPA2146-1123.654237enterobactin synthetase component F
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2125STREPTOPAIN310.011 Streptopain (C10) cysteine protease family signature.
		>STREPTOPAIN#Streptopain (C10) cysteine protease family signature.

Length = 398

Score = 31.2 bits (70), Expect = 0.011
Identities = 17/73 (23%), Positives = 33/73 (45%), Gaps = 1/73 (1%)

Query: 2 LDTNMKTQLRAYLEKLTKPVELIATLDDS-AKSAEIKELLAEIAELSDKVTFKEDNTLPV 60
D N K + +++E + ++ LD + A +AEIK+ + + S + + + N +
Sbjct: 109 FDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHYNQGNPYNL 168

Query: 61 RKPSFLITNPGSQ 73
P PG Q
Sbjct: 169 LTPVIEKVKPGEQ 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2127BCTLIPOCALIN280.018 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 28.4 bits (63), Expect = 0.018
Identities = 18/98 (18%), Positives = 41/98 (41%), Gaps = 13/98 (13%)

Query: 30 QGITILKSFEAPGGMKGYLGKYQDMGVTIYLTPDGKHAISG--YMYNEKGENLSNALIEK 87
+ + + FE YLGK+ ++ + G ++ + N+ G ++ N
Sbjct: 21 ESVKPVSDFEL----NNYLGKWYEVARLDHSFERGLSQVTAEYRVRNDGGISVLN----- 71

Query: 88 EIYAPAGREMWQKMEKASWILDGKKDAPVVLYVFADPF 125
Y+ + W++ E ++ ++G D + + F PF
Sbjct: 72 RGYSEE-KGEWKEAEGKAYFVNGSTDGYLKVSFFG-PF 107


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2128PF05043290.033 Transcriptional activator
		>PF05043#Transcriptional activator

Length = 493

Score = 28.8 bits (64), Expect = 0.033
Identities = 30/137 (21%), Positives = 53/137 (38%), Gaps = 20/137 (14%)

Query: 7 LKKFDLNLLVIFECIYQH---LSISKAAETLYITPSAVSQSLQRLRTQFNDPLFIRSGKG 63
L K L + E +++H S+ AE L T AV L +++ F D +F S G
Sbjct: 5 LSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNG 64

Query: 64 I----TPTVTGINLHYHLENNLNSLE--QTINIMNQSSL----KKKFIIYSPQMLITQYA 113
I T +++H + + I K+ +I S + Y
Sbjct: 65 IRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSS-----SLYR 119

Query: 114 M--KLVKYIRKDPQVEI 128
+ ++ K I++ Q E+
Sbjct: 120 IISQINKVIKRQFQFEV 136


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2136DHBDHDRGNASE338e-120 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 338 bits (868), Expect = e-120
Identities = 104/257 (40%), Positives = 147/257 (57%), Gaps = 20/257 (7%)

Query: 9 KTVWVTGAGKGIGYATALAFVDAGARVIGFDRE---------------FTQENYPFATEV 53
K ++TGA +GIG A A GA + D E +P
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP----- 63

Query: 54 MDVADAGQVAQVCQRVLQKTPRLDVLVNAAGILRMGATDALSVDDWQQTFAVNVGGAFNL 113
DV D+ + ++ R+ ++ +D+LVN AG+LR G +LS ++W+ TF+VN G FN
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 114 FSQTMAQFRRQQGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALTVGLELAGCGVRCN 173
++ G+IVTV S+ A PR M+AY +SKAA +GLELA +RCN
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 174 VVSPGSTDTDMQRTLWVSEDAEQQRIRGFGEQFKLGIPLGKIARPQEIANTILFLASDLA 233
+VSPGST+TDMQ +LW E+ +Q I+G E FK GIPL K+A+P +IA+ +LFL S A
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 234 SHITLQDIVVDGGSTLG 250
HIT+ ++ VDGG+TLG
Sbjct: 244 GHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2137ISCHRISMTASE425e-154 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 425 bits (1095), Expect = e-154
Identities = 148/299 (49%), Positives = 192/299 (64%), Gaps = 18/299 (6%)

Query: 1 MAIPKLQSYALPTALDIPTNKVNWAFEPERAALLIHDMQDYFVSFWGRNCPMMDQVIANI 60
MAIP +Q Y +PTA D+P NKV+W +P RA LLIHDMQ+YFV + + ++ ANI
Sbjct: 1 MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI 60

Query: 61 AALRQYCKEHHIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVEALTPDEADTV 120
L+ C + IPV YTAQP Q+ +DRALL D WGPGL P ++K++ L P++ D V
Sbjct: 61 RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV 120

Query: 121 LVKWRYSAFHRSPLEQMLKDTGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD 180
L KWRYSAF R+ L +M++ GR+QLIITG+YAHIGC+ TA +AFM DIK F V DA+AD
Sbjct: 121 LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD 180

Query: 181 FSREEHLMALNYVAGRSGRVVMTESLL------PTPVPASKA-----------ALRALIL 223
FS E+H MAL Y AGR VMT+SLL P V + A +R I
Sbjct: 181 FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA 240

Query: 224 PLLDETDEPLD-DENLIDYGLDSVRMMGLAARWRKVHGDIDFVMLAKNPTIDAWWALLS 281
LL ET E + E+L+D GLDSVR+M L +WR+ ++ FV LA+ PTI+ W LL+
Sbjct: 241 ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLT 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2140FERRIBNDNGPP594e-12 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 58.8 bits (142), Expect = 4e-12
Identities = 46/210 (21%), Positives = 81/210 (38%), Gaps = 21/210 (10%)

Query: 105 EPNAETVAAQMPDLILISATGGDSALALYDQLSAIAPTLVINYDDKS-----WQSLLTQL 159
EPN E + P ++ SA G S + L+ IAP N+ D + LT++
Sbjct: 86 EPNLELLTEMKPSFMVWSAGYGPS----PEMLARIAPGRGFNFSDGKQPLAMARKSLTEM 141

Query: 160 GEITGQEKQAAARIAEFEAQLTTVKQRIALPPQPVSALVYTPAAHSANLWTPESTQGKLL 219
++ + A +A++E + ++K R L ++ P S ++L
Sbjct: 142 ADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEIL 201

Query: 220 TQVGFTLATLPQGLQTSKSQGKRHDIIQLGGENLAAGLNGESLFLFAGDNNDVAALYANP 279
+ G A + + + + LAA + + L ++ D+ AL A P
Sbjct: 202 DEYGIPNAW--------QGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATP 253

Query: 280 LLAHLPAVQNKRVYALGTETFRLDYYSATL 309
L +P V+ R + F Y ATL
Sbjct: 254 LWQAMPFVRAGRFQRVPAVWF----YGATL 279


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2141TCRTETB290.039 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.1 bits (65), Expect = 0.039
Identities = 69/394 (17%), Positives = 130/394 (32%), Gaps = 60/394 (15%)

Query: 27 FISIVSLGLLGVAVPVQIQMMTHSTWQVGLSVTLTGGAMFIGLMVGGVLADRYERKKVIL 86
F S+++ +L V++P T IG V G L+D+ K+++L
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 87 LARGTCGIGFIGLCVNALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQ 146
G + V ++A ++ G F +L ++ + +EN +
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPAL----VMVVVARYIPKENRGK 139

Query: 147 AGAITMLTVRLGSVISPMLGGILLASGGVAWNYGLAAAGTFITLLPLLTLPRLPVPPQPR 206
A + V +G + P +GG++ + W+Y L IT++ + L +L
Sbjct: 140 AFGLIGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIP--MITIITVPFLMKLLKKEVRI 195

Query: 207 ENP--------------------------FLALLAAFRFLLA------------------ 222
+ FL + +
Sbjct: 196 KGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKN 255

Query: 223 CPLIGGIALLGGLVTMASAVRVLYPALAMS--WQMSAAQIGLLYAAI-PLGAAIGALTSG 279
P + G+ L GG++ A V M Q+S A+IG + + I G
Sbjct: 256 IPFMIGV-LCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGG 314

Query: 280 QLAHSVRPGLIMLVSTVG---SFLAVGLFAIMPVWIAGVICLALFGWLSAISSLLQYTLL 336
L P ++ + SFL W +I + + G LS +++ +
Sbjct: 315 ILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVS 374

Query: 337 QTQTPENMLGRMNGLWTAQNVTGDAIGAALLGGL 370
+ + M L + + G A++GGL
Sbjct: 375 SSLKQQEAGAGM-SLLNFTSFLSEGTGIAIVGGL 407


37SPA2165SPA2198Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2165029-7.614555inner membrane proton/cation antiporter
SPA2166233-9.544354copper-binding protein
SPA2167132-9.162340bactoprenol-linked glucose translocase
SPA2168233-10.111293bactoprenol glucosyltransferase
SPA2170028-7.795800transposase
SPA2173023-6.173235*fimbriae w protein
SPA2174215-1.958505hypothetical protein
SPA2175215-1.673987fimbriae Y protein
SPA2176316-0.795800transcriptional regulator)
SPA21772140.662849fimbria-like protein FimF precursor
SPA21782140.678662FimH protein
SPA21791131.201242outer membrane usher protein FimD
SPA2180-1140.855459fimbrial chaperone protein
SPA2181-1121.738580hypothetical protein
SPA2182-1141.136058type-1 fimbrial protein subunit A
SPA21830151.350503bifunctional methylenetetrahydrofolate
SPA21840182.107089hypothetical protein
SPA21850172.799021hypothetical protein
SPA21860153.119014cysteinyl-tRNA synthetase
SPA21870103.637533peptidyl-prolyl cis-trans isomerase B
SPA21881124.358331hypothetical protein
SPA21891113.146832phosphoribosylaminoimidazole carboxylase
SPA21900122.240572phosphoribosylaminoimidazole carboxylase ATPase
SPA21910130.862185carbamate kinase
SPA21921140.969118hypothetical protein
SPA21932140.133108hypothetical protein
SPA2195114-0.921316ureidoglycolate dehydrogenase
SPA2196113-1.611055allantoate amidohydrolase
SPA2197114-2.031216hypothetical protein
SPA2198217-2.528389glycerate kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2165ACRIFLAVINRP537e-12 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 52.9 bits (127), Expect = 7e-12
Identities = 13/37 (35%), Positives = 25/37 (67%)

Query: 25 GAGSEVMSRIATPMIGGMITAPLLSLFIIPAAYKLMR 61
GAGS + + ++GGM++A LL++F +P + ++R
Sbjct: 993 GAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2170HTHFIS342e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 33.7 bits (77), Expect = 2e-05
Identities = 7/45 (15%), Positives = 17/45 (37%), Gaps = 1/45 (2%)

Query: 4 KRYPEEFKIEAVRQVVER-GHSVSSVATHLDITTHSLYARIKKYG 47
R E + + + + A L + ++L +I++ G
Sbjct: 430 DRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELG 474


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2176HTHFIS702e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 70.2 bits (172), Expect = 2e-16
Identities = 29/122 (23%), Positives = 58/122 (47%), Gaps = 2/122 (1%)

Query: 1 MKPASVIIMDEHPIVRMSIEVLLGKNSNIQVVLKTDDSRTAIEYLRTYPVDLVILDIELP 60
M A++++ D+ +R + L + V T ++ T ++ DLV+ D+ +P
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYD--VRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 GTDGFTLLKRIKSIQEHTRILFLSSKSEAFYAGRAIRAGANGFVSKRKDLNDIYNAVKMI 120
+ F LL RIK + +L +S+++ A +A GA ++ K DL ++ +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 121 LS 122
L+
Sbjct: 119 LA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2179PF005778260.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 826 bits (2134), Expect = 0.0
Identities = 406/856 (47%), Positives = 561/856 (65%), Gaps = 19/856 (2%)

Query: 21 VALSVLAALCPLTSRGESYFNPAFLSADTASVADLSRFEKGYHQPPGIYRVDIWRNDEFV 80
+ ++ A S E YFNP FL+ D +VADLSRFE G PPG YRVDI+ N+ ++
Sbjct: 30 LFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYM 89

Query: 81 ATQDIRFEAGAVGAGDKSGGLMPCFTPEWIKRLGVNTAVFPVSDKGVDTSCIHLPEKIPG 140
AT+D+ F G D G++PC T + +G+NTA + D +C+ L I
Sbjct: 90 ATRDVTFNTG-----DSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHD 144

Query: 141 AEVAFDFASMRLNISLPQASLLNSARGYIPPEEWDEGIPAALINYSFTGSR-----GTDS 195
A D RLN+++PQA + N ARGYIPPE WD GI A L+NY+F+G+ G +S
Sbjct: 145 ATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNS 204

Query: 196 DSYFLSLLSGLNYGPWRLRNNGAWNYSKGDG--YHSQRWNNIGTWVQRAIIPLKSELVMG 253
+L+L SGLN G WRLR+N W+Y+ D +W +I TW++R IIPL+S L +G
Sbjct: 205 HYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLG 264

Query: 254 DSNTGNDVFDSVGFRGARLYSSDNMYPDSLQGYAPTVRGIARTAAKLTIRQNGYVIYQNY 313
D T D+FD + FRGA+L S DNM PDS +G+AP + GIAR A++TI+QNGY IY +
Sbjct: 265 DGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNST 324

Query: 314 VSPGAFAITDLNPTSSSGDLEVTVDEKDGSQQRYTVPYSTVPLLQREGRVKYDLVAGDFR 373
V PG F I D+ +SGDL+VT+ E DGS Q +TVPYS+VPLLQREG +Y + AG++R
Sbjct: 325 VPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYR 384

Query: 374 SGNSQQSSPFFFQGTVIAGLPAGLTAYGGTQLADRYRAVVVGAGQNLGDWGAVSVDVTHA 433
SGN+QQ P FFQ T++ GLPAG T YGGTQLADRYRA G G+N+G GA+SVD+T A
Sbjct: 385 SGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQA 444

Query: 434 RSQLADDSTHQGQSLRFLYAKSLNNYGTNFQLLGYRYSTRGFYTLDDVAYRSMEGYDYEY 493
S L DDS H GQS+RFLY KSLN GTN QL+GYRYST G++ D Y M GY+ E
Sbjct: 445 NSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIE- 503

Query: 494 DSDGRRHKVPVAQSYHNLRYSKKGRFQVNISQNLGDYGSLYLSGSQQNYWNTADTNTWYQ 553
DG P Y+NL Y+K+G+ Q+ ++Q LG +LYLSGS Q YW T++ + +Q
Sbjct: 504 TQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQ 563

Query: 554 LGYASGWQDISYSLSWSWNESVGISGADRILAFNMSAPFSVLTGRRYARDTILDRTYATF 613
G + ++DI+++LS+S ++ G D++LA N++ PFS R + A++
Sbjct: 564 AGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFS--HWLRSDSKSQWRHASASY 621

Query: 614 NANRNRDGDNSWQSGVGGTLLEGRNLSYSVTQGRS----STNGYSGSASASWQATYGTLG 669
+ + + +G + +GV GTLLE NLSYSV G + +G +G A+ +++ YG
Sbjct: 622 SMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNAN 681

Query: 670 VGYNYDRDQHDYNWQLSGGVVGHADGITFSQPLGDTNVLIKAPGAKGVRIENQTGVKTDW 729
+GY++ D + +SGGV+ HA+G+T QPL DT VL+KAPGAK ++ENQTGV+TDW
Sbjct: 682 IGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDW 741

Query: 730 RGYAVMPYATVYRYNRVALDTNTMDNHTDVENNVSSVVPTEGALVRAAFDTRIGVRAIIT 789
RGYAV+PYAT YR NRVALDTNT+ ++ D++N V++VVPT GA+VRA F R+G++ ++T
Sbjct: 742 RGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMT 801

Query: 790 ARLGGRPLPFGAIVRETASGITSMVGDDGQIYLSGLPLKGELFIQWGEGKNARCIAPYAL 849
+PLPFGA+V +S + +V D+GQ+YLSG+PL G++ ++WGE +NA C+A Y L
Sbjct: 802 LTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQL 861

Query: 850 AENSLKQAITIVSATC 865
S +Q +T +SA C
Sbjct: 862 PPESQQQLLTQLSAEC 877


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2186RTXTOXIND300.018 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.2 bits (68), Expect = 0.018
Identities = 16/149 (10%), Positives = 42/149 (28%), Gaps = 8/149 (5%)

Query: 299 RSQLNYSEENLKQARASLERLYTALRGTDKSAAPAGGEAFEARFVEAMNDDFNTPEAY-- 356
+ ++ +L QAR R R + + P E F ++ +
Sbjct: 133 EADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIK 192

Query: 357 SVLFDMAREVN--RLKGEDMTAA-NAMASHLRKISGVLGLLEQEPDVFLQSGAQADDGEV 413
+ L + A + + + + + + + D F +
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSS---LLHKQAI 249

Query: 414 AEIEALIQQRLDARKAKDWAAADAARDRL 442
A+ L Q+ + + +++
Sbjct: 250 AKHAVLEQENKYVEAVNELRVYKSQLEQI 278


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2191CARBMTKINASE359e-127 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 359 bits (923), Expect = e-127
Identities = 126/310 (40%), Positives = 176/310 (56%), Gaps = 16/310 (5%)

Query: 2 KTLVVALGGNALLQRGEALTAENQYRNIADAVPALARL-ARSYRLAIVHGNGPQVGLLAL 60
K +V+ALGGNAL QRG+ + E N+ +A + AR Y + I HGNGPQVG L L
Sbjct: 3 KRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSLLL 62

Query: 61 QNLAWKA---VEPYPLDVLVAESQGMIGYMLAQRLALEPDM----PPVTAVLTRIKVSAD 113
A +A + P+DV A SQG IGYM+ Q L E V ++T+ V +
Sbjct: 63 HMDAGQATYGIPAQPMDVAGAMSQGWIGYMIQQALKNELRKRGMEKKVVTIITQTIVDKN 122

Query: 114 DPAFLEPEKFIGPVYSPEEQMSLEATYGWHMKRD-GKYLRRVVASPAPRQIIESAAIELL 172
DPAF P K +GP Y E L GW +K D G+ RRVV SP P+ +E+ I+ L
Sbjct: 123 DPAFQNPTKPVGPFYDEETAKRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETIKKL 182

Query: 173 LKEGHVVICSGGGGVPVAGEG---EGVEAVIDKDLAAALLAEQIAADGLIILTDADAVYE 229
++ G +VI SGGGGVPV E +GVEAVIDKDLA LAE++ AD +ILTD +
Sbjct: 183 VERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNGAAL 242

Query: 230 HWGTPQQRAIRQASPDELAPFAKAD----GAMGPKVTAVSGYVKRCGKPAWIGALSRIDD 285
++GT +++ +R+ +EL + + G+MGPKV A +++ G+ A I L + +
Sbjct: 243 YYGTEKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWGGERAIIAHLEKAVE 302

Query: 286 TLAGRAGTCI 295
L G+ GT +
Sbjct: 303 ALEGKTGTQV 312


38SPA2270SPA2293Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2270424-0.587938peptidyl-prolyl cis-trans isomerase D
SPA2271426-0.634898DNA-binding protein HU-beta
SPA2272424-0.516182Lon protease
SPA2273320-0.111749ATP-dependent clp protease ATP-binding subunit
SPA2274118-0.497437ATP-dependent clp protease proteolytic subunit
SPA2275021-0.001947trigger factor
SPA2276-1200.199938BolA protein
SPA22770220.310419lipoprotein
SPA22780220.335025AmpG protein
SPA2279220-0.854049cytochrome o ubiquinol oxidase subunit II
SPA2280-116-2.769703cytochrome o ubiquinol oxidase subunit I
SPA2281018-4.095542cytochrome o ubiquinol oxidase subunit III
SPA2282016-3.251108cytochrome o ubiquinol oxidase C subunit
SPA2283016-3.254708cytochrome o ubiquinol oxidase C subunit
SPA2284-119-3.317736hypothetical protein
SPA2285-216-2.715701hypothetical protein
SPA2286-2141.599365transposase
SPA2287-1141.890745hypothetical major facilitator family transport
SPA2288-1182.695205hypothetical protein
SPA22890183.286727ApbA protein
SPA22900184.3492554-methyl-5(b-hydroxyethyl)-thiazole
SPA22911184.402281phosphonoacetaldehyde phosphonohydrolase
SPA22922193.9915222-aminoethylphosphonate--pyruvate transaminase
SPA22931213.104569regulator of 2-aminoethylphosphonate uptake and
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2271DNABINDINGHU1158e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 115 bits (291), Expect = 8e-38
Identities = 49/88 (55%), Positives = 67/88 (76%)

Query: 2 NKSQLIEKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTGR 61
NK LI K+A +++K + A+DA+ ++V+ L +G+ V L+GFG F V+ERAAR GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEITIAAAKVPSFRAGKALKDAV 89
NPQTG+EI I A+KVP+F+AGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2272GPOSANCHOR340.002 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 34.3 bits (78), Expect = 0.002
Identities = 34/133 (25%), Positives = 68/133 (51%), Gaps = 15/133 (11%)

Query: 191 ERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDAPD- 249
LE A +E + +L R +++ ++ S+ +Q++A ++L E + +
Sbjct: 291 AALEAEKADLEHQSQVLNAN---RQSLRRDLDASREAK---KQLEAEHQKLEEQNKISEA 344

Query: 250 ENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSPMS-AEATVVRGYIDWMVQVPWNARSK 308
++L+R +DA++ EAK++ EAE QKL+ + +S A +R +D + A+ +
Sbjct: 345 SRQSLRRDLDASR---EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASRE----AKKQ 397

Query: 309 VKKDLRQAQEILD 321
V+K L +A L
Sbjct: 398 VEKALEEANSKLA 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2278TCRTETB432e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 42.9 bits (101), Expect = 2e-06
Identities = 40/190 (21%), Positives = 76/190 (40%), Gaps = 15/190 (7%)

Query: 221 RNNAWLI-LLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLSLLATIVGALYG 279
R+N LI L ++ + + + ++++ + VN L +I A+YG
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 280 GILMQRLSLFRALLIFGILQGASNAGYWLLSITDKNMFSMGAAVFFENLCGGMGTAAFVA 339
L +L + R LL I+ + ++ + FS+ + G G AAF A
Sbjct: 71 K-LSDQLGIKRLLLFGIIINCFGS----VIGFVGHSFFSL---LIMARFIQGAGAAAFPA 122

Query: 340 LLM----TLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAH-GWPTFYLFSVVAAVP 394
L+M K F L+ ++ A+G VGP G + + W L ++ +
Sbjct: 123 LVMVVVARYIPKENRGKAFGLIGSIVAMG-EGVGPAIGGMIAHYIHWSYLLLIPMITIIT 181

Query: 395 GLLLLLVCRQ 404
L+ + ++
Sbjct: 182 VPFLMKLLKK 191


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2287TCRTETA841e-19 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 84.1 bits (208), Expect = 1e-19
Identities = 85/382 (22%), Positives = 156/382 (40%), Gaps = 26/382 (6%)

Query: 17 LGTVFSLRMLGMFMVLPVLTTY--GMALQSASEALIGIAIGIYGLAQAIFQIPFGLLSDR 74
L TV L +G+ +++PVL + + A GI + +Y L Q G LSDR
Sbjct: 11 LSTVA-LDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDR 69

Query: 75 IGRKPLIVGGLAVFVAGSIIAALSHSIWGIILGRALQG-SGAIAAAVMALLSDLTREQNR 133
GR+P+++ LA I A + +W + +GR + G +GA A A ++D+T R
Sbjct: 70 FGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDER 129

Query: 134 TKAMAFIGVSFGITFAIAMVLGPIVTHSLGLNALFWMIAALATLGILLTIWVVPNSTNHV 193
+ F+ FG VLG ++ +A F+ AAL L L +++P S
Sbjct: 130 ARHFGFMSACFGFGMVAGPVLGGLMG-GFSPHAPFFAAAALNGLNFLTGCFLLPESHKGE 188

Query: 194 LNRESGMVKGSFSKVLAEPRLLKLNFGIMCLHILLMSTFVA-LPGQLADAGFPAAEHWKV 252
+ + G+ + L+ F+ L GQ+ A + +
Sbjct: 189 RRPLRREALNPLAS-------FRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRF 241

Query: 253 YLATMVIAFA--------AVVPFIIYAEVKRRMKQVFLFCVGLI--VVAEIVLWGAGQHF 302
+ I + ++ +I V R+ + +G+I I+L A + +
Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGW 301

Query: 303 WELVIGVQLFFLAFNL--MEALLPSLISKESPAGYKGTAMGVYSTSQFLGVALGGSLGGW 360
I V L + ++A+L + +E +G+ + S + +G L ++
Sbjct: 302 MAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAA 361

Query: 361 IDGTFDGQTVFLAGAVLAMVWL 382
T++G ++AGA L ++ L
Sbjct: 362 SITTWNG-WAWIAGAALYLLCL 382


39SPA2364SPA2428Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2364013-3.594437hypothetical protein
SPA2365013-4.053456DNA restriction (DNA helicase
SPA2366015-4.822626type III restriction-modification system StyLTI
SPA2367-220-4.820800metabolite transport protein
SPA2369031-9.276445hypothetical protein
SPA2370230-8.874990lipoprotein
SPA2371233-9.989273secreted protein
SPA2372234-10.745314transcriptionl regulator
SPA2373335-9.969570outer membrane protein
SPA2375333-9.442132transmembrane regulator
SPA2376323-5.242732rtn protein
SPA2377422-4.569068hypothetical protein
SPA2378322-3.983631transmembrane regulator
SPA2379321-3.291545fimbrial protein
SPA2380321-3.289545fimbrial chaperone protein
SPA2381120-3.788200outer membrane fimbrial usher protein
SPA2382134-10.884422fimbrial protein
SPA2383442-12.777612fimbrial chaperone protein
SPA2384547-13.610340secreted protein
SPA2385446-10.416798O-antigen conversion: glucosyl transferase
SPA2386545-9.225495O-antigen conversion: bactoprenyl transferase
SPA2387646-8.231454O-antigen conversion: translocase
SPA2388542-5.064371tailspike
SPA2389941-3.055075regulatory
SPA2390940-2.944720DNA transfer protein
SPA2391836-2.813016DNA transfer protein
SPA2392935-3.515976DNA transfer protein
SPA2393833-4.232136hypothetical protein
SPA2394736-3.969569portal protein
SPA2395639-4.342859hypothetical protein
SPA2396538-4.303496hypothetical protein
SPA2397340-4.527342lysozyme
SPA2398340-3.438571lysis (holin)
SPA2399340-4.520574antitermination
SPA2400441-5.539205hypothetical protein
SPA2401339-5.659055hypothetical protein
SPA2402737-4.917193hypothetical protein
SPA2403637-6.832108hypothetical protein
SPA2404640-7.208621hypothetical protein
SPA2405636-4.394577hypothetical protein
SPA2406536-5.264234hypothetical protein
SPA2407635-5.445800hypothetical protein
SPA2408635-5.856906hypothetical protein
SPA2409736-6.102580hypothetical protein
SPA2410635-6.062653DNA replication (helicase)
SPA2411739-8.826385DNA replication
SPA2412841-9.251833hypothetical protein
SPA2413840-9.726103transcriptional activator-regulatory protein
SPA2414840-9.551619transcriptional activator-regulatory protein
SPA2415739-9.164936hypothetical protein
SPA24161038-9.736969superinfection exclusion
SPA24171240-8.353051hypothetical protein
SPA24181037-7.545221antirestriction protein
SPA2419742-7.742029hypothetical protein
SPA2420438-7.863684hypothetical protein
SPA2421538-8.296282hypothetical protein
SPA2422435-6.807249hypothetical protein
SPA2423536-4.734209hypothetical protein
SPA2424435-4.438933hypothetical protein
SPA2425733-3.362467hypothetical protein
SPA2426936-3.830787hypothetical protein
SPA2427524-1.833805hypothetical protein
SPA2428320-0.718010hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2367TCRTETA561e-10 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 55.6 bits (134), Expect = 1e-10
Identities = 56/306 (18%), Positives = 108/306 (35%), Gaps = 17/306 (5%)

Query: 19 FTSWMLDAFDFFILVFVLSDLAEWFHAS---VSDVSIAIMLTLAVRPIGALLFGRMAEKY 75
++ LDA +++ VL L S + I + L ++ A + G +++++
Sbjct: 11 LSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRF 70

Query: 76 GRRPILMLNILFFTVFELLSAWSPTFMAFLIFRVMYGVAMGGIWGVASSLAMETIPDRSR 135
GRRP+L++++ V + A +P I R++ G+ G VA + + R
Sbjct: 71 GRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDER 129

Query: 136 ----GLMSGIFQAGYPCGYLFASVIFGLFYSMVGWRGMFLIGA---LPVVLLPYIWFKVP 188
G MS F G + A + G F A L
Sbjct: 130 ARHFGFMSACFGFG-----MVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPES 184

Query: 189 ESPVWLAARARKENTALLPVLRKQWKLCLYLVLVMAFFNFFSHGTQDLYPTFLKMQHSFD 248
R N + + L+ V L+ F + + +D
Sbjct: 185 HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWD 244

Query: 249 PHLISI-IAIFYNIAAMLGGIFYGTLSERIGRKKAIMIAAFLALPVLPLWAFSSGSFTIG 307
I I +A F + ++ + G ++ R+G ++A+M+ L AF++ +
Sbjct: 245 ATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAF 304

Query: 308 LGAFLM 313
L+
Sbjct: 305 PIMVLL 310



Score = 33.6 bits (77), Expect = 0.001
Identities = 37/186 (19%), Positives = 77/186 (41%), Gaps = 10/186 (5%)

Query: 3 TPLNWTTTQRHVAFASFTSWMLDAF-DFFILVFVLSDLAEWFHASVSDVSIAIMLTLAVR 61
W VA +++ ++V+ + FH + + I++ +
Sbjct: 201 ASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG-EDRFHWDATTIGISLAAFGILH 259

Query: 62 PIG-ALLFGRMAEKYGRRPILMLNILF-FTVFELLSAWSPTFMAFLIFRVMYGVAMG--G 117
+ A++ G +A + G R LML ++ T + LL+ + +MAF I ++ +G
Sbjct: 260 SLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPA 319

Query: 118 IWGVASSLAMETIPDRSRGLMSGIFQAGYPCGYLFASVIFGLFYSMVGWRG-MFLIGA-L 175
+ + S E + +G ++ + G L + I+ S+ W G ++ GA L
Sbjct: 320 LQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYA--ASITTWNGWAWIAGAAL 377

Query: 176 PVVLLP 181
++ LP
Sbjct: 378 YLLCLP 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2373ENTEROVIROMP1347e-43 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 134 bits (339), Expect = 7e-43
Identities = 59/183 (32%), Positives = 88/183 (48%), Gaps = 21/183 (11%)

Query: 1 MKRRSSFLVFLGLLLASPLALANDQHTVSFGYAQTHLSSLKNSDSKDLRGFNFKYRYEFN 60
MK+ + +L + TV+ GYAQ+ N + GFN KYRYE +
Sbjct: 1 MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMN----KMGGFNLKYRYEED 56

Query: 61 ET-WGMLGSFTATRNEMENYTWKEGKLHKNGSDSVDYGSLMFGPTYRFNDYVSLYGNAGI 119
+ G++GSFT T + K Y + GP YR ND+ S+YG G+
Sbjct: 57 NSPLGVIGSFTYTEKSRTASSGDYNK--------NQYYGITAGPAYRINDWASIYGVVGV 108

Query: 120 ATMKF--------NKHSKEDSFAYGAGVIFNPVKSISIDASWEASRFFAVDTNTFGVSVG 171
KF + + F+YGAG+ FNP++++++D S+E SR +VD T+ VG
Sbjct: 109 GYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIAGVG 168

Query: 172 YRF 174
YRF
Sbjct: 169 YRF 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2381PF005777650.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 765 bits (1977), Expect = 0.0
Identities = 261/880 (29%), Positives = 413/880 (46%), Gaps = 63/880 (7%)

Query: 4 TINLNRKS-LALLIAIVCSGSAQG----EEYYFDPALLQGATYGQ-NIARFNE-QQTPSG 56
I +R + + + + C+ +AQ E YF+P L +++RF Q+ P G
Sbjct: 17 HIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPG 76

Query: 57 DYLADVYVNGTLVTASTNIRFNAVKEGQQAEPCLPLSVMKAAQIKSLPETDAA----TEC 112
Y D+Y+N + A+ ++ FN Q PCL + + + + + + C
Sbjct: 77 TYRVDIYLNNGYM-ATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDAC 135

Query: 113 RPLREWVPHAGWQFDSATLRLLLTIPMTELTHKPRGYISPSEWDSGALALFLRHNTNWTH 172
PL + A Q D RL LTIP ++++ RGYI P WD G A L +N +
Sbjct: 136 VPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNS 195

Query: 173 TENTDSHYRYQYLWSGLNMGVNLGLWQVRHQSNLRYANSNQS-GSAWRYNSVRTWVQRPV 231
+N Y + L G+N+G W++R + Y +S+ S GS ++ + TW++R +
Sbjct: 196 VQN-RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDI 254

Query: 232 ASINSILSLGDSYTDSSLFGSLSFNGVKLVTDERMRPQGKRGYAPEVRGVAASSAHVVVK 291
+ S L+LGD YT +F ++F G +L +D+ M P +RG+AP + G+A +A V +K
Sbjct: 255 IPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIK 314

Query: 292 QLGKVIYETNVPPGPFYIDDLYNTRYQGDLEVEVIEASGKTSRFTVPYSSVPDSVRPGNW 351
Q G IY + VPPGPF I+D+Y GDL+V + EA G T FTVPYSSVP R G+
Sbjct: 315 QNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHT 374

Query: 352 HYSLAFGRVRQYY--DIENRFFEGTFQHGVNNTITLNLGSRIAQRYQAWLAGGVWATGM- 408
YS+ G R + RFF+ T HG+ T+ G+++A RY+A+ G G
Sbjct: 375 RYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGAL 434

Query: 409 GAFGLNATWSNARAEHNERQQGWRAELSYSKTFT-TGTNLVLAAYRYSTNGFRDLQDVLG 467
GA ++ T +N+ + + G Y+K+ +GTN+ L YRYST+G+ + D
Sbjct: 435 GALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTY 494

Query: 468 VRREAKTGI-------------DYYSDTLHQRNRLSATVSQPLGRLGTLNLSASTADYYN 514
R DYY+ ++R +L TV+Q LGR TL LS S Y+
Sbjct: 495 SRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWG 554

Query: 515 NQSRITQLQMGYSNQWRNISYGVNIARQRTTWDYDRFYHGVNEPLDVSSRQKYTETTMSF 574
+ Q Q G + + +I++ ++ + + W + ++
Sbjct: 555 TSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKG------------------RDQMLAL 596

Query: 575 NVSIPLDWGENRTSVA------MNYNQSSQSRSST---VSMTGSSGENSDLSWSVYGGYE 625
NV+IP S + +Y+ S + G+ E+++LS+SV GY
Sbjct: 597 NVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYA 656

Query: 626 RYRNSNSDSSAPTTFGGNLQQNTRFGALRANYDQGDNYRQEGLGASGTLVLHPGGLTAGP 685
+ NS S+ L +G Y D+ +Q G SG ++ H G+T G
Sbjct: 657 GGGDGNSGSTG----YATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQ 712

Query: 686 YTSDTFALIHADGAQGAIVQNGQGAVVDRFGYAILPSLSPYRINNVTLDTRKMRSDAELT 745
+DT L+ A GA+ A V+N G D GYA+LP + YR N V LDT + + +L
Sbjct: 713 PLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLD 772

Query: 746 GGSQQIVPYAGAIARVNFATISGKAVLISVKMPDGGIPPMGADVFNGEGTNIGMVGQSGQ 805
+VP GAI R F G +L+++ + P GA V + + G+V +GQ
Sbjct: 773 NAVANVVPTRGAIVRAEFKARVGIKLLMTLT-HNNKPLPFGAMVTSESSQSSGIVADNGQ 831

Query: 806 IYARIAHPSGSLLVRWGTGANQRCRVAYQLDLHTKEPFLY 845
+Y +G + V+WG N C YQL +++ L
Sbjct: 832 VYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLT 871


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2388PF01540340.002 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

Length = 475

Score = 33.9 bits (77), Expect = 0.002
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 9/59 (15%)

Query: 94 DANGSQVDYIANVLKYDPDQYSI---------EADKKFKYSVKLSDYPTLQDAASAAVD 143
DA Q + +A LK +PD I EA K FK + DYP + SAAV+
Sbjct: 42 DAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYGDYPAIISKLSAAVE 100


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2400HTHFIS270.005 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.7 bits (59), Expect = 0.005
Identities = 11/28 (39%), Positives = 14/28 (50%)

Query: 6 QTIPELLIQTRGNQTEVARMLSCARGTV 33
I L TRGNQ + A +L R T+
Sbjct: 439 PLILAALTATRGNQIKAADLLGLNRNTL 466


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2410DNABINDINGHU310.002 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 31.2 bits (71), Expect = 0.002
Identities = 10/49 (20%), Positives = 20/49 (40%), Gaps = 3/49 (6%)

Query: 123 MTEATEL---LYSRNGMTATQKYEAIQAIFTQLTDHAKTGSRRGLRSFG 168
M +L + +T A+ A+F+ ++ + G + L FG
Sbjct: 1 MANKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFG 49


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2424HTHFIS260.027 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 25.9 bits (57), Expect = 0.027
Identities = 8/22 (36%), Positives = 13/22 (59%)

Query: 22 RAAEHLGLNINQFYYIAKKLSL 43
+AA+ LGLN N ++L +
Sbjct: 454 KAADLLGLNRNTLRKKIRELGV 475


40SPA2445SPA2501Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2445-215-3.009044phosphoheptose isomerase
SPA2446019-3.302913acyl-CoA dehydrogenase
SPA2447827-4.032402hydrolase
SPA24481129-4.846947secreted protein
SPA24491130-4.734638outer membrane adhesin
SPA24511129-3.880382transcriptional regulator
SPA24521228-2.478696fimbrail protein
SPA24531227-1.694724outermembrane fimbrial usher protein
SPA2454935-7.494698fimbrial subunit
SPA2455834-6.314848fimbrial protein
SPA2456929-2.817012hypothetical protein
SPA2457931-3.296591hypothetical protein
SPA2458933-4.089417hypothetical protein
SPA2459832-4.328651LysR-family transcriptional regulator SinR
SPA2460931-2.421687ybeJ-like protein
SPA2462832-2.723982outer-membrane fimbrial usher protein
SPA2463638-9.700495periplasmic fimbrial chaperone protein
SPA24645320.303750lipoprotein
SPA24654281.429401hypothetical protein
SPA24664270.243679hypothetical protein
SPA2467426-1.239417hypothetical protein
SPA2469425-1.912884hypothetical protein
SPA2470425-1.412260Rhs-family protein
SPA2471632-7.518846hypothetical protein
SPA2472541-8.227891hypothetical protein
SPA24730222.271751hypothetical protein
SPA24740222.667218secreted protein
SPA24751222.094760hypothetical protein
SPA24761223.493325secreted protein
SPA24781244.593686hypothetical protein
SPA24790223.501704inner membrane protein
SPA2480020-1.250946shiga-like toxin A subunit
SPA2481019-0.712363hypothetical protein
SPA24821200.138194hypothetical protein
SPA2483220-2.080416hypothetical protein
SPA2484225-5.525929lipoprotein
SPA2485527-7.158279hypothetical protein
SPA2486630-8.486691hypothetical protein
SPA2487117-2.822722hypothetical protein
SPA2488-118-0.729198hypothetical protein
SPA2489-116-1.750545hypothetical protein
SPA2490-116-1.966397hypothetical protein
SPA2491-1132.352365hypothetical protein
SPA2492-1132.218839hypothetical protein
SPA2493-1153.246345hypothetical protein
SPA24940163.407663hypothetical protein
SPA24950175.041242hypothetical protein
SPA24960175.461062ClpB-like protein
SPA24972205.892612hypothetical protein
SPA24991205.439407hypothetical protein
SPA25000184.690567hypothetical protein
SPA25010154.017386hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2449ENTEROVIROMP335e-04 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 33.0 bits (75), Expect = 5e-04
Identities = 14/62 (22%), Positives = 26/62 (41%), Gaps = 7/62 (11%)

Query: 146 VGLAHVKLSNNTIPVGFGINETLSASKNNFAWGAGIGAKYAVTDNIMIDASYKYINAGKV 205
VG+ + K P S F++GAG+ ++ +N+ +D SY+ V
Sbjct: 106 VGVGYGKFQTTEYPTYKH-----DTSDYGFSYGAGL--QFNPMENVALDFSYEQSRIRSV 158

Query: 206 SI 207
+
Sbjct: 159 DV 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2453PF00577694e-14 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 69.1 bits (169), Expect = 4e-14
Identities = 98/658 (14%), Positives = 194/658 (29%), Gaps = 92/658 (13%)

Query: 78 PAAERQKALAALSRPLLRNSNLVCGVSEAK-------DSSECGYVATDKEDVAVIFDENN 130
Q + L+R L + G++ A C + + D D
Sbjct: 98 TGDSEQGIVPCLTRAQLASM----GLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQ 153

Query: 131 AQLSLFLNRDWLPDEERRDKRWLTPT--PEGVSAF-----IHRQTLYLSDDLHSRNMTLN 183
+L+L + + ++ R + ++ P G++A ++ +S LN
Sbjct: 154 QRLNLTIPQAFMS---NRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLN 210

Query: 184 GSGALGLGDGRYLGGNWAAIWNQSEHYNNSQAWFDNLFVRQDLGNQYYLQAGRMDQRNLS 243
L +G R L N +N S+ + S+ + + +L+ R S
Sbjct: 211 LQSGLNIGAWR-LRDNTTWSYNSSDSSSGSKNKWQH--------INTWLE--RDIIPLRS 259

Query: 244 SATGGDFGFSLLPLS--RFDGLRTGTTQAYVNHEVDQNATPVMVQVTRNARIDIYRGSEL 301
T GD F G + + + A + A++ I +
Sbjct: 260 RLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYD 319

Query: 302 LGSQFLTPGMHTLDTHSLPPGSYPLALRVYEDGILRRTETQPFS-------KGGNRFSAQ 354
+ + + PG T++ S L + + E + T P+S +G R+S
Sbjct: 320 IYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYS-- 377

Query: 355 TQWFIQGGLEDTGDKASHYDGETVMAAGFQTGLRKNISLTEGISLAHE----AWYSETRL 410
G + + + GL ++ G LA + +
Sbjct: 378 ---ITAGEYRSGN---AQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNM 431

Query: 411 NSQHAV-LDGTLDLSAGILHGTDSTSGNTEQVTYNDGFSASLWRNHTESDACSGRHPQSV 469
+ A+ +D T + L G + + YN + S T R+ S
Sbjct: 432 GALGALSVDMTQ--ANSTLPDDSQHDGQSVRFLYNKSLNES----GTNIQLVGYRYSTSG 485

Query: 470 HASMTCQTSMNASLSVPVGNWYALLGYSTSRTEGRPVYRGYDDNSDKENVF--------- 520
+ + T N Y + Y+ +K
Sbjct: 486 YFNFADTTYSRM-------NGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLG 538

Query: 521 -WRQAYIPASHRE-------SAQASATYSLNMAGMNINTHGGVWRTRNDGVNDDGLFMSV 572
Y+ SH+ Q A + +N + + D L ++V
Sbjct: 539 RTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNV 598

Query: 573 SVSYASQ-PPTMTGSNGYTSAGTDIHNSRNQKTQTSWNVNHVRSWQQDLYRELSVGFSGY 631
++ ++ + SA + + N + V +L + G++G
Sbjct: 599 NIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGG 658

Query: 632 NDDSWSGSLGGRMS--GRMGELSATISNSHQRNAGSASSLTAGYSSSLALSRNGLFWG 687
D + + ++ G G + S+S L G S + NG+ G
Sbjct: 659 GDGNSGSTGYATLNYRGGYGNANIGYSHS-----DDIKQLYYGVSGGVLAHANGVTLG 711


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2462PF005778240.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 824 bits (2129), Expect = 0.0
Identities = 306/872 (35%), Positives = 450/872 (51%), Gaps = 52/872 (5%)

Query: 4 KQPALLLFIAGVVHCANA-------HAYTFDASML-GDAAKGVDMSLFNQG-VQQPGTYR 54
K F+ V CA A F+ L D D+S F G PGTYR
Sbjct: 20 KHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYR 79

Query: 55 VDVMVNGKRVDTRDVVFKLEKDGQGTPFLASCLTVSQLSRYGVKTEDYPQLWKAAKPPDE 114
VD+ +N + TRDV F QG + CLT +QL+ G+ T + D
Sbjct: 80 VDIYLNNGYMATRDVTFNTGDSEQG---IVPCLTRAQLASMGLNTASVSGMNLL--ADDA 134

Query: 115 CADLT-AIPQAKAVLDINNQQLQLSIPQLALRPEFKGIAPEDIWDDGIPAFLMNYSARTT 173
C LT I A A LD+ Q+L L+IPQ + +G P ++WD GI A L+NY+
Sbjct: 135 CVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGN 194

Query: 174 QTDYKMDMERRDNSSWVQLQPGINIGAWRVRNATSWQR-----SSQLSGKWQAAYTYAER 228
++ + +++ LQ G+NIGAWR+R+ T+W SS KWQ T+ ER
Sbjct: 195 SVQNRIG--GNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLER 252

Query: 229 GLYSLKSRLTLGQKTSQGEIFDSVPFTGVMLASDDNMVPYSERQFAPVVRGIARTQARVE 288
+ L+SRLTLG +QG+IFD + F G LASDDNM+P S+R FAPV+ GIAR A+V
Sbjct: 253 DIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVT 312

Query: 289 VKQNGYTIYNTTVAPGPFALRDLSVTDSSGDLHVTVWEADGSTQMFVVPYQTPAIALHQG 348
+KQNGY IYN+TV PGPF + D+ +SGDL VT+ EADGSTQ+F VPY + + +G
Sbjct: 313 IKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREG 372

Query: 349 YLKYSLLAGRYRSSDSATDKAQIAQATLMYGLPWNLTAYGGIQSATHYQAASLGLGASLG 408
+ +YS+ AG YRS ++ +K + Q+TL++GLP T YGG Q A Y+A + G+G ++G
Sbjct: 373 HTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMG 432

Query: 409 RWGSLSVDGSDTHSQRQGEAVQQGASWRLRYSNQLTATGTNFSLTRWQYASQGYNTLSDV 468
G+LSVD + +S ++ G S R Y+ L +GTN L ++Y++ GY +D
Sbjct: 433 ALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADT 492

Query: 469 LDSYRHDGNRL-------------WSWRENLQPSSRTILMLSQSWGRHLGNLSLTGSRTD 515
S + N + + L ++Q GR L L+GS
Sbjct: 493 TYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGR-TSTLYLSGSHQT 551

Query: 516 WRNRPGHDDSYGLSWGTSIGGGSLSLNWNQNRTLWRNGAHSKENITSLWFSMSLSRWTGN 575
+ D+ + T+ + +L+++ + W+ G ++ + +L ++ S W +
Sbjct: 552 YWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKG---RDQMLALNVNIPFSHWLRS 608

Query: 576 -------NVSASWQMTSPSHGGQMQQVGGNGEAFSQ-QLDWEVRQSYRADAPPGGGNNSA 627
+ SAS+ M+ +G G G L + V+ Y G+
Sbjct: 609 DSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGY 668

Query: 628 LHLAWNGGYGLLGGDYSYSRAMRQMGVNIAGGIVIHHHGVTLGQPLQGSVALVEAPGASG 687
L + GGYG YS+S ++Q+ ++GG++ H +GVTLGQPL +V LV+APGA
Sbjct: 669 ATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKD 728

Query: 688 VPVGGWPGVKTDFRGDTTVGNLNVYQENTVSLDPSRLPDDAEVTQTDVRVVPTEGAVVEA 747
V GV+TD+RG + Y+EN V+LD + L D+ ++ VVPT GA+V A
Sbjct: 729 AKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRA 788

Query: 748 KFHTRIGARALMTLKREDGSAIPFGAQVTVNGQDGSAALVDTDSQVYLTGLADKGELTVK 807
+F R+G + LMTL + +PFGA VT + S+ +V + QVYL+G+ G++ VK
Sbjct: 789 EFKARVGIKLLMTLTH-NNKPLPFGAMVT-SESSQSSGIVADNGQVYLSGMPLAGKVQVK 846

Query: 808 WGA---QQCRVNYQLPAHKGIAGLYQMSGLCR 836
WG C NYQLP L Q+S CR
Sbjct: 847 WGEEENAHCVANYQLPPESQQQLLTQLSAECR 878


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2469BACINVASINB280.008 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 27.8 bits (61), Expect = 0.008
Identities = 15/56 (26%), Positives = 26/56 (46%)

Query: 1 MRIIKGFDSLLSEVKTLPDVGWLYVDKEFNLKSKMDILNKDYYLAENRDESFDMAE 56
+++ K F + L E + D+ + K KS D K A+N+ +S D A+
Sbjct: 123 IQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAATKKLTQAQNKLQSLDPAD 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2482OMPADOMAIN702e-15 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 70.3 bits (172), Expect = 2e-15
Identities = 36/128 (28%), Positives = 58/128 (45%), Gaps = 16/128 (12%)

Query: 317 QHSRVVFRGDAMFVPGQKTVSDAIRPVINKAAREIARVG---GAVTVTGHTDSQPIHSAE 373
Q + D +F + T+ + +++ +++ + G+V V G+TD I S
Sbjct: 211 QTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDR--IGSDA 268

Query: 374 FPSNLVLSEKRAAEVAALLTSGGVPAGRVHIVGKGDTVPVADN---------GSKAGRAK 424
+ N LSE+RA V L S G+PA ++ G G++ PV N A
Sbjct: 269 Y--NQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAP 326

Query: 425 NRRVEILV 432
+RRVEI V
Sbjct: 327 DRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2496HTHFIS340.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 0.002
Identities = 41/202 (20%), Positives = 65/202 (32%), Gaps = 35/202 (17%)

Query: 512 LVEPDADDKTTLQQAETALREWQGDAPVV----FPEVSAAVVA--AIVADWTGIP--AGR 563
+V PD + L + +++ + D PV+ A+ A D+ P
Sbjct: 55 VVMPDENAFDLLPR----IKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTE 110

Query: 564 MVKDEASQVLELPARLAQRVTGQDGALAQIGE--RIQTAR---AGLGDPRKPVGVFMLAG 618
++ + E R ++ + +G +Q A L + M+ G
Sbjct: 111 LIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTL---MITG 167

Query: 619 PSGVGKTETALALAEAIYGGEQNLITINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEA 678
SG GK A AL + + INM+ S L G E G T A
Sbjct: 168 ESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGH--------EKGAFTGA 219

Query: 679 VRRHPWSV-------VLLDEIE 693
R + LDEI
Sbjct: 220 QTRSTGRFEQAEGGTLFLDEIG 241


41SPA2530SPA2652Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2530-125-3.170896hypothetical protein
SPA2531018-1.060933hypothetical protein
SPA2532221-0.43719750S ribosomal protein L19
SPA2533016-0.137961tRNA(guanine-N1)methyltransferase
SPA2534117-1.44580016S rRNA processing protein
SPA2535015-1.15263630S ribosomal protein S16
SPA2536014-1.482768signal recognition particle protein
SPA2537113-2.793361hypothetical protein
SPA2538012-1.729617hypothetical protein
SPA2539213-2.156793hypothetical protein
SPA2540111-0.849646heat shock protein GrpE (heat shock protein
SPA2541012-0.947201hypothetical protein
SPA2542011-0.896544inorganic polyphosphate/ATP-NAD kinase
SPA25432153.961024DNA repair protein
SPA25443154.242373small protein A
SPA25452144.265277hypothetical protein
SPA25462144.074417hypothetical protein
SPA25472143.901590SsrA (tmRNA)-binding protein
SPA25482153.991785hypothetical protein
SPA2549-2111.247792type I secretion protein
SPA2550-1130.426645type I secretion protein, ATP-binding protein
SPA2551323-1.722722type I secretion protein
SPA2554630-2.133362hypothetical protein
SPA2555528-1.186818positive regulator of late gene transcription
SPA2556425-0.564276regulator of late gene expression
SPA25575220.439355phage tail protein
SPA25584230.549896hypothetical protein
SPA2559321-0.513744hypothetical protein
SPA2560219-1.267913phage tail protein
SPA2561320-1.763411major tail tube protein
SPA2562219-1.814773major tail sheath protein
SPA2563323-1.835154inversion of adjacent DNA; at locus of e14
SPA2564323-1.416942invasion-associated secreted protein
SPA25655241.479122phage tail fiber protein
SPA25665252.731318phage tail fiber protein
SPA25678294.933363phage tail protein
SPA25688326.009560phage baseplate assembly protein
SPA25697295.600157phage baseplate assembly protein
SPA25707306.022824phage baseplate assembly protein
SPA25718275.245128phage tail completion protein
SPA25727274.982620phage tail protein
SPA25738295.757903regulatory protein
SPA25748286.176317hypothetical protein
SPA25757295.101075lysozyme
SPA25767314.890808secretion protein
SPA25776314.399266phage tail protein
SPA25784303.537636capsid completion protein
SPA25793250.954852phage terminase
SPA2580320-2.860226major capsid protein
SPA2581321-6.213649capsid protein
SPA2582325-7.802358terminase subunit
SPA2583638-11.932904capsid portal protein
SPA2584949-15.213678hypothetical protein
SPA2585427-6.347974hypothetical protein
SPA2586323-4.096726hypothetical protein
SPA2587322-1.525460hypothetical protein
SPA2588321-1.051356hypothetical protein
SPA2589321-0.695800hypothetical protein
SPA2590320-1.052130hypothetical protein
SPA2591436-5.300451DNA adenine methylase
SPA2592533-3.076753exonuclease
SPA2593529-1.542217hypothetical protein
SPA2594631-4.726314hypothetical protein
SPA2595330-4.868389hypothetical protein
SPA2596431-5.319762hypothetical protein
SPA2597424-2.069364phage regulatory protein
SPA2598522-1.961561phage regulatory protein
SPA2599523-0.748639phage repressor protein cI
SPA2600422-0.648400phage integrase
SPA26013230.610766bacteriophage integrase
SPA26024282.328826hypothetical protein
SPA26034250.256128hypothetical protein
SPA2604322-1.206722hypothetical protein
SPA2605324-2.872577hypothetical protein
SPA2607424-1.642108hypothetical protein
SPA2608325-2.653487hypothetical protein
SPA2609424-2.382075hypothetical protein
SPA2610322-1.399850hypothetical protein
SPA26113240.293158hypothetical protein
SPA26123241.770077hypothetical protein
SPA26133251.698883hypothetical protein
SPA26144241.922086hypothetical protein
SPA26153210.124531hypothetical protein
SPA2616321-0.784198hypothetical protein
SPA2617320-1.369399hypothetical protein
SPA2618219-1.266181hypothetical protein
SPA2619119-1.759604hypothetical protein
SPA2620121-1.883770hypothetical protein
SPA2621024-3.078197hypothetical protein
SPA2622226-2.786430hypothetical protein
SPA2623122-1.578523hypothetical protein
SPA26240151.259690hypothetical protein
SPA26250132.769937hypothetical protein
SPA2626-1132.655281Flagellar synthesis: repressor of fliC
SPA26270122.402791Flagellar synthesis: phase 2 flagellin (filament
SPA26290142.915276glycosyltransferase
SPA26300151.526351ABC transporter
SPA2631219-2.208390ferric enterochelin esterase
SPA2632325-5.813447hypothetical protein
SPA2633326-6.252189TonB-dependent outer membrane siderophore
SPA2635126-5.391530inner membrane protein
SPA2636121-4.423453hypothetical protein
SPA2637016-3.659379virulence protein
SPA2638-213-1.383683transcriptional regulator
SPA2639-2141.426805nickel transporter
SPA2640-3142.955881two-component system sensor kinase
SPA2641-2162.593089transcriptional regulator
SPA2642-1203.390576hypothetical protein
SPA26430223.916063hypothetical protein
SPA26440203.949501hypothetical protein
SPA26451172.890723hypothetical protein
SPA26462172.831275Gab protein
SPA26473152.136687GAB DTP gene cluster repressor
SPA26484140.931727succinate-semialdehyde dehydrogenase
SPA2649414-0.4628914-aminobutyrate aminotransferase
SPA2650318-1.669983GabA permease (4-amino butyrate transport
SPA2651123-1.535078transcriptional regulator
SPA2652323-4.239660LysM domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2543RTXTOXIND310.009 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.009
Identities = 31/198 (15%), Positives = 64/198 (32%), Gaps = 36/198 (18%)

Query: 177 QQQSQERAARAELLQYQLKELNDFNPQAGEFEQIDEEYKRLANSGQLLTTSQNALALLAD 236
+ QS AR E +YQ+ + + E + DE Y + + ++L + +
Sbjct: 138 KTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFS- 196

Query: 237 GEDVNLQSQLYSAKQLVSELVGMDSKLSGILDMLEEATIQLTEASDELRHYCERLDLDPN 296
Q+Q Y Q L ++ +L A I E +
Sbjct: 197 ----TWQNQKY---QKELNLDKKRAERLTVL-----ARINRYENLSRV------------ 232

Query: 297 RLFELEQRIAKQISLARKHHVSPEALPQLYQSLLEEQQQLDDQADSLETLTLAVNKHHQQ 356
+ R+ SL K ++ ++LE++ + + + L + + +
Sbjct: 233 ----EKSRLDDFSSLLHKQAIA-------KHAVLEQENKYVEAVNELRVYKSQLEQIESE 281

Query: 357 ALETAQALHQQRQFYAQE 374
L + Q + E
Sbjct: 282 ILSAKEEYQLVTQLFKNE 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2546FLGMOTORFLIM280.018 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 27.6 bits (61), Expect = 0.018
Identities = 16/78 (20%), Positives = 30/78 (38%), Gaps = 8/78 (10%)

Query: 36 GSRVLESSPAQMTAAVDVSKAGISKTFTTRNQLTRNQSILMHLVDGPFKKLIGGWK---- 91
G+ VLE P+ + +D G + + LT I +++G +++ +
Sbjct: 113 GNAVLEVDPSITFSIIDRLFGGTGQAAKVQRDLT---DIENSVMEGVIVRILANVRESWT 169

Query: 92 -FTPLSPEACRIEFQLDF 108
L P +IE F
Sbjct: 170 QVIDLRPRLGQIETNPQF 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2548INTIMIN463e-06 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 46.2 bits (109), Expect = 3e-06
Identities = 63/315 (20%), Positives = 107/315 (33%), Gaps = 38/315 (12%)

Query: 2724 TPAQTNGQPLLAFAQDKAGNTGIAAGFTAPDTRVPEAPIITNVVDDVGIYTGAIANGQ-- 2781
+N + A A D+ GN+ T + V D T A A+G
Sbjct: 518 VQGGSNVYKVTARAYDRNGNSSNNVLLTITVLSNGQVVDQVGVTDFTADKTSAKADGTEA 577

Query: 2782 VTNDAQPTLNGTAQAGATVS--IYNNGALLGTTTANASGNWSFTPTGNLTEGSHAFT-AT 2838
+T A NG AQA VS I + A+L +AN +G+ T T L +
Sbjct: 578 ITYTATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVT--LKSDKPGQVVVS 635

Query: 2839 ATNANGTGSVSSAATVIVDTLAPGTPSGTLSADGGSLSGLAEANSTVTVTLT-------- 2890
A A T ++++ A + VD + AD + +A +T T+
Sbjct: 636 AKTAEMTSALNANAVIFVDQTK--ASITEIKAD--KTTAVANGQDAITYTVKVMKGDKPV 691

Query: 2891 -----------GGVTLTT-TAGSNGAWSLTLPTKQIEGQLINVTATDAAGN-ASGTLGIT 2937
G ++ +T +NG +TL + L++ +D A + + +
Sbjct: 692 SNQEVTFTTTLGKLSNSTEKTDTNGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFF 751

Query: 2938 APVLPLAARDNITSLDLTSTAVTSTQNYSDYGLLLVGALGNVASVLGN------DTTQVE 2991
+ I + T Y L G G N D + +
Sbjct: 752 TTLTIDDGNIEIVGTGVKGKLPTVWLQYGQVNLKASGGNGKYTWRSANPAIASVDASSGQ 811

Query: 2992 FTIAEGGTGDVTIDA 3006
T+ E GT +++ +
Sbjct: 812 VTLKEKGTTTISVIS 826



Score = 41.6 bits (97), Expect = 7e-05
Identities = 64/272 (23%), Positives = 91/272 (33%), Gaps = 22/272 (8%)

Query: 1508 TLPVTSALPDGVYTLTAIAADAAGNSSGVSNSFTFTVDTVPLQPPVVN--EILDDVAPVT 1565
LP VY +TA A D GNS SN+ T+ TV VV+ + D A T
Sbjct: 513 ILPAYVQGGSNVYKVTARAYDRNGNS---SNNVLLTI-TVLSNGQVVDQVGVTDFTADKT 568

Query: 1566 GPLTDG--AFTNDRTLTINGSGENGSTVTIYDNGVAIGTALVTDGVWTFN-----TSELS 1618
DG A T T+ NG + V+ + GTA+++ N T L
Sbjct: 569 SAKADGTEAITYTATVKKNGVAQANVPVSF---NIVSGTAVLSANSANTNGSGKATVTLK 625

Query: 1619 EASHALTFSATDDAGNTTAQTQPITITVDITAPPAPTIQTVADDGTRVAGLADPYA-TVE 1677
+ A T+A I VD T I+ AD T VA D TV+
Sbjct: 626 SDKPGQVVVSAKTAEMTSALNANAVIFVDQTKASITEIK--ADKTTAVANGQDAITYTVK 683

Query: 1678 IHHADGTLVGSAVANGTGEFVVTLSPAQTDGGTLTAIAIDRAGNNGPATNFPASDSGLPA 1737
+ D + V T ++ S +TD + + + G +
Sbjct: 684 VMKGDKPVSNQEVTFTTTLGKLSNSTEKTDTNGYAKVTLT-STTPGKSLVSARVSDVAVD 742

Query: 1738 VPAITAIEDDVGSIQGNIAA--GGATDDTMPT 1767
V A +I G +PT
Sbjct: 743 VKAPEVEFFTTLTIDDGNIEIVGTGVKGKLPT 774



Score = 37.4 bits (86), Expect = 0.001
Identities = 75/370 (20%), Positives = 137/370 (37%), Gaps = 45/370 (12%)

Query: 2197 IYNGSALVGTA-QVQANGSWSFT-------PSTSLGAGVWNLTATATDAAGNTSAASEIR 2248
+++ SAL Q+Q +GS S G+ V+ +TA A D GN+S + +
Sbjct: 486 VWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNVYKVTARAYDRNGNSS-NNVLL 544

Query: 2249 SFTIDTTAPAAPVIDTVYDGTGPITGNLSSGQ--ITDEARPVISGTREAN--TAIRLYDN 2304
+ T+ + V D T T + G IT A +G +AN + +
Sbjct: 545 TITV-LSNGQVVDQVGVTDFTADKTSAKADGTEAITYTATVKKNGVAQANVPVSFNIVSG 603

Query: 2305 GTLLAEIPADNSSSWRYTPDASLATGNHVITVIAVDAAGNASPV-SDSVNFVVDTTPPLT 2363
+L+ A+ + S + T +L + V++ A S + +++V FV T +T
Sbjct: 604 TAVLSANSANTNGSGKAT--VTLKSDKPGQVVVSAKTAEMTSALNANAVIFVDQTKASIT 661

Query: 2364 PVITSVSDDQAPGLGTIANGQN--TNDPTPTFSGTAEAGATITLYENGTVIGTTTAQ--P 2419
+ A +ANGQ+ T + +T + +T +
Sbjct: 662 EI--KADKTTA-----VANGQDAITYTVKVMKGDKPVSNQEVTFTTTLGKLSNSTEKTDT 714

Query: 2420 DGAWSVSTSTLASGTHVITAVATDAAGNSSPNSTAFTLTVDTTAPQTPILTSVVDDVAGG 2479
+G V+ ++ G +++A +D A + F I ++ V G
Sbjct: 715 NGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFF-------TTLTIDDGNIEIVGTG 767

Query: 2480 VTGNLANGQITNDNRPTLNGTAEAGSV-VTIYDGNTLLGVTSANAGGAWSFTPTTGLNDG 2538
V G L + +N A G+ T N + A++G T G
Sbjct: 768 VKGKLPTVWL---QYGQVNLKASGGNGKYTWRSANPAIASVDASSG------QVTLKEKG 818

Query: 2539 TRILTVTATD 2548
T ++V ++D
Sbjct: 819 TTTISVISSD 828


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2549RTXTOXIND364e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 35.6 bits (82), Expect = 4e-04
Identities = 32/224 (14%), Positives = 63/224 (28%), Gaps = 32/224 (14%)

Query: 209 DVVQTEARIESARSQLAQYQANLDSAKASLMSWLGWNSLNGINNDFPAKLARSCETATPD 268
+ EA +S L Q + + S D P S E
Sbjct: 128 TALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRL 187

Query: 269 DRLVPAVLAAW-AQANVARANLDYASAQ---MTPTISLEPSVQHYLNDKYPSHEVLDKTQ 324
L+ + W Q NLD A+ + I+ ++ + L Q
Sbjct: 188 TSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQ 247

Query: 325 YSTWVKVEMPLYQGGGLTARRNAASHAVDAAQSTIQRTRLDVRQKLMEARSQAMSLASAL 384
V + ++ +L +SQ + S
Sbjct: 248 AIAKHAVL-------------------------EQENKYVEAVNELRVYKSQLEQIES-- 280

Query: 385 QILRRQQQLSERTRELYQQQYLDLGSRPLLDVLNAEQEVYQARF 428
+IL +++ T +L++ + LD + ++ E+ +
Sbjct: 281 EILSAKEEYQLVT-QLFKNEILDKLRQTTDNIGLLTLELAKNEE 323


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2551RTXTOXIND2433e-78 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 243 bits (621), Expect = 3e-78
Identities = 95/432 (21%), Positives = 176/432 (40%), Gaps = 56/432 (12%)

Query: 9 ERAFSGAGRIVLICSLLFLILGI-WAWFGRLDEVSTGNGKVIPSSREQVLQSLDGGILAQ 67
E S R+V + FL++ + G+++ V+T NGK+ S R + ++ ++ I+ +
Sbjct: 50 ETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKE 109

Query: 68 LTVREGDRVQANQIVARLDPTRLASNVGESAAKYRASLASSARLTAEVSDLPL------- 120
+ V+EG+ V+ ++ +L ++ ++ + + R + L
Sbjct: 110 IIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELK 169

Query: 121 --AFPAELNGWPDLIAAETRLYKSR-----------RAQLADTEAELRDALASVNK---- 163
P N + + T L K + L AE LA +N+
Sbjct: 170 LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENL 229

Query: 164 ------ELTITQRLEKSGAASHVEVLRLQRQKSDLG---------------------LKI 196
L L A + VL + + + +
Sbjct: 230 SRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEY 289

Query: 197 TDLRSQYYVQAREALSKANAEVDMLSAILKGREDSVTRLTVRSPVRGIVKNIQVTTIGGV 256
+ + + + L + + +L+ L E+ +R+PV V+ ++V T GGV
Sbjct: 290 QLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGV 349

Query: 257 IPPNGEMMEIVPVDDRLLIETRLSPRDIAFIHPGQRALVKITAYDYAIYGGLDGVVETIS 316
+ +M IVP DD L + + +DI FI+ GQ A++K+ A+ Y YG L G V+ I+
Sbjct: 350 VTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNIN 409

Query: 317 PDTIQDKVKPEIFYYRVFIRTHQDYLQNKSGRRFSIVPGMIATVDIKTGEKTIVDYLIKP 376
D I+D+ + V I ++ L + + GM T +IKTG ++++ YL+ P
Sbjct: 410 LDAIEDQRLGL--VFNVIISIEENCLSTG-NKNIPLSSGMAVTAEIKTGMRSVISYLLSP 466

Query: 377 F-NRAKEALRER 387
E+LRER
Sbjct: 467 LEESVTESLRER 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2558IGASERPTASE350.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.7 bits (79), Expect = 0.002
Identities = 20/98 (20%), Positives = 32/98 (32%), Gaps = 6/98 (6%)

Query: 88 ESPTKKQTQALEAQWRAVSRLEQKQQQETRQMAAARAELYRLGLSAGGGARETARIARET 147
E+P A ++ KQ+ +T + A RE A+ A+
Sbjct: 1022 EAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDAT------ETTAQNREVAKEAKSN 1075

Query: 148 ERYNRQLAEQERRLREVGERQRKLNAIKAKAEKTRELR 185
+ N Q E + E E Q A EK + +
Sbjct: 1076 VKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAK 1113


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2564SOPEPROTEIN432e-158 Salmonella type III secretion SopE effector protein ...
		>SOPEPROTEIN#Salmonella type III secretion SopE effector protein

signature.
Length = 239

Score = 432 bits (1112), Expect = e-158
Identities = 237/239 (99%), Positives = 237/239 (99%)

Query: 2 TKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTES 61
TKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTES
Sbjct: 1 TKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTES 60

Query: 62 SATHFHRGSASEGRAVLTNKVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSK 121
SATHFHRGSASEGRAVLTNKVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSK
Sbjct: 61 SATHFHRGSASEGRAVLTNKVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSK 120

Query: 122 NKDQCCNLLISKGINIAPFLQEIGEAAKNVGLPGTTKNDVFTPSGAGANPFITPLISSAN 181
NKDQCCNLLISKGINIAPFLQEIGEAAKN GLPGTTKNDVFTPSGAGANPFITPLISSAN
Sbjct: 121 NKDQCCNLLISKGINIAPFLQEIGEAAKNAGLPGTTKNDVFTPSGAGANPFITPLISSAN 180

Query: 182 SKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQYTP 240
SKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQ TP
Sbjct: 181 SKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQNTP 239


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA257660KDINNERMP250.035 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 24.9 bits (54), Expect = 0.035
Identities = 11/32 (34%), Positives = 16/32 (50%)

Query: 15 AVLLAWLGDLSLKDASTVGGVLIGVLMLAINW 46
A W+ DLS +D + +L+GV M I
Sbjct: 449 APFALWIHDLSAQDPYYILPILMGVTMFFIQK 480


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2602ICENUCLEATIN300.033 Ice nucleation protein signature.
		>ICENUCLEATIN#Ice nucleation protein signature.

Length = 1258

Score = 30.1 bits (67), Expect = 0.033
Identities = 21/70 (30%), Positives = 33/70 (47%)

Query: 425 ATLMAGAIQQVSAGDFSQAVKGNRLASITGNEETEIAGQLSTKVAGAMNVDVGGTLTEKI 484
++L AG A S + G ITGN IAG+ S++ AG + + G + ++
Sbjct: 1070 SSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGADSVQM 1129

Query: 485 AALRKSVAAG 494
A R + AG
Sbjct: 1130 AGERGKLIAG 1139



Score = 30.1 bits (67), Expect = 0.034
Identities = 20/58 (34%), Positives = 29/58 (50%)

Query: 437 AGDFSQAVKGNRLASITGNEETEIAGQLSTKVAGAMNVDVGGTLTEKIAALRKSVAAG 494
AG S + GNR I G ++ AG ST ++GA +V + G + IA + AG
Sbjct: 1090 AGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGADSVQMAGERGKLIAGADSTQTAG 1147



Score = 29.3 bits (65), Expect = 0.045
Identities = 21/70 (30%), Positives = 33/70 (47%)

Query: 425 ATLMAGAIQQVSAGDFSQAVKGNRLASITGNEETEIAGQLSTKVAGAMNVDVGGTLTEKI 484
+TLMAG +A + S G S+ G + + IAG ST+ AG + G + +
Sbjct: 942 STLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQT 1001

Query: 485 AALRKSVAAG 494
A ++ AG
Sbjct: 1002 AEHSSTLTAG 1011


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2627FLAGELLIN2742e-88 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 274 bits (702), Expect = 2e-88
Identities = 262/515 (50%), Positives = 310/515 (60%), Gaps = 18/515 (3%)

Query: 2 AQVINTNSLSLLTQNNLNKSQSALGTAIERLSSGLRINSAKDDAAGQAIANRFTANIKGL 61
AQVINTNSLSLLTQNNLNKSQS+L +AIERLSSGLRINSAKDDAAGQAIANRFT+NIKGL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 TQASRNANDGISIAQTTEGALNEINNNLQRVRELAVQSANSTNSQSDLDSIQAEITQRLN 121
TQASRNANDGISIAQTTEGALNEINNNLQRVREL+VQ+ N TNS SDL SIQ EI QRL
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EIDRVSGQTQFNGVKVLAQDNTLTIQVGANDGETIDIDLKQINSQTLGLDSLNVQKAYDV 181
EIDRVS QTQFNGVKVL+QDN + IQVGANDGETI IDL++I+ ++LGLD NV +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEA 180

Query: 182 KDTAVTTKAYADNGTTLDASGLDDAAIKAAIGGTTGTAAVTSGTVKFDADNNKYFVTIGG 241
+ + G D + + + T+ TV D G
Sbjct: 181 TVGDLKSSFKNVTGY--DTYAVGANKYRVDVNSGAVVTDTTAPTV---PDKVYVNAANGQ 235

Query: 242 FTGADAAKNGDYEVNVATDGKVTLATSATKTTMPAGAATKTEVQELKDTPAVVSADAKNA 301
T DA N + + A +A + E + D K
Sbjct: 236 LTTDDAENNTAVD---LFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTG 292

Query: 302 LIAGGVDTADANAATLVKMSYTDKNGKTIEGGYALKAGDKYYAA------DYDEATGAIK 355
G + N + G L++ Y + +D+ T
Sbjct: 293 NDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNES 352

Query: 356 AKTTSYTAADGTTKTAANQLGGVDG----KTEVVTIDGKTYNASKAAGHDFKAQPELAEA 411
AK + A + + + G + + VT+ GKT K A E A A
Sbjct: 353 AKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAA 412

Query: 412 AAKTTENPLQKIDAALAQVDALRSDLGAVQNRFNSAITNLGNTVNNLSEARSRIEDSDYA 471
A K+T NPL ID+AL++VDA+RS LGA+QNRF+SAITNLGNTV NL+ ARSRIED+DYA
Sbjct: 413 AKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYA 472

Query: 472 TEVSNMSRAQILQQAGTSVLAQANQVPQNVLSLLR 506
TEVSNMS+AQILQQAGTSVLAQANQVPQNVLSLLR
Sbjct: 473 TEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLLR 507


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2630PF05272340.007 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 33.5 bits (76), Expect = 0.007
Identities = 47/217 (21%), Positives = 66/217 (30%), Gaps = 49/217 (22%)

Query: 992 PPG----TVVAVVGRSGTGKSTLIKLLAGLYSPGSGQIRVGER-----------LIDAAS 1036
PG V + G G GKSTLI L GL +G + +
Sbjct: 590 EPGCKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSE 649

Query: 1037 LSDYRRQTGLVTQDVALFSGDIAENI-RYSRPDSSDTEVEIAARQAGLFETV---QHL-- 1090
++ +RR D + RY V+ RQ ++ T Q+L
Sbjct: 650 MTAFRR------ADAEAVKAFFSSRKDRYRGA--YGRYVQDHPRQVVIWCTTNKRQYLFD 701

Query: 1091 PLGFRT--PVNNGG----TDLSAGQRQLIALA--------RAHLA--QAHILLLDEATAR 1134
G R PV G L + QL A A R + I E R
Sbjct: 702 ITGNRRFWPVLVPGRANLVWLQKFRGQLFAEALHLYLAGERYFPSPEDEEIYFRPEQELR 761

Query: 1135 -IDRSAEERLMTSLTRVTHTEKRIALIVAHRLTTARR 1170
++ + RL LTR A A + +
Sbjct: 762 LVETGVQGRLWALLTREG---APAAEGAAQKGYSVNT 795


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2640PF06580330.003 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.003
Identities = 23/101 (22%), Positives = 38/101 (37%), Gaps = 21/101 (20%)

Query: 370 LLDNALKY----TPEQGIVTARLEQDGDAVTLVVEDSGPGIDDEHIHLALQPFHRLDNVG 425
L++N +K+ P+ G + + +D VTL VE++G L N
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLA--------------LKNTK 308

Query: 426 NVAGAGIGLALVND-IARLHRTHPHFSRSEALGGLYVRIRF 465
G GL V + + L+ T SE G + +
Sbjct: 309 E--STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2641HTHFIS972e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 96.8 bits (241), Expect = 2e-25
Identities = 35/122 (28%), Positives = 61/122 (50%), Gaps = 1/122 (0%)

Query: 2 RLLLAEDNRELAHWLEKALVQNGFAVDCVFDGLAADHLLHSEMYALAVLDINMPGMDGLE 61
+L+A+D+ + L +AL + G+ V + + + L V D+ MP + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VVQRLRKRGQTLPVLLLTARSAVADRVKGLNVGADDYLPKPFELEE-LDARLRALLRRSA 120
++ R++K LPVL+++A++ +K GA DYLPKPF+L E + RAL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 121 GQ 122

Sbjct: 125 RP 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2652INTIMIN270.028 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 27.3 bits (60), Expect = 0.028
Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 6/69 (8%)

Query: 92 SVDDQVKTTTPAAESQFYTVKSGDTLSAISKQVYGNANLYNKIFEANKPMLKSPE---KI 148
D ++ T FYT+K+G+T++ +SK N + I+ NK + S K
Sbjct: 48 GSDSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLST---IWSLNKHLYSSESEMMKA 104

Query: 149 YPGQVLRIP 157
PGQ + +P
Sbjct: 105 EPGQQIILP 113


42SPA2692SPA2784Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2692-1153.076927PTS system glucitol/sorbitol-specific
SPA2693-1142.290304sorbitol-6-phosphate 2-dehydrogenase
SPA2694-1142.516556glucitol operon activator protein
SPA2695-1133.805192glucitol operon repressor
SPA26960134.473163phosphosugar binding protein
SPA2697-1124.046387sigma-54-dependent transriptional regulator
SPA26980123.423734flavoprotein
SPA26990134.236099rubredoxin reductase
SPA27001144.692769hydrogenase maturation protein
SPA2701-1192.923085electron transport protein
SPA2702-1253.560610hypothetical protein
SPA27031304.501530hydrogenase 3 maturation protease
SPA27041305.540978formate hydrogenlyase maturation protein
SPA27051285.593809formate hydrogenlyase subunit 7
SPA27062274.974982formate hydrogenlyase subunit 6
SPA27072244.559082formate hydrogenlyase subunit 5
SPA27082184.515601formate hydrogenlyase subunit 4
SPA27092184.260800formate hydrogenlyase subunit 3
SPA27101172.725195formate hydrogenlyase subunit 2
SPA27111153.146409formate hydrogenlyase regulatory protein
SPA27120132.974568HypA protein
SPA27130143.358960hydrogenase isoenzymes formation protein HypB
SPA27140142.736376hydrogenase isoenzymes formation protein HypC
SPA2715-1152.544807hydrogenase expression/formation protein HypD
SPA27160151.942838hydrogenase isoenzymes formation protein HypE
SPA2717-1130.604166transcriptional activator of the formate
SPA2718-213-0.703291hypothetical protein
SPA2719-213-2.162286Iron transport protein, periplasmic-binding
SPA2720-220-5.710848Iron transport protein, ATP-binding component
SPA2721-126-7.333092Iron transport protein, inner membrane
SPA2722134-9.527566Iron transport protein, inner membrane
SPA2723342-10.388886hypothetical protein
SPA2724240-9.607902AraC-family transcriptional regulator
SPA2725340-9.528723AraC-family transcriptional regulator
SPA2726338-7.246929hypothetical protein found within S. typhi
SPA2727335-6.144703oxygen-regulated invasion protein
SPA2728334-7.166271oxygen-regulated invasion protein
SPA2729331-8.373296pathogenicity 1 island effector protein
SPA2730330-8.973543pathogenicity 1 island effector protein
SPA2731231-9.358551pathogenicity 1 island effector protein
SPA2732231-9.703657pathogenicity 1 island effector protein
SPA2733232-11.174086AraC family transcriptional regulator
SPA2734233-11.145994invasion protein regulator
SPA2735232-9.182888cell invasion protein
SPA2736232-8.349909stpA-like protein
SPA2737028-7.287448chaperone (associated with virulence)
SPA2738125-5.217684hypothetical protein
SPA2739125-5.263052acyl carrier protein
SPA2740123-5.646466pathogenicity island 1 effector protein
SPA2741121-5.457526pathogenicity island 1 effector protein
SPA2742122-5.332648pathogenicity island 1 effector protein
SPA2743122-6.050004pathogenicity island 1 effector protein
SPA2744-127-7.033173hypothetical protein
SPA2745-127-6.157893secretory protein (associated with virulence)
SPA2746-127-5.499219secretory protein (associated with virulence)
SPA2747-223-3.938679secretory protein (associated with virulence)
SPA2748-224-4.247423secretory protein (associated with virulence)
SPA2749-224-4.594316surface presentation of antigens protein
SPA2750-123-5.714108antigen presentation protein SpaN
SPA2751-224-6.199650virulence-associated secretory protein
SPA2752-224-6.056000virulence-associated secretory apparatus ATP
SPA2753-127-7.845117virulence-associated secretory protein
SPA2754-125-7.472499secretory protein
SPA2755-127-7.222594cell invasion protein
SPA2756128-6.932021secretory protein (associated with virulence)
SPA2757430-7.068579AraC-family regulatory protein
SPA2758534-7.774973cell adherance/invasion protein
SPA2759-313-0.209314hypothetical protein
SPA2760-3120.099346hypothetical protein
SPA2761-3110.672621hypothetical protein
SPA2764-2101.431805hypothetical protein
SPA2765-3102.656865hypothetical protein
SPA2766-2103.253207DNA mismatch repair protein
SPA2767-2113.232898hypothetical protein
SPA2768-2113.125568membrane transport protein
SPA2769-3124.152563LysR-family transcriptional regulator
SPA2770-2134.937533permease
SPA2771-2124.401390hypothetical protein
SPA2772-2134.452018hydroxypyruvate isomerase
SPA2773-1113.732914sugar aldolase
SPA2774-1113.635396hypothetical protein
SPA27751112.1556773-hydroxyisobutyrate dehydrogenase
SPA27760150.705208DeoR family transcriptional regulator
SPA2777-1140.771900slyA-like protein
SPA2778-1141.061775hypothetical protein
SPA27791170.416421hypothetical protein
SPA27800170.858698RNA polymerase sigma subunit RpoS (sigma-38)
SPA27811161.822252lipoprotein NlpD
SPA27822172.700445L-isoaspartyl protein carboxyl methyltransferase
SPA27832161.927448stationary-phase survival protein
SPA27842151.532738hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2693DHBDHDRGNASE841e-21 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 84.3 bits (208), Expect = 1e-21
Identities = 67/257 (26%), Positives = 120/257 (46%), Gaps = 7/257 (2%)

Query: 3 QVAVVIGGGQTLGAFLCRGLAEEGYRVAVVDIQSDKAANVAQEINADFGEGMAYGFGADA 62
++A + G Q +G + R LA +G +A VD +K V + A+ A F AD
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE--ARHAEAFPADV 66

Query: 63 TSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCARE 122
++ ++ ++ G +D+LV AG+ + I +++ + VN G F +R
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 123 FSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFGGVGLTQSLALDLAEYGITVHSL 182
S+ M D G I+ + S V + Y+++K V T+ L L+LAEY I + +
Sbjct: 127 VSKYM-MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 183 MLGNLLKSPMFQSL-LPQYATKLGIKPDEVEQYYIDKVPLKRGCDYQDVLNMLLFYASPK 241
G+ ++ M SL + + IK +E + +PLK+ D+ + +LF S +
Sbjct: 186 SPGS-TETDMQWSLWADENGAEQVIKGS-LETFKTG-IPLKKLAKPSDIADAVLFLVSGQ 242

Query: 242 ASYCTGQSINVTGGQVM 258
A + T ++ V GG +
Sbjct: 243 AGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2695ARGREPRESSOR270.044 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 27.1 bits (60), Expect = 0.044
Identities = 10/45 (22%), Positives = 18/45 (40%), Gaps = 5/45 (11%)

Query: 1 MKPRQRQAAILEHLQKQGKCSVEEL-----AQYFDTTGTTIRKDL 40
M QR I E + + +EL ++ T T+ +D+
Sbjct: 1 MNKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDI 45


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2697HTHFIS373e-127 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 373 bits (958), Expect = e-127
Identities = 122/340 (35%), Positives = 180/340 (52%), Gaps = 21/340 (6%)

Query: 183 MIGLSPAMTQLKKEIEIVAGSDLNVLIGGETGTGKELVAKAIHQGSPRAVNPLVYLNCAA 242
++G S AM ++ + + + +DL ++I GE+GTGKELVA+A+H R P V +N AA
Sbjct: 139 LVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAA 198

Query: 243 LPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYG 302
+P + ESELFGH KGAFTGA + +G+FE A+ GTLFLDEIG++ + Q +LLRVLQ G
Sbjct: 199 IPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQG 258

Query: 303 DIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLFVPPLRERGDDVV 362
+ VG +R DVR++AATN+DL++ + G FR DL++RL+V PL +PPLR+R +D+
Sbjct: 259 EYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIP 318

Query: 363 LLAGYFCEQCRLRLGLSRVVLSPGARRHLLNYGWPGNVRELEHAIHRAVVLARATRAGDE 422
L +F +Q + GL A + + WPGNVRELE+ + R L E
Sbjct: 319 DLVRHFVQQAE-KEGLDVKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITRE 377

Query: 423 VVL-----EEQHFALS---------------EDVLPAPSAESFLALPACRNLRESTENFQ 462
++ E + E+ + A ALP +
Sbjct: 378 IIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEME 437

Query: 463 REMIRQALAQNNHNWAASARALETDVANLHRLAKRLGLKD 502
+I AL N +A L + L + + LG+
Sbjct: 438 YPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSV 477


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2714TYPE4SSCAGA270.011 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 27.0 bits (59), Expect = 0.011
Identities = 19/75 (25%), Positives = 37/75 (49%), Gaps = 8/75 (10%)

Query: 12 IDGNQAKVD--VCGIQRDVDLTLVGSCDENGQPRLGQWVLVHVGFAMSVINEAEARDTLD 69
I GNQ + D G+ D L ++NG+P G W+ + + F + ++ ++ D +
Sbjct: 171 IIGNQIRTDQKFMGV-FDESLKERQEAEKNGEPTGGDWLDIFLSF---IFDKKQSSDVKE 226

Query: 70 ALQN--MFDVEPDVG 82
A+ + V+PD+
Sbjct: 227 AINQEPVPHVQPDIA 241


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2717HTHFIS384e-129 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 384 bits (988), Expect = e-129
Identities = 142/373 (38%), Positives = 207/373 (55%), Gaps = 39/373 (10%)

Query: 350 YQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYNVLKQVEMVAQSDSTVLILG 409
E+ + R + E +L + + ++GRS AM + + + + Q+D T++I G
Sbjct: 108 LTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITG 167

Query: 410 ETGTGKELIARAIHNLSGRSGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRF 469
E+GTGKEL+ARA+H+ R V +N AA+P L+ES+LFGHE+GAFTGA + GRF
Sbjct: 168 ESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRF 227

Query: 470 ELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLEKMV 529
E A+ +LFLDE+GDMP++ Q +LLRVLQ+ E+ +G I++DVR++AATN+DL++ +
Sbjct: 228 EQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSI 287

Query: 530 ADREFRNDLYYRLNVFPIQLPPLRERPEDIPLLVKAFTFKIARRMGRNIDSIPAETLRTL 589
FR DLYYRLNV P++LPPLR+R EDIP LV+ F + A + G ++ E L +
Sbjct: 288 NQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFV-QQAEKEGLDVKRFDQEALELM 346

Query: 590 SSMEWPGNVRELENVVERAVLLTRGNVLQLS-LPDITAVTPDTSPVATESAKEG------ 642
+ WPGNVRELEN+V R L +V+ + + SP+ +A+ G
Sbjct: 347 KAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQ 406

Query: 643 ----------------------------EDEYQLIIRVLKETNGVVAGPKGAAQRLGLKR 674
E EY LI+ L T G AA LGL R
Sbjct: 407 AVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIK---AADLLGLNR 463

Query: 675 TTLLSRMKRLGID 687
TL +++ LG+
Sbjct: 464 NTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2719adhesinb321e-112 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 321 bits (824), Expect = e-112
Identities = 89/309 (28%), Positives = 164/309 (53%), Gaps = 14/309 (4%)

Query: 4 LHRLKTLLIAGIVAILAL-------SPAYAKEKFKVITTFTVIADMAKNVAGDAAEVSSI 56
+ + + L++ + + S K V+ T ++IAD+ KN+AGD + SI
Sbjct: 1 MKKCRFLVLLLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSI 60

Query: 57 TKPGAEIHEYQPTPGDIKRAQGAQLILANGLNLER----WFARFYQHLSGVPE---VVVS 109
G + HEY+P P D+K+ A LI NG+NLE WF + ++ VS
Sbjct: 61 VPVGQDPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVENAKKKENKDYYAVS 120

Query: 110 TGVKPMGITEGPYNGKPNPHAWMSAENALIYVDNIRDALVKYDPDNAQIYKQNAERYKAK 169
GV + + GK +PHAW++ EN +IY NI L + DP N + Y++N + Y K
Sbjct: 121 EGVDVIYLEGQSEKGKEDPHAWLNLENGIIYAQNIAKRLSEKDPANKETYEKNLKAYVEK 180

Query: 170 IRQMADPLRAELEKIPADQRWLVTSEGAFSYLARDNDMKELYLWPINADQQGTPKQVRKV 229
+ + + + IP +++ +VTSEG F Y ++ ++ Y+W IN +++GTP Q++ +
Sbjct: 181 LSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTL 240

Query: 230 IDTIKKHHIPAIFSESTVSDKPARQVARESGAHYGGVLYVDSLSAADGPVPTYLDLLRVT 289
++ ++K +P++F ES+V D+P + V++++ ++ DS++ +Y +++
Sbjct: 241 VEKLRKTKVPSLFVESSVDDRPMKTVSKDTNIPIYAKIFTDSVAEKGEEGDSYYSMMKYN 300

Query: 290 TETIVNGIN 298
E I G++
Sbjct: 301 LEKIAEGLS 309


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2725BORPETOXINA310.007 Bordetella pertussis toxin A subunit signature.
		>BORPETOXINA#Bordetella pertussis toxin A subunit signature.

Length = 269

Score = 30.5 bits (68), Expect = 0.007
Identities = 16/57 (28%), Positives = 30/57 (52%), Gaps = 8/57 (14%)

Query: 201 IISDLTRKWSQAEVAGKLFMSVSSLKRKLAAEEVSFSKIYLDARMNQAIKLLRMGAG 257
++ LT + Q + F+S SS +R ++++YL+ RM +A++ R G G
Sbjct: 66 VLDHLTGRSCQVGSSNSAFVSTSSSRR--------YTEVYLEHRMQEAVEAERAGRG 114


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2729FLGMRINGFLIF437e-07 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 42.6 bits (100), Expect = 7e-07
Identities = 33/167 (19%), Positives = 63/167 (37%), Gaps = 10/167 (5%)

Query: 23 LLKGLDQEQANEVIAVLQMHNIEANKIDSGKLGYSITVAEPDFTAAVYWIKTYQLPPRPR 82
L L + ++A L NI + +G +I V + LP
Sbjct: 53 LFSNLSDQDGGAIVAQLTQMNI-PYRFANG--SGAIEVPADKVHELRLRLAQQGLPKGGA 109

Query: 83 VEIAQMFPADSLVSSPRAEKARLYSAIEQRLEQSLQTMEGVLSARVHISYDIDA---GEN 139
V + + S +E+ A+E L ++++T+ V SARVH++ + E
Sbjct: 110 VGFE-LLDQEKFGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQ 168

Query: 140 GRPPKPVHLSALAVYERGSPLAHQISDIKRFLKNSFADVDYDNISVV 186
P V ++ QIS + + ++ A + N+++V
Sbjct: 169 KSPSASVTVTLEPGRALDEG---QISAVVHLVSSAVAGLPPGNVTLV 212


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2733PF07212280.044 Hyaluronoglucosaminidase
		>PF07212#Hyaluronoglucosaminidase

Length = 336

Score = 28.1 bits (62), Expect = 0.044
Identities = 12/39 (30%), Positives = 21/39 (53%)

Query: 234 MSTSTLKRKLAEEGTSFSDIYLSARMNQAAKLLRIGNHN 272
+S +K++ +GT+ IY+++ KLLRI N
Sbjct: 241 LSIDIVKKQKGGKGTAAQGIYINSTSGTTGKLLRIRNLG 279


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2736BACYPHPHTASE3031e-99 Salmonella/Yersinia modular tyrosine phosphatase si...
		>BACYPHPHTASE#Salmonella/Yersinia modular tyrosine phosphatase

signature.
Length = 468

Score = 303 bits (777), Expect = 1e-99
Identities = 68/212 (32%), Positives = 102/212 (48%), Gaps = 17/212 (8%)

Query: 340 GKPVALAGSYPKNTPDALEAHMKMLLEKECSCLAVLTSEDQMQAKQ--LPAYFRGSYTFG 397
G +A YP LE+H +ML E LAVL S ++ ++ +P YFR S T+G
Sbjct: 252 GNTRTIACQYP--LQSQLESHFRMLAENRTPVLAVLASSSEIANQRFGMPDYFRQSGTYG 309

Query: 398 EVHTNSQKVSSASQGGAI--DQYNMQL-SCGEKRYTIPVLHVKNWPDHQPLPS--TDQLE 452
+ S+ G I D Y + + G+K ++PV+HV NWPD + S T L
Sbjct: 310 SITVESKMTQQVGLGDGIMADMYTLTIREAGQKTISVPVVHVGNWPDQTAVSSEVTKALA 369

Query: 453 YLADRVKNSNQNGAPGRSSS-----DKHLPMIHCLGGVGRTGTMAAALVLKDNPHSNL-- 505
L D+ + +N + SS K P+IHC GVGRT + A+ + D+ +S L
Sbjct: 370 SLVDQTAETKRNMYESKGSSAVGDDSKLRPVIHCRAGVGRTAQLIGAMCMNDSRNSQLSV 429

Query: 506 EQVRADFRNSRNNRMLEDASQF-VQLKAMQAQ 536
E + + R RN M++ Q V +K + Q
Sbjct: 430 EDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQ 461


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2737PF05932345e-05 Tir chaperone protein (CesT)
		>PF05932#Tir chaperone protein (CesT)

Length = 127

Score = 33.6 bits (77), Expect = 5e-05
Identities = 16/111 (14%), Positives = 40/111 (36%), Gaps = 7/111 (6%)

Query: 4 PLTFDDNNQCLLLLDSDIFTSIEAK--DDIWLLNGMIIPLSPVCGDSIWRQIMVINGELA 61
PL FDD+ C +++D+ ++ + LL G++ P D + ++
Sbjct: 21 PLVFDDHGTCNMIIDNTFALTLSCDYARERLLLIGLLEPH----KDIPQQCLLAGALNPL 76

Query: 62 ANNEGTLAYIDAAETLLFIHAI-TDLTNIYHIISQLESFVNKQEALKNILQ 111
N L + + +I + ++ + ++ + + Q
Sbjct: 77 LNAGPGLGLDEKSGLYHAYQSIPREKLSVPTLKREMAGLLEWMRGWREASQ 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2742BACINVASINC5140.0 Salmonella/Shigella invasin protein C signature.
		>BACINVASINC#Salmonella/Shigella invasin protein C signature.

Length = 409

Score = 514 bits (1324), Expect = 0.0
Identities = 407/409 (99%), Positives = 407/409 (99%)

Query: 1 MLISNVGINPAAYLNNHSVENSSQTASQSVSAKDILNSIGISSSKVSDLGLSPTLSAPAP 60
MLISNVGINPAAYLNNHSVENSSQTASQSVSAKDILNSIGISSSKVSDLGLSPTLSAPAP
Sbjct: 1 MLISNVGINPAAYLNNHSVENSSQTASQSVSAKDILNSIGISSSKVSDLGLSPTLSAPAP 60

Query: 61 GVLTQTPGTITSFLKASIQNTDMNQDLNALANNVTTKANEVVQTQLREQQAEVGKFFDIS 120
GVLTQTPGTITSFLKASIQNTDMNQDLNALANNVTTKANEVVQTQLREQQAEVGKFFDIS
Sbjct: 61 GVLTQTPGTITSFLKASIQNTDMNQDLNALANNVTTKANEVVQTQLREQQAEVGKFFDIS 120

Query: 121 GMSSSAVALLAAANTLMLTLNQADSKLSGKLSLVSFDAAKTTASSMMREGMNALSGSISQ 180
GMSSSAVALLAAANTLMLTLNQADSKLSGKLSLVSFDAAKTTASSMMREGMNALSGSISQ
Sbjct: 121 GMSSSAVALLAAANTLMLTLNQADSKLSGKLSLVSFDAAKTTASSMMREGMNALSGSISQ 180

Query: 181 SALQLGITGVGAKLEYKGLQNERGALKHNAAKIDKLTTESHSIKNVLNGQNSVKLGAEGV 240
SALQLGITGVGAKLEYKGLQNERGALKHNAAKIDKLTTESHSIKNVLNGQNSVKLGAEGV
Sbjct: 181 SALQLGITGVGAKLEYKGLQNERGALKHNAAKIDKLTTESHSIKNVLNGQNSVKLGAEGV 240

Query: 241 DSLKSLNMKKTGTDATKNLNDATLKSNAGTSATESLGIKNSNKQISPEHQAILSKRLESV 300
DSLKSLNMKKTGTDATKNLNDATLKSNAGTSATESLGIKNSNKQISPEHQAILSKRLESV
Sbjct: 241 DSLKSLNMKKTGTDATKNLNDATLKSNAGTSATESLGIKNSNKQISPEHQAILSKRLESV 300

Query: 301 ESDIRLEQNTMDMTRIDARKMQMTGDLIMKNLVTVGGIAGASGQYAATQERSEQQISQVN 360
ESDIRLEQNTMDMTRIDARKMQMTGDLIMKN VTVGGIAGAS QYAATQERSEQQISQVN
Sbjct: 301 ESDIRLEQNTMDMTRIDARKMQMTGDLIMKNSVTVGGIAGASRQYAATQERSEQQISQVN 360

Query: 361 NRVASTASDEARESSRKSTSLIQEMLKTMESINQSKASALAAIAGNIRA 409
NRVASTASDEARESSRKSTSLIQEMLKTMESINQSKASALAAIAGNIRA
Sbjct: 361 NRVASTASDEARESSRKSTSLIQEMLKTMESINQSKASALAAIAGNIRA 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2743BACINVASINB8350.0 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 835 bits (2158), Expect = 0.0
Identities = 590/593 (99%), Positives = 590/593 (99%)

Query: 1 MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGE 60
MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGE
Sbjct: 1 MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGE 60

Query: 61 SAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKE 120
SAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKE
Sbjct: 61 SAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKE 120

Query: 121 MGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAAAKKLTQAQNKLQSLDPADPG 180
MGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAA KKLTQAQNKLQSLDPADPG
Sbjct: 121 MGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAATKKLTQAQNKLQSLDPADPG 180

Query: 181 YAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQN 240
YAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQN
Sbjct: 181 YAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQN 240

Query: 241 QVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEF 300
QVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEF
Sbjct: 241 QVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEF 300

Query: 301 QEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAA 360
QEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAA
Sbjct: 301 QEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAA 360

Query: 361 TGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMV 420
TGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMV
Sbjct: 361 TGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMV 420

Query: 421 AVIAVVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLG 480
AVI VVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLG
Sbjct: 421 AVIVVVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLG 480

Query: 481 NVGSKMGLQTNALSKELVGNTLNKVALSMEVTNTAAQSAGGVAEGVFIKNASEALADFML 540
NVGSKMGLQTNALSKELVGNTLNKVAL MEVTNTAAQSAGGVAEGVFIKNASEALADFML
Sbjct: 481 NVGSKMGLQTNALSKELVGNTLNKVALGMEVTNTAAQSAGGVAEGVFIKNASEALADFML 540

Query: 541 ARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA 593
ARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA
Sbjct: 541 ARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA 593


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2744SYCDCHAPRONE1282e-40 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 128 bits (322), Expect = 2e-40
Identities = 39/160 (24%), Positives = 72/160 (45%), Gaps = 4/160 (2%)

Query: 4 QNNVSEERVAEMIWDAVSEGATLKDVHGIPQDMMDGLYAHAYEFYNQGRLDEAETFFRFL 63
Q + + + G T+ ++ I D ++ LY+ A+ Y G+ ++A F+ L
Sbjct: 3 QETTDTQEYQLAMESFLKGGGTIAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQAL 62

Query: 64 CIYDFYNPDYTMGLAAVCQLKKQFQKACDLYAVAFTLLKNDYRPVFFTGQCQLLMRKAAK 123
C+ D Y+ + +GL A Q Q+ A Y+ + + R F +C L + A+
Sbjct: 63 CVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAE 122

Query: 124 ARQCF----ELVNERTEDESLRAKALVYLEALKTAETEQH 159
A EL+ ++TE + L + LEA+K + +H
Sbjct: 123 AESGLFLAQELIADKTEFKELSTRVSSMLEAIKLKKEMEH 162


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2745TYPE3IMSPROT340e-118 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 340 bits (874), Expect = e-118
Identities = 119/360 (33%), Positives = 204/360 (56%), Gaps = 19/360 (5%)

Query: 1 MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFN-EFMGIIKIII 59
MS KTE+PT K++ D+ KKGQ KSK+++ L + A L+ + E + +I
Sbjct: 1 MSGEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIP 60

Query: 60 ADNFDQSMADYSLAVFGIGLKYLIPFMLLCL---VCSALPAL----LQAGFVLATEALKP 112
+QS +S A+ + L+ F LC +AL A+ +Q GF+++ EA+KP
Sbjct: 61 ---AEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKP 117

Query: 113 NLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIFSQLNGNVVDIA 172
++ +NP+EGAK++FS++++ + +K++L + V+ +I+ W K + + L I
Sbjct: 118 DIKKINPIEGAKRIFSIKSLVEFLKSILKV---VLLSILIWIIIKGNLVTLLQLPTCGIE 174

Query: 173 VIWRELLLALVLTCLACA---LIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEV 229
I L L + C +++ + D EY+ +K++KM K+E+KRE KE EG+PE+
Sbjct: 175 CITPLLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEI 234

Query: 230 KSKRREVHMEILSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALA 289
KSKRR+ H EI S ++ +++ S ++VANPTHI IGI +K P+P+++ T+ +
Sbjct: 235 KSKRRQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQT 294

Query: 290 VRAYAEKVGVPVIVDIKLARSLFKTHRRYDLVSLEEIDEVLRLLVWLE--EVENAGKDVI 347
VR AE+ GVP++ I LAR+L+ + E+I+ +L WLE +E +++
Sbjct: 295 VRKIAEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNIEKQHSEML 354


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2746TYPE3IMRPROT1882e-61 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 188 bits (479), Expect = 2e-61
Identities = 50/248 (20%), Positives = 107/248 (43%), Gaps = 4/248 (1%)

Query: 1 MLYALYFEIHHLVASAALGFARVAPIFFFLPFLNSGVLSGAPRNAIIILVALGVWPHALN 60
ML + + RV + P L+ + + + +++ + P
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 EAPPFLSVAMIPLVLQEAAVGVMLGCLLSWPFWVMHALGCIIDNQRGATLSSSIDPANGI 120
P S + L +Q+ +G+ LG + + F + G II Q G + ++ +DPA+ +
Sbjct: 61 NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHL 120

Query: 121 DTSEMANFLNMFAAVVYLQNGGLVTMVDVLNKSYQLCDPMNEC--TPSLPPLLTFINQVA 178
+ +A ++M A +++L G + ++ +L ++ E + + L + +
Sbjct: 121 NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIF 180

Query: 179 QNALVLASPVVLVLLLSEVFLGLLSRFAPQMNAFAISLTVKSGIAVLIMLLYFS--PVLP 236
N L+LA P++ +LL + LGLL+R APQ++ F I + + + +M
Sbjct: 181 LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC 240

Query: 237 DNVLRLSF 244
+++ F
Sbjct: 241 EHLFSEIF 248


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2747TYPE3IMQPROT894e-27 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 88.7 bits (220), Expect = 4e-27
Identities = 86/86 (100%), Positives = 86/86 (100%)

Query: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL 60
MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL
Sbjct: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL 60

Query: 61 FLLSGWYGEVLLSYGRQVIFLALAKG 86
FLLSGWYGEVLLSYGRQVIFLALAKG
Sbjct: 61 FLLSGWYGEVLLSYGRQVIFLALAKG 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2748TYPE3IMPPROT303e-107 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 303 bits (777), Expect = e-107
Identities = 223/224 (99%), Positives = 223/224 (99%)

Query: 1 MGNDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLS 60
MGNDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLS
Sbjct: 1 MGNDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLS 60

Query: 61 MFVMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLSKYSDRELVQFFENAQL 120
MFVMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYL KYSDRELVQFFENAQL
Sbjct: 61 MFVMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLIKYSDRELVQFFENAQL 120

Query: 121 KRQYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVL 180
KRQYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVL
Sbjct: 121 KRQYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVL 180

Query: 181 LALGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQYMDIAT 224
LALGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQYMDIAT
Sbjct: 181 LALGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQYMDIAT 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2749TYPE3OMOPROT5370.0 Type III secretion system outer membrane O protein ...
		>TYPE3OMOPROT#Type III secretion system outer membrane O protein

family signature.
Length = 303

Score = 537 bits (1384), Expect = 0.0
Identities = 302/303 (99%), Positives = 302/303 (99%)

Query: 1 MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL 60
MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL
Sbjct: 1 MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL 60

Query: 61 EHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL 120
EHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL
Sbjct: 61 EHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL 120

Query: 121 HIMSDRGGLWFEHLPELPAVAGGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS 180
HIMSDRGGLWFEHLPELPAV GGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS
Sbjct: 121 HIMSDRGGLWFEHLPELPAVGGGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS 180

Query: 181 RAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR 240
RAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR
Sbjct: 181 RAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR 240

Query: 241 KNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG 300
KNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG
Sbjct: 241 KNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG 300

Query: 301 NGE 303
NGE
Sbjct: 301 NGE 303


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2750SSPANPROTEIN6010.0 Salmonella invasion protein InvJ signature.
		>SSPANPROTEIN#Salmonella invasion protein InvJ signature.

Length = 336

Score = 601 bits (1550), Expect = 0.0
Identities = 332/336 (98%), Positives = 334/336 (99%)

Query: 1 MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSGDKKDRDYGDAFVMHKETAL 60
MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSGDKKDRDYGDAFVMHKETAL
Sbjct: 1 MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSGDKKDRDYGDAFVMHKETAL 60

Query: 61 PVLLAAWRHGAPAKSEHHNGNVSGLHHNGKGELRIAEKLLKVTAEKSVGLISAEAKVDKS 120
P+LLAAWRHGAPAKSEHHNGNVSGLHHNGK ELRIAEKLLKVTAEKSVGLISAEAKVDKS
Sbjct: 61 PLLLAAWRHGAPAKSEHHNGNVSGLHHNGKSELRIAEKLLKVTAEKSVGLISAEAKVDKS 120

Query: 121 AALLSPKNRPLESVSGKKLSADLKAVESVSEVTDNATGISDDNIKALPGDNKAIAGEGVR 180
AALLS KNRPLESVSGKKLSADLKAVESVSEVTDNATGISDDNIKALPGDNKAIAGEGVR
Sbjct: 121 AALLSSKNRPLESVSGKKLSADLKAVESVSEVTDNATGISDDNIKALPGDNKAIAGEGVR 180

Query: 181 KEGAPLARDVAPARMAAANTGKPDDKDHKKVKDVSQLPLQPTTIADLSQLTGGDEKMPLA 240
KEGAPLARDVAPARMAAANTGKP+DKDHKKVKDVSQLPLQPTTIADLSQLTGGDEKMPLA
Sbjct: 181 KEGAPLARDVAPARMAAANTGKPEDKDHKKVKDVSQLPLQPTTIADLSQLTGGDEKMPLA 240

Query: 241 AQSKPMMTIFPTADGVKGEDSSLTYRFQRWGNDYSVNIQARQAGEFSLIPSNTQVEHRLH 300
AQSKPMMTIFPTADGVKGEDSSLTYRFQRWGNDYSVNIQARQAGEFSLIPSNTQVEHRLH
Sbjct: 241 AQSKPMMTIFPTADGVKGEDSSLTYRFQRWGNDYSVNIQARQAGEFSLIPSNTQVEHRLH 300

Query: 301 DQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA 336
DQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA
Sbjct: 301 DQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2751SSPAMPROTEIN1672e-56 Salmonella surface presentation of antigen gene typ...
		>SSPAMPROTEIN#Salmonella surface presentation of antigen gene type M

signature.
Length = 147

Score = 167 bits (423), Expect = 2e-56
Identities = 141/147 (95%), Positives = 143/147 (97%)

Query: 1 MHSLTRIKVLQRRCTVFHSQCESILLRYQDEDRGLQAEEEAILEQIAGLKLLLDTLRAEN 60
MHSLTRIKVLQRRCTVFHSQCESILLRYQDEDR LQ EEEAI+EQIAGLKLLLDTLRAEN
Sbjct: 1 MHSLTRIKVLQRRCTVFHSQCESILLRYQDEDRRLQVEEEAIVEQIAGLKLLLDTLRAEN 60

Query: 61 RQLSREEIYTLLRKQSIVRRQIKDLELQIIQIQEKRSELEKKREEFQKKSKYWLRKEGNY 120
RQLSREEIY LLRKQSIVRRQIKDLELQIIQIQEKRSELEKKREEFQ+KSKYWLRKEGNY
Sbjct: 61 RQLSREEIYALLRKQSIVRRQIKDLELQIIQIQEKRSELEKKREEFQEKSKYWLRKEGNY 120

Query: 121 QRWIIRQKRHYIQREIQQEEAESEEII 147
QRWIIRQKR YIQREIQQEEAESEEII
Sbjct: 121 QRWIIRQKRLYIQREIQQEEAESEEII 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2753SSPAKPROTEIN2057e-72 Invasion protein B family signature.
		>SSPAKPROTEIN#Invasion protein B family signature.

Length = 133

Score = 205 bits (523), Expect = 7e-72
Identities = 43/133 (32%), Positives = 76/133 (57%)

Query: 1 MQHLDIAELVRSALEVSGCDPSLIGGIDSHSTIVLDLFALPSICISVKDDDVWIWAQLGA 60
M ++++ +LVR +L GC PS+I +DSHS I + L ++P+I I++ ++ V +WA A
Sbjct: 1 MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAINIALVNEQVMLWANFDA 60

Query: 61 GSMVVLQQRAYEILMTIMEGCHFARGGQLLLGEQNGELTLKALVHPDFLSDGEKFSTALN 120
S V LQ AY IL ++ ++ + L + L L+ ++ D++ DG F+ L+
Sbjct: 61 PSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRVVIKDDYVHDGIVFAEILH 120

Query: 121 GFYNYLEVFSRSL 133
FY +E+ + L
Sbjct: 121 EFYQRMEILNGVL 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2755INVEPROTEIN6040.0 Salmonella/Shigella invasion protein E (InvE) signat...
		>INVEPROTEIN#Salmonella/Shigella invasion protein E (InvE)

signature.
Length = 372

Score = 604 bits (1557), Expect = 0.0
Identities = 372/372 (100%), Positives = 372/372 (100%)

Query: 1 MIPGSTSGISFSRILSRQASHQDATQHTDAQQAEIQQAAEDSSPGAEVQKFVQSTDEMSA 60
MIPGSTSGISFSRILSRQASHQDATQHTDAQQAEIQQAAEDSSPGAEVQKFVQSTDEMSA
Sbjct: 1 MIPGSTSGISFSRILSRQASHQDATQHTDAQQAEIQQAAEDSSPGAEVQKFVQSTDEMSA 60

Query: 61 ALAQFRNRRDYEKKSSNLSNSFERVLEDEALPKAKQILKLISVHGGALEDFLRQARSLFP 120
ALAQFRNRRDYEKKSSNLSNSFERVLEDEALPKAKQILKLISVHGGALEDFLRQARSLFP
Sbjct: 61 ALAQFRNRRDYEKKSSNLSNSFERVLEDEALPKAKQILKLISVHGGALEDFLRQARSLFP 120

Query: 121 DPSDLVLVLRELLRRKDLEEIVRKKLESLLKHVEEQTDPKTLKAGINCALKARLFGKTLS 180
DPSDLVLVLRELLRRKDLEEIVRKKLESLLKHVEEQTDPKTLKAGINCALKARLFGKTLS
Sbjct: 121 DPSDLVLVLRELLRRKDLEEIVRKKLESLLKHVEEQTDPKTLKAGINCALKARLFGKTLS 180

Query: 181 LKPGLLRASYRQFIQSESHEVEIYSDWIASYGYQRRLVVLDFIEGSLLTDIDANDASCSR 240
LKPGLLRASYRQFIQSESHEVEIYSDWIASYGYQRRLVVLDFIEGSLLTDIDANDASCSR
Sbjct: 181 LKPGLLRASYRQFIQSESHEVEIYSDWIASYGYQRRLVVLDFIEGSLLTDIDANDASCSR 240

Query: 241 LEFGQLLRRLTQLKMLRSADLLFVSTLLSYSFTKAFNAEESSWLLLMLSLLQQPHEVDSL 300
LEFGQLLRRLTQLKMLRSADLLFVSTLLSYSFTKAFNAEESSWLLLMLSLLQQPHEVDSL
Sbjct: 241 LEFGQLLRRLTQLKMLRSADLLFVSTLLSYSFTKAFNAEESSWLLLMLSLLQQPHEVDSL 300

Query: 301 LADIIGLNALLLSHKEHASFLQIFYQVCKAIPSSLFYEEYWQEELLMALRSMTDIAYKHE 360
LADIIGLNALLLSHKEHASFLQIFYQVCKAIPSSLFYEEYWQEELLMALRSMTDIAYKHE
Sbjct: 301 LADIIGLNALLLSHKEHASFLQIFYQVCKAIPSSLFYEEYWQEELLMALRSMTDIAYKHE 360

Query: 361 MAEQRRTIEKLS 372
MAEQRRTIEKLS
Sbjct: 361 MAEQRRTIEKLS 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2756TYPE3OMGPROT5760.0 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 576 bits (1486), Expect = 0.0
Identities = 169/540 (31%), Positives = 271/540 (50%), Gaps = 57/540 (10%)

Query: 4 HILLARVLACAALVLVAPGYSSE----KIPVTGSGFVAKDDSLRTFFDAMALQLKEPVIV 59
H RVL L+L + ++ E IP +VAK +SLR V+V
Sbjct: 6 HSFFKRVLTGTLLLLSSYSWAQELDWLPIPYV---YVAKGESLRDLLTDFGANYDATVVV 62

Query: 60 SKMAARKKITGNFEFHDPNALLEKLSLQLGLIWYFDGQAIYIYDASEMRNAVVSLRNVSL 119
S K++G FE +P L+ ++ L+WY+DG +YI+ SE+ + ++ L+
Sbjct: 63 SD-KINDKVSGQFEHDNPQDFLQHIASLYNLVWYYDGNVLYIFKNSEVASRLIRLQESEA 121

Query: 120 NEFNNFLKRSGLYNKNYPLRGDNRKGTFYVSGPPVYVDMVVNAATMMDKQND--GIELGR 177
E L+RSG++ + R D YVSGPP Y+++V A +++Q + G
Sbjct: 122 AELKQALQRSGIWEPRFGWRPDASNRLVYVSGPPRYLELVEQTAAALEQQTQIRSEKTGA 181

Query: 178 QKIGVMRLNNTFVGDRTYNLRDQKMVIPGIATAIERLLQGEEQPLGNIVSSEPPAMPAFS 237
I + L DRT + RD ++ PG+AT ++R+L + + P
Sbjct: 182 LAIEIFPLKYASASDRTIHYRDDEVAAPGVATILQRVLSDATIQQVTVDNQRIP------ 235

Query: 238 ANGEKGKAANYAGGMSLQEALKQNAAAGNIKIVAYPDTNSLLVKGTAEQVHFIEMLVKAL 297
Q A + +A A ++ A P N+++V+ + E++ + L+ AL
Sbjct: 236 -----------------QAATRASAQA---RVEADPSLNAIIVRDSPERMPMYQRLIHAL 275

Query: 298 DVAKRHVELSLWIVDLNKSDLERLGTSWSGSI-----------TIGDKLGVSLNQSSIST 346
D +E++L IVD+N L LG W I T GD+ ++ N + S
Sbjct: 276 DKPSARIEVALSIVDINADQLTELGVDWRVGIRTGNNHQVVIKTTGDQSNIASNGALGSL 335

Query: 347 LDG---SRFIAAVNALEEKKQATVVSRPVLLTQENVPAIFDNNRTFYTKLIGERNVALEH 403
+D +A VN LE + A VVSRP LLTQEN A+ D++ T+Y K+ G+ L+
Sbjct: 336 VDARGLDYLLARVNLLENEGSAQVVSRPTLLTQENAQAVIDHSETYYVKVTGKEVAELKG 395

Query: 404 VTYGTMIRVLPRFSADG---QIEMSLDIEDGNDKTPQSDTTTSVDALPEVGRTLISTIAR 460
+TYGTM+R+ PR G +I ++L IEDGN Q ++ ++ +P + RT++ T+AR
Sbjct: 396 ITYGTMLRMTPRVLTQGDKSEISLNLHIEDGN----QKPNSSGIEGIPTISRTVVDTVAR 451

Query: 461 VPHGKSLLVGGYTRDANTDTVQSIPFLGKLPLIGSLFRYSSKNKSNVVRVFMIEPKEIVD 520
V HG+SL++GG RD + + +P LG +P IG+LFR S+ VR+F+IEP+ I +
Sbjct: 452 VGHGQSLIIGGIYRDELSVALSKVPLLGDIPYIGALFRRKSELTRRTVRLFIIEPRIIDE 511


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2768TCRTETB832e-19 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 82.6 bits (204), Expect = 2e-19
Identities = 65/387 (16%), Positives = 142/387 (36%), Gaps = 48/387 (12%)

Query: 16 FLDLINLFIASVAFPAMSVDLHTSISALAWVSNGYIAGLTLIVPFSAFLSRYLGARRLII 75
F ++N + +V+ P ++ D + ++ WV+ ++ ++ LS LG +RL++
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 76 FSLILFSVAAAAAGFADSLHS-LVFWRIVQGAGGGLLIPVGQALTWQQFEPHERAGVSSV 134
F +I+ + S S L+ R +QGAG + + + R +
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 135 VMMVALLAPACSPAIGGLLVETCGWRWIFFATLPVAVLTLLLAYCWLNAASTT------- 187
+ + + PAIGG++ W ++ + + L
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKG 203

Query: 188 --------------MASARLLHL-------------------PLLTDRLLRFAMIVYLCV 214
S + L P + L + + +
Sbjct: 204 IILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVL 263

Query: 215 PGMFIGISVVGM-----FYLQNIAQLSPAAAGS-LMLPWSIASFVAIMLTGRYFNRLGPR 268
G I +V G + ++++ QLS A GS ++ P +++ + + G +R GP
Sbjct: 264 CGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPL 323

Query: 269 PLIIVGCLLQAAGILLLTNVTPATSHRVLMMIFALMGAGGSLCSSTAQSGAFLTIARRDM 328
++ +G + L + + TS + ++I ++G G S + + ++ +++
Sbjct: 324 YVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSFTKTVISTIVSSSLKQQEA 382

Query: 329 PDASALWNLNRQLSFFLGATLLTLLLN 355
+L N LS G ++ LL+
Sbjct: 383 GAGMSLLNFTSFLSEGTGIAIVGGLLS 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2771NUCEPIMERASE889e-22 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 87.5 bits (217), Expect = 9e-22
Identities = 56/219 (25%), Positives = 93/219 (42%), Gaps = 31/219 (14%)

Query: 1 MQIIITGGGGFLGQKLASALLNSSL------AFNELLLVDLKMPARLS--DSPRLRCLEA 52
M+ ++TG GF+G ++ LL + N+ V LK ARL P + +
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQ-ARLELLAQPGFQFHKI 59

Query: 53 DLT-QPGVLENVITANTSVVYHLAA-------IVSSHAEDDFDLGWKVNLDLTRQLLEAC 104
DL + G+ + + + V+ + + HA D NL +LE C
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYAD------SNLTGFLNILEGC 113

Query: 105 RRQPQKIRFVFSSSLAVYGG--TLPECVTDTTALTPRSSYGAQKAACELLVNDYTRKGYV 162
R + +++SS +VYG +P D+ P S Y A K A EL+ + Y+ +
Sbjct: 114 RHNKIQ-HLLYASSSSVYGLNRKMPFSTDDSVD-HPVSLYAATKKANELMAHTYSHLYGL 171

Query: 163 DGLALRLPTICVRPGKPNRAASSFVSAIIREPLQGETIV 201
LR T+ G+P+ A F A+ L+G++I
Sbjct: 172 PATGLRFFTVYGPWGRPDMALFKFTKAM----LEGKSID 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2781RTXTOXIND320.004 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.1 bits (73), Expect = 0.004
Identities = 17/84 (20%), Positives = 36/84 (42%), Gaps = 12/84 (14%)

Query: 290 IVATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGS 349
IVATA+G++ ++G + IK ++ ++V+E + V+ G + + +
Sbjct: 82 IVATANGKLTHSGRSK-------EIKP---IENSIV--KEIIVKEGESVRKGDVLLKLTA 129

Query: 350 TGTSSTRLHFEIRYKGKSVNPLRY 373
G + L + + RY
Sbjct: 130 LGAEADTLKTQSSLLQARLEQTRY 153


43SPA2794SPA2817Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2794-225-4.132009hypothetical protein
SPA2795018-3.571585hypothetical protein
SPA2796-114-1.289928hypothetical protein
SPA2797013-0.026220hypothetical protein
SPA27980150.609647hypothetical protein
SPA28010140.701987hypothetical protein
SPA28022262.2741183'-phosphoadenosine 5'-phosphosulfate
SPA28031261.413752sulfite reductase (NADPH) hemoprotein subunit
SPA28042250.479006sulfite reductase (NADPH) flavoprotein subunit
SPA2805125-1.4318576-pyruvoyl tetrahydrobiopterin synthase
SPA2807021-0.091928hypothetical protein
SPA28090210.174843enolase
SPA2810-1130.552329CTP synthetase
SPA28110110.763117hypothetical protein
SPA28122120.398635fimbrial subunit
SPA2813113-1.477546outer membrane usher protein
SPA2814425-5.831165periplasmic fimbrial chaperone
SPA2815532-7.192197fimbrial subunit
SPA2816019-4.320321fimbrial subunit
SPA2817-217-3.362467fimbrial subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2795PHPHTRNFRASE290.025 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 28.6 bits (64), Expect = 0.025
Identities = 9/36 (25%), Positives = 14/36 (38%), Gaps = 5/36 (13%)

Query: 90 QLRANPVITRNGKRSDVMMNAKH-----QAKANGVE 120
+L P T++G ++ N ANG E
Sbjct: 256 KLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGE 291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2809ANTHRAXTOXNA290.036 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 29.3 bits (65), Expect = 0.036
Identities = 31/132 (23%), Positives = 51/132 (38%), Gaps = 9/132 (6%)

Query: 211 GYAPNLGSNAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLA-----GEGN 265
P L N + A+ +E K YE+GK I+L + + ++ + +
Sbjct: 147 RETPKLIINIKDYAINSEQSKEVYYEIGKGISLDIISKDKSLDPEFLNLIKSLSDDSDSS 206

Query: 266 KAFTSEEFTHFLEELTKQYPIVSIEDGLDESDW---DGFAYQTKVLG-DKIQLVGDDLFV 321
S++F LE K I I++ L E F+Y ++L D+F
Sbjct: 207 DLLFSQKFKEKLELNNKSIDINFIKENLTEFQHAFSLAFSYYFAPDHRTVLELYAPDMFE 266

Query: 322 TNTKILKEGIEK 333
K+ K G EK
Sbjct: 267 YMNKLEKGGFEK 278


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2813PF005777020.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 702 bits (1814), Expect = 0.0
Identities = 216/864 (25%), Positives = 369/864 (42%), Gaps = 68/864 (7%)

Query: 5 ASPYSASGKDIEFNTDFLDVKNRDNVNIAQFSRKGFILPGVYLLQIKINGQTLPQEFPVN 64
A+ S ++ FN FL + ++++F + PG Y + I +N + V
Sbjct: 37 AAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATR-DVT 95

Query: 65 WVIPEHDPQGSEVCAEPELVTQLGIKPELAEKLVWITHGERQCLAPDSL-KGMDFQADLG 123
+ QG C + +G+ + + + C+ S+ Q D+G
Sbjct: 96 FN-TGDSEQGIVPCLTRAQLASMGLNTASVSGMNLL--ADDACVPLTSMIHDATAQLDVG 152

Query: 124 HSTLLVNLPQAYMEYSDVDWDPPARWDNGIPGIILDYNINNQLRHDQESGSEEQSISGNG 183
L + +PQA+M + PP WD GI +L+YN + ++ G+ N
Sbjct: 153 QQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNS-HYAYLNL 211

Query: 184 TLGANLGAWRLRADWQASYDHRDDDENTSTLHDQSWSRYYAYRALPTLGAKLTLGESYLQ 243
G N+GAWRLR + SY+ D + + R + L ++LTLG+ Y Q
Sbjct: 212 QSGLNIGAWRLRDNTTWSYNSSDSSSGSKN--KWQHINTWLERDIIPLRSRLTLGDGYTQ 269

Query: 244 SDVFDSFNYIGASVVSDDQMLPPKLRGYAPEIVGIARSNAKVKVSWQGRVLYETQVPAGP 303
D+FD N+ GA + SDD MLP RG+AP I GIAR A+V + G +Y + VP GP
Sbjct: 270 GDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGP 329

Query: 304 FRIQDLNQ-SVSGTLHVTVEEQNGQTQEFDVNTASVPFLTRPGMVRYKMALGRPQDWDHH 362
F I D+ SG L VT++E +G TQ F V +SVP L R G RY + G + +
Sbjct: 330 FTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQ 389

Query: 363 PITGTFASAEASWGVTNGWSLYGGAIGESNYQAVALGSGKDLGVVGAVAVDITHSIAHMP 422
F + G+ GW++YGG Y+A G GK++G +GA++VD+T + + +P
Sbjct: 390 QEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLP 449

Query: 423 QDDGFDGETLQGNSYRISYSRDFDEIDSRLTFAGYRFSEKNFMSMSDYLDAKT--YHHLN 480
D G S R Y++ +E + + GYR+S + + +D ++ Y+
Sbjct: 450 DD-----SQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIET 504

Query: 481 A-----------------GHEKERYTVTYNQNFREQGMSAYFSYSRSTFWDSPDQS-NYN 522
+++ + +T Q + Y S S T+W + + +
Sbjct: 505 QDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTS-TLYLSGSHQTYWGTSNVDEQFQ 563

Query: 523 LSLSWYFDLGSIKNLSASLNGYRSEYNGDKDDGVYISLSVPWG------------NDSIS 570
L+ F+ + + S + ++ + +D + +++++P+ + S S
Sbjct: 564 AGLNTAFEDIN---WTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASAS 620

Query: 571 YNGT-FNGSQHRNQLGYSGH--SQNGDNWQLHVG-----QDEQGAQADGYYSHQGALTDI 622
Y+ + + N G G N ++ + G G+ +++G +
Sbjct: 621 YSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNA 680

Query: 623 DLSADYEEGSYRSLGMSLRGGMTLTTQGGALHRGSLAGSTRLLVDTDGIADVPVSGNGSP 682
++ + + + L + GG+ G L + T +LV G D V N +
Sbjct: 681 NIGYSHSDD-IKQLYYGVSGGVLAHANGVTLGQPL--NDTVVLVKAPGAKDAKV-ENQTG 736

Query: 683 TSTNIFGKAVIADVGSYSRSLARIDLNKLPEKAEATKSVVQITLTEGAIGYRHFDVVSGE 742
T+ G AV+ Y + +D N L + + +V + T GAI F G
Sbjct: 737 VRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGI 796

Query: 743 KMMAVFRLADGDFPPFGAEVKNERQQQLGLVADDGNAWLAGVKAGETLKVFW--DGAAQC 800
K++ + PFGA V +E Q G+VAD+G +L+G+ ++V W + A C
Sbjct: 797 KLLMTLT-HNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHC 855

Query: 801 EA--SLPPTFTPELLANALLLPCK 822
A LPP +LL L C+
Sbjct: 856 VANYQLPPESQQQLL-TQLSAECR 878


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2815FIMBRIALPAPF342e-04 Escherichia coli: P pili tip fibrillum papF protein...
		>FIMBRIALPAPF#Escherichia coli: P pili tip fibrillum papF protein

signature.
Length = 167

Score = 33.5 bits (76), Expect = 2e-04
Identities = 36/144 (25%), Positives = 61/144 (42%), Gaps = 26/144 (18%)

Query: 39 PPCTVGGAS---VEFGDVLTTKVGDVSQTKPVGYSLNCDGRASDYLKLQIQGTTTTISGE 95
PPCT+ V+FG++ V + S++C + S L +++ G T +
Sbjct: 32 PPCTINNGQNIVVDFGNINPEHVDNSRGEVTKNISISCPYK-SGSLWIKVTGNTMGVGQN 90

Query: 96 QVLQTSVQGLGIRIQQ-------------AGNKQLVPVGI-TDWLNFTLSGSNGPELEAV 141
VL T++ GI + Q +GN V G+ T FT + +V
Sbjct: 91 NVLATNITHFGIALYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFT--------SV 142

Query: 142 PVKEPTTQLAGGDFNASATLVVDY 165
P + + L GGDF +A++ + Y
Sbjct: 143 PFRNGSGILNGGDFRTTASMSMIY 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2816FIMBRIALPAPF376e-06 Escherichia coli: P pili tip fibrillum papF protein...
		>FIMBRIALPAPF#Escherichia coli: P pili tip fibrillum papF protein

signature.
Length = 167

Score = 37.4 bits (86), Expect = 6e-06
Identities = 43/166 (25%), Positives = 71/166 (42%), Gaps = 20/166 (12%)

Query: 5 LILTLLITRFAC-AD-NLTFHGKLINPPACTINNGETLEVSFGSVIIDNIDGVNYLTEIP 62
L ++LL+T A AD + G + PP CTINNG+ + V FG++ +++D N E+
Sbjct: 6 LFISLLLTSVAVLADVQINIRGNVYIPP-CTINNGQNIVVDFGNINPEHVD--NSRGEVT 62

Query: 63 WTLTCDSSFRDDALTFTLSYLGTATPYSAKALTTNVPGLGIELQQNGTVFPPGT------ 116
++ ++ +L ++ T L TN+ GI L Q + P T
Sbjct: 63 KNISISCPYKSGSLWIKVTG-NTMGVGQNNVLATNITHFGIALYQGKGMSTPLTLGNGSG 121

Query: 117 -------SLTINESSLPTLKAVPVKQPGKEPAEGDFEAFATLQVDY 155
L S+ T +VP + GDF A++ + Y
Sbjct: 122 NGYRVTAGLDTARSTF-TFTSVPFRNGSGILNGGDFRTTASMSMIY 166


44SPA2889SPA2900Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2889323-4.754926amino acid transport protein
SPA2890526-2.525547hypothetical protein
SPA28917261.599246inner membrane protein
SPA28927281.662699hypothetical protein
SPA28937250.654200hypothetical protein
SPA28947250.118865hypothetical protein
SPA28956282.407857fimbrial chaperone protein
SPA28966261.499957outer membrane fimbrial usher protein
SPA2897727-5.165876fimbrial protein
SPA2898627-5.206206hypothetical protein
SPA2900223-2.843795transposase for insertion sequence element
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2896PF005776320.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 632 bits (1631), Expect = 0.0
Identities = 228/856 (26%), Positives = 380/856 (44%), Gaps = 66/856 (7%)

Query: 19 SQATEFNASLLDSGNLSNVDLTAFSREGYVAPGNYILDIWLNDQPVREQYPVRVVPVAGR 78
S FN L + DL+ F + PG Y +DI+LN+ + + V
Sbjct: 44 SAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRD-VTFNTGDSE 102

Query: 79 DAAVICVTTDMVAMLGLKDKIIHGLKPVTGIPDGQCLELRSA--DSQVRYSAENQRLTFI 136
V C+T +A +GL + + + D C+ L S D+ + QRL
Sbjct: 103 QGIVPCLTRAQLASMGLNTA---SVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLT 159

Query: 137 IPQAWMRYQDPDWVPPSRWSDGVTAGLLDYSLMANRYMPQQGETSTSYSLYGTAGFNLGA 196
IPQA+M + ++PP W G+ AGLL+Y+ N + G S L +G N+GA
Sbjct: 160 IPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGA 219

Query: 197 WRLRSDYQYSRFDS-GQGASQSDFYLPQTYLFRALPALRSKLTLGQTYLSSAIFDSFRFA 255
WRLR + +S S S++ + T+L R + LRS+LTLG Y IFD F
Sbjct: 220 WRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGINFR 279

Query: 256 GLTLTSDERMLPPSLQGYAPKISGIANSNAQVTVSQNGRILYQTRVSPGPFELPDLSQ-N 314
G L SD+ MLP S +G+AP I GIA AQVT+ QNG +Y + V PGPF + D+
Sbjct: 280 GAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAG 339

Query: 315 ISGNLDVSVRESDGSVRTWQVNTASVPFIARQGQVRYKVAAGRPLYGGTHNNSTVSPDFL 374
SG+L V+++E+DGS + + V +SVP + R+G RY + AG G N P F
Sbjct: 340 NSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSG---NAQQEKPRFF 396

Query: 375 LGEATWGAFNNTSLYGGLIASTGDYQSAALGIGQNMGLLGALSADVTRSDARLPHGKKQS 434
G ++YGG + Y++ GIG+NMG LGALS D+T++++ LP +
Sbjct: 397 QSTLLHGLPAGWTIYGGTQLA-DRYRAFNFGIGKNMGALGALSVDMTQANSTLPDDSQHD 455

Query: 435 GYSYRINYAKTFDKTGSTLAFVGYRFSDRHFLSMPEYLQRRATDGGD------------- 481
G S R Y K+ +++G+ + VGYR+S + + + R
Sbjct: 456 GQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKF 515

Query: 482 ------AWHEKQSYTVTYSQSVPVLNMSAALSVSRLNYWNAQ-SNNNYMLSFNKVFSLGD 534
A++++ +T +Q + + LS S YW + + N F
Sbjct: 516 TDYYNLAYNKRGKLQLTVTQQLG-RTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAF---- 570

Query: 535 LQGLSASVSFARNQYTGG-GSQNQVYATISIPWGDSR-----------QVSYSVQKDNRG 582
+ ++ ++S++ + G + ++IP+ SYS+ D G
Sbjct: 571 -EDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNG 629

Query: 583 GLQQTVNYSD--FHNPDTTWNISAGHNRYDTGSN-SSFSGSVQSRLPWGQAAADATLQPG 639
+ + + ++++ G+ G++ S+ ++ R +G A +
Sbjct: 630 RMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDD 689

Query: 640 QYRSLGLSWYGSVTATAHGAAFSQSMAGNEPRMMIDTGDVAGVPVNGNSGV-TNRFGVGV 698
+ L G V A A+G Q + N+ +++ V +GV T+ G V
Sbjct: 690 -IKQLYYGVSGGVLAHANGVTLGQPL--NDTVVLVKAPGAKDAKVENQTGVRTDWRGYAV 746

Query: 699 VSAGSSYRRSDISVDVAALPEDVDVSSSVVSQVLTEGAVGYRKIDASQGAQVLGHIRLAD 758
+ + YR + +++D L ++VD+ ++V + V T GA+ + A G ++L + +
Sbjct: 747 LPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLT-HN 805

Query: 759 GASPPFGALVVSGKTGRTAGMVGDDGLAYLTGLSGEDRRTLNVSW--DGRVQCRLTLPET 816
PFGA+V S +++G+V D+G YL+G+ + V W + C
Sbjct: 806 NKPLPFGAMVTSES-SQSSGIVADNGQVYLSGM--PLAGKVQVKWGEEENAHCVANYQLP 862

Query: 817 VTLSRGPL---LLPCR 829
+ L CR
Sbjct: 863 PESQQQLLTQLSAECR 878


45SPA2976SPA2997Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2976010-3.192182nucleoside permease
SPA2977116-4.683934ornithine decarboxylase isozyme
SPA2978338-13.308704hypothetical protein
SPA29801657-20.874544*hypothetical protein
SPA29811860-21.597078hypothetical protein
SPA29821857-21.143475hypothetical protein
SPA29831656-20.864556bacteriocin immunity protein
SPA29841455-20.812423bacteriocin immunity protein
SPA29851255-21.017454hypothetical protein
SPA29861148-19.362467hypothetical protein
SPA2987742-16.761160hypothetical protein
SPA2988438-13.845677hypothetical protein
SPA2989326-6.665634hypothetical protein
SPA2990426-5.927697membrane protein
SPA2991223-4.827181hypothetical protein
SPA2992124-4.013170LysR family transcriptional regulator
SPA2993222-3.261790hypothetical protein
SPA2994120-2.809370amino acid transport protein
SPA2995122-3.692500hypothetical protein
SPA2996123-4.126474oxidoreductase
SPA2997117-3.230491aldehyde dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2995PRTACTNFAMLY260.030 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 25.8 bits (56), Expect = 0.030
Identities = 8/18 (44%), Positives = 11/18 (61%)

Query: 50 RFSPGDSWFVEQGTEVAW 67
RF+ D WF+E E+A
Sbjct: 773 RFTHADGWFLEPQAELAV 790


46SPA3135SPA3163Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA31352151.598622phosphoheptose isomerase
SPA31361182.593489lipoprotein
SPA31370181.729183hypothetical protein
SPA31380162.445156hypothetical protein
SPA31391172.965956hypothetical protein
SPA31401172.536347hypothetical protein
SPA31410203.236308acetyltransferase
SPA31421172.120399hypothetical protein
SPA31431252.570792protease
SPA31443291.928430hypothetical protein
SPA31463301.853922amino acid permease
SPA31475341.784592ATP-dependent RNA helicase
SPA31485331.140745hypothetical protein
SPA31495361.470551polynucleotide phosphorylase
SPA31515300.80018230S ribosomal protein S15
SPA31525280.794012tRNA pseudouridine 55 synthase (psi55 synthase)
SPA31535290.385681ribosome-binding factor A (P15B protein)
SPA31543260.983096protein chain initiation factor 2
SPA31551170.394364L factor
SPA31562191.344912hypothetical protein
SPA31581181.227798*argininosuccinate synthetase
SPA31600161.097367*protein-export membrane protein
SPA31611170.963851PGM/PMM-family protein
SPA31620150.412953dihydropteroate synthase
SPA31632170.726846cell division protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3135RTXTOXINA280.034 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 27.6 bits (61), Expect = 0.034
Identities = 26/111 (23%), Positives = 44/111 (39%), Gaps = 22/111 (19%)

Query: 42 NKILCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIA-------NDRL 94
K+L GN + A T + IA + V AI+ D+
Sbjct: 277 TKVL--GNVGKGISQYIIAQRAAQGLSTSAAAAGLIA----SAVTLAISPLSFLSIADKF 330

Query: 95 HD----EVYAKQVRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVA 141
E Y+++ + LG+ GD LLA + A++A++T T++A
Sbjct: 331 KRANKIEEYSQRFKKLGYDGDSLLAAFHKETG-----AIDASLTTISTVLA 376


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3137NUCEPIMERASE290.009 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 29.4 bits (66), Expect = 0.009
Identities = 14/55 (25%), Positives = 22/55 (40%), Gaps = 16/55 (29%)

Query: 4 VLITGATGLVGGHLLRMLINTPQVSAIAAPTRRPLTDIVGV--YNP-HDPQLTDA 55
L+TGA G +G H+ + L+ +VG+ N +D L A
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGH-------------QVVGIDNLNDYYDVSLKQA 44


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3141PF00577270.040 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 27.1 bits (60), Expect = 0.040
Identities = 14/49 (28%), Positives = 22/49 (44%), Gaps = 1/49 (2%)

Query: 11 APGIDALLRRSFESDAEAKLVHDLREDGF-LTLGLVATDDEGQVVGYVA 58
P A++R F++ KL+ L + L G + T + Q G VA
Sbjct: 779 VPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVA 827


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3154TCRTETOQM732e-15 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 73.0 bits (179), Expect = 2e-15
Identities = 69/313 (22%), Positives = 110/313 (35%), Gaps = 77/313 (24%)

Query: 398 IMGHVDHGKTSLLDYI-----RSTKVASGEAG-------------GITQHIGAYHVETDN 439
++ HVD GKT+L + + T++ S + G GIT G + +N
Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67

Query: 440 GMITFLDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAV 499
+ +DTPGH F + R D +L+++A DGV QT + G+P + +
Sbjct: 68 TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFI 127

Query: 500 NKIDKPEADPDRV----KNELSQYGI-----------------LPEEWG----------- 527
NKID+ D V K +LS + E+W
Sbjct: 128 NKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLE 187

Query: 528 ---------------GESQFV---------HVSAKAGTGIDELLDAILLQAEVLELKAVR 563
ES H SAK GID L++ I +
Sbjct: 188 KYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVIT--NKFYSSTHRG 245

Query: 564 KGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVL-CGFEYGRVRAMRNELGQEVLEA 622
+ G V + + R +A + + G LH D V E ++ M + E+ +
Sbjct: 246 QSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKI 305

Query: 623 GPSIPVEILGLSG 635
+ EI+ L
Sbjct: 306 DKAYSGEIVILQN 318


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3160SECGEXPORT1587e-54 Protein-export SecG membrane protein signature.
		>SECGEXPORT#Protein-export SecG membrane protein signature.

Length = 110

Score = 158 bits (400), Expect = 7e-54
Identities = 107/109 (98%), Positives = 109/109 (100%)

Query: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTAVLA 60
MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTA+LA
Sbjct: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60

Query: 61 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAQPTSDIP 109
TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA+PTSDIP
Sbjct: 61 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIP 109


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3163HTHFIS340.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 0.002
Identities = 22/82 (26%), Positives = 31/82 (37%), Gaps = 18/82 (21%)

Query: 188 VLMVGPPGTGKTLLAKAI---AGEAKVPFFT-----ISGSDFVEMFVGV------GASRV 233
+++ G GTGK L+A+A+ PF I G GA
Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTR 222

Query: 234 RD-MFEQAKKAAPCIIFIDEID 254
FEQA+ +F+DEI
Sbjct: 223 STGRFEQAEGGT---LFLDEIG 241


47SPA3213SPA3225Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3213-1173.312554ATP/GTP-binding protein
SPA32140245.502781hypothetical protein
SPA32150255.833202serine protease
SPA3216-1265.692651serine protease
SPA32170265.385729inner membrane protein
SPA32180284.924724oxaloacetate decarboxylase subunit beta
SPA32190202.116122oxaloacetate decarboxylase subunit alpha
SPA3220-118-2.244581oxaloacetate decarboxylase subunit gamma
SPA3221016-2.738067tartrate dehydratase
SPA3222116-2.050131tartrate dehydratase
SPA3224317-2.245061transcriptional regulator
SPA3225416-2.588909GntR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3215V8PROTEASE694e-15 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 69.3 bits (169), Expect = 4e-15
Identities = 34/187 (18%), Positives = 65/187 (34%), Gaps = 32/187 (17%)

Query: 90 GLGSGVIIDAAKGYVLTNNHVINQAQKISIQL------------NDGREFDAKLIGGDDQ 137
+ SGV++ K +LTN HV++ L +G ++ +
Sbjct: 102 FIASGVVV--GKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGE 159

Query: 138 SDIALLQIQN-------PSKLTQIAIADSDKLRVGDFAVAVGNPFGLGQTATSGIISALG 190
D+A+++ + ++++ + +V G P ++ +
Sbjct: 160 GDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKP-------VATMW 212

Query: 191 RSGLNLEGLEN-FIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSIGIGFAIPSN 249
S + L+ +Q D S GNSG + N E+IGI+ I N
Sbjct: 213 ESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHW---GGVPNEFNGAVFINEN 269

Query: 250 MAQTLAQ 256
+ L Q
Sbjct: 270 VRNFLKQ 276


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3216V8PROTEASE534e-10 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 53.5 bits (128), Expect = 4e-10
Identities = 31/160 (19%), Positives = 59/160 (36%), Gaps = 26/160 (16%)

Query: 77 RTLGSGVIMDQRGYIITNKHVINDADQIIVALQ------------DGRVFEALLVGSDSL 124
+ SGV++ + ++TNKHV++ AL+ +G +
Sbjct: 101 TFIASGVVVG-KDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGE 159

Query: 125 TDLAVLKI-------NATGGLPTIPINTKRTPHIGDVVLAIGNPYNLGQTITQGIISATG 177
DLA++K + + ++ + + G P + T + G
Sbjct: 160 GDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGD-KPVATMW--ESKG 216

Query: 178 RIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINT 217
+I + +Q D S GNSG + N E++GI+
Sbjct: 217 KI---TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHW 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3219RTXTOXIND320.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.1 bits (73), Expect = 0.007
Identities = 16/58 (27%), Positives = 28/58 (48%)

Query: 507 ASAPAAAAPAGAGTPVTAPLAGNIWKVIATEGQTVAEGDVLLILEAMKMETEIRAAQA 564
A+A +G + + ++I EG++V +GDVLL L A+ E + Q+
Sbjct: 84 ATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQS 141



Score = 31.0 bits (70), Expect = 0.016
Identities = 15/56 (26%), Positives = 22/56 (39%), Gaps = 10/56 (17%)

Query: 532 KVIATEGQTVAEGDVLLILEAMKMETEIRAAQAGTVRGIAVKSGDAVSVGDPLMTL 587
V G+ G EI+ + V+ I VK G++V GD L+ L
Sbjct: 82 IVATANGKLTHSGRSK----------EIKPIENSIVKEIIVKEGESVRKGDVLLKL 127


48SPA3410SPA3442Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3410-2203.134373hypothetical protein
SPA3411-3203.282919high-affinity branched-chain amino acid
SPA3412-2182.520528high-affinity branched-chain amino acid
SPA3413-1182.301556high-affinity branched-chain amino acid
SPA34140171.386756high-affinity branched-chain amino acid
SPA3415-1181.783164leucine-specific binding protein
SPA34160202.288463hypothetical protein
SPA34172192.275066hypothetical protein
SPA34182172.552072high-affinity branched-chain amino acid
SPA34192162.078053RNA polymerase sigma-32 factor
SPA34203152.185586cell division protein
SPA34212142.043612cell division ATP-binding protein FtsE
SPA34222143.852540cell division protein
SPA34232153.885432hypothetical protein
SPA34241153.791049hypothetical protein
SPA34251143.450185hypothetical protein
SPA34261133.321035hypothetical protein
SPA34270133.703832heavy metal-transporting ATPase
SPA34280131.301227methyl-accepting chemotaxis citrate transducer
SPA34292151.490440hypothetical protein
SPA34301141.522398hypothetical protein
SPA34310152.132261lipoprotein
SPA3432-2153.214261hypothetical protein
SPA3433-1133.429254hypothetical protein
SPA3434-1112.651422hypothetical protein
SPA34351120.566655nickel responsive regulator
SPA34360130.017216ABC-transporter ATP-binding protein
SPA3437114-1.688942ABC transporter ATP-binding protein
SPA3438327-6.557631HlyD-family secretion protein
SPA3439735-9.736229hypothetical protein
SPA3440230-7.776086aminotransferase
SPA3441019-4.895486regulatory protein
SPA3442017-4.403514DNA-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3425SHIGARICIN270.026 Ribosome inactivating protein family signature.
		>SHIGARICIN#Ribosome inactivating protein family signature.

Length = 289

Score = 26.7 bits (59), Expect = 0.026
Identities = 6/29 (20%), Positives = 16/29 (55%)

Query: 7 FFIIIIALIVVAASFRFVQQRREKAANEA 35
+++I AA ++F++Q+ K ++
Sbjct: 173 ALMVLIQSTSEAARYKFIEQQIGKRVDKT 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3427ACRIFLAVINRP300.039 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 30.2 bits (68), Expect = 0.039
Identities = 17/78 (21%), Positives = 34/78 (43%), Gaps = 3/78 (3%)

Query: 336 AEERRAPIERFIDRFSRIYTPVIMVIALLVTLIPPLMFDGGWQEWIYKGLTLLLIGCPCA 395
E++ P E S+I ++ + +L + P+ F GG IY+ ++ ++ A
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVS---A 477

Query: 396 LVISTPAAITSGLAAAAR 413
+ +S A+ A A
Sbjct: 478 MALSVLVALILTPALCAT 495


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3429PF012061012e-32 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 101 bits (254), Expect = 2e-32
Identities = 28/72 (38%), Positives = 42/72 (58%)

Query: 9 DHTLDALGLRCPEPVMMVRKTVRNMQTGETLLIIADDPATTRDIPGFCTFMEHDLLAQET 68
D +LDA GL CP P++ +KT+ M GE L ++A DP + +D F H+LL Q+
Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64

Query: 69 EGLPYRYLLRKA 80
E Y + L++A
Sbjct: 65 EDGTYHFRLKRA 76


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3432TCRTETA492e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 49.1 bits (117), Expect = 2e-08
Identities = 75/399 (18%), Positives = 141/399 (35%), Gaps = 34/399 (8%)

Query: 13 LRLNLRIVSIVMFNFASYLTIGLPLAVLPGYVHD--AMGFSAFWAGLIISLQYFATLLSR 70
++ N ++ I+ + IGL + VLPG + D G++++L
Sbjct: 1 MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACA 60

Query: 71 PHAGRYADVLGPKKIVVFGLCGCFLSGLGYLLADIASAWPMISLLLLGLGRVILGI-GQS 129
P G +D G + +++ L G + + Y + A L +L +GR++ GI G +
Sbjct: 61 PVLGALSDRFGRRPVLLVSLAG---AAVDYAIMATAPF-----LWVLYIGRIVAGITGAT 112

Query: 130 FAGTGSTLWGVGVVGSLHIGRVISWNGIVTYGAMAMGAPLGVLCYAWGGLQGL--ALTVM 187
A G+ + + R + M G LG L + A +
Sbjct: 113 GAVAGAYIADITDGDER--ARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALN 170

Query: 188 GVALLAVLLALPRPSVK----ANKGKPLPFRAVLGRVWLYGMALALA-----SAGFGVIA 238
G+ L LP + P + + +A +A V A
Sbjct: 171 GLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPA 230

Query: 239 TFITLFYDAK-GWDGAAFALTLFSVAFVGT---RLLFPNGINRLGGLNVAMICFGVEIIG 294
+F + + WD ++L + + + ++ RLG M+ + G
Sbjct: 231 ALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTG 290

Query: 295 LLLVGTAAMPWMAKIGVLLTGMGFSLVFPALGVVAVKAVPPQNQGAALATYTVFMDMSLG 354
+L+ A WMA ++L + PAL + + V + QG + ++
Sbjct: 291 YILLAFATRGWMAFPIMVLLA-SGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT-S 348

Query: 355 VTGPLAGLVMTWAGVPV----IYLAAAGLVAMALLLTWR 389
+ GPL + A + ++A A L + L R
Sbjct: 349 IVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3434ENTSNTHTASED300.006 Enterobactin synthetase component D signature.
		>ENTSNTHTASED#Enterobactin synthetase component D signature.

Length = 234

Score = 29.6 bits (66), Expect = 0.006
Identities = 25/93 (26%), Positives = 43/93 (46%), Gaps = 6/93 (6%)

Query: 30 RWASWLAGRVLLSRALSPL---PEMVYGEQGKPAFSAGTPLWFNLSHSGDTIALLLSDEG 86
R A LAGR+ AL + G++ +P + G L+ ++SH T ++S +
Sbjct: 46 RKAEHLAGRIAAVHALREVGVRTVPGMGDKRQPLWPDG--LFGSISHCATTALAVISRQ- 102

Query: 87 EVGCDIEVIRPRDNWRSLANAVFSLGEHAEMEA 119
+G DIE I + LA ++ E ++A
Sbjct: 103 RIGIDIEKIMSQHTATELAPSIIDSDERQILQA 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3436ABC2TRNSPORT473e-08 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 47.2 bits (112), Expect = 3e-08
Identities = 44/171 (25%), Positives = 74/171 (43%), Gaps = 7/171 (4%)

Query: 200 REREHGTVEHLLVMPVTPFEIMMAKV-WSMGLVVLVVSGLSLMLMVKGVLGVPIEGSIPL 258
R T E +L + +I++ ++ W+ L +G + +V LG + L
Sbjct: 93 RMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAG---IGVVAAALGY-TQWLSLL 148

Query: 259 FMLGV-ALSLLATISIGIFMGTIARSMPQLGLLMILVLLPLQMLSGGSTPRESMPQAVQD 317
+ L V AL+ LA S+G+ + +A S LV+ P+ LSG P + +P Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 318 IMLTMPTTHFVSLAQAILYRGAGLSIVWPQFLTLLAIGGVFFL-IALLRFR 367
+P +H + L + I+ + + + I FFL ALLR R
Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTALLRRR 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3438RTXTOXIND779e-18 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 77.2 bits (190), Expect = 9e-18
Identities = 70/409 (17%), Positives = 135/409 (33%), Gaps = 82/409 (20%)

Query: 4 HLVWWGAGILVAVAAIAWWMLRPAGIPEGFAASNGRI--EATEVDIATKIAGRIDTILVS 61
LV + + V A +L E A +NG++ +I + I+V
Sbjct: 58 RLVAY-FIMGFLVIAFILSVLGQV---EIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 62 EGQFVRQGEVLAKMDTRV----------------LQEQRLEAI----------------- 88
EG+ VR+G+VL K+ L++ R + +
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 89 -------------------AQIKEAESAVAAARALLEQRQSEMRAAQSVVKQREAELDSV 129
Q ++ L+++++E + + + E
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 130 SKRHVRSRSLSQRGAVSVQQLDDDRAAAESARAALETAKAQVSAAKAAIEAARTSIIQ-- 187
R SL + A++ + + A L K+Q+ ++ I +A+
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 188 -----------AQTRVEAAQATERRIVADID--DSELKAPRDGRV-QYRVAEPGEVLSAG 233
QT T + S ++AP +V Q +V G V++
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 234 GRVLNMVDLSDVY-MTFFLPTEQAGLLKIGGEARLVLDAAPDLRIPATISFVASVAQFTP 292
++ +V D +T + + G + +G A + ++A P R V V
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYG---YLVGKVKNINL 410

Query: 293 KTVETHDERLKLMFRVKARIPPELLRQHLEYV--KTGLPGMAWVRLDER 339
+E D+RL L+F V I L + + +G+ A ++ R
Sbjct: 411 DAIE--DQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMR 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3439TCRTETB300.016 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 30.2 bits (68), Expect = 0.016
Identities = 19/109 (17%), Positives = 45/109 (41%), Gaps = 10/109 (9%)

Query: 226 FAAFSIFATISFYQGSSYLVPY-LSDVYGMTAEHAGIIGMIRAYVLAILIAPVVGLLADK 284
IF T++ G +VPY + DV+ ++ G + + + I+ + G+L D+
Sbjct: 263 LCGGIIFGTVA---GFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDR 319

Query: 285 VGS--AIKVMNWLFIAGVIGVAMFLVIPQDPAMVWVLIGTLMIVGSINF 331
G + + + + L + ++ I + ++G ++F
Sbjct: 320 RGPLYVLNIGVTFLSVSFLTASFLL----ETTSWFMTIIIVFVLGGLSF 364


49SPA3467SPA3472Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3467-1133.071876hypothetical protein
SPA3468-1133.2768612-dehydro-3-deoxygluconokinase
SPA3469-2143.401504zinc-protease precursor
SPA3470-2143.945862C4-dicarboxylate transport protein
SPA3471-1144.239334hypothetical protein
SPA3472-1143.999889hypothetical protein
50SPA3485SPA3503Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3485-1153.018077dipeptide transport system permease protein
SPA3486-1122.320690dipeptide transport system permease protein
SPA3487-2111.909802periplasmic dipeptide transport protein
SPA3488-1122.604373xanthine permease
SPA3489-1103.392981hypothetical protein
SPA3490-193.428304lacI-family transcriptional regulator
SPA34930112.896323*hypothetical protein
SPA3494-1122.9599783-methyladenine DNA glycosylase I, constitutive
SPA3495-1112.994341acetyltransferase
SPA3496-1112.666294biotin sulfoxide reductase
SPA34970130.608450outer membrane protein
SPA3498016-0.8881442-hydroxyacid dehydrogenase
SPA3499224-4.249385hypothetical protein
SPA3501319-1.382147cold shock protein
SPA3502418-0.423760hypothetical protein
SPA3503521-0.280956acetyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3489PF05272310.008 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 31.2 bits (70), Expect = 0.008
Identities = 14/42 (33%), Positives = 19/42 (45%)

Query: 162 VVKEVNRDGEVVWEWRAWEHLNPEDFPIHDIFDRRHWPMING 203
V RDG W+WR W+ P FP H + R ++ G
Sbjct: 194 VYSRSQRDGSEAWKWRGWDDPRPLYFPSHRAPESRTVVLVEG 235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3495SACTRNSFRASE348e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 34.1 bits (78), Expect = 8e-05
Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 5/52 (9%)

Query: 76 VAPDALRHGIGKALL----EYVQQR-FPLLSLEVYQKNQSAVNFYHALGFRI 122
VA D + G+G ALL E+ ++ F L LE N SA +FY F I
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3497OMPADOMAIN1184e-34 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 118 bits (297), Expect = 4e-34
Identities = 43/124 (34%), Positives = 64/124 (51%), Gaps = 11/124 (8%)

Query: 104 LNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEY--PKTAVNVVGYTDSTGSHDLNMRLS 161
+ ++V F+ + ATLKP G L + L +V V+GYTD GS N LS
Sbjct: 215 FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS 274

Query: 162 QQRADSVASSLITQGVDASRIRTSGMGPANPIASNSTAEGK---------AQNRRVEITL 212
++RA SV LI++G+ A +I GMG +NP+ N+ K A +RRVEI +
Sbjct: 275 ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 213 SPLQ 216
++
Sbjct: 335 KGIK 338


51SPA3523SPA3531Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3523-1203.055544hypothetical protein
SPA3524-1213.686713dicarboxylate-binding periplasmic protein
SPA3525-2214.649609L-xylulose kinase
SPA3526-2183.504782hexulose-6-phosphate synthase
SPA3527-2173.096792sugar-phosphate isomerase
SPA3528-2133.045802sugar isomerase
SPA3529-2133.299458transcriptional regulator
SPA3530-1133.473114hypothetical protein
SPA3531-1133.084419aldehyde dehydrogenase B
52SPA3561SPA3572Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3561111-3.7937672-amino-3-ketobutyrate coenzyme A ligase
SPA3562219-6.679420ADP-L-glycero-D-manno-heptose-6-epimerase
SPA3563325-8.923700ADP-heptose--LPS-heptosyltransferase II
SPA3564436-12.985432lipopolysaccharide heptosyltransferase-1
SPA3565643-15.754325O-antigen ligase
SPA3566544-15.775661lipopolysaccharide
SPA3567548-17.955854lipopolysaccharide core biosynthesis protein
SPA3568445-16.733415lipopolysaccharide core biosynthesis protein
SPA3569341-14.154612lipopolysaccharide 1,2-glucosyltransferase
SPA3570036-11.956864lipopolysaccharide 1,3-galactosyltransferase
SPA3571-128-8.674520lipopolysaccharide 1,6-galactosyltransferase
SPA3572-224-6.864310hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3562NUCEPIMERASE993e-26 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 99.5 bits (248), Expect = 3e-26
Identities = 75/348 (21%), Positives = 125/348 (35%), Gaps = 67/348 (19%)

Query: 2 IIVTGGAGFIGSNIVKALNDKGITDILVVDNLKD--------------GTKFVNLVDLNI 47
+VTG AGFIG ++ K L + G ++ +DNL D +++
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAG-HQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 48 ADYMDKEDFLIQIMSGEELGDIEAIFHEGACSSTTEWDGKYMMDNNYQYSK-------EL 100
AD + + + G E +F + +Y ++N + Y+ +
Sbjct: 62 ADR----EGMTDLF---ASGHFERVFISPHRLAV-----RYSLENPHAYADSNLTGFLNI 109

Query: 101 LHYCLEREIP-FLYASSAATYGGRTSD-FIESREYEKPLNVYGYSKFLFDEYVRQILPEA 158
L C +I LYASS++ YG F + P+++Y +K +
Sbjct: 110 LEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLY 169

Query: 159 NSQIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA 218
G R+F VYGP + MA F + G+S ++ KRDF Y+ D+A
Sbjct: 170 GLPATGLRFFTVYGPWG--RPDMA--LFKFTKAMLEGKSIDVY-NYGKMKRDFTYIDDIA 224

Query: 219 AVNL------------WFLESGKSG-------IFNLGTGRAESFQAVADATLAY-HKKGS 258
+ W +E+G ++N+G A +
Sbjct: 225 EAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAK 284

Query: 259 IEYIPFPDKLKGRYQAFTQADLTNLRNA-GYDKPFKTVAEGVTEYMAW 305
+P G T AD L G+ P TV +GV ++ W
Sbjct: 285 KNMLPLQ---PGDVL-ETSADTKALYEVIGF-TPETTVKDGVKNFVNW 327


53SPA3595SPA3608Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3595-1123.095054tRNA (guanosine-2'-O)-methyltransferase
SPA3596-1112.587274ATP-dependent DNA helicase
SPA3597-1100.911617glutamate permease
SPA3598-211-0.157039purine permease
SPA3599-113-2.953796hypothetical protein
SPA3601316-3.266469sodium:galactoside family symporter
SPA3603320-4.737830*hypothetical protein
SPA3604319-4.462059hypothetical protein
SPA3606319-3.796576DNA-binding protein
SPA3607419-3.732464hypothetical protein
SPA3608213-1.799967autotransported protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3596SECA411e-05 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 41.0 bits (96), Expect = 1e-05
Identities = 38/141 (26%), Positives = 60/141 (42%), Gaps = 18/141 (12%)

Query: 233 NLSMLALRAGAQRYHAQPLSTNNILKDKLLAALPFKPTGAQARVVAEIERDM-ALDVPMM 291
LS L+ + A+ L +L++ + A A R ++ M DV ++
Sbjct: 37 KLSDEELKGKTAEFRAR-LEKGEVLENLIPEAF------AVVREASKRVFGMRHFDVQLL 89

Query: 292 ---RLVQGDV-----GSGKTLVAALAA-LRAIAHGKQVALMAPTELLAEQHANNFRNWFE 342
L + + G GKTL A L A L A+ GK V ++ + LA++ A N R FE
Sbjct: 90 GGMVLNERCIAEMRTGEGKTLTATLPAYLNALT-GKGVHVVTVNDYLAQRDAENNRPLFE 148

Query: 343 PLGVEVGWLAGKQKGKARQAQ 363
LG+ VG A++
Sbjct: 149 FLGLTVGINLPGMPAPAKREA 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3604IGASERPTASE352e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.7 bits (79), Expect = 2e-04
Identities = 27/108 (25%), Positives = 47/108 (43%), Gaps = 3/108 (2%)

Query: 25 GYHIEHVENKSQQPGRTFDYQNLAASALDSENGLPQLGINAFGGHVQG-KNKSVDMAQFI 83
GY + S + +F+ NL + +E+ LG G +Q N V + +
Sbjct: 793 GYVTCTTDKLSDKALNSFNPTNLRGNVNLTESANFVLGKANLFGTIQSRGNSQVRLTENS 852

Query: 84 HCHLP-DCSRYFAYLSNGHV-VPSIDLTEQEAEYAQYTIDHLNLNSGF 129
H HL + + L+NGH+ + S D + +Y T++ L+ N F
Sbjct: 853 HWHLTGNSDVHQLDLANGHIHLNSADNSNNVTKYNTLTVNSLSGNGSF 900


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3608PERTACTIN1191e-29 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 119 bits (300), Expect = 1e-29
Identities = 164/749 (21%), Positives = 289/749 (38%), Gaps = 90/749 (12%)

Query: 230 TGDSSEGLRTGQSGSLIRLGDDATIETSGASSTGIYAASSSRTELGNNATITVNGASAHA 289
TG + G+ G+++ L ATI A + G + +
Sbjct: 236 TGGRAAGV-AAMDGAIVHL-QRATIRRGDAPAGGAVPGGAVPGGAVPGG-FGPLLDGWYG 292

Query: 290 VYATNATVNLGENATISVNSASKAASYSKAPAGLYALSRGAINLAGGAAITMAGDNSSES 349
V +++TV+L A V + A+ +S G+++ G I G
Sbjct: 293 VDVSDSTVDL---AQSIVEAPQLGAAIRAGRGARVTVSGGSLSAPHGNVIETGGGARRFP 349

Query: 350 YAISTETGGIVDGS--SGGRFVIDGDIRAAGATAASGTLPQ--------------QNSTI 393
S + + G+ G + T A G Q + +
Sbjct: 350 PPASPLSITLQAGARAQGRALLYRVLPEPVKLTLAGGAQGQGDIVATELPPIPGASSGPL 409

Query: 394 KLNMTDNSRWDGASYITSATAGTGVISVQMSDATWNMTSSSTLTDLTLNSGATINFSH-- 451
+ + +RW GA+ V S+ + +ATW MT +S + L L S +++F
Sbjct: 410 DVALASQARWTGATRA--------VDSLSIDNATWVMTDNSNVGALRLASDGSVDFQQPA 461

Query: 452 EDGEPWQTLTINEDYVGNGGKLVFNTVLSDDDSETDRLQVLGNTSGNTFVAVNNIGGAGA 511
E G ++ L ++ G+G +F + D +D+L V+ + SG + V N G A
Sbjct: 462 EAGR-FKVLMVDT-LAGSG---LFRMNVFADLGLSDKLVVMRDASGQHRLWVRNSGSEPA 516

Query: 512 QTIEGIEIVNVAGNSNGTFEKASR---IVAGAYDYNMVQKGKNWYLTSYIEPDEPIIPDP 568
+ + +V S TF A++ + G Y Y + G + S + P P P
Sbjct: 517 -SGNTMLLVQTPRGSAATFTLANKDGKVDIGTYRYRLAANGNGQW--SLVGAKAPPAPKP 573

Query: 569 VDPVIPDPVIPDPVDPDPVDPVIPDPVIPDPVDPEPVDPVIPDPTIPDIGQSDTPPITEH 628
P P P P P P P P P +P P P ++ + +
Sbjct: 574 APQPGPQPGPQPPQPPQPPQP----PQPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTG 629

Query: 629 QFRPEVGSYLANNYAANTLFMTRLHDRLGETQYTDMLTGEKKVTSLWMRNVGAHTRFNDG 688
+ A + A L RLGE + G W R + ++
Sbjct: 630 GVGLASTLWYAESNA--------LSKRLGELRLNPDAGG------AWGRGFAQRQQLDNR 675

Query: 689 SGQLKTRINSYVLQLGGDLAQWSTDGLDRWHIGAMAGYANSQNRTQSSVSDYHSRGQVTG 748
+G+ + +LG D A + G RWH+G +AGY + D G
Sbjct: 676 AGRRFDQ-KVAGFELGADHA-VAVAG-GRWHLGGLAGYTRGD---RGFTGD--GGGHTDS 727

Query: 749 YSVGLYGTWYANNIDRSGAYVDTWMLFNWFDN--KVMGQDQAA--EKYKSKGITASVEAG 804
VG Y T+ AN+ G Y+D + + +N KV G D A KY++ G+ S+EAG
Sbjct: 728 VHVGGYATYIANS----GFYLDATLRASRLENDFKVAGSDGYAVKGKYRTHGVGVSLEAG 783

Query: 805 YSFRLGESVHQSYWLQPKAQVVWMGVQADDNREANGTLVKDDTAGNLLTRMGVKAYINGH 864
F ++L+P+A++ V R ANG V+D+ ++L R+G++
Sbjct: 784 RRFAH----ADGWFLEPQAELAVFRVGGGAYRAANGLRVRDEGGSSVLGRLGLEV----G 835

Query: 865 NAIDDNKSREFQPFVEANWIHNTQPA-SVKMDDVS--SDMRGTKNIGELKVGIEGQITPR 921
I+ R+ QP+++A+ + A +V+ + ++ +++RGT+ EL +G+ +
Sbjct: 836 KRIELAGGRQVQPYIKASVLQEFDGAGTVRTNGIAHRTELRGTR--AELGLGMAAALGRG 893

Query: 922 LNVWGNVAQQVGDQGYSNTQGLLGVKYSF 950
+++ + G + G +YS+
Sbjct: 894 HSLYASYEYSKGPKLAMPWTFHAGYRYSW 922


54SPA3626SPA3633Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3626224-4.427459inner membrane transport protein
SPA3628533-7.079493DNA-binding protein
SPA3629635-7.426220PTS system phosphocarrier protein
SPA3630635-7.862563hypothetical protein
SPA3631631-6.891511carbohydrate kinase
SPA3632327-6.163695PTS system transporter subunit IIC
SPA3633020-3.278292PTS system transporter subunit IIB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3626TCRTETA362e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 36.0 bits (83), Expect = 2e-04
Identities = 40/208 (19%), Positives = 77/208 (37%), Gaps = 13/208 (6%)

Query: 33 ITVEFLPVSLLTP----MAQDLGISEGVAGQSVTVTAFVAMFSSLFITQIIQATDR--RY 86
+ ++ + + L+ P + +DL S V + A A+ + +DR R
Sbjct: 14 VALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRR 73

Query: 87 IVILFAVLLTA-SCLMVSFANSFTLLLLGRACLGLALGGFWAISASLTMRLVPARTVPKA 145
V+L ++ A +++ A +L +GR G+ G A++ + + +
Sbjct: 74 PVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDERARH 132

Query: 146 LSVIFGAVSIALVIAAPLGSFLGGIIGWRNVFNAAAVMGVLCVIWVVKSLP-SLPGEPSH 204
+ +V LG +GG F AAA + L + LP S GE
Sbjct: 133 FGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRP 191

Query: 205 QKQ---NMFSLLQRPGVMAGMIAIFMSF 229
++ N + + M + A+ F
Sbjct: 192 LRREALNPLASFRWARGMTVVAALMAVF 219


55SPA3658SPA3667Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA36581337.544396heat shock protein B
SPA36593388.796417heat shock protein A
SPA366044010.164714lipoprotein
SPA366154311.109172ATP/GTP-binding protein
SPA366375313.631639heme lyase/disulfide oxidoreductase, cytochrome
SPA366485615.027966cytochrome c-type biogenesis protein F1
SPA36651318.316365cytochrome c-type biogenesis protein E1
SPA36663245.774291heme exporter protein D1
SPA36670184.171987heme exporter protein C1
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3665PF04335290.006 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 29.0 bits (65), Expect = 0.006
Identities = 10/30 (33%), Positives = 12/30 (40%)

Query: 1 MNLRRKNRLWVVCAVLAGLALTTALVLYAL 30
R K WVV V LA + + AL
Sbjct: 27 AAERSKKLAWVVAGVAGALATAGVVAVAAL 56


56SPA3701SPA3710Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA37012290.547391UDP-N-acetylglucosamine pyrophosphorylase
SPA37024320.625038hypothetical protein
SPA37036370.952838ATP synthase subunit epsilon
SPA37046390.829031ATP synthase subunit beta
SPA37056320.051457ATP synthase subunit gamma
SPA3706633-0.099415ATP synthase subunit alpha
SPA3707420-1.275057ATP synthase subunit delta
SPA37083200.029663ATP synthase subunit B
SPA37092170.016373ATP synthase subunit C
SPA3710213-0.328223ATP synthase subunit A
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3708PYOCINKILLER270.043 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 26.7 bits (58), Expect = 0.043
Identities = 15/42 (35%), Positives = 21/42 (50%)

Query: 70 AEAQVIIEQANKRRAQILDEAKTEAEQERTKIVAQAQAEIEA 111
A+A + ANK R Q EAK +AE++ + A A A
Sbjct: 210 AKASIEAAAANKAREQAAAEAKRKAEEQARQQAAIRAANTYA 251


57SPA3769SPA3793Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3769-1133.088885UDP-N-acetyl-D-mannosaminuronic acid
SPA3770-1122.506254amino acid permease
SPA3776-1122.413680****Porphyrin biosynthetic protein
SPA3777-1110.361529uroporphyrinogen III methylase
SPA3778-112-0.794161uroporphyrinogen III synthase
SPA3779013-1.390080porphobilinogen deaminase
SPA3780014-2.737746adenylate cyclase
SPA3781128-7.079157hypothetical protein
SPA3782-219-4.358636hypothetical protein
SPA3783-2140.511407hypothetical protein
SPA3784-1162.444471CyaY protein
SPA3785-1152.417646hypothetical protein
SPA3787-1163.702750lipoprotein
SPA37880173.331326diaminopimelate epimerase
SPA37890152.004182hypothetical protein
SPA3790-1140.573231integrase/recombinase
SPA3791-2140.056099hypothetical protein
SPA3792-2150.075761DNA helicase II
SPA3793013-3.375437magnesium and cobalt transport protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3769ADHESNFAMILY280.028 Adhesin family signature.
		>ADHESNFAMILY#Adhesin family signature.

Length = 309

Score = 28.3 bits (63), Expect = 0.028
Identities = 18/90 (20%), Positives = 32/90 (35%), Gaps = 9/90 (10%)

Query: 24 SH-ALNYLFADGQLKQGTLVAINAEKLLTAEDNPEVRALIGAAEFKYADGISVVRSIRKK 82
S A Y + + IN E+ T E + + + + V S+ +
Sbjct: 204 SEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLVEKLRQTKVP---SLFVESSVDDR 260

Query: 83 FPQAQVSRVAGADLWEAL----MARAGKEG 108
P VS+ ++ + +A GKEG
Sbjct: 261 -PMKTVSQDTNIPIYAQIFTDSIAEQGKEG 289


58SPA3849SPA3855Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3849-114-3.997706hypothetical protein
SPA3850-213-3.986960GTP-binding protein
SPA3851121-5.732409hypothetical protein
SPA3852220-6.684145hypothetical protein
SPA3853119-7.026146hypothetical protein
SPA3854115-5.797309hypothetical protein
SPA3855-113-3.326717hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3850TCRTETOQM1797e-51 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 179 bits (456), Expect = 7e-51
Identities = 100/448 (22%), Positives = 170/448 (37%), Gaps = 87/448 (19%)

Query: 4 NLRNIAIIAHVDHGKTTLVDKLLQQSGTFDARAETQE--RVMDSNDLEKERGITILAKNT 61
+ NI ++AHVD GKTTL + LL SG + D+ LE++RGITI T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 62 AIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAFDGPMPQTRFVTKKAFAHGL 121
+ +W + ++NI+DTPGH DF EV R +S++D +L++ A DG QTR + G+
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 122 KPIVVINKVDRPGARPDWVVDQVFD-------------LFVNLDATDEQLD--------- 159
I INK+D+ G V + + L+ N+ T+
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 160 --------------------------------FPIIYASALNGIAGLDHEDMAEDMTPLY 187
FP+ + SA N I G+D+ L
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNI-GIDN---------LI 231

Query: 188 QAIVDHVPAPDVDLDGPLQMQISQLDYNNYVGVIGIGRIKRGKVKPNQQVTIIDSEGKTR 247
+ I + + L ++ +++Y+ + R+ G + V I + E
Sbjct: 232 EVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-- 289

Query: 248 NAKVGKVLTHLGLERIDSDIAEAGDIIAITGLG-ELN--ISDTICDPQNVEALPALSVDE 304
K+ ++ T + E D A +G+I+ + +LN + DT PQ +
Sbjct: 290 --KITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQR----ERIENPL 343

Query: 305 PTVSMFFCVNTSPFCGKEGKFVTSRQILDRLNKELVHNVALRVEETEDADAFRVSGRGEL 364
P + + + D L LR +S G++
Sbjct: 344 PLLQTTVEPSKPQQREMLLDALLEISDSDPL---------LRYYVDSATHEIILSFLGKV 394

Query: 365 HLSVLIENMRRE-GFELAVSRPKVIFRE 391
+ V ++ + E+ + P VI+ E
Sbjct: 395 QMEVTCALLQEKYHVEIEIKEPTVIYME 422



Score = 32.5 bits (74), Expect = 0.005
Identities = 13/75 (17%), Positives = 29/75 (38%), Gaps = 1/75 (1%)

Query: 398 EPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIGFRSEFMT 457
EPY + + +++ + ++ + V L IP+R + +RS+
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKN-NEVILSGEIPARCIQEYRSDLTF 595

Query: 458 MTSGTGLLYSTFSHY 472
T+G + + Y
Sbjct: 596 FTNGRSVCLTELKGY 610


59SPA3914SPA3920Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3914219-1.503760hypothetical protein
SPA3915118-1.496639sugar kinase
SPA3916320-2.912017regulatory protein
SPA3917221-3.345249ABC transporter ATP-binding protein
SPA3918423-3.610631ABC transporter permease
SPA3919121-3.435981ABC-transporter membrane protein
SPA3920-118-3.052763ABC transporter substrate-binding protein
60SPA4069SPA4097Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4069-113-3.265096hypothetical protein
SPA4070-117-6.099412lipoprotein
SPA4071120-8.358079excision nuclease subunit A
SPA4072330-8.496799single-strand DNA-binding protein
SPA4073432-9.857312hypothetical protein
SPA4074331-9.424208hypothetical protein
SPA4075330-8.665947type-I secretion protein
SPA4076329-8.004804type-I secretion protein
SPA4077327-7.008555inner membrane protein
SPA4078-126-8.498503type-1 secretion protein
SPA4079-214-0.822988hypothetical protein
SPA4080-2140.066011hypothetical protein
SPA4081-2132.230249regulatory protein SoxS
SPA4082-3132.406412SoxR protein
SPA4084-3132.883147glutathione-S-transferase
SPA4085-3163.051244xanthine/uracil permeases family protein
SPA4086-2172.920112sodium/hydrogen exchanger family protein
SPA4087-2162.390362lysR family regulatory protein
SPA4088-2191.912398hypothetical protein
SPA4089-1181.856545hypothetical protein
SPA4090-1171.653109Sodium:solute symporter family protein
SPA4092-1161.919531acetyl-coenzyme A synthetase
SPA40930182.949976hypothetical protein
SPA40940173.488249cytochrome c552 precursor
SPA40952184.337454cytochrome c-type protein NrfB precursor
SPA40961193.800067cytochrome c-type biogenesis protein
SPA40972183.245841cytochrome c-type biogenesis protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4073HTHFIS290.014 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.014
Identities = 12/62 (19%), Positives = 24/62 (38%), Gaps = 14/62 (22%)

Query: 134 AWLEDKTNSNLLIEMVIPQADISFSDSLRLGYERGIILMKEIKKIYPDV-VIDMSVNSAA 192
W+ ++ ++V+P + L+ IKK PD+ V+ MS +
Sbjct: 41 RWIAAGDGDLVVTDVVMPDEN-------------AFDLLPRIKKARPDLPVLVMSAQNTF 87

Query: 193 SS 194
+
Sbjct: 88 MT 89


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4076RTXTOXIND2668e-87 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 266 bits (682), Expect = 8e-87
Identities = 87/425 (20%), Positives = 175/425 (41%), Gaps = 25/425 (5%)

Query: 9 LMMIIISLTILIIILTYFIEINSVVHGQGVITTKDNAQLISLSKGGTIQDIYVAEGDTVK 68
+ I+ ++ IL+ ++ V G +T ++ I + +++I V EG++V+
Sbjct: 60 VAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVR 119

Query: 69 KGELLAKVVNLDLQKEYQRYRTQKGYLDKDVNEI-------SFILDKENESGLITLDGTR 121
KG++L K+ L E +TQ L + + S L+K E L +
Sbjct: 120 KGDVLLKLT--ALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQ 177

Query: 122 SLSNKEVKANIELVHSQIRA-------KELKKTSLDSEISGLQEKLSSKEKELALLAEEI 174
++S +EV L+ Q KEL +E + +++ E + +
Sbjct: 178 NVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRL 237

Query: 175 NILSPLVKKGISPYTNFLNKKQAYIKVKSEINDIESSITLKKDDIELVVNDIEALNNELR 234
+ S L+ K L ++ Y++ +E+ +S + + +I + + + +
Sbjct: 238 DDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFK 297

Query: 235 LSLSKIISKNLQELEVVNSTLKVIEKQINEEDIYSPVDGVIYKINKSATTHGGVIQAADL 294
+ + + + ++ L E++ I +PV + ++ T GGV+ A+
Sbjct: 298 NEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQL--KVHTEGGVVTTAET 355

Query: 295 LFEIKPKVRTMLADVKILPKYRDQIYVDEAVKLDVQSIIQPKIKSYNATIDNISPDSYEE 354
L I P+ T+ + K I V + + V++ + + NI+ D+ E+
Sbjct: 356 LMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIED 415

Query: 355 NTGGTIQRYYKVIIAFDVNE----DDLRWLKPGMTVDASVITGKHSIMEYLLSPLMKGVD 410
G + VII+ + N + L GM V A + TG S++ YLLSPL + V
Sbjct: 416 QRLGL---VFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMRSVISYLLSPLEESVT 472

Query: 411 KAFSE 415
++ E
Sbjct: 473 ESLRE 477


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4077GPOSANCHOR494e-07 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 49.3 bits (117), Expect = 4e-07
Identities = 49/190 (25%), Positives = 76/190 (40%), Gaps = 30/190 (15%)

Query: 96 DSAQVEKKGNGKRRNKKEEEELKKQLDEAENAKK--EADKAK-EEAEKAKEAAEKALNEA 152
A+ + + + L++ LD + AKK EA+ K EE K EA+ ++L
Sbjct: 293 LEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRD 352

Query: 153 FEVQNSSK-QIEEMLQNFLADNVAKDNLAQQSDASQQNTQA---KATQASKQNDAEKVLP 208
+ +K Q+E Q N + S+AS+Q+ + + +A KQ +
Sbjct: 353 LDASREAKKQLEAEHQKLEEQN-------KISEASRQSLRRDLDASREAKKQVEKALEEA 405

Query: 209 QPI-------NKNTSTGK--SNSSKNEEN-KLDAESVKEPLKVTLALAAES----NSGSK 254
NK K + K E KL+AE+ + LK LA AE +G
Sbjct: 406 NSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEA--KALKEKLAKQAEELAKLRAGKA 463

Query: 255 DDSITNFTKP 264
DS T KP
Sbjct: 464 SDSQTPDAKP 473



Score = 47.4 bits (112), Expect = 1e-06
Identities = 35/136 (25%), Positives = 63/136 (46%), Gaps = 4/136 (2%)

Query: 98 AQVEKKGNGKRRNKKEEEELKKQLDEAENAKKEADKAKEEAEKAKEAAEKALNEAFEVQN 157
A+ +K + ++ + L++ LD + AKK+ +KA EEA A EK E E +
Sbjct: 365 AEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKK 424

Query: 158 SSKQIEEMLQNFL-ADNVA-KDNLAQQSD--ASQQNTQAKATQASKQNDAEKVLPQPINK 213
+++ + LQ L A+ A K+ LA+Q++ A + +A +Q K +P
Sbjct: 425 LTEKEKAELQAKLEAEAKALKEKLAKQAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQA 484

Query: 214 NTSTGKSNSSKNEENK 229
+ K N +K +
Sbjct: 485 PQAGTKPNQNKAPMKE 500



Score = 43.1 bits (101), Expect = 2e-05
Identities = 17/115 (14%), Positives = 41/115 (35%), Gaps = 19/115 (16%)

Query: 101 EKKGNGKRRNKKEEEELKKQLDEAENAKKEAD-------KAKEEAEKAKEAAEKALNEAF 153
++ ++ + + + + E + + + A ++L
Sbjct: 260 ARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQ-SQVLNANRQSLRRDL 318

Query: 154 EVQNSSK-QIEEMLQNFLADNVAKDNLAQQSDASQQNTQAK---ATQASKQNDAE 204
+ +K Q+E Q N + S+AS+Q+ + + +A KQ +AE
Sbjct: 319 DASREAKKQLEAEHQKLEEQN-------KISEASRQSLRRDLDASREAKKQLEAE 366


61SPA4125SPA4138Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4125216-0.951856anaerobic dimethyl sulfoxide reductase subunit
SPA4126220-4.284784hypothetical protein
SPA4127224-7.889830hypothetical protein
SPA4128636-11.854363hypothetical protein
SPA4129440-12.437040hypothetical protein
SPA4130337-10.734668hypothetical protein
SPA4131439-10.446656GerE family regulatory protein
SPA4132335-7.754990araC family regulatory protein
SPA4133018-0.737405hypothetical protein
SPA4134017-0.038733hypothetical protein
SPA41351130.202750acetyltransferase
SPA4136218-0.633709nonspecific acid phosphatase
SPA41382190.189446*transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4135SACTRNSFRASE300.003 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 29.9 bits (67), Expect = 0.003
Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 6/65 (9%)

Query: 89 LAVDKSLHGQGVARALVRDAGLRVIQVAETIGIRGMLVHALSDE--AREFYQRVGFVPSP 146
+AV K +GV AL+ A I+ A+ G+++ A FY + F+
Sbjct: 95 IAVAKDYRKKGVGTALLHKA----IEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150

Query: 147 MDPMM 151
+D M+
Sbjct: 151 VDTML 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4138HTHTETR461e-08 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 46.2 bits (109), Expect = 1e-08
Identities = 29/189 (15%), Positives = 53/189 (28%), Gaps = 15/189 (7%)

Query: 3 REDILGEALKLLETQGIADTTLEMVAERVNRPLDTLQRFWPDKEAILYDALRYLSQQVDI 62
R+ IL AL+L QG++ T+L +A+ + + DK + + +
Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72

Query: 63 WRRQLLLDESLSAEQKLLARYSA-LSECVSNNRYPGCLFIAACTFYPDPTH----PIHQL 117
+ L L V+ R + F+ + Q
Sbjct: 73 LELEYQAKFPGDPLSVLREILIHVLESTVTEERRRL---LMEIIFHKCEFVGEMAVVQQA 129

Query: 118 ANQQKRAAHDFTHELLTTL----EID---DPAMVARQMELVLEGCLSRMLVNRSQADVDT 170
++D + L + A M + G + L D+
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKK 189

Query: 171 AQRLAEDIL 179
R IL
Sbjct: 190 EARDYVAIL 198


62SPA4168SPA4185Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4168-1113.105618arginine-binding periplasmic protein
SPA4172-1133.561248***4Fe-4S binding protein
SPA4173-1143.624262hypothetical protein
SPA4174-3162.551511hypothetical protein
SPA4175-2132.923577N-acetylmuramoyl-L-alanine amidase
SPA41760162.273677DNA mismatch repair protein
SPA41771181.204986tRNA delta-2-isopentenylpyrophosphate (IPP)
SPA41784231.028415host factor-I protein
SPA41794210.888767HflX protein, putative GTP-binding protein
SPA41803201.172013HflK protein
SPA41814180.990706HflC protein
SPA4182216-0.061867hypothetical protein
SPA41832140.070866adenylosuccinate synthetase
SPA4184112-0.108152hypothetical protein
SPA4185211-0.076499ribonuclease R
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4175PF03544310.007 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 31.1 bits (70), Expect = 0.007
Identities = 16/65 (24%), Positives = 26/65 (40%), Gaps = 7/65 (10%)

Query: 130 PPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASAGAGDKVVI 189
P P P P K+VE R +P + S + + RP + + A K V
Sbjct: 99 PKPKPKPKPVKKVEQPKRDVKPVESRPASPFE-------NTAPARPTSSTATAATSKPVT 151

Query: 190 AIDAG 194
++ +G
Sbjct: 152 SVASG 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4176ALARACEMASE300.027 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 30.1 bits (68), Expect = 0.027
Identities = 26/161 (16%), Positives = 57/161 (35%), Gaps = 18/161 (11%)

Query: 31 VENSLDAGATRVDIDIER---GGAKLIR-IRDNGCGIKKEELALALARHATSKIASLDDL 86
++ SLD A + ++ I R A++ ++ N G E + A+ + +L++
Sbjct: 5 IQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLNLEEA 64

Query: 87 EAIISLGFRGEAL----------ASISSVSRLTLTSRTAEQAEAWQAYAEGRDMDVTVK- 135
+ G++G L I RLT + Q +A Q +D+ +K
Sbjct: 65 ITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKV 124

Query: 136 -PAAHPVGTTLEVLDLFYNTPARRKFMRTEK--TEFNHIDE 173
+ +G + + + + + F +
Sbjct: 125 NSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEH 165


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4179SECA330.002 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 33.3 bits (76), Expect = 0.002
Identities = 26/144 (18%), Positives = 55/144 (38%), Gaps = 6/144 (4%)

Query: 282 HVVDAADVRVQENIEAVNTVLEEIDAHEIPTLMVMNKIDMLDDFEPRIDRDEENK-PIRV 340
++D +DV N + IDA+ P + ++ + + R+ D + PI
Sbjct: 665 ELLDVSDVSETINSIREDVFKATIDAYIPPQSL--EEMWDIPGLQERLKNDFDLDLPIAE 722

Query: 341 WLSAQSGVGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKEWMEEDGSV 400
WL + + L + + + + + + R + LQ ++ W E ++
Sbjct: 723 WLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAM 782

Query: 401 SLQVRMPIVDWRRLCKQEPALIEY 424
+R I R +++P EY
Sbjct: 783 D-YLRQGIH-LRGYAQKDP-KQEY 803


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4181PYOCINKILLER290.030 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 29.0 bits (64), Expect = 0.030
Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 3/65 (4%)

Query: 225 NRMRAEREAVARRHRSQGQEEAEKLRAAADYEVTK---TLAEAERQGRIMRGEGDAEAAK 281
N+ R + A A+R + + +RAA Y + +A A +G I +G A A+
Sbjct: 220 NKAREQAAAEAKRKAEEQARQQAAIRAANTYAMPANGSVVATAAGRGLIQVAQGAASLAQ 279

Query: 282 LFADA 286
+DA
Sbjct: 280 AISDA 284


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4185RTXTOXIND320.013 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.7 bits (72), Expect = 0.013
Identities = 12/55 (21%), Positives = 26/55 (47%), Gaps = 1/55 (1%)

Query: 165 VVPDDSRLSFDILIPPEDVMGARMGFVVVVELTQRPTRRTKAV-GKIVEVLGDNM 218
+VP+D L L+ +D+ +G ++++ P R + GK+ + D +
Sbjct: 359 IVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAI 413


63SPA4283SPA4323Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4283-122-5.0933735-keto-D-gluconate-5-reductase
SPA4284025-6.022041L-idonate 5-dehydrogenase
SPA4285133-8.006564D-gluconate kinase, thermosensitive
SPA4286229-7.173837alcohol dehydrogenase
SPA4288335-8.814663*integrase
SPA4289437-8.426183DNA methyltransferase SptAIM; protects DNA
SPA4290530-3.949640subunit S of type I restriction-modification
SPA4291228-1.708855subunit R of type I restriction-modification
SPA42921244.405478hypothetical protein
SPA42932244.347749hypothetical protein
SPA42962253.932707phage immunity repressor protein
SPA42974242.924796hypothetical protein
SPA4298224-2.140640hypothetical P4 phage protein
SPA4299437-8.765527hypothetical protein
SPA43001066-17.964618hypothetical protein
SPA43011061-16.483473hypothetical protein
SPA4304854-14.430030fimbrial structural protein
SPA4305744-12.116853fimbrial chaperone protein
SPA4306739-9.729939outer membrane fimbrial usher protein
SPA4307428-1.897425fimbrial regulator
SPA4309417-0.101478GerE-family regulatory protein
SPA4310419-2.799180hypothetical protein
SPA4311419-3.079108outer membrane protein
SPA4312319-3.687754hypothetical protein
SPA4313221-4.379681hypothetical protein
SPA4314220-3.878167hypothetical protein
SPA4315229-5.796284hypothetical protein
SPA4316020-2.843436hypothetical protein
SPA4317024-5.589349hypothetical protein
SPA4319027-6.160203hypothetical protein
SPA4320023-3.836767hypothetical protein
SPA4321125-5.114708hypothetical protein
SPA4322122-4.387935hypothetical protein
SPA4323121-4.806828hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4283DHBDHDRGNASE1401e-42 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 140 bits (353), Expect = 1e-42
Identities = 85/256 (33%), Positives = 133/256 (51%), Gaps = 8/256 (3%)

Query: 7 LAGKNILITGAAQGIGYLLATGLGRYGARIIVNDITPERAETAVTKLQQEGIKAIAAPFN 66
+ GK ITGAAQGIG +A L GA I D PE+ E V+ L+ E A A P +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 67 VTHKQDIEAAVEHIEKDIGVIDVLINNAGIQRRHPFTEFPEQEWNDVIAVNQTAVFLVSQ 126
V I+ IE+++G ID+L+N AG+ R ++EW +VN T VF S+
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 127 AVTRRMVARQAGKVINICSMQSELGRDTITPYAASKGAVKMLTRGMCVELARHNIQVNGI 186
+V++ M+ R++G ++ + S + + R ++ YA+SK A M T+ + +ELA +NI+ N +
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 187 APGYFKTEMTKALVEDE--------AFTSWLCKRTPAARWGDPQELIGAAVFLSSKASDF 238
+PG +T+M +L DE P + P ++ A +FL S +
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 239 VNGHLLFVDGGMLVAV 254
+ H L VDGG + V
Sbjct: 246 ITMHNLCVDGGATLGV 261


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4296ICENUCLEATIN260.046 Ice nucleation protein signature.
		>ICENUCLEATIN#Ice nucleation protein signature.

Length = 1258

Score = 26.3 bits (57), Expect = 0.046
Identities = 17/60 (28%), Positives = 25/60 (41%), Gaps = 1/60 (1%)

Query: 14 MVAQAGASHEAPVSNVAGYANPVWATTSEIGVSSGSSHMQTLEVATMATTLT-TSHSQFV 72
M A G+ V + PV S + QT+E+AT +TL+ T SQ +
Sbjct: 118 MQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQTIEIATYGSTLSGTHQSQLI 177


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4299PF05775300.015 Enterobacteria AfaD invasin protein
		>PF05775#Enterobacteria AfaD invasin protein

Length = 142

Score = 30.3 bits (68), Expect = 0.015
Identities = 12/41 (29%), Positives = 18/41 (43%)

Query: 174 VNVQLINSDGLKRTLKDGAVKGTCHIIGGQKQAGKRLWIAE 214
++ L+N + L DG T II +G R+WI
Sbjct: 24 ADITLMNHKYMGNLLHDGVKLATGRIICQDTHSGFRVWINA 64


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4306PF005777240.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 724 bits (1870), Expect = 0.0
Identities = 261/859 (30%), Positives = 422/859 (49%), Gaps = 58/859 (6%)

Query: 6 ITLFVLTSVFHSGNVFSRQYNFDYGSLSLPPGENASFLSVE----TLPGNYVVDVYLNNQ 61
+ LFV + + S + F+ L+ P A E PG Y VD+YLNN
Sbjct: 28 VRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNG 87

Query: 62 LKETTELYFKS--MTQTLEPCLTKEKLIKYGIAIQELHGLQF-DNEQCVLLEHSP--LKY 116
T ++ F + Q + PCLT+ +L G+ + G+ ++ CV L
Sbjct: 88 YMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATA 147

Query: 117 TYNAANQSLLLNAPSKILSPIDSEIADENIWDDGINAFLLNYRANYLHS--KVGGE-DSY 173
+ Q L L P +S +WD GINA LLNY + ++GG
Sbjct: 148 QLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYA 207

Query: 174 FGQIQPGFNFGPWRLRNLSSW------QNLSSEKKFESAYIYAERGLKKIKSKLTVGDKY 227
+ +Q G N G WRLR+ ++W + S+ K++ + ER + ++S+LT+GD Y
Sbjct: 208 YLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGY 267

Query: 228 TSADLFDSVPFRGFSLNKDESMIPFSQRIYYPTIRGIAKTNATVEVRQNGYLIYSTSVPP 287
T D+FD + FRG L D++M+P SQR + P I GIA+ A V ++QNGY IY+++VPP
Sbjct: 268 TQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPP 327

Query: 288 GQFEIGREQIADLGVGVGVLDVSIYEKNGQVQNYTVPYSTPVLSLPDGYSKYSVTIGRYR 347
G F I A G L V+I E +G Q +TVPYS+ L +G+++YS+T G YR
Sbjct: 328 GPFTINDIYAAG---NSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYR 384

Query: 348 EVNNDYIDPVFFEGTYIYGLPYGFTLFGGVQWANIYNSYAIGASKDIGEYGALSFDWKTS 407
N P FF+ T ++GLP G+T++GG Q A+ Y ++ G K++G GALS D +
Sbjct: 385 SGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQA 444

Query: 408 VSKT-DTSNENGHAYGIRYNKNIAQTNTEVSLASHYYYSKNYRTFSEAIHSSEHDEF--- 463
S D S +G + YNK++ ++ T + L + Y + Y F++ +S +
Sbjct: 445 NSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIET 504

Query: 464 ----------------YDKNKKSTTSMLLSQALGSLGSVNLSYNYDKYWKHEGK-KSIIA 506
NK+ + ++Q LG ++ LS ++ YW + A
Sbjct: 505 QDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQA 564

Query: 507 SYGKNLNGVSLSLSYTKSTSKISEENEDLFSFLLSVPLQKLTNHE-------MYATYQNS 559
++ +LSY+ + + + + + + +++P + A+Y S
Sbjct: 565 GLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS 624

Query: 560 SSSKHDMNHDLGITGVAF-DSQLTWQARGQIE--DKSKNQKATFLNASWRGTYGEIGANY 616
M + G+ G D+ L++ + + + ++RG YG Y
Sbjct: 625 HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGY 684

Query: 617 SHNEINRDIGMNVSGGVIAHSSGITFGQSISDTAALVEAKGVSGAKVLGLPGVRTDFRGY 676
SH++ + + VSGGV+AH++G+T GQ ++DT LV+A G AKV GVRTD+RGY
Sbjct: 685 SHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGY 744

Query: 677 TISSYLTPYMNNFISIDPTTLPINTDIRQTDIQVVPTEGAIVKAVYKTSVGTNALIRITR 736
+ Y T Y N +++D TL N D+ VVPT GAIV+A +K VG L+ +T
Sbjct: 745 AVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTH 804

Query: 737 TNGKPLALGTVLSLKNNDGVIQSTSIVGEDGQAYVSGLSGVQKLIASWGNKPSDTCTVFY 796
N KPL G +++ +++ QS+ IV ++GQ Y+SG+ K+ WG + + C Y
Sbjct: 805 -NNKPLPFGAMVTSESS----QSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANY 859

Query: 797 SLPDKNKGQ-ISFLNGVCK 814
LP +++ Q ++ L+ C+
Sbjct: 860 QLPPESQQQLLTQLSAECR 878


64SPA0047SPA0059N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0047-2120.408503isoleucyl-tRNA synthetase
SPA0048-312-0.929920lipoprotein signal peptidase
SPA0049-211-2.040805FkbB-type 16 kD peptidyl-prolyl cis-trans
SPA0050-1110.196092LytB protein
SPA00510182.295639hypothetical protein
SPA00520253.738715nucleoside hydrolase
SPA0053-1242.495726transcriptional regulatory protein citb
SPA0054-1232.928651transcriptional regulator
SPA00550275.129995oxaloacetate decarboxylase subunit beta
SPA0056-2193.243005oxaloacetate decarboxylase subunit alpha
SPA0057-1130.253352oxaloacetate decarboxylase subunit gamma
SPA0058-1110.408433citrate-sodium symporter
SPA00590101.704970[citrate (pro-3S)-lyase] ligase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0047LIPPROTEIN48310.030 Mycoplasma P48 major surface lipoprotein signature.
		>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature.

Length = 428

Score = 30.7 bits (69), Expect = 0.030
Identities = 13/61 (21%), Positives = 28/61 (45%), Gaps = 5/61 (8%)

Query: 773 ADEIWGYLLGEREKYVFTGEWYDGLFGLEENEEFNDAFWDDVRYIK---DQINKELENQK 829
AD+ W + ++EK++ E + EE + N+ + ++ K + K + + K
Sbjct: 344 ADKKWSHFGTQKEKWIGVAE--NHFSNTEEQAKINNKIKEAIKMFKELPEDFVKYINSDK 401

Query: 830 A 830
A
Sbjct: 402 A 402


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0049INFPOTNTIATR290.007 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 28.8 bits (64), Expect = 0.007
Identities = 12/32 (37%), Positives = 19/32 (59%)

Query: 8 NSAILVHFTLKLDDGSTAESTRNNGKPALFRL 39
+ + V +T L DG+ +ST GKPA F++
Sbjct: 144 SDTVTVEYTGTLIDGTVFDSTEKAGKPATFQV 175


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0053HTHFIS697e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 69.1 bits (169), Expect = 7e-16
Identities = 29/141 (20%), Positives = 48/141 (34%), Gaps = 2/141 (1%)

Query: 1 MDSITTLIVEDEPMLAEILVDTIKLFPQFSIVGIADKLESAKKQIRLYQPQLILLDNFLP 60
M T L+ +D+ + +L L V I + + I L++ D +P
Sbjct: 1 MTGATILVADDDAAIRTVLNQA--LSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 DGKGIDLIRHTISTNYTGRIIFITADNHMDTISDALRMGVFDYLIKPVHYQRLQHTLERF 120
D DL+ ++ ++A N T A G +DYL KP L + R
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 121 TRYRSSLRSSEQANQTHVDAL 141
S + + L
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0054CARBMTKINASE300.017 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 30.2 bits (68), Expect = 0.017
Identities = 19/81 (23%), Positives = 30/81 (37%), Gaps = 13/81 (16%)

Query: 91 DATYITVGNEKGQRLYHVNPDEIGKYMEGGDSDDALYNAKSYVSVRKGSLGSSLRGKSPI 150
+ + G EK Q L V +E+ KY E G + GS+G +
Sbjct: 238 NGAALYYGTEKEQWLREVKVEELRKYYEEG-------------HFKAGSMGPKVLAAIRF 284

Query: 151 QDSTGKVIGIVSVGYTLEQLE 171
+ G+ I + +E LE
Sbjct: 285 IEWGGERAIIAHLEKAVEALE 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0056RTXTOXIND320.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.7 bits (72), Expect = 0.007
Identities = 16/58 (27%), Positives = 28/58 (48%)

Query: 507 ASAPAAAAPAGAGTPVTAPLAGNIWKVIATEGQTVAEGDVLLILEAMKMETEIRAAQA 564
A+A +G + + ++I EG++V +GDVLL L A+ E + Q+
Sbjct: 84 ATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQS 141



Score = 29.8 bits (67), Expect = 0.034
Identities = 14/56 (25%), Positives = 21/56 (37%), Gaps = 10/56 (17%)

Query: 532 KVIATEGQTVAEGDVLLILEAMKMETEIRAAQAGTVRGIAVKSGDAVSVGAPLMTL 587
V G+ G EI+ + V+ I VK G++V G L+ L
Sbjct: 82 IVATANGKLTHSGRSK----------EIKPIENSIVKEIIVKEGESVRKGDVLLKL 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0059LPSBIOSNTHSS403e-06 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 40.2 bits (94), Expect = 3e-06
Identities = 21/102 (20%), Positives = 42/102 (41%), Gaps = 4/102 (3%)

Query: 158 NPFTLGHRYLVEQAAAACDWLHLFVLKEDAS--FFSYTDRWALIEQGIAGIDNVTLHPGS 215
+P T GH ++E+ D +++ VL+ FS +R I + IA + N +
Sbjct: 10 DPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLPNAQVDSFE 69

Query: 216 AYMISRATFPGYFLKEKGV--VDDCHCQIDLQLFREHLAPAL 255
++ A +G+ + D ++ + + LA L
Sbjct: 70 GLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDL 111


65SPA0461SPA0472N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0461130-8.131565phosphoglycerate transporter protein
SPA0462135-9.371880phosphoglycerate transport regulatory protein
SPA0464235-10.497801phosphoglycerate transport system
SPA0465335-11.095416outer membrane protease E
SPA0467230-9.348130lipopolysaccharide modification acyltransferase
SPA0468015-3.413203bactoprenol glucosyl transferase
SPA0469-113-0.081154bactoprenol-linked glucose transferase
SPA04710100.693187*hypothetical protein
SPA0472-1101.383565VacJ lipoprotein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0461TCRTETA310.007 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 31.3 bits (71), Expect = 0.007
Identities = 40/217 (18%), Positives = 72/217 (33%), Gaps = 14/217 (6%)

Query: 28 IQALLSVFLGYLAYYIVRNNFTLSTPYLKEQLDLSATQI---GLLSSCMLIAYGISKGVM 84
I L +V L + ++ P L L S G+L + + V+
Sbjct: 8 IVILSTVALDAVGIGLI----MPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVL 63

Query: 85 SSLADKASPKVFMACGLVLCAIVNVGLGFSSAFWIFAALVVFNGLFQGMGVGPSFITIAN 144
+L+D+ + + L A+ + + W+ + G+ G IA+
Sbjct: 64 GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAG-AYIAD 122

Query: 145 WFPRRERGRVGAFWNISHNVGGGIVA-PIVGAAFAILGNEHWQSASYIVPACVAVIFALI 203
ER R F +S G G+VA P++G ++G + + A + F
Sbjct: 123 ITDGDERAR--HFGFMSACFGFGMVAGPVLG---GLMGGFSPHAPFFAAAALNGLNFLTG 177

Query: 204 VLVLGKGSPREEGLPSLEQMMPEEKVVLKTKNTAKAP 240
+L + E E + P T A
Sbjct: 178 CFLLPESHKGERRPLRREALNPLASFRWARGMTVVAA 214


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0464HTHFIS2486e-80 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 248 bits (635), Expect = 6e-80
Identities = 120/474 (25%), Positives = 191/474 (40%), Gaps = 73/474 (15%)

Query: 7 SILLIDDDVDVLDAYTQMLEQAGYRVRGFTHPFEAKEWVKADWEGIVLSDVCMPGCSGID 66
+IL+ DDD + Q L +AGY VR ++ W+ A +V++DV MP + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 67 LMTLFHQDDDQLPILLITGHGDVPMAVDAVKKGAWDFLQKPVDPGNLLILIEDALRQRRS 126
L+ + LP+L+++ A+ A +KGA+D+L KP D L+ +I AL + +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 127 VIARRQYCQQTLQVDLIGRSEWMNQFRQRLQQLAETDIAVWFYGEHGTGRMTGARYLHQL 186
++ + Q L+GRS M + + L +L +TD+ + GE GTG+ AR LH
Sbjct: 125 RPSKLEDDSQDGM-PLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY 183

Query: 187 GRNAKGPFVRYELT--PENAGQLETF-----------------IDQAQGGTLVLSHPEYL 227
G+ GPFV + P + + E F +QA+GGTL L +
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 228 TREQQHHLAR-LQSLEHRP----------FRLVGVGSASLVEQAAANQIAAELYYCFAMT 276
+ Q L R LQ E+ R+V + L + +LYY +
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 277 QIACQSLSQRPDDIEPLFRHYLRKACLRLNHPVPEIAGELLKGIMRRAWPSNVRELANAA 336
+ L R +DI L RH++++A + V E L+ + WP NVREL N
Sbjct: 304 PLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 337 ELFAV-----------------------------------GVLPLAETVNPQLL------ 355
+ E Q
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 356 LQEPTPLDRRVEEYERQIITEALNIHQGRINEVAEYLQIPRKKLYLRMKKYGLS 409
L DR + E E +I AL +G + A+ L + R L ++++ G+S
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0465OMPTIN472e-172 Omptin serine protease signature.
		>OMPTIN#Omptin serine protease signature.

Length = 317

Score = 472 bits (1217), Expect = e-172
Identities = 149/320 (46%), Positives = 211/320 (65%), Gaps = 11/320 (3%)

Query: 1 MKKHAIAVMMIAVFSESVYAESTLFIPDVSPESVTTSLSVGVLNGKSRELVYD-TDTGRK 59
M+ + +++ + S +A + +P+++ +S+G L+GK++E VY + GRK
Sbjct: 1 MRAKLLGIVLTTPIAISSFASTET--LSFTPDNINADISLGTLSGKTKERVYLAEEGGRK 58

Query: 60 LSQLDWKIKNVATLQGDLSWKPYSFMTLDARGWTSLASGSGHMVDHDWMSSEQPG-WTDR 118
+SQLDWK N A ++G ++W +++ A GWT+L S G+MVD DWM S PG WTD
Sbjct: 59 VSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDE 118

Query: 119 SIHPDTSANYANEYDLNVKGWLLQGDNYKAGVTAGYQETRFSWTARGGSYIYDNGR---- 174
S HPDT NYANE+DLN+KGWLL NY+ G+ AGYQE+R+S+TARGGSYIY +
Sbjct: 119 SRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRD 178

Query: 175 YIGNFPHGVRGIGYSQRFEMPYIGLAGDYRINDFECNVLFKYSDWVNAHDNDEHY--MRK 232
IG+FP+G R IGY QRF+MPYIGL G YR DFE FKYS WV + DNDEHY ++
Sbjct: 179 DIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKR 238

Query: 233 LTFREKTENSRYYGASIDAGYYITSNAKIFAEFAYSKYEEGKGGTQIIDKTSGNTAYFGG 292
+T+R K ++ YY +++AGYY+T NAK++ E A+++ KG T + D + NT+ +
Sbjct: 239 ITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNN-NTSDYSK 297

Query: 293 DAAGIANNNYTVTAGLQYRF 312
+ AGI N N+ TAGL+Y F
Sbjct: 298 NGAGIENYNFITTAGLKYTF 317


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0471PF06580290.033 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 28.7 bits (64), Expect = 0.033
Identities = 22/113 (19%), Positives = 46/113 (40%), Gaps = 12/113 (10%)

Query: 199 WIIATMVWMFPAAGGAKIVVIILMTWLIALGDTTHIVVGSVEILYLV-FNGTLPWSDFFW 257
I W+ G I+ ++ +I + V + I L+ F T P + F
Sbjct: 61 SFIKRQGWLKLNMG-QIILRVLPACVVIGM----VWFVANTSIWRLLAFINTKPVA-FTL 114

Query: 258 PFALPTLAGNICGGTFIFALMSHAQIRNDMSNKRKEEARLRGERLERERKKAE 310
P AL ++ N+ TF+++L+ K ++A + ++ ++A+
Sbjct: 115 PLAL-SIIFNVVVVTFMWSLLYFGWHFF----KNYKQAEIDQWKMASMAQEAQ 162


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0472VACJLIPOPROT398e-144 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 398 bits (1024), Expect = e-144
Identities = 237/251 (94%), Positives = 248/251 (98%)

Query: 1 MKLRLSALALGTTLLVGCASSGTEQQGRSDPFEGFNRTMYNFNFNVLDPYVVRPVAVAWR 60
MKLRLSALALGTTLLVGCASSGT+QQGRSDP EGFNRTMYNFNFNVLDPY+VRPVAVAWR
Sbjct: 1 MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWR 60

Query: 61 DYVPQPARNGLSNFTGNLEEPAIMVNYFLQGDPYQGMVHFTRFFLNTLLGMGGFIDVAGM 120
DYVPQPARNGLSNFTGNLEEPA+MVNYFLQGDPYQGMVHFTRFFLNT+LGMGGFIDVAGM
Sbjct: 61 DYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGM 120

Query: 121 ANPKLQRVEPHRFGSTLGHYGVGYGPYMQLPFYGSFTLREDGGDMADTLYPVLSWLTWPM 180
ANPKLQR EPHRFGSTLGHYGVGYGPY+QLPFYGSFTLR+DGGDMAD LYPVLSWLTWPM
Sbjct: 121 ANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMADALYPVLSWLTWPM 180

Query: 181 SIGKWTIEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGKLKPQENPNAQA 240
S+GKWT+EGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGG+LKPQENPNAQA
Sbjct: 181 SVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQA 240

Query: 241 IQDELKEIDSE 251
IQD+LK+IDSE
Sbjct: 241 IQDDLKDIDSE 251


66SPA0496SPA0504N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0496-2152.559541tRNA pseudouridine synthase A
SPA04970151.994030DedA protein (dsg-1 protein)
SPA0498-1111.689964acetyl-CoA carboxylase subunit beta
SPA0499-1100.392882folylpolyglutamate synthase
SPA0500012-1.140245DedD protein
SPA0501010-1.928848colicin V production protein (DedE protein)
SPA0502010-1.592502amidophosphoribosyltransferase
SPA0503013-1.745602transcriptional regulator
SPA0504012-1.896279amino acid decarboxylase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0496FbpA_PF05833290.026 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 28.7 bits (64), Expect = 0.026
Identities = 20/63 (31%), Positives = 31/63 (49%), Gaps = 6/63 (9%)

Query: 204 VRNIVGS-LLEVGAHNQPESWIAELLAARDRTLAAATAKAEGLYLVAVDYPDRFDLPKPP 262
+NI GS ++ + PES + E AA LAA +K++ V VDY + ++ KP
Sbjct: 496 TKNIPGSHVIVKNIMDIPESTLLE--AAN---LAAYYSKSQNSSNVPVDYTEVKNVKKPN 550

Query: 263 MGP 265

Sbjct: 551 GAK 553


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0500PERTACTIN290.023 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 28.5 bits (63), Expect = 0.023
Identities = 16/50 (32%), Positives = 18/50 (36%)

Query: 105 SKPKPVEKPKPQPKPQQPVVAASTPTPAPQPVADDKPAPTGKAYVVQLGA 154
+K P KP PQP PQ P P P P +A Q A
Sbjct: 565 AKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAPQPPA 614



Score = 27.8 bits (61), Expect = 0.049
Identities = 19/60 (31%), Positives = 22/60 (36%), Gaps = 4/60 (6%)

Query: 99 PIPAETSKPKPVEKPKPQPKPQQPVVAASTPTPAPQPVADDKPAPTGKAYVVQLGALKNA 158
P P +P P P+P PQ P P QP A P G+ L A NA
Sbjct: 569 PAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAPQPPAGRE----LSAAANA 624


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0502ANTHRAXTOXNA340.002 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 33.6 bits (76), Expect = 0.002
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 2/37 (5%)

Query: 469 KDVDQQYLDFLDSLRND-DAKAVLFQNEM-ENLEMHN 503
K +D ++L+ + SL +D D+ +LF + E LE++N
Sbjct: 186 KSLDPEFLNLIKSLSDDSDSSDLLFSQKFKEKLELNN 222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0503HTHFIS348e-118 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 348 bits (894), Expect = e-118
Identities = 121/371 (32%), Positives = 185/371 (49%), Gaps = 24/371 (6%)

Query: 122 NMSGVRRLQEQVVELNQLLYADHHE---KHHAIITENPEMLSNIAKAKRLAASNIPVTIV 178
+++ + + + + + + + ++ + M RL +++ + I
Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMIT 166

Query: 179 GETGTGKELFSRLIHQCSKRANKPFIALNCGALPPTLIESTLFGTVRGAYTGAENS-QGY 237
GE+GTGKEL +R +H KR N PF+A+N A+P LIES LFG +GA+TGA+ G
Sbjct: 167 GESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGR 226

Query: 238 LELANGGTLFLDELNAMPIEMQSKLLRFLQDKTFWRLGGQQQLHSDVRIVAAMNEAPVKL 297
E A GGTLFLDE+ MP++ Q++LLR LQ + +GG+ + SDVRIVAA N+ +
Sbjct: 227 FEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQS 286

Query: 298 IQQERLRADLFYRLSVGMLTLPPLRARPEDIPLLANYFIDKYRNDVPQDIHGLSETARAD 357
I Q R DL+YRL+V L LPPLR R EDIP L +F+ + + D+ + A
Sbjct: 287 INQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALEL 345

Query: 358 LLNHAWPGNVRMLENAIVRSMIMQEKDGLLKHIIF-------------------EQDELN 398
+ H WPGNVR LEN + R + +D + + II ++
Sbjct: 346 MKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSIS 405

Query: 399 LGVPETAPENPLPSSPDPQYEGSLEVRVANYERHLIETALDTHQGNIAAAARSLNVSRTT 458
V E + G + +A E LI AL +GN AA L ++R T
Sbjct: 406 QAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNT 465

Query: 459 LQYKVQKYAIR 469
L+ K+++ +
Sbjct: 466 LRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0504ALARACEMASE320.006 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 31.7 bits (72), Expect = 0.006
Identities = 23/133 (17%), Positives = 47/133 (35%), Gaps = 20/133 (15%)

Query: 87 VLKAIRDAGICAEANSQYEVRKCLEIGFRGDQIVFNGVVKKPADLEYAIANDLYLINVDS 146
+ AI A N + E E G++G ++ G DLE + L
Sbjct: 46 IWSAIGATDGFALLNLE-EAITLRERGWKGPILMLEGFFH-AQDLEIYDQHRLTT----C 99

Query: 147 LYELEHIDAIS-RKLKKVANVCVRVEPNVPSATHAELVTAFHAKSGLDLEQAEETCRRIL 205
++ + A+ +LK ++ ++V + + + G ++ +++
Sbjct: 100 VHSNWQLKALQNARLKAPLDIYLKVN------------SGMN-RLGFQPDRVLTVWQQLR 146

Query: 206 AMPYVHLRGLHMH 218
AM V L H
Sbjct: 147 AMANVGEMTLMSH 159


67SPA0589SPA0597N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0589-19-3.426570hypothetical protein
SPA0590-19-2.383113hypothetical protein
SPA0591013-1.096877MR-MLE-family protein
SPA05920140.307383DNA gyrase subunit A
SPA0593-2120.501652sensor protein RcsC
SPA0594-2131.025684regulator of capsule synthesis B component
SPA0595-3111.450725two-component system sensor kinase
SPA0597-2152.959943outer membrane protein C
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0589NUCEPIMERASE280.031 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 28.2 bits (63), Expect = 0.031
Identities = 16/75 (21%), Positives = 29/75 (38%), Gaps = 15/75 (20%)

Query: 133 AERDTQAYLKLDHDFHYVFVKYADNKYISQAHLLISARLLAIRYRLDFTAEYITSSNRGH 192
A+R+ L F VF+ + A+RY L+ Y S+ G
Sbjct: 62 ADREGMTDLFASGHFERVFISPH------RL---------AVRYSLENPHAYADSNLTGF 106

Query: 193 ATILDMLKNNNVEGV 207
IL+ ++N ++ +
Sbjct: 107 LNILEGCRHNKIQHL 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0590TCRTETB310.012 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 30.6 bits (69), Expect = 0.012
Identities = 36/179 (20%), Positives = 68/179 (37%), Gaps = 12/179 (6%)

Query: 25 ILYFFNYMDRVNIGFAALRMNESLGITPEDFANISSIFFISYLIFQIPSSIGLQKLGARK 84
IL FF+ ++ + + + + P +++ F +++ I +LG ++
Sbjct: 21 ILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKR 80

Query: 85 W--ISSIIIGWGAVTGLIFFAKDTQHIL-LARIFLGVFEAGFFPGMVYYLACWFPARERG 141
II +G+V G F +L +AR G A F ++ +A + P RG
Sbjct: 81 LLLFGIIINCFGSVIG--FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138

Query: 142 KVNSFFMLSIAVASVLAAPMSGWIIEHLNTPDYEGWRWLFAIEGIPTVFLGILTFYLLP 200
K +A+ + + G I ++ W +L I I T+ LL
Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYI------HWSYLLLIPMI-TIITVPFLMKLLK 190


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0593HTHFIS801e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 79.9 bits (197), Expect = 1e-17
Identities = 29/104 (27%), Positives = 47/104 (45%)

Query: 827 ILVVDDHPINRRLLADQLGSLGYQCKTANDGVDALNVLSKNAIDIVLSDVNMPNMDGYRL 886
ILV DD R +L L GY + ++ ++ D+V++DV MP+ + + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 887 TQRIRQLGLTLPVVGVTANALAEEKQRCLESGMDSCLSKPVTLD 930
RI++ LPV+ ++A + E G L KP L
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0594HTHFIS488e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 47.9 bits (114), Expect = 8e-09
Identities = 26/145 (17%), Positives = 60/145 (41%), Gaps = 20/145 (13%)

Query: 1 MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLPKLDAHVLITDLSMP 60
M +++ADD + + ++L + + + ++ L + D +++TD+ MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRI--TSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 GDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGA------PT 114
+ L+ IK+ P L ++V++ N +A+ ++GA P
Sbjct: 59 D---ENAFDLLPRIKKARPDLPVLVMSAQNTFM--TAIKA-------SEKGAYDYLPKPF 106

Query: 115 DLPKALAALQKGKKFTPESVSRLLE 139
DL + + + + S+L +
Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0597ECOLIPORIN5400.0 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 540 bits (1392), Expect = 0.0
Identities = 261/389 (67%), Positives = 298/389 (76%), Gaps = 17/389 (4%)

Query: 1 MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLFGKVDGLHYFSDDKGSDGDQTYMRIG 60
MK KVL+L++PALL AGAA+AAEIYNKDGNKLDL+GKVDGLHYFSDD DGDQTYMR+G
Sbjct: 1 MKRKVLALVIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDDSSKDGDQTYMRVG 60

Query: 61 FKGETQVNDQLTGYGQWEYQIQGNQTEG-SNDSWTRVAFAGLKFADAGSFDYGRNYGVTY 119
FKGETQ+NDQLTGYGQWEY +Q N TEG +SWTR+AFAGLKF D GSFDYGRNYGV Y
Sbjct: 61 FKGETQINDQLTGYGQWEYNVQANTTEGEGANSWTRLAFAGLKFGDYGSFDYGRNYGVLY 120

Query: 120 DVTSWTDVLPEFGGDTYG-ADNFMQQRGNGYATYRNTDFFGLVDGLDFALQYQGKNGSVS 178
DV WTD+LPEFGGD+Y ADN+M R NG ATYRNTDFFGLVDGL+FALQYQGKN S S
Sbjct: 121 DVEGWTDMLPEFGGDSYTYADNYMTGRANGVATYRNTDFFGLVDGLNFALQYQGKNESQS 180

Query: 179 GEN--------TNGRSLLNQNGDGYGGSLTYAIGEGFSVGGAITTSKRTADQNNTANARL 230
++ NG + NGDG+G S TY IG GFS G A TTS RT +Q N
Sbjct: 181 ADDVNIGTNNRNNGDDIRYDNGDGFGISTTYDIGMGFSAGAAYTTSDRTNEQVNAGG--T 238

Query: 231 YGNGDRATVYTGGLKYDANNIYLAAQYSQTYNATRFGTSNGSNPSTSYGFANKAQNFEVV 290
GD+A +T GLKYDANNIYLA YS+T N T +G ++ G ANK QNFEV
Sbjct: 239 IAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPYGKTDK---GYDGGVANKTQNFEVT 295

Query: 291 AQYQFDFGLRPSVAYLQSKGKDISNGYGASYGDQDIVKYVDVGATYYFNKNMSTYVDYKI 350
AQYQFDFGLRP+V++L SKGKD++ + D+D+VKY DVGATYYFNKN STYVDYKI
Sbjct: 296 AQYQFDFGLRPAVSFLMSKGKDLTYN-NVNGDDKDLVKYADVGATYYFNKNFSTYVDYKI 354

Query: 351 NLLDKND-FTRDAGINTDDIVALGLVYQF 378
NLLD +D F +DAGI+TDDIVALG+VYQF
Sbjct: 355 NLLDDDDPFYKDAGISTDDIVALGMVYQF 383


68SPA0734SPA0741N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0734-1153.752068two-component system response regulator
SPA0735-1164.259376two-component system sensor kinase
SPA0736-1163.499820transporter protein
SPA0737-1153.229573RND-family transporter protein
SPA07380153.368001RND-family transporter protein
SPA0741-1132.623066hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0734HTHFIS758e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.3 bits (185), Expect = 8e-18
Identities = 27/140 (19%), Positives = 65/140 (46%), Gaps = 2/140 (1%)

Query: 11 PRILIVEDEPKLGQLLIDYLRAASYAPTLINHGDKVLPYVRQTPPDLILLDLMLPGTDGL 70
IL+ +D+ + +L L A Y + ++ + ++ DL++ D+++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 71 TLCREIR-RFSDIPIVMVTAKIEEIDRLLGLEIGADDYICKPYSPREVVARVKTIL-RRC 128
L I+ D+P+++++A+ + + E GA DY+ KP+ E++ + L
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 129 KPQRELQQQDAESPLMIDES 148
+ +L+ + ++ S
Sbjct: 124 RRPSKLEDDSQDGMPLVGRS 143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0735BCTERIALGSPF310.010 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 31.0 bits (70), Expect = 0.010
Identities = 20/66 (30%), Positives = 26/66 (39%), Gaps = 14/66 (21%)

Query: 187 RGLLAPVKRLVEGTHRLAAGDFTTRVTPTSADEL-----------GKLAQDFNQLASTLE 235
L+A V+ V H LA + P S + L G L N+LA E
Sbjct: 104 SQLMAAVRSKVMEGHSLAD---AMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTE 160

Query: 236 KNQQMR 241
+ QQMR
Sbjct: 161 QRQQMR 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0736TCRTETB1243e-33 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 124 bits (313), Expect = 3e-33
Identities = 95/450 (21%), Positives = 197/450 (43%), Gaps = 25/450 (5%)

Query: 20 FMQSLDTTIVNTALPSMAKSLGESPLHMHMVVVSYVLTVAVMLPASGWLADKIGVRNIFF 79
F L+ ++N +LP +A + P + V +++LT ++ G L+D++G++ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 80 AAIVLFTLGSLFCALSGTLNQ-LVLARVLQGVGGAMMVPVGRLTVMKIVPRAQYMAAMTF 138
I++ GS+ + + L++AR +QG G A + + V + +P+ A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 139 VTLPGQIGPLLGPALGGVLVEYASWHWIFLINIP-VGIVGAMATFMLMPNYTIETRRFDL 197
+ +G +GPA+GG++ Y HW +L+ IP + I+ L+ FD+
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 198 PGFLLLAIGMAVLTLALDGSKSMGISPWTLAGLAAGGAAAILLYLFHAKKSSGALFSLRL 257
G +L+++G+ L + L + L+++ H +K + L
Sbjct: 202 KGIILMSVGIVFFMLFTTSYSISFLIVSVL---------SFLIFVKHIRKVTDPFVDPGL 252

Query: 258 FRTPTFSLGLLGSFAGRIGSGMLPFMTPVFLQIGLGFSPFHAG-LMMIPMVLGSMGMKRI 316
+ F +G+L M P ++ S G +++ P + + I
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312

Query: 317 VVQIVNRFGYRRVLVATTLGLALVSLLFMSVALL----GWYYLLPLVLLLQGMVNSARFS 372
+V+R G VL +G+ +S+ F++ + L W+ + +V +L G+ S +
Sbjct: 313 GGILVDRRGPLYVL---NIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL--SFTKT 367

Query: 373 SMNTLTLKDLPDTLASSGNSLLSMIMQLSMSIGVTIAGMLL--GMFGQQHIGIDSSATHH 430
++T+ L A +G SLL+ LS G+ I G LL + Q+ + ++ + +
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTY 427

Query: 431 VFMYTWLCMAVIIALPAIIFARVPNDTQQN 460
++ L + II + ++ V +Q++
Sbjct: 428 LYSNLLLLFSGIIVISWLVTLNVYKHSQRD 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0737ACRIFLAVINRP8750.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 875 bits (2263), Expect = 0.0
Identities = 282/1035 (27%), Positives = 503/1035 (48%), Gaps = 36/1035 (3%)

Query: 6 LFIYRPVATILIAAAITLCGILGFRLLPVAPLPQVDFPVIMVSASLPGASPETMASSVAT 65
FI RP+ ++A + + G L LPVA P + P + VSA+ PGA +T+ +V
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 66 PLERSLGRIAGVNEMTSSS-SLGSTRIILEFNFDRDINGAARDVQAAINAAQSLLPGGMP 124
+E+++ I + M+S+S S GS I L F D + A VQ + A LLP +
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 125 SRPTYRKANPSDAPIMILTLTSES--WSQGKLYDFASTQLAQTIAQIDGVGDVDVGGSSL 182
+ S + +M+ S++ +Q + D+ ++ + T+++++GVGDV + G+
Sbjct: 124 -QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY 182

Query: 183 PAVRVGLNPQALFNQGVSLDEVREAIDSANVRRPQGAIEDSV------HRWQIQTNDELK 236
A+R+ L+ L ++ +V + N + G + + I K
Sbjct: 183 -AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 237 TAAEYQPLIIHYN-NGAAVRLGDVASVTDSVQDVRNAGMTNAKPAILLMIRKLPEANIIQ 295
E+ + + N +G+ VRL DVA V ++ N KPA L I+ AN +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 296 TVDGIRAKLPELRAMIPAAIDLQIAQDRSPTIRASLQEVEETLAISVALVILVVFLFLRS 355
T I+AKL EL+ P + + D +P ++ S+ EV +TL ++ LV LV++LFL++
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 356 GRATLIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMALTIATGFVVDDAIVVLENIARHL- 414
RATLIP +AVPV L+GTFA + G+S+N L++ + +A G +VDDAIVV+EN+ R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 EAGMKPLQAALQGTREVGFTVISMSLSLVAVFLPLLLMGGLPGRLLREFAVTLSVAIGIS 474
E + P +A + ++ ++ +++ L AVF+P+ GG G + R+F++T+ A+ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 475 LVVSLTLTPMMCGWMLKSSKPRTQPRKRGVG----RLLVALQQGYGTSLKWVLNHTRLVG 530
++V+L LTP +C +LK K G Y S+ +L T
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 531 VVFLGTVALNIWLYIAIPKTFFPEQDTGVLMGGIQADQSISFQ----AMRGKLQDFMKII 586
+++ VA + L++ +P +F PE+D GV + IQ + + + ++K
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 587 RD-DPAVNNVTGFT-GGSRVNSGMMFITLKPRGER---KETAQQIIDRLRVKLAKEPGAR 641
+ +V V GF+ G N+GM F++LKP ER + +A+ +I R +++L K
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 642 LFLMAVQDIRVGGRQANASYQYTLLSDSLPALREWEPKIRKALSAL-----PQLADVNSD 696
+ + I G ++ L D + + R L + L V +
Sbjct: 662 VIPFNMPAIVELGTATGFDFE---LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 697 QQDNGAEMNLIYDRDTMSRLGIDVQAANSLLNNTFGQRQISTIYQPMNQYKVVMEVDPRY 756
++ A+ L D++ LG+ + N ++ G ++ K+ ++ D ++
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 757 TQDISALEKMFVINRDGKAIPLSYFAQWRPANAPLSVNHQGLSAASTIAFNLPTGTSLSQ 816
++K++V + +G+ +P S F + + I GTS
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 817 ATEAINRTMTQLGVPSTVRGSFSGTAQVFQQTMNSQLILIVAAIATVYIVLEILYESYVH 876
A + ++L P+ + ++G + + + N L+ + V++ L LYES+
Sbjct: 839 AMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSI 896

Query: 877 PLTILSTLPSASVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRSGG 936
P++++ +P VG LLA LFN + ++G++ IG+ KNAI++V+FA + G
Sbjct: 897 PVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 937 LTPEQAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLGITIVGGLVMSQLL 996
+A A +R RPI+MT+LA + G LPL +S G GS + +GI ++GG+V + LL
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 997 TLYTTPVVYLFFDRL 1011
++ PV ++ R
Sbjct: 1017 AIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0738ACRIFLAVINRP8850.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 885 bits (2289), Expect = 0.0
Identities = 292/1036 (28%), Positives = 504/1036 (48%), Gaps = 29/1036 (2%)

Query: 13 SRLFILRPVATTLLMAAILLAGIIGYRFLPVAALPEVDYPTIQVVTLYPGASPDVMTSSV 72
+ FI RP+ +L +++AG + LPVA P + P + V YPGA + +V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 73 TAPLERQFGQMSGLKQMSSQS-SGGASVVTLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131
T +E+ + L MSS S S G+ +TL FQ D+A+ +VQ + AT LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 132 LPNPPIYSKVNPADPPIMTLAVTSNAMPMTQVE--DMVETRVAQKISQVSGVGLVTLAGG 189
+ I S + +M S+ TQ + D V + V +S+++GVG V L G
Sbjct: 122 VQQQGI-SVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 190 QRPAIRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSLDGP------ERAVTLSANDQ 243
Q A+R+ L+A + LT V + N A G L G + ++ A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 244 MQSADEYRKLII-AYQNGAPVRLGDVATVEQGAENSWLGAWANQAPAIVMNVQRQPGANI 302
++ +E+ K+ + +G+ VRL DVA VE G EN + A N PA + ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 303 IATADSIRQMLPQLTESLPKSVKVTVLSDRTTNIRASVRDTQFELMLAIALVVMIIYLFL 362
+ TA +I+ L +L P+ +KV D T ++ S+ + L AI LV +++YLFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 363 RNIPATIIPGVAVPLSLIGTFAVMVFLDFSINNLTLMALTIATGFVVDDAIVVIENISRY 422
+N+ AT+IP +AVP+ L+GTFA++ +SIN LT+ + +A G +VDDAIVV+EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 423 I-EKGEKPLAAALKGAGEIGFTIISLTFSLIAVLIPLLFMGDIVGRLFREFAVTLAVAIL 481
+ E P A K +I ++ + L AV IP+ F G G ++R+F++T+ A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 482 ISAVVSLTLTPMMCACML---SQQSLRKQNRFSRACERMFDRVIASYGRGLAKVLNHPWL 538
+S +V+L LTP +CA +L S + + F FD + Y + K+L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 539 TLSVAFATLLLSVMLWIVIPKGFFPVQDNGIIQGTLQAPQSSSYASMAQRQRQVAERILQ 598
L + + V+L++ +P F P +D G+ +Q P ++ + QV + L+
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 599 DPA--VQSLTTFVGVDGANPTLNSARLQINLKPLDARDDR---VQQVISRLQTAVATIPV 653
+ V+S+ T G + N+ ++LKP + R+ + VI R + + I
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 654 VALYLQPTQDLTIDTQVSRTQYQFTLQ---ATTLDALSHWVPKL-QNALQSLPQLSEVSS 709
++ P I + T + F L DAL+ +L A Q L V
Sbjct: 660 G--FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRP 717

Query: 710 DWQDRGLAAWVNVDRDSASRLGISIADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTA 769
+ + + VD++ A LG+S++D++ + A G ++ + ++ ++ +
Sbjct: 718 NGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAK 777

Query: 770 STPGLAALETIRLTSRDGGTVSLSAIARIEQRFAPLSINHLDQFPVTTFSFNVPEGYSLG 829
++ + + S +G V SA + + + P G S G
Sbjct: 778 FRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG 837

Query: 830 DAVQAILDTEKTLALPADITTQFQGSTLAFQAALGSTVWLIVAAVVAMYIVLGVLYESFI 889
DA+ + + LPA I + G + + + L+ + V +++ L LYES+
Sbjct: 838 DAMALMENLAS--KLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWS 895

Query: 890 HPITILSTLPTAGVGALLALIIAGSELDIIAIIGIILLIGIVKKNAIMMIDFALAAEREQ 949
P++++ +P VG LLA + + D+ ++G++ IG+ KNAI++++FA ++
Sbjct: 896 IPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKE 955

Query: 950 GMSPRDAIFQACLLRFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIAMVGGLLVSQV 1009
G +A A +R RPILMT+LA +LG LPL +S G G+ + +GI ++GG++ + +
Sbjct: 956 GKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATL 1015

Query: 1010 LTLFTTPVIYLLFDRL 1025
L +F PV +++ R
Sbjct: 1016 LAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0741SHAPEPROTEIN515e-09 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 50.5 bits (121), Expect = 5e-09
Identities = 32/129 (24%), Positives = 56/129 (43%), Gaps = 20/129 (15%)

Query: 132 AMMVHIRHTAHSQ-LPEAITQAVIGRPINFQGLGGDDANRQAQGILERAAKRAGFQEVVF 190
M+ H HS + ++ P+ R+A + +A+ AG +EV
Sbjct: 89 KMLQHFIKQVHSNSFMRPSPRVLVCVPVGA-----TQVERRA---IRESAQGAGAREVFL 140

Query: 191 QYEPVAAGLDYEATLREEKRVLVVDIGGGTTDCSMLLMGPQWRQRADRENSLLGHSGCRV 250
EP+AA + + E +VVDIGGGTT+ +++ + ++ S R+
Sbjct: 141 IEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN-----------GVVYSSSVRI 189

Query: 251 GGNDLDIAL 259
GG+ D A+
Sbjct: 190 GGDRFDEAI 198



Score = 34.3 bits (79), Expect = 7e-04
Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 12/81 (14%)

Query: 377 ALDQPLARILEQVQLALDSAQEKPDV--------IYLTGGSARSPLIKKALSEQLPGIPV 428
AL +PL I+ V +AL+ Q P++ + LTGG A + + L E+ GIPV
Sbjct: 259 ALQEPLTGIVSAVMVALE--QCPPELASDISERGMVLTGGGALLRNLDRLLMEET-GIPV 315

Query: 429 AGGDD-FGSVTAGLARWAEVV 448
+D V G + E++
Sbjct: 316 VVAEDPLTCVARGGGKALEMI 336


69SPA0769SPA0778N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0769437-9.111934dTDP-glucose 4,6-dehydratase
SPA0770540-9.196286dTDP-4-dehydrorhamnose reductase
SPA0771842-10.181874TDP-glucose pyrophosphorylase
SPA0772946-11.903907dTDP-4-dehydrorhamnose 3,5-epimerase
SPA0773949-12.863714reductase RfbI
SPA0774752-14.712838glucose-1-phosphate cytidylyltransferase
SPA0775755-16.252676CDP-glucose 4,6-dehydratase
SPA0776859-18.248413dehydratase RfbH
SPA0777864-20.507307paratose synthase
SPA0778865-20.719682CDP-tyvelose-2-epimerase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0769NUCEPIMERASE1761e-54 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 176 bits (449), Expect = 1e-54
Identities = 83/359 (23%), Positives = 142/359 (39%), Gaps = 50/359 (13%)

Query: 1 MKILITGGAGFIGSAVVRHIIKNTQDTVVNIDKLT--YAGNLE--SLSDISESNRYNFEH 56
MK L+TG AGFIG V + +++ VV ID L Y +L+ L +++ + F
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAG-HQVVGIDNLNDYYDVSLKQARLELLAQPG-FQFHK 58

Query: 57 ADICDSAEITRIFEQYQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEVARKYWS 116
D+ D +T +F + V V S+ P A+ ++N+ G +LE R
Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN-- 116

Query: 117 ALGEDKKNNFRFHHISTDEVYGDLPHPDEVENSVTLPLFTETTAYAPSSPYSASKASSDH 176
+ S+ VYG +P T+ + P S Y+A+K +++
Sbjct: 117 -------KIQHLLYASSSSVYGLNRK---------MPFSTDDSVDHPVSLYAATKKANEL 160

Query: 177 LVRAWRRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWLYV 236
+ + YGLP YGP+ P+ + LEGK + +Y G RD+ Y+
Sbjct: 161 MAHTYSHLYGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYI 220

Query: 237 EDHA----RALHMVVTEGKA--------------GETYNIGGHNEKKNLDVVFTICDLLD 278
+D A R ++ YNIG + + +D + + D L
Sbjct: 221 DDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALG 280

Query: 279 EIVPKATSYREQITYVADRPGHDRRYAIDAGKISRELGWKPLETFESGIRKTVEWYLAN 337
+A + + +PG + D + +G+ P T + G++ V WY
Sbjct: 281 ---IEA-----KKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0770NUCEPIMERASE413e-06 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 40.9 bits (96), Expect = 3e-06
Identities = 27/160 (16%), Positives = 57/160 (35%), Gaps = 23/160 (14%)

Query: 1 MNILLFGKTGQVGWELQRSLAPVGN-LIALDV-----------HSKEFC---------GD 39
M L+ G G +G+ + + L G+ ++ +D E D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 40 FSNPKGVAETVRKLRPDVIVNAAAHTAVDKAESEPELAQLLNATSVEAIAKAANEIG-AW 98
++ +G+ + + + + AV + P N T I +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 99 VVHYSTDYVFPGTGDIPWQETDATS-PLNVYGKTKLAGEK 137
+++ S+ V+ +P+ D+ P+++Y TK A E
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0775NUCEPIMERASE731e-16 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 73.3 bits (180), Expect = 1e-16
Identities = 62/352 (17%), Positives = 121/352 (34%), Gaps = 48/352 (13%)

Query: 11 RVFVTGHTGFKGSWLSLWLTEMGAIVKGYALDAPTVPSLFEIVRLNDLMES----HIGDI 66
+ VTG GF G +S L E G V G + RL L + H D+
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 67 RDFEKLRSSIAEFKPEIVFHMAAQPLVRLSYEQPIETYSTNVMGTVHLLEAVKQVDNIKA 126
D E + A E VF + VR S E P +N+ G +++LE + I+
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN-KIQH 120

Query: 127 VVNITSDKCYDNREWVWGYRENEPMGGYD-------PYSNSKGCAELVASAFRNSFFNPA 179
++ +S V+G P D Y+ +K EL+A + + +
Sbjct: 121 LLYASSSS-------VYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLY---- 169

Query: 180 NYEQHGVGLASVRAGNVIGGGDWAK-DRLIPDILRSFENNQQVIIRNPYSI-RPWQHVLE 237
G+ +R V G W + D + ++ + + + N + R + ++ +
Sbjct: 170 -----GLPATGLRFFTVY--GPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDD 222

Query: 238 PLSGYIVVAQRLYTEGAKFSEG-------------WNFGPRDEDAKTVEFIVDKMVTLWG 284
I + + +++ +N G + + + + G
Sbjct: 223 IAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIG--NSSPVELMDYIQALEDALG 280

Query: 285 DDASWLLDGENHPHEAHYLKLDCSKANMQLGWHPRWGLTETLSRIVKWHKAW 336
+A + P + D +G+ P + + + V W++ +
Sbjct: 281 IEAKKNML-PLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0776PERTACTIN310.012 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 30.8 bits (69), Expect = 0.012
Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 9/82 (10%)

Query: 209 GDIGTVSFYPAHHITMGEGGAVFTKSGELKKIIESFRDWGRDCYCAPGCDNTCGKRFGQQ 268
G +G S + E A+ + GEL+ ++ WGR DN G+RF Q+
Sbjct: 629 GGVGLAS-----TLWYAESNALSKRLGELRLNPDAGGAWGRGFAQRQQLDNRAGRRFDQK 683

Query: 269 LGSLPQGYDHKYTYS----HLG 286
+ G DH + HLG
Sbjct: 684 VAGFELGADHAVAVAGGRWHLG 705


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0777NUCEPIMERASE646e-14 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 64.0 bits (156), Expect = 6e-14
Identities = 58/329 (17%), Positives = 115/329 (34%), Gaps = 57/329 (17%)

Query: 1 MKILIMGAFGFLGSRLTSYFESR-HTVIGL---------ARKRNNEATINNIIYT----- 45
MK L+ GA GF+G ++ H V+G+ + K+ + +
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 46 -TENNWIEKIL-EFEPNIIINTIACYG-RHN-EPATALIESNILMPIRVLE--------- 92
+ + + + + R++ E A +SN+ + +LE
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 93 ----SISSL--DAVFINCGTSLPPNT--SLYAYTKQKANEFAAAIIDKVCG-KYIELKLE 143
S SS+ + T + SLYA TK KANE A + G L+
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATK-KANELMAHTYSHLYGLPATGLRFF 179

Query: 144 HFYGAFDGDDKFTSMVIRRCLSNQPVKL-TSGLQQRDFLYIKDL----LTAFDCIISNVN 198
YG + D + L + + + G +RDF YI D+ + D I
Sbjct: 180 TVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADT 239

Query: 199 NFPKFHS-----------IEVGSGEATSIREYVETVKNITKSNSIIEFGVVKERVNELMY 247
+ +G+ + +Y++ +++ + + + +++
Sbjct: 240 QWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNM--LPLQPGDVLE 297

Query: 248 SCADIAELEK-IGWKREFSLVDALTEIIE 275
+ AD L + IG+ E ++ D + +
Sbjct: 298 TSADTKALYEVIGFTPETTVKDGVKNFVN 326


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0778NUCEPIMERASE1572e-47 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 157 bits (398), Expect = 2e-47
Identities = 84/355 (23%), Positives = 151/355 (42%), Gaps = 55/355 (15%)

Query: 9 LITGGCGFLGSNLASFALSQGIDLIVFDNL------SRKGATDNLHWLSSLGNFEFVHGD 62
L+TG GF+G +++ L G ++ DNL S K A L L+ F+F D
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQA--RLELLAQ-PGFQFHKID 60

Query: 63 IRNKNDVTRLITKYMPDSCFHLAGQVAMTTSIDNPCMDFEINVGGTLNLLEAVRQYNSNC 122
+ ++ +T L + F ++A+ S++NP + N+ G LN+LE R
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ- 119

Query: 123 NIIYSSTNKVYGDLEQYKYNETETRYTCVDKPNGYDESTQLDFHSPYGCSKGAADQYMLD 182
+++Y+S++ VYG + ++ ++ VD P S Y +K A +
Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDS----VDHP-----------VSLYAATKKANELMAHT 164

Query: 183 YARIFGLNTVVFRHSSMYG--GRQFATYDQGWVGWFCQKAVEIKNGINKPFTISGNGKQV 240
Y+ ++GL R ++YG GR + F + + G K + GK
Sbjct: 165 YSHLYGLPATGLRFFTVYGPWGRPDMALFK-----FTKA---MLEG--KSIDVYNYGKMK 214

Query: 241 RDVLHAEDMI-------SLYFTALANVSKIRGNA---------FNIGGTIVNSLSLLELF 284
RD + +D+ + A + G +NIG + + + L++
Sbjct: 215 RDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNS--SPVELMDYI 272

Query: 285 KLLEDYCNIDMRFTNLPVRESDQRVFVADIKKITNAIDWSPKVSAKDGVQKMYDW 339
+ LED I+ + LP++ D AD K + I ++P+ + KDGV+ +W
Sbjct: 273 QALEDALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNW 327


70SPA0812SPA0819N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0812-2170.951721hypothetical protein
SPA0813-1163.483143hypothetical protein
SPA0814-1204.512334propionate kinase
SPA08150255.770481propanediol utilization protein PduV
SPA08161266.258271propanediol utilization protein PduU
SPA08171266.676313propanediol utilization protein PduT
SPA08182246.958389ferredoxin
SPA08193246.446243propanol dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0812FbpA_PF05833270.023 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 26.8 bits (59), Expect = 0.023
Identities = 8/49 (16%), Positives = 25/49 (51%)

Query: 16 RLFRRKNKLQREIQDIEKKIRDNQKRVLLLDNLSDYIKPGMSVEAIQGI 64
+++ NKL++ + +++ N++ + L ++ I + + I+ I
Sbjct: 385 SYYKKYNKLKKSEEAANEQLLQNEEELNYLYSVLTNINNADNYDEIEEI 433


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0814ACETATEKNASE5820.0 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 582 bits (1501), Expect = 0.0
Identities = 200/395 (50%), Positives = 279/395 (70%), Gaps = 5/395 (1%)

Query: 4 KIMAINAGSSSLKFQLLEMPQGDMLCQGLIERIGMADAQVTIKTHNQKWQETVPVADHRD 63
KI+ IN GSSSLK+QL+E G++L +GL ERIG+ D+ +T + +K + + DH+D
Sbjct: 2 KILVINCGSSSLKYQLIESKDGNVLAKGLAERIGINDSLLTHNANGEKIKIKKDMKDHKD 61

Query: 64 AVTLLLEKLLG--YQIINSLRDIDGVGHRVAHGGEFFKDSTLVTDETLAQIERLAELAPL 121
A+ L+L+ L+ Y +I + +ID VGHRV HGGE+F S L+TD+ L I ELAPL
Sbjct: 62 AIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGEYFTSSVLITDDVLKAITDCIELAPL 121

Query: 122 HNPVNALGIHVFRQLLPDAPSVAVFDTAFHQTLDEPAYIYPLPWHYYAELGIRRYGFHGT 181
HNP N GI Q++PD P VAVFDTAFHQT+ + AY+YP+P+ YY + IR+YGFHGT
Sbjct: 122 HNPANIEGIKACTQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRKYGFHGT 181

Query: 182 SHKYVSGVLAEKLGVPLSALRVICCHLGNGSSVCAIKNGRSVNTSMGFTPQSGVMMGTRS 241
SHKYVS AE L P+ +L++I CHLGNGSS+ A+KNG+S++TSMGFTP G+ MGTRS
Sbjct: 182 SHKYVSQRAAEILNKPIESLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGLAMGTRS 241

Query: 242 GDIDPSILPWIAQRESKTPQQLNQLLNNESGLLGVSGVSSDYRDVEQAA-NTGNRQAKLA 300
G IDPSI+ ++ ++E+ + +++ +LN +SG+ G+SG+SSD+RD+E AA G+++A+LA
Sbjct: 242 GSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRAQLA 301

Query: 301 LTLFAERIRATIGSYIMQMGGLDALVFTGGIGENSARARSAVCHNLQFLGLAVDEEKNQR 360
L +FA R++ TIGSY MGG+D +VFT GIGEN R + L+FLG +D+EKN+
Sbjct: 302 LNVFAYRVKKTIGSYAAAMGGVDVIVFTAGIGENGPEIREFILDGLEFLGFKLDKEKNKV 361

Query: 361 NA--TFIQTENALVKVAVINTNEELMIAQDVMRIA 393
I T ++ V V V+ TNEE MIA+D +I
Sbjct: 362 RGEEAIISTADSKVNVMVVPTNEEYMIAKDTEKIV 396


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0815SALSPVBPROT270.047 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 26.6 bits (58), Expect = 0.047
Identities = 11/24 (45%), Positives = 14/24 (58%)

Query: 93 IGLVTKADLADPQRISLVAQWLTQ 116
+G A L+DPQ S AQWL +
Sbjct: 171 LGKTAAARLSDPQAASHTAQWLVE 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0819BONTOXILYSIN300.014 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 30.3 bits (68), Expect = 0.014
Identities = 8/39 (20%), Positives = 16/39 (41%)

Query: 190 SDFIDALAEKAAKLVFQYLPTAVEKGDCVATRGKMHNAS 228
SDF ++ K LV+ +L + + + G +
Sbjct: 518 SDFSKVVSSKDKSLVYSFLDNLMSYLETIKNDGPIDTDK 556


71SPA0889SPA0905N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA08890130.443089flagellar biosynthetic protein FliR
SPA0890-1141.174655flagellar biosynthetic protein FliQ
SPA0891-1153.273445flagellar biosynthetic protein FliP
SPA08920143.210124flagellar protein FliO
SPA0893-2143.844192flagellar motor switch protein FliN
SPA0894-1174.568567flagellar motor switch protein FliM
SPA08951154.860523FliL protein
SPA08960134.834891flagellar hook-length control protein
SPA0897-1134.093197flagellar FliJ protein
SPA0898-2133.627967flagellum-specific ATP synthase
SPA0899-1132.231857flagellar assembly protein FliH
SPA0900-2141.836215flagellar motor switch protein FliG
SPA0901-2122.044523flagellar basal-body M-ring protein
SPA0902-114-0.132684flagellar hook-basal body complex protein FliE
SPA0903-215-0.686068hypothetical protein
SPA0904-414-0.096377hypothetical protein
SPA0905-311-0.887776hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0889TYPE3IMRPROT2135e-71 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 213 bits (543), Expect = 5e-71
Identities = 231/260 (88%), Positives = 246/260 (94%)

Query: 1 MIQVTSEQWLYWLHLYFWPLLRVLALISTAPILSERAIPKRVKLGLGIMITLVIAPSLPA 60
M+QVTSEQWL WL+LYFWPLLRVLALISTAPILSER++PKRVKLGL +MIT IAPSLPA
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 NDTPLFSIAALWLAMQQILIGIALGFTMQFAFAAVRTAGEFIGLQMGLSFATFVDPGSHL 120
ND P+FS ALWLA+QQILIGIALGFTMQFAFAAVRTAGE IGLQMGLSFATFVDP SHL
Sbjct: 61 NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHL 120

Query: 121 NMPVLARIMDMLAMLLFLTFNGHLWLISLLVDTFHTLPIGSNPVNSNAFMALARAGGLIF 180
NMPVLARIMDMLA+LLFLTFNGHLWLISLLVDTFHTLPIG P+NSNAF+AL +AG LIF
Sbjct: 121 NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIF 180

Query: 181 LNGLMLALPVITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGIMLMAALMPLIAPFC 240
LNGLMLALP+ITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGI LMAALMPLIAPFC
Sbjct: 181 LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC 240

Query: 241 EHLFSEIFNLLADIVSEMPI 260
EHLFSEIFNLLADI+SE+P+
Sbjct: 241 EHLFSEIFNLLADIISELPL 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0890TYPE3IMQPROT671e-18 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 67.5 bits (165), Expect = 1e-18
Identities = 23/78 (29%), Positives = 42/78 (53%)

Query: 4 ESVMMMGTEAMKVALALAAPLLLVALITGLIISILQAATQINEMTLSFIPKIVAVFIAII 63
+ ++ G +A+ + L L+ +VA I GL++ + Q TQ+ E TL F K++ V + +
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 VAGPWMLNLLLDYVRTLF 81
+ W +LL Y R +
Sbjct: 62 LLSGWYGEVLLSYGRQVI 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0891FLGBIOSNFLIP328e-117 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 328 bits (842), Expect = e-117
Identities = 224/245 (91%), Positives = 232/245 (94%)

Query: 1 MRRLLFLSLAGLWLFSPAAAAQLPGLISQPLAGGGQSWSLSVQTLVFITSLTFLPAILLM 60
MRRLL ++ LWL +P A AQLPG+ SQPL GGGQSWSL VQTLVFITSLTF+PAILLM
Sbjct: 1 MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLM 60

Query: 61 MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEQK 120
MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSE+K
Sbjct: 61 MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEK 120

Query: 121 ISMQEALDKGAQPLCAFMLRQTREADLALFARLANSGPLQGPEAVPMRILLPAYVTSELK 180
ISMQEAL+KGAQPL FMLRQTREADL LFARLAN+GPLQGPEAVPMRILLPAYVTSELK
Sbjct: 121 ISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELK 180

Query: 181 TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA 240
TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA
Sbjct: 181 TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA 240

Query: 241 QSFYS 245
QSFYS
Sbjct: 241 QSFYS 245


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0893FLGMOTORFLIN2092e-73 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 209 bits (534), Expect = 2e-73
Identities = 136/137 (99%), Positives = 136/137 (99%)

Query: 1 MSDMNNPSDENTGALDDLWADALNEQKATTNKSAADAVFQQLGGGDVSGAMQDIDLIMDI 60
MSDMNNPSDENTGALDDLWADALNEQKATT KSAADAVFQQLGGGDVSGAMQDIDLIMDI
Sbjct: 1 MSDMNNPSDENTGALDDLWADALNEQKATTTKSAADAVFQQLGGGDVSGAMQDIDLIMDI 60

Query: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120
PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV
Sbjct: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120

Query: 121 RITDIITPSERMRRLSR 137
RITDIITPSERMRRLSR
Sbjct: 121 RITDIITPSERMRRLSR 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0894FLGMOTORFLIM383e-135 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 383 bits (984), Expect = e-135
Identities = 86/324 (26%), Positives = 148/324 (45%), Gaps = 10/324 (3%)

Query: 5 ILSQAEIDALLNGDS--DTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINERFA 62
+LSQ EID LL S D E I+ I YD + +E+++ L +++E FA
Sbjct: 4 VLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFA 63

Query: 63 RQFRMGLFNLLRRSPDITVGTIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSL 122
R L LR + V ++ Y EF R++P P+ L +I + PL+G ++ PS+
Sbjct: 64 RLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPSI 123

Query: 123 VFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYVRS 182
F +D LFGG G+ KV+ R+ T E V+ ++ L ++W + L +
Sbjct: 124 TFSIIDRLFGGTGQ-AAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQI 181

Query: 183 EMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENS--R 240
E +F I P+++VV ++G G N C+P+ IEP+ L + +S R
Sbjct: 182 ETNPQFAQI-VPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRR 240

Query: 241 HEDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKP---DRIIAHVD 297
+ L ++ ++++VA + L + IL L+ GD++ + D + +
Sbjct: 241 SSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIG 300

Query: 298 GVPVLTSQYGTVNGQYALRVEHLI 321
Q G V + A ++ I
Sbjct: 301 NRKKFLCQPGVVGKKIAAQILERI 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0896FLGHOOKFLIK406e-143 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 406 bits (1045), Expect = e-143
Identities = 193/411 (46%), Positives = 233/411 (56%), Gaps = 40/411 (9%)

Query: 1 MITLPQLITTDTDMTAGLTSGKTTGSAEDFLALLAGALGADGAQGKDARITLADLQAAGG 60
MI L LIT D D T L GK + +A+DFLALL+ AL + K A L
Sbjct: 1 MIRLAPLITADVDTTT-LPGGKASDAAQDFLALLSEALAGETTTDKAAPQLL-------- 51

Query: 61 KLSKELLTQHGEPGQAVKLADLLAQKAN---ATDETLTDLTQAQHLLSTLTLSLKTSALA 117
++ + T GEP + ++D AQ+AN DET + Q + LT + + A
Sbjct: 52 -VATDKPTTKGEPLISDIVSD--AQQANLLIPVDETPPVINDEQSTSTPLTTAQTMALAA 108

Query: 118 ALSKTAQHDEKTPALSDEDLASLSALFAMLPGQPVATPVAGETPAENHIALPSLLRGDMP 177
K DEK L+++ ASLSALFAMLPG V D P
Sbjct: 109 VADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVT-----------------DAP 151

Query: 178 SAPQEETHTLSFSEHEKGKTEASLARASDDRATGPALTPLVVAAAATSAKVEVDSPPAPV 237
S F++ T L A D A G PL A +K EV S P+PV
Sbjct: 152 STVLPTEKPTLFTK----LTSEQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPV 207

Query: 238 THGAAMPTLSSATAQPQPLPVASAPVLSAPLGSHEWQQTFSQQVMLFTRQGQQSAQLRLH 297
T AA P ++ QP LP +APVLSAPLGSHEWQQ+ SQ + LFTRQGQQSA+LRLH
Sbjct: 208 T-AAASPLITPHQTQP--LPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLH 264

Query: 298 PEELGQVHISLKLDDNQAQLQMVSPHSHVRAALEAALPMLRTQLAESGIQLGQSSISSES 357
P++LG+V ISLK+DDNQAQ+QMVSPH HVRAALEAALP+LRTQLAESGIQLGQS+IS ES
Sbjct: 265 PQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGES 324

Query: 358 FAGQQQ-SSSQQQSSRAQHTDAFGAEDDIALAAPASLQAAARGNGAVDIFA 407
F+GQQQ +S QQQS R + + EDD L P SLQ GN VDIFA
Sbjct: 325 FSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0897FLGFLIJ2064e-72 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 206 bits (526), Expect = 4e-72
Identities = 130/147 (88%), Positives = 138/147 (93%)

Query: 1 MAQHGALETLKDLAEKEVDDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRSNLNTDMGNG 60
MA+HGAL TLKDLAEKEV+DAARLLGEMRRGCQQAEEQLKMLIDYQNEYR+NLN+DM G
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 IASNRWINYQQFIQTLEKAIEQHRLQLTQWTQKVDLALKSWREKKQRLQAWQTLQDRQTA 120
I SNRWINYQQFIQTLEKAI QHR QL QWTQKVD+AL SWREKKQRLQAWQTLQ+RQ+
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 AALLAENRMDQKKMDEFAQRAAMRKPE 147
AALLAENR+DQKKMDEFAQRAAMRKPE
Sbjct: 121 AALLAENRLDQKKMDEFAQRAAMRKPE 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0899FLGFLIH366e-132 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 366 bits (940), Expect = e-132
Identities = 192/235 (81%), Positives = 209/235 (88%), Gaps = 7/235 (2%)

Query: 1 MSNELPWQVWTPDDLAPPPETFVPVEAGNVTLTDDTPEPELTAEQQLEQELAQLKIQAHE 60
MS+ LPW+ WTPDDLAPP FVP+ T+ ++ AE LEQ+LAQL++QAHE
Sbjct: 1 MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEE-------AEPSLEQQLAQLQMQAHE 53

Query: 61 QGYNAGLAEGRQKGHAQGYQEGLAQGLEQGQAQAQTQQAPIHARMQQLVSEFQNTLDALD 120
QGY AG+AEGRQ+GH QGYQEGLAQGLEQG A+A++QQAPIHARMQQLVSEFQ TLDALD
Sbjct: 54 QGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALD 113

Query: 121 SVIASRLMQMALEAARQVIGQTPAVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRV 180
SVIASRLMQMALEAARQVIGQTP VDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRV
Sbjct: 114 SVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRV 173

Query: 181 EEMLGATLSLHGWRLRGDPTLHHGGCKVSADEGDLDASVATRWQELCRLAAPGVL 235
++MLGATLSLHGWRLRGDPTLH GGCKVSADEGDLDASVATRWQELCRLAAPGV+
Sbjct: 174 DDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELCRLAAPGVV 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0900FLGMOTORFLIG339e-118 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 339 bits (870), Expect = e-118
Identities = 114/329 (34%), Positives = 196/329 (59%), Gaps = 2/329 (0%)

Query: 1 MSNLSGTDKSVILLMTIGEDRAAEVFKHLSTREVQALSTAMANVRQISNKQLTDVLSEFE 60
+S L+G K+ ILL++IG + +++VFK+LS E+++L+ +A + I+++ +VL EF+
Sbjct: 12 VSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEFK 71

Query: 61 QEAEQFAALNINANEYLRSVLVKALGEERASSLLEDILETRDTTSGIETLNFMEPQSAAD 120
+ + +Y R +L K+LG ++A ++ + L + + E + +P + +
Sbjct: 72 ELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINN-LGSALQSRPFEFVRRADPANILN 130

Query: 121 LIRDEHPQIIATILVHLKRSQAADILALFDERLRHDVMLRIATFGGVQPAALAELTEVLN 180
I+ EHPQ IA IL +L +A+ IL+ ++ +V RIA P + E+ VL
Sbjct: 131 FIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVERVLE 190

Query: 181 GLLDGQ-NLKRSKMGGVRTAAEIINLMKTQQEEAVITAVREFDGELAQKIIDEMFLFENL 239
L + + GGV EIIN+ + E+ +I ++ E D ELA++I +MF+FE++
Sbjct: 191 KKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMFVFEDI 250

Query: 240 VDVDDRSIQRLLQEVDSESLLIALKGAEPPLREKFLRNMSQRAADILRDDLANRGPVRLS 299
V +DDRSIQR+L+E+D + L ALK + P++EK +NMS+RAA +L++D+ GP R
Sbjct: 251 VLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLGPTRRK 310

Query: 300 QVENEQKAILLIVRRLAETGEMVIGSGED 328
VE Q+ I+ ++R+L E GE+VI G +
Sbjct: 311 DVEESQQKIVSLIRKLEEQGEIVISRGGE 339


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0901FLGMRINGFLIF7830.0 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 783 bits (2022), Expect = 0.0
Identities = 554/559 (99%), Positives = 559/559 (100%)

Query: 5 ASTASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQ 64
++TASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQ
Sbjct: 1 SATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQ 60

Query: 65 DGGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKF 124
DGGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKF
Sbjct: 61 DGGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKF 120

Query: 125 GISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLE 184
GISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLE
Sbjct: 121 GISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLE 180

Query: 185 PGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDV 244
PGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDV
Sbjct: 181 PGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDV 240

Query: 245 ESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNIS 304
ESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNIS
Sbjct: 241 ESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNIS 300

Query: 305 EQVGAGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNNAGPRNTQRN 364
EQVGAGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSN+AGPR+TQRN
Sbjct: 301 EQVGAGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRN 360

Query: 365 ETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAMG 424
ETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAMG
Sbjct: 361 ETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAMG 420

Query: 425 FSDKRGDTLNVVNSPFSAVDDTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVR 484
FSDKRGDTLNVVNSPFSAVD+TGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVR
Sbjct: 421 FSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVR 480

Query: 485 PQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSD 544
PQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSD
Sbjct: 481 PQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSD 540

Query: 545 NDPRVVALVIRQWMSNDHE 563
NDPRVVALVIRQWMSNDHE
Sbjct: 541 NDPRVVALVIRQWMSNDHE 559


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0902FLGHOOKFLIE1141e-36 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 114 bits (286), Expect = 1e-36
Identities = 90/103 (87%), Positives = 96/103 (93%)

Query: 2 AAIQGIEGVISQLQATAMAARGQDTHSQSTVSFAGQLHAALDRISDRQTAARVQAEKFTL 61
+AIQGIEGVISQLQATAM+AR Q++ Q T+SFAGQLHAALDRISD QTAAR QAEKFTL
Sbjct: 1 SAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTL 60

Query: 62 GEPGIALNDVMADMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 104
GEPG+ALNDVM DMQKASVSMQMGIQVRNKLVAAYQEVMSMQV
Sbjct: 61 GEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0904PF01206936e-29 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 92.5 bits (230), Expect = 6e-29
Identities = 16/71 (22%), Positives = 37/71 (52%)

Query: 7 DYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCPQSINNIPLDARNHGYTVLDIQQ 66
D LD G CP P + + + + GE+L V++ P S+ + ++ G+ +L+ ++
Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64

Query: 67 DGPTIRYLIQK 77
+ T + +++
Sbjct: 65 EDGTYHFRLKR 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0905RTXTOXIND290.027 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.4 bits (66), Expect = 0.027
Identities = 10/53 (18%), Positives = 17/53 (32%), Gaps = 2/53 (3%)

Query: 184 RFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRF--RLGMLVFIGMIGWALLT 234
R L R + + + A L + P R R M + ++L
Sbjct: 26 RKQLDTPVREKDENEFLPAHLELIETPVSRRPRLVAYFIMGFLVIAFILSVLG 78


72SPA0945SPA0954N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA0945-1110.690291motility protein A
SPA0946-1120.971387motility protein B
SPA0947-1131.279346chemotaxis protein CheA
SPA0948-2141.325033purine binding chemotaxis protein
SPA0950-1121.999008chemotaxis protein methyltransferase
SPA0951-1132.689381protein-glutamate methylesterase
SPA0952-1131.776259chemotaxis protein CheY
SPA0953-2131.471293chemotaxis protein CheZ
SPA0954-2110.599744flagellar biosynthetic protein FlhB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0945PF05844320.002 YopD protein
		>PF05844#YopD protein

Length = 295

Score = 31.9 bits (72), Expect = 0.002
Identities = 12/28 (42%), Positives = 21/28 (75%), Gaps = 2/28 (7%)

Query: 76 MDLLALLYRLMAKSRQQGMFSLERDIEN 103
++LL +L+R+ K+R+ G+ L+RD EN
Sbjct: 74 VELLLILFRIAQKARELGV--LQRDNEN 99


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0946OMPADOMAIN421e-06 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 42.2 bits (99), Expect = 1e-06
Identities = 25/118 (21%), Positives = 46/118 (38%), Gaps = 11/118 (9%)

Query: 162 FKTGSAEVEPYMRDILRAIAPVL---NGIPNRISLAGHTDDFPYANGEKGYSNWELSADR 218
F A ++P + L + L + + + G+TD G Y N LS R
Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRI----GSDAY-NQGLSERR 277

Query: 219 ANASRRELVAGGLDNGKVLRVVGMAATMRLSDRGPDDAINRR--ISLLVLNKQAEQAI 274
A + L++ G+ K+ GM + ++ D+ R I L +++ E +
Sbjct: 278 AQSVVDYLISKGIPADKI-SARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0947PF06580427e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 41.8 bits (98), Expect = 7e-06
Identities = 23/151 (15%), Positives = 49/151 (32%), Gaps = 52/151 (34%)

Query: 378 ELDKSLIERIIDPLT--HLVRNSLDHGIEMPEKRLEAGKNVVGNLILSAEHQGGNICIEV 435
+++ ++++ + P+ LV N + HGI G ++L G + +EV
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIA--------QLPQGGKILLKGTKDNGTVTLEV 296

Query: 436 TDDGAGLNRERILAKAMSQGMAVNENMTDDEVGMLIFAPGFSTAEQVTDVSGRGVGMDVV 495
+ G+ + G G+ V
Sbjct: 297 ENTGSLALKNTK--------------------------------------ESTGTGLQNV 318

Query: 496 KRNIQEMGG---HVEIQSKQGSGTTIRILLP 523
+ +Q + G +++ KQG +L+P
Sbjct: 319 RERLQMLYGTEAQIKLSEKQG-KVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0951HTHFIS667e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 65.6 bits (160), Expect = 7e-14
Identities = 31/142 (21%), Positives = 62/142 (43%), Gaps = 6/142 (4%)

Query: 1 MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMP 60
M+ +L DD A +R ++ + ++ V + I + D++ DV MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 RMDGLDFLEKLMRLRPMPVVMVSSLTGKGS-EVTLRALELGAIDFVTKPQLGIREGMLAY 119
+ D L ++ + RP V+V ++ + + ++A E GA D++ KP + E +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLV--MSAQNTFMTAIKASEKGAYDYLPKP-FDLTELIGII 115

Query: 120 SEMIAEKVRTAARARIAAHKPM 141
+AE R ++ + M
Sbjct: 116 GRALAEPKRRPSKLEDDSQDGM 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0952HTHFIS897e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.7 bits (220), Expect = 7e-24
Identities = 29/105 (27%), Positives = 51/105 (48%), Gaps = 3/105 (2%)

Query: 7 KFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGFGFIISDWNMPNMDGL 66
LV DD + +R ++ L G++ V + + AG +++D MP+ +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYD-VRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 67 ELLKTIRADSAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPF 111
+LL I+ LPVL+++A+ I A++ GA Y+ KPF
Sbjct: 64 DLLPRIKKARPD--LPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA0954TYPE3IMSPROT419e-149 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 419 bits (1080), Expect = e-149
Identities = 101/351 (28%), Positives = 179/351 (50%), Gaps = 14/351 (3%)

Query: 7 DDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCIIWFGGESLARQLAGMLSAGLH 66
+KTE PTP ++ AR++GQ+ +S+E+ S +++ ++ + + ++ +
Sbjct: 3 GEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLML--IP 60

Query: 67 FDHRMVNDPNLILGQIILLIKAAMMALLPLIAGVVLVALISPVMLGGLIFSGKSLQPKFS 126
+ + + + ++ PL+ L+A+ S V+ G + SG++++P
Sbjct: 61 AEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIK 120

Query: 127 KLNPLPGIKRMFSAQTGAELLKAVLKSTLVGCVTGFYLWHHWPQMMRLMAESPIVAMGNA 186
K+NP+ G KR+FS ++ E LK++LK L+ + + + +++L P +
Sbjct: 121 KINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQL----PTCGIECI 176

Query: 187 LDLVGLCALLVVLGVIPMVGF------DVFFQIFSHLKKLRMSRQDIRDEFKESEGDPHV 240
L+G +L L VI VGF D F+ + ++K+L+MS+ +I+ E+KE EG P +
Sbjct: 177 TPLLG--QILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEI 234

Query: 241 KGKIRQMQRAAAQRRMMEDVPKADVIVTNPTHYSVALQYDENKMSAPKVVAKGAGLIALR 300
K K RQ + R M E+V ++ V+V NPTH ++ + Y + P V K
Sbjct: 235 KSKRRQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQT 294

Query: 301 IREIGAEHRVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWIWQLK 351
+R+I E VP L+ PLARALY A + IP + A AEVL W+ +
Sbjct: 295 VRKIAEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQN 345


73SPA1101SPA1108N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1101-111-0.0064912-dehydro-3-deoxyphosphooctonate aldolase
SPA1102-2110.012496calcium/proton antiporter
SPA1104-2111.392576hypothetical protein
SPA1105-2141.656015invasin
SPA1106-1161.877952nitrate/nitrite response regulator protein NarL
SPA11070172.000469nitrate/nitrite sensor protein NarX
SPA1108-2161.290029nitrite extrusion protein (nitrite facilitator)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1101PF03309280.046 Bvg accessory factor
		>PF03309#Bvg accessory factor

Length = 271

Score = 27.8 bits (62), Expect = 0.046
Identities = 17/84 (20%), Positives = 34/84 (40%), Gaps = 2/84 (2%)

Query: 84 LKQTFGV--KVITDVHEASQVQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQ 141
+ G + +T S V V V V+ + L+E +TG + V P+
Sbjct: 47 IDGLIGDDAERLTGASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPK 106

Query: 142 FVSPGQMGNIVDKFHEGGNDKVIL 165
V ++ N + +H+ G +++
Sbjct: 107 EVGADRIVNCLAAYHKYGTAAIVV 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1105INTIMIN2461e-74 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 246 bits (629), Expect = 1e-74
Identities = 126/444 (28%), Positives = 216/444 (48%), Gaps = 24/444 (5%)

Query: 22 SFSLSLLLLAASGTIRAQAQDPFTQNRL----PDLGMMPESHEGEKHFAEMAKAFGEASM 77
F S L L S + A N+L PD+ + + ++A A + +
Sbjct: 118 PFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDDKALNYAAQQAASLGSQL 177

Query: 78 KNNDLDTGEQARQFAFGQVRDVVSEQVNQQLESWLSAWGSASVDINVDNEGHFNGSRGSW 137
++ L+ G+ A+ A G + Q + QL++WL +G+A V++ N F+GS +
Sbjct: 178 QSRSLN-GDYAKDTALG----IAGNQASSQLQAWLQHYGTAEVNLQSGNN--FDGSSLDF 230

Query: 138 FIPLQDKQRYLTWSQLGLTQQTDGLVSNVGIGQRWAQDGWLLGYNTFYDNLLDENLQRAG 197
+P D ++ L + Q+G +N+G GQR+ +LGYN F D + R G
Sbjct: 231 LLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQDFSGDNTRLG 290

Query: 198 FGAEAWGEYLRLSANYYQPFADWQT--HTATLEQRMARGYDINAQMRLPFYQHINTSVSL 255
G E W +Y + S N Y + W + ++R A G+DI LP Y + +
Sbjct: 291 IGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLMY 350

Query: 256 EQYFGDSVDLFDSGTGYHNPVALKLGLNYTPVPLLTMTAQHKQGESGVSQNNLGLTLNYR 315
EQY+GD+V LF+S NP A +G+NYTP+PL+TM ++ G + + Y+
Sbjct: 351 EQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQ 410

Query: 316 FGVPLKKQLAASEVAQSQSLRGSRYDTPQRNSLPTMEYRQRKTLTVFLATPPWDLTPGET 375
F P +Q+ V + ++L GSRYD QRN+ +EY+++ L++ + + T T
Sbjct: 411 FDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNI-PHDINGTERST 469

Query: 376 VALKLQVRSVHGIRHLSWQGDTQALSLTAG----TDTRSTEGWTIIMPAWDHREGAANRW 431
++L V+S +G+ + W D AL G + ++S + + I+PA+ +G +N +
Sbjct: 470 QKIQLIVKSKYGLDRIVW--DDSALRSQGGQIQHSGSQSAQDYQAILPAY--VQGGSNVY 525

Query: 432 RLSVVVEDEKGQRVSSNEITLALT 455
+++ D G SSN + L +T
Sbjct: 526 KVTARAYDRNGN--SSNNVLLTIT 547


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1106HTHFIS726e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 72.2 bits (177), Expect = 6e-17
Identities = 33/117 (28%), Positives = 56/117 (47%), Gaps = 2/117 (1%)

Query: 7 ATILLIDDHPMLRTGVKQLVSMAPDISVVGEASNGEQGIDLAESLDPDLILLDLNMPGMN 66
ATIL+ DD +RT + Q +S A V SN + D DL++ D+ MP N
Sbjct: 4 ATILVADDDAAIRTVLNQALSRA-GYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 67 GLETLDKLREKALSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALQQA 123
+ L ++++ ++V S N + A ++GA YL K + +L+ + +A
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1107PF06580514e-09 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 51.4 bits (123), Expect = 4e-09
Identities = 30/123 (24%), Positives = 54/123 (43%), Gaps = 17/123 (13%)

Query: 473 SARFGFTVKLDYQLPPRL----VPSHQAIHLLQIAREALSNALKH-----SHADDVVVTV 523
S +F ++ + Q+ P + VP L+Q E N +KH +++
Sbjct: 233 SIQFEDRLQFENQINPAIMDVQVPPM----LVQTLVE---NGIKHGIAQLPQGGKILLKG 285

Query: 524 TQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRAQSLRG-DCQVRRRETGGTEVTVT 582
T+ V L+V++ G +N + S G+ +R+R Q L G + Q++ E G +
Sbjct: 286 TKDNGTVTLEVENTGSLALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMV 345

Query: 583 FIP 585
IP
Sbjct: 346 LIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1108TCRTETB300.025 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.8 bits (67), Expect = 0.025
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 1/58 (1%)

Query: 128 TPFSTFIIISLLCGFAGANF-ASSMANISFFFPKQKQGGALGLNGGLGNMGVSVMQLV 184
+ FS I+ + G A F A M ++ + PK+ +G A GL G + MG V +
Sbjct: 101 SFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAI 158


74SPA1425SPA1435N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1425019-4.860052integral membrane transport protein
SPA1426022-6.559204cyclopropane-fatty-acyl-phospholipid synthase
SPA1427127-7.576303riboflavin synthase subunit alpha
SPA1428334-8.218265hypothetical protein
SPA1431545-11.122350**type III secretion protein
SPA1432544-10.195947type III secretion protein
SPA1433341-6.802426type III secretion protein
SPA1434235-6.615353type III secretion protein
SPA1435235-6.460463type III secretion protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1425TCRTETB763e-17 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 76.1 bits (187), Expect = 3e-17
Identities = 48/194 (24%), Positives = 84/194 (43%), Gaps = 3/194 (1%)

Query: 8 LVWLAGLSVLGFLATDMYLPAFAAIQADLQTPAAAVSASLSLFLAGFAVAQLLWGPLSDR 67
L+WL LS L + + I D P A+ + + F+ F++ ++G LSD+
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 68 YGRKPILLLGLSIFALGSLGMLWVESAAALLTL-RFVQAVGVCAATVIWQALVTDYYPSQ 126
G K +LL G+ I GS+ S +LL + RF+Q G A + +V Y P +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 127 KINRIFATIMPLVGLSPALAPLLGSWILTHFSWQAIFATLFVITLLLMLPALRLKPSVKA 186
+ F I +V + + P +G I + W + L + ++ +P L +
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPMITIITVPFLMKLLKKEV 193

Query: 187 RTEGQDKLTFATLL 200
R +G + L+
Sbjct: 194 RIKGHFDIKGIILM 207


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1431TYPE3IMSPROT386e-136 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 386 bits (992), Expect = e-136
Identities = 125/350 (35%), Positives = 203/350 (58%), Gaps = 4/350 (1%)

Query: 2 SEKTEQPTEKKLRDGRKEGQVVKSIEITSLFQLIALYLYFHFFTEKMILILIESITFTLQ 61
EKTEQPT KK+RD RK+GQV KS E+ S ++AL ++ + + +
Sbjct: 3 GEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAE 62

Query: 62 LVNKPFSYALTQL-SHALIESLTSALLFLGAGVIVATVGSVFLQVGVVIASKAIGFKSEH 120
PFS AL+ + + L+E L ++A + S +Q G +I+ +AI +
Sbjct: 63 QSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMA-IASHVVQYGFLISGEAIKPDIKK 121

Query: 121 INPVSNFKQIFSLHSVVELCKSSLKVIMLSLIFAFFFYYYASTFRALPYCGLACGVLVVS 180
INP+ K+IFS+ S+VE KS LKV++LS++ T LP CG+ C ++
Sbjct: 122 INPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLG 181

Query: 181 SLIKWLWVGVMAFYIVVGILDYSFQYYKIRKDLKMSKDDVKQEHKDLEGDPQMKTRRREM 240
+++ L V ++V+ I DY+F+YY+ K+LKMSKD++K+E+K++EG P++K++RR+
Sbjct: 182 QILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQF 241

Query: 241 QSEIQSGSLAQSVKQSVAVVRNPTHIAVCLGYHPTDMPIPRVLEKGSDAQANYIVNIAER 300
EIQS ++ ++VK+S VV NPTHIA+ + Y + P+P V K +DAQ + IAE
Sbjct: 242 HQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEE 301

Query: 301 NCIPVVENVELARSLFFEVERGDKIPETLFEPVAALLRMVMK--IDYAHS 348
+P+++ + LAR+L+++ IP E A +LR + + I+ HS
Sbjct: 302 EGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNIEKQHS 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1432TYPE3IMRPROT1644e-52 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 164 bits (417), Expect = 4e-52
Identities = 55/229 (24%), Positives = 100/229 (43%), Gaps = 5/229 (2%)

Query: 8 WLIALAVAFIRPLSLSLLLPLLKSGSLGSAILRNGVLMSLTFPILPIIYQQKIMMHIGKD 67
WL +R L+L P+L S+ + + G+ M +TF I P + + +
Sbjct: 12 WLNLYFWPLLRVLALISTAPILSERSVPKRV-KLGLAMMITFAIAPSLPANDVPVF---S 67

Query: 68 YSWLGLVTGEVIIGFLIGFCAAVPFWAVDMAGFLLDTLRGATMGTIFNSTMEAETSLFGL 127
+ L L +++IG +GF F AV AG ++ G + T + +
Sbjct: 68 FFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLAR 127

Query: 128 LFSQFLCVIFFISGGVEFILNILYESYQYLPPGRTLLFDRQFLKYIQAEWRTLYQLCISF 187
+ ++F G +++++L +++ LP G L FL +A ++ +
Sbjct: 128 IMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSL-IFLNGLML 186

Query: 188 SLPAIICMVLADLALGLLNRSAQQLNVFFFSMPLKSILVLLTLLISFPY 236
+LP I ++ +LALGLLNR A QL++F PL + + + P
Sbjct: 187 ALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPL 235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1433TYPE3IMQPROT729e-21 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 72.5 bits (178), Expect = 9e-21
Identities = 30/85 (35%), Positives = 50/85 (58%)

Query: 4 SELTQFVTQLLWIVLFTSMPVVLVASVVGVIVSLVQALTQIQDQTLQFMIKLLAIAITLM 63
+L + L++VL S +VA+++G++V L Q +TQ+Q+QTL F IKLL + + L
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 VSYPWLSGILLNYTRQIMLRIGEHG 88
+ W +LL+Y RQ++ G
Sbjct: 62 LLSGWYGEVLLSYGRQVIFLALAKG 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1434TYPE3IMPPROT2319e-80 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 231 bits (592), Expect = 9e-80
Identities = 79/215 (36%), Positives = 130/215 (60%), Gaps = 8/215 (3%)

Query: 8 LQLIGILFLLSILPLIIVMGTSFLKLAVVFSILRNALGIQQVPPNIALYGLALVLSLFIM 67
+ LI +L ++LP II GT F+K ++VF ++RNALG+QQ+P N+ L G+AL+LS+F+M
Sbjct: 5 ISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLSMFVM 64

Query: 68 GPTLLAVKERWHPVQVAGAPFWT-SEWDSKALAPYRQFLQKNSEEKEANYFRNLIKRTWP 126
P + + V + S+ + L YR +L K S+ + +F N +
Sbjct: 65 WPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLIKYSDRELVQFFENAQLKRQY 124

Query: 127 ED-------IKRKIKPDSLLILIPAFTVSQLTQAFRIGLLIYLPFLAIDLLISNILLAMG 179
+ K +I+ S+ L+PA+ +S++ AF+IG +YLPF+ +DL++S++LLA+G
Sbjct: 125 GEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVLLALG 184

Query: 180 MMMVSPMTISLPFKLLIFLLAGGWDLTLAQLVQSF 214
MMM+SP+TIS P KL++F+ GW L L+ +
Sbjct: 185 MMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQY 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1435TYPE3OMOPROT542e-10 Type III secretion system outer membrane O protein ...
		>TYPE3OMOPROT#Type III secretion system outer membrane O protein

family signature.
Length = 303

Score = 53.9 bits (129), Expect = 2e-10
Identities = 59/291 (20%), Positives = 96/291 (32%), Gaps = 33/291 (11%)

Query: 31 QYPVQQGTLFTINYHNELGRVWIAEQCWQRWCEGLIGTANRSAIDPELLYGIAEWGVAPL 90
+YP +QG ++ + WI W + A SA AE V P
Sbjct: 32 EYPTRQGMWVRLSDAEKRWSAWIKPGDWLEHVSPALAGAAVSAG--------AEHLVVPW 83

Query: 91 LQASDATLCQNEPPTSCSNLPHQLALHIKWTVEEHEFHSIIFTWPTGFLRNIVGELSAER 150
L A++ P SC L VE S + P G L +I+ +
Sbjct: 84 LAATERPFELPVPHLSCRRL----------CVENPVPGSAL---PEGKLLHIMSDRGGLW 130

Query: 151 QQIYPAPPVVVPVYLGWCQLTLIELESIEIGMG-VRIHCFGDIRLGFFAIQLPGGIYARV 209
+ P P V L IG + G I +G + L A V
Sbjct: 131 FEHLPELPAVGGGRPK----MLRWPLRFVIGSSDTQRSLLGRIGIG--DVLLIRTSRAEV 184

Query: 210 LLTEDNTMKFDELVQDIETLLASGSPMSKSDGTSSV-----ELEQIPQQVLFEIGRASLE 264
F+ + I + + + T+ L Q+P ++ F + R ++
Sbjct: 185 YCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYRKNVT 244

Query: 265 IGQLRQLKTGDVLPVGGCFAPEVTIRVNDRIIGQGELIACGNEFMVRITRW 315
+ +L + +L + V I N ++G GEL+ + V I W
Sbjct: 245 LAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEW 295


75SPA1450SPA1466N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1450332-7.004349pathogenicity island protein
SPA1451335-7.671917pathogenicity island effector protein
SPA1452434-7.685469pathogenicity island effector protein
SPA1453437-8.918023pathogenicity island effector protein
SPA1454540-9.985365Type III secretion system chaperone protein
SPA1455743-10.940354pathogenicity island effector effector protein
SPA1456442-10.853540pathogenicity island protein
SPA1457442-11.025728secretion system protein
SPA1458439-9.834355pathogenicity island protein
SPA1459233-8.092680outer membrane secretory protein
SPA1460231-7.060329pathogenicity island secreted effector protein
SPA1461028-5.767530two-component sensor kinase
SPA14620170.190781two-component response regulator
SPA14630142.180498transcriptional regulator
SPA1464-1123.093163pathogenicity island protein
SPA14650131.683158hypothetical protein
SPA14660141.328299two-component response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1450SYCDCHAPRONE775e-21 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 77.3 bits (190), Expect = 5e-21
Identities = 26/127 (20%), Positives = 49/127 (38%)

Query: 14 LKQLLSVDPETVYASGYASWQEGDYSRAVIDFSWLVMAQPWSWRAHIALAGTWMMLKEYT 73
L ++ S E +Y+ + +Q G Y A F L + + R + L + +Y
Sbjct: 28 LNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYD 87

Query: 74 TAINFYGHALMLDASHPEPVYQTGVCLKMMGEPGLAREAFQTAIKMSYADASWSEIRQNA 133
AI+ Y + ++D P + CL GE A A ++ + E+
Sbjct: 88 LAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRV 147

Query: 134 QKMVDTL 140
M++ +
Sbjct: 148 SSMLEAI 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1452PF05844290.010 YopD protein
		>PF05844#YopD protein

Length = 295

Score = 29.2 bits (65), Expect = 0.010
Identities = 29/149 (19%), Positives = 48/149 (32%), Gaps = 19/149 (12%)

Query: 9 VLPAPSL-LTPSSTPSPSGEGMGTESMLLLFDDIWMKLMELAKKLRDIMRSYNVEKQRLS 67
L AP L P + E + +LL+ I K EL RD + Q+
Sbjct: 50 ELNAPRQVLDPVRMEAAGSELDSSVELLLILFRIAQKARELGVLQRDNENQAIIHAQK-- 107

Query: 68 WELQVNVLQTQMKTIDEAFRASMITAGGAMLSGVLTIGLGAVGGETGLIAGQAVGHTAGG 127
+DE + + A+++GV + VG L G+A+
Sbjct: 108 ------------AQVDEMRSGATLMIAMAVIAGVGALASAVVGSLGALKNGKAISQEK-- 153

Query: 128 VMGLGSGVAQRQSDQDKAIADLQQNGAQS 156
L + R D + L + +
Sbjct: 154 --TLQKNIDGRNELIDAKMQALGKTSDED 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1454SYCDCHAPRONE902e-25 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 89.6 bits (222), Expect = 2e-25
Identities = 39/154 (25%), Positives = 67/154 (43%), Gaps = 7/154 (4%)

Query: 6 TLQQAHDTMRFFRRGGSLRMLL---DDDVTQPLNTVYRYAMQLMEVKEFAGAARLFQLLT 62
T + F + GG++ ML D L +Y A + ++ A ++FQ L
Sbjct: 8 TQEYQLAMESFLKGGGTIAMLNEISSDT----LEQLYSLAFNQYQSGKYEDAHKVFQALC 63

Query: 63 IYDAWSFDYWFRLGECCQAQKHWGEAIYAYGRAAQIKIDAPQAPWAAAECYLACDNVCYA 122
+ D + ++ LG C QA + AI++Y A + I P+ P+ AAEC L + A
Sbjct: 64 VLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEA 123

Query: 123 IKALKAVVRICGEVSEHQILRLRAEKMLQQLSDR 156
L + + +E + L R ML+ + +
Sbjct: 124 ESGLFLAQELIADKTEFKELSTRVSSMLEAIKLK 157


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1455LIPPROTEIN48270.047 Mycoplasma P48 major surface lipoprotein signature.
		>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature.

Length = 428

Score = 27.3 bits (60), Expect = 0.047
Identities = 15/44 (34%), Positives = 22/44 (50%)

Query: 78 SNEMDEVIAKAAKGDAKTKEEVPEDVIKYMRDNGILIDGMTIDD 121
+ E I K K +E+PED +KY+ + L DG ID+
Sbjct: 368 NTEEQAKINNKIKEAIKMFKELPEDFVKYINSDKALKDGNKIDN 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1459TYPE3OMGPROT5810.0 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 581 bits (1499), Expect = 0.0
Identities = 157/500 (31%), Positives = 260/500 (52%), Gaps = 15/500 (3%)

Query: 11 LLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLITATFSGKIPP 70
LL + + + + EL W + A+ L ++L NYD + +S I SG+
Sbjct: 17 LLLLSSYSWAQELDWLPIPYVYVAKGESLRDLLTDFGANYDATVVVSDKINDKVSGQFEH 76

Query: 71 GPPVDILNNLAAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIHYLRSQNILSS 130
P D L ++A+ Y+L+ ++DG++LY++ S + ++I L+ I
Sbjct: 77 DNPQDFLQHIASLYNLVWYYDGNVLYIFKNSEVASRLIRLQESEAAELKQALQRSGIWE- 135

Query: 131 PGCEVKEITGTKAVEVSGVPSCLTRISQLASVLDNALIKR--KDSAVSVSIYTLKYATAM 188
P + + V VSG P L + Q A+ L+ R K A+++ I+ LKYA+A
Sbjct: 136 PRFGWRPDASNRLVYVSGPPRYLELVEQTAAALEQQTQIRSEKTGALAIEIFPLKYASAS 195

Query: 189 DTQYQYRDQSVVVPGVVSVL-REMSKTSVPASSTTN-----GSPATQALPMFAADPRQNA 242
D YRD V PGV ++L R +S ++ + N + A ADP NA
Sbjct: 196 DRTIHYRDDEVAAPGVATILQRVLSDATIQQVTVDNQRIPQAATRASAQARVEADPSLNA 255

Query: 243 VIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGG---- 298
+IVRD M Y++LI LD+ IE+++ I+D+NA + +LG+DW + G
Sbjct: 256 IIVRDSPERMPMYQRLIHALDKPSARIEVALSIVDINADQLTELGVDWRVGIRTGNNHQV 315

Query: 299 --KKIAFNTGLNDGGASGFSTVISDTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVL 356
K + + GA G + R+N LE A V+S+P+++T N QAV+
Sbjct: 316 VIKTTGDQSNIASNGALGSLVDARGLDYLLARVNLLENEGSAQVVSRPTLLTQENAQAVI 375

Query: 357 DKNITFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIMLNLNIQDGQQSDTQSET 416
D + T+Y K+ G++VA+L+ IT G++LR+TPR+L +I LNL+I+DG Q S
Sbjct: 376 DHSETYYVKVTGKEVAELKGITYGTMLRMTPRVLTQGDKSEISLNLHIEDGNQKPNSSGI 435

Query: 417 DPLPEVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQV 476
+ +P + + + + A + GQSL++GG + + + +K+PLLGDIP +G LFR +
Sbjct: 436 EGIPTISRTVVDTVARVGHGQSLIIGGIYRDELSVALSKVPLLGDIPYIGALFRRKSELT 495

Query: 477 HSVIRLFLIKASVVNNGISH 496
+RLF+I+ +++ GI+H
Sbjct: 496 RRTVRLFIIEPRIIDEGIAH 515


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1461HTHFIS681e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.5 bits (165), Expect = 1e-13
Identities = 31/156 (19%), Positives = 57/156 (36%), Gaps = 13/156 (8%)

Query: 691 ILLVDDADINRDIIGKMLVSQGQHVTIAASSNEALTLSQQQRFDLVLIDIRMPEIDGIEC 750
IL+ DD R ++ + L G V I +++ DLV+ D+ MP+ + +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 751 VQLWHDEPNNLDPDCMFVALSASVATEDIHRCKKNGIHHYITKPVTLATLARYISIAAEY 810
+ PD + +SA + + G + Y+ KP L L
Sbjct: 66 LP----RIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG-------- 113

Query: 811 QLLRNIELQEQDPSRCSALLAT-DDMVINSKIFQSL 845
+ R + ++ PS+ +V S Q +
Sbjct: 114 IIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEI 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1462HTHFIS667e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 7e-15
Identities = 28/119 (23%), Positives = 50/119 (42%), Gaps = 2/119 (1%)

Query: 1 MKEYKILLVDDHEIIINGIMNALLPWPHFKIVEHVKNGLEVYNACCAYEPDILILDLSLP 60
M IL+ DD I + AL + V N ++ A + D+++ D+ +P
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGY--DVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 GINGLDIIPQLHQRWPAMNILVYTAYQQEYMTIKTLAAGANGYVLKSSSQQVLLAALQT 119
N D++P++ + P + +LV +A IK GA Y+ K L+ +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1466HTHFIS842e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 84.5 bits (209), Expect = 2e-21
Identities = 31/127 (24%), Positives = 56/127 (44%)

Query: 2 ATIHLLDDDTAVTNACAFLLESLGYDVKCWTQGADFLAQASLYQAGVVLLDMRMPVLDGQ 61
ATI + DDD A+ L GYDV+ + A + +V+ D+ MP +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 GVHDALRQCGSTLAVVFLTGHGDVPMAVEQMKRGAVDFLQKPVSVKPLQAALERALTVSS 121
+ +++ L V+ ++ A++ ++GA D+L KP + L + RAL
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 122 AAVARRE 128
++ E
Sbjct: 124 RRPSKLE 130


76SPA1666SPA1675N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA16661152.473201ribonuclease E
SPA1667-1141.395952flagellar hook-associated protein 3
SPA1668-1142.344374flagellar hook-associated protein 1
SPA1669-1163.502418flagellar protein FlgJ
SPA16701143.279424flagellar P-ring protein
SPA16712152.895768flagellar L-ring protein
SPA16722152.840717flagellar basal-body rod protein FlgG (distal
SPA16732123.012563flagellar basal-body rod protein FlgF
SPA16741131.722968flagellar hook protein FlgE
SPA16750171.468451flagellar hook formation protein FlgD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1666IGASERPTASE569e-10 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 55.8 bits (134), Expect = 9e-10
Identities = 50/259 (19%), Positives = 93/259 (35%), Gaps = 26/259 (10%)

Query: 513 PSEEEYAERKRPEQPALATFAMPDVPPAPTPVEPAVSVATAKKDNVAAEQPAQPGLFSRF 572
P E+ + DVP P+ + A+ D A P P S
Sbjct: 983 PEVEKRNQTVDTTNITTPNNIQADVPSVPSN-----NEEIARVDE-APVPPPAPATPSET 1036

Query: 573 LNALKQLFSGEETKTVETAAPKAEEKAERQQDRRKPRQNNRRDRNERRDTRDNRAGRDGG 632
S +E+KTVE A E + ++ K ++N + +T+ N + G
Sbjct: 1037 TE-TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKA-----NTQTNEVAQSGS 1090

Query: 633 ESRDDNRRNRRQAQQQNAEAR---DTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQA 689
E+++ ++ E + +T + + KV + Q +P++E+S A
Sbjct: 1091 ETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS----QVSPKQEQSETVQPQAEPA 1146

Query: 690 QQEVKALNREEQPVQETEQEERVQQVQPRRKQRQLNQKVRFTNSTVVETVDTPVVVDEPR 749
++ +N +E Q + QP ++ N + T ST V T ++ V E
Sbjct: 1147 RENDPTVNIKEPQSQTNTTAD---TEQPAKETSS-NVEQPVTESTTVNTGNSVVENPENT 1202

Query: 750 PVENVEQPVPAPRTELAKV 768
+ P +E +
Sbjct: 1203 TPATTQ---PTVNSESSNK 1218



Score = 38.5 bits (89), Expect = 2e-04
Identities = 51/372 (13%), Positives = 88/372 (23%), Gaps = 47/372 (12%)

Query: 630 DGGESRDDNRRNRRQAQQQNAEARDTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQA 689
D G + R + N E Q + T + Q S +
Sbjct: 963 DLGAWKYKLRNVNGRYDLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDE 1022

Query: 690 QQEVKALNREEQPVQETEQEERVQQVQPRRKQRQLNQKVRFTNSTVVETVDTPVVVDEPR 749
ET E Q+ + K Q + N V + V +
Sbjct: 1023 APVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKE-AKSNVKANTQ 1081

Query: 750 PVENVEQPVPAPRTELAKVDLPVVADIAPEQDDSVEPRDNTGMPRRSRRSPRHLRVSGQR 809
E + T+ + A + E+ VE +++ P+ +
Sbjct: 1082 TNEVAQSGSETKETQTTETKET--ATVEKEEKAKVETE-------KTQEVPKVTSQVSPK 1132

Query: 810 RRRYRDERYPTQSPMPLTVACASPEMASGKVWIRYPIVRPQETQVVEEQREADLALPQPV 869
+ + + + P V +E Q + AD P
Sbjct: 1133 QEQSETVQPQAEPARE-----------------NDPTVNIKEPQS-QTNTTADTEQPAKE 1174

Query: 870 VAEPQVIAATVALEPQASVQAVENVAVEPQTVAEPQAPEVVKVETTHPEVIAAPVDEQPQ 929
Q V + + PE TT P V + ++
Sbjct: 1175 T-------------SSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKN 1221

Query: 930 LIAESDTPEAQEVIA------DAEPVAETADASITVAENVADVVVVEPEEETKAEAAVVE 983
S V D VA S ++D AV +
Sbjct: 1222 RHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQ 1281

Query: 984 HTAEETVIAPAQ 995
H ++ + Q
Sbjct: 1282 HISQLEMNNEGQ 1293


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1667FLAGELLIN414e-06 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 41.2 bits (96), Expect = 4e-06
Identities = 30/138 (21%), Positives = 59/138 (42%)

Query: 1 MRISTQMMYEQNMSGITNSQAEWMKLGEQMSTGKRVTNPSDDPIAASQAVVLSQAQAQNS 60
I+T + + + SQ+ E++S+G R+ + DD + A + +
Sbjct: 2 QVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLT 61

Query: 61 QYALARTFATQKVSLEESVLSQVTTAIQTAQEKIVYAGNGTLSDDDRASLATDLQGIRDQ 120
Q + E L+++ +Q +E V A NGT SD D S+ ++Q ++
Sbjct: 62 QASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEE 121

Query: 121 LMNLANSTDGNGRYIFAG 138
+ ++N T NG + +
Sbjct: 122 IDRVSNQTQFNGVKVLSQ 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1668FLGHOOKAP16640.0 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 664 bits (1714), Expect = 0.0
Identities = 438/553 (79%), Positives = 487/553 (88%), Gaps = 8/553 (1%)

Query: 2 SSLINHAMSGLNAAQAALNTVSNNINNYNVAGYTRQTTILAQANSTLGAGGWIGNGVYVS 61
SSLIN+AMSGLNAAQAALNT SNNI++YNVAGYTRQTTI+AQANSTLGAGGW+GNGVYVS
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVS 60

Query: 62 GVQREYDAFITNQLRGAQNQSSGLTTRYEQMSKIDNLLADKSSSLSGSLQSFFTSLQTLV 121
GVQREYDAFITNQLR AQ QSSGLT RYEQMSKIDN+L+ +SSL+ +Q FFTSLQTLV
Sbjct: 61 GVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLV 120

Query: 122 SNAEDPAARQALIGKAEGLVNQFKTTDQYLRDQDKQVNIAIGSSVAQINNYAKQIANLND 181
SNAEDPAARQALIGK+EGLVNQFKTTDQYLRDQDKQVNIAIG+SV QINNYAKQIA+LND
Sbjct: 121 SNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLND 180

Query: 182 QISRMTGVGAGASPNDLLDQRDQLVSELNKIVGVEVSVQDGGTYNLTMANGYTLVQGSTA 241
QISR+TGVGAGASPN+LLDQRDQLVSELN+IVGVEVSVQDGGTYN+TMANGY+LVQGSTA
Sbjct: 181 QISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTA 240

Query: 242 RQLAAVPSSADPTRTTVAYVDEAAGNIEIPEKLLNTGSLGGLLTFRSQDLDQTRNTLGQL 301
RQLAAVPSSADP+RTTVAYVD AGNIEIPEKLLNTGSLGG+LTFRSQDLDQTRNTLGQL
Sbjct: 241 RQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQL 300

Query: 302 ALAFADAFNAQHTKGYDADGNKGKDFFSIGSPVVYSNSNNADKTVSLTAKVVDSTKVQAT 361
ALAFA+AFN QH G+DA+G+ G+DFF+IG P V N+ N V++ A V D++ V AT
Sbjct: 301 ALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGD-VAIGATVTDASAVLAT 359

Query: 362 DYKIVFDGTDWQVTRTADNTTFTATKDADGKLEIDGLKVTVGTGAQKNDSFLLKPVSNAI 421
DYKI FD WQVTR A NTTFT T DA+GK+ DGL++T NDSF LKPVS+AI
Sbjct: 360 DYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAI 419

Query: 422 VDMNVKVTNEAEIAMASESKLDPDVDTGDSDNRNGQALLDLQ-NSNVVGGNKTFNDAYAT 480
V+M+V +T+EA+IAMASE D GDSDNRNGQALLDLQ NS VGG K+FNDAYA+
Sbjct: 420 VNMDVLITDEAKIAMASEE------DAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYAS 473

Query: 481 LVSDVGNKTSTLKTSSTTQANVVKQLYKQQQSVSGVNLDEEYGNLQRYQQYYLANAQVLQ 540
LVSD+GNKT+TLKTSS TQ NVV QL QQQS+SGVNLDEEYGNLQR+QQYYLANAQVLQ
Sbjct: 474 LVSDIGNKTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQ 533

Query: 541 TANALFDALLNIR 553
TANA+FDAL+NIR
Sbjct: 534 TANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1669FLGFLGJ4990.0 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 499 bits (1285), Expect = 0.0
Identities = 263/316 (83%), Positives = 289/316 (91%), Gaps = 3/316 (0%)

Query: 1 MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDG 60
MI D KLLASAAWDAQSLNELKAKAG+DPAANIRPVARQVEGMFVQMMLKSMR+ALPKDG
Sbjct: 1 MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG 60

Query: 61 LFSSDQTRLYTSMYDQQIAQQMTAGKGLGLADMMVKQMTGGQTMPADDAPQVPLKFSLET 120
LFSS+ TRLYTSMYDQQIAQQMTAGKGLGLA+MMVKQMT Q +P + P P+KF LET
Sbjct: 61 LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET 120

Query: 121 VNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLSLPARLASEQSGVPHHLILAQ 180
V YQNQAL+QLV+KA+P+ D S L GDSK FLA+LSLPA+LAS+QSGVPHHLILAQ
Sbjct: 121 VVRYQNQALSQLVQKAVPRNYDDS---LPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQ 177

Query: 181 AALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS 240
AALESGWGQRQI RENGEPSYN+FGVKA+ +WKGPVTEITTTEYENGEAKKVKAKFRVYS
Sbjct: 178 AALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYS 237

Query: 241 SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAM 300
SYLEALSDYV LLTRNPRYAAVTTAA+AEQGA ALQ+AGYATDP+YARKLT+MIQQ+K++
Sbjct: 238 SYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKSI 297

Query: 301 SEKVSKTYSANLDNLF 316
S+KVSKTYS N+DNLF
Sbjct: 298 SDKVSKTYSMNIDNLF 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1670FLGPRINGFLGI429e-153 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 429 bits (1104), Expect = e-153
Identities = 153/362 (42%), Positives = 215/362 (59%), Gaps = 9/362 (2%)

Query: 5 LAGIVLALVATLAHAERIRDLTSVQGVRENSLIGYGLVVGLDGTGDQTTQTPFTTQTLNN 64
A L+ A RI+D+ S+Q R+N LIGYGLVVGL GTGD +PFT Q++
Sbjct: 14 SALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRA 73

Query: 65 MLSQLGITVPTGTNMQLKNVAAVMVTASYPPFARQGQTIDVVVSSMGNAKSLRGGTLLMT 124
ML LGIT G + KN+AAVMVTA+ PPFA G +DV VSS+G+A SLRGG L+MT
Sbjct: 74 MLQNLGITTQGGQS-NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMT 132

Query: 125 PLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQLNGGRITNGAIIERELPTQFGAGNT 184
L G D Q+YA+AQG ++V G A +++ R+ NGAIIERELP++F
Sbjct: 133 SLSGADGQIYAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVN 192

Query: 185 INLQLNDEDFTMAQQITDAINRAR----GYGSATALDARTVQVRVPSGNSSQVRFLADIQ 240
+ LQL + DF+ A ++ D +N G A D++ + V+ P + R +A+I+
Sbjct: 193 LVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPRV-ADLTRLMAEIE 251

Query: 241 NMEVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQLNVNQPNTPFGGG 300
N+ V T AKVVIN RTG++V+ +V + AV+ G L+V V V QP PF G
Sbjct: 252 NLTVE-TDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQP-APFSRG 309

Query: 301 QTVVTPQTQIDLRQSGGSLQSVRSSANLNSVVRALNALGATPMDLMSILQSMQSAGCLRA 360
QT V PQT I Q G + ++ +L ++V LN++G +++ILQ ++SAG L+A
Sbjct: 310 QTAVQPQTDIMAMQEGSKV-AIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQA 368

Query: 361 KL 362
+L
Sbjct: 369 EL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1671FLGLRINGFLGH353e-127 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 353 bits (908), Expect = e-127
Identities = 211/232 (90%), Positives = 223/232 (96%)

Query: 1 MQKYALHAYPVMALMVATLTGCAWIPAKPLVQGATTAQPIPGPVPVANGSIFQSAQPINY 60
MQK A H Y + +L+V +LTGCAWIP+ PLVQGAT+AQP+PGP PVANGSIFQSAQPINY
Sbjct: 1 MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY 60

Query: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTSFGFDTVPRYLQGLFGNS 120
GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT+FGFDTVPRYLQGLFGN+
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA 120

Query: 121 RADMEASGGNSFNGKGGANASNTFSGTLTVTVDQVLANGNLHVVGEKQIAINQGTEFIRF 180
RAD+EASGGN+FNGKGGANASNTFSGTLTVTVDQVL NGNLHVVGEKQIAINQGTEFIRF
Sbjct: 121 RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF 180

Query: 181 SGVVNPRTISGSNSVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232
SGVVNPRTISGSN+VPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM
Sbjct: 181 SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1672FLGHOOKAP1444e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 43.8 bits (103), Expect = 4e-07
Identities = 18/81 (22%), Positives = 36/81 (44%), Gaps = 14/81 (17%)

Query: 3 SSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDLLYQTIRQPGAQSSEQTTL 62
S + A +GL+A Q ++ +NN+++ + G+ RQ + + +TL
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI--------------MAQANSTL 47

Query: 63 PSGLQIGTGVRPVATERLHSQ 83
+G +G GV +R +
Sbjct: 48 GAGGWVGNGVYVSGVQREYDA 68



Score = 41.1 bits (96), Expect = 3e-06
Identities = 11/41 (26%), Positives = 21/41 (51%)

Query: 220 ETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL 260
S VN+ EE N+ + Q+ Y N++ + T + + L +
Sbjct: 505 SISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1674FLGHOOKAP1417e-06 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 41.1 bits (96), Expect = 7e-06
Identities = 17/48 (35%), Positives = 29/48 (60%)

Query: 356 LTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQILNTLVNLR 403
L+N S V+L +E N+ Q+ Y +NAQ ++T + I + L+N+R
Sbjct: 499 LSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 37.2 bits (86), Expect = 9e-05
Identities = 22/60 (36%), Positives = 31/60 (51%), Gaps = 4/60 (6%)

Query: 2 SFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFAD----MFAGSKVGLGVKVAGI 57
+ A+SGLNAA L+ NNI++ G+ T A + AG VG GV V+G+
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1675SYCECHAPRONE290.010 Gram-negative bacterial type III secretion SycE cha...
		>SYCECHAPRONE#Gram-negative bacterial type III secretion SycE

chaperone signature.
Length = 130

Score = 28.5 bits (63), Expect = 0.010
Identities = 16/34 (47%), Positives = 20/34 (58%), Gaps = 2/34 (5%)

Query: 44 LKNQDPTNPLQNNELTTQLAQISTVSGIEKLNTT 77
L N+ P N L NN L TQL + V G E+L T+
Sbjct: 89 LWNRQPLNSLDNNSLYTQLEML--VQGAERLQTS 120


77SPA1754SPA1760N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1754125-4.992403response regulator
SPA1755328-6.640745histidine kinase
SPA1757338-10.277209hypothetical protein
SPA1758338-10.119887hypothetical protein
SPA1759333-8.815818cell invasion protein
SPA1760332-9.156860cell invasion protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1754HTHFIS831e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 83.0 bits (205), Expect = 1e-20
Identities = 30/117 (25%), Positives = 56/117 (47%), Gaps = 1/117 (0%)

Query: 2 KILLIEDNQKTIEWVRQGLTEAGYMVDYACDGRDGLHLALQEHYSLIILDIMLPGLDGWQ 61
IL+ +D+ + Q L+ AGY V + L++ D+++P + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLRALRTAHQS-PVICLTARDSVEDRVKGLEAGANDYLVKPFSFAELLARVRAQLRQ 117
+L ++ A PV+ ++A+++ +K E GA DYL KPF EL+ + L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1755PF06580340.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 33.7 bits (77), Expect = 0.001
Identities = 18/102 (17%), Positives = 38/102 (37%), Gaps = 15/102 (14%)

Query: 348 ILLQRVLSNLLTNAIRYSDENAVIRIESAYDDNVAEIRVANPGSHPADADKLFRRFWRGD 407
+L+Q ++ N + + I + I ++ D+ + V N GS K
Sbjct: 258 MLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE-------- 309

Query: 408 NARHTAGFGLGLSLVNA-IALLHGGSASYRYADEHNIFSVRL 448
G GL V + +L+G A + +++ + +
Sbjct: 310 ------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMV 345


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1759TYPE3OMBPROT6550.0 Type III secretion system outer membrane B protein ...
		>TYPE3OMBPROT#Type III secretion system outer membrane B protein

family signature.
Length = 538

Score = 655 bits (1692), Expect = 0.0
Identities = 186/396 (46%), Positives = 253/396 (63%), Gaps = 5/396 (1%)

Query: 166 LNNQPWQTIKNTLTHNGHHYTNTQLPAAEMKIGAKDIFPSAYQGKGVCSWDTKNIHHANN 225
LNN+ W + ++H+G +Y PA+ MKIG K+IF Y GKG+C T+ H N
Sbjct: 146 LNNKNWGPVNKNISHHGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIAN 205

Query: 226 LWMSTVSVHEDGKDKTLFCGIRHGVLSPYH-EKDPLLRQVGAENKAKEVLTAALYSKPEL 284
+W+S V V ++GK+ +F GIRHGV+S Y +K+ R V A NKA+E+++AALYS+PEL
Sbjct: 206 MWLSKV-VDDEGKE--IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPEL 262

Query: 285 LNRALEGEAVSLKLVSVGLLTASNIFGKEGTMVEDQMRAWQSL-TQPGKMIHLKIRNKDG 343
L++AL G+ V LK+VS LLT +++ G E +M++DQ+ A + L ++ G+ L IRN DG
Sbjct: 263 LSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDG 322

Query: 344 DLQTVKIKPDVAAFNVGVNELALKLGFGLKASDRYNAEALHQLLGNDLRPEARPGGWVGE 403
L+ V + V FN GVNELALK+G G + D+ N E++ LLG++ GGW E
Sbjct: 323 LLKEVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAE 382

Query: 404 WLAQYPDNYEVVNTLARQIKDIWKNNQHHKDGGEPYKLAQRLAMLAHEIDAVPAWNCKSG 463
+ + P V LA QIK+I D GEPYKL+QR+ +LA+ I AVP WNCKSG
Sbjct: 383 AIEKNPPCKNDVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSG 442

Query: 464 KDRTGMMDSEIKREHISLHQTHMLSAPGSLPDSGGQKIFQKVLLNSGNLEIQKQNTGGAG 523
KDRTGM D+EIKRE I H+T S S S +++F +L+NSGN+EIQ+ NTG G
Sbjct: 443 KDRTGMQDAEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPG 502

Query: 524 NKVMKNLSPEVLNLSYQKRVGDENIWQSVKGISSLI 559
NKVMK L L LSY +R+GD IW VKG SS +
Sbjct: 503 NKVMKKLPLSSLELSYSERIGDSKIWNMVKGYSSFV 538


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1760PF078241651e-56 Type III secretion chaperone
		>PF07824#Type III secretion chaperone

Length = 120

Score = 165 bits (419), Expect = 1e-56
Identities = 33/114 (28%), Positives = 63/114 (55%), Gaps = 1/114 (0%)

Query: 1 MESLLNRLYDALGLDAPE-DEPLLIIDDGIQVYFNESDHTLEMCCPFMPLPDDILTLQHF 59
ME L + + ALG+ + + D+ +++DD + +Y + ++ + CPF LP++I L +
Sbjct: 1 MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLLCPFCALPENINDLIYA 60

Query: 60 LRLNYTSAVTIGADADNTALVALYRLPQTSTEEEALTGFELFISNVKQLKEHYA 113
L LNY+ + + D + +L+A L + E+ E +IS V+ LK+ +A
Sbjct: 61 LSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYYISRVRWLKDEFA 114


78SPA1866SPA1872N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA18661152.887533hypothetical protein
SPA18670141.196144oxidoreductase
SPA1868-1141.197159N-acetylmuramoyl-L-alanine amidase
SPA18690130.285485hypothetical protein
SPA1870-113-0.688474lipoprotein
SPA1871-313-1.418516arginine transport ATP-binding protein ArtP
SPA1872-212-2.600501arginine/ornithine ABC transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1866NUCEPIMERASE552e-10 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 55.2 bits (133), Expect = 2e-10
Identities = 30/125 (24%), Positives = 49/125 (39%), Gaps = 17/125 (13%)

Query: 4 RILVLGASGYIGQHLVFALSQQGHQVRA---------AARRVERLEKQRLANVSCHKVDL 54
+ LV GA+G+IG H+ L + GHQV + + RLE HK+DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 HWPENLPTLLRD--VDTVYYLVH------GMGEGGDFIAHERQAALNVRDALRQTPVKQL 106
E + L + V+ H + + LN+ + R ++ L
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 107 IFLSS 111
++ SS
Sbjct: 122 LYASS 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1867NUCEPIMERASE662e-14 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 66.3 bits (162), Expect = 2e-14
Identities = 69/370 (18%), Positives = 123/370 (33%), Gaps = 71/370 (19%)

Query: 1 MKVLVTGATSGLGRNAVEFLRNKGISVRA---------TGRNEAMGKLLEKMGAEFVHAD 51
MK LVTGA +G + + L G V +A +LL + G +F D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 52 LTELVSSQAKVMLAGIDTLWHCS-------SFTSPWGTQQAFDLANVRATRRLGEWAVAW 104
L + + ++ S +P A+ +N+ + E
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPH----AYADSNLTGFLNILEGCRHN 116

Query: 105 GVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAAGEEVINLLAQANPQTH-- 162
+++ ++ SS S+Y + D + +A +K A E L+A +
Sbjct: 117 KIQHLLYASSSSVYGL-NRKMPFSTDDSVDHPVSLYAATKKANE----LMAHTYSHLYGL 171

Query: 163 -FTVLRPQSLFGPHDK--VFIPRLAHMMHHYGSVLLPHGGSALVDMTYYENAIHAMWLAS 219
T LR +++GP + + + + M S+ + + G D TY ++ A+
Sbjct: 172 PATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQ 231

Query: 220 QPGCDHLPS--------------GRAYNITNGENRTLRSIVQKLIDELTIDCRIRSVPYP 265
R YNI N L +Q L D L I+ + +P
Sbjct: 232 DVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQ 291

Query: 266 MLDMIARSMERFGKKSAKEPPLTHYGVSKLNFDFTLDTTRAQDELGYQPIVTLDEGIERT 325
D+ T DT + +G+ P T+ +G++
Sbjct: 292 PGDV----------------LETS-----------ADTKALYEVIGFTPETTVKDGVKNF 324

Query: 326 AAWLRDHGNL 335
W RD +
Sbjct: 325 VNWYRDFYKV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1871PF05272300.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.006
Identities = 16/50 (32%), Positives = 22/50 (44%), Gaps = 1/50 (2%)

Query: 31 LVLLGPSGAGKSSLLRVLNLLEMPRSGTLTIAGNHFDFTKTPSDKAIREL 80
+VL G G GKS+L+ L L+ S T G D + + EL
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDF-FSDTHFDIGTGKDSYEQIAGIVAYEL 647


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1872FLGFLIH300.006 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 30.1 bits (67), Expect = 0.006
Identities = 34/119 (28%), Positives = 50/119 (42%), Gaps = 31/119 (26%)

Query: 81 FDAVMAG--MDITPEREKQVLFTTPYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQ 138
D+V+A M + E +QV+ TP DNSAL + QL Q
Sbjct: 112 LDSVIASRLMQMALEAARQVIGQTPTVDNSALI-------KQIQQL-----------LQQ 153

Query: 139 KFIMDKHPEITTVPYDSYQNAKLDLQNGRIDAVFGDTAVVTEW-LKANPKLVPVGDKVT 196
+ + P++ P DLQ R+D + G T + W L+ +P L P G KV+
Sbjct: 154 EPLFSGKPQLRVHPD--------DLQ--RVDDMLGATLSLHGWRLRGDPTLHPGGCKVS 202


79SPA1892SPA1899N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA18920111.022638hypothetical protein
SPA18930130.794577putative tetR-family transcriptional regulator
SPA1894-1110.895062hypothetical protein
SPA1895-2120.268179hypothetical protein
SPA1896-1101.067003multidrug translocase MdfA
SPA1897-1110.602758permease
SPA1898-190.475192deoxyribose operon repressor
SPA18990101.059302D-alanyl-D-alanine carboxypeptidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1892TCRTETA310.011 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 31.3 bits (71), Expect = 0.011
Identities = 21/106 (19%), Positives = 34/106 (32%), Gaps = 6/106 (5%)

Query: 394 LMIGMITFQFSNFSFGIGNAAGLLFAGIML-GFLRANHPTFG-YIPQ--GALNMVKEFGL 449
L++ + +L+ G ++ G A G YI + FG
Sbjct: 76 LLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGF 135

Query: 450 MVFMAGVGLSAGSGISNGLGAVGGQM--LIAGLVVSLVPVVICFLF 493
M G G+ AG + +G A + L + CFL
Sbjct: 136 MSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1893HTHTETR476e-09 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 47.3 bits (112), Expect = 6e-09
Identities = 17/80 (21%), Positives = 33/80 (41%)

Query: 7 RRANDPKRREKIIQATLEAVKTYGVHAVTHRKIAAIAQVPLGSMTYYFAGMDALLSEAFT 66
+ + R+ I+ L GV + + +IA A V G++ ++F L SE +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 67 LFTENMSRQYQDFFAQVTDA 86
L N+ ++ A+
Sbjct: 65 LSESNIGELELEYQAKFPGD 84


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1894TCRTETB330.002 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 32.9 bits (75), Expect = 0.002
Identities = 33/150 (22%), Positives = 65/150 (43%), Gaps = 6/150 (4%)

Query: 200 LLIGVVVLAMAFAEGSANDWL-PLLMVDGHGFSP-TSGSLIYAGFTLGMTVGRFTGGWFI 257
+IGV+ + F + + P +M D H S GS+I T+ + + + GG +
Sbjct: 258 FMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILV 317

Query: 258 DRYSRVTVVR-ASALM--GALGIGLIIFVDSDWVA-GVSVILWGLGASLGFPLTISAASD 313
DR + V+ + L ++ S ++ + +L GL + TI ++S
Sbjct: 318 DRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSL 377

Query: 314 TGPDAPTRVSVVATTGYLAFLVGPPLLGYL 343
+A +S++ T +L+ G ++G L
Sbjct: 378 KQQEAGAGMSLLNFTSFLSEGTGIAIVGGL 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1896TCRTETB432e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 42.9 bits (101), Expect = 2e-06
Identities = 66/356 (18%), Positives = 126/356 (35%), Gaps = 51/356 (14%)

Query: 48 QAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLATLLAKNIEQ 107
A +WV T+ + G + G LSD++G + ++L G++ + + +
Sbjct: 48 PASTNWVNTAFMLTFSIGTAV---YGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS 104

Query: 108 FT-FLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWVH 166
RF+QG A+ + + K L+ ++ + +GP +G H
Sbjct: 105 LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAH 164

Query: 167 VLPWEGMFILFAALAAIAFFGLQCAMPETATRRGE------------------------- 201
+ W +L + I L + + +G
Sbjct: 165 YIHW-SYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSI 223

Query: 202 ------TLSFKALGRDYRLV---------IKNRRFVAGALALGFVSLPLLAWIAQSPIII 246
LSF + R V KN F+ G L G + + +++ P ++
Sbjct: 224 SFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMM 283

Query: 247 ISGEQLSSYEYG-LLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAA 305
QLS+ E G ++ P ++I + L RR ++ +G + + A+
Sbjct: 284 KDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTAS-- 341

Query: 306 TVVSSHAYLWMTAGLSVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIF 361
+ +MT + V+ G GL+ V T+ SS + + A M +L F
Sbjct: 342 -FLLETTSWFMTIII-VFVLG-GLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSF 394


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1899BLACTAMASEA475e-08 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 46.7 bits (111), Expect = 5e-08
Identities = 49/207 (23%), Positives = 78/207 (37%), Gaps = 25/207 (12%)

Query: 1 MTQYASSLRSLAAGSVLLFLFASPVKAEEQTIAPPGVDAR-AWILMDYASGKVLAEGNAD 59
M + SL A ++ L + ASP E+ ++ + R I MD ASG+ L AD
Sbjct: 1 MRYIRLCIISLLA-TLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRAD 59

Query: 60 EKLDPASLTKIMTSYVVGQALKAGKIKLTDMVTVGKDAWATGNPALRGSSVMFLKPGDQV 119
E+ S K++ V + AG +L + + +P V D +
Sbjct: 60 ERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSP------VSEKHLADGM 113

Query: 120 SVADLNKGIIIQSGNDACIALADYVAGSQESFIGLMNAYAKRLGLTNTT---FQTVHGLD 176
+V +L I S N A L V G + A+ +++G T ++T
Sbjct: 114 TVGELCAAAITMSDNSAANLLLATVGGPAG-----LTAFLRQIGDNVTRLDRWETELNEA 168

Query: 177 APGQF---STARDMA------LLGKAL 194
PG +T MA L + L
Sbjct: 169 LPGDARDTTTPASMAATLRKLLTSQRL 195


80SPA1933SPA1938N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA1933-2152.259679ATP-dependent RNA helicase rhlE
SPA1934-2151.747323hypothetical tetR-family transcriptional
SPA1935-2162.231085HlyD-family secretion protein
SPA1936-3151.796450ABC transporter ATP-binding protein
SPA1937-1181.331992inner membrane protein
SPA1938-1170.880919inner membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1933SECA300.023 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.023
Identities = 20/67 (29%), Positives = 34/67 (50%), Gaps = 4/67 (5%)

Query: 246 QQVLVFTRTKHGANHLAEQLNKDGIRSAAIHG-NKSQGARTRALADFKSGDIRVLVATDI 304
Q VLV T + + ++ +L K GI+ ++ + A A A + + V +AT++
Sbjct: 450 QPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAA---VTIATNM 506

Query: 305 AARGLDI 311
A RG DI
Sbjct: 507 AGRGTDI 513


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1934HTHTETR662e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 65.8 bits (160), Expect = 2e-15
Identities = 32/224 (14%), Positives = 72/224 (32%), Gaps = 25/224 (11%)

Query: 6 TTTKGEQAKSQLIAAALAQFGEYGLHATT-RDIAALAGQNIAAITYYFGSKEDLYLACAQ 64
T + ++ + ++ AL F + G+ +T+ +IA AG AI ++F K DL+ +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 65 WIADFLGEKFRPHAEKAERLFSQPAPD-RDAIRELILLACKNMIMLLTQEDTVNLSKFIS 123
+GE E + P R+ + ++ L E + +F+
Sbjct: 65 LSESNIGELEL---EYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV- 120

Query: 124 REQLSPTSAYQLVHEQVIDPLHTHLTRLVAA---YTGCDANDTRMILHTHALLGEVLAFR 180
E A + + + D + L + A +I+ + ++
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM--RGYISGLM--- 175

Query: 181 LGKETILLRTGWPQFDEEKAELIYQTVTCHIDLILHGLTQRSLD 224
W + + ++ ++L
Sbjct: 176 ---------ENWLFAPQSFDLK--KEARDYVAILLEMYLLCPTL 208


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1935RTXTOXIND612e-12 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 61.0 bits (148), Expect = 2e-12
Identities = 48/286 (16%), Positives = 104/286 (36%), Gaps = 28/286 (9%)

Query: 55 ASLNVDEGDAIKAGQVLGELDHAPYENALMQAKAGVSVAQAQYDLMLAGYRDEEIAQAAA 114
NV E + L + + ++N Q + + +A+ +LA E
Sbjct: 175 YFQNVSEEEV-LRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 115 AVRQAQAAYDYAQNFYNRQQGLWKSRTISA--NDLENARSSRDQAQATLKSAQDKLSQYR 172
R + + + L + N+L +S +Q ++ + SA+++
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQL-V 292

Query: 173 TGNREQDI----AQAKASLEQAKAQLAQAQLDLQDTTLIAPANGTLLTRAV-EPGSMLNA 227
T + +I Q ++ +LA+ + Q + + AP + + V G ++
Sbjct: 293 TQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTT 352

Query: 228 GSTVLTLSLT-RPVWVRAYVDERNLSQTQPGRDILLYTDGRPDKPYH---GKIGFVSPTA 283
T++ + + V A V +++ G++ ++ + P Y GK+ ++ A
Sbjct: 353 AETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA 412

Query: 284 EFTPKTVETPDLRTDLVYRLRIIVT-------DADDALRQGMPVTV 322
D R LV+ + I + + + L GM VT
Sbjct: 413 --------IEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTA 450


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1936PF05272320.009 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 31.6 bits (71), Expect = 0.009
Identities = 22/89 (24%), Positives = 29/89 (32%), Gaps = 21/89 (23%)

Query: 294 PRFEDAFIDLLGGAGTSESPLGSILHTVEGTAGETVIEAQELTKKFGDFAATDHVNFVVQ 353
PR E + +LG P + Q + K HV V++
Sbjct: 548 PRLEKWLVHVLGKTPDDYKP-------------RRLRYLQLVGKYI----LMGHVARVME 590

Query: 354 RGEIFG----LLGPNGAGKSTTFKMMCGL 378
G F L G G GKST + GL
Sbjct: 591 PGCKFDYSVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA1938ABC2TRNSPORT461e-07 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 45.7 bits (108), Expect = 1e-07
Identities = 35/139 (25%), Positives = 60/139 (43%), Gaps = 5/139 (3%)

Query: 197 AREREQGTLDQLLVSPLTTWQIFVGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALF 256
R Q T + +L + L I +G+ A A IG+ A + + L+L
Sbjct: 92 GRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGA---GIGVVAAALGYTQWLSLL 148

Query: 257 YFTMVI--YGLSLVGFGLLISSLCATQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQN 314
Y VI GL+ G+++++L + + + P + LSG V PV+ +P+ Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 315 LTWINPIRHFTDITKQIYL 333
P+ H D+ + I L
Sbjct: 209 AARFLPLSHSIDLIRPIML 227


81SPA2136SPA2141N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2136-2134.6829882,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
SPA2137-1125.189781isochorismatase
SPA21380115.5579222,3-dihydroxybenzoate-AMP ligase
SPA21391135.615635isochorismate synthase EntC
SPA21401133.813006ferrienterobactin-binding periplasmic protein
SPA21412154.584553membrane protein p43
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2136DHBDHDRGNASE338e-120 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 338 bits (868), Expect = e-120
Identities = 104/257 (40%), Positives = 147/257 (57%), Gaps = 20/257 (7%)

Query: 9 KTVWVTGAGKGIGYATALAFVDAGARVIGFDRE---------------FTQENYPFATEV 53
K ++TGA +GIG A A GA + D E +P
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP----- 63

Query: 54 MDVADAGQVAQVCQRVLQKTPRLDVLVNAAGILRMGATDALSVDDWQQTFAVNVGGAFNL 113
DV D+ + ++ R+ ++ +D+LVN AG+LR G +LS ++W+ TF+VN G FN
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 114 FSQTMAQFRRQQGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALTVGLELAGCGVRCN 173
++ G+IVTV S+ A PR M+AY +SKAA +GLELA +RCN
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 174 VVSPGSTDTDMQRTLWVSEDAEQQRIRGFGEQFKLGIPLGKIARPQEIANTILFLASDLA 233
+VSPGST+TDMQ +LW E+ +Q I+G E FK GIPL K+A+P +IA+ +LFL S A
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 234 SHITLQDIVVDGGSTLG 250
HIT+ ++ VDGG+TLG
Sbjct: 244 GHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2137ISCHRISMTASE425e-154 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 425 bits (1095), Expect = e-154
Identities = 148/299 (49%), Positives = 192/299 (64%), Gaps = 18/299 (6%)

Query: 1 MAIPKLQSYALPTALDIPTNKVNWAFEPERAALLIHDMQDYFVSFWGRNCPMMDQVIANI 60
MAIP +Q Y +PTA D+P NKV+W +P RA LLIHDMQ+YFV + + ++ ANI
Sbjct: 1 MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI 60

Query: 61 AALRQYCKEHHIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVEALTPDEADTV 120
L+ C + IPV YTAQP Q+ +DRALL D WGPGL P ++K++ L P++ D V
Sbjct: 61 RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV 120

Query: 121 LVKWRYSAFHRSPLEQMLKDTGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD 180
L KWRYSAF R+ L +M++ GR+QLIITG+YAHIGC+ TA +AFM DIK F V DA+AD
Sbjct: 121 LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD 180

Query: 181 FSREEHLMALNYVAGRSGRVVMTESLL------PTPVPASKA-----------ALRALIL 223
FS E+H MAL Y AGR VMT+SLL P V + A +R I
Sbjct: 181 FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA 240

Query: 224 PLLDETDEPLD-DENLIDYGLDSVRMMGLAARWRKVHGDIDFVMLAKNPTIDAWWALLS 281
LL ET E + E+L+D GLDSVR+M L +WR+ ++ FV LA+ PTI+ W LL+
Sbjct: 241 ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLT 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2140FERRIBNDNGPP594e-12 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 58.8 bits (142), Expect = 4e-12
Identities = 46/210 (21%), Positives = 81/210 (38%), Gaps = 21/210 (10%)

Query: 105 EPNAETVAAQMPDLILISATGGDSALALYDQLSAIAPTLVINYDDKS-----WQSLLTQL 159
EPN E + P ++ SA G S + L+ IAP N+ D + LT++
Sbjct: 86 EPNLELLTEMKPSFMVWSAGYGPS----PEMLARIAPGRGFNFSDGKQPLAMARKSLTEM 141

Query: 160 GEITGQEKQAAARIAEFEAQLTTVKQRIALPPQPVSALVYTPAAHSANLWTPESTQGKLL 219
++ + A +A++E + ++K R L ++ P S ++L
Sbjct: 142 ADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEIL 201

Query: 220 TQVGFTLATLPQGLQTSKSQGKRHDIIQLGGENLAAGLNGESLFLFAGDNNDVAALYANP 279
+ G A + + + + LAA + + L ++ D+ AL A P
Sbjct: 202 DEYGIPNAW--------QGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATP 253

Query: 280 LLAHLPAVQNKRVYALGTETFRLDYYSATL 309
L +P V+ R + F Y ATL
Sbjct: 254 LWQAMPFVRAGRFQRVPAVWF----YGATL 279


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2141TCRTETB290.039 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.1 bits (65), Expect = 0.039
Identities = 69/394 (17%), Positives = 130/394 (32%), Gaps = 60/394 (15%)

Query: 27 FISIVSLGLLGVAVPVQIQMMTHSTWQVGLSVTLTGGAMFIGLMVGGVLADRYERKKVIL 86
F S+++ +L V++P T IG V G L+D+ K+++L
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 87 LARGTCGIGFIGLCVNALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQ 146
G + V ++A ++ G F +L ++ + +EN +
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPAL----VMVVVARYIPKENRGK 139

Query: 147 AGAITMLTVRLGSVISPMLGGILLASGGVAWNYGLAAAGTFITLLPLLTLPRLPVPPQPR 206
A + V +G + P +GG++ + W+Y L IT++ + L +L
Sbjct: 140 AFGLIGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIP--MITIITVPFLMKLLKKEVRI 195

Query: 207 ENP--------------------------FLALLAAFRFLLA------------------ 222
+ FL + +
Sbjct: 196 KGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKN 255

Query: 223 CPLIGGIALLGGLVTMASAVRVLYPALAMS--WQMSAAQIGLLYAAI-PLGAAIGALTSG 279
P + G+ L GG++ A V M Q+S A+IG + + I G
Sbjct: 256 IPFMIGV-LCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGG 314

Query: 280 QLAHSVRPGLIMLVSTVG---SFLAVGLFAIMPVWIAGVICLALFGWLSAISSLLQYTLL 336
L P ++ + SFL W +I + + G LS +++ +
Sbjct: 315 ILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVS 374

Query: 337 QTQTPENMLGRMNGLWTAQNVTGDAIGAALLGGL 370
+ + M L + + G A++GGL
Sbjct: 375 SSLKQQEAGAGM-SLLNFTSFLSEGTGIAIVGGL 407


82SPA2238SPA2247N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA22383152.788460DNA polymerase III subunits gamma and tau
SPA2239113-0.174576adenine phosphoribosyltransferase
SPA2240114-0.976765hypothetical protein
SPA2241111-0.544432primosomal replication protein N
SPA2242-110-0.373998hypothetical protein
SPA2243-110-0.670956hypothetical protein
SPA2244010-0.967157integral membrane protein AefA
SPA2245014-1.376790acrAB operon repressor
SPA2246014-1.039031acriflavin resistance protein A
SPA2247014-2.017724acriflavin resistance protein B
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2238IGASERPTASE459e-07 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 45.1 bits (106), Expect = 9e-07
Identities = 52/275 (18%), Positives = 85/275 (30%), Gaps = 34/275 (12%)

Query: 366 PEPETPRQSFAPVAPTAVMTPP--QVQQPSAP-----------APQTSPAPLPASTSQVL 412
PE E Q V T + TP Q PS P AP PAP S +
Sbjct: 983 PEVEKRNQ---TVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTET 1039

Query: 413 AARNQLQRAQGVTKTKK--SEPAAASRARPVNNSALERLASVSERVQARPAPSALETAPV 470
A N Q ++ V K ++ +E A + R + + + E A
Sbjct: 1040 VAENSKQESKTVEKNEQDATETTAQN-----------REVAKEAKSNVKANTQTNEVAQS 1088

Query: 471 KKEAYRWKATTPVVQTKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLS 530
E T +TKE K K +E EKT E K+ ++ + + V +
Sbjct: 1089 GSET----KETQTTETKETATVEKEEKAKVETEKTQE-VPKVTSQVSPKQEQSETVQPQA 1143

Query: 531 LPKLVEQVALNAWKEQNGNAVCLHLRSTQRHLNSSGAQQKLAQALSDLTGTTVELTIVED 590
P +N + Q+ + +S+ Q + + VE
Sbjct: 1144 EPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTT 1203

Query: 591 DNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT 625
T + + ++ S+ + T
Sbjct: 1204 PATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPAT 1238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2243FLGFLIH310.005 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 30.9 bits (69), Expect = 0.005
Identities = 28/63 (44%), Positives = 37/63 (58%), Gaps = 6/63 (9%)

Query: 222 AEPGALIRQLAQGAPQYKEQLMT--IAEWLEE---KGRTEGLQKGLEQGLAQGREAEARA 276
AEP +L +QLAQ Q EQ IAE ++ +G EGL +GLEQGLA+ + +A
Sbjct: 36 AEP-SLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPI 94

Query: 277 IAR 279
AR
Sbjct: 95 HAR 97


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2244CHANLCOLICIN367e-04 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 36.2 bits (83), Expect = 7e-04
Identities = 41/219 (18%), Positives = 81/219 (36%), Gaps = 18/219 (8%)

Query: 92 RQKVAQAPEKMRQ-ATAALNALSDVDNDDEMRKTLSALSLRQLELRVA--QVLDDLQNSQ 148
R ++A+A EK R+ A AA A + + + + A + RQL+L A + L L
Sbjct: 129 RLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEA 188

Query: 149 NDLAAYNSQLVSLQTQPERVQNAMYTASQQI-------QQIRNRLDGNNVGEAALRPSQQ 201
+ +L + Q++ ++ + T + ++ L G A +
Sbjct: 189 KAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYK 248

Query: 202 VLLQAQQALLNAQID--------QQRKSLEGNTVLQDTLQKQRDYVTANSNRLEHQLQLL 253
L + + L D + + G +++ QKQ NR+ + +
Sbjct: 249 ELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINADITQI 308

Query: 254 QEAVNSKRLTLTEKTAQEAISPDETARIQANPLVKQELD 292
Q+A++ A+ + + + Q N L Q D
Sbjct: 309 QKAISQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKD 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2245HTHTETR2048e-69 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 204 bits (519), Expect = 8e-69
Identities = 187/214 (87%), Positives = 199/214 (92%)

Query: 1 MARKTKQQALETRQHILDVALRLFSQQGVSATSLAEIANAAGVTRGAIYWHFKNKSDLFS 60
MARKTKQ+A ETRQHILDVALRLFSQQGVS+TSL EIA AAGVTRGAIYWHFK+KSDLFS
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EIWELSESNIGELEIEYQAKFPDDPLSVLREILVHILEATVTEERRRLLMEIIFHKCEFV 120
EIWELSESNIGELE+EYQAKFP DPLSVLREIL+H+LE+TVTEERRRLLMEIIFHKCEFV
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 121 GEMVVVQQAQRSLCLESYDRIEQTLKHCINAKMLPENLLTRRAAILMRSFISGLMENWLF 180
GEM VVQQAQR+LCLESYDRIEQTLKHCI AKMLP +L+TRRAAI+MR +ISGLMENWLF
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 181 APQSFDLKKEARAYVTILLEMYQLCPTLRASTVN 214
APQSFDLKKEAR YV ILLEMY LCPTLR N
Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPATN 214


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2246RTXTOXIND431e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 43.3 bits (102), Expect = 1e-06
Identities = 33/216 (15%), Positives = 75/216 (34%), Gaps = 27/216 (12%)

Query: 100 TYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVA 159
+ Y A +L + + ++ + Q +++ ++ L +Q T +
Sbjct: 256 EQENKYVEAVNELR--VYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGL 313

Query: 160 AKAAVETARINLAYTKVTSPISGRIGKSSV-TEGALVQNGQASALATVQQLDPIYVDVTQ 218
+ + + +P+S ++ + V TEG +V + + V + D + V
Sbjct: 314 LTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAET-LMVIVPEDDTLEVTALV 372

Query: 219 SSNDFLRLKQELA------------NGSLKQENGKAKVDLVTSDGIKFPQSGTLEFSDVT 266
+ D + G L KV + D I+ + G + ++
Sbjct: 373 QNKDIGFINVGQNAIIKVEAFPYTRYGYL-----VGKVKNINLDAIEDQRLGLVFNVIIS 427

Query: 267 VDQTTGSITLRAIFPNPDHTLLPGMFVRARLQEGTK 302
+++ S + I L GM V A ++ G +
Sbjct: 428 IEENCLSTGNKNIP------LSSGMAVTAEIKTGMR 457



Score = 32.9 bits (75), Expect = 0.002
Identities = 24/133 (18%), Positives = 45/133 (33%), Gaps = 10/133 (7%)

Query: 49 PLQITTELPGR-TVAYRIAEVRPQVSGIILKRNFV-EGSDIEAGVSLYQIDP-------A 99
++I G+ T + R E++P + I+ K V EG + G L ++
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIV-KEIIVKEGESVRKGDVLLKLTALGAEADTL 137

Query: 100 TYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVA 159
Q++ A+ + + Q + EL KL Y ++ L
Sbjct: 138 KTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFST 197

Query: 160 AKAAVETARINLA 172
+ +NL
Sbjct: 198 WQNQKYQKELNLD 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2247ACRIFLAVINRP13660.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1366 bits (3538), Expect = 0.0
Identities = 808/1033 (78%), Positives = 916/1033 (88%), Gaps = 1/1033 (0%)

Query: 1 MPNFFIDRPIFAWVIAIIIMLAGGLAIFKLPVAQYPTIALPAVTISATYPGADAKTVQDT 60
M NFFI RPIFAWV+AII+M+AG LAI +LPVAQYPTIA PAV++SA YPGADA+TVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADIAQVQVQNKLQLAMPLLPQ 120
VTQVIEQNMNGIDNLMYMSS SDS G+V ITLTF+SGTD DIAQVQVQNKLQLA PLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAANMKDPISRTSGVGDVQLFGS 180
EVQQQG+SVEKSSSS+LMV G ++ + TQ+DISDYVA+N+KD +SR +GVGDVQLFG+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWMNPTELTKYQLTPVDVINAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRL 240
QYAMRIW++ L KY+LTPVDVIN +K QN Q+AAGQLGGTP + GQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 TSTDEFGKILLKVNQDGSQVRLRDVAKIELGGENYDVIAKFNGQPASGLGIKLATGANAL 300
+ +EFGK+ L+VN DGS VRL+DVA++ELGGENY+VIA+ NG+PA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTATAIRAELKKMEPFFPPGMKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ 360
DTA AI+A+L +++PFFP GMK++YPYDTTPFV++SIHEVVKTL EAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
N RATLIPTIAVPVVLLGTFA+LAAFG+SINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 TEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480
E+ LPPKEAT KSM QIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATMLKPVAKGDHGEGKKGFFGWFNRLFDKSTHHYTDSVGNILRSTGR 540
SVLVALILTPALCAT+LKPV+ H E K GFFGWFN FD S +HYT+SVG IL STGR
Sbjct: 481 SVLVALILTPALCATLLKPVSAE-HHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 541 YLLLYLIIVVGMAYLFVRLPSSFLPDEDQGVFLTMVQLPAGATQERTQKVLDEVTDYYLN 600
YLL+Y +IV GM LF+RLPSSFLP+EDQGVFLTM+QLPAGATQERTQKVLD+VTDYYL
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 601 KEKANVESVFAVNGFGFAGRGQNTGIAFVSLKDWADRPGEKNKVEAITQRATAAFSQIKD 660
EKANVESVF VNGF F+G+ QN G+AFVSLK W +R G++N EA+ RA +I+D
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 661 AMVFAFNLPAIVELGTATGFDFELIDQAGLGHEKLTQARNQLFGEVAKYPDLLVGVRPNG 720
V FN+PAIVELGTATGFDFELIDQAGLGH+ LTQARNQL G A++P LV VRPNG
Sbjct: 660 GFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNG 719

Query: 721 LEDTPQFKIDIDQEKAQALGVSISDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYR 780
LEDT QFK+++DQEKAQALGVS+SDIN T+ A GG+YVNDFIDRGRVKK+YV ++AK+R
Sbjct: 720 LEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFR 779

Query: 781 MLPDDINDWYVRGSDGQMVPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEA 840
MLP+D++ YVR ++G+MVPFSAF++S W YGSPRLERYNGLPSMEI G+AAPG S+G+A
Sbjct: 780 MLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDA 839

Query: 841 MAMMEELASKLPSGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFS 900
MA+ME LASKLP+GIGYDWTGMSYQERLSGNQAPAL AIS +VVFLCLAALYESWSIP S
Sbjct: 840 MALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 901 VMLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGL 960
VMLVVPLG++G LLAAT NDVYF VGLLTTIGLSAKNAILIVEFAKDLM+KEGKG+
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGV 959

Query: 961 VEATLEAVRMRLRPILMTSLAFMLGVMPLVISSGAGSGAQNAVGTGVLGGMVTATVLAIF 1020
VEATL AVRMRLRPILMTSLAF+LGV+PL IS+GAGSGAQNAVG GV+GGMV+AT+LAIF
Sbjct: 960 VEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIF 1019

Query: 1021 FVPVFFVVVRRRF 1033
FVPVFFVV+RR F
Sbjct: 1020 FVPVFFVVIRRCF 1032


83SPA2310SPA2316N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2310020-0.643993nucleoside-specific channel-forming protein tsx
SPA23111160.535382hypothetical protein
SPA23121160.082723hypothetical protein
SPA23131160.142295deoR-family transcriptional regulator
SPA23141160.746391hypothetical protein
SPA23151171.091113protein-export membrane protein SecF
SPA23161170.817772protein-export membrane protein SecD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2310CHANNELTSX491e-180 Nucleoside-specific channel-forming protein Tsx signa...
		>CHANNELTSX#Nucleoside-specific channel-forming protein Tsx

signature.
Length = 294

Score = 491 bits (1266), Expect = e-180
Identities = 239/295 (81%), Positives = 254/295 (86%), Gaps = 9/295 (3%)

Query: 1 MKKTLLAVSAALALTSSFTANAAENDQPQYLSDWWHQSVNVVGSYHTRFSPKLNNDVYLE 60
MKKTLLA A +AL+++F A AAEND+PQYLSDWWHQSVNVVGSYHTRF P++ ND YLE
Sbjct: 1 MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60

Query: 61 YEAFAKKDWFDFYGYIDIPKTFDWGNGNDKGIWSDGSPLFMEIEPRFSIDKLTGADLSFG 120
YEAFAKKDWFDFYGYID P F GN KGIW+ GSPLFMEIEPRFSIDKLT DLSFG
Sbjct: 61 YEAFAKKDWFDFYGYIDAPVFFG-GNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFG 119

Query: 121 PFKEWYFANNYIYDMGDNKASRQSTWYMGLGTDIDTGLPMGLSLNVYAKYQWQNYGASNE 180
PFKEWYFANNYIYDMG N + QSTWYMGLGTDIDTGLPM LSLNVYAKYQWQNYGASNE
Sbjct: 120 PFKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNE 179

Query: 181 NEWDGYRFKVKYFVPITDLWGGKLNYIGFTNFDWGSDLGDDP--------NRTSNSIASS 232
NEWDGYRFKVKYFVP+TDLWGG L+YIGFTNFDWGSDLGDD RTSNSIASS
Sbjct: 180 NEWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASS 239

Query: 233 HILALNYDHWHYSVVARYFHNGGQWQNGAKLNWGDGDFSAKSTGWGGYLVVGYNF 287
HILALNY HWHYS+VARYFHNGGQW + AKLN+GDG FS +STGWGGY VVGYNF
Sbjct: 240 HILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2313ARGREPRESSOR334e-04 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 32.9 bits (75), Expect = 4e-04
Identities = 14/56 (25%), Positives = 24/56 (42%), Gaps = 5/56 (8%)

Query: 3 RRADRLFQIVQILRGRRLTT-----AALLAQRLAVSERTIYRDIRDLSLSGVPVEG 53
+ R +I +I+ + T L V++ T+ RDI++L L VP
Sbjct: 2 NKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKELHLVKVPTNN 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2315SECFTRNLCASE341e-120 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 341 bits (876), Expect = e-120
Identities = 102/306 (33%), Positives = 173/306 (56%), Gaps = 12/306 (3%)

Query: 1 MRWDFWAFGISGLLLIAAIVIMGVRGFNWGLDFTGGTVIEITLEKPAEMDVMREALQKAG 60
RW + FG + +++IA++++ V G N+G+DF GGT I ++ V R AL+
Sbjct: 17 FRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAALEPLE 76

Query: 61 YEEPQLQNFGS------SHDIMVRMPPTEGETGGQVLGSKVVTIINE------ATNQNAA 108
+ + H M+R+ E G + G++ ++N+ A +
Sbjct: 77 LGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAVDPALK 136

Query: 109 VKRIEFVGPSVGADLAQTGAMALLVALISILVYVGFRFEWRLAAGVVIALAHDVIITLGI 168
+ E VGP V +L T +LL A + I+ Y+ RFEW+ A G V+AL HDV++T+G+
Sbjct: 137 ITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTVGL 196

Query: 169 LSLFHIEIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRGTPYEIFNVSLTQTLHR 228
++ ++ DLT VA+L+++ GYS+ND++VV DR+REN K + ++ N+S+ +TL R
Sbjct: 197 FAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNETLSR 256

Query: 229 TLITSGTTLVVILMLYLFGGPVLEGFSLTMLIGVSIGTASSIYVASALALKLGMKREHML 288
T++T TTL+ ++ + ++GG V+ GF M+ GV GT SS+YVA + L +G+ R
Sbjct: 257 TVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIVLFIGLDRNKEK 316

Query: 289 QQKVEK 294
+ +K
Sbjct: 317 KDPSDK 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2316SECFTRNLCASE696e-15 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 69.5 bits (170), Expect = 6e-15
Identities = 35/165 (21%), Positives = 79/165 (47%), Gaps = 4/165 (2%)

Query: 422 IQIVEERTIGPTLGMQNIKQGLEACLAGLVVSILFMIF-FYKKFGLIATSALVANLVLIV 480
++I ++GP + + + + + LA VV + ++ F +F L A ALV +++L V
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 481 GIMSLLPGATLSMPGIAGIVLTLAVAVDANVLINERIKEEL--SNGRTVQQAINEGYAGA 538
G+ ++L + +A ++ +++ V++ +R++E L ++ +N
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 539 FSSIFDANITTLIKVIILYAVGTGAIKGFAITTGIGVATSMFTAI 583
S +TTL+ ++ + G I+GF GV T ++++
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSV 298


84SPA2325SPA2329N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA23251153.297026phosphate regulon sensor protein PhoR
SPA23261153.600524phosphate regulon transcriptional regulatory
SPA23272153.645260exonuclease SbcD
SPA23282143.219685exonuclease SbcC
SPA2329-1161.597850MFS family, arabinose polymer transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2325PF06580371e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 36.8 bits (85), Expect = 1e-04
Identities = 17/123 (13%), Positives = 38/123 (30%), Gaps = 28/123 (22%)

Query: 300 TFTFEVDDSLSVLGNEEQLRSAISNLVYNAVNH----TPAGTHITVSWRRVAHGAEFCIQ 355
F +++ ++ + + + LV N + H P G I + + ++
Sbjct: 241 QFENQINPAIM---DVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVE 297

Query: 356 DNGPGIAAEHIPRLTERFYRVDKARSRQTGGSGLGLAIVKHALNH---HESRLEIDSSPG 412
+ G +G GL V+ L E+++++ G
Sbjct: 298 NTGSLAL------------------KNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQG 339

Query: 413 KGT 415
K
Sbjct: 340 KVN 342


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2326HTHFIS823e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 81.8 bits (202), Expect = 3e-20
Identities = 27/131 (20%), Positives = 52/131 (39%), Gaps = 9/131 (6%)

Query: 6 LEQNGFQPVEAEDYDSAVNKLNEPWPDLILLDWMLPGGSGLQFIKHLKREAMTRDIPVVM 65
L + G+ + + + DL++ D ++P + + +K+ D+PV++
Sbjct: 23 LSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRIKKARP--DLPVLV 80

Query: 66 LTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDP 125
++A+ ++ E GA DY+ KPF EL+ I + E L D
Sbjct: 81 MSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA-------EPKRRPSKLEDDS 133

Query: 126 GSHRVMTGDSP 136
+ G S
Sbjct: 134 QDGMPLVGRSA 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2327FRAGILYSIN290.028 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 29.3 bits (65), Expect = 0.028
Identities = 14/70 (20%), Positives = 29/70 (41%), Gaps = 4/70 (5%)

Query: 149 KQQQLLHAIADYYQQQYQEACQLRGERKLPVIATGHLTTVGASKSDAVRDIYIGTLDAFP 208
K+ Q+++ IA++Y +++ + E++ T D + + I A
Sbjct: 135 KEAQMMNEIAEFYAAPFKKTRAIN-EKEAFECIYDSRTRSA--GKD-IVSVKINIDKAKK 190

Query: 209 AQHFPPADYI 218
+ P DYI
Sbjct: 191 ILNLPECDYI 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2328RTXTOXIND482e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.9 bits (114), Expect = 2e-07
Identities = 32/198 (16%), Positives = 71/198 (35%), Gaps = 13/198 (6%)

Query: 373 TQQSHDRAQLSQWQQQLLSDTRQRDALPPLTLDLTPQALAEARALHTRQRPLRHRLAALQ 432
TQ S +A+L Q + Q+LS + + + LP L L P + R L ++
Sbjct: 139 TQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSL------IK 192

Query: 433 GQILPKQKRQAQLQAAIARHHQEQTQYTQRLADKRLSYKTKAQELADVRTICEQ----EA 488
Q Q ++ Q + + + E+ R+ + + L D ++ + +
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKH 252

Query: 489 RIKDLESQRAHLQS--GQPCPLCGSTTHPAIAAYQALELSANQTRRDALEKEVKTLAEEG 546
+ + E++ + ++A + +L + + L+K +T
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNI- 311

Query: 547 AALRGQLDALTQQLQRDE 564
L +L ++ Q
Sbjct: 312 GLLTLELAKNEERQQASV 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2329TCRTETA509e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 49.8 bits (119), Expect = 9e-09
Identities = 68/356 (19%), Positives = 120/356 (33%), Gaps = 35/356 (9%)

Query: 5 IFSLALGTFGLGMAEFGIMGVLTELARDVGITIPAAGH---MISFYAFGVVLGAPVMALF 61
+ ++AL G+G+ IM VL L RD+ + H +++ YA APV+
Sbjct: 11 LSTVALDAVGIGL----IMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 62 SSRFSLKHILLFLVMLCVMGNAIFTFSSSYLMLAVGRLVSGFPHGAFFGVGAIVLSKIIR 121
S RF + +LL + + AI + +L +GR+V+G GA + I
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIAD--IT 124

Query: 122 PGKVTAAVAGMVSGMTVANLVGIPFGTYLSQEFSWRYTFLLIAVFNIAVLTAIFFWVPDI 181
G A G +S +V P L FS F A N F +P+
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPES 184

Query: 182 RDKAQGSLHEQ----------FHFLRSPAPWLI--FAATMFGNAGVFAWFSYIKPFMMYI 229
+ L + + A + F + G W +
Sbjct: 185 HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG------E 238

Query: 230 SGFSETSMTFIMMLVGLGM---VLGNLLSGKLSGRYTPLRIAVVTDLVIVLSLMALFFFS 286
F + T + L G+ + +++G ++ R R ++ ++ +
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLG---MIADGTGYILLA 295

Query: 287 GYKTASLTFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAF--NLGSAIG 340
+ F + + P +L E G G +A +L S +G
Sbjct: 296 FATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVG 351


85SPA2349SPA2356N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2349117-0.899467hypothetical protein
SPA23501170.318580flagellin structural protein
SPA23511173.439233delta-aminolevulinic acid dehydratase
SPA23521173.206926PrpE protein
SPA23531152.237202PrpD protein
SPA23541141.051058methylcitrate synthase
SPA2355-1151.431213carboxyvinyl-carboxyphosphonate
SPA2356-1140.973576propionate catabolism operon regulatory protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2349PF06291300.002 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 30.0 bits (67), Expect = 0.002
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 11/68 (16%)

Query: 28 VNDKEIICSPDESNTHTFVILEGVVSLVRGDKVLIGIVQAPFIFGLADGVAKKEAQYKLI 87
V +K +P E+ TH F VS + K V A I G A+ V K E Q +
Sbjct: 29 VGNKPTAVTPKETITHHFF-----VSGIGQKKT----VDAAKICGGAENVVKTETQQTFV 79

Query: 88 AESGCIGY 95
+G +G+
Sbjct: 80 --NGLLGF 85


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2350PRTACTNFAMLY1206e-30 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 120 bits (303), Expect = 6e-30
Identities = 100/436 (22%), Positives = 165/436 (37%), Gaps = 59/436 (13%)

Query: 597 TYSANGEADNSYTDNVVA---ATGNYKVRIDNATGAGSVADYKGNELIRVNDVNTDATFS 653
+ N AD +D +V A+G +++ + N+ GS L+ + + ATF+
Sbjct: 483 LFRMNVFADLGLSDKLVVMQDASGQHRLWVRNS---GSEPASANTLLLVQTPLGSAATFT 539

Query: 654 AAN---KADLGAYTYQAKQEGNTV------------------------------------ 674
AN K D+G Y Y+ GN
Sbjct: 540 LANKDGKVDIGTYRYRLAANGNGQWSLVGAKAPPAPKPAPQPGPQPPQPPQPQPEAPAPQ 599

Query: 675 VLEQMELTDYANMALSIP--SANTNIWNLEQDTVGTRLTNARHGLADNGGAWVSYFGGNF 732
EL+ AN A++ + +W E + + RL R D GGAW F
Sbjct: 600 PPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRL-NPDAGGAWGRGFAQRQ 658

Query: 733 NGDNGTIN-YDQDVNGIMVGVDTKVDGNNAKWIVGAAAGFAKGDLS---DRTGQVDQDSQ 788
DN +DQ V G +G D V +W +G AG+ +GD D G D
Sbjct: 659 QLDNRAGRRFDQKVAGFELGADHAVAVAGGRWHLGGLAGYTRGDRGFTGDGGGHTD---- 714

Query: 789 SAYIYSSARFANN--IFVDGNLSYSHFNNDLSANMSDGTYVDGNTSSDAWGFGLKLGYDL 846
S ++ A + + ++D L S ND SDG V G + G L+ G
Sbjct: 715 SVHVGGYATYIADSGFYLDATLRASRLENDFKVAGSDGYAVKGKYRTHGVGASLEAGRRF 774

Query: 847 KLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQAL 906
D ++ P ++ G Y+ +N ++V + S+ LG++ G + + +
Sbjct: 775 THADGWFLEPQAELAVFRAGGGAYRAANGLRVRDEGGSSVLGRLGLEVGKRIELAGGRQV 834

Query: 907 TPYFKLAYVYD-DSNNDADVNGDSIDNGVEGSAVRVGLGTQFSFTKNFSAYTDANYLGGG 965
PY K + + + D NG + + G+ +GLG + + S Y Y G
Sbjct: 835 QPYIKASVLQEFDGAGTVHTNGIAHRTELRGTRAELGLGMAAALGRGHSLYASYEYSKGP 894

Query: 966 DVDQDWSANVGVKYTW 981
+ W+ + G +Y+W
Sbjct: 895 KLAMPWTFHAGYRYSW 910


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2351BINARYTOXINB320.003 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 32.3 bits (73), Expect = 0.003
Identities = 19/69 (27%), Positives = 29/69 (42%)

Query: 254 DVLREIRERTELPLGAYQVSGEYAMIKFAAMAGAIDEEKVVLESLGSIKRAGADLIFSYF 313
+ E+ + +L L QV G A F +D E L I+ A +IF+
Sbjct: 466 NQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGK 525

Query: 314 ALDLAEKNI 322
L+L E+ I
Sbjct: 526 DLNLVERRI 534


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2356HTHFIS341e-114 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 341 bits (875), Expect = e-114
Identities = 119/376 (31%), Positives = 188/376 (50%), Gaps = 57/376 (15%)

Query: 192 ALDMTRLTRRQRVDYPSGKGLQTRYELGDIRGQSPQMEQLRQTITLYARSRAAVLIQGET 251
AL + + + + G+S M+++ + + ++ ++I GE+
Sbjct: 118 ALAEPKRRPSKL--------EDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGES 169

Query: 252 GTGKELAAQAIHQTFFHRQPHRQNKPSPPFVAVNCGAITESLLEAELFGYEEGAFTGSRR 311
GTGKEL A+A+H R+N P FVA+N AI L+E+ELFG+E+GAFTG++
Sbjct: 170 GTGKELVARALHD-----YGKRRNGP---FVAINMAAIPRDLIESELFGHEKGAFTGAQT 221

Query: 312 GGRAGLFEIAHGGTLFLDEIGEMPLPLQTRLLRVLEEKAVTRVGGHQPIPVEVRVISATH 371
G FE A GGTLFLDEIG+MP+ QTRLLRVL++ T VGG PI +VR+++AT+
Sbjct: 222 R-STGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATN 280

Query: 372 CDLDREIMQGRFRPDLFYRLSILRLTLPPLRERQADILPLAESFLKQSLAAMEIPFTESI 431
DL + I QG FR DL+YRL+++ L LPPLR+R DI L F++Q + +
Sbjct: 281 KDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQ-AEKEGLD----V 335

Query: 432 RHGLTQCQPLLLAWRWPGNIRELRNMMERLALFLS------------------------- 466
+ + L+ A WPGN+REL N++ RL
Sbjct: 336 KRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKA 395

Query: 467 -VDPAPTLDRQFMRQLLPELMVNTAELTPST---------VDAHTLQDVLARFKGDKSAA 516
Q + + + + + + P + ++ + L +G++ A
Sbjct: 396 AARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKA 455

Query: 517 ARYLGISRTTLWRRLK 532
A LG++R TL ++++
Sbjct: 456 ADLLGLNRNTLRKKIR 471


86SPA2543SPA2551N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA25432153.961024DNA repair protein
SPA25443154.242373small protein A
SPA25452144.265277hypothetical protein
SPA25462144.074417hypothetical protein
SPA25472143.901590SsrA (tmRNA)-binding protein
SPA25482153.991785hypothetical protein
SPA2549-2111.247792type I secretion protein
SPA2550-1130.426645type I secretion protein, ATP-binding protein
SPA2551323-1.722722type I secretion protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2543RTXTOXIND310.009 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.009
Identities = 31/198 (15%), Positives = 64/198 (32%), Gaps = 36/198 (18%)

Query: 177 QQQSQERAARAELLQYQLKELNDFNPQAGEFEQIDEEYKRLANSGQLLTTSQNALALLAD 236
+ QS AR E +YQ+ + + E + DE Y + + ++L + +
Sbjct: 138 KTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFS- 196

Query: 237 GEDVNLQSQLYSAKQLVSELVGMDSKLSGILDMLEEATIQLTEASDELRHYCERLDLDPN 296
Q+Q Y Q L ++ +L A I E +
Sbjct: 197 ----TWQNQKY---QKELNLDKKRAERLTVL-----ARINRYENLSRV------------ 232

Query: 297 RLFELEQRIAKQISLARKHHVSPEALPQLYQSLLEEQQQLDDQADSLETLTLAVNKHHQQ 356
+ R+ SL K ++ ++LE++ + + + L + + +
Sbjct: 233 ----EKSRLDDFSSLLHKQAIA-------KHAVLEQENKYVEAVNELRVYKSQLEQIESE 281

Query: 357 ALETAQALHQQRQFYAQE 374
L + Q + E
Sbjct: 282 ILSAKEEYQLVTQLFKNE 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2546FLGMOTORFLIM280.018 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 27.6 bits (61), Expect = 0.018
Identities = 16/78 (20%), Positives = 30/78 (38%), Gaps = 8/78 (10%)

Query: 36 GSRVLESSPAQMTAAVDVSKAGISKTFTTRNQLTRNQSILMHLVDGPFKKLIGGWK---- 91
G+ VLE P+ + +D G + + LT I +++G +++ +
Sbjct: 113 GNAVLEVDPSITFSIIDRLFGGTGQAAKVQRDLT---DIENSVMEGVIVRILANVRESWT 169

Query: 92 -FTPLSPEACRIEFQLDF 108
L P +IE F
Sbjct: 170 QVIDLRPRLGQIETNPQF 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2548INTIMIN463e-06 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 46.2 bits (109), Expect = 3e-06
Identities = 63/315 (20%), Positives = 107/315 (33%), Gaps = 38/315 (12%)

Query: 2724 TPAQTNGQPLLAFAQDKAGNTGIAAGFTAPDTRVPEAPIITNVVDDVGIYTGAIANGQ-- 2781
+N + A A D+ GN+ T + V D T A A+G
Sbjct: 518 VQGGSNVYKVTARAYDRNGNSSNNVLLTITVLSNGQVVDQVGVTDFTADKTSAKADGTEA 577

Query: 2782 VTNDAQPTLNGTAQAGATVS--IYNNGALLGTTTANASGNWSFTPTGNLTEGSHAFT-AT 2838
+T A NG AQA VS I + A+L +AN +G+ T T L +
Sbjct: 578 ITYTATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVT--LKSDKPGQVVVS 635

Query: 2839 ATNANGTGSVSSAATVIVDTLAPGTPSGTLSADGGSLSGLAEANSTVTVTLT-------- 2890
A A T ++++ A + VD + AD + +A +T T+
Sbjct: 636 AKTAEMTSALNANAVIFVDQTK--ASITEIKAD--KTTAVANGQDAITYTVKVMKGDKPV 691

Query: 2891 -----------GGVTLTT-TAGSNGAWSLTLPTKQIEGQLINVTATDAAGN-ASGTLGIT 2937
G ++ +T +NG +TL + L++ +D A + + +
Sbjct: 692 SNQEVTFTTTLGKLSNSTEKTDTNGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFF 751

Query: 2938 APVLPLAARDNITSLDLTSTAVTSTQNYSDYGLLLVGALGNVASVLGN------DTTQVE 2991
+ I + T Y L G G N D + +
Sbjct: 752 TTLTIDDGNIEIVGTGVKGKLPTVWLQYGQVNLKASGGNGKYTWRSANPAIASVDASSGQ 811

Query: 2992 FTIAEGGTGDVTIDA 3006
T+ E GT +++ +
Sbjct: 812 VTLKEKGTTTISVIS 826



Score = 41.6 bits (97), Expect = 7e-05
Identities = 64/272 (23%), Positives = 91/272 (33%), Gaps = 22/272 (8%)

Query: 1508 TLPVTSALPDGVYTLTAIAADAAGNSSGVSNSFTFTVDTVPLQPPVVN--EILDDVAPVT 1565
LP VY +TA A D GNS SN+ T+ TV VV+ + D A T
Sbjct: 513 ILPAYVQGGSNVYKVTARAYDRNGNS---SNNVLLTI-TVLSNGQVVDQVGVTDFTADKT 568

Query: 1566 GPLTDG--AFTNDRTLTINGSGENGSTVTIYDNGVAIGTALVTDGVWTFN-----TSELS 1618
DG A T T+ NG + V+ + GTA+++ N T L
Sbjct: 569 SAKADGTEAITYTATVKKNGVAQANVPVSF---NIVSGTAVLSANSANTNGSGKATVTLK 625

Query: 1619 EASHALTFSATDDAGNTTAQTQPITITVDITAPPAPTIQTVADDGTRVAGLADPYA-TVE 1677
+ A T+A I VD T I+ AD T VA D TV+
Sbjct: 626 SDKPGQVVVSAKTAEMTSALNANAVIFVDQTKASITEIK--ADKTTAVANGQDAITYTVK 683

Query: 1678 IHHADGTLVGSAVANGTGEFVVTLSPAQTDGGTLTAIAIDRAGNNGPATNFPASDSGLPA 1737
+ D + V T ++ S +TD + + + G +
Sbjct: 684 VMKGDKPVSNQEVTFTTTLGKLSNSTEKTDTNGYAKVTLT-STTPGKSLVSARVSDVAVD 742

Query: 1738 VPAITAIEDDVGSIQGNIAA--GGATDDTMPT 1767
V A +I G +PT
Sbjct: 743 VKAPEVEFFTTLTIDDGNIEIVGTGVKGKLPT 774



Score = 37.4 bits (86), Expect = 0.001
Identities = 75/370 (20%), Positives = 137/370 (37%), Gaps = 45/370 (12%)

Query: 2197 IYNGSALVGTA-QVQANGSWSFT-------PSTSLGAGVWNLTATATDAAGNTSAASEIR 2248
+++ SAL Q+Q +GS S G+ V+ +TA A D GN+S + +
Sbjct: 486 VWDDSALRSQGGQIQHSGSQSAQDYQAILPAYVQGGSNVYKVTARAYDRNGNSS-NNVLL 544

Query: 2249 SFTIDTTAPAAPVIDTVYDGTGPITGNLSSGQ--ITDEARPVISGTREAN--TAIRLYDN 2304
+ T+ + V D T T + G IT A +G +AN + +
Sbjct: 545 TITV-LSNGQVVDQVGVTDFTADKTSAKADGTEAITYTATVKKNGVAQANVPVSFNIVSG 603

Query: 2305 GTLLAEIPADNSSSWRYTPDASLATGNHVITVIAVDAAGNASPV-SDSVNFVVDTTPPLT 2363
+L+ A+ + S + T +L + V++ A S + +++V FV T +T
Sbjct: 604 TAVLSANSANTNGSGKAT--VTLKSDKPGQVVVSAKTAEMTSALNANAVIFVDQTKASIT 661

Query: 2364 PVITSVSDDQAPGLGTIANGQN--TNDPTPTFSGTAEAGATITLYENGTVIGTTTAQ--P 2419
+ A +ANGQ+ T + +T + +T +
Sbjct: 662 EI--KADKTTA-----VANGQDAITYTVKVMKGDKPVSNQEVTFTTTLGKLSNSTEKTDT 714

Query: 2420 DGAWSVSTSTLASGTHVITAVATDAAGNSSPNSTAFTLTVDTTAPQTPILTSVVDDVAGG 2479
+G V+ ++ G +++A +D A + F I ++ V G
Sbjct: 715 NGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFF-------TTLTIDDGNIEIVGTG 767

Query: 2480 VTGNLANGQITNDNRPTLNGTAEAGSV-VTIYDGNTLLGVTSANAGGAWSFTPTTGLNDG 2538
V G L + +N A G+ T N + A++G T G
Sbjct: 768 VKGKLPTVWL---QYGQVNLKASGGNGKYTWRSANPAIASVDASSG------QVTLKEKG 818

Query: 2539 TRILTVTATD 2548
T ++V ++D
Sbjct: 819 TTTISVISSD 828


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2549RTXTOXIND364e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 35.6 bits (82), Expect = 4e-04
Identities = 32/224 (14%), Positives = 63/224 (28%), Gaps = 32/224 (14%)

Query: 209 DVVQTEARIESARSQLAQYQANLDSAKASLMSWLGWNSLNGINNDFPAKLARSCETATPD 268
+ EA +S L Q + + S D P S E
Sbjct: 128 TALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRL 187

Query: 269 DRLVPAVLAAW-AQANVARANLDYASAQ---MTPTISLEPSVQHYLNDKYPSHEVLDKTQ 324
L+ + W Q NLD A+ + I+ ++ + L Q
Sbjct: 188 TSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQ 247

Query: 325 YSTWVKVEMPLYQGGGLTARRNAASHAVDAAQSTIQRTRLDVRQKLMEARSQAMSLASAL 384
V + ++ +L +SQ + S
Sbjct: 248 AIAKHAVL-------------------------EQENKYVEAVNELRVYKSQLEQIES-- 280

Query: 385 QILRRQQQLSERTRELYQQQYLDLGSRPLLDVLNAEQEVYQARF 428
+IL +++ T +L++ + LD + ++ E+ +
Sbjct: 281 EILSAKEEYQLVT-QLFKNEILDKLRQTTDNIGLLTLELAKNEE 323


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2551RTXTOXIND2433e-78 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 243 bits (621), Expect = 3e-78
Identities = 95/432 (21%), Positives = 176/432 (40%), Gaps = 56/432 (12%)

Query: 9 ERAFSGAGRIVLICSLLFLILGI-WAWFGRLDEVSTGNGKVIPSSREQVLQSLDGGILAQ 67
E S R+V + FL++ + G+++ V+T NGK+ S R + ++ ++ I+ +
Sbjct: 50 ETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKE 109

Query: 68 LTVREGDRVQANQIVARLDPTRLASNVGESAAKYRASLASSARLTAEVSDLPL------- 120
+ V+EG+ V+ ++ +L ++ ++ + + R + L
Sbjct: 110 IIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELK 169

Query: 121 --AFPAELNGWPDLIAAETRLYKSR-----------RAQLADTEAELRDALASVNK---- 163
P N + + T L K + L AE LA +N+
Sbjct: 170 LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENL 229

Query: 164 ------ELTITQRLEKSGAASHVEVLRLQRQKSDLG---------------------LKI 196
L L A + VL + + + +
Sbjct: 230 SRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEY 289

Query: 197 TDLRSQYYVQAREALSKANAEVDMLSAILKGREDSVTRLTVRSPVRGIVKNIQVTTIGGV 256
+ + + + L + + +L+ L E+ +R+PV V+ ++V T GGV
Sbjct: 290 QLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGV 349

Query: 257 IPPNGEMMEIVPVDDRLLIETRLSPRDIAFIHPGQRALVKITAYDYAIYGGLDGVVETIS 316
+ +M IVP DD L + + +DI FI+ GQ A++K+ A+ Y YG L G V+ I+
Sbjct: 350 VTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNIN 409

Query: 317 PDTIQDKVKPEIFYYRVFIRTHQDYLQNKSGRRFSIVPGMIATVDIKTGEKTIVDYLIKP 376
D I+D+ + V I ++ L + + GM T +IKTG ++++ YL+ P
Sbjct: 410 LDAIEDQRLGL--VFNVIISIEENCLSTG-NKNIPLSSGMAVTAEIKTGMRSVISYLLSP 466

Query: 377 F-NRAKEALRER 387
E+LRER
Sbjct: 467 LEESVTESLRER 478


87SPA2669SPA2675N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2669-2142.041573glycine betaine-binding periplasmic protein
SPA2670-2101.490633transmembrane transport protein
SPA2671-2100.486366transcriptional regulator
SPA2672-2101.223895multidrug resistance protein A
SPA2673-2131.072933multidrug resistance protein B
SPA2675-1130.436615autoinducer-2 production protein LuxS
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2669PF06057290.014 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 29.4 bits (66), Expect = 0.014
Identities = 8/55 (14%), Positives = 17/55 (30%)

Query: 277 FAIMKLPLADINAQNAMMHAGKSSEADVQGHVDGWINAHQQQFDGWVKEALAAQK 331
F + ++P + S +D + HV + + Q + Q
Sbjct: 133 FVLNEMPARYRKNVLGAVLLSPSQSSDFEIHVSEMVTSDNQSARYLTLPEVNKQT 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2670TCRTETB461e-07 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 46.4 bits (110), Expect = 1e-07
Identities = 31/165 (18%), Positives = 66/165 (40%), Gaps = 2/165 (1%)

Query: 34 LDTIAHHFSLSASSAGFIVTAAQLGYAAGLLFLVPLGDMFE-RRTLIVSMTLLAAGGMLI 92
L IA+ F+ +S ++ TA L ++ G L D +R L+ + + G ++
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 93 TASSQSLSMMILGTALTGLFSVVAQILVPLA-ATLATPATRGKVVGTIMSGLLLGILLAR 151
S++I+ + G + LV + A RGK G I S + +G +
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 152 TVAGLLANLGGWRTVFWVASALMALMAVALWRGLPKLKSDTHLNY 196
+ G++A+ W + + + + + +++ H +
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2672RTXTOXIND741e-16 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 74.5 bits (183), Expect = 1e-16
Identities = 62/418 (14%), Positives = 125/418 (29%), Gaps = 97/418 (23%)

Query: 19 KRKTALLLLTLLFVIIAVAYGIYWFLVLRHIEETDDA----YVAGNQVQIMAQVSGSVTK 74
+ L F++ + VL +E A +G +I + V +
Sbjct: 51 TPVSRRPRLVAYFIMGFLVIAFILS-VLGQVEIVATANGKLTHSGRSKEIKPIENSIVKE 109

Query: 75 VWADNTDFVKEGDVLVTLDQT--------------------------------------- 95
+ + V++GDVL+ L
Sbjct: 110 IIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELK 169

Query: 96 -------------DAKQAFERAKTALASSVRQTHQLMINSKQ-------LQANIDVQKTA 135
+ + K ++ Q +Q +N + + A I+ +
Sbjct: 170 LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENL 229

Query: 136 LAQAQSDLNRRVPLGNANLIGREELQHARDAVASAQAQLDVAIQQYNANQAMILNSNLED 195
+S L+ L + I + + + A +L V Q ++ IL++ E
Sbjct: 230 SRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEY 289

Query: 196 QPAVQQAATEVRN------------------AWLALERTRIVSPMTGYVSRRAVQ-PGAQ 236
Q Q E+ + + + I +P++ V + V G
Sbjct: 290 QLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGV 349

Query: 237 ISPTTPLMAVVPATD-LWVDANFKETQLANMRIGQPVTIITDIYGDDVKY---TGKVVGL 292
++ LM +VP D L V A + + + +GQ I + + +Y GKV +
Sbjct: 350 VTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAF-PYTRYGYLVGKVKNI 408

Query: 293 DMGTGSAFSLLPAQNATGNWIKVVQRLPVRVELDARQLEQHPLRIGLSTLVTVDTANR 350
+ ++ G V+ + + PL G++ + T R
Sbjct: 409 -----NLDAIE--DQRLGLVFNVIISIEENCLSTGNK--NIPLSSGMAVTAEIKTGMR 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2673TCRTETB1298e-35 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 129 bits (326), Expect = 8e-35
Identities = 94/405 (23%), Positives = 164/405 (40%), Gaps = 23/405 (5%)

Query: 17 IALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRF 76
I L + +F VL+ + NV++P IA + + WV T+F + +I + G L+ +
Sbjct: 17 IWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQL 76

Query: 77 GEVKLFMWSTVAFAAASWACGVS-SGLNMLIFFRVVQGVVAGPLIPLSQSLLLNNYPPAK 135
G +L ++ + S V S ++LI R +QG A L ++ P
Sbjct: 77 GIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKEN 136

Query: 136 RSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGIAVVLMTLHTLRGRETH 195
R A L V + GP +GG I+ HW ++ I + I I V + L+
Sbjct: 137 RGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM-ITIITVPFLMKLLKKEVRI 195

Query: 196 TERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVVAVIAISFLIVWELTD 255
D G+ L+ +GI + ML F++ I +V+V++ +
Sbjct: 196 KG--HFDIKGIILMSVGI--VFFML--------FTTSYSISFLIVSVLSFLIFVKHIRKV 243

Query: 256 DHPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGI 315
P VD L K+ F IG LC + + G + ++P ++++V+ + G G
Sbjct: 244 TDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGT 303

Query: 316 IPVILS-PIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGF- 373
+ VI+ I G + ++ +V F ++ S + I F
Sbjct: 304 MSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFL-----LETTSWFMTIIIVFV 358

Query: 374 --AVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSI 416
++ ++TI S L + A SL NFT L+ G +I
Sbjct: 359 LGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAI 403


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2675LUXSPROTEIN287e-103 Bacterial autoinducer-2 (AI-2) production protein Lu...
		>LUXSPROTEIN#Bacterial autoinducer-2 (AI-2) production protein LuxS

signature.
Length = 171

Score = 287 bits (736), Expect = e-103
Identities = 130/170 (76%), Positives = 145/170 (85%)

Query: 2 PLLDSFAVDHTRMQAPAVRVAKTMNTPHGDAITVFDLRFCIPNKEVMPEKGIHTLEHLFA 61
PLLDSF VDHTRM APAVRVAKTM TP GD ITVFDLRF PNK+++ EKGIHTLEHL+A
Sbjct: 1 PLLDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTAPNKDILSEKGIHTLEHLYA 60

Query: 62 GFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVADAWKAAMADVLKVQDQNQIP 121
GFMR+HLNG+ VEIIDISPMGCRTGFYMSLIGTP EQ+VADAW AAM DVLKV++QN+IP
Sbjct: 61 GFMRNHLNGDSVEIIDISPMGCRTGFYMSLIGTPSEQQVADAWIAAMEDVLKVENQNKIP 120

Query: 122 ELNVYQCGTYQMHSLSEAQDIARHILERDVRVNSNKELALPKEKLQELHI 171
ELN YQCGT MHSL EA+ IA++ILE V VN N ELALP+ L+EL I
Sbjct: 121 ELNEYQCGTAAMHSLDEAKQIAKNILEVGVAVNKNDELALPESMLRELRI 170


88SPA2736SPA2756N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA2736232-8.349909stpA-like protein
SPA2737028-7.287448chaperone (associated with virulence)
SPA2738125-5.217684hypothetical protein
SPA2739125-5.263052acyl carrier protein
SPA2740123-5.646466pathogenicity island 1 effector protein
SPA2741121-5.457526pathogenicity island 1 effector protein
SPA2742122-5.332648pathogenicity island 1 effector protein
SPA2743122-6.050004pathogenicity island 1 effector protein
SPA2744-127-7.033173hypothetical protein
SPA2745-127-6.157893secretory protein (associated with virulence)
SPA2746-127-5.499219secretory protein (associated with virulence)
SPA2747-223-3.938679secretory protein (associated with virulence)
SPA2748-224-4.247423secretory protein (associated with virulence)
SPA2749-224-4.594316surface presentation of antigens protein
SPA2750-123-5.714108antigen presentation protein SpaN
SPA2751-224-6.199650virulence-associated secretory protein
SPA2752-224-6.056000virulence-associated secretory apparatus ATP
SPA2753-127-7.845117virulence-associated secretory protein
SPA2754-125-7.472499secretory protein
SPA2755-127-7.222594cell invasion protein
SPA2756128-6.932021secretory protein (associated with virulence)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2736BACYPHPHTASE3031e-99 Salmonella/Yersinia modular tyrosine phosphatase si...
		>BACYPHPHTASE#Salmonella/Yersinia modular tyrosine phosphatase

signature.
Length = 468

Score = 303 bits (777), Expect = 1e-99
Identities = 68/212 (32%), Positives = 102/212 (48%), Gaps = 17/212 (8%)

Query: 340 GKPVALAGSYPKNTPDALEAHMKMLLEKECSCLAVLTSEDQMQAKQ--LPAYFRGSYTFG 397
G +A YP LE+H +ML E LAVL S ++ ++ +P YFR S T+G
Sbjct: 252 GNTRTIACQYP--LQSQLESHFRMLAENRTPVLAVLASSSEIANQRFGMPDYFRQSGTYG 309

Query: 398 EVHTNSQKVSSASQGGAI--DQYNMQL-SCGEKRYTIPVLHVKNWPDHQPLPS--TDQLE 452
+ S+ G I D Y + + G+K ++PV+HV NWPD + S T L
Sbjct: 310 SITVESKMTQQVGLGDGIMADMYTLTIREAGQKTISVPVVHVGNWPDQTAVSSEVTKALA 369

Query: 453 YLADRVKNSNQNGAPGRSSS-----DKHLPMIHCLGGVGRTGTMAAALVLKDNPHSNL-- 505
L D+ + +N + SS K P+IHC GVGRT + A+ + D+ +S L
Sbjct: 370 SLVDQTAETKRNMYESKGSSAVGDDSKLRPVIHCRAGVGRTAQLIGAMCMNDSRNSQLSV 429

Query: 506 EQVRADFRNSRNNRMLEDASQF-VQLKAMQAQ 536
E + + R RN M++ Q V +K + Q
Sbjct: 430 EDMVSQMRVQRNGIMVQKDEQLDVLIKLAEGQ 461


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2737PF05932345e-05 Tir chaperone protein (CesT)
		>PF05932#Tir chaperone protein (CesT)

Length = 127

Score = 33.6 bits (77), Expect = 5e-05
Identities = 16/111 (14%), Positives = 40/111 (36%), Gaps = 7/111 (6%)

Query: 4 PLTFDDNNQCLLLLDSDIFTSIEAK--DDIWLLNGMIIPLSPVCGDSIWRQIMVINGELA 61
PL FDD+ C +++D+ ++ + LL G++ P D + ++
Sbjct: 21 PLVFDDHGTCNMIIDNTFALTLSCDYARERLLLIGLLEPH----KDIPQQCLLAGALNPL 76

Query: 62 ANNEGTLAYIDAAETLLFIHAI-TDLTNIYHIISQLESFVNKQEALKNILQ 111
N L + + +I + ++ + ++ + + Q
Sbjct: 77 LNAGPGLGLDEKSGLYHAYQSIPREKLSVPTLKREMAGLLEWMRGWREASQ 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2742BACINVASINC5140.0 Salmonella/Shigella invasin protein C signature.
		>BACINVASINC#Salmonella/Shigella invasin protein C signature.

Length = 409

Score = 514 bits (1324), Expect = 0.0
Identities = 407/409 (99%), Positives = 407/409 (99%)

Query: 1 MLISNVGINPAAYLNNHSVENSSQTASQSVSAKDILNSIGISSSKVSDLGLSPTLSAPAP 60
MLISNVGINPAAYLNNHSVENSSQTASQSVSAKDILNSIGISSSKVSDLGLSPTLSAPAP
Sbjct: 1 MLISNVGINPAAYLNNHSVENSSQTASQSVSAKDILNSIGISSSKVSDLGLSPTLSAPAP 60

Query: 61 GVLTQTPGTITSFLKASIQNTDMNQDLNALANNVTTKANEVVQTQLREQQAEVGKFFDIS 120
GVLTQTPGTITSFLKASIQNTDMNQDLNALANNVTTKANEVVQTQLREQQAEVGKFFDIS
Sbjct: 61 GVLTQTPGTITSFLKASIQNTDMNQDLNALANNVTTKANEVVQTQLREQQAEVGKFFDIS 120

Query: 121 GMSSSAVALLAAANTLMLTLNQADSKLSGKLSLVSFDAAKTTASSMMREGMNALSGSISQ 180
GMSSSAVALLAAANTLMLTLNQADSKLSGKLSLVSFDAAKTTASSMMREGMNALSGSISQ
Sbjct: 121 GMSSSAVALLAAANTLMLTLNQADSKLSGKLSLVSFDAAKTTASSMMREGMNALSGSISQ 180

Query: 181 SALQLGITGVGAKLEYKGLQNERGALKHNAAKIDKLTTESHSIKNVLNGQNSVKLGAEGV 240
SALQLGITGVGAKLEYKGLQNERGALKHNAAKIDKLTTESHSIKNVLNGQNSVKLGAEGV
Sbjct: 181 SALQLGITGVGAKLEYKGLQNERGALKHNAAKIDKLTTESHSIKNVLNGQNSVKLGAEGV 240

Query: 241 DSLKSLNMKKTGTDATKNLNDATLKSNAGTSATESLGIKNSNKQISPEHQAILSKRLESV 300
DSLKSLNMKKTGTDATKNLNDATLKSNAGTSATESLGIKNSNKQISPEHQAILSKRLESV
Sbjct: 241 DSLKSLNMKKTGTDATKNLNDATLKSNAGTSATESLGIKNSNKQISPEHQAILSKRLESV 300

Query: 301 ESDIRLEQNTMDMTRIDARKMQMTGDLIMKNLVTVGGIAGASGQYAATQERSEQQISQVN 360
ESDIRLEQNTMDMTRIDARKMQMTGDLIMKN VTVGGIAGAS QYAATQERSEQQISQVN
Sbjct: 301 ESDIRLEQNTMDMTRIDARKMQMTGDLIMKNSVTVGGIAGASRQYAATQERSEQQISQVN 360

Query: 361 NRVASTASDEARESSRKSTSLIQEMLKTMESINQSKASALAAIAGNIRA 409
NRVASTASDEARESSRKSTSLIQEMLKTMESINQSKASALAAIAGNIRA
Sbjct: 361 NRVASTASDEARESSRKSTSLIQEMLKTMESINQSKASALAAIAGNIRA 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2743BACINVASINB8350.0 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 835 bits (2158), Expect = 0.0
Identities = 590/593 (99%), Positives = 590/593 (99%)

Query: 1 MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGE 60
MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGE
Sbjct: 1 MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGE 60

Query: 61 SAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKE 120
SAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKE
Sbjct: 61 SAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKE 120

Query: 121 MGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAAAKKLTQAQNKLQSLDPADPG 180
MGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAA KKLTQAQNKLQSLDPADPG
Sbjct: 121 MGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAATKKLTQAQNKLQSLDPADPG 180

Query: 181 YAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQN 240
YAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQN
Sbjct: 181 YAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQN 240

Query: 241 QVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEF 300
QVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEF
Sbjct: 241 QVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEF 300

Query: 301 QEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAA 360
QEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAA
Sbjct: 301 QEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAA 360

Query: 361 TGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMV 420
TGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMV
Sbjct: 361 TGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMV 420

Query: 421 AVIAVVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLG 480
AVI VVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLG
Sbjct: 421 AVIVVVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLG 480

Query: 481 NVGSKMGLQTNALSKELVGNTLNKVALSMEVTNTAAQSAGGVAEGVFIKNASEALADFML 540
NVGSKMGLQTNALSKELVGNTLNKVAL MEVTNTAAQSAGGVAEGVFIKNASEALADFML
Sbjct: 481 NVGSKMGLQTNALSKELVGNTLNKVALGMEVTNTAAQSAGGVAEGVFIKNASEALADFML 540

Query: 541 ARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA 593
ARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA
Sbjct: 541 ARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA 593


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2744SYCDCHAPRONE1282e-40 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 128 bits (322), Expect = 2e-40
Identities = 39/160 (24%), Positives = 72/160 (45%), Gaps = 4/160 (2%)

Query: 4 QNNVSEERVAEMIWDAVSEGATLKDVHGIPQDMMDGLYAHAYEFYNQGRLDEAETFFRFL 63
Q + + + G T+ ++ I D ++ LY+ A+ Y G+ ++A F+ L
Sbjct: 3 QETTDTQEYQLAMESFLKGGGTIAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQAL 62

Query: 64 CIYDFYNPDYTMGLAAVCQLKKQFQKACDLYAVAFTLLKNDYRPVFFTGQCQLLMRKAAK 123
C+ D Y+ + +GL A Q Q+ A Y+ + + R F +C L + A+
Sbjct: 63 CVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAE 122

Query: 124 ARQCF----ELVNERTEDESLRAKALVYLEALKTAETEQH 159
A EL+ ++TE + L + LEA+K + +H
Sbjct: 123 AESGLFLAQELIADKTEFKELSTRVSSMLEAIKLKKEMEH 162


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2745TYPE3IMSPROT340e-118 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 340 bits (874), Expect = e-118
Identities = 119/360 (33%), Positives = 204/360 (56%), Gaps = 19/360 (5%)

Query: 1 MSSNKTEKPTKKRLEDSAKKGQSFKSKDLIIACLTLGGIAYLVSYGSFN-EFMGIIKIII 59
MS KTE+PT K++ D+ KKGQ KSK+++ L + A L+ + E + +I
Sbjct: 1 MSGEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIP 60

Query: 60 ADNFDQSMADYSLAVFGIGLKYLIPFMLLCL---VCSALPAL----LQAGFVLATEALKP 112
+QS +S A+ + L+ F LC +AL A+ +Q GF+++ EA+KP
Sbjct: 61 ---AEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKP 117

Query: 113 NLSALNPVEGAKKLFSMRTVKDTVKTLLYLSSFVVAAIICWKKYKVEIFSQLNGNVVDIA 172
++ +NP+EGAK++FS++++ + +K++L + V+ +I+ W K + + L I
Sbjct: 118 DIKKINPIEGAKRIFSIKSLVEFLKSILKV---VLLSILIWIIIKGNLVTLLQLPTCGIE 174

Query: 173 VIWRELLLALVLTCLACA---LIVLLLDAIAEYFLTMKDMKMDKEEVKREMKEQEGNPEV 229
I L L + C +++ + D EY+ +K++KM K+E+KRE KE EG+PE+
Sbjct: 175 CITPLLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEI 234

Query: 230 KSKRREVHMEILSEQVKSDIENSRLIVANPTHITIGIYFKPELMPIPMISVYETNQRALA 289
KSKRR+ H EI S ++ +++ S ++VANPTHI IGI +K P+P+++ T+ +
Sbjct: 235 KSKRRQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQT 294

Query: 290 VRAYAEKVGVPVIVDIKLARSLFKTHRRYDLVSLEEIDEVLRLLVWLE--EVENAGKDVI 347
VR AE+ GVP++ I LAR+L+ + E+I+ +L WLE +E +++
Sbjct: 295 VRKIAEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNIEKQHSEML 354


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2746TYPE3IMRPROT1882e-61 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 188 bits (479), Expect = 2e-61
Identities = 50/248 (20%), Positives = 107/248 (43%), Gaps = 4/248 (1%)

Query: 1 MLYALYFEIHHLVASAALGFARVAPIFFFLPFLNSGVLSGAPRNAIIILVALGVWPHALN 60
ML + + RV + P L+ + + + +++ + P
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 EAPPFLSVAMIPLVLQEAAVGVMLGCLLSWPFWVMHALGCIIDNQRGATLSSSIDPANGI 120
P S + L +Q+ +G+ LG + + F + G II Q G + ++ +DPA+ +
Sbjct: 61 NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHL 120

Query: 121 DTSEMANFLNMFAAVVYLQNGGLVTMVDVLNKSYQLCDPMNEC--TPSLPPLLTFINQVA 178
+ +A ++M A +++L G + ++ +L ++ E + + L + +
Sbjct: 121 NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIF 180

Query: 179 QNALVLASPVVLVLLLSEVFLGLLSRFAPQMNAFAISLTVKSGIAVLIMLLYFS--PVLP 236
N L+LA P++ +LL + LGLL+R APQ++ F I + + + +M
Sbjct: 181 LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC 240

Query: 237 DNVLRLSF 244
+++ F
Sbjct: 241 EHLFSEIF 248


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2747TYPE3IMQPROT894e-27 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 88.7 bits (220), Expect = 4e-27
Identities = 86/86 (100%), Positives = 86/86 (100%)

Query: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL 60
MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL
Sbjct: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL 60

Query: 61 FLLSGWYGEVLLSYGRQVIFLALAKG 86
FLLSGWYGEVLLSYGRQVIFLALAKG
Sbjct: 61 FLLSGWYGEVLLSYGRQVIFLALAKG 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2748TYPE3IMPPROT303e-107 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 303 bits (777), Expect = e-107
Identities = 223/224 (99%), Positives = 223/224 (99%)

Query: 1 MGNDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLS 60
MGNDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLS
Sbjct: 1 MGNDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLS 60

Query: 61 MFVMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLSKYSDRELVQFFENAQL 120
MFVMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYL KYSDRELVQFFENAQL
Sbjct: 61 MFVMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLIKYSDRELVQFFENAQL 120

Query: 121 KRQYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVL 180
KRQYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVL
Sbjct: 121 KRQYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVL 180

Query: 181 LALGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQYMDIAT 224
LALGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQYMDIAT
Sbjct: 181 LALGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQYMDIAT 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2749TYPE3OMOPROT5370.0 Type III secretion system outer membrane O protein ...
		>TYPE3OMOPROT#Type III secretion system outer membrane O protein

family signature.
Length = 303

Score = 537 bits (1384), Expect = 0.0
Identities = 302/303 (99%), Positives = 302/303 (99%)

Query: 1 MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL 60
MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL
Sbjct: 1 MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL 60

Query: 61 EHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL 120
EHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL
Sbjct: 61 EHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL 120

Query: 121 HIMSDRGGLWFEHLPELPAVAGGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS 180
HIMSDRGGLWFEHLPELPAV GGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS
Sbjct: 121 HIMSDRGGLWFEHLPELPAVGGGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS 180

Query: 181 RAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR 240
RAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR
Sbjct: 181 RAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR 240

Query: 241 KNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG 300
KNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG
Sbjct: 241 KNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG 300

Query: 301 NGE 303
NGE
Sbjct: 301 NGE 303


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2750SSPANPROTEIN6010.0 Salmonella invasion protein InvJ signature.
		>SSPANPROTEIN#Salmonella invasion protein InvJ signature.

Length = 336

Score = 601 bits (1550), Expect = 0.0
Identities = 332/336 (98%), Positives = 334/336 (99%)

Query: 1 MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSGDKKDRDYGDAFVMHKETAL 60
MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSGDKKDRDYGDAFVMHKETAL
Sbjct: 1 MGDVSAVSSSGNILLPQQDEVGGLSEALKKAVEKHKTEYSGDKKDRDYGDAFVMHKETAL 60

Query: 61 PVLLAAWRHGAPAKSEHHNGNVSGLHHNGKGELRIAEKLLKVTAEKSVGLISAEAKVDKS 120
P+LLAAWRHGAPAKSEHHNGNVSGLHHNGK ELRIAEKLLKVTAEKSVGLISAEAKVDKS
Sbjct: 61 PLLLAAWRHGAPAKSEHHNGNVSGLHHNGKSELRIAEKLLKVTAEKSVGLISAEAKVDKS 120

Query: 121 AALLSPKNRPLESVSGKKLSADLKAVESVSEVTDNATGISDDNIKALPGDNKAIAGEGVR 180
AALLS KNRPLESVSGKKLSADLKAVESVSEVTDNATGISDDNIKALPGDNKAIAGEGVR
Sbjct: 121 AALLSSKNRPLESVSGKKLSADLKAVESVSEVTDNATGISDDNIKALPGDNKAIAGEGVR 180

Query: 181 KEGAPLARDVAPARMAAANTGKPDDKDHKKVKDVSQLPLQPTTIADLSQLTGGDEKMPLA 240
KEGAPLARDVAPARMAAANTGKP+DKDHKKVKDVSQLPLQPTTIADLSQLTGGDEKMPLA
Sbjct: 181 KEGAPLARDVAPARMAAANTGKPEDKDHKKVKDVSQLPLQPTTIADLSQLTGGDEKMPLA 240

Query: 241 AQSKPMMTIFPTADGVKGEDSSLTYRFQRWGNDYSVNIQARQAGEFSLIPSNTQVEHRLH 300
AQSKPMMTIFPTADGVKGEDSSLTYRFQRWGNDYSVNIQARQAGEFSLIPSNTQVEHRLH
Sbjct: 241 AQSKPMMTIFPTADGVKGEDSSLTYRFQRWGNDYSVNIQARQAGEFSLIPSNTQVEHRLH 300

Query: 301 DQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA 336
DQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA
Sbjct: 301 DQWQNGNPQRWHLTRDDQQNPQQQQHRQQSGEEDDA 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2751SSPAMPROTEIN1672e-56 Salmonella surface presentation of antigen gene typ...
		>SSPAMPROTEIN#Salmonella surface presentation of antigen gene type M

signature.
Length = 147

Score = 167 bits (423), Expect = 2e-56
Identities = 141/147 (95%), Positives = 143/147 (97%)

Query: 1 MHSLTRIKVLQRRCTVFHSQCESILLRYQDEDRGLQAEEEAILEQIAGLKLLLDTLRAEN 60
MHSLTRIKVLQRRCTVFHSQCESILLRYQDEDR LQ EEEAI+EQIAGLKLLLDTLRAEN
Sbjct: 1 MHSLTRIKVLQRRCTVFHSQCESILLRYQDEDRRLQVEEEAIVEQIAGLKLLLDTLRAEN 60

Query: 61 RQLSREEIYTLLRKQSIVRRQIKDLELQIIQIQEKRSELEKKREEFQKKSKYWLRKEGNY 120
RQLSREEIY LLRKQSIVRRQIKDLELQIIQIQEKRSELEKKREEFQ+KSKYWLRKEGNY
Sbjct: 61 RQLSREEIYALLRKQSIVRRQIKDLELQIIQIQEKRSELEKKREEFQEKSKYWLRKEGNY 120

Query: 121 QRWIIRQKRHYIQREIQQEEAESEEII 147
QRWIIRQKR YIQREIQQEEAESEEII
Sbjct: 121 QRWIIRQKRLYIQREIQQEEAESEEII 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2753SSPAKPROTEIN2057e-72 Invasion protein B family signature.
		>SSPAKPROTEIN#Invasion protein B family signature.

Length = 133

Score = 205 bits (523), Expect = 7e-72
Identities = 43/133 (32%), Positives = 76/133 (57%)

Query: 1 MQHLDIAELVRSALEVSGCDPSLIGGIDSHSTIVLDLFALPSICISVKDDDVWIWAQLGA 60
M ++++ +LVR +L GC PS+I +DSHS I + L ++P+I I++ ++ V +WA A
Sbjct: 1 MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAINIALVNEQVMLWANFDA 60

Query: 61 GSMVVLQQRAYEILMTIMEGCHFARGGQLLLGEQNGELTLKALVHPDFLSDGEKFSTALN 120
S V LQ AY IL ++ ++ + L + L L+ ++ D++ DG F+ L+
Sbjct: 61 PSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRVVIKDDYVHDGIVFAEILH 120

Query: 121 GFYNYLEVFSRSL 133
FY +E+ + L
Sbjct: 121 EFYQRMEILNGVL 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2755INVEPROTEIN6040.0 Salmonella/Shigella invasion protein E (InvE) signat...
		>INVEPROTEIN#Salmonella/Shigella invasion protein E (InvE)

signature.
Length = 372

Score = 604 bits (1557), Expect = 0.0
Identities = 372/372 (100%), Positives = 372/372 (100%)

Query: 1 MIPGSTSGISFSRILSRQASHQDATQHTDAQQAEIQQAAEDSSPGAEVQKFVQSTDEMSA 60
MIPGSTSGISFSRILSRQASHQDATQHTDAQQAEIQQAAEDSSPGAEVQKFVQSTDEMSA
Sbjct: 1 MIPGSTSGISFSRILSRQASHQDATQHTDAQQAEIQQAAEDSSPGAEVQKFVQSTDEMSA 60

Query: 61 ALAQFRNRRDYEKKSSNLSNSFERVLEDEALPKAKQILKLISVHGGALEDFLRQARSLFP 120
ALAQFRNRRDYEKKSSNLSNSFERVLEDEALPKAKQILKLISVHGGALEDFLRQARSLFP
Sbjct: 61 ALAQFRNRRDYEKKSSNLSNSFERVLEDEALPKAKQILKLISVHGGALEDFLRQARSLFP 120

Query: 121 DPSDLVLVLRELLRRKDLEEIVRKKLESLLKHVEEQTDPKTLKAGINCALKARLFGKTLS 180
DPSDLVLVLRELLRRKDLEEIVRKKLESLLKHVEEQTDPKTLKAGINCALKARLFGKTLS
Sbjct: 121 DPSDLVLVLRELLRRKDLEEIVRKKLESLLKHVEEQTDPKTLKAGINCALKARLFGKTLS 180

Query: 181 LKPGLLRASYRQFIQSESHEVEIYSDWIASYGYQRRLVVLDFIEGSLLTDIDANDASCSR 240
LKPGLLRASYRQFIQSESHEVEIYSDWIASYGYQRRLVVLDFIEGSLLTDIDANDASCSR
Sbjct: 181 LKPGLLRASYRQFIQSESHEVEIYSDWIASYGYQRRLVVLDFIEGSLLTDIDANDASCSR 240

Query: 241 LEFGQLLRRLTQLKMLRSADLLFVSTLLSYSFTKAFNAEESSWLLLMLSLLQQPHEVDSL 300
LEFGQLLRRLTQLKMLRSADLLFVSTLLSYSFTKAFNAEESSWLLLMLSLLQQPHEVDSL
Sbjct: 241 LEFGQLLRRLTQLKMLRSADLLFVSTLLSYSFTKAFNAEESSWLLLMLSLLQQPHEVDSL 300

Query: 301 LADIIGLNALLLSHKEHASFLQIFYQVCKAIPSSLFYEEYWQEELLMALRSMTDIAYKHE 360
LADIIGLNALLLSHKEHASFLQIFYQVCKAIPSSLFYEEYWQEELLMALRSMTDIAYKHE
Sbjct: 301 LADIIGLNALLLSHKEHASFLQIFYQVCKAIPSSLFYEEYWQEELLMALRSMTDIAYKHE 360

Query: 361 MAEQRRTIEKLS 372
MAEQRRTIEKLS
Sbjct: 361 MAEQRRTIEKLS 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2756TYPE3OMGPROT5760.0 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 576 bits (1486), Expect = 0.0
Identities = 169/540 (31%), Positives = 271/540 (50%), Gaps = 57/540 (10%)

Query: 4 HILLARVLACAALVLVAPGYSSE----KIPVTGSGFVAKDDSLRTFFDAMALQLKEPVIV 59
H RVL L+L + ++ E IP +VAK +SLR V+V
Sbjct: 6 HSFFKRVLTGTLLLLSSYSWAQELDWLPIPYV---YVAKGESLRDLLTDFGANYDATVVV 62

Query: 60 SKMAARKKITGNFEFHDPNALLEKLSLQLGLIWYFDGQAIYIYDASEMRNAVVSLRNVSL 119
S K++G FE +P L+ ++ L+WY+DG +YI+ SE+ + ++ L+
Sbjct: 63 SD-KINDKVSGQFEHDNPQDFLQHIASLYNLVWYYDGNVLYIFKNSEVASRLIRLQESEA 121

Query: 120 NEFNNFLKRSGLYNKNYPLRGDNRKGTFYVSGPPVYVDMVVNAATMMDKQND--GIELGR 177
E L+RSG++ + R D YVSGPP Y+++V A +++Q + G
Sbjct: 122 AELKQALQRSGIWEPRFGWRPDASNRLVYVSGPPRYLELVEQTAAALEQQTQIRSEKTGA 181

Query: 178 QKIGVMRLNNTFVGDRTYNLRDQKMVIPGIATAIERLLQGEEQPLGNIVSSEPPAMPAFS 237
I + L DRT + RD ++ PG+AT ++R+L + + P
Sbjct: 182 LAIEIFPLKYASASDRTIHYRDDEVAAPGVATILQRVLSDATIQQVTVDNQRIP------ 235

Query: 238 ANGEKGKAANYAGGMSLQEALKQNAAAGNIKIVAYPDTNSLLVKGTAEQVHFIEMLVKAL 297
Q A + +A A ++ A P N+++V+ + E++ + L+ AL
Sbjct: 236 -----------------QAATRASAQA---RVEADPSLNAIIVRDSPERMPMYQRLIHAL 275

Query: 298 DVAKRHVELSLWIVDLNKSDLERLGTSWSGSI-----------TIGDKLGVSLNQSSIST 346
D +E++L IVD+N L LG W I T GD+ ++ N + S
Sbjct: 276 DKPSARIEVALSIVDINADQLTELGVDWRVGIRTGNNHQVVIKTTGDQSNIASNGALGSL 335

Query: 347 LDG---SRFIAAVNALEEKKQATVVSRPVLLTQENVPAIFDNNRTFYTKLIGERNVALEH 403
+D +A VN LE + A VVSRP LLTQEN A+ D++ T+Y K+ G+ L+
Sbjct: 336 VDARGLDYLLARVNLLENEGSAQVVSRPTLLTQENAQAVIDHSETYYVKVTGKEVAELKG 395

Query: 404 VTYGTMIRVLPRFSADG---QIEMSLDIEDGNDKTPQSDTTTSVDALPEVGRTLISTIAR 460
+TYGTM+R+ PR G +I ++L IEDGN Q ++ ++ +P + RT++ T+AR
Sbjct: 396 ITYGTMLRMTPRVLTQGDKSEISLNLHIEDGN----QKPNSSGIEGIPTISRTVVDTVAR 451

Query: 461 VPHGKSLLVGGYTRDANTDTVQSIPFLGKLPLIGSLFRYSSKNKSNVVRVFMIEPKEIVD 520
V HG+SL++GG RD + + +P LG +P IG+LFR S+ VR+F+IEP+ I +
Sbjct: 452 VGHGQSLIIGGIYRDELSVALSKVPLLGDIPYIGALFRRKSELTRRTVRLFIIEPRIIDE 511


89SPA2809SPA2816N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA28090210.174843enolase
SPA2810-1130.552329CTP synthetase
SPA28110110.763117hypothetical protein
SPA28122120.398635fimbrial subunit
SPA2813113-1.477546outer membrane usher protein
SPA2814425-5.831165periplasmic fimbrial chaperone
SPA2815532-7.192197fimbrial subunit
SPA2816019-4.320321fimbrial subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2809ANTHRAXTOXNA290.036 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 29.3 bits (65), Expect = 0.036
Identities = 31/132 (23%), Positives = 51/132 (38%), Gaps = 9/132 (6%)

Query: 211 GYAPNLGSNAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLA-----GEGN 265
P L N + A+ +E K YE+GK I+L + + ++ + +
Sbjct: 147 RETPKLIINIKDYAINSEQSKEVYYEIGKGISLDIISKDKSLDPEFLNLIKSLSDDSDSS 206

Query: 266 KAFTSEEFTHFLEELTKQYPIVSIEDGLDESDW---DGFAYQTKVLG-DKIQLVGDDLFV 321
S++F LE K I I++ L E F+Y ++L D+F
Sbjct: 207 DLLFSQKFKEKLELNNKSIDINFIKENLTEFQHAFSLAFSYYFAPDHRTVLELYAPDMFE 266

Query: 322 TNTKILKEGIEK 333
K+ K G EK
Sbjct: 267 YMNKLEKGGFEK 278


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2813PF005777020.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 702 bits (1814), Expect = 0.0
Identities = 216/864 (25%), Positives = 369/864 (42%), Gaps = 68/864 (7%)

Query: 5 ASPYSASGKDIEFNTDFLDVKNRDNVNIAQFSRKGFILPGVYLLQIKINGQTLPQEFPVN 64
A+ S ++ FN FL + ++++F + PG Y + I +N + V
Sbjct: 37 AAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATR-DVT 95

Query: 65 WVIPEHDPQGSEVCAEPELVTQLGIKPELAEKLVWITHGERQCLAPDSL-KGMDFQADLG 123
+ QG C + +G+ + + + C+ S+ Q D+G
Sbjct: 96 FN-TGDSEQGIVPCLTRAQLASMGLNTASVSGMNLL--ADDACVPLTSMIHDATAQLDVG 152

Query: 124 HSTLLVNLPQAYMEYSDVDWDPPARWDNGIPGIILDYNINNQLRHDQESGSEEQSISGNG 183
L + +PQA+M + PP WD GI +L+YN + ++ G+ N
Sbjct: 153 QQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNS-HYAYLNL 211

Query: 184 TLGANLGAWRLRADWQASYDHRDDDENTSTLHDQSWSRYYAYRALPTLGAKLTLGESYLQ 243
G N+GAWRLR + SY+ D + + R + L ++LTLG+ Y Q
Sbjct: 212 QSGLNIGAWRLRDNTTWSYNSSDSSSGSKN--KWQHINTWLERDIIPLRSRLTLGDGYTQ 269

Query: 244 SDVFDSFNYIGASVVSDDQMLPPKLRGYAPEIVGIARSNAKVKVSWQGRVLYETQVPAGP 303
D+FD N+ GA + SDD MLP RG+AP I GIAR A+V + G +Y + VP GP
Sbjct: 270 GDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGP 329

Query: 304 FRIQDLNQ-SVSGTLHVTVEEQNGQTQEFDVNTASVPFLTRPGMVRYKMALGRPQDWDHH 362
F I D+ SG L VT++E +G TQ F V +SVP L R G RY + G + +
Sbjct: 330 FTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQ 389

Query: 363 PITGTFASAEASWGVTNGWSLYGGAIGESNYQAVALGSGKDLGVVGAVAVDITHSIAHMP 422
F + G+ GW++YGG Y+A G GK++G +GA++VD+T + + +P
Sbjct: 390 QEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLP 449

Query: 423 QDDGFDGETLQGNSYRISYSRDFDEIDSRLTFAGYRFSEKNFMSMSDYLDAKT--YHHLN 480
D G S R Y++ +E + + GYR+S + + +D ++ Y+
Sbjct: 450 DD-----SQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIET 504

Query: 481 A-----------------GHEKERYTVTYNQNFREQGMSAYFSYSRSTFWDSPDQS-NYN 522
+++ + +T Q + Y S S T+W + + +
Sbjct: 505 QDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTS-TLYLSGSHQTYWGTSNVDEQFQ 563

Query: 523 LSLSWYFDLGSIKNLSASLNGYRSEYNGDKDDGVYISLSVPWG------------NDSIS 570
L+ F+ + + S + ++ + +D + +++++P+ + S S
Sbjct: 564 AGLNTAFEDIN---WTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASAS 620

Query: 571 YNGT-FNGSQHRNQLGYSGH--SQNGDNWQLHVG-----QDEQGAQADGYYSHQGALTDI 622
Y+ + + N G G N ++ + G G+ +++G +
Sbjct: 621 YSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNA 680

Query: 623 DLSADYEEGSYRSLGMSLRGGMTLTTQGGALHRGSLAGSTRLLVDTDGIADVPVSGNGSP 682
++ + + + L + GG+ G L + T +LV G D V N +
Sbjct: 681 NIGYSHSDD-IKQLYYGVSGGVLAHANGVTLGQPL--NDTVVLVKAPGAKDAKV-ENQTG 736

Query: 683 TSTNIFGKAVIADVGSYSRSLARIDLNKLPEKAEATKSVVQITLTEGAIGYRHFDVVSGE 742
T+ G AV+ Y + +D N L + + +V + T GAI F G
Sbjct: 737 VRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGI 796

Query: 743 KMMAVFRLADGDFPPFGAEVKNERQQQLGLVADDGNAWLAGVKAGETLKVFW--DGAAQC 800
K++ + PFGA V +E Q G+VAD+G +L+G+ ++V W + A C
Sbjct: 797 KLLMTLT-HNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHC 855

Query: 801 EA--SLPPTFTPELLANALLLPCK 822
A LPP +LL L C+
Sbjct: 856 VANYQLPPESQQQLL-TQLSAECR 878


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2815FIMBRIALPAPF342e-04 Escherichia coli: P pili tip fibrillum papF protein...
		>FIMBRIALPAPF#Escherichia coli: P pili tip fibrillum papF protein

signature.
Length = 167

Score = 33.5 bits (76), Expect = 2e-04
Identities = 36/144 (25%), Positives = 61/144 (42%), Gaps = 26/144 (18%)

Query: 39 PPCTVGGAS---VEFGDVLTTKVGDVSQTKPVGYSLNCDGRASDYLKLQIQGTTTTISGE 95
PPCT+ V+FG++ V + S++C + S L +++ G T +
Sbjct: 32 PPCTINNGQNIVVDFGNINPEHVDNSRGEVTKNISISCPYK-SGSLWIKVTGNTMGVGQN 90

Query: 96 QVLQTSVQGLGIRIQQ-------------AGNKQLVPVGI-TDWLNFTLSGSNGPELEAV 141
VL T++ GI + Q +GN V G+ T FT + +V
Sbjct: 91 NVLATNITHFGIALYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFT--------SV 142

Query: 142 PVKEPTTQLAGGDFNASATLVVDY 165
P + + L GGDF +A++ + Y
Sbjct: 143 PFRNGSGILNGGDFRTTASMSMIY 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA2816FIMBRIALPAPF376e-06 Escherichia coli: P pili tip fibrillum papF protein...
		>FIMBRIALPAPF#Escherichia coli: P pili tip fibrillum papF protein

signature.
Length = 167

Score = 37.4 bits (86), Expect = 6e-06
Identities = 43/166 (25%), Positives = 71/166 (42%), Gaps = 20/166 (12%)

Query: 5 LILTLLITRFAC-AD-NLTFHGKLINPPACTINNGETLEVSFGSVIIDNIDGVNYLTEIP 62
L ++LL+T A AD + G + PP CTINNG+ + V FG++ +++D N E+
Sbjct: 6 LFISLLLTSVAVLADVQINIRGNVYIPP-CTINNGQNIVVDFGNINPEHVD--NSRGEVT 62

Query: 63 WTLTCDSSFRDDALTFTLSYLGTATPYSAKALTTNVPGLGIELQQNGTVFPPGT------ 116
++ ++ +L ++ T L TN+ GI L Q + P T
Sbjct: 63 KNISISCPYKSGSLWIKVTG-NTMGVGQNNVLATNITHFGIALYQGKGMSTPLTLGNGSG 121

Query: 117 -------SLTINESSLPTLKAVPVKQPGKEPAEGDFEAFATLQVDY 155
L S+ T +VP + GDF A++ + Y
Sbjct: 122 NGYRVTAGLDTARSTF-TFTSVPFRNGSGILNGGDFRTTASMSMIY 166


90SPA3252SPA3259N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3252-115-1.873897Fis DNA-binding protein
SPA3254-116-1.771649hypothetical protein
SPA3255-116-1.608755diguanylate cyclase/phosphodiesterase
SPA3256-118-2.575197transcriptional repressor for envCD (acrEF)
SPA3257017-1.844348transmembrane protein
SPA3258-118-2.360843RND family, multidrug transport protein,
SPA3259021-2.049336lipoprotein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3252DNABINDNGFIS1573e-54 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 157 bits (399), Expect = 3e-54
Identities = 98/98 (100%), Positives = 98/98 (100%)

Query: 1 MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQ 60
MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQ
Sbjct: 1 MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQ 60

Query: 61 PLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN 98
PLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN
Sbjct: 61 PLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN 98


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3256HTHTETR1284e-39 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 128 bits (322), Expect = 4e-39
Identities = 81/216 (37%), Positives = 129/216 (59%), Gaps = 3/216 (1%)

Query: 1 MAKKTKADALKTRQHLIETAIAQFALRGVANTTLNDIADAADVTRGAIYWHFENKTQLFN 60
MA+KTK +A +TRQH+++ A+ F+ +GV++T+L +IA AA VTRGAIYWHF++K+ LF+
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EVW-LQQPPLRELIQDRLTGCWNDNPLQDPREKFIAALQYIAAVPRQQALMQILYHKCEF 119
E+W L + + EL + +PL RE I L+ R++ LM+I++HKCEF
Sbjct: 61 EIWELSESNIGELELEYQAKF-PGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEF 119

Query: 120 HNGM-ISEQAIREKMGFHHQSLLEVLQRCMDKKLISGSLDLDVILIILHGSFSGIVKNWL 178
M + +QA R + + + L+ C++ K++ L II+ G SG+++NWL
Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179

Query: 179 MNPTSYDLYKQAPALVDNVLKMLSPDGSVRQLMPNE 214
P S+DL K+A V +L+M ++R NE
Sbjct: 180 FAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3257RTXTOXIND432e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 42.5 bits (100), Expect = 2e-06
Identities = 24/137 (17%), Positives = 48/137 (35%), Gaps = 15/137 (10%)

Query: 98 ATYQADYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADA-RQADAAV 156
+ K +L + E+ A + Q + I D RQ +
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLV----------TQLFKNEILDKLRQTTDNI 311

Query: 157 VAAKAAVESARINLAYTKVTSPISGRIGKSNV-TEGALVTNGQSTELATVQQLDPIYVDV 215
+ + + +P+S ++ + V TEG +VT + T + V + D + V
Sbjct: 312 GLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAE-TLMVIVPEDDTLEVTA 370

Query: 216 TQSSND--FMRLKQSVE 230
+ D F+ + Q+
Sbjct: 371 LVQNKDIGFINVGQNAI 387



Score = 37.1 bits (86), Expect = 1e-04
Identities = 22/127 (17%), Positives = 41/127 (32%), Gaps = 13/127 (10%)

Query: 46 TAPLAVTTELPGR-TSAFRIAEVRPQVSGIVLKRNFTEGSDVEAGQSLYQIDPATYQADY 104
+ + G+ T + R E++P + IV + EG V G L ++ +AD
Sbjct: 77 LGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEAD- 135

Query: 105 DSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVVAAKAAVE 164
K++++ A L RY L E ++ +
Sbjct: 136 ------TLKTQSSLLQARLEQTRYQIL-----SRSIELNKLPELKLPDEPYFQNVSEEEV 184

Query: 165 SARINLA 171
+L
Sbjct: 185 LRLTSLI 191


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3258ACRIFLAVINRP13910.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1391 bits (3602), Expect = 0.0
Identities = 917/1032 (88%), Positives = 974/1032 (94%)

Query: 1 MANFFIRRPIFAWVLAIILMMAGALAIMQLPVAQYPTIAPPAVSISATYPGADAQTVQDT 60
MANFFIRRPIFAWVLAIILMMAGALAI+QLPVAQYPTIAPPAVS+SA YPGADAQTVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120
VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGISVEKSSSSFLMVAGFVSDNPNTTQDDISDYVASNIKDSISRLNGVGDVQLFGA 180
EVQQQGISVEKSSSS+LMVAGFVSDNP TTQDDISDYVASN+KD++SRLNGVGDVQLFGA
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWLDANLLNKYQLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRL 240
QYAMRIWLDA+LLNKY+LTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 KDPEEFGKVTLRVNTDGSVVHLKDVARIELGGENYNVVARINGKPASGLGIKLATGANAL 300
K+PEEFGKVTLRVN+DGSVV LKDVAR+ELGGENYNV+ARINGKPA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTATAIKAKLAELQPFFPQGMKVVYPYDTTPFVKISIHEVVKTLFEAIILVFLVMYLFLQ 360
DTA AIKAKLAELQPFFPQGMKV+YPYDTTPFV++SIHEVVKTLFEAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NIRATLIPTIAVPVVLLGTFAVLAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
N+RATLIPTIAVPVVLLGTFA+LAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 MEDNLSPREATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480
MED L P+EATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATLLKPVSAEHHEKKSGFFGWFNTRFDHSVNHYTNSVSGIVRNTGRY 540
SVLVALILTPALCATLLKPVSAEHHE K GFFGWFNT FDHSVNHYTNSV I+ +TGRY
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 541 LIIYLLIVVGMAVLFLRLPTSFLPEEDQGVFLTMIQLPSGATQERTQKVLDQVTHYYLNN 600
L+IY LIV GM VLFLRLP+SFLPEEDQGVFLTMIQLP+GATQERTQKVLDQVT YYL N
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 601 EKANVESVFTVNGFSFSGQGQNSGMAFVSLKPWEERNGEENSVEAVIARATRAFSQIRDG 660
EKANVESVFTVNGFSFSGQ QN+GMAFVSLKPWEERNG+ENS EAVI RA +IRDG
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 661 LVFPFNMPAIVELGTATGFDFELIDQGGLGHDALTKARNQLLGMVAKHPDLLVRVRPNGL 720
V PFNMPAIVELGTATGFDFELIDQ GLGHDALT+ARNQLLGM A+HP LV VRPNGL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 721 EDTPQFKLDVDQEKAQALGVSLSDINETISAALGGYYVNDFIDRGRVKKVYVQADAQFRM 780
EDT QFKL+VDQEKAQALGVSLSDIN+TIS ALGG YVNDFIDRGRVKK+YVQADA+FRM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 781 LPGDINNLYVRSANGEMVPFSTFSSARWIYGSPRLERYNGMPSMELLGEAAPGRSTGEAM 840
LP D++ LYVRSANGEMVPFS F+++ W+YGSPRLERYNG+PSME+ GEAAPG S+G+AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 841 SLMENLASQLPNGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFSV 900
+LMENLAS+LP GIGYDWTGMSYQERLSGNQAPAL AIS +VVFLCLAALYESWSIP SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 901 MLVVPLGVVGALLAASLRGLNNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMEKEGRGLI 960
MLVVPLG+VG LLAA+L NDVYF VGLLTTIGLSAKNAILIVEFAKDLMEKEG+G++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 961 EATLEASRMRLRPILMTSLAFILGVMPLVISRGAGSGAQNAVGTGVMGGMLTATLLAIFF 1020
EATL A RMRLRPILMTSLAFILGV+PL IS GAGSGAQNAVG GVMGGM++ATLLAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1021 VPVFFVVVKRRF 1032
VPVFFVV++R F
Sbjct: 1021 VPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3259adhesinb290.001 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 29.0 bits (65), Expect = 0.001
Identities = 14/68 (20%), Positives = 26/68 (38%), Gaps = 10/68 (14%)

Query: 1 MKR---LIPVALLTTLLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGG 57
MK+ L+ + L LA C+ + +V TN+ + T++ IAG
Sbjct: 1 MKKCRFLVLLLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKN-------IAGD 53

Query: 58 AAAVAGLT 65
+ +
Sbjct: 54 KINLHSIV 61


91SPA3308SPA3312N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3308447-2.395251Type III leader peptidase
SPA3309652-2.407714bacterioferritin
SPA3310755-1.450607bacterioferritin-associated ferredoxin
SPA3311754-0.909952elongation factor Tu
SPA3312443-0.845562elongation factor G
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3308PREPILNPTASE1413e-44 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 141 bits (356), Expect = 3e-44
Identities = 59/143 (41%), Positives = 86/143 (60%), Gaps = 2/143 (1%)

Query: 4 ALPFLIFYASFSLLLGIYDARTGLLPDRFTCPLLWGGLLYHQICLPERLPDALWGAIAGY 63
L L+ + L D LLPD+ T PLLWGGLL++ + L DA+ GA+AGY
Sbjct: 134 TLAALLLT-WVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMAGY 192

Query: 64 GGFALIYWGYRLRYQKEGLGYGDVKYLAALGAWHCWETLPLLVFLAAMLACGRFGVALLV 123
+YW ++L KEG+GYGD K LAALGAW W+ LP+++ L++++ G+ L++
Sbjct: 193 LVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAF-MGIGLIL 251

Query: 124 RGKSALINPLPFGPWLAVAGFIT 146
P+PFGP+LA+AG+I
Sbjct: 252 LRNHHQSKPIPFGPYLAIAGWIA 274


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3309HELNAPAPROT371e-05 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 36.8 bits (85), Expect = 1e-05
Identities = 18/103 (17%), Positives = 43/103 (41%), Gaps = 10/103 (9%)

Query: 44 EYHESIDEMKHADKYIERILFLEGIPN--LQDLGKL------GIGEDVEEMLRSDLRLEL 95
E ++ E D ER+L + G P +++ + G EM+++ +
Sbjct: 52 ELYDHAAE--TVDTIAERLLAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYK 109

Query: 96 EGAKDLREAIAYADSVHDYVSRDMMIEILADEEGHIDWLETEL 138
+ + + + I A+ D + D+ + ++ + E + L + L
Sbjct: 110 QISSESKFVIGLAEENQDNATADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3311TCRTETOQM803e-18 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 79.5 bits (196), Expect = 3e-18
Identities = 57/198 (28%), Positives = 87/198 (43%), Gaps = 13/198 (6%)

Query: 13 VNVGTIGHVDHGKTTLTAAI------TTVLAKTYGGAARAFDQIDNAPEEKARGITINTS 66
+N+G + HVD GKTTLT ++ T L G R DN E+ RGITI T
Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRT----DNTLLERQRGITIQTG 59

Query: 67 HVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQV 126
+ +D PGH D++ + + +DGAIL+++A DG QTR R++
Sbjct: 60 ITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKM 119

Query: 127 GVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWE 186
G+P I F+NK D + L V +++E LS + + +W+
Sbjct: 120 GIP-TIFFINKIDQNGID--LSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWD 176

Query: 187 AKIIELAGFLDSYIPEPE 204
I L+ Y+
Sbjct: 177 TVIEGNDDLLEKYMSGKS 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3312TCRTETOQM6160.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 616 bits (1591), Expect = 0.0
Identities = 178/698 (25%), Positives = 305/698 (43%), Gaps = 81/698 (11%)

Query: 9 RYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAAT 68
+ NIG+ AH+DAGKTT TE +L+ +G ++G V G D E++RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 69 TAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWR 128
+ W ++NIIDTPGH+DF EV RS+ VLDGA+++ A GVQ Q+ ++
Sbjct: 62 SFQWEN-------TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFH 114

Query: 129 QANKYKVPRIAFVNKMDRMGANFLKVVGQIKTRLGANPVPLQLAIGAEEGFTGVVDLVKM 188
K +P I F+NK+D+ G + V IK +L A V Q V M
Sbjct: 115 ALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ----------KVELYPNM 164

Query: 189 KAINWNDADQGVTFEYEDIPADMQDLANEWHQNLIESAAEASEELMEKYLGGEELTEEEI 248
N+ +++Q ++ E +++L+EKY+ G+ L E+
Sbjct: 165 CVTNFTESEQ------------------------WDTVIEGNDDLLEKYMSGKSLEALEL 200

Query: 249 KQALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDDGKDTPAE 308
+Q R N + V GSA N G+ +++ + + S
Sbjct: 201 EQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH----------------- 243

Query: 309 RHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKTARERFGRIVQMHA 368
FKI L + R+YSGV++ D+V S K + + +
Sbjct: 244 ---RGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEK-EKIKITEMYTSIN 299

Query: 369 NKREEIKEVRAGDIAAAIG----LKDVTTGDTLCDPENPIILERMEFPEPVISIAVEPKT 424
+ +I + +G+I L V GDT P+ ER+E P P++ VEP
Sbjct: 300 GELCKIDKAYSGEIVILQNEFLKLNSV-LGDTKLLPQR----ERIENPLPLLQTTVEPSK 354

Query: 425 KADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVEANVG 484
+E + AL ++ DP R + D +++ I++ +G++ +++ ++ +++VE +
Sbjct: 355 PQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIK 414

Query: 485 KPQVAYREAIRAKVTDIEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGV 544
+P V Y E K E + + + + + PL GS G ++ + + G
Sbjct: 415 EPTVIYMERPLKKA---EYTIHIEVPPNPFWASIGLSVSPLPLGS---GMQYESSVSLGY 468

Query: 545 IPGEYIPAVDKGIQEQLKSGPLAGYPVVDLGVRLHFGSYHDVDSSELAFKLAASIAFKEG 604
+ + AV +GI+ + G L G+ V D + +G Y+ S+ F++ A I ++
Sbjct: 469 LNQSFQNAVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQV 527

Query: 605 FKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMF 664
KKA LLEP + ++ P+E D + + + + V + E+P +
Sbjct: 528 LKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQ 587

Query: 665 GYATQLRSLTKGRASYTMEFLKYDDAPNNVAQAVIEAR 702
Y + L T GR+ E Y + V + R
Sbjct: 588 EYRSDLTFFTNGRSVCLTELKGYHVT---TGEPVCQPR 622


92SPA3318SPA3333N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3318-113-0.738158hypothetical protein
SPA3319-1120.555578FKBP-type peptidyl-prolyl isomerase
SPA33201132.395800hypothetical protein
SPA33210142.037905FKBP-type peptidyl-prolyl cis-trans isomerase
SPA33220151.914172hypothetical protein
SPA33230151.915027glutathione-regulated potassium-efflux system
SPA3324-1151.842069oxidoreductase
SPA33250171.699107ABC-transporter ATP-binding protein
SPA3327-114-0.546767hypothetical protein
SPA3328-1120.549098hydrolase
SPA3329-2120.861719hypothetical protein
SPA3330-1121.085439phosphoribulokinase
SPA3331-1111.164842hypothetical protein
SPA3332-1111.169198cyclic AMP receptor protein,catabolite gene
SPA3333-1121.259704inner membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3318ACRIFLAVINRP290.020 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.0 bits (65), Expect = 0.020
Identities = 14/62 (22%), Positives = 29/62 (46%), Gaps = 1/62 (1%)

Query: 160 ASSVEDLVTQTLEFTIEEVNADRNV-SNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI 218
A +V+D VTQ +E + ++ + S + + + L + D A QV ++L +
Sbjct: 54 AQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQL 113

Query: 219 SK 220
+
Sbjct: 114 AT 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3319INFPOTNTIATR1282e-38 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 128 bits (323), Expect = 2e-38
Identities = 80/226 (35%), Positives = 122/226 (53%), Gaps = 9/226 (3%)

Query: 28 AAKPAATADSKAAFKNDDQKAAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAF 87
A A A + D K +Y++GA LG K + GI ++ D L G+QD
Sbjct: 14 AMSTAMAATDATSLTTDKDKLSYSIGADLG-------KNFKNQGIDINPDVLAKGMQDGM 66

Query: 88 A-DKSKLSDQEIEQTLQTFEARVKSAAQAKMEKDAADNEAKGKTFRDAFAKEKGVKTSST 146
+ + L++++++ L F+ + + A+ K A +N+AKG F A + G+ +
Sbjct: 67 SGAQLILTEEQMKDVLSKFQKDLMAKRSAEFNKKAEENKAKGDAFLSANKSKPGIVVLPS 126

Query: 147 GLLYKVEKEGTGEAPKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGL 206
GL YK+ GTG P SDTV V Y GTLIDG FD++ G+P +F++ VIPGWTE L
Sbjct: 127 GLQYKIIDAGTGAKPGKSDTVTVEYTGTLIDGTVFDSTEKAGKPATFQVSQVIPGWTEAL 186

Query: 207 KNIKKGGKIKLVIPPELAYGKTGVPG-IPANSTLVFDVELLDIKPA 251
+ + G ++ +P +LAYG V G I N TL+F + L+ +K A
Sbjct: 187 QLMPAGSTWEVFVPADLAYGPRSVGGPIGPNETLIFKIHLISVKKA 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3324ISCHRISMTASE280.025 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 27.7 bits (61), Expect = 0.025
Identities = 35/138 (25%), Positives = 52/138 (37%), Gaps = 22/138 (15%)

Query: 11 YAHPESQDSVANRVLLKPAIQHNNVTVHDLYARYPDFFID--TPYEQ-----ALLREHDV 63
Y P + D N+V P + +HD+ + D F +P + L+ V
Sbjct: 9 YQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCV 68

Query: 64 IVFQH--PLYTYSCPALLKEWLDRVLSRGFASGPGGNQLVGKYWRSVITTGEPESA---- 117
Q P+ + P DR L F GPG N G Y +IT PE
Sbjct: 69 ---QLGIPVVYTAQPGSQNP-DDRALLTDFW-GPGLNS--GPYEEKIITELAPEDDDLVL 121

Query: 118 --YRYDALNRYPMSDVLR 133
+RY A R + +++R
Sbjct: 122 TKWRYSAFKRTNLLEMMR 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3325PYOCINKILLER310.019 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 30.9 bits (69), Expect = 0.019
Identities = 21/85 (24%), Positives = 33/85 (38%), Gaps = 7/85 (8%)

Query: 522 VQKQENQADDAPKENNANSAQSRKDQKRREAELRTLT---QPLRKEITRLEKEMEKLNAQ 578
+ E + A +E N N ++ RE E T + + I+ L+ M L A
Sbjct: 151 TRTAEEIGEQAVREGNINGPEAYMRFLDREMEGLTAAYNVKLFTEAISSLQIRMNTLTAA 210

Query: 579 LA----QAEEKLGDSSLYDPSRKAE 599
A A K + + + RKAE
Sbjct: 211 KASIEAAAANKAREQAAAEAKRKAE 235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3329FLGFLIH250.024 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 25.1 bits (54), Expect = 0.024
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 3/46 (6%)

Query: 3 IPWQGLAPDTLDNLIESFV---LREGTDYGEHERSLEQKVADVKRQ 45
+PW+ PD L FV E T E E SLEQ++A ++ Q
Sbjct: 5 LPWKTWTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQ 50


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3330PF07299361e-04 Fibronectin-binding protein (FBP)
		>PF07299#Fibronectin-binding protein (FBP)

Length = 219

Score = 36.0 bits (83), Expect = 1e-04
Identities = 10/46 (21%), Positives = 21/46 (45%), Gaps = 2/46 (4%)

Query: 71 PEANDFSLLEHTFIEYGQTGKGQSRKYLHTYDEAVPWNQVPGTFTP 116
P+ + + E ++ KG SRK++ ++ + + GTF
Sbjct: 112 PDMEELDMKELSY--LSWIDKGSSRKFIIAKNDKNKFVGLQGTFQS 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3333YERSSTKINASE310.014 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 31.2 bits (70), Expect = 0.014
Identities = 18/52 (34%), Positives = 27/52 (51%)

Query: 630 RPGGSGDVNILESPDMPSHGLLSTLEQHLQRIIGHLNTMHTISSMAWRQRPH 681
R G + + + SP S+ +LS +E LQRI HL+ H+ S + R H
Sbjct: 515 REGDTKNSSTEVSPYHRSNFMLSIVEPSLQRIQKHLDQTHSFSDIGSLVRAH 566


93SPA3366SPA3373N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA33660223.418678two-component sensor kinase EnvZ
SPA33671243.239213two-component response regulator OmpR
SPA3368-1202.607453transcription elongation factor GreB
SPA3369-2173.029724transcription accessory protein
SPA3370-2142.522163ferrous iron transport protein
SPA3371-2142.531621ferrous iron transport protein B
SPA3372-2102.266966hypothetical protein
SPA3373-2122.979994hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3366PF06580340.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 33.7 bits (77), Expect = 0.001
Identities = 27/188 (14%), Positives = 71/188 (37%), Gaps = 45/188 (23%)

Query: 270 INKDIEECNAIIEQFIDYLR------TGQEMPM--EMADLNSVL-------GEVIAAESG 314
I +D + ++ + +R +++ + E+ ++S L + +
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQ---- 241

Query: 315 YEREINTALQAGSIQVKMHPLSIKRAVANMVVNA--ARYGNGWIKVSSGTESHRAWFQVE 372
+E +IN A+ V++ P+ ++ V N + + G I + ++ +VE
Sbjct: 242 FENQINPAIM----DVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVE 297

Query: 373 DDGPGIKPEQRKHLFQPFVRGDSARSTSGTGLGLAIV-QRIIDNH--NGMLEIGTSERGG 429
+ G ++ TG GL V +R+ + +++ + ++G
Sbjct: 298 NTGSLALKNTKE----------------STGTGLQNVRERLQMLYGTEAQIKL-SEKQGK 340

Query: 430 LSIRAWLP 437
++ +P
Sbjct: 341 VNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3367HTHFIS986e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 98.4 bits (245), Expect = 6e-26
Identities = 39/136 (28%), Positives = 72/136 (52%), Gaps = 3/136 (2%)

Query: 6 KILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLLTRESFHLMVLDLMLPGEDGLS 65
ILV DDD +R +L + L+ G+ VR +NA + R + L+V D+++P E+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 66 ICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDYIPKPFNPRELLARIRAVL---RR 122
+ R++ +P+++++A+ + I E GA DY+PKPF+ EL+ I L +R
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 123 QANELPGAPSQEEAVI 138
+ ++L ++
Sbjct: 125 RPSKLEDDSQDGMPLV 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3371TCRTETOQM429e-06 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 41.8 bits (98), Expect = 9e-06
Identities = 42/142 (29%), Positives = 66/142 (46%), Gaps = 30/142 (21%)

Query: 1 MKKLTIGLIGNPNSGKTTLFNQL---TGARQRVGNW-AGVTV------ERKEG---QFAT 47
MK + IG++ + ++GKTTL L +GA +G+ G T ER+ G Q
Sbjct: 1 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60

Query: 48 T-----DHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLE-RN 101
T + +V ++D PG + SL +L G A LLI+ D + R
Sbjct: 61 TSFQWENTKVNIIDTPG-HMDFLAEVYRSLS-------VLDG-AILLISAKDGVQAQTRI 111

Query: 102 LYLTLQLLELGIPCIVALNMLD 123
L+ L+ ++GIP I +N +D
Sbjct: 112 LFHALR--KMGIPTIFFINKID 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3373FLGFLIH290.019 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 29.0 bits (64), Expect = 0.019
Identities = 12/41 (29%), Positives = 23/41 (56%)

Query: 234 MRIPQHKEKIMTIAERLRREGHRNGLQKGLQQGKQEGQRLA 274
+++ H++ R++GH+ G Q+GL QG ++G A
Sbjct: 47 LQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEA 87


94SPA3403SPA3409N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3403-2162.543597gamma-glutamyltranspeptidase
SPA3404-3141.902685hypothetical protein
SPA3405-3141.830065glycerophosphoryl diester phosphodiesterase
SPA3406-2172.082303sn-Glycerol-3-phosphate transport ATP-binding
SPA3407-2181.749752sn-Glycerol-3-phosphate transport system
SPA3408-2212.734628glycerol-3-phosphate transport system permease
SPA3409-3212.736945glycerol-3-phosphate-binding periplasmic
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3403NAFLGMOTY320.004 Sodium-type flagellar protein MotY precursor signature.
		>NAFLGMOTY#Sodium-type flagellar protein MotY precursor signature.

Length = 293

Score = 32.4 bits (73), Expect = 0.004
Identities = 27/82 (32%), Positives = 37/82 (45%), Gaps = 17/82 (20%)

Query: 275 RTPISGDYRGYQVFSMPPPSSGGIHIVQILNI--LENFDMKKYGF-GSADAMQIMAEAEK 331
R P+ G+ R + SMPPP G H +I N+ + FD G+ G A I++E EK
Sbjct: 77 RRPM-GETRNVSLISMPPPWRPGEHADRITNLKFFKQFD----GYVGGQTAWGILSELEK 131

Query: 332 YAYADRSEYLGDPDFVKVPWQA 353
Y P F WQ+
Sbjct: 132 GRY---------PTFSYQDWQS 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3405PF04619280.031 Dr-family adhesin
		>PF04619#Dr-family adhesin

Length = 160

Score = 27.6 bits (61), Expect = 0.031
Identities = 11/60 (18%), Positives = 21/60 (35%), Gaps = 4/60 (6%)

Query: 29 VGARYGHTMIEFDAKLSKDGEIFLLHDDNLERTSNGWGVAGELNWQD----LLRVDAGGW 84
+G ++ D + G+ FL+ D+N ++ W + D G W
Sbjct: 70 LGCDARQVALKADTDNFEQGKFFLISDNNRDKLYVNIRPTDNSAWTTDNGVFYKNDVGSW 129


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3406PF05272290.041 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.9 bits (64), Expect = 0.041
Identities = 10/29 (34%), Positives = 16/29 (55%)

Query: 33 IVMVGPSGCGKSTLLRMVAGLERVTSGDI 61
+V+ G G GKSTL+ + GL+ +
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHF 627


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3409MALTOSEBP431e-06 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 43.2 bits (101), Expect = 1e-06
Identities = 46/176 (26%), Positives = 73/176 (41%), Gaps = 17/176 (9%)

Query: 133 SGHLLSQPFNSSTPVLYYNKDAFKKAGLDPEQPPKTWQELADYTAKLRAAGMKCGYASGW 192
+G L++ P L YNKD L P PPKTW+E+ +L+A G +
Sbjct: 126 NGKLIAYPIAVEALSLIYNKD------LLP-NPPKTWEEIPALDKELKAKGKSALMFNLQ 178

Query: 193 QGWIQLENFSAWNGLPFASKNNGFDGTDAVLEF--NKPEQVKHIALLEEMNKKGDFSYVG 250
+ + +A G F +N +D D ++ K + L++ + D Y
Sbjct: 179 EPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDY-- 236

Query: 251 RKDESTEKFYNGDCAMTTASSGSLANIRQYAKFNYGVGMMPYDADIKGAPQNAIIG 306
+ F G+ AMT + +NI +K NYGV ++P KG P +G
Sbjct: 237 --SIAEAAFNKGETAMTINGPWAWSNIDT-SKVNYGVTVLP---TFKGQPSKPFVG 286


95SPA3425SPA3443N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA34251143.450185hypothetical protein
SPA34261133.321035hypothetical protein
SPA34270133.703832heavy metal-transporting ATPase
SPA34280131.301227methyl-accepting chemotaxis citrate transducer
SPA34292151.490440hypothetical protein
SPA34301141.522398hypothetical protein
SPA34310152.132261lipoprotein
SPA3432-2153.214261hypothetical protein
SPA3433-1133.429254hypothetical protein
SPA3434-1112.651422hypothetical protein
SPA34351120.566655nickel responsive regulator
SPA34360130.017216ABC-transporter ATP-binding protein
SPA3437114-1.688942ABC transporter ATP-binding protein
SPA3438327-6.557631HlyD-family secretion protein
SPA3439735-9.736229hypothetical protein
SPA3440230-7.776086aminotransferase
SPA3441019-4.895486regulatory protein
SPA3442017-4.403514DNA-binding protein
SPA3443-114-2.596421hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3425SHIGARICIN270.026 Ribosome inactivating protein family signature.
		>SHIGARICIN#Ribosome inactivating protein family signature.

Length = 289

Score = 26.7 bits (59), Expect = 0.026
Identities = 6/29 (20%), Positives = 16/29 (55%)

Query: 7 FFIIIIALIVVAASFRFVQQRREKAANEA 35
+++I AA ++F++Q+ K ++
Sbjct: 173 ALMVLIQSTSEAARYKFIEQQIGKRVDKT 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3427ACRIFLAVINRP300.039 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 30.2 bits (68), Expect = 0.039
Identities = 17/78 (21%), Positives = 34/78 (43%), Gaps = 3/78 (3%)

Query: 336 AEERRAPIERFIDRFSRIYTPVIMVIALLVTLIPPLMFDGGWQEWIYKGLTLLLIGCPCA 395
E++ P E S+I ++ + +L + P+ F GG IY+ ++ ++ A
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVS---A 477

Query: 396 LVISTPAAITSGLAAAAR 413
+ +S A+ A A
Sbjct: 478 MALSVLVALILTPALCAT 495


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3429PF012061012e-32 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 101 bits (254), Expect = 2e-32
Identities = 28/72 (38%), Positives = 42/72 (58%)

Query: 9 DHTLDALGLRCPEPVMMVRKTVRNMQTGETLLIIADDPATTRDIPGFCTFMEHDLLAQET 68
D +LDA GL CP P++ +KT+ M GE L ++A DP + +D F H+LL Q+
Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64

Query: 69 EGLPYRYLLRKA 80
E Y + L++A
Sbjct: 65 EDGTYHFRLKRA 76


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3432TCRTETA492e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 49.1 bits (117), Expect = 2e-08
Identities = 75/399 (18%), Positives = 141/399 (35%), Gaps = 34/399 (8%)

Query: 13 LRLNLRIVSIVMFNFASYLTIGLPLAVLPGYVHD--AMGFSAFWAGLIISLQYFATLLSR 70
++ N ++ I+ + IGL + VLPG + D G++++L
Sbjct: 1 MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACA 60

Query: 71 PHAGRYADVLGPKKIVVFGLCGCFLSGLGYLLADIASAWPMISLLLLGLGRVILGI-GQS 129
P G +D G + +++ L G + + Y + A L +L +GR++ GI G +
Sbjct: 61 PVLGALSDRFGRRPVLLVSLAG---AAVDYAIMATAPF-----LWVLYIGRIVAGITGAT 112

Query: 130 FAGTGSTLWGVGVVGSLHIGRVISWNGIVTYGAMAMGAPLGVLCYAWGGLQGL--ALTVM 187
A G+ + + R + M G LG L + A +
Sbjct: 113 GAVAGAYIADITDGDER--ARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALN 170

Query: 188 GVALLAVLLALPRPSVK----ANKGKPLPFRAVLGRVWLYGMALALA-----SAGFGVIA 238
G+ L LP + P + + +A +A V A
Sbjct: 171 GLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPA 230

Query: 239 TFITLFYDAK-GWDGAAFALTLFSVAFVGT---RLLFPNGINRLGGLNVAMICFGVEIIG 294
+F + + WD ++L + + + ++ RLG M+ + G
Sbjct: 231 ALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTG 290

Query: 295 LLLVGTAAMPWMAKIGVLLTGMGFSLVFPALGVVAVKAVPPQNQGAALATYTVFMDMSLG 354
+L+ A WMA ++L + PAL + + V + QG + ++
Sbjct: 291 YILLAFATRGWMAFPIMVLLA-SGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT-S 348

Query: 355 VTGPLAGLVMTWAGVPV----IYLAAAGLVAMALLLTWR 389
+ GPL + A + ++A A L + L R
Sbjct: 349 IVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3434ENTSNTHTASED300.006 Enterobactin synthetase component D signature.
		>ENTSNTHTASED#Enterobactin synthetase component D signature.

Length = 234

Score = 29.6 bits (66), Expect = 0.006
Identities = 25/93 (26%), Positives = 43/93 (46%), Gaps = 6/93 (6%)

Query: 30 RWASWLAGRVLLSRALSPL---PEMVYGEQGKPAFSAGTPLWFNLSHSGDTIALLLSDEG 86
R A LAGR+ AL + G++ +P + G L+ ++SH T ++S +
Sbjct: 46 RKAEHLAGRIAAVHALREVGVRTVPGMGDKRQPLWPDG--LFGSISHCATTALAVISRQ- 102

Query: 87 EVGCDIEVIRPRDNWRSLANAVFSLGEHAEMEA 119
+G DIE I + LA ++ E ++A
Sbjct: 103 RIGIDIEKIMSQHTATELAPSIIDSDERQILQA 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3436ABC2TRNSPORT473e-08 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 47.2 bits (112), Expect = 3e-08
Identities = 44/171 (25%), Positives = 74/171 (43%), Gaps = 7/171 (4%)

Query: 200 REREHGTVEHLLVMPVTPFEIMMAKV-WSMGLVVLVVSGLSLMLMVKGVLGVPIEGSIPL 258
R T E +L + +I++ ++ W+ L +G + +V LG + L
Sbjct: 93 RMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAG---IGVVAAALGY-TQWLSLL 148

Query: 259 FMLGV-ALSLLATISIGIFMGTIARSMPQLGLLMILVLLPLQMLSGGSTPRESMPQAVQD 317
+ L V AL+ LA S+G+ + +A S LV+ P+ LSG P + +P Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 318 IMLTMPTTHFVSLAQAILYRGAGLSIVWPQFLTLLAIGGVFFL-IALLRFR 367
+P +H + L + I+ + + + I FFL ALLR R
Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTALLRRR 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3438RTXTOXIND779e-18 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 77.2 bits (190), Expect = 9e-18
Identities = 70/409 (17%), Positives = 135/409 (33%), Gaps = 82/409 (20%)

Query: 4 HLVWWGAGILVAVAAIAWWMLRPAGIPEGFAASNGRI--EATEVDIATKIAGRIDTILVS 61
LV + + V A +L E A +NG++ +I + I+V
Sbjct: 58 RLVAY-FIMGFLVIAFILSVLGQV---EIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 62 EGQFVRQGEVLAKMDTRV----------------LQEQRLEAI----------------- 88
EG+ VR+G+VL K+ L++ R + +
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 89 -------------------AQIKEAESAVAAARALLEQRQSEMRAAQSVVKQREAELDSV 129
Q ++ L+++++E + + + E
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 130 SKRHVRSRSLSQRGAVSVQQLDDDRAAAESARAALETAKAQVSAAKAAIEAARTSIIQ-- 187
R SL + A++ + + A L K+Q+ ++ I +A+
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 188 -----------AQTRVEAAQATERRIVADID--DSELKAPRDGRV-QYRVAEPGEVLSAG 233
QT T + S ++AP +V Q +V G V++
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 234 GRVLNMVDLSDVY-MTFFLPTEQAGLLKIGGEARLVLDAAPDLRIPATISFVASVAQFTP 292
++ +V D +T + + G + +G A + ++A P R V V
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYG---YLVGKVKNINL 410

Query: 293 KTVETHDERLKLMFRVKARIPPELLRQHLEYV--KTGLPGMAWVRLDER 339
+E D+RL L+F V I L + + +G+ A ++ R
Sbjct: 411 DAIE--DQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMR 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3439TCRTETB300.016 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 30.2 bits (68), Expect = 0.016
Identities = 19/109 (17%), Positives = 45/109 (41%), Gaps = 10/109 (9%)

Query: 226 FAAFSIFATISFYQGSSYLVPY-LSDVYGMTAEHAGIIGMIRAYVLAILIAPVVGLLADK 284
IF T++ G +VPY + DV+ ++ G + + + I+ + G+L D+
Sbjct: 263 LCGGIIFGTVA---GFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDR 319

Query: 285 VGS--AIKVMNWLFIAGVIGVAMFLVIPQDPAMVWVLIGTLMIVGSINF 331
G + + + + L + ++ I + ++G ++F
Sbjct: 320 RGPLYVLNIGVTFLSVSFLTASFLL----ETTSWFMTIIIVFVLGGLSF 364


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3443TCRTETA310.008 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 30.9 bits (70), Expect = 0.008
Identities = 59/306 (19%), Positives = 110/306 (35%), Gaps = 34/306 (11%)

Query: 42 NEYFSLTNTQS--GMLMSWLGFVGIISGAVSGIIVDRFKNPKSILTIAYLTMAALAIWQS 99
+ + + G+L++ + V G + DRF + +L ++ A +
Sbjct: 33 RDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGR-RPVLLVSLAGAAVDYAIMA 91

Query: 100 FRPSYQAMFI--IVGFMSLVGNGLFLVSMTKIARLLASDNEQGRYFGFLESGRGIAGTVL 157
P ++I IV ++ V+ IA + D E+ R+FGF+ + G
Sbjct: 92 TAPFLWVLYIGRIVAGIT---GATGAVAGAYIADITDGD-ERARHFGFMSACFGFG---- 143

Query: 158 TLCAVAIVGLHGSSAVSIGFILRFDAAIYIIPGFTSYYLFPKGVSAIENAA------PKK 211
+ + GL G + F AA+ + T +L P+ P
Sbjct: 144 MVAGPVLGGLMGGFSPHAPFF--AAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLA 201

Query: 212 MSDLISLLKSVKLWLAAFIISCVIFVYQGGAYL-VPYLSDAYGMTPDQT----AVIGMIR 266
+ V +A F I + V Q A L V + D + A G++
Sbjct: 202 SFRWARGMTVVAALMAVFFI--MQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILH 259

Query: 267 AYFLAFIISPFAGLLADK--IGSSLKVMASFFILGALITASFIFIPHDSRFLILLITLVL 324
+ A I P A L ++ + + + +IL A T ++ P ++LL + +
Sbjct: 260 SLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFP----IMVLLASGGI 315

Query: 325 LLGALT 330
+ AL
Sbjct: 316 GMPALQ 321


96SPA3550SPA3557N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3550-213-0.935057hypothetical protein
SPA35510191.307741serine acetyltransferase
SPA35521162.179702glycerol-3-phosphate dehydrogenase
SPA35531171.888396protein-export protein SecB
SPA35540120.936258glutaredoxin 3
SPA3555-1120.931914hypothetical protein
SPA35560131.5244302,3-bisphosphoglycerate-independent
SPA35570140.831382hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3550TCRTETA401e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 40.2 bits (94), Expect = 1e-05
Identities = 68/410 (16%), Positives = 129/410 (31%), Gaps = 65/410 (15%)

Query: 35 VAPIMSKELGFDPEA---MGLAFSSFGIAYVIMQLPGGWLLDRYGSRLVYGCALIGWSLV 91
V P + ++L + G+ + + + G L DR+G R V +L G ++
Sbjct: 27 VLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAV- 85

Query: 92 TMFQGTIYLYGSPLIVLVILRLLMGAIEAPAFPANSRLS--------VQWFPNNERGFVT 143
I L VL I R++ G A A + ++ + F GF++
Sbjct: 86 ---DYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHF-----GFMS 137

Query: 144 SVYQAAQYISLGIITPLMTIILHNLSWHFVFYYIGAIGV---MLGIFWLMKVKDPMHHPK 200
+ + + P++ ++ S H F+ A+ + G F L + P
Sbjct: 138 ACFGFGM-----VAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPL 192

Query: 201 VNQAEIDYIRSGGGEPSLGCKKEPQKITFAQIKTVCVNRMMIGVYIGQFCVTSITWFFLT 260
+ A + ++ + F + +
Sbjct: 193 ---------------------RREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAA 231

Query: 261 WFPTYLYQAKGMSILKVGFVASIPAIAGFIGGLLGGVFSDWLLKRGYSLTVARKLPVICG 320
+ + +G A G + L + + + R ++ G
Sbjct: 232 LWVIFGEDRFHWDATTIGISL---AAFGILHSLAQAMITGPVAARLGERRA-----LMLG 283

Query: 321 MLLSCV--IVIANYTSSEFVVIAAMSLAFFAKGFGNLGWCVLSDTSPKEVLGIAGGVFNM 378
M+ I++A T + LA G L +LS +E G G
Sbjct: 284 MIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQ-AMLSRQVDEERQGQLQGSLAA 342

Query: 379 CGNMASIVTPLVIGVILANTQSFDFAILYVGSMGLIGLISYLFIVGPLDR 428
++ SIV PL+ I A + + + G + G YL + L R
Sbjct: 343 LTSLTSIVGPLLFTAIYAASITT-----WNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3552NUCEPIMERASE290.028 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 29.0 bits (65), Expect = 0.028
Identities = 21/87 (24%), Positives = 30/87 (34%), Gaps = 13/87 (14%)

Query: 8 MTVI---GAGSYGTALAITLARNGHQVVLWGHD---PKHIATLEHDRCNVAFLPDVPFPD 61
M + AG G ++ L GHQVV G D + +L+ R + P F
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVV--GIDNLNDYYDVSLKQARLELLAQPGFQF-- 56

Query: 62 TLHLESDLATALAASRNILVVVPSHVF 88
+ DLA + VF
Sbjct: 57 ---HKIDLADREGMTDLFASGHFERVF 80


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3553SECBCHAPRONE2342e-82 Bacterial protein-transport SecB chaperone protein ...
		>SECBCHAPRONE#Bacterial protein-transport SecB chaperone protein

signature.
Length = 170

Score = 234 bits (598), Expect = 2e-82
Identities = 91/153 (59%), Positives = 118/153 (77%), Gaps = 4/153 (2%)

Query: 3 EQNNTEMAFQIQRIYTKDVSFEAPNAPHVFQKDWQPEVKLDLDTASSQLADDVYEVVLRV 62
Q + QIQRIY KDVSFEAPN PH+FQ+DW+P++ DL T + Q+ DD+YEV L +
Sbjct: 12 TQATQQPVLQIQRIYVKDVSFEAPNLPHIFQQDWEPKLSFDLSTEAKQVGDDLYEVCLNI 71

Query: 63 TVTASLGEE--TAFLCEVQQAGIFSISGIEGTQMAHCLGAYCPNILFPYARECITSLVSR 120
+V ++ AF+CEV+QAG+F+ISG+E QMAHCL + CPN+LFPYARE ++SLV+R
Sbjct: 72 SVETTMESSGDVAFICEVKQAGVFTISGLEEMQMAHCLTSQCPNMLFPYARELVSSLVNR 131

Query: 121 GTFPQLNLAPVNFDALFMNYL--QQQAGEGTEE 151
GTFP LNL+PVNFDALFM+YL Q+QA + TEE
Sbjct: 132 GTFPALNLSPVNFDALFMDYLQRQEQAEQTTEE 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3557RTXTOXIND477e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.1 bits (112), Expect = 7e-08
Identities = 25/196 (12%), Positives = 62/196 (31%), Gaps = 21/196 (10%)

Query: 45 RDQLKSIQADIAAKERDVRQQQQQRASLLAQLKAQEEAISAAARKLRETQSTLDQLNAQI 104
++ + + Q +L +E S + + + LD+ A+
Sbjct: 157 SRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAER 216

Query: 105 DEMNASIAKLEQQKASQERNLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLN 164
+ A I + E ++ L + + ++ Q + ++A
Sbjct: 217 LTVLARINRYENLSRVEKSRLDD-FSSLLHKQAIAKHAVL-----EQENKYVEA-----V 265

Query: 165 QARQETIAELKQTREQVATQKAELEEKQSQQQTLLYEQRAQ-QAKLEQARNERKKTLAGL 223
+ ++L+Q ++ + K E + + + ++ Q + E K
Sbjct: 266 NELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEE-- 323

Query: 224 ESSIQQGQQQLSELRA 239
+QQ S +RA
Sbjct: 324 -------RQQASVIRA 332


97SPA3604SPA3612N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3604319-4.462059hypothetical protein
SPA3606319-3.796576DNA-binding protein
SPA3607419-3.732464hypothetical protein
SPA3608213-1.799967autotransported protein
SPA3609-116-2.609124hypothetical protein
SPA3610-314-1.715501transcriptional regulator
SPA3611-217-1.332602hypothetical protein
SPA3612-216-0.481999inner membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3604IGASERPTASE352e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.7 bits (79), Expect = 2e-04
Identities = 27/108 (25%), Positives = 47/108 (43%), Gaps = 3/108 (2%)

Query: 25 GYHIEHVENKSQQPGRTFDYQNLAASALDSENGLPQLGINAFGGHVQG-KNKSVDMAQFI 83
GY + S + +F+ NL + +E+ LG G +Q N V + +
Sbjct: 793 GYVTCTTDKLSDKALNSFNPTNLRGNVNLTESANFVLGKANLFGTIQSRGNSQVRLTENS 852

Query: 84 HCHLP-DCSRYFAYLSNGHV-VPSIDLTEQEAEYAQYTIDHLNLNSGF 129
H HL + + L+NGH+ + S D + +Y T++ L+ N F
Sbjct: 853 HWHLTGNSDVHQLDLANGHIHLNSADNSNNVTKYNTLTVNSLSGNGSF 900


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3608PERTACTIN1191e-29 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 119 bits (300), Expect = 1e-29
Identities = 164/749 (21%), Positives = 289/749 (38%), Gaps = 90/749 (12%)

Query: 230 TGDSSEGLRTGQSGSLIRLGDDATIETSGASSTGIYAASSSRTELGNNATITVNGASAHA 289
TG + G+ G+++ L ATI A + G + +
Sbjct: 236 TGGRAAGV-AAMDGAIVHL-QRATIRRGDAPAGGAVPGGAVPGGAVPGG-FGPLLDGWYG 292

Query: 290 VYATNATVNLGENATISVNSASKAASYSKAPAGLYALSRGAINLAGGAAITMAGDNSSES 349
V +++TV+L A V + A+ +S G+++ G I G
Sbjct: 293 VDVSDSTVDL---AQSIVEAPQLGAAIRAGRGARVTVSGGSLSAPHGNVIETGGGARRFP 349

Query: 350 YAISTETGGIVDGS--SGGRFVIDGDIRAAGATAASGTLPQ--------------QNSTI 393
S + + G+ G + T A G Q + +
Sbjct: 350 PPASPLSITLQAGARAQGRALLYRVLPEPVKLTLAGGAQGQGDIVATELPPIPGASSGPL 409

Query: 394 KLNMTDNSRWDGASYITSATAGTGVISVQMSDATWNMTSSSTLTDLTLNSGATINFSH-- 451
+ + +RW GA+ V S+ + +ATW MT +S + L L S +++F
Sbjct: 410 DVALASQARWTGATRA--------VDSLSIDNATWVMTDNSNVGALRLASDGSVDFQQPA 461

Query: 452 EDGEPWQTLTINEDYVGNGGKLVFNTVLSDDDSETDRLQVLGNTSGNTFVAVNNIGGAGA 511
E G ++ L ++ G+G +F + D +D+L V+ + SG + V N G A
Sbjct: 462 EAGR-FKVLMVDT-LAGSG---LFRMNVFADLGLSDKLVVMRDASGQHRLWVRNSGSEPA 516

Query: 512 QTIEGIEIVNVAGNSNGTFEKASR---IVAGAYDYNMVQKGKNWYLTSYIEPDEPIIPDP 568
+ + +V S TF A++ + G Y Y + G + S + P P P
Sbjct: 517 -SGNTMLLVQTPRGSAATFTLANKDGKVDIGTYRYRLAANGNGQW--SLVGAKAPPAPKP 573

Query: 569 VDPVIPDPVIPDPVDPDPVDPVIPDPVIPDPVDPEPVDPVIPDPTIPDIGQSDTPPITEH 628
P P P P P P P P P +P P P ++ + +
Sbjct: 574 APQPGPQPGPQPPQPPQPPQP----PQPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTG 629

Query: 629 QFRPEVGSYLANNYAANTLFMTRLHDRLGETQYTDMLTGEKKVTSLWMRNVGAHTRFNDG 688
+ A + A L RLGE + G W R + ++
Sbjct: 630 GVGLASTLWYAESNA--------LSKRLGELRLNPDAGG------AWGRGFAQRQQLDNR 675

Query: 689 SGQLKTRINSYVLQLGGDLAQWSTDGLDRWHIGAMAGYANSQNRTQSSVSDYHSRGQVTG 748
+G+ + +LG D A + G RWH+G +AGY + D G
Sbjct: 676 AGRRFDQ-KVAGFELGADHA-VAVAG-GRWHLGGLAGYTRGD---RGFTGD--GGGHTDS 727

Query: 749 YSVGLYGTWYANNIDRSGAYVDTWMLFNWFDN--KVMGQDQAA--EKYKSKGITASVEAG 804
VG Y T+ AN+ G Y+D + + +N KV G D A KY++ G+ S+EAG
Sbjct: 728 VHVGGYATYIANS----GFYLDATLRASRLENDFKVAGSDGYAVKGKYRTHGVGVSLEAG 783

Query: 805 YSFRLGESVHQSYWLQPKAQVVWMGVQADDNREANGTLVKDDTAGNLLTRMGVKAYINGH 864
F ++L+P+A++ V R ANG V+D+ ++L R+G++
Sbjct: 784 RRFAH----ADGWFLEPQAELAVFRVGGGAYRAANGLRVRDEGGSSVLGRLGLEV----G 835

Query: 865 NAIDDNKSREFQPFVEANWIHNTQPA-SVKMDDVS--SDMRGTKNIGELKVGIEGQITPR 921
I+ R+ QP+++A+ + A +V+ + ++ +++RGT+ EL +G+ +
Sbjct: 836 KRIELAGGRQVQPYIKASVLQEFDGAGTVRTNGIAHRTELRGTR--AELGLGMAAALGRG 893

Query: 922 LNVWGNVAQQVGDQGYSNTQGLLGVKYSF 950
+++ + G + G +YS+
Sbjct: 894 HSLYASYEYSKGPKLAMPWTFHAGYRYSW 922


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3611ISCHRISMTASE426e-07 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 42.3 bits (99), Expect = 6e-07
Identities = 43/180 (23%), Positives = 63/180 (35%), Gaps = 22/180 (12%)

Query: 1 MSTPANF--NGQRPAIDANDAVMLLIDHQSGLFQTVGD--MPMPELRACAAALAKIATLC 56
M T ++ N D N AV+L+ D Q+ P+ EL A L
Sbjct: 11 MPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQL 70

Query: 57 NMPVITTASVPQ-------------GPNGPLIPE----IHANAPHA-QYVARKGEINAWD 98
+PV+ TA GP P I AP V K +A+
Sbjct: 71 GIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFK 130

Query: 99 NADFVQAVKATGRKTLIIAGTITSVCMAFPAISAVAEGYKVFAVIDASGTYSKMAQEITM 158
+ ++ ++ GR LII G + A A E K F V DA +S ++ +
Sbjct: 131 RTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFSLEKHQMAL 190


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3612cloacin290.009 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 28.9 bits (64), Expect = 0.009
Identities = 13/47 (27%), Positives = 21/47 (44%)

Query: 30 NGNGGGHGNNAANQGNNGNGHKGNAGQKTEHRKNGGKPDHVESDISY 76
N GGG G+ G +G+G+ G G GG V + +++
Sbjct: 44 NPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAF 90


98SPA3671SPA3677N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3671-2130.828235TorD protein
SPA3672-2121.225842trimethylamine-N-oxide reductase precursor
SPA3673-2120.899385hypothetical protein
SPA3674-1131.489018response regulator in multi-component regualtory
SPA3675-2101.536963Solute binding receptor protein
SPA3676-1111.589301two-component sensor protein histidine protein
SPA3677-1172.180093MFS family, D-galactonate transport protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3671PF06872290.021 EspG protein
		>PF06872#EspG protein

Length = 398

Score = 28.5 bits (63), Expect = 0.021
Identities = 14/54 (25%), Positives = 27/54 (50%)

Query: 111 LLLEAGMEVNDDFKEPTDHLAIYLELLSHLHFSLGESFQQRRMNKLRQKTLSSL 164
L+L+A +++N D+K+P + + +LL L L + + Q L+ L
Sbjct: 29 LVLDATIKINSDYKKPWNEMTCAEKLLKILTLGLWNPKYSQDERQQFQGLLTVL 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3674HTHFIS762e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.4 bits (188), Expect = 2e-18
Identities = 28/115 (24%), Positives = 56/115 (48%), Gaps = 1/115 (0%)

Query: 4 HIVIVEDEPVTQARLQAYFEQEGYRVSVTDSGAGLRDIMEHEHVSLILLDINLPDENGLM 63
I++ +D+ + L + GY V +T + A L + L++ D+ +PDEN
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 LTRALRER-STVGIILVTGRCDQIDRIVGLEMGADDYVTKPLELRELVVRVKNLL 117
L +++ + +++++ + + I E GA DY+ KP +L EL+ + L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3676HTHFIS559e-10 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 54.8 bits (132), Expect = 9e-10
Identities = 24/118 (20%), Positives = 49/118 (41%), Gaps = 3/118 (2%)

Query: 681 RLLLIEDNMLTQRITAEMLTGKGVKVSVAESANDALRCLAEGESFDVALVDFDLPDYDGL 740
+L+ +D+ + + + L+ G V + +A R +A G+ D+ + D +PD +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD-GDLVVTDVVMPDENAF 63

Query: 741 TLAQQLMSLYPAMKRIGFSAH-VIDDNLRQRTAGLFCGIIQKPVPREELYRMIAHYLQ 797
L ++ P + + SA ++ G + + KP EL +I L
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAY-DYLPKPFDLTELIGIIGRALA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3677TCRTETA462e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 45.6 bits (108), Expect = 2e-07
Identities = 65/384 (16%), Positives = 117/384 (30%), Gaps = 36/384 (9%)

Query: 66 AEMGYVFSAFAWLYTLCQIPGGWFLDRIGSRLTYFIAIFGWSVATLLQGFATGLLSLIGL 125
A G + + +A + C G DR G R +++ G +V + A L L
Sbjct: 43 AHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG 102

Query: 126 RAITGIFEAPAFPANNRMVTSWFPEHERASAVGFYTSGQFVGLAFLTPLLIWIQEMLSWH 185
R + GI A + ERA GF ++ G+ P+L + S H
Sbjct: 103 RIVAGITGAT-GAVAGAYIADITDGDERARHFGFMSACFGFGMV-AGPVLGGLMGGFSPH 160

Query: 186 WVFIVTGGIGIIWSLIWFKVYQPPRLTKSLSQAELEYIRDGGGLVDGDAPAKKEARQPLT 245
F + + L + + P ++EA PL
Sbjct: 161 APFFAAAALNGLNFLTGCFLLPESHKGE-------------------RRPLRREALNPLA 201

Query: 246 KADWKLVFHRKLVGVYLGQFAVNSTLWFFLTWFPNYLTQEKGITALKAGFMTTV-PFLAA 304
W + + F + + + A G L +
Sbjct: 202 SFRWARGM-TVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHS 260

Query: 305 FFGVLLSGWLADKLVKKGFSLGVARKTPIICGLLISTC--IMGANYTNDPFWIMALMAIA 362
+++G +A +L + ++ G++ I+ A T ++ +A
Sbjct: 261 LAQAMITGPVAARL---------GERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLA 311

Query: 363 FFGNGFASITWSLISSLAPMRLIGLTGGMFNFIGGLGGISVPLVIGYL-AQSYGFAPALV 421
G G ++ +++S G G + L I PL+ + A S
Sbjct: 312 SGGIGMPALQ-AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWA 370

Query: 422 YISVVALLGALSYILLVGDVKRVG 445
+I+ AL L G G
Sbjct: 371 WIAGAALYLLCLPALRRGLWSGAG 394


99SPA3844SPA3850N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA3844-2131.059302hypothetical protein
SPA38450181.094432oxygen-independent coproporphyrinogen III
SPA38460170.816964two-component system, response regulator
SPA3847016-0.437103two-component system sensory histidine kinase
SPA3848014-2.073333glutamine synthetase
SPA3849-114-3.997706hypothetical protein
SPA3850-213-3.986960GTP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3844SECA280.015 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 28.3 bits (63), Expect = 0.015
Identities = 14/74 (18%), Positives = 27/74 (36%)

Query: 11 KAFGKQRRKTREELNQEARDRKRLKKHRGHAPGSRAAGGNSASGGGNQNQQKDPRIGSKT 70
K + + EE+ + + R+ + +SA+ Q + ++G
Sbjct: 824 STLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRND 883

Query: 71 PVPLGVTEKVTQQH 84
P P G +K Q H
Sbjct: 884 PCPCGSGKKYKQCH 897


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3846HTHFIS5970.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 597 bits (1540), Expect = 0.0
Identities = 204/478 (42%), Positives = 299/478 (62%), Gaps = 11/478 (2%)

Query: 1 MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGNEVLAALASKTPDVLLSDIRMPGM 60
M + V DDD++IR VL +AL+ AG N + +A+ D++++D+ MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS 120
+ LL +IK+ P LPV++M+A + A+ A ++GA+DYLPKPFD+ E + ++ RA++
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 HYQEQQQPRNIEVNGPTTDMIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA 180
+ + + ++G + AMQ+++R++ RL ++ ++++I GESGTGKELVA A
Sbjct: 121 EPKRRPSKLEDDSQDGM-PLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARA 179

Query: 181 LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE 240
LH + R PF+A+NMAAIP+DLIESELFGHEKGAFTGA T GRFEQA+GGTLFLDE
Sbjct: 180 LHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDE 239

Query: 241 IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLERRVQEGKFREDLFHR 300
IGDMP+D QTRLLRVL G++ VGG P++ DVRI+AAT+++L++ + +G FREDL++R
Sbjct: 240 IGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYR 299

Query: 301 LNVIRIHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETETALTRLAWPGNVRQL 360
LNV+ + LPPLR+R EDIP L RHF+Q A +E G++ K E + WPGNVR+L
Sbjct: 300 LNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALELMKAHPWPGNVREL 358

Query: 361 ENTCRWLTVMAAGQEVLTQDLPGELFEASTPDSPSHLPPDSWATLLAQWADRALRS---- 416
EN R LT + + + + EL S + ++Q + +R
Sbjct: 359 ENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFAS 418

Query: 417 -----GHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME 469
L E+E L+ AL T+G++ +AA LLG RNTL +K++ELG+
Sbjct: 419 FGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3847PF06580290.034 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 28.7 bits (64), Expect = 0.034
Identities = 33/189 (17%), Positives = 71/189 (37%), Gaps = 39/189 (20%)

Query: 171 IIEQADRLRNLVDRL-------LGPQHPGMHIT--ESIHKVAERVVALVSMELPDNVRLI 221
I+E + R ++ L L ++ + + V + + L S++ D ++
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYS-NARQVSLADELTVV-DSYLQLASIQFEDRLQFE 243

Query: 222 RDYDPSLPELPHDPEQIEQVLL-NIVRNALQALGPEGGEITLRTRTAFQLTLHGERYRLA 280
+P++ ++ P + Q L+ N +++ + L P+GG+I L+
Sbjct: 244 NQINPAIMDVQV-PPMLVQTLVENGIKHGIAQL-PQGGKILLKGT------KDNGTVT-- 293

Query: 281 ARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHAGK---IEFTSWPG 337
++VE+ G + ++ TG GL R + G I+ + G
Sbjct: 294 --LEVENTGSLALKNTKE------------STGTGLQNVRERLQMLYGTEAQIKLSEKQG 339

Query: 338 HTEFSVYLP 346
V +P
Sbjct: 340 KVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA3850TCRTETOQM1797e-51 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 179 bits (456), Expect = 7e-51
Identities = 100/448 (22%), Positives = 170/448 (37%), Gaps = 87/448 (19%)

Query: 4 NLRNIAIIAHVDHGKTTLVDKLLQQSGTFDARAETQE--RVMDSNDLEKERGITILAKNT 61
+ NI ++AHVD GKTTL + LL SG + D+ LE++RGITI T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 62 AIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAFDGPMPQTRFVTKKAFAHGL 121
+ +W + ++NI+DTPGH DF EV R +S++D +L++ A DG QTR + G+
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 122 KPIVVINKVDRPGARPDWVVDQVFD-------------LFVNLDATDEQLD--------- 159
I INK+D+ G V + + L+ N+ T+
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 160 --------------------------------FPIIYASALNGIAGLDHEDMAEDMTPLY 187
FP+ + SA N I G+D+ L
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNI-GIDN---------LI 231

Query: 188 QAIVDHVPAPDVDLDGPLQMQISQLDYNNYVGVIGIGRIKRGKVKPNQQVTIIDSEGKTR 247
+ I + + L ++ +++Y+ + R+ G + V I + E
Sbjct: 232 EVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-- 289

Query: 248 NAKVGKVLTHLGLERIDSDIAEAGDIIAITGLG-ELN--ISDTICDPQNVEALPALSVDE 304
K+ ++ T + E D A +G+I+ + +LN + DT PQ +
Sbjct: 290 --KITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQR----ERIENPL 343

Query: 305 PTVSMFFCVNTSPFCGKEGKFVTSRQILDRLNKELVHNVALRVEETEDADAFRVSGRGEL 364
P + + + D L LR +S G++
Sbjct: 344 PLLQTTVEPSKPQQREMLLDALLEISDSDPL---------LRYYVDSATHEIILSFLGKV 394

Query: 365 HLSVLIENMRRE-GFELAVSRPKVIFRE 391
+ V ++ + E+ + P VI+ E
Sbjct: 395 QMEVTCALLQEKYHVEIEIKEPTVIYME 422



Score = 32.5 bits (74), Expect = 0.005
Identities = 13/75 (17%), Positives = 29/75 (38%), Gaps = 1/75 (1%)

Query: 398 EPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIGFRSEFMT 457
EPY + + +++ + ++ + V L IP+R + +RS+
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKN-NEVILSGEIPARCIQEYRSDLTF 595

Query: 458 MTSGTGLLYSTFSHY 472
T+G + + Y
Sbjct: 596 FTNGRSVCLTELKGY 610


100SPA4007SPA4022N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4007-1152.873736histone like DNA-binding protein HU-alpha (NS2)
SPA4008-1173.530766hypothetical protein
SPA4009-2173.116450hypothetical protein
SPA4010-1152.145278two-component system sensor protein
SPA4011-1151.459161transcriptional regulator
SPA4012-1191.592110phosphoribosylglycineamide synthetase
SPA4013-1160.646779bifunctional
SPA4019-1120.304926*acetyltransferase
SPA4020-1172.143587homoserine O-succinyltransferase
SPA4021-1182.908684malate synthase A
SPA4022-1213.847271isocitrate lyase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4007DNABINDINGHU1201e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 120 bits (303), Expect = 1e-39
Identities = 49/89 (55%), Positives = 66/89 (74%)

Query: 2 NKTQLIDVIADKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNHRAERTGR 61
NK LI +A+ EL+K + AA+++ +A++ L +G+ VQL+GFG F+V RA R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEIKIAAANVPAFVSGKALKDAVK 90
NPQTG+EIKI A+ VPAF +GKALKDAVK
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAVK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4010PF06580362e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 36.4 bits (84), Expect = 2e-04
Identities = 25/132 (18%), Positives = 49/132 (37%), Gaps = 29/132 (21%)

Query: 340 QLRFTANETLK-RIQADPDRLTQVLLNLYL-----NAI-HAIGRQ---GTISVEAKESGT 389
++F + L+ Q +P + + + + N I H I + G I ++ +
Sbjct: 233 SIQF--EDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDN- 289

Query: 390 DRVIITVTDSGKGIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGG---AIKVKSI 446
V + V ++G + E TG GL V+ ++ G IK+
Sbjct: 290 GTVTLEVENTGSLALKNTKE------------STGTGLQNVRERLQMLYGTEAQIKLSEK 337

Query: 447 EGKGAVFTIWLP 458
+GK + +P
Sbjct: 338 QGKVNA-MVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4011HTHFIS5180.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 518 bits (1336), Expect = 0.0
Identities = 180/475 (37%), Positives = 255/475 (53%), Gaps = 37/475 (7%)

Query: 1 MIRGKIDILVVDDDVSHCTILQALLRGWGYNVALAYSGHDALAQVREKVFDLVLCDVRMA 60
M I LV DDD + T+L L GY+V + + + DLV+ DV M
Sbjct: 1 MTGATI--LVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 EMDGIATLKEIKALNPAIPILIMTAFSSVETAVEALKAGALDYLIKPLDFDRLQETLEKA 120
+ + L IK P +P+L+M+A ++ TA++A + GA DYL KP D L + +A
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 121 LAHTRETGAELPSASAAQFGMIGSSPAMQHLLNEIAMVAPSDATVLIHGDSGTGKELVAR 180
LA + ++L S ++G S AMQ + +A + +D T++I G+SGTGKELVAR
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVAR 178

Query: 181 ALHACSARSDKPLVTLNGAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD 240
ALH R + P V +N AA+ L+ESELFGHEKGAFTGA R GRF +A+GGTLFLD
Sbjct: 179 ALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLD 238

Query: 241 EISDISPLMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAEEVSAGRFRQDLYY 300
EI D+ Q RLLR +Q+ E VG I DVR++AAT++DL + ++ G FR+DLYY
Sbjct: 239 EIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYY 298

Query: 301 RLNVVAIEMPSLRQRREDIPLLADHFLRRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE 360
RLNVV + +P LR R EDIP L HF+++ + VK F +A++L+ + WPGN+RE
Sbjct: 299 RLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLD-VKRFDQEALELMKAHPWPGNVRE 357

Query: 361 LENAIERAVVLLTGEYISERELPLAIAATPIKTEYSGEIQP------------------- 401
LEN + R L + I+ + + + +
Sbjct: 358 LENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFA 417

Query: 402 ---------------LVDVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR 441
L ++E +ILAAL T GN+ +AA LG+ R TL K+
Sbjct: 418 SFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4019SACTRNSFRASE392e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.8 bits (90), Expect = 2e-06
Identities = 16/54 (29%), Positives = 22/54 (40%), Gaps = 5/54 (9%)

Query: 78 VDPDVRGQGIGKRLVEHALTLAP-----GLTTNVNEQNTQAVGFYKKMGFKVTG 126
V D R +G+G L+ A+ A GL + N A FY K F +
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4022BINARYTOXINB320.008 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 31.6 bits (71), Expect = 0.008
Identities = 14/58 (24%), Positives = 23/58 (39%)

Query: 289 ETSTPDLELARRFADAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIASFQQQLSDMGYK 346
ET+ PD+ L A P L Y + N D +T + + QL+++
Sbjct: 544 ETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNAT 601


101SPA4105SPA4110N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4105-112-0.225606acetyltransferase
SPA4106-112-0.847909hypothetical protein
SPA4107-113-0.215081hypothetical protein
SPA4108-211-1.079656ProP
SPA4109-112-0.614789two-component sensor kinase
SPA4110-112-1.050936two-component response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4105SACTRNSFRASE332e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 33.4 bits (76), Expect = 2e-04
Identities = 20/86 (23%), Positives = 33/86 (38%), Gaps = 9/86 (10%)

Query: 58 LALRNGEVVGMISLHMQFHLHHANWIG--EIQELVVLPPMRGQKIGSQLLAWAEEEARQA 115
L +G I + +NW G I+++ V R + +G+ LL A E A++
Sbjct: 69 LYYLENNCIGRIKIR-------SNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKEN 121

Query: 116 GAELTELSTNIKRRDAHRFYLREGYK 141
L T A FY + +
Sbjct: 122 HFCGLMLETQDINISACHFYAKHHFI 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4108TCRTETA449e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 44.0 bits (104), Expect = 9e-07
Identities = 53/284 (18%), Positives = 104/284 (36%), Gaps = 40/284 (14%)

Query: 85 FFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYATIGIWAPILLLLCKMAQGFSIGGE 144
G L D++GR+ +L +++ ++ + P +W +L + ++ G + G
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPF-----LW---VLYIGRIVAGIT-GAT 112

Query: 145 YTGASIFVAEYSPDRKR----GFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLEWGW 200
A ++A+ + +R GFM + FG +AG VLG G++ S
Sbjct: 113 GAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG-GLMGGFSP------------ 159

Query: 201 RIPFFIALLLGIIGLYLRHALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRS 260
PFF A L + L K E+ P SF+ +
Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPESH------KGERRPLRREALNPLASFRWARGMTVVA 213

Query: 261 LLSCIGLVIATNVTYYMLLTYMPSYLSHNLHYS-EDHGVLIIIAIMIGMLFVQPVMGLLS 319
L + ++ + + + H+ G+ + ++ L + G ++
Sbjct: 214 ALMAVFFIM--QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVA 271

Query: 320 DRFGRRPFVIMGSIA-LFALAIPAFILINSNVIGLIFAGLLMLA 362
R G R +++G IA + AF + F +++LA
Sbjct: 272 ARLGERRALMLGMIADGTGYILLAFA----TRGWMAFPIMVLLA 311



Score = 37.9 bits (88), Expect = 8e-05
Identities = 37/164 (22%), Positives = 73/164 (44%), Gaps = 16/164 (9%)

Query: 286 LSHNLHYSEDHGVLI-IIAIMIGMLFVQPVMGLLSDRFGRRPFVIMGSIALFALAIPAFI 344
L H+ + +G+L+ + A+M PV+G LSDRFGRRP ++ ++L A+ I
Sbjct: 35 LVHSNDVTAHYGILLALYALM--QFACAPVLGALSDRFGRRPVLL---VSLAGAAVDYAI 89

Query: 345 LINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIR---YSALAAAFNISVLIAG 401
+ + + +++ G ++A I V + + + R + ++A F ++AG
Sbjct: 90 MATAPFLWVLYIG-RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFG-MVAG 147

Query: 402 LTPTLAAWLVESSQDLMMPAYYLMVIAVIGLVTGI-SMKETANR 444
P L + S P + + + +TG + E+
Sbjct: 148 --PVLGGLMGGFS--PHAPFFAAAALNGLNFLTGCFLLPESHKG 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4109PF06580385e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 37.5 bits (87), Expect = 5e-05
Identities = 39/182 (21%), Positives = 78/182 (42%), Gaps = 34/182 (18%)

Query: 181 ARLDQMMDSVSQLLQLARVGQSFSSGNYQEVKLLEDV-ILPSYDELNTM-LETR-QQTLL 237
+ +M+ S+S+L++ S N ++V L +++ ++ SY +L ++ E R Q
Sbjct: 191 TKAREMLTSLSELMR-----YSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQ 245

Query: 238 LPESAADVVVRGDATLLRMLLRNLVENAHRY----SPEGTHITIHISADPDAI-MAVEDE 292
+ + DV V ML++ LVEN ++ P+G I + + D + + VE+
Sbjct: 246 INPAIMDVQV------PPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENT 299

Query: 293 GPGIDESKCGKLSEAFVRMDSRYGGIGLGLSIV-SRITQLHQGQFFLQNRTERTGTRAWV 351
G + + G GL V R+ L+ + ++ ++ A V
Sbjct: 300 GSLA--------------LKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMV 345

Query: 352 LL 353
L+
Sbjct: 346 LI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4110HTHFIS928e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 92.2 bits (229), Expect = 8e-24
Identities = 46/144 (31%), Positives = 69/144 (47%), Gaps = 1/144 (0%)

Query: 2 KILIVEDDTLLLQGLILAAQTEGYACDGVSTARAAEHSLESGHYSLMVLDLGLPDEDGLH 61
IL+ +DD + L A GY S A + +G L+V D+ +PDE+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 FLTRIRQKKYTLPVLILTARDTLNDRISGLDVGADDYLVKPFALEELHARI-RALLRRHN 120
L RI++ + LPVL+++A++T I + GA DYL KPF L EL I RAL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 121 NQGESELTVGNLTLNIGRHQAWRD 144
+ E + +GR A ++
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQE 148


102SPA4175SPA4181N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA4175-2132.923577N-acetylmuramoyl-L-alanine amidase
SPA41760162.273677DNA mismatch repair protein
SPA41771181.204986tRNA delta-2-isopentenylpyrophosphate (IPP)
SPA41784231.028415host factor-I protein
SPA41794210.888767HflX protein, putative GTP-binding protein
SPA41803201.172013HflK protein
SPA41814180.990706HflC protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4175PF03544310.007 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 31.1 bits (70), Expect = 0.007
Identities = 16/65 (24%), Positives = 26/65 (40%), Gaps = 7/65 (10%)

Query: 130 PPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASAGAGDKVVI 189
P P P P K+VE R +P + S + + RP + + A K V
Sbjct: 99 PKPKPKPKPVKKVEQPKRDVKPVESRPASPFE-------NTAPARPTSSTATAATSKPVT 151

Query: 190 AIDAG 194
++ +G
Sbjct: 152 SVASG 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4176ALARACEMASE300.027 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 30.1 bits (68), Expect = 0.027
Identities = 26/161 (16%), Positives = 57/161 (35%), Gaps = 18/161 (11%)

Query: 31 VENSLDAGATRVDIDIER---GGAKLIR-IRDNGCGIKKEELALALARHATSKIASLDDL 86
++ SLD A + ++ I R A++ ++ N G E + A+ + +L++
Sbjct: 5 IQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLNLEEA 64

Query: 87 EAIISLGFRGEAL----------ASISSVSRLTLTSRTAEQAEAWQAYAEGRDMDVTVK- 135
+ G++G L I RLT + Q +A Q +D+ +K
Sbjct: 65 ITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKV 124

Query: 136 -PAAHPVGTTLEVLDLFYNTPARRKFMRTEK--TEFNHIDE 173
+ +G + + + + + F +
Sbjct: 125 NSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEH 165


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4179SECA330.002 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 33.3 bits (76), Expect = 0.002
Identities = 26/144 (18%), Positives = 55/144 (38%), Gaps = 6/144 (4%)

Query: 282 HVVDAADVRVQENIEAVNTVLEEIDAHEIPTLMVMNKIDMLDDFEPRIDRDEENK-PIRV 340
++D +DV N + IDA+ P + ++ + + R+ D + PI
Sbjct: 665 ELLDVSDVSETINSIREDVFKATIDAYIPPQSL--EEMWDIPGLQERLKNDFDLDLPIAE 722

Query: 341 WLSAQSGVGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKEWMEEDGSV 400
WL + + L + + + + + + R + LQ ++ W E ++
Sbjct: 723 WLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAM 782

Query: 401 SLQVRMPIVDWRRLCKQEPALIEY 424
+R I R +++P EY
Sbjct: 783 D-YLRQGIH-LRGYAQKDP-KQEY 803


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4181PYOCINKILLER290.030 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 29.0 bits (64), Expect = 0.030
Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 3/65 (4%)

Query: 225 NRMRAEREAVARRHRSQGQEEAEKLRAAADYEVTK---TLAEAERQGRIMRGEGDAEAAK 281
N+ R + A A+R + + +RAA Y + +A A +G I +G A A+
Sbjct: 220 NKAREQAAAEAKRKAEEQARQQAAIRAANTYAMPANGSVVATAAGRGLIQVAQGAASLAQ 279

Query: 282 LFADA 286
+DA
Sbjct: 280 AISDA 284


103SPA4372SPA4378N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SPA43720130.907686ribosomal-protein-alanine acetyltransferase
SPA4373-1131.752440hypothetical protein
SPA4374-2142.206160peptide chain release factor 3
SPA4375-1112.041603hypothetical protein
SPA4376-1112.542895hypothetical protein
SPA4377-1122.605578hypothetical protein
SPA4378-2162.698139hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4372SACTRNSFRASE488e-10 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 48.4 bits (115), Expect = 8e-10
Identities = 17/59 (28%), Positives = 29/59 (49%)

Query: 62 DEATLFNIAVDPDFQRRGLGRMLLEHLIDELEKRGVVTLWLEVRASNAAAIALYESLGF 120
A + +IAV D++++G+G LL I+ ++ L LE + N +A Y F
Sbjct: 88 GYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4374TCRTETOQM2136e-64 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 213 bits (545), Expect = 6e-64
Identities = 109/452 (24%), Positives = 209/452 (46%), Gaps = 44/452 (9%)

Query: 12 KRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSSQHAKSDWMEMEKQRGISIT 71
K +++H DAGKTT+TE +L AI G+V ++D +E+QRGI+I
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGT----TRTDNTLLERQRGITIQ 57

Query: 72 TSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTR 131
T + F + + VN++DTPGH DF + YR+L+ +D +++I A GV+ +TR L R
Sbjct: 58 TGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 132 LRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFKGVYHLYKDETY 191
P + F+NK+D++ D + +++ +L K +
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVI------------------KQKVE 159

Query: 192 LYQTGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVQGASNEFDEELFLAGEI 251
LY E + + D + + ++ + + LEL Q S F +
Sbjct: 160 LYPNMCVTNFTESEQWDTVIEGN-DDLLEKYMSGKSLEALELEQEES-----IRFHNCSL 213

Query: 252 TPVFFGTALGNFGVDHMLDGLVAWAPAPMPRQTDTRTVEASEEKFTGFVFKIQANMDPKH 311
PV+ G+A N G+D++++ + + + + G VFKI+ K
Sbjct: 214 FPVYHGSAKNNIGIDNLIEVITNKFYSS---------THRGQSELCGKVFKIE--YSEK- 261

Query: 312 RDRVAFMRVVSGKYEKGMKLRQVRTGKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHG 371
R R+A++R+ SG +R K + I++ T + G+ +++AY G+I+ L N
Sbjct: 262 RQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKIDKAYSGEIVILQNEF 320

Query: 372 TIQIGDTFTQGEMMKFTGIPNFA-PELFRRIRLKDPLKQKQLLKGLVQLSEEG-AVQVFR 429
+++ +++ P L + P +++ LL L+++S+ ++ +
Sbjct: 321 -LKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYV 379

Query: 430 PISNNDLIVGAVGVLQFDVVVARLKSEYNVEA 461
+ +++I+ +G +Q +V A L+ +Y+VE
Sbjct: 380 DSATHEIILSFLGKVQMEVTCALLQEKYHVEI 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4376CHANLCOLICIN270.004 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 27.0 bits (59), Expect = 0.004
Identities = 16/49 (32%), Positives = 20/49 (40%), Gaps = 8/49 (16%)

Query: 10 WGIIFLVIALIA--------AALGFGGLAGTAAGAAKIVFVVGIVLFLV 50
W +FL + A AL F LAGT G I V GI+ +
Sbjct: 460 WKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYI 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SPA4378UREASE290.022 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 28.9 bits (65), Expect = 0.022
Identities = 32/141 (22%), Positives = 51/141 (36%), Gaps = 37/141 (26%)

Query: 6 IDTHCHFDFPPFTGDERASIQRACEAGVEKIIVPATEAA-------------HFPRVLAL 52
+D+H HF P I+ A +G+ ++ T A H R++
Sbjct: 133 MDSHIHFICP-------QQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIARMIEA 185

Query: 53 AARFPSLYAALGLHPIVIERHVDDDPDKLQQALAQQQNVVAVGEIGLDLYRDDPQFARQE 112
A FP A G + P AL + V G L L+ D +
Sbjct: 186 ADAFPMNLAFAG-------KGNASLPG----ALVEM---VLGGATSLKLHED---WGTTP 228

Query: 113 RLLDAQLQLAKRYDLPVILHS 133
+D L +A YD+ V++H+
Sbjct: 229 AAIDCCLSVADEYDVQVMIHT 249



 
Contact Sachin Pundhir for Bugs/Comments.
For best view 1024 x 768 resolution & IE 6.0 or above recommended.