PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome

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A) Input parameters
Genome	sequence.gb	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in HE579073 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

SAI8T7_1000090

SAI8T7_1000540

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1000090	2	13	2.318871	Amino acid permease
SAI8T7_1000100	4	14	2.259430	Putative homoserine-o-acetyltransferase
SAI8T7_1000110	6	16	2.069609	Putative uncharacterized protein
SAI8T7_1000120	7	16	2.265987	Putative uncharacterized protein
SAI8T7_1000130	8	17	2.127422	Replicative DNA helicase
SAI8T7_1000140	8	18	1.632741	Adenylosuccinate synthetase
SAI8T7_1000170	4	17	2.322553	**Response regulator
SAI8T7_1000180	3	17	2.250801	Sensor protein kinase walK
SAI8T7_1000190	1	17	2.806363	Putative uncharacterized protein
SAI8T7_1000200	-2	12	1.265369	Putative uncharacterized protein
SAI8T7_1000210	-3	12	1.380986	Putative uncharacterized protein
SAI8T7_1000220	-1	12	0.015644	5'-nucleotidase
SAI8T7_1000230	1	15	-2.644080	Putative uncharacterized protein
SAI8T7_1000240	2	13	-3.561537	Ribosomal RNA large subunit methyltransferase H
SAI8T7_1000250	2	12	-3.503346	Putative uncharacterized protein SA0024
SAI8T7_1000260	0	12	-3.104242	Glycerophosphoryl diester phosphodiesterase
SAI8T7_1000270	0	12	-3.021076	Penicillin binding protein 2 prime
SAI8T7_1000280	0	16	-2.048999	Methicillin-resistance MecR1 regulatory protein
SAI8T7_1000290	-1	17	-0.921644	MW0034 protein
SAI8T7_1000300	-1	14	-1.611023	Putative uncharacterized protein
SAI8T7_1000310	-2	13	-1.577651	Putative uncharacterized protein
SAI8T7_1000320	-3	13	-1.571238	Cassette chromosome recombinase B1
SAI8T7_1000330	-2	14	-2.060547	Cassette chromosome recombinase A1
SAI8T7_1000340	1	14	-3.369116	Putative uncharacterized protein
SAI8T7_1000350	3	16	-4.872253	Putative uncharacterized protein
SAI8T7_1000360	3	16	-4.505663	Metallo-beta-lactamase family protein
SAI8T7_1000370	4	16	-5.252601	Putative uncharacterized protein
SAI8T7_1000380	8	19	-6.366616	Putative uncharacterized protein
SAI8T7_1000390	8	17	-6.148122	Putative uncharacterized protein
SAI8T7_1000400	7	18	-5.504262	Glycosyl transferase, group 1 family protein
SAI8T7_1000410	6	16	-4.236084	Putative uncharacterized protein
SAI8T7_1000420	6	17	-3.305871	Putative uncharacterized protein
SAI8T7_1000430	4	13	-2.048607	Putative uncharacterized protein
SAI8T7_1000440	3	10	-0.337575	Putative uncharacterized protein SA0080
SAI8T7_1000450	1	10	-0.966141	Putative uncharacterized protein SA0082
SAI8T7_1000460	0	11	-0.870206	Putative uncharacterized protein
SAI8T7_1000470	0	12	-0.867438	Similar to sulfide-quinone reductase
SAI8T7_1000480	0	13	-1.141924	Probable tRNA-dihydrouridine synthase
SAI8T7_1000490	1	15	-2.398227	Putative uncharacterized protein
SAI8T7_1000500	3	15	-2.507726	Putative uncharacterized protein
SAI8T7_1000510	6	18	-2.930266	Putative uncharacterized protein
SAI8T7_1000520	8	19	-2.984907	1-phosphatidylinositol phosphodiesterase
SAI8T7_1000530	5	18	-2.381547	Putative uncharacterized protein
SAI8T7_1000540	2	13	-1.165551	Putative lipoprotein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000170	HTHFIS	94	2e-24	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 94.1 bits (234), Expect = 2e-24 Identities = 33/129 (25%), Positives = 66/129 (51%), Gaps = 2/129 (1%) Query: 1 MQMARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYEEEPDIVLLDIMLPG 60 M A ++V DD+ I +L L + GYDV + I + D+V+ D+++P Sbjct: 1 MTGA-TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPD 59 Query: 61 RDGMEVCREVRKKYE-MPIIMLTAKDSEIDKVLGLELGADDYVTKPFSTRELIARVKANL 119 + ++ ++K +P+++++A+++ + + E GA DY+ KPF ELI + L Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119 Query: 120 RRHYSQPAQ 128 +P++ Sbjct: 120 AEPKRRPSK 128
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000370	PF01206	63	4e-15	SirA family protein
>PF01206#SirA family protein Length = 76 Score = 63.2 bits (154), Expect = 4e-15 Identities = 19/70 (27%), Positives = 37/70 (52%) Query: 119 KTFNYSNLQCPGPIVNISKEIKNIAIGDQIEVVVTDHGFLNDIKSWVKQTGHTLVRLNDF 178 ++ + + L CP PI+ K + + G+ + V+ TD G + D +S+ KQTGH L+ + Sbjct: 6 QSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKEE 65 Query: 179 GNEIRAIIQK 188 +++ Sbjct: 66 DGTYHFRLKR 75
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000380	VACCYTOTOXIN	28	0.034	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 28.5 bits (63), Expect = 0.034 Identities = 18/48 (37%), Positives = 27/48 (56%), Gaps = 7/48 (14%) Query: 119 LALILMFIKVTPSTSHIKFNRVLLI--TIGGI-----IGLVSGIVGAG 159 LAL+ + +TP SH F ++I +GGI +G VSG++G G Sbjct: 17 LALVGALVSITPQQSHAAFFTTVIIPAIVGGIATGAAVGTVSGLLGWG 64
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000450	PF01206	61	4e-14	SirA family protein
>PF01206#SirA family protein Length = 76 Score = 60.5 bits (147), Expect = 4e-14 Identities = 20/70 (28%), Positives = 37/70 (52%) Query: 118 KQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDIKSWVKQTRHTLVKLDEN 177 + + G CP PI+K + + + G+ + V TDPG D +S+ KQT H L++ E Sbjct: 6 QSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKEE 65 Query: 178 NNGINAIIQK 187 + + +++ Sbjct: 66 DGTYHFRLKR 75
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000500	GPOSANCHOR	39	7e-05	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 39.3 bits (91), Expect = 7e-05 Identities = 18/134 (13%), Positives = 50/134 (37%), Gaps = 5/134 (3%) Query: 515 INSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKESNKGFINK 574 ++ ++++ L E +++ D ++ +I+ L ++++ +G +N Sbjct: 76 LSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNF 135 Query: 575 LKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEKLIKQLTKVQL 634 + K EK L ++ +LEK + + + + L + ++ Sbjct: 136 ST-----ADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEA 190 Query: 635 QLDELNSQLQELEA 648 + EL L+ Sbjct: 191 RQAELEKALEGAMN 204

SAI8T7_1000630

SAI8T7_1000880

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1000630	2	12	-1.046292	Integral membrane protein DUF6
SAI8T7_1000640	1	12	-0.186214	HTH-type transcriptional regulator NorG
SAI8T7_1000650	1	16	1.482226	Putative uncharacterized protein
SAI8T7_1000660	2	16	1.454260	L-lactate permease homologue
SAI8T7_1000670	1	17	1.883168	Gram-positive signal peptide protein, YSIRK
SAI8T7_1000680	-2	10	1.428782	Transcriptional regulator, MarR family
SAI8T7_1000690	-1	9	2.536286	Lipoprotein
SAI8T7_1000700	0	10	2.961472	Lipoprotein
SAI8T7_1000710	0	11	2.694207	Iron-regulated ABC transporter
SAI8T7_1000720	1	15	3.136375	Probable siderophore biosynthesis protein SbnA
SAI8T7_1000730	3	16	3.489799	Probable ornithine cyclodeaminase protein
SAI8T7_1000740	2	16	3.356143	Putative uncharacterized protein
SAI8T7_1000750	2	17	3.591111	Putative uncharacterized protein
SAI8T7_1000760	0	15	2.722747	Putative uncharacterized protein
SAI8T7_1000770	0	14	3.295728	Similar to siderophore biosynthesis protein
SAI8T7_1000780	-1	14	2.656087	Putative uncharacterized protein
SAI8T7_1000790	-2	13	1.873213	Probable diaminopimelate decarboxylase protein
SAI8T7_1000800	-2	11	1.277577	Putative uncharacterized protein sbnI
SAI8T7_1000810	-1	11	0.019912	Putative uncharacterized protein
SAI8T7_1000820	1	11	-0.255790	Diacetyl reductase [(S)-acetoin forming]
SAI8T7_1000830	2	12	-1.048501	Polysaccharide biosynthesis family protein
SAI8T7_1000840	3	11	-0.619802	Similar to capsular polysaccharide biosynthesis
SAI8T7_1000850	3	11	-0.474623	Putative glycosyltransferase
SAI8T7_1000860	3	11	-0.114336	Putative uncharacterized protein
SAI8T7_1000870	4	11	0.094585	Putative uncharacterized protein
SAI8T7_1000880	3	15	1.599117	Superoxide dismutase [Mn/Fe] 2

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000710	FERRIBNDNGPP	69	2e-15	Ferrichrome-binding periplasmic protein signature.
>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature. Length = 296 Score = 68.8 bits (168), Expect = 2e-15 Identities = 46/191 (24%), Positives = 78/191 (40%), Gaps = 38/191 (19%) Query: 53 PKRVVTLYQGATDVAVSLGVKPVGAVES-----WTQKPKFEYIKNDLKDTKI-VGQEPAP 106 P R+V L ++ ++LG+ P G ++ W +P L D+ I VG P Sbjct: 35 PNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPP-------LPDSVIDVGLRTEP 87 Query: 107 NLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKD----------TTKLMG 156 NLE ++++KP +V S + L++IAP F F D + M Sbjct: 88 NLELLTEMKPSFMVWS-AGYGPSPEMLARIAPGR----GFNFSDGKQPLAMARKSLTEMA 142 Query: 157 KALGKEKEAEDLLKKYDDKVAAFQKDAKAKY--KDAWPLKASVVNF-RADHTRIYA-GGY 212 L + AE L +Y+D F + K ++ + A PL + H ++ Sbjct: 143 DLLNLQSAAETHLAQYED----FIRSMKPRFVKRGARPL--LLTTLIDPRHMLVFGPNSL 196 Query: 213 AGKILNDLGFK 223 +IL++ G Sbjct: 197 FQEILDEYGIP 207
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000730	SYCECHAPRONE	31	0.002	Gram-negative bacterial type III secretion SycE cha...
>SYCECHAPRONE#Gram-negative bacterial type III secretion SycE chaperone signature. Length = 130 Score = 31.2 bits (70), Expect = 0.002 Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 1/33 (3%) Query: 21 VDALTEALTAHAHNDFVQ-PLKPYLRQDPENGH 52 +D E T +HN F Q LKP L D GH Sbjct: 54 LDNNDEKETLLSHNIFSQDILKPILSWDEVGGH 86
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000740	PF04183	316	e-103	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 316 bits (812), Expect = e-103 Identities = 119/527 (22%), Positives = 208/527 (39%), Gaps = 46/527 (8%) Query: 79 RVSKQPLTAAEFWQTIANMNCDLSHEWEVARVEEGLTTAATQLAKQLSELDLASHPFV-- 136 R + +P+ A + + +S +++ T L + L++ + Sbjct: 66 RCADEPVLAQTLLMQLKQVL-SMSDATVAEHMQDLYATLLGDLQLLKARRGLSASDLINL 124 Query: 137 -MSEQFASLKDRPFHPLAKEKRGLREADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANI 195 L P K +RG + + Y E +F L AVK+ HMI + Sbjct: 125 NADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEM 184 Query: 196 DELENLTVPIKEQA----TDMLNDQGLSIDDYVLFPVHPWQYQHILPNVFATEISEKLVV 251 D + LT + Q + + + GL +++ PVHPWQ+Q + F + +E +V Sbjct: 185 DIHQLLTAAMDPQEFARFSQVWQENGLD-HNWLPLPVHPWQWQQKIATDFIADFAEGRMV 243 Query: 252 LLPLKFGD-YLSSSSMRSLIDIGAPYN-HVKVPFAMQSLGALRLTPTRYMKNGEQAEQLL 309 L +FGD +L+ S+R+L + +K+P + + R P RY+ G A + L Sbjct: 244 SLG-EFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWL 302 Query: 310 RQLIEKDEALAKYVMV-CDETA-------WWSYMGQDNDIFKDQLGHLTVQLRKYPEVLA 361 +Q+ D L + V E A ++ + + +++ LG V R+ P Sbjct: 303 QQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLG---VIWRENPCRWL 359 Query: 362 KNDTQQLVSMAALAANDRTLYQMICGKDNISKNDVMTLFEDIAQVFLKVTLSFM-QYGAL 420 K D + V MA L D + + S D T + +V + + +YG Sbjct: 360 KPD-ESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVA 418 Query: 421 PELHGQNILLSFEDGRVQKCVLRD-HDTVRIYKPWLTAHQLSLPKYV--VREDTPNTLIN 477 HGQNI L+ ++G Q+ +L+D +R+ K SLP+ V V + Sbjct: 419 LIAHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMD-SLPQEVRDVTSRLSADYLI 477 Query: 478 EDLETFFAYFQTLAVSVNLYAIIDAIQDLFGVSEHELMSLLKQILKNEVATISWVTTDQL 537 DL+T V + I + GV E LL +L + + Q+ Sbjct: 478 HDLQTGHF--------VTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMK-----KHPQM 524 Query: 538 AVRHILFDKQTWPFKQILLP---LLY-QRDSGGGSMPSGLTTVPNPM 580 + R LF +++L L + D G +P+ L + NP+ Sbjct: 525 SERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEDLQNPL 571
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000750	TCRTETA	73	3e-16	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 72.9 bits (179), Expect = 3e-16 Identities = 66/333 (19%), Positives = 131/333 (39%), Gaps = 22/333 (6%) Query: 17 GIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTPLQFVLVRLL 76 GI +A A+ +P+ G L D+ R+ ++L +L G AV +MA + R++ Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105 Query: 77 QGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVTASILGFSALLM 136 G+ G + A+ + ++R + G + + G + GP++GG+ A Sbjct: 106 AGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGF-SPHAPFF 164 Query: 137 SIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQTCRFIIVGVLANF 196 + A + + + G L E+ + SF+ + V A Sbjct: 165 AAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFR--------WARGMTVVAALM 216 Query: 197 AMYGMLTALSPLASSVNHTAIDDR-----SVIGFLQSAF-WTASILSAPLWGRFNDKSYV 250 A++ ++ + + +++ +DR + IG +AF S+ A + G + Sbjct: 217 AVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE 276 Query: 251 KSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSAL--IQSVMFVVVNACHQ-QL 307 + + IA G IL AT +L + +Q+++ V+ Q QL Sbjct: 277 RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQL 336 Query: 308 KGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPA 340 +G+ T+ + I+G L AI + + Sbjct: 337 QGSLAALTS----LTSIVGPLLFTAIYAASITT 365
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000760	PF04183	300	2e-97	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 300 bits (769), Expect = 2e-97 Identities = 109/474 (22%), Positives = 192/474 (40%), Gaps = 52/474 (10%) Query: 11 WLIDGKSKKITTYKEAIARILQHMAQSADNQTA-VQQHMAQIMSDI--DNSIHRTARYLQ 67 ID ++ + +L + Q A V +HM + + + D + + R L Sbjct: 58 LWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLS 117 Query: 68 SNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSEADLEKYAPECHTSFQLHYLAVHQD 127 ++ + + Q L GHP K G+ + LE+YAPE +F+LH+LAV ++ Sbjct: 118 ASDL---INLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKRE 174 Query: 128 -------------VLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFILLPTHPYQINVLR 174 LLT ++ +E ++Q +D +++ LP HP+Q Sbjct: 175 HMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLD-----HNWLPLPVHPWQWQQK- 228 Query: 175 QHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVF--SKALNIYLKLPIHVKITNFIRTND 232 ++ +G + LG GD S+RT+ S+ + +KLP+ + T+ R Sbjct: 229 IATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIP 288 Query: 233 LEQIERTIDAAQVIASVKDE-----------VETPHFKLMFEEGYRALLPNPLGQTVEPE 281 I A++ + V + P + EGY AL P E Sbjct: 289 GRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQ---E 345 Query: 282 MDLLTNSAMIVREGIPNY-HADKDIHVLASLFETMPD-SPISKLAQVIEQSGLAPEAWLE 339 M +I RE + D+ ++A+L E + P+ I++SGL E WL Sbjct: 346 M-----LGVIWRENPCRWLKPDESPVLMATLMECDENNQPL--AGAYIDRSGLDAETWLT 398 Query: 340 CYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEG-ICLSRTIATEKQ 398 ++P+ L G++L AH QN + +K+G+P ++D +G + L + E Sbjct: 399 QLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMD 458 Query: 399 LVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLVA 452 +P V + + A D H L+ V L + + + E +QL+A Sbjct: 459 SLPQEVRDVTSRLSA-DYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQLLA 511
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000770	PF04183	497	e-173	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 497 bits (1282), Expect = e-173 Identities = 142/579 (24%), Positives = 251/579 (43%), Gaps = 40/579 (6%) Query: 1 MHQLVSSLIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVG 60 + +++S L YE + + A Q G + I +++RF AE+ + + I + R Sbjct: 14 VAKMLSELEYEQV--FHAESQ-GDDRYCINLPGAQWRFIAERG-IWGWLWIDAQTLRC-- 67 Query: 61 DEADTTTDYTQLLREAVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFN 120 AD LL + +D + + + +L T L D Q ++ R + N Sbjct: 68 --ADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASDLINLN 125 Query: 121 -DYEFYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVV 179 D + GH K R G+ ++ P++ +L WLA+ ++ + + Sbjct: 126 ADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEMD 185 Query: 180 VNEMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW 239 ++++L + + + F Q + +G N + +PVHPWQ++ I D + G ++ Sbjct: 186 IHQLLTAAMDPQEFARFSQVWQENGLDHNWL-PLPVHPWQWQQKIATDFIADFAEGRMVS 244 Query: 240 LGESDELYHPQQSIRTMSPIDTT-KYYLKVPISITNTSTKRVLAPHTIENAAQITDWLKQ 298 LGE + + QQS+RT++ +K+P++I NTS R + I + WL+Q Sbjct: 245 LGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWLQQ 304 Query: 299 IQQQDMYLKDE----LKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLID 354 + D L L G V + Y +PY+ ++ LGVIWREN L Sbjct: 305 VFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEM---LGVIWRENPCRWLKP 361 Query: 355 EEDAIPFNALYASDKDGLPFIEKWIKQYG--SEAWTKQFLAVAIRPMIHMLYYHGIAFES 412 +E + L D++ P +I + G +E W Q V + P+ H+L +G+A + Sbjct: 362 DESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALIA 421 Query: 413 HAQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIET 472 H QN+ L + G P R+ LKDF +R +E E S +P+ + V S Sbjct: 422 HGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMDS------LPQEVRDVTS------ 469 Query: 473 DDERLVRDFLH---DAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN 529 RL D+L F+ + I + + G+ E+R +Q + ++ Y + P+++ Sbjct: 470 ---RLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMKKHPQMSE 526 Query: 530 -YQHFDLFEPTIQVEKLTTRRL-LSDSELRIHHVTNPLG 566 + F LF P I L +L D + + N L Sbjct: 527 RFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLE 565
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000820	DHBDHDRGNASE	128	4e-38	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 128 bits (323), Expect = 4e-38 Identities = 66/250 (26%), Positives = 113/250 (45%), Gaps = 2/250 (0%) Query: 5 KVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAAALKLSSDGTKAIAIKADVSN 64 K+A +TG AQGIG +A L G +A VD+N E + L ++ A A ADV + Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 65 RDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITEEQFKTVYGVNVAGVLWGIQAAH 124 + + + G ++VN AG+ I ++++E+++ + VN GV ++ Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 125 EQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFAVRGLTQVAAQDLASEGITVNAFAP 184 + G I+ S ++ Y S+K A T+ +LA I N +P Sbjct: 129 KYMMD-RRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 185 GIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQIALGRVSQPEDVSNVVSFLAGKDSDYI 244 G +T M S+ + E F + I L ++++P D+++ V FL + +I Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSL-ETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246 Query: 245 TGQTIIVDGG 254 T + VDGG Sbjct: 247 TMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000830	NUCEPIMERASE	217	9e-71	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 217 bits (554), Expect = 9e-71 Identities = 79/327 (24%), Positives = 139/327 (42%), Gaps = 33/327 (10%) Query: 6 RVLITGGAGFIGSHLVDDL-QQDYDVYVLDNYRTG-----KRENIKSLADDHVF--ELDI 57 + L+TG AGFIG H+ L + + V +DN K+ ++ LA ++D+ Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61 Query: 58 REYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVATLRLLEIIKKYNSHIK 117 + + + + + F+ V ++V S+E P + N+ L +LE + I+ Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK--IQ 119 Query: 118 RFIFASSAAVYGDLPDLPKSDQSLI-LPLSPYAIDKYYGERTTLNYCSLYNIPTAVVKFF 176 ++ASS++VYG +P S + P+S YA K E Y LY +P ++FF Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFF 179 Query: 177 NVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDFVYVYDVVQSVRLIMEH-- 234 V+GP P M K + G RDF Y+ D+ +++ + + Sbjct: 180 TVYGPWGRPDMALFKFTKAML----EGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIP 235 Query: 235 ---------------KDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARKGDI 279 A YNIG + L++ + + + G + + GD+ Sbjct: 236 HADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGDV 295 Query: 280 KHSYADISNL-KALGFVPKYTVETGLK 305 + AD L + +GF P+ TV+ G+K Sbjct: 296 LETSADTKALYEVIGFTPETTVKDGVK 322

SAI8T7_1001200

SAI8T7_1001490

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1001200	2	11	1.309514	Putative aldehyde dehydrogenase AldA
SAI8T7_1001210	1	14	1.159836	Cation efflux family protein
SAI8T7_1001220	-1	11	1.945785	Putative uncharacterized protein
SAI8T7_1001230	-1	12	1.451998	Putative uncharacterized protein
SAI8T7_1001240	0	14	1.504206	Putative uncharacterized protein
SAI8T7_1001250	1	14	1.279741	Putative uncharacterized protein
SAI8T7_1001260	2	14	1.283404	Putative uncharacterized protein
SAI8T7_1001270	2	13	1.165875	Formate dehydrogenase
SAI8T7_1001280	3	14	1.468170	Similar to integral membrane protein LmrP
SAI8T7_1001290	3	13	1.731674	Gramicidin S synthetase 2 related protein
SAI8T7_1001300	2	14	2.429596	Putative uncharacterized protein
SAI8T7_1001310	2	15	2.006495	Putative uncharacterized protein
SAI8T7_1001320	1	15	1.927579	Acetylglutamate kinase
SAI8T7_1001330	0	14	2.270038	Arginine biosynthesis bifunctional protein ArgJ
SAI8T7_1001340	-1	16	2.396710	N-acetyl-gamma-glutamyl-phosphate reductase
SAI8T7_1001350	-1	14	2.432958	Ornithine aminotransferase 1
SAI8T7_1001360	0	15	2.446599	Similar to branched-chain amino acid transport
SAI8T7_1001370	1	12	3.324564	Isochorismatase hydrolase
SAI8T7_1001380	1	11	3.151056	Putative indole-3-pyruvate decarboxylase
SAI8T7_1001390	0	11	2.061049	PTS system glucose-specific EIICBA component
SAI8T7_1001400	0	14	1.055144	Putative uncharacterized protein
SAI8T7_1001410	-1	14	0.369666	N-acetylmuramic acid 6-phosphate etherase
SAI8T7_1001420	-2	15	-0.232952	PTS system EIIBC component MW0166
SAI8T7_1001430	1	16	-1.431049	RpiR family transcriptional regulator
SAI8T7_1001440	1	15	-3.570784	Type-1 restriction enzyme R protein
SAI8T7_1001450	2	18	-5.479838	Putative uncharacterized protein
SAI8T7_1001460	3	19	-5.792263	Putative uncharacterized protein
SAI8T7_1001470	0	15	-4.293212	Similar to ABC transporter ATP-binding protein
SAI8T7_1001480	-1	14	-3.907492	Similar to SA0193/BacI-like protein
SAI8T7_1001490	-1	11	-3.396289	Putative uncharacterized protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001280	TCRTETA	32	0.004	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 32.1 bits (73), Expect = 0.004 Identities = 61/337 (18%), Positives = 127/337 (37%), Gaps = 33/337 (9%) Query: 7 TLKVRLISNFLQLIITTAFIPFIALYLTDMLS----QSIVGIYLVGLVVLKFPLSIISGY 62 L V L + L + +P + L D++ + GI L +++F + + G Sbjct: 6 PLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGA 65 Query: 63 LIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQI-IGFCVAYAIFTIVWGLQFPVMDT 121 L + F ++ ++L+ A + M + LW + IG VA + G V Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMAT--APFLWVLYIGRIVAG-----ITGATGAVAGA 118 Query: 122 LIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGYSMLLLFLIAACIFLIVLFILY 181 I D D + + G +LGGLM G+S F AA + + Sbjct: 119 YIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGC 178 Query: 182 IWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLVLKDRNYMLLISGFSIIMMGEFSI 241 LP+ ++ + + + L+ F + ++G+ Sbjct: 179 FLLPESHKGERRPLRREALNPLASFRWARGMTVVAA--------LMAVFFIMQLVGQVPA 230 Query: 242 SSYIAIRLKDQF--ETISIGSYDITGAKMLAILLMINTVVVILLTYSISKVVLKIDFKKA 299 + + I +D+F + +IG LA +++++ ++T ++ ++ ++A Sbjct: 231 ALW-VIFGEDRFHWDATTIGI-------SLAAFGILHSLAQAMITGPVAA---RLGERRA 279 Query: 300 LITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGEII 336 L+ G++ GY L + + + M++ G I Sbjct: 280 LMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIG 316
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001290	NUCEPIMERASE	53	8e-09	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 52.9 bits (127), Expect = 8e-09 Identities = 54/266 (20%), Positives = 101/266 (37%), Gaps = 55/266 (20%) Query: 2052 NTLLTGATGFLGAYLIEALQGYSHRIYCFIRADNEEIAWYKLMTNLNDYFS----EETVE 2107 L+TGA GF+G ++ + L H++ + D NLNDY+ + +E Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQV---VGID-----------NLNDYYDVSLKQARLE 47 Query: 2108 MM----LSNIEVIVGDFECMDDVVLPENMDTIIH----AGARTDHFGDDDEFEKVNVQGT 2159 ++ ++ + D E M D+ + + + R + + N+ G Sbjct: 48 LLAQPGFQFHKIDLADREGMTDLFASGHFERVFISPHRLAVR-YSLENPHAYADSNLTGF 106 Query: 2160 VDVIRLAQQHH-ARLIYVSTISV-GTYFDIDTEDVTFSEADVYKGQLLTSPYTRSKFYSE 2217 ++++ + + L+Y S+ SV G + FS D + S Y +K +E Sbjct: 107 LNILEGCRHNKIQHLLYASSSSVYG-----LNRKMPFSTDDSVDHPV--SLYAATKKANE 159 Query: 2218 LKVLEAVNN-GLDGRIVRVGNLTSPYNGRWHM------RNIKTNRFSMVMNDLLQLDCIG 2270 L + GL +R + P+ GR M + + + V N Sbjct: 160 LMAHTYSHLYGLPATGLRFFTVYGPW-GRPDMALFKFTKAMLEGKSIDVYNY-------- 210 Query: 2271 VSMAEMPVDFSFVDTTARQIVALAQV 2296 +M DF+++D A I+ L V Sbjct: 211 ---GKMKRDFTYIDDIAEAIIRLQDV 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001300	ENTSNTHTASED	29	0.009	Enterobactin synthetase component D signature.
>ENTSNTHTASED#Enterobactin synthetase component D signature. Length = 234 Score = 29.2 bits (65), Expect = 0.009 Identities = 15/57 (26%), Positives = 27/57 (47%), Gaps = 5/57 (8%) Query: 84 GQP-----IYVSLSYSYPYIVCVVDKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQI 135 QP ++ S+S+ + V+ ++ +GIDIEKI + L ++ QI Sbjct: 76 RQPLWPDGLFGSISHCATTALAVISRQRIGIDIEKIMSQHTATELAPSIIDSDERQI 132
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001320	CARBMTKINASE	32	0.002	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 31.7 bits (72), Expect = 0.002 Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 7/84 (8%) Query: 155 INADTLAYFIASSLKAPIYV-LSNIAGVLIN-----DVVIPQLPLVDIHQYIEHGD-IYG 207 I+ D +A + A I++ L+++ G + + + ++ + ++ +Y E G G Sbjct: 213 IDKDLAGEKLAEEVNADIFMILTDVNGAALYYGTEKEQWLREVKVEELRKYYEEGHFKAG 272 Query: 208 GMIPKVLDAKNAIENGCPKVIIAS 231 M PKVL A IE G + IIA Sbjct: 273 SMGPKVLAAIRFIEWGGERAIIAH 296
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001370	ISCHRISMTASE	60	4e-13	Isochorismatase signature.
>ISCHRISMTASE#Isochorismatase signature. Length = 312 Score = 59.6 bits (144), Expect = 4e-13 Identities = 31/99 (31%), Positives = 51/99 (51%) Query: 86 LDKRDDDFVIDKRHFSAFVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQ 145 L DDD V+ K +SAF T+L +R+ G D +++ G+ HIG TA +A+ + Sbjct: 112 LAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKA 171 Query: 146 FFVTDMMSAQNETLHQFPIDNVFPLMGQTITTNDFLNIL 184 FFV D ++ + HQ ++ T+ T+ L+ L Sbjct: 172 FFVGDAVADFSLEKHQMALEYAAGRCAFTVMTDSLLDQL 210
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001430	DNABINDINGHU	30	0.002	Prokaryotic integration host factor signature.
>DNABINDINGHU#Prokaryotic integration host factor signature. Length = 91 Score = 30.4 bits (69), Expect = 0.002 Identities = 16/75 (21%), Positives = 28/75 (37%), Gaps = 15/75 (20%) Query: 87 ELIENESVETLKNKMIARATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTI 146 +LI +A AT + + +DA A+ L + L GFG + Sbjct: 6 DLIA----------KVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVR- 54 Query: 147 GDLFQKLSRIGLNVR 161 ++ +R G N + Sbjct: 55 ----ERAARKGRNPQ 65

SAI8T7_1002070

SAI8T7_1002120

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1002070	1	18	3.066791	Putative methyltransferase
SAI8T7_1002080	1	16	3.283583	Probable ribokinase
SAI8T7_1002090	2	15	3.621451	D-ribose pyranase
SAI8T7_1002100	3	14	3.279484	Putative ribose uptake protein rbsU
SAI8T7_1002110	3	14	2.287867	Similar to sugar-binding transcriptional
SAI8T7_1002120	2	15	1.341901	Major facilitator transporter

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1002120	TCRTETB	100	4e-25	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 100 bits (251), Expect = 4e-25 Identities = 91/392 (23%), Positives = 158/392 (40%), Gaps = 18/392 (4%) Query: 38 PLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLI 97 P + + PA N + I G ++D++G R+ G+II+ F S++ Sbjct: 38 PDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGF 97 Query: 98 ISDITA-LLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGV 156 + LLI+ R +QG AA + V+ E+ +A + G G+ Sbjct: 98 VGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPA 157 Query: 157 IGGLIATNFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSF 216 IGG+IA W +I ++ L+K ++V + FD KG+ + +V I F Sbjct: 158 IGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEV---RIKGHFDIKGIILMSVGIVFF 214 Query: 217 TLLLTQGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNN 276 L T ++S L + +++ LIF+ R+ PF+D + +N PF+ L Sbjct: 215 MLFTT---------SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCG 265 Query: 277 FVLNSGLGVTVVFFIYA-QTHLGLSAAQSGLVTLPYAIVAVAMIR-LGEKATLRFGGKLM 334 ++ + V Y + LS A+ G V + ++V + +G R G + Sbjct: 266 GIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYV 325 Query: 335 LIIGPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKV 394 L IG F + S + S + + I V G T TI S+ ++ Sbjct: 326 LNIGVTFLSVSFLTASFLLETTSWF---MTIIIVFVLGGLSFTKTVISTIVSSSLKQQEA 382 Query: 395 GLATGLYKMSGTLGGAFGIALSTTVFSMLQLN 426 G L + L GIA+ + S+ L+ Sbjct: 383 GAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLD 414

SAI8T7_1002430

SAI8T7_1002480

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1002430	0	12	3.093866	Putative uncharacterized protein
SAI8T7_1002440	0	11	3.597552	Putative N-acetylmannosamine-6-phosphate
SAI8T7_1002450	0	10	3.416329	Nucleoside recognition domain protein
SAI8T7_1002460	1	11	3.643833	Lipase=2
SAI8T7_1002470	2	12	2.919400	Putative uncharacterized protein
SAI8T7_1002480	1	13	3.046606	Putative trimethylamine dehydrogenase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1002440	PHPHTRNFRASE	27	0.046	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 27.4 bits (61), Expect = 0.046 Identities = 17/82 (20%), Positives = 27/82 (32%), Gaps = 12/82 (14%) Query: 65 DYDHSDVFITATSKEVDELIESQCEVIALDATLQQ---RPKETLDELVSYIRTHAPNVEI 121 D V + T +EV E + + P T D +VE+ Sbjct: 222 DGIEGIVIVNPTEEEVKAYEEKRAAFEKQKQEWAKLVGEPSTTKD---------GAHVEL 272 Query: 122 MADIATVEEAKNAARLGFDYIG 143 A+I T ++ G + IG Sbjct: 273 AANIGTPKDVDGVLANGGEGIG 294
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1002460	GPOSANCHOR	47	1e-07	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 47.4 bits (112), Expect = 1e-07 Identities = 41/309 (13%), Positives = 85/309 (27%), Gaps = 12/309 (3%) Query: 1 MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTSNAAAQKETLNQPGEQG 60 M + R YS+RK G SV A + + +E + + + Sbjct: 1 MTKNNTNRHYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERAD 60 Query: 61 NAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQNSKTIRTQ---NDNQVK 117 + K D E + L ++K + +N K++ + Sbjct: 61 KFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEA 120 Query: 118 QDSERQGSKQSHQN------NATNNTERQNDQVQNTHHAERNGSQSTTSQSNDVDKSQPS 171 + ++ + + + N E + + + + S + Sbjct: 121 RKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKT 180 Query: 172 IPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTHQPAHQIIDAKQD 231 + A+K +A + + + S K + +K + A Sbjct: 181 LEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNF 240 Query: 232 DTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAPKTRSTTNAAADAKK 291 T ++ K + + Q EK KT AA +A+K Sbjct: 241 STADSAKIKTLEAEKAALEARQAELEK---ALEGAMNFSTADSAKIKTLEAEKAALEAEK 297 Query: 292 VRPLKANQV 300 +QV Sbjct: 298 ADLEHQSQV 306

SAI8T7_1003020

SAI8T7_1003310

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1003020	2	16	-1.075687	Exotoxin=6
SAI8T7_1003030	1	15	-1.175554	Exotoxin=7
SAI8T7_1003040	3	14	-1.689685	Putative uncharacterized protein (fragment)
SAI8T7_1003050	4	15	-2.798544	Exotoxin=8
SAI8T7_1003060	2	16	-2.991972	Superantigen-like protein
SAI8T7_1003070	2	15	-1.204044	Superantigen-like protein 5
SAI8T7_1003080	1	13	-1.942571	Exotoxin=11
SAI8T7_1003090	1	13	-2.000277	Superantigen-like protein 7
SAI8T7_1003100	3	9	-1.605994	Exotoxin=13
SAI8T7_1003110	4	9	-1.710183	Exotoxin=14
SAI8T7_1003120	4	11	-1.932228	Type I restriction enzyme EcoR124II M protein
SAI8T7_1003130	10	14	-3.878715	Type I restriction modification DNA specificity
SAI8T7_1003140	10	13	-3.605067	Exotoxin=15
SAI8T7_1003150	11	13	-3.550002	Putative uncharacterized protein
SAI8T7_1003160	13	21	-4.088587	Tandem lipoprotein within Pathogenicity island
SAI8T7_1003170	12	22	-3.884319	Uncharacterized lipoprotein SAV0437
SAI8T7_1003180	12	22	-3.442082	Tandem lipoprotein within Pathogenicity island
SAI8T7_1003190	12	22	-3.554770	Uncharacterized lipoprotein SAV0439
SAI8T7_1003200	11	21	-3.539695	Uncharacterized lipoprotein SA0400
SAI8T7_1003210	12	21	-3.501573	Uncharacterized lipoprotein SAV0441
SAI8T7_1003220	11	19	-3.347690	Tandem lipoprotein within Pathogenicity island
SAI8T7_1003230	7	14	-2.027657	Tandem lipoprotein
SAI8T7_1003240	1	14	-0.576526	Uncharacterized lipoprotein SAOUHSC_00402
SAI8T7_1003250	-1	13	1.640724	Uncharacterized lipoprotein SAV0444
SAI8T7_1003260	-1	13	1.708234	Lipoprotein
SAI8T7_1003270	0	13	2.152735	Putative uncharacterized protein
SAI8T7_1003280	3	16	2.776193	Putative cobalamin synthesis protein
SAI8T7_1003290	2	15	2.474119	NADH dehydrogenase subunit 5
SAI8T7_1003300	1	15	2.589768	UPF0753 protein SaurJH1_0488
SAI8T7_1003310	2	16	1.150839	Putative uncharacterized protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003020	TOXICSSTOXIN	95	3e-26	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 95.5 bits (237), Expect = 3e-26 Identities = 46/214 (21%), Positives = 84/214 (39%), Gaps = 11/214 (5%) Query: 18 TGVITSNVQSVQAKTEVKQQSESELKHYYNKPVLERKNVTGYKYTEKGKDYIDVIVDNQY 77 T V S+ Q ++ + +L +Y+ N + + + + + Sbjct: 25 TPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNS---EVLDNSLGSMRIKNTDGS 81 Query: 78 SQISLVGSDKDKFKDGDNSNIDVFILREGDSRQATN-----YSIGGVTKTNSQPFIDYIH 132 + + S +D+ R S+ + + I GVT T P I Sbjct: 82 ISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLP--TPIE 139 Query: 133 TPILEIKKGKEEPQSSLYQIYKEDISLKELDYRLRERAIKQHGLYSNGLKQGQI-TITMK 191 P+ GK+ P + K+ +++ LD+ +R + + HGLY + K G ITM Sbjct: 140 LPLKVKVHGKDSPLKYGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMN 199 Query: 192 DGKSHTIDLSQKLEKERMGDSIDGRQIQKILVEM 225 DG ++ DLS+K E I+ +I+ I E+ Sbjct: 200 DGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003030	TOXICSSTOXIN	80	3e-21	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 80.5 bits (198), Expect = 3e-21 Identities = 32/168 (19%), Positives = 63/168 (37%), Gaps = 18/168 (10%) Query: 1 MNIIDGNSVNNLALIGKDKQHYHTGVHRNLNIFYVN-----EDKRFEGAKYSIGGITSAN 55 M I + + +L + +++ + I G+T+ Sbjct: 73 MRIKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTE 132 Query: 56 DKA--VDLIAEARVIKADHIGEYDYDFFPFKIDKEAMSLKEIDFKLRKYLIDNYGLYGEM 113 ++L + +V D +Y F DK+ +++ +DF++R L +GLY Sbjct: 133 KLPTPIELPLKVKVHGKDSPLKYGPKF-----DKKQLAISTLDFEIRHQLTQIHGLYRSS 187 Query: 114 ST----GKITVKKKYYGKYTFELDKKLQEDRMSDVINVTDIDRIEIKV 157 KIT+ Y +L KK + + IN+ +I IE ++ Sbjct: 188 DKTGGYWKITMNDG--STYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003050	TOXICSSTOXIN	89	5e-24	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 88.9 bits (220), Expect = 5e-24 Identities = 28/128 (21%), Positives = 48/128 (37%), Gaps = 5/128 (3%) Query: 67 VFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSVTKKDNQGMISRDVSEYMITKEEIS 126 ++ + + G+T T + L V + K++++ Sbjct: 109 TKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYG---PKFDKKQLA 165 Query: 127 LKELDFKLRKQLIEKHNLYGNM--GSGTIVIKMKNGGKYTFELHKKLQEHRMADVIEGTN 184 + LDF++R QL + H LY + G I M +G Y +L KK + + I Sbjct: 166 ISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDE 225 Query: 185 IDKIEVNI 192 I IE I Sbjct: 226 IKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003060	TOXICSSTOXIN	101	8e-28	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 101 bits (253), Expect = 8e-28 Identities = 45/216 (20%), Positives = 81/216 (37%), Gaps = 13/216 (6%) Query: 87 TKVETPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNIVPDYF 146 T V + K N KDL +Y+ S F N ++ ++R N Sbjct: 25 TPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTN-SEVLDNSLGSMRIKNTDGSI- 82 Query: 147 IYKIALVGKDDKKYDEGVHRNVDVFVVLEEKNKYGVE----RYSVGGITKSNSKKVDHKA 202 + + VD+ +K+++ E + + G+T + + Sbjct: 83 --SLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIEL 140 Query: 203 GVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIENHNLYGNV--GSGKIVIN 260 +++ + + K K+Q+++ LDF++R QL + H LY + G I Sbjct: 141 PLKVKVHGKDSPLKYG---PKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKIT 197 Query: 261 MKNGGKYTFELHKKLQENRMADVIDGTNIDNIEVNI 296 M +G Y +L KK + N I+ I IE I Sbjct: 198 MNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003070	TOXICSSTOXIN	135	2e-41	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 135 bits (340), Expect = 2e-41 Identities = 49/201 (24%), Positives = 73/201 (36%), Gaps = 14/201 (6%) Query: 44 NVTKDIFDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKAR 103 + +I DL D+YS S N S G + + IF Sbjct: 41 STNDNIKDLLDWYSSGSDTFTNSEVLDNSLGS---MRIKNTDGSISLIIFPSPYYSPAFT 97 Query: 104 KNPGLDI-----FVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIAT 158 K +D+ + F GVT + I P +K D Sbjct: 98 KGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLP--TPIELPLK-VKVHGKDSPLK 154 Query: 159 YGRVHYIYKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKI-KVIMKDGGYYTFELNKKL 217 YG K+++++ LDF++R L Q LY+ K K+ M DG Y +L+KK Sbjct: 155 YG--PKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKF 212 Query: 218 QTNRMSDVIDGRNIEKIEANI 238 + N I+ I+ IEA I Sbjct: 213 EYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003080	TOXICSSTOXIN	192	1e-63	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 192 bits (488), Expect = 1e-63 Identities = 49/197 (24%), Positives = 82/197 (41%), Gaps = 16/197 (8%) Query: 42 DIKDLYRYYSSESFEFSNI--------SGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDK 93 +IKDL +YSS S F+N S +++N +GS + F G+ Sbjct: 45 NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGE---- 100 Query: 94 YKKGLEGQNVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSF 153 K L + + + + GVT + L V KV+G + Sbjct: 101 -KVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKV-KVHGKDSPLKYGP- 157 Query: 154 LINKEEVSLKELDFKIRKQLVEKYGLYKGTTKYGKI-TINLKDEKKEVIDLGDKLQFERM 212 +K+++++ LDF+IR QL + +GLY+ + K G I + D DL K ++ Sbjct: 158 KFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTE 217 Query: 213 GDVLNSKDIQNIAVTIN 229 +N +I+ I IN Sbjct: 218 KPPINIDEIKTIEAEIN 234
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003090	TOXICSSTOXIN	125	2e-37	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 125 bits (314), Expect = 2e-37 Identities = 47/199 (23%), Positives = 73/199 (36%), Gaps = 19/199 (9%) Query: 44 DTNKLHQYYSGPSYELTNV--------SGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENK 95 + L +YS S TN S + + S L+ F G+ Sbjct: 45 NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGE---- 100 Query: 96 YKEKTHGLDVFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSID 155 K + + F +SGVT L L K+ KD + Sbjct: 101 -KVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELP----LKVKVHGKDSP-LK 154 Query: 156 EFFFIQKEEVSLKELDFKIRKLLIKKYKLYEGSA-DKGRIVINMKDENKYEIDLSDKLDF 214 K+++++ LDF+IR L + + LY S G I M D + Y+ DLS K ++ Sbjct: 155 YGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEY 214 Query: 215 ERMADVINSEQIKNIEVNL 233 IN ++IK IE + Sbjct: 215 NTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003100	TOXICSSTOXIN	130	1e-39	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 130 bits (329), Expect = 1e-39 Identities = 39/197 (19%), Positives = 69/197 (35%), Gaps = 15/197 (7%) Query: 43 INMLHQYYSEESFESTNISVKSEDYYGSNVLNFNQRNKTFKVFLLGDDKNKY------KE 96 I L +YS S TN V + + + + + K Sbjct: 46 IKDLLDWYSSGSDTFTNSEVLD---NSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKV 102 Query: 97 KTHGLDVFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKHGFSINELF 156 + + + + G+T + P L+VK F Sbjct: 103 DLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLP--TPIELP-LKVKVHGKDSPLKYGPKF 159 Query: 157 FIQKEEVSLKELDFKIRKMLVEKYRLYKGAS-DKGRIVINMKDEKKYVIDLSEKLSFDRM 215 K+++++ LDF+IR L + + LY+ + G I M D Y DLS+K ++ Sbjct: 160 --DKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTE 217 Query: 216 FDVMDSKQIKNIEVNLN 232 ++ +IK IE +N Sbjct: 218 KPPINIDEIKTIEAEIN 234
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003110	TOXICSSTOXIN	193	4e-64	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 193 bits (491), Expect = 4e-64 Identities = 51/202 (25%), Positives = 92/202 (45%), Gaps = 10/202 (4%) Query: 31 KQNQKSVNKHDKEALYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKF 90 K + S N + K+ L Y +G + N L + ++R K I +++ + Sbjct: 36 KTAKASTNDNIKDLLDWYSSG-SDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSP 94 Query: 91 QQRSYEGLDVFFVQEKRDKHD-----IFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAF 145 E +D+ + K+ +H I + + GV K + P L + K G+D+ Sbjct: 95 AFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELP-LKV-KVHGKDSP 152 Query: 146 VKGYPYYIKKEKITLKELDYKLRKHLIEKYGLYKTISKDGRV-KISLKDGSFYNLDLRSK 204 +K Y K+++ + LD+++R L + +GLY++ K G KI++ DGS Y DL K Sbjct: 153 LK-YGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKK 211 Query: 205 LKFKYMGEVIESKQIKDIEVNL 226 ++ I +IK IE + Sbjct: 212 FEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003140	TOXICSSTOXIN	108	4e-31	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 108 bits (270), Expect = 4e-31 Identities = 47/225 (20%), Positives = 86/225 (38%), Gaps = 19/225 (8%) Query: 16 LTTGMITTTAQPVKASTLEVRSQAT-------QDLSEYYKGRGFELTNVTGYKYG-NKVT 67 L T PV S+ ++ A +DL ++Y TN + Sbjct: 15 LLLATTATDFTPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNSEVLDNSLGSMR 74 Query: 68 FIDNSQQIDVTLTGNE----KLTVKDDDEVSNVDVFVVREGSDKSAITTSIGGITKTNGT 123 + I + + + T + +++ + S+ + I I G+T T Sbjct: 75 IKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTE-- 132 Query: 124 QHKDTVQNVNLSVSKSTGQHTTSVTSEYYSIYKEEISLKELDFKLRKHLIDKHDLYKTEP 183 T + L V K G+ S K+++++ LDF++R L H LY++ Sbjct: 133 -KLPTPIELPLKV-KVHGK--DSPLKYGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSD 188 Query: 184 KDSKI-RITMKNGGYYTFELNKKLQPHRMGDTIDSRNIEKIEVNL 227 K +ITM +G Y +L+KK + + I+ I+ IE + Sbjct: 189 KTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003200	BCTERIALGSPC	31	0.002	Bacterial general secretion pathway protein C signa...
>BCTERIALGSPC#Bacterial general secretion pathway protein C signature. Length = 272 Score = 31.5 bits (71), Expect = 0.002 Identities = 17/83 (20%), Positives = 34/83 (40%), Gaps = 9/83 (10%) Query: 184 INSNVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPILKMHIDGDLKGSSVGYKKLEIDF 243 +N VP Y+AK D V Q + RY + + + S G +++ Sbjct: 124 VNEEVPGYNAKIVSIRPDRVVLQYQGRYEV---------LGLYSQEDSGSDGVPGAQVNE 174 Query: 244 SKEENSELSIVDSLNFQPAKNKD 266 ++ + ++ D ++F P N + Sbjct: 175 QLQQRASTTMSDYVSFSPIMNDN 197
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003240	BCTERIALGSPC	35	3e-04	Bacterial general secretion pathway protein C signa...
>BCTERIALGSPC#Bacterial general secretion pathway protein C signature. Length = 272 Score = 34.6 bits (79), Expect = 3e-04 Identities = 18/83 (21%), Positives = 34/83 (40%), Gaps = 9/83 (10%) Query: 177 INENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVLKMHIDGDLKGSSVGYKKLEIDF 236 +NE VP Y+AK D V Q + RY + + + S G +++ Sbjct: 124 VNEEVPGYNAKIVSIRPDRVVLQYQGRYEV---------LGLYSQEDSGSDGVPGAQVNE 174 Query: 237 SKGEKSDLSVIDSLNFQPAKVDE 259 +++ ++ D ++F P D Sbjct: 175 QLQQRASTTMSDYVSFSPIMNDN 197
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003260	BCTERIALGSPC	29	0.025	Bacterial general secretion pathway protein C signa...
>BCTERIALGSPC#Bacterial general secretion pathway protein C signature. Length = 272 Score = 28.8 bits (64), Expect = 0.025 Identities = 17/83 (20%), Positives = 33/83 (39%), Gaps = 9/83 (10%) Query: 201 INENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKSPVLKMHIDGNLKGSSVGDRKLEIDF 260 +NE VP Y+AK D V Q + RY + + + S G +++ Sbjct: 124 VNEEVPGYNAKIVSIRPDRVVLQYQGRYEV---------LGLYSQEDSGSDGVPGAQVNE 174 Query: 261 SKRENSHLSVIDSLDYQPAKVDE 283 ++ + ++ D + + P D Sbjct: 175 QLQQRASTTMSDYVSFSPIMNDN 197

SAI8T7_1004070

SAI8T7_1004310

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1004070	2	14	0.482410	Putative TrmH family tRNA/rRNA
SAI8T7_1004080	2	15	0.167784	Putative uncharacterized protein
SAI8T7_1004090	3	22	2.286925	Putative uncharacterized protein
SAI8T7_1004100	4	28	2.929455	Transcription antitermination protein NusG
SAI8T7_1004110	4	30	3.083986	50S ribosomal protein L1
SAI8T7_1004120	4	32	3.543366	50S ribosomal protein L10
SAI8T7_1004130	5	35	3.900909	Putative uncharacterized protein SA0499
SAI8T7_1004140	4	34	3.839032	DNA-directed RNA polymerase subunit beta
SAI8T7_1004150	2	33	3.410681	DNA-directed RNA polymerase subunit beta'
SAI8T7_1004160	1	31	3.472121	30S ribosomal protein S7
SAI8T7_1004170	0	23	2.551012	Elongation factor G
SAI8T7_1004180	0	15	1.590508	Elongation factor Tu
SAI8T7_1004190	2	13	0.611565	Aminoacylase
SAI8T7_1004200	2	12	0.515831	Putative pyridoxal phosphate-dependent
SAI8T7_1004210	2	13	0.402366	Molecular chaperone Hsp31 and glyoxalase 3
SAI8T7_1004220	2	13	-0.519064	Ribulokinase
SAI8T7_1004230	0	11	-0.333110	Uncharacterized epimerase/dehydratase SACOL0599
SAI8T7_1004240	-1	12	-1.099289	Probable branched-chain-amino-acid
SAI8T7_1004250	2	13	-0.239297	Putative phosphoglycolate phosphatase
SAI8T7_1004260	4	18	-0.092767	Deoxypurine kinase
SAI8T7_1004270	4	17	-0.328749	Deoxynucleoside kinase
SAI8T7_1004280	5	18	0.687528	Putative uncharacterized protein
SAI8T7_1004290	6	19	0.737880	FMN-dependent NADPH-azoreductase
SAI8T7_1004300	5	17	0.397149	Serine-aspartate repeat-containing protein C
SAI8T7_1004310	3	15	-0.480159	Serine-aspartate repeat-containing protein D

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1004170	TCRTETOQM	619	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 619 bits (1598), Expect = 0.0 Identities = 170/671 (25%), Positives = 299/671 (44%), Gaps = 66/671 (9%) Query: 31 KTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRGITITSAAT 90 K NIG++AH+DAGKTT TE +LY +G I ++G +G ++ D E+ RGITI + T Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 91 TAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGV 150 + WE +VNIIDTPGH+DF EV RSL VLDGA+ ++ A+ GV+ QT ++ G+ Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121 Query: 151 PRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVEMKCFKYTN 210 P I F+NK+D+ G + + ++L A Q K Y N Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ------------------KVELYPN 163 Query: 211 DLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSELKEAIRQAT 270 T E E + V E +D+L+EKY+ + + EL++ Sbjct: 164 MCVTNFTESE---------------QWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRF 208 Query: 271 TNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVIAKADDSAE 330 N +PV G+A N G+ +++ + + S +E Sbjct: 209 HNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH--------------------RGQSE 248 Query: 331 FAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDT 390 FK+ +L + R+YSG + V+ S K K ++ + +ID Sbjct: 249 LCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKIDK 307 Query: 391 VYSGDIAAAVG----LKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQDKMT 446 YSG+I L GDT + + I E P P++ +VEP ++ + Sbjct: 308 AYSGEIVILQNEFLKLNSV-LGDTKLLPQRERI----ENPLPLLQTTVEPSKPQQREMLL 362 Query: 447 QALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMVSYRE 506 AL+++ + DP + D T ++I+ +G++ +++ ++++++VE + P V Y E Sbjct: 363 DALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYME 422 Query: 507 TFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAG 566 + + + + + + +P G+G ++E+++ G + + + +V G Sbjct: 423 R--PLKKAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEG 480 Query: 567 LKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPM 626 ++ E G L G+ + D K G Y+ S+ F++ A + L++ KK +LEP Sbjct: 481 IRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPY 539 Query: 627 MKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQG 686 + I P+EY+ D + + + N +++ +P + Y + L T G Sbjct: 540 LSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNG 599 Query: 687 RGTYTMYFDHY 697 R Y Sbjct: 600 RSVCLTELKGY 610
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1004180	TCRTETOQM	86	2e-20	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 86.1 bits (213), Expect = 2e-20 Identities = 52/149 (34%), Positives = 81/149 (54%), Gaps = 7/149 (4%) Query: 14 NIGTIGHVDHGKTTLTAAI---ATVLAKNGDSVAQSYDMIDNAPEEKERGITINTSHIEY 70 NIG + HVD GKTTLT ++ + + + G SV + DN E++RGITI T + Sbjct: 5 NIGVLAHVDAGKTTLTESLLYNSGAITELG-SVDKGTTRTDNTLLERQRGITIQTGITSF 63 Query: 71 QTDKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRNVGVPA 130 Q + +D PGH D++ + + +DG IL++SA DG QTR R +G+P Sbjct: 64 QWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT 123 Query: 131 LVVFLNKVDMVDDEELLELVEMEVRDLLS 159 + F+NK+D + L V ++++ LS Sbjct: 124 -IFFINKIDQNGID--LSTVYQDIKEKLS 149
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1004230	NUCEPIMERASE	93	1e-23	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 92.9 bits (231), Expect = 1e-23 Identities = 77/328 (23%), Positives = 126/328 (38%), Gaps = 42/328 (12%) Query: 3 KIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPVQNGPFEIL---------- 52 K ++TGA G IG + + E V+ D D ++ E+L Sbjct: 2 KYLVTGAAGFIGFHVSKRLLE--AGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59 Query: 53 DVTDRDRMFELVRDFEADSLMHMAALLSAT-AEKNPILAWDLNMGGLMNALEAARTYNL- 110 D+ DR+ M +L + + L+ + +NP D N+ G +N LE R + Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119 Query: 111 HFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRFGVDTRSVRFP 170 H SS +G + + ++ P ++Y K A EL+ Y +G+ +RF Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRF- 178 Query: 171 GLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYM-DMMYMDDAIEAIIKLMEA- 228 V P G D A+ + KA+ EG + G D Y+DD EAII+L + Sbjct: 179 ---FTVYGP-WGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVI 234 Query: 229 --DDAKLETRNG-----------YNLSAMSFDPEMVKEAIQ--EYYPNFTLDYDVDPIRQ 273 D + G YN+ S P + + IQ E ++ P++ Sbjct: 235 PHADTQWTVETGTPAASIAPYRVYNIGNSS--PVELMDYIQALEDALGIEAKKNMLPLQP 292 Query: 274 GIANSWPDS-IDTSCSRGEWGFDPKYDL 300 G ++ DT GF P+ + Sbjct: 293 GDV---LETSADTKALYEVIGFTPETTV 317
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1004310	GPOSANCHOR	36	0.001	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 36.2 bits (83), Expect = 0.001 Identities = 27/200 (13%), Positives = 59/200 (29%), Gaps = 8/200 (4%) Query: 22 KFSIRKYTVGTASILVGTTLI-FGLGNQ-EAKAAESTNKELNE--ATTSASDNQSSDKVD 77 +S+RK GTAS+ V T++ GL +A +T + + +D + Sbjct: 9 HYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADKFEIENNT 68 Query: 78 MQQLNQEDNTKNDNQKEMVSSQGNET-TSNGNKSIEKESVQSTTGNKVEVSTAKSDEQAS 136 ++ N + + N K+ E + +S+ E+ K+D + + Sbjct: 69 LKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKA 128 Query: 137 PKSTNEDLNTKQTISNQEGLQPDLLENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIK 196 + + L + ++ T + +A K Sbjct: 129 LEGAMNFSTADSAKIKTLEAEKAALAAR---KADLEKALEGAMNFSTADSAKIKTLEAEK 185 Query: 197 SNAETLVDNNSNSNNENNAD 216 + E + Sbjct: 186 AALEARQAELEKALEGAMNF 205

SAI8T7_1004710

SAI8T7_1004850

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1004710	1	13	-3.022826	Periplasmic binding protein
SAI8T7_1004720	1	13	-3.458323	Transport system permease protein
SAI8T7_1004730	0	13	-3.612555	L-2-haloalkanoic acid dehalogenase
SAI8T7_1004740	0	13	-3.687743	MW0576 protein
SAI8T7_1004750	2	12	-3.047839	Putative uncharacterized protein
SAI8T7_1004760	2	11	-2.450569	Putative uncharacterized protein
SAI8T7_1004770	2	12	-2.259607	Putative esterase/lipase
SAI8T7_1004780	1	11	-1.914146	Putative uncharacterized protein
SAI8T7_1004790	-1	10	-1.772849	Putative uncharacterized protein
SAI8T7_1004800	0	9	-1.732532	Putative antiporter subunit mnhA2
SAI8T7_1004810	0	10	-1.848298	Putative antiporter subunit mnhD2
SAI8T7_1004820	1	10	-2.177194	Putative antiporter subunit mnhE2
SAI8T7_1004830	1	11	-1.534494	Monovalent cation/H+ antiporter subunit G
SAI8T7_1004840	1	10	-1.811177	Putative uncharacterized protein
SAI8T7_1004850	2	11	-1.960363	Transposase

SAI8T7_1005010

SAI8T7_1005240

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1005010	4	9	1.165170	Ferrichrome transport permease
SAI8T7_1005020	3	9	0.247415	Iron-hydroxamate transport permease
SAI8T7_1005030	0	9	-0.076097	Putative dihydroxyacetone kinase
SAI8T7_1005040	-1	9	-0.894833	Putative uncharacterized protein
SAI8T7_1005050	-1	10	-1.563061	Putative uncharacterized protein
SAI8T7_1005060	-2	9	-1.849522	Putative uncharacterized protein MW0616
SAI8T7_1005070	-2	11	-2.168194	SA0610 protein
SAI8T7_1005080	-2	12	-2.998513	Putative uncharacterized protein
SAI8T7_1005090	-3	12	-3.622045	Putative uncharacterized protein
SAI8T7_1005100	-3	8	-2.672065	Response regulator protein graR
SAI8T7_1005110	0	10	-1.706438	Sensor histidine kinase graS
SAI8T7_1005120	0	10	-1.782226	ABC transporter, ATP-binding protein
SAI8T7_1005130	-1	8	-3.249860	ABC transporter permease
SAI8T7_1005140	0	9	-2.028610	Putative pit accessory protein
SAI8T7_1005150	0	9	-1.503835	SA0619 protein
SAI8T7_1005160	-1	11	-2.730141	Secretory antigen SsaA homologue
SAI8T7_1005170	-1	15	-3.548779	Putative Transporter
SAI8T7_1005180	-1	13	-2.938515	Similar to AraC/XylS family transcriptional
SAI8T7_1005190	2	14	-1.140573	Probable transcriptional regulatory protein
SAI8T7_1005200	2	12	-2.433506	Putative uncharacterized protein
SAI8T7_1005210	3	12	-2.334828	Putative uncharacterized protein
SAI8T7_1005220	3	10	-1.442845	MW0634 protein
SAI8T7_1005230	4	11	-1.278947	Transporter, major facilitator family protein
SAI8T7_1005240	2	16	-1.835246	Putative uncharacterized protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1005080	SACTRNSFRASE	47	3e-09	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 47.2 bits (112), Expect = 3e-09 Identities = 20/97 (20%), Positives = 35/97 (36%), Gaps = 3/97 (3%) Query: 50 EYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIGQALLDAI 109 Y+ K F+ + +G + + + + D+ V K Y+ +G+G ALL Sbjct: 58 SYVEEEGKAAFLYYLENNCIGRIKIRSN-WNGYALIE--DIAVAKDYRKKGVGTALLHKA 114 Query: 110 MAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGV 146 + W N + N A + F I V Sbjct: 115 IEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAV 151
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1005100	HTHFIS	64	5e-14	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 63.7 bits (155), Expect = 5e-14 Identities = 26/111 (23%), Positives = 57/111 (51%), Gaps = 1/111 (0%) Query: 3 ILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYW 62 IL+ +DD + L + L + ++V + + + + ++V+ DV +P + F Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65 Query: 63 CRKMREV-SNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQ 112 ++++ ++P+L +S+++ M + + E GA DY+ KPF LI + Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1005120	PF05272	36	1e-04	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 36.2 bits (83), Expect = 1e-04 Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 8/56 (14%) Query: 57 GPSGSGKTTLLNVLSSIDYISQGSITLKGKK--LEKLSNK------ELSDIRKHDI 104 G G GK+TL+N L +D+ S + K E+++ E++ R+ D Sbjct: 603 GTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSEMTAFRRADA 658
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1005230	TCRTETA	58	3e-11	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 57.5 bits (139), Expect = 3e-11 Identities = 73/365 (20%), Positives = 134/365 (36%), Gaps = 41/365 (11%) Query: 35 KNYKLFVA--NMFLLGMGIAVTVPYLVLFATKDLGMTTNQ---YGLLLASAAISQFTVNS 89 N L V + L +GI + +P L +DL + + YG+LLA A+ QF Sbjct: 3 PNRPLIVILSTVALDAVGIGLIMPVLP-GLLRDLVHSNDVTAHYGILLALYALMQFACAP 61 Query: 90 IIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTIWLFILLYAIFQGLFAPAMPQLYA 149 ++ SD F R+ +++++L A+ ++I +W+ + + I G+ Sbjct: 62 VLGALSD--RFGRRPVLLVSLAGAAVDYAIMATAPFLWV-LYIGRIVAGITGATGA---V 115 Query: 150 SARESINVSSSKDRAQFANTVLRSMFSLGFLFGPFIGAQLIGLKGYAGLFGGTISIILFT 209 + +++ +RA+ + + F G + GP +G + G +A F L Sbjct: 116 AGAYIADITDGDERARHFG-FMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNF 174 Query: 210 LVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKDKTL--------LLPFIAFILLHIGQWM 261 L + ++ + + A N L + FI+ +GQ Sbjct: 175 LTGCFLLPESHK-----GERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVP 229 Query: 262 YTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIIL-GVLSSRLHTRTLLIYGAIFGG 320 + +F D + +G + L ++ G +++RL R L+ G I G Sbjct: 230 AAL-WVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADG 288 Query: 321 LFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQDILPDFPGYASTLFSNAMVIGQ 380 Y + +M F + L GIG +P S GQ Sbjct: 289 TGYILLAFATRGWM-----AFPIMVLLASGGIG-------MPALQAMLSRQVDEERQ-GQ 335 Query: 381 LGGNL 385 L G+L Sbjct: 336 LQGSL 340 Score = 49.4 bits (118), Expect = 1e-08 Identities = 44/186 (23%), Positives = 73/186 (39%), Gaps = 13/186 (6%) Query: 239 MFKDKTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQ--HVGYLASLCAGLEVPFM 296 M ++ L++ L +G + +P + D + N+ H G L +L A ++ Sbjct: 1 MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACA 60 Query: 297 IILGVLSSRLHTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISY 356 +LG LS R R +L+ + Y + +++ G++ I A G +Y Sbjct: 61 PVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAG-AY 119 Query: 357 FQDILPD-----FPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGM 411 DI G+ S F MV G + G L+GG H FF +AA L Sbjct: 120 IADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH-----APFFAAAALNGLNF 174 Query: 412 ILIFFT 417 + F Sbjct: 175 LTGCFL 180

SAI8T7_1006040

SAI8T7_1006330

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1006040	2	19	2.245305	Nucleoside-diphosphate sugar epimerase
SAI8T7_1006050	2	21	2.745992	Putative uncharacterized protein
SAI8T7_1006060	3	30	2.935445	Glycolytic operon regulator
SAI8T7_1006070	3	34	2.444332	Glyceraldehyde-3-phosphate dehydrogenase 1
SAI8T7_1006080	1	28	1.780237	Phosphoglycerate kinase
SAI8T7_1006090	1	19	1.502026	Triosephosphate isomerase
SAI8T7_1006100	1	16	0.555954	2,3-bisphosphoglycerate-independent
SAI8T7_1006110	0	14	-0.582274	Enolase
SAI8T7_1006120	0	11	-1.341374	Putative membrane spanning protein
SAI8T7_1006130	3	11	-0.486227	Putative Carboxyesterase homologue
SAI8T7_1006140	6	14	1.607096	Ribonuclease R
SAI8T7_1006150	7	15	0.784605	Putative uncharacterized protein
SAI8T7_1006160	5	15	1.109777	Putative uncharacterized protein
SAI8T7_1006170	5	15	1.254877	Putative uncharacterized protein
SAI8T7_1006180	4	14	1.209504	Transposase for insertion sequence element IS256
SAI8T7_1006190	4	15	0.626807	Clumping factor A
SAI8T7_1006200	0	12	-2.151795	Coagulase family protein
SAI8T7_1006210	1	13	-1.650844	Putative Extracellular matrix protein-binding
SAI8T7_1006220	-1	15	-1.933508	Putative uncharacterized protein
SAI8T7_1006230	-1	12	-1.550712	Thermonuclease
SAI8T7_1006240	0	12	-1.827080	Putative uncharacterized protein
SAI8T7_1006250	-1	12	-1.750278	Phosphoglycerate mutase family protein
SAI8T7_1006260	1	16	-2.120414	Similar to transporter, LysE family
SAI8T7_1006270	5	21	-1.091789	Putative uncharacterized protein
SAI8T7_1006280	7	22	-0.282687	3-dehydroquinate dehydratase
SAI8T7_1006290	9	25	-0.266086	Putative uncharacterized protein
SAI8T7_1006300	9	26	-0.112363	Putative uncharacterized protein truncated-SA
SAI8T7_1006310	8	24	0.275741	Transposase A from transposon Tn554
SAI8T7_1006320	6	21	0.842260	Transposase B from transposon Tn554
SAI8T7_1006330	4	16	0.276107	Streptomycin=3''-adenylyltransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006050	IGASERPTASE	30	0.010	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.010 Identities = 24/162 (14%), Positives = 62/162 (38%), Gaps = 5/162 (3%) Query: 49 SNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQQNQYQY 108 S + N +++ + ++ +++A E + +E ++ + + +AN Q+ + Sbjct: 1034 SETTETVAENSKQESKTVEKNEQDA-TETTAQNREVAKEAKSNVKANTQTNEVAQSGSET 1092 Query: 109 VPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKSDDNQKKTDDNKQPAQPKPQP- 167 Q + ++E K +++++ SQ Q +T + + P Sbjct: 1093 KETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPT 1152 Query: 168 ---QQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNA 206 ++P + N Q ++ Q ++STT N+ Sbjct: 1153 VNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNS 1194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006170	ALARACEMASE	27	0.049	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 26.7 bits (59), Expect = 0.049 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 2/37 (5%) Query: 135 MYDIYP-PYDGIPDEAFLI-KELKVNSLAGKTGTINY 169 D+ P P GI L KE+K++ +A GT+ Y Sbjct: 305 AVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGY 341
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006190	ICENUCLEATIN	43	7e-06	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 42.8 bits (100), Expect = 7e-06 Identities = 72/369 (19%), Positives = 131/369 (35%), Gaps = 6/369 (1%) Query: 514 SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD 573 S G+DS + S +G +ST +G S + SD + S + DS+ Sbjct: 294 STGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLI 353 Query: 574 SDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSD 633 + S + DS + S + SD + S + +DS+ + S + + Sbjct: 354 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 413 Query: 634 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 693 S + S + SD + S + DS + S + DS + S Sbjct: 414 STQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQT 473 Query: 694 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 753 + SD + S S + +S + S + S + S + ++SD + Sbjct: 474 AQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYG 533 Query: 754 SDSDSDSDSD------SDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSD 807 S S + ++S S + +S + S + SD + S + SDS Sbjct: 534 STSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSII 593 Query: 808 SESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD 867 + S + S + S + S +G S S++ +DS + GS + + Sbjct: 594 AGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYN 653 Query: 868 SNSDSESGS 876 S + GS Sbjct: 654 SILTAGYGS 662 Score = 42.8 bits (100), Expect = 7e-06 Identities = 74/379 (19%), Positives = 135/379 (35%), Gaps = 6/379 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + E+S G S + S +G ST +G DS+ + S + DS Sbjct: 309 GSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSL 368 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + SD + S + +DS+ + S + +S + S + Sbjct: 369 TAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQK 428 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 SD + S + DS + S + DS + S + SD + S S Sbjct: 429 GSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTS 488 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD----- 739 + +S + S + S + S + ++SD + S S + ++S Sbjct: 489 TAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGY 548 Query: 740 -SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDS 798 S + +S + S + SD + S + SDS + S + S Sbjct: 549 GSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSL 608 Query: 799 DSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDT 858 + S + S + S S + +DS + S +G +S ++ S T+ Sbjct: 609 TAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQE 668 Query: 859 GSDNDSDSDSNSDSESGSN 877 GSD + S S + + S+ Sbjct: 669 GSDLTAGYGSTSTAGADSS 687 Score = 42.0 bits (98), Expect = 1e-05 Identities = 69/363 (19%), Positives = 125/363 (34%), Gaps = 6/363 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + EDS G S + S +G ST +G+DS+ + S + +S Sbjct: 261 GSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQ 320 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + SD + S + DS+ + S + DS + S + Sbjct: 321 TAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQK 380 Query: 625 DSDSDSDSDSDSDSDSDSD------SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 678 SD + S + +DS S + +S + S + SD + S Sbjct: 381 GSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTG 440 Query: 679 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 738 + DS + S + DS + S + SD + S S + +S + Sbjct: 441 TAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGY 500 Query: 739 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDS 798 S + S + S + ++SD + S S + ++S + S + +S Sbjct: 501 GSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVL 560 Query: 799 DSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDT 858 + S + SD + S + SDS + S + S ++ S T+ Sbjct: 561 TAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTARE 620 Query: 859 GSD 861 S Sbjct: 621 QSV 623 Score = 42.0 bits (98), Expect = 1e-05 Identities = 72/369 (19%), Positives = 132/369 (35%), Gaps = 6/369 (1%) Query: 514 SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD 573 S G DS + S +G DS+ +G S + SD + S + +DS+ Sbjct: 342 STGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLI 401 Query: 574 SDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSD 633 + S + +S + S + SD + S + DS+ + S + D Sbjct: 402 AGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGED 461 Query: 634 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 693 S + S + SD + S S + +S + S + S + S Sbjct: 462 SSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQT 521 Query: 694 SDSDSDSDSDSDSDSDSDSDSD------SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 747 + ++SD + S S + ++S S + +S + S + SD + Sbjct: 522 AQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYG 581 Query: 748 SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSD 807 S + SDS + S + S + S + S + S S + +DS Sbjct: 582 STGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLI 641 Query: 808 SESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD 867 + S + +S + S + SD +G S S++ +DS + GS + + Sbjct: 642 AGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYN 701 Query: 868 SNSDSESGS 876 S + GS Sbjct: 702 SILTAGYGS 710 Score = 41.3 bits (96), Expect = 2e-05 Identities = 76/379 (20%), Positives = 138/379 (36%), Gaps = 6/379 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSA------SDSDSASDSDS 558 G + EDS G S + S +G ST +G+DS+ S + +S Sbjct: 357 GSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQ 416 Query: 559 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 618 + S + SD + S + DS+ + S + DS + S + Sbjct: 417 TAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQK 476 Query: 619 ASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 678 SD + S S + +S + S + S + S + ++SD + S S Sbjct: 477 GSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTS 536 Query: 679 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 738 + ++S + S + +S + S + SD + S + SDS + Sbjct: 537 TAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGY 596 Query: 739 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDS 798 S + S + S + S + S S + +DS + S + +S Sbjct: 597 GSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSIL 656 Query: 799 DSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDT 858 + S ++ SD + S S + +DS + S +G +S ++ S T+ Sbjct: 657 TAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQE 716 Query: 859 GSDNDSDSDSNSDSESGSN 877 GSD S S S + + S+ Sbjct: 717 GSDLTSGYGSTSTAGADSS 735 Score = 41.3 bits (96), Expect = 2e-05 Identities = 74/372 (19%), Positives = 133/372 (35%), Gaps = 2/372 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + + SD G S + DS +G ST +G DS+ + S + SD Sbjct: 229 GSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 288 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + + S + S + +S + S + SD + S + Sbjct: 289 TAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGD 348 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + DS + S + SD + S + +DS + S Sbjct: 349 DSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQ 408 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + +S + S + SD + S + DS + S + DS + Sbjct: 409 TAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGY 468 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD + S S + +S + S + S + S + ++SD Sbjct: 469 GSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDL 528 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 + S S + ++S + S + +S +G S ++ SD T+ GS + Sbjct: 529 ITGYGSTSTAGANSSLIAGYGSTQTASYNSV--LTAGYGSTQTAREGSDLTAGYGSTGTA 586 Query: 865 DSDSNSDSESGS 876 SDS+ + GS Sbjct: 587 GSDSSIIAGYGS 598 Score = 40.9 bits (95), Expect = 3e-05 Identities = 74/369 (20%), Positives = 135/369 (36%), Gaps = 6/369 (1%) Query: 514 SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA-- 571 S G+DS + S +G +ST +G S + SD + S + DS+ Sbjct: 390 STGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLI 449 Query: 572 ----SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSD 627 S + DS + S + SD + S S + +S+ + S + Sbjct: 450 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYG 509 Query: 628 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 687 S + S + ++SD + S S + ++S + S + +S + S Sbjct: 510 STLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQT 569 Query: 688 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 747 + SD + S + SDS + S + S + S + S + Sbjct: 570 AREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYG 629 Query: 748 SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSD 807 S S + +DS + S + +S + S ++ SD + S S + +DS Sbjct: 630 STSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLI 689 Query: 808 SESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD 867 + S + +S + S + SD SG S S++ +DS + GS + Sbjct: 690 AGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYH 749 Query: 868 SNSDSESGS 876 S+ + GS Sbjct: 750 SSLTAGYGS 758 Score = 40.9 bits (95), Expect = 3e-05 Identities = 71/378 (18%), Positives = 130/378 (34%), Gaps = 4/378 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G E + S G S + +DS +G ST +G +S+ + S SD Sbjct: 181 GSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQMAGYGSTQTGMKGSDL 240 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + S + S + DS+ + S + SD + S + + Sbjct: 241 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 300 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + +S + S + SD + S + DS + S Sbjct: 301 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 360 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + DS + S + SD + S + +DS + S + +S + Sbjct: 361 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGY 420 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD + S + DS + S + +S + S + SD Sbjct: 421 GSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 480 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSD----SDSTSDTGS 860 + S S + +S + S + S + GS + ++SD STS G+ Sbjct: 481 TAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGA 540 Query: 861 DNDSDSDSNSDSESGSNN 878 ++ + S + N+ Sbjct: 541 NSSLIAGYGSTQTASYNS 558 Score = 39.4 bits (91), Expect = 8e-05 Identities = 70/381 (18%), Positives = 126/381 (33%), Gaps = 14/381 (3%) Query: 510 IPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDS--------------ASDSDSASD 555 +P D D +SGS + + + ST S +S + Sbjct: 138 LPVTDDIDATIESGSTQPTQTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYG 197 Query: 556 SDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASD 615 S + +DS + S + +S + S SD + S + DS+ Sbjct: 198 STGTAGADSTLVAGYGSTQTAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLI 257 Query: 616 SDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 675 + S + DS + S + SD + S + +DS + S + + Sbjct: 258 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 317 Query: 676 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 735 S + S + SD + S + DS + S + DS + S Sbjct: 318 STQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQT 377 Query: 736 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSD 795 + SD + S + +DS + S + +S + S ++ SD + Sbjct: 378 AQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYG 437 Query: 796 SDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDST 855 S + DS + S + DS + S + SD +G S S++ +S Sbjct: 438 STGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLI 497 Query: 856 SDTGSDNDSDSDSNSDSESGS 876 + GS + S + GS Sbjct: 498 AGYGSTQTAGYGSTLTAGYGS 518 Score = 38.6 bits (89), Expect = 1e-04 Identities = 73/381 (19%), Positives = 139/381 (36%), Gaps = 4/381 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + E+S G S + S +G ST +G DS+ + S + DS Sbjct: 405 GSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSL 464 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + SD + S S + +S+ + S + S + S + + Sbjct: 465 TAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQN 524 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 +SD + S S + ++S + S + +S + S + SD + S Sbjct: 525 ESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTG 584 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + SDS + S + S + S + S + S S + +DS + Sbjct: 585 TAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGY 644 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + +S + S + SD + S S + ++S + S + +S Sbjct: 645 GSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSIL 704 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS----DSDSTSDTGS 860 + S + SD S S S + +DS+ + GS +S S ST Sbjct: 705 TAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTARE 764 Query: 861 DNDSDSDSNSDSESGSNNNVV 881 + + S S +G++++++ Sbjct: 765 QSVLTTGYGSTSTAGADSSLI 785 Score = 37.4 bits (86), Expect = 4e-04 Identities = 70/375 (18%), Positives = 131/375 (34%), Gaps = 6/375 (1%) Query: 513 DSDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDS 570 S + GS + S +G ST +G+DS + S + +S + S Sbjct: 171 THQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQMAGYGS 230 Query: 571 ASDSDSASDSDSASDSDSASDSDSA----SDSDSASDSDSASDSDSASDSDSASDSDSDS 626 SD + S + DS+ S + DS+ + S + SD + Sbjct: 231 TQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTA 290 Query: 627 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 686 S + +DS + S + +S + S + SD + S + DS Sbjct: 291 GYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDS 350 Query: 687 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 746 + S + DS + S + SD + S + +DS + S + Sbjct: 351 SLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTA 410 Query: 747 DSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDS 806 +S + S + SD + S + DS + S + DS + S Sbjct: 411 GEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 470 Query: 807 DSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDS 866 + SD + S S + +S + S + S+ + ST +++D + Sbjct: 471 TQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLIT 530 Query: 867 DSNSDSESGSNNNVV 881 S S +G+N++++ Sbjct: 531 GYGSTSTAGANSSLI 545 Score = 37.0 bits (85), Expect = 4e-04 Identities = 75/372 (20%), Positives = 135/372 (36%), Gaps = 2/372 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + + SD G S + DS +G ST +G DS+ + S + SD Sbjct: 421 GSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 480 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S S + S + S + S + S + ++SD + S S + + Sbjct: 481 TAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGA 540 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 +S + S + +S + S + SD + S + SDS + S Sbjct: 541 NSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQ 600 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + S + S + S + S S + +DS + S + +S + Sbjct: 601 TASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGY 660 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD + S S + +DS + S + S + S + SD Sbjct: 661 GSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDL 720 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 S S S + +DS + S + S+ +G S ++ S T+ GS + + Sbjct: 721 TSGYGSTSTAGADSSLIAGYGSTQTASYHSS--LTAGYGSTQTAREQSVLTTGYGSTSTA 778 Query: 865 DSDSNSDSESGS 876 +DS+ + GS Sbjct: 779 GADSSLIAGYGS 790 Score = 37.0 bits (85), Expect = 4e-04 Identities = 75/373 (20%), Positives = 135/373 (36%), Gaps = 2/373 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + + SD G S S + +S +G ST +G S + S + ++SD Sbjct: 469 GSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDL 528 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S S + + S + S + +S + S + SD + S + S Sbjct: 529 ITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGS 588 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + S + S + S + S S + +DS + S Sbjct: 589 DSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQ 648 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + +S + S + SD + S S + +DS + S + +S + Sbjct: 649 TAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGY 708 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD S S S + +DS + S + S + S + S Sbjct: 709 GSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVL 768 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 + S S + +DS + S + S +G S ++ SD T+ GS + + Sbjct: 769 TTGYGSTSTAGADSSLIAGYGSTQTAGYHSI--LTAGYGSTQTAQERSDLTTGYGSTSTA 826 Query: 865 DSDSNSDSESGSN 877 +DS+ + GS Sbjct: 827 GADSSLIAGYGST 839 Score = 37.0 bits (85), Expect = 4e-04 Identities = 67/339 (19%), Positives = 124/339 (36%), Gaps = 2/339 (0%) Query: 514 SDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA 571 DS + GS + GSD +G STS +G +S+ + S + S + S Sbjct: 460 EDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGST 519 Query: 572 SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSD 631 + + SD + S S + ++S+ + S ++ +S + S + SD + Sbjct: 520 QTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAG 579 Query: 632 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 691 S + SDS + S + S + S + S + S S + +DS Sbjct: 580 YGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSS 639 Query: 692 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 751 + S + +S + S + SD + S S + +DS + S + Sbjct: 640 LIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAG 699 Query: 752 SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD 811 +S + S + SD S S S + ++S + S + S + S Sbjct: 700 YNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGST 759 Query: 812 SDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + S + S S + +DS+ + GS + S Sbjct: 760 QTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHS 798 Score = 33.6 bits (76), Expect = 0.005 Identities = 56/335 (16%), Positives = 107/335 (31%) Query: 527 NSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDS 586 S +D + + + S ++ D D+ +S S + Sbjct: 97 TKTSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPT 156 Query: 587 DSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDS 646 + + S S + S + +S + S + +DS + S Sbjct: 157 QTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQ 216 Query: 647 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 706 + +S + S SD + S + DS + S + DS + Sbjct: 217 TAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGY 276 Query: 707 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 766 S + SD + S + +DS + S + +S + S + SD Sbjct: 277 GSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDL 336 Query: 767 DSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSESDSDS 826 + S + DS + S + DS + S ++ SD + S + + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 827 DSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSD 861 DS + S +G +S ++ S T+ GSD Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSD 431 Score = 33.2 bits (75), Expect = 0.005 Identities = 66/339 (19%), Positives = 125/339 (36%), Gaps = 2/339 (0%) Query: 514 SDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA 571 S + GS + + SD +G STS +G++S+ + S ++ +S + S Sbjct: 508 YGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGST 567 Query: 572 SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSD 631 + SD + S + SDS+ + S ++ S + S + S + Sbjct: 568 QTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTG 627 Query: 632 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 691 S S + +DS + S + +S + S + SD + S S + +DS Sbjct: 628 YGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSS 687 Query: 692 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 751 + S + +S + S + SD S S S + +DS + S + Sbjct: 688 LIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTAS 747 Query: 752 SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD 811 S + S + S + S S + ++S + S + S + S Sbjct: 748 YHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGST 807 Query: 812 SDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + SD + S S + +DS+ + GS + +S Sbjct: 808 QTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNS 846 Score = 32.8 bits (74), Expect = 0.008 Identities = 74/372 (19%), Positives = 135/372 (36%), Gaps = 2/372 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + +SD G S S + ++S +G ST + +S + S + SD Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDL 576 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + S S + S + S+ + S + S + S S + + Sbjct: 577 TAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGA 636 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + +S + S + SD + S S + +DS + S Sbjct: 637 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQ 696 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + +S + S + SD S S S + +DS + S + S + Sbjct: 697 TAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGY 756 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + S + S S + +DS + S + S + S + SD Sbjct: 757 GSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDL 816 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 + S S + +DS + S + +S +G S ++ +SD T+ GS + + Sbjct: 817 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSI--LTAGYGSTQTAQENSDLTTGYGSTSTA 874 Query: 865 DSDSNSDSESGS 876 DS+ + GS Sbjct: 875 GYDSSLIAGYGS 886 Score = 32.8 bits (74), Expect = 0.008 Identities = 67/360 (18%), Positives = 131/360 (36%) Query: 522 SGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSD 581 +G S +G S + ++S + S S + ++S+ + S ++ +S + Sbjct: 506 AGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYG 565 Query: 582 SASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSD 641 S + SD + S + SDS+ + S ++ S + S + S Sbjct: 566 STQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLT 625 Query: 642 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 701 + S S + +DS + S + +S + S + SD + S S + +D Sbjct: 626 TGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGAD 685 Query: 702 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 761 S + S + +S + S + SD S S S + +DS + S Sbjct: 686 SSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQT 745 Query: 762 SDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSE 821 + S + S + S + S S + +DS + S + S + Sbjct: 746 ASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYG 805 Query: 822 SDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSDSNSDSESGSNNNVV 881 S + SD + S S + +DSS + ST G ++ + S + N+++ Sbjct: 806 STQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLT 865 Score = 31.6 bits (71), Expect = 0.018 Identities = 77/387 (19%), Positives = 138/387 (35%), Gaps = 14/387 (3%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSA--------------SDS 550 G + E SD G S + SDS +G ST + S+ S Sbjct: 565 GSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVL 624 Query: 551 DSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDS 610 + S S + +DS+ + S + +S + S + SD + S S + + Sbjct: 625 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGA 684 Query: 611 DSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 670 DS+ + S + +S + S + SD S S S + +DS + S Sbjct: 685 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQ 744 Query: 671 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 730 + S + S + S + S S + +DS + S + S + Sbjct: 745 TASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGY 804 Query: 731 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDS 790 S + SD + S S + +DS + S + +S + S ++ +SD Sbjct: 805 GSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDL 864 Query: 791 DSDSDSDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + S S + DS + S + +S + S + SD +G S S++ Sbjct: 865 TTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 924 Query: 851 DSDSTSDTGSDNDSDSDSNSDSESGSN 877 +S + GS + S + GS+ Sbjct: 925 ESSLIAGYGSTQTASFKSTLMAGYGSS 951 Score = 31.3 bits (70), Expect = 0.026 Identities = 68/339 (20%), Positives = 125/339 (36%), Gaps = 2/339 (0%) Query: 514 SDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA 571 +S + GS + GSD +G ST +GSDS+ + S ++ S + S Sbjct: 556 YNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGST 615 Query: 572 SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSD 631 + S + S S + +DS+ + S + +S + S + SD + Sbjct: 616 QTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAG 675 Query: 632 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 691 S S + +DS + S + +S + S + SD S S S + +DS Sbjct: 676 YGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSS 735 Query: 692 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 751 + S + S + S + S + S S + +DS + S + Sbjct: 736 LIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAG 795 Query: 752 SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD 811 S + S + SD + S S + ++S + S + +S + S Sbjct: 796 YHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGST 855 Query: 812 SDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + +SD + S S + DS+ + GS + +S Sbjct: 856 QTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNS 894 Score = 31.3 bits (70), Expect = 0.026 Identities = 67/360 (18%), Positives = 128/360 (35%) Query: 522 SGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSD 581 + +S +G S + S + S + SDS+ + S ++ S + Sbjct: 554 ASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYG 613 Query: 582 SASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSD 641 S + S + S S + +DS+ + S + +S + S + SD Sbjct: 614 STQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLT 673 Query: 642 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 701 + S S + +DS + S + +S + S + SD S S S + +D Sbjct: 674 AGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGAD 733 Query: 702 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 761 S + S + S + S + S + S S + +DS + S Sbjct: 734 SSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQT 793 Query: 762 SDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSE 821 + S + S + SD + S S + +DS + S + +S + Sbjct: 794 AGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYG 853 Query: 822 SDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSDSNSDSESGSNNNVV 881 S + +SD + S S + DSS + ST G ++ + S + N+++ Sbjct: 854 STQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLT 913
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006200	IGASERPTASE	31	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.8 bits (69), Expect = 0.016 Identities = 28/162 (17%), Positives = 52/162 (32%), Gaps = 4/162 (2%) Query: 261 ALKLKADTEAAKNDVSKRSKRSLNTQNNKST-TQEISEEQKAEYQRKSEALKERFINRQK 319 +KA+T+ + S + T K T T E E+ K E ++ E K Sbjct: 1073 KSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVT-SQVSP 1131 Query: 320 SKNESVVSLIDDEDDNENDRQLVVSAPSKKPTTPTTYTETTTQVPMPTVERQTQQQIVYK 379 + +S E END + + P + T + + + T+ V Sbjct: 1132 KQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNT 1191 Query: 380 TPKPLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALP 421 + N E+ TT + + + ++P Sbjct: 1192 GNSVVE--NPENTTPATTQPTVNSESSNKPKNRHRRSVRSVP 1231
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006240	PF05704	28	0.035	Capsular polysaccharide synthesis protein
>PF05704#Capsular polysaccharide synthesis protein Length = 307 Score = 27.5 bits (61), Expect = 0.035 Identities = 13/69 (18%), Positives = 24/69 (34%), Gaps = 7/69 (10%) Query: 116 EWVKKNYENTNHRYLVTLNLNSK-------KFTYCTKIIYQAYKFGVSEKSVKSYGLHII 168 W + Y N + +++ N + + YK + +Y HI Sbjct: 239 YWKEIPYVNNVNPHMLQYLGNLPYDNSMFNYIKSTSPVQKLTYKLDYNNLKRNTYYDHIF 298 Query: 169 SPYAIKDNF 177 S +KDN+ Sbjct: 299 SIDKLKDNY 307
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006270	SACTRNSFRASE	31	0.001	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 31.1 bits (70), Expect = 0.001 Identities = 19/91 (20%), Positives = 34/91 (37%), Gaps = 6/91 (6%) Query: 53 IVFGCYENETLIATAALEQI--RYVGKEHKSLIKYNFVTNNDKSINSELINFIINYARQN 110 F Y I + Y E ++ K K + + L++ I +A++N Sbjct: 66 AAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAK----DYRKKGVGTALLHKAIEWAKEN 121 Query: 111 NYESLLTSIVSNNIGAKVFYSALGFDILGFE 141 ++ L+ NI A FY+ F I + Sbjct: 122 HFCGLMLETQDINISACHFYAKHHFIIGAVD 152

SAI8T7_1006820

SAI8T7_1006970

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1006820	0	9	-3.009045	Similar to hydrolase, haloacid dehalogenase-like
SAI8T7_1006830	1	11	-4.004782	Putative O-acetyltransferase MW0856
SAI8T7_1006840	1	12	-4.364554	Chaperone protein ClpB
SAI8T7_1006850	4	17	-5.698086	Putative uncharacterized protein
SAI8T7_1006860	3	15	-2.826499	HMGL-like protein
SAI8T7_1006870	3	14	-1.779588	Putative uncharacterized protein
SAI8T7_1006880	1	11	-0.646008	Putative uncharacterized protein
SAI8T7_1006890	0	11	1.636458	Putative uncharacterized protein
SAI8T7_1006900	-1	10	1.614920	3-oxoacyl-[acyl-carrier-protein] synthase 3
SAI8T7_1006910	0	11	0.977187	3-oxoacyl-[acyl-carrier-protein] synthase 2
SAI8T7_1006920	1	10	-0.354410	Oligopeptide transport system permease protein
SAI8T7_1006930	1	10	-0.690431	Oligopeptide transport system permease protein
SAI8T7_1006940	2	11	-1.118185	Oligopeptide transport system ATP-binding
SAI8T7_1006950	3	12	-2.123458	Oligopeptide transport system ATP-binding
SAI8T7_1006960	3	14	-2.358895	Similar to peptide binding protein OppA
SAI8T7_1006970	3	13	-2.194237	Similar to oligopeptide ABC

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006840	IGASERPTASE	36	7e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 36.2 bits (83), Expect = 7e-04 Identities = 17/143 (11%), Positives = 48/143 (33%), Gaps = 14/143 (9%) Query: 420 QLEIEESALKNESDNASKQRLQELQEELANEKEKQAALQSRVESEKEKIANLQEKRAQLD 479 E+ +S + + Q + + ++EK + + + + + K+ Q + Sbjct: 1082 TNEVAQSGSETKE----TQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSE 1137 Query: 480 ESRQALEDAQTNNNLEKAAELQYGTIPQLEKELRELEDNFQDEQGEDTDRMIREVVTDEE 539 + E A+ N+ E Q + + ++ ++T + + VT+ Sbjct: 1138 TVQPQAEPARENDPTVNIKEPQ-------SQTNTTAD---TEQPAKETSSNVEQPVTEST 1187 Query: 540 IGDIVSQWTGIPVSKLVETEREK 562 + + P + T + Sbjct: 1188 TVNTGNSVVENPENTTPATTQPT 1210

SAI8T7_1007980

SAI8T7_1008030

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1007980	4	13	-1.065845	Similar to methyltransferase
SAI8T7_1007990	4	14	-1.048854	Phosphopantetheine adenylyltransferase
SAI8T7_1008000	5	12	-1.600947	UPF0348 protein NWMN_0989
SAI8T7_1008010	4	12	-1.702031	Putative uncharacterized protein
SAI8T7_1008020	4	13	-1.754714	Iron-regulated surface determinant protein B
SAI8T7_1008030	2	13	-2.757987	Iron-regulated surface determinant protein A

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1007990	LPSBIOSNTHSS	219	1e-76	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 219 bits (560), Expect = 1e-76 Identities = 77/155 (49%), Positives = 112/155 (72%) Query: 5 IAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKEGTFSLEERMDLIEQSVKHLP 64 A+ PGSFDPIT+GHLDIIER FD+++V VL+N K+ FS++ER++ I +++ HLP Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61 Query: 65 NVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELRLTSMNKKLNNEIETLYMMSSTN 124 N +V F GL V+Y Q A I+RGLR +SDFE EL++ + NK L +++ET+++ +ST Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121 Query: 125 YSFISSSIVKEVAAYRADISEFVPPYVEKALKKKF 159 YSF+SSS+VKEVA + ++ FVP +V AL +F Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHVAAALYDQF 156
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008020	IGASERPTASE	36	6e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 35.8 bits (82), Expect = 6e-04 Identities = 37/194 (19%), Positives = 71/194 (36%), Gaps = 15/194 (7%) Query: 447 RIVDKEAFTKANTDKSNKKEQQDNSAKKEA---------TPATPSKPTPSPVEKESQKQD 497 + VD T N +++ N+ + PATPS+ T + E Q+ Sbjct: 990 QTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESK 1049 Query: 498 SQKDDNKQLPSVEKENDASSESGKDKTPATKPT------KGEVESSSTTPTKVVSTTQNV 551 + + + + +N ++ K A T E + + TT TK +T + Sbjct: 1050 TVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKE 1109 Query: 552 AKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLP 611 K + KT + TS S + + S +Q + T + +Q N Sbjct: 1110 EKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTE 1169 Query: 612 QTGEESNKDMTLPL 625 Q +E++ ++ P+ Sbjct: 1170 QPAKETSSNVEQPV 1183 Score = 30.0 bits (67), Expect = 0.035 Identities = 27/156 (17%), Positives = 45/156 (28%), Gaps = 5/156 (3%) Query: 37 EAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAPET-KPVANAVSVSNKEVEAPTSETKE 95 A EE TE + V S + ++ ET +P A ++ V +++ Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQT 1162 Query: 96 AKEVKEVKAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEM 155 + KET + + T N + NP + P Sbjct: 1163 NTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVE----NPENTTPATTQPTVNSESSNK 1218 Query: 156 KKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQS 191 K + +V+PA D L S Sbjct: 1219 PKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTS 1254
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008030	IGASERPTASE	34	0.001	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/132 (20%), Positives = 44/132 (33%), Gaps = 4/132 (3%) Query: 184 ADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTT 243 + A+ + P PA P TE + K + + +V + Sbjct: 1015 EEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKS 1074 Query: 244 KDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNET 303 + TQT + AQ+ E + QT + V K+Q+ KVT + Sbjct: 1075 NVKANTQTN---EVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS-QVS 1130 Query: 304 PKQASKAKELPK 315 PKQ P+ Sbjct: 1131 PKQEQSETVQPQ 1142

SAI8T7_1008880

SAI8T7_1008990

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1008880	2	11	-1.833700	Ribonuclease HII
SAI8T7_1008890	2	10	-1.230184	Succinyl-CoA ligase [ADP-forming] subunit beta
SAI8T7_1008900	2	11	-1.134523	Succinyl-CoA ligase [ADP-forming] subunit alpha
SAI8T7_1008910	2	10	-1.850900	Probable cell wall hydrolase lytN
SAI8T7_1008920	1	10	-0.804860	FmhC protein
SAI8T7_1008930	1	9	0.808711	Similar to DNA processing Smf protein
SAI8T7_1008940	3	9	1.069846	DNA topoisomerase
SAI8T7_1008950	3	12	0.974671	Methylenetetrahydrofolate--tRNA-(uracil-5-)-
SAI8T7_1008960	5	16	1.044862	Tyrosine recombinase XerC
SAI8T7_1008970	4	18	1.666387	ATP-dependent protease subunit HslV
SAI8T7_1008980	5	19	1.173932	ATP-dependent protease ATPase subunit HslU
SAI8T7_1008990	4	20	0.500853	GTP-sensing transcriptional pleiotropic

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008910	GPOSANCHOR	36	2e-04	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 36.2 bits (83), Expect = 2e-04 Identities = 15/90 (16%), Positives = 32/90 (35%), Gaps = 4/90 (4%) Query: 12 MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKSKDFTRGYEQNVFAKSE 71 M K + YS+RK+ G S+++ AL + ++ + + K + Sbjct: 1 MTKNNTNRHYSLRKLKTGTASVAV----ALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQ 56 Query: 72 LNANKNTTKDKIKNEGAVKTSDTSLKLDNK 101 A+K ++ S + L + Sbjct: 57 ERADKFEIENNTLKLKNSDLSFNNKALKDH 86

SAI8T7_1009620

SAI8T7_1009680

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1009620	2	11	-2.274390	Putative uncharacterized protein
SAI8T7_1009630	3	12	-2.123750	LexA repressor
SAI8T7_1009640	3	11	-1.632073	Transketolase
SAI8T7_1009650	2	10	-1.472199	Putative uncharacterized protein
SAI8T7_1009660	3	10	-1.408928	Possible exonuclease SbcD
SAI8T7_1009670	3	11	-1.103097	Putative Nuclease sbcCD subunit C
SAI8T7_1009680	2	13	1.032397	Putative uncharacterized protein MW1234

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1009640	CHANLCOLICIN	30	0.041	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 29.7 bits (66), Expect = 0.041 Identities = 18/58 (31%), Positives = 26/58 (44%), Gaps = 5/58 (8%) Query: 296 QNTMLKRANEDESQ-----WNSLLEKYAETYPELAEEFKLAISGKLPKNYKDELPRFE 348 QN +L +D + +L EKY E Y ++A+E GK N + L FE Sbjct: 337 QNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFE 394
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1009670	FbpA_PF05833	34	0.005	Fibronectin-binding protein
>FbpA_PF05833#Fibronectin-binding protein Length = 577 Score = 33.7 bits (77), Expect = 0.005 Identities = 39/249 (15%), Positives = 83/249 (33%), Gaps = 4/249 (1%) Query: 241 LQARSKEILAFVNESKETAIKEYEIIEKKTLENNILKDNINQLNKNKIDFVQLKEQQPEI 300 I F E+ + + + +L N ID ++ Sbjct: 174 FDFSYDMIENFTKENSLQLNDNIFSKIFTGVSKTLSSEICFRLKNNSIDLSLSNLKEIVE 233 Query: 301 DEIEAKLKLLQDITNLLNYIENREKIETKIAN--SKKDISKTNNKILNLDCDKRNIDKEK 358 + ++ + Y +N + N SK+D K + + K+K Sbjct: 234 VCKDLFKEIQSNKFEFNCYTKNNSFVGFYCLNLMSKEDYKKIQYDSSSKLLENFYYAKDK 293 Query: 359 K--MLEENGDLIESKTSFIDKTRVLFNDINKYQQSYLNIECLITEGEQLGDELNNLIKGL 416 + ++ DL + + I++ +N + + + GE L + L KGL Sbjct: 294 SDRLKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCEDKDIFKLYGELLTANIYALKKGL 353 Query: 417 EKVEDSIGNNESDYEKIIELNNAITNINNEINIIKENEKAKAELDKLLGSKQELENQINE 476 +E + +E+ I L+ T N + K+ K K + + E ++N Sbjct: 354 SHIELANYYSENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAANEQLLQNEEELNY 413 Query: 477 ETTIMKNLE 485 +++ N+ Sbjct: 414 LYSVLTNIN 422

SAI8T7_1010260

SAI8T7_1010360

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1010260	2	13	-1.688552	UDP-N-acetylglucosamine--N-acetylmuramyl-
SAI8T7_1010270	3	13	-0.882417	Putative uncharacterized protein
SAI8T7_1010280	1	10	-0.988746	C-terminal processing peptidase family protein
SAI8T7_1010290	0	12	-0.000506	Glucose-specific phosphotransferase enzyme IIA
SAI8T7_1010300	-1	10	-0.523722	Peptide methionine sulfoxide reductase MsrA 2
SAI8T7_1010310	9	11	1.874837	DegV domain-containing protein SACOL1460
SAI8T7_1010320	9	10	1.841688	Dihydrofolate reductase
SAI8T7_1010330	9	10	1.808400	Putative Thymidylate synthase
SAI8T7_1010340	9	10	1.872246	Conserved virulence factor C
SAI8T7_1010350	9	11	1.889078	Putative uncharacterized protein
SAI8T7_1010360	9	10	1.907854	Putative uncharacterized protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1010270	SACTRNSFRASE	32	5e-04	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 32.2 bits (73), Expect = 5e-04 Identities = 33/140 (23%), Positives = 54/140 (38%), Gaps = 19/140 (13%) Query: 30 EQWDDQYPLLEHFEEDIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVI 89 +Q++D + + EE+ +LY LE + G I + N G +I Sbjct: 48 KQYEDDDMDVSYVEEEGKAAFLYYLE--NNCIGRIKIRS-------------NWNGYALI 92 Query: 90 HRLTGSKEY--KGAATELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQ 147 + +K+Y KG T L + I+ K ++ +T +N A +AK F Sbjct: 93 EDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAVD 152 Query: 148 LMEYP--PYDKGEPFYAYYK 165 M Y P + YYK Sbjct: 153 TMLYSNFPTANEIAIFWYYK 172
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1010360	GPOSANCHOR	45	1e-05	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 45.4 bits (107), Expect = 1e-05 Identities = 49/323 (15%), Positives = 96/323 (29%), Gaps = 9/323 (2%) Query: 2582 TKVRAAQTKIDQAKALLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDN------- 2634 T +A Q L + +E + N L+ + + + D Sbjct: 37 TNEVSAVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSN 96 Query: 2635 YNAKKREAETEITAAQRVIDNGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQA 2694 K R+ + ++ I +A + N TA + L A+ AL Sbjct: 97 AKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAAR 156 Query: 2695 VQQLNRTGTTTGKKPASITAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNV 2754 L + + +A ++ A ++ L + + ++ + + + + Sbjct: 157 KADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTL 216 Query: 2755 NRVNERLTQAINQLVPLADNSALRTAKTKLDEEINKSVTTDGMTQSSIQTYENAKRAGQT 2814 L L T +I ++ E A Sbjct: 217 EAEKAALAARKADL--EKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMN 274 Query: 2815 ETTNAQNVINNGDATDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQ 2874 +T I +A + AEK +E + L L DL + AK QL+ + + Sbjct: 275 FSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQK 334 Query: 2875 PTSTTGMTSASVAAFNDKLSAAR 2897 ++ AS + L A+R Sbjct: 335 LEEQNKISEASRQSLRRDLDASR 357 Score = 40.8 bits (95), Expect = 3e-04 Identities = 56/339 (16%), Positives = 103/339 (30%), Gaps = 24/339 (7%) Query: 2732 SANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVPLAD-----NSALRTAKTKLDE 2786 + + T+++VQ N L + L N L + E Sbjct: 40 VSAVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKE 99 Query: 2787 EINKSVTTDGMTQSSIQTYENAKRAGQTETTNAQNVINNGDATDQQIAAEKTKVEEKYNS 2846 ++ K+ + S IQ E K + A N A + + AEK + + Sbjct: 100 KLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKAD 159 Query: 2847 LKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASVAAFNDKLSAARTKIQEIDRV 2906 L++A+ G L+ + A A L A + Sbjct: 160 LEKALEGAMNFSTADSAKIKTLEAEKAA-------LEARQAELEKALEGAM------NFS 206 Query: 2907 LASHPDVATIRQNVTAANAAKTALDQARNGLTVDKAPLENAKNQLQHSIDTQTSTTGMTQ 2966 A + T+ A A K L++A G L+ + + Sbjct: 207 TADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELE 266 Query: 2967 DSINAYNAKLTAARNKVQQINQVLAGSPTVDQINTNTSAANQAKSDLDHARQALTPDKAP 3026 ++ TA K++ + + + L+ RQ+L D Sbjct: 267 KALEGAMNFSTADSAKIKTLEA------EKAALEAEKADLEHQSQVLNANRQSLRRDLDA 320 Query: 3027 LQNAKTQLEQSINQPTDTTGMTTASLNAYNQKLQAARQK 3065 + AK QLE + + ++ AS + + L A+R+ Sbjct: 321 SREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREA 359

SAI8T7_1011450

SAI8T7_1011560

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1011450	2	15	0.576102	Putative uncharacterized protein MW1528
SAI8T7_1011460	1	13	-0.402074	Ribosomal RNA small subunit methyltransferase E
SAI8T7_1011470	2	12	-0.369411	Ribosomal protein L11 methyltransferase
SAI8T7_1011480	1	12	-0.341166	Chaperone protein DnaJ
SAI8T7_1011490	1	12	-1.532314	Chaperone protein DnaK
SAI8T7_1011500	0	12	-3.441310	Protein grpE
SAI8T7_1011510	0	13	-3.319726	Heat-inducible transcription repressor HrcA
SAI8T7_1011520	0	13	-3.149398	Oxygen-independent coproporphyrinogen oxidase
SAI8T7_1011530	-1	13	-3.490806	Elongation factor 4
SAI8T7_1011540	1	16	-4.591237	Putative uncharacterized protein
SAI8T7_1011550	1	16	-3.636942	Similar to late competence protein ComEC
SAI8T7_1011560	2	18	-2.224068	Possible competence protein ComEB

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011490	SHAPEPROTEIN	163	2e-47	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 163 bits (415), Expect = 2e-47 Identities = 79/363 (21%), Positives = 145/363 (39%), Gaps = 58/363 (15%) Query: 10 SKIIGIDLGTTNSCVTVLEG----DEPKVIQ-NPEGSRTTPSVVAFKNGETQVGEVAKRQ 64 S + IDLGT N+ + V +EP V+ + + + SV A VG AK+ Sbjct: 10 SNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA-------VGHDAKQM 62 Query: 65 AITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAESYLGEKVDKAVITV 124 P + +I+ K + + +++ ++ + + + + ++ V Sbjct: 63 LGRTPGNIAAIR-----PMKDGVIADFFVTEKMLQHFIKQVHSNS---FMRPSPRVLVCV 114 Query: 125 PAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDKDEKVLVFDLGGGTFDV 184 P ER+A +++ + AG +I EP AAA+ GL + +V D+GGGT +V Sbjct: 115 PVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGL-PVSEATGSMVVDIGGGTTEV 173 Query: 185 SILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKENGVDLSQDKMALQRLKDAA 244 +++ L V + ++GGD FD+ II+Y+ + G + A Sbjct: 174 AVISLNGVV-----YSSSVRIGGDRFDEAIINYVRRNYGSLIG-------------EATA 215 Query: 245 EKAKKDLS----GVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSL------IRRT 294 E+ K ++ G +I + + E P +N + E L + L + Sbjct: 216 ERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLN-SNEILEALQEPLTGIVSAVMVA 274 Query: 295 MEPTRQAMKDAGLTNSDIDE--VILVGGSTRIPAVQEAVKKEIGKEPNKGVNPDEVVAMG 352 +E + SDI E ++L GG + + + +E G +P VA G Sbjct: 275 LEQCPPELA------SDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARG 328 Query: 353 AAI 355 Sbjct: 329 GGK 331
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011530	TCRTETOQM	184	2e-52	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 184 bits (468), Expect = 2e-52 Identities = 106/439 (24%), Positives = 184/439 (41%), Gaps = 89/439 (20%) Query: 12 NIRNFSIIAHIDHGKSTLADRILEN---TKSVETRDMQDQLLDSMDLERERGITIKLNAV 68 I N ++AH+D GK+TL + +L N + + D D+ LER+RGITI+ Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 69 RLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLA 128 ++E N +IDTPGH+DF EV RSL+ +GAIL++ A G++AQT + Sbjct: 62 SFQWENTKVN-----IIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHAL 116 Query: 129 LDNELELLPVINKIDLPAAEPERV--------------KQEIE--------DMIGLDQDD 166 + + INKID + V KQ++E + +Q D Sbjct: 117 RKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWD 176 Query: 167 VVLA---------------------------------------SAKSNIGIEEILEKIVE 187 V+ SAK+NIGI+ ++E I Sbjct: 177 TVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITN 236 Query: 188 VVPAPDGDPEAPLKALIFDSEYDPYRGVISSIRIVDGVVKAGDKIRMMATGKEFEVTEVG 247 + ++ L +F EY R ++ IR+ GV+ D +R+ K ++TE Sbjct: 237 KFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITE-- 293 Query: 248 INTPKQ---LPVDELTVGDVGYIIASIKNVDDSRVGDTITLASRPASEPLQGYKKMNPMV 304 + T +D+ G++ + ++ +GDT L P E ++ P++ Sbjct: 294 MYTSINGELCKIDKAYSGEIVILQNEFLKLNSV-LGDTKLL---PQRERIENPL---PLL 346 Query: 305 YCGLFPIDNKNYNDLREALEKLQLNDASLEFE--PESSQALGFGYRTGFLGMLHMEIIQE 362 + P + L +AL ++ +D L + + + + FLG + ME+ Sbjct: 347 QTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEII-----LSFLGKVQMEVTCA 401 Query: 363 RIEREFGIELIATAPSVIY 381 ++ ++ +E+ P+VIY Sbjct: 402 LLQEKYHVEIEIKEPTVIY 420 Score = 35.6 bits (82), Expect = 6e-04 Identities = 12/75 (16%), Positives = 28/75 (37%), Gaps = 2/75 (2%) Query: 408 IFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVNIVYELPLAEVVFDFFDQ 467 + EPY+ + P +Y+ + ++ ++ V + E+P + ++ Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNE-VILSGEIPARC-IQEYRSD 592 Query: 468 LKSNTKGYASFDYEF 482 L T G + E Sbjct: 593 LTFFTNGRSVCLTEL 607

SAI8T7_1011950

SAI8T7_1012020

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1011950	2	13	-0.874566	GTPase obg
SAI8T7_1011960	5	22	-1.353435	Similar to cell shape determinant mreD
SAI8T7_1011970	8	24	-0.391426	Rod shape-determining protein MreC
SAI8T7_1011980	9	26	-0.283450	Putative uncharacterized protein
SAI8T7_1011990	9	25	-0.056321	Putative uncharacterized protein
SAI8T7_1012000	9	24	-0.771946	rRNA adenine N-6-methyltransferase
SAI8T7_1012010	6	20	-0.756404	Streptomycin=3''-adenylyltransferase
SAI8T7_1012020	2	10	-0.602333	Transposase B from transposon Tn554

SAI8T7_1013300

SAI8T7_1013410

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1013300	2	10	-2.682918	Glutamyl endopeptidase
SAI8T7_1013310	3	12	-3.902729	Serine protease splA
SAI8T7_1013320	4	13	-4.308101	Putative Probable beta-lactamase
SAI8T7_1013330	4	14	-4.573290	Putative uncharacterized protein
SAI8T7_1013340	5	16	-4.607500	Leukotoxin, LukD
SAI8T7_1013350	7	18	-5.810955	Leukotoxin S-subunit
SAI8T7_1013360	9	20	-7.177831	Putative uncharacterized protein
SAI8T7_1013370	10	20	-6.561952	Putative uncharacterized protein
SAI8T7_1013380	8	19	-7.051920	Protein of hypothetical function DUF1828
SAI8T7_1013390	7	19	-6.235395	Enterotoxin type G
SAI8T7_1013400	4	15	-4.069391	Enterotoxin SeN
SAI8T7_1013410	2	12	-2.749384	Extracellular enterotoxin type I

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013300	V8PROTEASE	182	4e-58	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 182 bits (462), Expect = 4e-58 Identities = 65/230 (28%), Positives = 108/230 (46%), Gaps = 29/230 (12%) Query: 43 EVQQTAKA-----ENNVTKIQDTNIFPYTGVVAFKS--------ATGFVVGKNTILTNKH 89 ++Q A N+ +I DT Y V + A+G VVGK+T+LTNKH Sbjct: 60 PLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKH 119 Query: 90 V-SKNYKVGDRITAHP---NSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGF 145 V + + A P N D G ++ ++I Y G+ D+++++ + Sbjct: 120 VVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNK----- 174 Query: 146 NFNDNVTPFKYAAGA--KAGERIKVIGYPHPYKNKYVLYESTGPVMSVEGSSIVYSAHTE 203 + + V P + A + + I V GYP K ++ES G + ++G ++ Y T Sbjct: 175 HIGEVVKPATMSNNAETQVNQNITVTGYPGD-KPVATMWESKGKITYLKGEAMQYDLSTT 233 Query: 204 SGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENID 253 GNSGSPV N NE++GIH+ V N+ N V+ ++ F+ +NI+ Sbjct: 234 GGNSGSPVFNEKNEVIGIHWGG-VPNEFNG---AVFINENVRNFLKQNIE 279
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013310	V8PROTEASE	138	1e-41	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 138 bits (349), Expect = 1e-41 Identities = 63/212 (29%), Positives = 100/212 (47%), Gaps = 18/212 (8%) Query: 36 EKNVKEITDATKAPYNSVVAFA--------GGTGVVVGKNTIVTNKHIAKSNDIFKNRVA 87 + +ITD T Y V +GVVVGK+T++TNKH+ + + + Sbjct: 73 NNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALK 132 Query: 88 AHYS---SKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGA-- 142 A S G + + I +Y G+ DLAIV + + + + V + A Sbjct: 133 AFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVK-FSPNEQNKHIGEVVKPATMSNNAET 191 Query: 143 KAKDRISVIGYPKGAQTKYKMFESTGTINHISGTFIEFDAYAQPCNSGSPVLNSKHELIG 202 + I+V GYP G + M+ES G I ++ G +++D NSGSPV N K+E+IG Sbjct: 192 QVNQNITVTGYP-GDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIG 250 Query: 203 ILYAGSGKDESEKNFGVYFTPQLKEFIQNNIE 234 I + G +E N V+ ++ F++ NIE Sbjct: 251 IHWGGVP---NEFNGAVFINENVRNFLKQNIE 279
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013340	BICOMPNTOXIN	396	e-141	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 396 bits (1020), Expect = e-141 Identities = 96/329 (29%), Positives = 177/329 (53%), Gaps = 24/329 (7%) Query: 1 MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQ 60 M K++ ++++ S+ L + ++ A+ + I + K T ++K ++Q Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 61 ILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQ-FYWGGKYNVSVSSESNDA 119 + F+F+KDK Y+KD L+LK G I+S N K N+ + W +YN+ + + + Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTN-DKY 119 Query: 120 VNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTI 179 V++++Y PKN+ E V QTLGY+ GG+ + L G NGS ++S++I+Y Q++Y + + Sbjct: 120 VSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGG--NGSFNYSKSISYTQQNYVSEV 177 Query: 180 DRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQM 239 +++ N KS+ WGV+A+ + ++LF+G + S + F+P ++ Sbjct: 178 EQQ-NSKSVLWGVKANSFAT-------ESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSEL 229 Query: 240 PLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD---------RYTNQWNRLHWIGN 290 P L + FNP FI+ +SH++ + S+ ++TY R MD Y N + H + N Sbjct: 230 PPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHN 289 Query: 291 NYKNQNTVTFTSTYEVDWQNHTVKLIGTD 319 + N+N +T YEV+W+ H +K+ G + Sbjct: 290 AFVNRN---YTVKYEVNWKTHEIKVKGQN 315
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013350	BICOMPNTOXIN	433	e-156	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 433 bits (1116), Expect = e-156 Identities = 214/318 (67%), Positives = 256/318 (80%), Gaps = 10/318 (3%) Query: 1 MFKKKMLAATLSVGLIAPLASPIQE-SRANTNIENIGDGA--EVIKRTEDVSSKKWGVTQ 57 M K K+L TLSV L+APLA+P+ E ++A + E+IG G+ E+IKRTED +S KWGVTQ Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 58 NVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIWPFQYNIGLTTKDP 117 N+QFDFVKDKKYNKDALI+KMQGFI+SRT++ + K + + K M WPFQYNIGL T D Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNH--VKAMRWPFQYNIGLKTNDK 118 Query: 118 NVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSKTISYTQKSYVSEVD 177 VSLINYLPKNKIE+T+V QTLGYNIGGNFQSAPS+GGNGSFNYSK+ISYTQ++YVSEV+ Sbjct: 119 YVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVE 178 Query: 178 KQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREYFAPDNQLPPLVQSGF 237 +QNSKSV WGVKAN F T G+KSA D LFV + R+YF PD++LPPLVQSGF Sbjct: 179 QQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPH-SKDPRDYFVPDSELPPLVQSGF 237 Query: 238 NPSFITTLSHEKGSSDTSEFEISYGRNLDITYA----TLFPRTGIYAERKHNAFVNRNFV 293 NPSFI T+SHEKGSSDTSEFEI+YGRN+D+T+A T + + + R HNAFVNRN+ Sbjct: 238 NPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYT 297 Query: 294 VRYEVNWKTHEIKVKGHN 311 V+YEVNWKTHEIKVKG N Sbjct: 298 VKYEVNWKTHEIKVKGQN 315
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013390	BACTRLTOXIN	195	4e-64	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 195 bits (497), Expect = 4e-64 Identities = 109/261 (41%), Positives = 155/261 (59%), Gaps = 11/261 (4%) Query: 4 LSTVIIILILEIVFHNMN-YVNAQPDPKLDELNKVSDYKNNKGTMGNVMNLYTSPPVEGR 62 +S VI+I L +V N +QPDP D+L+K S++ GTMGN+ LY V Sbjct: 7 ISRVILIFALILVISTPNVLAESQPDPMPDDLHKSSEFT---GTMGNMKYLYDDHYVSAT 63 Query: 63 GVINSRQFLSHDLIFPI---EYKSYNEVKTELENTELANNYKDKKVDIFGVPYFYTCIIP 119 V + +FL+HDLI+ I + K+Y++VKTEL N +LA YKD+ VD++G Y+ C Sbjct: 64 KVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNCYFS 123 Query: 120 KSEPDINQNFGGCCMYGGLTF---NSSENERDKLITVQVTIDNRQSLGFTITTNKNMVTI 176 + G CMYGG+T N +N + + V+V + R ++ F + T+K VT Sbjct: 124 SKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENKRNTISFEVQTDKKSVTA 183 Query: 177 QELDYKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWFDLFPKKELVPFVPYKFLN 236 QELD KAR++L +K LYEF+ S +E+GYIKF E N +FW+D+ P F K+L Sbjct: 184 QELDIKARNFLINKKNLYEFNSSPYETGYIKFIENNGNTFWYDMMPAPGDK-FDQSKYLM 242 Query: 237 IYGDNKVVDSKSIKMEVFLNT 257 +Y DNK VDSKS+K+EV L T Sbjct: 243 MYNDNKTVDSKSVKIEVHLTT 263
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013400	BACTRLTOXIN	155	9e-49	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 155 bits (394), Expect = 9e-49 Identities = 76/265 (28%), Positives = 124/265 (46%), Gaps = 21/265 (7%) Query: 2 RLFYIAAIII-TLLCLINNNYVNAEV----DKKDLKKKSDLDSSKLFNLTSYYTDITWQL 56 RLF I+I L+ +I+ V AE DL K S+ + + N+ Y D + Sbjct: 4 RLFISRVILIFALILVISTPNVLAESQPDPMPDDLHKSSEF-TGTMGNMKYLYDDH--YV 60 Query: 57 DESNKISTDQLLNNTIILKNIDISVLKTSSLKVEFNSSDLANQFKGKNIDIYGLYFGNKC 116 + S D+ L + +I D + +K E + DLA ++K + +D+YG + C Sbjct: 61 SATKVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNC 120 Query: 117 -------VGLTEEKTSCLYGGVTIHDGNQLDEEKV--IGVNVFKDGVQQEGFVIKTKKAK 167 VG +C+YGG+T H+GN D + + V V+++ F ++T K Sbjct: 121 YFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENKRNTISFEVQTDKKS 180 Query: 168 VTVQELDTKVRFKLENLYKIYNKDTGNIQKGCIFFHSHNHQDQSFYYDLYNVKGSVG--A 225 VT QELD K R L N +Y ++ + G I F +N +F+YD+ G + Sbjct: 181 VTAQELDIKARNFLINKKNLYEFNSSPYETGYIKFIENN--GNTFWYDMMPAPGDKFDQS 238 Query: 226 EFFQFYSDNRTVSSSNYHIDVFLYK 250 ++ Y+DN+TV S + I+V L Sbjct: 239 KYLMMYNDNKTVDSKSVKIEVHLTT 263
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013410	BACTRLTOXIN	108	2e-30	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 108 bits (270), Expect = 2e-30 Identities = 54/227 (23%), Positives = 98/227 (43%), Gaps = 37/227 (16%) Query: 30 VGNLRNFYTKHDYIDLKGVTDKNLPIANQLEFS------TGTNDLISESNNWDEISKFKG 83 +GN++ Y H K + +A+ L ++ + + +E N D K+K Sbjct: 48 MGNMKYLYDDHYVSATKVKSVDKF-LAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKD 106 Query: 84 KKLDIFGIDY-------------NGPCKSKYMYGGATL-SGQYLNSARKIPINLWVNGKH 129 + +D++G +Y MYGG T G + ++ + + V Sbjct: 107 EVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENK 166 Query: 130 KTISTDKIATNKKLVTAQEIDVKLRRYLQEEYNIYGHNNTGKGKEYGYKSKFYSGFNNGK 189 + + ++ T+KK VTAQE+D+K R +L + N+Y N+ + G Sbjct: 167 RNTISFEVQTDKKSVTAQELDIKARNFLINKKNLY-EFNSSP-------------YETGY 212 Query: 190 VLFHLNNEKSFSYDLF-YTGDGLPVS-FLKIYEDNKIIESEKFHLDV 234 + F NN +F YD+ GD S +L +Y DNK ++S+ ++V Sbjct: 213 IKFIENNGNTFWYDMMPAPGDKFDQSKYLMMYNDNKTVDSKSVKIEV 259

SAI8T7_1014590

SAI8T7_1014650

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1014590	0	11	-3.607005	Peptidase C45 acyl-coenzyme
SAI8T7_1014600	0	12	-4.763581	Putative uncharacterized protein
SAI8T7_1014610	1	11	-5.254071	Putative uncharacterized protein
SAI8T7_1014620	2	12	-4.736594	Putative uncharacterized protein
SAI8T7_1014630	0	11	-3.452830	Thioredoxin
SAI8T7_1014640	-1	11	-4.279029	Putative uncharacterized protein
SAI8T7_1014650	-1	12	-3.714843	Similar to ABC transporter (ATP-binding

SAI8T7_1014740

SAI8T7_1014820

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1014740	2	13	1.602720	Phage tail protein
SAI8T7_1014750	2	12	1.239175	Bacteriophage tail length tape measure protein
SAI8T7_1014760	1	16	0.105008	Putative uncharacterized protein
SAI8T7_1014770	2	18	0.263446	Phage capsid protein
SAI8T7_1014780	2	21	0.028924	PhiN315 scaffolding protein-like protein
SAI8T7_1014790	4	23	0.262874	Putative uncharacterized protein
SAI8T7_1014800	2	23	0.485456	Phage terminase large subunit
SAI8T7_1014810	4	27	1.196516	Putative uncharacterized protein
SAI8T7_1014820	4	26	0.530546	Replicative DnaB-like helicase family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1014750	TYPE4SSCAGA	36	0.001	Type IV secretion system CagA exotoxin signature.
>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature. Length = 1147 Score = 36.2 bits (83), Expect = 0.001 Identities = 90/447 (20%), Positives = 172/447 (38%), Gaps = 69/447 (15%) Query: 15 DSANLNRSLTEIKRNFRTLNSDLKLTGN--NFKYTEKSTDSYKQRIKELDGTIAGYKKNI 72 D + N+ L NF +D K TGN K +K + ++ + L+ + K + Sbjct: 576 DFLSSNKELVGKTLNFNKAVADAKNTGNYDEVKKAQKDLEKSLRKREHLEKEVE---KKL 632 Query: 73 DDLAKQYDKVSQEQGENSTK--------------------AQNLRQEYNKQANELNFLEK 112 + + +K+ + NS K AQNL+ + +++L + K Sbjct: 633 ESKSGNKNKMEAKAQANSQKDEIFALINKEANRDARAIAYAQNLKGIKRELSDKLENVNK 692 Query: 113 ELEKTTAEFEEFKKAQVEAQRMAESGWGKTSKIFESMGPKLTKMGDGLKSIGKGMMIGVT 172 L+ F+EFK + + K + +++ + +G + I K Sbjct: 693 NLKDFDKSFDEFKNGK-------NKDFSKAEETLKALKGSVKDLGINPEWISK------- 738 Query: 173 APVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVYGN--FPADAETVGG 230 V + AA + +K VTQA L+NS KDV N + + Sbjct: 739 --VENLNAALNEFKNGKNKDFSKVTQAK-------SDLENSVKDVIINQKVTDKVDNLNQ 789 Query: 231 VLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMGDAGIEASEYQSVLD 290 + F+ +E A FS + Q + + +A ++ YQSV + Sbjct: 790 AVSVAKATGDFSR--VEQALADLKNFSKEQLAQQAQKNESL-----NARKKSEIYQSVKN 842 Query: 291 MVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQWEKSGVNTEIAFSGLKK 350 V +G+S A +++K + ++ E+ + F+ +G+ E ++ + K Sbjct: 843 GVNGTLVGNGLS-QAEATTLSKNFSDIKK---ELNAKLGNFNNNNNNGLKNEPIYAKVNK 898 Query: 351 AISNWGKAGKNP--REEFKKTLAEIERTPDIASATSLAIEA--FGAKAGPDLADAIKGGR 406 + + + P + KK A+I+R IAS + +A F K + D K G Sbjct: 899 KKAGQAASLEEPIYAQVAKKVNAKIDRLNQIASGLGVVGQAAGFPLKRHDKVDDLSKVGL 958 Query: 407 FSYQEFLKTIEDSQGTVNQTFKDSESG 433 QE + I++ +NQ ++++G Sbjct: 959 SRNQELAQKIDN----LNQAVSEAKAG 981

SAI8T7_1015800

SAI8T7_1016100

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1015800	3	20	-0.101199	Cation-efflux system membrane protein homolog
SAI8T7_1015810	8	25	-0.318930	Putative Lytic regulatory protein truncated with
SAI8T7_1015820	8	23	-0.630034	Putative uncharacterized protein
SAI8T7_1015830	7	20	-0.754410	rRNA adenine N-6-methyltransferase
SAI8T7_1015840	6	18	-0.538295	Streptomycin=3''-adenylyltransferase
SAI8T7_1015850	1	11	-0.425604	Transposase B from transposon Tn554
SAI8T7_1015860	-1	13	-0.042467	Transposase A from transposon Tn554
SAI8T7_1015870	-1	11	0.145975	Putative Lytic regulatory protein truncated with
SAI8T7_1015880	-1	11	0.614231	Putative uncharacterized protein
SAI8T7_1015890	7	13	1.140842	Similar to ATP-binding protein homolog
SAI8T7_1015900	7	14	1.143645	Glucosamine--fructose-6-phosphate
SAI8T7_1015910	7	12	0.822547	PTS system mannitol-specific EIICB component
SAI8T7_1015920	7	11	0.616605	PRD domain protein
SAI8T7_1015930	9	15	0.545200	Mannitol-1-phosphate=5-dehydrogenase
SAI8T7_1015940	8	15	0.821496	FmtB protein
SAI8T7_1015950	2	13	-0.319174	Phosphoglucosamine mutase
SAI8T7_1015960	3	15	-0.550028	Putative uncharacterized protein SA1966
SAI8T7_1015970	3	16	-0.612308	Conserved hypothetoical protein
SAI8T7_1015980	4	16	-1.376195	Arginase
SAI8T7_1016060	5	14	-1.280895	******ATP-binding protein Mrp-like protein
SAI8T7_1016070	3	12	-2.391229	Similar to multidrug resistance protein
SAI8T7_1016080	2	13	-2.274026	Drug resistance transporter, EmrB/QacA
SAI8T7_1016090	1	12	-1.537205	Putative Multidrug resistance efflux pump sepA
SAI8T7_1016100	2	11	-0.897888	SA1972 protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015890	PF05272	29	0.017	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 29.3 bits (65), Expect = 0.017 Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 8/58 (13%) Query: 32 ILYGLNGAGKTTLLNILNAYEPATTGGVNLFGKMPGKVGYSAETVRQHIGFVSHSLLE 89 +L G G GK+TL+N L G++ F +G ++ Q G V++ L E Sbjct: 600 VLEGTGGIGKSTLINTL--------VGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSE 649
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015940	IGASERPTASE	45	7e-06	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 44.7 bits (105), Expect = 7e-06 Identities = 57/316 (18%), Positives = 103/316 (32%), Gaps = 20/316 (6%) Query: 2122 PQANNNSSADASTNSPTMDNDVTSKPEVESTNNG---TTDKPVTETDNATPAESTTNN-- 2176 P+ + +TN T +N P V S N + PV ATP+E+T Sbjct: 983 PEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAE 1042 Query: 2177 ----NSTTTATNENAPTGSTATAPTTASTEAASSADSKDNASVNDSKQNAEVNNSAESQS 2232 S T NE T +TA A ++ + V S + + E++ Sbjct: 1043 NSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKE 1102 Query: 2233 TNGKVAQPKS--ENKAKAEKDGRDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEEST 2290 T + K+ E + E S E + P A+ N P+ K+ + T Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQT 1162 Query: 2291 TNQTDAGQLKSETNVASNEADKSPSKADT----EVSNKPSTSASSEAKDKMTSTNVSQKD 2346 D Q ET+ + + +T + + +T A+++ S+N + Sbjct: 1163 NTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNR 1222 Query: 2347 DTATADTNDTQKSVGPVANNKAKDMQTNDTQKSVGSAANNKATQNDGANASPATVSNG-S 2405 + + ++N + S N + A A ++ G + Sbjct: 1223 HRRSVRSVPHNVEPATTSSNDRS----TVALCDLTSTNTNAVLSDARAKAQFVALNVGKA 1278 Query: 2406 HSMHQDMLNVTKPEEN 2421 S H L + + Sbjct: 1279 VSQHISQLEMNNEGQY 1294 Score = 42.0 bits (98), Expect = 4e-05 Identities = 47/318 (14%), Positives = 104/318 (32%), Gaps = 9/318 (2%) Query: 1073 NNGSTTEEKEAAKQQVQTEKTAADAAIDAAHSNVEVEAAKNAEIAKI-EAIQPATTTKDN 1131 NG +++ QT T + ++V + N EIA++ EA P Sbjct: 974 VNGRYDLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATP 1033 Query: 1132 AKQAIATKANERKTAIAQTQDITAEEIAAANADVDNAVTQANSNIEAANSQNDVDQAKTT 1191 ++ N ++ ++T + ++ A +A SN++A N+V Q+ + Sbjct: 1034 SETTETVAENSKQE--SKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE 1091 Query: 1192 GETSIDQVTPTVNKKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTENGKANQ 1251 + T T K TA E + ++ Q P T + + + Sbjct: 1092 TKE-----TQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPA 1146 Query: 1252 AISAATTNAQVDEAKANAEAAINAVTPKVVKKQAAKDEIDQLQATQTNVINNDQNATNEE 1311 + T N + +++ N A + T +V+ N +N T Sbjct: 1147 RENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPAT 1206 Query: 1312 KEAAIQQLATAVTDAKNNITAATDDNGVDTAKDAGKNSIQSTQPATAVKSNAKNEVDQAV 1371 + + ++ ++ + + + V+ A + + + +N + A Sbjct: 1207 TQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDR-STVALCDLTSTNTNAVLSDAR 1265 Query: 1372 TTQNQAIDNTTGATTEEK 1389 N A ++ Sbjct: 1266 AKAQFVALNVGKAVSQHI 1283 Score = 33.9 bits (77), Expect = 0.010 Identities = 61/312 (19%), Positives = 105/312 (33%), Gaps = 18/312 (5%) Query: 1021 DIDNATANTDVDNAKTTNEATIAAITPDANVKPAAKQAIADKVQAQETAIDANNGSTTEE 1080 D+ N TTN T I D P+ + IA +A S T E Sbjct: 979 DLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTE 1038 Query: 1081 K--EAAKQQVQTEKTAADAAIDAAHSNVEVEAAKNAEIAKIEAIQPATTTKDNAKQAIAT 1138 E +KQ+ +T + A + N EV + + A T + Q+ + Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNV-------KANTQTNEVAQSGSE 1091 Query: 1139 KANERKTAIAQTQDITAEEIAAANADVDNAVTQANSNIEAANSQNDVDQAKTTGETSIDQ 1198 + T +T + EE A + V + S + Q++ Q + D Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREND- 1150 Query: 1199 VTPTVN-KKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTENGKANQAISAAT 1257 PTVN K+ ++ TA +E + + E + + N + AT Sbjct: 1151 --PTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENT--TPAT 1206 Query: 1258 TNAQVDEAKANAEAAINAVTPKVVKKQ---AAKDEIDQLQATQTNVINNDQNATNEEKEA 1314 T V+ +N + + + V A D+ ++ + + NA + A Sbjct: 1207 TQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARA 1266 Query: 1315 AIQQLATAVTDA 1326 Q +A V A Sbjct: 1267 KAQFVALNVGKA 1278 Score = 33.5 bits (76), Expect = 0.013 Identities = 45/305 (14%), Positives = 93/305 (30%), Gaps = 14/305 (4%) Query: 804 KNEEIFKIENITDSTQTKMDAYKEVRQAATARKAQNATVSNATDEEVAEANAAVDAAQTE 863 E K E+ T + + A++A++ +N EVA++ + QT Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTT 1098 Query: 864 GLHDIQVVKSQQEVADTKAKVLDKINAIQTQAKVKPAADTEVENAYNTRKQEIQNSNAST 923 + V+ +++ A + + ++ + +Q K V+ ++ N Sbjct: 1099 ETKETATVEKEEK-AKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKE 1157 Query: 924 TEEKEAAYTELDAKKQEARTNLDAANTNSDVTTAKDNGIAAINQVQAATTKKSDAKAEIA 983 + + + + +E +N++ T T N + + T + +E + Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVT-ESTTVNTGNSVVENPENTTPATTQPTVNSESS 1216 Query: 984 QKASERKTAIEAMNDSTTEEQQAAKDKVDQAVVTANADIDNATANTDVDNAKTTNEATIA 1043 K R + E V + N A AK A Sbjct: 1217 NKPKNR-HRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVA--- 1272 Query: 1044 AITPDANVKPAAKQAIADKV---QAQETAIDANNGSTTEEKEAAKQQVQTEKTAADAAID 1100 NV A Q I+ + Q +N + ++ ++ T D Sbjct: 1273 -----LNVGKAVSQHISQLEMNNEGQYNVWVSNTSMNKNYSSSQYRRFSSKSTQTQLGWD 1327 Query: 1101 AAHSN 1105 SN Sbjct: 1328 QTISN 1332 Score = 32.3 bits (73), Expect = 0.034 Identities = 32/210 (15%), Positives = 63/210 (30%), Gaps = 4/210 (1%) Query: 34 TTASAAEQNQPAQNQPAQPADANTQPNANAGAQANPAAQPANQGGQANPAGGAAQPAGQG 93 T + +Q P Q QP A + +P Q N QPA + Sbjct: 1116 TEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKET 1175 Query: 94 NQADPNNAAQAQPGNQ--AAPANQAGQGNNQATPNNNATPANQTQPANAPA-AAQPAAPV 150 + ++ N + N P N+ +N+ + + + + P Sbjct: 1176 SSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVE 1235 Query: 151 AANAQTQDPNASNTGE-GSINTTLTFDDPAISTDENRQDPTVTVTDKVNGYSLINNGKIG 209 A + D + + S NT D + V+ ++ + N G+ Sbjct: 1236 PATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQHISQLEMNNEGQYN 1295 Query: 210 FVNSELRRSDMFDKNNPQNYQAKGNVAALG 239 S + + + + + +K LG Sbjct: 1296 VWVSNTSMNKNYSSSQYRRFSSKSTQTQLG 1325
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016070	TCRTETB	50	1e-09	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 50.3 bits (120), Expect = 1e-09 Identities = 37/147 (25%), Positives = 69/147 (46%), Gaps = 2/147 (1%) Query: 1 MIIMMSMVGPALLIPLYVQNSLSLSALLSGLVIM-PGAIINGIMSVFTGKFYDKYGPRPL 59 II ++ G ++P +++ LS G VI+ PG + I G D+ GP + Sbjct: 266 GIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYV 325 Query: 60 IYTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEISH 119 + G T L+++ + FL TS+ I++ + S I+T +SL+ +E Sbjct: 326 LNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL-SFTKTVISTIVSSSLKQQEAGA 384 Query: 120 GTAIMNFGRVMAGSLGTALMVTLMSFG 146 G +++NF ++ G A++ L+S Sbjct: 385 GMSLLNFTSFLSEGTGIAIVGGLLSIP 411
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016080	TCRTETB	91	3e-23	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 91.5 bits (227), Expect = 3e-23 Identities = 58/257 (22%), Positives = 120/257 (46%), Gaps = 12/257 (4%) Query: 8 TTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELNINESTSQWLVTGFMLVNGVMIP 67 + R N I+ + I +F ++LN+ +LN +LP I + N +++ W+ T FML + Sbjct: 8 SNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTA 67 Query: 68 LTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPN-FGVLMLARVIQAMGAGVLMPLMQFT 126 + L D++ + L L + GS++ + + F +L++AR IQ GA L+ Sbjct: 68 VYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVV 127 Query: 127 LFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLIIDQASWRVPFIIIVGIALVAFVFGL 186 + KE+RG A GL G ++ +GP + G+I W +++++ + + V L Sbjct: 128 VARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHW--SYLLLIPMITIITVPFL 185 Query: 187 VSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAFSSAGDLGFTSPIVIGALIISMVIIYLF 246 + + D + ++ ++G + FT+ I LI+S++ +F Sbjct: 186 MKLLKKEVRIKGHFDIKGIILMSVGIVFFML---------FTTSYSISFLIVSVLSFLIF 236 Query: 247 IRRQFNITNVLLNLKVF 263 ++ +T+ ++ + Sbjct: 237 VKHIRKVTDPFVDPGLG 253
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016100	TCRTETB	103	5e-26	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 103 bits (257), Expect = 5e-26 Identities = 91/405 (22%), Positives = 175/405 (43%), Gaps = 14/405 (3%) Query: 9 VIALILIMFMSAIESSIISLALPTIKQDLNA-GNLISLIFTAYFIALVIANPIVGELLSR 67 +I L ++ F S + +++++LP I D N + + TA+ + I + G+L + Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75 Query: 68 FKIIYVAIAGLLLFSIGSFMCGLS-TNFTMLIISRVIQGFGSGVLMSLSQIVPKLAFEIP 126 I + + G+++ GS + + + F++LI++R IQG G+ +L +V Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135 Query: 127 LRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAIILVIWTFHFPEE 186 R K G++GS+ + +GP +GG I HW + + IP +I II V + ++ Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAH--YIHWSYLLLIP--MITIITVPFLMKLLKK 191 Query: 187 ETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVAMCLFKVEKHVSSP 246 E K FD KG+ L V I M + + I++++ + K + V+ P Sbjct: 192 EVRIKGHFDIKGIILMSVGIVFFMLFTTSY-----SISFLIVSVLSFLIFVKHIRKVTDP 246 Query: 247 FLPVVEF-NRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPLQSG-LVIFPLSVAW 304 F+ N + + + + GF +P +++ LS + G ++IFP +++ Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSV 306 Query: 305 ITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKL-PVLIAFVLILAGLSFGYIY 363 I + + + + + T L VS + SF ++ + +++ + Sbjct: 307 IIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTK 366 Query: 364 TKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAIQ 408 T S IV + MS T L G I+G L +I Sbjct: 367 TVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIP 411

SAI8T7_1018420

SAI8T7_1018580

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1018420	2	15	-1.493621	Putative Transposase
SAI8T7_1018430	4	15	-1.514951	Putative uncharacterized protein
SAI8T7_1018440	6	16	-1.434940	Uncharacterized oxidoreductase SAV2478
SAI8T7_1018450	9	17	-2.469082	Putative lipoprotein
SAI8T7_1018460	8	17	-3.723550	Putative uncharacterized protein
SAI8T7_1018470	10	19	-4.780336	Putative lipoprotein
SAI8T7_1018480	4	15	-3.709489	Putative lipoprotein
SAI8T7_1018490	3	15	-3.530426	Putative uncharacterized protein
SAI8T7_1018500	3	14	-2.796412	Similar to putative helicase
SAI8T7_1018510	4	14	-2.230657	Type III restriction enzyme, res subunit
SAI8T7_1018520	3	14	-1.624735	Putative uncharacterized protein
SAI8T7_1018530	2	11	-0.850073	Phosphomannomutase
SAI8T7_1018540	4	12	0.951006	Putative uncharacterized protein
SAI8T7_1018550	3	12	1.753085	Surface protein G
SAI8T7_1018560	0	9	1.927933	Cell wall anchor protein
SAI8T7_1018570	-1	10	2.277169	Putative SarU
SAI8T7_1018580	-1	10	3.157516	UTP--glucose-1-phosphate uridylyltransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1018440	DHBDHDRGNASE	102	2e-28	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 102 bits (254), Expect = 2e-28 Identities = 66/245 (26%), Positives = 118/245 (48%), Gaps = 23/245 (9%) Query: 7 KIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQNVANQLAQDS--VKVVPTDVTN 64 KIA +TGA GIGEA+A L +GA + N +KL+ V + L ++ + P DV + Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 65 KEEVDELMKIAQQTFGGLDIVINSAGQMLSSKITDYQVDEWDSMIDVNIKGTLYTAQAAL 124 +DE+ ++ G +DI++N AG + I +EW++ VN G +++ Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 125 PTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAVHTITQGLEKELAKTGVKVTSISPG 184 M+++ SG ++ + S S Y+++KAA T+ L ELA+ ++ +SPG Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 185 MVDTAITAAYNPSD--------------------RKKLDPQDIAEAVLYALT-QPKHVNV 223 +T + + + +K P DIA+AVL+ ++ Q H+ + Sbjct: 189 STETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITM 248 Query: 224 NEITV 228 + + V Sbjct: 249 HNLCV 253
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1018550	V8PROTEASE	34	2e-04	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 34.2 bits (78), Expect = 2e-04 Identities = 14/30 (46%), Positives = 18/30 (60%) Query: 93 EKPKDPKGPENPEKPSRPTHPSGPVNPNNP 122 P +P P NP+ P+ P P+ P NPNNP Sbjct: 290 NNPDNPDNPNNPDNPNNPDEPNNPDNPNNP 319 Score = 32.7 bits (74), Expect = 7e-04 Identities = 13/30 (43%), Positives = 18/30 (60%) Query: 93 EKPKDPKGPENPEKPSRPTHPSGPVNPNNP 122 + P +P P+NP P P +P P NP+NP Sbjct: 293 DNPDNPNNPDNPNNPDEPNNPDNPNNPDNP 322 Score = 32.3 bits (73), Expect = 0.001 Identities = 13/30 (43%), Positives = 19/30 (63%) Query: 93 EKPKDPKGPENPEKPSRPTHPSGPVNPNNP 122 ++P +P P+NP P P +P P NP+NP Sbjct: 287 DQPNNPDNPDNPNNPDNPNNPDEPNNPDNP 316 Score = 30.7 bits (69), Expect = 0.003 Identities = 12/29 (41%), Positives = 20/29 (68%) Query: 93 EKPKDPKGPENPEKPSRPTHPSGPVNPNN 121 + P +P P NP++P+ P +P+ P NP+N Sbjct: 296 DNPNNPDNPNNPDEPNNPDNPNNPDNPDN 324 Score = 29.6 bits (66), Expect = 0.007 Identities = 17/46 (36%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Query: 98 PKGPENPEKPSRPTHPSGPVNPNNPGLSKDRAKP-NGPVHSMDKND 142 P P+NP+ P+ P +P+ P PNNP + P NG ++ D D Sbjct: 289 PNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD 334 Score = 27.7 bits (61), Expect = 0.027 Identities = 13/33 (39%), Positives = 17/33 (51%) Query: 102 ENPEKPSRPTHPSGPVNPNNPGLSKDRAKPNGP 134 + P P P +P+ P NPNNP + PN P Sbjct: 287 DQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNP 319
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1018560	GPOSANCHOR	30	0.014	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 30.4 bits (68), Expect = 0.014 Identities = 17/108 (15%), Positives = 33/108 (30%), Gaps = 1/108 (0%) Query: 16 FLSNKLNKYSIRKFTVGTASILIG-SLMYLGTQQEAEAAENNIENPTTLKDNVQSKEVKI 74 +N YS+RK GTAS+ + +++ G T + Sbjct: 2 TKNNTNRHYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADK 61 Query: 75 EEVTNKDTAPQGVEAKSEVTSNKDTIEHEASVKAEDISKKEDTPKEVA 122 E+ N + + + KD + + K K ++ Sbjct: 62 FEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLS 109

SAI8T7_1019280

SAI8T7_1019380

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1019280	2	17	0.372307	Putative X-Pro dipeptidyl-peptidase (S15) family
SAI8T7_1019290	7	24	-0.467340	LPXTG-motif cell wall anchor domain
SAI8T7_1019300	10	26	-0.493443	Putative uncharacterized protein
SAI8T7_1019310	9	26	-0.107757	Putative uncharacterized protein
SAI8T7_1019320	8	23	0.176867	rRNA adenine N-6-methyltransferase
SAI8T7_1019330	5	20	0.744114	Streptomycin=3''-adenylyltransferase
SAI8T7_1019340	2	18	0.915916	Transposase B from transposon Tn554
SAI8T7_1019350	-2	16	1.403280	Transposase A from transposon Tn554
SAI8T7_1019360	-1	12	2.160097	Truncated-SA protein
SAI8T7_1019370	0	10	2.955664	Pantothenate synthetase
SAI8T7_1019380	1	10	3.311112	3-methyl-2-oxobutanoate

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019290	SUBTILISIN	28	0.025	Subtilisin serine protease family (S8) signature.
>SUBTILISIN#Subtilisin serine protease family (S8) signature. Length = 326 Score = 28.3 bits (63), Expect = 0.025 Identities = 7/36 (19%), Positives = 14/36 (38%) Query: 24 ANAENNKPEGVNENSNLIPVRQPDANYPGPVSDIAR 59 A N GV ++L+ ++ + G I + Sbjct: 96 ATENENGVVGVAPEADLLIIKVLNKQGSGQYDWIIQ 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019300	V8PROTEASE	66	3e-15	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 65.8 bits (160), Expect = 3e-15 Identities = 33/121 (27%), Positives = 58/121 (47%), Gaps = 8/121 (6%) Query: 1 MNAPTKEDIAIIKLNS-----NLGNKTGYLTLNTHISK--GENIEISGFPGDKSDNRQYK 53 + D+AI+K + ++G T++ + +NI ++G+PGDK ++ Sbjct: 154 TKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWE 213 Query: 54 GKGKLESFDENEMYYTVDTFSGQSGSAIRDSKNNIIGVHAYG-RYNHNSGVRINDLKLDY 112 KGK+ M Y + T G SGS + + KN +IG+H G N V IN+ ++ Sbjct: 214 SKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNF 273 Query: 113 I 113 + Sbjct: 274 L 274
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019360	V8PROTEASE	49	2e-09	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 48.8 bits (116), Expect = 2e-09 Identities = 13/91 (14%), Positives = 28/91 (30%), Gaps = 2/91 (2%) Query: 72 VFGKDQRTVVNNILQRPYKQTVLLNMTFSNNRVYKGTGTMIGKDIVLTAAHNVYSKDDKG 131 + + R + + Y + + + +G ++GKD +LT H V Sbjct: 70 ILPNNDRHQITDTTNGHYAPVTYIQVEAPTGT-FIASGVVVGKDTLLTNKH-VVDATHGD 127 Query: 132 WAKKIDVYAGVNGQTYTIGKAFSHKFFVSKT 162 + +N Y G + + Sbjct: 128 PHALKAFPSAINQDNYPNGGFTAEQITKYSG 158

SAI8T7_1019840

SAI8T7_1019890

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1019840	7	13	2.228436	Protein translocase subunit SecA 2
SAI8T7_1019850	9	14	2.631603	Putative uncharacterized protein
SAI8T7_1019860	9	14	2.789941	Putative uncharacterized protein
SAI8T7_1019870	11	15	2.787090	Putative uncharacterized protein
SAI8T7_1019880	11	16	2.981833	Accessory Sec system protein translocase subunit
SAI8T7_1019890	12	17	3.725961	Serine-rich adhesin for platelets

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019840	SECA	648	0.0	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 648 bits (1672), Expect = 0.0 Identities = 282/823 (34%), Positives = 441/823 (53%), Gaps = 68/823 (8%) Query: 2 KRINTWSDEVKSYSDDALKQKTIEFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPK 61 IN E++ SD+ LK KT EF+ RL G + L+ L+PEA+AV REAS RV GM Sbjct: 26 NIINAMEPEMEKLSDEELKGKTAEFRARLEKG-EVLENLIPEAFAVVREASKRVFGMRHF 84 Query: 62 EVQLIGAIVLHEGNIAEMQTGEGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQ 121 +VQL+G +VL+E IAEM+TGEGKTLTAT+P YLNAL+GKG +++T NDYLA+RD E + Sbjct: 85 DVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNR 144 Query: 122 PLYEWLGLTASLGFVDIVDYEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKF 181 PL+E+LGLT V I KR Y DI Y TN GFDYL DN+A S E + Sbjct: 145 PLFEFLGLT-----VGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERV 199 Query: 182 LPQLNYGIIDEVDSIILDAAQTPLVISGAPRLQSNLFHIVKEFVDTLIE----------- 230 +L+Y ++DEVDSI++D A+TPL+ISG S ++ V + + LI Sbjct: 200 QRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQG 259 Query: 231 DVHFKMKKTKKEIWLLNQGIEAAQSYFNV-------EDLYSEQAMVLVRNINLALRAQYL 283 + HF + + +++ L +G+ + E LYS ++L+ ++ ALRA L Sbjct: 260 EGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHAL 319 Query: 284 FESNVDYFVYNGDIVLIDRITGRMLPGTKLQAGLHQAIEAKEGMEVSTDKSVMATITFQN 343 F +VDY V +G+++++D TGR + G + GLHQA+EAKEG+++ + +A+ITFQN Sbjct: 320 FTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQN 379 Query: 344 LFKLFESFSGMTATGKLGESEFFDLYSKIVVQAPTDKAIQRIDEPDKVFRSVDEKNIAMI 403 F+L+E +GMT T EF +Y V PT++ + R D PD V+ + EK A+I Sbjct: 380 YFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAII 439 Query: 404 HDIVELHETGRPVLLITRTAEAAEYFSKVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGS 463 DI E G+PVL+ T + E +E S L + I +N+L A+ A EA ++A+AG + Sbjct: 440 EDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAA 499 Query: 464 MTVATSMAGRGTDIKLG-----------------------------EGVEALGGLAVIIH 494 +T+AT+MAGRGTDI LG + V GGL +I Sbjct: 500 VTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGT 559 Query: 495 EHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYSLDAQRLSQ 554 E E+ R+D QLRGRSGRQGD GSS Y+S++D L++ ++ ++ + + + Sbjct: 560 ERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIE 619 Query: 555 SNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNRVLEIDDAENR 614 + + AQR E + R+ E++ + QR +Y +RN +L++ D Sbjct: 620 HPWVTKAIA----NAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSET 675 Query: 615 DFKALAKDVFEMFVNEE---KVLTKSRVVEYIYQNLSFQFNKDVACVNFKDKQAVVT--- 668 ++ +DVF+ ++ + L + + + + L F+ D+ + DK+ + Sbjct: 676 -INSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEET 734 Query: 669 ---FLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKASVNQRQ 725 +L Q + ++ + A F + V L+ +DS W E + + L+ ++ R Sbjct: 735 LRERILAQSIEVYQ-RKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRG 793 Query: 726 NGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKE 768 Q++ EY R + F M ++K ++ + + + +E Sbjct: 794 YAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEE 836
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019880	SECYTRNLCASE	128	2e-35	Preprotein translocase SecY subunit signature.
>SECYTRNLCASE#Preprotein translocase SecY subunit signature. Length = 437 Score = 128 bits (324), Expect = 2e-35 Identities = 93/440 (21%), Positives = 181/440 (41%), Gaps = 52/440 (11%) Query: 4 LLQQYEYKIIYKRMLYTCFILFIYILGTNISI--VSYNDMQ------VKHESFFKIAISN 55 + + + K++L+T I+ +Y +GT+I I V Y ++Q ++ F + Sbjct: 5 FARAFRTPDLRKKLLFTLAIIVVYRVGTHIPIPGVDYKNVQQCVREASGNQGLFGLVNMF 64 Query: 56 MGGDVNTLNIFTLGLVPWLTSMIILMLISYRNMDKYMKQTSLEKHYKE------------ 103 GG + + IF LG++P++T+ IIL L++ + LE KE Sbjct: 65 SGGALLQITIFALGIMPYITASIILQLLT-------VVIPRLEALKKEGQAGTAKITQYT 117 Query: 104 RILTLILSVIQSYFVIHEYVSKERVHQDN-------------IYLTILILVTGTILLVWL 150 R LT+ L+++Q ++ S + + ++ + GT +++WL Sbjct: 118 RYLTVALAILQGTGLVATARSAPLFGRCSVGGQIVPDQSIFTTITMVICMTAGTCVVMWL 177 Query: 151 ADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYI------DASHIVIALLIILVIITLFILL 204 + + GI M I+M I + + I I +I + +I + +++ Sbjct: 178 GELITDRGIGNGMSILMFISIAATFPSALWAIKKQGTLAGGWIEFGTVIAVGLIMVALVV 237 Query: 205 FIELVEVRIPYI----DLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFIL 260 F+E + RIP + S +Y+ KVN AG I ++ + S F Sbjct: 238 FVEQAQRRIPVQYAKRMIGRRSYGGTSTYIPLKVNQAGVIPVIFASSLLYIPALVAQFAG 297 Query: 261 SMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGV 320 + + D P+ I Y ++ + +F N ++ + + K G + G+ Sbjct: 298 GNSGWKSWVEQNLTKGDHPIYIVTYFLLIVFFAFFYVAISFNPEEVADNMKKYGGFIPGI 357 Query: 321 KPGKDTERYLNYQARRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFS-VQLIVLVYIS 379 + G+ T YL+Y R+ W GS + +I +P L S F ++++V + Sbjct: 358 RAGRPTAEYLSYVLNRITWPGSLYLGLIALVP-TMALVGFGASQNFPFGGTSILIIVGVG 416 Query: 380 INIAETIRTYLYFDKYKPFL 399 + + I + L Y+ FL Sbjct: 417 LETVKQIESQLQQRNYEGFL 436
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019890	ICENUCLEATIN	55	3e-09	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 55.1 bits (132), Expect = 3e-09 Identities = 237/1070 (22%), Positives = 425/1070 (39%), Gaps = 12/1070 (1%) Query: 1098 SDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSVSD 1157 + + ++ + S S + + T +T S ST ++ + Sbjct: 106 LHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQTIEIATYG 165 Query: 1158 STSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQSASAFLSESLSE 1217 ST T +S I+ ST + + + S + ++ST + S ES Sbjct: 166 STLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQM 225 Query: 1218 STSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKSE 1277 + ST + S + S T+ S+ + ST + S+ T+ ST T + Sbjct: 226 AGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 285 Query: 1278 SVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSL 1337 S T+ ST T+ ++S+ ++ S T+ +S + ST + S + ST Sbjct: 286 SDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGT 345 Query: 1338 SGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQ 1397 +G S + S+ + DS+ + S T+ S T+ S T+ + S + Sbjct: 346 AGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYG 405 Query: 1398 SGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQ 1457 S + S + ST T++ S T+ Y S T+ +S+ + S T+ S+ Sbjct: 406 STQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLT 465 Query: 1458 SGSTSTSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSA 1517 +G ST + GS+ + S ST+ ES+ + S + S + ST + + Sbjct: 466 AGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNE 525 Query: 1518 SASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSE 1577 S + STS + + S+ + S ++ S+ + ST + ST Sbjct: 526 SDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGT 585 Query: 1578 SGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSD 1637 +GS S + ST T+ S T+ S + S T+ STS + + S + Sbjct: 586 AGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYG 645 Query: 1638 SQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVM 1697 S + S T+ ST + SD T+ S ST+G+ S I+ ST T+ S + Sbjct: 646 STQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 705 Query: 1698 SASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESS 1757 + S + S S S S + +DS ++G S + S T+ S + Sbjct: 706 AGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQ 765 Query: 1758 SLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTST 1817 S+ + S S + +DSS ++ S +++ S + S T+ S T+G STST Sbjct: 766 SVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTST 825 Query: 1818 SLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQ 1877 + + S ++ S + S T+ S + S + STS + DS ++ Sbjct: 826 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYG 885 Query: 1878 SMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSD 1937 S + S + S+ T+ D + S S + S GS T++ ST Sbjct: 886 STQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLM 945 Query: 1938 SMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSIST 1997 + GS + S + GSTS++ S+ + S + QST T+ GS T+ + Sbjct: 946 AGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQTAEHS 1005 Query: 1998 SMSMSASTSSSQSTSVSTSLSTSDSISDST----------SISISGSQSTVESESTSDST 2047 S + S++ + + S+ ++ S S S ISG +S + + S Sbjct: 1006 STLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGYGSSLI 1065 Query: 2048 SISDSESLSTSDSDSTSTSTSDSTSGSTSTSIS--ESLSTSGSGSTSVSDSTSMSESDST 2105 S S + S+ ++ S +G ST I+ S+ +G GS+ + S S + Sbjct: 1066 SGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGAD 1125 Query: 2106 SVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSESLSTSMSGSQSI 2155 SV M+ ++ + +DS + S L+ ++S T+ S +G+ I Sbjct: 1126 SVQMAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDCI 1175 Score = 53.2 bits (127), Expect = 1e-08 Identities = 176/773 (22%), Positives = 305/773 (39%), Gaps = 2/773 (0%) Query: 1408 SASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSTSTSASL 1467 + + +E + S + + T D+T S ST + + + Sbjct: 106 LHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQTIEIATYG 165 Query: 1468 SGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASESDSSST 1527 S SQ I+ S T+ +ST ++ ST +G+ ST + S + +SS Sbjct: 166 STLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQM 225 Query: 1528 SLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSE 1587 + ST M+ S+ + S + S+ + ST + S T+ GST + Sbjct: 226 AGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 285 Query: 1588 SDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTST 1647 SD T+ S + + S+ +G ST T+ +S T+ ST SD + ST T Sbjct: 286 SDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGT 345 Query: 1648 STSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSM 1707 + S + S + DS+ + GS + SD T+ S + S + Sbjct: 346 AGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYG 405 Query: 1708 SESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSD 1767 S ES + S + GS + GS + + S+ +G S Sbjct: 406 STQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLT 465 Query: 1768 SVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSE 1827 + S ++ S S S + S + GST T+ GS T+ S + +E Sbjct: 466 AGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNE 525 Query: 1828 STSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTST 1887 S ++ S S + + S + GS + S+ T+ S + S +G ST T Sbjct: 526 SDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGT 585 Query: 1888 SVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGSVSVST 1947 + SDS + S + S+ + ST + S + S ST+ + S ++ Sbjct: 586 AGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYG 645 Query: 1948 STSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSS 2007 ST + S T+ S+ T+ S + S ST+ + S ++ ST + S + Sbjct: 646 STQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 705 Query: 2008 SQSTSVSTSLSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTST 2067 + S T+ SD S S S +G+ S++ + S T+ S + S T+ Sbjct: 706 AGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQ 765 Query: 2068 SDSTS--GSTSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESV 2125 S T+ GSTST+ ++S +G GST + S+ + S +Q++SD T+ S S Sbjct: 766 SVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTST 825 Query: 2126 STSTSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPS 2178 + + S+ ++ ST T+ S +G S + S + S S + + S Sbjct: 826 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDS 878 Score = 53.2 bits (127), Expect = 1e-08 Identities = 233/1050 (22%), Positives = 411/1050 (39%), Gaps = 2/1050 (0%) Query: 759 TSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKSTSVSLSDSVSASKSLSTSES 818 TS + A ++ + S + D+ S S +++ Sbjct: 99 TSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQT 158 Query: 819 NSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNSNSTEKSESLSTSTSDSLRTSTS 878 +++ ST QS + GS + S I+ ST + + ST + T T+ Sbjct: 159 IEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTA 218 Query: 879 LSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDSTSNAISTSTSLSESASTSDSISIS 938 +S M+ GS S +G ST + DS+ A ST + S+ + S Sbjct: 219 GEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 278 Query: 939 NSIANSQSASTSKSDSQSTSISLSTSDSKSMSTSESLSDSTSTSGSVSGSLSIAASQSVS 998 A S T+ S T+ + S+ + ST + +ST T+G GS A S Sbjct: 279 TQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGY--GSTQTAQKGSDL 336 Query: 999 TSTSDSMSTSEIVSDSISTSGSLSASDSKSMSVSSSMSTSQSGSTSESLSDSQSTSDSDS 1058 T+ S T+ S I+ GS + S + ST + S+ + ST + + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 1059 KSLSLSTSQSGSTSTSTSTSASVRTSESQSTSGSMSASQSDSMSISTSFSDSTSDSKSAS 1118 S ++ S T+ ST + S + GS + S + S + S Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 456 Query: 1119 TASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSVSDSTSLSTSESDSISESTSTSDS 1178 TA +S + ST + S + S T+ S + S + ST T+ Sbjct: 457 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGY 516 Query: 1179 ISEAISASESTSISLSESNSTSDSESQSASAFLSESLSESTSESTSESVSSSTSESTSLS 1238 S + +ES I+ S ST+ + S + + S + S T+ S+ T+ S Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDL 576 Query: 1239 DSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKSESVSTSLSMSTSTSLSNSTSLS 1298 + S T+ S S+ +G S T++ S T+ + S + S+ T+ S ST+ + Sbjct: 577 TAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGA 636 Query: 1299 TSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDS 1358 S + S + S+ T+ ST + S T+ GSTS + +DS+ + S Sbjct: 637 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQ 696 Query: 1359 TSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTS 1418 T+ S+ + GST T+ S S S S + + + S ++ +S+ T+ Sbjct: 697 TAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGY 756 Query: 1419 ESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQS 1478 S + + S ST+ + S + S T+ S T+ S ++ S Sbjct: 757 GSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDL 816 Query: 1479 ISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQ 1538 + S ST+ + S+ ++ ST +G S T+ S ++ +S T+ STS + Sbjct: 817 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 876 Query: 1539 SSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDS 1598 S + S + S T+ ST + S T+ GSTS + ES + S Sbjct: 877 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQ 936 Query: 1599 QSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLS 1658 ++ +ST +G S+ T+ S T+ STSM S + ST T+ S T+ Sbjct: 937 TASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGY 996 Query: 1659 DSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVS 1718 S + ST + GS + + + S + S+ S + S ++ SV Sbjct: 997 GSTQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVL 1056 Query: 1719 ESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLS 1778 + S S S+ + GS +++ + ES+ ++G++SM + S ++ Sbjct: 1057 TAGYGSSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGY 1116 Query: 1779 VSTSLRSSESVSESDSLSDSKSTSGSTSTS 1808 ST + ++SV + + + ST T+ Sbjct: 1117 RSTLISGADSVQMAGERGKLIAGADSTQTA 1146 Score = 52.8 bits (126), Expect = 2e-08 Identities = 229/1007 (22%), Positives = 395/1007 (39%), Gaps = 10/1007 (0%) Query: 1163 TSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQSASAFLSESLSESTSES 1222 TS I + + +E + S ++ ES S +++ Sbjct: 99 TSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQT 158 Query: 1223 TSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKSESVSTS 1282 + ST T S + GST T+ +ST + ST T+ ++ST S T+ Sbjct: 159 IEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTA 218 Query: 1283 LSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSLSGSTS 1342 S+ + ST SD T+ S + S+ + S + S+ +G S Sbjct: 219 GEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 278 Query: 1343 ESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQSGVDS 1402 + S + ST + + S +G ST T+ S T+ S + S + + Sbjct: 279 TQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTA 338 Query: 1403 NSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSTS 1462 S + S+ + S T+ S T+ ST T+ SD T+ GST Sbjct: 339 GYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTA------GYGSTG 392 Query: 1463 TSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASES 1522 T+ + S + S + S T+ ST + S +G ST T+ +S+ + Sbjct: 393 TAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGY 452 Query: 1523 DSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTS 1582 S+ T+ DS+ + S +Q S + STST+ S++ ++ ST +G S Sbjct: 453 GSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSL--IAGYGSTQTAGYGS 510 Query: 1583 ESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMS 1642 T+ ST T+ ++S + S S + + S+ + ST ++ S+ T+ S + Sbjct: 511 TLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTA 570 Query: 1643 LSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASIS 1702 S T+ ST + S S + S T+ S + ST T+ S + + S Sbjct: 571 REGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGS 630 Query: 1703 DSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGS 1762 S + ++S + S + +S +G S + S T+ S S + + S + Sbjct: 631 TSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIA 690 Query: 1763 QSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGS 1822 S + +S + S ++++ S+ S S ST+G+ S+ +G ST T+ S Sbjct: 691 GYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHS 750 Query: 1823 ESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGS 1882 + S + S T+ S S +G+ S + ST + S + S + Sbjct: 751 SLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTA 810 Query: 1883 ESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGS 1942 + S + S+ST+ DS I+ S + +S +G ST T+ SD +G Sbjct: 811 QERSDLTTGYGSTSTAG--ADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGY 868 Query: 1943 VSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMS 2002 S ST+ S I+G S + S T+ S +Q S +G STS + S Sbjct: 869 GSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSL 928 Query: 2003 ASTSSSQSTSVSTSLSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDS 2062 + S T+ S + S T+ S + S S + S + ST + Sbjct: 929 IAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGY 988 Query: 2063 TSTSTSDSTSGSTSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDS 2122 ST T+ S T+ S + GS +T+ +DS+ ++ S+ S + + S Sbjct: 989 QSTLTAGYGSTQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTL 1048 Query: 2123 ESVSTSTSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTST 2169 S S T+ S S S T+ GS I+ S+ ++G ST Sbjct: 1049 ISGLRSVLTAGYGSSLISGRRSSLTAGYGSNQIASHRSSLIAGPEST 1095 Score = 52.1 bits (124), Expect = 3e-08 Identities = 193/856 (22%), Positives = 350/856 (40%), Gaps = 14/856 (1%) Query: 1329 DSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTS 1388 S + ++ + + + S + + + + T +T S ST + Sbjct: 97 TKTSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPT 156 Query: 1389 LSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDS 1448 ++ + S + SQ + ST T+ S + Y S T+ ++ST + S Sbjct: 157 QTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQ 216 Query: 1449 TSISKSTSQSGSTSTSASLSGSESESDSQSISTSASEST--SESASTSLSDSTSTSNSGS 1506 T+ +S+ +G ST + GS+ + S T+ +S+ + ST + S+ +G Sbjct: 217 TAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGY 276 Query: 1507 ASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTI 1566 ST T+ S + S+ T+ +DS+ + S + S + ST T+ + S + Sbjct: 277 GSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDL 336 Query: 1567 ASLSTSVSTSESGST------SESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDS 1620 + S T+ S+ S T+ DS+ T+ S T++ S + ST T+ + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 1621 RSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVS 1680 S+ + S +T+ +S + ST T+ S + S T+ S+ +G S Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 456 Query: 1681 ISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGS 1740 + DS+ T+ S + SD + S + + S + S +G S +G Sbjct: 457 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGY 516 Query: 1741 LSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKS 1800 S T+ +S+ ++ S S + + S ++ S+ + S+ ++ S + S Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDL 576 Query: 1801 TSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTS 1860 T+G ST T+GS S+ + GS + S + S T+ S +G S S + + Sbjct: 577 TAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGA 636 Query: 1861 LST------SDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDS 1914 S+ S + S+ ++ S + S + STS + DS I+ S Sbjct: 637 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQ 696 Query: 1915 MSTSDSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLS 1974 + +S +G ST T+ SD SG S ST+ + S I+G S +S S+ T+ Sbjct: 697 TAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGY 756 Query: 1975 DSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTSLSTSDSISDSTSISISGS 2034 S ++ S +G STS + + S + S T+ S+ T+ S T+ S Sbjct: 757 GSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDL 816 Query: 2035 QSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGSTSTSISESLSTSGSGSTSVS 2094 + S ST+ + S + ST + S T+ S T+ S+ + GS ST+ Sbjct: 817 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 876 Query: 2095 DSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSESLSTSMSGSQS 2154 DS+ ++ ST + + S + S T+ S ST+ ES + GS Sbjct: 877 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQ 936 Query: 2155 ISDSTSTSMSGSTSTS 2170 + ST M+G S+ Sbjct: 937 TASFKSTLMAGYGSSQ 952 Score = 51.3 bits (122), Expect = 5e-08 Identities = 200/886 (22%), Positives = 350/886 (39%), Gaps = 6/886 (0%) Query: 797 KSTSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS 856 ++ + + SA + + ++ V+ + + S V+S + D + Sbjct: 89 RAEVLHVGTKTSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATI 148 Query: 857 NSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDSTS 916 S + + + T + S ++ GS + ST I+G ST + +DST Sbjct: 149 ESGSTQPTQTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTL 208 Query: 917 NAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTSESLS 976 A ST + S+ + S S T+ S T+ S+ + ST + Sbjct: 209 VAGYGSTQTAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGE 268 Query: 977 DSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMSVSSSMS 1036 DS+ T+G S + S + S + ++ + S + +S + S Sbjct: 269 DSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQ 328 Query: 1037 TSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQSTSGSMSAS 1096 T+Q GS + S T+ DS + + GST T+ S+ S T+ S Sbjct: 329 TAQKGSDLTAGYGSTGTAGDDSSLI----AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 384 Query: 1097 QSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSVS 1156 + S T+ +DS+ + ST ++ S + S + S T+ T T+ Sbjct: 385 TAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGD 444 Query: 1157 DSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQSASAFLSESLS 1216 DS+ ++ S + S+ + + ++ S + STS + +S+ S Sbjct: 445 DSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQ 504 Query: 1217 ESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKS 1276 + ST + ST + + SD + GSTST+ +NS+ + ST T+ S T Sbjct: 505 TAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGY 564 Query: 1277 ESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTS 1336 S T+ S T+ ST + S S + S ++ S+ + S + S Sbjct: 565 GSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVL 624 Query: 1337 LSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMN 1396 +G S S + + SS + ST + S T+G ST T+ SD T+ S S + Sbjct: 625 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGA 684 Query: 1397 QSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTS 1456 S + + S + S T+ S T+ S TS STST+ + S + ST Sbjct: 685 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQ 744 Query: 1457 QSGSTSTSASLSGSESESDSQSISTS--ASESTSESASTSLSDSTSTSNSGSASTSTSLS 1514 + S+ + GS + QS+ T+ S ST+ + S+ ++ ST +G S T+ Sbjct: 745 TASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGY 804 Query: 1515 NSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVS 1574 S ++ S T+ STS + S + S + S T+ ST + S Sbjct: 805 GSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDL 864 Query: 1575 TSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTS 1634 T+ GSTS + +S + S + S +G ST T+ +S T+ STS Sbjct: 865 TTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 924 Query: 1635 TSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVS 1680 S + ST T++ S + S + S+ + GS S++ Sbjct: 925 ESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMA 970 Score = 49.0 bits (116), Expect = 3e-07 Identities = 206/894 (23%), Positives = 361/894 (40%), Gaps = 6/894 (0%) Query: 733 TDASGNKTTTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTS 792 T G+ T + T + S ++ GSTQ + ST A S T+ GS + + Sbjct: 281 TAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGY 340 Query: 793 ASTSKSTSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDS 852 ST + S + S + +S+ + ST S ++ GS + + S Sbjct: 341 GSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSL 400 Query: 853 ISNSNSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLS 912 I+ ST+ + ST T+ T T+ S + GS + S+ I+G ST + Sbjct: 401 IAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGE 460 Query: 913 DSTSNAISTSTSLSESASTSDSISISNSIANSQSA------STSKSDSQSTSISLSTSDS 966 DS+ A ST ++ S + S S A +S+ ST + ST + S Sbjct: 461 DSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQ 520 Query: 967 KSMSTSESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDS 1026 + + S+ ++ STS + + S IA S T++ +S+ T+ S + GS + Sbjct: 521 TAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGY 580 Query: 1027 KSMSVSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSES 1086 S + S S+ +G S + S+ + S + QS T+ STS + S Sbjct: 581 GSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSL 640 Query: 1087 QSTSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS 1146 + GS + +S+ + S T+ S TA S S + + S+ + ST + Sbjct: 641 IAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGY 700 Query: 1147 NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQS 1206 NS T+ S T+ S+ S STST+ + S I+ ST + S+ T+ S Sbjct: 701 NSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQ 760 Query: 1207 ASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTS 1266 + S + S ST+ + SS + S + S T+ S T+ S T+ Sbjct: 761 TAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGY 820 Query: 1267 ISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTS 1326 S ST+ S ++ S T+ S T+ S + +S + S ST+ S+ Sbjct: 821 GSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSL 880 Query: 1327 KSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTS 1386 + ST T+ S + ST +++ SD T+ S S + S+ + S ++ Sbjct: 881 IAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASF 940 Query: 1387 TSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLS 1446 S ++ + S+ + STS + +S + T + QS T+ S Sbjct: 941 KSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQ 1000 Query: 1447 DSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGS 1506 + S T+ GST+T+ + S + S S S T+ ST +S S +G Sbjct: 1001 TAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGY 1060 Query: 1507 ASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTI 1566 S+ S S+ + S+ + S+ + S + + S ++ S+ T+ ST+ Sbjct: 1061 GSSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTL 1120 Query: 1567 ASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDS 1620 S + SV + + ++S T+ S + + S +G S T+ +D Sbjct: 1121 ISGADSVQMAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDC 1174 Score = 47.8 bits (113), Expect = 6e-07 Identities = 241/1091 (22%), Positives = 433/1091 (39%), Gaps = 10/1091 (0%) Query: 907 TSASLSDSTSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDS 966 TSA A + + ++ S ++ + N T D+ S S + + Sbjct: 99 TSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQT 158 Query: 967 KSMSTSESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDS 1026 ++T S T S ++G S + ST + ST +DS +G S + Sbjct: 159 IEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTA 218 Query: 1027 KSMSVSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSES 1086 S + S S + S + S + GST T+ S+ S Sbjct: 219 GEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 278 Query: 1087 QSTSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS 1146 T+ S + S T+ +DS+ + ST ++ S + S + S T+ Sbjct: 279 TQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTA 338 Query: 1147 NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQS 1206 T T+ DS+ ++ S + S+ + + ++ S + ST + + S Sbjct: 339 GYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADS 398 Query: 1207 ASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTS 1266 + S + EST + ST + SD T+ GST T+ +S+ + ST T+ Sbjct: 399 SLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTA 458 Query: 1267 ISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMS--TSDSIS 1324 +S+ T S T+ S T+ STS + S + S + S T+ S Sbjct: 459 GEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGS 518 Query: 1325 TSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTS------TS 1378 T + + S + GSTS + ++S+ + S T+ S+ + GST T+ T+ Sbjct: 519 TQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTA 578 Query: 1379 TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSES 1438 S T+ S S + S ++ S + ST T+ S T+ Y S ST+ ++S Sbjct: 579 GYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADS 638 Query: 1439 TSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTSLSDS 1498 + + S T+ S +G ST + GS+ + S ST+ ++S+ + S + Sbjct: 639 SLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTA 698 Query: 1499 TSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTS 1558 S + ST + S S STS + + S+ + S ++ S + S Sbjct: 699 GYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGS 758 Query: 1559 TSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTS 1618 T + STS +G+ S + ST T+ S T+ S + S T+ Sbjct: 759 TQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTT 818 Query: 1619 DSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMS 1678 STS + + S + S + S T+ ST + SD T+ S ST+G S Sbjct: 819 GYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDS 878 Query: 1679 VSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDS 1738 I+ ST T+ S + + S + S + S S + +S ++G S + Sbjct: 879 SLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTA 938 Query: 1739 GSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDS 1798 S + S + S + S S++ DSS ++ S +++ S + S Sbjct: 939 SFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGS 998 Query: 1799 KSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGS 1858 T+ +ST T+G ST+T+ + S ++ S S S T+ S +SG S+ + Sbjct: 999 TQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTA 1058 Query: 1859 TSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTS 1918 S+ S S + S + S+ ++ +S+ + ++ SM I+ S + Sbjct: 1059 GYGSSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNR--SMLIAGKGSSQTAGY 1116 Query: 1919 DSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMS 1978 S+ ISG++S + ++G+ S T+ S ++G+ S + S T+ +D + Sbjct: 1117 RSTLISGADSVQMAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDCIL 1176 Query: 1979 QSQSTSTSASG 1989 + S +G Sbjct: 1177 MAGDRSKLTAG 1187

SAI8T7_1000710

SAI8T7_1000830

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1000710	0	11	2.694207	Iron-regulated ABC transporter
SAI8T7_1000720	1	15	3.136375	Probable siderophore biosynthesis protein SbnA
SAI8T7_1000730	3	16	3.489799	Probable ornithine cyclodeaminase protein
SAI8T7_1000740	2	16	3.356143	Putative uncharacterized protein
SAI8T7_1000750	2	17	3.591111	Putative uncharacterized protein
SAI8T7_1000760	0	15	2.722747	Putative uncharacterized protein
SAI8T7_1000770	0	14	3.295728	Similar to siderophore biosynthesis protein
SAI8T7_1000780	-1	14	2.656087	Putative uncharacterized protein
SAI8T7_1000790	-2	13	1.873213	Probable diaminopimelate decarboxylase protein
SAI8T7_1000800	-2	11	1.277577	Putative uncharacterized protein sbnI
SAI8T7_1000810	-1	11	0.019912	Putative uncharacterized protein
SAI8T7_1000820	1	11	-0.255790	Diacetyl reductase [(S)-acetoin forming]
SAI8T7_1000830	2	12	-1.048501	Polysaccharide biosynthesis family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000710	FERRIBNDNGPP	69	2e-15	Ferrichrome-binding periplasmic protein signature.
>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature. Length = 296 Score = 68.8 bits (168), Expect = 2e-15 Identities = 46/191 (24%), Positives = 78/191 (40%), Gaps = 38/191 (19%) Query: 53 PKRVVTLYQGATDVAVSLGVKPVGAVES-----WTQKPKFEYIKNDLKDTKI-VGQEPAP 106 P R+V L ++ ++LG+ P G ++ W +P L D+ I VG P Sbjct: 35 PNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPP-------LPDSVIDVGLRTEP 87 Query: 107 NLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKD----------TTKLMG 156 NLE ++++KP +V S + L++IAP F F D + M Sbjct: 88 NLELLTEMKPSFMVWS-AGYGPSPEMLARIAPGR----GFNFSDGKQPLAMARKSLTEMA 142 Query: 157 KALGKEKEAEDLLKKYDDKVAAFQKDAKAKY--KDAWPLKASVVNF-RADHTRIYA-GGY 212 L + AE L +Y+D F + K ++ + A PL + H ++ Sbjct: 143 DLLNLQSAAETHLAQYED----FIRSMKPRFVKRGARPL--LLTTLIDPRHMLVFGPNSL 196 Query: 213 AGKILNDLGFK 223 +IL++ G Sbjct: 197 FQEILDEYGIP 207
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000730	SYCECHAPRONE	31	0.002	Gram-negative bacterial type III secretion SycE cha...
>SYCECHAPRONE#Gram-negative bacterial type III secretion SycE chaperone signature. Length = 130 Score = 31.2 bits (70), Expect = 0.002 Identities = 14/33 (42%), Positives = 16/33 (48%), Gaps = 1/33 (3%) Query: 21 VDALTEALTAHAHNDFVQ-PLKPYLRQDPENGH 52 +D E T +HN F Q LKP L D GH Sbjct: 54 LDNNDEKETLLSHNIFSQDILKPILSWDEVGGH 86
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000740	PF04183	316	e-103	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 316 bits (812), Expect = e-103 Identities = 119/527 (22%), Positives = 208/527 (39%), Gaps = 46/527 (8%) Query: 79 RVSKQPLTAAEFWQTIANMNCDLSHEWEVARVEEGLTTAATQLAKQLSELDLASHPFV-- 136 R + +P+ A + + +S +++ T L + L++ + Sbjct: 66 RCADEPVLAQTLLMQLKQVL-SMSDATVAEHMQDLYATLLGDLQLLKARRGLSASDLINL 124 Query: 137 -MSEQFASLKDRPFHPLAKEKRGLREADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANI 195 L P K +RG + + Y E +F L AVK+ HMI + Sbjct: 125 NADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEM 184 Query: 196 DELENLTVPIKEQA----TDMLNDQGLSIDDYVLFPVHPWQYQHILPNVFATEISEKLVV 251 D + LT + Q + + + GL +++ PVHPWQ+Q + F + +E +V Sbjct: 185 DIHQLLTAAMDPQEFARFSQVWQENGLD-HNWLPLPVHPWQWQQKIATDFIADFAEGRMV 243 Query: 252 LLPLKFGD-YLSSSSMRSLIDIGAPYN-HVKVPFAMQSLGALRLTPTRYMKNGEQAEQLL 309 L +FGD +L+ S+R+L + +K+P + + R P RY+ G A + L Sbjct: 244 SLG-EFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWL 302 Query: 310 RQLIEKDEALAKYVMV-CDETA-------WWSYMGQDNDIFKDQLGHLTVQLRKYPEVLA 361 +Q+ D L + V E A ++ + + +++ LG V R+ P Sbjct: 303 QQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLG---VIWRENPCRWL 359 Query: 362 KNDTQQLVSMAALAANDRTLYQMICGKDNISKNDVMTLFEDIAQVFLKVTLSFM-QYGAL 420 K D + V MA L D + + S D T + +V + + +YG Sbjct: 360 KPD-ESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVA 418 Query: 421 PELHGQNILLSFEDGRVQKCVLRD-HDTVRIYKPWLTAHQLSLPKYV--VREDTPNTLIN 477 HGQNI L+ ++G Q+ +L+D +R+ K SLP+ V V + Sbjct: 419 LIAHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMD-SLPQEVRDVTSRLSADYLI 477 Query: 478 EDLETFFAYFQTLAVSVNLYAIIDAIQDLFGVSEHELMSLLKQILKNEVATISWVTTDQL 537 DL+T V + I + GV E LL +L + + Q+ Sbjct: 478 HDLQTGHF--------VTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMK-----KHPQM 524 Query: 538 AVRHILFDKQTWPFKQILLP---LLY-QRDSGGGSMPSGLTTVPNPM 580 + R LF +++L L + D G +P+ L + NP+ Sbjct: 525 SERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEDLQNPL 571
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000750	TCRTETA	73	3e-16	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 72.9 bits (179), Expect = 3e-16 Identities = 66/333 (19%), Positives = 131/333 (39%), Gaps = 22/333 (6%) Query: 17 GIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTPLQFVLVRLL 76 GI +A A+ +P+ G L D+ R+ ++L +L G AV +MA + R++ Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105 Query: 77 QGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVTASILGFSALLM 136 G+ G + A+ + ++R + G + + G + GP++GG+ A Sbjct: 106 AGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGF-SPHAPFF 164 Query: 137 SIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQTCRFIIVGVLANF 196 + A + + + G L E+ + SF+ + V A Sbjct: 165 AAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFR--------WARGMTVVAALM 216 Query: 197 AMYGMLTALSPLASSVNHTAIDDR-----SVIGFLQSAF-WTASILSAPLWGRFNDKSYV 250 A++ ++ + + +++ +DR + IG +AF S+ A + G + Sbjct: 217 AVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE 276 Query: 251 KSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSAL--IQSVMFVVVNACHQ-QL 307 + + IA G IL AT +L + +Q+++ V+ Q QL Sbjct: 277 RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQL 336 Query: 308 KGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPA 340 +G+ T+ + I+G L AI + + Sbjct: 337 QGSLAALTS----LTSIVGPLLFTAIYAASITT 365
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000760	PF04183	300	2e-97	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 300 bits (769), Expect = 2e-97 Identities = 109/474 (22%), Positives = 192/474 (40%), Gaps = 52/474 (10%) Query: 11 WLIDGKSKKITTYKEAIARILQHMAQSADNQTA-VQQHMAQIMSDI--DNSIHRTARYLQ 67 ID ++ + +L + Q A V +HM + + + D + + R L Sbjct: 58 LWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLS 117 Query: 68 SNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSEADLEKYAPECHTSFQLHYLAVHQD 127 ++ + + Q L GHP K G+ + LE+YAPE +F+LH+LAV ++ Sbjct: 118 ASDL---INLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKRE 174 Query: 128 -------------VLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFILLPTHPYQINVLR 174 LLT ++ +E ++Q +D +++ LP HP+Q Sbjct: 175 HMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLD-----HNWLPLPVHPWQWQQK- 228 Query: 175 QHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVF--SKALNIYLKLPIHVKITNFIRTND 232 ++ +G + LG GD S+RT+ S+ + +KLP+ + T+ R Sbjct: 229 IATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIP 288 Query: 233 LEQIERTIDAAQVIASVKDE-----------VETPHFKLMFEEGYRALLPNPLGQTVEPE 281 I A++ + V + P + EGY AL P E Sbjct: 289 GRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQ---E 345 Query: 282 MDLLTNSAMIVREGIPNY-HADKDIHVLASLFETMPD-SPISKLAQVIEQSGLAPEAWLE 339 M +I RE + D+ ++A+L E + P+ I++SGL E WL Sbjct: 346 M-----LGVIWRENPCRWLKPDESPVLMATLMECDENNQPL--AGAYIDRSGLDAETWLT 398 Query: 340 CYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEG-ICLSRTIATEKQ 398 ++P+ L G++L AH QN + +K+G+P ++D +G + L + E Sbjct: 399 QLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMD 458 Query: 399 LVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLVA 452 +P V + + A D H L+ V L + + + E +QL+A Sbjct: 459 SLPQEVRDVTSRLSA-DYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQLLA 511
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000770	PF04183	497	e-173	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 497 bits (1282), Expect = e-173 Identities = 142/579 (24%), Positives = 251/579 (43%), Gaps = 40/579 (6%) Query: 1 MHQLVSSLIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVG 60 + +++S L YE + + A Q G + I +++RF AE+ + + I + R Sbjct: 14 VAKMLSELEYEQV--FHAESQ-GDDRYCINLPGAQWRFIAERG-IWGWLWIDAQTLRC-- 67 Query: 61 DEADTTTDYTQLLREAVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFN 120 AD LL + +D + + + +L T L D Q ++ R + N Sbjct: 68 --ADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASDLINLN 125 Query: 121 -DYEFYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVV 179 D + GH K R G+ ++ P++ +L WLA+ ++ + + Sbjct: 126 ADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEMD 185 Query: 180 VNEMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW 239 ++++L + + + F Q + +G N + +PVHPWQ++ I D + G ++ Sbjct: 186 IHQLLTAAMDPQEFARFSQVWQENGLDHNWL-PLPVHPWQWQQKIATDFIADFAEGRMVS 244 Query: 240 LGESDELYHPQQSIRTMSPIDTT-KYYLKVPISITNTSTKRVLAPHTIENAAQITDWLKQ 298 LGE + + QQS+RT++ +K+P++I NTS R + I + WL+Q Sbjct: 245 LGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWLQQ 304 Query: 299 IQQQDMYLKDE----LKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLID 354 + D L L G V + Y +PY+ ++ LGVIWREN L Sbjct: 305 VFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEM---LGVIWRENPCRWLKP 361 Query: 355 EEDAIPFNALYASDKDGLPFIEKWIKQYG--SEAWTKQFLAVAIRPMIHMLYYHGIAFES 412 +E + L D++ P +I + G +E W Q V + P+ H+L +G+A + Sbjct: 362 DESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALIA 421 Query: 413 HAQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIET 472 H QN+ L + G P R+ LKDF +R +E E S +P+ + V S Sbjct: 422 HGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMDS------LPQEVRDVTS------ 469 Query: 473 DDERLVRDFLH---DAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN 529 RL D+L F+ + I + + G+ E+R +Q + ++ Y + P+++ Sbjct: 470 ---RLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMKKHPQMSE 526 Query: 530 -YQHFDLFEPTIQVEKLTTRRL-LSDSELRIHHVTNPLG 566 + F LF P I L +L D + + N L Sbjct: 527 RFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLE 565
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000820	DHBDHDRGNASE	128	4e-38	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 128 bits (323), Expect = 4e-38 Identities = 66/250 (26%), Positives = 113/250 (45%), Gaps = 2/250 (0%) Query: 5 KVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAAALKLSSDGTKAIAIKADVSN 64 K+A +TG AQGIG +A L G +A VD+N E + L ++ A A ADV + Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 65 RDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITEEQFKTVYGVNVAGVLWGIQAAH 124 + + + G ++VN AG+ I ++++E+++ + VN GV ++ Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 125 EQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFAVRGLTQVAAQDLASEGITVNAFAP 184 + G I+ S ++ Y S+K A T+ +LA I N +P Sbjct: 129 KYMMD-RRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 185 GIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQIALGRVSQPEDVSNVVSFLAGKDSDYI 244 G +T M S+ + E F + I L ++++P D+++ V FL + +I Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSL-ETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246 Query: 245 TGQTIIVDGG 254 T + VDGG Sbjct: 247 TMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1000830	NUCEPIMERASE	217	9e-71	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 217 bits (554), Expect = 9e-71 Identities = 79/327 (24%), Positives = 139/327 (42%), Gaps = 33/327 (10%) Query: 6 RVLITGGAGFIGSHLVDDL-QQDYDVYVLDNYRTG-----KRENIKSLADDHVF--ELDI 57 + L+TG AGFIG H+ L + + V +DN K+ ++ LA ++D+ Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61 Query: 58 REYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVATLRLLEIIKKYNSHIK 117 + + + + + F+ V ++V S+E P + N+ L +LE + I+ Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK--IQ 119 Query: 118 RFIFASSAAVYGDLPDLPKSDQSLI-LPLSPYAIDKYYGERTTLNYCSLYNIPTAVVKFF 176 ++ASS++VYG +P S + P+S YA K E Y LY +P ++FF Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFF 179 Query: 177 NVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDFVYVYDVVQSVRLIMEH-- 234 V+GP P M K + G RDF Y+ D+ +++ + + Sbjct: 180 TVYGPWGRPDMALFKFTKAML----EGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIP 235 Query: 235 ---------------KDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARKGDI 279 A YNIG + L++ + + + G + + GD+ Sbjct: 236 HADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGDV 295 Query: 280 KHSYADISNL-KALGFVPKYTVETGLK 305 + AD L + +GF P+ TV+ G+K Sbjct: 296 LETSADTKALYEVIGFTPETTVKDGVK 322

SAI8T7_1001280

SAI8T7_1001320

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1001280	3	14	1.468170	Similar to integral membrane protein LmrP
SAI8T7_1001290	3	13	1.731674	Gramicidin S synthetase 2 related protein
SAI8T7_1001300	2	14	2.429596	Putative uncharacterized protein
SAI8T7_1001310	2	15	2.006495	Putative uncharacterized protein
SAI8T7_1001320	1	15	1.927579	Acetylglutamate kinase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001280	TCRTETA	32	0.004	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 32.1 bits (73), Expect = 0.004 Identities = 61/337 (18%), Positives = 127/337 (37%), Gaps = 33/337 (9%) Query: 7 TLKVRLISNFLQLIITTAFIPFIALYLTDMLS----QSIVGIYLVGLVVLKFPLSIISGY 62 L V L + L + +P + L D++ + GI L +++F + + G Sbjct: 6 PLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGA 65 Query: 63 LIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQI-IGFCVAYAIFTIVWGLQFPVMDT 121 L + F ++ ++L+ A + M + LW + IG VA + G V Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMAT--APFLWVLYIGRIVAG-----ITGATGAVAGA 118 Query: 122 LIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGYSMLLLFLIAACIFLIVLFILY 181 I D D + + G +LGGLM G+S F AA + + Sbjct: 119 YIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGC 178 Query: 182 IWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLVLKDRNYMLLISGFSIIMMGEFSI 241 LP+ ++ + + + L+ F + ++G+ Sbjct: 179 FLLPESHKGERRPLRREALNPLASFRWARGMTVVAA--------LMAVFFIMQLVGQVPA 230 Query: 242 SSYIAIRLKDQF--ETISIGSYDITGAKMLAILLMINTVVVILLTYSISKVVLKIDFKKA 299 + + I +D+F + +IG LA +++++ ++T ++ ++ ++A Sbjct: 231 ALW-VIFGEDRFHWDATTIGI-------SLAAFGILHSLAQAMITGPVAA---RLGERRA 279 Query: 300 LITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGEII 336 L+ G++ GY L + + + M++ G I Sbjct: 280 LMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIG 316
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001290	NUCEPIMERASE	53	8e-09	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 52.9 bits (127), Expect = 8e-09 Identities = 54/266 (20%), Positives = 101/266 (37%), Gaps = 55/266 (20%) Query: 2052 NTLLTGATGFLGAYLIEALQGYSHRIYCFIRADNEEIAWYKLMTNLNDYFS----EETVE 2107 L+TGA GF+G ++ + L H++ + D NLNDY+ + +E Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQV---VGID-----------NLNDYYDVSLKQARLE 47 Query: 2108 MM----LSNIEVIVGDFECMDDVVLPENMDTIIH----AGARTDHFGDDDEFEKVNVQGT 2159 ++ ++ + D E M D+ + + + R + + N+ G Sbjct: 48 LLAQPGFQFHKIDLADREGMTDLFASGHFERVFISPHRLAVR-YSLENPHAYADSNLTGF 106 Query: 2160 VDVIRLAQQHH-ARLIYVSTISV-GTYFDIDTEDVTFSEADVYKGQLLTSPYTRSKFYSE 2217 ++++ + + L+Y S+ SV G + FS D + S Y +K +E Sbjct: 107 LNILEGCRHNKIQHLLYASSSSVYG-----LNRKMPFSTDDSVDHPV--SLYAATKKANE 159 Query: 2218 LKVLEAVNN-GLDGRIVRVGNLTSPYNGRWHM------RNIKTNRFSMVMNDLLQLDCIG 2270 L + GL +R + P+ GR M + + + V N Sbjct: 160 LMAHTYSHLYGLPATGLRFFTVYGPW-GRPDMALFKFTKAMLEGKSIDVYNY-------- 210 Query: 2271 VSMAEMPVDFSFVDTTARQIVALAQV 2296 +M DF+++D A I+ L V Sbjct: 211 ---GKMKRDFTYIDDIAEAIIRLQDV 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001300	ENTSNTHTASED	29	0.009	Enterobactin synthetase component D signature.
>ENTSNTHTASED#Enterobactin synthetase component D signature. Length = 234 Score = 29.2 bits (65), Expect = 0.009 Identities = 15/57 (26%), Positives = 27/57 (47%), Gaps = 5/57 (8%) Query: 84 GQP-----IYVSLSYSYPYIVCVVDKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQI 135 QP ++ S+S+ + V+ ++ +GIDIEKI + L ++ QI Sbjct: 76 RQPLWPDGLFGSISHCATTALAVISRQRIGIDIEKIMSQHTATELAPSIIDSDERQI 132
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001320	CARBMTKINASE	32	0.002	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 31.7 bits (72), Expect = 0.002 Identities = 23/84 (27%), Positives = 41/84 (48%), Gaps = 7/84 (8%) Query: 155 INADTLAYFIASSLKAPIYV-LSNIAGVLIN-----DVVIPQLPLVDIHQYIEHGD-IYG 207 I+ D +A + A I++ L+++ G + + + ++ + ++ +Y E G G Sbjct: 213 IDKDLAGEKLAEEVNADIFMILTDVNGAALYYGTEKEQWLREVKVEELRKYYEEGHFKAG 272 Query: 208 GMIPKVLDAKNAIENGCPKVIIAS 231 M PKVL A IE G + IIA Sbjct: 273 SMGPKVLAAIRFIEWGGERAIIAH 296

SAI8T7_1001680

SAI8T7_1001750

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1001680	1	18	1.484855	MFS family major facilitator transporter, hexose
SAI8T7_1001690	1	14	0.302156	Uncharacterized response regulatory protein
SAI8T7_1001700	0	15	0.601654	Uncharacterized sensor-like histidine kinase
SAI8T7_1001710	-2	14	2.074289	Putative Similar to periplasmic-iron-binding
SAI8T7_1001720	-1	14	2.615695	Formate acetyltransferase
SAI8T7_1001730	-2	13	2.798598	Pyruvate formate-lyase-activating enzyme
SAI8T7_1001740	-1	13	2.931975	Glycerophosphoryl diester phosphodiesterase
SAI8T7_1001750	-1	14	4.075711	Staphylocoagulase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001680	TCRTETA	38	8e-05	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 37.9 bits (88), Expect = 8e-05 Identities = 53/361 (14%), Positives = 121/361 (33%), Gaps = 40/361 (11%) Query: 36 AFFVVFFVYMAMYLIRNNFKAAQPFLKEEIGLSTLELGYIGL---AFSITYGLGKTLLGY 92 V + + LI P L ++ S + G+ +++ +LG Sbjct: 10 ILSTVALDAVGIGLI----MPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGA 65 Query: 93 FVDGRNTKRIISFLLILSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYST 152 D + ++ L +A+ IM + F V+ + ++ G+ G G + + Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMAT--APFLWVLYIGRIVAGITG----ATGAVAGAY 119 Query: 153 ISRWAPRTKRGRYLGFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGI 212 I+ +R R+ GF + G + H F + + L + Sbjct: 120 IADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAP----FFAAAALNGLNFL 175 Query: 213 ATLFIGKDDPEELGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNV 272 F+ + + + P+ +E ++ +W V ++ V + Sbjct: 176 TGCFLLPE---------SHKGERRPLRREALNPLASFRWARGMT-----VVAALMAVFFI 221 Query: 273 FVYIVRIGIDNWAPLYVSEHLHFSKGDAVNTIFYFEI-GALVASLLWGYVSDLLKGRRAI 331 + ++ W ++ + H+ ++ F I +L +++ G V+ L RRA+ Sbjct: 222 MQLVGQVPAALWV-IFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRAL 280 Query: 332 VAIGCMFMITFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVAN 391 + +++L + + + L A G I P +L + + Sbjct: 281 MLGMIADGTGYILLAFATRGWMAFPIMVLLASGG-IGMP------ALQAMLSRQVDEERQ 333 Query: 392 G 392 G Sbjct: 334 G 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001690	HTHFIS	44	1e-07	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 44.1 bits (104), Expect = 1e-07 Identities = 30/114 (26%), Positives = 50/114 (43%), Gaps = 9/114 (7%) Query: 1 MPRKNGVDLLNDI--ALLDCNVIILSSYDDFEYMKAGIQHHVLDYLLKPVDHAQLEVILG 58 MP +N DLL I A D V+++S+ + F + DYL KP D L ++G Sbjct: 57 MPDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFD---LTELIG 113 Query: 59 RLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIKQSYQTKVTV 112 + R L E + + + D PL+ + +I + + QT +T+ Sbjct: 114 IIGRALAEPKRRPSKLEDDSQD-GMPLVG---RSAAMQEIYRVLARLMQTDLTL 163
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001700	PF06580	146	6e-42	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 146 bits (371), Expect = 6e-42 Identities = 55/226 (24%), Positives = 109/226 (48%), Gaps = 16/226 (7%) Query: 278 YIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTIQYLITLSPKLAQT 337 + + F++ +Q ++ QL ++ Q PHF+FN + I+ LI P A+ Sbjct: 136 FGWHFFKNYKQAEIDQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKARE 195 Query: 338 VVQQLSQMLRYSLR-TNSHTVELNEELNYIEQYVAIQNIRFDDMIKLHIESSEEARHQTI 396 ++ LS+++RYSLR +N+ V L +EL ++ Y+ + +I+F+D ++ + + + Sbjct: 196 MLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQV 255 Query: 397 GKMMLQPLIENAIKHGRDTESLDITIRLTLARQN--LHVLVCDNGIGMSSSRLQYVRQSL 454 M++Q L+EN IKHG I L + N + + V + G L+ ++S Sbjct: 256 PPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLA----LKNTKES- 310 Query: 455 NNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYKIP 500 GL ++ + + YG+ A++ + K+ + + IP Sbjct: 311 -------TGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVL-IP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001720	SHAPEPROTEIN	32	0.006	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 32.4 bits (74), Expect = 0.006 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 5/54 (9%) Query: 262 AYLAAIKEQNGAAMSLGRTSTFLDIYAERDLKAGVITESEV-QEIIDHFIMKLR 314 +AA+ A LGRT +I A R +K GVI + V ++++ HFI ++ Sbjct: 50 KSVAAVGHD--AKQMLGRTPG--NIAAIRPMKDGVIADFFVTEKMLQHFIKQVH 99
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1001750	IGASERPTASE	32	0.009	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.0 bits (72), Expect = 0.009 Identities = 37/215 (17%), Positives = 66/215 (30%), Gaps = 16/215 (7%) Query: 249 ETKQNRPNSITKYDPTKHNFKEKSENKPNFDKLVEETKKAVKEADESWKNKTVKKYEETV 308 E+K N + T N + E K N + + A ++ T K TV Sbjct: 1047 ESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATV 1106 Query: 309 TKSPVVKEEKKVEEPQLPKVGNQQEVKTTAGKAEETTQPVAQPLVKIPQETIYGETVKGP 368 K +E+ KVE + +V + + ET QP A+P TV Sbjct: 1107 EK----EEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEP------ARENDPTVNIK 1156 Query: 369 EYPTMENKTLQGEIVQGPDFLTMEQNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQG 428 E + N + Q P T ++++ T T + + + + +P Sbjct: 1157 EPQSQTNT--TADTEQ-PAKETSSNVEQPVTESTTVNTGNSVVENPENTTPA--TTQPTV 1211 Query: 429 TESTLKGIQGESSDIEVKPQATETTEASQYGPRPQ 463 + + V+ A+ Sbjct: 1212 NSESSNKPK-NRHRRSVRSVPHNVEPATTSSNDRS 1245

SAI8T7_1003010

SAI8T7_1003140

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1003010	-2	14	-0.479160	Putative nucleoside-diphosphate-sugar epimerase
SAI8T7_1003020	2	16	-1.075687	Exotoxin=6
SAI8T7_1003030	1	15	-1.175554	Exotoxin=7
SAI8T7_1003040	3	14	-1.689685	Putative uncharacterized protein (fragment)
SAI8T7_1003050	4	15	-2.798544	Exotoxin=8
SAI8T7_1003060	2	16	-2.991972	Superantigen-like protein
SAI8T7_1003070	2	15	-1.204044	Superantigen-like protein 5
SAI8T7_1003080	1	13	-1.942571	Exotoxin=11
SAI8T7_1003090	1	13	-2.000277	Superantigen-like protein 7
SAI8T7_1003100	3	9	-1.605994	Exotoxin=13
SAI8T7_1003110	4	9	-1.710183	Exotoxin=14
SAI8T7_1003120	4	11	-1.932228	Type I restriction enzyme EcoR124II M protein
SAI8T7_1003130	10	14	-3.878715	Type I restriction modification DNA specificity
SAI8T7_1003140	10	13	-3.605067	Exotoxin=15

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003010	NUCEPIMERASE	30	0.009	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 30.1 bits (68), Expect = 0.009 Identities = 28/167 (16%), Positives = 61/167 (36%), Gaps = 32/167 (19%) Query: 1 MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPD----------DWRGKVSVRQ 50 M ++TGA G +G H++ + + H +G+ N+ D + + Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEA--GHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHK 58 Query: 51 LDYFNQESMVEAFK--GMDTVVFI-------PSIIHP-SFKRIPEV--ENLVYAAKQSGV 98 +D ++E M + F + V S+ +P ++ N++ + + + Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118 Query: 99 AHIIFIG---YYADQHNNPFHMS-----PYFGYASRLLSTSGIDYTY 137 H+++ Y PF P YA+ + + +TY Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTY 165
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003020	TOXICSSTOXIN	95	3e-26	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 95.5 bits (237), Expect = 3e-26 Identities = 46/214 (21%), Positives = 84/214 (39%), Gaps = 11/214 (5%) Query: 18 TGVITSNVQSVQAKTEVKQQSESELKHYYNKPVLERKNVTGYKYTEKGKDYIDVIVDNQY 77 T V S+ Q ++ + +L +Y+ N + + + + + Sbjct: 25 TPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNS---EVLDNSLGSMRIKNTDGS 81 Query: 78 SQISLVGSDKDKFKDGDNSNIDVFILREGDSRQATN-----YSIGGVTKTNSQPFIDYIH 132 + + S +D+ R S+ + + I GVT T P I Sbjct: 82 ISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLP--TPIE 139 Query: 133 TPILEIKKGKEEPQSSLYQIYKEDISLKELDYRLRERAIKQHGLYSNGLKQGQI-TITMK 191 P+ GK+ P + K+ +++ LD+ +R + + HGLY + K G ITM Sbjct: 140 LPLKVKVHGKDSPLKYGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMN 199 Query: 192 DGKSHTIDLSQKLEKERMGDSIDGRQIQKILVEM 225 DG ++ DLS+K E I+ +I+ I E+ Sbjct: 200 DGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003030	TOXICSSTOXIN	80	3e-21	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 80.5 bits (198), Expect = 3e-21 Identities = 32/168 (19%), Positives = 63/168 (37%), Gaps = 18/168 (10%) Query: 1 MNIIDGNSVNNLALIGKDKQHYHTGVHRNLNIFYVN-----EDKRFEGAKYSIGGITSAN 55 M I + + +L + +++ + I G+T+ Sbjct: 73 MRIKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTE 132 Query: 56 DKA--VDLIAEARVIKADHIGEYDYDFFPFKIDKEAMSLKEIDFKLRKYLIDNYGLYGEM 113 ++L + +V D +Y F DK+ +++ +DF++R L +GLY Sbjct: 133 KLPTPIELPLKVKVHGKDSPLKYGPKF-----DKKQLAISTLDFEIRHQLTQIHGLYRSS 187 Query: 114 ST----GKITVKKKYYGKYTFELDKKLQEDRMSDVINVTDIDRIEIKV 157 KIT+ Y +L KK + + IN+ +I IE ++ Sbjct: 188 DKTGGYWKITMNDG--STYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003050	TOXICSSTOXIN	89	5e-24	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 88.9 bits (220), Expect = 5e-24 Identities = 28/128 (21%), Positives = 48/128 (37%), Gaps = 5/128 (3%) Query: 67 VFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSVTKKDNQGMISRDVSEYMITKEEIS 126 ++ + + G+T T + L V + K++++ Sbjct: 109 TKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYG---PKFDKKQLA 165 Query: 127 LKELDFKLRKQLIEKHNLYGNM--GSGTIVIKMKNGGKYTFELHKKLQEHRMADVIEGTN 184 + LDF++R QL + H LY + G I M +G Y +L KK + + I Sbjct: 166 ISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDE 225 Query: 185 IDKIEVNI 192 I IE I Sbjct: 226 IKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003060	TOXICSSTOXIN	101	8e-28	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 101 bits (253), Expect = 8e-28 Identities = 45/216 (20%), Positives = 81/216 (37%), Gaps = 13/216 (6%) Query: 87 TKVETPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNIVPDYF 146 T V + K N KDL +Y+ S F N ++ ++R N Sbjct: 25 TPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTN-SEVLDNSLGSMRIKNTDGSI- 82 Query: 147 IYKIALVGKDDKKYDEGVHRNVDVFVVLEEKNKYGVE----RYSVGGITKSNSKKVDHKA 202 + + VD+ +K+++ E + + G+T + + Sbjct: 83 --SLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIEL 140 Query: 203 GVRITKEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIENHNLYGNV--GSGKIVIN 260 +++ + + K K+Q+++ LDF++R QL + H LY + G I Sbjct: 141 PLKVKVHGKDSPLKYG---PKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKIT 197 Query: 261 MKNGGKYTFELHKKLQENRMADVIDGTNIDNIEVNI 296 M +G Y +L KK + N I+ I IE I Sbjct: 198 MNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003070	TOXICSSTOXIN	135	2e-41	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 135 bits (340), Expect = 2e-41 Identities = 49/201 (24%), Positives = 73/201 (36%), Gaps = 14/201 (6%) Query: 44 NVTKDIFDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKAR 103 + +I DL D+YS S N S G + + IF Sbjct: 41 STNDNIKDLLDWYSSGSDTFTNSEVLDNSLGS---MRIKNTDGSISLIIFPSPYYSPAFT 97 Query: 104 KNPGLDI-----FVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIAT 158 K +D+ + F GVT + I P +K D Sbjct: 98 KGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLP--TPIELPLK-VKVHGKDSPLK 154 Query: 159 YGRVHYIYKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKI-KVIMKDGGYYTFELNKKL 217 YG K+++++ LDF++R L Q LY+ K K+ M DG Y +L+KK Sbjct: 155 YG--PKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKF 212 Query: 218 QTNRMSDVIDGRNIEKIEANI 238 + N I+ I+ IEA I Sbjct: 213 EYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003080	TOXICSSTOXIN	192	1e-63	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 192 bits (488), Expect = 1e-63 Identities = 49/197 (24%), Positives = 82/197 (41%), Gaps = 16/197 (8%) Query: 42 DIKDLYRYYSSESFEFSNI--------SGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDK 93 +IKDL +YSS S F+N S +++N +GS + F G+ Sbjct: 45 NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGE---- 100 Query: 94 YKKGLEGQNVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSF 153 K L + + + + GVT + L V KV+G + Sbjct: 101 -KVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKV-KVHGKDSPLKYGP- 157 Query: 154 LINKEEVSLKELDFKIRKQLVEKYGLYKGTTKYGKI-TINLKDEKKEVIDLGDKLQFERM 212 +K+++++ LDF+IR QL + +GLY+ + K G I + D DL K ++ Sbjct: 158 KFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTE 217 Query: 213 GDVLNSKDIQNIAVTIN 229 +N +I+ I IN Sbjct: 218 KPPINIDEIKTIEAEIN 234
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003090	TOXICSSTOXIN	125	2e-37	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 125 bits (314), Expect = 2e-37 Identities = 47/199 (23%), Positives = 73/199 (36%), Gaps = 19/199 (9%) Query: 44 DTNKLHQYYSGPSYELTNV--------SGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENK 95 + L +YS S TN S + + S L+ F G+ Sbjct: 45 NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGE---- 100 Query: 96 YKEKTHGLDVFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSID 155 K + + F +SGVT L L K+ KD + Sbjct: 101 -KVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELP----LKVKVHGKDSP-LK 154 Query: 156 EFFFIQKEEVSLKELDFKIRKLLIKKYKLYEGSA-DKGRIVINMKDENKYEIDLSDKLDF 214 K+++++ LDF+IR L + + LY S G I M D + Y+ DLS K ++ Sbjct: 155 YGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEY 214 Query: 215 ERMADVINSEQIKNIEVNL 233 IN ++IK IE + Sbjct: 215 NTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003100	TOXICSSTOXIN	130	1e-39	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 130 bits (329), Expect = 1e-39 Identities = 39/197 (19%), Positives = 69/197 (35%), Gaps = 15/197 (7%) Query: 43 INMLHQYYSEESFESTNISVKSEDYYGSNVLNFNQRNKTFKVFLLGDDKNKY------KE 96 I L +YS S TN V + + + + + K Sbjct: 46 IKDLLDWYSSGSDTFTNSEVLD---NSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKV 102 Query: 97 KTHGLDVFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKHGFSINELF 156 + + + + G+T + P L+VK F Sbjct: 103 DLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLP--TPIELP-LKVKVHGKDSPLKYGPKF 159 Query: 157 FIQKEEVSLKELDFKIRKMLVEKYRLYKGAS-DKGRIVINMKDEKKYVIDLSEKLSFDRM 215 K+++++ LDF+IR L + + LY+ + G I M D Y DLS+K ++ Sbjct: 160 --DKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTE 217 Query: 216 FDVMDSKQIKNIEVNLN 232 ++ +IK IE +N Sbjct: 218 KPPINIDEIKTIEAEIN 234
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003110	TOXICSSTOXIN	193	4e-64	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 193 bits (491), Expect = 4e-64 Identities = 51/202 (25%), Positives = 92/202 (45%), Gaps = 10/202 (4%) Query: 31 KQNQKSVNKHDKEALYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKF 90 K + S N + K+ L Y +G + N L + ++R K I +++ + Sbjct: 36 KTAKASTNDNIKDLLDWYSSG-SDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSP 94 Query: 91 QQRSYEGLDVFFVQEKRDKHD-----IFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAF 145 E +D+ + K+ +H I + + GV K + P L + K G+D+ Sbjct: 95 AFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELP-LKV-KVHGKDSP 152 Query: 146 VKGYPYYIKKEKITLKELDYKLRKHLIEKYGLYKTISKDGRV-KISLKDGSFYNLDLRSK 204 +K Y K+++ + LD+++R L + +GLY++ K G KI++ DGS Y DL K Sbjct: 153 LK-YGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKK 211 Query: 205 LKFKYMGEVIESKQIKDIEVNL 226 ++ I +IK IE + Sbjct: 212 FEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1003140	TOXICSSTOXIN	108	4e-31	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 108 bits (270), Expect = 4e-31 Identities = 47/225 (20%), Positives = 86/225 (38%), Gaps = 19/225 (8%) Query: 16 LTTGMITTTAQPVKASTLEVRSQAT-------QDLSEYYKGRGFELTNVTGYKYG-NKVT 67 L T PV S+ ++ A +DL ++Y TN + Sbjct: 15 LLLATTATDFTPVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTFTNSEVLDNSLGSMR 74 Query: 68 FIDNSQQIDVTLTGNE----KLTVKDDDEVSNVDVFVVREGSDKSAITTSIGGITKTNGT 123 + I + + + T + +++ + S+ + I I G+T T Sbjct: 75 IKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQISGVTNTE-- 132 Query: 124 QHKDTVQNVNLSVSKSTGQHTTSVTSEYYSIYKEEISLKELDFKLRKHLIDKHDLYKTEP 183 T + L V K G+ S K+++++ LDF++R L H LY++ Sbjct: 133 -KLPTPIELPLKV-KVHGK--DSPLKYGPKFDKKQLAISTLDFEIRHQLTQIHGLYRSSD 188 Query: 184 KDSKI-RITMKNGGYYTFELNKKLQPHRMGDTIDSRNIEKIEVNL 227 K +ITM +G Y +L+KK + + I+ I+ IE + Sbjct: 189 KTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233

SAI8T7_1006170

SAI8T7_1006240

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1006170	5	15	1.254877	Putative uncharacterized protein
SAI8T7_1006180	4	14	1.209504	Transposase for insertion sequence element IS256
SAI8T7_1006190	4	15	0.626807	Clumping factor A
SAI8T7_1006200	0	12	-2.151795	Coagulase family protein
SAI8T7_1006210	1	13	-1.650844	Putative Extracellular matrix protein-binding
SAI8T7_1006220	-1	15	-1.933508	Putative uncharacterized protein
SAI8T7_1006230	-1	12	-1.550712	Thermonuclease
SAI8T7_1006240	0	12	-1.827080	Putative uncharacterized protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006170	ALARACEMASE	27	0.049	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 26.7 bits (59), Expect = 0.049 Identities = 13/37 (35%), Positives = 19/37 (51%), Gaps = 2/37 (5%) Query: 135 MYDIYP-PYDGIPDEAFLI-KELKVNSLAGKTGTINY 169 D+ P P GI L KE+K++ +A GT+ Y Sbjct: 305 AVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGY 341
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006190	ICENUCLEATIN	43	7e-06	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 42.8 bits (100), Expect = 7e-06 Identities = 72/369 (19%), Positives = 131/369 (35%), Gaps = 6/369 (1%) Query: 514 SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD 573 S G+DS + S +G +ST +G S + SD + S + DS+ Sbjct: 294 STGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLI 353 Query: 574 SDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSD 633 + S + DS + S + SD + S + +DS+ + S + + Sbjct: 354 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 413 Query: 634 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 693 S + S + SD + S + DS + S + DS + S Sbjct: 414 STQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQT 473 Query: 694 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 753 + SD + S S + +S + S + S + S + ++SD + Sbjct: 474 AQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYG 533 Query: 754 SDSDSDSDSD------SDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSD 807 S S + ++S S + +S + S + SD + S + SDS Sbjct: 534 STSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSII 593 Query: 808 SESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD 867 + S + S + S + S +G S S++ +DS + GS + + Sbjct: 594 AGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYN 653 Query: 868 SNSDSESGS 876 S + GS Sbjct: 654 SILTAGYGS 662 Score = 42.8 bits (100), Expect = 7e-06 Identities = 74/379 (19%), Positives = 135/379 (35%), Gaps = 6/379 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + E+S G S + S +G ST +G DS+ + S + DS Sbjct: 309 GSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSL 368 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + SD + S + +DS+ + S + +S + S + Sbjct: 369 TAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQK 428 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 SD + S + DS + S + DS + S + SD + S S Sbjct: 429 GSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTS 488 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD----- 739 + +S + S + S + S + ++SD + S S + ++S Sbjct: 489 TAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGY 548 Query: 740 -SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDS 798 S + +S + S + SD + S + SDS + S + S Sbjct: 549 GSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSL 608 Query: 799 DSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDT 858 + S + S + S S + +DS + S +G +S ++ S T+ Sbjct: 609 TAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQE 668 Query: 859 GSDNDSDSDSNSDSESGSN 877 GSD + S S + + S+ Sbjct: 669 GSDLTAGYGSTSTAGADSS 687 Score = 42.0 bits (98), Expect = 1e-05 Identities = 69/363 (19%), Positives = 125/363 (34%), Gaps = 6/363 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + EDS G S + S +G ST +G+DS+ + S + +S Sbjct: 261 GSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQ 320 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + SD + S + DS+ + S + DS + S + Sbjct: 321 TAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQK 380 Query: 625 DSDSDSDSDSDSDSDSDSD------SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 678 SD + S + +DS S + +S + S + SD + S Sbjct: 381 GSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTG 440 Query: 679 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 738 + DS + S + DS + S + SD + S S + +S + Sbjct: 441 TAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGY 500 Query: 739 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDS 798 S + S + S + ++SD + S S + ++S + S + +S Sbjct: 501 GSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVL 560 Query: 799 DSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDT 858 + S + SD + S + SDS + S + S ++ S T+ Sbjct: 561 TAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTARE 620 Query: 859 GSD 861 S Sbjct: 621 QSV 623 Score = 42.0 bits (98), Expect = 1e-05 Identities = 72/369 (19%), Positives = 132/369 (35%), Gaps = 6/369 (1%) Query: 514 SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASD 573 S G DS + S +G DS+ +G S + SD + S + +DS+ Sbjct: 342 STGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLI 401 Query: 574 SDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSD 633 + S + +S + S + SD + S + DS+ + S + D Sbjct: 402 AGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGED 461 Query: 634 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 693 S + S + SD + S S + +S + S + S + S Sbjct: 462 SSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQT 521 Query: 694 SDSDSDSDSDSDSDSDSDSDSD------SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 747 + ++SD + S S + ++S S + +S + S + SD + Sbjct: 522 AQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYG 581 Query: 748 SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSD 807 S + SDS + S + S + S + S + S S + +DS Sbjct: 582 STGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLI 641 Query: 808 SESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD 867 + S + +S + S + SD +G S S++ +DS + GS + + Sbjct: 642 AGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYN 701 Query: 868 SNSDSESGS 876 S + GS Sbjct: 702 SILTAGYGS 710 Score = 41.3 bits (96), Expect = 2e-05 Identities = 76/379 (20%), Positives = 138/379 (36%), Gaps = 6/379 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSA------SDSDSASDSDS 558 G + EDS G S + S +G ST +G+DS+ S + +S Sbjct: 357 GSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQ 416 Query: 559 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 618 + S + SD + S + DS+ + S + DS + S + Sbjct: 417 TAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQK 476 Query: 619 ASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 678 SD + S S + +S + S + S + S + ++SD + S S Sbjct: 477 GSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTS 536 Query: 679 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 738 + ++S + S + +S + S + SD + S + SDS + Sbjct: 537 TAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGY 596 Query: 739 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDS 798 S + S + S + S + S S + +DS + S + +S Sbjct: 597 GSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSIL 656 Query: 799 DSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDT 858 + S ++ SD + S S + +DS + S +G +S ++ S T+ Sbjct: 657 TAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQE 716 Query: 859 GSDNDSDSDSNSDSESGSN 877 GSD S S S + + S+ Sbjct: 717 GSDLTSGYGSTSTAGADSS 735 Score = 41.3 bits (96), Expect = 2e-05 Identities = 74/372 (19%), Positives = 133/372 (35%), Gaps = 2/372 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + + SD G S + DS +G ST +G DS+ + S + SD Sbjct: 229 GSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 288 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + + S + S + +S + S + SD + S + Sbjct: 289 TAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGD 348 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + DS + S + SD + S + +DS + S Sbjct: 349 DSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQ 408 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + +S + S + SD + S + DS + S + DS + Sbjct: 409 TAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGY 468 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD + S S + +S + S + S + S + ++SD Sbjct: 469 GSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDL 528 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 + S S + ++S + S + +S +G S ++ SD T+ GS + Sbjct: 529 ITGYGSTSTAGANSSLIAGYGSTQTASYNSV--LTAGYGSTQTAREGSDLTAGYGSTGTA 586 Query: 865 DSDSNSDSESGS 876 SDS+ + GS Sbjct: 587 GSDSSIIAGYGS 598 Score = 40.9 bits (95), Expect = 3e-05 Identities = 74/369 (20%), Positives = 135/369 (36%), Gaps = 6/369 (1%) Query: 514 SDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA-- 571 S G+DS + S +G +ST +G S + SD + S + DS+ Sbjct: 390 STGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLI 449 Query: 572 ----SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSD 627 S + DS + S + SD + S S + +S+ + S + Sbjct: 450 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYG 509 Query: 628 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 687 S + S + ++SD + S S + ++S + S + +S + S Sbjct: 510 STLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQT 569 Query: 688 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 747 + SD + S + SDS + S + S + S + S + Sbjct: 570 AREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYG 629 Query: 748 SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSD 807 S S + +DS + S + +S + S ++ SD + S S + +DS Sbjct: 630 STSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLI 689 Query: 808 SESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD 867 + S + +S + S + SD SG S S++ +DS + GS + Sbjct: 690 AGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYH 749 Query: 868 SNSDSESGS 876 S+ + GS Sbjct: 750 SSLTAGYGS 758 Score = 40.9 bits (95), Expect = 3e-05 Identities = 71/378 (18%), Positives = 130/378 (34%), Gaps = 4/378 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G E + S G S + +DS +G ST +G +S+ + S SD Sbjct: 181 GSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQMAGYGSTQTGMKGSDL 240 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + S + S + DS+ + S + SD + S + + Sbjct: 241 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 300 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + +S + S + SD + S + DS + S Sbjct: 301 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 360 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + DS + S + SD + S + +DS + S + +S + Sbjct: 361 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGY 420 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD + S + DS + S + +S + S + SD Sbjct: 421 GSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 480 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSD----SDSTSDTGS 860 + S S + +S + S + S + GS + ++SD STS G+ Sbjct: 481 TAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGA 540 Query: 861 DNDSDSDSNSDSESGSNN 878 ++ + S + N+ Sbjct: 541 NSSLIAGYGSTQTASYNS 558 Score = 39.4 bits (91), Expect = 8e-05 Identities = 70/381 (18%), Positives = 126/381 (33%), Gaps = 14/381 (3%) Query: 510 IPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDS--------------ASDSDSASD 555 +P D D +SGS + + + ST S +S + Sbjct: 138 LPVTDDIDATIESGSTQPTQTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYG 197 Query: 556 SDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASD 615 S + +DS + S + +S + S SD + S + DS+ Sbjct: 198 STGTAGADSTLVAGYGSTQTAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLI 257 Query: 616 SDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 675 + S + DS + S + SD + S + +DS + S + + Sbjct: 258 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 317 Query: 676 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 735 S + S + SD + S + DS + S + DS + S Sbjct: 318 STQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQT 377 Query: 736 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSD 795 + SD + S + +DS + S + +S + S ++ SD + Sbjct: 378 AQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYG 437 Query: 796 SDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDST 855 S + DS + S + DS + S + SD +G S S++ +S Sbjct: 438 STGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLI 497 Query: 856 SDTGSDNDSDSDSNSDSESGS 876 + GS + S + GS Sbjct: 498 AGYGSTQTAGYGSTLTAGYGS 518 Score = 38.6 bits (89), Expect = 1e-04 Identities = 73/381 (19%), Positives = 139/381 (36%), Gaps = 4/381 (1%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + E+S G S + S +G ST +G DS+ + S + DS Sbjct: 405 GSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSL 464 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + SD + S S + +S+ + S + S + S + + Sbjct: 465 TAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQN 524 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 +SD + S S + ++S + S + +S + S + SD + S Sbjct: 525 ESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTG 584 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + SDS + S + S + S + S + S S + +DS + Sbjct: 585 TAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGY 644 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + +S + S + SD + S S + ++S + S + +S Sbjct: 645 GSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSIL 704 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS----DSDSTSDTGS 860 + S + SD S S S + +DS+ + GS +S S ST Sbjct: 705 TAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTARE 764 Query: 861 DNDSDSDSNSDSESGSNNNVV 881 + + S S +G++++++ Sbjct: 765 QSVLTTGYGSTSTAGADSSLI 785 Score = 37.4 bits (86), Expect = 4e-04 Identities = 70/375 (18%), Positives = 131/375 (34%), Gaps = 6/375 (1%) Query: 513 DSDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDS 570 S + GS + S +G ST +G+DS + S + +S + S Sbjct: 171 THQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQMAGYGS 230 Query: 571 ASDSDSASDSDSASDSDSASDSDSA----SDSDSASDSDSASDSDSASDSDSASDSDSDS 626 SD + S + DS+ S + DS+ + S + SD + Sbjct: 231 TQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTA 290 Query: 627 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 686 S + +DS + S + +S + S + SD + S + DS Sbjct: 291 GYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDS 350 Query: 687 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 746 + S + DS + S + SD + S + +DS + S + Sbjct: 351 SLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTA 410 Query: 747 DSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDS 806 +S + S + SD + S + DS + S + DS + S Sbjct: 411 GEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 470 Query: 807 DSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDS 866 + SD + S S + +S + S + S+ + ST +++D + Sbjct: 471 TQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLIT 530 Query: 867 DSNSDSESGSNNNVV 881 S S +G+N++++ Sbjct: 531 GYGSTSTAGANSSLI 545 Score = 37.0 bits (85), Expect = 4e-04 Identities = 75/372 (20%), Positives = 135/372 (36%), Gaps = 2/372 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + + SD G S + DS +G ST +G DS+ + S + SD Sbjct: 421 GSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 480 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S S + S + S + S + S + ++SD + S S + + Sbjct: 481 TAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGA 540 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 +S + S + +S + S + SD + S + SDS + S Sbjct: 541 NSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQ 600 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + S + S + S + S S + +DS + S + +S + Sbjct: 601 TASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGY 660 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD + S S + +DS + S + S + S + SD Sbjct: 661 GSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDL 720 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 S S S + +DS + S + S+ +G S ++ S T+ GS + + Sbjct: 721 TSGYGSTSTAGADSSLIAGYGSTQTASYHSS--LTAGYGSTQTAREQSVLTTGYGSTSTA 778 Query: 865 DSDSNSDSESGS 876 +DS+ + GS Sbjct: 779 GADSSLIAGYGS 790 Score = 37.0 bits (85), Expect = 4e-04 Identities = 75/373 (20%), Positives = 135/373 (36%), Gaps = 2/373 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + + SD G S S + +S +G ST +G S + S + ++SD Sbjct: 469 GSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDL 528 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S S + + S + S + +S + S + SD + S + S Sbjct: 529 ITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGS 588 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + S + S + S + S S + +DS + S Sbjct: 589 DSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQ 648 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + +S + S + SD + S S + +DS + S + +S + Sbjct: 649 TAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGY 708 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + SD S S S + +DS + S + S + S + S Sbjct: 709 GSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVL 768 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 + S S + +DS + S + S +G S ++ SD T+ GS + + Sbjct: 769 TTGYGSTSTAGADSSLIAGYGSTQTAGYHSI--LTAGYGSTQTAQERSDLTTGYGSTSTA 826 Query: 865 DSDSNSDSESGSN 877 +DS+ + GS Sbjct: 827 GADSSLIAGYGST 839 Score = 37.0 bits (85), Expect = 4e-04 Identities = 67/339 (19%), Positives = 124/339 (36%), Gaps = 2/339 (0%) Query: 514 SDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA 571 DS + GS + GSD +G STS +G +S+ + S + S + S Sbjct: 460 EDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGST 519 Query: 572 SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSD 631 + + SD + S S + ++S+ + S ++ +S + S + SD + Sbjct: 520 QTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAG 579 Query: 632 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 691 S + SDS + S + S + S + S + S S + +DS Sbjct: 580 YGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSS 639 Query: 692 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 751 + S + +S + S + SD + S S + +DS + S + Sbjct: 640 LIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAG 699 Query: 752 SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD 811 +S + S + SD S S S + ++S + S + S + S Sbjct: 700 YNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGST 759 Query: 812 SDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + S + S S + +DS+ + GS + S Sbjct: 760 QTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHS 798 Score = 33.6 bits (76), Expect = 0.005 Identities = 56/335 (16%), Positives = 107/335 (31%) Query: 527 NSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDS 586 S +D + + + S ++ D D+ +S S + Sbjct: 97 TKTSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPT 156 Query: 587 DSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDS 646 + + S S + S + +S + S + +DS + S Sbjct: 157 QTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQ 216 Query: 647 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 706 + +S + S SD + S + DS + S + DS + Sbjct: 217 TAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGY 276 Query: 707 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 766 S + SD + S + +DS + S + +S + S + SD Sbjct: 277 GSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDL 336 Query: 767 DSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSESDSDS 826 + S + DS + S + DS + S ++ SD + S + + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 827 DSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSD 861 DS + S +G +S ++ S T+ GSD Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSD 431 Score = 33.2 bits (75), Expect = 0.005 Identities = 66/339 (19%), Positives = 125/339 (36%), Gaps = 2/339 (0%) Query: 514 SDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA 571 S + GS + + SD +G STS +G++S+ + S ++ +S + S Sbjct: 508 YGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGST 567 Query: 572 SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSD 631 + SD + S + SDS+ + S ++ S + S + S + Sbjct: 568 QTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTG 627 Query: 632 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 691 S S + +DS + S + +S + S + SD + S S + +DS Sbjct: 628 YGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSS 687 Query: 692 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 751 + S + +S + S + SD S S S + +DS + S + Sbjct: 688 LIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTAS 747 Query: 752 SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD 811 S + S + S + S S + ++S + S + S + S Sbjct: 748 YHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGST 807 Query: 812 SDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + SD + S S + +DS+ + GS + +S Sbjct: 808 QTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNS 846 Score = 32.8 bits (74), Expect = 0.008 Identities = 74/372 (19%), Positives = 135/372 (36%), Gaps = 2/372 (0%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDS 564 G + +SD G S S + ++S +G ST + +S + S + SD Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDL 576 Query: 565 ASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDS 624 + S + S S + S + S+ + S + S + S S + + Sbjct: 577 TAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGA 636 Query: 625 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 684 DS + S + +S + S + SD + S S + +DS + S Sbjct: 637 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQ 696 Query: 685 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 744 + +S + S + SD S S S + +DS + S + S + Sbjct: 697 TAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGY 756 Query: 745 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDS 804 S + S + S S + +DS + S + S + S + SD Sbjct: 757 GSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDL 816 Query: 805 DSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDS 864 + S S + +DS + S + +S +G S ++ +SD T+ GS + + Sbjct: 817 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSI--LTAGYGSTQTAQENSDLTTGYGSTSTA 874 Query: 865 DSDSNSDSESGS 876 DS+ + GS Sbjct: 875 GYDSSLIAGYGS 886 Score = 32.8 bits (74), Expect = 0.008 Identities = 67/360 (18%), Positives = 131/360 (36%) Query: 522 SGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSD 581 +G S +G S + ++S + S S + ++S+ + S ++ +S + Sbjct: 506 AGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYG 565 Query: 582 SASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSD 641 S + SD + S + SDS+ + S ++ S + S + S Sbjct: 566 STQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLT 625 Query: 642 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 701 + S S + +DS + S + +S + S + SD + S S + +D Sbjct: 626 TGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGAD 685 Query: 702 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 761 S + S + +S + S + SD S S S + +DS + S Sbjct: 686 SSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQT 745 Query: 762 SDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSE 821 + S + S + S + S S + +DS + S + S + Sbjct: 746 ASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYG 805 Query: 822 SDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSDSNSDSESGSNNNVV 881 S + SD + S S + +DSS + ST G ++ + S + N+++ Sbjct: 806 STQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLT 865 Score = 31.6 bits (71), Expect = 0.018 Identities = 77/387 (19%), Positives = 138/387 (35%), Gaps = 14/387 (3%) Query: 505 GEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSA--------------SDS 550 G + E SD G S + SDS +G ST + S+ S Sbjct: 565 GSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVL 624 Query: 551 DSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDS 610 + S S + +DS+ + S + +S + S + SD + S S + + Sbjct: 625 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGA 684 Query: 611 DSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 670 DS+ + S + +S + S + SD S S S + +DS + S Sbjct: 685 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQ 744 Query: 671 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS 730 + S + S + S + S S + +DS + S + S + Sbjct: 745 TASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGY 804 Query: 731 DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDS 790 S + SD + S S + +DS + S + +S + S ++ +SD Sbjct: 805 GSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDL 864 Query: 791 DSDSDSDSDSDSDSDSDSESDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + S S + DS + S + +S + S + SD +G S S++ Sbjct: 865 TTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 924 Query: 851 DSDSTSDTGSDNDSDSDSNSDSESGSN 877 +S + GS + S + GS+ Sbjct: 925 ESSLIAGYGSTQTASFKSTLMAGYGSS 951 Score = 31.3 bits (70), Expect = 0.026 Identities = 68/339 (20%), Positives = 125/339 (36%), Gaps = 2/339 (0%) Query: 514 SDSDPGSDSGSDSNSDSGSD--SGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSA 571 +S + GS + GSD +G ST +GSDS+ + S ++ S + S Sbjct: 556 YNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGST 615 Query: 572 SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSD 631 + S + S S + +DS+ + S + +S + S + SD + Sbjct: 616 QTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAG 675 Query: 632 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 691 S S + +DS + S + +S + S + SD S S S + +DS Sbjct: 676 YGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSS 735 Query: 692 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 751 + S + S + S + S + S S + +DS + S + Sbjct: 736 LIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAG 795 Query: 752 SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESD 811 S + S + SD + S S + ++S + S + +S + S Sbjct: 796 YHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGST 855 Query: 812 SDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDS 850 + +SD + S S + DS+ + GS + +S Sbjct: 856 QTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNS 894 Score = 31.3 bits (70), Expect = 0.026 Identities = 67/360 (18%), Positives = 128/360 (35%) Query: 522 SGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSD 581 + +S +G S + S + S + SDS+ + S ++ S + Sbjct: 554 ASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYG 613 Query: 582 SASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSD 641 S + S + S S + +DS+ + S + +S + S + SD Sbjct: 614 STQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLT 673 Query: 642 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 701 + S S + +DS + S + +S + S + SD S S S + +D Sbjct: 674 AGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGAD 733 Query: 702 SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD 761 S + S + S + S + S + S S + +DS + S Sbjct: 734 SSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQT 793 Query: 762 SDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSE 821 + S + S + SD + S S + +DS + S + +S + Sbjct: 794 AGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYG 853 Query: 822 SDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSDSNSDSESGSNNNVV 881 S + +SD + S S + DSS + ST G ++ + S + N+++ Sbjct: 854 STQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLT 913
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006200	IGASERPTASE	31	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.8 bits (69), Expect = 0.016 Identities = 28/162 (17%), Positives = 52/162 (32%), Gaps = 4/162 (2%) Query: 261 ALKLKADTEAAKNDVSKRSKRSLNTQNNKST-TQEISEEQKAEYQRKSEALKERFINRQK 319 +KA+T+ + S + T K T T E E+ K E ++ E K Sbjct: 1073 KSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVT-SQVSP 1131 Query: 320 SKNESVVSLIDDEDDNENDRQLVVSAPSKKPTTPTTYTETTTQVPMPTVERQTQQQIVYK 379 + +S E END + + P + T + + + T+ V Sbjct: 1132 KQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNT 1191 Query: 380 TPKPLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALP 421 + N E+ TT + + + ++P Sbjct: 1192 GNSVVE--NPENTTPATTQPTVNSESSNKPKNRHRRSVRSVP 1231
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1006240	PF05704	28	0.035	Capsular polysaccharide synthesis protein
>PF05704#Capsular polysaccharide synthesis protein Length = 307 Score = 27.5 bits (61), Expect = 0.035 Identities = 13/69 (18%), Positives = 24/69 (34%), Gaps = 7/69 (10%) Query: 116 EWVKKNYENTNHRYLVTLNLNSK-------KFTYCTKIIYQAYKFGVSEKSVKSYGLHII 168 W + Y N + +++ N + + YK + +Y HI Sbjct: 239 YWKEIPYVNNVNPHMLQYLGNLPYDNSMFNYIKSTSPVQKLTYKLDYNNLKRNTYYDHIF 298 Query: 169 SPYAIKDNF 177 S +KDN+ Sbjct: 299 SIDKLKDNY 307

SAI8T7_1007990

SAI8T7_1008060

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1007990	4	14	-1.048854	Phosphopantetheine adenylyltransferase
SAI8T7_1008000	5	12	-1.600947	UPF0348 protein NWMN_0989
SAI8T7_1008010	4	12	-1.702031	Putative uncharacterized protein
SAI8T7_1008020	4	13	-1.754714	Iron-regulated surface determinant protein B
SAI8T7_1008030	2	13	-2.757987	Iron-regulated surface determinant protein A
SAI8T7_1008040	-1	14	-2.668950	Iron-regulated surface determinant protein C
SAI8T7_1008050	-2	13	-2.067117	Putative uncharacterized protein
SAI8T7_1008060	-1	12	-0.398815	High-affinity heme uptake system protein isdE

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1007990	LPSBIOSNTHSS	219	1e-76	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 219 bits (560), Expect = 1e-76 Identities = 77/155 (49%), Positives = 112/155 (72%) Query: 5 IAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKEGTFSLEERMDLIEQSVKHLP 64 A+ PGSFDPIT+GHLDIIER FD+++V VL+N K+ FS++ER++ I +++ HLP Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61 Query: 65 NVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELRLTSMNKKLNNEIETLYMMSSTN 124 N +V F GL V+Y Q A I+RGLR +SDFE EL++ + NK L +++ET+++ +ST Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121 Query: 125 YSFISSSIVKEVAAYRADISEFVPPYVEKALKKKF 159 YSF+SSS+VKEVA + ++ FVP +V AL +F Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHVAAALYDQF 156
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008020	IGASERPTASE	36	6e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 35.8 bits (82), Expect = 6e-04 Identities = 37/194 (19%), Positives = 71/194 (36%), Gaps = 15/194 (7%) Query: 447 RIVDKEAFTKANTDKSNKKEQQDNSAKKEA---------TPATPSKPTPSPVEKESQKQD 497 + VD T N +++ N+ + PATPS+ T + E Q+ Sbjct: 990 QTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESK 1049 Query: 498 SQKDDNKQLPSVEKENDASSESGKDKTPATKPT------KGEVESSSTTPTKVVSTTQNV 551 + + + + +N ++ K A T E + + TT TK +T + Sbjct: 1050 TVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKE 1109 Query: 552 AKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLP 611 K + KT + TS S + + S +Q + T + +Q N Sbjct: 1110 EKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTE 1169 Query: 612 QTGEESNKDMTLPL 625 Q +E++ ++ P+ Sbjct: 1170 QPAKETSSNVEQPV 1183 Score = 30.0 bits (67), Expect = 0.035 Identities = 27/156 (17%), Positives = 45/156 (28%), Gaps = 5/156 (3%) Query: 37 EAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAPET-KPVANAVSVSNKEVEAPTSETKE 95 A EE TE + V S + ++ ET +P A ++ V +++ Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQT 1162 Query: 96 AKEVKEVKAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEM 155 + KET + + T N + NP + P Sbjct: 1163 NTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVE----NPENTTPATTQPTVNSESSNK 1218 Query: 156 KKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQS 191 K + +V+PA D L S Sbjct: 1219 PKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTS 1254
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008030	IGASERPTASE	34	0.001	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/132 (20%), Positives = 44/132 (33%), Gaps = 4/132 (3%) Query: 184 ADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTT 243 + A+ + P PA P TE + K + + +V + Sbjct: 1015 EEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKS 1074 Query: 244 KDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNET 303 + TQT + AQ+ E + QT + V K+Q+ KVT + Sbjct: 1075 NVKANTQTN---EVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS-QVS 1130 Query: 304 PKQASKAKELPK 315 PKQ P+ Sbjct: 1131 PKQEQSETVQPQ 1142
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008060	FERRIBNDNGPP	45	2e-07	Ferrichrome-binding periplasmic protein signature.
>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature. Length = 296 Score = 44.6 bits (105), Expect = 2e-07 Identities = 34/209 (16%), Positives = 79/209 (37%), Gaps = 11/209 (5%) Query: 55 PNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDS--L 112 P V ++G EPN+E + ++KP+ ++ + + + +G+ + L Sbjct: 72 PPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPL 131 Query: 113 KGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVA 172 +KS+T++ D N ++ A+ + ++ + K+ P +L + P LV Sbjct: 132 AMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVF 191 Query: 173 TDKSYIGDLVKIAGGENVIKVKDRQYISSNT---ENLLNINPDIILRLPHGMPEEVKKMF 229 S +++ G N + + + S + L +L H +++ + Sbjct: 192 GPNSLFQEILDEYGIPNAWQ-GETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDAL- 249 Query: 230 QKEFKQNDIWKHFKAVKNNHVYDLEEVPF 258 +W+ V+ + V F Sbjct: 250 ----MATPLWQAMPFVRAGRFQRVPAVWF 274

SAI8T7_1008260

SAI8T7_1008310

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1008260	0	13	-1.494506	Alpha-hemolysin
SAI8T7_1008270	1	11	-0.822916	Superantigen-like protein
SAI8T7_1008280	1	11	-0.710644	SA1010 protein
SAI8T7_1008290	1	11	-0.559013	Putative uncharacterized protein
SAI8T7_1008300	0	11	-0.487684	Ornithine carbamoyltransferase
SAI8T7_1008310	0	14	-0.204395	Carbamate kinase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008260	BICOMPNTOXIN	314	e-109	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 314 bits (805), Expect = e-109 Identities = 72/318 (22%), Positives = 144/318 (45%), Gaps = 24/318 (7%) Query: 12 VTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNTTVKTGDLVTYDKEN--GMHKKVFY 69 +TTTL + L+ P+AN + T DIG + ++ N G+ + + + Sbjct: 7 LTTTLSVS--LLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQF 64 Query: 70 SFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS-GLAWPSAFKVQLQLPDNEVAQIS 128 F+ DK +NK L+++ +G I+ + Y+ + N + WP + + L+ D V+ I Sbjct: 65 DFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLI- 123 Query: 129 DYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILES 188 +Y P+N I++ TL Y GN + +GG N S ++ Y Q ++ + +E Sbjct: 124 NYLPKNKIESTNVSQTLGYNIGGNFQSAPS--LGGNGSFNYS--KSISYTQQNYVSEVEQ 179 Query: 189 PTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAAENFLDPNKASSL 248 K V W V N+ ++ + + LF+ + S + F+ ++ L Sbjct: 180 QNSKSVLWGVKANSFATESG-------QKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPL 232 Query: 249 LSSGFSPDFATVITMDRKASKQQTNIDVIYERVRD-----DYQLHWTSTNWKGTNTKDKW 303 + SGF+P F ++ + K S + ++ Y R D H+ ++ G + + Sbjct: 233 VQSGFNPSFIATVSHE-KGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAF 291 Query: 304 TDRS-SERYKIDWEKEEM 320 +R+ + +Y+++W+ E+ Sbjct: 292 VNRNYTVKYEVNWKTHEI 309
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008270	TOXICSSTOXIN	48	6e-09	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 48.5 bits (115), Expect = 6e-09 Identities = 53/223 (23%), Positives = 89/223 (39%), Gaps = 12/223 (5%) Query: 34 MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQDETNINELIKYYTQPHF 93 M+K + N + + LLL T P+ S A + D NI +L+ +Y+ Sbjct: 1 MNKKLLMNFFIVSPLLLATTATDFTPVPLSSNQIIKTAKASTND--NIKDLLDWYSSGSD 58 Query: 94 SFSNKWLYQYDNENIYVELKRYSWSAHISLWGAESWGNINQLKDRYVDVFGLKD-KDTDQ 152 +F+N DN + +K S + ++ + + + K VD+ + K Sbjct: 59 TFTN--SEVLDNSLGSMRIKNTDGSISLIIFPSP-YYSPAFTKGEKVDLNTKRTKKSQHT 115 Query: 153 LWWSYRETFTGGVTPAAK-PSDKTYNLFVQYKDKLQTIIGAHKIYQGNKPVLTLKEIDFR 211 +Y GVT K P+ L V+ K + K +K L + +DF Sbjct: 116 SEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYGPKF---DKKQLAISTLDFE 172 Query: 212 AREALIKNKILY-TENRNKGKLKIT-GGGNNYTIDLSKRLHSD 252 R L + LY + ++ G KIT G+ Y DLSK+ + Sbjct: 173 IRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYN 215
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008280	TOXICSSTOXIN	57	7e-12	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 56.6 bits (136), Expect = 7e-12 Identities = 55/228 (24%), Positives = 91/228 (39%), Gaps = 15/228 (6%) Query: 16 LLLGTAFTQFPNTPINSSSEAKAYYINQNETNVNELTKYYSQKYLTFSNSTLWQKDNGTI 75 LLL T T F P++S+ K + N+ N+ +L +YS TF+NS + G++ Sbjct: 15 LLLATTATDFTPVPLSSNQIIKTAKASTND-NIKDLLDWYSSGSDTFTNSEVLDNSLGSM 73 Query: 76 HATLLQFSWYSHIQVYGPESWGNINQLRNKSVDIFGI---KDQETIDSFALSQETFTGGV 132 ++ + S + P + + + + VD+ K Q T + + + GV Sbjct: 74 R---IKNTDGSISLIIFPSPYYSPAFTKGEKVDLNTKRTKKSQHTSEGTYIHFQI--SGV 128 Query: 133 TPA-ATSNDKHYKLNVTYKDKAETFTGGFPVYEGNKPVLTLKELDFRIRQTLIKSKKLYN 191 T L V K + + + +K L + LDF IR L + LY Sbjct: 129 TNTEKLPTPIELPLKV--KVHGKDSPLKYG-PKFDKKQLAISTLDFEIRHQLTQIHGLYR 185 Query: 192 NSYNKGQI-KITGTDNN-YTIDLSKRLPSTDANRYVKKPQNAKIEVIL 237 +S G KIT D + Y DLSK+ + + IE + Sbjct: 186 SSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEKPPINIDEIKTIEAEI 233
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008290	TOXICSSTOXIN	62	1e-13	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 61.6 bits (149), Expect = 1e-13 Identities = 62/222 (27%), Positives = 96/222 (43%), Gaps = 17/222 (7%) Query: 2 KKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISENETNINELIKYYTQPHFS 61 KK +MN ++S LLL TT+T P+S + K S N+ NI +L+ +Y+ + Sbjct: 3 KKLLMNFFIVSP--LLLATTATDFTPVPLSSNQIIKTAKASTND-NIKDLLDWYSSGSDT 59 Query: 62 LSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQLRNKYVDIFGT---KDEDTV 118 + + GS+ ++ + +F S + + + + VD+ K + T Sbjct: 60 FTNSEVLDNSLGSMR--IKNTDGSISLIIFPSP-YYSPAFTKGEKVDLNTKRTKKSQHTS 116 Query: 119 EGYWTYDETFTGGVTPA-ATSSDKPYRLFLKYSDKQQTIIGGHEFYKGNKPVLTLKELDF 177 EG TY GVT + L +K K + G +F +K L + LDF Sbjct: 117 EG--TYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYGPKF---DKKQLAISTLDF 171 Query: 178 RIRQTLIKNKKLYNGEFNKGQI-KIT-ADGNNYTIDLSKKLK 217 IR L + LY G KIT DG+ Y DLSKK + Sbjct: 172 EIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFE 213
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1008310	CARBMTKINASE	389	e-139	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 389 bits (1001), Expect = e-139 Identities = 145/314 (46%), Positives = 211/314 (67%), Gaps = 7/314 (2%) Query: 1 MMAKIVVALGGNALGK-----SPQEQLELVKNTAKSLVGLITKGHEIVISHGNGPQVGSI 55 M ++V+ALGGNAL + S +E ++ V+ TA+ + +I +G+E+VI+HGNGPQVGS+ Sbjct: 1 MGKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSL 60 Query: 56 NLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDKQVVTLVTQVEVD 115 L ++ PA P GAMSQ +IGY +Q++L+NEL GM+K+VVT++TQ VD Sbjct: 61 LLHMDAGQATYGIPAQPMDVAGAMSQGWIGYMIQQALKNELRKRGMEKKVVTIITQTIVD 120 Query: 116 ENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVPSPQPISIIELESIK 175 +NDPAF NP+KP+G FY++E A+++ +EKG+I ED+GRG+RRVVPSP P +E E+IK Sbjct: 121 KNDPAFQNPTKPVGPFYDEETAKRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETIK 180 Query: 176 TLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLGANIQCDQLIILTAIDY 235 L++ +VIA+GGGG+PVI E KG++AVIDKD L + D +ILT ++ Sbjct: 181 KLVERGVIVIASGGGGVPVILE-DGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNG 239 Query: 236 VYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAAISFIENNPKGSVLITSLN 295 + + TE +Q L+ V+EL++Y +E F GSM PK+ AAI FIE + ++ I L Sbjct: 240 AALYYGTEKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWGGERAI-IAHLE 298 Query: 296 ELDAALEGKVGTVI 309 + ALEGK GT + Sbjct: 299 KAVEALEGKTGTQV 312

SAI8T7_1011200

SAI8T7_1011290

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1011200	0	13	-3.450553	Similar to DNA transport mechinery protein
SAI8T7_1011210	0	10	-2.031559	Similar to late competence protein comGA
SAI8T7_1011220	0	9	-1.725568	Similar to metallo-beta-lactamase superfamily
SAI8T7_1011230	0	9	-1.779948	Glucokinase
SAI8T7_1011240	-1	10	-2.387884	Putative uncharacterized protein
SAI8T7_1011250	-2	10	-1.161970	5-formyltetrahydrofolate cyclo-ligase
SAI8T7_1011260	-2	10	-0.917319	Penicillin-binding protein 3
SAI8T7_1011270	-1	11	-1.515539	Superoxide dismutase [Mn/Fe] 1
SAI8T7_1011280	-2	11	-1.834325	ABC-3 protein
SAI8T7_1011290	-2	12	-1.488081	ABC transporter ATP-binding protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011200	BCTERIALGSPF	81	2e-19	Bacterial general secretion pathway protein F signa...
>BCTERIALGSPF#Bacterial general secretion pathway protein F signature. Length = 408 Score = 81.4 bits (201), Expect = 2e-19 Identities = 51/265 (19%), Positives = 109/265 (41%), Gaps = 3/265 (1%) Query: 43 ERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLY 102 E G++ VL +Y + ++ R+ + + YP +L + IA++ IL V+P+ + + Sbjct: 143 ETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLTVVAIAVVSILLSVVVPKVVEQF 202 Query: 103 TSMNIQLSSFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKIN-FVMKLP 161 M L + L ++ T ML+ + + +++ + ++ LP Sbjct: 203 IHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMAFRVMLRQEKRRVSFHRRLLHLP 262 Query: 162 LISGYFQLFKTYFVTNELVLFYKNGITLQSIVDVYINHSS-DPFRQFLGKYLLTYSEMGY 220 LI + T L + + + L + + + S D R L E G Sbjct: 263 LIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDVMSNDYARHRLSLATDAVRE-GV 321 Query: 221 GLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILF 280 L + LE+ F P + + GE+ G+L+ L+ + ++ + +P+L Sbjct: 322 SLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADNQDREFSSQMTLALGLFEPLLV 381 Query: 281 LILGLFIVAIYLVIMLPMFQMMQSI 305 + + ++ I L I+ P+ Q+ + Sbjct: 382 VSMAAVVLFIVLAILQPILQLNTLM 406
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011220	SHIGARICIN	27	0.039	Ribosome inactivating protein family signature.
>SHIGARICIN#Ribosome inactivating protein family signature. Length = 289 Score = 27.5 bits (61), Expect = 0.039 Identities = 20/99 (20%), Positives = 38/99 (38%), Gaps = 11/99 (11%) Query: 82 DFLKDPVKNGADKFKQYGLPIITSKVTPEK-------LNEGSTEIE-GFKFNVLHTPGHS 133 F+ + K + K Y +P++ S + + N I ++ G+ Sbjct: 39 VFISNLRKALPYERKLYDIPLLRSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYR 98 Query: 134 PGSLTYVFDEFAVVG--DTLFNNGIGRTDL-YKGDYETL 169 G +Y F+E + +F + + L Y G+YE L Sbjct: 99 AGDTSYFFNEASATEAAKYVFKDAKRKVTLPYSGNYERL 137
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011230	PF03309	30	0.012	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 29.7 bits (67), Expect = 0.012 Identities = 32/154 (20%), Positives = 51/154 (33%), Gaps = 37/154 (24%) Query: 5 ILAADVGGTTCKLGIFTPELEQ---LHKWSIHTD---TSDSTGYTLLKGIYDSFVEKVNE 58 +LA DV T +G+ + + + +W I T+ T+D + G+ Sbjct: 2 LLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELA-LTIDGLI--------- 51 Query: 59 NNYNFSNVLGVGIG--VPGPVDFEKGTVNGAVNLYWPE------KVNVREIFEQFVDCPV 110 + + G VP V E V + YWP + VR VD P Sbjct: 52 -GDDAERLTGASGLSTVP-SVLHE---VRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPK 106 Query: 111 YVDND--ANIAALGEKHKGAGEGADDVVAITLGT 142 V D N A K+ + + G+ Sbjct: 107 EVGADRIVNCLAAYHKYGT------AAIVVDFGS 134
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011240	TCRTETA	33	0.002	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 33.3 bits (76), Expect = 0.002 Identities = 29/170 (17%), Positives = 54/170 (31%), Gaps = 51/170 (30%) Query: 241 MLTVYFIAGLFGN--------FVSLSFNTTTISVGASGAIFGLIGSIFAMMY---VSKTF 289 ++ V+FI L G F F+ ++G S A FG++ S+ M V+ Sbjct: 215 LMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARL 274 Query: 290 NKK----------MLGQLLIA-----------LVILVGVSLFMS------NINIVAHIGG 322 ++ G +L+A +V+L + M + + G Sbjct: 275 GERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG 334 Query: 323 FIGGLLITL-----------IGYYYKVNRNIF--WILLIGMLVIFIALQI 359 + G L L Y + + W + G + + L Sbjct: 335 QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPA 384
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1011290	PF05272	33	0.001	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 33.1 bits (75), Expect = 0.001 Identities = 13/43 (30%), Positives = 20/43 (46%), Gaps = 7/43 (16%) Query: 39 LAIVGPNGAGKSTLLKLILGLLPLQSGEIFVEG-IDFKNKKTS 80 + + G G GKSTL+ ++GL + F + D K S Sbjct: 599 VVLEGTGGIGKSTLINTLVGL------DFFSDTHFDIGTGKDS 635

SAI8T7_1013280

SAI8T7_1013430

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1013280	1	10	-2.442092	Trypsin
SAI8T7_1013290	1	11	-2.749488	Serine protease splC
SAI8T7_1013300	2	10	-2.682918	Glutamyl endopeptidase
SAI8T7_1013310	3	12	-3.902729	Serine protease splA
SAI8T7_1013320	4	13	-4.308101	Putative Probable beta-lactamase
SAI8T7_1013330	4	14	-4.573290	Putative uncharacterized protein
SAI8T7_1013340	5	16	-4.607500	Leukotoxin, LukD
SAI8T7_1013350	7	18	-5.810955	Leukotoxin S-subunit
SAI8T7_1013360	9	20	-7.177831	Putative uncharacterized protein
SAI8T7_1013370	10	20	-6.561952	Putative uncharacterized protein
SAI8T7_1013380	8	19	-7.051920	Protein of hypothetical function DUF1828
SAI8T7_1013390	7	19	-6.235395	Enterotoxin type G
SAI8T7_1013400	4	15	-4.069391	Enterotoxin SeN
SAI8T7_1013410	2	12	-2.749384	Extracellular enterotoxin type I
SAI8T7_1013420	1	12	-1.335346	Enterotoxin
SAI8T7_1013430	1	12	-0.894221	Enterotoxin

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013280	V8PROTEASE	112	2e-31	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 112 bits (280), Expect = 2e-31 Identities = 58/227 (25%), Positives = 100/227 (44%), Gaps = 26/227 (11%) Query: 38 IQQTAKA-----ENSVKLITNTNVAPYSGVTWMGA--------GTGFVVGNHTIITNKHV 84 ++Q A N IT+T Y+ VT++ +G VVG T++TNKHV Sbjct: 61 LEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHV 120 Query: 85 TYHM-KVGDEIKAHPNGFY--NNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFK 141 +KA P+ N G + +I Y G+ D+A+V+ + + Sbjct: 121 VDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSP---NEQNKHIG 177 Query: 142 DFTSKFNIA--SEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVTSDAVVQPGS 199 + ++ +E + N+ I+V GYP + M+ES GK+ + G + D G+ Sbjct: 178 EVVKPATMSNNAETQVNQNITVTGYP-GDKPVATMWESKGKITYLKGEAMQYDLSTTGGN 236 Query: 200 SGSPILNSKREAIGVMYASDKPTGESTRSFAVYFSPEIKKFIADNLD 246 SGSP+ N K E IG+ + AV+ + ++ F+ N++ Sbjct: 237 SGSPVFNEKNEVIGIHWGGVPNEFNG----AVFINENVRNFLKQNIE 279
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013290	V8PROTEASE	177	1e-56	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 177 bits (451), Expect = 1e-56 Identities = 64/217 (29%), Positives = 106/217 (48%), Gaps = 23/217 (10%) Query: 37 EKNVTQVKDTNNFPYNGVVSFK--------NATGFVIGKNTIITNKHV-SKDYKVGDRIT 87 + Q+ DT N Y V + A+G V+GK+T++TNKHV + + Sbjct: 73 NNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALK 132 Query: 88 AHP---NGDKGNGGIYKIKSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAK 144 A P N D G + + I+ Y G+ D++++ + + E V+ + Sbjct: 133 AFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNK-----HIGEVVKPATMSN 187 Query: 145 DA--KVDDKIKVIGYPLPAQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNN 202 +A +V+ I V GYP +ES G I +K + +D GNSGSPV N N Sbjct: 188 NAETQVNQNITVTGYPGDK-PVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKN 246 Query: 203 EVIGVVYGGIGKIGSEYNGAVYFTPQIKDFIQKHIEQ 239 EVIG+ +GG + +E+NGAV+ +++F++++IE Sbjct: 247 EVIGIHWGG---VPNEFNGAVFINENVRNFLKQNIED 280
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013300	V8PROTEASE	182	4e-58	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 182 bits (462), Expect = 4e-58 Identities = 65/230 (28%), Positives = 108/230 (46%), Gaps = 29/230 (12%) Query: 43 EVQQTAKA-----ENNVTKIQDTNIFPYTGVVAFKS--------ATGFVVGKNTILTNKH 89 ++Q A N+ +I DT Y V + A+G VVGK+T+LTNKH Sbjct: 60 PLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKH 119 Query: 90 V-SKNYKVGDRITAHP---NSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGF 145 V + + A P N D G ++ ++I Y G+ D+++++ + Sbjct: 120 VVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNK----- 174 Query: 146 NFNDNVTPFKYAAGA--KAGERIKVIGYPHPYKNKYVLYESTGPVMSVEGSSIVYSAHTE 203 + + V P + A + + I V GYP K ++ES G + ++G ++ Y T Sbjct: 175 HIGEVVKPATMSNNAETQVNQNITVTGYPGD-KPVATMWESKGKITYLKGEAMQYDLSTT 233 Query: 204 SGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENID 253 GNSGSPV N NE++GIH+ V N+ N V+ ++ F+ +NI+ Sbjct: 234 GGNSGSPVFNEKNEVIGIHWGG-VPNEFNG---AVFINENVRNFLKQNIE 279
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013310	V8PROTEASE	138	1e-41	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 138 bits (349), Expect = 1e-41 Identities = 63/212 (29%), Positives = 100/212 (47%), Gaps = 18/212 (8%) Query: 36 EKNVKEITDATKAPYNSVVAFA--------GGTGVVVGKNTIVTNKHIAKSNDIFKNRVA 87 + +ITD T Y V +GVVVGK+T++TNKH+ + + + Sbjct: 73 NNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALK 132 Query: 88 AHYS---SKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGA-- 142 A S G + + I +Y G+ DLAIV + + + + V + A Sbjct: 133 AFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVK-FSPNEQNKHIGEVVKPATMSNNAET 191 Query: 143 KAKDRISVIGYPKGAQTKYKMFESTGTINHISGTFIEFDAYAQPCNSGSPVLNSKHELIG 202 + I+V GYP G + M+ES G I ++ G +++D NSGSPV N K+E+IG Sbjct: 192 QVNQNITVTGYP-GDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIG 250 Query: 203 ILYAGSGKDESEKNFGVYFTPQLKEFIQNNIE 234 I + G +E N V+ ++ F++ NIE Sbjct: 251 IHWGGVP---NEFNGAVFINENVRNFLKQNIE 279
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013340	BICOMPNTOXIN	396	e-141	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 396 bits (1020), Expect = e-141 Identities = 96/329 (29%), Positives = 177/329 (53%), Gaps = 24/329 (7%) Query: 1 MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQ 60 M K++ ++++ S+ L + ++ A+ + I + K T ++K ++Q Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 61 ILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQ-FYWGGKYNVSVSSESNDA 119 + F+F+KDK Y+KD L+LK G I+S N K N+ + W +YN+ + + + Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTN-DKY 119 Query: 120 VNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTI 179 V++++Y PKN+ E V QTLGY+ GG+ + L G NGS ++S++I+Y Q++Y + + Sbjct: 120 VSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGG--NGSFNYSKSISYTQQNYVSEV 177 Query: 180 DRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQM 239 +++ N KS+ WGV+A+ + ++LF+G + S + F+P ++ Sbjct: 178 EQQ-NSKSVLWGVKANSFAT-------ESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSEL 229 Query: 240 PLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD---------RYTNQWNRLHWIGN 290 P L + FNP FI+ +SH++ + S+ ++TY R MD Y N + H + N Sbjct: 230 PPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHN 289 Query: 291 NYKNQNTVTFTSTYEVDWQNHTVKLIGTD 319 + N+N +T YEV+W+ H +K+ G + Sbjct: 290 AFVNRN---YTVKYEVNWKTHEIKVKGQN 315
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013350	BICOMPNTOXIN	433	e-156	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 433 bits (1116), Expect = e-156 Identities = 214/318 (67%), Positives = 256/318 (80%), Gaps = 10/318 (3%) Query: 1 MFKKKMLAATLSVGLIAPLASPIQE-SRANTNIENIGDGA--EVIKRTEDVSSKKWGVTQ 57 M K K+L TLSV L+APLA+P+ E ++A + E+IG G+ E+IKRTED +S KWGVTQ Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 58 NVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIWPFQYNIGLTTKDP 117 N+QFDFVKDKKYNKDALI+KMQGFI+SRT++ + K + + K M WPFQYNIGL T D Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNH--VKAMRWPFQYNIGLKTNDK 118 Query: 118 NVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYSKTISYTQKSYVSEVD 177 VSLINYLPKNKIE+T+V QTLGYNIGGNFQSAPS+GGNGSFNYSK+ISYTQ++YVSEV+ Sbjct: 119 YVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVE 178 Query: 178 KQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAREYFAPDNQLPPLVQSGF 237 +QNSKSV WGVKAN F T G+KSA D LFV + R+YF PD++LPPLVQSGF Sbjct: 179 QQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPH-SKDPRDYFVPDSELPPLVQSGF 237 Query: 238 NPSFITTLSHEKGSSDTSEFEISYGRNLDITYA----TLFPRTGIYAERKHNAFVNRNFV 293 NPSFI T+SHEKGSSDTSEFEI+YGRN+D+T+A T + + + R HNAFVNRN+ Sbjct: 238 NPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYT 297 Query: 294 VRYEVNWKTHEIKVKGHN 311 V+YEVNWKTHEIKVKG N Sbjct: 298 VKYEVNWKTHEIKVKGQN 315
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013390	BACTRLTOXIN	195	4e-64	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 195 bits (497), Expect = 4e-64 Identities = 109/261 (41%), Positives = 155/261 (59%), Gaps = 11/261 (4%) Query: 4 LSTVIIILILEIVFHNMN-YVNAQPDPKLDELNKVSDYKNNKGTMGNVMNLYTSPPVEGR 62 +S VI+I L +V N +QPDP D+L+K S++ GTMGN+ LY V Sbjct: 7 ISRVILIFALILVISTPNVLAESQPDPMPDDLHKSSEFT---GTMGNMKYLYDDHYVSAT 63 Query: 63 GVINSRQFLSHDLIFPI---EYKSYNEVKTELENTELANNYKDKKVDIFGVPYFYTCIIP 119 V + +FL+HDLI+ I + K+Y++VKTEL N +LA YKD+ VD++G Y+ C Sbjct: 64 KVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNCYFS 123 Query: 120 KSEPDINQNFGGCCMYGGLTF---NSSENERDKLITVQVTIDNRQSLGFTITTNKNMVTI 176 + G CMYGG+T N +N + + V+V + R ++ F + T+K VT Sbjct: 124 SKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENKRNTISFEVQTDKKSVTA 183 Query: 177 QELDYKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWFDLFPKKELVPFVPYKFLN 236 QELD KAR++L +K LYEF+ S +E+GYIKF E N +FW+D+ P F K+L Sbjct: 184 QELDIKARNFLINKKNLYEFNSSPYETGYIKFIENNGNTFWYDMMPAPGDK-FDQSKYLM 242 Query: 237 IYGDNKVVDSKSIKMEVFLNT 257 +Y DNK VDSKS+K+EV L T Sbjct: 243 MYNDNKTVDSKSVKIEVHLTT 263
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013400	BACTRLTOXIN	155	9e-49	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 155 bits (394), Expect = 9e-49 Identities = 76/265 (28%), Positives = 124/265 (46%), Gaps = 21/265 (7%) Query: 2 RLFYIAAIII-TLLCLINNNYVNAEV----DKKDLKKKSDLDSSKLFNLTSYYTDITWQL 56 RLF I+I L+ +I+ V AE DL K S+ + + N+ Y D + Sbjct: 4 RLFISRVILIFALILVISTPNVLAESQPDPMPDDLHKSSEF-TGTMGNMKYLYDDH--YV 60 Query: 57 DESNKISTDQLLNNTIILKNIDISVLKTSSLKVEFNSSDLANQFKGKNIDIYGLYFGNKC 116 + S D+ L + +I D + +K E + DLA ++K + +D+YG + C Sbjct: 61 SATKVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNC 120 Query: 117 -------VGLTEEKTSCLYGGVTIHDGNQLDEEKV--IGVNVFKDGVQQEGFVIKTKKAK 167 VG +C+YGG+T H+GN D + + V V+++ F ++T K Sbjct: 121 YFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENKRNTISFEVQTDKKS 180 Query: 168 VTVQELDTKVRFKLENLYKIYNKDTGNIQKGCIFFHSHNHQDQSFYYDLYNVKGSVG--A 225 VT QELD K R L N +Y ++ + G I F +N +F+YD+ G + Sbjct: 181 VTAQELDIKARNFLINKKNLYEFNSSPYETGYIKFIENN--GNTFWYDMMPAPGDKFDQS 238 Query: 226 EFFQFYSDNRTVSSSNYHIDVFLYK 250 ++ Y+DN+TV S + I+V L Sbjct: 239 KYLMMYNDNKTVDSKSVKIEVHLTT 263
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013410	BACTRLTOXIN	108	2e-30	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 108 bits (270), Expect = 2e-30 Identities = 54/227 (23%), Positives = 98/227 (43%), Gaps = 37/227 (16%) Query: 30 VGNLRNFYTKHDYIDLKGVTDKNLPIANQLEFS------TGTNDLISESNNWDEISKFKG 83 +GN++ Y H K + +A+ L ++ + + +E N D K+K Sbjct: 48 MGNMKYLYDDHYVSATKVKSVDKF-LAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKD 106 Query: 84 KKLDIFGIDY-------------NGPCKSKYMYGGATL-SGQYLNSARKIPINLWVNGKH 129 + +D++G +Y MYGG T G + ++ + + V Sbjct: 107 EVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENK 166 Query: 130 KTISTDKIATNKKLVTAQEIDVKLRRYLQEEYNIYGHNNTGKGKEYGYKSKFYSGFNNGK 189 + + ++ T+KK VTAQE+D+K R +L + N+Y N+ + G Sbjct: 167 RNTISFEVQTDKKSVTAQELDIKARNFLINKKNLY-EFNSSP-------------YETGY 212 Query: 190 VLFHLNNEKSFSYDLF-YTGDGLPVS-FLKIYEDNKIIESEKFHLDV 234 + F NN +F YD+ GD S +L +Y DNK ++S+ ++V Sbjct: 213 IKFIENNGNTFWYDMMPAPGDKFDQSKYLMMYNDNKTVDSKSVKIEV 259
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013420	BACTRLTOXIN	123	2e-36	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 123 bits (310), Expect = 2e-36 Identities = 64/231 (27%), Positives = 111/231 (48%), Gaps = 36/231 (15%) Query: 28 NLRNYYGSYPIEDHQSINPENNHLSHQLVFSMDNST------VTAEFKNVDDVKKFKNHA 81 N++ Y + + + + L+H L++++ + V E N D KK+K+ Sbjct: 50 NMKYLYDDHYVS-ATKVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEV 108 Query: 82 VDVYGLSYSGYCLKNKY------------IYGGVTLA-GDYLEKSRRIPINLWVNGEHQT 128 VDVYG +Y C + +YGG+T G++ + + + V + Sbjct: 109 VDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENKRN 168 Query: 129 ISTDKVSTNKKLVTAQEIDTKLRRYLQEEYNIYGFNDTNKGRNYGNKSKFSSGFNAGKIL 188 + +V T+KK VTAQE+D K R +L + N+Y FN SS + G I Sbjct: 169 TISFEVQTDKKSVTAQELDIKARNFLINKKNLYEFN--------------SSPYETGYIK 214 Query: 189 FHLNDGSSFSYDLFDT-GTGQAES-FLKIYNDNKTVETEKFHLDVEISYKD 237 F N+G++F YD+ G +S +L +YNDNKTV+++ ++V ++ K+ Sbjct: 215 FIENNGNTFWYDMMPAPGDKFDQSKYLMMYNDNKTVDSKSVKIEVHLTTKN 265
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013430	BACTRLTOXIN	170	1e-54	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 170 bits (433), Expect = 1e-54 Identities = 91/262 (34%), Positives = 136/262 (51%), Gaps = 20/262 (7%) Query: 5 LLLILNLIAICSVNNAYANEE-DPKIESLCKKSSVDPIALHNINDDYINNRFTTVKSIVS 63 ++LI LI + S N A + DP + L K S + N+ Y ++ + K V Sbjct: 10 VILIFALILVISTPNVLAESQPDPMPDDLHKSSEFTG-TMGNMKYLYDDHYVSATK--VK 66 Query: 64 TTEKFLDFDLLFKSINWLDGISAEFKDLKVEFSSSAISKEFLGKTVDIYGVYYKAHCH-- 121 + +KFL DL++ D + +K E + ++K++ + VD+YG Y +C+ Sbjct: 67 SVDKFLAHDLIYNI---SDKKLKNYDKVKTELLNEDLAKKYKDEVVDVYGSNYYVNCYFS 123 Query: 122 -----GEHQVDTACTYGGVTPHENNKLSEP--KNIGVAVYKDNVNVNTFIVTTDKKKVTA 174 G+ C YGG+T HE N +N+ V VY++ N +F V TDKK VTA Sbjct: 124 SKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYENKRNTISFEVQTDKKSVTA 183 Query: 175 QELDIKVRTKLNNAYKLYDRMTSDVQKGYIKFHSHSEHKESFYYDLFYIKGNLPDQ--YL 232 QELDIK R L N LY+ +S + GYIKF ++ +F+YD+ G+ DQ YL Sbjct: 184 QELDIKARNFLINKKNLYEFNSSPYETGYIKFIENNG--NTFWYDMMPAPGDKFDQSKYL 241 Query: 233 QIYNDNKTIDSSDYHIDVYLFT 254 +YNDNKT+DS I+V+L T Sbjct: 242 MMYNDNKTVDSKSVKIEVHLTT 263

SAI8T7_1013610

SAI8T7_1013680

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1013610	3	9	-2.756393	Cmp-binding-factor=1
SAI8T7_1013620	2	10	-3.101219	Putative uncharacterized protein
SAI8T7_1013630	-1	13	-2.197925	Probable phosphoesterase
SAI8T7_1013640	-2	11	-1.496601	Hypothetical protein
SAI8T7_1013650	-2	9	-0.840633	UPF0754 membrane protein SaurJH1_1933
SAI8T7_1013660	-2	9	-0.314845	Putative uncharacterized protein
SAI8T7_1013670	-2	9	-0.334651	Two-component response regulator homolog
SAI8T7_1013680	-2	9	0.667096	Sensor histidine kinase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013610	SSPANPROTEIN	29	0.034	Salmonella invasion protein InvJ signature.
>SSPANPROTEIN#Salmonella invasion protein InvJ signature. Length = 336 Score = 28.6 bits (63), Expect = 0.034 Identities = 12/31 (38%), Positives = 19/31 (61%) Query: 128 PAASSHHHNFASGLSYHVLTMLRIAKSICDI 158 PA S HH+ SGL ++ + LRIA+ + + Sbjct: 72 PAKSEHHNGNVSGLHHNGKSELRIAEKLLKV 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013620	cloacin	37	5e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.6 bits (84), Expect = 5e-04 Identities = 40/175 (22%), Positives = 68/175 (38%), Gaps = 36/175 (20%) Query: 145 KQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDR-YEKARAHKQSLERDIGLRNERLAQ 203 KQ++ + QEW + T P + +R YE+ARA D+ ER A+ Sbjct: 297 KQRQDEENRRQQEWDA----------THPVEAAERNYERARAELNQANEDVARNQERQAK 346 Query: 204 LKEEATQLEPVKQSDIDAF-ISLNQQENEIKNKEFELTAIE-------------KDIANK 249 A Q+ ++S++DA +L EI K+F A + Sbjct: 347 ----AVQVYNSRKSELDAANKTLADAIAEI--KQFNRFAHDPMAGGHRMWQMAGLKAQRA 400 Query: 250 QRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLERSLEENK 304 Q D + QA + + ++A S E K K+++ + E +L + K Sbjct: 401 QTDVNNKQA--AFDAAAKEKSDADAALSSAMESRKKKEDKK---RSAENNLNDEK 450
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013670	HTHFIS	80	8e-20	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 80.3 bits (198), Expect = 8e-20 Identities = 36/143 (25%), Positives = 61/143 (42%), Gaps = 7/143 (4%) Query: 3 KVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLKEHEHPDIVLLDLVMPGMN 62 +++ DD +R L L + +V ++ T ++ D+V+ D+VMP N Sbjct: 5 TILVADDDAAIRTVLNQAL-SRAGYDVRI-TSNAATLWRWI-AAGDGDLVVTDVVMPDEN 61 Query: 63 GIEITEYIKAHYPEIKVLVLTSYVDDEHVISAINKGADGYEMKDVEPQQLIETIRRVMNG 122 ++ IK P++ VLV+++ I A KGA Y K + +LI I R + Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121 Query: 123 EKMIHPK----AQDVFETVSQKP 141 K K +QD V + Sbjct: 122 PKRRPSKLEDDSQDGMPLVGRSA 144
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1013680	PF06580	40	8e-06	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 40.2 bits (94), Expect = 8e-06 Identities = 21/111 (18%), Positives = 45/111 (40%), Gaps = 16/111 (14%) Query: 278 IDLSNEIEENIYRA------LQECINNVKKHA-----DTNKMDLTLKQMNDILYIDVIDY 326 + N+I I +Q + N KH K+ L + N + ++V + Sbjct: 240 LQFENQINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENT 299 Query: 327 GQGFEIDNVQIASSHGINNIKQRVKLLRGK---VTFHSQPTKGTQIQFTIP 374 G + N + ++ G+ N+++R+++L G + + K + IP Sbjct: 300 GSL-ALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM-VLIP 348

SAI8T7_1015130

SAI8T7_1015250

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1015130	-1	9	-0.630967	3-isopropylmalate dehydratase small subunit
SAI8T7_1015140	-1	8	-0.146910	L-threonine dehydratase biosynthetic IlvA
SAI8T7_1015180	-1	11	0.267230	**Similar to RNA binding protein, contains S1
SAI8T7_1015190	-3	15	-0.127266	RNA polymerase sigma factor
SAI8T7_1015200	-3	16	-0.663052	Putative Anti-sigma B factor
SAI8T7_1015210	-2	18	-0.354410	Putative RsbU
SAI8T7_1015220	-1	18	0.386689	Alanine racemase
SAI8T7_1015230	0	17	-0.369311	Putative uncharacterized protein
SAI8T7_1015240	-1	13	-0.867402	Putative uncharacterized protein SA1877
SAI8T7_1015250	-1	13	-0.733336	Putative uncharacterized protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015130	NEISSPPORIN	28	0.016	Neisseria sp. porin signature.
>NEISSPPORIN#Neisseria sp. porin signature. Length = 348 Score = 28.4 bits (63), Expect = 0.016 Identities = 18/67 (26%), Positives = 24/67 (35%), Gaps = 4/67 (5%) Query: 40 GFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFH 99 A E RYL D+P+F + G+ DN G H ++ GF Sbjct: 137 EISGMAQREHRYLSVRYDSPEF----AGFSGSVQYAPKDNSGSNGESYHVGLNYQNSGFF 192 Query: 100 IIIAGSF 106 AG F Sbjct: 193 AQYAGLF 199
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015190	FIMREGULATRY	27	0.027	Escherichia coli: P pili regulatory PapB protein si...
>FIMREGULATRY#Escherichia coli: P pili regulatory PapB protein signature. Length = 104 Score = 26.8 bits (59), Expect = 0.027 Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 4/58 (6%) Query: 135 ILEKILPILSDREREIIQCTFIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK 192 +L I I SDR ++ + G S+KE E+ ++ + S T + +L Sbjct: 35 LLIGISSIHSDRVILAMKDYLVGGHSRKEVCEKYQMNNGYFS----TTLGRLIRLNAL 88
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015200	PF06580	35	7e-05	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 35.2 bits (81), Expect = 7e-05 Identities = 9/48 (18%), Positives = 17/48 (35%) Query: 61 VTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSK 108 V N +KH + G I + + + + + G T +S Sbjct: 264 VENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKEST 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015220	ALARACEMASE	328	e-113	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 328 bits (843), Expect = e-113 Identities = 110/366 (30%), Positives = 173/366 (47%), Gaps = 17/366 (4%) Query: 15 RSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVAT 74 R ++DL A+ N + + V +VVKANAYG G ++ + FA+ Sbjct: 3 RPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDG--FALLN 60 Query: 75 LDEAIELRMHGITAKILVL-GVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKK 133 L+EAI LR G IL+L G A+D++ QHR+ V W +A++N + Sbjct: 61 LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCV-HSNWQLKALQNA--RLKAP 117 Query: 134 LWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQLVFEGVFTHFACADEPGDMTTEQYQR 193 L +++K+++GM RLG + + V + ++ + + +HFA A+ P D + R Sbjct: 118 LDIYLKVNSGMNRLGFQ-PDRVLTVWQQLRAMANVGEMTLMSHFAEAEHP-DGISGAMAR 175 Query: 194 FKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSV 253 + NSA +L + +RPGI LYG PS + L+P + Sbjct: 176 IEQAAEG--LECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVM 233 Query: 254 QLIANVVQTKTLQAGESVSYGATYTATDPTTIALLPIGYADGYLR-IMQGSFVNVNGHQC 312 L + ++ +TL+AGE V YG YTA D I ++ GYADGY R G+ V V+G + Sbjct: 234 TLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRT 293 Query: 313 EVIGRVCMDQTIVKVPD--QVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSR 370 +G V MD V + Q G V L ++ VA T+ YE++C L+ Sbjct: 294 MTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEI----KIDDVAAAAGTVGYELMCALAL 349 Query: 371 RLPRIY 376 R+P + Sbjct: 350 RVPVVT 355
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1015250	SECFTRNLCASE	27	0.046	Bacterial translocase SecF protein signature.
>SECFTRNLCASE#Bacterial translocase SecF protein signature. Length = 333 Score = 26.7 bits (59), Expect = 0.046 Identities = 10/18 (55%), Positives = 13/18 (72%) Query: 28 LSVAVYVIFYFIWLRFEW 45 L A VI ++IW+RFEW Sbjct: 159 LLAATVVIMFYIWVRFEW 176

SAI8T7_1016170

SAI8T7_1016220

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1016170	-2	8	-1.734840	Iron complex transport system substrate-binding
SAI8T7_1016180	-2	8	-1.495369	Putative uncharacterized protein
SAI8T7_1016190	-2	7	-0.597128	Putative uncharacterized protein
SAI8T7_1016200	-2	8	-0.383817	Transporter
SAI8T7_1016210	-2	8	0.522783	Putative uncharacterized protein
SAI8T7_1016220	-1	9	1.058634	Alkaline shock protein 23

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016170	FERRIBNDNGPP	96	6e-25	Ferrichrome-binding periplasmic protein signature.
>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature. Length = 296 Score = 95.8 bits (238), Expect = 6e-25 Identities = 64/257 (24%), Positives = 107/257 (41%), Gaps = 24/257 (9%) Query: 63 DAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIK--PVREKIGDYTSVGTRKQPNLE 120 D RIV LE+ + L AL + P G+AD + + P+ + + D VG R +PNLE Sbjct: 34 DPNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPPLPDSVID---VGLRTEPNLE 90 Query: 121 EISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNI--NSFKTIAKALNKEK 178 ++++KP ++ S+ + + L +IAP DG + S +A LN + Sbjct: 91 LLTEMKPSFMVW-SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQS 149 Query: 179 EGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVV---AKAGLLAHPNYSYVGQFLNELGFK 235 E LA+++ I K R + L + L+ PN S + L+E G Sbjct: 150 AAETHLAQYEDFIRSMKPRF-VKRGARPLLLTTLIDPRHMLVFGPN-SLFQEILDEYGIP 207 Query: 236 NALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKKLQEDATWKK 295 NA +G + + + + LA ++ DH +S + L W+ Sbjct: 208 NAW-----QGETNFWG--STAVSIDRLAAYKDVDVLCF-DHD--NSKDMDALMATPLWQA 257 Query: 296 LNAVKNNRVDIVDRDVW 312 + V+ R V VW Sbjct: 258 MPFVRAGRFQRVP-AVW 273
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016180	ALARACEMASE	39	1e-05	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 39.4 bits (92), Expect = 1e-05 Identities = 59/325 (18%), Positives = 119/325 (36%), Gaps = 33/325 (10%) Query: 4 VNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVESLKALG-INHVAESRLDNI 62 ++++ +K N +++ + + + V+K A I A+G + A L+ Sbjct: 7 ASLDLQALKQNLSIVRQA--ATHARVWSVVKANAYGHGIERIWSAIGATDGFALLNLEEA 64 Query: 63 ISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIHQINEVAEV-LGKKHKILLMV 121 I++ ++ +L D+ + T + + Q+ + L I L V Sbjct: 65 ITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKV 124 Query: 122 DWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCFKSDAPSDDDIFMINRFVSAVE 181 + R G VL +++ + N+ + L +F ++ P D + R A E Sbjct: 125 NSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAE--AEHP-DGISGAMARIEQAAE 181 Query: 182 REIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGETLFRGVDTTTNQAIAML-YQDAIT 240 + R + + + P+ ++ +R G L+ + + IA + +T Sbjct: 182 -GLECRRSLSNSAATLWHPEAHFD------WVRPGIILYGASPSGQWRDIANTGLRPVMT 234 Query: 241 LEAEILEIK-----PRVN-----TQTHESFLQAIVDIGYLD---TKVDNISPM---DQHI 284 L +EI+ ++ RV T E + IV GY D +P+ Sbjct: 235 LSSEIIGVQTLKAGERVGYGGRYTARDEQRI-GIVAAGYADGYPRHAPTGTPVLVDGVRT 293 Query: 285 NILGA-SSDHLMLDLNGQGHYQVGD 308 +G S D L +DL +G Sbjct: 294 MTVGTVSMDMLAVDLTPCPQAGIGT 318
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016190	PF04183	259	2e-81	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 259 bits (663), Expect = 2e-81 Identities = 92/456 (20%), Positives = 176/456 (38%), Gaps = 56/456 (12%) Query: 121 EGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGND--QFIIDE 178 GHP K + E + YAPE+ L + ++++H++ + D Q + Sbjct: 134 SGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAA 193 Query: 179 IIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT-PFTILS 237 + P+ + + + GL ++ + VHPWQ+ I F A A+ ++ F Sbjct: 194 MDPQEFARFSQVWQENGL-DHNWLPLPVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQW 252 Query: 238 KATLSFRTMSLIDKP--YHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQN-------- 287 A S RT++ + +KLP+ TS R + GP S LQ Sbjct: 253 LAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATL 312 Query: 288 ------MLNQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPE--IDGKGATVVSASLV 339 +L + V+ E + A L I R+ P + + V+ A+L+ Sbjct: 313 VQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLM 372 Query: 340 NKNPIDQKVIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNT 399 + +Q + +Y++ G+ E+ ++ + + ++ PL + YG+AL AH QN Sbjct: 373 ECDENNQPLA-GAYID--RSGLDAET---WLTQLFRVVVVPLYHLLCRYGVALIAHGQNI 426 Query: 400 VVNLGPHFDIQFLVRDLGGS-RI------DLETLQHRVSDI--KITNDSLIADSIDAVIA 450 + + + L++D G R+ ++++L V D+ +++ D LI D Sbjct: 427 TLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFV 486 Query: 451 KFQHAVIQNQMAELIHHFNQYDCVEETELFNIVQQVVA--HAINPTLPHANELKDILFGP 508 I V E + ++ V++ +P + L LF P Sbjct: 487 TV---------LRFISPLMVRLGVPERRFYQLLAAVLSDYMKKHPQMSERFALFS-LFRP 536 Query: 509 TITVKALLNMRM-----ENKVKQYLNI--ELDNPIK 537 I L +++ + + N +L NP+ Sbjct: 537 QIIRVVLNPVKLTWPDLDGGSRMLPNYLEDLQNPLW 572
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016200	TCRTETA	42	3e-06	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 41.7 bits (98), Expect = 3e-06 Identities = 53/340 (15%), Positives = 106/340 (31%), Gaps = 26/340 (7%) Query: 6 FSSSFLLFLGNWIGQIGLNWFVLTTYHN--------AVYLGIVNFCRLVPILLLSVWAGA 57 S+ L +G IGL VL + GI+ + + GA Sbjct: 11 LSTVALDAVG-----IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGA 65 Query: 58 IADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT-LRGILSAVETPLRQ 116 ++D++ + R + S A+ Y+ A + ++Y + ++ + Sbjct: 66 LSDRFGR----RPVLLVSLAGAAV----DYAIMATAPFLWVLYIGRIVAGITGATGAVAG 117 Query: 117 AILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAPTTFLAQA--ICYFIAA 174 A + D++D + F S GP + G++ F A A F+ Sbjct: 118 AYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTG 177 Query: 175 LLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSLLIMATGFSYTTLLPVL 234 LP K + P PL + + + ++ G L + Sbjct: 178 CFLLPESHKGERRPLRREALNPLASFRWARGMTVVA-ALMAVFFIMQLVGQVPAALWVIF 236 Query: 235 TNKVFPGKSEIFGIAMTMCAIGGIIATLVL-PKVLKYIGMVNMYYLSSLLFGIALLGVVF 293 F + GI++ I +A ++ V +G L + G + + F Sbjct: 237 GEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAF 296 Query: 294 HNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKV 333 M I ++ + V + +G++ Sbjct: 297 ATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQL 336 Score = 30.6 bits (69), Expect = 0.012 Identities = 37/180 (20%), Positives = 71/180 (39%), Gaps = 21/180 (11%) Query: 10 FLLFLGNWIGQIGLNWFVLTTYH----NAVYLGI-VNFCRLVPILLLSVWAGAIADKYDK 64 + F+ +GQ+ +V+ +A +GI + ++ L ++ G +A + + Sbjct: 217 AVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE 276 Query: 65 GRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYATLRGILSAVETPLRQAILPDLSD 124 R L + + + +L T + A PI V++ + P QA+L D Sbjct: 277 RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLA-------SGGIGMPALQAMLSRQVD 329 Query: 125 KISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAPT----TFLAQAICYFIAALLCLPL 180 + Q + + ++ +GP + I A T ++A A Y LLCLP Sbjct: 330 EERQGQLQGSLAALTSLTSIVGPLLFTAIYA-ASITTWNGWAWIAGAALY----LLCLPA 384
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016210	PF04183	270	3e-84	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 270 bits (691), Expect = 3e-84 Identities = 93/475 (19%), Positives = 181/475 (38%), Gaps = 45/475 (9%) Query: 197 SEQAVIEGHPLHPGAKLRKGLNALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDT 256 Q ++ GHP K R+G Y+ E+ +L + + + M D + Sbjct: 128 RLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKRE---HMIWRCDNEM 184 Query: 257 TVHQLF-----PDLIKQLENEFTPNFNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIE 311 +HQL P + + N +++ + VHPWQ + +D+ A+ + ++ Sbjct: 185 DIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEGRMVS 244 Query: 312 AKHTLD-YYAGLSFRTLVPKYPAMSPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILND 370 D + A S RTL IKL ++ T R + + GPL +R L Sbjct: 245 LGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWLQQ 304 Query: 371 ILEKDVIFKSYASTIIDEVAGIHFYNEQDEVDYQTER--SEQLGTLFRKNIYQMIPQEVT 428 + D + I+ E A + +E + E LG ++R+N + + + + Sbjct: 305 VFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLKPDES 364 Query: 429 PMIPSSLVATYPFNNESPIVTLIKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTK 488 P++ ++L+ N P+ A + A++W+ + ++ + L+ + Sbjct: 365 PVLMATLMECDE--NNQPLA--------GAYIDRSGLDAETWLTQLFRVVVVPLYHLLCR 414 Query: 489 YGIALEAHLQNAIATFRKDGLLDTMYIRDFEG-LRIDKAQLNEMGYSTSHFHEKSRILTD 547 YG+AL AH QN I K+G+ + ++DF+G +R+ K + EM S E + + Sbjct: 415 YGVALIAHGQN-ITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMD---SLPQEVRDVTSR 470 Query: 548 SKTSVFNKAFYSTVQNHLGELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKS 607 + + I + ER + ++ VL + + H Sbjct: 471 LSADYLIHDLQTGHFVTVLRFISPLMVRLGVP--ERRFYQLLAAVLSDYMKK-----HPQ 523 Query: 608 NQVNENRINEIKDTMFAPFIDYKCVTTMRLE----DEAHHY--TYIK-VNNPLYR 655 + +F P I + ++L D Y++ + NPL+ Sbjct: 524 MSERFALFS-----LFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEDLQNPLWL 573
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1016220	TCRTETOQM	29	0.012	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 28.7 bits (64), Expect = 0.012 Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 5/43 (11%) Query: 99 VDLKVILEYGE-----SAPKIFRKVTELVKEQVKYITGLDVVE 136 D K+ +YG S P FR + +V EQV G +++E Sbjct: 495 TDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE 537

SAI8T7_1017370

SAI8T7_1017480

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1017370	0	10	-1.582581	Putative uncharacterized protein
SAI8T7_1017380	1	8	-1.288830	Similar to esterase
SAI8T7_1017390	0	8	-1.501127	Putative uncharacterized protein
SAI8T7_1017400	-1	8	-1.549153	SA2142 protein
SAI8T7_1017410	-2	12	-1.367301	Putative uncharacterized protein SA2143
SAI8T7_1017420	-2	13	-1.810388	Similar to transcriptional regulator
SAI8T7_1017430	-2	14	-1.189832	TcaB protein
SAI8T7_1017440	-2	11	-1.467736	Putative Membrane-associated protein TcaA
SAI8T7_1017450	-1	10	-1.454390	Putative uncharacterized protein
SAI8T7_1017460	-2	11	-0.213017	Putative hemin import ATP-binding protein HrtA
SAI8T7_1017470	0	12	-0.718543	Putative hemin transport system permease protein
SAI8T7_1017480	-1	11	-0.395938	Response regulator receiver domain protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017370	PF06438	26	0.050	Heme acquisition protein HasAp
>PF06438#Heme acquisition protein HasAp Length = 205 Score = 26.5 bits (58), Expect = 0.050 Identities = 12/28 (42%), Positives = 17/28 (60%), Gaps = 2/28 (7%) Query: 68 NLAYTLFTLEEHTTY--LSELSLGDVFT 93 +L YTLF+ HT + L ++LGD T Sbjct: 72 DLHYTLFSNPSHTLWGKLDSIALGDTLT 99
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017400	TCRTETB	158	2e-44	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 158 bits (402), Expect = 2e-44 Identities = 92/415 (22%), Positives = 187/415 (45%), Gaps = 16/415 (3%) Query: 140 KILAALLFGMFIAILNQTLLNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLF 199 +IL L F ++LN+ +LNV+LP I +FN ++ W+ T FML I + L Sbjct: 14 QILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLS 73 Query: 200 NKYSYRKLFLVALVLFTIGSLICAISMN-FPIMMVGRVLQAIGAGVLMPLGSIVIITIYP 258 ++ ++L L +++ GS+I + + F ++++ R +Q GA L +V+ P Sbjct: 74 DQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIP 133 Query: 259 PEKRGAAMGTMGIAMILAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLY 318 E RG A G +G + + +GP + G I HW+ + +I II + K Sbjct: 134 KENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIP-MITIITVPFLMKLLKKE 192 Query: 319 QYTTNPKADIPGIIFSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELR 378 DI GII ++G + + + ++ ++FV + Sbjct: 193 VRIKGH-FDIKGIILMSVGIVFFMLF---TTSYSIS------FLIVSVLSFLIFVKHIRK 242 Query: 379 MKSPMLNLEVLKFPTFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSG-LLLLPG 437 + P ++ + K F + + ++ ++ G + ++P ++++ S + G +++ PG Sbjct: 243 VTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPG 302 Query: 438 SLIMGLLGPFAGKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTP-YMTIMGIYVLRSF 496 ++ + + G G L+D G + G+ ++ + + L T +MTI+ ++VL Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVL--G 360 Query: 497 GMAFIMMPMVTAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQH 551 G++F + T ++L + A G + LN L+ G AI+ +++ Sbjct: 361 GLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQ 415
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017410	RTXTOXIND	59	1e-12	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 59.1 bits (143), Expect = 1e-12 Identities = 26/133 (19%), Positives = 45/133 (33%), Gaps = 13/133 (9%) Query: 87 MDLKMPQKGTIAKLD-GMEGSMVQAGNPIAYAYNLDD-LYVTANIDEKDIKDVEVGKDVD 144 ++ P + +L EG +V + DD L VTA + KDI + VG++ Sbjct: 328 SVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAI 387 Query: 145 VTIDGQKAS----IKGKVDSIGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSK 200 + ++ + + GKV +I G V I + Sbjct: 388 IKVEAFPYTRYGYLVGKVKNINLDAI-------EDQRLGLVFNVIISIEENCLSTGNKNI 440 Query: 201 QVVPGMNAEVKIH 213 + GM +I Sbjct: 441 PLSSGMAVTAEIK 453 Score = 32.5 bits (74), Expect = 0.001 Identities = 17/77 (22%), Positives = 35/77 (45%), Gaps = 2/77 (2%) Query: 9 VITVVVLLAIGIAGFYFWNKTTSYVTTDNAKV--NGDQIKIASPASGQIKSLNVKQGDKL 66 ++ ++ + IA V T N K+ +G +I + +K + VK+G+ + Sbjct: 59 LVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESV 118 Query: 67 DKGDKVATVTVQGQDGE 83 KGD + +T G + + Sbjct: 119 RKGDVLLKLTALGAEAD 135
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017420	HTHTETR	45	4e-08	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 44.6 bits (105), Expect = 4e-08 Identities = 13/69 (18%), Positives = 24/69 (34%) Query: 2 KRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDYYKDKFDLLDTINSKHKE 61 ++ + I + + L ++ S I + R Y ++KDK DL I + Sbjct: 9 AQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSES 68 Query: 62 KFQFLLSAL 70 L Sbjct: 69 NIGELELEY 77
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017430	TCRTETA	64	2e-13	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 64.5 bits (157), Expect = 2e-13 Identities = 68/386 (17%), Positives = 141/386 (36%), Gaps = 16/386 (4%) Query: 15 IIILGSLTAIGALSIDMFLPGLPDIRHDF---QTTTSNAQLTLSMFMIGLAFGNLFAGPI 71 +I++ S A+ A+ I + +P LP + D T++ + L+++ + G + Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66 Query: 72 SDSTGRRKPLIIAMIIFTLASLGIVFVHNIWLMVALRFLQGVTGGAAAVISRAIASDMYS 131 SD GRR L++++ + + +W++ R + G+TG AV IA D+ Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA-DITD 125 Query: 132 GNELTKFMALLMLVNGIAPVVAPTIGGIILNYSVWRMVFVILTIFGFVMVIGSLLKVPES 191 G+E + + G V P +GG++ +S F + + +PES Sbjct: 126 GDERARHFGFMSACFGFGMVAGPVLGGLMGGFSP-HAPFFAAAALNGLNFLTGCFLLPES 184 Query: 192 LTVTNRESSSGLKTMFKNFKILLKTPRFVLPMLIQGMTFVILFTYISASPFII--QKIYG 249 R +F+ + V ++ + L + A+ ++I + + Sbjct: 185 HKGERRPLRREALNPLASFR-WARGMTVVAALMAVFFI-MQLVGQVPAALWVIFGEDRFH 242 Query: 250 MTAIQFSWMFAGIGITLIISSQLTGYLVDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWN 309 A A GI ++ + + ++ R M+ +I I+L Sbjct: 243 WDATTIGISLAAFGILHSLAQ---AMITGPVAARLGERRALMLGMIADGTGYILLAFATR 299 Query: 310 FWILAIGFIILIAPVTGVATIGFTIAMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKG 369 W+ ++L + G+ + ++ +G L + L + PL+ Sbjct: 300 GWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSL-TSIVGPLLFTAI 358 Query: 370 EDNPIPY---IIIIIATAVILIILQI 392 I I A+ L+ L Sbjct: 359 YAASITTWNGWAWIAGAALYLLCLPA 384
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017460	PF05272	29	0.014	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 29.3 bits (65), Expect = 0.014 Identities = 11/21 (52%), Positives = 14/21 (66%) Query: 35 VILNGASGSGKTTLLTILGGL 55 V+L G G GK+TL+ L GL Sbjct: 599 VVLEGTGGIGKSTLINTLVGL 619
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017480	HTHFIS	79	3e-19	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 79.5 bits (196), Expect = 3e-19 Identities = 31/117 (26%), Positives = 51/117 (43%), Gaps = 1/117 (0%) Query: 14 LVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQRVDIAVVDIMMDGMDGFQLC 73 LV DDD I + L D + + + D+ V D++M + F L Sbjct: 7 LVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLL 66 Query: 74 NTLKNDY-DIPVIMLTARDALSDKERAFISGTDDYVTKPFEVKELIFRIRAVLRRYN 129 +K D+PV++++A++ +A G DY+ KPF++ ELI I L Sbjct: 67 PRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

SAI8T7_1017880

SAI8T7_1017970

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1017880	-2	14	-0.547367	Similar to multidrug resistance protein homolog
SAI8T7_1017890	0	18	-0.979735	2,3-bisphosphoglycerate-dependent
SAI8T7_1017900	0	17	-1.329491	Similar to cation efflux family protein
SAI8T7_1017910	1	17	-1.417049	Immunoglobulin-binding protein sbi
SAI8T7_1017920	0	16	-1.818509	Gamma-hemolysin component A, HlgA
SAI8T7_1017930	-2	16	-1.547727	Gamma-hemolysin component C
SAI8T7_1017940	-2	15	-1.321672	Gamma-hemolysin component B
SAI8T7_1017950	-2	18	-1.318028	Putative uncharacterized protein
SAI8T7_1017960	-2	16	-1.834492	6-carboxyhexanoate--CoA ligase
SAI8T7_1017970	-2	14	-2.052065	Similar to 8-amino-7-oxononanoate synthase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017880	TCRTETB	129	3e-35	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 129 bits (327), Expect = 3e-35 Identities = 91/398 (22%), Positives = 177/398 (44%), Gaps = 14/398 (3%) Query: 18 FFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFL 77 FF +LNE +L +LP I DF W+ TAF+LT I + + + +++ L Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83 Query: 78 VGISIFFLGTLLGGLS-PHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGI 136 GI I G+++G + F+ L++AR IQ GA L+M + RGK G+ Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143 Query: 137 FGLVIGLAPAIGPTLSGYLVEYLNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDF 196 G ++ + +GP + G + Y++W L + P+ + + + IK D Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHYIHWSYLLLI--PMITIITVPFLMKLLKKEVRIKGHFDI 201 Query: 197 ISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAV 256 +I +G + T+S S F +I V+ +FV +++ P ++ + Sbjct: 202 KGIILMSVGIVFFMLFTTSYS-ISF--------LIVSVLSFLIFVKHIRKVTDPFVDPGL 252 Query: 257 FKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSG-LTLLPGAIVMAFMSMT 315 KN F +G++ G+ ++ G +++P ++++ S + G + + PG + + Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312 Query: 316 SGALYEKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMT 375 G L ++ GP + +G+ + ++ + E TS M + + G++ + T Sbjct: 313 GGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSFTKTVIST 371 Query: 376 HTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILS 413 + LK + G S+ N ++ G A + +LS Sbjct: 372 IVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017920	BICOMPNTOXIN	427	e-154	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 427 bits (1100), Expect = e-154 Identities = 213/312 (68%), Positives = 247/312 (79%), Gaps = 8/312 (2%) Query: 13 MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGA--EIIKRTQDITSKRLAITQ 70 M+KNKILT TL+V L+APLANP +E +KA N EDIG+G+ EIIKRT+D TS + +TQ Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 71 NIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNV 130 NIQFDFVKDKKYNKDAL++KMQGFISSRTTY + KK ++K M WPFQYNI LKT D V Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYV 120 Query: 131 DLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQ 190 LINYLPKNKI+S +VSQ LGYNIGGNFQSAPS+GG+GSFNYSK+ISY Q+NYV+EVE Q Sbjct: 121 SLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQ 180 Query: 191 NSKGVKWGVKANSFVTPNGQVSAYDQYLF-AQDPTGPAARDYFVPDNQLPPLIQSGFNPS 249 NSK V WGVKANSF T +GQ SA+D LF P RDYFVPD++LPPL+QSGFNPS Sbjct: 181 NSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPS 240 Query: 250 FITTLSHERGKGDKSEFEITYGRNMDATYA-----YVTRHRLAVDRKHDAFKNRNVTVKY 304 FI T+SHE+G D SEFEITYGRNMD T+A + L R H+AF NRN TVKY Sbjct: 241 FIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKY 300 Query: 305 EVNWKTHEVKIK 316 EVNWKTHE+K+K Sbjct: 301 EVNWKTHEIKVK 312
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017930	BICOMPNTOXIN	467	e-169	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 467 bits (1203), Expect = e-169 Identities = 313/315 (99%), Positives = 313/315 (99%) Query: 1 MLKNKILATTLSVSLLALLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 MLKNKIL TTLSVSLLA LANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYV 120 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYV Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYV 120 Query: 121 SLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQ 180 SLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQ Sbjct: 121 SLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQ 180 Query: 181 NSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPS 240 NSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPS Sbjct: 181 NSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPS 240 Query: 241 FIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKY 300 FIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKY Sbjct: 241 FIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKY 300 Query: 301 EVNWKTHEIKVKGQN 315 EVNWKTHEIKVKGQN Sbjct: 301 EVNWKTHEIKVKGQN 315
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017940	BICOMPNTOXIN	383	e-136	Staphylococcal bi-component toxin signature.
>BICOMPNTOXIN#Staphylococcal bi-component toxin signature. Length = 315 Score = 383 bits (985), Expect = e-136 Identities = 87/322 (27%), Positives = 160/322 (49%), Gaps = 18/322 (5%) Query: 1 MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTLYKTTATADSDKFKISQ 60 M NK++ ++++ S+ L + + + K T S+K+ ++Q Sbjct: 1 MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQ 60 Query: 61 ILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSK-LYWGAKYNVSISSQSNDS 119 + F+F+KDK Y+KD L+LK G I+S N + K + W +YN+ + + ++ Sbjct: 61 NIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKT-NDKY 119 Query: 120 VNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNGNTAFSETINYKQESYRTTL 179 V++++Y PKN+ E V TLGY GG+ + L G NG+ +S++I+Y Q++Y + + Sbjct: 120 VSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGG--NGSFNYSKSISYTQQNYVSEV 177 Query: 180 SRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQM 239 + N K+V WGV+A+ + ++LF+ + S F+ ++ Sbjct: 178 EQQ-NSKSVLWGVKANSFATESGQ-------KSAFDSDLFVGYKPHSKDPRDYFVPDSEL 229 Query: 240 PLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMDL-----YQIRWNGFYWAGANYKN 294 P L +S FNP F++ +SH + + S+ +TY R MD+ + Y G N Sbjct: 230 PPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHN 289 Query: 295 -FKTRTFKSTYEIDWENHKVKL 315 F R + YE++W+ H++K+ Sbjct: 290 AFVNRNYTVKYEVNWKTHEIKV 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1017970	CLENTEROTOXN	28	0.044	Clostridium enterotoxin signature.
>CLENTEROTOXN#Clostridium enterotoxin signature. Length = 319 Score = 28.1 bits (62), Expect = 0.044 Identities = 8/47 (17%), Positives = 15/47 (31%), Gaps = 3/47 (6%) Query: 172 GGVILSSND---VKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN 215 IL+ N+ L I + + FI+ ++E Sbjct: 59 SSQILNPNETGTFSQSLTKSKEVSINVNFSVGFTSEFIQASVEYGFG 105

SAI8T7_1019110

SAI8T7_1019180

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1019110	1	11	0.839411	Acetyltransferase, GNAT family
SAI8T7_1019120	1	12	1.496157	Probable transglycosylase isaA
SAI8T7_1019130	-1	11	0.966870	Putative regulatory protein
SAI8T7_1019140	-1	10	0.532243	Putative uncharacterized protein
SAI8T7_1019150	-3	11	1.366605	Putative uncharacterized protein
SAI8T7_1019160	-3	11	1.393406	Putative uncharacterized protein
SAI8T7_1019170	-2	13	1.606375	HTH-type transcriptional regulator SAV2578
SAI8T7_1019180	0	14	2.105867	Putative short chain oxidoreductase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019110	SACTRNSFRASE	43	8e-08	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 43.0 bits (101), Expect = 8e-08 Identities = 31/101 (30%), Positives = 46/101 (45%), Gaps = 5/101 (4%) Query: 41 DDQPDLENIEHNYLNSGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKI 100 DD D+ +E G +L N +G I + N + ++ + V K YR + Sbjct: 52 DDDMDVSYVE----EEGKAAFLYYLE-NNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGV 106 Query: 101 GKKLLDKVIMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFR 141 G LL K I KE + G+ L T D ISA +FY+ + F Sbjct: 107 GTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI 147
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019140	HTHTETR	43	1e-07	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 43.5 bits (102), Expect = 1e-07 Identities = 33/200 (16%), Positives = 64/200 (32%), Gaps = 34/200 (17%) Query: 11 KSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIATLNRATFYAHFTDKEDLLDYT 70 + T+Q ++D L++ ++ +S ++ +I A + R Y HF DK DL Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 71 LSV---TILKDLNDNLSISNVINEKVLRNIFISIASYIKDAAKSCELNSEAFCNKAHQRI 127 + I + + + VLR I I + + L F Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIF------HK 116 Query: 128 NNELEDIFAIM-LENSYPEHQRDIIVNS-------------------ASFLAAGISGLAL 167 + ++ + + + D I + A + ISGL Sbjct: 117 CEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLME 176 Query: 168 HWFNTSQ-----ETADVFID 182 +W Q + A ++ Sbjct: 177 NWLFAPQSFDLKKEARDYVA 196
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019160	NUCEPIMERASE	36	2e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 35.5 bits (82), Expect = 2e-04 Identities = 35/138 (25%), Positives = 53/138 (38%), Gaps = 35/138 (25%) Query: 1 MKDILVIGATGKQGNAVVKQLLEDGWYVSAL--------TRNKNNRKLSDIGHPHLSIVE 52 MK LV GA G G V K+LLE G V + K R L + P + Sbjct: 1 MK-YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQAR-LELLAQPGFQFHK 58 Query: 53 GDLSD-----------------NVSLQSAMKGKYGLYSIQ-PIVKDDVSEELRQGMKIIE 94 DL+D + A++ YS++ P D + L + I+E Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVR-----YSLENPHAYADSN--LTGFLNILE 111 Query: 95 IAEQENIQHIVYSTAGGV 112 IQH++Y+++ V Sbjct: 112 GCRHNKIQHLLYASSSSV 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019170	HTHTETR	62	2e-14	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 62.3 bits (151), Expect = 2e-14 Identities = 25/80 (31%), Positives = 44/80 (55%) Query: 1 MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTLYRHFKDKSDLCYYVIQ 60 +++A+E RQ I ++A +LF ++GV + S+ IAK G+ G +Y HFKDKSDL + + Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 61 RDLDIFITHFKQIKDDYHSN 80 + + + + Sbjct: 65 LSESNIGELELEYQAKFPGD 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019180	DHBDHDRGNASE	65	6e-15	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 65.5 bits (159), Expect = 6e-15 Identities = 46/194 (23%), Positives = 74/194 (38%), Gaps = 18/194 (9%) Query: 2 LITGGNKGLGYASAEALKALGYKVYIGSRND---VRGQQASQKLGVHYVQ--LDVTSDYS 56 ITG +G+G A A L + G + N + + + H DV + Sbjct: 12 FITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSAA 71 Query: 57 VKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEVYQTNVFGIVRMMNTFVPL 116 + I + G +DIL+N AG+ + L+ + E + N G+ + Sbjct: 72 IDEITARIEREMGPIDILVNVAGVL-RPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130 Query: 117 LEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSSKSAVTMLTLQYAKGLP--NM 174 + +V V S P T+ + AY SSK+A M T L N+ Sbjct: 131 MMDRRSGSIVTVGSNPAG-----VPRTSMA-----AYASSKAAAVMFTKCLGLELAEYNI 180 Query: 175 QINAADPGATNTDL 188 + N PG+T TD+ Sbjct: 181 RCNIVSPGSTETDM 194

SAI8T7_1019650

SAI8T7_1019720

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1019650	1	15	2.113291	Clumping factor B
SAI8T7_1019660	-1	12	0.127131	HTH-type transcriptional regulator ArcR
SAI8T7_1019670	-3	10	1.107033	Carbamate kinase 2
SAI8T7_1019680	-2	10	0.360113	Arginine/ornithine antiporter
SAI8T7_1019690	-3	10	0.041238	Ornithine carbamoyltransferase, catabolic
SAI8T7_1019700	-1	8	-0.761311	Arginine deiminase
SAI8T7_1019710	-1	10	0.438555	Arginine repressor 1
SAI8T7_1019720	-1	10	0.974584	Zinc metalloproteinase aureolysin

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019650	PF05616	51	2e-08	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 50.9 bits (121), Expect = 2e-08 Identities = 36/125 (28%), Positives = 57/125 (45%), Gaps = 14/125 (11%) Query: 508 NVDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPK-----DPTPG----PPVDPEPSP 558 N+ PVT+R+ N VV G + G++ V+ + D TPG P P P Sbjct: 277 NMGPVTDRN-----GNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEV 331 Query: 559 DPEPEPTPDPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDS 618 P P +P P+ +P P+P+PDPD + D++ +D G+ DS + D + Sbjct: 332 SPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGRHRKE 391 Query: 619 DSDSE 623 + E Sbjct: 392 RKEGE 396 Score = 35.1 bits (80), Expect = 0.001 Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 1/63 (1%) Query: 538 DSAVNPK-DPTPGPPVDPEPSPDPEPEPTPDPEPSPDPEPEPSPDPDPDSDSDSDSGSDS 596 + A NP + PG +PEP PD P+ PD + P P+ PD + + Sbjct: 336 NPANNPAPNENPGTRPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGRHRKERKEG 395 Query: 597 DSG 599 + G Sbjct: 396 EDG 398
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019670	CARBMTKINASE	388	e-138	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 388 bits (999), Expect = e-138 Identities = 137/314 (43%), Positives = 198/314 (63%), Gaps = 5/314 (1%) Query: 1 MKEKIVIALGGNAIQT--KEATAEAQQTAIRRAMQNLKPLFDSPARIVISHGNGPQIGSL 58 M +++VIALGGNA+Q ++ + E +R+ + + + +VI+HGNGPQ+GSL Sbjct: 1 MGKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSL 60 Query: 59 LIQQAKSNSDT-TPAMPLDTCGAMSQGMIGYWLETEINRILTEMNSDRTVGTIVTRVEVD 117 L+ + PA P+D GAMSQG IGY ++ + L + ++ V TI+T+ VD Sbjct: 61 LLHMDAGQATYGIPAQPMDVAGAMSQGWIGYMIQQALKNELRKRGMEKKVVTIITQTIVD 120 Query: 118 KDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYRKVVASPLPQSILEHQLI 177 K+DP F NPTKP+GPFY +E + L +E + KED+GRG+R+VV SP P+ +E + I Sbjct: 121 KNDPAFQNPTKPVGPFYDEETAKRLARE-KGWIVKEDSGRGWRRVVPSPDPKGHVEAETI 179 Query: 178 RTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASEKLATLIEADTLMILTNVEN 237 + L + IVIA GGGG+PVI ++ +GVEAVIDKD A EKLA + AD MILT+V Sbjct: 180 KKLVERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNG 239 Query: 238 VFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKIEAAIRFVESGENKKVIITNLE 297 + + +Q + ++ V L+KY +G F GSM PK+ AAIRF+E G ++ II +LE Sbjct: 240 AALYYGTEKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWG-GERAIIAHLE 298 Query: 298 QAYEALIGNKGTHI 311 +A EAL G GT + Sbjct: 299 KAVEALEGKTGTQV 312
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019700	ARGDEIMINASE	506	0.0	Bacterial arginine deiminase signature.
>ARGDEIMINASE#Bacterial arginine deiminase signature. Length = 409 Score = 506 bits (1305), Expect = 0.0 Identities = 193/409 (47%), Positives = 275/409 (67%), Gaps = 8/409 (1%) Query: 5 PIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIPYLEVAQKEHDHFAQVLREEG 64 PI + SEIG LK VLL RPG+ELENL P + LFDDIPYLEVA++EH+ FA +L+ Sbjct: 7 PINIFSEIGRLKKVLLHRPGEELENLTPFIMKNFLFDDIPYLEVARQEHEVFASILKNNL 66 Query: 65 VEVLYLEKLAAESIENPQ-VRSEFIDDVLAESKKTILGHEEEIKTLFATLSNQELVDKIM 123 VE+ Y+E L +E + + + ++FI + E++ +K F++L+ ++ K++ Sbjct: 67 VEIEYIEDLISEVLVSSVALENKFISQFILEAEIKTDFTINLLKDYFSSLTIDNMISKMI 126 Query: 124 SGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTRDPQASIGHGITINRMFWRARRRE 183 SGV EE+ + L + ++ F +DPMPN+ FTRDP ASIG+G+TIN+MF + R+RE Sbjct: 127 SGVVTEELKNYTSSLDDLVNGANLFIIDPMPNVLFTRDPFASIGNGVTINKMFTKVRQRE 186 Query: 184 SIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGDELVLSKDVLAIGVSERTSAQAIEKL 243 +IF +YI K+HP +K N+PIWL+R ++EGGDELVL+K +L IG+SERT A+++EKL Sbjct: 187 TIFAEYIFKYHPVYK-ENVPIWLNRWEEASLEGGDELVLNKGLLVIGISERTEAKSVEKL 245 Query: 244 ARRIFENPQATFKKVVAIEIPTSRTFMHLDTVFTMIDYDKFTMHSAILKAEGNMNIFIIE 303 A +F+N + +F ++A +IP +R++MHLDTVFT IDY FT ++ + +I+++ Sbjct: 246 AISLFKN-KTSFDTILAFQIPKNRSYMHLDTVFTQIDYSVFTSFTSD---DMYFSIYVLT 301 Query: 304 YDDVNKDIAIK-QSSHLKDTLEDVLGIDDIQFIPTGNGDVIDGAREQWNDGSNTLCIRPG 362 Y+ + I IK + + +KD L LG I I GD+I GAREQWNDG+N L I PG Sbjct: 302 YNPSSSKIHIKKEKARIKDVLSFYLG-RKIDIIKCAGGDLIHGAREQWNDGANVLAIAPG 360 Query: 363 VVVTYDRNYVSNDLLRQKGIKVIEISGSELVRGRGGPRCMSQPLFREDI 411 ++ Y RN+V+N L + GIKV I SEL RGRGGPRCMS PL REDI Sbjct: 361 EIIAYSRNHVTNKLFEENGIKVHRIPSSELSRGRGGPRCMSMPLIREDI 409
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019710	ARGREPRESSOR	83	7e-23	Bacterial arginine repressor signature.
>ARGREPRESSOR#Bacterial arginine repressor signature. Length = 149 Score = 82.6 bits (204), Expect = 7e-23 Identities = 38/147 (25%), Positives = 78/147 (53%), Gaps = 2/147 (1%) Query: 18 MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAKDLKELNIYRVPIDCET 77 M K +R + I+ ++I +++++ +++ G + T+++D+KEL++ +VP + + Sbjct: 1 MNKGQRHIKIREIITANEIETQDELVDILKKD-GYNVTQATVSRDIKELHLVKVPTNNGS 59 Query: 78 WIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSPGFAQGINYFIDQLNIEEI 137 + Y ++ K + + I++KT PG AQ I +D L+ EEI Sbjct: 60 YKY-SLPADQRFNPLSKLKRSLMDAFVKIDSASHLIVLKTMPGNAQAIGALMDNLDWEEI 118 Query: 138 LGTVSGNDTTLILTASNDMAEYVYAKL 164 +GT+ G+DT LI+ ++D + V K+ Sbjct: 119 MGTICGDDTILIICRTHDDTKVVQKKI 145
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019720	THERMOLYSIN	440	e-152	Thermolysin metalloprotease (M4) family signature.
>THERMOLYSIN#Thermolysin metalloprotease (M4) family signature. Length = 544 Score = 440 bits (1133), Expect = e-152 Identities = 173/480 (36%), Positives = 249/480 (51%), Gaps = 42/480 (8%) Query: 64 NIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLING----DTDA 119 + ++ K D+ G T + ++ + H + G++ ++G + D Sbjct: 71 QARERLSLIGNKLDELGHTVMRFEQAIAASLCMGAVLVAHVN-DGELSSLSGTLIPNLDK 129 Query: 120 KKVKPTNKVTLSKDDAADKAFKAVKIDKHKAKNLKDKVIKENKVEIDGDSNKYVYNVELI 179 + +K +++ + + K A ++ K + ++ + D ++ + Y V + Sbjct: 130 RTLKTEAAISIQQAEMIAKQDVADRVTKERPAA-EEGKPTRLVIYPDEETPRLAYEVNVR 188 Query: 180 TVTPEISHWKVKIDAQTGEILEKMNLVKEA-----------AETGKGKGVLGDTKDINI- 227 +TP +W IDA G++L K N + EA + G G+GVLGD K IN Sbjct: 189 FLTPVPGNWIYMIDAADGKVLNKWNQMDEAKPGGAQPVAGTSTVGVGRGVLGDQKYINTT 248 Query: 228 -NSIDGGFSLEDLTHQGKLSAFSFNDQTG-QATLITNEDENFVKDEQRAGVDANYYAKQT 285 +S G + L+D T + + ++T +L + D F A VDA+YYA Sbjct: 249 YSSYYGYYYLQDNTRGSGIFTYDGRNRTVLPGSLWADGDNQFFASYDAAAVDAHYYAGVV 308 Query: 286 YDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSG 345 YDYYK+ GR SYD + I S H YG NNA W G +M+YGDGDG+TF SG Sbjct: 309 YDYYKNVHGRLSYDGSNAAIRSTVH---YG--RGYNNAFWNGSQMVYGDGDGQTFLPFSG 363 Query: 346 ANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVD-----DEDFLMGEDVYTP 400 DVV HELTH VT TA L Y+++SGA+NE+ SD+FG V+ + D+ +GED+YTP Sbjct: 364 GIDVVGHELTHAVTDYTAGLVYQNESGAINEAMSDIFGTLVEFYANRNPDWEIGEDIYTP 423 Query: 401 GKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQ--------- 451 G GDALRSMS+P ++G P H +DNGGVHTNSGI NKAAY + Q Sbjct: 424 GVAGDALRSMSDPAKYGDPDHYSKRYTGTQDNGGVHTNSGIINKAAYLLSQGGVHYGVSV 483 Query: 452 -AIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAE--QVYEAWNEVGV 508 IG+ K +I+YRAL YLT SNF + A QAA DLY + E V +A+N VGV Sbjct: 484 TGIGRDKMGKIFYRALVYYLTPTSNFSQLRAACVQAAADLYGSTSQEVNSVKQAFNAVGV 543

SAI8T7_1019780

SAI8T7_1019930

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
SAI8T7_1019780	-1	9	0.060528	Similar to phage infection protein
SAI8T7_1019790	1	11	-0.143239	Similar to autolysin
SAI8T7_1019800	1	16	0.009697	Isochorismatase transposase
SAI8T7_1019810	0	14	0.319629	Putative Cell-wall-anchored protein SasF
SAI8T7_1019820	0	15	0.707440	Putative uncharacterized protein
SAI8T7_1019830	0	13	0.032032	Similar to lipopolysaccharide biosynthesis
SAI8T7_1019840	7	13	2.228436	Protein translocase subunit SecA 2
SAI8T7_1019850	9	14	2.631603	Putative uncharacterized protein
SAI8T7_1019860	9	14	2.789941	Putative uncharacterized protein
SAI8T7_1019870	11	15	2.787090	Putative uncharacterized protein
SAI8T7_1019880	11	16	2.981833	Accessory Sec system protein translocase subunit
SAI8T7_1019890	12	17	3.725961	Serine-rich adhesin for platelets
SAI8T7_1019900	-1	17	1.320632	Putative uncharacterized protein
SAI8T7_1019910	-2	15	-0.438092	Putative uncharacterized protein
SAI8T7_1019920	-1	16	-1.627754	Similar to methionine sulfoxide reductase
SAI8T7_1019930	-1	17	-1.200076	Putative acetyltransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019780	ABC2TRNSPORT	39	6e-05	ABC-2 type transport system membrane protein signat...
>ABC2TRNSPORT#ABC-2 type transport system membrane protein signature. Length = 262 Score = 39.1 bits (91), Expect = 6e-05 Identities = 37/172 (21%), Positives = 67/172 (38%), Gaps = 28/172 (16%) Query: 817 NKHKSLESVLTTRQVFLGKAGFFIMLGML-----QALIVSVGDLLILKAGVESP---VLF 868 ++ E++L T Q+ LG I+LG + +A + G ++ A + +L+ Sbjct: 95 EGQRTWEAMLYT-QLRLGD----IVLGEMAWAATKAALAGAGIGVVAAALGYTQWLSLLY 149 Query: 869 VLITI-FCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAG----GGGTFPIQTTPQFFQ 923 L I + F S+ +L P I L I G FP+ P FQ Sbjct: 150 ALPVIALTGLAFASLGMVVTAL--APSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQ 207 Query: 924 NISPYLPFTYAIDSLRETV-----GGIVPEILITKLIILTLFGIGFFVVGLI 970 + +LP +++ID +R + + + + I+ F F L+ Sbjct: 208 TAARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPF---FLSTALL 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019790	FLGFLGJ	64	5e-13	Flagellar protein FlgJ signature.
>FLGFLGJ#Flagellar protein FlgJ signature. Length = 313 Score = 63.6 bits (154), Expect = 5e-13 Identities = 50/176 (28%), Positives = 84/176 (47%), Gaps = 19/176 (10%) Query: 304 SNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSALAKS 363 N DDS D++ F+ ++ A Q + + +++AQA LES G+ + + Sbjct: 139 RNYDDSLPG------DSKAFLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRE 192 Query: 364 ---PNHNLFGIK--GAFEGNSVPFNTLEADGNKLYSINAGFRKYPSTKESLKDYSDLIKN 418 P++NLFG+K G ++G T E + + + A FR Y S E+L DY L+ Sbjct: 193 NGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTR 252 Query: 419 GIDGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDE 474 + + A + +DA YATDP+YA+KL ++I+ Q+ D+ Sbjct: 253 NPRYAAVTTAASAEQGAQALQDA------GYATDPHYARKLTNMIQ--QMKSISDK 300
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019800	ISCHRISMTASE	77	3e-19	Isochorismatase signature.
>ISCHRISMTASE#Isochorismatase signature. Length = 312 Score = 77.0 bits (189), Expect = 3e-19 Identities = 41/183 (22%), Positives = 77/183 (42%), Gaps = 10/183 (5%) Query: 5 RKTALLVLDMQE----GIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDV 60 + LL+ DMQ + + + ++ Q IPV++ ++ +D Sbjct: 29 NRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPGSQNPDDR 88 Query: 61 SSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRAND 120 + + G + +I+ +LAP +D+ +++K R+SAF + L +R Sbjct: 89 ALLTDFW------GPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEG 142 Query: 121 INHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDKHDFIIEQILSRSCDIES 180 + L++TG+ L TA E+ +D + DAV D S +KH +E R Sbjct: 143 RDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTVM 202 Query: 181 VES 183 +S Sbjct: 203 TDS 205
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019840	SECA	648	0.0	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 648 bits (1672), Expect = 0.0 Identities = 282/823 (34%), Positives = 441/823 (53%), Gaps = 68/823 (8%) Query: 2 KRINTWSDEVKSYSDDALKQKTIEFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPK 61 IN E++ SD+ LK KT EF+ RL G + L+ L+PEA+AV REAS RV GM Sbjct: 26 NIINAMEPEMEKLSDEELKGKTAEFRARLEKG-EVLENLIPEAFAVVREASKRVFGMRHF 84 Query: 62 EVQLIGAIVLHEGNIAEMQTGEGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQ 121 +VQL+G +VL+E IAEM+TGEGKTLTAT+P YLNAL+GKG +++T NDYLA+RD E + Sbjct: 85 DVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNR 144 Query: 122 PLYEWLGLTASLGFVDIVDYEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKF 181 PL+E+LGLT V I KR Y DI Y TN GFDYL DN+A S E + Sbjct: 145 PLFEFLGLT-----VGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERV 199 Query: 182 LPQLNYGIIDEVDSIILDAAQTPLVISGAPRLQSNLFHIVKEFVDTLIE----------- 230 +L+Y ++DEVDSI++D A+TPL+ISG S ++ V + + LI Sbjct: 200 QRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQG 259 Query: 231 DVHFKMKKTKKEIWLLNQGIEAAQSYFNV-------EDLYSEQAMVLVRNINLALRAQYL 283 + HF + + +++ L +G+ + E LYS ++L+ ++ ALRA L Sbjct: 260 EGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHAL 319 Query: 284 FESNVDYFVYNGDIVLIDRITGRMLPGTKLQAGLHQAIEAKEGMEVSTDKSVMATITFQN 343 F +VDY V +G+++++D TGR + G + GLHQA+EAKEG+++ + +A+ITFQN Sbjct: 320 FTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQN 379 Query: 344 LFKLFESFSGMTATGKLGESEFFDLYSKIVVQAPTDKAIQRIDEPDKVFRSVDEKNIAMI 403 F+L+E +GMT T EF +Y V PT++ + R D PD V+ + EK A+I Sbjct: 380 YFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAII 439 Query: 404 HDIVELHETGRPVLLITRTAEAAEYFSKVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGS 463 DI E G+PVL+ T + E +E S L + I +N+L A+ A EA ++A+AG + Sbjct: 440 EDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAA 499 Query: 464 MTVATSMAGRGTDIKLG-----------------------------EGVEALGGLAVIIH 494 +T+AT+MAGRGTDI LG + V GGL +I Sbjct: 500 VTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGT 559 Query: 495 EHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYSLDAQRLSQ 554 E E+ R+D QLRGRSGRQGD GSS Y+S++D L++ ++ ++ + + + Sbjct: 560 ERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIE 619 Query: 555 SNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNRVLEIDDAENR 614 + + AQR E + R+ E++ + QR +Y +RN +L++ D Sbjct: 620 HPWVTKAIA----NAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSET 675 Query: 615 DFKALAKDVFEMFVNEE---KVLTKSRVVEYIYQNLSFQFNKDVACVNFKDKQAVVT--- 668 ++ +DVF+ ++ + L + + + + L F+ D+ + DK+ + Sbjct: 676 -INSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEET 734 Query: 669 ---FLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKASVNQRQ 725 +L Q + ++ + A F + V L+ +DS W E + + L+ ++ R Sbjct: 735 LRERILAQSIEVYQ-RKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRG 793 Query: 726 NGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKE 768 Q++ EY R + F M ++K ++ + + + +E Sbjct: 794 YAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEE 836
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019880	SECYTRNLCASE	128	2e-35	Preprotein translocase SecY subunit signature.
>SECYTRNLCASE#Preprotein translocase SecY subunit signature. Length = 437 Score = 128 bits (324), Expect = 2e-35 Identities = 93/440 (21%), Positives = 181/440 (41%), Gaps = 52/440 (11%) Query: 4 LLQQYEYKIIYKRMLYTCFILFIYILGTNISI--VSYNDMQ------VKHESFFKIAISN 55 + + + K++L+T I+ +Y +GT+I I V Y ++Q ++ F + Sbjct: 5 FARAFRTPDLRKKLLFTLAIIVVYRVGTHIPIPGVDYKNVQQCVREASGNQGLFGLVNMF 64 Query: 56 MGGDVNTLNIFTLGLVPWLTSMIILMLISYRNMDKYMKQTSLEKHYKE------------ 103 GG + + IF LG++P++T+ IIL L++ + LE KE Sbjct: 65 SGGALLQITIFALGIMPYITASIILQLLT-------VVIPRLEALKKEGQAGTAKITQYT 117 Query: 104 RILTLILSVIQSYFVIHEYVSKERVHQDN-------------IYLTILILVTGTILLVWL 150 R LT+ L+++Q ++ S + + ++ + GT +++WL Sbjct: 118 RYLTVALAILQGTGLVATARSAPLFGRCSVGGQIVPDQSIFTTITMVICMTAGTCVVMWL 177 Query: 151 ADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYI------DASHIVIALLIILVIITLFILL 204 + + GI M I+M I + + I I +I + +I + +++ Sbjct: 178 GELITDRGIGNGMSILMFISIAATFPSALWAIKKQGTLAGGWIEFGTVIAVGLIMVALVV 237 Query: 205 FIELVEVRIPYI----DLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFIL 260 F+E + RIP + S +Y+ KVN AG I ++ + S F Sbjct: 238 FVEQAQRRIPVQYAKRMIGRRSYGGTSTYIPLKVNQAGVIPVIFASSLLYIPALVAQFAG 297 Query: 261 SMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGV 320 + + D P+ I Y ++ + +F N ++ + + K G + G+ Sbjct: 298 GNSGWKSWVEQNLTKGDHPIYIVTYFLLIVFFAFFYVAISFNPEEVADNMKKYGGFIPGI 357 Query: 321 KPGKDTERYLNYQARRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFS-VQLIVLVYIS 379 + G+ T YL+Y R+ W GS + +I +P L S F ++++V + Sbjct: 358 RAGRPTAEYLSYVLNRITWPGSLYLGLIALVP-TMALVGFGASQNFPFGGTSILIIVGVG 416 Query: 380 INIAETIRTYLYFDKYKPFL 399 + + I + L Y+ FL Sbjct: 417 LETVKQIESQLQQRNYEGFL 436
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019890	ICENUCLEATIN	55	3e-09	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 55.1 bits (132), Expect = 3e-09 Identities = 237/1070 (22%), Positives = 425/1070 (39%), Gaps = 12/1070 (1%) Query: 1098 SDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSVSD 1157 + + ++ + S S + + T +T S ST ++ + Sbjct: 106 LHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQTIEIATYG 165 Query: 1158 STSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQSASAFLSESLSE 1217 ST T +S I+ ST + + + S + ++ST + S ES Sbjct: 166 STLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQM 225 Query: 1218 STSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKSE 1277 + ST + S + S T+ S+ + ST + S+ T+ ST T + Sbjct: 226 AGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 285 Query: 1278 SVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSL 1337 S T+ ST T+ ++S+ ++ S T+ +S + ST + S + ST Sbjct: 286 SDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGT 345 Query: 1338 SGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQ 1397 +G S + S+ + DS+ + S T+ S T+ S T+ + S + Sbjct: 346 AGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYG 405 Query: 1398 SGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQ 1457 S + S + ST T++ S T+ Y S T+ +S+ + S T+ S+ Sbjct: 406 STQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLT 465 Query: 1458 SGSTSTSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSA 1517 +G ST + GS+ + S ST+ ES+ + S + S + ST + + Sbjct: 466 AGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNE 525 Query: 1518 SASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSE 1577 S + STS + + S+ + S ++ S+ + ST + ST Sbjct: 526 SDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGT 585 Query: 1578 SGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSD 1637 +GS S + ST T+ S T+ S + S T+ STS + + S + Sbjct: 586 AGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYG 645 Query: 1638 SQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVM 1697 S + S T+ ST + SD T+ S ST+G+ S I+ ST T+ S + Sbjct: 646 STQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 705 Query: 1698 SASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESS 1757 + S + S S S S + +DS ++G S + S T+ S + Sbjct: 706 AGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQ 765 Query: 1758 SLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTST 1817 S+ + S S + +DSS ++ S +++ S + S T+ S T+G STST Sbjct: 766 SVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTST 825 Query: 1818 SLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQ 1877 + + S ++ S + S T+ S + S + STS + DS ++ Sbjct: 826 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYG 885 Query: 1878 SMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSD 1937 S + S + S+ T+ D + S S + S GS T++ ST Sbjct: 886 STQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLM 945 Query: 1938 SMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSIST 1997 + GS + S + GSTS++ S+ + S + QST T+ GS T+ + Sbjct: 946 AGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQTAEHS 1005 Query: 1998 SMSMSASTSSSQSTSVSTSLSTSDSISDST----------SISISGSQSTVESESTSDST 2047 S + S++ + + S+ ++ S S S ISG +S + + S Sbjct: 1006 STLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGYGSSLI 1065 Query: 2048 SISDSESLSTSDSDSTSTSTSDSTSGSTSTSIS--ESLSTSGSGSTSVSDSTSMSESDST 2105 S S + S+ ++ S +G ST I+ S+ +G GS+ + S S + Sbjct: 1066 SGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGAD 1125 Query: 2106 SVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSESLSTSMSGSQSI 2155 SV M+ ++ + +DS + S L+ ++S T+ S +G+ I Sbjct: 1126 SVQMAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDCI 1175 Score = 53.2 bits (127), Expect = 1e-08 Identities = 176/773 (22%), Positives = 305/773 (39%), Gaps = 2/773 (0%) Query: 1408 SASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSTSTSASL 1467 + + +E + S + + T D+T S ST + + + Sbjct: 106 LHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQTIEIATYG 165 Query: 1468 SGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASESDSSST 1527 S SQ I+ S T+ +ST ++ ST +G+ ST + S + +SS Sbjct: 166 STLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQM 225 Query: 1528 SLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSE 1587 + ST M+ S+ + S + S+ + ST + S T+ GST + Sbjct: 226 AGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 285 Query: 1588 SDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTST 1647 SD T+ S + + S+ +G ST T+ +S T+ ST SD + ST T Sbjct: 286 SDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGT 345 Query: 1648 STSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSM 1707 + S + S + DS+ + GS + SD T+ S + S + Sbjct: 346 AGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYG 405 Query: 1708 SESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSD 1767 S ES + S + GS + GS + + S+ +G S Sbjct: 406 STQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLT 465 Query: 1768 SVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSE 1827 + S ++ S S S + S + GST T+ GS T+ S + +E Sbjct: 466 AGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNE 525 Query: 1828 STSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTST 1887 S ++ S S + + S + GS + S+ T+ S + S +G ST T Sbjct: 526 SDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGT 585 Query: 1888 SVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGSVSVST 1947 + SDS + S + S+ + ST + S + S ST+ + S ++ Sbjct: 586 AGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYG 645 Query: 1948 STSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSS 2007 ST + S T+ S+ T+ S + S ST+ + S ++ ST + S + Sbjct: 646 STQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 705 Query: 2008 SQSTSVSTSLSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTST 2067 + S T+ SD S S S +G+ S++ + S T+ S + S T+ Sbjct: 706 AGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQ 765 Query: 2068 SDSTS--GSTSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESV 2125 S T+ GSTST+ ++S +G GST + S+ + S +Q++SD T+ S S Sbjct: 766 SVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTST 825 Query: 2126 STSTSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPS 2178 + + S+ ++ ST T+ S +G S + S + S S + + S Sbjct: 826 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDS 878 Score = 53.2 bits (127), Expect = 1e-08 Identities = 233/1050 (22%), Positives = 411/1050 (39%), Gaps = 2/1050 (0%) Query: 759 TSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKSTSVSLSDSVSASKSLSTSES 818 TS + A ++ + S + D+ S S +++ Sbjct: 99 TSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQT 158 Query: 819 NSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNSNSTEKSESLSTSTSDSLRTSTS 878 +++ ST QS + GS + S I+ ST + + ST + T T+ Sbjct: 159 IEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTA 218 Query: 879 LSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDSTSNAISTSTSLSESASTSDSISIS 938 +S M+ GS S +G ST + DS+ A ST + S+ + S Sbjct: 219 GEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 278 Query: 939 NSIANSQSASTSKSDSQSTSISLSTSDSKSMSTSESLSDSTSTSGSVSGSLSIAASQSVS 998 A S T+ S T+ + S+ + ST + +ST T+G GS A S Sbjct: 279 TQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGY--GSTQTAQKGSDL 336 Query: 999 TSTSDSMSTSEIVSDSISTSGSLSASDSKSMSVSSSMSTSQSGSTSESLSDSQSTSDSDS 1058 T+ S T+ S I+ GS + S + ST + S+ + ST + + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 1059 KSLSLSTSQSGSTSTSTSTSASVRTSESQSTSGSMSASQSDSMSISTSFSDSTSDSKSAS 1118 S ++ S T+ ST + S + GS + S + S + S Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 456 Query: 1119 TASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSVSDSTSLSTSESDSISESTSTSDS 1178 TA +S + ST + S + S T+ S + S + ST T+ Sbjct: 457 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGY 516 Query: 1179 ISEAISASESTSISLSESNSTSDSESQSASAFLSESLSESTSESTSESVSSSTSESTSLS 1238 S + +ES I+ S ST+ + S + + S + S T+ S+ T+ S Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDL 576 Query: 1239 DSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKSESVSTSLSMSTSTSLSNSTSLS 1298 + S T+ S S+ +G S T++ S T+ + S + S+ T+ S ST+ + Sbjct: 577 TAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGA 636 Query: 1299 TSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDS 1358 S + S + S+ T+ ST + S T+ GSTS + +DS+ + S Sbjct: 637 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQ 696 Query: 1359 TSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTS 1418 T+ S+ + GST T+ S S S S + + + S ++ +S+ T+ Sbjct: 697 TAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGY 756 Query: 1419 ESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQS 1478 S + + S ST+ + S + S T+ S T+ S ++ S Sbjct: 757 GSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDL 816 Query: 1479 ISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQ 1538 + S ST+ + S+ ++ ST +G S T+ S ++ +S T+ STS + Sbjct: 817 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 876 Query: 1539 SSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDS 1598 S + S + S T+ ST + S T+ GSTS + ES + S Sbjct: 877 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQ 936 Query: 1599 QSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLS 1658 ++ +ST +G S+ T+ S T+ STSM S + ST T+ S T+ Sbjct: 937 TASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGY 996 Query: 1659 DSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVS 1718 S + ST + GS + + + S + S+ S + S ++ SV Sbjct: 997 GSTQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVL 1056 Query: 1719 ESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLS 1778 + S S S+ + GS +++ + ES+ ++G++SM + S ++ Sbjct: 1057 TAGYGSSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGY 1116 Query: 1779 VSTSLRSSESVSESDSLSDSKSTSGSTSTS 1808 ST + ++SV + + + ST T+ Sbjct: 1117 RSTLISGADSVQMAGERGKLIAGADSTQTA 1146 Score = 52.8 bits (126), Expect = 2e-08 Identities = 229/1007 (22%), Positives = 395/1007 (39%), Gaps = 10/1007 (0%) Query: 1163 TSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQSASAFLSESLSESTSES 1222 TS I + + +E + S ++ ES S +++ Sbjct: 99 TSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQT 158 Query: 1223 TSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKSESVSTS 1282 + ST T S + GST T+ +ST + ST T+ ++ST S T+ Sbjct: 159 IEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTA 218 Query: 1283 LSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTSLSGSTS 1342 S+ + ST SD T+ S + S+ + S + S+ +G S Sbjct: 219 GEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 278 Query: 1343 ESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMNQSGVDS 1402 + S + ST + + S +G ST T+ S T+ S + S + + Sbjct: 279 TQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTA 338 Query: 1403 NSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTSQSGSTS 1462 S + S+ + S T+ S T+ ST T+ SD T+ GST Sbjct: 339 GYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTA------GYGSTG 392 Query: 1463 TSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGSASTSTSLSNSASASES 1522 T+ + S + S + S T+ ST + S +G ST T+ +S+ + Sbjct: 393 TAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGY 452 Query: 1523 DSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVSTSESGSTS 1582 S+ T+ DS+ + S +Q S + STST+ S++ ++ ST +G S Sbjct: 453 GSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSL--IAGYGSTQTAGYGS 510 Query: 1583 ESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTSTSDSQSMS 1642 T+ ST T+ ++S + S S + + S+ + ST ++ S+ T+ S + Sbjct: 511 TLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTA 570 Query: 1643 LSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVSISLSDSTSTSTSASEVMSASIS 1702 S T+ ST + S S + S T+ S + ST T+ S + + S Sbjct: 571 REGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGS 630 Query: 1703 DSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGS 1762 S + ++S + S + +S +G S + S T+ S S + + S + Sbjct: 631 TSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIA 690 Query: 1763 QSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGS 1822 S + +S + S ++++ S+ S S ST+G+ S+ +G ST T+ S Sbjct: 691 GYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHS 750 Query: 1823 ESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGS 1882 + S + S T+ S S +G+ S + ST + S + S + Sbjct: 751 SLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTA 810 Query: 1883 ESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGS 1942 + S + S+ST+ DS I+ S + +S +G ST T+ SD +G Sbjct: 811 QERSDLTTGYGSTSTAG--ADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGY 868 Query: 1943 VSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMS 2002 S ST+ S I+G S + S T+ S +Q S +G STS + S Sbjct: 869 GSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSL 928 Query: 2003 ASTSSSQSTSVSTSLSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDS 2062 + S T+ S + S T+ S + S S + S + ST + Sbjct: 929 IAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGY 988 Query: 2063 TSTSTSDSTSGSTSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDS 2122 ST T+ S T+ S + GS +T+ +DS+ ++ S+ S + + S Sbjct: 989 QSTLTAGYGSTQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTL 1048 Query: 2123 ESVSTSTSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTST 2169 S S T+ S S S T+ GS I+ S+ ++G ST Sbjct: 1049 ISGLRSVLTAGYGSSLISGRRSSLTAGYGSNQIASHRSSLIAGPEST 1095 Score = 52.1 bits (124), Expect = 3e-08 Identities = 193/856 (22%), Positives = 350/856 (40%), Gaps = 14/856 (1%) Query: 1329 DSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTS 1388 S + ++ + + + S + + + + T +T S ST + Sbjct: 97 TKTSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPT 156 Query: 1389 LSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDS 1448 ++ + S + SQ + ST T+ S + Y S T+ ++ST + S Sbjct: 157 QTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQ 216 Query: 1449 TSISKSTSQSGSTSTSASLSGSESESDSQSISTSASEST--SESASTSLSDSTSTSNSGS 1506 T+ +S+ +G ST + GS+ + S T+ +S+ + ST + S+ +G Sbjct: 217 TAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGY 276 Query: 1507 ASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTI 1566 ST T+ S + S+ T+ +DS+ + S + S + ST T+ + S + Sbjct: 277 GSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDL 336 Query: 1567 ASLSTSVSTSESGST------SESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDS 1620 + S T+ S+ S T+ DS+ T+ S T++ S + ST T+ + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 1621 RSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVS 1680 S+ + S +T+ +S + ST T+ S + S T+ S+ +G S Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 456 Query: 1681 ISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDSGS 1740 + DS+ T+ S + SD + S + + S + S +G S +G Sbjct: 457 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGY 516 Query: 1741 LSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDSKS 1800 S T+ +S+ ++ S S + + S ++ S+ + S+ ++ S + S Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDL 576 Query: 1801 TSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGSTS 1860 T+G ST T+GS S+ + GS + S + S T+ S +G S S + + Sbjct: 577 TAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGA 636 Query: 1861 LST------SDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDS 1914 S+ S + S+ ++ S + S + STS + DS I+ S Sbjct: 637 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQ 696 Query: 1915 MSTSDSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLS 1974 + +S +G ST T+ SD SG S ST+ + S I+G S +S S+ T+ Sbjct: 697 TAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGY 756 Query: 1975 DSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTSLSTSDSISDSTSISISGS 2034 S ++ S +G STS + + S + S T+ S+ T+ S T+ S Sbjct: 757 GSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDL 816 Query: 2035 QSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGSTSTSISESLSTSGSGSTSVS 2094 + S ST+ + S + ST + S T+ S T+ S+ + GS ST+ Sbjct: 817 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 876 Query: 2095 DSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSTSLSTSDSTSTSESLSTSMSGSQS 2154 DS+ ++ ST + + S + S T+ S ST+ ES + GS Sbjct: 877 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQ 936 Query: 2155 ISDSTSTSMSGSTSTS 2170 + ST M+G S+ Sbjct: 937 TASFKSTLMAGYGSSQ 952 Score = 51.3 bits (122), Expect = 5e-08 Identities = 200/886 (22%), Positives = 350/886 (39%), Gaps = 6/886 (0%) Query: 797 KSTSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS 856 ++ + + SA + + ++ V+ + + S V+S + D + Sbjct: 89 RAEVLHVGTKTSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATI 148 Query: 857 NSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDSTS 916 S + + + T + S ++ GS + ST I+G ST + +DST Sbjct: 149 ESGSTQPTQTIEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTL 208 Query: 917 NAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTSESLS 976 A ST + S+ + S S T+ S T+ S+ + ST + Sbjct: 209 VAGYGSTQTAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGE 268 Query: 977 DSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMSVSSSMS 1036 DS+ T+G S + S + S + ++ + S + +S + S Sbjct: 269 DSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQ 328 Query: 1037 TSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQSTSGSMSAS 1096 T+Q GS + S T+ DS + + GST T+ S+ S T+ S Sbjct: 329 TAQKGSDLTAGYGSTGTAGDDSSLI----AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDL 384 Query: 1097 QSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTSNSERTSTSVS 1156 + S T+ +DS+ + ST ++ S + S + S T+ T T+ Sbjct: 385 TAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGD 444 Query: 1157 DSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQSASAFLSESLS 1216 DS+ ++ S + S+ + + ++ S + STS + +S+ S Sbjct: 445 DSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQ 504 Query: 1217 ESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTSISESTSTFKS 1276 + ST + ST + + SD + GSTST+ +NS+ + ST T+ S T Sbjct: 505 TAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGY 564 Query: 1277 ESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTSKSDSISTSTS 1336 S T+ S T+ ST + S S + S ++ S+ + S + S Sbjct: 565 GSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVL 624 Query: 1337 LSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTSTSLSLSASMN 1396 +G S S + + SS + ST + S T+G ST T+ SD T+ S S + Sbjct: 625 TTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGA 684 Query: 1397 QSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLSDSTSISKSTS 1456 S + + S + S T+ S T+ S TS STST+ + S + ST Sbjct: 685 DSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQ 744 Query: 1457 QSGSTSTSASLSGSESESDSQSISTS--ASESTSESASTSLSDSTSTSNSGSASTSTSLS 1514 + S+ + GS + QS+ T+ S ST+ + S+ ++ ST +G S T+ Sbjct: 745 TASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGY 804 Query: 1515 NSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTIASLSTSVS 1574 S ++ S T+ STS + S + S + S T+ ST + S Sbjct: 805 GSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDL 864 Query: 1575 TSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDSRSTSASTSTSMRTS 1634 T+ GSTS + +S + S + S +G ST T+ +S T+ STS Sbjct: 865 TTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGY 924 Query: 1635 TSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMSVS 1680 S + ST T++ S + S + S+ + GS S++ Sbjct: 925 ESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMA 970 Score = 49.0 bits (116), Expect = 3e-07 Identities = 206/894 (23%), Positives = 361/894 (40%), Gaps = 6/894 (0%) Query: 733 TDASGNKTTTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTS 792 T G+ T + T + S ++ GSTQ + ST A S T+ GS + + Sbjct: 281 TAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGY 340 Query: 793 ASTSKSTSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDS 852 ST + S + S + +S+ + ST S ++ GS + + S Sbjct: 341 GSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSL 400 Query: 853 ISNSNSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLS 912 I+ ST+ + ST T+ T T+ S + GS + S+ I+G ST + Sbjct: 401 IAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGE 460 Query: 913 DSTSNAISTSTSLSESASTSDSISISNSIANSQSA------STSKSDSQSTSISLSTSDS 966 DS+ A ST ++ S + S S A +S+ ST + ST + S Sbjct: 461 DSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQ 520 Query: 967 KSMSTSESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDS 1026 + + S+ ++ STS + + S IA S T++ +S+ T+ S + GS + Sbjct: 521 TAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGY 580 Query: 1027 KSMSVSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSES 1086 S + S S+ +G S + S+ + S + QS T+ STS + S Sbjct: 581 GSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSL 640 Query: 1087 QSTSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS 1146 + GS + +S+ + S T+ S TA S S + + S+ + ST + Sbjct: 641 IAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGY 700 Query: 1147 NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQS 1206 NS T+ S T+ S+ S STST+ + S I+ ST + S+ T+ S Sbjct: 701 NSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQ 760 Query: 1207 ASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTS 1266 + S + S ST+ + SS + S + S T+ S T+ S T+ Sbjct: 761 TAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGY 820 Query: 1267 ISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTS 1326 S ST+ S ++ S T+ S T+ S + +S + S ST+ S+ Sbjct: 821 GSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSL 880 Query: 1327 KSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDSTS 1386 + ST T+ S + ST +++ SD T+ S S + S+ + S ++ Sbjct: 881 IAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASF 940 Query: 1387 TSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSESTSTSTSLS 1446 S ++ + S+ + STS + +S + T + QS T+ S Sbjct: 941 KSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQ 1000 Query: 1447 DSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTSLSDSTSTSNSGS 1506 + S T+ GST+T+ + S + S S S T+ ST +S S +G Sbjct: 1001 TAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGY 1060 Query: 1507 ASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTSTSNRMSTI 1566 S+ S S+ + S+ + S+ + S + + S ++ S+ T+ ST+ Sbjct: 1061 GSSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTL 1120 Query: 1567 ASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTSDS 1620 S + SV + + ++S T+ S + + S +G S T+ +D Sbjct: 1121 ISGADSVQMAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDC 1174 Score = 47.8 bits (113), Expect = 6e-07 Identities = 241/1091 (22%), Positives = 433/1091 (39%), Gaps = 10/1091 (0%) Query: 907 TSASLSDSTSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDS 966 TSA A + + ++ S ++ + N T D+ S S + + Sbjct: 99 TSAMQFILHHRADYVACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQT 158 Query: 967 KSMSTSESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDS 1026 ++T S T S ++G S + ST + ST +DS +G S + Sbjct: 159 IEIATYGSTLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTA 218 Query: 1027 KSMSVSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSES 1086 S + S S + S + S + GST T+ S+ S Sbjct: 219 GEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 278 Query: 1087 QSTSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS 1146 T+ S + S T+ +DS+ + ST ++ S + S + S T+ Sbjct: 279 TQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTA 338 Query: 1147 NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSESQS 1206 T T+ DS+ ++ S + S+ + + ++ S + ST + + S Sbjct: 339 GYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADS 398 Query: 1207 ASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASISTSTS 1266 + S + EST + ST + SD T+ GST T+ +S+ + ST T+ Sbjct: 399 SLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTA 458 Query: 1267 ISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMS--TSDSIS 1324 +S+ T S T+ S T+ STS + S + S + S T+ S Sbjct: 459 GEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGS 518 Query: 1325 TSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTS------TS 1378 T + + S + GSTS + ++S+ + S T+ S+ + GST T+ T+ Sbjct: 519 TQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTA 578 Query: 1379 TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQSES 1438 S T+ S S + S ++ S + ST T+ S T+ Y S ST+ ++S Sbjct: 579 GYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADS 638 Query: 1439 TSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTSLSDS 1498 + + S T+ S +G ST + GS+ + S ST+ ++S+ + S + Sbjct: 639 SLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTA 698 Query: 1499 TSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTS 1558 S + ST + S S STS + + S+ + S ++ S + S Sbjct: 699 GYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGS 758 Query: 1559 TSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTSTS 1618 T + STS +G+ S + ST T+ S T+ S + S T+ Sbjct: 759 TQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTT 818 Query: 1619 DSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTSGSMS 1678 STS + + S + S + S T+ ST + SD T+ S ST+G S Sbjct: 819 GYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDS 878 Query: 1679 VSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGSTSVSDS 1738 I+ ST T+ S + + S + S + S S + +S ++G S + Sbjct: 879 SLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTA 938 Query: 1739 GSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVSESDSLSDS 1798 S + S + S + S S++ DSS ++ S +++ S + S Sbjct: 939 SFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGS 998 Query: 1799 KSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDSLSGSISLSGS 1858 T+ +ST T+G ST+T+ + S ++ S S S T+ S +SG S+ + Sbjct: 999 TQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTA 1058 Query: 1859 TSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMSISASESDSMSTS 1918 S+ S S + S + S+ ++ +S+ + ++ SM I+ S + Sbjct: 1059 GYGSSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNR--SMLIAGKGSSQTAGY 1116 Query: 1919 DSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDSSSTSTSTSLSDSMS 1978 S+ ISG++S + ++G+ S T+ S ++G+ S + S T+ +D + Sbjct: 1117 RSTLISGADSVQMAGERGKLIAGADSTQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDCIL 1176 Query: 1979 QSQSTSTSASG 1989 + S +G Sbjct: 1177 MAGDRSKLTAG 1187
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019910	NUCEPIMERASE	27	0.043	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 27.4 bits (61), Expect = 0.043 Identities = 9/32 (28%), Positives = 14/32 (43%) Query: 23 IPRPIAFVTTLNQDASVNAAPFSFFNIVNNHP 54 IP T + + AP+ +NI N+ P Sbjct: 234 IPHADTQWTVETGTPAASIAPYRVYNIGNSSP 265
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
SAI8T7_1019930	SACTRNSFRASE	45	1e-08	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 45.3 bits (107), Expect = 1e-08 Identities = 24/101 (23%), Positives = 46/101 (45%), Gaps = 5/101 (4%) Query: 48 EKNDEVIGYIN--GPVIKERYISDDLFKNVSINNSEGGYISVLGLVVAPNYQGQGIAGRL 105 E +D + Y+ G Y+ ++ + I ++ GY + + VA +Y+ +G+ L Sbjct: 51 EDDDMDVSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTAL 110 Query: 106 LNYFETLAKNHHRHGVTLTCRE---SLISFYEKYGYRNEGV 143 L+ AK +H G+ L ++ S FY K+ + V Sbjct: 111 LHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAV 151


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