PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genome2736.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_009783 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1VIBHAR_00182VIBHAR_00188Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00182228-0.454524transposase
VIBHAR_00183334-3.463465transposase
VIBHAR_00184546-6.204923transposase
VIBHAR_00185545-6.129485transposase
VIBHAR_00186545-6.129485transposase
VIBHAR_00187544-5.940626transposase
VIBHAR_00188025-3.805095transposase
2VIBHAR_00279VIBHAR_00285Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_002792181.287543ABC transporter ATPase
VIBHAR_002801171.101119signal recognition particle GTPase
VIBHAR_00281325-0.610234N6-adenine-specific methylase
VIBHAR_00282326-1.241415hypothetical protein
VIBHAR_00283227-2.427269antibiotic transporter
VIBHAR_00284229-3.691684hypothetical protein
VIBHAR_00285327-2.912702hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00280IGASERPTASE493e-08 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 48.5 bits (115), Expect = 3e-08
Identities = 19/86 (22%), Positives = 34/86 (39%), Gaps = 4/86 (4%)

Query: 18 EEQSQKPTTEESVKEEAAEAPAAEQQPTEEVAEQAEEKAAVEAEPEKAEAAPAEAEAEAE 77
E+ Q T + E A+ E + + Q E A +E ++ + + A E
Sbjct: 1052 EKNEQDATETTAQNREVAK----EAKSNVKANTQTNEVAQSGSETKETQTTETKETATVE 1107

Query: 78 AEAEAEAEEPQVAVAPRVQEQEKPTE 103
E +A+ E + P+V Q P +
Sbjct: 1108 KEEKAKVETEKTQEVPKVTSQVSPKQ 1133



Score = 33.5 bits (76), Expect = 0.002
Identities = 21/104 (20%), Positives = 38/104 (36%), Gaps = 15/104 (14%)

Query: 16 GDEEQSQKPTTEESV---KEEAAEAPAAEQQPTEEVAEQAEEKAAVEAEPEKAEAAPAEA 72
+ +++Q T+E+ KEE A+ + Q E + + V + E++E +A
Sbjct: 1090 SETKETQTTETKETATVEKEEKAKVETEKTQ------EVPKVTSQVSPKQEQSETVQPQA 1143

Query: 73 EAEAEAEAEAEAEEPQVAVAPRVQEQEKPTESFFARLKRSLSRT 116
E E + +EPQ Q A+ S
Sbjct: 1144 EPARENDPTVNIKEPQ------SQTNTTADTEQPAKETSSNVEQ 1181



Score = 31.6 bits (71), Expect = 0.006
Identities = 23/91 (25%), Positives = 36/91 (39%), Gaps = 10/91 (10%)

Query: 20 QSQKPTTEESVKEEAA--------EAPAAEQQPTEEVAEQA--EEKAAVEAEPEKAEAAP 69
Q+ P+ + +E A APA + TE VAE + E K + E + E
Sbjct: 1004 QADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTA 1063

Query: 70 AEAEAEAEAEAEAEAEEPQVAVAPRVQEQEK 100
E EA++ +A VA E ++
Sbjct: 1064 QNREVAKEAKSNVKANTQTNEVAQSGSETKE 1094



Score = 30.4 bits (68), Expect = 0.017
Identities = 17/97 (17%), Positives = 32/97 (32%), Gaps = 11/97 (11%)

Query: 19 EQSQKPTTEESVKEEAAE--APAAEQQPTEE----VAEQAEEKAAVEAEPEKAEAAPAEA 72
E +++ ++KE ++ A +QP +E V + E V E
Sbjct: 1144 EPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTT 1203

Query: 73 EAEAEAEAEAEAEE-----PQVAVAPRVQEQEKPTES 104
A + +E+ + +V E T S
Sbjct: 1204 PATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTS 1240



Score = 29.3 bits (65), Expect = 0.038
Identities = 17/83 (20%), Positives = 34/83 (40%), Gaps = 2/83 (2%)

Query: 17 DEEQSQKPTTEESVKEEAAEAPAAEQQPTEEVAEQAEEKAAVEAEPEKAEAAPAEAEAEA 76
++E+ K TE++ + + + +Q E Q + + A E +P +++
Sbjct: 1107 EKEEKAKVETEKTQEVPKVTSQVSPKQEQSE-TVQPQAEPARENDPTVN-IKEPQSQTNT 1164

Query: 77 EAEAEAEAEEPQVAVAPRVQEQE 99
A+ E A+E V V E
Sbjct: 1165 TADTEQPAKETSSNVEQPVTEST 1187


3VIBHAR_00314VIBHAR_00336Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00314316-0.157469porphobilinogen deaminase
VIBHAR_00315218-1.761219uroporphyrinogen-III synthase
VIBHAR_00316219-2.584477uroporphyrin-III C-methyltransferase
VIBHAR_00317328-3.661422heme biosynthesis protein
VIBHAR_00318544-5.940626hypothetical protein
VIBHAR_00319132-4.865642transposase
VIBHAR_00320130-4.293288hypothetical protein
VIBHAR_00321-116-1.592779transposase
VIBHAR_00322-115-1.045310hypothetical protein
VIBHAR_00323-115-0.088521transposase
VIBHAR_00332-1160.198580***FMN reductase
VIBHAR_00333-1160.926720phenol hydroxylase
VIBHAR_00334-2151.4315443-polyprenyl-4-hydrozybenzoate decarboxylase
VIBHAR_003350161.631362hypothetical protein
VIBHAR_003362181.929823transcription termination factor Rho
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00317MICOLLPTASE300.015 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 30.5 bits (68), Expect = 0.015
Identities = 14/93 (15%), Positives = 29/93 (31%), Gaps = 8/93 (8%)

Query: 49 IATLAALFFLEYVVKKVVYASSST--WNYFSVRKM------RRSRRYTNEGIIKLLEGDW 100
I ++ + L V+K + ++ Y R + N+ + +L + W
Sbjct: 672 IKEVSNIKDLSSNVEKSQFFTTYDMRGTYVGGRSQGEENDWKDMNSKLNDILKELSKKSW 731

Query: 101 KGAEKKVTRWANHHDMPLLCYLVASEAAQGQGD 133
G + + NH Y+ D
Sbjct: 732 NGYKTVTAYFVNHKVDGNGNYVYDVVFHGMNTD 764


4VIBHAR_00407VIBHAR_00422Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_004070173.009637hypothetical protein
VIBHAR_00408-1163.439597sensory transduction protein kinase
VIBHAR_00409-1163.687867ATPase
VIBHAR_00410-1173.542126acetolactate synthase 2 catalytic subunit
VIBHAR_00411-1152.688103acetolactate synthase 2 regulatory subunit
VIBHAR_00412-1132.560172branched-chain amino acid aminotransferase
VIBHAR_00413-1112.055592dihydroxy-acid dehydratase
VIBHAR_004141101.672166threonine dehydratase
VIBHAR_004150111.525110putative DNA-binding transcriptional regulator
VIBHAR_004162191.393846inner membrane transport protein YdhC
VIBHAR_004172211.138893prephrenate dehydratase
VIBHAR_004184291.274085amino acid ABC transporter permease
VIBHAR_004194331.071935bifunctional N-acetylglucosamine-1-phosphate
VIBHAR_004205350.813249F0F1 ATP synthase subunit epsilon
VIBHAR_004216360.431955F0F1 ATP synthase subunit beta
VIBHAR_00422231-0.006046F0F1 ATP synthase subunit gamma
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00409HTHFIS433e-06 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 42.5 bits (100), Expect = 3e-06
Identities = 39/193 (20%), Positives = 62/193 (32%), Gaps = 43/193 (22%)

Query: 170 QHQLNLYQTPKKKELKAHGRDLQDIIGQ----QQGKRALEIAAAGNHNLLFLGPPGTGKT 225
+ + +L+ +D ++G+ Q+ R L + L+ G GTGK
Sbjct: 115 IGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKE 174

Query: 226 MLASRLCDLLPEMSDEEAMETASVASLTQSEINEHNWKTRPFRAPH-----HSSSMAALV 280
++A L D + PF A + + L
Sbjct: 175 LVARALHDYGK-------------------------RRNGPFVAINMAAIPRDLIESELF 209

Query: 281 G-------GGSVPRPGEISLAHNGLLFLDEMPEFDRKVLDSLREPLESGEIIISRAQGKT 333
G G G A G LFLDE+ + L L+ GE + G+T
Sbjct: 210 GHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGE--YTTVGGRT 267

Query: 334 RFPARFQIVGALN 346
+ +IV A N
Sbjct: 268 PIRSDVRIVAATN 280


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00416TCRTETB508e-09 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 50.3 bits (120), Expect = 8e-09
Identities = 37/179 (20%), Positives = 75/179 (41%), Gaps = 10/179 (5%)

Query: 8 LVYLAALSMLGFIATDMYLPAFKAMEVDFATGPEQIALSLTVFLVGMAFGQLMWGLASDK 67
L++L LS + + + + DF P T F++ + G ++G SD+
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 68 FGHRNTLAAGLALFTVASFGLAFSEQVWQLLAL-RFVQAIGVCA----PAVIWQAMVIKR 122
G + L G+ + S + LL + RF+Q G A V+ + K
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 123 HSGQSQQIFATIMPLVALSPALAPQLGVLLADSFGWHSIFV--ALTLMGVLLVVATMAQ 179
+ G++ F I +VA+ + P +G ++A W + + +T++ V ++ + +
Sbjct: 136 NRGKA---FGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKK 191


5VIBHAR_00533VIBHAR_00551Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00533015-3.003786hypothetical protein
VIBHAR_00534-118-4.006844ferritin
VIBHAR_00535-118-3.144370universal stress protein UspB
VIBHAR_00536-117-2.890454oxidoreductase
VIBHAR_00537224-4.266790hypothetical protein
VIBHAR_00538122-2.320757hypothetical protein
VIBHAR_00539223-2.765388hypothetical protein
VIBHAR_00540122-1.217799transposase
VIBHAR_00541223-1.844652DNA-directed DNA polymerase
VIBHAR_00542223-2.005076hypothetical protein
VIBHAR_00543116-1.020860hypothetical protein
VIBHAR_00544216-2.015960hypothetical protein
VIBHAR_005451140.194945hypothetical protein
VIBHAR_00546215-0.156358transposase
VIBHAR_00547317-0.580201hypothetical protein
VIBHAR_00548116-0.368885chitinase
VIBHAR_00549326-0.950292hypothetical protein
VIBHAR_00550225-1.198762hypothetical protein
VIBHAR_00551223-0.971538hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00543PYOCINKILLER2088e-60 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 208 bits (530), Expect = 8e-60
Identities = 74/154 (48%), Positives = 99/154 (64%), Gaps = 1/154 (0%)

Query: 706 DAILVFPESSGIAPLYVVYKESPRDKPGVVTGKGEDIFGIWLAGAGKDLGAPIPSQIADK 765
D I+ FP SGI P+YV++++ PRD PG TGKG+ + G WL A + GAPIPSQIADK
Sbjct: 465 DLIIGFPADSGIKPIYVMFRD-PRDVPGAATGKGQPVSGNWLGAASQGEGAPIPSQIADK 523

Query: 766 LRGKEFSSFDAFRKAFWLEVSKDSDLKGQFNAQNQIRIGLGKAPKARSRDKVGKRKSFEL 825
LRGK F ++ FR+ FW+ V+ D +L QFN + + G AP R ++ G R E+
Sbjct: 524 LRGKTFKNWRDFREQFWIAVANDPELSKQFNPGSLAVMRDGGAPYVRESEQAGGRIKIEI 583

Query: 826 HHVDEVQNGGKVYDLDNLKVTTPKNHIDIHRGMK 859
HH V +GG VY++ NL TPK HI+IH+G K
Sbjct: 584 HHKVRVADGGGVYNMGNLVAVTPKRHIEIHKGGK 617


6VIBHAR_00657VIBHAR_00677Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00657-119-3.133227formamidopyrimidine-DNA glycosylase
VIBHAR_00658-221-3.538138hypothetical protein
VIBHAR_00659-121-3.140584phosphopantetheine adenylyltransferase
VIBHAR_00660-123-3.272433glycosyltransferase
VIBHAR_00661-125-3.733606alpha-L-glycero-D-manno-heptose
VIBHAR_00662022-3.883418ADP-heptose--LPS heptosyltransferase III
VIBHAR_00663022-4.049338diacylglycerol kinase
VIBHAR_00664-121-5.0354203-deoxy-D-manno-octulosonic-acid kinase
VIBHAR_00665-121-4.957789glycosyl transferase family protein
VIBHAR_00666-121-5.044955serine acetyltransferase
VIBHAR_00667023-5.723770UDP-N-acetylglucosamine 4,6-dehydratase
VIBHAR_00668028-6.513354UDP-bacillosamine synthetase
VIBHAR_00669230-7.551816hypothetical protein
VIBHAR_00670433-7.921719sialic acid synthase
VIBHAR_00671432-7.879268UDP-N-acetylglucosamine 2-epimerase
VIBHAR_00672635-8.522554hypothetical protein
VIBHAR_00673633-8.418190hypothetical protein
VIBHAR_00674529-6.965335hypothetical protein
VIBHAR_00675327-5.9702573-oxoacyl-ACP synthase
VIBHAR_00676222-4.833897mannose-1-phosphate guanyltransferase
VIBHAR_00677120-4.471431N-acylneuraminate cytidylyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00659LPSBIOSNTHSS2156e-75 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 215 bits (549), Expect = 6e-75
Identities = 70/154 (45%), Positives = 105/154 (68%)

Query: 3 KVIYPGTFDPITNGHLDIITRAANMFDQIIIGVAASPSKKTLFSLEERVKLVEESTAHLS 62
IYPG+FDPIT GHLDII R +FDQ+ + V +P+K+ +FS++ER++ + ++ AHL
Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61

Query: 63 NVSTAGFSGLLVDFAREQEANVLVRGLRTTVDFEYEFGLTSMYRKLLPGVESVFLTPAEE 122
N F GL V++AR+++A ++RGLR DFE E + + + L +E+VFLT + E
Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121

Query: 123 YAFLSSTIVREVAIHGGDVEQFVPKCVYTAIKTK 156
Y+FLSS++V+EVA GG+VE FVP V A+ +
Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHVAAALYDQ 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00666PF05307310.002 Bundlin
		>PF05307#Bundlin

Length = 193

Score = 30.5 bits (68), Expect = 0.002
Identities = 27/83 (32%), Positives = 36/83 (43%), Gaps = 10/83 (12%)

Query: 68 GIVIGSQAKVEKGCRIYQQVTIGSNFDSDNSMAHVSENTYIGSGAKIIGGISIGKNCYVG 127
+V+ A V G Y Q S DS+ S +SE + S I G+ IG+ Y G
Sbjct: 20 AMVLALAATVTAGVMFYYQ----SASDSNKSQNAISE---VMSATSAINGLYIGQTSYTG 72

Query: 128 ANAVI---TKNVADNSSIVGNNK 147
N+ I T + DN NNK
Sbjct: 73 LNSNILLNTSAIPDNYKDTKNNK 95


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00667NUCEPIMERASE453e-07 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 44.8 bits (106), Expect = 3e-07
Identities = 41/214 (19%), Positives = 76/214 (35%), Gaps = 26/214 (12%)

Query: 34 SFLVLGGAGSIGQAVTKEIFKRNPKKLHVVDISENNMVELVRDIRSSFGYIDGDFQTFAL 93
+LV G AG IG V+K + + + + + ++++ V L + FQ +
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLA--QPGFQFHKI 59

Query: 94 DIGSLEYDAFIKADGKFDYVLNLSALKHVR-SEKDPYTLMRMIDVNVFNTDKTIQQSIDA 152
D+ E + A G F+ V VR S ++P+ D N+ ++
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAY---ADSNLTGFLNILEGCRHN 116

Query: 153 GAKKYFCVST---------------DKAANPVNMMGASKRIMEMFLMRKSEQMAISTA-- 195
+ S+ D +PV++ A+K+ E+ S +
Sbjct: 117 KIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGL 176

Query: 196 RFANVAFSDGS---LLHGFNQRIQKRQPIVAPND 226
RF V G L F + + + + I N
Sbjct: 177 RFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNY 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00668OMS28PORIN300.014 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 29.8 bits (66), Expect = 0.014
Identities = 17/75 (22%), Positives = 34/75 (45%), Gaps = 4/75 (5%)

Query: 44 VSSVGKFVDDFERKIETYTGAAKAVATVNGTAALHAAL----YMADVQRGDLVVTQALTF 99
+ ++ K +D K+E ++ + N + + M+DV +G +V +Q T
Sbjct: 60 LDTINKVTEDVSSKLEGVRESSLELVESNDAGVVKKFVGSMSLMSDVAKGTVVASQEATI 119

Query: 100 VATCNALYHMGAEPI 114
VA C+ + GA +
Sbjct: 120 VAKCSGMVAEGANKV 134


7VIBHAR_00686VIBHAR_00713Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00686021-5.024414hypothetical protein
VIBHAR_00687125-6.921600hypothetical protein
VIBHAR_00688437-11.563887hypothetical protein
VIBHAR_00689441-13.067357UDP-N-acetylglucosamine 2-epimerase
VIBHAR_00690443-14.588177UDP-N-acetyl-D-mannosamine dehydrogenase
VIBHAR_00691748-16.512185hypothetical protein
VIBHAR_00692949-16.399225hypothetical protein
VIBHAR_00693949-16.002867hypothetical protein
VIBHAR_006941045-13.564043hypothetical protein
VIBHAR_00695943-11.689436hypothetical protein
VIBHAR_00696742-10.612906hypothetical protein
VIBHAR_00697437-8.337405hypothetical protein
VIBHAR_00698234-6.713565hypothetical protein
VIBHAR_00699233-5.755243hypothetical protein
VIBHAR_00700233-6.356568hypothetical protein
VIBHAR_00701129-5.632130glycosyl transferase family protein
VIBHAR_00702126-4.675312mannose-1-phosphate guanylyltransferase
VIBHAR_00703122-3.896081phosphomannomutase
VIBHAR_00704119-3.500656phosphomannose isomerase
VIBHAR_00705015-2.519517hypothetical protein
VIBHAR_00706-117-2.258159nucleotide sugar dehydrogenase
VIBHAR_00707120-1.915478hypothetical protein
VIBHAR_00709123-2.452848triosephosphate isomerase
VIBHAR_00710225-3.498382hypothetical protein
VIBHAR_00711123-2.302782hypothetical protein
VIBHAR_00712135-3.179766hypothetical protein
VIBHAR_00713135-3.179766hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00686OMPADOMAIN461e-08 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 46.5 bits (110), Expect = 1e-08
Identities = 44/187 (23%), Positives = 72/187 (38%), Gaps = 26/187 (13%)

Query: 9 ALSAISLGLCSFTASA----DFYAGALVSYSNAEYHHS---STSSVTEGNPFLLQAQAGY 61
A++ G + +A +Y GA + + ++YH + + + T N A GY
Sbjct: 7 AIAVALAGFATVAQAAPKDNTWYTGAKLGW--SQYHDTGFINNNGPTHENQLGAGAFGGY 64

Query: 62 FFNDYVALEARY---GTSVQRESGLAVDSLASGF---VKLNMPVSERFALYGLAGYSSVQ 115
N YV E Y G + S A G KL P+++ +Y G +
Sbjct: 65 QVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGGMVWR 124

Query: 116 IDQQN--VGSNKDQGFS--FGLGMHYALDKHNAVVFEFVDSTS-------EDQVRLSALT 164
D ++ G N D G S F G+ YA+ A E+ + + + L+
Sbjct: 125 ADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDNGMLS 184

Query: 165 LGFQHRF 171
LG +RF
Sbjct: 185 LGVSYRF 191


8VIBHAR_00826VIBHAR_00845Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00826317-0.225586acetolactate synthase 3 regulatory subunit
VIBHAR_00827317-0.290367hypothetical protein
VIBHAR_00828223-0.117242hypothetical protein
VIBHAR_008291260.455565pyruvate kinase
VIBHAR_00830-1160.890717transcriptional regulator
VIBHAR_00831-1180.914060glucosamine--fructose-6-phosphate
VIBHAR_00832-2140.238044mannitol repressor protein
VIBHAR_00833-2150.420046mannitol-1-phosphate 5-dehydrogenase
VIBHAR_00834-2150.684839PTS system, mannitol-specific II ABC component
VIBHAR_00835-120-0.184492hypothetical protein
VIBHAR_00836326-1.580849hypothetical protein
VIBHAR_00837326-1.526310hypothetical protein
VIBHAR_00838524-1.876848hypothetical protein
VIBHAR_00839222-0.827119integrase
VIBHAR_00840121-1.310982hypothetical protein
VIBHAR_00841324-1.275684integrase
VIBHAR_00842317-0.459744hypothetical protein
VIBHAR_00843115-0.145202hypothetical protein
VIBHAR_008441150.059758hypothetical protein
VIBHAR_00845219-0.309350hypothetical protein
9VIBHAR_01108VIBHAR_01120Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01108125-3.448911hypothetical protein
VIBHAR_01109028-3.743904hypothetical protein
VIBHAR_01110120-2.815123hypothetical protein
VIBHAR_01111220-3.368542hypothetical protein
VIBHAR_01112321-3.869082hypothetical protein
VIBHAR_01113218-3.573724hypothetical protein
VIBHAR_01114017-2.722233hypothetical protein
VIBHAR_01115017-2.893185type I restriction-modification system,
VIBHAR_01116119-3.367235hypothetical protein
VIBHAR_01117018-2.941079type I restriction enzyme, R subunit
VIBHAR_01118016-2.660171hypothetical protein
VIBHAR_01119015-2.414879type I restriction enzyme, R subunit
VIBHAR_01120118-3.166990hypothetical protein
10VIBHAR_01302VIBHAR_01318Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01302321-0.535472flagellin
VIBHAR_01303529-1.031298RNA-directed DNA polymerase
VIBHAR_01304430-0.703237hypothetical protein
VIBHAR_01305330-0.443566RNA-directed DNA polymerase
VIBHAR_01306233-0.883974PTS system glucose-specific transporter
VIBHAR_01307026-0.331804phosphoenolpyruvate-protein phosphotransferase
VIBHAR_01308022-0.222494phosphocarrier protein PtsH
VIBHAR_01309-120-0.469383cysteine synthase A
VIBHAR_01310-120-1.059699putative sulfate transport protein CysZ
VIBHAR_01311-122-0.758157cell division protein ZipA
VIBHAR_01312023-0.442368NAD-dependent DNA ligase LigA
VIBHAR_01313022-1.024728hypothetical protein
VIBHAR_01314218-0.321006hypothetical protein
VIBHAR_013151170.833087hypothetical protein
VIBHAR_013161181.048957hypothetical protein
VIBHAR_013171171.165689hypothetical protein
VIBHAR_013182181.063101hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01302FLAGELLIN1505e-43 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 150 bits (379), Expect = 5e-43
Identities = 79/354 (22%), Positives = 132/354 (37%), Gaps = 17/354 (4%)

Query: 5 NTNVTAMMTQRHLGHAAEQNIESQRNLTSGYRINSASDDAAGLQISNTLNVQTRGLDVAL 64
NTN +++TQ +L + + L+SG RINSA DDAAG I+N +GL A
Sbjct: 5 NTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAS 64

Query: 65 RNAHDAYSVAQTAEGALHESSDILQRLRSLGLQAANGSNDSSDRQSIQYEVVALQDELDR 124
RNA+D S+AQT EGAL+E ++ LQR+R L +QA NG+N SD +SIQ E+ +E+DR
Sbjct: 65 RNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDR 124

Query: 125 VAITTTFADKNLFDGSYGSQSFHIGANANS-ISLTLRNMRSHIPEMGGQHYVG-DAVDKD 182
V+ T F + +GAN I++ L+ + + G + G
Sbjct: 125 VSNQTQFNGVKVLSQD-NQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVG 183

Query: 183 WRVTKDNQTLKLEYQDSKGKSQTEYIRLKLGDRIEQVATYINAQQSIVSASVTENSELQF 242
+ + + T + + + +
Sbjct: 184 DLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAEN 243

Query: 243 FASTLNAPEGVELKGSLADELDVTAGGLVTMDDIDMSTAGGAQLSIGVIDAAIKYVDSHR 302
+ G+ AG + + D G +I
Sbjct: 244 NTAVDLFKTTKSTAGTAEA--KAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDG------ 295

Query: 303 SEIGGFQNRISGTMDNLNTVNRSVTESKGRIRDTDFAREATEMVRSQVLQDATT 356
++S T++ + G ++++ V + V+ T
Sbjct: 296 ------NGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFT 343



Score = 100 bits (249), Expect = 3e-25
Identities = 46/216 (21%), Positives = 87/216 (40%), Gaps = 19/216 (8%)

Query: 177 DAVDKDWRVTKDNQTLKLEYQDSKGKSQTEYIRLKLGDRIEQ-VATYINAQQSIVSASVT 235
D + +V+ K+ + + + + + +N Q + +
Sbjct: 291 TGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKN 350

Query: 236 ENSELQFFASTLNAPEGVELKGSLADELDVTAGGLVTMD------------------DID 277
E+++L + ++ + A+ AG VT+ +
Sbjct: 351 ESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDA 410

Query: 278 MSTAGGAQLSIGVIDAAIKYVDSHRSEIGGFQNRISGTMDNLNTVNRSVTESKGRIRDTD 337
+ + ID+A+ VD+ RS +G QNR + NL ++ ++ RI D D
Sbjct: 411 AAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDAD 470

Query: 338 FAREATEMVRSQVLQDATTALLAQAKQRPSSALGLL 373
+A E + M ++Q+LQ A T++LAQA Q P + L LL
Sbjct: 471 YATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01303PF05616270.008 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 27.0 bits (59), Expect = 0.008
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 7/47 (14%)

Query: 15 RRVKKNKGCAGVD----KLDIDATIF---KLRQASNGQRFARVFWME 54
RR+ + GC GVD +L D + F K S +R AR +W +
Sbjct: 136 RRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMERLARPYWEK 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01307PHPHTRNFRASE7530.0 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 753 bits (1947), Expect = 0.0
Identities = 281/571 (49%), Positives = 409/571 (71%), Gaps = 2/571 (0%)

Query: 1 MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVARFFDARNKSAAQLETIKQK 60
I+GI AS G+AI KA + E + + +I+D V E+ + A KS +L IK +
Sbjct: 4 KITGIAASSGVAIAKAFIHLEPNVDIEKTSITD--VSTEIEKLTAALEKSKEELRAIKDQ 61

Query: 61 ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKNDKMTADHAIHSVIEEQACALESLDD 120
+ G +K IF H+++L+D EL + I I+N++M A++A+ V + ES+D+
Sbjct: 62 TEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDN 121

Query: 121 EYLKERATDIRDIGSRFVKNALGINIVSLSDIDQEVILVAYDLTPSETAQINLDYVLGFA 180
EY+KERA DIRD+ R + + +G+ SL+ I +E +++A DLTPS+TAQ+N +V GFA
Sbjct: 122 EYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFA 181

Query: 181 CDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAMNNKIVINPSDAELEEAK 240
DIGGRTSH++IM+RSLE+PA+VGT ++T+++++GDM+I+D + +++NP++ E++ +
Sbjct: 182 TDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYE 241

Query: 241 AVKAAFLAEKEELAKLKDLHAETTDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFL 300
+AAF +K+E AKL + T DG VE+ NIGT KD DG++ NGGEG+GLYRTEFL
Sbjct: 242 EKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFL 301

Query: 301 FMDRDALPTEEEQYQAYKEVAEAMNGEAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAV 360
+MDRD LPTEEEQ++AYKEV + M+G+ V+IRT+DIGGDK+L Y+ LPKE+NPFLG+RA+
Sbjct: 302 YMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFRAI 361

Query: 361 RISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIRELKQAIEEYKAELRAEGHAF 420
R+ L++++I R QLR +LRAS +G L++MFPMI ++EE+R+ K ++E K +L +EG
Sbjct: 362 RLCLEKQDIFRTQLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDV 421

Query: 421 DENIEIGVMVETPAAAAIAHHLAKEVAFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA 480
++IE+G+MVE P+ A A+ AKEV FFSIGTNDL QYT+A DR NE +S+LY P PA
Sbjct: 422 SDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPA 481

Query: 481 VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNS 540
+L ++ VI A+H+EGKW GMCGE+AGDE A LLLG+GLDEFSMS SI + +
Sbjct: 482 ILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKL 541

Query: 541 NFAEVKAMAEEALSLPTAAEIEAVVEKFIAE 571
+ E+K A++AL L TA E+E +V+K +
Sbjct: 542 SKEELKPFAQKALMLDTAEEVEQLVKKTYLK 572


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01311TONBPROTEIN290.028 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 28.8 bits (64), Expect = 0.028
Identities = 16/65 (24%), Positives = 22/65 (33%), Gaps = 2/65 (3%)

Query: 124 EQVEPVLQEPAKAFAEKEEAAEVVEETLQPVFEAPLAAQAPEVEVAPVVVDPVVEEPKPE 183
V P EP +A E VVE +P +AP V P +P +
Sbjct: 48 TMVTPADLEPPQAVQPPPE--PVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKK 105

Query: 184 PEMQV 188
+ Q
Sbjct: 106 VQEQP 110


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01317IGASERPTASE320.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.0 bits (72), Expect = 0.004
Identities = 17/87 (19%), Positives = 31/87 (35%), Gaps = 3/87 (3%)

Query: 168 PPADLTAAMNAQMKAERNKRAEILEAEGVRQAEILRAEGQKQSEILKAEGEKQSAILHAE 227
+ A + + + EI + A + +E AE KQ + +
Sbjct: 995 TNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTE-TVAENSKQESKTVEK 1053

Query: 228 ARERAAQAEAKATYMVSEAIAKGDVKA 254
+ A + A+ + E AK +VKA
Sbjct: 1054 NEQDATETTAQNREVAKE--AKSNVKA 1078


11VIBHAR_01391VIBHAR_01398Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_013912190.677958bifunctional 5,10-methylene-tetrahydrofolate
VIBHAR_013933211.043097*hypothetical protein
VIBHAR_013942222.069633hypothetical protein
VIBHAR_013953222.510764hypothetical protein
VIBHAR_013962213.120181hypothetical protein
VIBHAR_013971213.137124hypothetical protein
VIBHAR_013980213.214871hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01396DHBDHDRGNASE2451e-83 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 245 bits (627), Expect = 1e-83
Identities = 110/260 (42%), Positives = 153/260 (58%), Gaps = 2/260 (0%)

Query: 14 NRSEFNGKLCLVTGAGQGIGLACAKSLLAQGARVIACDVTQLALDVFQQSLPKCQQSNTL 73
N GK+ +TGA QGIG A A++L +QGA + A D L+ SL K + +
Sbjct: 2 NAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSL-KAEARHAE 60

Query: 74 LIPFDLADSNAIQTACHNLEQDQLIPDYIVTCAGTLQLESVLDLPLEALTKTLDVNLKGT 133
P D+ DS AI +E++ D +V AG L+ + L E T VN G
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 134 MLLTQQLAKILVKHSKQGAIVAVGSNAADTPRVKMSAYCTSKAGLHMWMQCLGLELAEHG 193
++ ++K + + G+IV VGSN A PR M+AY +SKA M+ +CLGLELAE+
Sbjct: 121 FNASRSVSKYM-MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYN 179

Query: 194 IRCNIVSPGSTRTPMQEQMWNEAYGERETIQGNLASHRMGIPLNKIAETKDITNAIEFLL 253
IRCNIVSPGST T MQ +W + G + I+G+L + + GIPL K+A+ DI +A+ FL+
Sbjct: 180 IRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

Query: 254 SDKSGHITMHDLRVDGGATF 273
S ++GHITMH+L VDGGAT
Sbjct: 240 SGQAGHITMHNLCVDGGATL 259


12VIBHAR_01413VIBHAR_01419Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_014133280.125454hypothetical protein
VIBHAR_014143290.016437hypothetical protein
VIBHAR_01416430-0.064786trigger factor
VIBHAR_014173250.158541ATP-dependent Clp protease proteolytic subunit
VIBHAR_014183230.405466ATP-dependent protease ATP-binding subunit ClpX
VIBHAR_014193220.432081ATP-dependent protease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01419HTHFIS310.015 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.3 bits (71), Expect = 0.015
Identities = 39/215 (18%), Positives = 72/215 (33%), Gaps = 45/215 (20%)

Query: 261 KMPQDAREKTEQELQKLKMMSP---MSAEATV----------VRSYIDWMVSVPWNKRSK 307
MP + ++K + P MSA+ T Y+ P++
Sbjct: 56 VMPDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYL----PKPFDLTEL 111

Query: 308 VKKNLAKAEEILNEDHYGLERVKERILEYL-------AVQNRINKL---KGPILCLVGPP 357
+ + +A LE + + + + + +L ++ + G
Sbjct: 112 IG-IIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLM-ITGES 169

Query: 358 GVGKTSLGRSIAAATGRKYTRM------ALGGVRDEAEIRGHRRTYIGSLPGKLIQKMSK 411
G GK + R++ R+ A+ E+E+ GH + G+ G + +
Sbjct: 170 GTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEK---GAFTGAQTRSTGR 226

Query: 412 VGVKN--PLFLLDEIDKMSSDMRGDPSSALLEVLD 444
LF LDEI M D + + LL VL
Sbjct: 227 FEQAEGGTLF-LDEIGDMPMDAQ----TRLLRVLQ 256


13VIBHAR_01468VIBHAR_01500Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01468228-3.407528hypothetical protein
VIBHAR_01469126-2.474756hypothetical protein
VIBHAR_01470326-1.175380hypothetical protein
VIBHAR_01471325-1.741890hypothetical protein
VIBHAR_01472326-0.647825hypothetical protein
VIBHAR_01473428-1.122583hypothetical protein
VIBHAR_01474227-2.210400hypothetical protein
VIBHAR_01475225-1.970981hypothetical protein
VIBHAR_01476425-3.259387hypothetical protein
VIBHAR_01477527-4.148614hypothetical protein
VIBHAR_01478729-5.098200hypothetical protein
VIBHAR_01479626-4.158817hypothetical protein
VIBHAR_01480427-3.373376hypothetical protein
VIBHAR_01481428-4.294859hypothetical protein
VIBHAR_01482224-0.945884hypothetical protein
VIBHAR_014832240.199909hypothetical protein
VIBHAR_014841280.766816hypothetical protein
VIBHAR_014851270.591379hypothetical protein
VIBHAR_014861270.319646hypothetical protein
VIBHAR_014871250.539187hypothetical protein
VIBHAR_01488024-0.612015hypothetical protein
VIBHAR_01489228-2.824142hypothetical protein
VIBHAR_01490020-1.402464hypothetical protein
VIBHAR_01491-119-1.612404hypothetical protein
VIBHAR_01492-118-2.068615hypothetical protein
VIBHAR_01493-118-2.356875hypothetical protein
VIBHAR_01494019-2.606485hypothetical protein
VIBHAR_01495020-2.890278oxyanion-translocating ATPase
VIBHAR_01496528-4.473267hypothetical protein
VIBHAR_01497424-4.244856hypothetical protein
VIBHAR_01498320-2.162026hypothetical protein
VIBHAR_01499421-2.381466hypothetical protein
VIBHAR_01500231-1.186360hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01471SACTRNSFRASE386e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 37.6 bits (87), Expect = 6e-06
Identities = 24/116 (20%), Positives = 40/116 (34%), Gaps = 21/116 (18%)

Query: 25 EEFVIPFLSFDGQEAMRNARKRDIEQATNTD--IYTSIKAIKNDEIIGYVA----WRQGN 78
E F P+ + M D+ + + IG + W
Sbjct: 40 ERFSKPYFKQYEDDDM------DVSYVEEEGKAAFL---YYLENNCIGRIKIRSNWNGYA 90

Query: 79 YIAQLYVSSKYQKQGVGRGLI---TEMLKRSGASSIELKA-SVN--AVGFYQRLGF 128
I + V+ Y+K+GVG L+ E K + + L+ +N A FY + F
Sbjct: 91 LIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


14VIBHAR_01623VIBHAR_01653Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01623225-1.082036*integrase
VIBHAR_01624326-0.677497hypothetical protein
VIBHAR_01625327-1.489889hypothetical protein
VIBHAR_01626326-1.770101hypothetical protein
VIBHAR_01627230-4.325409integrase
VIBHAR_01628332-4.252427hypothetical protein
VIBHAR_01629331-4.237049hypothetical protein
VIBHAR_01630123-2.870154hypothetical protein
VIBHAR_01631124-4.342104acetyl-CoA acetyltransferase
VIBHAR_01632123-4.762878hypothetical protein
VIBHAR_01633222-4.857602butyrate kinase
VIBHAR_01634323-5.206384acetoacetate decarboxylase
VIBHAR_01635224-6.453124acetyl-CoA synthase
VIBHAR_01636429-6.191064hypothetical protein
VIBHAR_01637427-4.851103hypothetical protein
VIBHAR_01638326-4.575425hypothetical protein
VIBHAR_01639324-3.569221hypothetical protein
VIBHAR_01640223-3.222456hypothetical protein
VIBHAR_01641120-1.024469hypothetical protein
VIBHAR_01642119-1.506069hypothetical protein
VIBHAR_01643020-0.822918hypothetical protein
VIBHAR_01644121-1.148270hypothetical protein
VIBHAR_01645-119-1.203689hypothetical protein
VIBHAR_01646119-1.225000hypothetical protein
VIBHAR_01647-127-6.361671hypothetical protein
VIBHAR_01648-129-7.124018hypothetical protein
VIBHAR_01649028-7.404863hypothetical protein
VIBHAR_01650128-7.164838hypothetical protein
VIBHAR_01651229-7.689341hypothetical protein
VIBHAR_01652229-7.586449hypothetical protein
VIBHAR_01653120-3.913519hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01633ACETATEKNASE1772e-54 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 177 bits (451), Expect = 2e-54
Identities = 81/357 (22%), Positives = 147/357 (41%), Gaps = 39/357 (10%)

Query: 5 MKILVINPGGMSTKIAVFN------------NGFLLFNKSISHSMA--ELECFETIFEQK 50
MKILVIN G S K + + + ++H+ +++ + + + K
Sbjct: 1 MKILVINCGSSSLKYQLIESKDGNVLAKGLAERIGINDSLLTHNANGEKIKIKKDMKDHK 60

Query: 51 DFRKNLILKALKDEGIELDEIDQFVGRGGMVRNLQSGVYRINKDYLNDAAIGLNGQ---- 106
D K ++ + + + ++ + G R + G Y + + D +
Sbjct: 61 DAIKLVLDALVNSDYGVIKDMSEIDAVGH--RVVHGGEYFTSSVLITDDVLKAITDCIEL 118

Query: 107 ----HASNLGGILADELAKKTHSN-KDAYTVDPVMVDEYEDIARFSGM----ADISRVRS 157
+ +N+ GI + + + D D A + ++R
Sbjct: 119 APLHNPANIEGI---KACTQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRK 175

Query: 158 --FHALNQKAVARRYSKEHDKPYDKVNLIVAHLGSGVSVGAHKLGRIIDVNSALGG-DGP 214
FH + K V++R ++ +KP + + +I HLG+G S+ A K G+ ID + +G
Sbjct: 176 YGFHGTSHKYVSQRAAEILNKPIESLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGL 235

Query: 215 MSPERAGGVPPLALIDMCFSGEYTYKEVYKKLIGEGGVYSYLG-TKDMLEVEKRH-NEGD 272
R+G + P + + + +EV L + GVY G + D ++E GD
Sbjct: 236 AMGTRSGSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGD 295

Query: 273 EKASQVLEAMAYQVAKDIGASAAVLEGNVDAIILTGSIAYCEPMI-EWIAQRTQFVG 328
++A L AY+V K IG+ AA + G VD I+ T I P I E+I +F+G
Sbjct: 296 KRAQLALNVFAYRVKKTIGSYAAAM-GGVDVIVFTAGIGENGPEIREFILDGLEFLG 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01636FRAGILYSIN310.011 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 30.8 bits (69), Expect = 0.011
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 7/66 (10%)

Query: 258 NLITQYRAEGVNDMEFLEESVFAHEMGHLLGFNH-----DWMIS--SGAVQELLINNGHA 310
N + + A G++ + + V AHE+GH+LG H D M + +G + L N
Sbjct: 333 NTASDFEASGMSTTQLMYPGVMAHELGHILGAEHTDNSKDLMYATFTGYLSHLSEKNMDI 392

Query: 311 FSKKIG 316
+K +G
Sbjct: 393 IAKNLG 398


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01652PF00577473e-07 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 47.2 bits (112), Expect = 3e-07
Identities = 63/444 (14%), Positives = 136/444 (30%), Gaps = 90/444 (20%)

Query: 265 TVDVVRDGRTIYTQSFLKGQHGISYKHLPRGNYNVEL---VIRADGYPEERITRRINNNV 321
V + ++G IY + G I+ + + +L + ADG +I ++V
Sbjct: 310 QVTIKQNGYDIYNSTVPPGPFTIN--DIYAAGNSGDLQVTIKEADG--STQIFTVPYSSV 365

Query: 322 AQTSHKGY-DYNVTF-RESTYDSGDEGNNHLEEQLVYGDVSFTKSLF--------HDSVM 371
+G+ Y++T + ++ E + L++G + +++ + +
Sbjct: 366 PLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHG-LPAGWTIYGGTQLADRYRAFN 424

Query: 372 VGVNSQSDGEDFGFGVLANYVSDWLDVGAYYNDIDDGRFFNG---------SLSVIGFNV 422
G+ G L+ +D+ + + D +G SL+ G N+
Sbjct: 425 FGIGKNMG----ALGALS------VDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI 474

Query: 423 D---------------------YQDYDGSDKHSVTEISPLLRAVYGPE--SFDQTTLSYS 459
Y+ + V ++ P Y + L+ +
Sbjct: 475 QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVT 534

Query: 460 LPILD-SNISFYGSRVRYDGIGNSSGFESQNLALNYNTTIFRNMQLNLGFSRTLNDNANQ 518
+ S + GS Y G N + NT F ++ L +S T N
Sbjct: 535 QQLGRTSTLYLSGSHQTYWGTSNV----DEQFQAGLNTA-FEDINWTLSYSLTKNAWQKG 589

Query: 519 STDVDDIYSASITIPLGDNHT------------TYSSGLDSSSRTGQRLVNSLIYDKDKI 566
D + + ++ IP +YS D + R +D
Sbjct: 590 R---DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED-- 644

Query: 567 ELIDGVDTSGNASLNNYIDGRQSEVSLNGRINMSNDKFNSSAYANVS-NSSNTNLSFNAE 625
+ + S Y G G ++ +A S + L +
Sbjct: 645 ---NNLSYSVQTG---YAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVS 698

Query: 626 STSIITKDNIYQTKENSESYVIVE 649
+ + + + +++ V+V+
Sbjct: 699 GGVLAHANGVTLGQPLNDTVVLVK 722


15VIBHAR_01664VIBHAR_01691Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01664330-3.445480hypothetical protein
VIBHAR_01665228-4.697063hypothetical protein
VIBHAR_01666226-4.403993hypothetical protein
VIBHAR_01667226-3.768913hypothetical protein
VIBHAR_01668327-3.515062hypothetical protein
VIBHAR_01669627-0.042706hypothetical protein
VIBHAR_016706260.952577hypothetical protein
VIBHAR_016717291.551783hypothetical protein
VIBHAR_016727291.472019hypothetical protein
VIBHAR_016737281.174045hypothetical protein
VIBHAR_016746271.310715hypothetical protein
VIBHAR_01675327-2.874387hypothetical protein
VIBHAR_01676427-2.860131hypothetical protein
VIBHAR_01677328-3.105843hypothetical protein
VIBHAR_01678328-3.947922hypothetical protein
VIBHAR_01679129-4.437001hypothetical protein
VIBHAR_01680034-5.839820hypothetical protein
VIBHAR_01681130-3.940506hypothetical protein
VIBHAR_01682030-3.385653hypothetical protein
VIBHAR_01683232-4.064389hypothetical protein
VIBHAR_01684227-4.123000hypothetical protein
VIBHAR_01685126-4.206391hypothetical protein
VIBHAR_01686226-4.169070hypothetical protein
VIBHAR_01687226-4.187085hypothetical protein
VIBHAR_01688425-4.389516hypothetical protein
VIBHAR_01689323-3.819428hypothetical protein
VIBHAR_01690229-3.162625hypothetical protein
VIBHAR_01691330-3.907263hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01674UREASE310.039 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 31.2 bits (71), Expect = 0.039
Identities = 42/153 (27%), Positives = 57/153 (37%), Gaps = 51/153 (33%)

Query: 1437 GFRASQDFGAIPKAIDGDALIVAE-----------TLNG---VEQ------------YHK 1470
+ +D+G P AID L VA+ TLN VE YH
Sbjct: 217 SLKLHEDWGTTPAAID-CCLSVADEYDVQVMIHTDTLNESGFVEDTIAAIKGRTIHAYHT 275

Query: 1471 IGGGQGH---IYRIRSE-GIRGFSLNQNIEHNLNDLKVHLDL-----DLDESINPYIDRA 1521
G G GH I RI + + S N + +N L HLD+ L +I I A
Sbjct: 276 EGAGGGHAPDIIRICGQPNVIPSSTNPTRPYTVNTLAEHLDMLMVCHHLSPTIPEDIAFA 335

Query: 1522 DLMEEAHIELETVNRERHSRVKYMGPLNILNDM 1554
E+ I ET+ E +IL+D+
Sbjct: 336 ----ESRIRKETIAAE-----------DILHDI 353


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01680PF07675280.028 Cleaved Adhesin
		>PF07675#Cleaved Adhesin

Length = 1358

Score = 28.1 bits (62), Expect = 0.028
Identities = 25/87 (28%), Positives = 39/87 (44%), Gaps = 6/87 (6%)

Query: 33 DGVDISFRKTEQSEGKNIFSLYK--QKENNGTSYTFFPAKEGVEKGDWAY-VNHSSTRLD 89
DG D+ + S +++Y+ K G + T F ++GV G+ Y V T
Sbjct: 520 DGTDMEV--EDDSPASYTYTVYRDGTKIKEGLTATTF-EEDGVAAGNHEYCVEVKYTAGV 576

Query: 90 AIDVCKNYTVGGKTWFLPSSGIITSAI 116
+ VCK+ TV G F P + SA+
Sbjct: 577 SPKVCKDVTVEGSNEFAPVQNLTGSAV 603


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01689GPOSANCHOR397e-05 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 39.3 bits (91), Expect = 7e-05
Identities = 61/371 (16%), Positives = 128/371 (34%), Gaps = 24/371 (6%)

Query: 289 HQQLVSNKDAEDALMQYAMMLGSRINQENQALEQEEVQLTLQKEKVRSAKKACDDTLDKQ 348
+ AL + L ++ + L + + L+ + K++ + D
Sbjct: 70 KLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKAL 129

Query: 349 KTLQGEINALSRRQKKLADAVQKTTLIINQYDESETIESIVSGHREHLADKEQALALKQD 408
+ A S + K L L + D + +E ++ A + A K
Sbjct: 130 EGAMNFSTADSAKIKTLEAEKAA--LAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 187

Query: 409 AEKRQAELEALKQEVGKLEEMEARYLTSVNAQEFKLFNQLPESVWMKLAAVNLEVAESNP 468
E RQAELE + ++ + ++ A++ L + + A+N A+S
Sbjct: 188 LEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAK 247

Query: 469 TRPLTLSEQQAIESFTDLLEEKEQHYTLWGLELEPIQLDNRESD--QIRLSKVQETLGRK 526
+ L + +L + E + I+ E + + ++
Sbjct: 248 IKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVL 307

Query: 527 RKRMLDLNAMDSQSR------------FERVKDISTLEVEIKRTQNEINLLLAYTNDS-- 572
L SR E IS E + + +++
Sbjct: 308 NANRQSLRRDLDASREAKKQLEAEHQKLEEQNKIS--EASRQSLRRDLDASREAKKQLEA 365

Query: 573 -HQLQETNKELADAEQ--VRDSLEKQVDAQKDLWEQEKHSYDAQTDKLTKKKQQLEKLTR 629
HQ E ++++A + +R L+ +A+K + E+ +++ L K ++LE+ +
Sbjct: 366 EHQKLEEQNKISEASRQSLRRDLDASREAKKQV-EKALEEANSKLAALEKLNKELEESKK 424

Query: 630 YSVRVKAELQA 640
+ + KAELQA
Sbjct: 425 LTEKEKAELQA 435



Score = 36.2 bits (83), Expect = 7e-04
Identities = 31/298 (10%), Positives = 86/298 (28%), Gaps = 7/298 (2%)

Query: 505 QLDNRESDQIRLSKVQETLGRKRKRMLDLNAMDSQSRFERVKDISTLEVEIKRTQNEINL 564
D + + + + + K + +I
Sbjct: 86 HNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKT 145

Query: 565 LLAYTNDSHQLQETNKELADAEQVRDSLEKQVDAQKDLWEQEKHSYDAQTDKLTKKKQQL 624
L + L +L A + + A+ E EK + +A+ +L K +
Sbjct: 146 L---EAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGA 202

Query: 625 EKLTRYSVRVKAELQAHHLPVSDFSVLPDQTVDLSEETLHKRIESLNIKRQAKTDITERL 684
+ L+A ++ ++ ++ + + K + R
Sbjct: 203 MNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQ 262

Query: 685 QDFVREQVLENDDKLMLSHLDDELLEHAFHQLQTRYQGLDEEFDIL---RKHFIEHKHSV 741
+ + + + + + LE L+ L+ + +L R+ +
Sbjct: 263 AE-LEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDAS 321

Query: 742 HAIINELKNNREMVRQFEQSINRAFEGLVINDLSEVAAKIKVEARFDDLVNEIEFFDT 799
+L+ + + + + + + L + + AK ++EA L + + +
Sbjct: 322 REAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEA 379



Score = 34.3 bits (78), Expect = 0.003
Identities = 37/317 (11%), Positives = 85/317 (26%), Gaps = 24/317 (7%)

Query: 310 GSRINQENQALEQEEVQLTLQKEKVRSAKKACDDTLDKQKTLQGEINALSRRQKKLADAV 369
++ + E E L L+ + KA D D+ L E++ + +K ++
Sbjct: 52 LEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDE---LTEELSNAKEKLRKNDKSL 108

Query: 370 QKTTLIINQYDESETIESIVSGHREHLADKEQALALKQDAEKRQAELEALKQEVGKLEEM 429
+ I + + + + E A+ + LEA K
Sbjct: 109 SEKASKIQELEARK---------ADLEKALEGAMNFSTADSAKIKTLEAEKAA-----LA 154

Query: 430 EARYLTSVNAQEFKLFNQLPESVWMKLAAVNLEVAESNPTRPLTLSEQQAIESFTDLLEE 489
+ + F+ + L A + L + +
Sbjct: 155 ARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIK 214

Query: 490 KEQHYTLWGLELEPIQLDNRESDQIRLSKVQETLGRKRKRMLDLNAMDSQSRFERVKDIS 549
+ + E + + L Q+ E+ + +
Sbjct: 215 TLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL--EARQAELEKALEGA 272

Query: 550 TLEVEIKRTQNEINLLLAYTNDSHQLQETNKELADAEQVRDSLEKQVDAQKDLWEQEKHS 609
+ + L + + + R SL + +DA + + K
Sbjct: 273 MNFSTADSAKIK-TLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASR----EAKKQ 327

Query: 610 YDAQTDKLTKKKQQLEK 626
+A+ KL ++ + E
Sbjct: 328 LEAEHQKLEEQNKISEA 344


16VIBHAR_01721VIBHAR_01738Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01721222-0.259456hypothetical protein
VIBHAR_017221210.464194transcriptional regulator
VIBHAR_017230211.455576Type III secretory pathway, component EscU
VIBHAR_017242191.229471Type III secretory pathway, component EscT
VIBHAR_017252202.883471Type III secretory pathway, component EscS
VIBHAR_017262182.372695type III secretion system protein
VIBHAR_017273162.186376type III secretion system protein
VIBHAR_017284172.622109hypothetical protein
VIBHAR_017292202.659617hypothetical protein
VIBHAR_017302202.666826type III secretion system ATPase
VIBHAR_01731-1170.968563hypothetical protein
VIBHAR_01732-1171.506817hypothetical protein
VIBHAR_01733-1181.867379hypothetical protein
VIBHAR_017340160.947975hypothetical protein
VIBHAR_017350151.058930hypothetical protein
VIBHAR_017360151.247111Type III secretory pathway, component EscV
VIBHAR_017371130.857365hypothetical protein
VIBHAR_017382120.565709hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01723TYPE3IMSPROT415e-148 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 415 bits (1069), Expect = e-148
Identities = 212/346 (61%), Positives = 279/346 (80%)

Query: 1 MSGEKTELPTPKKLRDARQKGQVAKSQEVVSSALILALIAVLFAFADYYMSHISALLLLP 60
MSGEKTE PTPKK+RDAR+KGQVAKS+EVVS+ALI+AL A+L +DYY H S L+L+P
Sbjct: 1 MSGEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIP 60

Query: 61 SELAYQGFQDALLDVAIAIAKEMVYLLAPILIVAALIAIMSNMGQFGLLFSGESVKPDIK 120
+E +Y F AL V + E YL P+L VAAL+AI S++ Q+G L SGE++KPDIK
Sbjct: 61 AEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIK 120

Query: 121 KINPVEGAKRIFSLKSIIEFIKSILKVSLLSCIIWVTLRGNLNTLLQIPTCGLECVPTIT 180
KINP+EGAKRIFS+KS++EF+KSILKV LLS +IW+ ++GNL TLLQ+PTCG+EC+ +
Sbjct: 121 KINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLL 180

Query: 181 GVLVKQLMIISSVGFIVIAAADYAYQKFDHTKQLKMTKDEVKREYKEMEGSPEIKSKRRQ 240
G +++QLM+I +VGF+VI+ ADYA++ + + K+LKM+KDE+KREYKEMEGSPEIKSKRRQ
Sbjct: 181 GQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQ 240

Query: 241 LHQELQASNQRDNVKRSNVLVTNPTHIAIGLYYKKGETPLPVITLKETDAMAKRMIAIAH 300
HQE+Q+ N R+NVKRS+V+V NPTHIAIG+ YK+GETPLP++T K TDA + + IA
Sbjct: 241 FHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAE 300

Query: 301 EEGIPVMQKVPLARALYADGQLNQYIPGDLIEATAEILRWVASLEQ 346
EEG+P++Q++PLARALY D ++ YIP + IEATAE+LRW+
Sbjct: 301 EEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNI 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01724TYPE3IMRPROT1573e-49 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 157 bits (398), Expect = 3e-49
Identities = 52/249 (20%), Positives = 110/249 (44%), Gaps = 5/249 (2%)

Query: 10 LFLYSLTLPRLMACFIFLPILNKQTLGGTLVRNGVLCSLALFIFPMINQQDLPAETDGIW 69
L LY L R++A PIL+++++ V+ G+ + I P + D+P +
Sbjct: 13 LNLYFWPLLRVLALISTAPILSERSVPKR-VKLGLAMMITFAIAPSLPANDVPVFSFF-- 69

Query: 70 LMVILGKEVLLGMLIGFVAAIPFWAIEAAGFLVDNQRGAAMASMFNPTLGSQSTPTAVLL 129
+ + +++L+G+ +GF F A+ AG ++ Q G + A+ +P A ++
Sbjct: 70 ALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIM 129

Query: 130 TQTLITLFFSGGGFVAFIYALFNSYSSWPVISFFPTVTEEWVRFFYAQFEQLMWLGVLMS 189
+ LF + G + I L +++ + P+ + G++++
Sbjct: 130 DMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPL--NSNAFLALTKAGSLIFLNGLMLA 187

Query: 190 APLVLAMFLAEFGLALISRFAPQLNVFSLAMPIKSAIASVLLIVYLALMMDHFQALFSDI 249
PL+ + L L++R APQL++F + P+ + L+ + L+ + LFS+I
Sbjct: 188 LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEI 247

Query: 250 VLFREQLNT 258
+ +
Sbjct: 248 FNLLADIIS 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01725TYPE3IMQPROT635e-17 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 62.9 bits (153), Expect = 5e-17
Identities = 32/73 (43%), Positives = 47/73 (64%)

Query: 5 EIIHFTTQALTLVLFLSLPPILVAALVGTLVSLVQALTQVQEQTLGFVVKLIAVIITLFV 64
+++ +AL LVL LS P +VA ++G LV L Q +TQ+QEQTL F +KL+ V + LF+
Sbjct: 3 DLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFL 62

Query: 65 TTQWLGAELHSFA 77
+ W G L S+
Sbjct: 63 LSGWYGEVLLSYG 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01726TYPE3IMPPROT2513e-87 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 251 bits (642), Expect = 3e-87
Identities = 97/217 (44%), Positives = 143/217 (65%), Gaps = 7/217 (3%)

Query: 6 DELNLIVTLALLALIPFIAMMATSFVKLAVVFSLLRNALGVQQIPPNMALYGLAIILSIF 65
++++LI LA L+PFI T FVK ++VF ++RNALG+QQIP NM L G+A++LS+F
Sbjct: 3 NDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLSMF 62

Query: 66 IMAPVGFETYDYVTQHEISLEDSESIEGLIESGLQPYREFLKKHIRETESVFFTDAARTL 125
+M P+ + Y Y +++ D S+ ++ GL YR++L K+ FF +A
Sbjct: 63 VMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLIKYSDRELVQFFENAQLKR 122

Query: 126 WP-------QKYVDRLESDSLLLLLPAFTVSELTRAFEIGFLLYLPFIAIDLIVSNILLA 178
++ D +E S+ LLPA+ +SE+ AF+IGF LYLPF+ +DL+VS++LLA
Sbjct: 123 QYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVLLA 182

Query: 179 MGMMMVSPMTISLPFKLLLFVLLDGWTKLTHGLVLSY 215
+GMMM+SP+TIS P KL+LFV LDGWT L+ GL+L Y
Sbjct: 183 LGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQY 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01727TYPE3OMOPROT849e-21 Type III secretion system outer membrane O protein ...
		>TYPE3OMOPROT#Type III secretion system outer membrane O protein

family signature.
Length = 303

Score = 84.3 bits (208), Expect = 9e-21
Identities = 42/165 (25%), Positives = 73/165 (44%), Gaps = 7/165 (4%)

Query: 154 DTLPQAPSYL-----SQNIALPFWLSFGKTRLALSQFEQLELGDVVFFDDCYIAQHQVLL 208
+ LP+ P+ L F + T+ +L ++ +GDV+ +
Sbjct: 132 EHLPELPAVGGGRPKMLRWPLRFVIGSSDTQRSL--LGRIGIGDVLLIRTSRAEVYCYAK 189

Query: 209 QVSNHNLWRCQLDNTTLHIQEKETNMNDINSSEALTDHQQLPIELTFDVGQQTITLEQLN 268
++ + N + TL IQ E N ++E L QLP++L F + ++ +TL +L
Sbjct: 190 KLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYRKNVTLAELE 249

Query: 269 ALQPGFTFELNQPISNPVTMRANGKIIGECELVNINERLGVRVLE 313
A+ L V + ANG ++G ELV +N+ LGV + E
Sbjct: 250 AMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHE 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_0172960KDINNERMP270.044 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 26.8 bits (59), Expect = 0.044
Identities = 13/43 (30%), Positives = 25/43 (58%)

Query: 60 KQKTVVLKELEILRQEIALLREREADLKQRAAEEKKALEQENQ 102
K + + ++ +L+ +I +RER D KQR ++E AL + +
Sbjct: 374 KAQYTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEK 416


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01731PF07201295e-102 Hypersensitivity response secretion protein HrpJ
		>PF07201#Hypersensitivity response secretion protein HrpJ

Length = 293

Score = 295 bits (757), Expect = e-102
Identities = 96/292 (32%), Positives = 163/292 (55%), Gaps = 5/292 (1%)

Query: 1 MSTINSQILTGNKFDAPIRSNLESSRTGASVNGNYRGETVRVHNAT-QSLFDAMEELTAL 59
M+T+++ + GN R + SS+ G +RGE+V++ + T QS+ D EE+T +
Sbjct: 1 MTTLHN-LSYGNTPLHNERPEIASSQIVNQTLGQFRGESVQIVSGTLQSIADMAEEVTFV 59

Query: 60 GSEKAEKDLTKRKVKDGSIRVNEAHELVSDYLRKVPDLEKNQKIKDLATKMASGNLSTIA 119
SE+ E L KRK+ D RV++ E V+ YL KVP+LE+ Q + +L + +++ +++
Sbjct: 60 FSERKELSLDKRKLSDSQARVSDVEEQVNQYLSKVPELEQKQNVSELLSLLSNSPNISLS 119

Query: 120 QLQAYLNGFSEEKSHQYLALQAVKKFLGANPESKNLLALIDQAILTFEQNPDSWAQIDTE 179
QL+AYL G SEE S Q+ L ++ L PE +L L++QA+++ + +
Sbjct: 120 QLKAYLEGKSEEPSEQFKMLCGLRDALKGRPELAHLSHLVEQALVSMAEEQGETIVLGAR 179

Query: 180 IRVSSFADEYSQEQGFSSLHQLRGFYRDTVHSYQGLGSAYKDVVERFGAKEVSTAVDFML 239
I ++ + S G + L LR YRD V YQG+ + + D+ +RF ++ + + F+
Sbjct: 180 ITPEAYRESQS---GVNPLQPLRDTYRDAVMGYQGIYAIWSDLQKRFPNGDIDSVILFLQ 236

Query: 240 QGMSADLSVQGSNIDSVKLQLLMSDMQKLKTLNTLQDQVSNLYQMFKPQQAN 291
+ +SADL Q S KL +++SD+QKLK ++ DQV +Q F + N
Sbjct: 237 KALSADLQSQQSGSGREKLGIVISDLQKLKEFGSVSDQVKGFWQFFSEGKTN 288


17VIBHAR_01774VIBHAR_01780Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01774122-3.468650tryptophanyl-tRNA synthetase II
VIBHAR_01775229-4.858484hypothetical protein
VIBHAR_01776227-5.081505hypothetical protein
VIBHAR_01777430-7.672101hypothetical protein
VIBHAR_01778529-7.367047site-specific DNA-methyltransferase
VIBHAR_01779428-7.061190hypothetical protein
VIBHAR_01780326-5.800170hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01776RTXTOXIND346e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 34.4 bits (79), Expect = 6e-04
Identities = 20/168 (11%), Positives = 59/168 (35%), Gaps = 17/168 (10%)

Query: 162 WGELAEVIFADEFALYDFSLQA--EQLASTPAQDYAQAYEDLLQTWQGREAALGLESHQA 219
+ L+ I ++ + + ++ ++ TWQ ++ +
Sbjct: 153 YQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQK-----EL 207

Query: 220 KYERAVALIPDGITESEREALIPDGITESEREALIQAFKQNYLSEQESEEIVAREQQVAE 279
++ A + + +S + + +++ ++ +E + E
Sbjct: 208 NLDKKRAER-LTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHA---VLEQENKYVE 263

Query: 280 QKQQVRDYQSELQQLESRLASQRATSHASWSDSEWQRYYQQEVADFRR 327
++R Y+S+L+Q+ES + S + + ++ E+ D R
Sbjct: 264 AVNELRVYKSQLEQIESEILSAKEEY------QLVTQLFKNEILDKLR 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01779TYPE4SSCAGA320.009 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 32.0 bits (72), Expect = 0.009
Identities = 32/114 (28%), Positives = 57/114 (50%), Gaps = 8/114 (7%)

Query: 121 LATLNPTEYQEIVKKIDNYYALSNQAISDTQQQVLSALEQALDKV---FKKYPGDTLVQD 177
L+ + + QE+ +KIDN NQA+S+ + LEQ +DK+ K P + V+
Sbjct: 953 LSKVGLSRNQELAQKIDNL----NQAVSEAKAGFFGNLEQTIDKLKDSTKHNPMNLWVES 1008

Query: 178 TEKALAIL-AQLESKTRNSVVTLNDELIDLSSQQPTKQELKQLNLPIDQLIEDE 230
+K A L A+L++ NS + +N + + + + L Q N +L+ D+
Sbjct: 1009 AKKVPASLSAKLDNYATNSHIRINSNIKNGAINEKATGMLTQKNPEWLKLVNDK 1062


18VIBHAR_01938VIBHAR_01953Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01938223-1.337193hypothetical protein
VIBHAR_01939224-1.348512hypothetical protein
VIBHAR_01940223-2.131453hypothetical protein
VIBHAR_01941223-1.735062hypothetical protein
VIBHAR_01942429-3.640314hypothetical protein
VIBHAR_01943326-3.302115hypothetical protein
VIBHAR_01944326-3.628802hypothetical protein
VIBHAR_01945324-2.932812hypothetical protein
VIBHAR_01946020-1.169289hypothetical protein
VIBHAR_01947019-1.226060hypothetical protein
VIBHAR_01948-121-1.478807integrase
VIBHAR_01949019-1.451864hypothetical protein
VIBHAR_01950018-1.377745hypothetical protein
VIBHAR_01951018-1.410346hypothetical protein
VIBHAR_01952-121-1.841147hypothetical protein
VIBHAR_01953226-3.034124hypothetical protein
19VIBHAR_01962VIBHAR_01993Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01962022-3.585510hypothetical protein
VIBHAR_01963022-3.579847hypothetical protein
VIBHAR_01964025-4.723168hypothetical protein
VIBHAR_01965126-4.991471hypothetical protein
VIBHAR_01966536-7.464785hypothetical protein
VIBHAR_01967333-7.327900hypothetical protein
VIBHAR_01968428-4.630260hypothetical protein
VIBHAR_01969329-4.971416hypothetical protein
VIBHAR_01970027-3.438737hypothetical protein
VIBHAR_01971225-2.068572hypothetical protein
VIBHAR_01972023-1.463470hypothetical protein
VIBHAR_019730221.719245hypothetical protein
VIBHAR_019740242.559104hypothetical protein
VIBHAR_019750243.487232hypothetical protein
VIBHAR_019760224.110104hypothetical protein
VIBHAR_019770214.313062hypothetical protein
VIBHAR_019781204.167871hypothetical protein
VIBHAR_019792193.868816hypothetical protein
VIBHAR_019802172.947202hypothetical protein
VIBHAR_019810151.767087hypothetical protein
VIBHAR_01982117-0.102374hypothetical protein
VIBHAR_01983118-0.958925hypothetical protein
VIBHAR_01984023-1.811401hypothetical protein
VIBHAR_01985023-1.406380hypothetical protein
VIBHAR_01986023-0.564732hypothetical protein
VIBHAR_01987023-1.179000hypothetical protein
VIBHAR_01988226-1.678401hypothetical protein
VIBHAR_01989226-2.062039hypothetical protein
VIBHAR_01990226-2.253361hypothetical protein
VIBHAR_01991324-1.848142hypothetical protein
VIBHAR_01992324-1.560263hypothetical protein
VIBHAR_01993226-1.614634hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01964FRAGILYSIN290.016 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 29.3 bits (65), Expect = 0.016
Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 10/60 (16%)

Query: 141 GTAHVNFSRSFAV--LSSDAEVH--VLEHELGHLAWAWHNDMPE----YWLKGQL--LSE 190
+ +VN + F +S+ ++ V+ HELGH+ A H D + G L LSE
Sbjct: 328 NSYNVNTASDFEASGMSTTQLMYPGVMAHELGHILGAEHTDNSKDLMYATFTGYLSHLSE 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01981IGASERPTASE330.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.7 bits (74), Expect = 0.004
Identities = 39/219 (17%), Positives = 76/219 (34%), Gaps = 20/219 (9%)

Query: 223 REVREKWQSEAESTRQM--FAGKAAEYMGVDIKTILSTEAQVYEGQAAVDVGFANEVVNG 280
REV ++ +S ++ Q A +E T E +A V+ E
Sbjct: 1066 REVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQE---- 1121

Query: 281 LDAVQIMTEQFKKQQTTFDMGAAMTVQAEQQPVATGEQGNPQQAAAPATPQTPAEEQQPT 340
++ ++ KQ+ + + E P ++ Q T Q PA+E
Sbjct: 1122 --VPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQ-PAKETSSN 1178

Query: 341 PEQPQAS----------AHNDESTDPATKERERCMGIIGLEEAKGREALAQQLASNPKIS 390
EQP N E+T PAT + + + R ++ + + N + +
Sbjct: 1179 VEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSV-RSVPHNVEPA 1237

Query: 391 VDEAKVLLASVPVSATAQNESALMALASEHGEPLGDDVG 429
+ T+ N +A+++ A + + +VG
Sbjct: 1238 TTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVG 1276


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01992BCTERIALGSPC270.039 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 26.9 bits (59), Expect = 0.039
Identities = 20/58 (34%), Positives = 26/58 (44%), Gaps = 7/58 (12%)

Query: 84 REIGFWLDDGTLFGVISEPNRTLNYKAAGGHCIQPFTLNL-------SALPSDSITVI 134
R+ L+D TLFGV E N+ A+ + P TLNL S SI +I
Sbjct: 56 RQQPVTLNDFTLFGVSPEKNKAGALDASQMSNLPPSTLNLSLTGVMAGDDDSRSIAII 113


20VIBHAR_02004VIBHAR_02024Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02004226-1.063759hypothetical protein
VIBHAR_020051280.192559hypothetical protein
VIBHAR_02006128-0.027543hypothetical protein
VIBHAR_02007125-0.195844hypothetical protein
VIBHAR_02008224-0.663271hypothetical protein
VIBHAR_02009225-1.325893hypothetical protein
VIBHAR_02010225-2.251819hypothetical protein
VIBHAR_02011226-2.491880hypothetical protein
VIBHAR_02012325-2.344577RNA-directed DNA polymerase
VIBHAR_02013126-0.964093hypothetical protein
VIBHAR_02014022-2.483821hypothetical protein
VIBHAR_02015021-1.594054hypothetical protein
VIBHAR_02016021-1.479601hypothetical protein
VIBHAR_02017-120-1.636841hypothetical protein
VIBHAR_02018-121-1.754453hypothetical protein
VIBHAR_02019-123-3.082722hypothetical protein
VIBHAR_02020022-0.769785hypothetical protein
VIBHAR_02021022-1.470794hypothetical protein
VIBHAR_02022022-1.515865hypothetical protein
VIBHAR_02023119-1.565568hypothetical protein
VIBHAR_02024219-2.171232hypothetical protein
21VIBHAR_02052VIBHAR_02067Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02052220-1.801700hypothetical protein
VIBHAR_02053025-4.755747hypothetical protein
VIBHAR_02054320-2.928727hypothetical protein
VIBHAR_020555170.017341hypothetical protein
VIBHAR_02056316-0.116366hypothetical protein
VIBHAR_02057416-0.351458hypothetical protein
VIBHAR_02058-1120.585042hypothetical protein
VIBHAR_02059-1120.654229hypothetical protein
VIBHAR_020600120.810896hypothetical protein
VIBHAR_02061-3100.121751hypothetical protein
VIBHAR_02062-312-0.011784hypothetical protein
VIBHAR_02063-214-0.660432hypothetical protein
VIBHAR_02064020-2.804792hypothetical protein
VIBHAR_02065222-3.492372hypothetical protein
VIBHAR_02066226-3.715615hypothetical protein
VIBHAR_02067224-3.418884hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02060OMPADOMAIN1011e-27 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 101 bits (252), Expect = 1e-27
Identities = 43/124 (34%), Positives = 65/124 (52%), Gaps = 11/124 (8%)

Query: 107 LRMENGIGFSSSSYQLDSSIHNTLRGVARILVEY--PDTSLVIDGYTDSTGSDSYNQTLS 164
+++ + F+ + L L + L D S+V+ GYTD GSD+YNQ LS
Sbjct: 215 FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS 274

Query: 165 ERRAESVRQYLVSQKVAPGRAIARGYGERNPICSNQTSDGR---------ACNRRVEIQI 215
ERRA+SV YL+S+ + + ARG GE NP+ N + + A +RRVEI++
Sbjct: 275 ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 216 LPLK 219
+K
Sbjct: 335 KGIK 338


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02067SALSPVBPROT512e-09 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 50.5 bits (120), Expect = 2e-09
Identities = 48/184 (26%), Positives = 79/184 (42%), Gaps = 42/184 (22%)

Query: 59 KDLEALEDFSVSGYQSANDYLR-------------------ATDTSTWGQYGKTTKKFIK 99
K ++AL +S GY N YLR +T+ + ++ +I
Sbjct: 393 KQIQALRYYSAQGYSVINKYLRGDDYPETQAKETLLSRDYLSTNEPSDEEFKNAMSVYIN 452

Query: 100 QIRGAMSKLPE------YKGITYRGAWVKESLLNKLQVGDVVVEPAFTSSSAIPEVAKRF 153
I +S LPE Y+G+ + + L +G+++++ AF S+S P+ A
Sbjct: 453 DIAEGLSSLPETDHRVVYRGLKLDKPALSDVLKEYTTIGNIIIDKAFMSTS--PDKAW-- 508

Query: 154 AITRPNSPQPLQRALYEIQIKS--NGRALGGLSEFGGEAEVLLPPNTYYKVTKIELNRGS 211
+ + I ++ GR LG ++ F GEAE+L PPNT K+ I +N GS
Sbjct: 509 ----------INDTILNIYLEKGHKGRILGDVAHFKGEAEMLFPPNTKLKIESI-VNCGS 557

Query: 212 AMLA 215
A
Sbjct: 558 QDFA 561


22VIBHAR_02080VIBHAR_02091Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02080219-0.714435hypothetical protein
VIBHAR_02081120-1.22080450S ribosomal protein L35
VIBHAR_02082119-1.47984750S ribosomal protein L20
VIBHAR_02083023-1.618964hypothetical protein
VIBHAR_02084030-4.677839hypothetical protein
VIBHAR_02085234-4.553727hypothetical protein
VIBHAR_02086326-3.857653hypothetical protein
VIBHAR_02087219-1.714528hypothetical protein
VIBHAR_02088430-0.037459hypothetical protein
VIBHAR_020893230.508405hypothetical protein
VIBHAR_020902210.406446hypothetical protein
VIBHAR_020912200.366097hypothetical protein
23VIBHAR_02140VIBHAR_02154Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02140221-0.236816hypothetical protein
VIBHAR_02141121-0.534829hypothetical protein
VIBHAR_02142119-0.460858hypothetical protein
VIBHAR_02143020-1.114114glutaredoxin
VIBHAR_02144021-1.148461hypothetical protein
VIBHAR_02145022-1.398172putative SAM-dependent methyltransferase
VIBHAR_02146427-1.798965hypothetical protein
VIBHAR_02147427-0.911451hypothetical protein
VIBHAR_02148427-2.423759hypothetical protein
VIBHAR_02149328-3.673146hypothetical protein
VIBHAR_02150228-3.923995hypothetical protein
VIBHAR_02151127-3.684717hypothetical protein
VIBHAR_02152028-3.983904hypothetical protein
VIBHAR_02153028-4.104891hypothetical protein
VIBHAR_02154027-3.202724hypothetical protein
24VIBHAR_02189VIBHAR_02198Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02189315-0.723445hypothetical protein
VIBHAR_02190315-0.640591hypothetical protein
VIBHAR_02192220-1.145113hypothetical protein
VIBHAR_02191118-1.341012hypothetical protein
VIBHAR_02193215-1.192004hypothetical protein
VIBHAR_02194219-0.942127hypothetical protein
VIBHAR_02195016-0.501247chitinase
VIBHAR_02196017-0.294996hypothetical protein
VIBHAR_02197118-0.818684hypothetical protein
VIBHAR_02198216-0.079220hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02191SACTRNSFRASE388e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.0 bits (88), Expect = 8e-06
Identities = 14/47 (29%), Positives = 21/47 (44%), Gaps = 5/47 (10%)

Query: 135 RGKGIGKMLLQHAI-----KHLGAVKVDVNEQNPQAVGFYQRMGFKV 176
R KG+G LL AI H + ++ + N A FY + F +
Sbjct: 102 RKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02194MICOLLPTASE310.030 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 30.8 bits (69), Expect = 0.030
Identities = 13/42 (30%), Positives = 18/42 (42%)

Query: 689 SENNKPVAHAGEDQSVLSGATITLSADKSTDADGDKLTYVWE 730
N +P A D SV+ I +S D DG+ Y W+
Sbjct: 770 HVNKEPKAVIKSDSSVIVEEEINFDGTESKDEDGEIKAYEWD 811


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02195MICOLLPTASE343e-04 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 33.9 bits (77), Expect = 3e-04
Identities = 22/101 (21%), Positives = 38/101 (37%), Gaps = 14/101 (13%)

Query: 27 NQPPIAVATSNLYELTGAGSISLSGAQSRDPNGDLLTFEWKQLSPSSPLADIASPTTEET 86
N+ P AV S+ + I+ G +S+D +G++ +EW D
Sbjct: 772 NKEPKAVIKSD-SSVIVEEEINFDGTESKDEDGEIKAYEW----------DFGDGEKSNE 820

Query: 87 TVRFAEIAADTTYRFLLTVKD---GSLFDTSEVVVVQKAKV 124
+ Y LTV D G ++ ++ VV+ V
Sbjct: 821 AKATHKYNKTGEYEVKLTVTDNNGGINTESKKIKVVEDKPV 861


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02197TYPE3IMSPROT310.009 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 31.3 bits (71), Expect = 0.009
Identities = 25/180 (13%), Positives = 67/180 (37%), Gaps = 19/180 (10%)

Query: 90 ELTTVFILASLTAAAVSY--LIVHHLIVRSVNNVLQKANKPLAESLAKYDVLEKLSLLIP 147
E+ + ++ +L+A + H + + +++ P +++L+ ++ + L
Sbjct: 28 EVVSTALIVALSAMLMGLSDYYFEHFS-KLMLIPAEQSYLPFSQALSYV--VDNVLLEFF 84

Query: 148 IMVLDVFIPLAVGHYDWLAAIFDRILSISILVIFVWMIFALLDAMNDIATFKGITKKMPI 207
+ + A I S + F+ A+ + I +G + I
Sbjct: 85 YLCFPLLT----------VAALMAIASHVVQYGFLISGEAIKPDIKKINPIEGAKRIFSI 134

Query: 208 KSFVQLIK--LFTFFVGVVVSMSILANESPVIFLSGLGVATGF--VMLVFKDTILGFVAG 263
KS V+ +K L + +++ + I N ++ L G+ + + + ++ G
Sbjct: 135 KSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQILRQLMVICTVG 194


25VIBHAR_02315VIBHAR_02325Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02315217-0.948230hypothetical protein
VIBHAR_02316217-1.126827hypothetical protein
VIBHAR_02317526-8.548382hypothetical protein
VIBHAR_02318528-8.668647hypothetical protein
VIBHAR_02319632-11.061299hypothetical protein
VIBHAR_02320532-10.495938hypothetical protein
VIBHAR_02321532-10.472236hypothetical protein
VIBHAR_02322531-9.227714hypothetical protein
VIBHAR_02323126-3.255821hypothetical protein
VIBHAR_02324126-3.409694hypothetical protein
VIBHAR_02325124-3.568891hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02316PF05616300.021 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 29.7 bits (66), Expect = 0.021
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 1/47 (2%)

Query: 98 PTHDPDDPTGPIEDPSVLPDDSTNTFNKPDVEDEPDVEEPDTDESTD 144
P P P E+P+ P + N +P+ E +PD+ PD + TD
Sbjct: 323 PNAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDL-NPDANPDTD 368


26VIBHAR_02565VIBHAR_02580Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02565329-2.609001hypothetical protein
VIBHAR_02566430-2.262799hypothetical protein
VIBHAR_02568530-2.125025hypothetical protein
VIBHAR_02567225-2.267578hypothetical protein
VIBHAR_02569022-0.435040peptidase E
VIBHAR_02570025-0.548372hypothetical protein
VIBHAR_02571-124-0.837192hypothetical protein
VIBHAR_02572-125-0.936291hypothetical protein
VIBHAR_02573023-2.403692hypothetical protein
VIBHAR_02574025-2.127041hypothetical protein
VIBHAR_02575226-3.395326hypothetical protein
VIBHAR_02576125-3.652377hypothetical protein
VIBHAR_02577125-3.943054hypothetical protein
VIBHAR_02578121-3.887838hypothetical protein
VIBHAR_02579116-1.222885hypothetical protein
VIBHAR_02580216-0.671559hypothetical protein
27VIBHAR_02610VIBHAR_02617Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02610329-3.431803transcriptional regulator
VIBHAR_02611333-4.289728transcriptional regulator
VIBHAR_02613334-5.083761hypothetical protein
VIBHAR_02612329-4.652637hypothetical protein
VIBHAR_02614428-4.461303hypothetical protein
VIBHAR_02615221-4.269763hypothetical protein
VIBHAR_02616121-4.253487hypothetical protein
VIBHAR_02617119-3.532693hypothetical protein
28VIBHAR_02800VIBHAR_02845Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_028000294.5534515-
VIBHAR_028010303.922302hypothetical protein
VIBHAR_028020334.597342hypothetical protein
VIBHAR_028031334.474134hypothetical protein
VIBHAR_028040324.527602hypothetical protein
VIBHAR_028050313.574774hypothetical protein
VIBHAR_02806327-1.254399hypothetical protein
VIBHAR_02807427-1.189749hypothetical protein
VIBHAR_02808327-0.771938hypothetical protein
VIBHAR_02809327-0.952941hypothetical protein
VIBHAR_02810227-1.173155hypothetical protein
VIBHAR_02811126-0.805570hypothetical protein
VIBHAR_02812124-0.981306hypothetical protein
VIBHAR_02813123-0.615543hypothetical protein
VIBHAR_02814224-2.639606hypothetical protein
VIBHAR_02815023-2.493702hypothetical protein
VIBHAR_02816224-2.233944hypothetical protein
VIBHAR_02817324-2.444941hypothetical protein
VIBHAR_02818534-4.228538hypothetical protein
VIBHAR_02819536-4.853979hypothetical protein
VIBHAR_02820335-3.262864hypothetical protein
VIBHAR_02821337-3.558644hypothetical protein
VIBHAR_02822035-4.388263hypothetical protein
VIBHAR_02823137-5.313852hypothetical protein
VIBHAR_02824023-2.489697hypothetical protein
VIBHAR_02825021-1.551812hypothetical protein
VIBHAR_02826-1170.063221hypothetical protein
VIBHAR_028270160.485821hypothetical protein
VIBHAR_028290201.646748*hypothetical protein
VIBHAR_028301192.938568hypothetical protein
VIBHAR_028314202.972292hypothetical protein
VIBHAR_028323203.140105hypothetical protein
VIBHAR_028333212.888912hypothetical protein
VIBHAR_028343232.890487hypothetical protein
VIBHAR_028353222.478658hypothetical protein
VIBHAR_028362210.874549hypothetical protein
VIBHAR_02837-117-0.103796hypothetical protein
VIBHAR_02838118-0.794295hypothetical protein
VIBHAR_02839223-2.285785hypothetical protein
VIBHAR_02840221-2.104317hypothetical protein
VIBHAR_02841120-1.513578phospholipase A
VIBHAR_02842-1140.360401hypothetical protein
VIBHAR_028430170.681862acetyltransferase
VIBHAR_028440180.900070methyltransferase
VIBHAR_028452191.086812hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02805INTIMIN310.041 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 31.2 bits (70), Expect = 0.041
Identities = 13/31 (41%), Positives = 20/31 (64%)

Query: 267 GETLTDIAQQQGIALDTLQALNPHYSTSPLE 297
GET+ D+++ Q I L T+ +LN H +S E
Sbjct: 70 GETVADLSKSQDINLSTIWSLNKHLYSSESE 100


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02810DHBDHDRGNASE717e-17 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 71.2 bits (174), Expect = 7e-17
Identities = 54/194 (27%), Positives = 81/194 (41%), Gaps = 17/194 (8%)

Query: 22 ETVLITGASRGIGLELTRQFLALGYSVISTYRGQPSMQLKSMLVNSSLTLHE----LEAT 77
+ ITGA++GIG + R + G + + P K + + H +
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAA-VDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 78 DETSIANLASKLSN--VQLDILINNAGVI---GSDEQSMEAIDPKEWLNTFAVNSIAPLM 132
D +I + +++ +DIL+N AGV+ S E EW TF+VNS
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDE-----EWEATFSVNSTGVFN 122

Query: 133 VSRALLGLLETSANPRIITVSSQMGALNRESYGMYAYRSSKAAVNKVMQVLALELKPKGI 192
SR++ + + I+TV S M AY SSKAA + L LEL I
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSN--PAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 193 VVCPIHPGWVKTDM 206
+ PG +TDM
Sbjct: 181 RCNIVSPGSTETDM 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02825DNABINDNGFIS435e-09 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 43.4 bits (102), Expect = 5e-09
Identities = 18/33 (54%), Positives = 24/33 (72%)

Query: 48 LYSELMIHTRGNQTKAAKILGINRGNFSAKLKQ 80
L +M +TRGNQT+AA ++GINRG KLK+
Sbjct: 62 LLDMVMQYTRGNQTRAALMMGINRGTLRKKLKK 94


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02831SYCECHAPRONE260.050 Gram-negative bacterial type III secretion SycE cha...
		>SYCECHAPRONE#Gram-negative bacterial type III secretion SycE

chaperone signature.
Length = 130

Score = 25.8 bits (56), Expect = 0.050
Identities = 24/111 (21%), Positives = 48/111 (43%), Gaps = 4/111 (3%)

Query: 4 TFDHLVKHFFEKNGLENPTAQDGIYSLLIDQKYQLHFT-HQNEEWMVLYAELGEANPISE 62
+F+ + F++ L P + + + + + + H T H + ++ + N E
Sbjct: 3 SFEQAITQLFQQLSLSIPDTIEPVIGVKVGE-FACHITEHPVGQILMFTLPSLDNNDEKE 61

Query: 63 QLAKSSIFGEYWPAQIVSHQENSA--VLWSQQPMAMLNVAALEAWTECFIQ 111
L +IF + I+S E VLW++QP+ L+ +L E +Q
Sbjct: 62 TLLSHNIFSQDILKPILSWDEVGGHPVLWNRQPLNSLDNNSLYTQLEMLVQ 112


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02832BACSURFANTGN1564e-49 Yersinia/Haemophilus virulence surface antigen sign...
		>BACSURFANTGN#Yersinia/Haemophilus virulence surface antigen

signature.
Length = 322

Score = 156 bits (396), Expect = 4e-49
Identities = 81/210 (38%), Positives = 110/210 (52%), Gaps = 11/210 (5%)

Query: 2 KVSQVTGSIKNAILKDTNTSGGCCEALSAHWMKARAEGSNLGEQLFQSGTASDKGKLNKE 61
K +Q G+ + I+K ++T+ G CEAL AHW+++ A+G +L +QL+ G KGK +
Sbjct: 116 KFAQTKGAFLHKIIKHSDTASGVCEALCAHWIRSHAQGQSLFDQLYVGGR---KGKFQID 172

Query: 62 TMQSVAQLQTDGM-VGYDQEAITEGWLRSNNIESPYRELGALSR---VHGQTSRGRNGAA 117
T+ S+ QLQ DG DQ+ +T W + N I E L R V G T G
Sbjct: 173 TLYSIKQLQIDGCKADVDQDEVTLDWFKKNGISERMIERHCLLRPVDVTGTTES--EGLD 230

Query: 118 ELAAKIVDNGPKTSLLKKIGLEGPSHAHAVAAAV-DGEKVTFFDPNFGEFSFPSKEDFTS 176
+L I+D KKI L G AHA+AA V + VTFFDPNFGEF F KE F
Sbjct: 231 QLLNAILDTHGIGYGYKKIHLSGQMSAHAIAAYVNEKSGVTFFDPNFGEFHFSDKEKFRK 290

Query: 177 WFVQDFWHKSGYDLPKFGLSGEFSIVNLEA 206
WF FW S Y P G+ F ++ ++
Sbjct: 291 WFTNSFWGNSMYHYPL-GVGQRFRVLTFDS 319


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02838RTXTOXIND582e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 58.3 bits (141), Expect = 2e-11
Identities = 36/193 (18%), Positives = 75/193 (38%), Gaps = 14/193 (7%)

Query: 40 GTIEKQAVAVGNIVPA-HSVSIKSQIDGIVGEMYVKVGEKVKQGQALIKVRPNPTPQALT 98
G +E A A G + + S IK + IV E+ VK GE V++G L+K+
Sbjct: 78 GQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTA-------L 130

Query: 99 DASTDLMRSEADLESAKQKLANLESLVKQDIIPNNYDEYVSARSAVKSAQANVLQKRQNL 158
A D +++++ L A+ + + L I N + + + +
Sbjct: 131 GAEADTLKTQSSLLQARLEQTRYQILS--RSIELNKLPELKLPDEPYFQNVSEEEVLRLT 188

Query: 159 ELIRSGEASIGDARLTSTIYAPIDGTVLNRKVEVGEPIISTESSQAATEMMSLADMNSLI 218
LI+ ++ + + ++ + I+ + + E L D +SL+
Sbjct: 189 SLIKEQFSTWQNQKYQKE----LNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLL 244

Query: 219 FKGSVSEHDAAQL 231
K ++++H +
Sbjct: 245 HKQAIAKHAVLEQ 257



Score = 50.2 bits (120), Expect = 6e-09
Identities = 43/213 (20%), Positives = 78/213 (36%), Gaps = 29/213 (13%)

Query: 95 QALTDASTDLMRSEADLESAKQKLANLESLVKQDIIPNNYDEYVSARSAVKSAQANVLQK 154
L + L + E+++ SAK++ + L K +I ++ N+
Sbjct: 266 NELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEI-----------LDKLRQTTDNIGLL 314

Query: 155 RQNLELIRSGEASIGDARLTSTIYAPIDGTVLNRKV-EVGEPIISTESSQAATEMMSLA- 212
L A + + S I AP+ V KV G + A +M +
Sbjct: 315 TLEL-------AKNEERQQASVIRAPVSVKVQQLKVHTEGGVV------TTAETLMVIVP 361

Query: 213 DMNSLIFKGSVSEHDAAQLSPGMPVLLTVAPYPDMEISGVLTKVAIQSENLNSPEGNATA 272
+ ++L V D ++ G ++ V +P ++ KV + L++ E
Sbjct: 362 EDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNIN--LDAIEDQRLG 419

Query: 273 KSFDNGFEVEVGELKIP-KEILLRSGFSSTAQI 304
F+ +E L K I L SG + TA+I
Sbjct: 420 LVFNVIISIEENCLSTGNKNIPLSSGMAVTAEI 452


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02841PHPHLIPASEA11108e-31 Bacterial phospholipase A1 protein signature.
		>PHPHLIPASEA1#Bacterial phospholipase A1 protein signature.

Length = 289

Score = 110 bits (276), Expect = 8e-31
Identities = 66/238 (27%), Positives = 113/238 (47%), Gaps = 21/238 (8%)

Query: 55 SAHEDNYLLPFYTESKVNQARFTPTNPNAHDAKDVLVQFQISMKYKLL--VFGDDG-LFV 111
++ NYL+ T S +N+ + + KD V+FQ+S+ + L + G + L
Sbjct: 53 YPYDTNYLIYTQT-SDLNKEAIASYDWAENARKDE-VKFQLSLAFPLWRGILGPNSVLGA 110

Query: 112 AYTQRSNWEAYDD--SAYFRDDQYNPAIFY---------RWNLNPWRISLGFEHQSNGAG 160
+YTQ+S W+ + S+ FR+ Y P +F W L + +G+ H SNG
Sbjct: 111 SYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLR--DVEMGYNHDSNGRS 168

Query: 161 GSDEVSWNRVYLDNKWRFESGFVRFKTWFDVGSNHYNPDIVDFLGYGELEAAWISENDHK 220
SWNR+Y + V K W+ VG+ NPDI ++GY +L+ + D
Sbjct: 169 DPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLKIGY-HLGDAV 227

Query: 221 LNLTVSNLFTEGWEKGFYRASWNFPVYQGLRGYMKAETGYGLTISNYNFDESAFGVGF 278
L+ + G G ++P+ + +R Y + +GYG ++ +YNF+++ GVG
Sbjct: 228 LSAKGQYNWNTG--YGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQTRVGVGV 283


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02843SACTRNSFRASE376e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.8 bits (85), Expect = 6e-06
Identities = 17/82 (20%), Positives = 36/82 (43%), Gaps = 5/82 (6%)

Query: 15 CFGAFTESNQQIAFARLITDKATFAYLADVFVLPEFRGQGISKWLVSEIVSHPDLQGLRR 74
F + E N I ++ ++ +A + D+ V ++R +G+ L+ + +
Sbjct: 67 AFLYYLE-NNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCG 125

Query: 75 VMLATRD----AHGLYEQYGFK 92
+ML T+D A Y ++ F
Sbjct: 126 LMLETQDINISACHFYAKHHFI 147


29VIBHAR_02906VIBHAR_02916Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_029062241.0033733-oxoacyl-(acyl carrier protein) synthase II
VIBHAR_029073230.654697acyl carrier protein
VIBHAR_029081200.1180653-ketoacyl-ACP reductase
VIBHAR_02909016-0.386826malonyl CoA-acyl carrier protein transacylase
VIBHAR_02910-215-1.6940253-oxoacyl-ACP synthase
VIBHAR_02911018-2.213858putative glycerol-3-phosphate acyltransferase
VIBHAR_02912119-3.04146250S ribosomal protein L32
VIBHAR_02913216-0.486457hypothetical protein
VIBHAR_02914214-0.375876Maf-like protein
VIBHAR_029151150.007293hypothetical protein
VIBHAR_029172180.580625rRNA ribosomal pseudouridine synthase
VIBHAR_029162190.666425hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02908DHBDHDRGNASE1383e-42 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 138 bits (349), Expect = 3e-42
Identities = 86/251 (34%), Positives = 130/251 (51%), Gaps = 13/251 (5%)

Query: 4 LEGKIALVTGASRGIGRAIAELLVERGATVIGTATSEGGAAAISEYLGENGK---GLALN 60
+EGKIA +TGA++GIG A+A L +GA + + + L + +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 61 VTDIESIEATLKTINDEFGAIDILVNNAGITRDNLLMRMKDDEWNDIINTNLTPIYRMSK 120
V D +I+ I E G IDILVN AG+ R L+ + D+EW + N T ++ S+
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 121 AVLRGMMKKRAGRIINVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVTVNTV 180
+V + MM +R+G I+ VGS + A YA++KA + FTK + E+A + N V
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 181 APGFIETDMTKALNDDQRAATL----------SNVPAGRLGDPREIASAVVFLASPEAAY 230
+PG ETDM +L D+ A + +P +L P +IA AV+FL S +A +
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 231 ITGETLHVNGG 241
IT L V+GG
Sbjct: 246 ITMHNLCVDGG 256


30VIBHAR_02969VIBHAR_03016Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02969316-1.941834electron transport complex protein RsxE
VIBHAR_02970223-1.249200endonuclease III
VIBHAR_02971326-1.459710integrase
VIBHAR_02972326-1.259757hypothetical protein
VIBHAR_02973326-1.831977hypothetical protein
VIBHAR_02974222-0.880548hypothetical protein
VIBHAR_02975225-0.354494RNA-directed DNA polymerase
VIBHAR_02976123-1.455883hypothetical protein
VIBHAR_02977123-1.061664integrase
VIBHAR_02978221-0.474707hypothetical protein
VIBHAR_029792210.066064hypothetical protein
VIBHAR_02980426-1.299600hypothetical protein
VIBHAR_02981432-2.979042hypothetical protein
VIBHAR_02982327-2.454145hypothetical protein
VIBHAR_02983325-2.303574hypothetical protein
VIBHAR_02984223-2.635584hypothetical protein
VIBHAR_02985119-2.628291hypothetical protein
VIBHAR_02986120-2.686096hypothetical protein
VIBHAR_02987017-0.920877RNA-directed DNA polymerase
VIBHAR_02988113-0.325115hypothetical protein
VIBHAR_02989213-0.431934hypothetical protein
VIBHAR_02990214-0.834525hypothetical protein
VIBHAR_02992215-1.054298hypothetical protein
VIBHAR_02991116-0.685859ribonuclease T
VIBHAR_02993-117-0.918244hypothetical protein
VIBHAR_02994120-1.600322protein-disulfide isomerase
VIBHAR_02995226-1.950553hypothetical protein
VIBHAR_02996328-1.219326hypothetical protein
VIBHAR_02997225-0.388933hypothetical protein
VIBHAR_029981200.313375hypothetical protein
VIBHAR_029991210.525495hypothetical protein
VIBHAR_030001210.295182hypothetical protein
VIBHAR_03001-120-1.405643hypothetical protein
VIBHAR_03002021-1.663620hypothetical protein
VIBHAR_03003021-2.323325hypothetical protein
VIBHAR_03004027-4.362347hypothetical protein
VIBHAR_03005128-4.384415hypothetical protein
VIBHAR_03006128-4.681253hypothetical protein
VIBHAR_03007128-3.015997hypothetical protein
VIBHAR_03008125-2.621748hypothetical protein
VIBHAR_03009120-2.106301hypothetical protein
VIBHAR_03010119-1.036870hypothetical protein
VIBHAR_030110190.128538hypothetical protein
VIBHAR_030121200.824625hypothetical protein
VIBHAR_030135330.238309hypothetical protein
VIBHAR_030145310.375267superoxide dismutase
VIBHAR_030154240.385667hypothetical protein
VIBHAR_030163240.486186short chain dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02990NAFLGMOTY5600.0 Sodium-type flagellar protein MotY precursor signature.
		>NAFLGMOTY#Sodium-type flagellar protein MotY precursor signature.

Length = 293

Score = 560 bits (1445), Expect = 0.0
Identities = 274/293 (93%), Positives = 284/293 (96%)

Query: 1 MNKWLVTSGVLMSLLSANSFASMGKRYVATPQQSQWEMVMNTPLECQLVHPIPGFGDAVF 60
MNKWL+TSGV++SLLSANS+A MGKRYVATPQQSQWEMV+NTPLECQLVHPIP FGDAVF
Sbjct: 1 MNKWLITSGVMLSLLSANSYAVMGKRYVATPQQSQWEMVVNTPLECQLVHPIPSFGDAVF 60

Query: 61 SSHASKKINLDFELKMLRPMGETRNVSLISMPPPWRPGENADRITNLKFFKQFDGYVGGQ 120
SS ASKKINLDFELKM RPMGETRNVSLISMPPPWRPGE+ADRITNLKFFKQFDGYVGGQ
Sbjct: 61 SSRASKKINLDFELKMRRPMGETRNVSLISMPPPWRPGEHADRITNLKFFKQFDGYVGGQ 120

Query: 121 TAWGILSELEKGRYPTFSYQDWQSRDQRIEVALSSVLFQNKYNVFSSCIANLLKYSFEDI 180
TAWGILSELEKGRYPTFSYQDWQSRDQRIEVALSSVLFQ+KYN FS CIANLLKYSFEDI
Sbjct: 121 TAWGILSELEKGRYPTFSYQDWQSRDQRIEVALSSVLFQSKYNAFSDCIANLLKYSFEDI 180

Query: 181 AFTILHYERQGDQLTKASKKRLAQIADYVRHNQDIDLVLVATYTDSTDGKSTSQSLSERR 240
AFTILHYERQGDQLTKASKKRLAQIADYVRHNQDIDLVLVATYTDSTDGKS SQSLSERR
Sbjct: 181 AFTILHYERQGDQLTKASKKRLAQIADYVRHNQDIDLVLVATYTDSTDGKSESQSLSERR 240

Query: 241 AESLRSYFESLGLSGERIQVQGYGKRRPIADNGSPIGKDKNRRVVISLGRTQV 293
AESLR+YFESLGL +RIQVQGYGKRRPIADNG+PIGKDKNRRVVISLGRTQV
Sbjct: 241 AESLRTYFESLGLPEDRIQVQGYGKRRPIADNGTPIGKDKNRRVVISLGRTQV 293


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02993SECBCHAPRONE300.010 Bacterial protein-transport SecB chaperone protein ...
		>SECBCHAPRONE#Bacterial protein-transport SecB chaperone protein

signature.
Length = 170

Score = 30.3 bits (68), Expect = 0.010
Identities = 9/48 (18%), Positives = 19/48 (39%), Gaps = 3/48 (6%)

Query: 120 IPPLLGVFAKLKL---DRRLVACVLTFGLITPYMVLPIGFGGIFLNNI 164
+ L L R LV+ ++ G + P+ F +F++ +
Sbjct: 105 MAHCLTSQCPNMLFPYARELVSSLVNRGTFPALNLSPVNFDALFMDYL 152


31VIBHAR_03071VIBHAR_03076Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_03071228-1.971363hypothetical protein
VIBHAR_03072227-1.824124hypothetical protein
VIBHAR_03073225-1.743876hypothetical protein
VIBHAR_03074222-1.942686hypothetical protein
VIBHAR_03075220-1.837646hypothetical protein
VIBHAR_03076315-0.428677hypothetical protein
32VIBHAR_03148VIBHAR_03159Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_031482242.315053flagellar biosynthesis protein FlhB
VIBHAR_031493222.443199flagellar biosynthesis protein FliR
VIBHAR_031504212.316374flagellar biosynthesis protein FliQ
VIBHAR_031512162.386444flagellar biosynthesis protein FliP
VIBHAR_031521152.036572hypothetical protein
VIBHAR_031531152.004967flagellar motor switch protein
VIBHAR_031540162.543319flagellar motor switch protein FliM
VIBHAR_031551172.363828flagellar basal body-associated protein FliL
VIBHAR_031560132.626307hypothetical protein
VIBHAR_03157-1173.060994hypothetical protein
VIBHAR_031580183.298516flagellar biosynthesis chaperone
VIBHAR_03159-1173.472771flagellum-specific ATP synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03148TYPE3IMSPROT359e-125 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 359 bits (922), Expect = e-125
Identities = 111/354 (31%), Positives = 189/354 (53%), Gaps = 14/354 (3%)

Query: 8 ERTEEATPRRLQQAREKGQVARSKELASASVLIVGAIALMWFGESLARSLFSIMSRLFDL 67
E+TE+ TP++++ AR+KGQVA+SKE+ S ++++ + LM L+ F S+L +
Sbjct: 4 EKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLM----GLSDYYFEHFSKLMLI 59

Query: 68 TRDEIFDTAKLFDIAFGAMTDLLFPLFLI--LITLFVAAMIGAAG---VGGISFSAEAAM 122
++ + F A + D + F L VAA++ A G S EA
Sbjct: 60 PAEQSYLP---FSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIK 116

Query: 123 PKLSKMNPLSGLKRMVGMQSWVELIKSILKVALVTGMAIYLIQASQADLIQLSMDVYPQN 182
P + K+NP+ G KR+ ++S VE +KSILKV L++ + +I+ + L+QL +
Sbjct: 117 PDIKKINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQL-PTCGIEC 175

Query: 183 IFHAL-DILLNFILLISCSLLIVVAIDIPFQIWQHADQLKMTKQEVKDEYKETEGKPEVK 241
I L IL +++ + +++ D F+ +Q+ +LKM+K E+K EYKE EG PE+K
Sbjct: 176 ITPLLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIK 235

Query: 242 GRIRMLQREAAQRRMMADVPQADVIITNPEHFSVALRYKQKTDRAPVVIAKGTDHMAMKI 301
+ R +E R M +V ++ V++ NP H ++ + YK+ P+V K TD +
Sbjct: 236 SKRRQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTV 295

Query: 302 REVAREHDITIVPAPPLARALYHTTELEQEIPDGLFTAVAQVLAFVFQLKQYRK 355
R++A E + I+ PLARALY ++ IP A A+VL ++ + ++
Sbjct: 296 RKIAEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNIEKQ 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03149TYPE3IMRPROT1277e-38 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 127 bits (321), Expect = 7e-38
Identities = 87/221 (39%), Positives = 131/221 (59%), Gaps = 2/221 (0%)

Query: 9 LDWIANYFWPYTRISAMLMVMTVTGARFVSPRIRLYLGLAITFAVAPAIPAVPQDIELLS 68
L W+ YFWP R+ A++ + R V R++L L + ITFA+AP++PA D+ + S
Sbjct: 10 LSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA--NDVPVFS 67

Query: 69 FRGFMTIAEQIVIGVAMGMVTQFMIQTFVLLGQILGMQSSLGFASMVDPANGQNTPLLGQ 128
F +QI+IG+A+G QF G+I+G+Q L FA+ VDPA+ N P+L +
Sbjct: 68 FFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLAR 127

Query: 129 LFMFLATMFFLATDGHLKMLQLVVFSFKTLPIGSGSLNAVDFREMASWLGIMFKTALSMS 188
+ LA + FL +GHL ++ L+V +F TLPIG LN+ F + ++F L ++
Sbjct: 128 IMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLA 187

Query: 189 LSGIIALLTINLSFGVMTRAAPQLNIFSLGFAFALMVGLLL 229
L I LLT+NL+ G++ R APQL+IF +GF L VG+ L
Sbjct: 188 LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISL 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03150TYPE3IMQPROT541e-13 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 54.4 bits (131), Expect = 1e-13
Identities = 26/70 (37%), Positives = 39/70 (55%)

Query: 7 VELFRDALWMVLIMVCAIIIPSLLIGLVVAIFQAATSINEQTLSFLPRLIVTLLALMLFG 66
V AL++VLI+ I + +IGL+V +FQ T + EQTL F +L+ L L L
Sbjct: 5 VFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFLLS 64

Query: 67 HWMTQMLMEY 76
W ++L+ Y
Sbjct: 65 GWYGEVLLSY 74


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03151FLGBIOSNFLIP2847e-99 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 284 bits (728), Expect = 7e-99
Identities = 113/229 (49%), Positives = 165/229 (72%), Gaps = 1/229 (0%)

Query: 60 MSVGNGGGIPAFTMTTNADGSEDYSVTLQILALMTMLGFLPAMVILMTSFTRIVVVMSIL 119
++ +P T G + +S+ +Q L +T L F+PA++++MTSFTRI++V +L
Sbjct: 15 ITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVFGLL 74

Query: 120 RQAMGLQQTPSNQVIIGIALFLTFFVMSPVLNEINDNAIQPYLNEQVTARQAFDAAQVPM 179
R A+G P NQV++G+ALFLTFF+MSPV+++I +A QP+ E+++ ++A + P+
Sbjct: 75 RNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGAQPL 134

Query: 180 KAFMLKQTRIKDLETFVNMSGE-QVTNPEDVSMAVLIPAFITSELKTAFQIGFMLFLPFL 238
+ FML+QTR DL F ++ + PE V M +L+PA++TSELKTAFQIGF +F+PFL
Sbjct: 135 REFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFL 194

Query: 239 IIDLVVASVLMAMGMMMLSPMIVSLPFKLMLFVLVDGWNLILSTLAGSF 287
IIDLV+ASVLMA+GMMM+ P ++LPFKLMLFVLVDGW L++ +LA SF
Sbjct: 195 IIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03153FLGMOTORFLIN1146e-36 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 114 bits (287), Expect = 6e-36
Identities = 63/135 (46%), Positives = 91/135 (67%), Gaps = 8/135 (5%)

Query: 3 PSDDQK--LADEWAAALGEDPSAPSIDVDEVLAAPLEELKDTSSPITDDERRKLDTIMDI 60
PSD+ L D WA AL E + + + + L D S + D +D IMDI
Sbjct: 7 PSDENTGALDDLWADALNEQKATTTKSAADAVFQQLGG-GDVSGAMQD-----IDLIMDI 60

Query: 61 PVTISMEVGRSQISIRNLLQLNQGSVVELDRLAGESLDVLVNGTLIAHGEVVVVNDKFGI 120
PV +++E+GR++++I+ LL+L QGSVV LD LAGE LD+L+NG LIA GEVVVV DK+G+
Sbjct: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120

Query: 121 RLTDVISQTERIKKL 135
R+TD+I+ +ER+++L
Sbjct: 121 RITDIITPSERMRRL 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03154FLGMOTORFLIM2482e-82 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 248 bits (634), Expect = 2e-82
Identities = 85/327 (25%), Positives = 161/327 (49%), Gaps = 9/327 (2%)

Query: 1 MTDLLSQDEIDALLHGVD--DVDDVDEPMDAGDESAVSFDFSSQDRIVRGRMPTLELINE 58
MT++LSQDEID LL + D D + +DF D+ + +M TL L++E
Sbjct: 1 MTEVLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHE 60

Query: 59 RFARHMRISLFNMLRKTAEVSINGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITME 118
FAR SL LR V + V + + E+ ++ P++L ++ PLKG A++ ++
Sbjct: 61 TFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVD 120

Query: 119 ARLVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIVFEDYKEAWSPVMGVEFEY 178
+ F +++ FGG G+ R+ T E +++ ++ + + +E+W+ V+ +
Sbjct: 121 PSITFSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRL 178

Query: 179 LDSEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAG--VQSD 236
E NP A IV P+E++V+ + +V G + +PY +EPI L + S
Sbjct: 179 GQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSV 238

Query: 237 KMETDVRWSSALREEIMDCPVNFRVNLLEKDISLRDLMELQPGDVIPIE---MPEHATMF 293
+ + ++ LR+++ ++ + +S+RD++ L+ GD+I + + + +
Sbjct: 239 RRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLS 298

Query: 294 IEDLPTYRVKMGRSEDKMAVQISEEIE 320
I + + + G K+A QI E IE
Sbjct: 299 IGNRKKFLCQPGVVGKKIAAQILERIE 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03155PilS_PF08805270.036 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 26.8 bits (59), Expect = 0.036
Identities = 10/49 (20%), Positives = 22/49 (44%), Gaps = 2/49 (4%)

Query: 13 KGKSKLLIIIIAVVVLLLGGGSAAFFLMGSDEPAQAAQAEQTQAQTAAA 61
KG + + ++++ V+++L A+ L + + EQ T A
Sbjct: 26 KGATLMEVLLVVGVIVVLAAS--AYKLYSMVQSNIQSSNEQNNVLTVIA 72


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03156FLGHOOKFLIK465e-07 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 45.6 bits (107), Expect = 5e-07
Identities = 27/117 (23%), Positives = 60/117 (51%)

Query: 555 AEKVQMMMSKNLKNLDIRLDPPELGRMQIRMTMNNDLANVHFTVANPQARDLIEQTLPRL 614
++ + + + ++ ++RL P +LG +QI + ++++ A + + R +E LP L
Sbjct: 245 SQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVL 304

Query: 615 REMLAQQGMQLADSSVQQQSSGQQQSGYANAEQNGQGSQGRGFSGQSDENFDADTNL 671
R LA+ G+QL S++ +S QQ + +Q+ + + +G+ D+ +L
Sbjct: 305 RTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSL 361


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03158FLGFLIJ407e-07 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 39.8 bits (92), Expect = 7e-07
Identities = 31/144 (21%), Positives = 75/144 (52%)

Query: 2 NNAMEFLLEQAKEREDQAVLALNKARSELDDYYRQVEQIEKYRLDYCQQLVDRGMAGLTA 61
+ A+ L + A++ + A L + R Q++ + Y+ +Y L AG+T+
Sbjct: 4 HGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAGITS 63

Query: 62 SQYGHLNRFLTQLDETLSKQKQAENHFKEQVVNCQDYWLNMRKERMSYEWMIEKREKEKQ 121
+++ + +F+ L++ +++ +Q N + ++V + W ++ +++ + E++
Sbjct: 64 NRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQSTAAL 123

Query: 122 MAEAKREQKQMDEFSTLLYSRKPQ 145
+AE + +QK+MDEF+ RKP+
Sbjct: 124 LAENRLDQKKMDEFAQRAAMRKPE 147


33VIBHAR_03331VIBHAR_03350Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_033312252.501023UDP-glucose 4-epimerase
VIBHAR_033323241.569561hypothetical protein
VIBHAR_033332252.133991hypothetical protein
VIBHAR_033342261.531743DNA-binding transcriptional repressor EbgR
VIBHAR_033353252.312145hypothetical protein
VIBHAR_033372242.719536beta-D-galactosidase, alpha subunit
VIBHAR_033381171.248359cryptic beta-D-galactosidase subunit beta
VIBHAR_033391160.918615hypothetical protein
VIBHAR_033400180.177504transcriptional regulator
VIBHAR_03341-118-0.805448hypothetical protein
VIBHAR_03342223-2.989235hypothetical protein
VIBHAR_03343325-4.680833Flp pilus assembly protein TadG
VIBHAR_03344327-5.242255hypothetical protein
VIBHAR_03345425-4.233231hypothetical protein
VIBHAR_03346325-3.917330hypothetical protein
VIBHAR_03347325-3.680678hypothetical protein
VIBHAR_03348325-2.641802Flp pilus assembly protein TadB
VIBHAR_03349226-1.393233Flp pilus assembly protein, ATPase
VIBHAR_03350226-1.572807hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03331NUCEPIMERASE1802e-56 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 180 bits (459), Expect = 2e-56
Identities = 80/345 (23%), Positives = 142/345 (41%), Gaps = 35/345 (10%)

Query: 1 MKVLVTGGMGYIGSHTCVQMIEAGMEPIIVDNLCNAKVEVL--SRIEALTGKQPAFYQGD 58
MK LVTG G+IG H +++EAG + + +DNL + L +R+E L F++ D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 59 IRDEAFWDSVFAKHDIQAVIHFAGLKAVGESVAKPLEYYDNNVNGSLVLARSMLKAGVKS 118
+ D +FA + V AV S+ P Y D+N+ G L + ++
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 119 IVFSSSATVYGDPDIVPITEDSPTGATTNPYGRSKYMVEECLSDLFNAENDWSVTLLRYF 178
++++SS++VYG +P + D + Y +K E ++ ++ T LR+F
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATK-KANELMAHTYSHLYGLPATGLRFF 179

Query: 179 NPVGAHPSGTMGEDPQGIPNNLMPFIAQVAVGRREKLSVYGNDYPTPDGTGVRDYIHVMD 238
G P G P ++ F A+ + + VY G RD+ ++ D
Sbjct: 180 TVYG----------PWGRP-DMALFKFTKAMLEGKSIDVYN------YGKMKRDFTYIDD 222

Query: 239 LADGHIAALKSVGEK---------------AGLHIYNLGTGKGSSVLEMVEAFATACGNP 283
+A+ I + A +YN+G +++ ++A A G
Sbjct: 223 IAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIE 282

Query: 284 VPYELCPRRPGDIAECWASTDKAERDLGWKATRSVAEMTADTWKW 328
+ P +PGD+ E A T +G+ +V + + W
Sbjct: 283 AKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNW 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03342OMPADOMAIN961e-25 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 96.2 bits (239), Expect = 1e-25
Identities = 36/116 (31%), Positives = 52/116 (44%), Gaps = 3/116 (2%)

Query: 107 VTDKGKLIARVHFAFDRFNLTPLARDVLSSVARQLAQT---DYQVTVEGHTDWIGTKAYN 163
T L + V F F++ L P + L + QL+ D V V G+TD IG+ AYN
Sbjct: 211 QTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYN 270

Query: 164 QTLGHNRAKSTARELHYYGVSEQKTTLKSFGESEPIATNNTSSGRSKNRRADVYIP 219
Q L RA+S L G+ K + + GES P+ N + + + D P
Sbjct: 271 QGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAP 326


34VIBHAR_03389VIBHAR_03396Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_033892250.408532hypothetical protein
VIBHAR_033902260.378258multidrug efflux pump VmrA
VIBHAR_03391537-0.115819hypothetical protein
VIBHAR_033927410.202208lipoprotein NlpI
VIBHAR_03393847-0.093320polynucleotide phosphorylase/polyadenylase
VIBHAR_p2740-0.23051430S ribosomal protein S15
VIBHAR_03394639-0.338143tRNA pseudouridine synthase B
VIBHAR_03395539-0.087269ribosome-binding factor A
VIBHAR_03396335-0.197051translation initiation factor IF-2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03396TCRTETOQM764e-16 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 75.7 bits (186), Expect = 4e-16
Identities = 69/313 (22%), Positives = 112/313 (35%), Gaps = 77/313 (24%)

Query: 400 IMGHVDHGKTSTLDYIRRTHVASGEAG------------------GITQHIGAYHVETDN 441
++ HVD GKT+ + + A E G GIT G + +N
Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67

Query: 442 GMITFLDTPGHAAFTAMRARGAQATDIVVLVVAADDGVMPQTVEAIQHAKAAGVPLIVAV 501
+ +DTPGH F A R D +L+++A DGV QT + G+P I +
Sbjct: 68 TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFI 127

Query: 502 NKIDKEDANPD----NVKNELAQYDVI-----------------PEEWG----------- 529
NKID+ + ++K +L+ VI E+W
Sbjct: 128 NKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLE 187

Query: 530 ---------------GENMFV---------HISAKQGTNIDGLLEAILLQSEVLELTAVA 565
E++ H SAK ID L+E I ++ T
Sbjct: 188 KYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVIT--NKFYSSTHRG 245

Query: 566 EGMASGVVVESRLDKGRGPVATVLVQSGTLNKGDIVL-CGQEYGRVRAMRDELGKEITEA 624
+ G V + + R +A + + SG L+ D V +E ++ M + E+ +
Sbjct: 246 QSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKI 305

Query: 625 GPSIPVEILGLSG 637
+ EI+ L
Sbjct: 306 DKAYSGEIVILQN 318


35VIBHAR_03457VIBHAR_03463Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_03457229-0.732176carbonic anhydrase
VIBHAR_034581370.741969hypothetical protein
VIBHAR_034594420.740859hypothetical protein
VIBHAR_034604410.718206hypothetical protein
VIBHAR_034614380.410249hypothetical protein
VIBHAR_034623370.435028dihydrolipoamide dehydrogenase
VIBHAR_034633340.310182dihydrolipoamide acetyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03459HTHTETR603e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 59.6 bits (144), Expect = 3e-13
Identities = 26/208 (12%), Positives = 69/208 (33%), Gaps = 20/208 (9%)

Query: 4 IAKRPRTRLSPLKRKQQLMEIALEVFARRGIGRGGHADIAEIAQVSVATVFNYFPTREDL 63
+A++ + +Q ++++AL +F+++G+ +IA+ A V+ ++ +F + DL
Sbjct: 1 MARKTKQEAQE--TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDL 58

Query: 64 VDEVLNHVVRQFSNFLSDNIDLDIHARENIANITNAMIELVSQDCH------WLKVWFEW 117
E+ + + ++ + +I ++ +++ F
Sbjct: 59 FSEIWELSESNIGELELEYQAKF--PGDPLSVLREILIHVLESTVTEERRRLLMEIIFHK 116

Query: 118 SASTRDEVWPLFVTTNRTNQLLVQNMFI----KAIERGEVCDQHEPEHLANLFHGICYSI 173
+ + R L + IE + A + G +
Sbjct: 117 CEFVGE--MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174

Query: 174 FVQANRSKSEAELTN----LVSAYLDML 197
+ +L V+ L+M
Sbjct: 175 MENWLFAPQSFDLKKEARDYVAILLEMY 202


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03463RTXTOXIND357e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 35.2 bits (81), Expect = 7e-04
Identities = 50/281 (17%), Positives = 90/281 (32%), Gaps = 28/281 (9%)

Query: 26 DKVEEEQSLITVEGDKASMEVPASQAGIVKEIKVAEGDSVTTGSLIMIFEAEAAGAPGAA 85
+ V +T G S E+ + IVKEI V EG+SV G +++ A A A
Sbjct: 81 EIVATANGKLTHSGR--SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 86 AAAPAPAAEAAPVAAPAPAAAAELKEVHVPDIGGDEV--EVTEIMVAVGDSIEEEQSLLT 143
+ A + + EL ++ + + V+E V S+ +EQ T
Sbjct: 139 TQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ-FST 197

Query: 144 VEGDKASMEVPAPFAGTLKEIKVAAGDKVTTGSLIMVFETAGSGAAAAPAAV---EAPAA 200
+ K E+ + +A ++ S + + A+
Sbjct: 198 WQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQ 257

Query: 201 PAAAPAAAADKEVNVPDIGGDEVEVTEVMV-------------------AVGDTVEEEQS 241
A + V + E E+ +
Sbjct: 258 ENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLE 317

Query: 242 LITVEGDKASMEVPAPFAGTVKEIKIAA-GDKVTTGSLIMV 281
L E + + + AP + V+++K+ G VTT +MV
Sbjct: 318 LAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMV 358


36VIBHAR_03533VIBHAR_03544Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_035332251.162820pyridoxine 5'-phosphate synthase
VIBHAR_035341221.198682recombinational DNA repair protein
VIBHAR_035351241.052417GTP-binding protein Era
VIBHAR_03536-1191.080389ribonuclease III
VIBHAR_035370190.798800signal peptidase
VIBHAR_035380151.637169GTP-binding protein LepA
VIBHAR_035392151.782731positive regulator of sigma E activity
VIBHAR_035402161.532329periplasmic negative regulator of sigmaE
VIBHAR_035413141.978997negative regulator of sigma E activity
VIBHAR_035422162.161971RNA polymerase sigma factor RpoE
VIBHAR_035433162.120225L-aspartate oxidase
VIBHAR_035442152.185695hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03538TCRTETOQM1513e-41 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 151 bits (384), Expect = 3e-41
Identities = 102/452 (22%), Positives = 179/452 (39%), Gaps = 87/452 (19%)

Query: 1 MKHIRNFSIIAHIDHGKSTLSDRLIQVCGG---LSDREMAAQVLDSMDLERERGITIKSQ 57
MK I N ++AH+D GK+TL++ L+ G L + D+ LER+RGITI++
Sbjct: 1 MK-IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTG 59

Query: 58 SVTLNYTAKDGETYQLNFIDTPGHVDFAYEVSRSLAACEGALLVVDAGQGVEAQTLANCY 117
+ + E ++N IDTPGH+DF EV RSL+ +GA+L++ A GV+AQT +
Sbjct: 60 ITSFQW-----ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFH 114

Query: 118 TAIEMDLEVVPILNKIDLPAADPERVAEEIEEIVGIDAMEATRCSAKTGIGV-------- 169
+M + + +NKID D V ++I+E + + + + + V
Sbjct: 115 ALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQ 174

Query: 170 --------DDVLENIVSAIPAPEGDPDAPLQALIID------------------------ 197
DD+LE +S + + +
Sbjct: 175 WDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVI 234

Query: 198 -SWF--------------------DNYLGVVSLVRIKNGSLKKNDKIKVMSTGQTWGVDR 236
+ F ++ +R+ +G L D +++ S + +
Sbjct: 235 TNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRI-SEKEKIKITE 293

Query: 237 LGIFTP-KQVDTDVLRTGEVGWVVCGIKDILGAPVGDTLTLAKNGS-EKPLPGFKKVKPQ 294
+ + D +GE+ ++ L + +GDT L + E PLP +
Sbjct: 294 MYTSINGELCKIDKAYSGEIV-ILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTT--- 349

Query: 295 VYAGLFPVSSDDYENFRDALGKLSLNDASL-FYEPENSAALGFGFRCGFLGMLHMEIIQE 353
+ P E DAL ++S +D L +Y + + FLG + ME+
Sbjct: 350 ----VEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIIL----SFLGKVQMEVTCA 401

Query: 354 RLEREYDLDLITTAPTVVY-EVEQTNGETMYV 384
L+ +Y +++ PTV+Y E E
Sbjct: 402 LLQEKYHVEIEIKEPTVIYMERPLKKAEYTIH 433



Score = 35.6 bits (82), Expect = 7e-04
Identities = 16/67 (23%), Positives = 27/67 (40%), Gaps = 1/67 (1%)

Query: 398 EIREPISRCNILVPADYLGNVITLCVEKRGVQVDMVYHGNQVAITYDIPMAEVVLDFFDR 457
E+ EP I P +YL T + VD N+V ++ +IP + ++
Sbjct: 534 ELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARC-IQEYRSD 592

Query: 458 LKSTSRG 464
L + G
Sbjct: 593 LTFFTNG 599


37VIBHAR_03559VIBHAR_03564Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_03559330-0.641850hypothetical protein
VIBHAR_03560747-1.266780hypothetical protein
VIBHAR_03561539-1.327084hypothetical protein
VIBHAR_035624430.094236hypothetical protein
VIBHAR_035633440.432175hypothetical protein
VIBHAR_035642400.681268fructose-bisphosphate aldolase
38VIBHAR_03635VIBHAR_03665Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_036352240.896780leucyl aminopeptidase
VIBHAR_036361210.355919DNA polymerase III subunit chi
VIBHAR_036372230.456642valyl-tRNA synthetase
VIBHAR_03638-115-1.035209hypothetical protein
VIBHAR_03639-1120.460091hypothetical protein
VIBHAR_03640-1130.389583hypothetical protein
VIBHAR_03641-118-0.231505hypothetical protein
VIBHAR_036421200.611296hypothetical protein
VIBHAR_036431200.570697hypothetical protein
VIBHAR_036442231.054586arginine deiminase
VIBHAR_036453200.906727ornithine carbamoyltransferase
VIBHAR_036463230.721262hypothetical protein
VIBHAR_036471201.509291aspartate carbamoyltransferase catalytic
VIBHAR_036481201.327280aspartate carbamoyltransferase regulatory
VIBHAR_036490181.356010hypothetical protein
VIBHAR_03650-1171.045248RNA-directed DNA polymerase
VIBHAR_036510180.0112751-acyl-sn-glycerol-3-phosphate acyltransferase
VIBHAR_036520170.313947UDP-N-acetylglucosamine
VIBHAR_03653015-0.063750hypothetical protein
VIBHAR_036542130.111281hypothetical protein
VIBHAR_036552120.360852hypothetical protein
VIBHAR_036562130.383029hypothetical protein
VIBHAR_036572130.827215hypothetical protein
VIBHAR_036580140.719522hypothetical protein
VIBHAR_03659-1120.292846hypothetical protein
VIBHAR_03660-114-0.169152hypothetical protein
VIBHAR_03661-217-0.535709hypothetical protein
VIBHAR_03662-213-0.381784hypothetical protein
VIBHAR_03663-115-0.170112hypothetical protein
VIBHAR_036640191.044743RNA polymerase factor sigma-54
VIBHAR_036652191.413853ribosome-associated protein Y
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03639SACTRNSFRASE320.002 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 31.8 bits (72), Expect = 0.002
Identities = 11/48 (22%), Positives = 20/48 (41%)

Query: 213 PQTRGHGLAKRIVALSLQLAKQFGYQQCYLETTECLREAVGLYEKLGF 260
R G+ ++ +++ AK+ + LET + A Y K F
Sbjct: 99 KDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03644ARGDEIMINASE421e-148 Bacterial arginine deiminase signature.
		>ARGDEIMINASE#Bacterial arginine deiminase signature.

Length = 409

Score = 421 bits (1084), Expect = e-148
Identities = 147/411 (35%), Positives = 226/411 (54%), Gaps = 11/411 (2%)

Query: 1 MSKLYVGSEVGQLRRVLLNRPERALTHLTPSNCHELLFDDVLAVEAAGEEHDAFARTLRE 60
++ + + SE+G+L++VLL+RP L +LTP LFDD+ +E A +EH+ FA L+
Sbjct: 5 LNPINIFSEIGRLKKVLLHRPGEELENLTPFIMKNFLFDDIPYLEVARQEHEVFASILKN 64

Query: 61 QDVEVLLLHDLLVETLAVPEA-KQWLLNTQISDFRYGPTFARD-LRQYFAEMDDEHLASI 118
VE+ + DL+ E L A + ++ I + F + L+ YF+ + +++ S
Sbjct: 65 NLVEIEYIEDLISEVLVSSVALENKFISQFILEAEIKTDFTINLLKDYFSSLTIDNMISK 124

Query: 119 LLGGLAYSELPIKSSSMLPKMKRPLDFVIEPLPNHLFTRDTSCWVYGGVSLNPMMMPARQ 178
++ G+ EL +SS+ + F+I+P+PN LFTRD + GV++N M RQ
Sbjct: 125 MISGVVTEELKNYTSSLDDLVNGANLFIIDPMPNVLFTRDPFASIGNGVTINKMFTKVRQ 184

Query: 179 RETNHLRAIYRWHPTFAGQDFINYFGDEDQHYDNANVEGGDVLVIGKGAVLIGMSERTTP 238
RET I+++HP + + + ++ A++EGGD LV+ KG ++IG+SERT
Sbjct: 185 RETIFAEYIFKYHPVYKE-NVPIWLNRWEE----ASLEGGDELVLNKGLLVIGISERTEA 239

Query: 239 QGVENLAASLFK-SGQAKEVIAIDLPKHRSCMHLDTVMTHMDVDTFSVYPEIMRKDLDTW 297
+ VE LA SLFK ++A +PK+RS MHLDTV T +D F+ + +
Sbjct: 240 KSVEKLAISLFKNKTSFDTILAFQIPKNRSYMHLDTVFTQIDYSVFTSFTSDD-MYFSIY 298

Query: 298 RLTPKGNG-EMH-VEASHNYLHAIESALGLDQLKIITTGGDSYEAEREQWNDANNVLTVK 355
LT + ++H + + LG I GGD REQWND NVL +
Sbjct: 299 VLTYNPSSSKIHIKKEKARIKDVLSFYLGRKIDIIKCAGGDLIHGAREQWNDGANVLAIA 358

Query: 356 PGVVIGYERNVYTNEKYDKAGIEVLTIPGNELGRGRGGARCMSCPIERDDI 406
PG +I Y RN TN+ +++ GI+V IP +EL RGRGG RCMS P+ R+DI
Sbjct: 359 PGEIIAYSRNHVTNKLFEENGIKVHRIPSSELSRGRGGPRCMSMPLIREDI 409


39VIBHAR_00123VIBHAR_00130N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_001232172.017552preprotein translocase subunit SecB
VIBHAR_001242182.332349NAD(P)H-dependent glycerol-3-phosphate
VIBHAR_001252232.925266serine acetyltransferase
VIBHAR_001260202.771710peptidase
VIBHAR_00127-1202.690234acetyltransferase
VIBHAR_001280252.324246TetR family transcriptional regulator
VIBHAR_001290252.655486hypothetical protein
VIBHAR_00130-1222.568055lysyl-tRNA synthetase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00123SECBCHAPRONE2105e-73 Bacterial protein-transport SecB chaperone protein ...
		>SECBCHAPRONE#Bacterial protein-transport SecB chaperone protein

signature.
Length = 170

Score = 210 bits (537), Expect = 5e-73
Identities = 79/152 (51%), Positives = 112/152 (73%), Gaps = 2/152 (1%)

Query: 9 AEAAPQDAQQNFAIQRIFLKDVSFEAPNSPVMFQKEWNPDVKLDLDTQSRELGEGVYEIV 68
A Q IQRI++KDVSFEAPN P +FQ++W P + DL T+++++G+ +YE+
Sbjct: 9 AADTQATQQPVLQIQRIYVKDVSFEAPNLPHIFQQDWEPKLSFDLSTEAKQVGDDLYEVC 68

Query: 69 LRLTV--TVKNEEETAFLCEVQQGGIFTAEKMEAGQLAHCLGAFCPNILFPYARETISSL 126
L ++V T+++ + AF+CEV+Q G+FT +E Q+AHCL + CPN+LFPYARE +SSL
Sbjct: 69 LNISVETTMESSGDVAFICEVKQAGVFTISGLEEMQMAHCLTSQCPNMLFPYARELVSSL 128

Query: 127 VVKGTFPQLNLAPVNFDALFMNYLQQQAQQGE 158
V +GTFP LNL+PVNFDALFM+YLQ+Q Q +
Sbjct: 129 VNRGTFPALNLSPVNFDALFMDYLQRQEQAEQ 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00126RTXTOXIND416e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.0 bits (96), Expect = 6e-06
Identities = 20/129 (15%), Positives = 43/129 (33%), Gaps = 17/129 (13%)

Query: 35 AASQQELKGVSSEINRQKKSLTSQEKQLNDLQKSLKNQELGISKL---EREIKQTKTDLA 91
+ E V + INR + ++ +L+D L Q + + E + + +L
Sbjct: 210 DKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELR 269

Query: 92 QADQNINKLEEKIALQEAQRQAQEEELKQLLQTYYVTERAKANGHLLNEGVEEDRISQYF 151
+ ++E +I + + Q + K + + + D I
Sbjct: 270 VYKSQLEQIESEILSAKEEYQLVTQLFKNEIL----DKLRQT----------TDNIGLLT 315

Query: 152 QHLAKARAK 160
LAK +
Sbjct: 316 LELAKNEER 324



Score = 36.7 bits (85), Expect = 1e-04
Identities = 36/232 (15%), Positives = 77/232 (33%), Gaps = 30/232 (12%)

Query: 26 SATYPLTSSAASQQELKGVSSEINRQKKSLTSQEKQLNDLQKSLKNQELGISKLEREIKQ 85
L + Q + +S I K + S + S ++ +
Sbjct: 138 KTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFST 197

Query: 86 TKTDLAQADQNINKLEEKIALQEAQRQAQEEELKQLLQTYYVTERAKANGHLLNEGVEED 145
+ Q + N++K + A+ E VE+
Sbjct: 198 WQNQKYQKELNLDKKRAERLTVLARINRYENLS----------------------RVEKS 235

Query: 146 RISQYFQHLAKARAKVIDAITQTTQELAHNKNQLELEKAQIETLLKQQSEKRSSLAS-TQ 204
R+ + L +A A+ + + N+L + K+Q+E + + + TQ
Sbjct: 236 RLDDF-SSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQ 294

Query: 205 SQRKGTLNKIQKSIKDDKRYLAELQRNETRLKAEIAKAAKRNAVPMDGIAKQ 256
+ L+K++++ + EL +NE R +A + +A P+ +Q
Sbjct: 295 LFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRA------PVSVKVQQ 340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00128HTHTETR631e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 63.5 bits (154), Expect = 1e-14
Identities = 27/114 (23%), Positives = 51/114 (44%), Gaps = 3/114 (2%)

Query: 1 MKTRDKIVYAALELFNQHGERNITTNHIADHIEISPGNLYYHFRNKQEIVREIFALYSAE 60
+TR I+ AL LF+Q G + + IA ++ G +Y+HF++K ++ EI+ L +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 61 LLERF--TPIQGSQESLTMLKSYLDSIFTLMWKYRFFYANLPEILSRDEQLHDE 112
+ E + + L++L+ L + L EI+ + E
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEE-RRRLLMEIIFHKCEFVGE 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00130UREASE300.015 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 29.7 bits (67), Expect = 0.015
Identities = 14/64 (21%), Positives = 23/64 (35%), Gaps = 2/64 (3%)

Query: 60 GPGYADGSKLFFMTSPEFHMKRLLAAGSGCIYQINKAFRNEENGRHHNPEFTMLEWYRIG 119
G G A G+ T +H+ R++ A + +N AF + N M+
Sbjct: 160 GTGPAHGTLATTCTPGPWHIARMIEAADA--FPMNLAFAGKGNASLPGALVEMVLGGATS 217

Query: 120 FDHH 123
H
Sbjct: 218 LKLH 221


40VIBHAR_00480VIBHAR_00490N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00480-2150.714035multidrug efflux pump
VIBHAR_00481-310-0.476022hypothetical protein
VIBHAR_00482-2120.433615NADH dehydrogenase
VIBHAR_00483-1160.914480ATP-dependent DNA helicase
VIBHAR_00484-2161.152939cb-type cytochrome oxidase subunit III
VIBHAR_00490-2171.243665*****hydrolase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00480ACRIFLAVINRP8270.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 827 bits (2137), Expect = 0.0
Identities = 317/1033 (30%), Positives = 531/1033 (51%), Gaps = 30/1033 (2%)

Query: 5 DVFIKRPVLAVSISFLIALLGLQAVFKMQVREYPEMTNTVVTVTTSYYGASADLIQGFIT 64
+ FI+RP+ A ++ ++ + G A+ ++ V +YP + V+V+ +Y GA A +Q +T
Sbjct: 3 NFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVT 62

Query: 65 QPLEQAVAQADNIDYMTSSSV-LGTSTITVNMKLNTDPNAALSDILAKTNSVRSQLPKEA 123
Q +EQ + DN+ YM+S+S G+ TIT+ + TDP+ A + K LP+E
Sbjct: 63 QVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEV 122

Query: 124 EDPTVTMSTGSTTAVLYIGFTSDELSSSQ--ITDYLERVINPQLFTVNGVSKVDLYGGMK 181
+ +++ S++ ++ GF SD ++Q I+DY+ + L +NGV V L+G +
Sbjct: 123 QQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA-Q 181

Query: 182 YALRVWLDPAKMGALKLTATDVMTVLNANNYQSATGQATGEFVL------YNGSADTQVS 235
YA+R+WLD + KLT DV+ L N Q A GQ G L + A T+
Sbjct: 182 YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 236 NVAELEALVVKTG-EGDVIRLGDIAKVTLEKSHDIYRANANGQEAVVAAINAAPSANPIN 294
N E + ++ +G V+RL D+A+V L + A NG+ A I A AN ++
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 295 ISADVLKLLPQLERNLPSNIKMNVMYDSTIAINESIHEVVKTILEAAVIVLVVITLFLGS 354
+ + L +L+ P +K+ YD+T + SIHEVVKT+ EA ++V +V+ LFL +
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 355 FRAVIIPIVTIPLSLIGVAMVMQAMGFSWNLMTLLAMVLAIGLVVDDAIVVLENVDRHIK 414
RA +IP + +P+ L+G ++ A G+S N +T+ MVLAIGL+VDDAIVV+ENV+R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 EGESPFRAAII-GTREIAVPVIAMTLTLGAVYAPIALMGGITGSLFKEFALTLAGAVFVS 473
E + P + A +I ++ + + L AV+ P+A GG TG+++++F++T+ A+ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 474 GIIALTLSPMMCSKMLRAN-----EEPSKFEQKVHHVLDGMTNRYAKMLTAVMAHRPVII 528
++AL L+P +C+ +L+ E F + D N Y + ++ +
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 529 AFAIIVFTTLPILFKFIPSELAPSEDKGVVVLMGTAPSNANLDYIEHTMRDVNKILSNQP 588
++ + +LF +PS P ED+GV + M P+ A + + + V
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 589 EVEYAQVFN------GVPNSNQAFGLATLKPWSER---EASQADITKRVGELVSHVPGMS 639
+ VF N +LKPW ER E S + R + +
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 640 VTAFQMPE--LPGAGSGLPIQFVITTPNSFESLFTIASDILADVSTSPLFVYS-DLDLNY 696
V F MP G +G + + ++L + +L + P + S +
Sbjct: 662 VIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLE 721

Query: 697 DSATMKISIDKDKAGAYGVTMQDIGVTLSTMMADGYVNRIDLNGRSYEVIPQVERKWRLN 756
D+A K+ +D++KA A GV++ DI T+ST + YVN GR ++ Q + K+R+
Sbjct: 722 DTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRML 781

Query: 757 PESMKNYYVRSVDGKAVPLGSLITIDVVAEPRSLPHFNQLNSATVGAVPSPGVAMGDAIN 816
PE + YVRS +G+ VP + T V L +N L S + +PG + GDA+
Sbjct: 782 PEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMA 841

Query: 817 WFEDVAQNKLPSGYNHDYMGEARQFVTEGSALYATFGLALAIIFLVLAIQFESIRDPIVI 876
E++A +KLP+G +D+ G + Q G+ A ++ ++FL LA +ES P+ +
Sbjct: 842 LMENLA-SKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 877 MVSVPLAVCGALIALAWGTASMNIYSQVGLITLVGLITKHGILICEVAKEEQLHNKLSRI 936
M+ VPL + G L+A ++Y VGL+T +GL K+ ILI E AK+ +
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 937 DAVMEAAKVRLRPILMTTAAMIAGLIPLMYATGAGAAQRFSIGIVIVAGLAIGTLFTLFV 996
+A + A ++RLRPILMT+ A I G++PL + GAG+ + ++GI ++ G+ TL +F
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 997 LPVIYSYLAEKHK 1009
+PV + + K
Sbjct: 1021 VPVFFVVIRRCFK 1033


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00481RTXTOXIND568e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 56.0 bits (135), Expect = 8e-11
Identities = 42/190 (22%), Positives = 73/190 (38%), Gaps = 21/190 (11%)

Query: 101 DSDVEKANLQSSQARLPAAKAKYKRYQGLFKKGSVSKEAYDEAEANYFSLKADIESLKAT 160
+ V K+ L+ ++ + +AK +Y+ LFK + K + N L ++ +
Sbjct: 267 ELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLR--QTTDNIGLLTLELAKNEER 324

Query: 161 IDRREIKAPFTGVVGIRNVY-LGQYLQAGT---DIVRLEDTSVMRLGFTVSQTDISRIKL 216
I+AP + V V+ G + IV +DT + V DI I +
Sbjct: 325 QQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTAL--VQNKDIGFINV 382

Query: 217 DQEVDIFVDAYPDK---PFKGSISAIEPAV-------NVQSGLIQVQADIPNSDGK---L 263
Q I V+A+P G + I V + +I ++ + ++ K L
Sbjct: 383 GQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNKNIPL 442

Query: 264 RSGMFARANI 273
SGM A I
Sbjct: 443 SSGMAVTAEI 452



Score = 41.7 bits (98), Expect = 3e-06
Identities = 14/59 (23%), Positives = 28/59 (47%)

Query: 71 TVANETSGVIDKISFESGTQVDTGQSLIHLDSDVEKANLQSSQARLPAAKAKYKRYQGL 129
+ + ++ +I + G V G L+ L + +A+ +Q+ L A+ + RYQ L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQIL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00482HTHTETR644e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 64.3 bits (156), Expect = 4e-15
Identities = 21/67 (31%), Positives = 34/67 (50%)

Query: 7 RKDKREQIVIAAERLIAESGFQGLSMQKLADEAGVAAGTIYRYFSDKEHLLKEVRLIVTT 66
++ R+ I+ A RL ++ G S+ ++A AGV G IY +F DK L E+ + +
Sbjct: 9 AQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSES 68

Query: 67 RIAEAVQ 73
I E
Sbjct: 69 NIGELEL 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00490SECA280.042 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 27.9 bits (62), Expect = 0.042
Identities = 37/183 (20%), Positives = 63/183 (34%), Gaps = 57/183 (31%)

Query: 82 GLIQTILAEQGIEIDAEQAQQA----QSFFDSARMGAFTFFPGVKEML-------TELRQ 130
GL Q + A++G++I E A Q++ F + + M E
Sbjct: 352 GLHQAVEAKEGVQIQNENQTLASITFQNY--------FRLYEKLAGMTGTADTEAFEFSS 403

Query: 131 HYKLVVI---TNGPIF--SQHPKLQATQMSEW---VDHI----------IVGGEEPEEKP 172
YKL + TN P+ + T+ + ++ I +VG E+
Sbjct: 404 IYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEK-- 461

Query: 173 AASIFHKAL-------NLVDAKPEEAIHIGDSLPADI-AGANNMGILSVWVNEAGTANPT 224
+ + L N+++AK A I A A +++ N AG T
Sbjct: 462 -SELVSNELTKAGIKHNVLNAKFHAN-------EAAIVAQAGYPAAVTIATNMAGRG--T 511

Query: 225 DIK 227
DI
Sbjct: 512 DIV 514


41VIBHAR_00594VIBHAR_00612N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00594-2152.209774D-tyrosyl-tRNA(Tyr) deacylase
VIBHAR_00595-3151.969290acetyltransferase
VIBHAR_00596-1141.396621hypothetical protein
VIBHAR_005971171.373660phosphoenolpyruvate carboxykinase
VIBHAR_005980141.179742Hsp33-like chaperonin
VIBHAR_005990141.097243heat shock protein
VIBHAR_00600-1150.751080zinc metallopeptidase
VIBHAR_00601-1160.781356general secretion pathway protein D
VIBHAR_00602-1191.082288general secretory pathway protein E
VIBHAR_00603-1190.755541general secretion pathway protein F
VIBHAR_00604-1181.251015general secretion pathway protein G
VIBHAR_00605-1191.051415general secretion pathway protein H
VIBHAR_00606-1191.680931general secretion pathway protein I
VIBHAR_00607-2172.277104general secretion pathway protein J
VIBHAR_00608-2152.077728general secretion pathway protein K
VIBHAR_006090142.105639general secretion pathway protein L
VIBHAR_00610-1171.837138general secretion pathway protein M
VIBHAR_006110172.376982general secretion pathway protein N
VIBHAR_00612-2162.6600023'(2'),5'-bisphosphate nucleotidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00594SHIGARICIN270.033 Ribosome inactivating protein family signature.
		>SHIGARICIN#Ribosome inactivating protein family signature.

Length = 289

Score = 26.7 bits (59), Expect = 0.033
Identities = 8/16 (50%), Positives = 10/16 (62%)

Query: 3 EARVVIALIQRVSEAA 18
A ++ LIQ SEAA
Sbjct: 170 AASALMVLIQSTSEAA 185


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00596IGASERPTASE404e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 40.0 bits (93), Expect = 4e-05
Identities = 65/355 (18%), Positives = 120/355 (33%), Gaps = 58/355 (16%)

Query: 398 GLNLEGDQLEVKHNNGHWGIWSGKLDASVVNASYDQVITSHAAITTQSDDGLWQLTRLFA 457
+NL V +G + ++ V +T ++ + + QL
Sbjct: 818 NVNLTESANFVLGKANLFGTIQSRGNSQVR-------LTENSHWHLTGNSDVHQLD---- 866

Query: 458 PLEQGYIEGLGQIDLSTTSQPWTLNLNADGIPLLLLHPYLPTALAVNGFSDLSLDLKGLA 517
L G+I + + ++ TL +N+ L+ NG DL
Sbjct: 867 -LANGHIHLNSADNSNNVTKYNTLTVNS---------------LSGNGSFYYLTDLSNKQ 910

Query: 518 GDQNMLAYSLSGEVEANLRDTTLKSQADDSLKAVTFSPLRLRAQRGEVKLQPVTISGKAI 577
GD+ ++ S +G + D T + L S +AQR + V++ G +
Sbjct: 911 GDKVVVTKSATGNFTLQVADKT-GEPNHNELTLFDAS----KAQRDHLN---VSLVGNTV 962

Query: 578 SG--------NLSGEFDMANNPLSGVIYQLKDTCGVIKGDVLSGEIETNECQNKPKQKEQ 629
N++G +D+ NP Q DT + + + ++ + N+ +
Sbjct: 963 DLGAWKYKLRNVNGRYDL-YNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVD 1021

Query: 630 TEPEQ--SAPEPSKVTRIAAINLEQHEEEISEEVMEEEEPTATSHSEEQLISAPEVSANN 687
P + PS+ T A N +Q + + + + E TA + E + V AN
Sbjct: 1022 EAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQN-REVAKEAKSNVKANT 1080

Query: 688 EINE---------EASPEERNTPETNHSVTEQGASAN--QDAPQETPASSPLTAK 731
+ NE E E T + Q+ P+ T SP +
Sbjct: 1081 QTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQ 1135



Score = 35.4 bits (81), Expect = 0.001
Identities = 20/112 (17%), Positives = 39/112 (34%), Gaps = 9/112 (8%)

Query: 616 ETNECQNKPKQ-KEQTEPEQSAPEPSKVTRIAAINLEQHEEEISEEVMEEEEPTATSHSE 674
ET E K+ K + E E++ P ++++ + + E E +PT
Sbjct: 1099 ETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEP 1158

Query: 675 EQLISAPEVSANNEINEEASPEERNTPETNHSVTEQGASANQDAPQETPASS 726
+ S N + P + + VTE ++ E P ++
Sbjct: 1159 Q--------SQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENT 1202


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00600BCTERIALGSPC2191e-72 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 219 bits (558), Expect = 1e-72
Identities = 80/283 (28%), Positives = 135/283 (47%), Gaps = 33/283 (11%)

Query: 30 LSLFLTCLFIAMTGWILGKVVW-LAMPQTSDVT--QWKPSAGAVASTNKKDEIDFNALQN 86
+ L L + + L + W + +P + V+ Q P A A L +
Sbjct: 14 IRRILFYLLMLLFCQQLAMIFWRIGLPDNAPVSSVQITP---AQARQQPV------TLND 64

Query: 87 ANLFGKYTEQK-PVVVEQPVVQDAPKTRLNLTLAGAVASSNAKTSLAVIANRGKQATYGI 145
LFG E+ ++ + + P + LNL+L G +A + S+A+I+ +Q + G+
Sbjct: 65 FTLFGVSPEKNKAGALDASQMSNLPPSTLNLSLTGVMAGDDDSRSIAIISKDNEQFSRGV 124

Query: 146 GEEIDGTRAELKAVLVDRVIIDNQGRDETLMLEGIEYSKLSETRPAARPQAAKADKSSAV 205
EE+ G A++ ++ DRV++ QGR E L L E ++ P A
Sbjct: 125 NEEVPGYNAKIVSIRPDRVVLQYQGRYEVLGLYSQE-----DSGSDGVPGA--------- 170

Query: 206 SEKVDQIRAEIS-KDPQSVFKYINISPVKKDGGIVGYRVSPGRDAALFNDVGLKPGDIAV 264
Q+ ++ + ++ Y++ SP+ D + GYR++PG + F VGL+ D+AV
Sbjct: 171 -----QVNEQLQQRASTTMSDYVSFSPIMNDNKLQGYRLNPGPKSDSFYRVGLQDNDMAV 225

Query: 265 QLNGIDLSDPSSSVELMQVMNDPQELNLTVERDGQQYDIYIQL 307
LNG+DL D + + M+ M D LTVERDGQ+ DIY++
Sbjct: 226 ALNGLDLRDAEQAKKAMERMADVHNFTLTVERDGQRQDIYMEF 268


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00601BCTERIALGSPD6260.0 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 626 bits (1616), Expect = 0.0
Identities = 312/619 (50%), Positives = 431/619 (69%), Gaps = 34/619 (5%)

Query: 1 MANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEKQYYSFFLNVL 60
A EFSASFKGTDIQEFIN V +NL KT+I+DPSVRG I VRSYD+LNE+QYY FFL+VL
Sbjct: 26 AAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVL 85

Query: 61 EVYGYAVVEMDNGVLKVIKAKDSKTSAIPVLGDDSAQ-GDSVITRVVAVRNVSVRELSPL 119
+VYG+AV+ M+NGVLKV+++KD+KT+A+PV D + GD V+TRVV + NV+ R+L+PL
Sbjct: 86 DVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVVPLTNVAARDLAPL 145

Query: 120 LRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEIIKRVDQAGDKEIEVVELRNASAS 179
LRQL DNAG G+VVHY+P+N++L+TGRAAV+ RL I++RVD AGD+ + V L ASA+
Sbjct: 146 LRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPLSWASAA 205

Query: 180 EMVRIVDALNKTTNQKSTPEFLEPKIVADDRTNSILISGDPKVRARLKRLIRQLDVEMAT 239
++V++V LNK T++ + P + +VAD+RTN++L+SG+P R R+ +I+QLD + AT
Sbjct: 206 DVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRIIAMIKQLDRQQAT 265

Query: 240 KGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKQAGQNGASSAQRGEVVIAAHEATNSLVL 299
+GN +V+YLKYAKA DLV+VL G+S +Q+EKQA + +A ++I AH TN+L++
Sbjct: 266 QGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKP--VAALDKNIIIKAHGQTNALIV 323

Query: 300 TAQPDIMMALQDVISQLDIRRAQVLIEALIVEMSEGDGINLGVQWGNLDTGAVIQYGNTG 359
TA PD+M L+ VI+QLDIRR QVL+EA+I E+ + DG+NLG+QW N + G + Q+ N+G
Sbjct: 324 TAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAG-MTQFTNSG 382

Query: 360 AQIGNVMVGLEEAKDTTKTESYWDEDANGGSGDWNTREYIEEGDYSTLAAALGGVNGAAM 419
I + G + +G S+LA+AL NG A
Sbjct: 383 LPISTAIAGAN------------QYNKDGTVS-------------SSLASALSSFNGIAA 417

Query: 420 SIMMGDWTALITAVSSDSNSNILSSPSITVMDNGEASFIVGEEVPVITGSTSGSNNDNPF 479
G+W L+TA+SS + ++IL++PSI +DN EA+F VG+EVPV+TGS + S DN F
Sbjct: 418 GFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTS-GDNIF 476

Query: 480 QTVDRKEVGIKLKVVPQINEGDSVQLTIEQEVSNVL----GANGAVDVRFAKRQLNTSVM 535
TV+RK VGIKLKV PQINEGDSV L IEQEVS+V + + F R +N +V+
Sbjct: 477 NTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVL 536

Query: 536 IQDGQMLVLGGLVDERALESESKVPLLGDIPVIGQLFKSTSTQTEKRNLMVFIKPTIIRD 595
+ G+ +V+GGL+D+ ++ KVPLLGDIPVIG LF+STS + KRNLM+FI+PT+IRD
Sbjct: 537 VGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRD 596

Query: 596 GMTADGITQRKYNFIRAEQ 614
+ +Y Q
Sbjct: 597 RDEYRQASSGQYTAFNDAQ 615


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00603BCTERIALGSPF5320.0 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 532 bits (1371), Expect = 0.0
Identities = 218/407 (53%), Positives = 306/407 (75%), Gaps = 2/407 (0%)

Query: 1 MAAFEYKALDAKGKQKKGTIEGDNARQVRQRLKEQGMIPVEVMEAKAKAAKSSGGSVGFK 60
MA + Y+ALDA+GK+ +GT E D+ARQ RQ L+E+G++P+ V E + KS + +
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 61 RGIK--TAELALITRQLSTLVQSGMPLEECLKAVSEQAEKPRIRSMIAAVRSKVTEGYPL 118
R I+ T++LAL+TRQL+TLV + MPLEE L AV++Q+EKP + ++AAVRSKV EG+ L
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 119 ADSLGDYPHVFDELYLSMVAAGEKSGHLDTVLERLADYVENRQKMRSKLLQAMIYPVVLV 178
AD++ +P F+ LY +MVAAGE SGHLD VL RLADY E RQ+MRS++ QAMIYP VL
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 179 VFAVGIVSLLLSSVVPKIIEPIIQMGQELPQSTQFLLSASEFVQEWGLIIFVVVVVFFYG 238
V A+ +VS+LLS VVPK++E I M Q LP ST+ L+ S+ V+ +G + + ++ F
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 239 LKLALQKPNFRLAWDQKILSLPLIGKISRGLNTSRFARTLSICTSSAIPILEGMRVAVDV 298
++ L++ R+++ +++L LPLIG+I+RGLNT+R+ARTLSI +SA+P+L+ MR++ DV
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 299 MSNRYVKQQVLVAADNVREGASLRKALDQTRLFPPMMLHMISSGEQSGELESMLTRAADN 358
MSN Y + ++ +A D VREG SL KAL+QT LFPPMM HMI+SGE+SGEL+SML RAADN
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 359 QDQNFESTVNIALGIFTPVLIALMAGLVLFIVMATLMPMLEMNNLMS 405
QD+ F S + +ALG+F P+L+ MA +VLFIV+A L P+L++N LMS
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLMS 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00604BCTERIALGSPG2168e-76 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 216 bits (551), Expect = 8e-76
Identities = 86/141 (60%), Positives = 110/141 (78%), Gaps = 4/141 (2%)

Query: 4 KRSKQQGFTLLEVMVVVVILGILASVVVPNLLGNKEKADQQKAITDIVALENALDMYKLD 63
KQ+GFTLLE+MVV+VI+G+LAS+VVPNL+GNKEKAD+QKA++DIVALENALDMYKLD
Sbjct: 3 ATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLD 62

Query: 64 NSVYPTTDQGLDALVNKPS-NPEPRNYRDGGYIKRLPSDPWSNEYQYLSPGDNGTIDIFT 122
N YPTT+QGL++LV P+ P NY GYIKRLP+DPW N+Y ++PG++G D+ +
Sbjct: 63 NHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLLS 122

Query: 123 LGADGQEGGEGPAADIGNWNM 143
G DG+ G E DI NW +
Sbjct: 123 AGPDGEMGTED---DITNWGL 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00605BCTERIALGSPH1011e-29 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 101 bits (254), Expect = 1e-29
Identities = 45/175 (25%), Positives = 73/175 (41%), Gaps = 20/175 (11%)

Query: 9 GFTLIEILLVLVLLSLTAVAVIATLPTSQKDLSKQYAQSFFQRLQLLNEEAVLSGKDFGL 68
GFTL+E++L+L+L+ ++A V+ P S+ D + Q F +L+ + + + +G+ FG+
Sbjct: 5 GFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQFFGV 64

Query: 69 RVDDVKSTYTLLSLTQDGWQPLELKQIPSDTKLEGDISVQLELGGGAWG--DDDRLFEPG 126
V D WQ L L+ D G W R+ G
Sbjct: 65 SVHP------------DRWQFLVLEARDGADPAPADDGWS----GYRWLPLRAGRVATSG 108

Query: 127 SLFDEDM-FAELEDKKKKKVKPPQVFILSSAEITPFTLSFFPEEGDAFN-DGWRI 179
S+ + A + + P V I E+TPF L+ G AFN G +
Sbjct: 109 SIAGGKLNLAFAQGEAWTPGDNPDVLIFPGGEMTPFRLTLGEAPGIAFNARGESL 163


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00606AEROLYSIN280.006 Aerolysin signature.
		>AEROLYSIN#Aerolysin signature.

Length = 493

Score = 28.5 bits (63), Expect = 0.006
Identities = 11/31 (35%), Positives = 14/31 (45%)

Query: 68 AGRTWYWKVTPVATEQPLLKAFDVSVATEKE 98
A TW + PV E P L A D+ E +
Sbjct: 93 ASNTWCYPTNPVTGEIPTLSALDIPDGDEVD 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00607BCTERIALGSPH310.003 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 31.1 bits (70), Expect = 0.003
Identities = 21/97 (21%), Positives = 38/97 (39%), Gaps = 17/97 (17%)

Query: 15 SNRGFTLIE---VLVSIAIFASMSVAAYQVVSQVQRSNVLSQER---TQRLNELQRAMVM 68
RGFTL+E +L+ + + A M + A+ R + +Q +L +Q+ +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAF----PASRDDSAAQTLARFEAQLRFVQQRGLQ 57

Query: 69 MDNDF-------RQMAMRQVRTNGEEPSKQLLFWSEY 98
F R + +G +P+ WS Y
Sbjct: 58 TGQFFGVSVHPDRWQFLVLEARDGADPAPADDGWSGY 94


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00612BACINVASINB300.009 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 30.1 bits (67), Expect = 0.009
Identities = 16/61 (26%), Positives = 24/61 (39%), Gaps = 2/61 (3%)

Query: 34 YEAYTKSDETPVTSADIAAHKL--ITERLSELTPDIPVLSEEAADISLEQRAQWERYWLV 91
YEA K +T + D A KL +L L P P ++ A + + E +
Sbjct: 143 YEASIKKTDTAKSVYDAATKKLTQAQNKLQSLDPADPGYAQAEAAVEQAGKEATEAKEAL 202

Query: 92 D 92
D
Sbjct: 203 D 203


42VIBHAR_00857VIBHAR_00868N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_00857-1121.314989undecaprenyl pyrophosphate phosphatase
VIBHAR_008580121.147692multifunctional tRNA nucleotidyl
VIBHAR_008591121.052305Type II secretory pathway, component ExeA
VIBHAR_00860-1120.426534Type II secretory pathway, component ExeA
VIBHAR_008610120.543997hypothetical protein
VIBHAR_008620101.593291hypothetical protein
VIBHAR_008631131.999510hypothetical protein
VIBHAR_008641132.014222hypothetical protein
VIBHAR_008650131.786001hypothetical protein
VIBHAR_008661121.929637hypothetical protein
VIBHAR_008670131.812208bifunctional glutamine-synthetase
VIBHAR_008681150.658301bifunctional heptose 7-phosphate kinase/heptose
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00857MICOLLPTASE330.001 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 33.2 bits (75), Expect = 0.001
Identities = 14/55 (25%), Positives = 21/55 (38%), Gaps = 1/55 (1%)

Query: 125 GLLLWWVDRNAELVADEYQTGWKKALFIGIAQALAMIPGTSRSGATITAALYLGF 179
G+L W+ + AE A +T K + Q LA S + A Y +
Sbjct: 527 GVLTWYEEGTAEFFAGSTRTDGIKPR-KSVTQGLAYDRNNRMSLYGVLHAKYGSW 580


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00859HTHFIS310.016 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.016
Identities = 16/43 (37%), Positives = 24/43 (55%)

Query: 47 MLTGEVGTGKTTVAKAMLANLDQNTKAGLILNPTFSSRDLLEA 89
M+TGE GTGK VA+A+ + + +N RDL+E+
Sbjct: 164 MITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00861GPOSANCHOR310.003 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 30.8 bits (69), Expect = 0.003
Identities = 15/65 (23%), Positives = 31/65 (47%), Gaps = 4/65 (6%)

Query: 95 VEKELKEVKEQLANARQNSDTEKAGLVTSLETRNQQISDLEKKYSEISDQLTSVETENRE 154
+E E ++++EQ + S+ + L L+ + +EK E + +L ++E N+E
Sbjct: 363 LEAEHQKLEEQN----KISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKE 418

Query: 155 LRAKL 159
L
Sbjct: 419 LEESK 423



Score = 29.3 bits (65), Expect = 0.012
Identities = 14/71 (19%), Positives = 32/71 (45%)

Query: 94 RVEKELKEVKEQLANARQNSDTEKAGLVTSLETRNQQISDLEKKYSEISDQLTSVETENR 153
+K+L+ ++L + S+ + L L+ + LE ++ ++ +Q E +
Sbjct: 323 EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQ 382

Query: 154 ELRAKLDTQKD 164
LR LD ++
Sbjct: 383 SLRRDLDASRE 393


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00863ANTHRAXTOXNA290.013 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 29.3 bits (65), Expect = 0.013
Identities = 34/181 (18%), Positives = 82/181 (45%), Gaps = 20/181 (11%)

Query: 29 ECCSHLVKFFEVSSKGDWEKAAEIRAQISHLEK-EADVLK--REIRLKLPRGLFMPVDRT 85
+ ++LVK E ++ E +I+ L+K DVL+ E+ ++ F +D
Sbjct: 61 DSINNLVKT-EFTN----ETLDKIQQTQDLLKKIPKDVLEIYSELGGEI---YFTDIDLV 112

Query: 86 DMLEL--LTQQDKLANLAKDIAGR---VYGRQLVIPEALQPNFLAYVQRCLDAADQAQKV 140
+ EL L++++K + + G R + + P + ++ ++Q+++V
Sbjct: 113 EHKELQDLSEEEKNS---MNSRGEKVPFASRFVFEKKRETPKLIINIKDYAINSEQSKEV 169

Query: 141 INELDELLETGFKGREVTLVAEMIHQLDVIEDDTDAMQIELRQQLM-AIEADMNPIDVMF 199
E+ + + ++ +L E ++ + + DD+D+ + Q+ +E + ID+ F
Sbjct: 170 YYEIGKGISLDIISKDKSLDPEFLNLIKSLSDDSDSSDLLFSQKFKEKLELNNKSIDINF 229

Query: 200 L 200
+
Sbjct: 230 I 230


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00866RTXTOXINA320.005 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 32.2 bits (73), Expect = 0.005
Identities = 14/93 (15%), Positives = 43/93 (46%)

Query: 167 SAQEEFNEIDQLATAMSEMSSTVQTVADHAQTASSLTEQASTQAVTGQQFLQKTVSKMSE 226
A+ + + + +S + + T + + +Q S V+ + + ++ +++
Sbjct: 131 GAENIGDNLGKAGGILSTFQNFLGTALSSMKIDELIKKQKSGGNVSSSELAKASIELINQ 190

Query: 227 LSSDIASSASAVNQVEERVDAIGSVVGTIQGIS 259
L +AS + VN ++++ +GSV+ + ++
Sbjct: 191 LVDTVASLNNNVNSFSQQLNTLGSVLSNTKHLN 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_00868LPSBIOSNTHSS300.014 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 29.8 bits (67), Expect = 0.014
Identities = 10/28 (35%), Positives = 15/28 (53%)

Query: 347 GCFDILHAGHVSYLNHAAELGDRLIVAV 374
G FD + GH+ + L D++ VAV
Sbjct: 7 GSFDPITFGHLDIIERGCRLFDQVYVAV 34


43VIBHAR_01129VIBHAR_01140N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01129022-0.352484hypothetical protein
VIBHAR_011300240.539758recombination and repair protein
VIBHAR_011311260.331961inorganic polyphosphate/ATP-NAD kinase
VIBHAR_011321270.105510heat shock protein GrpE
VIBHAR_011331230.287832hypothetical protein
VIBHAR_01134120-0.300568molecular chaperone DnaK
VIBHAR_01135-116-0.249714chaperone protein DnaJ
VIBHAR_01136-116-0.829522Tfp pilus assembly protein PilE
VIBHAR_01137-217-0.465002Tfp pilus assembly protein FimT
VIBHAR_01138-218-0.351439hypothetical protein
VIBHAR_01139-219-0.287314hypothetical protein
VIBHAR_011401200.311073Tfp pilus assembly protein PilV
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01129SACTRNSFRASE354e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 35.3 bits (81), Expect = 4e-05
Identities = 25/126 (19%), Positives = 44/126 (34%), Gaps = 9/126 (7%)

Query: 14 LVPIFVELEQYYFNEQAASEKELTHYLKYKVFSEY---SGVKVIAAYNDDTIMGFATYTV 70
++P F E + + E+ S+ Y + Y G Y ++ +G
Sbjct: 26 MIPAF-ENGVWTYTEERFSKPYFKQYEDDDMDVSYVEEEGKAAFLYYLENNCIGRIKIRS 84

Query: 71 MFPAPKLSGQMYLKDLFVSSSARGKGVGLKLMKYLTSLAVNHGCHRLDWTAESTNPIAGR 130
+ +G ++D+ V+ R KGVG L+ A + L + N A
Sbjct: 85 NW-----NGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACH 139

Query: 131 FYQSIG 136
FY
Sbjct: 140 FYAKHH 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01130RTXTOXIND340.001 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 34.4 bits (79), Expect = 0.001
Identities = 28/163 (17%), Positives = 58/163 (35%), Gaps = 27/163 (16%)

Query: 148 QYAGHLNLLKSTRSAYQHWRQADNNLKQLKENSQQNQAQKQLLEYQIKELNELSLGEEE- 206
+ +L+K S +Q N Q + N + +A++ + +I LS E+
Sbjct: 183 EVLRLTSLIKEQFSTWQ------NQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSR 236

Query: 207 -------FAE--------LEQEHKRLSNSGELAATCQQAIELIYEGEEVNALGILQSA-- 249
+ LEQE+K + EL Q ++ E L +
Sbjct: 237 LDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLF 296

Query: 250 -NHSLIQLAELDEKLAELPNMLSEAMIQLEETKNELRSYLDGI 291
N L +L + + + L L++ + + + +R+ +
Sbjct: 297 KNEILDKLRQTTDNIGLLTLELAKNEERQQAS--VIRAPVSVK 337


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01134SHAPEPROTEIN1408e-39 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 140 bits (354), Expect = 8e-39
Identities = 83/384 (21%), Positives = 145/384 (37%), Gaps = 79/384 (20%)

Query: 5 IGIDLGTTNSCVAVLDG----DKPRVIE-NAEGERTTASVVAYTDGETLVGQPAKRQAVT 59
+ IDLGT N+ + V ++P V+ + + SV A VG AK+
Sbjct: 13 LSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA-------VGHDAKQMLGR 65

Query: 60 NPTNTLFAIKRLIGRRFEDEEVQRDIEIMPYKIVKADNGDAWVEAQGQKMAAPQVSAEIL 119
P N + AI+ + D V +KM +
Sbjct: 66 TPGN-IAAIRPMKDGVIADFFVT------------------------EKM-----LQHFI 95

Query: 120 KKMKKTAEDFLGEEVTGAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYG 179
K++ + ++ VP +R+A +++ + AG +I EP AAA+ G
Sbjct: 96 KQVHS---NSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAG 152

Query: 180 LDKSGGDRTIAVYDLGGGTFDISIIEIDEVEGEKTFEVLATNGDTHLGGEDFDTRLINYL 239
L S V D+GGGT ++++I ++ V + +GG+ FD +INY+
Sbjct: 153 LPVS-EATGSMVVDIGGGTTEVAVISLNGV---------VYSSSVRIGGDRFDEAIINYV 202

Query: 240 VDEFNKEQGINLKNDPLAMQRVKEAAEKAKIELSSTSQTD----VNLPYVTADATGPKHM 295
+ G + AE+ K E+ S D + + P+
Sbjct: 203 RRNYGSLIG-------------EATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGF 249

Query: 296 NVKVTRAKLESLVEDLVQRSLEPLKVALADA--DLSVNDITD--VILVGGQTRMPMVQAK 351
+ + LE+L E L + + VAL +L+ +DI++ ++L GG + +
Sbjct: 250 TLN-SNEILEALQEPLTG-IVSAVMVALEQCPPELA-SDISERGMVLTGGGALLRNLDRL 306

Query: 352 VAEFFGKEARRDVNPDEAVAMGAA 375
+ E G +P VA G
Sbjct: 307 LMEETGIPVVVAEDPLTCVARGGG 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01135PF07132300.022 Harpin protein (HrpN)
		>PF07132#Harpin protein (HrpN)

Length = 356

Score = 29.7 bits (66), Expect = 0.022
Identities = 16/43 (37%), Positives = 18/43 (41%)

Query: 78 GGFGGGGFGGGGADFGDIFGDVFGDIFGGGRRGGGQQRAQRGA 120
G GGG GGG G G + G + GGG GG G
Sbjct: 62 GSMMGGGLGGGLGGLGSSLGGLGGGLLGGGLGGGLGSSLGSGL 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01136BCTERIALGSPG411e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 41.4 bits (97), Expect = 1e-07
Identities = 15/47 (31%), Positives = 31/47 (65%)

Query: 2 TLIELLIAVVIVGIIAAIAYPSYSNHSITAHRTVAISDIARLQLELE 48
TL+E+++ +VI+G++A++ P+ + A + A+SDI L+ L+
Sbjct: 11 TLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALD 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01137BCTERIALGSPH300.003 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 30.3 bits (68), Expect = 0.003
Identities = 14/48 (29%), Positives = 20/48 (41%), Gaps = 4/48 (8%)

Query: 17 RGFTLLELLNTIAVMSVLLVAAAPSFQNVFETKKMQRLADELHGFVLQ 64
RGFTLLE++ + +M V + F + A L F Q
Sbjct: 4 RGFTLLEMMLILLLMGVSAGMVLLA----FPASRDDSAAQTLARFEAQ 47


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01140BCTERIALGSPG332e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.6 bits (74), Expect = 2e-04
Identities = 17/58 (29%), Positives = 36/58 (62%), Gaps = 5/58 (8%)

Query: 4 KQKGFSLIEVLISFLLLGI-GALGLTKLQIYMER-ESDYAIQSIEALRLAENQLELFR 59
KQ+GF+L+E+++ +++G+ +L + L E+ + A+ I AL EN L++++
Sbjct: 6 KQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVAL---ENALDMYK 60


44VIBHAR_01287VIBHAR_01307N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_012872231.478714flagellar basal body rod protein FlgC
VIBHAR_012882211.759587flagellar basal body rod modification protein
VIBHAR_012893232.198209flagellar hook protein FlgE
VIBHAR_012901192.551426hypothetical protein
VIBHAR_01291-2142.372790flagellar basal body rod protein FlgF
VIBHAR_01292-1152.359286flagellar basal body rod protein FlgG
VIBHAR_01293-1151.849051flagellar basal body L-ring protein
VIBHAR_01294-2161.723415flagellar basal body P-ring protein
VIBHAR_012950141.205515flagellar rod assembly protein/muramidase FlgJ
VIBHAR_012960151.023806RNA-directed DNA polymerase
VIBHAR_012971140.957063flagellar hook-associated protein FlgK
VIBHAR_012980140.635462flagellar hook-associated protein FlgL
VIBHAR_012990140.173871hypothetical protein
VIBHAR_013001170.425581flagellin
VIBHAR_01301117-0.072202flagellin
VIBHAR_01302321-0.535472flagellin
VIBHAR_01303529-1.031298RNA-directed DNA polymerase
VIBHAR_01304430-0.703237hypothetical protein
VIBHAR_01305330-0.443566RNA-directed DNA polymerase
VIBHAR_01306233-0.883974PTS system glucose-specific transporter
VIBHAR_01307026-0.331804phosphoenolpyruvate-protein phosphotransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01287FLGHOOKAP1300.002 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 30.3 bits (68), Expect = 0.002
Identities = 9/32 (28%), Positives = 15/32 (46%)

Query: 98 NVNVMEEMANMISASRAYQTNLQVADSSKQML 129
VN+ EE N+ + Y N QV ++ +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIF 539


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01289FLGHOOKAP1388e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 37.6 bits (87), Expect = 8e-05
Identities = 14/33 (42%), Positives = 21/33 (63%)

Query: 3 YVSLSGLSSAQLDLNTTSNNIANANTYGFKESR 35
++SGL++AQ LNT SNNI++ N G+
Sbjct: 5 NNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37



Score = 34.9 bits (80), Expect = 6e-04
Identities = 11/49 (22%), Positives = 26/49 (53%)

Query: 389 TINNGMLEQSNIDMTQELVDLISAQRNFQANSRALEVHNQLQQNILQIR 437
++N S +++ +E +L Q+ + AN++ L+ N + ++ IR
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01292FLGHOOKAP1443e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.2 bits (104), Expect = 3e-07
Identities = 10/47 (21%), Positives = 22/47 (46%)

Query: 214 EIRQSMLEASNVNVTEELVNMIEAQRVYEMNSKVISSVDKMMSFVNQ 260
++ S VN+ EE N+ Q+ Y N++V+ + + + +
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544



Score = 39.6 bits (92), Expect = 8e-06
Identities = 17/77 (22%), Positives = 34/77 (44%), Gaps = 14/77 (18%)

Query: 5 LWVSKTGLDAQQTNIATISNNLANASTVGYKKSRAVFEDLFYQNINQPGGQSSQNTELPS 64
+ + +GL+A Q + T SNN+++ + GY + + + N+ L +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI--------------MAQANSTLGA 49

Query: 65 GLMLGAGSKVVATQKVH 81
G +G G V Q+ +
Sbjct: 50 GGWVGNGVYVSGVQREY 66


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01293FLGLRINGFLGH1483e-46 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 148 bits (375), Expect = 3e-46
Identities = 72/204 (35%), Positives = 105/204 (51%), Gaps = 13/204 (6%)

Query: 66 AWAPIHPKQK--------PEHYAAATGSLFSTEHITD-----LYDDSKPRGIGDIITVTL 112
AW P P + P A GS+F + + L++D +PR IGD +T+ L
Sbjct: 23 AWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVL 82

Query: 113 DETTRATKSAKADLSKTNDAKMEPLSVGGQELKIGGKYNFSYDLNNTNTFAGDSSAKQSN 172
E A+KS+ A+ S+ +V + G + + NTF G A SN
Sbjct: 83 QENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASN 142

Query: 173 SISGYITVEVIEVLANGNLVIRGEKWMTLNTGDEYIRLSGTIRPDDINFDNTIASNRVSN 232
+ SG +TV V +VL NGNL + GEK + +N G E+IR SG + P I+ NT+ S +V++
Sbjct: 143 TFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVAD 202

Query: 233 ARIQYSGTGSPQDMQEPGFLARFF 256
ARI+Y G G + Q G+L RFF
Sbjct: 203 ARIEYVGNGYINEAQNMGWLQRFF 226


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01294FLGPRINGFLGI416e-147 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 416 bits (1070), Expect = e-147
Identities = 166/366 (45%), Positives = 226/366 (61%), Gaps = 12/366 (3%)

Query: 4 ILLALLSVALFATTAQAARIKDVAQVAGVRSNQLVGYGLVSGLPGTGES---NPFTEQSF 60
+ AL ++ A +RIKD+A + R NQL+GYGLV GL GTG+S +PFTEQS
Sbjct: 12 VFSALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSM 71

Query: 61 AAMLQNFGIQMPPGTKPKIKNVAAVMVTATLPPFSKPGQQVDVTVSSIGSAKSLRGGTLL 120
AMLQN GI G KN+AAVMVTA LPPF+ PG +VDVTVSS+G A SLRGG L+
Sbjct: 72 RAMLQNLGITTQGGQ-SNAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLI 130

Query: 121 QTFLKGLDGQVYAVAQGNLVVSGFSAEGADGSKIVGNNPTVGLISSGATVEREIPNPFGR 180
T L G DGQ+YAVAQG L+V+GFSA+G D + + T + +GA +ERE+P+ F
Sbjct: 131 MTSLSGADGQIYAVAQGALIVNGFSAQG-DAATLTQGVTTSARVPNGAIIERELPSKFKD 189

Query: 181 GDYITFNLLDSDFTTAQRMADAVNNF----LGPQMASAVDATSVRVRAPRDVSQRVAFLS 236
+ L + DF+TA R+AD VN F G +A D+ + V+ PR V+ ++
Sbjct: 190 SVNLVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPR-VADLTRLMA 248

Query: 237 AIENLEFDPADGAAKIIVNSRTGTIVVGKHVRLKPAAVTHGGMTVAIKENLNVSQPNGFS 296
IENL + D AK+++N RTGTIV+G VR+ AV++G +TV + E+ V QP FS
Sbjct: 249 EIENLTVET-DTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFS 307

Query: 297 GGQTVVVPDSDIEVNEEKGKMFKFEPGLTLDDLVRAVNEVGAAPSDLMAILQALKQAGAI 356
GQT V P +DI +E K+ E G L LV +N +G ++AILQ +K AGA+
Sbjct: 308 RGQTAVQPQTDIMAMQEGSKVAIVE-GPDLRTLVAGLNSIGLKADGIIAILQGIKSAGAL 366

Query: 357 EGQLII 362
+ +L++
Sbjct: 367 QAELVL 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01295FLGFLGJ2741e-93 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 274 bits (701), Expect = 1e-93
Identities = 104/304 (34%), Positives = 161/304 (52%), Gaps = 28/304 (9%)

Query: 13 DISSLDSLRQKAVKEGKDGEHEA--LQAAARQFESIFTSMMLKSMREANEGFESNLMNSQ 70
D SL+ L+ KA GE A ++ ARQ E +F MMLKSMR+A + L +S+
Sbjct: 14 DAQSLNELKAKA------GEDPAANIRPVARQVEGMFVQMMLKSMRDALP--KDGLFSSE 65

Query: 71 NEKFYRQMLDEQMASNLSASGSMGLADMIVKQLTVGQ---GDDKAETAIRDAANNAVEYR 127
+ + Y M D+Q+A ++A +GLA+M+VKQ+T Q + ++ V Y+
Sbjct: 66 HTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLETVVRYQ 125

Query: 128 RVDPKKAREIEQRLIESGQLSRPGS--EPSRFDSPESFVNSMKPYADRAAKALGVEPSLL 185
Q L + Q + P + + DS ++F+ + A A++ GV L+
Sbjct: 126 ----------NQALSQLVQKAVPRNYDDSLPGDS-KAFLAQLSLPAQLASQQSGVPHHLI 174

Query: 186 LAQAALETGWGQKVVKNARGS-SNNLFNIKADRSWQGDKVTTQTLEFHDNTPVKETAAFR 244
LAQAALE+GWGQ+ ++ G S NLF +KA +W+G T E+ + K A FR
Sbjct: 175 LAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFR 234

Query: 245 SYSNYQDSFNDYVRFLNENPRYETALQQGGDSESFIRGIHSAGYATDPSYADKVLQVKQK 304
YS+Y ++ +DYV L NPRY A+ +E + + AGYATDP YA K+ + Q+
Sbjct: 235 VYSSYLEALSDYVGLLTRNPRY-AAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQ 293

Query: 305 IESM 308
++S+
Sbjct: 294 MKSI 297


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01297FLGHOOKAP1472e-163 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 472 bits (1217), Expect = e-163
Identities = 119/455 (26%), Positives = 214/455 (47%), Gaps = 18/455 (3%)

Query: 3 SDLLNVGTQSVLTAQRQLNTTGHNISNANTEGYSRQSVIQGTNDPRQFGGSTYGMGVHVE 62
S L+N + AQ LNT +NIS+ N GY+RQ+ I + G G GV+V
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVS 60

Query: 63 NVRRSWDEFAVKELNLSTTNYANKLDNEKNLDLLSSMLSSVASKKIPENMNEWFDAVKTM 122
V+R +D F +L + T + + + + +MLS+ ++ + M ++F +++T+
Sbjct: 61 GVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLST-STSSLATQMQDFFTSLQTL 119

Query: 123 ADTPNDMGARKVVLEKAKLLSDTLNEFHETVRQQSDVTNKSLDMGIERVNQLAVEIRDVH 182
D AR+ ++ K++ L + + +R Q N ++ ++++N A +I ++
Sbjct: 120 VSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLN 179

Query: 183 RLMMRTPG-----PHNDLMDQHEKLIKELSEYTKVTVTPRKNSEGFNVHIGNGHTLVSGT 237
+ R G N+L+DQ ++L+ EL++ V V+ + +N+ + NG++LV G+
Sbjct: 180 DQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGT-YNITMANGYSLVQGS 238

Query: 238 EASQLKMIDGSPDVHQRRLAMVEG--GGIKAIKSDDIDGKIGALLKMRDEHIPQVMDELG 295
A QL + S D + +A V+G G I+ + G +G +L R + + Q + LG
Sbjct: 239 TARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLG 298

Query: 296 RLATAFSSEVNHLQSQGLDHYGNVGDPLFTDVNSERLAKSRVVAGPQSTADMAV--YIND 353
+LA AF+ N G D G+ G+ F + K V+ ++ D+A+ + D
Sbjct: 299 QLALAFAEAFNTQHKAGFDANGDAGEDFFA------IGKPAVLQNTKNKGDVAIGATVTD 352

Query: 354 ISALKGGEYSLRYDGDSYAVTKPNGETVKLNT-DAGNAFYIDGMRVEVRNEPKVGEHVLL 412
SA+ +Y + +D + + VT+ T T DA DG+ + P V + L
Sbjct: 353 ASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTL 412

Query: 413 RPTRSSAAQMKVETTDPKAIAAQSYEASTTFAQGN 447
+P + M V TD IA S E + N
Sbjct: 413 KPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRN 447



Score = 135 bits (340), Expect = 9e-36
Identities = 35/105 (33%), Positives = 60/105 (57%)

Query: 533 EGDNGNLRKMQNIQTDKKMNGNESTIMSLYFNLNTDVGLKTSTASRLADVAQLESEAAQE 592
+ DN N + + ++Q++ K G + Y +L +D+G KT+T +
Sbjct: 442 DSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSN 501

Query: 593 RVASISGVNLDEEAANMMKFQQAYMASSRVMQAANDTFNTILQLR 637
+ SISGVNLDEE N+ +FQQ Y+A+++V+Q AN F+ ++ +R
Sbjct: 502 QQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01298FLAGELLIN363e-04 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 35.8 bits (82), Expect = 3e-04
Identities = 32/167 (19%), Positives = 60/167 (35%), Gaps = 3/167 (1%)

Query: 217 KSDSNYKVTFVDMSNGKFGYQLEKDGGIVQVDDYSPIDGIQYEGVNIQLKGQVSAGDSIT 276
+S N + V+ + + ++ + + G VN +AGD +T
Sbjct: 328 QSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVT 387

Query: 277 LEPRQTFSIFDAFKETMQWSESSVSDASATAKLHQMTEEFQAAFVHMNKVRTDVGAKLST 336
L ++F + + + A+A +A ++ VR+ +GA +
Sbjct: 388 L---AGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNR 444

Query: 337 LDIQEQNHEDFNLSLAKAKSNFEDLDYSKAVIEFSENSRALQASQQA 383
D N + +L A+S ED DY+ V S+ QA
Sbjct: 445 FDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSV 491



Score = 34.2 bits (78), Expect = 0.001
Identities = 25/135 (18%), Positives = 52/135 (38%), Gaps = 2/135 (1%)

Query: 9 HNYQSV--QNDLRRQENKVHHNQEQLASGKQLTKPSDDPLATHYIQSIGQQEEQLRQYLD 66
N S+ QN+L + ++ + E+L+SG ++ DD + L Q
Sbjct: 6 TNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASR 65

Query: 67 AIVLVRNRLENQEVAVANAEQFVDESKRLSMEMINGAFSPEDRAAKKRELEEIANNFMAL 126
+ + E A+ + + LS++ NG S D + + E+++ +
Sbjct: 66 NANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRV 125

Query: 127 ANSQDESGNYVFAGT 141
+N +G V +
Sbjct: 126 SNQTQFNGVKVLSQD 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01300FLAGELLIN1829e-55 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 182 bits (462), Expect = 9e-55
Identities = 89/370 (24%), Positives = 158/370 (42%), Gaps = 10/370 (2%)

Query: 2 AVTVSTNVSAMTAQRYLNKATNELNTSMERLSSGHKINSAKDDAAGLQISNRLTAQSRGL 61
A ++TN ++ Q LNK+ + L++++ERLSSG +INSAKDDAAG I+NR T+ +GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DVAMRNANDGISIAQTAEGAMNEATSVMQRMRDLAIQSANGTNSPAERKAINEESSALTD 121
A RNANDGISIAQT EGA+NE + +QR+R+L++Q+ NGTNS ++ K+I +E +
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 ELNRIAETTSFGGRRLLNGSFGEAAFQIGSSSGEAMIMALTSIRADDQRMGGVTFFSENG 181
E++R++ T F G ++L+ Q+G++ GE + + L I + G
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQ-MKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 KDKDWGVDPTKADLKITLPGMGEDEDGNPDDLEININAKTGDDIEELATYINGQSDMINA 241
+ + +++N+ T +
Sbjct: 180 ATVGD------LKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAAN 233

Query: 242 SVSEDGKLQVFVAHPNVQGDISISGGLASDLGLSDEAVRTSVQDIDMTTVQGSQNAISVL 301
+ A + S +G + D V + + +
Sbjct: 234 GQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGN 293

Query: 302 DSALK---YVDSQRADLGAKQNRLSHSINNLANIQENVDASNSRIKDTDFAKETTQMTKA 358
D K ++ ++ L + + A +Q + + S + + T+ A
Sbjct: 294 DGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESA 353

Query: 359 QILQQAGTSI 368
++ +
Sbjct: 354 KLSDLEANNA 363



Score = 133 bits (335), Expect = 1e-36
Identities = 71/241 (29%), Positives = 114/241 (47%), Gaps = 24/241 (9%)

Query: 162 TSIRADDQRMGGVTFFSENGKDKDWGVDPTKADLKITLPGMGEDEDGNPDDLEININAKT 221
D + T ++ G D + V T K+TL + N++A T
Sbjct: 273 KEGDTFDYKGVTFTIDTKTGNDGNGKVSTTINGEKVTL------TVADITAGAANVDAAT 326

Query: 222 GDDIEEL-ATYINGQSDMINASVSEDGKLQVFVAHPNVQGDISISGGLASDLGLSDEAVR 280
+ + + +NGQ + + +E KL A+ V+G+ I+ A +
Sbjct: 327 LQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKV 386

Query: 281 T-----------------SVQDIDMTTVQGSQNAISVLDSALKYVDSQRADLGAKQNRLS 323
T + + + + N ++ +DSAL VD+ R+ LGA QNR
Sbjct: 387 TLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFD 446

Query: 324 HSINNLANIQENVDASNSRIKDTDFAKETTQMTKAQILQQAGTSILAQAKQLPNSAMSLL 383
+I NL N N++++ SRI+D D+A E + M+KAQILQQAGTS+LAQA Q+P + +SLL
Sbjct: 447 SAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506

Query: 384 Q 384
+
Sbjct: 507 R 507


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01301FLAGELLIN1936e-59 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 193 bits (491), Expect = 6e-59
Identities = 86/297 (28%), Positives = 141/297 (47%), Gaps = 2/297 (0%)

Query: 2 AVNVITNVSAMTAQRYLNNANSAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGL 61
A + TN ++ Q LN + S+ +++ERLSSG +INSAKDDAAG I+NR +GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DVAVRNANDGISIAQTAEGAMNETTNILQRMRDLSLQSANGSNSKAERVAIQEEVTALND 121
A RNANDGISIAQT EGA+NE N LQR+R+LS+Q+ NG+NS ++ +IQ+E+ +
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 ELNRIAETTSFGGNKLLNGTYGTQSFQIGADNGEAVMLNLKDMRSDNKMMGGVSYQADNG 181
E++R++ T F G K+L+ Q+GA++GE + ++L+ + + + G +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQD-NQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 KDKNWNVEAGKNDLKISLTDSFGQEQEINISAKAGDDIEELATYINGQTDLVKASVDQDG 241
+ KN + +++++ A T +
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 242 KLQVFAGNNKVEGDVEFSGGLSGELGLNDGKKVTV-DTIDVTSVGGAQESVAIIDAA 297
+ + + +G + K DT D V ++ D
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGN 296



Score = 135 bits (341), Expect = 1e-37
Identities = 85/377 (22%), Positives = 141/377 (37%), Gaps = 21/377 (5%)

Query: 19 NNANSAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGLDVAVRNANDGISIAQTA 78
N Q + ++ G L + ++ + +
Sbjct: 132 NGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKN 191

Query: 79 EGAMNETTNILQRMRDLSLQSANGSNSKAERVAIQEEVTALNDELNRIAETTSFGGNKLL 138
+ + R A +++ A V + V A N +L + +
Sbjct: 192 VTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFK 251

Query: 139 NGTYGTQSFQIGADNGEAVMLNLKDMRSDNKMMGGVSYQADNGKDKNWNVEAGKNDLKIS 198
+ + A G D + G D N V N K++
Sbjct: 252 TTKSTAGTAEAKAIAGAIKGGKEGDTFDYKG--VTFTIDTKTGNDGNGKVSTTINGEKVT 309

Query: 199 LTDSFGQEQEINISAKAGDDIEEL-ATYINGQTDLVKASVDQDGKLQVFAGNNKVEGDVE 257
LT + N+ A + + + +NGQ + ++ KL NN V+G+ +
Sbjct: 310 LTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESK 369

Query: 258 FSGGLSGELGLNDGKKVTVD------------------TIDVTSVGGAQESVAIIDAALK 299
+ + G KVT+ + +A ID+AL
Sbjct: 370 ITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDSALS 429

Query: 300 YVDSHRAELGAFQNRFNHAISNLDNINENVNASKSRIKDTDFAKETTQMTKSQILSQASS 359
VD+ R+ LGA QNRF+ AI+NL N N+N+++SRI+D D+A E + M+K+QIL QA +
Sbjct: 430 KVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGT 489

Query: 360 SILAQAKQAPNSALSLL 376
S+LAQA Q P + LSLL
Sbjct: 490 SVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01302FLAGELLIN1505e-43 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 150 bits (379), Expect = 5e-43
Identities = 79/354 (22%), Positives = 132/354 (37%), Gaps = 17/354 (4%)

Query: 5 NTNVTAMMTQRHLGHAAEQNIESQRNLTSGYRINSASDDAAGLQISNTLNVQTRGLDVAL 64
NTN +++TQ +L + + L+SG RINSA DDAAG I+N +GL A
Sbjct: 5 NTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAS 64

Query: 65 RNAHDAYSVAQTAEGALHESSDILQRLRSLGLQAANGSNDSSDRQSIQYEVVALQDELDR 124
RNA+D S+AQT EGAL+E ++ LQR+R L +QA NG+N SD +SIQ E+ +E+DR
Sbjct: 65 RNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDR 124

Query: 125 VAITTTFADKNLFDGSYGSQSFHIGANANS-ISLTLRNMRSHIPEMGGQHYVG-DAVDKD 182
V+ T F + +GAN I++ L+ + + G + G
Sbjct: 125 VSNQTQFNGVKVLSQD-NQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVG 183

Query: 183 WRVTKDNQTLKLEYQDSKGKSQTEYIRLKLGDRIEQVATYINAQQSIVSASVTENSELQF 242
+ + + T + + + +
Sbjct: 184 DLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAEN 243

Query: 243 FASTLNAPEGVELKGSLADELDVTAGGLVTMDDIDMSTAGGAQLSIGVIDAAIKYVDSHR 302
+ G+ AG + + D G +I
Sbjct: 244 NTAVDLFKTTKSTAGTAEA--KAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDG------ 295

Query: 303 SEIGGFQNRISGTMDNLNTVNRSVTESKGRIRDTDFAREATEMVRSQVLQDATT 356
++S T++ + G ++++ V + V+ T
Sbjct: 296 ------NGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFT 343



Score = 100 bits (249), Expect = 3e-25
Identities = 46/216 (21%), Positives = 87/216 (40%), Gaps = 19/216 (8%)

Query: 177 DAVDKDWRVTKDNQTLKLEYQDSKGKSQTEYIRLKLGDRIEQ-VATYINAQQSIVSASVT 235
D + +V+ K+ + + + + + +N Q + +
Sbjct: 291 TGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKN 350

Query: 236 ENSELQFFASTLNAPEGVELKGSLADELDVTAGGLVTMD------------------DID 277
E+++L + ++ + A+ AG VT+ +
Sbjct: 351 ESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDA 410

Query: 278 MSTAGGAQLSIGVIDAAIKYVDSHRSEIGGFQNRISGTMDNLNTVNRSVTESKGRIRDTD 337
+ + ID+A+ VD+ RS +G QNR + NL ++ ++ RI D D
Sbjct: 411 AAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDAD 470

Query: 338 FAREATEMVRSQVLQDATTALLAQAKQRPSSALGLL 373
+A E + M ++Q+LQ A T++LAQA Q P + L LL
Sbjct: 471 YATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01303PF05616270.008 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 27.0 bits (59), Expect = 0.008
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 7/47 (14%)

Query: 15 RRVKKNKGCAGVD----KLDIDATIF---KLRQASNGQRFARVFWME 54
RR+ + GC GVD +L D + F K S +R AR +W +
Sbjct: 136 RRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMERLARPYWEK 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01307PHPHTRNFRASE7530.0 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 753 bits (1947), Expect = 0.0
Identities = 281/571 (49%), Positives = 409/571 (71%), Gaps = 2/571 (0%)

Query: 1 MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVARFFDARNKSAAQLETIKQK 60
I+GI AS G+AI KA + E + + +I+D V E+ + A KS +L IK +
Sbjct: 4 KITGIAASSGVAIAKAFIHLEPNVDIEKTSITD--VSTEIEKLTAALEKSKEELRAIKDQ 61

Query: 61 ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKNDKMTADHAIHSVIEEQACALESLDD 120
+ G +K IF H+++L+D EL + I I+N++M A++A+ V + ES+D+
Sbjct: 62 TEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDN 121

Query: 121 EYLKERATDIRDIGSRFVKNALGINIVSLSDIDQEVILVAYDLTPSETAQINLDYVLGFA 180
EY+KERA DIRD+ R + + +G+ SL+ I +E +++A DLTPS+TAQ+N +V GFA
Sbjct: 122 EYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFA 181

Query: 181 CDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAMNNKIVINPSDAELEEAK 240
DIGGRTSH++IM+RSLE+PA+VGT ++T+++++GDM+I+D + +++NP++ E++ +
Sbjct: 182 TDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYE 241

Query: 241 AVKAAFLAEKEELAKLKDLHAETTDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFL 300
+AAF +K+E AKL + T DG VE+ NIGT KD DG++ NGGEG+GLYRTEFL
Sbjct: 242 EKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFL 301

Query: 301 FMDRDALPTEEEQYQAYKEVAEAMNGEAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAV 360
+MDRD LPTEEEQ++AYKEV + M+G+ V+IRT+DIGGDK+L Y+ LPKE+NPFLG+RA+
Sbjct: 302 YMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFRAI 361

Query: 361 RISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIRELKQAIEEYKAELRAEGHAF 420
R+ L++++I R QLR +LRAS +G L++MFPMI ++EE+R+ K ++E K +L +EG
Sbjct: 362 RLCLEKQDIFRTQLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDV 421

Query: 421 DENIEIGVMVETPAAAAIAHHLAKEVAFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA 480
++IE+G+MVE P+ A A+ AKEV FFSIGTNDL QYT+A DR NE +S+LY P PA
Sbjct: 422 SDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPA 481

Query: 481 VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNS 540
+L ++ VI A+H+EGKW GMCGE+AGDE A LLLG+GLDEFSMS SI + +
Sbjct: 482 ILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKL 541

Query: 541 NFAEVKAMAEEALSLPTAAEIEAVVEKFIAE 571
+ E+K A++AL L TA E+E +V+K +
Sbjct: 542 SKEELKPFAQKALMLDTAEEVEQLVKKTYLK 572


45VIBHAR_01317VIBHAR_01326N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_013171171.165689hypothetical protein
VIBHAR_013182181.063101hypothetical protein
VIBHAR_013190192.143259short chain dehydrogenase
VIBHAR_013200181.330912hypothetical protein
VIBHAR_01321-1140.185908hypothetical protein
VIBHAR_01322-1110.298509phosphodiesterase
VIBHAR_01323-114-0.064040hypothetical protein
VIBHAR_01324-112-0.342388hypothetical protein
VIBHAR_01325-118-0.653081hypothetical protein
VIBHAR_01326-116-0.400624hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01317IGASERPTASE320.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.0 bits (72), Expect = 0.004
Identities = 17/87 (19%), Positives = 31/87 (35%), Gaps = 3/87 (3%)

Query: 168 PPADLTAAMNAQMKAERNKRAEILEAEGVRQAEILRAEGQKQSEILKAEGEKQSAILHAE 227
+ A + + + EI + A + +E AE KQ + +
Sbjct: 995 TNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTE-TVAENSKQESKTVEK 1053

Query: 228 ARERAAQAEAKATYMVSEAIAKGDVKA 254
+ A + A+ + E AK +VKA
Sbjct: 1054 NEQDATETTAQNREVAKE--AKSNVKA 1078


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01319DHBDHDRGNASE793e-19 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 78.6 bits (193), Expect = 3e-19
Identities = 49/217 (22%), Positives = 78/217 (35%), Gaps = 13/217 (5%)

Query: 3 KSVLITGCSTGIGYVCAHALQKECFHVIA----SCRQDQDIERLTNEGLTC--IKLDLND 56
K ITG + GIG A L + H+ A + ++ + L E D+ D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 57 PESISSGVAQALELAEGKLYAVFNNGAYGQPGALEDLPTVALQAQFQTNFFGWHQLVREV 116
+I A+ + + N +PG + L +A F N G R V
Sbjct: 69 SAAIDEITARIEREMGP-IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 117 LPHMRKQGEGRIIQNSSVLGFAAMKYRGAYNASKFALEGWTDTLRLELMDTNIKVALIEP 176
+M + G I+ S AY +SK A +T L LEL + NI+ ++ P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 177 GPIETQFRQNALKAFEQWIDVESSAHRAAYEGQKQRL 213
G ET + + W D + + +
Sbjct: 188 GSTETDMQWSL------WADENGAEQVIKGSLETFKT 218


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01320NUCEPIMERASE496e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 49.4 bits (118), Expect = 6e-09
Identities = 32/145 (22%), Positives = 50/145 (34%), Gaps = 20/145 (13%)

Query: 12 MKILLTGGTGFIGSELLKMLT--THQVILL---------TRTPARAKQRLQHADV----G 56
MK L+TG GFIG + K L HQV+ + + AR + L
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARL-ELLAQPGFQFHKI 59

Query: 57 NTEYLDSLDSLEDLNDVDAVINLAGEPIADKRWSQEQKEIICNSRWKVTERIVELIHAST 116
+ + + L + V R+S E +S I+E +
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHR--LAVRYSLENPHAYADSNLTGFLNILEGCRHNK 117

Query: 117 EPPSVFISGSAVGYYGDQQDHPFDE 141
++ S S+V YG + PF
Sbjct: 118 IQHLLYASSSSV--YGLNRKMPFST 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01321RTXTOXIND270.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 26.7 bits (59), Expect = 0.007
Identities = 3/26 (11%), Positives = 8/26 (30%)

Query: 35 SKFLSFIFWIVVLAGVGYVAWFYLVP 60
S+ + + ++ V L
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQ 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01326PF03309320.003 Bvg accessory factor
		>PF03309#Bvg accessory factor

Length = 271

Score = 31.7 bits (72), Expect = 0.003
Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 4/49 (8%)

Query: 238 EHHAAESSPVEVIATGGQNNQLILNYIHDSDHSYENVTL---RIFAGRN 283
+ + V V+ATG L+L + +H ++TL R+ RN
Sbjct: 212 DVDGFSGADVAVVATGH-TAPLVLPDLRTVEHYDRHLTLDGLRLVFERN 259


46VIBHAR_01438VIBHAR_01446N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01438-1140.902211hypothetical protein
VIBHAR_01439-1161.057882hypothetical protein
VIBHAR_01444-1151.056780****hypothetical protein
VIBHAR_014450151.180933hypothetical protein
VIBHAR_014460160.004759hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01438HTHTETR389e-06 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 38.1 bits (88), Expect = 9e-06
Identities = 16/106 (15%), Positives = 29/106 (27%), Gaps = 5/106 (4%)

Query: 1 MARRNDHTREELVNLTLSTVKDFLEENSYHELSLRKIANMIGYVPSTLVNIFGNYNLLLL 60
+ TR+ + L + SL +IA G + F + + L
Sbjct: 5 TKQEAQETRQHI----LDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 HAVAQTLDELQQ-EAVAATKNSKDTHQALFELAYCYHDFAQKHPHR 105
+ + + E K D L E+ + R
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERR 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01444RTXTOXIND484e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.5 bits (113), Expect = 4e-08
Identities = 29/210 (13%), Positives = 75/210 (35%), Gaps = 21/210 (10%)

Query: 54 VKEIAVKANQNVQQDQLLILLNDDKAQ--AALVEAKAYLKDEQRKLKEFQRLVKRNAITQ 111
+ + A+ + ++Q+ + ++ + L + ++ + + + + +L K + +
Sbjct: 244 LHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDK 303

Query: 112 TEIDAQKASVEIAEARLDAAKANLSDLNISAPFAGTVGFID-FSRGKMVSAGSELLTL-D 169
+ L + I AP + V + + G +V+ L+ +
Sbjct: 304 LR-QTTDNIGLL-TLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVP 361

Query: 170 DLSVMELDLQVPERYLSMLSVGMEVHATTSAWNGVDFVGKVTGIDTRISAETL------- 222
+ +E+ V + + ++VG A+ + G + G I+ + +
Sbjct: 362 EDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRY-GYLVGKVKNINLDAIEDQRLGL 420

Query: 223 --NLRIRIEFDNPENH-----LKPGMLMQA 245
N+ I IE + L GM + A
Sbjct: 421 VFNVIISIEENCLSTGNKNIPLSSGMAVTA 450



Score = 38.3 bits (89), Expect = 4e-05
Identities = 18/97 (18%), Positives = 46/97 (47%), Gaps = 3/97 (3%)

Query: 43 SVVVASEVSGKVKEIAVKANQNVQQDQLLILLNDDKAQAALVEAKAYL---KDEQRKLKE 99
S + + VKEI VK ++V++ +L+ L A+A ++ ++ L + EQ + +
Sbjct: 96 SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQI 155

Query: 100 FQRLVKRNAITQTEIDAQKASVEIAEARLDAAKANLS 136
R ++ N + + ++ + ++E + + +
Sbjct: 156 LSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIK 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01445ACRIFLAVINRP7740.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 774 bits (1999), Expect = 0.0
Identities = 307/1032 (29%), Positives = 516/1032 (50%), Gaps = 28/1032 (2%)

Query: 3 LSDVSVKRPVAALVLSMLLCVFGFVSFTKLAVREMPDIESPVVSISTRYEGASATIIESQ 62
+++ ++RP+ A VL+++L + G ++ +L V + P I P VS+S Y GA A ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 ITSVLEDQLSGISGIDEISSTTRNSMS-RITITFELGYDLNTGVSDVRDAVARAQRSLPD 121
+T V+E ++GI + +SST+ ++ S IT+TF+ G D + V++ + A LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 EADDPVVFKNNGSGEASLYINLSSSEMDRTQ--LTDYAERVLMDRFSLISGVSSIDLSGG 179
E + S + S TQ ++DY + D S ++GV + L G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 180 LYKVMYVKLKPDLMAGRSVTASDITAALRSENLESPGGEVRNDSTV------MSVRTART 233
Y + + L DL+ +T D+ L+ +N + G++ + S+
Sbjct: 181 QYAMR-IWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 234 YNTPEDFQYLVVKRASDNTPIYLKDVADVFIGAENENSTFKSDGVVNISLGVVPQSDANP 293
+ PE+F + ++ SD + + LKDVA V +G EN N + +G LG+ + AN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 LEVAQSVRSEVDKIQKFLPDGTRLAIDYDATVFIERSIDEVYSTLFITGGLVILVLYIFI 353
L+ A+++++++ ++Q F P G ++ YD T F++ SI EV TLF LV LV+Y+F+
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 GQARATLIPAVTVPVSLISSFIAAYYFGFSINLITLMALILSIGLVVDDAIVVVENIFHH 413
RATLIP + VPV L+ +F FG+SIN +T+ ++L+IGL+VDDAIVVVEN+
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 I-ERGESPLLAAYKGTREVGFAVIATTLVLVMVFLPISFMDGMVGLLFTEFSVLLAMSVI 472
+ E P A K ++ A++ +VL VF+P++F G G ++ +FS+ + ++
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 FSSLIALTLTPVLGSQILKANVK-----PNRFNQAVDKLFAKLEAGYRKALRGALKVRWA 527
S L+AL LTP L + +LK F + F Y ++ L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 528 APLVIVACMGGSYGLMNQVPTQLTPQEDRGVIFAFVRGADATSYNRMSANMDIVEDRLMP 587
L+ + G L ++P+ P+ED+GV ++ + R +D V D +
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 588 LLGQGFLKSFSIQTPAFGGQAGDQTGFVIMILEDWNERDV---TAQEALNQVRKSLAGIP 644
F++ +F GQ G + L+ W ER+ +A+ +++ + L I
Sbjct: 600 NEKANVESVFTVNGFSFSGQ-AQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 645 DVRVFPF-MPGFRG-GSSEPVQFVL---GGSDYDELLVWAEMLKNKAEESP-MMEGAEID 698
D V PF MP G++ F L G +D L L A + P + +
Sbjct: 659 DGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 699 YSEKTPELLVSVDKRRAAELGVSVQDISETLEIMLGGKSETTYVDRGEEYDVYLRGDENS 758
E T + + VD+ +A LGVS+ DI++T+ LGG ++DRG +Y++ D
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 759 FNNAADLSQIYLRTTSGELVTLDTVTKIEEVAASIRLSHYNKQKSITITANLSEGYTLGE 818
D+ ++Y+R+ +GE+V T V S RL YN S+ I + G + G+
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 819 ALDFLDQEAIDNLPGDISVSYSGESKDFKENQASVAVVFALALLVAYLVLAAQFESFVNP 878
A+ ++ A LP I ++G S + + + A++ +V +L LAA +ES+ P
Sbjct: 839 AMALMENLA-SKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIP 897

Query: 879 LVVMFTVPMGVFGGFLGLVVMSQGMNIYSQIGMIMLIGMVTKNGILIVEFANQLRDR-GI 937
+ VM VP+G+ G L + +Q ++Y +G++ IG+ KN ILIVEFA L ++ G
Sbjct: 898 VSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGK 957

Query: 938 EFEKAITDAAARRLRPIMMTAFTTLAGAIPLIMSTGAGYESRVAVGTVIFFGMGFATLVT 997
+A A RLRPI+MT+ + G +PL +S GAG ++ AVG + GM ATL+
Sbjct: 958 GVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLA 1017

Query: 998 LFVIPAMYRLIS 1009
+F +P + +I
Sbjct: 1018 IFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01446OMPADOMAIN382e-05 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 37.6 bits (87), Expect = 2e-05
Identities = 37/204 (18%), Positives = 64/204 (31%), Gaps = 29/204 (14%)

Query: 2 KNAILLLSALTMSLPTFAAQEQTHQSYLYLGTGNVGFDTP----DFGSDRDSGWSTPHIT 57
K AI + AL A + + Y G + + G ++ +
Sbjct: 3 KTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTHEN-----QLG 57

Query: 58 VG--WGYHVNQYLAFE-GALRYSQNELKNDALKTDLDLHYYQAGMSAVVTSDNLGDTPLS 114
G GY VN Y+ FE G + K + + Q + D L
Sbjct: 58 AGAFGGYQVNPYVGFEMGYDWLGRMPYKG---SVENGAYKAQGVQLTAKLGYPITDD-LD 113

Query: 115 LFGRVTALGTQAEMYVPNEQKI-----ADDTGALFNIGAGIHWDMSKDIWLRAEYIY--N 167
++ R+ M + K DTG G+ + ++ +I R EY + N
Sbjct: 114 IYTRLGG------MVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNN 167

Query: 168 VADMGFDDFYDSYEGVQVSLGKRF 191
+ D + + + RF
Sbjct: 168 IGDAHTIGTRPDNGMLSLGVSYRF 191


47VIBHAR_01700VIBHAR_01713N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01700-2232.163119hypothetical protein
VIBHAR_017011251.955399hypothetical protein
VIBHAR_017024210.916557hypothetical protein
VIBHAR_017033200.558919hypothetical protein
VIBHAR_017043221.099902hypothetical protein
VIBHAR_017053210.681482hypothetical protein
VIBHAR_017060221.845852hypothetical protein
VIBHAR_017071231.556062hypothetical protein
VIBHAR_017081231.515616hypothetical protein
VIBHAR_017090221.016080type III secretion system protein
VIBHAR_017100200.806029hypothetical protein
VIBHAR_01711-1200.125673hypothetical protein
VIBHAR_01712021-2.143726hypothetical protein
VIBHAR_01713-221-1.234745effector protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01700TYPE3OMGPROT5820.0 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 582 bits (1502), Expect = 0.0
Identities = 296/603 (49%), Positives = 412/603 (68%), Gaps = 17/603 (2%)

Query: 16 VACTLSLLSALPLHASELDWPDQPFRYYADNDSLKELLNNFGANYRVSVSVSDKVNDRVS 75
+ TL LLS+ A ELDW P+ Y A +SL++LL +FGANY +V VSDK+ND+VS
Sbjct: 13 LTGTLLLLSS-YSWAQELDWLPIPYVYVAKGESLRDLLTDFGANYDATVVVSDKINDKVS 71

Query: 76 GRFTPEDPAEFLDYLAQVYNLMWYFDGAVLHVYKATETRSRLLQLELLTARELRSTLIST 135
G+F ++P +FL ++A +YNL+WY+DG VL+++K +E SRL++L+ A EL+ L +
Sbjct: 72 GQFEHDNPQDFLQHIASLYNLVWYYDGNVLYIFKNSEVASRLIRLQESEAAELKQALQRS 131

Query: 136 GVWDSRYGWRAAENKGLVYLAGPPRYVDLVVQTAEALESRLVQKSNSTDELFVEIIPLKY 195
G+W+ R+GWR + LVY++GPPRY++LV QTA ALE + +S T L +EI PLKY
Sbjct: 132 GIWEPRFGWRPDASNRLVYVSGPPRYLELVEQTAAALEQQTQIRSEKTGALAIEIFPLKY 191

Query: 196 ASATDREITYRDQAVTVPGIASVLSRVLSGVQTQIKATKESEETDINTAATTTKNVSVHG 255
ASA+DR I YRD V PG+A++L RVLS Q + I AAT
Sbjct: 192 ASASDRTIHYRDDEVAAPGVATILQRVLSDATIQ---QVTVDNQRIPQAATRAS-----A 243

Query: 256 GASVEAEPGLNAIIVRDTQARLPLYRKLVAQLDQPQSRIEVALSIVDISANDLSQLGVDW 315
A VEA+P LNAIIVRD+ R+P+Y++L+ LD+P +RIEVALSIVDI+A+ L++LGVDW
Sbjct: 244 QARVEADPSLNAIIVRDSPERMPMYQRLIHALDKPSARIEVALSIVDINADQLTELGVDW 303

Query: 316 RAGIEVGNNSILDIKTTGDVDGGDVTLGSGQTFKSLLDATNLNYLLAQIRLLESQGSAQV 375
R GI GNN + IKTTGD + S SL+DA L+YLLA++ LLE++GSAQV
Sbjct: 304 RVGIRTGNNHQVVIKTTGDQSN----IASNGALGSLVDARGLDYLLARVNLLENEGSAQV 359

Query: 376 VSRPTLLTQENVEAVLNNSSTFYIKLIGRETAALEEVTYGTLLRIVPRIVGDRFVERPEI 435
VSRPTLLTQEN +AV+++S T+Y+K+ G+E A L+ +TYGT+LR+ PR++ ++ EI
Sbjct: 360 VSRPTLLTQENAQAVIDHSETYYVKVTGKEVAELKGITYGTMLRMTPRVLTQG--DKSEI 417

Query: 436 NLSLHLEDGAQIPDG-GVDDVPSIRKTEISTLATVKQGQSLLIGGVYRDEVSHQLRKVPL 494
+L+LH+EDG Q P+ G++ +P+I +T + T+A V GQSL+IGG+YRDE+S L KVPL
Sbjct: 418 SLNLHIEDGNQKPNSSGIEGIPTISRTVVDTVARVGHGQSLIIGGIYRDELSVALSKVPL 477

Query: 495 LGDIPYLGALFRSTTDTTRRTVRMFIIEPRIVVDGIGDNVLIGNEYDLRPKLGTLNNISN 554
LGDIPY+GALFR ++ TRRTVR+FIIEPRI+ +GI ++ +GN DLR + T++ ISN
Sbjct: 478 LGDIPYIGALFRRKSELTRRTVRLFIIEPRIIDEGIAHHLALGNGQDLRTGILTVDEISN 537

Query: 555 NSAELKSALALYSCTSQVQAERHQQDLLSQGKSSLLSQCELPSGQKGWRVQIKECDGSEN 614
S L L C +A+ Q+ L KSS L+QC++ GWRV C +++
Sbjct: 538 QSTTLNKLLGGSQCQPLNKAQEVQKWLSQNNKSSYLTQCKM-DKSLGWRVVEGACTPAQS 596

Query: 615 HCV 617
CV
Sbjct: 597 WCV 599


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01703TRNSINTIMINR290.002 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 28.5 bits (63), Expect = 0.002
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 3/40 (7%)

Query: 13 DDVKAKLEEQAKAANEAVNEAIKNLETNADDPSKLAELQH 52
DD+ ++ +QAK A E + + +E+NA + + QH
Sbjct: 315 DDIVEQIAQQAKEAGEVARQ--QAVESNAQAQQRYED-QH 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01705PF09025613e-14 YopR Core
		>PF09025#YopR Core

Length = 143

Score = 61.2 bits (148), Expect = 3e-14
Identities = 38/123 (30%), Positives = 58/123 (47%), Gaps = 9/123 (7%)

Query: 101 PSEQKEALWYALSQERSVKGTDKASSELLASLEQELLGSFSGRLLADP----PKDREELK 156
P+ + + E+++ G A+ LA LE LG + A P DR ELK
Sbjct: 24 PAVDQVLAF-----EQALGGEPPAAGRRLAGLENGALGERLLQRFAQPLQGLEADRLELK 78

Query: 157 VLLSEHFPLGAQKEQALWHSWAELKDIPDQEPLLDMVREELSFVIQINSMVKNMLTYSHK 216
+L PLG Q++ L ++ P E L + R EL +I +N M+ N++ SHK
Sbjct: 79 AMLRAELPLGRQQQTFLLQLLGAVEHAPGGEYLAQLARRELQVLIPLNGMLDNLVRNSHK 138

Query: 217 LEL 219
L+L
Sbjct: 139 LDL 141


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01707FLGMRINGFLIF653e-14 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 65.0 bits (158), Expect = 3e-14
Identities = 32/168 (19%), Positives = 72/168 (42%), Gaps = 8/168 (4%)

Query: 26 TELYTGVSQKEGNEMLSILLSEGVVATKEPDKDNTVKLMVDNAQIAFAVDALKRKGYPRE 85
L++ +S ++G +++ L + + + V ++ L ++G P+
Sbjct: 51 RTLFSNLSDQDGGAIVAQLTQMNIPYRFANG---SGAIEVPADKVHELRLRLAQQGLPKG 107

Query: 86 QFSTLKEVFPKDDLISSPLAERARLIYAKSQELSSTLSQIDGVLVARVHVVL-EDQNLRP 144
+ E+ ++ S +E+ A EL+ T+ + V ARVH+ + +
Sbjct: 108 G-AVGFELLDQEKFGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVR 166

Query: 145 GERPTPASASVFIKHAADVALD-SYVPQIKLLVNNSIEGLNYDRISVV 191
++ SASV + ALD + + LV++++ GL +++V
Sbjct: 167 EQK--SPSASVTVTLEPGRALDEGQISAVVHLVSSAVAGLPPGNVTLV 212


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01709FLGFLIH351e-04 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 34.8 bits (79), Expect = 1e-04
Identities = 37/156 (23%), Positives = 72/156 (46%), Gaps = 18/156 (11%)

Query: 52 EAYELERQRGYQDGIYQAKIENAETMLATLARCNEYYLQVE-------QKMTGVVLDAVR 104
+ Y+ +G + G+ +AK + A +E+ ++ ++ + L+A R
Sbjct: 70 QGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAAR 129

Query: 105 KIINSFDDVDTTASV--VREALQ---LVSNQKQVILHVHPEQVTEVREKVSSILSDFPEV 159
++I VD +A + +++ LQ L S + Q L VHP+ + V + + + LS +
Sbjct: 130 QVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQ--LRVHPDDLQRVDDMLGATLS----L 183

Query: 160 GYVEVIADARLKNGGCILETEIGIIDASIDGQLQAL 195
+ D L GGC + + G +DAS+ + Q L
Sbjct: 184 HGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01712PF05932643e-16 Tir chaperone protein (CesT)
		>PF05932#Tir chaperone protein (CesT)

Length = 127

Score = 64.1 bits (156), Expect = 3e-16
Identities = 39/123 (31%), Positives = 65/123 (52%), Gaps = 10/123 (8%)

Query: 1 MVNGFISTLLNDFAKRCQIETLEFDEEGCCRLIIDNEVAITL--RSNEEKLTLIGLISGE 58
M N F TLL+DF++ +++ L FD+ G C +IIDN A+TL E+L LIGL+
Sbjct: 1 MSNLFYKTLLDDFSRSLEMQPLVFDDHGTCNMIIDNTFALTLSCDYARERLLLIGLLE-- 58

Query: 59 KPHPDVYFKHMKAA----LTKDEPYVCWDDDAG-YIGFIHIHQTMLTETYFETSIAQFVD 113
PH D+ + + A L P + D+ +G Y + I + L+ + +A ++
Sbjct: 59 -PHKDIPQQCLLAGALNPLLNAGPGLGLDEKSGLYHAYQSIPREKLSVPTLKREMAGLLE 117

Query: 114 WLK 116
W++
Sbjct: 118 WMR 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01713YERSSTKINASE340.001 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 33.9 bits (77), Expect = 0.001
Identities = 24/70 (34%), Positives = 40/70 (57%), Gaps = 4/70 (5%)

Query: 25 GKLNVGGKEYKIDADTQQFVRSNPSDSAVARFFEATGKLFREGNTD-SVAKAITKSVFDN 83
G+LN+GGK Y+I + Q +R NP S + F E GK+F + S+A+ +T ++
Sbjct: 32 GELNIGGKRYRIIDN--QVLRLNPH-SGFSLFREGVGKIFSGKMFNFSIARNLTDTLHAA 88

Query: 84 ELGQAQRLQS 93
+ +Q L+S
Sbjct: 89 QKTTSQELRS 98


48VIBHAR_01720VIBHAR_01731N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_017201210.324489hypothetical protein
VIBHAR_01721222-0.259456hypothetical protein
VIBHAR_017221210.464194transcriptional regulator
VIBHAR_017230211.455576Type III secretory pathway, component EscU
VIBHAR_017242191.229471Type III secretory pathway, component EscT
VIBHAR_017252202.883471Type III secretory pathway, component EscS
VIBHAR_017262182.372695type III secretion system protein
VIBHAR_017273162.186376type III secretion system protein
VIBHAR_017284172.622109hypothetical protein
VIBHAR_017292202.659617hypothetical protein
VIBHAR_017302202.666826type III secretion system ATPase
VIBHAR_01731-1170.968563hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01720PF05932411e-06 Tir chaperone protein (CesT)
		>PF05932#Tir chaperone protein (CesT)

Length = 127

Score = 40.9 bits (96), Expect = 1e-06
Identities = 20/105 (19%), Positives = 36/105 (34%), Gaps = 5/105 (4%)

Query: 6 FSEEGRCQLLVDDRYVIYFSATDNNDLILSVAFGGLEKTGELRVKGLELIAHANYFAIAE 65
F + G C +++D+ + + S D L + G LE ++ + + +
Sbjct: 24 FDDHGTCNMIIDNTFALTLSC-DYARERL-LLIGLLEPHKDIP---QQCLLAGALNPLLN 78

Query: 66 GGLTLSLAPNGRQLVLAGRKPIEHLDAASLSTWFHALNEQAQLWQ 110
G L L P E L +L L E + W+
Sbjct: 79 AGPGLGLDEKSGLYHAYQSIPREKLSVPTLKREMAGLLEWMRGWR 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01723TYPE3IMSPROT415e-148 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 415 bits (1069), Expect = e-148
Identities = 212/346 (61%), Positives = 279/346 (80%)

Query: 1 MSGEKTELPTPKKLRDARQKGQVAKSQEVVSSALILALIAVLFAFADYYMSHISALLLLP 60
MSGEKTE PTPKK+RDAR+KGQVAKS+EVVS+ALI+AL A+L +DYY H S L+L+P
Sbjct: 1 MSGEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIP 60

Query: 61 SELAYQGFQDALLDVAIAIAKEMVYLLAPILIVAALIAIMSNMGQFGLLFSGESVKPDIK 120
+E +Y F AL V + E YL P+L VAAL+AI S++ Q+G L SGE++KPDIK
Sbjct: 61 AEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIK 120

Query: 121 KINPVEGAKRIFSLKSIIEFIKSILKVSLLSCIIWVTLRGNLNTLLQIPTCGLECVPTIT 180
KINP+EGAKRIFS+KS++EF+KSILKV LLS +IW+ ++GNL TLLQ+PTCG+EC+ +
Sbjct: 121 KINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLL 180

Query: 181 GVLVKQLMIISSVGFIVIAAADYAYQKFDHTKQLKMTKDEVKREYKEMEGSPEIKSKRRQ 240
G +++QLM+I +VGF+VI+ ADYA++ + + K+LKM+KDE+KREYKEMEGSPEIKSKRRQ
Sbjct: 181 GQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQ 240

Query: 241 LHQELQASNQRDNVKRSNVLVTNPTHIAIGLYYKKGETPLPVITLKETDAMAKRMIAIAH 300
HQE+Q+ N R+NVKRS+V+V NPTHIAIG+ YK+GETPLP++T K TDA + + IA
Sbjct: 241 FHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAE 300

Query: 301 EEGIPVMQKVPLARALYADGQLNQYIPGDLIEATAEILRWVASLEQ 346
EEG+P++Q++PLARALY D ++ YIP + IEATAE+LRW+
Sbjct: 301 EEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNI 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01724TYPE3IMRPROT1573e-49 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 157 bits (398), Expect = 3e-49
Identities = 52/249 (20%), Positives = 110/249 (44%), Gaps = 5/249 (2%)

Query: 10 LFLYSLTLPRLMACFIFLPILNKQTLGGTLVRNGVLCSLALFIFPMINQQDLPAETDGIW 69
L LY L R++A PIL+++++ V+ G+ + I P + D+P +
Sbjct: 13 LNLYFWPLLRVLALISTAPILSERSVPKR-VKLGLAMMITFAIAPSLPANDVPVFSFF-- 69

Query: 70 LMVILGKEVLLGMLIGFVAAIPFWAIEAAGFLVDNQRGAAMASMFNPTLGSQSTPTAVLL 129
+ + +++L+G+ +GF F A+ AG ++ Q G + A+ +P A ++
Sbjct: 70 ALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIM 129

Query: 130 TQTLITLFFSGGGFVAFIYALFNSYSSWPVISFFPTVTEEWVRFFYAQFEQLMWLGVLMS 189
+ LF + G + I L +++ + P+ + G++++
Sbjct: 130 DMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPL--NSNAFLALTKAGSLIFLNGLMLA 187

Query: 190 APLVLAMFLAEFGLALISRFAPQLNVFSLAMPIKSAIASVLLIVYLALMMDHFQALFSDI 249
PL+ + L L++R APQL++F + P+ + L+ + L+ + LFS+I
Sbjct: 188 LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEI 247

Query: 250 VLFREQLNT 258
+ +
Sbjct: 248 FNLLADIIS 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01725TYPE3IMQPROT635e-17 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 62.9 bits (153), Expect = 5e-17
Identities = 32/73 (43%), Positives = 47/73 (64%)

Query: 5 EIIHFTTQALTLVLFLSLPPILVAALVGTLVSLVQALTQVQEQTLGFVVKLIAVIITLFV 64
+++ +AL LVL LS P +VA ++G LV L Q +TQ+QEQTL F +KL+ V + LF+
Sbjct: 3 DLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFL 62

Query: 65 TTQWLGAELHSFA 77
+ W G L S+
Sbjct: 63 LSGWYGEVLLSYG 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01726TYPE3IMPPROT2513e-87 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 251 bits (642), Expect = 3e-87
Identities = 97/217 (44%), Positives = 143/217 (65%), Gaps = 7/217 (3%)

Query: 6 DELNLIVTLALLALIPFIAMMATSFVKLAVVFSLLRNALGVQQIPPNMALYGLAIILSIF 65
++++LI LA L+PFI T FVK ++VF ++RNALG+QQIP NM L G+A++LS+F
Sbjct: 3 NDISLIALLAFSTLLPFIIASGTCFVKFSIVFVMVRNALGLQQIPSNMTLNGVALLLSMF 62

Query: 66 IMAPVGFETYDYVTQHEISLEDSESIEGLIESGLQPYREFLKKHIRETESVFFTDAARTL 125
+M P+ + Y Y +++ D S+ ++ GL YR++L K+ FF +A
Sbjct: 63 VMWPIMHDAYVYFEDEDVTFNDISSLSKHVDEGLDGYRDYLIKYSDRELVQFFENAQLKR 122

Query: 126 WP-------QKYVDRLESDSLLLLLPAFTVSELTRAFEIGFLLYLPFIAIDLIVSNILLA 178
++ D +E S+ LLPA+ +SE+ AF+IGF LYLPF+ +DL+VS++LLA
Sbjct: 123 QYGEETETVKRDKDEIEKPSIFALLPAYALSEIKSAFKIGFYLYLPFVVVDLVVSSVLLA 182

Query: 179 MGMMMVSPMTISLPFKLLLFVLLDGWTKLTHGLVLSY 215
+GMMM+SP+TIS P KL+LFV LDGWT L+ GL+L Y
Sbjct: 183 LGMMMMSPVTISTPIKLVLFVALDGWTLLSKGLILQY 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01727TYPE3OMOPROT849e-21 Type III secretion system outer membrane O protein ...
		>TYPE3OMOPROT#Type III secretion system outer membrane O protein

family signature.
Length = 303

Score = 84.3 bits (208), Expect = 9e-21
Identities = 42/165 (25%), Positives = 73/165 (44%), Gaps = 7/165 (4%)

Query: 154 DTLPQAPSYL-----SQNIALPFWLSFGKTRLALSQFEQLELGDVVFFDDCYIAQHQVLL 208
+ LP+ P+ L F + T+ +L ++ +GDV+ +
Sbjct: 132 EHLPELPAVGGGRPKMLRWPLRFVIGSSDTQRSL--LGRIGIGDVLLIRTSRAEVYCYAK 189

Query: 209 QVSNHNLWRCQLDNTTLHIQEKETNMNDINSSEALTDHQQLPIELTFDVGQQTITLEQLN 268
++ + N + TL IQ E N ++E L QLP++L F + ++ +TL +L
Sbjct: 190 KLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYRKNVTLAELE 249

Query: 269 ALQPGFTFELNQPISNPVTMRANGKIIGECELVNINERLGVRVLE 313
A+ L V + ANG ++G ELV +N+ LGV + E
Sbjct: 250 AMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHE 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_0172960KDINNERMP270.044 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 26.8 bits (59), Expect = 0.044
Identities = 13/43 (30%), Positives = 25/43 (58%)

Query: 60 KQKTVVLKELEILRQEIALLREREADLKQRAAEEKKALEQENQ 102
K + + ++ +L+ +I +RER D KQR ++E AL + +
Sbjct: 374 KAQYTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEK 416


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01731PF07201295e-102 Hypersensitivity response secretion protein HrpJ
		>PF07201#Hypersensitivity response secretion protein HrpJ

Length = 293

Score = 295 bits (757), Expect = e-102
Identities = 96/292 (32%), Positives = 163/292 (55%), Gaps = 5/292 (1%)

Query: 1 MSTINSQILTGNKFDAPIRSNLESSRTGASVNGNYRGETVRVHNAT-QSLFDAMEELTAL 59
M+T+++ + GN R + SS+ G +RGE+V++ + T QS+ D EE+T +
Sbjct: 1 MTTLHN-LSYGNTPLHNERPEIASSQIVNQTLGQFRGESVQIVSGTLQSIADMAEEVTFV 59

Query: 60 GSEKAEKDLTKRKVKDGSIRVNEAHELVSDYLRKVPDLEKNQKIKDLATKMASGNLSTIA 119
SE+ E L KRK+ D RV++ E V+ YL KVP+LE+ Q + +L + +++ +++
Sbjct: 60 FSERKELSLDKRKLSDSQARVSDVEEQVNQYLSKVPELEQKQNVSELLSLLSNSPNISLS 119

Query: 120 QLQAYLNGFSEEKSHQYLALQAVKKFLGANPESKNLLALIDQAILTFEQNPDSWAQIDTE 179
QL+AYL G SEE S Q+ L ++ L PE +L L++QA+++ + +
Sbjct: 120 QLKAYLEGKSEEPSEQFKMLCGLRDALKGRPELAHLSHLVEQALVSMAEEQGETIVLGAR 179

Query: 180 IRVSSFADEYSQEQGFSSLHQLRGFYRDTVHSYQGLGSAYKDVVERFGAKEVSTAVDFML 239
I ++ + S G + L LR YRD V YQG+ + + D+ +RF ++ + + F+
Sbjct: 180 ITPEAYRESQS---GVNPLQPLRDTYRDAVMGYQGIYAIWSDLQKRFPNGDIDSVILFLQ 236

Query: 240 QGMSADLSVQGSNIDSVKLQLLMSDMQKLKTLNTLQDQVSNLYQMFKPQQAN 291
+ +SADL Q S KL +++SD+QKLK ++ DQV +Q F + N
Sbjct: 237 KALSADLQSQQSGSGREKLGIVISDLQKLKEFGSVSDQVKGFWQFFSEGKTN 288


49VIBHAR_01871VIBHAR_01877N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_01871114-0.012897hypothetical protein
VIBHAR_01872-2100.148016hypothetical protein
VIBHAR_01873-2120.111490phage shock protein B
VIBHAR_01874-211-0.322115hypothetical protein
VIBHAR_01875-210-0.709015hypothetical protein
VIBHAR_01876-211-0.763898hypothetical protein
VIBHAR_01877-111-0.607599hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01871HTHFIS355e-122 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 355 bits (912), Expect = e-122
Identities = 126/341 (36%), Positives = 190/341 (55%), Gaps = 13/341 (3%)

Query: 24 QNLIGESPAFLAVLDKVSQLAPIERPVLIIGERGTGKELIAQRLHYLSKRWDKPLLSLNC 83
L+G S A + +++L + ++I GE GTGKEL+A+ LH KR + P +++N
Sbjct: 137 MPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINM 196

Query: 84 ATLSEGLIDSELFGHESGSFTGSKGKHKGRFERAEGGTLFLDELATAPLLVQEKLLRVIE 143
A + LI+SELFGHE G+FTG++ + GRFE+AEGGTLFLDE+ P+ Q +LLRV++
Sbjct: 197 AAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQ 256

Query: 144 YGEYERVGGHTALTADVRLVCATNADLPKLAEQGDFRADLLDRLAFDVIMLPPLRERKED 203
GEY VGG T + +DVR+V ATN DL + QG FR DL RL + LPPLR+R ED
Sbjct: 257 QGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAED 316

Query: 204 ILSLAEHYAIKMCRELQLEYFVGFTEEAQQSLLDYEWPGNVRELKNVIERAIYRHGLNSD 263
I L H+ + +E F +EA + + + WPGNVREL+N++ R + +
Sbjct: 317 IPDLVRHFVQQAEKEGLDV--KRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVI 374

Query: 264 PIEHLIFNPFQTGWDNSLGHTAAQEETET-----------TEATPHSAIHFPLDYKQWQE 312
E + D+ + AA+ + + A+ A+ Y +
Sbjct: 375 TREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLA 434

Query: 313 DQDIELLNRALEEAKFNQRQAAELLGLSYHQLRGMVRKYGL 353
+ + L+ AL + NQ +AA+LLGL+ + LR +R+ G+
Sbjct: 435 EMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGV 475


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01873FLGHOOKAP1260.017 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 26.1 bits (57), Expect = 0.017
Identities = 7/29 (24%), Positives = 17/29 (58%)

Query: 37 SSQDLERLQVLSEKAEAMQSRVETLERIL 65
+++D Q L K+E + ++ +T ++ L
Sbjct: 122 NAEDPAARQALIGKSEGLVNQFKTTDQYL 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01875RTXTOXIND462e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 45.6 bits (108), Expect = 2e-07
Identities = 30/205 (14%), Positives = 66/205 (32%), Gaps = 31/205 (15%)

Query: 69 QSIEVNAGQMVKKGDVLAVLNPDEYSLLEKQARANYTLADVQYERSKKLRKEQVVSEQDF 128
+ +E V K + + E +L + + E KLR+
Sbjct: 260 KYVEAVNELRVYKSQLEQI----ESEILSAKEEYQLVTQLFKNEILDKLRQ--------- 306

Query: 129 DAAKANHNSAKAQWNQAKANLRYTKLVAPYDGTISYLPAENH-EYVAAKEGVMNI-QTNQ 186
N + + + + + + AP + L V E +M I +
Sbjct: 307 --TTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDD 364

Query: 187 LMKVLFQLPDHLLSKFTQGANAYATMTFDAFPEQSF-----ELTFQEIDTEADPKTG-SY 240
++V + + + G N A + +AFP + ++ +D D + G +
Sbjct: 365 TLEVTALVQNKDIGFINVGQN--AIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVF 422

Query: 241 KVTM------VMARPEGVGILPGMS 259
V + + + + + GM+
Sbjct: 423 NVIISIEENCLSTGNKNIPLSSGMA 447



Score = 36.3 bits (84), Expect = 2e-04
Identities = 20/114 (17%), Positives = 39/114 (34%), Gaps = 17/114 (14%)

Query: 54 AGDRAVLAFRVPGLLQSIEVNAGQMVKKGDVLAVLNPDEYSLLEKQARANYTLADVQYER 113
+G + +++ I V G+ V+KGDVL L + +++ A ++ R
Sbjct: 93 SGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTR 152

Query: 114 SKKLRKE-----------------QVVSEQDFDAAKANHNSAKAQWNQAKANLR 150
+ L + Q VSE++ + + W K
Sbjct: 153 YQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKE 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01876RTXTOXIND492e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 48.7 bits (116), Expect = 2e-08
Identities = 35/181 (19%), Positives = 63/181 (34%), Gaps = 37/181 (20%)

Query: 67 GEVSRVLVKEGDKVVKGDVIATLDPTDYQLDVDNASARFSVVD-SQYRRSA--------- 116
V ++VKEG+ V KGDV+ L + D + Q R
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNK 164

Query: 117 ------------------PLVKKGLLAKSQFDEFAAQRQIALAELELAKLRLSFTELKAP 158
+++ L K QF Q Q EL L K R + A
Sbjct: 165 LPELKLPDEPYFQNVSEEEVLRLTSLIKEQF--STWQNQKYQKELNLDKKRAERLTVLAR 222

Query: 159 VDGIISRVNVDQFENIQVGQYIVNIHSVDRVEVL------IQLPDRLHVNQSPKEELLSS 212
++ + V++ + +++ ++ + VL ++ + L V +S E++ S
Sbjct: 223 INRYENLSRVEK-SRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESE 281

Query: 213 I 213
I
Sbjct: 282 I 282



Score = 34.8 bits (80), Expect = 5e-04
Identities = 19/79 (24%), Positives = 34/79 (43%), Gaps = 8/79 (10%)

Query: 134 AQRQIALAELELAKL--RLSFTELKAPVDGIISRVNV-DQFENIQVGQYIVNIHSVDR-- 188
I L LELAK R + ++APV + ++ V + + + ++ I D
Sbjct: 307 TTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTL 366

Query: 189 -VEVLIQ--LPDRLHVNQS 204
V L+Q ++V Q+
Sbjct: 367 EVTALVQNKDIGFINVGQN 385


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_01877ACRIFLAVINRP506e-165 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 506 bits (1305), Expect = e-165
Identities = 226/1053 (21%), Positives = 444/1053 (42%), Gaps = 64/1053 (6%)

Query: 20 IAAYFIHNRVISWMVSLIFLIGGVSAFFGLGRLEDPAFTIKDAMVVTSYPGATPQQVEEE 79
+A +FI + +W++++I ++ G A L + P V +YPGA Q V++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 80 VTYPLEKAIQQLTYVDEVNSISSR-GLSQITVTMKNNYGPDDLPQIWDELRRKVNDLKGS 138
VT +E+ + + + ++S S G IT+T ++ PD +++ K+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQV---QVQNKLQLATPL 117

Query: 139 LPPGVNEPSV-IDDFGDVYGILLAVTGDGYSY--KELLDYVD-YLRRELELVDGVSKVSV 194
LP V + + ++ Y ++ D ++ DYV ++ L ++GV V +
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 195 SGQQQEQVFIEISMKKLSSLGLSPNTVFNLLSTQNVVSDAGAIRIGDEYI-------RIQ 247
G Q + I + L+ L+P V N L QN AG + G + I
Sbjct: 178 FGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQL-GGTPALPGQQLNASII 235

Query: 248 PTGEFQSVDELGDLLLTESGAQGLIFLKDVAEIKRGYVDVPTNIINYNGQLALNLGVSFA 307
F++ +E G + L + ++ LKDVA ++ G + I NG+ A LG+ A
Sbjct: 236 AQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELG-GENYNVIARINGKPAAGLGIKLA 294

Query: 308 QGVNVVKVGKAFDRRVAELKYQQPVGIQISEIYNQPKEVDKSVSGFVISLAQAVGIVIVV 367
G N + KA ++AEL+ P G+++ Y+ V S+ V +L +A+ +V +V
Sbjct: 295 TGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLV 354

Query: 368 LLFFMG-LRSGLLIGLILLLTVLGTFIFMKYLAIDLQRISLGALVIALGMLVDNAIVVVE 426
+ F+ +R+ L+ + + + +LGTF + + +++ +V+A+G+LVD+AIVVVE
Sbjct: 355 MYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVE 414

Query: 427 GILIGTQKGRTRLQAAT-DIVTQTKWPLLGATVIAVTAFAPIGLSEDSTGEYCGTLFTVL 485
+ + + + AT ++Q + L+G ++ F P+ STG +
Sbjct: 415 NVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITI 474

Query: 486 LISLMLSWFTAISLTPFFADIFFKGQKIKSGEGED------NDPYNGIVFVMYKKFLSFC 539
+ ++ LS A+ LTP K + E + N ++ V Y +
Sbjct: 475 VSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVN-HYTNSVGKI 533

Query: 540 MRRAWFTVVVLVAGLAASVYGFTYVKQAFFPSSTTPIFQLDVWLPEGTDIRATNDTLKEL 599
+ +++ +A V F + +F P +F + LP G T L ++
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 600 EGWL--AEQDHVDHITTTAGKGLQRFMLTYAPEKSYAAYGEIT-----TRVENYEALAPL 652
+ E+ +V+ + T G +++ + A ++ R + + +
Sbjct: 594 TDYYLKNEKANVESVFTVNG-------FSFSGQAQNAGMAFVSLKPWEERNGDENSAEAV 646

Query: 653 MDRFREHIKTNYPEINYKLK---QIELGPGGGAKIE-ARIIGSDPTVLRTIAAQVMDVMY 708
+ R + + +ELG G E G L Q++ +
Sbjct: 647 IHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAA 706

Query: 709 ADPGA-TNIRHDWRERTQVLEPQFNESQARRYGITKSDVDDFLSMSFSGMTIGLYRDGTT 767
P + ++R + E T + + ++ +A+ G++ SD++ +S + G + + D
Sbjct: 707 QHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGR 766

Query: 768 LMPIVARLPEDERIDIRNIEGMKIWSPAQSEFIPLQQVTMGYDMRWED--PIIVRKNRKR 825
+ + + R+ +++ + + S A E +P T W P + R N
Sbjct: 767 VKKLYVQADAKFRMLPEDVDKLYVRS-ANGEMVPFSAFT---TSHWVYGSPRLERYNGLP 822

Query: 826 MLTVMADPD-ILGEETAATLQKRLQPAIEAIEMPPGYSLEWGGEYESSGKAKASLFSTMP 884
+ + + A L + L A ++P G +W G + + +
Sbjct: 823 SMEIQGEAAPGTSSGDAMALMENL-----ASKLPAGIGYDWTGMSYQERLSGNQAPALVA 877

Query: 885 MGYLFMFLITIFLFNSIKEPLIVWLTVPLALIGVTTGLLALNTPFGFMALLGFLSLSGMV 944
+ ++ +FL L+ S P+ V L VPL ++GV N ++G L+ G+
Sbjct: 878 ISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLS 937

Query: 945 LKNGIVLLDQI-EIEMKSGKEAYDAVVDAAVSRVRPVCMAAITTILGMIPLLPDI----- 998
KN I++++ ++ K GK +A + A R+RP+ M ++ ILG++PL
Sbjct: 938 AKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSG 997

Query: 999 FFKPMAVTIMFGLGFATVLTLIVVPVLYRLFHR 1031
+ + +M G+ AT+L + VPV + + R
Sbjct: 998 AQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR 1030


50VIBHAR_02191VIBHAR_02197N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02191118-1.341012hypothetical protein
VIBHAR_02193215-1.192004hypothetical protein
VIBHAR_02194219-0.942127hypothetical protein
VIBHAR_02195016-0.501247chitinase
VIBHAR_02196017-0.294996hypothetical protein
VIBHAR_02197118-0.818684hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02191SACTRNSFRASE388e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.0 bits (88), Expect = 8e-06
Identities = 14/47 (29%), Positives = 21/47 (44%), Gaps = 5/47 (10%)

Query: 135 RGKGIGKMLLQHAI-----KHLGAVKVDVNEQNPQAVGFYQRMGFKV 176
R KG+G LL AI H + ++ + N A FY + F +
Sbjct: 102 RKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02194MICOLLPTASE310.030 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 30.8 bits (69), Expect = 0.030
Identities = 13/42 (30%), Positives = 18/42 (42%)

Query: 689 SENNKPVAHAGEDQSVLSGATITLSADKSTDADGDKLTYVWE 730
N +P A D SV+ I +S D DG+ Y W+
Sbjct: 770 HVNKEPKAVIKSDSSVIVEEEINFDGTESKDEDGEIKAYEWD 811


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02195MICOLLPTASE343e-04 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 33.9 bits (77), Expect = 3e-04
Identities = 22/101 (21%), Positives = 38/101 (37%), Gaps = 14/101 (13%)

Query: 27 NQPPIAVATSNLYELTGAGSISLSGAQSRDPNGDLLTFEWKQLSPSSPLADIASPTTEET 86
N+ P AV S+ + I+ G +S+D +G++ +EW D
Sbjct: 772 NKEPKAVIKSD-SSVIVEEEINFDGTESKDEDGEIKAYEW----------DFGDGEKSNE 820

Query: 87 TVRFAEIAADTTYRFLLTVKD---GSLFDTSEVVVVQKAKV 124
+ Y LTV D G ++ ++ VV+ V
Sbjct: 821 AKATHKYNKTGEYEVKLTVTDNNGGINTESKKIKVVEDKPV 861


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02197TYPE3IMSPROT310.009 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 31.3 bits (71), Expect = 0.009
Identities = 25/180 (13%), Positives = 67/180 (37%), Gaps = 19/180 (10%)

Query: 90 ELTTVFILASLTAAAVSY--LIVHHLIVRSVNNVLQKANKPLAESLAKYDVLEKLSLLIP 147
E+ + ++ +L+A + H + + +++ P +++L+ ++ + L
Sbjct: 28 EVVSTALIVALSAMLMGLSDYYFEHFS-KLMLIPAEQSYLPFSQALSYV--VDNVLLEFF 84

Query: 148 IMVLDVFIPLAVGHYDWLAAIFDRILSISILVIFVWMIFALLDAMNDIATFKGITKKMPI 207
+ + A I S + F+ A+ + I +G + I
Sbjct: 85 YLCFPLLT----------VAALMAIASHVVQYGFLISGEAIKPDIKKINPIEGAKRIFSI 134

Query: 208 KSFVQLIK--LFTFFVGVVVSMSILANESPVIFLSGLGVATGF--VMLVFKDTILGFVAG 263
KS V+ +K L + +++ + I N ++ L G+ + + + ++ G
Sbjct: 135 KSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQILRQLMVICTVG 194


51VIBHAR_02519VIBHAR_02525N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02519-3132.093542molecular chaperone
VIBHAR_02520-2141.187604hypothetical protein
VIBHAR_02521015-0.557892hypothetical protein
VIBHAR_02522-115-0.4958843-methyladenine DNA glycosylase
VIBHAR_02523-216-0.762957hypothetical protein
VIBHAR_02524-212-0.296093hypothetical protein
VIBHAR_02525-114-1.002994hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02519SHAPEPROTEIN531e-09 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 53.2 bits (128), Expect = 1e-09
Identities = 34/150 (22%), Positives = 59/150 (39%), Gaps = 23/150 (15%)

Query: 136 SPEEVEKVSPLRA-----TELYLEHLKNAWNHTKPNHNLADQD-VTITVPASFDPAARDL 189
+P + + P++ + + L++ N + V + VP R
Sbjct: 66 TPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRA 125

Query: 190 TAEAARNVGFVHLTLLEEPQAALYNWIDNSNDKWRDEVEVGDIVLVVDIGGGTTDLSLVE 249
E+A+ G + L+EEP AA I +VVDIGGGTT+++++
Sbjct: 126 IRESAQGAGAREVFLIEEPMAAA---IGAGLPVSEATGS-----MVVDIGGGTTEVAVIS 177

Query: 250 VTEDDGNLTLNRIAVGEHILLGGDNMDLAL 279
LN + + +GGD D A+
Sbjct: 178 ---------LNGVVYSSSVRIGGDRFDEAI 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02520SHAPEPROTEIN562e-10 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 56.3 bits (136), Expect = 2e-10
Identities = 44/181 (24%), Positives = 74/181 (40%), Gaps = 36/181 (19%)

Query: 165 VVVTVPASFDETARKLTLEAAELAGLKKIVLLEEPQAVCYDWYARHQQTAAD-ELKELPL 223
V+V VP + R+ E+A+ AG +++ L+EEP A A + E
Sbjct: 110 VLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAA---------IGAGLPVSEATG 160

Query: 224 ILVCDVGGGTTDLSLIEASFSSQNELALDRIGVGEHLMLGGDNLDLALAHLAESRLSQNK 283
+V D+GGGTT++++I L+ + + +GGD D A+ +
Sbjct: 161 SMVVDIGGGTTEVAVIS----------LNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLI 210

Query: 284 GEQSKKLTAASLTKLIQQTRKAKENLLSASAPEEVKITMLGSGSKLL-GGTKSIGLSKQE 342
GE TA + K + SA +EV+ + G L G + L+ E
Sbjct: 211 GEA----TA----------ERIKHEIGSAYPGDEVR-EIEVRGRNLAEGVPRGFTLNSNE 255

Query: 343 V 343
+
Sbjct: 256 I 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02523BCTERIALGSPG412e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 41.4 bits (97), Expect = 2e-07
Identities = 17/52 (32%), Positives = 29/52 (55%)

Query: 1 MRNTKGFTLIELVVCIVILGVVSVVALPKFLGIQKDARIAVLYGAREALMTA 52
+GFTL+E++V IVI+GV++ + +P +G ++ A AL A
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENA 55


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02525ACRIFLAVINRP330.004 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 32.9 bits (75), Expect = 0.004
Identities = 13/62 (20%), Positives = 30/62 (48%), Gaps = 3/62 (4%)

Query: 134 FYELLIATVVKN---AVMILVMLGVMFYMSWKLTIVVLVLLPIVIGFMYLFKKLSTESYR 190
F +L I VVK A+M++ ++ +F + + T++ + +P+V+ + S
Sbjct: 332 FVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSIN 391

Query: 191 NM 192
+
Sbjct: 392 TL 393


52VIBHAR_02541VIBHAR_02547N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02541018-0.374058hypothetical protein
VIBHAR_02542019-0.697640hypothetical protein
VIBHAR_02544-121-1.840861hypothetical protein
VIBHAR_02543-122-1.808868hypothetical protein
VIBHAR_02545025-1.735545hypothetical protein
VIBHAR_02546-122-1.243405hypothetical protein
VIBHAR_02547020-0.808797hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02541BCTERIALGSPF270.010 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 26.7 bits (59), Expect = 0.010
Identities = 8/56 (14%), Positives = 18/56 (32%), Gaps = 1/56 (1%)

Query: 3 FTALFPQFIDPSASLFTQYIVFASTFVVFELSWLTFYGLLGVKTSDWLFVKGRAKL 58
F + L T+ ++ S V W+ L G + + + ++
Sbjct: 198 VVEQFIHM-KQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMAFRVMLRQEKRRV 252


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02545HTHFIS544e-11 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 53.7 bits (129), Expect = 4e-11
Identities = 27/125 (21%), Positives = 44/125 (35%), Gaps = 16/125 (12%)

Query: 27 LICVDDQ---REVLSAVLQDLQLLSQWLNIEDCESADEVLELMDELDAEGEMIALIISDH 83
++ DD R VL+ L D L + A L+++D
Sbjct: 6 ILVADDDAAIRTVLNQALSRAG--------YDVRITSNAATLWRWIAAGD--GDLVVTDV 55

Query: 84 VMPGKTGVELLTEISKDSRFIRARKVLLTGQTTHTDTINAINSAGIDRYFEKPWQAEQLV 143
VMP + +LL I K ++++ Q T I A G Y KP+ +L+
Sbjct: 56 VMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASE-KGAYDYLPKPFDLTELI 112

Query: 144 ECVRN 148
+
Sbjct: 113 GIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02546SACTRNSFRASE413e-07 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 41.1 bits (96), Expect = 3e-07
Identities = 16/81 (19%), Positives = 36/81 (44%), Gaps = 7/81 (8%)

Query: 59 FVAELDGVIVGYSDVQPS----GLVDHFFCHHEYQGQGVGRVLMTHVLKQAEAKGLNRID 114
F+ L+ +G ++ + L++ +Y+ +GVG L+ ++ A+ +
Sbjct: 68 FLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLM 127

Query: 115 SE---VSITARPFYEHMGFTV 132
E ++I+A FY F +
Sbjct: 128 LETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02547SACTRNSFRASE300.005 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 29.5 bits (66), Expect = 0.005
Identities = 13/80 (16%), Positives = 30/80 (37%), Gaps = 5/80 (6%)

Query: 75 DNQIVGQLECRYPVLDEEGRKFGYINLFYLLPEFRNQGLGRQLQDFLLKLFANHGCRYAR 134
+N +G+++ R + I + ++R +G+G L ++ +
Sbjct: 73 ENNCIGRIKIR-----SNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLM 127

Query: 135 LRYIPGNDAGERFYYKNGWR 154
L N + FY K+ +
Sbjct: 128 LETQDINISACHFYAKHHFI 147


53VIBHAR_02658VIBHAR_02664N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_02658-115-0.415663hypothetical protein
VIBHAR_02659-117-1.491938hypothetical protein
VIBHAR_02660-111-1.823929hypothetical protein
VIBHAR_02661115-1.979078glycosyltransferase
VIBHAR_02662219-1.54065050S ribosomal protein L25
VIBHAR_02663118-2.020929acetyltransferase
VIBHAR_02664121-2.351161hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02658HTHFIS817e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 80.6 bits (199), Expect = 7e-20
Identities = 29/124 (23%), Positives = 57/124 (45%)

Query: 2 KILVVEDEPRLGEQILETLENNGWVPELSQDGIDALYRATSEEWDAIVLDLGLPKLDGLT 61
ILV +D+ + + + L G+ ++ + + + D +V D+ +P +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLKGIRDENINTPVVILSARDTLSQRVEGLNAGADDYLTKPFEMVELTARLRAQLRRASG 121
+L I+ + PV+++SA++T ++ GA DYL KPF++ EL + L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 122 NASP 125
S
Sbjct: 125 RPSK 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02659PF06580355e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 35.2 bits (81), Expect = 5e-04
Identities = 17/82 (20%), Positives = 30/82 (36%), Gaps = 16/82 (19%)

Query: 365 IIRVHSTLDKDNIHIIVEDDGPGIPAEQLCQVIKRGYRLDETTPGSGLGLNIVSE-MAHS 423
I + T D + + VE+ G L T +G GL V E +
Sbjct: 280 KILLKGTKDNGTVTLEVENTGSLA--------------LKNTKESTGTGLQNVRERLQML 325

Query: 424 YRGQLELS-ESKMGGLKATLIL 444
Y + ++ K G + A +++
Sbjct: 326 YGTEAQIKLSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02663SACTRNSFRASE357e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 34.5 bits (79), Expect = 7e-05
Identities = 13/54 (24%), Positives = 21/54 (38%)

Query: 94 VSPLYFGQGAGSALFDELETRLLSTPVMQISLKALDTNKQGLNFYKKCGFVRSG 147
V+ Y +G G+AL + + L+ D N +FY K F+
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_02664SACTRNSFRASE280.023 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.0 bits (62), Expect = 0.023
Identities = 12/52 (23%), Positives = 16/52 (30%), Gaps = 1/52 (1%)

Query: 172 ILVAPEARGKGYGKRLLAHLVSEVKSRQR-KVRYRVNIENTPSIKLCQSLGF 222
I VA + R KG G LL + K + N + F
Sbjct: 95 IAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


54VIBHAR_03138VIBHAR_03179N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_031381132.759404hypothetical protein
VIBHAR_031391152.218495hypothetical protein
VIBHAR_031401152.047670chemotaxis-specific methylesterase
VIBHAR_031411121.761976hypothetical protein
VIBHAR_03142-1151.148476hypothetical protein
VIBHAR_03143-2151.589785hypothetical protein
VIBHAR_03144-2172.066872flagellar biosynthesis sigma factor
VIBHAR_03145-1182.366879hypothetical protein
VIBHAR_031460222.648393flagellar biosynthesis regulator FlhF
VIBHAR_031471232.393351flagellar biosynthesis protein FlhA
VIBHAR_031482242.315053flagellar biosynthesis protein FlhB
VIBHAR_031493222.443199flagellar biosynthesis protein FliR
VIBHAR_031504212.316374flagellar biosynthesis protein FliQ
VIBHAR_031512162.386444flagellar biosynthesis protein FliP
VIBHAR_031521152.036572hypothetical protein
VIBHAR_031531152.004967flagellar motor switch protein
VIBHAR_031540162.543319flagellar motor switch protein FliM
VIBHAR_031551172.363828flagellar basal body-associated protein FliL
VIBHAR_031560132.626307hypothetical protein
VIBHAR_03157-1173.060994hypothetical protein
VIBHAR_031580183.298516flagellar biosynthesis chaperone
VIBHAR_03159-1173.472771flagellum-specific ATP synthase
VIBHAR_03160-1152.630759flagellar assembly protein H
VIBHAR_03161-1152.515634flagellar motor switch protein G
VIBHAR_03162-1152.062164flagellar MS-ring protein
VIBHAR_03163-2141.059272flagellar hook-basal body protein FliE
VIBHAR_03164-2112.020639hypothetical protein
VIBHAR_03165-1111.779371hypothetical protein
VIBHAR_031660131.540651hypothetical protein
VIBHAR_03167190.529165flagellar protein FliS
VIBHAR_031682130.657389hypothetical protein
VIBHAR_031691121.099599flagellar capping protein
VIBHAR_03170116-0.183398flagellar protein FlaG
VIBHAR_03171115-0.702821flagellin
VIBHAR_03172013-0.379124hypothetical protein
VIBHAR_03173-114-0.061950flagellin
VIBHAR_03174-211-0.325799flagellin
VIBHAR_03175-213-0.810213hypothetical protein
VIBHAR_03176-214-0.681787hypothetical protein
VIBHAR_03177-216-0.290989hypothetical protein
VIBHAR_031780171.243944hypothetical protein
VIBHAR_031791161.517957hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03138TONBPROTEIN594e-12 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 58.8 bits (142), Expect = 4e-12
Identities = 27/76 (35%), Positives = 40/76 (52%), Gaps = 4/76 (5%)

Query: 26 EVIEQEEELDLLAQELSSAEPEPEPEPEPEPEP---EPEPEPEPEPEPEPE-PEPEPEPE 81
+VIE ++ + + P+ P EPEPEPEP PEP E P +P+
Sbjct: 34 QVIELPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPK 93

Query: 82 PEPEPEPEPEPKLQLQ 97
P+P+P+P+P K+Q Q
Sbjct: 94 PKPKPKPKPVKKVQEQ 109



Score = 47.7 bits (113), Expect = 2e-08
Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 5/68 (7%)

Query: 31 EEELDLLAQELSSAEPEPEPEPEPEPEPE-----PEPEPEPEPEPEPEPEPEPEPEPEPE 85
E + EPEPEPEP PEP E +P+P+P+P+P+P + + +P+ + +
Sbjct: 56 EPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVK 115

Query: 86 PEPEPEPK 93
P
Sbjct: 116 PVESRPAS 123



Score = 37.3 bits (86), Expect = 5e-05
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 1/41 (2%)

Query: 62 PEPEPEPEPEPEPEPEPEPEPEPEPEPEPEPKLQLQYAEPE 102
P P+ P P EPEPEPEP PEP + +
Sbjct: 52 PADLEPPQAVQPPPE-PVVEPEPEPEPIPEPPKEAPVVIEK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03140HTHFIS664e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.4 bits (162), Expect = 4e-14
Identities = 28/166 (16%), Positives = 60/166 (36%), Gaps = 6/166 (3%)

Query: 2 AIKVLVVDDSSFFRRRVSEIINSESRLEVIDVAVNGREAVEKAKTLKPDVITMDIEMPVM 61
+LV DD + R +++ ++ + + N D++ D+ MP
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 62 DGITAVREIMAASP-TPILMFSSLTHDGAKATLDALDAGALDFLPKKFEDIARNRDEAVS 120
+ + I A P P+L+ S+ + + A + GA D+LPK F D+
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSA--QNTFMTAIKASEKGAYDYLPKPF-DLTELIGIIGR 117

Query: 121 LLQQRVIQIASKRAFMRRPIARPAAAATSSTARPLTSRTATPTASV 166
L + + + + + +A + +R ++
Sbjct: 118 ALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTL 163


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03141PF06580441e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 44.1 bits (104), Expect = 1e-06
Identities = 18/104 (17%), Positives = 41/104 (39%), Gaps = 18/104 (17%)

Query: 449 ETDLDKNLVEALADPLI--HLVRNSVDHGIEMPDDRAKAGKSRTGKVVLSASQEGDHIEL 506
E ++ +++ P++ LV N + HGI + GK++L +++ + L
Sbjct: 243 ENQINPAIMDVQVPPMLVQTLVENGIKHGIA--------QLPQGGKILLKGTKDNGTVTL 294

Query: 507 AIVDDGGGMDPNKLRGIAVK--------RGMMDEDAAARLSDKE 542
+ + G N + + +A +LS+K+
Sbjct: 295 EVENTGSLALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQ 338


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03143HTHFIS903e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.9 bits (223), Expect = 3e-24
Identities = 33/105 (31%), Positives = 51/105 (48%), Gaps = 3/105 (2%)

Query: 10 KILIVDDFSTMRRIVKNLLRDLGFNNTQEADDGLTALPMLKKGDFDFVVTDWNMPGMQGI 69
IL+ DD + +R ++ L G++ + T + GD D VVTD MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 70 DLLKHIRADAELKHLPVLMITAEAKREQIIEAAQAGVNGYIVKPF 114
DLL I+ LPVL+++A+ I+A++ G Y+ KPF
Sbjct: 64 DLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03148TYPE3IMSPROT359e-125 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 359 bits (922), Expect = e-125
Identities = 111/354 (31%), Positives = 189/354 (53%), Gaps = 14/354 (3%)

Query: 8 ERTEEATPRRLQQAREKGQVARSKELASASVLIVGAIALMWFGESLARSLFSIMSRLFDL 67
E+TE+ TP++++ AR+KGQVA+SKE+ S ++++ + LM L+ F S+L +
Sbjct: 4 EKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLM----GLSDYYFEHFSKLMLI 59

Query: 68 TRDEIFDTAKLFDIAFGAMTDLLFPLFLI--LITLFVAAMIGAAG---VGGISFSAEAAM 122
++ + F A + D + F L VAA++ A G S EA
Sbjct: 60 PAEQSYLP---FSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIK 116

Query: 123 PKLSKMNPLSGLKRMVGMQSWVELIKSILKVALVTGMAIYLIQASQADLIQLSMDVYPQN 182
P + K+NP+ G KR+ ++S VE +KSILKV L++ + +I+ + L+QL +
Sbjct: 117 PDIKKINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQL-PTCGIEC 175

Query: 183 IFHAL-DILLNFILLISCSLLIVVAIDIPFQIWQHADQLKMTKQEVKDEYKETEGKPEVK 241
I L IL +++ + +++ D F+ +Q+ +LKM+K E+K EYKE EG PE+K
Sbjct: 176 ITPLLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIK 235

Query: 242 GRIRMLQREAAQRRMMADVPQADVIITNPEHFSVALRYKQKTDRAPVVIAKGTDHMAMKI 301
+ R +E R M +V ++ V++ NP H ++ + YK+ P+V K TD +
Sbjct: 236 SKRRQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTV 295

Query: 302 REVAREHDITIVPAPPLARALYHTTELEQEIPDGLFTAVAQVLAFVFQLKQYRK 355
R++A E + I+ PLARALY ++ IP A A+VL ++ + ++
Sbjct: 296 RKIAEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNIEKQ 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03149TYPE3IMRPROT1277e-38 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 127 bits (321), Expect = 7e-38
Identities = 87/221 (39%), Positives = 131/221 (59%), Gaps = 2/221 (0%)

Query: 9 LDWIANYFWPYTRISAMLMVMTVTGARFVSPRIRLYLGLAITFAVAPAIPAVPQDIELLS 68
L W+ YFWP R+ A++ + R V R++L L + ITFA+AP++PA D+ + S
Sbjct: 10 LSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA--NDVPVFS 67

Query: 69 FRGFMTIAEQIVIGVAMGMVTQFMIQTFVLLGQILGMQSSLGFASMVDPANGQNTPLLGQ 128
F +QI+IG+A+G QF G+I+G+Q L FA+ VDPA+ N P+L +
Sbjct: 68 FFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLAR 127

Query: 129 LFMFLATMFFLATDGHLKMLQLVVFSFKTLPIGSGSLNAVDFREMASWLGIMFKTALSMS 188
+ LA + FL +GHL ++ L+V +F TLPIG LN+ F + ++F L ++
Sbjct: 128 IMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLA 187

Query: 189 LSGIIALLTINLSFGVMTRAAPQLNIFSLGFAFALMVGLLL 229
L I LLT+NL+ G++ R APQL+IF +GF L VG+ L
Sbjct: 188 LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISL 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03150TYPE3IMQPROT541e-13 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 54.4 bits (131), Expect = 1e-13
Identities = 26/70 (37%), Positives = 39/70 (55%)

Query: 7 VELFRDALWMVLIMVCAIIIPSLLIGLVVAIFQAATSINEQTLSFLPRLIVTLLALMLFG 66
V AL++VLI+ I + +IGL+V +FQ T + EQTL F +L+ L L L
Sbjct: 5 VFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFLLS 64

Query: 67 HWMTQMLMEY 76
W ++L+ Y
Sbjct: 65 GWYGEVLLSY 74


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03151FLGBIOSNFLIP2847e-99 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 284 bits (728), Expect = 7e-99
Identities = 113/229 (49%), Positives = 165/229 (72%), Gaps = 1/229 (0%)

Query: 60 MSVGNGGGIPAFTMTTNADGSEDYSVTLQILALMTMLGFLPAMVILMTSFTRIVVVMSIL 119
++ +P T G + +S+ +Q L +T L F+PA++++MTSFTRI++V +L
Sbjct: 15 ITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVFGLL 74

Query: 120 RQAMGLQQTPSNQVIIGIALFLTFFVMSPVLNEINDNAIQPYLNEQVTARQAFDAAQVPM 179
R A+G P NQV++G+ALFLTFF+MSPV+++I +A QP+ E+++ ++A + P+
Sbjct: 75 RNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGAQPL 134

Query: 180 KAFMLKQTRIKDLETFVNMSGE-QVTNPEDVSMAVLIPAFITSELKTAFQIGFMLFLPFL 238
+ FML+QTR DL F ++ + PE V M +L+PA++TSELKTAFQIGF +F+PFL
Sbjct: 135 REFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFL 194

Query: 239 IIDLVVASVLMAMGMMMLSPMIVSLPFKLMLFVLVDGWNLILSTLAGSF 287
IIDLV+ASVLMA+GMMM+ P ++LPFKLMLFVLVDGW L++ +LA SF
Sbjct: 195 IIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03153FLGMOTORFLIN1146e-36 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 114 bits (287), Expect = 6e-36
Identities = 63/135 (46%), Positives = 91/135 (67%), Gaps = 8/135 (5%)

Query: 3 PSDDQK--LADEWAAALGEDPSAPSIDVDEVLAAPLEELKDTSSPITDDERRKLDTIMDI 60
PSD+ L D WA AL E + + + + L D S + D +D IMDI
Sbjct: 7 PSDENTGALDDLWADALNEQKATTTKSAADAVFQQLGG-GDVSGAMQD-----IDLIMDI 60

Query: 61 PVTISMEVGRSQISIRNLLQLNQGSVVELDRLAGESLDVLVNGTLIAHGEVVVVNDKFGI 120
PV +++E+GR++++I+ LL+L QGSVV LD LAGE LD+L+NG LIA GEVVVV DK+G+
Sbjct: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120

Query: 121 RLTDVISQTERIKKL 135
R+TD+I+ +ER+++L
Sbjct: 121 RITDIITPSERMRRL 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03154FLGMOTORFLIM2482e-82 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 248 bits (634), Expect = 2e-82
Identities = 85/327 (25%), Positives = 161/327 (49%), Gaps = 9/327 (2%)

Query: 1 MTDLLSQDEIDALLHGVD--DVDDVDEPMDAGDESAVSFDFSSQDRIVRGRMPTLELINE 58
MT++LSQDEID LL + D D + +DF D+ + +M TL L++E
Sbjct: 1 MTEVLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHE 60

Query: 59 RFARHMRISLFNMLRKTAEVSINGVQMMKFGEYQNTLYVPTSLNMVRFRPLKGTALITME 118
FAR SL LR V + V + + E+ ++ P++L ++ PLKG A++ ++
Sbjct: 61 TFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVD 120

Query: 119 ARLVFILVENFFGGDGRFHAKIEGREFTPTERRIIQLLLKIVFEDYKEAWSPVMGVEFEY 178
+ F +++ FGG G+ R+ T E +++ ++ + + +E+W+ V+ +
Sbjct: 121 PSITFSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRL 178

Query: 179 LDSEVNPSMANIVSPTEVIVVSSFHIEVDGGGGDFHVVMPYSMVEPIRELLDAG--VQSD 236
E NP A IV P+E++V+ + +V G + +PY +EPI L + S
Sbjct: 179 GQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSV 238

Query: 237 KMETDVRWSSALREEIMDCPVNFRVNLLEKDISLRDLMELQPGDVIPIE---MPEHATMF 293
+ + ++ LR+++ ++ + +S+RD++ L+ GD+I + + + +
Sbjct: 239 RRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLS 298

Query: 294 IEDLPTYRVKMGRSEDKMAVQISEEIE 320
I + + + G K+A QI E IE
Sbjct: 299 IGNRKKFLCQPGVVGKKIAAQILERIE 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03155PilS_PF08805270.036 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 26.8 bits (59), Expect = 0.036
Identities = 10/49 (20%), Positives = 22/49 (44%), Gaps = 2/49 (4%)

Query: 13 KGKSKLLIIIIAVVVLLLGGGSAAFFLMGSDEPAQAAQAEQTQAQTAAA 61
KG + + ++++ V+++L A+ L + + EQ T A
Sbjct: 26 KGATLMEVLLVVGVIVVLAAS--AYKLYSMVQSNIQSSNEQNNVLTVIA 72


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03156FLGHOOKFLIK465e-07 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 45.6 bits (107), Expect = 5e-07
Identities = 27/117 (23%), Positives = 60/117 (51%)

Query: 555 AEKVQMMMSKNLKNLDIRLDPPELGRMQIRMTMNNDLANVHFTVANPQARDLIEQTLPRL 614
++ + + + ++ ++RL P +LG +QI + ++++ A + + R +E LP L
Sbjct: 245 SQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVL 304

Query: 615 REMLAQQGMQLADSSVQQQSSGQQQSGYANAEQNGQGSQGRGFSGQSDENFDADTNL 671
R LA+ G+QL S++ +S QQ + +Q+ + + +G+ D+ +L
Sbjct: 305 RTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSL 361


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03158FLGFLIJ407e-07 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 39.8 bits (92), Expect = 7e-07
Identities = 31/144 (21%), Positives = 75/144 (52%)

Query: 2 NNAMEFLLEQAKEREDQAVLALNKARSELDDYYRQVEQIEKYRLDYCQQLVDRGMAGLTA 61
+ A+ L + A++ + A L + R Q++ + Y+ +Y L AG+T+
Sbjct: 4 HGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAGITS 63

Query: 62 SQYGHLNRFLTQLDETLSKQKQAENHFKEQVVNCQDYWLNMRKERMSYEWMIEKREKEKQ 121
+++ + +F+ L++ +++ +Q N + ++V + W ++ +++ + E++
Sbjct: 64 NRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQSTAAL 123

Query: 122 MAEAKREQKQMDEFSTLLYSRKPQ 145
+AE + +QK+MDEF+ RKP+
Sbjct: 124 LAENRLDQKKMDEFAQRAAMRKPE 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03160FLGFLIH638e-14 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 62.5 bits (151), Expect = 8e-14
Identities = 45/207 (21%), Positives = 94/207 (45%), Gaps = 7/207 (3%)

Query: 48 WVPNFDESEEEQVLELTEEQIELIKQG--AYQEGLYQGQEAGFKQGYDKGKEEGIQAGHA 105
W P+ + + + + E + +I++ + ++ L Q Q +QGY G EG Q GH
Sbjct: 10 WTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHK 69

Query: 106 EGLELGKAEGVTAGQEYIQQQVET----FMDLANQFAQPLELMNAQVEKQLVDMVLCLVK 161
+G + G A+G+ G + Q L ++F L+ +++ + +L+ M L +
Sbjct: 70 QGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAAR 129

Query: 162 EVVHVEVQTNPQIILDTVKQSVEALPISGHPITLHLNPDDVAIIRSAYGEEELDCRNWTL 221
+V+ + ++ ++Q ++ P+ L ++PDD+ + G L W L
Sbjct: 130 QVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLG-ATLSLHGWRL 188

Query: 222 VAEPALNRGDVQIAAGESSISYRMEER 248
+P L+ G +++A E + + R
Sbjct: 189 RGDPTLHPGGCKVSADEGDLDASVATR 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03161FLGMOTORFLIG2913e-99 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 291 bits (746), Expect = 3e-99
Identities = 107/330 (32%), Positives = 200/330 (60%)

Query: 20 DVSSMTGEEKAAILLLSLNENDAAGIIRHLEPKQVQRVGSAMARAKDLSQDKVSGVHRAF 79
DVS++TG++KAAILL+S+ ++ + ++L ++++ + +A+ + ++ + V F
Sbjct: 11 DVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEF 70

Query: 80 LEDIQKYTNIGMGSEDFMRNALVAALGEDKANNLVDQILLGTGSKGLDSLKWMDPRQVAS 139
E + I G D+ R L +LG KA ++++ + S+ + ++ DP + +
Sbjct: 71 KELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFVRRADPANILN 130

Query: 140 IIVNEHPQIQTIVLSYLEADQSAEILSQFPERVRLDLMMRIANLEEVQPSALAELNEIME 199
I EHPQ ++LSYL+ +++ ILS P V+ ++ RIA ++ P + E+ ++E
Sbjct: 131 FIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVERVLE 190

Query: 200 KQFAGQAGAQAAKIGGLKAAAEIMNYLDNNVEGILMEQIRDQDEDMATQIQDLMFVFENL 259
K+ A + GG+ EI+N D E ++E + ++D ++A +I+ MFVFE++
Sbjct: 191 KKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMFVFEDI 250

Query: 260 VEVDDQGIQKLLRDVPQDVLQRALKGADDSLREKVFKNMSKRAAEMMRDDIEAMPPVRVA 319
V +DD+ IQ++LR++ L +ALK D ++EK+FKNMSKRAA M+++D+E + P R
Sbjct: 251 VLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLGPTRRK 310

Query: 320 DVEAAQKEILAIARRMADAGEIMLSGGADE 349
DVE +Q++I+++ R++ + GEI++S G +E
Sbjct: 311 DVEESQQKIVSLIRKLEEQGEIVISRGGEE 340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03162FLGMRINGFLIF2775e-88 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 277 bits (709), Expect = 5e-88
Identities = 146/557 (26%), Positives = 254/557 (45%), Gaps = 40/557 (7%)

Query: 47 GDLDLLRQVVLVLSISICVALIVMLFFWVKEPEMRPL-GAYETEELIPVLDYLDQQKINY 105
L ++ L+++ S VA++V + W K P+ R L ++ ++ L Q I Y
Sbjct: 17 NRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIPY 76

Query: 106 KL--DGNTISVESSDYNSIKLGMVRAGVNQATAAGDDILLQDMGFGVSQRLEQERLKLSR 163
+ I V + + ++L + + G+ + A G + LL FG+SQ EQ + +
Sbjct: 77 RFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFE-LLDQEKFGISQFSEQVNYQRAL 135

Query: 164 ERQLAQAIEEMKQVRKARVLLALPKHSVFVRHNQEASASVFLTLSTGANLKQQEVDSIVD 223
E +LA+ IE + V+ ARV LA+PK S+FVR + SASV +TL G L + ++ ++V
Sbjct: 136 EGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVH 195

Query: 224 MVASAVPGMKTSRITVTDQHGRLLSSGSQDPASAARRKEQELERSQEQALREKIDSVLLP 283
+V+SAV G+ +T+ DQ G LL+ + + + E ++ +I+++L P
Sbjct: 196 LVSSAVAGLPPGNVTLVDQSGHLLTQSN-TSGRDLNDAQLKFANDVESRIQRRIEAILSP 254

Query: 284 ILGFGNYTAQVDIQMDFSTVEQTRKNFDPNTPSTRSEYALEDYNNGNMVA-----GIPGA 338
I+G GN AQV Q+DF+ EQT +++ PN ++++ N V G+PGA
Sbjct: 255 IVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGA 314

Query: 339 LSNQPPADASIP-----------QDVAQMKDGTLTGQG---SVRKESTRNFELDTTISHE 384
LSNQP P Q+ Q T + S ++ T N+E+D TI H
Sbjct: 315 LSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHT 374

Query: 385 RKQTGTLARQTVSVAIKDRRTVNPDTGEVTYTPLSESEINAIRQVLIGTVGFDENRGDLL 444
+ G + R +V+V + + + PL+ ++ I + +GF + RGD L
Sbjct: 375 KMNVGDIERLSVAVVVNYKTLA-----DGKPLPLTADQMKQIEDLTREAMGFSDKRGDTL 429

Query: 445 NVLSVKFAEPELEQMVDPPIWEHPNFNDWVRWFASALVIIVVILVLVRPAMKKLLNPASG 504
NV++ F+ + + P W+ +F D + L+++VV +L R K + P
Sbjct: 430 NVVNSPFSAVD-NTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWR----KAVRPQLT 484

Query: 505 DEDEMYGPDGLPIGADGETSLIGSDIESGELFEFGSSIDLPN--LHKDEDVLKAVRALVA 562
E E + L + E + + +R +
Sbjct: 485 RRVEEAKAAQEQAQVRQE----TEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSD 540

Query: 563 NEPELAAQVVKNWMDEN 579
N+P + A V++ WM +
Sbjct: 541 NDPRVVALVIRQWMSND 557


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03163FLGHOOKFLIE603e-15 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 59.7 bits (144), Expect = 3e-15
Identities = 28/101 (27%), Positives = 54/101 (53%), Gaps = 3/101 (2%)

Query: 3 VDGIQAEMRAMMVEATNTAPTATGAKVGADFGDLLTKAINNVNSLQKASGDLQTRFDRGD 62
++G+ ++++A + A F L A++ ++ Q A+ +F G+
Sbjct: 6 IEGVISQLQATAMSARAQESLPQPT---ISFAGQLHAALDRISDTQTAARTQAEKFTLGE 62

Query: 63 ADVSLSDVMIARNKSSVAFDATVQIRNKLVESYKDLMNMPV 103
V+L+DVM K+SV+ +Q+RNKLV +Y+++M+M V
Sbjct: 63 PGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03164HTHFIS489e-173 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 489 bits (1261), Expect = e-173
Identities = 173/482 (35%), Positives = 267/482 (55%), Gaps = 18/482 (3%)

Query: 1 MVQSKVLIVEDDEGLREALVDTLALAGYEWLEADCAEDALVKLKSNAVDIVVSDVQMAGM 60
M + +L+ +DD +R L L+ AGY+ A + + D+VV+DV M
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 GGLALLRNIRQNWPNMPVLLMTAYANIEDAVSAMKEGAIDYMAKPFAPEVLLNMVSR--- 117
LL I++ P++PVL+M+A A+ A ++GA DY+ KPF L+ ++ R
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 118 ------YAPIKSDDNGDAVVADEKSLR-LLALADKVARTDANVMILGPSGSGKEVMSRYI 170
+G +V +++ + + ++ +TD +MI G SG+GKE+++R +
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180

Query: 171 HNASNRKDGPFVAINCAAIPDNMLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEI 230
H+ R++GPFVAIN AAIP +++E+ LFG+EKGAFTGA G+FEQA+GGT+ LDEI
Sbjct: 181 HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI 240

Query: 231 SEMDLNLQAKLLRVLQEREVERLGSRKSIKLDVRVLATSNRDLKQYVSEGNFREDLYYRL 290
+M ++ Q +LLRVLQ+ E +G R I+ DVR++A +N+DLKQ +++G FREDLYYRL
Sbjct: 241 GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300

Query: 291 NVFPISWPALCERKGDISPLAGHLAERHCSKMGLPVPKFSQVALDKLLQYPWPGNVRELD 350
NV P+ P L +R DI L H ++ K GL V +F Q AL+ + +PWPGNVREL+
Sbjct: 301 NVVPLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALELMKAHPWPGNVRELE 359

Query: 351 NVVQRALILSENGDIDAEHILLEGVDWQDASSLQSVVQNAEVLVPDVK---PVAQVESIN 407
N+V+R L I E I E S ++ + L + Q +
Sbjct: 360 NLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASF 419

Query: 408 RVAAGGEGLGGELRDQ-EYAIILETLIECNGRRKEMAEKLGISPRTLRYKLAKMRDAGID 466
A GL + + EY +IL L G + + A+ LG++ TLR K+ ++ G+
Sbjct: 420 GDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIREL---GVS 476

Query: 467 IP 468
+
Sbjct: 477 VY 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03165PF06580320.003 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.1 bits (73), Expect = 0.003
Identities = 20/126 (15%), Positives = 47/126 (37%), Gaps = 24/126 (19%)

Query: 216 IDYFLEVEEEHTELLGNANAIASALSNLVMNAIQ--MSGKES--QIDVFFRPVNGELRIS 271
+ + ++ + + + LV N I+ ++ +I + NG + +
Sbjct: 240 LQFENQINPA----IMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLE 295

Query: 272 VQDSGPGVPKELQAKIMEPFFTTRSQGTGLGLAVVQMVCRA---HDGRLELISEQGDGAC 328
V+++G K + + TG GL V+ + + +++L +QG
Sbjct: 296 VENTGSLALKNTK------------ESTGTGLQNVRERLQMLYGTEAQIKLSEKQG-KVN 342

Query: 329 FTMCIP 334
+ IP
Sbjct: 343 AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03166HTHFIS5100.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 510 bits (1316), Expect = 0.0
Identities = 183/497 (36%), Positives = 268/497 (53%), Gaps = 31/497 (6%)

Query: 1 MQGLAKLLVIDDDAPSRLNISNILEFVGESCEAVSSDQLGDVDWSAVWSGFIVGNIS-AG 59
M G A +LV DDDA R ++ L G S+ +A +V ++
Sbjct: 1 MTG-ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPD 59

Query: 60 HAATAVMAHLNEAY-HIPLLVMGSFPLPVDDLPNFVGELEQ--------PLNYPQLSEAL 110
A ++ + +A +P+LVM + + E+ P + +L +
Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQN----TFMTAIKASEKGAYDYLPKPFDLTELIGII 115

Query: 111 SHCKDFLGRKGVNVVASARKNTLFRSLLGQSRGIQEVRHLIEQVSGTEANVLILGESGTG 170
R+ + ++ L+G+S +QE+ ++ ++ T+ ++I GESGTG
Sbjct: 116 GRALAEPKRRPSKLEDDSQD---GMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTG 172

Query: 171 KEVVARNIHYHSAYRNGPFVPINCGAIPPELLESELFGHEKGAFTGALTSRKGRFELAEG 230
KE+VAR +H + RNGPFV IN AIP +L+ESELFGHEKGAFTGA T GRFE AEG
Sbjct: 173 KELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEG 232

Query: 231 GTIFLDEIGDMPMSMQVKLLRVLQERCFERVGGNTTIKVNVRVVAATHRDLETMIDDGTF 290
GT+FLDEIGDMPM Q +LLRVLQ+ + VGG T I+ +VR+VAAT++DL+ I+ G F
Sbjct: 233 GTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLF 292

Query: 291 REDLFYRLNVFPIEMPALKARKQDIPLLLQELMTRLEAEGGQPICFTPRAINSLMEHNWP 350
REDL+YRLNV P+ +P L+ R +DIP L++ + + E EG F A+ + H WP
Sbjct: 293 REDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWP 352

Query: 351 GNVRELANLVERMIILYPNSLVDVNHLPTKYRYSDIPEFQPEGNPFISVEEQERDVFQDI 410
GNVREL NLV R+ LYP ++ + + R S+IP+ E S ++
Sbjct: 353 GNVRELENLVRRLTALYPQDVITREIIENELR-SEIPDSPIEKAAARSGSLSISQAVEEN 411

Query: 411 FSENFSFEDQSDLDHNMNAPQALPPEGVNLKELLADMEVNMISQALEAQGGVVARAADML 470
+ F+ + ALPP G+ +LA+ME +I AL A G +AAD+L
Sbjct: 412 MRQYFA-----------SFGDALPPSGL-YDRVLAEMEYPLILAALTATRGNQIKAADLL 459

Query: 471 GMRRTTLVEKMRKYNLQ 487
G+ R TL +K+R+ +
Sbjct: 460 GLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03169IGASERPTASE340.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.3 bits (78), Expect = 0.002
Identities = 52/319 (16%), Positives = 103/319 (32%), Gaps = 22/319 (6%)

Query: 233 NTDADQPEQVDADGNPIPPEEQKAAE------NAQDGATDEPISAAGAAAAKAGQEAIDA 286
N + ++ Q N P +A N + DE A A + A
Sbjct: 982 NPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVA 1041

Query: 287 ANKRAGMRPQDSIPGWTETASGTLLDSYRPPELELDEEAIAKAPDVPGWNNAASGTLTDS 346
N + + ++ + A+ T + E+ +EA + N A
Sbjct: 1042 ENSK---QESKTVEKNEQDATETTAQNR-----EVAKEAKSNVKANTQTNEVAQSGSETK 1093

Query: 347 YVTTKEAKQQLEQEKAEIEQKIADEKQELDQKVARGELTEEQAKQIHRAKLEPEERERLE 406
T E K+ EK E + ++ QE+ + + + KQ ++P+ E
Sbjct: 1094 ETQTTETKETATVEKEEKAKVETEKTQEVPKVTS-----QVSPKQEQSETVQPQAEPARE 1148

Query: 407 KIDVAEAKIAEAQKAFEVYSGMTEVQAGQDSQVMLDGVAQLSSHNNVIEDAIEGVNLTLK 466
K ++Q + + + + + +++ N+V+E+ T
Sbjct: 1149 NDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATT- 1207

Query: 467 GKSELGKPPAEIGVEYDRQSVRSDIENFVSAYNSFYQSSKALASVDPTTGQKGPLAGDST 526
+ + + R+SVRS N A S S +A D T+ + D+
Sbjct: 1208 -QPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS-TVALCDLTSTNTNAVLSDAR 1265

Query: 527 VRNADARLKSVFSTSIEKA 545
+ L + S +
Sbjct: 1266 AKAQFVALNVGKAVSQHIS 1284


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03171FLAGELLIN1799e-54 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 179 bits (454), Expect = 9e-54
Identities = 87/297 (29%), Positives = 136/297 (45%), Gaps = 3/297 (1%)

Query: 2 AINVNTNVSAMTAQRYLNQAAEGQQKSMERLSSGYKINSAKDDAAGLQISNRLNAQSRGL 61
A +NTN ++ Q LN++ ++ERLSSG +INSAKDDAAG I+NR + +GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DMAVKNANDGISIAQVAEGAMNESTNILQRMRDLSLQSANGSNSRSERVAIQEEVTALND 121
A +NANDGISIAQ EGA+NE N LQR+R+LS+Q+ NG+NS S+ +IQ+E+ +
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 ELNRIAETTSFGGNKLLNGTYGTQSFQIGADSGEAVMLSMGNLRSDTSAMGGKSYSAEDG 181
E++R++ T F G K+L+ Q+GA+ GE + + + + + + G + +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQD-NQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 KDASWAVGDNTELKMTYTNKQGEEKELT-INAKQGDDIEQLATYINGQSEDVKASVGEDG 240
+ T G K +N+ T + +
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 241 KLQVFAATQKVTGDVEFSGNLAGEIGFGDAKDVTVKD-IDVTTVAGSQEAVAIIDGA 296
+ A +G + G K D D V + + DG
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGN 296



Score = 122 bits (308), Expect = 5e-33
Identities = 75/269 (27%), Positives = 113/269 (42%), Gaps = 23/269 (8%)

Query: 126 IAETTSFGGNKLLNGTYGTQSFQIGADSGEAVMLSMGNLRSDTSAMGGKSYSAEDGKDAS 185
T + ++ I + + T + K+ + +GK ++
Sbjct: 242 ENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVST 301

Query: 186 WAVGDNTELKMTYTNKQGEEKELTINAKQGDDIEQL-ATYINGQSEDVKASVGEDGKLQV 244
G K+T T ++A + + + +NGQ + E KL
Sbjct: 302 TING----EKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSD 357

Query: 245 FAATQKVTGDVEFSGNLAGEIGFGDAKDVTV------------------KDIDVTTVAGS 286
A V G+ + + N A VT+ + +
Sbjct: 358 LEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKST 417

Query: 287 QEAVAIIDGALKSVDSQRASLGAFQNRFNHAISNLDNINENVNASNSRIKDTDYAKETTA 346
+A ID AL VD+ R+SLGA QNRF+ AI+NL N N+N++ SRI+D DYA E +
Sbjct: 418 ANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSN 477

Query: 347 MTKSQILQQASTSILAQAKQSPSAALSLL 375
M+K+QILQQA TS+LAQA Q P LSLL
Sbjct: 478 MSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03173FLAGELLIN1933e-59 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 193 bits (492), Expect = 3e-59
Identities = 87/297 (29%), Positives = 142/297 (47%), Gaps = 2/297 (0%)

Query: 2 AVNVNTNVSAMTAQRYLNNANSAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGL 61
A +NTN ++ Q LN + S+ +++ERLSSG +INSAKDDAAG I+NR +GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DVAVRNANDGISIAQTAEGAMNETTNILQRMRDLSLQSANGSNSKAERVAIQEEVTALND 121
A RNANDGISIAQT EGA+NE N LQR+R+LS+Q+ NG+NS ++ +IQ+E+ +
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 ELNRIAETTSFGGNKLLNGTYGTQSFQIGADNGEAVMLNLKDMRSDNQMMGGVSYQADNG 181
E++R++ T F G K+L+ Q+GA++GE + ++L+ + + + G +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQD-NQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 KDKNWNVEAGKNDLKISLTDSFGQEQEINISAKAGDDIEELATYINGQTDLVKASVDQDG 241
+ KN + +++++ A T +
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 242 KLQVFAGNNKVEGDVEFSGGLSGELGLNDGKKVTV-DTIDVTSVGGAQESVAIIDAA 297
+ + + +G + K DT D V ++ D
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGN 296



Score = 133 bits (336), Expect = 5e-37
Identities = 85/377 (22%), Positives = 141/377 (37%), Gaps = 21/377 (5%)

Query: 19 NNANSAQQTSMERLSSGFKINSAKDDAAGLQISNRLNVQSRGLDVAVRNANDGISIAQTA 78
N Q + ++ G L + ++ + +
Sbjct: 132 NGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKN 191

Query: 79 EGAMNETTNILQRMRDLSLQSANGSNSKAERVAIQEEVTALNDELNRIAETTSFGGNKLL 138
+ + R A +++ A V + V A N +L + +
Sbjct: 192 VTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFK 251

Query: 139 NGTYGTQSFQIGADNGEAVMLNLKDMRSDNQMMGGVSYQADNGKDKNWNVEAGKNDLKIS 198
+ + A G D + G D N V N K++
Sbjct: 252 TTKSTAGTAEAKAIAGAIKGGKEGDTFDYKG--VTFTIDTKTGNDGNGKVSTTINGEKVT 309

Query: 199 LTDSFGQEQEINISAKAGDDIEEL-ATYINGQTDLVKASVDQDGKLQVFAGNNKVEGDVE 257
LT + N+ A + + + +NGQ + ++ KL NN V+G+ +
Sbjct: 310 LTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESK 369

Query: 258 FSGGLSGELGLNDGKKVTVD------------------TIDVTSVGGAQESVAIIDAALK 299
+ + G KVT+ + +A ID+AL
Sbjct: 370 ITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDSALS 429

Query: 300 YVDSHRAELGAFQNRFNHAISNLDNINENVNASKSRIKDTDFAKETTQMTKSQILSQASS 359
VD+ R+ LGA QNRF+ AI+NL N N+N+++SRI+D D+A E + M+K+QIL QA +
Sbjct: 430 KVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGT 489

Query: 360 SILAQAKQAPNSALSLL 376
S+LAQA Q P + LSLL
Sbjct: 490 SVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03174FLAGELLIN2064e-64 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 206 bits (525), Expect = 4e-64
Identities = 92/297 (30%), Positives = 142/297 (47%), Gaps = 2/297 (0%)

Query: 2 AITVNTNVAALVAQRHLTSATDMLNQSMERLSSGKRINSAKDDAAGLQISNRLQSQMRGL 61
A +NTN +L+ Q +L + L+ ++ERLSSG RINSAKDDAAG I+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DVAVRNANDGISIMQTAEGAMNEVTNIMQRMRDLSLQSANGSNSKAERTALQEEVTALND 121
A RNANDGISI QT EGA+NE+ N +QR+R+LS+Q+ NG+NS ++ ++Q+E+ +
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 ELNRIAETTSFGGRKLLNGSFGKSAFQIGAASGEAVQVELKSMRSDGIDMGGFSYVANGR 181
E++R++ T F G K+L+ Q+GA GE + ++L+ + + + GF+
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQMK-IQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 ANSDWQVKDGANDLSMSFTNRFGETETIQINAKAGDDIEELTTYINGQTDKVTASVDEEG 241
A N + +N+ A T +
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 242 QLQLFMAGEETSGTLSFSGDL-ASELGLQLKGYDAVDNIDITSVGGAQQAVAVLDTA 297
+ A + T S +G A + +KG D D V D
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGN 296



Score = 127 bits (321), Expect = 6e-35
Identities = 59/213 (27%), Positives = 90/213 (42%), Gaps = 19/213 (8%)

Query: 183 NSDWQVKDGANDLSMSFTNRFGETETIQINAKAGDDIEEL-TTYINGQTDKVTASVDEEG 241
+ + +V N ++ T ++A + + T+ +NGQ + +E
Sbjct: 294 DGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESA 353

Query: 242 QLQLFMAGEETSGTLSFSGDLASELGLQLKGYDAVD------------------NIDITS 283
+L A G + + A + +
Sbjct: 354 KLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAA 413

Query: 284 VGGAQQAVAVLDTAMKYVDSHRAELGAYQNRFNHAINNLDNIHENLAASNSRIQDTDYAK 343
+A +D+A+ VD+ R+ LGA QNRF+ AI NL N NL ++ SRI+D DYA
Sbjct: 414 KKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYAT 473

Query: 344 ETTQMVKQQILQQVSTSILAQAKQAPNLALTLL 376
E + M K QILQQ TS+LAQA Q P L+LL
Sbjct: 474 EVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03177PF03309280.037 Bvg accessory factor
		>PF03309#Bvg accessory factor

Length = 271

Score = 28.2 bits (63), Expect = 0.037
Identities = 23/118 (19%), Positives = 41/118 (34%), Gaps = 25/118 (21%)

Query: 158 ALIKDGAFAGGS-----YVMVQRFEHNLPAWNRLNVSAQEKVIGRTKLDSIELEDVPAAS 212
+ G F GG+ V A R+ ++ VIG+ ++ ++ V
Sbjct: 140 VVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVELTRPRSVIGKNTVECMQAGAVFG-- 197

Query: 213 HVGRVD-----IKEE-----GKGLKIVRHSLPYGTATGAHGLLFISYCNTIHNIKVML 260
G VD I+++ G + +V ATG L + T+ + L
Sbjct: 198 FAGLVDGLVNRIRDDVDGFSGADVAVV--------ATGHTAPLVLPDLRTVEHYDRHL 247


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03179IGASERPTASE270.026 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 26.6 bits (58), Expect = 0.026
Identities = 18/84 (21%), Positives = 35/84 (41%), Gaps = 3/84 (3%)

Query: 12 SQETQTEAMKIAKSTQKPGQTKEQTKLIAQGIEKGIAQYKKQQKERNRQADKAKKKQQRE 71
++ETQT K + +K + K +T+ + + + K+ + + + + RE
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKV---TSQVSPKQEQSETVQPQAEPARE 1148

Query: 72 KQRQVDDVVEQESANIEAPTSKPA 95
V+ Q N A T +PA
Sbjct: 1149 NDPTVNIKEPQSQTNTTADTEQPA 1172



Score = 26.2 bits (57), Expect = 0.039
Identities = 15/88 (17%), Positives = 37/88 (42%), Gaps = 5/88 (5%)

Query: 11 PSQETQTEAMKIAKSTQKPGQTKEQTKLIAQGIEKGIAQ--YKKQQKERNRQADKAKKKQ 68
+ + T+ ++A+S + +T+ +EK ++ +E + + KQ
Sbjct: 1074 SNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQ 1133

Query: 69 QREKQRQVDDVVEQESA---NIEAPTSK 93
++ + Q +E+ NI+ P S+
Sbjct: 1134 EQSETVQPQAEPARENDPTVNIKEPQSQ 1161


55VIBHAR_03517VIBHAR_03523N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_03517-1140.297941hypothetical protein
VIBHAR_03518-2140.523017hypothetical protein
VIBHAR_03519-1121.102438protein-L-isoaspartate O-methyltransferase
VIBHAR_035200181.033492stationary phase survival protein SurE
VIBHAR_035212300.389956tRNA pseudouridine synthase D
VIBHAR_035222300.0635992-C-methyl-D-erythritol 2,4-cyclodiphosphate
VIBHAR_035232240.7855352-C-methyl-D-erythritol 4-phosphate
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03517OMADHESIN290.029 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

Length = 455

Score = 29.1 bits (64), Expect = 0.029
Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 3/79 (3%)

Query: 17 NASTEGIANNELEKTSNRTKTAAREE-FDASSKSLDATQLYLGEIGFSPLLTAEEEVLYA 75
++S GIANN + S T AR+E F S L+ + + + + L TAEE
Sbjct: 244 SSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSV 303

Query: 76 RRAL--RGDEAARKRMIES 92
R +E A K+ E+
Sbjct: 304 ARTTLETAEEHANKKSAEA 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03518RTXTOXIND353e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 35.2 bits (81), Expect = 3e-04
Identities = 16/65 (24%), Positives = 33/65 (50%), Gaps = 12/65 (18%)

Query: 222 IVSTAAGTVVYSGNALRGYGNLIIVKHNDNYLSAYAHNDRLLVSEGQSVKSGQKIATMGS 281
IV+TA G + +SG + +K +N + ++V EG+SV+ G + + +
Sbjct: 82 IVATANGKLTHSGRSK-------EIKPIEN-----SIVKEIIVKEGESVRKGDVLLKLTA 129

Query: 282 SGSKS 286
G+++
Sbjct: 130 LGAEA 134


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03519PF05272280.022 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.022
Identities = 7/36 (19%), Positives = 16/36 (44%), Gaps = 2/36 (5%)

Query: 89 QTAVLAQIVDHVYS--VERIKSLQWDAKRRLKQLDI 122
+ A+ + + +K+ QWD RL++ +
Sbjct: 520 EQAINVAADMNRVHPFRDWVKAQQWDEVPRLEKWLV 555


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03523MPTASEINHBTR290.007 Metalloprotease inhibitor signature.
		>MPTASEINHBTR#Metalloprotease inhibitor signature.

Length = 122

Score = 29.2 bits (65), Expect = 0.007
Identities = 15/61 (24%), Positives = 26/61 (42%), Gaps = 3/61 (4%)

Query: 181 QQLTQALADALEQGATIT-DEASALEWLGEKPALVQGNANNIKITQPEDLALAEFYLSRE 239
+ A EQ + D A A +WLG+KP + I + E + +L+R+
Sbjct: 44 ATGSGVCAGPAEQANALAGDVACAEQWLGDKPVSWSPTPDGIWLMNAEGTGIT--HLNRQ 101

Query: 240 R 240
+
Sbjct: 102 K 102


56VIBHAR_03685VIBHAR_03698N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
VIBHAR_03685018-1.997408rod shape-determining protein MreB
VIBHAR_03686021-2.601921hypothetical protein
VIBHAR_03687022-1.362952hypothetical protein
VIBHAR_03688-220-1.264008hypothetical protein
VIBHAR_03689021-0.847539hypothetical protein
VIBHAR_03690-118-0.135375hypothetical protein
VIBHAR_036910160.242783hypothetical protein
VIBHAR_036920160.477746hypothetical protein
VIBHAR_03693-2140.551612hypothetical protein
VIBHAR_03694-2140.697033hypothetical protein
VIBHAR_03695-2140.399419hypothetical protein
VIBHAR_03696-114-0.054820hypothetical protein
VIBHAR_03697-214-0.676172Type II secretory pathway, component ExeA
VIBHAR_03698-212-0.906517hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03685SHAPEPROTEIN5670.0 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 567 bits (1463), Expect = 0.0
Identities = 318/347 (91%), Positives = 334/347 (96%)

Query: 1 MFKKLRGMFSNDLSIDLGTANTLIYVKGQGIVLDEPSVVAIRQDRVGSAKSVAAVGHAAK 60
M KK RGMFSNDLSIDLGTANTLIYVKGQGIVL+EPSVVAIRQDR GS KSVAAVGH AK
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAAVGHDAK 60

Query: 61 QMLGRTPGNISAIRPMKDGVIADFYVTEKMLQHFIKQVHDNSILKPSPRVLVCVPCGSTQ 120
QMLGRTPGNI+AIRPMKDGVIADF+VTEKMLQHFIKQVH NS ++PSPRVLVCVP G+TQ
Sbjct: 61 QMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQ 120

Query: 121 VERRAIRESALGAGAREVYLIDEPMAAAIGAGLRVSEPTGSMVVDIGGGTTEVAVISLNG 180
VERRAIRESA GAGAREV+LI+EPMAAAIGAGL VSE TGSMVVDIGGGTTEVAVISLNG
Sbjct: 121 VERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNG 180

Query: 181 VVYSSSVRIGGDRFDEAVINYVRRNYGSLIGEATAEKIKHEIGSAYPGDEVQEIEVRGRN 240
VVYSSSVRIGGDRFDEA+INYVRRNYGSLIGEATAE+IKHEIGSAYPGDEV+EIEVRGRN
Sbjct: 181 VVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGRN 240

Query: 241 LAEGVPRSFSLNSNEILEALQEPLSGIVSAVMVALEQCPPELASDISENGMVLTGGGALL 300
LAEGVPR F+LNSNEILEALQEPL+GIVSAVMVALEQCPPELASDISE GMVLTGGGALL
Sbjct: 241 LAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGALL 300

Query: 301 KDLDRLLMEETGIPVVIAEDPLTCVARGGGKALEMIDMHGGDLFSEE 347
++LDRLLMEETGIPVV+AEDPLTCVARGGGKALEMIDMHGGDLFSEE
Sbjct: 301 RNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSEE 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03687BCTERIALGSPG280.006 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 28.3 bits (63), Expect = 0.006
Identities = 9/27 (33%), Positives = 18/27 (66%)

Query: 1 MFRKNAQQGNVLIVVLFVIIVMGYLAA 27
M + Q+G L+ ++ VI+++G LA+
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVLAS 27


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03688BCTERIALGSPG328e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.2 bits (73), Expect = 8e-04
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 6/50 (12%)

Query: 2 KTRGFTLMEMIVTIVIGSFIMLGI-AGYVQLGMKGYADTIDRQRMQTQAQ 50
K RGFTL+E++V IVI +G+ A V + G + D+Q+ +
Sbjct: 6 KQRGFTLLEIMVVIVI-----IGVLASLVVPNLMGNKEKADKQKAVSDIV 50


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03689BCTERIALGSPG280.016 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 27.9 bits (62), Expect = 0.016
Identities = 12/34 (35%), Positives = 20/34 (58%), Gaps = 4/34 (11%)

Query: 3 MRKQLGMTLAEMIIAIVLMGIAMVAFTSFLVPQI 36
KQ G TL E+++ IV++G+ S +VP +
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGV----LASLVVPNL 33


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03691BCTERIALGSPG356e-06 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 35.2 bits (81), Expect = 6e-06
Identities = 11/32 (34%), Positives = 20/32 (62%)

Query: 1 MRKSSGLSAIEFVVVLVILGVVGYVVLPRFIS 32
K G + +E +VV+VI+GV+ +V+P +
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMG 35


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03692BCTERIALGSPG536e-12 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 53.4 bits (128), Expect = 6e-12
Identities = 19/54 (35%), Positives = 31/54 (57%), Gaps = 4/54 (7%)

Query: 1 MKRQGGFTLIELVVVIVILGILAVTAAPRFLNLQNDARA----SSLQGLKGAIE 50
+Q GFTL+E++VVIVI+G+LA P + + A S + L+ A++
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALD 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03694BCTERIALGSPF2802e-93 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 280 bits (719), Expect = 2e-93
Identities = 114/399 (28%), Positives = 197/399 (49%), Gaps = 2/399 (0%)

Query: 1 MPTFRYQGRTLDGSSTSGKVDAVNSEAAAEALMNKGIIPLNLRLEKEAVKSKVSLSKLLV 60
M + YQ G G +A ++ A + L +G++PL++ + + S L
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 61 PAIPLEV--IILFSRQLFSLTKAGVPLLRSMRGLLQNCENKQLKEALEEVVAELSNGRGL 118
I L + L +RQL +L A +PL ++ + + E L + + V +++ G L
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 119 SSAMQPHTKVFSPLFVSMINVGENTGRLDQALLQLANYYEQELETRKRIKAAMRYPTFVI 178
+ AM+ F L+ +M+ GE +G LD L +LA+Y EQ + R RI+ AM YP +
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 179 VFITIAMFILNILVIPEFASMFTRFGVELPLPTRILIATSSFFVNYWGLLIAAIIGAFVI 238
V + IL +V+P+ F LPL TR+L+ S + ++ A++ F+
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 239 FRSWVATADGREKFDKLRLRLPIVGDIVNRAQLSRFARTFSLMLKSGVPLNQSLALAGEA 298
FR + R F + L LP++G I +R+ART S++ S VPL Q++ ++G+
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 299 LGNKFLENRILEMKAAIEAGSTISVTAINSKIFTPLVIQMIAVGEETGRIDELLLEVSDF 358
+ N + +R+ A+ G ++ + +F P++ MIA GE +G +D +L +D
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 359 YDREVDYDLKTLTARIEPLLLVIVAGMVMVLALGIFLPM 397
DRE + EPLL+V +A +V+ + L I P+
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPI 399



Score = 69.5 bits (170), Expect = 4e-15
Identities = 32/126 (25%), Positives = 58/126 (46%), Gaps = 1/126 (0%)

Query: 71 FSRQLFSLTKAGVPLLRSMRGLLQNCENKQLKEALEEVVAELSNGRGLSSAMQPHTKVFS 130
++R L L + VPLL++MR N + L + G L A++ T +F
Sbjct: 276 YARTLSILNASAVPLLQAMRISGDVMSNDYARHRLSLATDAVREGVSLHKALE-QTALFP 334

Query: 131 PLFVSMINVGENTGRLDQALLQLANYYEQELETRKRIKAAMRYPTFVIVFITIAMFILNI 190
P+ MI GE +G LD L + A+ ++E ++ + + P V+ + +FI+
Sbjct: 335 PMMRHMIASGERSGELDSMLERAADNQDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLA 394

Query: 191 LVIPEF 196
++ P
Sbjct: 395 ILQPIL 400


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
VIBHAR_03698BCTERIALGSPD1751e-49 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 175 bits (445), Expect = 1e-49
Identities = 76/314 (24%), Positives = 138/314 (43%), Gaps = 27/314 (8%)

Query: 220 QQAVANLIGSGKGQSVVVTPQAGVITVRAFPDEIREVREFLGVSQERMQRQVILEAKILE 279
+QA + K + Q + V A PD + ++ + + + QV++EA I E
Sbjct: 297 KQAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLERVIA-QLDIRRPQVLVEAIIAE 355

Query: 280 VTLSDGYQQGINWSNMSASI---GNSGSILVDRNPAKLPLDAIGTLLGGQTN-------- 328
V +DG GI W+N +A + NSG + + GT+ +
Sbjct: 356 VQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGI 415

Query: 329 -VTFSDGNFEAVMSFMATQGDLNVLSSPRITAANNQKSVIKVGTDEYFVTELSSNAGNGE 387
F GN+ +++ +++ ++L++P I +N ++ VG + +T S +G+
Sbjct: 416 AAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLT--GSQTTSGD 473

Query: 388 NSNAVPEVELTPFFSGISLDVTPQIDNKGNVFLHVHPAVIEVEEEIKQLNLGGDFQNIQL 447
N E + GI L V PQI+ +V L + V V + +
Sbjct: 474 NIFNTVERKTV----GIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLG------ 523

Query: 448 PLAKSSIRESDSVIRAKDGDVVVIGGLMKQQNLEQVSKVPFLGDVPALGNLFRNINNVTQ 507
A + R ++ + G+ VV+GGL+ + + KVP LGD+P +G LFR+ +
Sbjct: 524 --ATFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVS 581

Query: 508 KTELVILLKPTVVG 521
K L++ ++PTV+
Sbjct: 582 KRNLMLFIRPTVIR 595



Score = 38.0 bits (88), Expect = 8e-05
Identities = 36/196 (18%), Positives = 67/196 (34%), Gaps = 48/196 (24%)

Query: 71 FRVQANAVEARSFFASLVKGTEYSVAIHPAVQGNITVDLSDVT----LDEVLNVVQNMYG 126
F + + F ++ K +V I P+V+G ITV D+ + V ++YG
Sbjct: 30 FSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVLDVYG 89

Query: 127 YDVVKSGK-VIQVYPA-GMRTVTIPVDYLQFKRSGRSLTSIVTGSVTSAGTSNSGGGSDD 184
+ V+ V++V + +T +PV
Sbjct: 90 FAVINMNNGVLKVVRSKDAKTAAVPV---------------------------------- 115

Query: 185 SDSSDSNNNNGDGSTTSTGGTRIETITESDFWPMLQQAVANLIGSGKGQSVVVTPQAGVI 244
+SD+ GD T + + D P+L+Q L + SVV + V+
Sbjct: 116 --ASDAAPGIGDEVVTRV--VPLTNVAARDLAPLLRQ----LNDNAGVGSVVHYEPSNVL 167

Query: 245 TVRAFPDEIREVREFL 260
+ I+ + +
Sbjct: 168 LMTGRAAVIKRLLTIV 183



 
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