PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
GenomeUM032.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in CP005490 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1K747_08870K747_09085Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_08870312-1.013573hypothetical protein
K747_08875312-1.181234hypothetical protein
K747_08880313-0.894316hypothetical protein
K747_08885214-0.990204hypothetical protein
K747_08890219-1.657566hypothetical protein
K747_08895017-0.403336hypothetical protein
K747_089050140.336958hypothetical protein
K747_089150151.419401cell division protein
K747_089200131.589049hypothetical protein
K747_089250131.688268hypothetical protein
K747_089301112.371014hypothetical protein
K747_089350122.130339urease accessory protein UreH
K747_089404233.007908urease accessory protein UreG
K747_089454232.608509urease accessory protein UreF
K747_089504222.170509urease accessory protein UreE
K747_089552192.042870acid-activated urea channel
K747_089601181.526470hypothetical protein
K747_089651162.218679urease subunit alpha
K747_08970-2101.222566urease subunit beta
K747_08980-1101.008093*peptidase A8
K747_089850142.105617phosphoglucosamine mutase
K747_089902162.65375230S ribosomal protein S20
K747_089954142.803668peptide chain release factor 1
K747_090006182.587754outer membrane protein HorA
K747_090056172.473084outer membrane protein HorA
K747_090105172.446231hypothetical protein
K747_090151151.738417hypothetical protein
K747_090200131.238552hypothetical protein
K747_090300140.12884230S ribosomal protein S9
K747_09035014-0.31909950S ribosomal protein L13
K747_09040-111-1.756481hypothetical protein
K747_09045-111-1.975412malate:quinone oxidoreductase
K747_09050013-2.828270hypothetical protein
K747_09055416-5.321703RNA polymerase sigma factor RpoD
K747_09060623-7.067929hypothetical protein
K747_09065523-6.918817hypothetical protein
K747_09070525-5.850524virB2 type IV secretion protein
K747_09075524-5.241597virB3 type IV secretion protein
K747_09080522-4.947546hypothetical protein
K747_09085220-2.263497DNA topoisomerase I
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_08880GPOSANCHOR330.001 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 32.7 bits (74), Expect = 0.001
Identities = 32/195 (16%), Positives = 67/195 (34%), Gaps = 3/195 (1%)

Query: 15 REELEARISELEDENENLTDENTRLLASKEWPTKENAELLREKDNLTKANAELKKENDKL 74
+ +LE + + + + + L A K AEL + + + + L
Sbjct: 157 KADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTL 216

Query: 75 NHQIITLTKEQDSLKYERVQLKDAHGSLKELCANSEKDNQRLADKLKKLESAQKNLANSN 134
+ L + L+ + + E + L + +LE A + N +
Sbjct: 217 EAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFS 276

Query: 135 NQLLQAREKIAKEKTELERKMARLKSLEATDKSELDLQNRRF---KSAIEDLKRQNRKLE 191
+ + EK LE + A L+ + R + A + L+ +++KLE
Sbjct: 277 TADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLE 336

Query: 192 EENIALKERVDGLKE 206
E+N + L+
Sbjct: 337 EQNKISEASRQSLRR 351



Score = 32.3 bits (73), Expect = 0.001
Identities = 36/192 (18%), Positives = 70/192 (36%), Gaps = 4/192 (2%)

Query: 16 EELEARISELEDENENLTDENTRLLASKEWPTKENAELLREKDNLTKANAELKKENDKLN 75
+ L+ EL +E N ++ + S + EL K +L KA + +
Sbjct: 81 KALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADS 140

Query: 76 HQIITLTKEQDSLKYERVQLKDAHGSLKELCANSEKDNQRLADKLKKLESAQKNLANSNN 135
+I TL E+ +L + L+ A + L + LE+ Q L
Sbjct: 141 AKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAEL----E 196

Query: 136 QLLQAREKIAKEKTELERKMARLKSLEATDKSELDLQNRRFKSAIEDLKRQNRKLEEENI 195
+ L+ + + + + K+ A K++L+ + + + LE E
Sbjct: 197 KALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKA 256

Query: 196 ALKERVDGLKEQ 207
AL+ R L++
Sbjct: 257 ALEARQAELEKA 268



Score = 30.8 bits (69), Expect = 0.003
Identities = 30/198 (15%), Positives = 60/198 (30%), Gaps = 10/198 (5%)

Query: 13 QVREELEARISELEDENENLTDENTRLLASKEWPTKENAELLREKDNLTKANAELKKEND 72
+ E + + + L E L A A+L + + + +
Sbjct: 127 KALEGAMNFSTADSAKIKTLEAEKAALAA-------RKADLEKALEGAMNFSTADSAKIK 179

Query: 73 KLNHQIITLTKEQDSLKYERVQLKDAHGSLKELCANSEKDNQRLADKLKKLESAQKNLAN 132
L + L Q L+ + + E + LA + LE A + N
Sbjct: 180 TLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMN 239

Query: 133 SNNQLLQAREKIAKEKTELERKMARLKSLEATDKSELDLQNRRFKSA---IEDLKRQNRK 189
+ + + EK LE + A L+ + + + K+ L+ +
Sbjct: 240 FSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKAD 299

Query: 190 LEEENIALKERVDGLKEQ 207
LE ++ L L+
Sbjct: 300 LEHQSQVLNANRQSLRRD 317


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_08965UREASE10450.0 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 1045 bits (2704), Expect = 0.0
Identities = 354/569 (62%), Positives = 443/569 (77%), Gaps = 4/569 (0%)

Query: 3 KISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSN-NP 61
++SR Y +M+GPT GDKVRL DT+L EVE D+T +GEE+KFGGGK +R+GM QS
Sbjct: 4 RMSRAAYANMFGPTVGDKVRLADTELFIEVEKDFTTHGEEVKFGGGKVIRDGMGQSQVTR 63

Query: 62 SKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEAL 121
+D +ITNALI+D+ GI KADIG+KDG+IA IGK GN DMQ GV + VGP TE +
Sbjct: 64 EGGAVDTVITNALILDHWGIVKADIGLKDGRIAAIGKAGNPDMQPGV--TIIVGPGTEVI 121

Query: 122 AGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKW 181
AGEG IVTAGG+D+HIHFI PQQI A SG+T M+GGGTGPA GT ATT TPG ++
Sbjct: 122 AGEGKIVTAGGMDSHIHFICPQQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIAR 181

Query: 182 MLRAAEEYSMNLGFLAKGNTSNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKY 241
M+ AA+ + MNL F KGN S +L + + GA K+HEDWGTTP+AI+ L VAD+Y
Sbjct: 182 MIEAADAFPMNLAFAGKGNASLPGALVEMVLGGATSLKLHEDWGTTPAAIDCCLSVADEY 241

Query: 242 DVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTN 301
DVQV IHTDTLNE+G VEDT+AAI GRT+H +HTEGAGGGHAPDII++ G+ N++P+STN
Sbjct: 242 DVQVMIHTDTLNESGFVEDTIAAIKGRTIHAYHTEGAGGGHAPDIIRICGQPNVIPSSTN 301

Query: 302 PTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSS 361
PT P+TVNT AEH+DMLMVCHHL +I ED+ FA+SRIR +TIAAED LHD+G FSI SS
Sbjct: 302 PTRPYTVNTLAEHLDMLMVCHHLSPTIPEDIAFAESRIRKETIAAEDILHDIGAFSIISS 361

Query: 362 DSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISE 421
DSQAMGRVGEV RTWQTADK K++ GRLKEE GDNDNFR+KRY++KYTINPAIAHG+S
Sbjct: 362 DSQAMGRVGEVAIRTWQTADKMKRQRGRLKEETGDNDNFRVKRYIAKYTINPAIAHGLSH 421

Query: 422 YVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAH 481
+GS+EVGK ADLVLW+PAFFGVKP+M++ GG IA + MGD NASIPTPQPV+YR MF
Sbjct: 422 EIGSLEVGKRADLVLWNPAFFGVKPDMVLLGGTIAAAPMGDPNASIPTPQPVHYRPMFGA 481

Query: 482 HGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCR-NITKKDMQFNDTTAHIEVNP 540
+G+++ ++++TFVSQA+ D G+ LG+ ++++ V+N R I K M N T HIEV+P
Sbjct: 482 YGRSRTNSSVTFVSQASLDAGLAGRLGVAKELVAVQNTRGGIGKASMIHNSLTPHIEVDP 541

Query: 541 ETYHVFVDGKEVTSKPATKVSLAQLFSIF 569
ETY V DG+ +T +PAT + +AQ + +F
Sbjct: 542 ETYEVRADGELLTCEPATVLPMAQRYFLF 570


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09015OUTRMMBRANEA280.025 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 28.0 bits (62), Expect = 0.025
Identities = 23/89 (25%), Positives = 36/89 (40%), Gaps = 13/89 (14%)

Query: 47 TKRFGFRTYGYYSYNHANLSFVG-----------SKLGIMDGASQVNNFTYGVGFDALYN 95
TK F ++ +++N A L G S L DG+ V +T +G DA YN
Sbjct: 212 TKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDA-YN 270

Query: 96 FYESKERYNT-AGLFLGFGLGGDSFIVQG 123
S+ R + + G+ D +G
Sbjct: 271 QGLSERRAQSVVDYLISKGIPADKISARG 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09020TACYTOLYSIN310.011 Bacterial thiol-activated pore-forming cytolysin sig...
		>TACYTOLYSIN#Bacterial thiol-activated pore-forming cytolysin

signature.
Length = 574

Score = 31.5 bits (71), Expect = 0.011
Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 2/55 (3%)

Query: 503 MNNTDTGNTDTGNTDTGNTDTGNTDTGNADDMSNMNNGNDDMGNTNDDMSNSNDM 557
+ N T N D+ +T NT+T T+ + S + + G DDM NSNDM
Sbjct: 26 VGNLVTANADSNKQNTANTETTTTNEQPKPESSELT--TEKAGQKMDDMLNSNDM 78


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09055IGASERPTASE340.003 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 33.9 bits (77), Expect = 0.003
Identities = 31/186 (16%), Positives = 59/186 (31%), Gaps = 35/186 (18%)

Query: 8 EKAPKRAKQEAKTEATQENKIKE-----------------------------SKIKEAKT 38
E + +KQE+KT E E S+ KE +T
Sbjct: 1038 ETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQT 1097

Query: 39 KESKIKETKTKESKIK---ETKAKEPVPVKKLSFNEALEELF---ANSLSDCVSYESIIQ 92
E+K T KE K K E + P ++S + E A + +I +
Sbjct: 1098 TETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKE 1157

Query: 93 ISAKVPTLAQVKKIKELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEKQKVLDEELEDGYD 152
++ T A ++ + ++ V+ S N++ + + + +
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSN 1217

Query: 153 FLKEKD 158
K +
Sbjct: 1218 KPKNRH 1223


2K747_09260K747_09290Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_09260018-3.390919S-adenosylhomocysteine nucleosidase
K747_09265322-4.259367hypothetical protein
K747_09270323-3.330341integrase
K747_09275323-3.262169CAAX protease
K747_092802181.385345hypothetical protein
K747_092851170.826926hypothetical protein
K747_092902170.617514hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09290HELNAPAPROT320.001 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 31.8 bits (72), Expect = 0.001
Identities = 23/103 (22%), Positives = 47/103 (45%), Gaps = 8/103 (7%)

Query: 133 FNWLYKGSDFGALHEQFGDMYDGYIKYLISMVRISQKEKARKLDAVTLKKMEDQAEKD-- 190
F+W KG F LHE+F ++YD + + ++ E+ + + +++ E
Sbjct: 34 FHWYVKGPHFFTLHEKFEELYDHAAETVDTIA-----ERLLAIGGQPVATVKEYTEHASI 88

Query: 191 TKAAFQKRSSGELESLTDSPEFISSS-KKVQNTSNSDLDSTTN 232
T + +S +++L + + ISS K V + + D+ T
Sbjct: 89 TDGGNETSASEMVQALVNDYKQISSESKFVIGLAEENQDNATA 131


3K747_09355K747_09400Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
K747_093552122.246710lactate permease
K747_093603111.5632482-hydroxyacid dehydrogenase
K747_093654122.315297hypothetical protein
K747_093704122.152398ABC transporter permease
K747_093753120.816953cytochrome oxidase subunit I
K747_09380316-1.413257peptidase S13
K747_09385619-3.069387cytochrome B561
K747_09395315-0.186820hypothetical protein
K747_09400213-0.020861hypothetical protein
4K747_09585K747_09675Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_09585-1113.296463hypothetical protein
K747_09590-1102.888283hypothetical protein
K747_09595-192.183517hypothetical protein
K747_09600-292.312060hypothetical protein
K747_09605-1103.054030fumarate reductase
K747_09610-2103.182397fumarate reductase
K747_09615-2141.768228fumarate reductase
K747_09620-3151.799497triosephosphate isomerase
K747_09625-2172.799777enoyl-ACP reductase
K747_09630-2162.967400UDP-3-O-(3-hydroxymyristoyl) glucosamine
K747_09635-2143.245072S-adenosylmethionine synthetase
K747_09640-2162.349973nucleoside diphosphate kinase
K747_09645-2181.774338hypothetical protein
K747_09650-2141.48224550S ribosomal protein L32
K747_09655-1120.940901phosphate acyltransferase
K747_09660213-3.6343583-oxoacyl-ACP synthase
K747_09665313-3.131604hypothetical protein
K747_09670212-3.238075hypothetical protein
K747_09675112-3.181321hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09625DHBDHDRGNASE615e-13 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 60.8 bits (147), Expect = 5e-13
Identities = 61/263 (23%), Positives = 109/263 (41%), Gaps = 29/263 (11%)

Query: 4 LKGKKGLIVGVANNKSIAYGIAQSCFNQGATL-AFTYLNESLEKRVRPIAQELNSPYVYE 62
++GK I G A + I +A++ +QGA + A Y E LEK V + E +
Sbjct: 6 IEGKIAFITGAA--QGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 63 LDVSKEEHFKPLYDSVKKDLGSLDFIVHSVAF--------APKEALEGSLLETSKSAFNT 114
DV + +++++G +D +V+ E E + S FN
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 115 AMEISVYSLIELTNTLKPLLNNGASVLTLSYLGSTKYMAHYNVMGLAKAALESAVRYLAV 174
+ +S Y + + ++ + +N A V S MA Y +KAA + L +
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTS-------MAAY---ASSKAAAVMFTKCLGL 173

Query: 175 DLGKHNIRVNALSAGPIRT-----LASSGIADFRMILKWNE---INAPLRKNVSLEEVGN 226
+L ++NIR N +S G T L + ++I E PL+K ++ +
Sbjct: 174 ELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIAD 233

Query: 227 AGMYLLSNLSSGVSGEVHFVDAG 249
A ++L+S + ++ VD G
Sbjct: 234 AVLFLVSGQAGHITMHNLCVDGG 256


5K747_10155K747_10375Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_101551153.28240150S ribosomal protein L21
K747_101601153.25977250S ribosomal protein L27
K747_101650143.283782peptide ABC transporter substrate-binding
K747_101700143.630580peptide ABC transporter permease
K747_10175-1142.949843peptide ABC transporter
K747_10180-3132.674198peptide ABC transporter ATP-binding protein
K747_10185-2132.508795ABC transporter ATP-binding protein
K747_10190-2132.196921GTPase CgtA
K747_10195-1162.372262alginate lyase
K747_102000182.337538polyisoprenoid-binding protein
K747_102051172.462901glutamate-1-semialdehyde aminotransferase
K747_102104151.440901arginine biosynthesis bifunctional protein ArgJ
K747_102153121.591202membrane protein
K747_102250120.930287hypothetical protein
K747_102300130.114597hypothetical protein
K747_10235013-0.288324ATP-binding protein
K747_10240116-0.931364MFS transporter
K747_10255217-1.553251hypothetical protein
K747_10260315-1.947988arginyl-tRNA synthetase
K747_10265317-1.761022preprotein translocase subunit TatA
K747_10270215-1.373071guanylate kinase
K747_10275114-1.466208hypothetical protein
K747_10280-211-1.546955hypothetical protein
K747_10285-212-2.036511nuclease
K747_10290011-2.151172membrane protein
K747_10295213-2.256630flagellar L-ring protein FlgH
K747_10300313-1.794821CMP-N-acetylneuraminic acid synthetase
K747_10305211-1.011374CMP-N-acetylneuraminic acid synthetase
K747_10310212-0.787834flagellar protein FlaG
K747_103152120.951398tetraacyldisaccharide 4'-kinase
K747_103201152.013608NAD synthetase
K747_103300172.796236*ketol-acid reductoisomerase
K747_103350182.267872septum site-determining protein MinD
K747_103400171.734808cell division topological specificity factor
K747_103452191.671605DNA processing protein DprA
K747_103505221.827374Holliday junction resolvase
K747_103557230.632984hypothetical protein
K747_10360623-0.089880hypothetical protein
K747_103655230.535070hypothetical protein
K747_10370418-0.838875hypothetical protein
K747_10375217-0.185291hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10240TCRTETA479e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 46.7 bits (111), Expect = 9e-08
Identities = 57/271 (21%), Positives = 105/271 (38%), Gaps = 16/271 (5%)

Query: 28 LILSGSLTPHQSFQLGIAVLMGYVFGSFLIQFLSPLMSLESIAKISFGLIALSFLICYFD 87
L+ S +T H L + LM + L LS + +S A+ + I
Sbjct: 35 LVHSNDVTAHYGILLALYALMQFACAPVLGA-LSDRFGRRPVLLVSLAGAAVDYAI--MA 91

Query: 88 SIPFFW-LWIWRFIAGVASSALMILVAPLSLPYVKENKKALVGGFIFSAVGIGSVFSGFV 146
+ PF W L+I R +AG+ + A + +++A GF+ + G G V +
Sbjct: 92 TAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVL 150

Query: 147 LPWISSYNIKWAWIFLGGSCLIAFILSLIGLKN-HSLKKKSVKKEESAFKIPFHL----- 200
+ ++ + + F+ L H +++ +++E F
Sbjct: 151 GGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMT 210

Query: 201 ---WLLLVSCALNAIGFLPHTLFWVDYLIRHLNISPTTAGTSWALFG-FGATLGSLISGP 256
L+ V + +G +P L WV + + TT G S A FG + ++I+GP
Sbjct: 211 VVAALMAVFFIMQLVGQVPAAL-WVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGP 269

Query: 257 MAQKLGAKNANIFILILKSIACFLPIFFHQI 287
+A +LG + A + +I L F +
Sbjct: 270 VAARLGERRALMLGMIADGTGYILLAFATRG 300


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10270PF05272290.011 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.011
Identities = 9/18 (50%), Positives = 11/18 (61%)

Query: 8 LILSGPSGAGKSTLTKYL 25
++L G G GKSTL L
Sbjct: 599 VVLEGTGGIGKSTLINTL 616


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10275IGASERPTASE701e-14 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 69.7 bits (170), Expect = 1e-14
Identities = 46/279 (16%), Positives = 85/279 (30%), Gaps = 4/279 (1%)

Query: 140 ELENLGDLEALVQEEPNNEEQLLPTLNAQEEKEEVKEETKETPQEEEEKPKDDEVQEGET 199
E+E N Q +E + + P P + E
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAEN 1043

Query: 200 QKNEEVSKELETQEELEIPKEETQEQVKEQEPIKEETQENKEEKQEKTQDSPSTQELEAM 259
K E + E Q+ E + + + + +K TQ N+ + T E +
Sbjct: 1044 SKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKET 1103

Query: 260 QELVKEIQENSNGQEDKKETQESAETPQEKETQELETPQNVEIPQESAKTPQETEKQELE 319
+ KE + ++ ++ + +++ ++E E PQ + + + +
Sbjct: 1104 ATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTN 1163

Query: 320 TPQEETQESAETPQETEKQELEIPQNVETPQEKETPQETPQETSQETSQESAEKTQKLET 379
T + Q + ET E+ E E P+ T T+Q T + K
Sbjct: 1164 TTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPK--- 1220

Query: 380 QEDHYESIADIPEPVMAQAMGEELPFLNESVAKTSNNEN 418
H S+ +P V TS N N
Sbjct: 1221 -NRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTN 1258



Score = 64.3 bits (156), Expect = 7e-13
Identities = 44/240 (18%), Positives = 84/240 (35%), Gaps = 9/240 (3%)

Query: 215 LEIPKEETQEQVKEQEPIKEETQENKEEKQEKTQDSPSTQELEAMQELVKEIQENSNGQE 274
++ T ++ P E E P+ E V E + +
Sbjct: 992 VDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTV 1051

Query: 275 DKKETQESAETPQEKETQELETPQNVEIPQESAKTPQETEKQELETPQEETQESAETPQE 334
+K E + T Q +E + E Q E + + +ETQ + +E
Sbjct: 1052 EKNEQDATETTAQNREVAK-------EAKSNVKANTQTNEVAQSGSETKETQTTET--KE 1102

Query: 335 TEKQELEIPQNVETPQEKETPQETPQETSQETSQESAEKTQKLETQEDHYESIADIPEPV 394
T E E VET + +E P+ T Q + ++ E+ + + + D +I +
Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQT 1162

Query: 395 MAQAMGEELPFLNESVAKTSNNENDTETPKESVIKTPQEKEESIETPKESDKTSSPLELR 454
A E+ S + E+ T SV++ P+ + P + ++S+ + R
Sbjct: 1163 NTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNR 1222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10295FLGLRINGFLGH1905e-63 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 190 bits (485), Expect = 5e-63
Identities = 51/172 (29%), Positives = 84/172 (48%), Gaps = 18/172 (10%)

Query: 56 GERPLFADRRAMKPNDLITIIVSEKASANYSSS----KDYKSTSGGNSTPPRLTYNGLDE 111
G +PLF DRR D +TI++ E SA+ SSS +D K+ G ++ P L GL
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYL--QGLFG 118

Query: 112 RKKQEAQYLDDKNNYNFTKSSNNTNFKGGGSQKKSEDLEIVLSARIIKVLENGNYFIYGN 171
+ + + S F G G S L+ + +VL NGN + G
Sbjct: 119 NARADVEA------------SGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGE 166

Query: 172 KEVLVDGEKQILKVSGVIRPYDIERNNTIQSKFLADAKIEYTNLGHLSDSNK 223
K++ ++ + ++ SGV+ P I +NT+ S +ADA+IEY G+++++
Sbjct: 167 KQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN 218


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10310SACTRNSFRASE280.015 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.0 bits (62), Expect = 0.015
Identities = 18/67 (26%), Positives = 25/67 (37%), Gaps = 15/67 (22%)

Query: 102 RGETILKALERIAFE---EFQLNSLHLEVMENNFKAIAFYEKNHYELEGRLKGFISKEKE 158
R + + AL A E E L LE + N A FY K+H+ +
Sbjct: 102 RKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA----------- 150

Query: 159 FIDVFLY 165
+D LY
Sbjct: 151 -VDTMLY 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10375PREPILNPTASE270.039 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 27.1 bits (60), Expect = 0.039
Identities = 17/59 (28%), Positives = 25/59 (42%), Gaps = 8/59 (13%)

Query: 32 FVIVAWLFRF--KSIAFSILITLLVILVDIWVYSDVRQFLL-DTASSPILLLVALLIKW 87
V VA ++A +L +LV L I D+ + LL D + P+L LL
Sbjct: 121 SVAVAMTLAPGWGTLAALLLTWVLVALTFI----DLDKMLLPDQLTLPLLWG-GLLFNL 174


6K747_10975K747_11060Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_10975-213-3.165319membrane protein
K747_10980-213-2.766374hypothetical protein
K747_10990114-0.044463GTP-binding protein
K747_10995515-0.935883hypothetical protein
K747_11000114-1.017928type II restriction endonuclease
K747_11005215-0.708557non-functional type II restriction endonuclease
K747_11010215-0.276536catalase
K747_11015316-0.420504membrane protein
K747_11020314-1.567680membrane protein
K747_110252150.006035hypothetical protein
K747_11030-1121.603400hypothetical protein
K747_11035-1121.696260hypothetical protein
K747_110400122.028603nuclease
K747_110450111.996532Holliday junction resolvase
K747_110500131.928356membrane protein
K747_11060219-0.444944Tat pathway signal protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10990TCRTETOQM1981e-57 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 198 bits (504), Expect = 1e-57
Identities = 115/461 (24%), Positives = 190/461 (41%), Gaps = 67/461 (14%)

Query: 3 NIRNIAVIAHVDHGKTTLVDGLLSQSGTFSEREKVDE--RVMDSNDLERERGITILSKNT 60
I NI V+AHVD GKTTL + LL SG +E VD+ D+ LER+RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 61 AIYYKDTKINIIDTPGHADFGGEVERVLKMVDGVLLLVDAQEGVMPQTKFVVKKALSFGI 120
+ +++TK+NIIDTPGH DF EV R L ++DG +LL+ A++GV QT+ + GI
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 121 CPIVVVNKIDKPAAEPDRVVDEVFDLF---------VAMGASDKQLDFPV-----VYAAA 166
I +NKID+ + V ++ + V + + +F
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 167 RDGYAMKSLDDE----------------------------KKNL--EPLFETILEHVPSP 196
D K + + K N+ + L E I S
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSS 241

Query: 197 SGSVDEPLQMQIFTLDYDNYVGKIGIARVFNGSVKKNESVLLMKSDGSKENGRITKLIGF 256
+ L ++F ++Y ++ R+++G + +SV + KE +IT++
Sbjct: 242 THRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRI----SEKEKIKITEMYTS 297

Query: 257 LGLARTEIENAYAGDIVAIAG--FSAMDV-GDSVVDPTNPMPLDPMHLEEPTMSVYFAVN 313
+ +I+ AY+G+IV + V GD+ + P +P P + +
Sbjct: 298 INGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENP----LPLLQTTVEPS 353

Query: 314 DSPLAGLEGKHVTANKLKDRLLKEMQTNIAMKCEEMGEGKFKVSGRGELQITILAENLRR 373
+ + D LL+ + + +S G++Q+ + L+
Sbjct: 354 KPQQREMLLDALLEISDSDPLLRYYVDSAT--------HEIILSFLGKVQMEVTCALLQE 405

Query: 374 E-GFEFSISRPEVIIKEENGVKCEPFEHLVIDTPQDFSGAI 413
+ E I P VI E K E H+ + P F +I
Sbjct: 406 KYHVEIEIKEPTVIYMERPLKKAEYTIHIEVP-PNPFWASI 445



Score = 41.8 bits (98), Expect = 8e-06
Identities = 20/80 (25%), Positives = 30/80 (37%), Gaps = 1/80 (1%)

Query: 396 EPFEHLVIDTPQDFSGAIIERLGKRKAEMKAMNPMSDGYTRLEFEIPARGLIGYRSEFLT 455
EP+ I PQ++ K A + + + L EIPAR + YRS+
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCIQEYRSDLTF 595

Query: 456 DTKGEGVMNHSFLEFRPFSG 475
T G V + +G
Sbjct: 596 FTNGRSVCLTELKGYHVTTG 615


7K747_07865K747_07530Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_07865213-2.669005ribosomal protein S12 methylthiotransferase
K747_07860218-4.115055GTPase
K747_11535316-3.615868dynamin family protein
K747_11540213-2.297078hypothetical protein
K747_07850-112-1.601001hypothetical protein
K747_07845-1130.098834hypothetical protein
K747_07840-1121.168126ATP-binding protein
K747_078351142.188090tRNA(Ile)-lysidine synthetase
K747_078302132.585082tRNA-dihydrouridine synthase
K747_078251102.031272membrane protein
K747_078101122.050098C4-dicarboxylate ABC transporter
K747_078050121.276828L-asparaginase
K747_07800112-0.812510zinc ABC transporter substrate-binding protein
K747_07790214-1.213305lysE type translocator family protein
K747_07785114-1.170570DNA polymerase III subunit gamma/tau
K747_07780-112-1.404977P-loop hydrolase
K747_07775011-1.760819lipopolysaccharide ABC transporter ATP-binding
K747_07770-111-2.829016RNA polymerase sigma54 factor
K747_07765-215-3.516002hypothetical protein
K747_07760016-3.228407membrane protein
K747_07755317-2.130591competence protein ComGF
K747_07750925-2.395646hypothetical protein
K747_11545926-1.890171hypothetical protein
K747_07745824-1.807653hypothetical protein
K747_07740724-1.110126hypothetical protein
K747_07735724-1.588209hypothetical protein
K747_07730625-3.289258hypothetical protein
K747_07725525-3.965152hypothetical protein
K747_07720525-4.960491portal protein
K747_11550929-7.428523hypothetical protein
K747_07705827-6.635488hypothetical protein
K747_07700930-5.013262hypothetical protein
K747_076951132-3.552791holin
K747_076901231-3.552791hypothetical protein
K747_076851234-2.735238DNA methyltransferase
K747_076801335-3.539699hypothetical protein
K747_076751433-3.730816hypothetical protein
K747_07670931-3.630680hypothetical protein
K747_076651026-5.188729hypothetical protein
K747_11555622-2.787426hypothetical protein
K747_11560621-3.027176hypothetical protein
K747_11565317-1.752577hypothetical protein
K747_07645016-0.942990hypothetical protein
K747_07640012-0.247826terminase
K747_07635-1130.292272acetolactate synthase
K747_07630090.268324hypothetical protein
K747_07625-1100.68970016S rRNA methyltransferase
K747_07620-1110.218049membrane protein
K747_076100110.256674ATPase AAA
K747_076052111.379425hypothetical protein
K747_076001111.582645DNA gyrase subunit A
K747_075951131.724908diacylglycerol kinase
K747_075902132.112358hypothetical protein
K747_075853133.147173acetone carboxylase subunit gamma
K747_075802133.614547acetone carboxylase subunit alpha
K747_075700132.601669membrane protein
K747_07565-2122.180903short-chain fatty acid transporter
K747_075601121.507418succinyl-CoA:3-ketoacid-CoA transferase
K747_075550110.981700succinyl-CoA:3-ketoacid-CoA transferase
K747_075500100.881379acetyl-CoA acetyltransferase
K747_075451110.222430polysaccharide biosynthesis protein
K747_075403120.884057iron transporter
K747_075306161.216138ligand-gated channel
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07860SECA300.027 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 30.2 bits (68), Expect = 0.027
Identities = 21/134 (15%), Positives = 51/134 (38%), Gaps = 24/134 (17%)

Query: 343 RAKIMKSNCNKALKVVEQLQKAIEITIEKRIDPMIKEAQEHQQEARYNLDRSTEKFILNL 402
R +++ + + + + + + TI+ I P + +E +R F L+L
Sbjct: 663 RNELLDVS-DVSETINSIREDVFKATIDAYIPP--QSLEEMWDIPGLQ-ERLKNDFDLDL 718

Query: 403 TNSAFYEIDQ--FKSDLREKMYAHINKNIEDEE-------CKEIFKNELIQGI------- 446
+ + + + + LRE++ A + + +E + K ++Q +
Sbjct: 719 PIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEH 778

Query: 447 ----ETLHEGIKWR 456
+ L +GI R
Sbjct: 779 LAAMDYLRQGIHLR 792


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07725TYPE4SSCAGX280.011 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 28.2 bits (62), Expect = 0.011
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 1/42 (2%)

Query: 92 EQNKNAKESMRMAKEQFE-LEKQRYNAREQERLKNREAIDNI 132
E+ K A E + AKEQ + +K + R++ER KNR ++N+
Sbjct: 142 EEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANLENL 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07690FbpA_PF05833290.031 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 28.7 bits (64), Expect = 0.031
Identities = 21/115 (18%), Positives = 46/115 (40%), Gaps = 4/115 (3%)

Query: 26 IYQISNVLNQLDLLKNFSKHDLEIVGKIQRLKSALKN---YETSEQELKAKIDALKQRLE 82
IY + L+ ++L +S++ + + K+ +N Y +LK +A ++L
Sbjct: 346 IYALKKGLSHIELANYYSENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAANEQLL 405

Query: 83 AKKQELEARLNMELQSARVSEIQKLNDAGNEL-KTNLINKLKEAKDNLAQALNPQ 136
++EL ++ ++ + EL +T I K K ++ P
Sbjct: 406 QNEEELNYLYSVLTNINNADNYDEIEEIKKELIETGYIKFKKIYKSKKSKTSKPM 460


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07675RTXTOXIND290.028 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.6 bits (64), Expect = 0.028
Identities = 31/173 (17%), Positives = 64/173 (36%), Gaps = 18/173 (10%)

Query: 8 RNFILFKDALQKQNFNNKDLNTTSMQAALQSEQLALSEEAQGGQSEQVRAKMQIDFLVMQ 67
+ D QN + +++ +L EQ + + + Q Q E K + + L +
Sbjct: 165 LPELKLPDEPYFQNVSEEEV---LRLTSLIKEQFS-TWQNQKYQKELNLDKKRAERLTVL 220

Query: 68 ANLQNEKAETLNKLIQCQ-AMLKSLKDNAMINRANALVSLLQ-VQANAANGITASNFDIT 125
A + + + ++ + + SL I + L + V+A + S
Sbjct: 221 ARINRYENLS--RVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQ---- 274

Query: 126 FKLISQIGSEYNQIKFSTGGIISVQEKEQTNELKTI---LNNLSKELEKLNEQ 175
+ QI SE K + + + E ++L+ + L+ EL K E+
Sbjct: 275 ---LEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEER 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07610HTHFIS395e-137 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 395 bits (1016), Expect = e-137
Identities = 128/384 (33%), Positives = 198/384 (51%), Gaps = 9/384 (2%)

Query: 2 KIAIVEDDINMRKSLELFFELQDDLEIVSFKNPKDALVKL-DESFDLVITDINMPHMDGL 60
I + +DD +R L + ++ N + DLV+TD+ MP +
Sbjct: 5 TILVADDDAAIRTVLNQALS-RAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 61 EFLRLLEGKYES---IVITGNATLNKAIDSIRLGVKDFFQKPFKPELLLESIYRTKKVLE 117
+ L ++ +V++ T AI + G D+ KPF E I + L
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT---ELIGIIGRALA 120

Query: 118 FQKKHPLEKPLKKPHKHSFLAASKALEESKRQALKVASTDANVMLLGESGVGKEVFAHFI 177
K+ P + + S A++E R ++ TD +M+ GESG GKE+ A +
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180

Query: 178 HQHSQRSKHPFIAINMSAIPEHLLESELFGYQKGAFTDATAPKMGLFESAHKGTIFLDEI 237
H + +R PF+AINM+AIP L+ESELFG++KGAFT A G FE A GT+FLDEI
Sbjct: 181 HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI 240

Query: 238 AEMPLQLQSKLLRVVQEKEITRLGDNKSVKIDVRFISATNANMKEKIAAKEFREDLFFRL 297
+MP+ Q++LLRV+Q+ E T +G ++ DVR ++ATN ++K+ I FREDL++RL
Sbjct: 241 GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300

Query: 298 QIVPIVIAPLRERIEEILPIAEIKLKEVCDAYHLGPKSFSKNAAKRLLEYSWHGNVRELL 357
+VP+ + PLR+R E+I + +++ L K F + A + + + W GNVREL
Sbjct: 301 NVVPLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALELMKAHPWPGNVRELE 359

Query: 358 GVVERAAILSEGAEIQEKDLFLER 381
+V R L I + + E
Sbjct: 360 NLVRRLTALYPQDVITREIIENEL 383


8K747_06910K747_06670Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_06910212-1.155554Fe-S oxidoreductase
K747_069002140.018044diaminopimelate epimerase
K747_068953150.021639membrane protein
K747_11640317-0.343290hypothetical protein
K747_068852141.703954hypothetical protein
K747_068802172.65130330S ribosomal protein S21
K747_068752172.5987963-ketoacyl-ACP reductase
K747_068702181.753040acyl carrier protein
K747_068650140.863999hypothetical protein
K747_06860-2120.8921483-oxoacyl-ACP synthase
K747_06855-214-0.722062acetyl-CoA carboxylase subunit alpha
K747_116450110.093712hypothetical protein
K747_06850-2100.479499membrane protein
K747_11650-3110.598960hypothetical protein
K747_06835015-0.28885516S rRNA methyltransferase
K747_06830216-0.61165950S ribosomal protein L31
K747_06825317-0.394642transcription termination factor Rho
K747_06820521-1.868150glutamate racemase
K747_06815621-2.667243transposase
K747_06810721-3.337857cytochrome C oxidase subunit II
K747_06805722-4.225766cag pathogenicity island protein
K747_06800519-3.182851sodium:calcium antiporter
K747_06795419-3.174310sodium:calcium antiporter
K747_06790419-2.932075transporter
K747_06785517-3.419540sodium:calcium antiporter
K747_06780517-2.534092sodium:calcium antiporter
K747_06775618-2.688669sodium:calcium antiporter
K747_06770619-4.380551sodium:calcium antiporter
K747_067651023-6.114655sodium:calcium antiporter
K747_067551326-5.648237sodium:calcium antiporter
K747_067501331-5.309678hypothetical protein
K747_116551228-5.275326cag pathogenicity island protein
K747_067351327-5.646408hypothetical protein
K747_067301426-5.027447cag pathogenicity island protein
K747_067251423-4.876764cag pathogenicity island protein
K747_067201020-3.444695cag pathogenicity island protein
K747_067151120-3.373385cag pathogenicity island protein
K747_116601119-3.407120hypothetical protein
K747_116651118-3.106059cagX protein
K747_116701118-2.952750hypothetical protein
K747_116751018-2.085204hypothetical protein
K747_066951121-3.240649hypothetical protein
K747_066901121-3.073292sodium:calcium antiporter
K747_06685720-2.655319hypothetical protein
K747_06675315-2.275574sodium:calcium antiporter
K747_06670213-1.996325sodium:calcium antiporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06875DHBDHDRGNASE1247e-37 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (313), Expect = 7e-37
Identities = 78/250 (31%), Positives = 112/250 (44%), Gaps = 11/250 (4%)

Query: 5 GKNVLITGASKGIGAEIARTLASMGLKVWINYRSNAEVADALKNELEEKGYKAAVIKFDA 64
GK ITGA++GIG +ARTLAS G + N E + + + L+ + A D
Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHI-AAVDYNPEKLEKVVSSLKAEARHAEAFPADV 66

Query: 65 ASESGFIEAIQTIVQSDGGLSYLVNNAGVVRDKLAIKMKTEDFHHVIDNNLTSAFIGCRE 124
+ E I + G + LVN AGV+R L + E++ N T F R
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 125 ALKVMSKSRFGSVVNIASIIGERGNMGQTNYSASKGGMIAMSKSFAYEGALRNIRFNSVT 184
K M R GS+V + S Y++SK + +K E A NIR N V+
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 185 PGFIETDMNANL--KDELKADYVK--------NIPLNRLGSAKEVAEVVAFLLSDHSSYI 234
PG ETDM +L + +K IPL +L ++A+ V FL+S + +I
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 235 TGETLKVNGG 244
T L V+GG
Sbjct: 247 TMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06830PF01206270.004 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 26.6 bits (59), Expect = 0.004
Identities = 7/22 (31%), Positives = 12/22 (54%)

Query: 19 SGKEIEVLSTKPEMRIDISSFC 40
+G+ + V++T P D SF
Sbjct: 31 AGEVLYVMATDPGSVKDFESFS 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06810TYPE4SSCAGA17100.0 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 1710 bits (4430), Expect = 0.0
Identities = 921/1191 (77%), Positives = 1018/1191 (85%), Gaps = 59/1191 (4%)

Query: 1 MTNETIDQTTTPDQTPNQTDFVPQRFINNLQVAFIKVDSAVASFDPDQKPIVDKNDRDNR 60
MTNETIDQ Q + F PQ+FINNLQVAF+KVD+AVAS+DPDQKPIVDKNDRDNR
Sbjct: 1 MTNETIDQ-----QPQTEAAFNPQQFINNLQVAFLKVDNAVASYDPDQKPIVDKNDRDNR 55

Query: 61 QAFEKISQLREEYANKAIKNPAKKNQYFSDFINKSNDLINKDNLIDVDSSVESFKKFGDQ 120
QAFE ISQLREEY+NKAIKNP KKNQYFSDFINKSNDLINKDNLIDV+SS +SF+KFGDQ
Sbjct: 56 QAFEGISQLREEYSNKAIKNPTKKNQYFSDFINKSNDLINKDNLIDVESSTKSFQKFGDQ 115

Query: 121 RYQIFTSWVSLQKDPSEINTQQIRNFMENIIQPPISDDKEKAEFLRSAKQSFAGIIIGNQ 180
RY+IFTSWVS Q DPS+INT+ IRNFMENIIQPPI DDKEKAEFL+SAKQSFAGIIIGNQ
Sbjct: 116 RYRIFTSWVSHQNDPSKINTRSIRNFMENIIQPPILDDKEKAEFLKSAKQSFAGIIIGNQ 175

Query: 181 IRSDQKFMGVFDESLKERQEAEKNAEPAGGDWLDIFLSFVFNKKQSSDLKETLHQEPRPD 240
IR+DQKFMGVFDESLKERQEAEKN EP GGDWLDIFLSF+F+KKQSSD+KE ++QEP P
Sbjct: 176 IRTDQKFMGVFDESLKERQEAEKNGEPTGGDWLDIFLSFIFDKKQSSDVKEAINQEPVPH 235

Query: 241 FEQNLATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADKNPNYKFNQLLIH 300
+ ++ATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVAD +PNYKFNQLLIH
Sbjct: 236 VQPDIATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKFNQLLIH 295

Query: 301 NNALSSVLMGGHSNIEPEKVSLLYGDNGGPEARHDWNATVGYKNQQGSNVATLINAHLNN 360
NNALSSVLMG H+ IEPEKVSLLYG NGGP ARHDWNATVGYK+QQG+NVAT+IN H+ N
Sbjct: 296 NNALSSVLMGSHNGIEPEKVSLLYGGNGGPGARHDWNATVGYKDQQGNNVATIINVHMKN 355

Query: 361 GSGLIIAGNENGIKNPSFYLYKEDQLTGLKQAMSQEEIQNKVDFMEFLAKNNAKLDNLSE 420
GSGL+IAG E GI NPSFYLYKEDQLTG ++A+SQEEIQNK+DFMEFLA+NNAKLDNLSE
Sbjct: 356 GSGLVIAGGEKGINNPSFYLYKEDQLTGSQRALSQEEIQNKIDFMEFLAQNNAKLDNLSE 415

Query: 421 KEKEKFQTEIGNFQKDRKAYLDALGNDHIAFVSKKDPKHLALVTEFGNGEVSYTLKDYGK 480
KEKEKF+TEI +FQKD KAYLDALGND IAFVSKKD KH AL+TEFGNG++SYTLKDYGK
Sbjct: 416 KEKEKFRTEIKDFQKDSKAYLDALGNDRIAFVSKKDTKHSALITEFGNGDLSYTLKDYGK 475

Query: 481 KQDKALDGEVKTTLQGSLKYDGVMFVDYSNFKYTNASKSPDKGVGATNGVSHLEANFSKV 540
K DKALD E TLQGSLK+DGVMFVDYSNFKYTNASK+P+KGVG TNGVSHLE F+KV
Sbjct: 476 KADKALDREKNVTLQGSLKHDGVMFVDYSNFKYTNASKNPNKGVGVTNGVSHLEVGFNKV 535

Query: 541 AVFNLPNLNNLAITNHIRRDLEDKLLAKGLSPQEANKLIKDFLNSNKEMVGKVSNFNKAV 600
A+FNLP+LNNLAIT+ +RR+LEDKL KGLSPQEANKLIKDFL+SNKE+VGK NFNKAV
Sbjct: 536 AIFNLPDLNNLAITSFVRRNLEDKLTTKGLSPQEANKLIKDFLSSNKELVGKTLNFNKAV 595

Query: 601 AEAKNTGNYDEVKKAQKDLEKSIRKREHLEKEVVKKLESRNDNKNRMEAKAQANSQKDKI 660
A+AKNTGNYDEVKKAQKDLEKS+RKREHLEKEV KKLES++ NKN+MEAKAQANSQKD+I
Sbjct: 596 ADAKNTGNYDEVKKAQKDLEKSLRKREHLEKEVEKKLESKSGNKNKMEAKAQANSQKDEI 655

Query: 661 FALINKEASKEARAAAFDPNLKGIRSELSDKLENINKNLKDFGKSFDDFKNGKNNDFSKA 720
FALINKEA+++ARA A+ NLKGI+ ELSDKLEN+NKNLKDF KSFD+FKNGKN DFSKA
Sbjct: 656 FALINKEANRDARAIAYAQNLKGIKRELSDKLENVNKNLKDFDKSFDEFKNGKNKDFSKA 715

Query: 721 EETLKALKDLVKDLGINPEWISKIENLNAALNDFKNGKNKDFSKVTQAKSDLENSIKDVI 780
EETLKALK VKDLGINPEWISK+ENLNAALN+FKNGKNKDFSKVTQAKSDLENS+KDVI
Sbjct: 716 EETLKALKGSVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSDLENSVKDVI 775

Query: 781 INQKITDKVDNLNQAVSETKLTGDFSKVEQALAELKNLS-------------LDLGKNSD 827
INQK+TDKVDNLNQAVS K TGDFS+VEQALA+LKN S L+ K S+
Sbjct: 776 INQKVTDKVDNLNQAVSVAKATGDFSRVEQALADLKNFSKEQLAQQAQKNESLNARKKSE 835

Query: 828 LQKSVKNGVNGTLVGNGLSKTEATTLTKNFSDIRKELNEKLFGNSNNNNNGLKNNTEPIY 887
+ +SVKNGVNGTLVGNGLS+ EATTL+KNFSDI+KELN KL +NNNNNGLKN EPIY
Sbjct: 836 IYQSVKNGVNGTLVGNGLSQAEATTLSKNFSDIKKELNAKLGNFNNNNNNGLKN--EPIY 893

Query: 888 AQVNKKKTGQVASPEESIYAQVAKKVSAKIDQLNEATSAINRKIDRINKIASAGKGVGGF 947
A+VNKKK GQ AS EE IYAQVAKKV+AKID+LN+ S G GV G
Sbjct: 894 AKVNKKKAGQAASLEEPIYAQVAKKVNAKIDRLNQIAS---------------GLGVVG- 937

Query: 948 SGAGRSASPEPIYATIDFDEANQAGFPLRRSAAVNDLSKVGLSREQELTRRIGDLNQAVS 1007
AGFPL+R V+DLSKVGLSR QEL ++I +LNQAVS
Sbjct: 938 ---------------------QAAGFPLKRHDKVDDLSKVGLSRNQELAQKIDNLNQAVS 976

Query: 1008 EAKTGHFDNLEQKIDELKDSTKKNALKLFIESAKQVPTGLQAKLDNYAANSHTRINSNVR 1067
EAK G F NLEQ ID+LKDSTK N + L++ESAK+VP L AKLDNYA NSH RINSN++
Sbjct: 977 EAKAGFFGNLEQTIDKLKDSTKHNPMNLWVESAKKVPASLSAKLDNYATNSHIRINSNIK 1036

Query: 1068 NGAINERATGMLTQKNPEWLKLVNNKIVAHNVGSAHLSEYDKIGFNQKNMKDYSDSFKFS 1127
NGAINE+ATGMLTQKNPEWLKLVN+KIVAHNVGS LSEYDKIGFNQKNMKDYSDSFKFS
Sbjct: 1037 NGAINEKATGMLTQKNPEWLKLVNDKIVAHNVGSVPLSEYDKIGFNQKNMKDYSDSFKFS 1096

Query: 1128 TKLNNAVKDIKSNFVQFLTNTFSTGS-YSLMKANVEHGVKNT-TKSGFQKS 1176
TKLNNAVKD S F QFLTN FST S Y L + N EHG+KN TK GFQKS
Sbjct: 1097 TKLNNAVKDTNSGFTQFLTNAFSTASYYCLARENAEHGIKNVNTKGGFQKS 1147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06790ACRIFLAVINRP320.012 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 32.1 bits (73), Expect = 0.012
Identities = 21/88 (23%), Positives = 32/88 (36%), Gaps = 18/88 (20%)

Query: 19 EVQKRQFQKIEELKADMQKGVNPFFKVLFDGGNRLFGFPETFIYSSI-------FILFVT 71
+ K K+ EL+ +G+ +D F+ SI F +
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMK--VLYPYD--------TTPFVQLSIHEVVKTLFEAIML 350

Query: 72 IVLSVILF-QAYEPVLIVAIVVVLVALG 98
+ L + LF Q LI I V +V LG
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLG 378


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06715PF043351195e-35 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 119 bits (299), Expect = 5e-35
Identities = 44/205 (21%), Positives = 74/205 (36%), Gaps = 10/205 (4%)

Query: 27 KLNKANRTFKRAFYL---SMALNIAAVTSIVMMMPLKKTDIFVYGIDRYTGEFKIVKRSD 83
KL A R+ K A+ + + AL A V ++ + PLK + +V +DR TGE I +
Sbjct: 24 KLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLH 83

Query: 84 A-RQIVNSEAVVDSATSKFVSLLFGYSKNSLRDRKDQLMQYCDVSFQTQAMRMFNENIRQ 142
I EAV + +V G+ + + D +M Q + R + + Q
Sbjct: 84 GDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDNPQ 143

Query: 143 FVDKVRA-EAIISSNIQREKVKNSPLTRLTFFITIKITPDTMENYEYITKKQVTIYYDFA 201
+ A + I + +F +T T TI Y
Sbjct: 144 SPQNILANRTDVFVEI-KRVSFLGGNVAQVYFTKESVTGSNS----TKTDAVATIKYKVD 198

Query: 202 RGNSSQENLIINPFGFKVFDIQITD 226
S + + NP G++V +
Sbjct: 199 GTPSKEVDRFKNPLGYQVESYRADV 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11660TYPE4SSCAGX2662e-90 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 266 bits (680), Expect = 2e-90
Identities = 143/154 (92%), Positives = 145/154 (94%), Gaps = 1/154 (0%)

Query: 1 MEQAFFKKIVGCFCLGYLFLSSVIEAAP-DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 59
M QAFFKKIVGCFCLGYLFLSS IEA DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS
Sbjct: 1 MGQAFFKKIVGCFCLGYLFLSSAIEAVALDIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 60

Query: 60 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 119
LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR
Sbjct: 61 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 120

Query: 120 DYQEFLKTKKLIVDAPDPKELEEQKKSSRKRKRS 153
DYQEFLKTKKLIVDAPDPKELEEQKK+ K K +
Sbjct: 121 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEA 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11665TYPE4SSCAGX5570.0 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 557 bits (1435), Expect = 0.0
Identities = 333/336 (99%), Positives = 335/336 (99%)

Query: 1 MSNPQNLSNNKNLSEFIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVKQ 60
MSNPQNLSNNKNLSE IKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAV+Q
Sbjct: 187 MSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVRQ 246

Query: 61 RAKDKINIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAY 120
RAKDKI+IKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAY
Sbjct: 247 RAKDKISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAY 306

Query: 121 LTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKE 180
LTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKE
Sbjct: 307 LTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKE 366

Query: 181 KIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFT 240
KIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFT
Sbjct: 367 KIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFT 426

Query: 241 YFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVT 300
YFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVT
Sbjct: 427 YFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVT 486

Query: 301 VINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 336
VINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK
Sbjct: 487 VINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 522


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11675IGASERPTASE422e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 42.4 bits (99), Expect = 2e-05
Identities = 40/259 (15%), Positives = 85/259 (32%), Gaps = 36/259 (13%)

Query: 118 TPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDC 177
P ++ + + ++KT + ++ T + ++ +EAK +VKA
Sbjct: 1023 APVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQT 1082

Query: 178 VSQAKTEAEKKECEKLLTPEAKKLLEQQALDCLKSAKTDEERKKCLKDLPKDLQKKVLAK 237
A++ +E KE + T E A ++E K AK
Sbjct: 1083 NEVAQSGSETKETQTTETKET--------------ATVEKEEK---------------AK 1113

Query: 238 ESLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKE 297
+ ++ KQE + + P+A+ E + ++K SQ T A+ ++
Sbjct: 1114 VETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEP---QSQTNTTADTEQ 1170

Query: 298 CEKLLTPEAKKLLEQQALDCLKSAKTDEERKKCLKDLPKDLQKKVLAKESLKAYKDCVSR 357
K + ++ + + + + P Q V ++ S K
Sbjct: 1171 PAKETSSNVEQPVTEST----TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRS 1226

Query: 358 ARNEKEKQECEKLLTPEAK 376
R+ E + +
Sbjct: 1227 VRSVPHNVEPATTSSNDRS 1245



Score = 36.6 bits (84), Expect = 9e-04
Identities = 35/269 (13%), Positives = 76/269 (28%), Gaps = 18/269 (6%)

Query: 357 RARNEKEKQECEKLLTPEAKKLLEEAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKKL 416
+ + + TP + + S +R + + +
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEI---ARVDEAPVPPPAPATPSETTETV 1040

Query: 417 LEEAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKNLLEQQALDCLKSAKTEAEKKRCV 476
E +K+ K + A E Q E ++ Q + +S E +
Sbjct: 1041 AENSKQESKTVEKNEQDAT-ETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTE 1099

Query: 477 KDLPKDLQKKVLAKESVKAYLDCVSRARNEKEKKECEKLLTPEAKKLLEEAKESLKAYKD 536
++K+ AK + + K+E + + P+A+ E
Sbjct: 1100 TKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVN----- 1154

Query: 537 CLSQARNEEERRACEKLLTPEARKLLEQEVKKSVKAYLDCVSRARNEKEKQECEKLLTPE 596
+ + +++ A + E +EQ V + N E + TP
Sbjct: 1155 -IKEPQSQTNTTADTEQPAKETSSNVEQPVTE------STTVNTGNSVV--ENPENTTPA 1205

Query: 597 ARKFLAKQVLSCLEKARNEEERKACLKNI 625
+ S K R+ ++ N+
Sbjct: 1206 TTQPTVNSESSNKPKNRHRRSVRSVPHNV 1234



Score = 35.4 bits (81), Expect = 0.002
Identities = 35/242 (14%), Positives = 86/242 (35%), Gaps = 5/242 (2%)

Query: 65 KNAKTDEERKKCLKDLPKDLQSDILAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKKL 124
+ ++ E + + P + ++ + + + EK E + T +
Sbjct: 1008 PSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNRE 1067

Query: 125 LEEEAKESVKAYLDC--VSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDCVSQAK 182
+ +EAK +VKA V+Q+ +E ++ + + +K E+AK + +
Sbjct: 1068 VAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS 1127

Query: 183 TEAEKKECEKLLTPEAKKLLEQQALDCLKSAKTDEERKKCLKDLPKDLQKKV---LAKES 239
+ K+E + + P+A+ E +K ++ + K+ V + + +
Sbjct: 1128 QVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTEST 1187

Query: 240 LKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECE 299
+ V + + E+ + + SV++ V A T + +
Sbjct: 1188 TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTV 1247

Query: 300 KL 301
L
Sbjct: 1248 AL 1249



Score = 33.5 bits (76), Expect = 0.007
Identities = 23/160 (14%), Positives = 53/160 (33%), Gaps = 2/160 (1%)

Query: 264 TPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKLLEQQALDCLKSAKT 323
TP E + ++ Q TE + E ++ Q + +S
Sbjct: 1032 TPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE 1091

Query: 324 DEERKKCLKDLPKDLQKKVLAKESLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEAK 383
+E + ++K+ AK + ++ KQE + + P+A+ E
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREND- 1150

Query: 384 KSLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEAKKS 423
K+ S+ + ++ K + ++ + E+
Sbjct: 1151 -PTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTV 1189



Score = 32.3 bits (73), Expect = 0.014
Identities = 32/239 (13%), Positives = 80/239 (33%), Gaps = 8/239 (3%)

Query: 34 KARNEKEKKECEKLLTPEAKKKLEQQVLDCLKNAKTDEERKKCLKDLPKD--LQSDILAK 91
+ NE+ + E + P A + +N+K + + + + + Q+ +AK
Sbjct: 1011 PSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAK 1070

Query: 92 KSLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKE 151
++ K + E ++ T E K+ E +E K + + +
Sbjct: 1071 EAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVS 1130

Query: 152 CEKLLTPEAKKKLEEAKKSVKAYL--DCVSQAKTEAEKKECEKLLTPEAKKLLEQQALDC 209
++ + + + E A+++ + SQ T A+ ++ K + ++ + +
Sbjct: 1131 PKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTEST--- 1187

Query: 210 LKSAKTDEERKKCLKDLPKDLQKKVLAKESLKAYKDCVSRARNEKEKQECEKLLTPEAK 268
+ + P Q V ++ S K R+ E + +
Sbjct: 1188 -TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS 1245



Score = 31.6 bits (71), Expect = 0.028
Identities = 25/157 (15%), Positives = 60/157 (38%), Gaps = 5/157 (3%)

Query: 16 QKELLADMSVKAYKDCVSKARNEKEKKECEKLLTPEAKKKLE--QQVLDCLKNAKTDEER 73
E +A+ S + K ++ E + + EAK ++ Q + ++ +E
Sbjct: 1036 TTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKET 1095

Query: 74 KKCLKDLPKDLQSDILAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESV 133
+ ++ + AK + ++ KQE + + P+A+ E + ++
Sbjct: 1096 QTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNI 1155

Query: 134 KAYLDCVSQAKTEAEKKECEKLLTPEAKKKLEEAKKS 170
K SQ T A+ ++ K + ++ + E+
Sbjct: 1156 KEP---QSQTNTTADTEQPAKETSSNVEQPVTESTTV 1189


9K747_06605K747_06435Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_06605-116-3.120957dihydrodipicolinate reductase
K747_06595-211-2.372737plasmid stabilization protein
K747_06590-210-2.600683UDP-sugar diphosphatase
K747_06585-210-1.936345membrane protein
K747_06580-312-2.262453restriction endonuclease R.HpyAXII
K747_06575-211-1.617397adenine methyltransferase M.HpyAXII
K747_06570-313-1.150974DNA gyrase subunit B
K747_06560012-2.243788DNA polymerase III subunit beta
K747_11680213-1.556250phospholipase
K747_06545113-1.812393sodium- and chloride-dependent transporter
K747_06540010-1.235676sodium- and chloride-dependent transporter
K747_06530011-1.136449hypothetical protein
K747_06525013-1.446163UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
K747_06520114-0.908988phospho-N-acetylmuramoyl-pentapeptide-
K747_06510213-1.47749750S ribosomal protein L28
K747_06505111-1.554726potassium channel protein
K747_06500-111-0.439286hypothetical protein
K747_06495-110-0.361833hypothetical protein
K747_06490-2100.926400Holliday junction DNA helicase RuvA
K747_06485-1101.053345hypothetical protein
K747_064750121.580613cysteinyl-tRNA synthetase
K747_064701121.764938toxin
K747_064650153.214791iron ABC transporter ATP-binding protein
K747_064601163.336766iron ABC transporter permease
K747_064501161.900051VdlD
K747_064450172.325791addiction module toxin RelE
K747_064400182.703802hypothetical protein
K747_064350173.025454membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06595IGASERPTASE442e-06 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 43.5 bits (102), Expect = 2e-06
Identities = 33/194 (17%), Positives = 64/194 (32%), Gaps = 23/194 (11%)

Query: 246 RIKKQEEKVDAREI----KREIKQEAIKEPKKANQGTENAPTLEEKNYQKAERKLDVKEE 301
++K+ + VD I + ++ + + AP +E V E
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAEN 1043

Query: 302 RRRLRDERKKANATKKAMEFEEREKTHDERDERETEERRKALGMDQGNEKANAKENEQEI 361
++ + + + A E + + + + + Q NE A + +E
Sbjct: 1044 SKQ--ESKTVEKNEQDATETTAQNREVAKEAKSNVKANT------QTNEVAQSGSETKET 1095

Query: 362 KQEATKEPSNENNATQQGENKPNSKEEKRRLKEEKKKA---KAEQRAREFEQRAKEHQ-E 417
+ TKE AT + E K +K E + +E K +Q E Q E E
Sbjct: 1096 QTTETKET-----ATVEKEEK--AKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARE 1148

Query: 418 RDEKELEERRKALE 431
D + ++
Sbjct: 1149 NDPTVNIKEPQSQT 1162



Score = 36.6 bits (84), Expect = 2e-04
Identities = 30/164 (18%), Positives = 57/164 (34%), Gaps = 19/164 (11%)

Query: 270 EPKKANQGTENAPTLE---EKNYQKAERKLDV-----KEERRRLRDERKKANATKKAMEF 321
+ EN+ EKN Q A KE + ++ + + E
Sbjct: 1033 PSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSET 1092

Query: 322 EEREKTHDERDERETEERRKALGMDQGNEKANAKENEQEIKQEATKEPSNENNATQQGEN 381
+E + T + ++ E+ KA K ++ ++ K + P E + T Q +
Sbjct: 1093 KETQTT-ETKETATVEKEEKA--------KVETEKTQEVPKVTSQVSPKQEQSETVQPQA 1143

Query: 382 KPNSKEEKRRLKEEKKKAKAEQRAREFEQRAKEHQERDEKELEE 425
+P + + +E + EQ AKE E+ + E
Sbjct: 1144 EPARENDPTVNIKE--PQSQTNTTADTEQPAKETSSNVEQPVTE 1185


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11680PHPHLIPASEA12622e-88 Bacterial phospholipase A1 protein signature.
		>PHPHLIPASEA1#Bacterial phospholipase A1 protein signature.

Length = 289

Score = 262 bits (670), Expect = 2e-88
Identities = 73/279 (26%), Positives = 117/279 (41%), Gaps = 48/279 (17%)

Query: 77 YLGTYFLPFYHS-FTPIFQWYHPNINPYQRNEFKFQISFRVPVFRHILWTKGTLYLAYTQ 135
Y Y + S + +++E KFQ+S P++R IL L +YTQ
Sbjct: 55 YDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGILGPNSVLGASYTQ 114

Query: 136 TDWFQIYNNPQSAPMRMINFMPELIYVYPINFKPFGGKIGNFSEIWIGWQHISNGVGGAQ 195
W+Q+ N+ +S+P R N+ P+L + +++ F G ++ +G+ H SNG
Sbjct: 115 KSWWQLSNSEESSPFRETNYEPQLFLGFATDYR-FAG--WTLRDVEMGYNHDSNGRS--- 168

Query: 196 CYQPFNKEGNPENQFPGQPVIVKDYNGQKDVRWGGCRSVSAGQRPVFRLVWEKGGLKIMV 255
P ++ N R RL+ E G + V
Sbjct: 169 --DPTSRSWN---------------------------------RLYTRLMAENGNWLVEV 193

Query: 256 AYWPYVPYDQSNPNLIDYMGYGNAKVDYRRGRHHFELQLYDIFTQYWRYDRWHGAFRLGY 315
W V NP++ YMGY K+ Y G + QY ++ +G LG
Sbjct: 194 KPWYVVGNTDDNPDITKYMGYYQLKIGYHLGDAVLSAK-----GQY-NWNTGYGGAELGL 247

Query: 316 TYRINPFVGIYTQWFNGYGDGLYEYDVFSNRIGVGIRLN 354
+Y I V +YTQ ++GYG+ L +Y+ R+GVG+ LN
Sbjct: 248 SYPITKHVRLYTQVYSGYGESLIDYNFNQTRVGVGVMLN 286


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06475OMS28PORIN300.019 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 29.8 bits (66), Expect = 0.019
Identities = 13/37 (35%), Positives = 25/37 (67%)

Query: 309 EEDLLVSKKRLDKIYRLKQRVLGTLGGINPNFKKEIL 345
+E L+ S++ LD+ + Q+VL + G+NP+ K ++L
Sbjct: 188 KETLMASERALDETVQEAQKVLNMVNGLNPSNKDQVL 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06470VACCYTOTOXIN18780.0 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 1878 bits (4866), Expect = 0.0
Identities = 1053/1285 (81%), Positives = 1133/1285 (88%), Gaps = 32/1285 (2%)

Query: 1 MIIPAIVGGIATGAAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAGRGFNEFPNKEY 60
+IIPAIVGGIATGAAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAG+GFNEFPNKEY
Sbjct: 39 VIIPAIVGGIATGAAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAGKGFNEFPNKEY 98

Query: 61 DLYKSLLSSKIDGGWDWGNAARHYWVKGGQWNKLEVDMKDAVGTYKLSGLRNFTGGDLDV 120
DLYKSLLSSKIDGGWDWGNAARHYWVK GQWNKLEVDM++AVGTY LSGL NFTGGDLDV
Sbjct: 99 DLYKSLLSSKIDGGWDWGNAARHYWVKDGQWNKLEVDMQNAVGTYNLSGLINFTGGDLDV 158

Query: 121 NMQKATLRLGQFNGNSFTSFKDSADRTTRVDFNAKNISIDNFLEINNRVGSGAGRKASST 180
NMQKATLRLGQFNGNSFTS+KDSADRTTRVDFNAKNI IDNFLEINNRVGSGAGRKASST
Sbjct: 159 NMQKATLRLGQFNGNSFTSYKDSADRTTRVDFNAKNILIDNFLEINNRVGSGAGRKASST 218

Query: 181 VLTLQASEGITSGKNAEISLYDGATLNLASNSVKLMGNVWMGRLQYVGAYLAPSYSTINT 240
VLTLQASEGITS +NAEISLYDGATLNLASNSVKLMGNVWMGRLQYVGAYLAPSYSTINT
Sbjct: 219 VLTLQASEGITSRENAEISLYDGATLNLASNSVKLMGNVWMGRLQYVGAYLAPSYSTINT 278

Query: 241 SKVTGEVNFNHLTVGDRNAAQAGIIASKKTYIGTLDLWQSAGLNIIAPPEGGYKDKPNNT 300
SKVTGEVNFNHLTVGD NAAQAGIIAS KT+IGTLDLWQSAGLNIIAPPEGGYKDKPN+
Sbjct: 279 SKVTGEVNFNHLTVGDHNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPNDK 338

Query: 301 NSQSGAKNDKNESAKNDKQDSNTQVINPPNSGQKTEIQPTQVIDGPFAGAKDTVVNINRI 360
S + N KN+ ++ + +SNTQVINPPNS QKTEIQPTQVIDGPFAG K+TVVNINRI
Sbjct: 339 PSNTTQNNAKNDKQESSQNNSNTQVINPPNSAQKTEIQPTQVIDGPFAGGKNTVVNINRI 398

Query: 361 NTNADGTIKIGGYTASLTTNAANLNIGKGGINLSNQASGRSLLVENLTGNITVDGALMVN 420
NTNADGTI++GG+ ASLTTNAA+L+IGKGGINLSNQASGRSLLVENLTGNITVDG L VN
Sbjct: 399 NTNADGTIRVGGFKASLTTNAAHLHIGKGGINLSNQASGRSLLVENLTGNITVDGPLRVN 458

Query: 421 NQVGGYALAGSSANFEFKAGVDTKNGTATFNNDIHLGKAVNLRVDAHTAYFNGNIYLGKS 480
NQVGGYALAGSSANFEFKAG DTKNGTATFNNDI LG+ VNL+VDAHTA
Sbjct: 459 NQVGGYALAGSSANFEFKAGTDTKNGTATFNNDISLGRFVNLKVDAHTA----------- 507

Query: 481 TNLKVNGHSAHFKNIDASKSDNGLNTSTLDLSGVTDKVNINKLTTAATNVSIKNFDIKEL 540
+FK ID + G NT LD SGVT+KVNINKL TA+TNV++KNF+I EL
Sbjct: 508 ----------NFKGIDTG--NGGFNT--LDFSGVTNKVNINKLITASTNVAVKNFNINEL 553

Query: 541 VVTTRVQSFGQYTIFGENIGDQSRIGVVSLQTGYSPAYSGGVTFKSGKKLVIDEIYHAPW 600
VV T S G+YT F E+IG QSRI V L+TG YSGGV FK G+KLVI++ Y+APW
Sbjct: 554 VVKTNGVSVGEYTHFSEDIGSQSRINTVRLETGTRSIYSGGVKFKGGEKLVINDFYYAPW 613

Query: 601 NYFDARNVTDVEINKKILFGAPGNIAGKTGLMFNNLTLNSNASMDYGKDLDLTIQGHFTN 660
NYFDARN+ +VEI K+ FG G+ G LMFNNLTL NA MDY + +LTIQG F N
Sbjct: 614 NYFDARNIKNVEITNKLAFGPQGSPWGTAKLMFNNLTLGQNAVMDYSQFSNLTIQGDFVN 673

Query: 661 NQGVMNLFVQDGRVATLNAGHQASMMFNNMIDNTTGFYKPLIKVNNAQNLTKNKEHVLVK 720
NQG +N V+ G+VATLN G+ A+M F+N +D+ TGFY+PL+K+N+AQ+L KNKEHVL+K
Sbjct: 674 NQGTINYLVRGGQVATLNVGNAAAMFFSNNVDSATGFYQPLMKINSAQDLIKNKEHVLLK 733

Query: 721 ARNIDYNLVGVQGASYDNISASNTNLQEQFKERLALYNNNNRMDICVVRKDNLNDIKACG 780
A+ I Y G A D+I+ N NL EQFKERLALYNNNNRMDICVVR N +DIKACG
Sbjct: 734 AKIIGY---GNVSAGTDSIA--NVNLIEQFKERLALYNNNNRMDICVVR--NTDDIKACG 786

Query: 781 MAIGNQAMVNNPENYKYLEGKAWKNTGINKTANNTTIAVNLGNNSTPTSSESNTTNLPTN 840
AIGNQ+MVNNPENYKYLEGKAWKN GI+KTAN + I+V+ NSTPT + NTTNLPTN
Sbjct: 787 TAIGNQSMVNNPENYKYLEGKAWKNIGISKTANGSKISVHYLGNSTPTENGGNTTNLPTN 846

Query: 841 TTNNARFARYALIKNAPFAQTSATPNLVAINKHDFGTIESVFELANRSDDIDTLYAHSGA 900
TTN RFA YALIKNAPFA+ SATPNLVAIN+HDFGTIESVFELANRS+DIDTLYA+SGA
Sbjct: 847 TTNKVRFASYALIKNAPFARYSATPNLVAINQHDFGTIESVFELANRSNDIDTLYANSGA 906

Query: 901 QGRDLLQTLLIDSHNAGYARTMIDATSANEITKQLNTATDALNNIASLEHKTSGLQTLSL 960
QGRDLLQTLLIDSH+AGYARTMIDATSANEITKQLNTAT LNNIASLEHKTSGLQTLSL
Sbjct: 907 QGRDLLQTLLIDSHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLSL 966

Query: 961 SNAMILNSRLVNLSRRHTNNIDSFAQRLQALKDQKFASLESAAEVLYQFAPKYEKPTNVW 1020
SNAMILNSRLVNLSRRHTN+IDSFA+RLQALKDQ+FASLESAAEVLYQFAPKYEKPTNVW
Sbjct: 967 SNAMILNSRLVNLSRRHTNHIDSFAKRLQALKDQRFASLESAAEVLYQFAPKYEKPTNVW 1026

Query: 1021 ANAIGGASLNSGGNTSLYGTSAGVDAYLNEKVEAIVGGFGSYGYSSFNNQANSLNSGANN 1080
ANAIGG SLNSGGN SLYGTSAGVDAYLN +VEAIVGGFGSYGYSSF+NQANSLNSGANN
Sbjct: 1027 ANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGANN 1086

Query: 1081 ANFGVYSRIFANQHEFDFEAQGAVGSDQSSLNFKSALLRDLNQSYNYLAYGAATRASYGY 1140
NFGVYSRIFANQHEFDFEAQGA+GSDQSSLNFKSALLRDLNQSYNYLAY AATRASYGY
Sbjct: 1087 TNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASYGY 1146

Query: 1141 DFVFLRNALVLKPSVGVSYNHLGSTNFESNSTNKTALKNGASSQHLFNASANVEARYYYG 1200
DF F RNALVLKPSVGVSYNHLGSTNF+SNS K ALKNGASSQHLFNASANVEARYYYG
Sbjct: 1147 DFAFFRNALVLKPSVGVSYNHLGSTNFKSNSNQKVALKNGASSQHLFNASANVEARYYYG 1206

Query: 1201 DTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNTARNPLNTHARVMMGGELQLAKEVFLNL 1260
DTSYFYMNAGVLQEFANFGSSNAVSLNTFKVN RNPLNTHARVMMGGEL+LAKEVFLNL
Sbjct: 1207 DTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNATRNPLNTHARVMMGGELKLAKEVFLNL 1266

Query: 1261 GFIYLHNLISNAGHFASNLGMRYSF 1285
GF+YLHNLISN GHFASNLGMRYSF
Sbjct: 1267 GFVYLHNLISNIGHFASNLGMRYSF 1291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06440SECA290.005 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 28.7 bits (64), Expect = 0.005
Identities = 13/69 (18%), Positives = 33/69 (47%), Gaps = 12/69 (17%)

Query: 4 SPTKKDYTRYSEKQLFNLINQLEQKIKRMQNDRASFKEKMAKELEKRDQNFKDKIDALNK 63
S + R +++ N+IN +E +++++ ++ EL+ + F+ +++
Sbjct: 12 SRNDRTLRRM--RKVVNIINAMEPEMEKLSDE----------ELKGKTAEFRARLEKGEV 59

Query: 64 LLQKISQAF 72
L I +AF
Sbjct: 60 LENLIPEAF 68


10K747_06110K747_11745Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_06110315-0.006280glycerol-3-phosphate dehydrogenase
K747_06105517-1.723072hypothetical protein
K747_06100516-1.303005hypothetical protein
K747_11730415-1.057183hypothetical protein
K747_11735413-1.430116hypothetical protein
K747_11740614-2.837594hypothetical protein
K747_11745514-2.720585hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11740SSPAKPROTEIN270.022 Invasion protein B family signature.
		>SSPAKPROTEIN#Invasion protein B family signature.

Length = 133

Score = 27.2 bits (60), Expect = 0.022
Identities = 15/64 (23%), Positives = 31/64 (48%), Gaps = 8/64 (12%)

Query: 3 DFLKEMNKDCEDALNDQVKSFKKDFRKELYKEVLPVLREIINDDDLIDEVAFKKSVHAFT 62
+ L M + ++N+ V+ + D + L LR +I DD + D + F + +H F
Sbjct: 72 NILNLMLMNFSYSINELVELHRSD--EYL------QLRVVIKDDYVHDGIVFAEILHEFY 123

Query: 63 DKIK 66
+++
Sbjct: 124 QRME 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11745TYPE3OMGPROT330.003 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 32.6 bits (74), Expect = 0.003
Identities = 23/80 (28%), Positives = 37/80 (46%), Gaps = 13/80 (16%)

Query: 103 LFDR-VSNKEGIQRVYFVTSKIDTEIRNSNMALKSGHDLTTVLSNVKEV--LSSHLNKIM 159
LF R R++ + +I E ++AL +G DL T + V E+ S+ LNK++
Sbjct: 487 LFRRKSELTRRTVRLFIIEPRIIDEGIAHHLALGNGQDLRTGILTVDEISNQSTTLNKLL 546

Query: 160 EGL----------IQKWPNQ 169
G +QKW +Q
Sbjct: 547 GGSQCQPLNKAQEVQKWLSQ 566


11K747_05995K747_05960Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_059954151.388332cell division protein FtsA
K747_059904190.221143cell division protein FtsZ
K747_11770320-3.985803hypothetical protein
K747_05980114-4.565003hypothetical protein
K747_05975-113-4.302037mechanosensitive ion channel protein
K747_05970013-4.756792hypothetical protein
K747_05965014-4.725766hypothetical protein
K747_11775114-4.058580hypothetical protein
K747_05960214-3.428664hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_05995SHAPEPROTEIN424e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 41.7 bits (98), Expect = 4e-06
Identities = 39/181 (21%), Positives = 69/181 (38%), Gaps = 13/181 (7%)

Query: 211 AASIATLSNDERELGVACVDIGGETCNLTIYSGNSIRYNKYLPIGSHHLSMDLSSMLNTP 270
AA+I G VDIGG T + + S N + Y+ + IG + + +
Sbjct: 146 AAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRN 205

Query: 271 F------PYAEEVKIKYGDLSFESGEETPSQSVQIPTTGSDGNESHVVPLSRIQNIMRDR 324
+ AE +K + G S G+E V+ + + I +++
Sbjct: 206 YGSLIGEATAERIKHEIG--SAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEP 263

Query: 325 ALETFQIIHRSIQDSGLE---EHLGGGVVLTGGMALMKGIKELAKAHFTNYPVRLAA-PM 380
+ +++ E + G+VLTGG AL++ + L T PV +A P+
Sbjct: 264 LTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLL-MEETGIPVVVAEDPL 322

Query: 381 E 381

Sbjct: 323 T 323


12K747_05435K747_05405Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_05435216-1.561083hypothetical protein
K747_05430318-1.295030XRE family transcriptional regulator
K747_05425419-1.298693flgM protein
K747_05420318-1.378938hypothetical protein
K747_05415319-0.471839peptidyl-prolyl cis-trans isomerase
K747_054102180.855042hypothetical protein
K747_054052190.647250peptidoglycan-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_05405OMPADOMAIN1457e-45 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 145 bits (367), Expect = 7e-45
Identities = 45/168 (26%), Positives = 70/168 (41%), Gaps = 22/168 (13%)

Query: 22 NMDKETVAGDVSAKTVQSAPVSTETAQEKQEPKQEPAPVVEEKPAIESGTIIASIYFDFD 81
D ++ VS + Q P PAP V+ K T+ + + F+F+
Sbjct: 177 RPDNGMLSLGVSYRFGQGEAA----PVVAPAP--APAPEVQTK----HFTLKSDVLFNFN 226

Query: 82 KYEIKESDQETLDEIVQKAKE---NHMQVLLEGNTDEFGSSEYNQALGVKRTLSVKNALV 138
K +K Q LD++ + V++ G TD GS YNQ L +R SV + L+
Sbjct: 227 KATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLI 286

Query: 139 IKGVEKDMIKTISFGEIKPKCTQ-----KTR----ECYRENRRVDVKL 177
KG+ D I GE P K R +C +RRV++++
Sbjct: 287 SKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334


13K747_04080K747_03930Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_04080-2163.172344hypothetical protein
K747_04075-2162.766049hypothetical protein
K747_04070-1203.105203hypothetical protein
K747_04060-1182.493404hypothetical protein
K747_04055-1161.342732ribonuclease J
K747_04050015-1.64702516S rRNA methyltransferase
K747_04045017-2.015002hypothetical protein
K747_04040-117-2.101277hypothetical protein
K747_11905-216-3.081933hypothetical protein
K747_11910-215-2.604609hypothetical protein
K747_11915119-3.144358hypothetical protein
K747_04020224-2.604016hypothetical protein
K747_04015224-1.539491hypothetical protein
K747_04010224-0.486000hypothetical protein
K747_04005218-0.030761hypothetical protein
K747_04000217-1.206089hypothetical protein
K747_03995-115-0.243266hypothetical protein
K747_03990016-0.723534peptidylprolyl isomerase
K747_03985117-2.058217carbon storage regulator
K747_03980117-1.7917914-diphosphocytidyl-2C-methyl-D-erythritol
K747_03975015-1.006703single-stranded DNA-binding protein
K747_03970016-0.841312biopolymer transporter ExbB
K747_039651150.213603biopolymer transporter ExbD
K747_039552160.441849ribonuclease P
K747_039501140.558619hypothetical protein
K747_039452130.459281insertase
K747_039402120.320587spoIIIJ-associated protein
K747_039303111.232113membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_0394560KDINNERMP428e-147 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 428 bits (1103), Expect = e-147
Identities = 168/570 (29%), Positives = 281/570 (49%), Gaps = 59/570 (10%)

Query: 10 RLILAIALSFLFIAIYSYFFQKPNKT--TTPTTKQETTNNHTATNSNTPNAFSATQTIPQ 67
R +L IAL F+ I+ + Q N TT+ TT +A + P + +
Sbjct: 5 RNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKLISVK 64

Query: 68 ENLLSAISFEHARIEIDSLGR--IKQVYLKDKKYLTPKQKGFLEHVSHLFNPKANPQTPL 125
++L + I++ G + + K L Q L S F +A
Sbjct: 65 TDVL--------DLTINTRGGDVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTG 116

Query: 126 KELPLLAADKLKPLEVRFLDPTLNNKAFNTPYSASKTTLGPNEQLV--LTQDLGALTIIK 183
++ P A+ +PL +N A G NE V D T K
Sbjct: 117 RDGPDNPANGPRPL-------------YNVEKDAYVLAEGQNELQVPMTYTDAAGNTFTK 163

Query: 184 TLTFYDDLHYDLQIAFKSPN--------NIIPSYVITNGYKPVADLDS-----YTFSGVL 230
T Y + + + N + + P D S +TF G
Sbjct: 164 TFVLKRG-DYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTFRGAA 222

Query: 231 LENNDKKIEKIE---DKDAKEIKRFSNTLFLSSVDRYFTTLLFTKDPQGFEALIDSEVGT 287
D+K EK + D + + S +++ + +YF T + G + +G
Sbjct: 223 YSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHN-DGTNNFYTANLG- 280

Query: 288 KNPLGFISLKNEA-----------NLHGYIGPKDYRSLKAISPMLTDVIEYGLITFFAKG 336
N + I K++ N ++GP+ + A++P L ++YG + F ++
Sbjct: 281 -NGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLWFISQP 339

Query: 337 VFVLLDYLYQFVGNWGWAIILLTIIVRLILYPLSYKGMVSMQKLKEIAPKMKELQEKYKG 396
+F LL +++ FVGNWG++II++T IVR I+YPL+ SM K++ + PK++ ++E+
Sbjct: 340 LFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAMRERLGD 399

Query: 397 EPQKLQAHMMQLYKKHGANPLGGCLPLILQIPVFFAIYRVLYNAVELKSSEWILWIHDLS 456
+ Q++ MM LYK NPLGGC PL++Q+P+F A+Y +L +VEL+ + + LWIHDLS
Sbjct: 400 DKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAPFALWIHDLS 459

Query: 457 IMDPYFILPLLMGASMYWHQSVTPSSVTDPMQAKIFKFLPLLFTIFLITFPAGLVLYWTT 516
DPY+ILP+LMG +M++ Q ++P++VTDPMQ KI F+P++FT+F + FP+GLVLY+
Sbjct: 460 AQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYYIV 519

Query: 517 NNILSVLQQLIINKVLENKKRAHAQNKKES 546
+N+++++QQ +I + LE K+ H++ KK+S
Sbjct: 520 SNLVTIIQQQLIYRGLE-KRGLHSREKKKS 548


14K747_03855K747_03690Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_03855290.003101ABC transporter
K747_03850210-0.783475membrane protein
K747_03840211-0.695935membrane protein
K747_03835213-0.958427DNA polymerase I
K747_11945117-0.474565type II restriction endonuclease subunit R
K747_119501180.199408type I restriction endonuclease subunit S
K747_03825320-0.241931hypothetical protein
K747_038204141.017984amidophosphoribosyltransferase
K747_038153130.893432thymidylate kinase
K747_038101131.091151phosphopantetheine adenylyltransferase
K747_038052130.9538783-octaprenyl-4-hydroxybenzoate carboxy-lyase
K747_038003130.372426peptidase M73
K747_037952130.659959DNA helicase II
K747_037851140.412936seryl-tRNA synthetase
K747_037801140.361668nitrilase
K747_119552140.752012exodeoxyribonuclease VII small subunit
K747_037651120.884652membrane protein
K747_037601121.652055X-Pro dipeptidase
K747_037551111.554427antibiotic ABC transporter permease
K747_037502121.403773ABC transporter
K747_037453111.102532membrane protein
K747_037402110.287950lipase
K747_03730110-0.447198phosphate permease
K747_03725112-3.919314nitrogen-fixing protein NifU
K747_03720011-4.791403histidine kinase
K747_03710113-5.538414transaldolase
K747_03700116-6.776302peptidyl-tRNA hydrolase
K747_03695214-4.972141permease
K747_03690418-5.380541adenine methyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_03810LPSBIOSNTHSS2211e-77 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 221 bits (566), Expect = 1e-77
Identities = 65/148 (43%), Positives = 93/148 (62%)

Query: 4 IGIYPGTFDPVTNGHIDIIHRSSELFGKLIVAVAHSSAKNPMFSLKERLEMIQLATKSFK 63
IYPG+FDP+T GH+DII R LF ++ VAV + K PMFS++ERLE I A
Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61

Query: 64 NVECVAFEGLLANLAKEYHCKVLVRGLRVVSDFEYELQMGYANKSLNHELETLYFMPTLQ 123
N + +FEGL N A++ ++RGLRV+SDFE ELQM NK+L +LET++ + +
Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121

Query: 124 NAFISSSIVRSIILHKGDASHLVPKEIH 151
+F+SSS+V+ + G+ H VP +
Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHVA 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_03755ABC2TRNSPORT352e-04 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 35.3 bits (81), Expect = 2e-04
Identities = 29/118 (24%), Positives = 50/118 (42%), Gaps = 1/118 (0%)

Query: 246 SFYGIERHTSALMVFLNSSIFMLATLSLGLFLGAWIKNEAHTTQIVLISSLPLIFMMGFV 305
+ G + S L ++ LA SLG+ + A + + + P++F+ G V
Sbjct: 137 AALGYTQWLSLLYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAV 196

Query: 306 WPFESLPSYLQAFVQIVPAYHAISLLGRLNQMHAEFIDVSIHFYALIAIFIASFIASV 363
+P + LP Q + +P H+I L+ R + +DV H AL + F S
Sbjct: 197 FPVDQLPIVFQTAARFLPLSHSIDLI-RPIMLGHPVVDVCQHVGALCIYIVIPFFLST 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_03745RTXTOXIND461e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 46.4 bits (110), Expect = 1e-07
Identities = 27/200 (13%), Positives = 63/200 (31%), Gaps = 29/200 (14%)

Query: 8 KNKAILTGGGALLLGLIVLFYLAYRP--KAEVLQGFL--EAREYSVSSKVPGRIEKVFVK 63
+ L + +I G L R + ++++ VK
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 64 KGDHIKKGDLVFSISSPELEAKLAQAEAGHKAAK-------ALSDEVKRGSRDETINSAR 116
+G+ ++KGD++ +++ EA + ++ A+ LS ++ E
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 117 DIWQAAKSQATLAKETYKRVQDLYDNGVASLQKRDEAYAAYESTKYNESAAYQKYKMALG 176
+Q ++E R+ L E ++ +++ KY + K +
Sbjct: 174 PYFQNV------SEEEVLRLTSLI----------KEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 177 GASSESKIAAKAKESEALGQ 196
++I S
Sbjct: 218 TV--LARINRYENLSRVEKS 235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_03730ALARACEMASE290.041 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 29.4 bits (66), Expect = 0.041
Identities = 12/59 (20%), Positives = 26/59 (44%), Gaps = 2/59 (3%)

Query: 56 IGGYMAMNIGANDVSNNVGPAVGSKAISMGGAILIAGICEMLGAIIAGGEVVSTIKGRI 114
G ++M++ A D++ +G+ G I I + G + G E++ + R+
Sbjct: 295 TVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTV--GYELMCALALRV 351


15K747_03640K747_03485Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_036403132.335413saccharopine dehydrogenase
K747_119751131.4308004Fe-4S ferredoxin
K747_03625-1111.341280glycerol-3-phosphate acyltransferase
K747_03620-310-0.952563dihydroneopterin aldolase
K747_03615-210-0.937759iron-regulated outer membrane protein FrpB4
K747_03610-210-1.376200TonB-dependent receptor
K747_03605-110-4.266073selenocysteine synthase
K747_03600-110-4.125092transcription elongation factor NusA
K747_03595012-4.763626type IIS restriction-modification protein
K747_03590113-4.121242hypothetical protein
K747_11980113-3.766096hypothetical protein
K747_11985011-2.798474type II restriction endonuclease
K747_03570012-1.998990ATP-dependent DNA helicase RecG
K747_03565115-1.876310hypothetical protein
K747_03560-214-0.812875membrane protein
K747_03555-115-0.693853exodeoxyribonuclease III
K747_03550116-0.531217*competence protein
K747_03540422-0.678934purine nucleoside phosphorylase
K747_03535218-2.246970hypothetical protein
K747_03530214-1.824065glucosamine--fructose-6-phosphate
K747_03525014-3.016174FAD-dependent thymidylate synthase
K747_03520-1150.244806hypothetical protein
K747_03515-1150.027189hypothetical protein
K747_03510-2140.113148type I restriction endonuclease subunit S
K747_03505-2121.719948type I restriction endonuclease subunit M
K747_034902123.481087metal-dependent hydrolase
K747_034852133.384556ligand-gated channel
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_03505TYPE4SSCAGA320.014 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 31.6 bits (71), Expect = 0.014
Identities = 46/172 (26%), Positives = 79/172 (45%), Gaps = 20/172 (11%)

Query: 641 IPKNLIKQRFFEKE-AKELEELENALNEKEADFEEFIEEHSSEEGLFYELKINESVLKKE 699
+ K+L K+ EKE K+LE N+ EA + +S++ + L IN+ +
Sbjct: 614 LEKSLRKREHLEKEVEKKLESKSGNKNKMEAK-----AQANSQKDEIFAL-INKEANRDA 667

Query: 700 LKNATDLEDKKILKTALELLEAKNKALKMKNKAYEELE-------LKAFHQYKNLKLGEI 752
A K I + + LE NK LK +K+++E + KA K LK G +
Sbjct: 668 RAIAYAQNLKGIKRELSDKLENVNKNLKDFDKSFDEFKNGKNKDFSKAEETLKALK-GSV 726

Query: 753 KDLIIQDKWLKSLKNALENKILKRINAFTSALNEIISNYSNSLLELDKEVKE 804
KDL I +W+ ++N + +N F + N+ S + + +L+ VK+
Sbjct: 727 KDLGINPEWISKVEN-----LNAALNEFKNGKNKDFSKVTQAKSDLENSVKD 773


16K747_08360K747_08395N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_08360-1131.332154flagellar hook-basal body protein FliE
K747_08365-1131.357959flagellar basal body rod protein FlgC
K747_08370-1120.172415flagellar basal body rod protein FlgB
K747_08380-1140.061704iron ABC transporter substrate-binding protein
K747_083850140.319550peroxidase
K747_08390013-0.382567ABC transporter substrate-binding protein
K747_08395014-0.529430penicillin-binding protein 2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_08360FLGHOOKFLIE776e-22 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 77.0 bits (189), Expect = 6e-22
Identities = 19/77 (24%), Positives = 40/77 (51%), Gaps = 1/77 (1%)

Query: 34 EQKGGEFSKLLKQSINELNNTQEQSDKALADMATGQIK-DLHQAAIAIGKAETSMKLMLE 92
Q F+ L +++ +++TQ + G+ L+ + KA SM++ ++
Sbjct: 27 PQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQ 86

Query: 93 VRNKAISAYKELLRTQI 109
VRNK ++AY+E++ Q+
Sbjct: 87 VRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_08365FLGHOOKAP1280.012 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 28.4 bits (63), Expect = 0.012
Identities = 10/38 (26%), Positives = 15/38 (39%)

Query: 125 NVNAVVEMADLVEATRAYQANVAAFQSAKNMAQNAIGM 162
VN E +L + Y AN Q+A + I +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_08380FERRIBNDNGPP352e-04 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 35.3 bits (81), Expect = 2e-04
Identities = 30/184 (16%), Positives = 76/184 (41%), Gaps = 12/184 (6%)

Query: 106 NVELLKKLSPDLVVTFVGNPKAVEHAKKF--GILFLSFQEKTIAEVMEDID---AQAKAL 160
N+ELL ++ P +V G + E + G F K + A L
Sbjct: 88 NLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNL 147

Query: 161 EIDASKKLAKMQETLDFIKERLKDVKNKKGVELFHKAN----KISGHQALDSDILEKGGV 216
+ A LA+ ++ + +K R + + + L + + G +L +IL++ G+
Sbjct: 148 QSAAETHLAQYEDFIRSMKPRFVK-RGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGI 206

Query: 217 DN-FGLKYVKFGRADVSVEKIVK-ENPEIIFIWWVSPLTPEDVLNNPKFSTIKAIKNKQV 274
N + + +G VS++++ ++ +++ + + ++ P + + ++ +
Sbjct: 207 PNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRF 266

Query: 275 YKLP 278
++P
Sbjct: 267 QRVP 270


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_08395TYPE3IMPPROT290.030 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 29.4 bits (66), Expect = 0.030
Identities = 9/23 (39%), Positives = 12/23 (52%)

Query: 4 LRYKLLLFVFIGFWGLLVLNLFI 26
KL+LFV + W LL L +
Sbjct: 195 TPIKLVLFVALDGWTLLSKGLIL 217


17K747_09895K747_09915N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_09895-3141.072390DNA polymerase sliding clamp subunit
K747_09900-3120.848388histidine kinase
K747_09905-2132.081767hypothetical protein
K747_09910-2122.099835flagellar P-ring protein FlgI
K747_09915-3112.029185DEAD/DEAH box helicase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09895HELNAPAPROT1501e-49 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 150 bits (380), Expect = 1e-49
Identities = 39/140 (27%), Positives = 75/140 (53%), Gaps = 1/140 (0%)

Query: 5 EILKHLQADAIVLFMKVHNFHWNVKGTDFFNVHKATEEIYEGFADMFDDLAERIVQLGHH 64
L ++ +L+ K+H FHW VKG FF +H+ EE+Y+ A+ D +AER++ +G
Sbjct: 15 NSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIGGQ 74

Query: 65 PLVTLSEALKLTRVKEETKTSFHSKDIFKEILEDYKHLEKEFKELSNTAEKEGDKVTVTY 124
P+ T+ E + + + + + ++ + ++ DYK + E K + AE+ D T
Sbjct: 75 PVATVKEYTEHASITDGGNET-SASEMVQALVNDYKQISSESKFVIGLAEENQDNATADL 133

Query: 125 ADDQLAKLQKSIWMLQAHLA 144
+ +++K +WML ++L
Sbjct: 134 FVGLIEEVEKQVWMLSSYLG 153


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09900PF06580300.016 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.8 bits (67), Expect = 0.016
Identities = 10/71 (14%), Positives = 25/71 (35%), Gaps = 13/71 (18%)

Query: 281 IVLQNFLYNAIDAIEALEESEQ-GQVKIEAFIQNEFIVFTIIDNGKEVENKSALFEPFET 339
+++Q + N I + + Q G++ ++ N + + + G +
Sbjct: 258 MLVQTLVENGI--KHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK------- 308

Query: 340 TKLKGNGLGLA 350
+ G GL
Sbjct: 309 ---ESTGTGLQ 316


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09910FLGPRINGFLGI355e-124 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 355 bits (913), Expect = e-124
Identities = 121/345 (35%), Positives = 194/345 (56%), Gaps = 26/345 (7%)

Query: 19 AEKIGDIASVVGVRDNQLIGYGLVIGLNGTGDK-SGSKFTMQSISNMLESVNVKISADDI 77
+I DIAS+ RDNQLIGYGLV+GL GTGD S FT QS+ ML+++ +
Sbjct: 28 TSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQS 87

Query: 78 KSKNVAAVMITASLPPFARQGDKIDVQISSIGDAKSIQGGTLVMTPLNAVDGNIYALAQG 137
+KN+AAVM+TA+LPPFA G ++DV +SS+GDA S++GG L+MT L+ DG IYA+AQG
Sbjct: 88 NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQG 147

Query: 138 AITSGNSS-----------NLLSAHIINGATIEREVSYDLFHKNAMVLSLKNPNFKNAIQ 186
A+ S SA + NGA IERE+ +VL L+NP+F A++
Sbjct: 148 ALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPDFSTAVR 207

Query: 187 VQNTLNKV----FGNKVAIALDPKTIQITRPERFSMVEFLALVQEIPINYSTKNKIIVDE 242
V + +N +G+ +A D + I + +P + +A ++ + + T K++++E
Sbjct: 208 VADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPAKVVINE 267

Query: 243 KSGTIVSGVDIIVHPIVVTSQDITLKITKEPLDN--------SKNAQDLDNNMSLDTAHN 294
++GTIV G D+ + + V+ +T+++T+ P Q + M++
Sbjct: 268 RTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGSK 327

Query: 295 TLSSNGKNITIAGVVKALQKIGVSANGMVSILQALKKSGAISAEM 339
G ++ +V L IG+ A+G+++ILQ +K +GA+ AE+
Sbjct: 328 VAIVEGPDLR--TLVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_09915SECA300.028 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.028
Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 2/63 (3%)

Query: 261 IVFTRTKKEADELHQFLASKNYKSTALHGDMDQRDRRASIMAFKKNDADVLVATDVASRG 320
+V T + ++++ + L K L+ + A+I+A A V +AT++A RG
Sbjct: 453 LVGTISIEKSELVSNELTKAGIKHNVLNAKFHANE--AAIVAQAGYPAAVTIATNMAGRG 510

Query: 321 LDI 323
DI
Sbjct: 511 TDI 513


18K747_10430K747_10465N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_10430013-0.152723flagellar assembly protein FliH
K747_10445-113-1.213808phenylalanyl-tRNA synthetase subunit alpha
K747_10450-1120.369273hypothetical protein
K747_104550110.095817flagellar basal body rod protein FlgG
K747_10460011-0.190155alpha-ketoglutarate permease
K747_10465-112-0.140022cell division protein FtsK
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10430FLGFLIH373e-05 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 37.5 bits (86), Expect = 3e-05
Identities = 45/207 (21%), Positives = 91/207 (43%), Gaps = 14/207 (6%)

Query: 50 PLEKKAIENDLIDCLLKKTDELSSHLVKLQMQFEKAQEES-KALIENAKNDGYKIGFKEG 108
E I + + L L +LQMQ A E+ +A I + G+K G++EG
Sbjct: 19 QAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQ---AHEQGYQAGIAEGRQQGHKQGYQEG 75

Query: 109 EEKMRNELTHSVNEEKNQLLHAITALDEKMKKSQDHLMALE----KELSAIAIDIAKEVI 164
+ L + E K+Q + + + + Q L AL+ L +A++ A++VI
Sbjct: 76 ---LAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVI 132

Query: 165 LKEVEDNSQKVALALAEELLKNVLDATDIHLKVNPLDYPYLNERLQNASKI---KLESNE 221
+ ++ + + + L + L + L+V+P D +++ L + +L +
Sbjct: 133 GQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGATLSLHGWRLRGDP 192

Query: 222 AISKGGVMITSSNGSLDGNLMERFKTL 248
+ GG +++ G LD ++ R++ L
Sbjct: 193 TLHPGGCKVSADEGDLDASVATRWQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10455FLGHOOKAP1300.008 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 30.3 bits (68), Expect = 0.008
Identities = 9/40 (22%), Positives = 16/40 (40%)

Query: 3 NGYYAATGAMATQFNRLDLTSNNLANLNTNGFKRDDAITG 42
+ A + L+ SNN+++ N G+ R I
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMA 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10460TCRTETB393e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 39.1 bits (91), Expect = 3e-05
Identities = 57/315 (18%), Positives = 104/315 (33%), Gaps = 67/315 (21%)

Query: 37 APYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKKGRKTSMVYSVILMA 96
P A +F T + +AF++ G+ +GKL D+ G K +++ +I+
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSI------GTAVYGKLSDQLGIKRLLLFGIIINC 90

Query: 97 LGSFLLALLPTKEIVGEWAFLFLLLARLLQGFSVGGE------YGVVATYLSELGKNGKK 150
GS + VG F L++AR +QG G VVA Y+ + +
Sbjct: 91 FGSVIGF-------VGHSFFSLLIMARFIQG--AGAAAFPALVMVVVARYIPKENRGKAF 141

Query: 151 GFYGSFQYVTLVGGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALFSLFLRNIM 210
G GS + +G + I I+ W YL + I + FL ++
Sbjct: 142 GLIGS---IVAMGEGVGPAIGGMIAHYIH---------WSYLLLIPMITIITVPFLMKLL 189

Query: 211 EETMDGQTTFKTTIKEKTQRGSLKELLNHKKALM-------IVFGLTMGGSLCFYTFTVY 263
+ + +K + K ++ + T +
Sbjct: 190 K-----------------KEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLS 232

Query: 264 LKIFLTNSSSFSPK-------ESSFIMLLALSYFIFLQPLCG---MLADKIKRTQMLMVF 313
IF+ + + ++ M+ L I + G M+ +K L
Sbjct: 233 FLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA 292

Query: 314 AIAGLIVTPVVFYGI 328
I +I+ P I
Sbjct: 293 EIGSVIIFPGTMSVI 307


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_10465IGASERPTASE330.008 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.7 bits (74), Expect = 0.008
Identities = 33/160 (20%), Positives = 53/160 (33%), Gaps = 30/160 (18%)

Query: 248 YPKRNRFNDLSNPTNPTLKE-----VKQETKER-------EPTPKPATLKPVMPASAPNT 295
KRN+ D +N T P + V +E P P PAT A N+
Sbjct: 985 VEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENS 1044

Query: 296 DNDNKTE--NHKAPNHPIKEENPQEENIKENIKENIKEN----------------IKENI 337
++KT N + + + K N+K N + N KE
Sbjct: 1045 KQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETA 1104

Query: 338 KERIKERIKEEENEAQNAPSFSPITPTSAKKPVMVKELSE 377
+E+ K E + Q P + ++ V+ +E
Sbjct: 1105 TVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAE 1144


19K747_11180K747_11220N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_11180-29-0.853111type I restriction endonuclease subunit S
K747_11185-2110.525742hypothetical protein
K747_11190-1111.829747restriction endonuclease subunit R
K747_111950123.060835hydroxyethylthiazole kinase
K747_11200-1133.456302phosphomethylpyrimidine kinase
K747_112150142.662189phosphopantothenate synthase
K747_112200153.055451N-acetyl glucosamine/N-acetyl galactosamine
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11180BONTOXILYSIN290.014 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 29.1 bits (65), Expect = 0.014
Identities = 19/86 (22%), Positives = 33/86 (38%), Gaps = 7/86 (8%)

Query: 110 LNSFCFGFRFFDENLFNPSFLKHFLRDYNFRKNISKVANGVTRFNVSKQLLSKITIPIPP 169
LN FC F + F+ + LKHF R + + + +N++ + +I +P
Sbjct: 336 LNYFCQSFNSIIPDRFS-NALKHFYRKQYYTMDYTD------NYNINGFVNGQINTKLPL 388

Query: 170 LEIQQEIVKILDAFTELNTELNTELK 195
I+ + L E N L
Sbjct: 389 SNKNTNIISKPEKVVNLVNENNISLM 414


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11190INFPOTNTIATR300.026 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 30.0 bits (67), Expect = 0.026
Identities = 24/70 (34%), Positives = 36/70 (51%), Gaps = 8/70 (11%)

Query: 633 QEVHAYFQDYIHQKREEEFQKLIEENRLNEEKAYLFMQHAFKGGEISF-SGTEFPKII-- 689
++V + FQ + KR EF K EEN+ + A+L + K G + SG ++ KII
Sbjct: 79 KDVLSKFQKDLMAKRSAEFNKKAEENKAKGD-AFL-SANKSKPGIVVLPSGLQY-KIIDA 135

Query: 690 --GEKPSRFD 697
G KP + D
Sbjct: 136 GTGAKPGKSD 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11200PF06057280.038 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 27.9 bits (62), Expect = 0.038
Identities = 12/55 (21%), Positives = 21/55 (38%), Gaps = 7/55 (12%)

Query: 138 TPNLPEVFALTGVQV-----RDDKSASKAMGILRDLGVKNAVIKGGHTEHFQGEF 187
LPEV T V + ++D + ++ V + GGH F ++
Sbjct: 177 YLTLPEVNKQTTVPMLCLYGKEDDAPLHLCPEVKQPNVTVMELSGGH--SFDDDY 229


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11220NUCEPIMERASE701e-15 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 70.2 bits (172), Expect = 1e-15
Identities = 44/238 (18%), Positives = 96/238 (40%), Gaps = 34/238 (14%)

Query: 6 TILITGGTGSFGKRFARKVLDTTNAKKIIV------YSRDELKQSEMAMEFNDPRMRFFI 59
L+TG G G ++++L+ +++ Y LKQ+ + + P +F
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLE--AGHQVVGIDNLNDYYDVSLKQARLEL-LAQPGFQFHK 58

Query: 60 GDVRDLERLN--YALEGVGICIHAAAIKHVPIAEYNPLECIKTNIMGASNVINACLKNEV 117
D+ D E + +A + V + NP +N+ G N++ C N++
Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118

Query: 118 SQVIALST---------------DKAANPINLYGATKLCSDKLFVSANNFKGSSQTQFSV 162
++ S+ D +P++LY ATK ++ + + ++ G T
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGL-- 176

Query: 163 VRYGNVVGSRG----SVVPFFKKLVQNKASEIPITDIRMTRFWITLDEGVSFVLKSLK 216
R+ V G G ++ F K +++ K+ ++ +M R + +D+ +++
Sbjct: 177 -RFFTVYGPWGRPDMALFKFTKAMLEGKSIDV-YNYGKMKRDFTYIDDIAEAIIRLQD 232


20K747_11615K747_07090N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_11615-312-0.619268lipopolysaccharide biosynthesis protein
K747_07165010-0.119018adenylate kinase
K747_0716019-0.101258aspartyl-tRNA synthetase
K747_07155190.459078chemotaxis protein CheV
K747_07145190.595077hypothetical protein
K747_11620190.518459hypothetical protein
K747_11625090.584931toxin
K747_07125-2121.273315membrane protein
K747_07120-3120.881887acriflavin resistance protein
K747_07115-3130.535262membrane protein
K747_07110-3120.126248membrane protein
K747_07105-214-0.825037uroporphyrinogen decarboxylase
K747_07100-115-1.849369hypothetical protein
K747_07095115-1.5125863-methyladenine DNA glycosylase
K747_07090014-1.116577flagellin
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11615FLGHOOKFLIE320.002 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 31.6 bits (71), Expect = 0.002
Identities = 19/90 (21%), Positives = 37/90 (41%), Gaps = 10/90 (11%)

Query: 161 EAEAPIENHEVTPPPNSTQDTQQ-----DFINETQQNPKEPSDPCKIAPQKISFNQVVFK 215
+A A + + P + Q D I++TQ + ++ + ++ N V+
Sbjct: 14 QATAMSARAQESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTD 73

Query: 216 KIKRKLNHFIGNILARTEVYKKLVAKYDEL 245
K ++ +G +V KLVA Y E+
Sbjct: 74 MQKASVSMQMG-----IQVRNKLVAAYQEV 98


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07165MALTOSEBP290.017 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 28.5 bits (63), Expect = 0.017
Identities = 20/52 (38%), Positives = 30/52 (57%), Gaps = 5/52 (9%)

Query: 60 GELVPLEIVVETILSAIKSSDKGIILIDGYPRSVEQMQALDKELSAQNEVVL 111
G+L+ I VE LS I + D L+ P++ E++ ALDKEL A+ + L
Sbjct: 127 GKLIAYPIAVEA-LSLIYNKD----LLPNPPKTWEEIPALDKELKAKGKSAL 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07155HTHFIS543e-10 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 54.1 bits (130), Expect = 3e-10
Identities = 24/110 (21%), Positives = 44/110 (40%), Gaps = 6/110 (5%)

Query: 194 ILIAEDSLSALKTLEKIVQTLELRYLAFPNGKELLDYLYEKEHYQQVGVVITDLEMPVIS 253
IL+A+D + L + + N L ++ +V+TD+ MP +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAA----GDGDLVVTDVVMPDEN 61

Query: 254 GFEVLKIIKADSRTEHLPVIINSSMSSDSNRQLAQSLEADGFVVKSNILE 303
F++L IK LPV++ S+ ++ A A ++ K L
Sbjct: 62 AFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07145LCRVANTIGEN300.001 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 30.0 bits (67), Expect = 0.001
Identities = 15/33 (45%), Positives = 20/33 (60%)

Query: 16 KRKRLLTELAELEAEIKVSSERKSSFNISLSPS 48
R +L ELAEL AE+K+ S ++ N LS S
Sbjct: 149 ARSKLREELAELTAELKIYSVIQAEINKHLSSS 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11625VACCYTOTOXIN2703e-75 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 270 bits (692), Expect = 3e-75
Identities = 102/394 (25%), Positives = 182/394 (46%), Gaps = 14/394 (3%)

Query: 2809 NTVNWLNALFVAKGGNPLFAPYYLQDNSTKHIVTLMKDITSALGMLSKSNLKNNSTDVLQ 2868
+ L L + + +A + S I + T+ L ++ K + L
Sbjct: 907 QGRDLLQTLLI-DSHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLS 965

Query: 2869 LNTYTQQMGRLAKLSSFASFDSTDFSERLSSLKNQRFADAIPNAMDVILKYSQRDKLKNN 2928
L+ RL LS + F++RL +LK+QRFA + +A +V+ +++ + + N
Sbjct: 966 LSNAMILNSRLVNLSRRHTNHIDSFAKRLQALKDQRFAS-LESAAEVLYQFAPKYEKPTN 1024

Query: 2929 LWATGVGGVSFVENGTGTLYGINVGYDRFIKG---VIVGGYAAYGYSGFYER--ITSSKS 2983
+WA +GG S G +LYG + G D ++ G IVGG+ +YGYS F + +S +
Sbjct: 1025 VWANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGA 1084

Query: 2984 DNVDVGLYARAFIKKSELTFSVNETWGANKTQISSNDTLLSMINQSYQYSTWTTNARVNY 3043
+N + G+Y+R F + E F G++++ ++ LL +NQSY Y ++ R +Y
Sbjct: 1085 NNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASY 1144

Query: 3044 GYDFMFKNKSVIVKPQIGLRYYYIGMTGLEGVMNNALYNQFKANADPSKKSVLMIDFAFE 3103
GYDF F ++++KP +G+ Y ++G T + + S + + E
Sbjct: 1145 GYDFAFFRNALVLKPSVGVSYNHLGSTNFKS----NSNQKVALKNGASSQHLFNASANVE 1200

Query: 3104 NRHYFNKNSYFYTIGGIGRDLLVRSMGDKLVRFIGDNILSYRKGELYNTFVNITTGGEIR 3163
R+Y+ SYFY G+ ++ + V + + R NT + GGE++
Sbjct: 1201 ARYYYGDTSYFYMNAGVLQEFANFGSSNA-VSLNTFKVNATRNP--LNTHARVMMGGELK 1257

Query: 3164 LFKSFYVNAGVGARFGLDYKMINITGNIGMRLAF 3197
L K ++N G L + + N+GMR +F
Sbjct: 1258 LAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF 1291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07120ACRIFLAVINRP8980.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 898 bits (2321), Expect = 0.0
Identities = 284/1038 (27%), Positives = 516/1038 (49%), Gaps = 40/1038 (3%)

Query: 1 MYKTAINRPITTLMFALAIVFFGTMGFKKLSVALFPKIDLPTVVVTTTYPGASAEIIESK 60
M I RPI + A+ ++ G + +L VA +P I P V V+ YPGA A+ ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTDKIEEAVMGIDGIKKVTSTSSKNVSIVV-IEFELEKPNEEALNDVVNKISSVR-FDDS 118
VT IE+ + GID + ++STS S+ + + F+ + A V NK+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 119 NIKKPSINKFDTDSQAIISLFVSSSSVPAT--TLNDYTKNTIKPMLQKIDGVGGVQLNGF 176
+++ I+ + S ++ S + T ++DY + +K L +++GVG VQL G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 177 RERQIRIYADPTLMNKYNLTYADLFSTLKAENVEIDGGRIVNS------QRELAILVNAN 230
+ +RI+ D L+NKY LT D+ + LK +N +I G++ + Q +I+
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 231 SYSVADVEKIQV-----GNHVRLGDIAKIEIGLEEDNTFASFKDKPGVILEIQKIAGANE 285
+ + K+ + G+ VRL D+A++E+G E N A KP L I+ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 286 IEIVDRVYEALKHIQAISP-NYEIRPFLDTTGYIRTSIEDVKFDLVLGAILAVLVVFAFL 344
++ + L +Q P ++ DTT +++ SI +V L +L LV++ FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 345 RNGTITLVSAISIPISVMGTFALIQWMGFSLNMLTMVALTLAIGIIIDDAIVVIENIHK- 403
+N TL+ I++P+ ++GTFA++ G+S+N LTM + LAIG+++DDAIVV+EN+ +
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 404 KLEMGMNKRKASYEGVREIGFALVAISAMLLSVFVPIGNMKGIIGRFFQSFGITVALAIA 463
+E + ++A+ + + +I ALV I+ +L +VF+P+ G G ++ F IT+ A+A
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 464 LSYVVVVTIIPMVSSVVVNPRHS-------RFYVWSEPFFKALESRYTKLLQWVLNHKLI 516
LS +V + + P + + ++ P + F+ W F + YT + +L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 517 IFIAVVLVFVGSLFVASKLGMEFMLKEDRGRFLVWLKAKPGVSIDY----MTQKSKIFQK 572
+ L+ G + + +L F+ +ED+G FL ++ G + + + Q + + K
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 573 AIEKHAEVEFTTLQVGY-GTTQNPFKAKIFVQLKPLKERKKEHELGQFELMSTLRKELKS 631
+ + E FT + G QN FV LKP +ER E ++ + EL
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNA--GMAFVSLKPWEERNG-DENSAEAVIHRAKMELGK 656

Query: 632 MPEAKGLESINLSEVSLIGGGGDSSPFQTFVFSHSQEAVDKSVANLKKFLLESPELKGKI 691
+ + + N+ + G ++ F + + D + L + + +
Sbjct: 657 IRDGF-VIPFNMPAIV---ELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASL 712

Query: 692 EGYHTSTSESQPQLQLKILRQNANKYGVSAQTIGSVVSSAFSGTSQASVFKEDGKEYDMI 751
+ E Q +L++ ++ A GVS I +S+A G + + F + G+ +
Sbjct: 713 VSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGG-TYVNDFIDRGRVKKLY 771

Query: 752 IRVPDNKRVSVEDIKRLQVRNKYDKLMFLDALVEITETKSPSSISRYNRQRSVTVLAQPK 811
++ R+ ED+ +L VR+ +++ A + RYN S+ + +
Sbjct: 772 VQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAA 831

Query: 812 AGISLGEILTQVSKNTKEWLVEGANYRFTGEADNAKESNGEFLIALATAFVLIYMILAAL 871
G S G+ + + +N L G Y +TG + + S + +A +FV++++ LAAL
Sbjct: 832 PGTSSGDAMALM-ENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAAL 890

Query: 872 YESILEPFIIMVTMPLSFSGAFFALGLVHQPLSMFSMIGLILLIGMVGKNATLLIDVANE 931
YES P +M+ +PL G A L +Q ++ M+GL+ IG+ KNA L+++ A +
Sbjct: 891 YESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKD 950

Query: 932 -ERKKGLNIQEAILFAGKTRLRPILMTTIAMVCGMLPLALASGDGAAMKSPIGIAMSGGL 990
K+G + EA L A + RLRPILMT++A + G+LPLA+++G G+ ++ +GI + GG+
Sbjct: 951 LMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGM 1010

Query: 991 MISMVLSLLIVPVFYRLL 1008
+ + +L++ VPVF+ ++
Sbjct: 1011 VSATLLAIFFVPVFFVVI 1028


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07115RTXTOXIND511e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.0 bits (122), Expect = 1e-09
Identities = 22/69 (31%), Positives = 34/69 (49%)

Query: 40 STGIVDSIKVTEGSVVKKGDVLLLLYNQDKQAQSDSTEQQLIFAKKQYQRYSKIGGAVDK 99
IV I V EG V+KGDVLL L +A + T+ L+ A+ + RY + +++
Sbjct: 103 ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIEL 162

Query: 100 NTLESYEFN 108
N L +
Sbjct: 163 NKLPELKLP 171



Score = 31.3 bits (71), Expect = 0.003
Identities = 21/152 (13%), Positives = 48/152 (31%), Gaps = 25/152 (16%)

Query: 70 QAQSDSTEQQLIFAKKQYQR--YSKIGGAVDKNTLESYEFNYRRLESDYAYSIALLNKTI 127
+++ S +++ + ++ K+ D + + E ++
Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTTDN--IGLLTLELAKNEER-------QQASV 329

Query: 128 LRAPFDGVIASKNIQVGEGVSANNTVLLRLVSHARKLVIE--FDSKYINAVKVG------ 179
+RAP + + GV L+ +V L + +K I + VG
Sbjct: 330 IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIK 389

Query: 180 -DTYTYSIDGDSNQHEAKITKIYP--TVDENT 208
+ + Y+ G K+ I D+
Sbjct: 390 VEAFPYTRYGYL---VGKVKNINLDAIEDQRL 418


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07110RTXTOXIND290.047 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.0 bits (65), Expect = 0.047
Identities = 16/113 (14%), Positives = 41/113 (36%), Gaps = 16/113 (14%)

Query: 203 LARMIALQKKLEQIKTDIKRVTKLYDKGLTTIDDL-----QSLKAQGNLSEY--DILDMQ 255
LAR+ + K+ + + L K + + ++A L Y + ++
Sbjct: 220 LARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIE 279

Query: 256 FALEQNRLTLEYLTNLSVKNLKKTTIDVPNLQLRERQD-LVSLREQISALKYQ 307
+ + + +T K +D +LR+ D + L +++ + +
Sbjct: 280 SEILSAKEEYQLVTQ----LFKNEILD----KLRQTTDNIGLLTLELAKNEER 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07095PF05272300.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.006
Identities = 12/95 (12%), Positives = 25/95 (26%), Gaps = 20/95 (21%)

Query: 60 ILENDDETNLKKIAYADFSKLAECVRPSGFYNQKAKRLINLSENILKDFQSFENFKQEAT 119
L + +A+ + + VR + +KA E+
Sbjct: 458 ALRSAPALA-GCVAFDELREQPVAVRAFPW--RKAPGP-------------LEDADVLRL 501

Query: 120 REWLLDQKGIGKESADAILCYVCAREVMVVDKYSY 154
+++ G G+ SA + D
Sbjct: 502 ADYVETTYGTGEASAQTTEQAINV----AADMNRV 532


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_07090FLAGELLIN2431e-76 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 243 bits (622), Expect = 1e-76
Identities = 125/518 (24%), Positives = 209/518 (40%), Gaps = 22/518 (4%)

Query: 2 AFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASSL 61
A +NTN ++ +Q++L +++ERLSSGLRIN A DDA+G +A+ S L
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 GQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLIQ 121
QA N NDG+ I Q + A++E L V+ + QA + K+IQ +I + ++
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 GLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRI-ATGALI 180
+D + N T +NG +LS + QVGA ++I + +G G
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDN-QMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 181 TASGDISLTFKQVDGVNDVTLESVKISSSAGTGIGVLAEVINKDSNQTGVRAHASVITTS 240
GD+ +FK V G + + + K +G V ++ V A +TT
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 241 DVAVQSGSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQNGRLNLR 300
D N + K A A+ + + + +
Sbjct: 240 DAE-----------NNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTID 288

Query: 301 SLDGRGIEIKTDSVSSGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINV------VS 354
+ G K + +G V S + +N +
Sbjct: 289 TKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKT 348

Query: 355 ASDSQHLGFSTIGFGESQVAETTVNLRDVTGNFNANVKSASGANYNAVIASGNQSL---G 411
++S L ++ TVN + T N + + +G + S
Sbjct: 349 KNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINE 408

Query: 412 SGVTTLRGAMVVIDVAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRD 471
+ + +SA+ +D VRS LG++QN+ S + N+ T N+ +A S+I D
Sbjct: 409 DAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468

Query: 472 VDFAEESANFNKNNILAQSGSYAMSQANTVQQNILRLL 509
D+A E +N +K IL Q+G+ ++QAN V QN+L LL
Sbjct: 469 ADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


21K747_06995K747_06950N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_06995012-1.681239flagellar motor switch protein FliN
K747_11635011-1.002834siderophore-mediated iron transporter
K747_06980012-1.026773dihydroorotase
K747_06970013-1.005166hypothetical protein
K747_06965012-0.734793membrane protein
K747_06960113-0.530706phosphatidylglycerol--membrane-oligosaccharide
K747_069550151.553330bifunctional 5,10-methylene-tetrahydrofolate
K747_06950-1151.414081signal peptidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06995FLGMOTORFLIN992e-30 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 99 bits (249), Expect = 2e-30
Identities = 25/77 (32%), Positives = 47/77 (61%)

Query: 32 LICDYKNLLDMEIVFSAELGSTQIPLLQILRFEKGSVIDLQKPAGESVDTFVNGRVIGKG 91
+ D ++D+ + + ELG T++ + ++LR +GSV+ L AGE +D +NG +I +G
Sbjct: 50 AMQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQG 109

Query: 92 EVMVFERNLAIRLNEIL 108
EV+V +R+ +I+
Sbjct: 110 EVVVVADKYGVRITDII 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11635TONBPROTEIN512e-09 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 50.8 bits (121), Expect = 2e-09
Identities = 23/61 (37%), Positives = 29/61 (47%)

Query: 81 PGAPKPTLAGPQKPPTPPTPPTPPTPPKPIEKPKPEPKPKPKPKPEPKKPNHKHKALKKV 140
P A +P +P P P P P+ KP+PKPKPKPKP K + +K V
Sbjct: 58 PQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPV 117

Query: 141 E 141
E
Sbjct: 118 E 118



Score = 44.2 bits (104), Expect = 2e-07
Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 1/58 (1%)

Query: 91 PQKPPTPPTPPTPPTPPKPIEKPKPEPKPKPKPKPEPKKPNHKHKALKKVEKVEEKKV 148
PQ PP P P P P+ P KP+PK K K +KKV++ ++ V
Sbjct: 58 PQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKP-KPKPVKKVQEQPKRDV 114



Score = 41.1 bits (96), Expect = 3e-06
Identities = 23/63 (36%), Positives = 32/63 (50%)

Query: 98 PTPPTPPTPPKPIEKPKPEPKPKPKPKPEPKKPNHKHKALKKVEKVEEKKVVEEKKEEKK 157
P PP+P+ +P+PEP+P P+P E K K K + KKV E+ K + K
Sbjct: 56 EPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVK 115

Query: 158 IVE 160
VE
Sbjct: 116 PVE 118



Score = 38.4 bits (89), Expect = 2e-05
Identities = 17/58 (29%), Positives = 21/58 (36%), Gaps = 2/58 (3%)

Query: 74 QDPSKNTPGAPKPTLAGPQKPPTPPTPPTPPTPPKPIEKPKPEPKPKP--KPKPEPKK 129
Q +P P P P PKP KPKP+P K +PK + K
Sbjct: 59 QAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKP 116



Score = 36.9 bits (85), Expect = 6e-05
Identities = 42/216 (19%), Positives = 75/216 (34%), Gaps = 36/216 (16%)

Query: 98 PTPPTPPTPPKPIEKPKPEPKPKPKPKPEPKKPNHKHKALKKVEKVEEKKVVEEKKEEKK 157
P PP +P +P EP+P+P+P PEP K VV EK + K
Sbjct: 52 PADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKE---------------APVVIEKPKPKP 96

Query: 158 IVEQKVEQKKIEEKKPVKKEFDPNQLSFLPKEVAPPRQENNKGLDNQTRRDIDELYGEEF 217
+ K +K E+ K K + P N T +
Sbjct: 97 KPKPKPVKKVQEQPKRDVKPVESR----------PASPFENTAPARLTSSTATAATSKPV 146

Query: 218 GDLGTAEKDFIRNNLRDIGRITQKYLEYPQVAAYLGQDGTNAVEFYLHPNGDISDLKIII 277
+ + + RN + YP A L +G V+F + P+G + +++I+
Sbjct: 147 TSVASGPRALSRNQPQ-----------YPARAQALRIEGQVKVKFDVTPDGRVDNVQILS 195

Query: 278 GSEYKMLDDNTLKTIQIAYKDYPRPKTKTLIRIRVR 313
M + ++ + +P + ++ I +
Sbjct: 196 AKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFK 231



Score = 34.2 bits (78), Expect = 4e-04
Identities = 16/57 (28%), Positives = 23/57 (40%)

Query: 74 QDPSKNTPGAPKPTLAGPQKPPTPPTPPTPPTPPKPIEKPKPEPKPKPKPKPEPKKP 130
+P P+P P++ P P P PKP K + +PK KP +P
Sbjct: 65 PEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRP 121



Score = 31.9 bits (72), Expect = 0.002
Identities = 13/53 (24%), Positives = 17/53 (32%)

Query: 75 DPSKNTPGAPKPTLAGPQKPPTPPTPPTPPTPPKPIEKPKPEPKPKPKPKPEP 127
+P P + P P P P K E+PK + KP P
Sbjct: 72 EPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPASP 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06965TYPE3IMSPROT310.003 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 30.5 bits (69), Expect = 0.003
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 4/64 (6%)

Query: 87 LQSYSVMLFFNLLLLTDILGFLPFSIYHHFMASLIFSALFCSSLFLSSPLLGMIALVALS 146
L Y F L+L+ +LPFS S + + +L PLL + AL+A++
Sbjct: 45 LSDYYFEHFSKLMLIPAEQSYLPFSQ----ALSYVVDNVLLEFFYLCFPLLTVAALMAIA 100

Query: 147 SSLL 150
S ++
Sbjct: 101 SHVV 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06950PREPILNPTASE280.042 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 27.8 bits (62), Expect = 0.042
Identities = 10/53 (18%), Positives = 22/53 (41%), Gaps = 10/53 (18%)

Query: 14 WVGTIIIVLLVIFFIAQAFIIPSRSMVGTLYEGDMLFVKKFSYGIPIPKIPWI 66
W+G L ++ ++ S+VG ++ ++ PIP P++
Sbjct: 225 WLG--WQALPIVLLLS--------SLVGAFMGIGLILLRNHHQSKPIPFGPYL 267


22K747_06715K747_11675N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
K747_067151120-3.373385cag pathogenicity island protein
K747_116601119-3.407120hypothetical protein
K747_116651118-3.106059cagX protein
K747_116701118-2.952750hypothetical protein
K747_116751018-2.085204hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_06715PF043351195e-35 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 119 bits (299), Expect = 5e-35
Identities = 44/205 (21%), Positives = 74/205 (36%), Gaps = 10/205 (4%)

Query: 27 KLNKANRTFKRAFYL---SMALNIAAVTSIVMMMPLKKTDIFVYGIDRYTGEFKIVKRSD 83
KL A R+ K A+ + + AL A V ++ + PLK + +V +DR TGE I +
Sbjct: 24 KLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLH 83

Query: 84 A-RQIVNSEAVVDSATSKFVSLLFGYSKNSLRDRKDQLMQYCDVSFQTQAMRMFNENIRQ 142
I EAV + +V G+ + + D +M Q + R + + Q
Sbjct: 84 GDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDNPQ 143

Query: 143 FVDKVRA-EAIISSNIQREKVKNSPLTRLTFFITIKITPDTMENYEYITKKQVTIYYDFA 201
+ A + I + +F +T T TI Y
Sbjct: 144 SPQNILANRTDVFVEI-KRVSFLGGNVAQVYFTKESVTGSNS----TKTDAVATIKYKVD 198

Query: 202 RGNSSQENLIINPFGFKVFDIQITD 226
S + + NP G++V +
Sbjct: 199 GTPSKEVDRFKNPLGYQVESYRADV 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11660TYPE4SSCAGX2662e-90 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 266 bits (680), Expect = 2e-90
Identities = 143/154 (92%), Positives = 145/154 (94%), Gaps = 1/154 (0%)

Query: 1 MEQAFFKKIVGCFCLGYLFLSSVIEAAP-DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 59
M QAFFKKIVGCFCLGYLFLSS IEA DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS
Sbjct: 1 MGQAFFKKIVGCFCLGYLFLSSAIEAVALDIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 60

Query: 60 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 119
LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR
Sbjct: 61 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 120

Query: 120 DYQEFLKTKKLIVDAPDPKELEEQKKSSRKRKRS 153
DYQEFLKTKKLIVDAPDPKELEEQKK+ K K +
Sbjct: 121 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEA 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11665TYPE4SSCAGX5570.0 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 557 bits (1435), Expect = 0.0
Identities = 333/336 (99%), Positives = 335/336 (99%)

Query: 1 MSNPQNLSNNKNLSEFIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVKQ 60
MSNPQNLSNNKNLSE IKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAV+Q
Sbjct: 187 MSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVRQ 246

Query: 61 RAKDKINIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAY 120
RAKDKI+IKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAY
Sbjct: 247 RAKDKISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAY 306

Query: 121 LTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKE 180
LTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKE
Sbjct: 307 LTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKE 366

Query: 181 KIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFT 240
KIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFT
Sbjct: 367 KIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFT 426

Query: 241 YFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVT 300
YFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVT
Sbjct: 427 YFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVT 486

Query: 301 VINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 336
VINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK
Sbjct: 487 VINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 522


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
K747_11675IGASERPTASE422e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 42.4 bits (99), Expect = 2e-05
Identities = 40/259 (15%), Positives = 85/259 (32%), Gaps = 36/259 (13%)

Query: 118 TPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDC 177
P ++ + + ++KT + ++ T + ++ +EAK +VKA
Sbjct: 1023 APVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQT 1082

Query: 178 VSQAKTEAEKKECEKLLTPEAKKLLEQQALDCLKSAKTDEERKKCLKDLPKDLQKKVLAK 237
A++ +E KE + T E A ++E K AK
Sbjct: 1083 NEVAQSGSETKETQTTETKET--------------ATVEKEEK---------------AK 1113

Query: 238 ESLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKE 297
+ ++ KQE + + P+A+ E + ++K SQ T A+ ++
Sbjct: 1114 VETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEP---QSQTNTTADTEQ 1170

Query: 298 CEKLLTPEAKKLLEQQALDCLKSAKTDEERKKCLKDLPKDLQKKVLAKESLKAYKDCVSR 357
K + ++ + + + + P Q V ++ S K
Sbjct: 1171 PAKETSSNVEQPVTEST----TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRS 1226

Query: 358 ARNEKEKQECEKLLTPEAK 376
R+ E + +
Sbjct: 1227 VRSVPHNVEPATTSSNDRS 1245



Score = 36.6 bits (84), Expect = 9e-04
Identities = 35/269 (13%), Positives = 76/269 (28%), Gaps = 18/269 (6%)

Query: 357 RARNEKEKQECEKLLTPEAKKLLEEAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKKL 416
+ + + TP + + S +R + + +
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEI---ARVDEAPVPPPAPATPSETTETV 1040

Query: 417 LEEAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKNLLEQQALDCLKSAKTEAEKKRCV 476
E +K+ K + A E Q E ++ Q + +S E +
Sbjct: 1041 AENSKQESKTVEKNEQDAT-ETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTE 1099

Query: 477 KDLPKDLQKKVLAKESVKAYLDCVSRARNEKEKKECEKLLTPEAKKLLEEAKESLKAYKD 536
++K+ AK + + K+E + + P+A+ E
Sbjct: 1100 TKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVN----- 1154

Query: 537 CLSQARNEEERRACEKLLTPEARKLLEQEVKKSVKAYLDCVSRARNEKEKQECEKLLTPE 596
+ + +++ A + E +EQ V + N E + TP
Sbjct: 1155 -IKEPQSQTNTTADTEQPAKETSSNVEQPVTE------STTVNTGNSVV--ENPENTTPA 1205

Query: 597 ARKFLAKQVLSCLEKARNEEERKACLKNI 625
+ S K R+ ++ N+
Sbjct: 1206 TTQPTVNSESSNKPKNRHRRSVRSVPHNV 1234



Score = 35.4 bits (81), Expect = 0.002
Identities = 35/242 (14%), Positives = 86/242 (35%), Gaps = 5/242 (2%)

Query: 65 KNAKTDEERKKCLKDLPKDLQSDILAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKKL 124
+ ++ E + + P + ++ + + + EK E + T +
Sbjct: 1008 PSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNRE 1067

Query: 125 LEEEAKESVKAYLDC--VSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDCVSQAK 182
+ +EAK +VKA V+Q+ +E ++ + + +K E+AK + +
Sbjct: 1068 VAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS 1127

Query: 183 TEAEKKECEKLLTPEAKKLLEQQALDCLKSAKTDEERKKCLKDLPKDLQKKV---LAKES 239
+ K+E + + P+A+ E +K ++ + K+ V + + +
Sbjct: 1128 QVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTEST 1187

Query: 240 LKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECE 299
+ V + + E+ + + SV++ V A T + +
Sbjct: 1188 TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTV 1247

Query: 300 KL 301
L
Sbjct: 1248 AL 1249



Score = 33.5 bits (76), Expect = 0.007
Identities = 23/160 (14%), Positives = 53/160 (33%), Gaps = 2/160 (1%)

Query: 264 TPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKLLEQQALDCLKSAKT 323
TP E + ++ Q TE + E ++ Q + +S
Sbjct: 1032 TPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE 1091

Query: 324 DEERKKCLKDLPKDLQKKVLAKESLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEAK 383
+E + ++K+ AK + ++ KQE + + P+A+ E
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREND- 1150

Query: 384 KSLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEAKKS 423
K+ S+ + ++ K + ++ + E+
Sbjct: 1151 -PTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTV 1189



Score = 32.3 bits (73), Expect = 0.014
Identities = 32/239 (13%), Positives = 80/239 (33%), Gaps = 8/239 (3%)

Query: 34 KARNEKEKKECEKLLTPEAKKKLEQQVLDCLKNAKTDEERKKCLKDLPKD--LQSDILAK 91
+ NE+ + E + P A + +N+K + + + + + Q+ +AK
Sbjct: 1011 PSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAK 1070

Query: 92 KSLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKE 151
++ K + E ++ T E K+ E +E K + + +
Sbjct: 1071 EAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVS 1130

Query: 152 CEKLLTPEAKKKLEEAKKSVKAYL--DCVSQAKTEAEKKECEKLLTPEAKKLLEQQALDC 209
++ + + + E A+++ + SQ T A+ ++ K + ++ + +
Sbjct: 1131 PKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTEST--- 1187

Query: 210 LKSAKTDEERKKCLKDLPKDLQKKVLAKESLKAYKDCVSRARNEKEKQECEKLLTPEAK 268
+ + P Q V ++ S K R+ E + +
Sbjct: 1188 -TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRS 1245



Score = 31.6 bits (71), Expect = 0.028
Identities = 25/157 (15%), Positives = 60/157 (38%), Gaps = 5/157 (3%)

Query: 16 QKELLADMSVKAYKDCVSKARNEKEKKECEKLLTPEAKKKLE--QQVLDCLKNAKTDEER 73
E +A+ S + K ++ E + + EAK ++ Q + ++ +E
Sbjct: 1036 TTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKET 1095

Query: 74 KKCLKDLPKDLQSDILAKKSLKAYKDCVSRARNEKEKQECEKLLTPEAKKLLEEEAKESV 133
+ ++ + AK + ++ KQE + + P+A+ E + ++
Sbjct: 1096 QTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNI 1155

Query: 134 KAYLDCVSQAKTEAEKKECEKLLTPEAKKKLEEAKKS 170
K SQ T A+ ++ K + ++ + E+
Sbjct: 1156 KEP---QSQTNTTADTEQPAKETSSNVEQPVTESTTV 1189



 
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