PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
GenomeSat464.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in CP002071 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1HPSAT_00300HPSAT_00405Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_003004243.034917urease accessory protein
HPSAT_003055242.714298urease accessory protein (ureF)
HPSAT_003104232.438116urease accessory protein UreE
HPSAT_003153202.377112urea transporter
HPSAT_003201181.878172hypothetical protein
HPSAT_003251172.460255urease subunit beta
HPSAT_00330-2101.489903urease subunit alpha
HPSAT_00335-1111.845998*lipoprotein signal peptidase
HPSAT_003402122.468048phosphoglucosamine mutase
HPSAT_003453152.65985830S ribosomal protein S20
HPSAT_003502141.844444peptide chain release factor 1
HPSAT_003553151.693916hypothetical protein
HPSAT_003603141.629503outer membrane protein HorA
HPSAT_003653141.036013hypothetical protein
HPSAT_00370-2140.325717hypothetical protein
HPSAT_00375-2110.261747methyl-accepting chemotaxis transducer (tlpC)
HPSAT_003800120.23386430S ribosomal protein S9
HPSAT_003850120.42700950S ribosomal protein L13
HPSAT_003901110.756094hypothetical protein
HPSAT_00395010-0.248822Malate:quinone oxidoreductase (Malate
HPSAT_00400010-0.336264hypothetical protein
HPSAT_00405212-0.607304RNA polymerase sigma factor RpoD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00325UREASE10430.0 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 1043 bits (2700), Expect = 0.0
Identities = 356/569 (62%), Positives = 445/569 (78%), Gaps = 4/569 (0%)

Query: 3 KISRKEYASMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSN-NP 61
++SR YA+M+GPT GDKVRL DT+L EVE D+T +GEE+KFGGGK +R+GM QS
Sbjct: 4 RMSRAAYANMFGPTVGDKVRLADTELFIEVEKDFTTHGEEVKFGGGKVIRDGMGQSQVTR 63

Query: 62 SKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEAL 121
+D +ITNALI+D+ GI KADIG+KDG+IA IGK GN DMQ GV + VGP TE +
Sbjct: 64 EGGAVDTVITNALILDHWGIVKADIGLKDGRIAAIGKAGNPDMQPGV--TIIVGPGTEVI 121

Query: 122 AGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKF 181
AGEG IVTAGG+D+HIHFI PQQI A SG+T M+GGGTGPA GT ATT TPG ++
Sbjct: 122 AGEGKIVTAGGMDSHIHFICPQQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIAR 181

Query: 182 MLRAAEEYSMNIGFLAKGNASNDASLADQIEAGAIGLKIHEDWGTTPSAINHALDVADKY 241
M+ AA+ + MN+ F KGNAS +L + + GA LK+HEDWGTTP+AI+ L VAD+Y
Sbjct: 182 MIEAADAFPMNLAFAGKGNASLPGALVEMVLGGATSLKLHEDWGTTPAAIDCCLSVADEY 241

Query: 242 DVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTYHTEGAGGGHAPDIIKVAGEHNILPASTN 301
DVQV IHTDTLNE+G VEDT+AAI GRT+H YHTEGAGGGHAPDII++ G+ N++P+STN
Sbjct: 242 DVQVMIHTDTLNESGFVEDTIAAIKGRTIHAYHTEGAGGGHAPDIIRICGQPNVIPSSTN 301

Query: 302 PTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSS 361
PT P+TVNT AEH+DMLMVCHHL +I ED+ FA+SRIR +TIAAED LHD+G FSI SS
Sbjct: 302 PTRPYTVNTLAEHLDMLMVCHHLSPTIPEDIAFAESRIRKETIAAEDILHDIGAFSIISS 361

Query: 362 DSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISE 421
DSQAMGRVGEV RTWQTADK K++ GRLKEE GDNDNFR+KRY++KYTINPAIAHG+S
Sbjct: 362 DSQAMGRVGEVAIRTWQTADKMKRQRGRLKEETGDNDNFRVKRYIAKYTINPAIAHGLSH 421

Query: 422 YVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAH 481
+GS+EVGK ADLVLW+PAFFGVKP+M++ GG IA + MGD NASIPTPQPV+YR MF
Sbjct: 422 EIGSLEVGKRADLVLWNPAFFGVKPDMVLLGGTIAAAPMGDPNASIPTPQPVHYRPMFGA 481

Query: 482 HGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCR-NITKKDMQFNDTTAHIEVNP 540
+G+++ ++++TFVSQA+ D G+ LG+ ++++ V+N R I K M N T HIEV+P
Sbjct: 482 YGRSRTNSSVTFVSQASLDAGLAGRLGVAKELVAVQNTRGGIGKASMIHNSLTPHIEVDP 541

Query: 541 ETYHVFVDGKEVTSKPANKVSLAQLFSIF 569
ETY V DG+ +T +PA + +AQ + +F
Sbjct: 542 ETYEVRADGELLTCEPATVLPMAQRYFLF 570


2HPSAT_01545HPSAT_01625Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_01545215-0.042315hypothetical protein
HPSAT_01550113-0.421534hypothetical protein
HPSAT_01555012-1.341561type II restriction enzyme
HPSAT_01560010-0.678003DNA methylase
HPSAT_015651120.239146hypothetical protein
HPSAT_01570013-0.249407conserved hypothetical ATP-binding protein
HPSAT_01575116-0.700916nitrite extrusion protein (narK)
HPSAT_01580318-1.257439putative heme iron utilization protein
HPSAT_01585416-1.655953arginyl-tRNA synthetase
HPSAT_01590318-1.547007Sec-independent protein translocase protein
HPSAT_01595215-1.158373guanylate kinase
HPSAT_01600114-1.301062poly E-rich protein
HPSAT_01605-210-1.952927hypothetical protein
HPSAT_01610-210-2.382568nuclease NucT
HPSAT_01615010-2.314789outer membrane protein HorC
HPSAT_01620113-2.355434flagellar basal body L-ring protein
HPSAT_01625213-2.029733CMP-N-acetylneuraminic acid synthetase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01575TCRTETA447e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 44.0 bits (104), Expect = 7e-07
Identities = 55/271 (20%), Positives = 103/271 (38%), Gaps = 16/271 (5%)

Query: 28 LILSGSLTPHQSFQLGIAVLMGYVFGSFLIQFLSPLMSLESIAKISFGLIALSFLICYFD 87
L+ S +T H L + LM + L LS + +S A+ + I
Sbjct: 35 LVHSNDVTAHYGILLALYALMQFACAPVLGA-LSDRFGRRPVLLVSLAGAAVDYAI--MA 91

Query: 88 SIPFFW-LWIWRFIAGVASSALMILVAPLSLPYVKENKRALVGGFIFSAVGIGSVFSGFV 146
+ PF W L+I R +AG+ + A + ++RA GF+ + G G V +
Sbjct: 92 TAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVL 150

Query: 147 LPWISSYNIKWAWIFLGGSCLIAFVLSLTGLKN-HSLKKKSVKKEESAFKIPFHL----- 200
+ ++ + + F+ L H +++ +++E F
Sbjct: 151 GGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMT 210

Query: 201 ---WLLLISCALNAIGFLPHTLFWVDYLIRHLNISPAIAGTSWAFFG-FGATLGSLISGP 256
L+ + + +G +P L WV + + G S A FG + ++I+GP
Sbjct: 211 VVAALMAVFFIMQLVGQVPAAL-WVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGP 269

Query: 257 MAQKLGAKNANIFILILKSIACFLPIFFHQI 287
+A +LG + A + +I L F +
Sbjct: 270 VAARLGERRALMLGMIADGTGYILLAFATRG 300


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01595PF05272290.011 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.011
Identities = 9/18 (50%), Positives = 11/18 (61%)

Query: 8 LILSGPSGAGKSTLTKYL 25
++L G G GKSTL L
Sbjct: 599 VVLEGTGGIGKSTLINTL 616


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01600IGASERPTASE654e-13 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 65.1 bits (158), Expect = 4e-13
Identities = 39/227 (17%), Positives = 77/227 (33%), Gaps = 17/227 (7%)

Query: 162 LPTLNDQEEKEEVKKEIKETPQEEEKPKDDEIQEGETLKDEEVSKELETQE----ELEIP 217
P+ + E K+E K + E+ + Q E K+ + + + TQ +
Sbjct: 1032 TPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE 1091

Query: 218 KEETQEQAKEQESIKEKMQEIKEEKQGKTQDSPSAQELEAMQELVKEIQENSNGQEDKKE 277
+ETQ ++ + EK ++ K E + + ++ QE + +Q + +
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP 1151

Query: 278 TQELETPQETEKQELETPQELETQESAEIPQENTETPQETPQELEIQESAETQES-AEIP 336
T ++ PQ +T +A+ Q ET Q + + T S E P
Sbjct: 1152 TVNIKEPQS------------QTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENP 1199

Query: 337 QETEKQELETPQETAQENKETPQESAEIPQETEKQELETPQETQQEN 383
+ T + + NK + + E T +
Sbjct: 1200 ENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRST 1246



Score = 65.1 bits (158), Expect = 5e-13
Identities = 47/289 (16%), Positives = 90/289 (31%), Gaps = 11/289 (3%)

Query: 211 QEELEIPKEETQEQAKEQESIKEKMQEIKEEKQGKTQ---DSPSAQELEAMQELVKEIQE 267
E+ + T E+ + P A +
Sbjct: 985 VEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENS 1044

Query: 268 NSNGQEDKKETQELETP--QETEKQELETPQELETQESAEIPQENTETPQETPQELEIQE 325
+ +K Q+ Q E + ++ E+ Q +ET + E +
Sbjct: 1045 KQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETA 1104

Query: 326 SAETQESAEIPQETEKQELETPQETAQENKETPQESAEIPQ-ETEKQELETPQETQQENT 384
+ E +E A++ ETEK + E P+ T+Q + + Q PQ E ++ T + ++
Sbjct: 1105 TVEKEEKAKV--ETEKTQ-EVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQ 1161

Query: 385 ETPQESAEIPQETEKQELETP--QETPQENTETPQESAETPQEKETQKLENQEDHYESIE 442
E P + +E P + T + E+ E TQ N E +
Sbjct: 1162 TNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKN 1221

Query: 443 DIPEPVMAQAMGEALPFLNESVAKTPNNENDTETPKESVIKTPQEKEES 491
V + + + T + T T +V+ + K +
Sbjct: 1222 RHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQF 1270



Score = 64.3 bits (156), Expect = 8e-13
Identities = 55/287 (19%), Positives = 105/287 (36%), Gaps = 36/287 (12%)

Query: 241 EKQGKTQDSPSAQELEAMQELVKEIQENSNGQEDKKETQELETPQETEKQELETPQELET 300
EK+ +T D+ + +Q V + N+ +E+ E
Sbjct: 986 EKRNQTVDTTNITTPNNIQADVPSVPSNN--------------------EEIARVDEAPV 1025

Query: 301 QESAE-IPQENTETPQETPQELEIQESAETQESAEIPQETEKQELETPQETAQENKETPQ 359
A P E TET E + QES +++ + ET Q E +E K Q
Sbjct: 1026 PPPAPATPSETTETVAENSK----QESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQ 1081

Query: 360 ESAEIPQETEKQELETPQETQQENTETPQESAEIPQETEKQELETPQETPQENTETPQES 419
+ E+ Q E + Q T+ + T T ++ +E K E E QE P+ ++ +
Sbjct: 1082 -TNEVAQS--GSETKETQTTETKETATVEK-----EEKAKVETEKTQEVPKVTSQVSPKQ 1133

Query: 420 AETPQEKETQKLENQEDHYESIEDIPEPVMAQAMGEALPFLNESVAKTPNNENDTETPKE 479
++ + + + D +I++ A E S + P E+ T
Sbjct: 1134 EQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGN 1193

Query: 480 SVIKTPQEKEESDKNSSPLELRLNLQDLLKSLNQESLKSLLENKTLS 526
SV++ P+ + + + K+ ++ S++S+ N +
Sbjct: 1194 SVVENPENTTPATTQP---TVNSESSNKPKNRHRRSVRSVPHNVEPA 1237



Score = 45.8 bits (108), Expect = 4e-07
Identities = 28/191 (14%), Positives = 67/191 (35%), Gaps = 5/191 (2%)

Query: 146 DLKALAQEEPNNEEQLLPTLNDQEEKEEVKKEIKET-PQEEEKPKDDEIQEGETLKDEEV 204
+ A +E + + E + E KET E ++ E +E ++ E+
Sbjct: 1060 ETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKT 1119

Query: 205 SKELETQEELEIPKEETQEQAKEQESIKEKMQEIKEEKQGKTQDSPSAQELEAMQELVKE 264
+ + ++ +E+++ + E +E + ++ ++ + E A +
Sbjct: 1120 QEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNV 1179

Query: 265 IQENSNGQEDKKETQELETPQETEKQELETPQELETQESAEIPQE-NTETPQETPQELEI 323
Q + +E P+ T T Q ES+ P+ + + + P +E
Sbjct: 1180 EQPVTESTTVNTGNSVVENPENTTP---ATTQPTVNSESSNKPKNRHRRSVRSVPHNVEP 1236

Query: 324 QESAETQESAE 334
++ S
Sbjct: 1237 ATTSSNDRSTV 1247



Score = 37.7 bits (87), Expect = 1e-04
Identities = 28/242 (11%), Positives = 61/242 (25%), Gaps = 7/242 (2%)

Query: 148 KALAQEEPNNEEQLLPTLNDQEEKEEVKKEIKETPQEEEKPKDDEIQEGETLKDEEVSKE 207
A + E + EKEE K E QE K + E + + E
Sbjct: 1085 VAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAE 1144

Query: 208 LETQEELEIPKEETQEQAKEQESIKEKMQEIKEEKQGKTQDSPSAQELEAMQELVKEIQE 267
+ + + +E Q Q ++ +E + +S + ++ E +
Sbjct: 1145 PARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTP 1204

Query: 268 NSNGQEDKKETQELETPQETEKQELETPQELETQESAEIPQENTETPQETPQELEIQESA 327
+ E+ + + E A + T +
Sbjct: 1205 ATTQPTVNSESSN----KPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAV 1260

Query: 328 ETQESAEIPQETEKQELETPQETAQENKETPQESA---EIPQETEKQELETPQETQQENT 384
+ A+ Q +Q + + + ++T
Sbjct: 1261 LSDARAKAQFVALNVGKAVSQHISQLEMNNEGQYNVWVSNTSMNKNYSSSQYRRFSSKST 1320

Query: 385 ET 386
+T
Sbjct: 1321 QT 1322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01620FLGLRINGFLGH1913e-63 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 191 bits (486), Expect = 3e-63
Identities = 51/172 (29%), Positives = 84/172 (48%), Gaps = 18/172 (10%)

Query: 56 GERPLFADRRAMKPNDLITIIVSEKASANYSSS----KDYKSASGGNSTPPRLTYNGLDE 111
G +PLF DRR D +TI++ E SA+ SSS +D K+ G ++ P L GL
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYL--QGLFG 118

Query: 112 RKKQEAQYLDDKNNYNFTKSSNNTNFKGGGSQKKSEDLEIVLSARIIKVLENGNYFIYGN 171
+ + + S F G G S L+ + +VL NGN + G
Sbjct: 119 NARADVEA------------SGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGE 166

Query: 172 KEVLVDGEKQILKVSGVIRPYDIERNNTIQSKFLADAKIEYTNLGHLSDSNK 223
K++ ++ + ++ SGV+ P I +NT+ S +ADA+IEY G+++++
Sbjct: 167 KQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN 218


3HPSAT_02185HPSAT_02210Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_02185-214-3.117550dihydroorotate dehydrogenase 2
HPSAT_02190-214-3.392404polyphosphate kinase
HPSAT_02195-117-4.141209*hypothetical protein
HPSAT_02200-117-4.048356Type I restriction/modification specificity
HPSAT_02205-115-3.095877type I restriction enzyme M protein
HPSAT_02210-115-3.035248type I restriction enzyme R protein (hsdR)
4HPSAT_02260HPSAT_02365Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_02260013-3.481815molybdenum ABC transporter ModA
HPSAT_02265011-3.716704molybdenum ABC transporter ModB
HPSAT_02270-18-2.177602molybdenum ABC transporter ModD
HPSAT_02275-18-2.457509glutamyl-tRNA synthetase
HPSAT_02280-111-3.100137outer membrane protein (omp22)
HPSAT_02285-113-3.029177type II R-M system methyltransferase
HPSAT_02290-115-2.672930type II adenine specific methyltransferase
HPSAT_02295015-1.877618hypothetical protein
HPSAT_02300517-1.696175type II adenine specific DNA methyltransferase
HPSAT_02305616-0.877340type II restriction endonuclease
HPSAT_02310315-0.535523type II DNA modification enzyme
HPSAT_02315316-0.354047hypothetical protein
HPSAT_023204170.749213catalase-like protein
HPSAT_023254141.950679outer membrane protein HofC
HPSAT_023303131.388155putative Outer membrane protein
HPSAT_023353131.524636hypothetical protein
HPSAT_023403141.836716hypothetical protein
HPSAT_023453142.141327Holliday junction resolvase
HPSAT_023502132.234993iron-regulated outer membrane protein
HPSAT_023553152.867361catalase
HPSAT_023602153.014007hypothetical protein
HPSAT_023652172.520897hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_02270PF05272300.009 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.009
Identities = 11/23 (47%), Positives = 14/23 (60%)

Query: 30 VVALLGESGAGKSTILRILAGLE 52
V L G G GKST++ L GL+
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_02295TCRTETOQM1964e-57 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 196 bits (501), Expect = 4e-57
Identities = 115/461 (24%), Positives = 189/461 (40%), Gaps = 67/461 (14%)

Query: 3 NIRNIAVIAHVDHGKTTLVDGLLSQSGTFSEREKVDE--RVMDSNDLERERGITILSKNT 60
I NI V+AHVD GKTTL + LL SG +E VD+ D+ LER+RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 61 AIYYKGTKINIIDTPGHADFGGEVERVLKMVDGVLLLVDAQEGVMPQTKFVVKKALSFGI 120
+ ++ TK+NIIDTPGH DF EV R L ++DG +LL+ A++GV QT+ + GI
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 121 CPIVVVNKIDKPAAEPDRVVDEVFDLF---------VAMGASDKQLDFPV-----VYAAA 166
I +NKID+ + V ++ + V + + +F
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 167 RDGYAMKSLDDE----------------------------KKNL--EPLFETILEHVPSP 196
D K + + K N+ + L E I S
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSS 241

Query: 197 SGSVDEPLQMQIFTLDYDNYVGKIGIARVFNGSVKKNESVLLMKSDGSKENGRITKLIGF 256
+ L ++F ++Y ++ R+++G + +SV + KE +IT++
Sbjct: 242 THRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRI----SEKEKIKITEMYTS 297

Query: 257 LGLARTEIENAYAGDIVAIAG--FNAMDV-GDSVVDPANPMPLDPMHLEEPTMSVYFAVN 313
+ +I+ AY+G+IV + V GD+ + P +P P + +
Sbjct: 298 INGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENP----LPLLQTTVEPS 353

Query: 314 DSPLAGLEGKHVTANKLKDRLLKEMQTNIAMKCEEMGEGKFKVSGRGELQITILAENLRR 373
+ + D LL+ + + +S G++Q+ + L+
Sbjct: 354 KPQQREMLLDALLEISDSDPLLRYYVDSAT--------HEIILSFLGKVQMEVTCALLQE 405

Query: 374 E-GFEFSISRPEVIIKEENGVKCEPFEHLVIDTPQDFSGAI 413
+ E I P VI E K E H+ + P F +I
Sbjct: 406 KYHVEIEIKEPTVIYMERPLKKAEYTIHIEVP-PNPFWASI 445



Score = 41.8 bits (98), Expect = 8e-06
Identities = 20/80 (25%), Positives = 30/80 (37%), Gaps = 1/80 (1%)

Query: 396 EPFEHLVIDTPQDFSGAIIERLGKRKAEMKAMNPMSDGYTRLEFEIPARGLIGYRSEFLT 455
EP+ I PQ++ K A + + + L EIPAR + YRS+
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCIQEYRSDLTF 595

Query: 456 DTKGEGVMNHSFLEFRPFSG 475
T G V + +G
Sbjct: 596 FTNGRSVCLTELKGYHVTTG 615


5HPSAT_02775HPSAT_02800Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_02775220-1.845662molybdopterin converting factor, subunit 2
HPSAT_02780519-3.299732molybdenum cofactor biosynthesis protein MogA
HPSAT_02785417-3.705751molybdenum cofactor biosynthesis protein MoaC
HPSAT_02790313-3.618576flagellar sheath adhesin
HPSAT_02795313-2.875865outer membrane protein (omp18)
HPSAT_02800313-2.750637Transposon IS607 OrfA, integrase-resolvase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_02790PF05211403e-146 Neuraminyllactose-binding hemagglutinin
		>PF05211#Neuraminyllactose-binding hemagglutinin

Length = 260

Score = 403 bits (1037), Expect = e-146
Identities = 252/260 (96%), Positives = 255/260 (98%)

Query: 1 MKANNHFKDFAWKKCLLGVSVVALLVGCSPHIIETNEVTLKLNYHPASEKVQALDEKILL 60
MKANNHFKDFAWKKCLLG SVVALLVGCSPHIIETNEV LKLNYHPASEKVQALDEKILL
Sbjct: 1 MKANNHFKDFAWKKCLLGASVVALLVGCSPHIIETNEVALKLNYHPASEKVQALDEKILL 60

Query: 61 LRPAFQYSDNIAKEYENKFKNQTTLKVEQILQNQGYKVINVDSSDKDDFSFAQKKEGYLA 120
LRPAFQYSDNIAKEYENKFKNQTTLKVEQILQNQGYKVINVDSSDKDDFSFAQKKEGYLA
Sbjct: 61 LRPAFQYSDNIAKEYENKFKNQTTLKVEQILQNQGYKVINVDSSDKDDFSFAQKKEGYLA 120

Query: 121 VAMSGEIVLRPDPKRTIQKKSEPGLLFSTGLDKMEGVLISAGFIKVTILEPMSGESLDSF 180
VAM+GEIVLRPDPKRTIQKKSEPGLLFSTGLDKMEGVLI AGF+KVTILEPMSGESLDSF
Sbjct: 121 VAMNGEIVLRPDPKRTIQKKSEPGLLFSTGLDKMEGVLIPAGFVKVTILEPMSGESLDSF 180

Query: 181 TMDLSELDIQEKFLKTTHSSHSGGLVSTMVKGTDNSNDAIKSALNKIFTSIMQEIDKKLT 240
TMDLSELDIQEKFLKTTHSSHSGGLVSTMVKGTDNSNDAIKSALNKIF +IMQEIDKKLT
Sbjct: 181 TMDLSELDIQEKFLKTTHSSHSGGLVSTMVKGTDNSNDAIKSALNKIFANIMQEIDKKLT 240

Query: 241 QKNLESYQKDAKELKNKRNR 260
QKNLESYQKDAKELK KRNR
Sbjct: 241 QKNLESYQKDAKELKGKRNR 260


6HPSAT_03120HPSAT_03215Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_03120314-1.791989putative nucleotide phosphoribosyltransferase
HPSAT_03125316-2.260928hypothetical protein
HPSAT_03130114-2.540372hypothetical protein
HPSAT_03150013-1.903675hypothetical protein
HPSAT_03165-112-0.808285hypothetical protein
HPSAT_03170-1120.271297hypothetical protein
HPSAT_031751121.911041tRNA(Ile)-lysidine synthase
HPSAT_031801142.471776transcriptional regulator
HPSAT_031852142.417697outer membrane protein
HPSAT_031902142.636549putative Outer membrane protein
HPSAT_031953142.491898anaerobic C4-dicarboxylate transporter
HPSAT_032001120.675991L-asparaginase II
HPSAT_03205215-0.843244hypothetical protein
HPSAT_03210214-0.138978hypothetical protein
HPSAT_03215313-0.908177hypothetical protein
7HPSAT_03300HPSAT_03370Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_033002111.571909hypothetical protein
HPSAT_033053122.651265DNA gyrase subunit A
HPSAT_033102142.543149diacylglycerol kinase
HPSAT_033151142.512202hypothetical protein
HPSAT_033203153.472142hypothetical protein
HPSAT_033253143.489727N-methylhydantoinase
HPSAT_033302133.833562hydantoin utilization protein A
HPSAT_033351112.410104putative outer membrane protein
HPSAT_03340-1102.065270short-chain fatty acids transporter
HPSAT_033452111.434384succinyl-CoA-transferase subunit B
HPSAT_033501100.956412succinyl-CoA-transferase subunit A
HPSAT_033551100.510341acetyl-CoA acetyltransferase
HPSAT_0336019-0.134278hypothetical protein
HPSAT_033653100.437041iron(II) transport protein
HPSAT_033705140.723022iron(III) dicitrate transport protein
8HPSAT_03965HPSAT_04000Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_03965212-1.413963hypothetical protein
HPSAT_03970112-1.278207membrane protein
HPSAT_03975114-0.066306diaminopimelate epimerase
HPSAT_039802130.117029hypothetical protein
HPSAT_03985215-0.266820hypothetical protein
HPSAT_039902111.947833hypothetical protein
HPSAT_039953142.89847330S ribosomal protein S21
HPSAT_040002142.4941993-ketoacyl-(acyl-carrier-protein) reductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04000DHBDHDRGNASE1241e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (312), Expect = 1e-36
Identities = 78/250 (31%), Positives = 113/250 (45%), Gaps = 11/250 (4%)

Query: 5 GKNVLITGASKGIGAEIAKTLASMGLKVWINYRSNAEVADALKNELEEKGYKAAVIKFDA 64
GK ITGA++GIG +A+TLAS G + N E + + + L+ + A D
Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHI-AAVDYNPEKLEKVVSSLKAEARHAEAFPADV 66

Query: 65 ASESDFVEAIQTIVQSDGGLSYLVNNAGVVRDKLAIKMKTEDFHHVIDNNLTSAFIGCRE 124
+ E I + G + LVN AGV+R L + E++ N T F R
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 125 ALKVMSKSRFGSVVNIASIIGERGNMGQTNYSASKGGMIAMSKSFAYEGALRNIRFNSVT 184
K M R GS+V + S Y++SK + +K E A NIR N V+
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 185 PGFIETDMNANL--KDELKADYVK--------NIPLNRLGAAKEVAEAVAFLLSDHSSYI 234
PG ETDM +L + +K IPL +L ++A+AV FL+S + +I
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 235 TGETLKVNGG 244
T L V+GG
Sbjct: 247 TMHNLCVDGG 256


9HPSAT_04050HPSAT_04170Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_04050315-0.79114850S ribosomal protein L31
HPSAT_04055416-0.813166transcription termination factor Rho
HPSAT_04060520-2.217478glutamate racemase
HPSAT_04065824-3.007225cag pathogenicity island protein (cagA, cag26)
HPSAT_040701530-5.985614hypothetical protein
HPSAT_040751429-6.248872cag pathogenicity island protein (cagB)
HPSAT_040801031-7.497920cag pathogenicity island protein (cagC, cag25)
HPSAT_040851031-7.959260hypothetical protein
HPSAT_040901430-5.846162hypothetical protein
HPSAT_040951231-5.623341cag pathogenicity island protein (cagQ, cag14)
HPSAT_041001330-5.702652hypothetical protein
HPSAT_041051030-5.020307hypothetical protein
HPSAT_041101028-4.785855CAG pathogenicity island protein 13
HPSAT_041151021-3.748228CAG pathogenicity island protein 12
HPSAT_04120922-3.840761cag pathogenicity island protein (cagU, cag11)
HPSAT_04125921-3.868895cag pathogenicity island protein (cagV, cag10)
HPSAT_041301020-3.659760cag pathogenicity island protein (cagW, cag9)
HPSAT_041351018-3.310227cag pathogenicity island protein (cagX, cag8)
HPSAT_041401017-3.000778cag pathogenicity island protein (cagY, cag7)
HPSAT_04145918-2.787599hypothetical protein
HPSAT_04150918-2.750985cag pathogenicity island protein (cagZ, cag6)
HPSAT_04155718-2.401963cag pathogenicity island protein cag alpha
HPSAT_04160415-1.952977cag pathogenicity island protein (cag beta,
HPSAT_04165214-1.937468cag pathogenicity island protein (cag4)
HPSAT_04170212-1.731737cag pathogenicity island protein (cag delta,
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04050PF01206270.004 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 26.6 bits (59), Expect = 0.004
Identities = 7/22 (31%), Positives = 12/22 (54%)

Query: 19 SGKEIEVLSTKPEMRIDISSFC 40
+G+ + V++T P D SF
Sbjct: 31 AGEVLYVMATDPGSVKDFESFS 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04065TYPE4SSCAGA16640.0 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 1664 bits (4310), Expect = 0.0
Identities = 916/1168 (78%), Positives = 1010/1168 (86%), Gaps = 36/1168 (3%)

Query: 7 IKNPYLDQTP-SQTAFDPQQFINNLQVAFIKVDSAVASFDPDQKPIVDKNDRDNRQAFNK 65
+ N +DQ P ++ AF+PQQFINNLQVAF+KVD+AVAS+DPDQKPIVDKNDRDNRQAF
Sbjct: 1 MTNETIDQQPQTEAAFNPQQFINNLQVAFLKVDNAVASYDPDQKPIVDKNDRDNRQAFEG 60

Query: 66 ISQLREEYANKAIKNPTKKNQYFSEFIGKSNDLINKDNLIDTDSSTKSFEKFGTERYQIF 125
ISQLREEY+NKAIKNPTKKNQYFS+FI KSNDLINKDNLID +SSTKSF+KFG +RY+IF
Sbjct: 61 ISQLREEYSNKAIKNPTKKNQYFSDFINKSNDLINKDNLIDVESSTKSFQKFGDQRYRIF 120

Query: 126 MNWVSHQKDPSKINTQKIRNFMENIIQPPISDDKEKAEFLRSAKQSFAGIIIGNQIRSDE 185
+WVSHQ DPSKINT+ IRNFMENIIQPPI DDKEKAEFL+SAKQSFAGIIIGNQIR+D+
Sbjct: 121 TSWVSHQNDPSKINTRSIRNFMENIIQPPILDDKEKAEFLKSAKQSFAGIIIGNQIRTDQ 180

Query: 186 KFMGVFEESLKERQEAEKKGELAGGDWFDIFLSFVFGKEQSSDLKETLNQEPRPSVEQNI 245
KFMGVF+ESLKERQEAEK GE GGDW DIFLSF+F K+QSSD+KE +NQEP P V+ +I
Sbjct: 181 KFMGVFDESLKERQEAEKNGEPTGGDWLDIFLSFIFDKKQSSDVKEAINQEPVPHVQPDI 240

Query: 246 ATTPTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKFNQLLIHNNALS 305
ATT TDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKFNQLLIHNNALS
Sbjct: 241 ATTTTDIQGLPPEARDLLDERGNFSKFTLGDMEMLDVEGVADIDPNYKFNQLLIHNNALS 300

Query: 306 SMLMGSHSNIEPEKVSLLYGDNGGPEARHDWNATVGYKNQQGNNVATLINMHLKNGSGLV 365
S+LMGSH+ IEPEKVSLLYG NGGP ARHDWNATVGYK+QQGNNVAT+IN+H+KNGSGLV
Sbjct: 301 SVLMGSHNGIEPEKVSLLYGGNGGPGARHDWNATVGYKDQQGNNVATIINVHMKNGSGLV 360

Query: 366 IAGNENGINNPSFYLYKKDQLTGLKQALSQEEIQNKVDFMEFLAQNNARLDNLSEEEKGK 425
IAG E GINNPSFYLYK+DQLTG ++ALSQEEIQNK+DFMEFLAQNNA+LDNLSE+EK K
Sbjct: 361 IAGGEKGINNPSFYLYKEDQLTGSQRALSQEEIQNKIDFMEFLAQNNAKLDNLSEKEKEK 420

Query: 426 FQNEIGNFQKDPKAYLDALGNDRIAFVSKKDQKHLALVTEFGNGGLSYTLKDYGKKQDRA 485
F+ EI +FQKD KAYLDALGNDRIAFVSKKD KH AL+TEFGNG LSYTLKDYGKK D+A
Sbjct: 421 FRTEIKDFQKDSKAYLDALGNDRIAFVSKKDTKHSALITEFGNGDLSYTLKDYGKKADKA 480

Query: 486 LDRETKTTLQGNLKDDGVMFVNYSNFKYTNASKSPNEGIGATNGVSHLEANFSKVAVFNL 545
LDRE TLQG+LK DGVMFV+YSNFKYTNASK+PN+G+G TNGVSHLE F+KVA+FNL
Sbjct: 481 LDREKNVTLQGSLKHDGVMFVDYSNFKYTNASKNPNKGVGVTNGVSHLEVGFNKVAIFNL 540

Query: 546 SDLNSLAISSFARRNLEDTLIAKGLPPKEANKIIKDFLNSNKELLGKSLNFNKAVAEAKN 605
DLN+LAI+SF RRNLED L KGL P+EANK+IKDFL+SNKEL+GK+LNFNKAVA+AKN
Sbjct: 541 PDLNNLAITSFVRRNLEDKLTTKGLSPQEANKLIKDFLSSNKELVGKTLNFNKAVADAKN 600

Query: 606 TGNYDGVKKAQKDLEKSIRKRELLEKEVTKKFESKSGNKNKMEAKAQANSKKDEIFALIN 665
TGNYD VKKAQKDLEKS+RKRE LEKEV KK ESKSGNKNKMEAKAQANS+KDEIFALIN
Sbjct: 601 TGNYDEVKKAQKDLEKSLRKREHLEKEVEKKLESKSGNKNKMEAKAQANSQKDEIFALIN 660

Query: 666 KEAYKDARITAYAQNLKNIKRELSDKIENINKNLKDFGQSFDALKSGKNKDFSKAEETLK 725
KEA +DAR AYAQNLK IKRELSDK+EN+NKNLKDF +SFD K+GKNKDFSKAEETLK
Sbjct: 661 KEANRDARAIAYAQNLKGIKRELSDKLENVNKNLKDFDKSFDEFKNGKNKDFSKAEETLK 720

Query: 726 ALKSSVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSDLENSIKDVLINQEI 785
ALK SVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSDLENS+KDV+INQ++
Sbjct: 721 ALKGSVKDLGINPEWISKVENLNAALNEFKNGKNKDFSKVTQAKSDLENSVKDVIINQKV 780

Query: 786 TDKVDNLNQAVLIAKAIGDFSGVEQALAELKNFSTDQ-----------KVGKNSDLQKSV 834
TDKVDNLNQAV +AKA GDFS VEQALA+LKNFS +Q K S++ +SV
Sbjct: 781 TDKVDNLNQAVSVAKATGDFSRVEQALADLKNFSKEQLAQQAQKNESLNARKKSEIYQSV 840

Query: 835 KNGVNEILVGNGLSKTEATTLTKKFSDIKKELNEKFANFNKNNDGLKNSAEPIYAQVNKK 894
KNGVN LVGNGLS+ EATTL+K FSDIKKELN K NFN NN+ EPIYA+VNKK
Sbjct: 841 KNGVNGTLVGNGLSQAEATTLSKNFSDIKKELNAKLGNFN-NNNNNGLKNEPIYAKVNKK 899

Query: 895 KTGQAASPEEPIYTQVAKKVNAKIDQLNKNASASKGVGDFSGAGRLDSPEPIYATIDDLG 954
K GQAAS EEPIY QVAKKVNAKID+LN+ AS VG +G
Sbjct: 900 KAGQAASLEEPIYAQVAKKVNAKIDRLNQIASGLGVVGQAAG------------------ 941

Query: 955 GSFSLKRHAKVDDLTKVGLSREQELTQKIGNLNQAVSEAKAGFFGNLEQTMDRLKDSTKK 1014
F LKRH KVDDL+KVGLSR QEL QKI NLNQAVSEAKAGFFGNLEQT+D+LKDSTK
Sbjct: 942 --FPLKRHDKVDDLSKVGLSRNQELAQKIDNLNQAVSEAKAGFFGNLEQTIDKLKDSTKH 999

Query: 1015 NVVNLWVEDTKKVPTSLQAKLDNYTTNNHTRINSNVKNGTINEKAIGMLTQKNPEWLKLV 1074
N +NLWVE KKVP SL AKLDNY TN+H RINSN+KNG INEKA GMLTQKNPEWLKLV
Sbjct: 1000 NPMNLWVESAKKVPASLSAKLDNYATNSHIRINSNIKNGAINEKATGMLTQKNPEWLKLV 1059

Query: 1075 NDKIVAHNVGSTPLSDYDKIGFNQKNMKDYSDSFKFSTKLSNAVKNIKSGFEQFLTDCIS 1134
NDKIVAHNVGS PLS+YDKIGFNQKNMKDYSDSFKFSTKL+NAVK+ SGF QFLT+ S
Sbjct: 1060 NDKIVAHNVGSVPLSEYDKIGFNQKNMKDYSDSFKFSTKLNNAVKDTNSGFTQFLTNAFS 1119

Query: 1135 AGSYNPM---KAEHGVKNINTKSGFQKS 1159
SY + AEHG+KN+NTK GFQKS
Sbjct: 1120 TASYYCLARENAEHGIKNVNTKGGFQKS 1147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04125PF043351186e-35 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 118 bits (298), Expect = 6e-35
Identities = 44/205 (21%), Positives = 74/205 (36%), Gaps = 10/205 (4%)

Query: 27 KLNKANRTFKRAFYL---SMALNIAAVTSIVMMMPLKKTDIFVYGIDRYTGEFKIVKRSD 83
KL A R+ K A+ + + AL A V ++ + PLK + +V +DR TGE I +
Sbjct: 24 KLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLH 83

Query: 84 A-RQIVNSEAVVDSATSKFVSLLFGYSKNSLRDRKDQLMQYCDVSFQTQAMRMFNENIRQ 142
I EAV + +V G+ + + D +M Q + R + + Q
Sbjct: 84 GDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDNPQ 143

Query: 143 FVDKVRA-EAIISSNIRREKVKNSPLTRLTFFITIKITPDTMENYEYITKKEVTIYYDFA 201
+ A + I + +F +T T TI Y
Sbjct: 144 SPQNILANRTDVFVEI-KRVSFLGGNVAQVYFTKESVTGSNS----TKTDAVATIKYKVD 198

Query: 202 RGNSSQENLIINPFGFKVFDIQITD 226
S + + NP G++V +
Sbjct: 199 GTPSKEVDRFKNPLGYQVESYRADV 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04135TYPE4SSCAGX8690.0 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 869 bits (2246), Expect = 0.0
Identities = 511/522 (97%), Positives = 514/522 (98%)

Query: 1 MEQAFFKKIVGCFCLGYLFLSSVIEAAAPDIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 60
M QAFFKKIVGCFCLGYLFLSS IEA A DIKNFNRGRVKVVNKKIAYLGDEKPITIWTS
Sbjct: 1 MGQAFFKKIVGCFCLGYLFLSSAIEAVALDIKNFNRGRVKVVNKKIAYLGDEKPITIWTS 60

Query: 61 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 120
LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR
Sbjct: 61 LDNVTVIQLEKDETISYITTGFNKGWSIVPNSNHIFIQPKSVKSNLMFEKEAVNFALMTR 120

Query: 121 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANL 180
DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANL
Sbjct: 121 DYQEFLKTKKLIVDAPDPKELEEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANL 180

Query: 181 ENLTNAMSNPQNLSNNKNLSEFIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQA 240
ENLTNAMSNPQNLSNNKNLSE IKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQA
Sbjct: 181 ENLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQA 240

Query: 241 EETIKQRAKDKINIKTDKPQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKD 300
EE ++QRAKDKI+IKTDK QKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKD
Sbjct: 241 EEAVRQRAKDKISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKD 300

Query: 301 NFASAYLTVKLEYPQRHEVSSVIEGELKKREEAKRQRELIKQENLNTTAYINRVMMASNE 360
NFASAYLTVKLEYPQRHEVSSVIE ELKKREEAKRQRELIKQENLNTTAYINRVMMASNE
Sbjct: 301 NFASAYLTVKLEYPQRHEVSSVIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNE 360

Query: 361 QIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIF 420
QIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIF
Sbjct: 361 QIINKEKIREEKQKIILDQAKALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIF 420

Query: 421 DDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIK 480
DDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIK
Sbjct: 421 DDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIK 480

Query: 481 DKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 522
DKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK
Sbjct: 481 DKALVTVINKGYGKNPLTKNYNIKNYGELERVIKKLPLVRDK 522


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04140IGASERPTASE392e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 38.9 bits (90), Expect = 2e-04
Identities = 30/159 (18%), Positives = 63/159 (39%), Gaps = 21/159 (13%)

Query: 581 TPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDC 640
P ++ + + ++KT + ++ T + ++ +EAK +VKA
Sbjct: 1023 APVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQT 1082

Query: 641 VSQAKTEAEKKECEKLLTPEARKLLEQQALDCLKNAKTEAEKKRCVKDLPKDLQKKVLAK 700
A++ +E KE + T E + +++ AK E EK + V KV ++
Sbjct: 1083 NEVAQSGSETKETQTTETKETATVEKEE------KAKVETEKTQEV--------PKVTSQ 1128

Query: 701 ESVKAYLDCVSQAKTEAEKKECEKLLTPEAKKLLEEAKE 739
S K Q ++E + + E + ++E +
Sbjct: 1129 VSPK-------QEQSETVQPQAEPARENDPTVNIKEPQS 1160



Score = 36.6 bits (84), Expect = 0.001
Identities = 34/217 (15%), Positives = 69/217 (31%), Gaps = 14/217 (6%)

Query: 872 TPEAKKLLEQEVKKSVKAYLDCVSQAKTEAEKKECEKLLTPEARKFLAKQALDCLKNAKT 931
TP E K ++ Q TE + E ++ Q + ++
Sbjct: 1032 TPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE 1091

Query: 932 EAEKKRCVKDLPKDLQKKVLAKESVKAYLDCVSRARNEKEKKECEKLLTPEARKLLEEAK 991
E + ++K+ AK + + K+E + + P+A E A+
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQA----EPAR 1147

Query: 992 ESLKAYKDCLSQARNEEERKACEKLLTPEARKLLEQEVKKSVKAYLDCVSQAKTEAEKKE 1051
E+ + + +++ A + E +EQ V +S + T E
Sbjct: 1148 ENDPTVN--IKEPQSQTNTTADTEQPAKETSSNVEQPVTES--------TTVNTGNSVVE 1197

Query: 1052 CEKLLTPEARKFLAKQALSCLEKARNEEERKACLKNI 1088
+ TP + S K R+ ++ N+
Sbjct: 1198 NPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNV 1234



Score = 35.0 bits (80), Expect = 0.003
Identities = 39/245 (15%), Positives = 91/245 (37%), Gaps = 12/245 (4%)

Query: 674 KNAKTEAEKKRCVKDLP-KDLQKKVLAKESVKAYLDCVSQAKTEAEKKECEKLLTPEAKK 732
+ + E+ V + P ++ + + ++KT + ++ T + ++
Sbjct: 1008 PSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNRE 1067

Query: 733 LLEEAKESLKAYKDCVSRARNEKEKKECEKLLTPEAKKLLEQQALDCLKNAKTEAEKKRC 792
+ +EAK ++KA A++ E KE + T E + +++ KT+ K
Sbjct: 1068 VAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVET-EKTQEVPKVT 1126

Query: 793 VKDLPKDLQKKVL------AKESVKAYL--DCVSQAKTEAEKKECEKLLTPEARKLLEEA 844
+ PK Q + + A+E+ + SQ T A+ ++ K + + + E+
Sbjct: 1127 SQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTES 1186

Query: 845 KESLKAYKDCVSRARNEKEKKECEKLLTPEAKKLLEQEVKKSVKAYLDCVSQAKTEAEKK 904
+ V + + E+ + ++SV++ V A T + +
Sbjct: 1187 TTVNTG--NSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDR 1244

Query: 905 ECEKL 909
L
Sbjct: 1245 STVAL 1249



Score = 33.9 bits (77), Expect = 0.008
Identities = 28/187 (14%), Positives = 67/187 (35%), Gaps = 4/187 (2%)

Query: 497 KARNEKEKKECEKLLTPEAKKKLEQQVLDCLKNAKTDEERKKCLKDLPKD--LQSDILAK 554
+ NE+ + E + P A + +N+K + + + + + Q+ +AK
Sbjct: 1011 PSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAK 1070

Query: 555 ESVKAYKDCVSQARTEAEKKECEKLLTPEAKKLLEEEAKESVKAYLDCVSQAKTEAEKKE 614
E+ K E ++ T E K+ E +E K + + +
Sbjct: 1071 EAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVS 1130

Query: 615 CEKLLTPEAKKKLEEAKKSVKAYL--DCVSQAKTEAEKKECEKLLTPEARKLLEQQALDC 672
++ + + + E A+++ + SQ T A+ ++ K + + + +
Sbjct: 1131 PKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVN 1190

Query: 673 LKNAKTE 679
N+ E
Sbjct: 1191 TGNSVVE 1197



Score = 32.3 bits (73), Expect = 0.019
Identities = 38/242 (15%), Positives = 88/242 (36%), Gaps = 6/242 (2%)

Query: 528 KNAKTDEERKKCLKDLPKDLQSDILAKESVKAYKDCVSQARTEAEKKECEKLLTPEAKKL 587
+ ++ E + + P + E+ + + Q EK E + T +
Sbjct: 1008 PSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNRE 1067

Query: 588 LEEEAKESVKAYLDC--VSQAKTEAEKKECEKLLTPEAKKKLEEAKKSVKAYLDCVSQAK 645
+ +EAK +VKA V+Q+ +E ++ + + +K E+AK + +
Sbjct: 1068 VAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTS 1127

Query: 646 TEAEKKECEKLLTPEARKLLEQQALDCLKNAK----TEAEKKRCVKDLPKDLQKKVLAKE 701
+ K+E + + P+A E +K + T A+ ++ K+ ++++ V
Sbjct: 1128 QVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTEST 1187

Query: 702 SVKAYLDCVSQAKTEAEKKECEKLLTPEAKKLLEEAKESLKAYKDCVSRARNEKEKKECE 761
+V V + + + + K + S+++ V A +
Sbjct: 1188 TVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTV 1247

Query: 762 KL 763
L
Sbjct: 1248 AL 1249


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04170PF07201290.035 Hypersensitivity response secretion protein HrpJ
		>PF07201#Hypersensitivity response secretion protein HrpJ

Length = 293

Score = 29.4 bits (66), Expect = 0.035
Identities = 11/53 (20%), Positives = 21/53 (39%), Gaps = 10/53 (18%)

Query: 328 EEREKKLLAEKEKQ--------EAELAKY--KLKDLENQKKLKALEAELKKKN 370
E +E L K E ++ +Y K+ +LE ++ + L + L
Sbjct: 62 ERKELSLDKRKLSDSQARVSDVEEQVNQYLSKVPELEQKQNVSELLSLLSNSP 114


10HPSAT_04300HPSAT_04325Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_04300516-1.701496hypothetical protein
HPSAT_04305718-1.266458hypothetical protein
HPSAT_04310615-1.05151150S ribosomal protein L28
HPSAT_04315413-1.556229putative potassium channel protein
HPSAT_0432059-1.910314hypothetical protein
HPSAT_0432539-1.682688hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04300PF052112799e-97 Neuraminyllactose-binding hemagglutinin
		>PF05211#Neuraminyllactose-binding hemagglutinin

Length = 260

Score = 279 bits (714), Expect = 9e-97
Identities = 67/285 (23%), Positives = 126/285 (44%), Gaps = 43/285 (15%)

Query: 1 MERSLIFKKVRVYSKMLVVLGLSSVLIGCAMNPSAETKTQNDAKNQQPVQTHERMKTSSE 60
M+ + FK + K L+ + ++L+GC S N+ + H +SE
Sbjct: 1 MKANNHFKDF-AWKKCLLGASVVALLVGC----SPHIIETNEVAL--KLNYH----PASE 49

Query: 61 YVTPLDFNYAIHIVQAPQNHHIVGILAPRIQVSDNL-KPYIDKFQDALANQIQTIFEKRG 119
V LD + +L P Q SDN+ K Y +KF++ +++ I + +G
Sbjct: 50 KVQALD--------------EKILLLRPAFQYSDNIAKEYENKFKNQTTLKVEQILQNQG 95

Query: 120 YQVLRF--QDEKALSVQDKRKIFSVLDLKGWVGILEDLKMNLKDPNNPNL--NTLVDQ-- 173
Y+V+ D+ S K++ + + + G + + D K ++ + P L +T +D+
Sbjct: 96 YKVINVDSSDKDDFSFAQKKEGYLAVAMNGEIVLRPDPKRTIQKKSEPGLLFSTGLDKME 155

Query: 174 ----SSGSVWFNFYEPESNRVVHDFAVEVGTF---QAMTYTYKQSNSGGLNSSNSIIHED 226
+G V EP S + F +++ + T S+SGGL S+
Sbjct: 156 GVLIPAGFVKVTILEPMSGESLDSFTMDLSELDIQEKFLKTTHSSHSGGLVSTMV----K 211

Query: 227 LEKNKEDAIHKILNRMYAVVMKKAVTELTEENIAKYREAIDRMKG 271
N DAI LN+++A +M++ +LT++N+ Y++ +KG
Sbjct: 212 GTDNSNDAIKSALNKIFANIMQEIDKKLTQKNLESYQKDAKELKG 256


11HPSAT_04700HPSAT_04725Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_047002141.010117hypothetical protein
HPSAT_047053141.105249glycyl-tRNA synthetase subunit alpha
HPSAT_047104151.013172NAD(P)H-dependent glycerol-3-phosphate
HPSAT_04715615-0.028469hypothetical protein
HPSAT_04720414-0.017795putative ATPase
HPSAT_04725312-0.196250hypothetical protein
12HPSAT_04805HPSAT_04850Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_04805314-0.021437cell division protein FtsA
HPSAT_04810318-1.427188cell division protein FtsZ
HPSAT_04825122-4.216047mechanosensitive channel MscS
HPSAT_04830428-5.214062hypothetical protein
HPSAT_04835429-4.945205exodeoxyribonuclease VII large subunit
HPSAT_04840630-5.409137hypothetical protein
HPSAT_04845830-5.715135hypothetical protein
HPSAT_04850421-4.975319hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04805SHAPEPROTEIN418e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 40.9 bits (96), Expect = 8e-06
Identities = 39/181 (21%), Positives = 70/181 (38%), Gaps = 13/181 (7%)

Query: 211 AASIATLSNDERELGVACVDIGGETCNLTIYSGNSIRYNKYLPIGSHHLSTDLSSMLNTP 270
AA+I G VDIGG T + + S N + Y+ + IG + + +
Sbjct: 146 AAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRN 205

Query: 271 F------PYAEEVKIKYGDLSFESGEETPSQSVQIPTTGSDGNESHVVPLSKIQNIMRDR 324
+ AE +K + G S G+E V+ + ++I +++
Sbjct: 206 YGSLIGEATAERIKHEIG--SAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEP 263

Query: 325 ALETFQIIHRSIQDSGLE---EHLGGGVVLTGGMALMKGIKELAKAHFTNYPVRLAA-PM 380
+ +++ E + G+VLTGG AL++ + L T PV +A P+
Sbjct: 264 LTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLL-MEETGIPVVVAEDPL 322

Query: 381 E 381

Sbjct: 323 T 323


13HPSAT_05270HPSAT_05295Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_05270210-0.788150glucokinase
HPSAT_05275411-1.962604NADP-dependent alcohol dehydrogenase
HPSAT_05280212-2.222870putative lipopolysaccharide biosynthesis
HPSAT_05285313-1.137610putative lipopolysaccharide biosynthesis
HPSAT_052902131.613250hypothetical protein
HPSAT_052952122.484856hypothetical protein
14HPSAT_05370HPSAT_05410Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_05370318-1.592995type II DNA modification enzyme
HPSAT_05375520-0.998105hypothetical protein
HPSAT_05380314-1.265205flgM protein
HPSAT_05385413-2.098956hypothetical protein
HPSAT_05390413-1.932293FKBP-type peptidyl-prolyl cis-trans isomerase
HPSAT_05395414-2.630451hypothetical protein
HPSAT_05400514-2.387574peptidoglycan-associated lipoprotein precursor
HPSAT_05405214-0.251324translocation protein TolB
HPSAT_05410217-0.263873hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_05395GPOSANCHOR330.001 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 33.1 bits (75), Expect = 0.001
Identities = 25/145 (17%), Positives = 48/145 (33%)

Query: 27 GATKKELKQLQVNSKNFSNILTKIHSQVEANTQAQEGLRSVYEGQANKIKDLNNAILSQE 86
K + ++ +A EG + + KIK L + E
Sbjct: 200 EGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALE 259

Query: 87 ESLRALKALQEVQANTLKQQSQTLEDLRNEIHANQQAIQQLDKQNKEMSELLTKLSQDLV 146
L+ E N S ++ L E A + L+ Q++ ++ L +DL
Sbjct: 260 ARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLD 319

Query: 147 SQIALIQKALKEQEEKAEKPLKSNA 171
+ ++ E ++ E+ S A
Sbjct: 320 ASREAKKQLEAEHQKLEEQNKISEA 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_05400OMPADOMAIN1411e-43 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 141 bits (358), Expect = 1e-43
Identities = 43/168 (25%), Positives = 69/168 (41%), Gaps = 22/168 (13%)

Query: 22 NMNKETVAGDVSAKAVQSAPVSTETVQEKQEPKQEPAPVVEKKPAIESGTIIASIYFDFD 81
+ ++ VS + Q P PAP V+ K T+ + + F+F+
Sbjct: 177 RPDNGMLSLGVSYRFGQGEAAPVV------APAPAPAPEVQTK----HFTLKSDVLFNFN 226

Query: 82 KYEIKESDQETLDEIVQKAKE---NHMQVLLEGNTDEFGSSEYNQALGVKRTLSVKNALI 138
K +K Q LD++ + V++ G TD GS YNQ L +R SV + LI
Sbjct: 227 KATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLI 286

Query: 139 IKGVEKDMIKTISFGETKPKCTQKT---------KECYKENRRVDVKL 177
KG+ D I GE+ P +C +RRV++++
Sbjct: 287 SKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_05410TYPE4SSCAGA330.002 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 32.8 bits (74), Expect = 0.002
Identities = 38/142 (26%), Positives = 67/142 (47%), Gaps = 11/142 (7%)

Query: 32 EEAEKILLDLGKKNEQVID--LNLEDLPSDEKKEEKVEE-KKEEKVVEKNATDKEGDFID 88
+EA K++ D N++++ LN +D K +E KK +K +EK+ +E
Sbjct: 568 QEANKLIKDFLSSNKELVGKTLNFNKAVADAKNTGNYDEVKKAQKDLEKSLRKREHL--- 624

Query: 89 PKEQEESLENIFSSLNDFQEKTDTNAQKDE----QKNEQEEEQRRLKEQQRLRKNQKN-Q 143
KE E+ LE+ + N + K N+QKDE E + R + Q L+ ++
Sbjct: 625 EKEVEKKLESKSGNKNKMEAKAQANSQKDEIFALINKEANRDARAIAYAQNLKGIKRELS 684

Query: 144 EMLKGLQQNLDQFAQKLESVKN 165
+ L+ + +NL F + + KN
Sbjct: 685 DKLENVNKNLKDFDKSFDEFKN 706


15HPSAT_05925HPSAT_06020Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_05925212-0.694700hypothetical protein
HPSAT_05930312-1.201451DNA polymerase III subunit delta'
HPSAT_05935012-1.031115dihydropteroate synthase
HPSAT_05940114-0.120405hypothetical protein
HPSAT_05945-1100.730985hypothetical protein
HPSAT_05950-1100.725783integral membrane protein
HPSAT_05955-1141.689174hypothetical protein
HPSAT_05960-2102.514280hypothetical protein
HPSAT_05965-1102.794648carbamoyl phosphate synthase small subunit
HPSAT_05970093.300746formamidase
HPSAT_05975-1102.876323hypothetical protein
HPSAT_05980-1103.017529Maf-like protein
HPSAT_059850112.796049alanyl-tRNA synthetase
HPSAT_059900131.232984hypothetical protein
HPSAT_05995-2100.603986outer membrane protein (omp19)
HPSAT_06000211-1.05365430S ribosomal protein S18
HPSAT_06005212-1.038568single-stranded DNA-binding protein
HPSAT_06010211-1.00274230S ribosomal protein S6
HPSAT_06015311-0.685900DNA polymerase III subunit delta
HPSAT_06020212-0.583904ribonuclease R
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_05990PF05844250.035 YopD protein
		>PF05844#YopD protein

Length = 295

Score = 25.0 bits (54), Expect = 0.035
Identities = 13/65 (20%), Positives = 28/65 (43%), Gaps = 1/65 (1%)

Query: 10 SVLKANNPHFDKIFEKHNQLDDDIKTAEQQNASDAEVSHMKKQKLKLKDEIHSMIIEYRE 69
L+A F+ + I++ Q + +V + Q ++E+++ I + +
Sbjct: 197 VALRAAGRAFESRNGALQVANTVIQSFVQMANASVQVRQGESQASAREEEVNATIGQ-SQ 255

Query: 70 KQKSE 74
KQK E
Sbjct: 256 KQKVE 260


16HPSAT_06605HPSAT_06630Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_06605013-4.115911hypothetical protein
HPSAT_06610013-5.912371signal-transducing protein, histidine kinase
HPSAT_06615-115-6.361187putative transcriptional regulator
HPSAT_06620-113-6.082081type IIS restriction enzyme R protein (MBOIIR)
HPSAT_06625-112-5.281713type II restriction endonuclease
HPSAT_06630-111-3.291515DNA adenine methylase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_06615HTHFIS912e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 90.7 bits (225), Expect = 2e-23
Identities = 37/118 (31%), Positives = 57/118 (48%), Gaps = 2/118 (1%)

Query: 1 MQK-KIFLLEDDYLLSESVKEFLEHLGYEVFCAFNGKEAYERLSVERFNLLLLDVQVPEM 59
M I + +DD + + + L GY+V N + ++ +L++ DV +P+
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 NSLELFRRIKNDFLIFTPVIFITALHDNATLKNAFNLGASDYLKKPFDLDELEARIKR 117
N+ +L RIK PV+ ++A + T A GA DYL KPFDL EL I R
Sbjct: 61 NAFDLLPRIKKA-RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_06625PF00577300.010 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 30.2 bits (68), Expect = 0.010
Identities = 7/45 (15%), Positives = 16/45 (35%)

Query: 122 GSHGEYFRNRASTKNIQFPYSHYLAHICLGILYTRSASSGIDETE 166
GSH Y+ + Q + I + Y+ + ++ +
Sbjct: 547 GSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD 591


17HPSAT_07055HPSAT_07135Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_07055210-0.319918outer membrane protein
HPSAT_07060211-0.160250branched-chain amino acid aminotransferase
HPSAT_07065212-0.918274outer membrane protein (omp31)
HPSAT_07070213-1.132902DNA polymerase I (polA)
HPSAT_07075118-0.295287type II restriction enzyme
HPSAT_07080219-0.045090type II restriction modification enzyme
HPSAT_070853211.514958hypothetical protein
HPSAT_070902150.847287thymidylate kinase
HPSAT_070953130.367524phosphopantetheine adenylyltransferase
HPSAT_071003130.4866063-octaprenyl-4-hydroxybenzoate carboxy-lyase
HPSAT_071053120.227750hypothetical protein
HPSAT_071103130.289228flagellar basal body P-ring biosynthesis protein
HPSAT_071152110.280990DNA helicase II
HPSAT_071201130.380706hypothetical protein
HPSAT_071252130.913595seryl-tRNA synthetase
HPSAT_071301160.295291hypothetical protein
HPSAT_07135215-0.036901exodeoxyribonuclease VII small subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07095LPSBIOSNTHSS2212e-77 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 221 bits (565), Expect = 2e-77
Identities = 64/148 (43%), Positives = 94/148 (63%)

Query: 4 IGIYPGTFDPVTNGHIDIIHRSSELFEKLIVAVAYSCAKNPMFSLKERLEMMQLATKGFK 63
IYPG+FDP+T GH+DII R LF+++ VAV + K PMFS++ERLE + A
Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61

Query: 64 NVECVAFEGLLANLAKEYHCKVLVRGLRVVSDFEYELQMGYANKSLNHELETLYFMPTLQ 123
N + +FEGL N A++ ++RGLRV+SDFE ELQM NK+L +LET++ + +
Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121

Query: 124 NAFISSSIVRSIIAHKGDASHLVPKEIH 151
+F+SSS+V+ + G+ H VP +
Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHVA 149


18HPSAT_07185HPSAT_07225Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_07185212-1.967427conserved hypothetical nifU-like protein
HPSAT_07190111-1.917490hypothetical protein
HPSAT_07195110-3.062064UDP-N-acetylmuramoylalanyl-D-glutamate--2,
HPSAT_07200111-4.662900transaldolase
HPSAT_07205014-6.11541150S ribosomal protein L25/general stress protein
HPSAT_07210114-4.819039peptidyl-tRNA hydrolase
HPSAT_07215115-5.191922hypothetical protein
HPSAT_07220318-5.683716type II methylase
HPSAT_07225112-3.194799DNA adenine methylase
19HPSAT_07370HPSAT_07515Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_07370-113-3.633133FAD-dependent thymidylate synthase
HPSAT_07375-211-1.587808type I R-M system specificity subunit
HPSAT_07380-211-1.173331hypothetical protein
HPSAT_07385-211-0.633822type I restriction enzyme M protein (hsdM)
HPSAT_07390-1110.664544type I restriction enzyme R protein (hsdR)
HPSAT_073952122.956110hypothetical protein
HPSAT_074002113.304474iron(III) dicitrate transport protein (fecA)
HPSAT_074050100.862530hypothetical protein
HPSAT_0741009-0.499119arginase
HPSAT_07415010-0.452840alanine dehydrogenase
HPSAT_07430210-2.242747putative outer membrane protein
HPSAT_07435411-3.104147probable inorganic polyphosphate/ATP-NAD kinase
HPSAT_07440514-4.277347DNA repair protein RecN
HPSAT_07445214-4.633692fibronectin/fibrinogen-binding protein
HPSAT_07450316-1.494454hypothetical protein
HPSAT_07455019-1.720514hypothetical protein
HPSAT_07460017-1.128490hypothetical protein
HPSAT_07465-114-1.533823hypothetical protein
HPSAT_07470012-1.700697DNA polymerase III subunit epsilon
HPSAT_07475013-3.144436ribulose-phosphate 3-epimerase
HPSAT_07480014-4.435843fructose-1,6-bisphosphatase
HPSAT_07485017-5.376675hypothetical protein
HPSAT_07490218-6.685657putative type II methylase protein
HPSAT_07495722-7.509808hypothetical protein
HPSAT_07500624-7.863323hypothetical protein
HPSAT_07505318-4.148941hypothetical protein
HPSAT_07510317-3.308423hypothetical protein
HPSAT_07515211-2.332903hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07385TYPE4SSCAGA320.008 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 32.4 bits (73), Expect = 0.008
Identities = 72/270 (26%), Positives = 117/270 (43%), Gaps = 33/270 (12%)

Query: 545 HLEPGFNPKTL--IESVCSKVLKEFEKVEILDKYGVYQLFKDYYNEVLQDDWFLLSFNDF 602
HLE GFN + + + + + F + + DK L N++++D LS N
Sbjct: 527 HLEVGFNKVAIFNLPDLNNLAITSFVRRNLEDKLTTKGLSPQEANKLIKD---FLSSNKE 583

Query: 603 LSTKELRELNPLKDKNKKANYLEEPDFVIQKTYYKSDLIPKNLIKQRFFEKE-AKELEQL 661
L K L + D NY E ++K + DL K+L K+ EKE K+LE
Sbjct: 584 LVGKTLNFNKAVADAKNTGNYDE-----VKKA--QKDL-EKSLRKREHLEKEVEKKLESK 635

Query: 662 ENALNEKEADFEEFIEEHSGEEGLFYELKINESVLKKELKNATDLEDKEILKTALELLEA 721
N+ EA + +S ++ +F IN+ + A K I + + LE
Sbjct: 636 SGNKNKMEAK----AQANSQKDEIF--ALINKEANRDARAIAYAQNLKGIKRELSDKLEN 689

Query: 722 KNKALKMKNKAHEELE-------LKAFHQYKNLKLGEIKDLIIQDKWLKSLKNALENKIL 774
NK LK +K+ +E + KA K LK G +KDL I +W+ ++N +
Sbjct: 690 VNKNLKDFDKSFDEFKNGKNKDFSKAEETLKALK-GSVKDLGINPEWISKVEN-----LN 743

Query: 775 KRINAFTSALNKIISNYSNSLLELDKEVKE 804
+N F + NK S + + +L+ VK+
Sbjct: 744 AALNEFKNGKNKDFSKVTQAKSDLENSVKD 773


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07445FbpA_PF058331119e-29 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 111 bits (280), Expect = 9e-29
Identities = 77/401 (19%), Positives = 152/401 (37%), Gaps = 29/401 (7%)

Query: 54 KKPPESVLKNTLALDFCLNKFTKNAKILQANIIDNDRILEITGAKDLAYKSENFILRLEM 113
K P + + N N I + L + ++ ++ +N + L +
Sbjct: 170 KLNPFDFSYDMIENFTKENSLQLNDNIFSKIFTGVSKTL----SSEICFRLKNNSIDLSL 225

Query: 114 IPKKANLMILDKEKCVIEA--FRFNDRVAKNDILGALPLN-TYEHQERDLDFKGLLDILE 170
K + + I++ F FN N +G LN + + + + +LE
Sbjct: 226 SNLKEIVEVCKDLFKEIQSNKFEFNCYTKNNSFVGFYCLNLMSKEDYKKIQYDSSSKLLE 285

Query: 171 KDFLSYQHKE-LERKKNHIIKRLNMQKERLKEKLEKLEDPKNLQLEAKELQTQASLLLTY 229
+ + + L+ K + + K + R +K + L + + + LL
Sbjct: 286 NFYYAKDKSDRLKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCEDKDIFKLYGELLTAN 345

Query: 230 QHLINKHESRVVLKDFED---KECAIEIDKSMPLNAFINKKFTLSKKKKQKSQFLYLEEE 286
+ + K S + L ++ I +D++ + + + K K+ + +
Sbjct: 346 IYALKKGLSHIELANYYSENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAANEQLL 405

Query: 287 NLKEKIAFKENQINYVKGAQEESVLE------------MFMPVKNSKIKRPMSGYEVLYY 334
+E++ + + + + A +E F + SK + +
Sbjct: 406 QNEEELNYLYSVLTNINNADNYDEIEEIKKELIETGYIKFKKIYKSKKSKTSKPMHFISK 465

Query: 335 KDFKIGLGKNQKENIKL-LQDARANDLWMHVRDIPGSHLIVFCQKNTPKDEIIMELAKML 393
I +GKN +N L L+ A +D+W H ++IPGSH+IV + P + ++E A +
Sbjct: 466 DGIDIYVGKNNIQNDYLTLKFANKHDIWFHTKNIPGSHVIVKNIMDIP-ESTLLEAANLA 524

Query: 394 IKMQKDAFNS-YEIDYTQRKFVKIIKGAN---VIYSKYRTI 430
K +S +DYT+ K VK GA VIYS +TI
Sbjct: 525 AYYSKSQNSSNVPVDYTEVKNVKKPNGAKPGMVIYSTNQTI 565



Score = 34.8 bits (80), Expect = 6e-04
Identities = 21/92 (22%), Positives = 48/92 (52%), Gaps = 5/92 (5%)

Query: 46 NAPYIGLSKKPPESVLKNTLALDFCLNKFTKNAKILQANIIDNDRI--LEITGAKDLAYK 103
N P I L+ + +K + L K+ NAKI+ + I+ DRI ++ +L +
Sbjct: 55 NYPRIHLTDLTKPNPIKAPMFCMV-LRKYISNAKIVDIHQINQDRIVVIDFESTDELGFN 113

Query: 104 SENFILRLEMIPKKANLMILDK-EKCVIEAFR 134
S + L +E++ + +N+ ++ K + ++++ +
Sbjct: 114 SI-YSLIIEIMGRHSNMTLIRKRDNIIMDSIK 144


20HPSAT_00195HPSAT_00220N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_00195-2130.418859competence protein
HPSAT_00200-2120.400780comB9 competence protein
HPSAT_00205-2121.174097comB10 competence protein
HPSAT_00210-1111.127123mannose-6-phosphate isomerase
HPSAT_00215-1121.198829GDP-D-mannose dehydratase
HPSAT_002200131.050568nodulation protein (nolK)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00195PF043351318e-40 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 131 bits (331), Expect = 8e-40
Identities = 39/203 (19%), Positives = 75/203 (36%), Gaps = 6/203 (2%)

Query: 40 QSVFRLERNRLKIAYKLLGLMSFIALVLAIVLISVLPLQKTEHHF--VDFLNQDKHYAII 97
+ K+A+ + G+ +A + + ++ PL+ E + VD + A
Sbjct: 22 RDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAK 81

Query: 98 QRADKSISSNEALARSLIGAYVLNRESINRIDDKSRYELVRLQSSSKVWQRFEDLIKTQN 157
D +I+ +EA+ + + YV RE + ++ V + S+ R+ KT N
Sbjct: 82 LHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDN 141

Query: 158 SIYAQSHLEREVHI-VNIAIYQQDNNPIASVSIAAKLMNENKLVYEKRYKIA-LSYLFDT 215
Q+ L + V I +A V + + + K +A + Y D
Sbjct: 142 PQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNST--KTDAVATIKYKVDG 199

Query: 216 PDFDYASMPKNPTGFKITRYSIT 238
KNP G+++ Y
Sbjct: 200 TPSKEVDRFKNPLGYQVESYRAD 222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00200TYPE4SSCAGX320.004 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 31.7 bits (71), Expect = 0.004
Identities = 26/72 (36%), Positives = 37/72 (51%), Gaps = 8/72 (11%)

Query: 191 KEETKEEETITIGDNTNAMKIVKKDIQKGYRALKSSQ--RKWYCLGICSKKSKLSLMPEE 248
KE+ +EE+ I D A+ + Q + ALK + R + K+SK +MP E
Sbjct: 365 KEKIREEKQKIILDQAKAL-----ETQYVHNALKRNPVPRNYNYYQAPEKRSK-HIMPSE 418

Query: 249 IFNDKQFTYFKF 260
IF+D FTYF F
Sbjct: 419 IFDDGTFTYFGF 430


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00215NUCEPIMERASE881e-21 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 87.9 bits (218), Expect = 1e-21
Identities = 46/180 (25%), Positives = 72/180 (40%), Gaps = 19/180 (10%)

Query: 7 LITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGD 66
L+TG G G ++++ LL G++V G+ + + S E L F H D
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQP---GFQFHKID 60

Query: 67 MTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMRILGLEK 126
+ D + L A+ ++ + V+ S E P A+++ G L ILE R ++
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ- 119

Query: 127 KTRFYQASTSELYGEVLETPQNENTPF-------NPRSPYAVAKMYAFYITKNYREAYNL 179
AS+S +YG N PF +P S YA K + Y Y L
Sbjct: 120 --HLLYASSSSVYGL------NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00220NUCEPIMERASE504e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 50.2 bits (120), Expect = 4e-09
Identities = 52/346 (15%), Positives = 107/346 (30%), Gaps = 54/346 (15%)

Query: 5 ILITGAYGMVGKNTALYFKKNKPDV-----------ALLTPKKSELC-----------LL 42
L+TGA G +G + + + V L + EL L
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDLA 62

Query: 43 DKDNVQAYLKEYKPTGIIHCAGRVGGIVANMNDLSAYMVENLLMGLYLFSSALDLGVKKA 102
D++ + + R + ++ + AY NL L + ++
Sbjct: 63 DREGMTDLFASGHFERVFISPHR-LAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 103 INLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLV 162
+ +SS Y P D ++ + YA K + S G+ L
Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVSL----YAATKKANELMAHTYSHLYGLPATGLR 177

Query: 163 PCNLYGEFDKFEEKIAHMIPGLIARMHTAKLKNEKEFAMWGDGTARREYLNAKDLARFIS 222
+YG + + P + T + K ++ G +R++ D+A I
Sbjct: 178 FFTVYGPWGR---------PDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAII 228

Query: 223 LAYENIASIPS-----------------VMNVGSGVDYSIEEYYKMVAQVLDYKGAFVKD 265
+ I + V N+G+ + +Y + + L +
Sbjct: 229 RLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNML 288

Query: 266 LSKPVGMQQKLMDISK-QKALKWELEIPLEQGIKEAYEYYLKLLEV 310
+P + + D + + + E ++ G+K +Y +V
Sbjct: 289 PLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDFYKV 334


21HPSAT_00545HPSAT_00570N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_00545-1110.555870flagellin B
HPSAT_00550014-0.731353DNA topoisomerase I
HPSAT_00555011-0.649567hypothetical protein
HPSAT_00560011-0.633537hypothetical protein
HPSAT_005650100.548723hypothetical protein
HPSAT_005700121.912453phosphoenolpyruvate synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00545FLAGELLIN2851e-92 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 285 bits (731), Expect = 1e-92
Identities = 128/519 (24%), Positives = 219/519 (42%), Gaps = 18/519 (3%)

Query: 2 SFRINTNIAALTSHAVGVQNNRDLSSSLEKLSSGLRINKAADDSSGMAIADSLRSQSANL 61
+ INTN +L + ++ LSS++E+LSSGLRIN A DD++G AIA+ S L
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 GQAIRNANDAIGMVQTADKAMDEQIKILDTIKTKAVQAAQDGQTLESRRALQSDIQRLLE 121
QA RNAND I + QT + A++E L ++ +VQA + +++Q +IQ+ LE
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 ELDNIANTTSFNGQQMLSGSFSNKEFQIGAYSNTTVKASIGSTSSDKIGHVRMETSSFSG 181
E+D ++N T FNG ++LS + Q+GA T+ + +G +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDN-QMKIQVGANDGETITIDLQKIDVKSLGLDGFNVN---- 175

Query: 182 EGMLASAAAQNLTEVGLNFKQVNGVNDYKIETVRISTSAGTGIGALSEIINRFSNTLGVR 241
+ ++ +FK V G + Y + + +G + + V
Sbjct: 176 -----GPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVN 230

Query: 242 ASYNVMATG----GTPVQSGTVRDLTINGVEIGTVNDVHKNDADGRLINAINSVKDRTGV 297
A+ + T T V T E + K +G + V
Sbjct: 231 AANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFD-YKGVTFTIDT 289

Query: 298 EASLDIQGRINLHSIDGRAISVHATSASGQVFGGGNFAGISGTQHAVIGRLTLTRTDARD 357
+ D G+++ +I+G +++ + S D +
Sbjct: 290 KTGNDGNGKVST-TINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKT 348

Query: 358 IIVSGVNFSHVGFHSAQGVAEYTVNLRAVRGIFDANVASAAGANANGAQAETNSQGIGAG 417
S ++ +G ++ TVN + + AG + + +
Sbjct: 349 KNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINE 408

Query: 418 --VTSLKGAMIVMDMADSARTQLDKIRSDMGSVQMELVTTINNISVTQVNVKAAESQIRD 475
+ K + DSA +++D +RS +G++Q + I N+ T N+ +A S+I D
Sbjct: 409 DAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468

Query: 476 VDFAEESANFSKYNILAQSGSFAMAQANAVQQNVLRLLQ 514
D+A E +N SK IL Q+G+ +AQAN V QNVL LL+
Sbjct: 469 ADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLLR 507


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00560IGASERPTASE330.003 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.7 bits (74), Expect = 0.003
Identities = 31/228 (13%), Positives = 74/228 (32%), Gaps = 9/228 (3%)

Query: 98 QSKKEVAEAQKEAESARDRANKSGIELEQEKQKTSNIETSNQIKVEQEKQKTSNIETSNQ 157
Q+++ EA+ ++ + E ++ +T+ + + ++ ++EK K +T
Sbjct: 1064 QNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVE-KEEKAKVETEKTQEV 1122

Query: 158 IK-TEQEKQKTSNIETSN-QIKTEQERQKVEQEKQKTSKSGIELEQERQKTIKAQKDFIK 215
K T Q K ET Q + +E K+ S++ + E Q + + +
Sbjct: 1123 PKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTE-QPAKETSSNVEQ 1181

Query: 216 DLEQNCKENHGQFFIEKGGIKDSIAIEVEAECKTPKTTKTN-----QTPIQPKHLPNSKQ 270
+ ++ N G +E + ++ K ++ +
Sbjct: 1182 PVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSS 1241

Query: 271 PHSQRGSKAQELIAYLQKELESLPYSQKAIAKQVNFYRPSFIAYLELD 318
+ L + +A V I+ LE++
Sbjct: 1242 NDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQHISQLEMN 1289


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00565IGASERPTASE300.024 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 29.6 bits (66), Expect = 0.024
Identities = 29/172 (16%), Positives = 67/172 (38%), Gaps = 12/172 (6%)

Query: 92 ELEQEKQKTEQEKQKTEQEKQKTSNIETSNQIKVEQEKQKTNKSGIELEQERQKTIKKQK 151
E ++ ++EK K E EK + TS Q+ +QE+ +T + E +E T+
Sbjct: 1099 ETKETATVEKEEKAKVETEKTQEVPKVTS-QVSPKQEQSETVQPQAEPARENDPTV---- 1153

Query: 152 DFIKDLEQNCKENHGQFFIEKGGIKDSIAIEVEAECKTPKTTKTNQTPIQPKHLPNSEQP 211
++++ + + E+ + S +E T N P++ +
Sbjct: 1154 ----NIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTT-VNTGNSVVENPENTTPATTQ 1208

Query: 212 HSQRGSKAQELIAYLQKELESLPYSQKAIAKQVNFYRPSSIAYLELDPRDFN 263
+ + + ++ + S+P++ + N S++A +L + N
Sbjct: 1209 PTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSN--DRSTVALCDLTSTNTN 1258


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_00570PHPHTRNFRASE2947e-92 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 294 bits (753), Expect = 7e-92
Identities = 105/441 (23%), Positives = 185/441 (41%), Gaps = 67/441 (15%)

Query: 388 DLEHMNSFKEGEILVTDN-TDPDWEPCMKK-ASAVITNRGGRTCHAAIVAREIGVPAIVG 445
+ + + E +++ ++ T D K+ T+ GGRT H+AI++R + +PA+VG
Sbjct: 146 ETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMSRSLEIPAVVG 205

Query: 446 VSGATDSLYTGMEITVSCAEGE---------EGYVYAGIYEHEIERVELSNMQETQT--- 493
T+ + G + V EG E ++ E + + +
Sbjct: 206 TKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYEEKRAAFEKQKQEWAKLVGEPSTTK 265

Query: 494 -----KIYINIGNPEKAFGFSQLPNHGVGLARMEMIILNQIKAHPLALVDLHHKKSVKEK 548
++ NIG P+ G G+GL R E + +++ + P
Sbjct: 266 DGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFLYMDRDQ-LPTE------------- 311

Query: 549 NEIENLMAGYANPKDFFVKKIAEGIGMISAAFYPKPVIVRTSDFKSNEYMRMLGGSSYEP 608
E Y K++ + KPV++RT D ++ + L P
Sbjct: 312 ---EEQFEAY--------KEVVQ-------RMDGKPVVIRTLDIGGDKELSYL----QLP 349

Query: 609 NEENPMLGYRGASRYYSESYNEAFSWECEALALVREEMGLTNMKVMIPFLRTIEEGKKVL 668
E NP LG+R + F + AL N+KVM P + T+EE ++
Sbjct: 350 KELNPFLGFRAIRLCLE--KQDIFRTQLRALL---RASTYGNLKVMFPMIATLEELRQAK 404

Query: 669 EILRKNNLESGKNG------LEIYIMCELPVNVILADDFLSLFDGFSIGSNDLTQLTLGV 722
I+++ + G +E+ IM E+P + A+ F D FSIG+NDL Q T+
Sbjct: 405 AIMQEEKDKLLSEGVDVSDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAA 464

Query: 723 DRDSELVSHVFDERNGAMLKMFKKAIEACKRHNKYCGICGQAPSDYPEVTEFLVKEGITS 782
DR +E VS+++ + A+L++ I+A K+ G+CG+ D L+ G+
Sbjct: 465 DRMNERVSYLYQPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGD-EVAIPLLLGLGLDE 523

Query: 783 ISLNPDSVIPTWNAVAKLEKE 803
S++ S++P + + KL KE
Sbjct: 524 FSMSATSILPARSQLLKLSKE 544


22HPSAT_01215HPSAT_01250N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_01215-2130.884344neutrophil activating protein (napA)
HPSAT_01220-2120.658294histidine kinase sensor protein
HPSAT_01225-2111.430286hypothetical protein
HPSAT_01230-2111.999761flagellar basal body P-ring protein
HPSAT_01235-1121.778605ATP-dependent RNA helicase
HPSAT_01240-2101.429216hypothetical protein
HPSAT_01245-2110.986382hypothetical protein
HPSAT_01250-2111.954721oligopeptide permease ATPase protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01215HELNAPAPROT1485e-49 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 148 bits (376), Expect = 5e-49
Identities = 39/140 (27%), Positives = 75/140 (53%), Gaps = 1/140 (0%)

Query: 5 EILKHLQADAIVLFMKVHNFHWNVKGTDFFNVHKATEEIYEEFADMFDDLAERIVQLGHH 64
L ++ +L+ K+H FHW VKG FF +H+ EE+Y+ A+ D +AER++ +G
Sbjct: 15 NSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIGGQ 74

Query: 65 PLVTLSEAIKLTRVKEETKTSFHSKDIFKEILEDYKHLEKEFKTLSNTAEKEGDKVTVTY 124
P+ T+ E + + + + + ++ + ++ DYK + E K + AE+ D T
Sbjct: 75 PVATVKEYTEHASITDGGNET-SASEMVQALVNDYKQISSESKFVIGLAEENQDNATADL 133

Query: 125 ADDQLAKLQKSIWMLQAHLA 144
+ +++K +WML ++L
Sbjct: 134 FVGLIEEVEKQVWMLSSYLG 153


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01220PF06580300.015 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.8 bits (67), Expect = 0.015
Identities = 10/71 (14%), Positives = 25/71 (35%), Gaps = 13/71 (18%)

Query: 281 IVLQNFLYNAIDAIEALEESEQ-GQVKIEAFIQNEFIVFTIIDNGKEVENKSALFEPFET 339
+++Q + N I + + Q G++ ++ N + + + G +
Sbjct: 258 MLVQTLVENGI--KHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK------- 308

Query: 340 TKLKGNGLGLA 350
+ G GL
Sbjct: 309 ---ESTGTGLQ 316


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01230FLGPRINGFLGI358e-125 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 358 bits (920), Expect = e-125
Identities = 118/345 (34%), Positives = 191/345 (55%), Gaps = 26/345 (7%)

Query: 19 AEKIGDIASVVGVRDNQLIGYGLVIGLNGTGDK-SGSKFTMQSISNMLESVNVKISADDI 77
+I DIAS+ RDNQLIGYGLV+GL GTGD S FT QS+ ML+++ +
Sbjct: 28 TSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQS 87

Query: 78 KSKNVAAVMITASLPPFARQGDKIDVQISSIGDAKSIQGGTLVMTPLNAVDGNIYALAQG 137
+KN+AAVM+TA+LPPFA G ++DV +SS+GDA S++GG L+MT L+ DG IYA+AQG
Sbjct: 88 NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQG 147

Query: 138 AITSGN-----------SNNLLSANIINGATIEREVSYDLFHKNAMVLSLKSPNFKNAIQ 186
A+ SA + NGA IERE+ +VL L++P+F A++
Sbjct: 148 ALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPDFSTAVR 207

Query: 187 VQNTLNKV----FGNKVAIALDPKTIQITRPERFSMVEFLALVQEIPINYSAKNKIIVDE 242
V + +N +G+ +A D + I + +P + +A ++ + + K++++E
Sbjct: 208 VADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPAKVVINE 267

Query: 243 KSGTIVSGVDIMVHPIVVTSQDITLKITKEPLDN--------SKNAQDLDNNMSLDTAHN 294
++GTIV G D+ + + V+ +T+++T+ P Q + M++
Sbjct: 268 RTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGSK 327

Query: 295 TLSSNGKNITIAGVVKALQKIGVSAKGMVSILQALKKSGAISAEM 339
G ++ +V L IG+ A G+++ILQ +K +GA+ AE+
Sbjct: 328 VAIVEGPDLR--TLVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01235SECA300.027 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.027
Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 2/63 (3%)

Query: 261 IVFTRTKKEADELHQFLASKNYKSTALHGDMDQRDRRASIMAFKKNDADVLVATDVASRG 320
+V T + ++++ + L K L+ + A+I+A A V +AT++A RG
Sbjct: 453 LVGTISIEKSELVSNELTKAGIKHNVLNAKFHANE--AAIVAQAGYPAAVTIATNMAGRG 510

Query: 321 LDI 323
DI
Sbjct: 511 TDI 513


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01250HTHFIS320.006 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 32.1 bits (73), Expect = 0.006
Identities = 16/50 (32%), Positives = 21/50 (42%), Gaps = 7/50 (14%)

Query: 30 VAIVGESGSGKSSIANIIMRLNPR----FKPHNGEVLFETTNLLKESEAF 75
+ I GESG+GK +A + R F N + L ESE F
Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRD---LIESELF 209


23HPSAT_01750HPSAT_01790N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_01750-390.726823flagellar motor switch protein G
HPSAT_01755-2100.829923flagellar assembly protein H
HPSAT_01760-191.1321351-deoxy-D-xylulose-5-phosphate synthase
HPSAT_017650110.523592GTP-binding protein LepA
HPSAT_01770013-1.325716DNA-cytosine methyltransferase
HPSAT_01775-1110.303674hypothetical protein
HPSAT_01780012-0.033460flagellar basal-body rod protein
HPSAT_01785111-0.420755alpha-ketoglutarate permease
HPSAT_01790012-0.553286DNA translocase FtsK; putative membrane protein;
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01750FLGMOTORFLIG350e-122 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 350 bits (900), Expect = e-122
Identities = 121/338 (35%), Positives = 208/338 (61%), Gaps = 4/338 (1%)

Query: 8 KQKAQLDELSMSEKIAILLIQVGEDTTGEILRHLDIDSITEISKQIVQLNGTDKQIGAAV 67
K+ + L+ +K AILL+ +G + + ++ ++L + I ++ +I +L ++ V
Sbjct: 7 KEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNV 66

Query: 68 LEEFFAIFQSNQYINTGGLEYARELLTRTLGSEEAKKVMDKLTKSLQTQKNFAYLGKIKP 127
L EF + + ++I GG++YARELL ++LG+++A +++ L +LQ+ + F ++ + P
Sbjct: 67 LLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQS-RPFEFVRRADP 125

Query: 128 QQLADFIINEHPQTIALILAHMEAPNAAETLSYFPDEMKAEISIRMANLGEISPQVVKRV 187
+ +FI EHPQTIALIL++++ A+ LS P E++ ++ R+A + SP+VV+ V
Sbjct: 126 ANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREV 185

Query: 188 STVLENKLESLTSYK-IEVGGLRAVAEIFNRLGQKSAKTTLARIESVDNKLAGAIKEMMF 246
VLE KL SL+S GG+ V EI N +K+ K + +E D +LA IK+ MF
Sbjct: 186 ERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMF 245

Query: 247 TFEDISKLDNFAIREILKVADKKDLSLALKTSTQDLTDKFLNNMSSRAAEQFVEEMQYLG 306
FEDI LD+ +I+ +L+ D ++L+ ALK+ + +K NMS RAA E+M++LG
Sbjct: 246 VFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLG 305

Query: 307 AVKIKDVDVAQRKIIEIVQSLQEKG--VIQTGEEEDVI 342
+ KDV+ +Q+KI+ +++ L+E+G VI G EEDV+
Sbjct: 306 PTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVL 343



Score = 30.2 bits (68), Expect = 0.010
Identities = 20/102 (19%), Positives = 41/102 (40%), Gaps = 3/102 (2%)

Query: 4 KLTPKQKAQLDELSMSEKIAILLIQVGEDTTGEILRHLDIDSITEISKQIVQLNGTDKQI 63
+ P + + IA++L + IL L + T ++++I ++ T ++
Sbjct: 122 RADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEV 181

Query: 64 GAA---VLEEFFAIFQSNQYINTGGLEYARELLTRTLGSEEA 102
VLE+ A S Y + GG++ E++ E
Sbjct: 182 VREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEK 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01755FLGFLIH374e-05 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 37.1 bits (85), Expect = 4e-05
Identities = 46/212 (21%), Positives = 95/212 (44%), Gaps = 17/212 (8%)

Query: 43 PNPEEPLEKKAIENDLIDCLLKKTDELSSHLVKLQMQFEKAQEES-KALIENAKNDGYKI 101
P E + E +I+ + L L +LQMQ A E+ +A I + G+K
Sbjct: 17 PPQAEFVPIVEPEETIIE---EAEPSLEQQLAQLQMQ---AHEQGYQAGIAEGRQQGHKQ 70

Query: 102 GFKEGEEKMRNELTHSVNEEKNQLLHAITALDEKMKKSEDHLMALE----KELSAIAIDI 157
G++EG + L + E K+Q + + + + + L AL+ L +A++
Sbjct: 71 GYQEG---LAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEA 127

Query: 158 AKEVILKEVEDNSQKVALALAEELLKNVLDATDIHLKVNPLDYPYLNERLQNASKI---K 214
A++VI + ++ + + + L + L + L+V+P D +++ L + +
Sbjct: 128 ARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGATLSLHGWR 187

Query: 215 LESNEAISKGGVMITSSNGSLDGNLMERFKTL 246
L + + GG +++ G LD ++ R++ L
Sbjct: 188 LRGDPTLHPGGCKVSADEGDLDASVATRWQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01765TCRTETOQM1425e-38 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 142 bits (359), Expect = 5e-38
Identities = 99/437 (22%), Positives = 174/437 (39%), Gaps = 85/437 (19%)

Query: 9 NIRNFSIIAHIDHGKSTLADCLIAECNAIS---NREMTSQVMDTMDIEKERGITIKAQSV 65
I N ++AH+D GK+TL + L+ AI+ + + + D +E++RGITI+
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 66 RLNYTLKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANVYIAL 125
+ E+ +N+IDTPGH+DF EV RSL +GA+L++ A GV+AQT +
Sbjct: 62 SFQW----ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 126 DNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDCSNANEVSAKARLGIKD--------- 176
+ + INKID ++ V QDI++ + + +V + + +
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDT 177

Query: 177 -------LLEKIITTIPAPSGDFNAPLKALIYD-------------------------SW 204
LLEK ++ + + ++ +
Sbjct: 178 VIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNK 237

Query: 205 F--------------------DNYLGALALVRIMDGSINTEQEILVMGTGKKHGVLGLYY 244
F LA +R+ G ++ + + K + +Y
Sbjct: 238 FYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYT 296

Query: 245 PNPLKKIPTKNLECGEIGIV---SLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPFV 301
+ GEI I+ L L SV +GDT P + IE P +
Sbjct: 297 SINGELCKIDKAYSGEIVILQNEFLKLNSV----LGDTKLL---PQRERIEN---PLPLL 346

Query: 302 FAGLYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERL 361
+ P + + E L +ALL++ +D L + +S+ + FLG + MEV L
Sbjct: 347 QTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATH---EIILSFLGKVQMEVTCALL 403

Query: 362 EREFGLNLIATAPTVVY 378
+ ++ + + PTV+Y
Sbjct: 404 QEKYHVEIEIKEPTVIY 420



Score = 31.0 bits (70), Expect = 0.015
Identities = 15/75 (20%), Positives = 28/75 (37%), Gaps = 2/75 (2%)

Query: 405 IKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDK 464
+ EP++ I P E+L + L + V+L+ +P+ I ++
Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCI-QEYRSD 592

Query: 465 LKSCTKGYASFDYEP 479
L T G + E
Sbjct: 593 LTFFTNGRSVCLTEL 607


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01780FLGHOOKAP1300.009 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 30.3 bits (68), Expect = 0.009
Identities = 9/40 (22%), Positives = 17/40 (42%)

Query: 3 NGYYAATGAMVAQFNRLDLTSNNLANLNTNGFKRDDAVTG 42
+ A + A L+ SNN+++ N G+ R +
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMA 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01785TCRTETB418e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 41.0 bits (96), Expect = 8e-06
Identities = 59/315 (18%), Positives = 104/315 (33%), Gaps = 67/315 (21%)

Query: 37 APYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKKGRKTSMVYSIILMA 96
P A +F T + +AF++ G+ +GKL D+ G K +++ II+
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSI------GTAVYGKLSDQLGIKRLLLFGIIINC 90

Query: 97 LGSFMLALLPTKEIVGEWAFLFLLLARLLQGFSVGGE------YGVVATYLSELGKNGKK 150
GS + VG F L++AR +QG G VVA Y+ + +
Sbjct: 91 FGSVIGF-------VGHSFFSLLIMARFIQG--AGAAAFPALVMVVVARYIPKENRGKAF 141

Query: 151 GFYGSFQYITLVGGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIM 210
G GS I +G + I I+ W YL + I + FL ++
Sbjct: 142 GLIGS---IVAMGEGVGPAIGGMIAHYIH---------WSYLLLIPMITIITVPFLMKLL 189

Query: 211 EETMDSQTTSKTTIKEETQRGSLKELLNHKKALM-------IVFGLTMGGSLCFYTFTVY 263
+ + +K + K ++ + T +
Sbjct: 190 K-----------------KEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLS 232

Query: 264 LKIFLTNSSSFSPK-------ESSFIMLLALSYFIFLQPLCG---MLADKIKRTQMLMVF 313
IF+ + + ++ M+ L I + G M+ +K L
Sbjct: 233 FLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA 292

Query: 314 AIAGLIVTPVVFYGI 328
I +I+ P I
Sbjct: 293 EIGSVIIFPGTMSVI 307


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01790IGASERPTASE330.005 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 33.5 bits (76), Expect = 0.005
Identities = 48/234 (20%), Positives = 78/234 (33%), Gaps = 44/234 (18%)

Query: 179 APSDIQKKETK---NDKEKENLKENPI-DENHKTPNEESLLAIPTPYNTTLNALEPQEGL 234
P++IQ N++E + E P+ TP+E + + +E E
Sbjct: 999 TPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQES-KTVEKNE-- 1055

Query: 235 VQISSNPPTHYTIYPKKNRFDDLSNPTNPTLKEVKQETKEREPTLKKET----------- 283
Q ++ K+ + + +N + + ETKE + T KET
Sbjct: 1056 -QDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKV 1114

Query: 284 --------------LTPATLKP--IMPASAPNTENDNKTENHKAPNHPTKEENVQENAQE 327
++P + + P + P END T N K P T E +
Sbjct: 1115 ETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREND-PTVNIKEPQSQTNTTADTEQPAK 1173

Query: 328 EMIKENIKEMIEERIKENIKEEEKEAQNAPSFNPITPTSAKKPVMVKELSENKE 381
E N+++ + E N N+ NP T A V S NK
Sbjct: 1174 E-TSSNVEQPVTESTTVN-------TGNSVVENPENTTPATTQPTVNSESSNKP 1219


24HPSAT_01875HPSAT_01910N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_01875-1161.029405hypothetical protein
HPSAT_01880-1150.903650copper ion binding protein
HPSAT_01885-1140.942208copper-transporting ATPase
HPSAT_01890-2130.536019phosphatidylserine synthase
HPSAT_01895-213-0.142556hypothetical protein
HPSAT_01900-2120.161834cell division protein (ftsH)
HPSAT_01905-114-2.451162ribosomal protein L11 methyltransferase
HPSAT_01910-112-2.292021response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01875STREPTOPAIN280.004 Streptopain (C10) cysteine protease family signature.
		>STREPTOPAIN#Streptopain (C10) cysteine protease family signature.

Length = 398

Score = 28.1 bits (62), Expect = 0.004
Identities = 18/61 (29%), Positives = 27/61 (44%)

Query: 2 KIKAIMLGLVMSGGLLLANGEQDAYNFKAMEKEIKEITKMIITTGEAVKMNEKQFDQLNA 61
K+ +L L+ GG +LAN NF EKE K+ I A+K + + +
Sbjct: 5 KLGVRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAITFIQKSAAIKAGARSAEDIKL 64

Query: 62 D 62
D
Sbjct: 65 D 65


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01885SECYTRNLCASE320.008 Preprotein translocase SecY subunit signature.
		>SECYTRNLCASE#Preprotein translocase SecY subunit signature.

Length = 437

Score = 32.0 bits (73), Expect = 0.008
Identities = 20/124 (16%), Positives = 41/124 (33%), Gaps = 14/124 (11%)

Query: 86 LAVVFTLF-----VVYLSMGAMLSPSLLPESLLTIDNHSNFLNACLQL-IGALIVMHLGR 139
L V + V + + ++ + + + + G +VM LG
Sbjct: 120 LTVALAILQGTGLVATARSAPLFGRCSVGGQIVPDQSIFTTITMVICMTAGTCVVMWLGE 179

Query: 140 DFYIQGFKALWHRQPNMSSLIAIGTSAALISSLWPLYLVYTNQWSYGHYYFESVCVILMF 199
+G MS L+ I +A S+LW + + G F +V + +
Sbjct: 180 LITDRGIGN------GMSILMFISIAATFPSALWAIK--KQGTLAGGWIEFGTVIAVGLI 231

Query: 200 VMVG 203
++
Sbjct: 232 MVAL 235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01900HTHFIS340.001 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.4 bits (79), Expect = 0.001
Identities = 26/131 (19%), Positives = 49/131 (37%), Gaps = 28/131 (21%)

Query: 157 KKLINAEKPNVRFNDMAGNEEAKEEVVEIVDFLKYPERYANLGAKIPKGVLLVGPPGTGK 216
++ E + + G A +E+ ++ R +++ G GTGK
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLA------RLMQTDLT----LMITGESGTGK 173

Query: 217 TLLAKAV---AGEAHVPFFSMGGSSF------IEMF-----VGLGASRVRD-LFETAKKQ 261
L+A+A+ + PF ++ ++ E+F GA FE A+
Sbjct: 174 ELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGG 233

Query: 262 APSIIFIDEID 272
+F+DEI
Sbjct: 234 T---LFLDEIG 241


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_01910HTHFIS865e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.0 bits (213), Expect = 5e-23
Identities = 30/109 (27%), Positives = 57/109 (52%), Gaps = 4/109 (3%)

Query: 2 KLLVVDDSSTMRRIIKNTLSRLGYEDVLEAEHGVEAWEKLNANADTKVLITDWNMPEMNG 61
+LV DD + +R ++ LSR GY DV + W + A D +++TD MP+ N
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGY-DVRITSNAATLWRWI-AAGDGDLVVTDVVMPDENA 62

Query: 62 LDLVIKVRADERFKEIPIIMITTEGGKAEVITALKAGVNNYIVKPFTPQ 110
DL+ +++ + ++P+++++ + I A + G +Y+ KPF
Sbjct: 63 FDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


25HPSAT_03695HPSAT_03785N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_03695-112-1.426117putative lipopolysaccharide biosynthesis
HPSAT_03700-112-0.892914putative lipopolysaccharide biosynthesis
HPSAT_03705-111-0.813092putative lipopolysaccharide biosynthesis
HPSAT_03710-112-0.433333adenylate kinase
HPSAT_03715-212-1.697231aspartyl-tRNA synthetase
HPSAT_03720110-1.197679chemotaxis protein
HPSAT_03725210-1.179917NAD-dependent DNA ligase LigA
HPSAT_0373028-0.374911hypothetical protein
HPSAT_0373528-0.257820putative abc transporter, ATP-binding protein
HPSAT_0374018-0.132759ABC-type multidrug transport system, permease
HPSAT_03745180.350085putative vacuolating cytotoxin (VacA)-like
HPSAT_03750-2111.215247hypothetical protein
HPSAT_03755-3100.781321cytoplasmic pump protein of the hefABC efflux
HPSAT_03760-3110.509529membrane fusion protein of the hefABC efflux
HPSAT_03765-210-0.431579outer-membrane protein of the hefABC efflux
HPSAT_03770-211-0.433422uroporphyrinogen decarboxylase
HPSAT_03775-211-0.879085hypothetical protein
HPSAT_03780010-0.3714173-methyladenine DNA glycosylase
HPSAT_037851100.086728flagellin A
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03695BACINVASINB300.008 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 30.1 bits (67), Expect = 0.008
Identities = 21/111 (18%), Positives = 38/111 (34%), Gaps = 7/111 (6%)

Query: 32 KIKRKLNHFIGNILARTEVYKKLVAKYDELTGKYDDLTGKYDDLTGKYDDL---TGKYDD 88
++ ++ +G T++Y+ + K D YD T K K L Y
Sbjct: 124 QVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAATKKLTQAQNKLQSLDPADPGYAQ 183

Query: 89 ----LTGKYDDLTGKYESLLAKEANIKETFWERRADSEKEALFLEHFYLTS 135
+ + T E+L + + +A +EK L F T+
Sbjct: 184 AEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTA 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03710MALTOSEBP290.017 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 28.5 bits (63), Expect = 0.017
Identities = 20/52 (38%), Positives = 30/52 (57%), Gaps = 5/52 (9%)

Query: 60 GELVPLEIVVETILSAIKSSDKGIILIDGYPRSVEQMQALDKELSAQNEVVL 111
G+L+ I VE LS I + D L+ P++ E++ ALDKEL A+ + L
Sbjct: 127 GKLIAYPIAVEA-LSLIYNKD----LLPNPPKTWEEIPALDKELKAKGKSAL 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03720HTHFIS552e-10 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 54.8 bits (132), Expect = 2e-10
Identities = 23/110 (20%), Positives = 44/110 (40%), Gaps = 6/110 (5%)

Query: 194 ILIAEDSLSALKTLEKIVQTLELRYLAFPNGRELLDYLYEKEHYQQVGVVITDLEMPVIS 253
IL+A+D + L + + N L ++ +V+TD+ MP +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAA----GDGDLVVTDVVMPDEN 61

Query: 254 GFEVLKTIKADSRTEHIPVIINSSMSSDSNRQLAQSLEADGFVVKSNILE 303
F++L IK +PV++ S+ ++ A A ++ K L
Sbjct: 62 AFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03730LCRVANTIGEN290.003 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 28.9 bits (64), Expect = 0.003
Identities = 15/33 (45%), Positives = 20/33 (60%)

Query: 16 KRKKLLTELAELEAEIKVSSERKSSFSISLSPS 48
R KL ELAEL AE+K+ S ++ + LS S
Sbjct: 149 ARSKLREELAELTAELKIYSVIQAEINKHLSSS 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03745VACCYTOTOXIN2787e-78 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 278 bits (713), Expect = 7e-78
Identities = 106/394 (26%), Positives = 181/394 (45%), Gaps = 14/394 (3%)

Query: 2796 NAVNWLNALFVAKGGNPLFAPYYLQDTPTEHIVTLMKDITGALGMLAKPNLKNNSTDVLQ 2855
+ L L + + +A + T I + T L +A K + L
Sbjct: 907 QGRDLLQTLLI-DSHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLS 965

Query: 2856 LNTYTQQMGRLAKLSNFASFDSTDFSERLSSLKNQRFADAIPNAMDVILKYSQRDKLKNN 2915
L+ RL LS + F++RL +LK+QRFA + +A +V+ +++ + + N
Sbjct: 966 LSNAMILNSRLVNLSRRHTNHIDSFAKRLQALKDQRFAS-LESAAEVLYQFAPKYEKPTN 1024

Query: 2916 LWATGVGGVSFVENGTGTLYGVNVGYDRFVKG---VIVGGYAAYGYSGFYER--ITSSKS 2970
+WA +GG S G +LYG + G D ++ G IVGG+ +YGYS F + +S +
Sbjct: 1025 VWANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGA 1084

Query: 2971 DNVDVGLYARAFIKKSELTFSVNETWGANKTQISSNDALLSMINQSYQYNTWTTNARVNY 3030
+N + G+Y+R F + E F G++++ ++ ALL +NQSY Y ++ R +Y
Sbjct: 1085 NNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASY 1144

Query: 3031 GYDFMFKNKSIILKPQIGLRYYYIGMTGLEGVMNNALYNQFKANADPSKKSVLTIDFAFE 3090
GYDF F +++LKP +G+ Y ++G T + + S + + E
Sbjct: 1145 GYDFAFFRNALVLKPSVGVSYNHLGSTNFKS----NSNQKVALKNGASSQHLFNASANVE 1200

Query: 3091 NRHYFNKNSYFYAIGGIGRDLLVRSMGDKLVRFIGDNTLSYRKGELYNTFASITTGGEVR 3150
R+Y+ SYFY G+ ++ + V + R NT A + GGE++
Sbjct: 1201 ARYYYGDTSYFYMNAGVLQEFANFGSSNA-VSLNTFKVNATRNP--LNTHARVMMGGELK 1257

Query: 3151 LFKSFYANAGVGARFGLDYKMINIIGNIGMRLAF 3184
L K + N G L + + N+GMR +F
Sbjct: 1258 LAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF 1291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03755ACRIFLAVINRP8960.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 896 bits (2316), Expect = 0.0
Identities = 286/1040 (27%), Positives = 518/1040 (49%), Gaps = 42/1040 (4%)

Query: 1 MYKTAINRPITTLMFALAIVFFGTMGFKKLSVALFPKIDMPTVVVTTTYPGASAEIIESK 60
M I RPI + A+ ++ G + +L VA +P I P V V+ YPGA A+ ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTDKIEEAVMGIDGIKKVTSTSSKNVSIVV-IEFELEKPNEEALNDVVNKISSVR-FDDS 118
VT IE+ + GID + ++STS S+ + + F+ + A V NK+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 119 NIKKPSINKFDTDSQAIISLFVSSSSVPAT--TLNDYAKNTIKPMLQKINGVGGVQLNGF 176
+++ I+ + S ++ S + T ++DY + +K L ++NGVG VQL G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 177 RERQIRIYADPTLMNKYNLTYADLFSTLKAENVEIDGGRIVNS------QRELSILINAN 230
+ +RI+ D L+NKY LT D+ + LK +N +I G++ + Q SI+
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 231 SYSVADVEKIQV-----GNHVRLGDIAKIEIGLEEDNTFASFKDKPGVILEIQKIAGANE 285
+ + K+ + G+ VRL D+A++E+G E N A KP L I+ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 286 IEIVDRVYEALKHIQAISP-SYEIRPFLDTTGYIRTSIEDVKFDLVLGAILAVLVVFAFL 344
++ + L +Q P ++ DTT +++ SI +V L +L LV++ FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 345 RNGTITLVSAISIPISIMGTFALIQWMGFSLNMLTMVALTLAIGIIIDDAIVVIENIHK- 403
+N TL+ I++P+ ++GTFA++ G+S+N LTM + LAIG+++DDAIVV+EN+ +
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 404 KLEMGMNKRKASYEGVREIGFALVAISAMLLSVFVPIGNMKGIIGRFFQSFGITVALAIA 463
+E + ++A+ + + +I ALV I+ +L +VF+P+ G G ++ F IT+ A+A
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 464 LSYVVVVTIIPMVSSVVVNPRHS-------RFYVWSEPFFKALESYYTRLLQWVLNHKLI 516
LS +V + + P + + ++ P + F+ W F ++YT + +L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 517 IFIAVVLVFVGSLFVASKIGMEFMLKEDRGRFLVWLKAKPGVSIDY----MTQKSKIFQK 572
+ L+ G + + ++ F+ +ED+G FL ++ G + + + Q + + K
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 573 AIEKHAEVEFTTLQVGY-GTAQNPFKAKIFVQLKPLKERKKERKLGQFELMSALRKELKS 631
+ + E FT + G AQN FV LKP +ER + ++ + EL
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNA--GMAFVSLKPWEERNGDENS-AEAVIHRAKMELGK 656

Query: 632 MPEAKGLESINLSEVSLIGGGGDSSPFQTFVFSHSQEAVDKSVANLKKFLLESPELKGKV 691
+ + + N+ + G ++ F + + D + L + + +
Sbjct: 657 IRDGF-VIPFNMPAIV---ELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASL 712

Query: 692 ESYHTSTSESQPQLQLKILRQNANKYGVSAQTIGSVVSSAFSGTSQASVFKQDGKEYDMI 751
S + E Q +L++ ++ A GVS I +S+A G + + F G+ +
Sbjct: 713 VSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGG-TYVNDFIDRGRVKKLY 771

Query: 752 IRVPDNKRVSVEDIKRLQVRNKYDKLMFLDALVEITETKSPSSISRYNRQRSVTVLAEPN 811
++ R+ ED+ +L VR+ +++ A + RYN S+ + E
Sbjct: 772 VQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA- 830

Query: 812 RNAGVSLGEILTQVSKNTKEWLVEGANYRFTGEADNAKETNGEFLIALATAFVLIYMILA 871
G S G+ + + +N L G Y +TG + + + + +A +FV++++ LA
Sbjct: 831 -APGTSSGDAMALM-ENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLA 888

Query: 872 ALYESILEPFIIMVTMPLSFSGAFFALGLVHQPLSMFSMIGLILLIGMVGKNATLLIDVA 931
ALYES P +M+ +PL G A L +Q ++ M+GL+ IG+ KNA L+++ A
Sbjct: 889 ALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFA 948

Query: 932 NE-ERKKGLNIQEAILFAGKTRLRPILMTTIAMVCGMLPLALASGDGAAMKSPIGIAMSG 990
+ K+G + EA L A + RLRPILMT++A + G+LPLA+++G G+ ++ +GI + G
Sbjct: 949 KDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008

Query: 991 GLMISMVLSLLIVPVFYRLL 1010
G++ + +L++ VPVF+ ++
Sbjct: 1009 GMVSATLLAIFFVPVFFVVI 1028


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03760RTXTOXIND549e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 54.1 bits (130), Expect = 9e-11
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 5/82 (6%)

Query: 27 NVKAVQDSKLTLDSTGIVDSIKVTEGSVVKKGDVLLLLYNQDKQAQSDSTEQQLIFAKKQ 86
K ++ IV I V EG V+KGDVLL L +A + T+ L+ A+ +
Sbjct: 95 RSKEIKPI-----ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLE 149

Query: 87 YQRYSKIGGAVDRNTLESYEFN 108
RY + +++ N L +
Sbjct: 150 QTRYQILSRSIELNKLPELKLP 171



Score = 32.1 bits (73), Expect = 0.002
Identities = 21/152 (13%), Positives = 49/152 (32%), Gaps = 25/152 (16%)

Query: 70 QAQSDSTEQQLIFAKKQYQR--YSKIGGAVDRNTLESYEFNYRRLESDYAYSIAVLNKTI 127
+++ S +++ + ++ K+ D + + E ++
Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTTDN--IGLLTLELAKNEER-------QQASV 329

Query: 128 LRAPFDGVIASKNIQVGEGVSANSTVLLRLVSHARKLVIE--FDSKYINAVKVG------ 179
+RAP + + GV + L+ +V L + +K I + VG
Sbjct: 330 IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIK 389

Query: 180 -DTYTYSIDGDSNQHEAKITKIYP--TVDENT 208
+ + Y+ G K+ I D+
Sbjct: 390 VEAFPYTRYGYL---VGKVKNINLDAIEDQRL 418


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03765RTXTOXIND310.016 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.016
Identities = 17/113 (15%), Positives = 41/113 (36%), Gaps = 16/113 (14%)

Query: 203 LARMIALQKKLEQIKTDIKRVTKLYDKGLTTIDDL-----QSLKAQGNLSEY--DILDIQ 255
LAR+ + K+ + + L K + + ++A L Y + I+
Sbjct: 220 LARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIE 279

Query: 256 FALEQNRLTLEYLTNLSVKNLKKTTIDAPNLQLRERQD-LVSLREQISALKYQ 307
+ + + +T K +D +LR+ D + L +++ + +
Sbjct: 280 SEILSAKEEYQLVTQ----LFKNEILD----KLRQTTDNIGLLTLELAKNEER 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03780PF05272300.011 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.011
Identities = 13/95 (13%), Positives = 26/95 (27%), Gaps = 20/95 (21%)

Query: 60 ILENDDEINLKKIAYIEFSKLAECVRPSGFYNQKAKRLIDLSGNILKDFQSFENFKQEVT 119
L + + +A+ E + VR + +KA E+
Sbjct: 458 ALRSAPALA-GCVAFDELREQPVAVRAFPW--RKAPGP-------------LEDADVLRL 501

Query: 120 REWLLDQKGIGKESADAILCYVCAKEVMVVDKYSY 154
+++ G G+ SA + D
Sbjct: 502 ADYVETTYGTGEASAQTTEQAINV----AADMNRV 532


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03785FLAGELLIN2446e-77 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 244 bits (624), Expect = 6e-77
Identities = 127/518 (24%), Positives = 210/518 (40%), Gaps = 22/518 (4%)

Query: 2 AFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQANSL 61
A +NTN ++ +Q++L +++ERLSSGLRIN A DDA+G +A+ S L
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 GQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLIQ 121
QA N NDG+ I Q + A++E L V+ + QA + K+IQ +I + ++
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 GLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRI-ATGALI 180
+D + N T +NG +LS + QVGA ++I + +G G
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDN-QMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 181 TASGDISLTFKQVDGVNDVTLESVKISSSAGTGIGVLAEVINKNSNQTGVRAYANVITTS 240
GD+ +FK V G + + + K +G V ++ V A +TT
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 241 DVAVQSGSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQNGRLNLR 300
D N + K A A+ + + + +
Sbjct: 240 DAE-----------NNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTID 288

Query: 301 SLDGRGIEIKTDSTSNGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINV------VS 354
+ G K +T NG V S + +N +
Sbjct: 289 TKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKT 348

Query: 355 ASDSQHLGFSAIGFGESQVAETTVNLRDVTGNFNANVKSASGANYNAVLASGNQSL---G 411
++S L ++ TVN + T N + + +G + S
Sbjct: 349 KNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINE 408

Query: 412 AGVTTLRGAMVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRD 471
+ + +SA+ +D VRS LG++QN+ S + N+ T N+ +A S+I D
Sbjct: 409 DAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468

Query: 472 VDFAEESANFNKNNILAQSGSYAMSQANTVQQNILRLL 509
D+A E +N +K IL Q+G+ ++QAN V QN+L LL
Sbjct: 469 ADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


26HPSAT_03880HPSAT_03910N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_03880215-0.760766endonuclease III
HPSAT_03885214-1.476698flagellar motor switch protein
HPSAT_03890113-1.845236hypothetical protein
HPSAT_03895112-0.918389hypothetical protein
HPSAT_03900-113-0.817026dihydroorotase
HPSAT_03905012-0.751494hypothetical protein
HPSAT_03910-111-0.558480hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03880OMS28PORIN290.014 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 29.0 bits (64), Expect = 0.014
Identities = 29/112 (25%), Positives = 54/112 (48%), Gaps = 11/112 (9%)

Query: 27 NQTTELHHKNPYELLVATILSAQCTDARVNKITPKLFEKYPSVKDLAL-----ASLEGVK 81
N+ E+ K E A ++ + T +I + K P+ K+L L A +E VK
Sbjct: 132 NKVVEMSKKAVQETQKAVSVAGEATFLIEKQI---MLNKSPNNKELELTKEEFAKVEQVK 188

Query: 82 ETIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQKELMSLDGVGQKTANVV 133
ET+ + +++ + AQKV+ G+ PS + ++++ V + +NVV
Sbjct: 189 ETLMASERALDET---VQEAQKVLNMVNGLNPSNKDQVLAKKDVAKAISNVV 237


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03885FLGMOTORFLIN992e-30 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 99 bits (249), Expect = 2e-30
Identities = 25/77 (32%), Positives = 47/77 (61%)

Query: 34 LICDYKNLLDMEIVFSAELGSTQIPLLQILRFEKGSVIDLQKPAGESVDTFVNGRVIGKG 93
+ D ++D+ + + ELG T++ + ++LR +GSV+ L AGE +D +NG +I +G
Sbjct: 50 AMQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQG 109

Query: 94 EVMVFERNLAIRLNEIL 110
EV+V +R+ +I+
Sbjct: 110 EVVVVADKYGVRITDII 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03895TONBPROTEIN504e-09 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 49.6 bits (118), Expect = 4e-09
Identities = 24/57 (42%), Positives = 28/57 (49%)

Query: 83 APKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPNHKHKALKKVE 139
P P +P P P P P IEKPKP+PKPKPKP K + +K VE
Sbjct: 62 QPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVE 118



Score = 45.4 bits (107), Expect = 9e-08
Identities = 38/228 (16%), Positives = 69/228 (30%), Gaps = 53/228 (23%)

Query: 84 PKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPNHKHKALKKVEKVEE 143
P + P +P P P P P P E P KPKPKP+PK K V+KV+E
Sbjct: 57 PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKP--------KPVKKVQE 108

Query: 144 KKVVEEKKEEKKIVEQKVEQKKVEEKKPVKKEFDPNQLSFLPKEVAPPRQENNKGLDNQT 203
+ + K E + ++ + + +
Sbjct: 109 QPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQPQ------ 162

Query: 204 RRDIDELYGEEFGDLGTAEKDFIRNNLRDIGRITQKYLEYPQVAAYLGQDGTNAVEFYLH 263
YP A L +G V+F +
Sbjct: 163 ---------------------------------------YPARAQALRIEGQVKVKFDVT 183

Query: 264 PNGDITDLKIIIGSEYKMLDDNTLKTIQIAYKDYPRPKTKTLIRIRVR 311
P+G + +++I+ M + ++ + +P + ++ I +
Sbjct: 184 PDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFK 231



Score = 39.6 bits (92), Expect = 8e-06
Identities = 16/54 (29%), Positives = 22/54 (40%)

Query: 74 QDPSKNTPGAPKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKK 127
Q +P P P P+ PKP KPKP+P K + +PK+
Sbjct: 59 QAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKR 112



Score = 38.8 bits (90), Expect = 1e-05
Identities = 25/72 (34%), Positives = 31/72 (43%), Gaps = 1/72 (1%)

Query: 87 TLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPNHKHKALKKVEKVEEKKV 146
T+ P P PP P E P+PEP P+P E K K K + KKV
Sbjct: 48 TMVTPADLEPPQAVQPPPEPVVEPE-PEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKV 106

Query: 147 VEEKKEEKKIVE 158
E+ K + K VE
Sbjct: 107 QEQPKRDVKPVE 118



Score = 33.8 bits (77), Expect = 6e-04
Identities = 14/56 (25%), Positives = 22/56 (39%)

Query: 74 QDPSKNTPGAPKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPN 129
+P P+P P++ P P P PKP K + +PK +P +
Sbjct: 65 PEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESR 120



Score = 31.1 bits (70), Expect = 0.004
Identities = 12/52 (23%), Positives = 16/52 (30%)

Query: 75 DPSKNTPGAPKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPK 126
+P P + P P P P K E+PK + KP
Sbjct: 72 EPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPAS 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_03910TYPE3IMSPROT310.002 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 30.9 bits (70), Expect = 0.002
Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 4/64 (6%)

Query: 88 LQSYSVMLFFNLLLLIDILGFLPFSIYHHFMASLIFSALFCSSLFLSSPLLGVIALVALS 147
L Y F L+L+ +LPFS S + + +L PLL V AL+A++
Sbjct: 45 LSDYYFEHFSKLMLIPAEQSYLPFSQ----ALSYVVDNVLLEFFYLCFPLLTVAALMAIA 100

Query: 148 SSLL 151
S ++
Sbjct: 101 SHVV 104


27HPSAT_04170HPSAT_04200N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_04170212-1.731737cag pathogenicity island protein (cag delta,
HPSAT_04175-212-1.081769cag pathogenicity island protein (cag zeta,
HPSAT_04180-210-0.872801hypothetical protein
HPSAT_04185010-1.798206conserved hypothetical secreted protein
HPSAT_04190010-0.977898GTP-binding protein Era
HPSAT_04195110-1.021944ATP-dependent protease ATP-binding subunit HslU
HPSAT_04200011-0.378352ATP-dependent protease peptidase subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04170PF07201290.035 Hypersensitivity response secretion protein HrpJ
		>PF07201#Hypersensitivity response secretion protein HrpJ

Length = 293

Score = 29.4 bits (66), Expect = 0.035
Identities = 11/53 (20%), Positives = 21/53 (39%), Gaps = 10/53 (18%)

Query: 328 EEREKKLLAEKEKQ--------EAELAKY--KLKDLENQKKLKALEAELKKKN 370
E +E L K E ++ +Y K+ +LE ++ + L + L
Sbjct: 62 ERKELSLDKRKLSDSQARVSDVEEQVNQYLSKVPELEQKQNVSELLSLLSNSP 114


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04175TYPE3IMSPROT270.019 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 27.0 bits (60), Expect = 0.019
Identities = 13/68 (19%), Positives = 24/68 (35%), Gaps = 9/68 (13%)

Query: 27 NLADKRYDSLGLIGAGVLCCVLSGAIGIVGII--FVAIGIFLS-------FSNINLVKLV 77
+ A L+ LC L ++ I V G +S IN ++
Sbjct: 69 SQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKKINPIEGA 128

Query: 78 EKLFKKQS 85
+++F +S
Sbjct: 129 KRIFSIKS 136


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04190PF03944330.001 delta endotoxin
		>PF03944#delta endotoxin

Length = 633

Score = 33.1 bits (75), Expect = 0.001
Identities = 25/94 (26%), Positives = 48/94 (51%), Gaps = 3/94 (3%)

Query: 68 LHHQEKLLNQCMLSQALKAMGDAELCVFLASVHDDLKGYEEFLSLCQKPHILAVSKIDTA 127
L E+ LNQ + + + A +AEL A+V + + + FL+ + L+++
Sbjct: 94 LRETERFLNQRLNTDTV-ARVNAELTGLQANVEEFNRQVDNFLNPNRNAVPLSITSSVNT 152

Query: 128 VHKQVLQKLQEYQQYASQFLALVPLSAKKSQNLN 161
+ + L +L ++Q Q L L+PL A+ + NL+
Sbjct: 153 MQQLFLNRLPQFQMQGYQLL-LLPLFAQAA-NLH 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04195HTHFIS290.045 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.045
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 3/36 (8%)

Query: 51 TPKNILMIGSTGVGKTEIARRI---AKIMKLPFVKV 83
T +++ G +G GK +AR + K PFV +
Sbjct: 159 TDLTLMITGESGTGKELVARALHDYGKRRNGPFVAI 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_04200PF07520290.016 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 28.8 bits (64), Expect = 0.016
Identities = 14/49 (28%), Positives = 23/49 (46%), Gaps = 4/49 (8%)

Query: 121 LEAEDNKIAAIGSGG---NYALSAARALDNFAHLEPRKLVEESLKIAGD 166
E+ ++A I GG + ++ R DN L P + E ++AGD
Sbjct: 590 GESPSLRLACIDVGGGTTDLMVTTYRGEDNRV-LHPEQTFREGFRVAGD 637


28HPSAT_06430HPSAT_06465N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_064300120.745427cation efflux system protein (czcA)
HPSAT_06435111-0.412266hypothetical protein
HPSAT_06440211-0.260964branched-chain amino acid transport protein
HPSAT_06445212-0.185645chaperone protein DnaJ
HPSAT_06450111-0.721379hypothetical protein
HPSAT_06455-111-0.660895tRNA-specific 2-thiouridylase MnmA
HPSAT_06460-213-0.172856hypothetical protein
HPSAT_06465-115-0.407731hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_06430ACRIFLAVINRP8070.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 807 bits (2085), Expect = 0.0
Identities = 225/1062 (21%), Positives = 451/1062 (42%), Gaps = 73/1062 (6%)

Query: 5 IIDLSVKNKLLTTLVTLLIFLASLWAIKSVRLDALPDLSPAQVVVQITYPNQSPKIVQEQ 64
+ + ++ + ++ +++ +A AI + + P ++P V V YP + VQ+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 65 VTYPLVSTFMSIANIDTVRGIS-SYESGLIYIIFKDGVNLYWARDRVLEQLNRALN-LPK 122
VT + I N+ + S S S I + F+ G + A+ +V +L A LP+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 123 DAKV-EIGSDSTSIGWAYQYALSSDSKNLS--DLKVLQDFYYRYALLGVDGVSEVASVGG 179
+ + I + +S + SD+ + D+ + L ++GV +V G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 180 FVKDYEVTLQNDSLIRYNLSLEQVANAIKNSNNDTGGGVI------LENGFEKIIRSHGY 233
+ L D L +Y L+ V N +K N+ G + I +
Sbjct: 181 -QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 234 IQSLKDLEEIVVK-KEGAIPLKIKDIASVRLAPKPRRGAANLNGDKEVVGGIVMVRYHAD 292
++ ++ ++ ++ +++KD+A V L + A +NG K G + + A+
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARING-KPAAGLGIKLATGAN 298

Query: 293 TYKVLKAIKEKIATLQASNP-DVKITSVYDRSELIEKGIDNLIHTLIEESVIVLVIIAIF 351
KAIK K+A LQ P +K+ YD + ++ I ++ TL E ++V +++ +F
Sbjct: 299 ALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLF 358

Query: 352 LLHFRSALVVIITLPLSVCISFLLMRYFNIEASIMSLGGIAIAIGAMVDAAIVMVENAHK 411
L + R+ L+ I +P+ + +F ++ F + +++ G+ +AIG +VD AIV+VEN +
Sbjct: 359 LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVER 418

Query: 412 HLQHIDVKDNAQRVNAIMQGVKHVGGAIFFALMIIVVSFLPIFALTGQEEKLFAPLAYTK 471
+ +D A + + + GA+ M++ F+P+ G ++ + T
Sbjct: 419 VMM----EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITI 474

Query: 472 TFAMLVGALLSITMVPILMVWLIKGRILEESKNPINAFF----------MKIYGVSLNVV 521
AM + L+++ + P L L+K E +N FF + Y S+ +
Sbjct: 475 VSAMALSVLVALILTPALCATLLKPVSAEHHENK-GGFFGWFNTTFDHSVNHYTNSVGKI 533

Query: 522 LKFRYAFLIASVVGLGGLYVAYQKLNWEFIPQINEGVVMYMPVTINGVGID-------TA 574
L +L+ + + G+ V + +L F+P+ ++GV + M G +
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 575 LEYLKKSNSAIKRLDFVKQVFGKVGRANTSTDAAGLAMIETYIELKPQNEWKEKLSYKEV 634
+Y K+ A F F G+A + A ++ LKP W+E+ +
Sbjct: 594 TDYYLKNEKANVESVFTVNGFSFSGQAQNAGMA--------FVSLKP---WEERNGDENS 642

Query: 635 RDKL--EKTLQLKGLTNSWTYPIRGRTDMLLTGIRTPLGIKL-------YGNDTDKLQEL 685
+ + ++L + + + P + L G T +L + T +L
Sbjct: 643 AEAVIHRAKMELGKIRDGFVIPFNMPAIVEL-GTATGFDFELIDQAGLGHDALTQARNQL 701

Query: 686 AILMEQQLKTLKESLSVFAERSNNGYYITLDLNDENLARYGINKNAVLDTIKFALGGTTL 745
+ Q +L +SV + L+++ E G++ + + TI ALGGT +
Sbjct: 702 LGMAAQHPASL---VSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYV 758

Query: 746 TTMIKGVENYPISLRLEDTERNTIERLQNLYVKTAYNYM-PLRELAHVYYDNSPAVLKSE 804
I + ++ + R E + LYV++A M P ++ L+
Sbjct: 759 NDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERY 818

Query: 805 KGLNVNFIYIVPQANISSDTYRQLAQKALEKIKLPSGYYYEFSGESQYLEEAFKTLQYIV 864
GL I SS L + KLP+G Y+++G S + +V
Sbjct: 819 NGLPSMEIQGEAAPGTSSGDAMALMENLAS--KLPAGIGYDWTGMSYQERLSGNQAPALV 876

Query: 865 PVSVFIIFILIVFALKNLTNSLLCFFTLPFAFLGGLIFMNIMGFNMSVAALVGFLALLGV 924
+S ++F+ + ++ + + +P +G L+ + V +VG L +G+
Sbjct: 877 AISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGL 936

Query: 925 ASETAIVMIIYLEDAFQKFIKTPLKEQNSTALKEAIMHGAVLRVRPKLMTFFSILASLIP 984
+++ AI+++ + +D L E+ + EA + +R+RP LMT + + ++P
Sbjct: 937 SAKNAILIVEFAKD---------LMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLP 987

Query: 985 IMYSHGTGSEIMKSIAAPMLGGMISSVVLTLFIIPTAYFVIK 1026
+ S+G GS ++ ++GGM+S+ +L +F +P + VI+
Sbjct: 988 LAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_06440TCRTETB280.035 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 27.9 bits (62), Expect = 0.035
Identities = 17/88 (19%), Positives = 41/88 (46%), Gaps = 6/88 (6%)

Query: 144 FGSLVGSLVGTHFSFD---TQGMEFVMTAIFIVLFMEQYKRNTNHKN--AWLGIFIAVVC 198
G +G ++ + + M ++T F++ +++ R H + + + + +V
Sbjct: 154 VGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVF 213

Query: 199 LALFGTEYFLLIALVLMVLALILFKKQL 226
LF T Y + L++ VL+ ++F K +
Sbjct: 214 FMLFTTSYSISF-LIVSVLSFLIFVKHI 240


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_06450cloacin330.003 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 32.8 bits (74), Expect = 0.003
Identities = 21/99 (21%), Positives = 47/99 (47%), Gaps = 9/99 (9%)

Query: 28 AKLSRSNEQLSDMLYKLNESLRIYQSVLSNNQDQL----KEIKKANSTLNSQRRFFNASQ 83
++L +N+ L+D + ++ + R ++ + ++A + +N+++ F+A+
Sbjct: 356 SELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAA 415

Query: 84 IRLMDTDALLKQSALELEKLQALEKRLKEGMEQERLIEE 122
D DA L SA+E K +K K+ + L +E
Sbjct: 416 KEKSDADAAL-SSAMESRK----KKEDKKRSAENNLNDE 449


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_06465LPSBIOSNTHSS473e-09 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 47.1 bits (112), Expect = 3e-09
Identities = 24/71 (33%), Positives = 39/71 (54%), Gaps = 4/71 (5%)

Query: 11 ALYGGSFDPLHKAHLAIIDQTLELLPFAKLIVLPAYQNPFKKPCFLDAQTRFKELERALK 70
A+Y GSFDP+ HL II++ L F ++ V +NP K+P F Q R +++ +A+
Sbjct: 3 AIYPGSFDPITFGHLDIIERGCRL--FDQVYVAVL-RNPNKQPMF-SVQERLEQIAKAIA 58

Query: 71 GMDRVLLSDFE 81
+ + FE
Sbjct: 59 HLPNAQVDSFE 69


29HPSAT_07630HPSAT_07670N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPSAT_07630-1131.581788flagellar hook-basal body protein FliE
HPSAT_07635-1111.489047flagellar basal body rod protein FlgC
HPSAT_07640-1121.088627flagellar basal body rod protein FlgB
HPSAT_076450121.410937probable cell division protein ftsW
HPSAT_076500120.074040iron(III) ABC transporter, periplasmic
HPSAT_07655013-0.007463hypothetical protein
HPSAT_076601140.333666putative peroxidase
HPSAT_07665112-0.181342outer membrane protein
HPSAT_07670213-0.337105penicillin-binding protein 2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07630FLGHOOKFLIE776e-22 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 77.0 bits (189), Expect = 6e-22
Identities = 19/77 (24%), Positives = 40/77 (51%), Gaps = 1/77 (1%)

Query: 34 EQKGGEFSKLLKQSINELNNTQEQSDKALADMATGQIK-DLHQAAIAIGKAETSMKLMLE 92
Q F+ L +++ +++TQ + G+ L+ + KA SM++ ++
Sbjct: 27 PQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQ 86

Query: 93 VRNKAISAYKELLRTQI 109
VRNK ++AY+E++ Q+
Sbjct: 87 VRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07635FLGHOOKAP1280.013 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 28.4 bits (63), Expect = 0.013
Identities = 10/38 (26%), Positives = 15/38 (39%)

Query: 121 NVNAVVEMADLVEATRAYQANVAAFQSAKNMAQNAIGM 158
VN E +L + Y AN Q+A + I +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07650FERRIBNDNGPP362e-04 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 35.7 bits (82), Expect = 2e-04
Identities = 30/183 (16%), Positives = 78/183 (42%), Gaps = 10/183 (5%)

Query: 108 NVELLKKLSPDLVVTFVG-NPKAVEHAKKFGISFLSFQETT--IAEAMQAMQ--AQATVL 162
N+ELL ++ P +V G P A+ +F + +A A +++ A L
Sbjct: 88 NLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNL 147

Query: 163 EIDASKKFAKMQETLDFIKERL-KDVKKKKGVELFHKAN--KISGHQAISSDILEKGGID 219
+ A A+ ++ + +K R K + + + G ++ +IL++ GI
Sbjct: 148 QSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIP 207

Query: 220 N-FGLKYVKFGRADVSVEKIVK-ENPEIIFIWWVSPLTPEDVLNNPKFATIKAIKNKQVY 277
N + + +G VS++++ ++ +++ + + ++ P + + ++ +
Sbjct: 208 NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQ 267

Query: 278 KLP 280
++P
Sbjct: 268 RVP 270


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07655FERRIBNDNGPP355e-04 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 34.5 bits (79), Expect = 5e-04
Identities = 31/183 (16%), Positives = 74/183 (40%), Gaps = 10/183 (5%)

Query: 106 NVELLKKLSPDLVVTFVGNPKAVEHAKKF--GISFLSFQEKTIAEVMEDID---AQAKAL 160
N+ELL ++ P +V G + E + G F K + A L
Sbjct: 88 NLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNL 147

Query: 161 EIDASKKLAKMQETLDFIKERL-KDVKKKKGVELFHKAN--KISGHQALDSDILEKGGID 217
+ A LA+ ++ + +K R K + + + G +L +IL++ GI
Sbjct: 148 QSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIP 207

Query: 218 N-FGLKYVKFGRADVSVEKIVK-ENPEIIFIWWVSPLSPEDVLNNPKFATIKAIKNKQVY 275
N + + +G VS++++ ++ +++ + + ++ P + + ++ +
Sbjct: 208 NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQ 267

Query: 276 KLP 278
++P
Sbjct: 268 RVP 270


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPSAT_07670TYPE3IMPPROT290.030 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 29.4 bits (66), Expect = 0.030
Identities = 9/23 (39%), Positives = 12/23 (52%)

Query: 4 LRYKLLLFVFIGFWGLLVLNLFI 26
KL+LFV + W LL L +
Sbjct: 195 TPIKLVLFVALDGWTLLSKGLIL 217



 
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