PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genome393.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_009504 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1BOV_A0025BOV_A0041Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A00250143.198416branched-chain amino acid ABC transporter
BOV_A00260143.747758glyoxalase family protein
BOV_A00270142.818524putative 3-oxoacyl-(acyl-carrier-protein)
BOV_A00281143.001770short chain dehydrogenase/reductase family
BOV_A00290122.538968putative acetoin dehydrogenase, alpha/subunit
BOV_A00310131.586930shikimate dehydrogenase
BOV_A00322151.110842hypothetical protein
BOV_A00332140.310590magnesium-translocating P-type ATPase
BOV_A0034017-0.874109hypothetical protein
BOV_A0035017-1.522279MgtC family protein
BOV_A0036015-1.603723hypothetical protein
BOV_A0037-115-0.464349sensor histidine kinase
BOV_A0038-1140.154397LuxR family DNA-binding response regulator
BOV_A00401161.143098*response regulator
BOV_A00412171.083627hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0027DHBDHDRGNASE1169e-34 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 116 bits (292), Expect = 9e-34
Identities = 81/250 (32%), Positives = 115/250 (46%), Gaps = 14/250 (5%)

Query: 6 ENRTLVLTGANGGIGRAIAELFHASGANLVLTDLDREGLDAFAASLGSPERIA-TIKADA 64
E + +TGA GIG A+A + GA++ D + E L+ +SL + R A AD
Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADV 66

Query: 65 SSADDAEKTVALAMERFGGIDFLVPSAGIYQAKPFAEMSDADWHRTISINLDGVFYLCKR 124
+ ++ A G ID LV AG+ + +SD +W T S+N GVF +
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 125 ALPALK--EDSSIVTLASLAAYRGAYVNAHYGATKGAMVSMTRALSRELAP-KTRVNGVA 181
+ SIVT+ S A A Y ++K A V T+ L ELA R N V+
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 182 PGIIETPMTSEL----------LKTRMDETMTQTPLKRLGKPSEIASVIAFLCSPAASFV 231
PG ET M L +K ++ T PLK+L KPS+IA + FL S A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 232 TGETIQVNGG 241
T + V+GG
Sbjct: 247 TMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0028DHBDHDRGNASE1175e-34 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 117 bits (294), Expect = 5e-34
Identities = 76/250 (30%), Positives = 119/250 (47%), Gaps = 12/250 (4%)

Query: 7 KLVLVTGAGRGLGAAISSGAAEQGARVILVDIDGTAAKAQADALTAKGFVAEGHALDVTD 66
K+ +TGA +G+G A++ A QGA + VD + + +L A+ AE DV D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 67 RDAVAALADDILSRFGGLDVLVNNAGVAGRAAFDQPEAVEVWDRVIGVNLEGAFNVSHAL 126
A+ + I G +D+LVN AGV + E W+ VN G FN S ++
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHS-LSDEEWEATFSVNSTGVFNASRSV 127

Query: 127 VPALKAAK-GNVVHLCSVAGFVSGGSTAGYVVSKGAIRSLTQVMARDLAPHGIRVNAVAP 185
+ + G++V + S V S A Y SK A T+ + +LA + IR N V+P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 186 GIMMSEM---------AVAQLNRPGGTDWFMNRVMMKRIGETSEVVDPVVFLASPMASYI 236
G ++M Q+ + G + F + +K++ + S++ D V+FL S A +I
Sbjct: 188 GSTETDMQWSLWADENGAEQVIK-GSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 237 TGTILPVDGG 246
T L VDGG
Sbjct: 247 TMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0038HTHFIS1072e-29 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 107 bits (269), Expect = 2e-29
Identities = 28/123 (22%), Positives = 55/123 (44%)

Query: 11 VFIVDDDHSVRLGLVDLFASIGLKALCFASVNEFLKHAREEVPACLILDVRMPGESGTEF 70
+ + DDD ++R L + G ++ + ++ DV MP E+ +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 71 HARMKGLDIRLPVIFITGHGDIAMGVKAIKDGAIDFLAKPFRNQDLLDAVQQAIRTDRKR 130
R+K LPV+ ++ +KA + GA D+L KPF +L+ + +A+ ++R
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 131 LRE 133
+
Sbjct: 126 PSK 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0040HTHFIS925e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 91.8 bits (228), Expect = 5e-25
Identities = 34/115 (29%), Positives = 57/115 (49%)

Query: 3 RILLAEDDNDMRRFLVKALEKAGYHVTHFDNGASAYERLQEEPFSLLLTDIVMPEMDGIE 62
IL+A+DD +R L +AL +AGY V N A+ + + L++TD+VMP+ + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 LARRATEIDPDLKIMFITGFAAVALNPDSDAPRDAKVLSKPFHLRDLVNEIEKML 117
L R + PDL ++ ++ + L KPF L +L+ I + L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119


2BOV_A0052BOV_A0059Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0052321-0.214254hypothetical protein
BOV_A0053520-0.871601putative lipoprotein
BOV_A0054419-1.112843P-type DNA transfer ATPase VirB11
BOV_A0055419-1.884229type IV secretion system protein VirB10
BOV_A0056320-2.372070P-type conjugative transfer protein VirB9
BOV_A0057421-2.617514type IV secretion system protein VirB8
BOV_A0058421-2.482511type IV secretion system protein VirB6
BOV_A0059219-1.743798P-type DNA transfer protein VirB5
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0053OMPADOMAIN512e-10 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 51.5 bits (123), Expect = 2e-10
Identities = 32/144 (22%), Positives = 56/144 (38%), Gaps = 21/144 (14%)

Query: 47 FPQEPTAQATMWPARPPKQTVS--------VYFPQDVTVFRPTSAQ-INQLHTLLWPV-P 96
F Q A P + + V F + +P ++QL++ L + P
Sbjct: 191 FGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDP 250

Query: 97 KH--INVRGLTDNNCPPPGDTQVARVRALAIYNWLINQGVPASRI-TISYAPVKDYASN- 152
K + V G TD + ++ RA ++ ++LI++G+PA +I N
Sbjct: 251 KDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNT 310

Query: 153 -------APLSPGRVLNRRVDIEI 169
A L +RRV+IE+
Sbjct: 311 CDNVKQRAALIDCLAPDRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0055PF03544391e-05 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 39.2 bits (91), Expect = 1e-05
Identities = 26/122 (21%), Positives = 33/122 (27%), Gaps = 1/122 (0%)

Query: 33 VLLFLFVVGFIVVLLLLLVFHMRGN-AENNHHSDKTMVQTSTVPMRTFKLPPPPPPAPPE 91
LL + + G +V LL H TMV + + PPP P PE
Sbjct: 18 TLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPE 77

Query: 92 PPAPPPAPAMPIAEPAAAALSLPPLPDDTPAKDDVLDKSASALMVVTKSSGDTNAQTTNA 151
P P A P P P K K + +S N
Sbjct: 78 PEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARP 137

Query: 152 RI 153

Sbjct: 138 TS 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0056TYPE4SSCAGX512e-09 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 50.9 bits (121), Expect = 2e-09
Identities = 47/197 (23%), Positives = 72/197 (36%), Gaps = 58/197 (29%)

Query: 93 SHSNQSIDMAPEPGKWDTNLMVTTDQRMYDFDLRLMPGRNNQRVAYRVQFRYPAAAAAAA 152
S + SI+++P W TNL+V T++ +Y F LR+ N V+ YP ++
Sbjct: 262 SPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAYLTVKLEYPQRHEVSS 321

Query: 153 V----------AAAQKRVV-QARMNA---------------------------------- 167
V A Q+ ++ Q +N
Sbjct: 322 VIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKEKIREEKQKIILDQAK 381

Query: 168 -----------RPSPVNWNYTMQVG--TNSASIAPTLAYDDGRFTYLRFPNNRDFPAAFL 214
+ +PV NY S I P+ +DDG FTY F N PA F+
Sbjct: 382 ALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQPAIFV 441

Query: 215 VAEDKSESIVNSHIDPS 231
V D S+ ++ IDP+
Sbjct: 442 VQPDGKLSMTDAAIDPN 458


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0057PF043352156e-73 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 215 bits (550), Expect = 6e-73
Identities = 58/217 (26%), Positives = 104/217 (47%), Gaps = 7/217 (3%)

Query: 23 YDEALNWEAAHVRLIEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNAQT 82
++EA +WE + E+S++ AW +AG G + + +A + PLK PY++ V+ T
Sbjct: 14 FEEAASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNT 73

Query: 83 GAPDILTSLD-EKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSY 141
G I L + +++YD + KY+L+ YV RE + ++ ++ V ++S+ E +
Sbjct: 74 GEASIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRW 133

Query: 142 ASQFQGD--KALDKQYGSNVRTSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWI 199
+ ++ D ++ + V I + G + V F K + + + T +
Sbjct: 134 SRFYKTDNPQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNS---TKTDAV 190

Query: 200 ATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMG 236
ATI Y+ E R NPLG+ V SYR D E+
Sbjct: 191 ATIKYKVDGTP-SKEVDRFKNPLGYQVESYRADVEVP 226


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0058CHANLCOLICIN320.004 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 32.0 bits (72), Expect = 0.004
Identities = 16/31 (51%), Positives = 19/31 (61%), Gaps = 2/31 (6%)

Query: 305 SGSSGGGGSGSAKAGGESSYSAGGNAMWSPA 335
SGS GGGG G +K+ ESS + A WS A
Sbjct: 32 SGSGGGGGKGGSKS--ESSAAIHATAKWSTA 60


3BOV_A0078BOV_A0083Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A00782142.318203hypothetical protein
BOV_A00793160.533780keto-hydroxyglutarate-aldolase/keto-deoxy-
BOV_A0080317-0.616792hypothetical protein
BOV_A0081314-0.383660rhodanese family protein
BOV_A0082415-1.047994molybdate ABC transporter substrate-binding
BOV_A00834150.012317molybdate ABC transporter permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0078IGASERPTASE364e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 36.2 bits (83), Expect = 4e-04
Identities = 41/288 (14%), Positives = 80/288 (27%), Gaps = 32/288 (11%)

Query: 269 ADEEEDNGDTAAPATARPARATAPTRQTPAAVPAAPVEQPRETMVAALPARDAP-VPMQA 327
++ DT T +A P+ + A E P A P+ V +
Sbjct: 985 VEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENS 1044

Query: 328 PRPETGLDAQAAAAETPVAAFNVPVPAHKPQMAPQDAVALAAAEDPAAAARAAVDANTAQ 387
+ ++ A A ++A + + A A++ + Q
Sbjct: 1045 KQESKTVEKNEQDATETTA--------QNREVAKEAKSNVKANTQTNEVAQSGSETKETQ 1096

Query: 388 VADAGMSAALTNGFVPVPSARPHLETDNVQLAAAVIPTARP-------ERPGEHPARGQD 440
+ +A + + +ET+ Q V P +P PAR D
Sbjct: 1097 TTETKETATVEK------EEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREND 1150

Query: 441 AIAALVNSQSDEPAQMAGEMADATGNAVAPQPGKTAAFPLPTQAPRADTKLAMVSRRDEI 500
+ QS E ++ QP + + + +
Sbjct: 1151 PTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTEST---TVNTGNSVVENPENTTPAT- 1206

Query: 501 GELIAPPEDDYDYDAKPVA--AAAPKQAPAPVQMASAAPTKPAAVASA 546
P + + KP + + P V+ A+ + + VA
Sbjct: 1207 ----TQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALC 1250


4BOV_A0145BOV_A0152Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0145221-0.531877hypothetical protein
BOV_A0146324-0.963014LysR family transcriptional regulator
BOV_A0147523-0.773321CAIB/BAIF family protein
BOV_A0148524-1.426704hypothetical protein
BOV_A0149426-2.080382putative citrate lyase subunit beta
BOV_A0150627-2.804883hypothetical protein
BOV_A0151421-1.975005putative cytochrome b561
BOV_A0152219-0.434345outermembrane transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0152IGASERPTASE320.013 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.0 bits (72), Expect = 0.013
Identities = 39/149 (26%), Positives = 49/149 (32%), Gaps = 13/149 (8%)

Query: 433 SGVNTYTGGTTLTAGTLAAASDNNLGG------ASGGLTFNGGTLQVMGTSWTSTNWAVS 486
+T TGG LA D G SG LT N Q G + ++ V
Sbjct: 355 GKTSTITGGEKSLNVDLADGKDKPNHGKSVTFEGSGTLTLNNNIDQGAGGLFFEGDYEVK 414

Query: 487 LQAGGGTFDIE--DAANNFAVTQGVAGAGG--LTKSGSGTLTLSGANSYTGATTVSAGTL 542
+ T+ A VT V L K G GTL + G G+ V GT+
Sbjct: 415 GTSDNTTWKGAGVSVAEGKTVTWKVHNPQYDRLAKIGKGTLIVEGTGDNKGSLKVGDGTV 474

Query: 543 ---TQGAAGGFSTASSRYDVDTDGTLDLG 568
Q G +S V TL L
Sbjct: 475 ILKQQTNGSGQHAFASVGIVSGRSTLVLN 503


5BOV_A0171BOV_A0177Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A01715250.943871ATP phosphoribosyltransferase catalytic subunit
BOV_A01736310.725174hypothetical protein
BOV_A01747261.075644fumarate hydratase
BOV_A01755230.495434hypothetical protein
BOV_A01765220.959386hypothetical protein
BOV_A01773190.446610chaperonin GroEL
6BOV_A0218BOV_A0226Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A02182121.778053dihydrodipicolinate synthase family protein
BOV_A02193132.014301malate/L-lactate dehydrogenase family protein
BOV_A02202121.300152hypothetical protein
BOV_A02211133.169962hypothetical protein
BOV_A02220113.056133cytochrome c oxidase subunit III
BOV_A0223-1133.466810norF protein
BOV_A0224-1133.134655nitric-oxide reductase, small subunit
BOV_A0226-1143.003483CbbQ/NirQ/NorQ/GpvN family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0226HTHFIS320.003 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.7 bits (72), Expect = 0.003
Identities = 26/97 (26%), Positives = 39/97 (40%), Gaps = 13/97 (13%)

Query: 25 CALFEMAWQRKLPLLLKGPTGCGKT---RFVSHMAARMGLPLSTVSCH---DDLAAADLT 78
+ Q L L++ G +G GK R + R P ++ DL ++L
Sbjct: 150 YRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELF 209

Query: 79 GRYLLKG---GDTIWMDGRLTRAVRDGGICYLDEIVE 112
G KG G GR +A +GG +LDEI +
Sbjct: 210 GHE--KGAFTGAQTRSTGRFEQA--EGGTLFLDEIGD 242


7BOV_A0288BOV_A0293Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0288112-4.485492hypothetical protein
BOV_A0289112-3.336938hypothetical protein
BOV_A0290211-3.530410glutaredoxin-like protein nrdH
BOV_A0291212-3.672406ribonucleotide reductase stimulatory protein
BOV_A0292212-3.625671ribonucleotide-diphosphate reductase subunit
BOV_A0293211-2.295757ribonucleotide-diphosphate reductase subunit
8BOV_A0466BOV_A0522Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0466-113-3.181858oligopeptide ABC transporter permease
BOV_A0467013-3.994836oligopeptide ABC transporter substrate-binding
BOV_A0468014-3.122756oligopeptide ABC transporter substrate-binding
BOV_A0469317-0.822724transposase OrfA
BOV_A04702170.905571transposase OrfB
BOV_A04713181.938898hypothetical protein
BOV_A04724171.601503hypothetical protein
BOV_A04733161.965188NAD-dependent epimerase/dehydratase family
BOV_A04742182.413657UDP-glucose 4-epimerase
BOV_A04760152.191434UDP-glucose 6-dehydrogenase
BOV_A04770160.363384hypothetical protein
BOV_A0478-118-0.109932endoglucanase family protein
BOV_A0479022-1.144715hypothetical protein
BOV_A0480127-2.580604hypothetical protein
BOV_A0481026-1.897229AraC family transcriptional regulator
BOV_A0482028-3.049366IS711, transposase orfB
BOV_A0483128-2.525485IS711, transposase orfA
BOV_A0484128-2.952112TRm24 transposase
BOV_A0487324-1.921626hypothetical protein
BOV_A0488326-0.946232AraC family transcriptional regulator
BOV_A0489225-1.988460transposase
BOV_A0490326-2.204857transposase
BOV_A0491325-2.198665hypothetical protein
BOV_A0492425-3.371535hypothetical protein
BOV_A0494325-3.728254hypothetical protein
BOV_A0495325-4.107861Y4jO-like protein
BOV_A0496430-4.922491hypothetical protein
BOV_A0497429-6.299237putative prevent-host-death protein
BOV_A0500430-6.510831ABC transporter ATP-binding protein
BOV_A0501430-6.423687ABC transporter ATP-binding protein
BOV_A0502430-6.680915ABC transporter permease
BOV_A0503330-6.669707ABC transporter permease
BOV_A0504329-6.365983ABC transporter substrate-binding protein
BOV_A0505431-4.609784amidohydrolase
BOV_A0507623-0.710582hypothetical protein
BOV_A0506526-3.259257hypothetical protein
BOV_A0508528-3.770334hypothetical protein
BOV_A0510428-3.712406transposase, Tn3 family
BOV_A0511529-4.077824hypothetical protein
BOV_A0513428-3.966229transposase
BOV_A0512534-5.635688putative YapH protein
BOV_A0514634-4.927618transposase OrfB
BOV_A0515530-3.836504transposase OrfA
BOV_A0516526-3.094017hemagglutinin
BOV_A0517425-2.535546transposase OrfA
BOV_A0519524-2.170443transposase OrfB
BOV_A0518422-1.584364putative cell wall surface protein
BOV_A0521322-0.654539IS66 family element, orf4
BOV_A0522219-1.316191IS66 family element, orf3
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0473NUCEPIMERASE2028e-65 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 202 bits (515), Expect = 8e-65
Identities = 83/337 (24%), Positives = 138/337 (40%), Gaps = 41/337 (12%)

Query: 20 VLVAGGAGFLGSHLCERLLNEGHSVICVDNFSTGRIENIRH----LLNFDGFSFIRHDIV 75
LV G AGF+G H+ +RLL GH V+ +DN + +++ LL GF F + D+
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDLA 62

Query: 76 NTLDLR-------VDEIYNLACPASPPHYQADPVHTMKTNVIGSLNLLELAAHYK-ARIF 127
+ + + ++ + + +P +N+ G LN+LE H K +
Sbjct: 63 DREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHLL 122

Query: 128 QASTSEVYGDPHVHPQPENYWGNVNSFGPRSCYDEGKRSAETLFHDFHQQYGVDIRIVRI 187
AS+S VYG P + +V+ P S Y K++ E + H + YG+ +R
Sbjct: 123 YASSSSVYGLNRKMPFSTD--DSVDH--PVSLYAATKKANELMAHTYSHLYGLPATGLRF 178

Query: 188 FNTYGPRMRPDDGRVVSNFIVQALKGEDITVYGDGSQTRSFCYVDDLIEGFHRLM----- 242
F YGP RPD + F L+G+ I VY G R F Y+DD+ E RL
Sbjct: 179 FTVYGPWGRPD--MALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPH 236

Query: 243 ---------------YSPHAIRVPVNIGNPGEFTVGALAEQIIAMTGSRSRIVYYPLPVD 287
+P+ + NIGN + + + G ++ PL
Sbjct: 237 ADTQWTVETGTPAASIAPYRV---YNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPG 293

Query: 288 DPRQRRPDITVAKRELGWEPTVALAQGLEPTIAYFEH 324
D + D +G+ P + G++ + ++
Sbjct: 294 DVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRD 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0474NUCEPIMERASE1505e-45 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 150 bits (381), Expect = 5e-45
Identities = 76/326 (23%), Positives = 131/326 (40%), Gaps = 42/326 (12%)

Query: 5 NVLVVGGAGFIGSHTAKLLAGQGYAPVVYDNLSTGHQSAVRWG----------DFVEGDI 54
LV G AGFIG H +K L G+ V DNL+ + +++ F + D+
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 LDQARLVETMEKYVPVAVIHFAASAYVGESVEDPAKYYRNNVGGTQSLLDACRLTRTQNV 114
D+ + + V V S+E+P Y +N+ G ++L+ CR + Q++
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 115 IFSSSCATYGVPSRLPIGE-GEAQNPINPYGRTKLIAEHMLADYAVAYGLRYVALRYFNA 173
+++SS + YG+ ++P +P++ Y TK E M Y+ YGL LR+F
Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFT- 180

Query: 174 SGADIDGELGEKHDPETHLIPRAMMAAAGRLDVLEVYGDDYETPDGTCIRDYIHVTDLAR 233
+ G G + D +AM+ ++VY G RD+ ++ D+A
Sbjct: 181 ----VYGPWG-RPDMALFKFTKAMLEG----KSIDVYN------YGKMKRDFTYIDDIAE 225

Query: 234 AHVLAVEHLKEAGGNLAV---------------NLGTGRGTSIREIVQSIGRLTGRSVPV 278
A + + + A V N+G + + +Q++ G
Sbjct: 226 AIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKK 285

Query: 279 AMRARRAGDPPALYADPALAAEKLGF 304
M + GD AD E +GF
Sbjct: 286 NMLPLQPGDVLETSADTKALYEVIGF 311


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0478SALSPVBPROT290.026 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 28.9 bits (64), Expect = 0.026
Identities = 20/58 (34%), Positives = 22/58 (37%), Gaps = 1/58 (1%)

Query: 226 LEVGRDTTFVERTKPGYDRVARFNKPIAIAELGYEGDDDYVRAWVAEANKPHAEFPAM 283
E+G T V R YD + A L YEG D Y RA V P P M
Sbjct: 319 DELGEADTLVSRLLLEYDENPILTQLCAARTLAYEG-DGYRRAPVNNMMPPPPPPPMM 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0488SECA342e-04 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 34.5 bits (79), Expect = 2e-04
Identities = 14/43 (32%), Positives = 19/43 (44%), Gaps = 4/43 (9%)

Query: 3 PHPSLSAPKIRPDLIRPVETMGPYDSCWCRSGKKYKWCHFRRE 45
+ +A + + G D C C SGKKYK CH R +
Sbjct: 863 DSAAAAALAAQTGERKV----GRNDPCPCGSGKKYKQCHGRLQ 901


9BOV_A0576BOV_A0585Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0576212-1.362156polar differentiation response regulator
BOV_A0577212-0.776124hypothetical protein
BOV_A0578212-1.039070nucleoside diphosphate kinase regulator
BOV_A0579214-1.879201DNA polymerase IV
BOV_A0581216-3.733500hypothetical protein
BOV_A0580625-4.736537hypothetical protein
BOV_A0583326-4.488990*endonuclease V
BOV_A0584229-4.876804hypothetical protein
BOV_A0586130-5.569902hypothetical protein
BOV_A0585220-2.145878hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0576HTHFIS643e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.1 bits (156), Expect = 3e-15
Identities = 20/119 (16%), Positives = 50/119 (42%), Gaps = 5/119 (4%)

Query: 12 MTK-SVMIVEDNELNMKLFRDLIEASGYETIRTRSGLEALDLAREHHPDLILMDIQLPEV 70
MT ++++ +D+ + + +GY+ T + DL++ D+ +P+
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 71 SGLEVTKWLKDDEELRHIPVIAVTAFAMKGDE-ERIRQGGCEAYISKPISVPRFIETIK 128
+ ++ +K + +PV+ ++A + +G + Y+ KP + I I
Sbjct: 61 NAFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYD-YLPKPFDLTELIGIIG 116


10BOV_A0752BOV_A0758Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0752314-1.922874nickel transporter permease NikC
BOV_A0753216-2.824118nickel transporter permease NikB
BOV_A0754218-3.588405nickel ABC transporter substrate-binding
BOV_A0755320-3.557374nickel responsive regulator
BOV_A0756320-3.343012UxaA family hydrolase
BOV_A0757223-3.436651ABC transporter permease
BOV_A0758023-3.004943ABC transporter permease
11BOV_A0781BOV_A0790Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0781328-1.571124*hypothetical protein
BOV_A0782227-0.393663hypothetical protein
BOV_A0783419-0.740340hypothetical protein
BOV_A0784423-2.278001hypothetical protein
BOV_A0785520-1.014532hypothetical protein
BOV_A0786519-0.978659hypothetical protein
BOV_A0787519-0.957547hypothetical protein
BOV_A0788420-1.117892type I restriction-modification system, M
BOV_A0789422-0.928552type I restriction-modification system subunit
BOV_A0790418-0.198821type I restriction-modification enzyme, R
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0788FLGMOTORFLIM300.017 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 30.2 bits (68), Expect = 0.017
Identities = 11/43 (25%), Positives = 19/43 (44%)

Query: 6 LATTRIIEVNMAKLTRTELENHLWKSADILRGSIDSSDYKIYI 48
+ T ++ A+LT T L L + S+D Y+ +I
Sbjct: 52 MRTLSLMHETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFI 94


12BOV_A1004BOV_A1015Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A1004-111-3.362227ubiquinone/menaquinone biosynthesis
BOV_A1005116-4.368010uracil phosphoribosyltransferase
BOV_A1006227-5.855927hypothetical protein
BOV_A1007231-6.620183methionine sulfoxide reductase A
BOV_A1008336-8.178936hypothetical protein
BOV_A1010134-6.682640*IS3 family transposase orfB
BOV_A1011030-5.280385IS3 family transposase orfA
BOV_A1014-122-3.897711IS3 family transposase orfA
BOV_A1015-121-3.040274IS3 family transposase orfB
13BOV_A1047BOV_A1053Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A1047315-0.303064motD-related protein
BOV_A1048315-0.737550chemotaxis protein
BOV_A1050416-1.058703hypothetical protein
BOV_A1051416-1.201903flagellar MS-ring protein
BOV_A1052517-1.299951flagellin family protein
BOV_A1053516-1.007896outer membrane autotransporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1047FLGHOOKFLIK347e-04 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 34.4 bits (78), Expect = 7e-04
Identities = 28/129 (21%), Positives = 52/129 (40%), Gaps = 15/129 (11%)

Query: 257 KTLQIRLDPVELGTVTARIRVAGDSVEAHLVADKSHAAEMLAADRSMIEKALKVAGVGDD 316
++ ++RL P +LG V ++V + + +V+ H L A ++ L +G+
Sbjct: 257 QSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGI--- 313

Query: 317 TKISVTVADRNAQGAAQHVAAAQNAGQQQASAQQQGHQLAFNMQQQGSQGRGGEAQAQFM 376
Q +++ +GQQQA++QQQ Q N + + +
Sbjct: 314 ------------QLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSL 361

Query: 377 SGRSGGEGG 385
GR G G
Sbjct: 362 QGRVTGNSG 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1051FLGMRINGFLIF5380.0 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 538 bits (1386), Expect = 0.0
Identities = 159/576 (27%), Positives = 252/576 (43%), Gaps = 76/576 (13%)

Query: 9 LGKLGARKLIALGLVGAALMGAILYTSIYLGRPSYETLYVGLSRDDVNRMGLALGEAGIP 68
L +L A I L + G+A + ++ ++ P Y TL+ LS D + L + IP
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 69 FDVKSDGSSILVPIGKAENARMYLAEKGLPTSNNAGYELFDNMGSLGLTSFMQEITRVRA 128
+ + +I VP K R+ LA++GLP G+EL D G++ F +++ RA
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQE-KFGISQFSEQVNYQRA 134

Query: 129 LEGEIARTIQAIRGVKAARVHIVLAEKGSFRRGDQKPSASVVIRAEGGFSAESAQ--SIR 186
LEGE+ARTI+ + VK+ARVH+ + + F R + PSASV + E G + + Q ++
Sbjct: 135 LEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVV 194

Query: 187 QLVAAAVPSLDASSVTVLDTNGHLLA---SAGEGANGAALMTASLEQQVASHVDDSIRKA 243
LV++AV L +VT++D +GHLL ++G N A L A+ V S + I
Sbjct: 195 HLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAN---DVESRIQRRIEAI 251

Query: 244 LAPYLGLGHFQTSVQAALDTDRRQTKETTYDPESRVERSVRVVRESGDSRNNRNDNATGV 303
L+P +G G+ V A LD ++ E Y P ++ R+ S GV
Sbjct: 252 LSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGV 311

Query: 304 -------------------------EQNIPQEQIQNRNGESSTEKTDRREELTNYEVNSK 338
QN PQ N S+ ++ +R E +NYEV+
Sbjct: 312 PGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTST-NSNSAGPRSTQRNETSNYEVDRT 370

Query: 339 TVSTVSDGYSIKRLSIAVVIDQARLLQTAGTTPPPANFVDQQITKIRDLVATAAGLNTNR 398
T + I+RLS+AVV++ L A P P Q+ +I DL A G + R
Sbjct: 371 IRHTKMNVGDIERLSVAVVVNYKTL---ADGKPLP--LTADQMKQIEDLTREAMGFSDKR 425

Query: 399 GDVINVTAVNF--LDPAGADMEPVSAPW--TDTLLRQSGSYANALAILAAVGLLIWFGLR 454
GD +NV F +D G ++ P+ + + Q + L +L +L +R
Sbjct: 426 GDTLNVVNSPFSAVDNTGGEL-----PFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVR 480

Query: 455 PLL--RDQNVKPAGTEVAIREAGEVATPNFIGGAESVGEGVQAVIGGPAAYADQMKTSLS 512
P L R + K A + +R+ E A + E L
Sbjct: 481 PQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDE----------------------QLQ 518

Query: 513 DLRQRMRMPAKL---RLEQMIEMDEERVAAVLKQWI 545
R R+ A++ R+ +M + D VA V++QW+
Sbjct: 519 QRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWM 554


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1052FLAGELLIN805e-19 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 79.7 bits (196), Expect = 5e-19
Identities = 45/227 (19%), Positives = 83/227 (36%), Gaps = 7/227 (3%)

Query: 4 ILTNSSALTALQTLASTNKSLESTQNRISTGLRISEASDNASYWSIATSMKSDNKANSAV 63
I TNS +L L + SL S R+S+GLRI+ A D+A+ +IA S+ K +
Sbjct: 4 INTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQA 63

Query: 64 QDALGLGAGKVDTAYSAINKIRESVDDIKTKLVSAMGA--STEDKGKIETEIKSIVANIN 121
G T A+N+I ++ ++ V A S D I+ EI+ + I+
Sbjct: 64 SRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEID 123

Query: 122 SALSNANYAGSNLLNGPTTDLNVVASYNRSGNAVTVDKITVKATDTDAKTMVKDIVDAGF 181
+ + G +L+ V + N I ++ D + + V+
Sbjct: 124 RVSNQTQFNGVKVLSQDNQMKIQVGA-----NDGETITIDLQKIDVKSLGLDGFNVNGPK 178

Query: 182 FTSASDDTAIGTALNTVETALASLATGAATLGAAKSQIDSQKSFLSA 228
+ D + + +T + + D+ +
Sbjct: 179 EATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPD 225


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1053PERTACTIN602e-10 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 59.7 bits (144), Expect = 2e-10
Identities = 88/384 (22%), Positives = 134/384 (34%), Gaps = 45/384 (11%)

Query: 2979 DTLLTNRLVIDGDTAGTTQVSVTNRGGLGAQTVEGIKIIQVGGASNGMFLLAGDYMFNGE 3038
D L+++LV+ D +G ++ V N G + + ++Q S F LA N +
Sbjct: 487 DLGLSDKLVVMRDASGQHRLWVRNSGS-EPASGNTMLLVQTPRGSAATFTLA-----NKD 540

Query: 3039 QAVVAGAYGYRLYKGGVSTPADGNWYLRSALL----NPETPTNPTDPETPLYQPGVPLYE 3094
V G Y YRL G +G W L A P P P P P
Sbjct: 541 GKVDIGTYRYRLAANG-----NGQWSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPP 595

Query: 3095 SYAGSLQQLNKLGTLQQRVGNRVWAKHPVPAQNDENGAGPS------------------- 3135
Q+ + Q G + A G +
Sbjct: 596 QPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRLN 655

Query: 3136 -GNNGIWARIEAAHAEFDPKQSTSRASYDADIWKFQTGIDGMFAETASGKFIGGVYVQYG 3194
G W R A + D + +D + F+ G D A +GG+ G
Sbjct: 656 PDAGGAWGRGFAQRQQLDNRAGRR---FDQKVAGFELGADHAVAVAGGRWHLGGLA---G 709

Query: 3195 TVSSSVSSPFGNGSIESSGYGAGATLTWYGESDFYIDGVAQINWFDSDLNSATLGRQLVD 3254
G+G + G T+ S FY+D + + ++D A V
Sbjct: 710 YTRGDRGFT-GDGGGHTDSVHVGGYATYIANSGFYLDATLRASRLENDFKVAGSDGYAVK 768

Query: 3255 GN-RAVGYSLSVETGQKIEIGEGWSLTPQAQLAYSAIRFDDFKDAFDTSVSPENDHDLTG 3313
G R G +S+E G++ +GW L PQA+LA + ++ A V E + G
Sbjct: 769 GKYRTHGVGVSLEAGRRFAHADGWFLEPQAELAVFRVGGGAYRAANGLRVRDEGGSSVLG 828

Query: 3314 RLGLAINRDAEWLDAQGRRVAMHI 3337
RLGL + + E A GR+V +I
Sbjct: 829 RLGLEVGKRIEL--AGGRQVQPYI 850


14BOV_A0007BOV_A0011N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0007-1161.657130Bmp family lipoprotein
BOV_A0009-1152.749334hypothetical protein
BOV_A00080152.569149putative enterobactin synthetase subunit D
BOV_A0010-1142.0000352,3-dihydroxybenzoate-2,3-dehydrogenase
BOV_A0011-1141.265261isochorismatase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0007LIPPROTEIN48452e-07 Mycoplasma P48 major surface lipoprotein signature.
		>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature.

Length = 428

Score = 45.4 bits (107), Expect = 2e-07
Identities = 60/268 (22%), Positives = 99/268 (36%), Gaps = 37/268 (13%)

Query: 36 IMLIVNGTLGDKSFFDSAANGMKMIKDKYGDEVETRILEIGDDPTKWEPVLLDASEQDWD 95
+++ G + DKSF SA +K I + G E+ +E + L A + W
Sbjct: 65 VLITDEGKIDDKSFNQSAFEALKAINKQTGIEINN--VEPSSNFESAYNSALSAGHKIW- 121

Query: 96 LIIAGTYQMAETVGSVAQQYPDK------KYILYDASVPYEEGGYDNVYSIQYKQNEGSY 149
++ G + + ++ K I D + E Y YS+Q+ E ++
Sbjct: 122 -VLNGFKHQQSIKQYI-DAHREELERNQIKIIGIDFDIETE---YKWFYSLQFNIKESAF 176

Query: 150 LGGLLAASLLKEGKLGDGAKNLGFLGGMDIPVINDFLVGYIAGA---QSVIPDAKI---- 202
G AS L E + + + GG P + F G+ G +KI
Sbjct: 177 TTGYAIASWLSEQD--ESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTS 234

Query: 203 AVSYAGSFMDAAKGKEL-------GLAQYRSGVSLGFVVASQTGLGQLSAAKETGKYVLG 255
V F K + A + + VA + A + G+YV+G
Sbjct: 235 PVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLANK-GQYVIG 293

Query: 256 VDSDQEAIFKDSDPAIAKQVVSSVLKNI 283
VDSDQ I ++++SVLK+I
Sbjct: 294 VDSDQGMIQD------KDRILTSVLKHI 315


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0008ENTSNTHTASED971e-26 Enterobactin synthetase component D signature.
		>ENTSNTHTASED#Enterobactin synthetase component D signature.

Length = 234

Score = 97.0 bits (241), Expect = 1e-26
Identities = 66/207 (31%), Positives = 102/207 (49%), Gaps = 12/207 (5%)

Query: 35 FVMARYSASKPLIDPSIPAPARAAPRRKAEFVAGQACAREALRLAGGPAVMPGRGDDHAP 94
F + + L P A +RKAE +AG+ A ALR G V PG GD P
Sbjct: 20 FDASSFREHDLLWLPHHDRLRSAGRKRKAEHLAGRIAAVHALREVGVRTV-PGMGDKRQP 78

Query: 95 IWPPGMVGSISHSKGCAIAVAARQSRYLGLGIDIERLQTESSARKIAPQILGESELRQLP 154
+WP G+ GSISH A+AV +RQ +GIDIE++ ++ +A ++AP I+ E + L
Sbjct: 79 LWPDGLFGSISHCATTALAVISRQR----IGIDIEKIMSQHTATELAPSIIDSDERQILQ 134

Query: 155 EAGRAFLVS--LAFSAKESLFKALYPAVQQFQSFHAAAFSFDGDRQQACLELAVDWSAQW 212
+ F ++ LAFSAKES++KA + F++A + + L +
Sbjct: 135 ASLLPFPLALTLAFSAKESVYKA-FSDRVTLPGFNSAKVTSLTATHISLHLLPAFAATMA 193

Query: 213 RRGQRFPIRFFCRDGFILTCTAIAAIS 239
R +F RD ++ T ++AI+
Sbjct: 194 ER--TVRTEWFQRDNSVI--TLVSAIT 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0010DHBDHDRGNASE2588e-89 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 258 bits (660), Expect = 8e-89
Identities = 114/254 (44%), Positives = 153/254 (60%), Gaps = 12/254 (4%)

Query: 15 GKRVIVTGAAQGIGRRVAERFLAEGATVIGLD-----------RQAGEADAPFRLIRLDI 63
GK +TGAAQGIG VA ++GA + +D EA D+
Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF-PADV 66

Query: 64 TDAAEVRAVSDGLKAEGKPLDILVNVAGVLKLGNSDRLSAEDWKTCMDVNASGPFHLLSQ 123
D+A + ++ ++ E P+DILVNVAGVL+ G LS E+W+ VN++G F+
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 124 WVPVFRDQRHGAIVNVASNAAHVPRLNMAAYCASKAALASFSHCVALELAPYGVRCNVVS 183
D+R G+IV V SN A VPR +MAAY +SKAA F+ C+ LELA Y +RCN+VS
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 184 PGSTRTAMLGAMLNDPSGEAQLVRGLPEQFKLGIPLGKIAMPDDIANVILFLASDQAGHV 243
PGST T M ++ D +G Q+++G E FK GIPL K+A P DIA+ +LFL S QAGH+
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 244 TMQDIVVDGGATLG 257
TM ++ VDGGATLG
Sbjct: 247 TMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0011ISCHRISMTASE336e-118 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 336 bits (863), Expect = e-118
Identities = 138/302 (45%), Positives = 188/302 (62%), Gaps = 18/302 (5%)

Query: 1 MAIPKIADYPMPQKAGFPANKTNWQPDPARAVLLIHDMQRYFLRFYDANGPLMGDLIGNL 60
MAIP I Y MP + P NK +W PDP RAVLLIHDMQ YF+ + A + +L N+
Sbjct: 1 MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI 60

Query: 61 VRLRNWAHENGVPVVYTAQPHEQPAGDRALLNDMWGPGLPAAAPEQQLIVDKLTPGATDT 120
+L+N + G+PVVYTAQP Q DRALL D WGPGL + P ++ I+ +L P D
Sbjct: 61 RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGL-NSGPYEEKIITELAPEDDDL 119

Query: 121 VLAKWRYSAFQRSDLLQRMKDWNRDQLIIGGIYAHIGCMVTAIDAFMNDIQPFMVGDAVA 180
VL KWRYSAF+R++LL+ M+ RDQLII GIYAHIGC+VTA +AFM DI+ F VGDAVA
Sbjct: 120 VLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVA 179

Query: 181 DFSEEEHRLALKYVATRCGAVIDTASLIRK----------------PATETTRDWLQKRV 224
DFS E+H++AL+Y A RC + T SL+ + T + ++K++
Sbjct: 180 DFSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQI 239

Query: 225 LELI-EDETELDPQENLILYGLDSLQVMKLSAELKERGVAVGFDDLARVPTLAAWWDLIE 283
EL+ E ++ QE+L+ GLDS+++M L + + G V F +LA PT+ W L+
Sbjct: 240 AELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLT 299

Query: 284 TK 285
T+
Sbjct: 300 TR 301


15BOV_A0053BOV_A0063N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0053520-0.871601putative lipoprotein
BOV_A0054419-1.112843P-type DNA transfer ATPase VirB11
BOV_A0055419-1.884229type IV secretion system protein VirB10
BOV_A0056320-2.372070P-type conjugative transfer protein VirB9
BOV_A0057421-2.617514type IV secretion system protein VirB8
BOV_A0058421-2.482511type IV secretion system protein VirB6
BOV_A0059219-1.743798P-type DNA transfer protein VirB5
BOV_A0060119-1.864943type IV secretion system protein VirB4
BOV_A0061014-1.207258type IV secretion system protein VirB3
BOV_A0062-1130.081389type IV secretion system protein VirB2
BOV_A0063-390.442374type IV secretion system protein VirB1
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0053OMPADOMAIN512e-10 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 51.5 bits (123), Expect = 2e-10
Identities = 32/144 (22%), Positives = 56/144 (38%), Gaps = 21/144 (14%)

Query: 47 FPQEPTAQATMWPARPPKQTVS--------VYFPQDVTVFRPTSAQ-INQLHTLLWPV-P 96
F Q A P + + V F + +P ++QL++ L + P
Sbjct: 191 FGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDP 250

Query: 97 KH--INVRGLTDNNCPPPGDTQVARVRALAIYNWLINQGVPASRI-TISYAPVKDYASN- 152
K + V G TD + ++ RA ++ ++LI++G+PA +I N
Sbjct: 251 KDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNT 310

Query: 153 -------APLSPGRVLNRRVDIEI 169
A L +RRV+IE+
Sbjct: 311 CDNVKQRAALIDCLAPDRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0055PF03544391e-05 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 39.2 bits (91), Expect = 1e-05
Identities = 26/122 (21%), Positives = 33/122 (27%), Gaps = 1/122 (0%)

Query: 33 VLLFLFVVGFIVVLLLLLVFHMRGN-AENNHHSDKTMVQTSTVPMRTFKLPPPPPPAPPE 91
LL + + G +V LL H TMV + + PPP P PE
Sbjct: 18 TLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPE 77

Query: 92 PPAPPPAPAMPIAEPAAAALSLPPLPDDTPAKDDVLDKSASALMVVTKSSGDTNAQTTNA 151
P P A P P P K K + +S N
Sbjct: 78 PEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARP 137

Query: 152 RI 153

Sbjct: 138 TS 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0056TYPE4SSCAGX512e-09 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 50.9 bits (121), Expect = 2e-09
Identities = 47/197 (23%), Positives = 72/197 (36%), Gaps = 58/197 (29%)

Query: 93 SHSNQSIDMAPEPGKWDTNLMVTTDQRMYDFDLRLMPGRNNQRVAYRVQFRYPAAAAAAA 152
S + SI+++P W TNL+V T++ +Y F LR+ N V+ YP ++
Sbjct: 262 SPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAYLTVKLEYPQRHEVSS 321

Query: 153 V----------AAAQKRVV-QARMNA---------------------------------- 167
V A Q+ ++ Q +N
Sbjct: 322 VIEEELKKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKEKIREEKQKIILDQAK 381

Query: 168 -----------RPSPVNWNYTMQVG--TNSASIAPTLAYDDGRFTYLRFPNNRDFPAAFL 214
+ +PV NY S I P+ +DDG FTY F N PA F+
Sbjct: 382 ALETQYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQPAIFV 441

Query: 215 VAEDKSESIVNSHIDPS 231
V D S+ ++ IDP+
Sbjct: 442 VQPDGKLSMTDAAIDPN 458


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0057PF043352156e-73 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 215 bits (550), Expect = 6e-73
Identities = 58/217 (26%), Positives = 104/217 (47%), Gaps = 7/217 (3%)

Query: 23 YDEALNWEAAHVRLIEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNAQT 82
++EA +WE + E+S++ AW +AG G + + +A + PLK PY++ V+ T
Sbjct: 14 FEEAASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNT 73

Query: 83 GAPDILTSLD-EKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSY 141
G I L + +++YD + KY+L+ YV RE + ++ ++ V ++S+ E +
Sbjct: 74 GEASIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRW 133

Query: 142 ASQFQGD--KALDKQYGSNVRTSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWI 199
+ ++ D ++ + V I + G + V F K + + + T +
Sbjct: 134 SRFYKTDNPQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNS---TKTDAV 190

Query: 200 ATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMG 236
ATI Y+ E R NPLG+ V SYR D E+
Sbjct: 191 ATIKYKVDGTP-SKEVDRFKNPLGYQVESYRADVEVP 226


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0058CHANLCOLICIN320.004 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 32.0 bits (72), Expect = 0.004
Identities = 16/31 (51%), Positives = 19/31 (61%), Gaps = 2/31 (6%)

Query: 305 SGSSGGGGSGSAKAGGESSYSAGGNAMWSPA 335
SGS GGGG G +K+ ESS + A WS A
Sbjct: 32 SGSGGGGGKGGSKS--ESSAAIHATAKWSTA 60


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0063PHAGEIV280.049 Gene IV protein signature.
		>PHAGEIV#Gene IV protein signature.

Length = 426

Score = 27.6 bits (61), Expect = 0.049
Identities = 7/57 (12%), Positives = 15/57 (26%)

Query: 134 TGGFKTKPGSPSYVQKVVASADVTTKPIAVVPMIRKTPDAAAAVAAPVKKRQPADRN 190
F PS +QK + + ++A + V + +
Sbjct: 78 ANNFDMVGSIPSIIQKYNPNNQDYIDELPSSDNQEYDDNSAPSGGFFVPQNDNVTQT 134


16BOV_A0107BOV_A0119N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0107-113-0.490545Bcr/CflA subfamily drug resistance transporter
BOV_A01082120.051747HlyD family secretion protein
BOV_A0109211-0.316033TetR family transcriptional regulator
BOV_A01102120.357649LuxR family transcriptional regulator
BOV_A01111151.274989hypothetical protein
BOV_A01121160.923466flagellar biosynthesis protein FlhB
BOV_A01130150.750041flagellar motor switch protein G
BOV_A0114-1110.870502putative flagellar motor switch protein FliN
BOV_A0115-1101.219098hypothetical protein
BOV_A011709-0.475232flagellar motor protein MotA
BOV_A0118011-0.859712hypothetical protein
BOV_A0119112-0.797379flagellar basal body rod protein FlgF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0107TCRTETB1036e-26 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 103 bits (259), Expect = 6e-26
Identities = 73/402 (18%), Positives = 148/402 (36%), Gaps = 19/402 (4%)

Query: 36 FMAILDIQIVSASLSEIQAGLGASTDEISWVQTSYLIAEVIMIPLSGFLGRLLSTRVLFT 95
F ++L+ +++ SL +I +WV T++++ I + G L L + L
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 96 ISAAGFTLASMLCATA-TNIEQMIVYRAIQGFIGGGMIPSVFAAAFTIF-PPSKRSIVSP 153
S++ + +I+ R IQG G P++ + P R
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQG-AGAAAFPALVMVVVARYIPKENRGKAFG 142

Query: 154 MIGLVATLAPTIGPTIGGYLSHAFSWHWLFLVNVGPGILVTIAAWNLIDFDEGDLSLLDK 213
+IG + + +GP IGG ++H W +L L+ P I + I L+ + ++ +
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLI---PMITI-ITVPFLMKLLKKEVRIKGH 198

Query: 214 FDWWGLFGMAAFLGSLEYVLEEGPRNDWLQDHTIFIMSIILTIGAIVFFYRVFTAEQPIV 273
FD G+ M+ + I+ + ++F + P V
Sbjct: 199 FDIKGIILMSVGIVFFMLFTTS---YSIS-------FLIVSVLSFLIFVKHIRKVTDPFV 248

Query: 274 DFRAFRNMNFAFGSLFSFVMGVGLYGLTYLYPLYLSMIRGYDALMIGEA-LFVSGLAMFF 332
D +N+ F G L ++ + G + P + + IG +F +++
Sbjct: 249 DPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVII 308

Query: 333 TAPVAGFLSNRMDPRLMMMIGFVGFAIGTWLVTGLTADWDFNELLMPQILRGCSLMLCMV 392
+ G L +R P ++ IG ++ +L + + + + L
Sbjct: 309 FGYIGGILVDRRGPLYVLNIGVTFLSVS-FLTASFLLETTSWFMTIIIVFVLGGLSFTKT 367

Query: 393 PINNLALGTLPPSLMKNASGLFNLTRNLGGAVGLAVINTILT 434
I+ + +L L N T L G+A++ +L+
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0108RTXTOXIND1102e-28 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 110 bits (276), Expect = 2e-28
Identities = 62/413 (15%), Positives = 127/413 (30%), Gaps = 89/413 (21%)

Query: 69 RVVLPSLVAVAAIGALWYGYNWWTVGRFMVSTDDAYVQGDIASIAPKVTGYIENIPVAAN 128
R+V ++ I + V + G I P ++ I V
Sbjct: 58 RLVAYFIMGFLVIAFILSV--LGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEG 115

Query: 129 QQVKAGDVIFQLDAGDYQIALDEANAKLATQ----------------------------- 159
+ V+ GDV+ +L A + + + L
Sbjct: 116 ESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPY 175

Query: 160 ----------------KETLIRIQAQTDAAKAALLQANADQQAAKAVLLNAQST------ 197
KE Q Q + L + A++ A + ++
Sbjct: 176 FQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKS 235

Query: 198 -MARVQKLHETRFV-------AQSELDNAQSALDQARARLAGADAQIASAKANISVLEAQ 249
+ L + + +++ A + L +++L +++I SAK ++
Sbjct: 236 RLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQL 295

Query: 250 Y--------NEAASSTKSLELARDKAARDLSFTSLRALFDGVIGNLS-GKKGDLVAPGQK 300
+ + + L L K + +RA + L +G +V +
Sbjct: 296 FKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAET 355

Query: 301 IAALVPVDQ-LYIDANFKETQLAHIKVGETAHIYVDAIDGTKF---DGKVASIAPASGAV 356
+ +VP D L + A + + I VG+ A I V+A T++ GKV +I +
Sbjct: 356 LMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA--- 412

Query: 357 FSLLPPENATGNFTKVVQRVPVRITIPKEAFESGK---IRAGLSVIVDVDTRT 406
+ G V+ + K + +G++V ++ T
Sbjct: 413 ----IEDQRLGLVFNVIISIEE-----NCLSTGNKNIPLSSGMAVTAEIKTGM 456


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0109HTHTETR767e-19 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 75.8 bits (186), Expect = 7e-19
Identities = 34/155 (21%), Positives = 65/155 (41%), Gaps = 9/155 (5%)

Query: 54 RSQILEGAQSVFLRMGFDAASMNDITREAGVSKGTIYVYFNSKEDLFVALCEHYRQTLFS 113
R IL+ A +F + G + S+ +I + AGV++G IY +F K DLF + E +
Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72

Query: 114 SFIGQLEKGFSNRQELIDFGVALTTLITSSIAIRAQRIVVGVSERKPELA------ARFY 167
+ K + + L ++ S++ +R+++ + K E +
Sbjct: 73 LELEYQAKFPGDPLSV--LREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQ 130

Query: 168 ERGPKRSHAIMAQSLQAMIDAGVL-EQHDVTRTAY 201
S+ + Q+L+ I+A +L R A
Sbjct: 131 RNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAI 165


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0112TYPE3IMSPROT2888e-98 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 288 bits (738), Expect = 8e-98
Identities = 95/344 (27%), Positives = 164/344 (47%), Gaps = 9/344 (2%)

Query: 10 KTEEATEQKIRDALDKGNMPFSREAPILAGIASFLVIAVFVAAPAATRLA---VFLRELI 66
KTE+ T +KIRDA KG + S+E A I + + + ++ + + E
Sbjct: 5 KTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAEQS 64

Query: 67 DRPEDWLLNSAEDATRLFSVLALAVGAALVPVFIIIPLAGIAASAFQNAPRFVGERIRPQ 126
P L + + + L P+ + L IA+ Q GE I+P
Sbjct: 65 YLPFSQAL------SYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPD 118

Query: 127 ASRISPLKGWQRIFGRAGQVEFLKSLAKLLAASVIVFLVFFKGNSLFTDAVATDPGALPE 186
+I+P++G +RIF VEFLKS+ K++ S++++++ +
Sbjct: 119 IKKINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITP 178

Query: 187 FLRKNVVRLLVANVLAIAAIAGFDLAWSRIHWRQELRMTRQEVKDELKQSEGDPLVKSRL 246
L + + +L+V + I+ D A+ + +EL+M++ E+K E K+ EG P +KS+
Sbjct: 179 LLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKR 238

Query: 247 RSLGRDRARRRMINAVPTATLIVANPTHFSVALRYKPNEDAAPVVVAKGQDLIALKIREI 306
R ++ R M V ++++VANPTH ++ + YK E P+V K D +R+I
Sbjct: 239 RQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKI 298

Query: 307 AASHSIPVFEDVQLARALYKQVNVDQMIAPEFYKAVAELIRIIN 350
A +P+ + + LARALY VD I E +A AE++R +
Sbjct: 299 AEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLE 342


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0113FLGMOTORFLIG1906e-60 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 190 bits (484), Expect = 6e-60
Identities = 80/330 (24%), Positives = 158/330 (47%), Gaps = 13/330 (3%)

Query: 22 VDTLTGPQKAAAILVAIGRPAAARLLKHFNAEDLRKLAGHARTLEPISPLDFEQLVKQFE 81
V LTG QKAA +LV+IG ++++ K+ + E++ L LE I+ + ++ +F+
Sbjct: 12 VSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEFK 71

Query: 82 DLF-AEGAPVSEAAQRFEGLLRDTLPQEEVDAVLEDRVQPQLVQESVWIQVARLPMESLQ 140
+L A+ LL +L ++ ++ + +Q + V R ++
Sbjct: 72 ELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSA--LQSRPFEFVRRADPANIL 129

Query: 141 AYLGNQHPQIIAYIVSRLPSDLAARLFVALPPQLRNAVVQRSLHIGNVAPAAAELLEDVL 200
++ +HPQ IA I+S L A+ + +LP +++ V +R + +P +E VL
Sbjct: 130 NFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVERVL 189

Query: 201 RRDL-----LGRSDAGPLKAHHGQIANIFNQLDRSEMEELMASLQDLNRDDLNRIKAKLF 255
+ L + AG + + I N DR + ++ SL++ + + IK K+F
Sbjct: 190 EKKLASLSSEDYTSAGGVDN----VVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMF 245

Query: 256 TFEDIERLSQRARLLLFDEVQTEQIATALREADPKLQELVLSSLSARGRRMVESELSAEQ 315
FEDI L R+ + E+ +++A AL+ D +QE + ++S R M++ ++
Sbjct: 246 VFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEF-L 304

Query: 316 GNITSQNIAEARRIIARIAIDLSERGIITL 345
G +++ E+++ I + L E+G I +
Sbjct: 305 GPTRRKDVEESQQKIVSLIRKLEEQGEIVI 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0114FLGMOTORFLIN885e-26 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 88.4 bits (219), Expect = 5e-26
Identities = 37/84 (44%), Positives = 59/84 (70%), Gaps = 3/84 (3%)

Query: 32 SASKPNLDLIMGIPVDVQVVLGGTTMPVSSLMKLGRGAVITLDKQIGDPVDIVVNGRVIA 91
S + ++DLIM IPV + V LG T M + L++L +G+V+ LD G+P+DI++NG +IA
Sbjct: 48 SGAMQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIA 107

Query: 92 RGEVIVLEDDSSRFGVSLTEIIGK 115
+GEV+V+ D ++GV +T+II
Sbjct: 108 QGEVVVVAD---KYGVRITDIITP 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0115IGASERPTASE280.015 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 28.1 bits (62), Expect = 0.015
Identities = 12/66 (18%), Positives = 29/66 (43%), Gaps = 1/66 (1%)

Query: 68 AKLDARLAETQRVRREMDEAIEELNRLRKALSRDMRQARILAETPPPRQEKTPSAAEQPL 127
A+ + ETQ + +E+ + + + ++ ++ P+QE++ + Q
Sbjct: 1086 AQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQV-SPKQEQSETVQPQAE 1144

Query: 128 PADKNA 133
PA +N
Sbjct: 1145 PAREND 1150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0119FLGHOOKAP1310.004 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 31.1 bits (70), Expect = 0.004
Identities = 5/33 (15%), Positives = 18/33 (54%)

Query: 3 NNSIYVGLSSLITLERRMDAIAHNVANASTVGF 35
++ I +S L + ++ ++N+++ + G+
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGY 33


17BOV_A0136BOV_A0144N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A01360160.949129flagellar basal body rod protein FlgC
BOV_A0137-1171.429605flagellar hook-basal body protein FliE
BOV_A0138-1150.968536flagellar basal body rod protein FlgG
BOV_A0139-1150.235161flagellar basal body P-ring biosynthesis protein
BOV_A0141113-0.154245hypothetical protein
BOV_A0142-114-0.236053flagellar basal body L-ring protein
BOV_A0143016-0.335799putative flagellar protein FliL
BOV_A0144017-0.348345flagellar biosynthesis protein FliP
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0136FLGHOOKAP1310.001 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 31.1 bits (70), Expect = 0.001
Identities = 10/38 (26%), Positives = 20/38 (52%)

Query: 99 NVNMVVEMADMREANRSYEANLQVVRQARELISMTIDL 136
VN+ E +++ + Y AN QV++ A + I++
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0137FLGHOOKFLIE359e-06 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 35.4 bits (81), Expect = 9e-06
Identities = 22/81 (27%), Positives = 36/81 (44%), Gaps = 2/81 (2%)

Query: 33 QAVPAAPGASFGEVLSQMTGSVSQKLQAAEATSIQGIKG--DAPVRDVVSSVMEAEQSLQ 90
Q P SF L +S AA + + G + DV++ + +A S+Q
Sbjct: 23 QESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQ 82

Query: 91 TVIAIRDKIVQAYLEISRMPI 111
I +R+K+V AY E+ M +
Sbjct: 83 MGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0138FLGHOOKAP1439e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 42.6 bits (100), Expect = 9e-07
Identities = 10/45 (22%), Positives = 21/45 (46%)

Query: 213 SIKQGYLEASNVDPVKEITDLITAQRAYEMNSKVIQAADEMAATV 257
+ S V+ +E +L Q+ Y N++V+Q A+ + +
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDAL 542



Score = 39.2 bits (91), Expect = 1e-05
Identities = 21/85 (24%), Positives = 33/85 (38%), Gaps = 15/85 (17%)

Query: 4 LTIAATGMNAQQLNLEVIANNIANINTTGFKRARAEFTDLLYQSERTAGVPNQANQAIVP 63
+ A +G+NA Q L +NNI++ N G+ R + T +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTR------------QTTIMAQANSTLG--- 48

Query: 64 EGALVGLGVQTAAVRNLHIQGSFNQ 88
G VG GV + V+ + NQ
Sbjct: 49 AGGWVGNGVYVSGVQREYDAFITNQ 73


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0142FLGLRINGFLGH1884e-62 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 188 bits (479), Expect = 4e-62
Identities = 67/248 (27%), Positives = 102/248 (41%), Gaps = 27/248 (10%)

Query: 2 KNSMNKAILAVAMVLLLAGCA---TKPEEIG-----RAPDLSPVAAHLGMQNNPQFNGYP 53
KN+ + ++ +VL L GCA + P G P +PVA Q+ Q Y
Sbjct: 3 KNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQS-AQPINYG 61

Query: 54 ARPGKASYSLWDQRSTNFFKDPRAATPGDVLTVIISINDRANLDNKTDRERVSKGIYGGG 113
+P F+D R GD LT+++ N A+ + + R K G
Sbjct: 62 YQP--------------LFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT--NFG 105

Query: 114 GSFAASSITGAAAGGDMDASVNTHSDSKSKGKGTIERSEDIRLQIAAIVTDTLPNGNLII 173
+ G A V + GKG S + V L NGNL +
Sbjct: 106 FDTVPRYLQGLFGNAR--ADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHV 163

Query: 174 RGSQEVRVNNELRVLNVAGVVRPRDISGNNTISYDKIAEARISYGGRGRLSEIQQPPYGQ 233
G +++ +N + +GVV PR ISG+NT+ ++A+ARI Y G G ++E Q + Q
Sbjct: 164 VGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQ 223

Query: 234 QILDQFSP 241
+ SP
Sbjct: 224 RFFLNLSP 231


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0144FLGBIOSNFLIP2481e-85 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 248 bits (636), Expect = 1e-85
Identities = 100/234 (42%), Positives = 149/234 (63%), Gaps = 3/234 (1%)

Query: 13 LALTVSAQAQQALPLDKLLPAGSGAASGQIVQLFGLLTVLSIAPGLLIMVTSFTRFAIAF 72
L L Q + G G + VQ +T L+ P +L+M+TSFTR I F
Sbjct: 12 LWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVF 71

Query: 73 SLLRSGLGLQTAPASMVMISLALFMTFYVMAPAFDRAWNNGVQPLMRNEITQEAAFGEIS 132
LLR+ LG +AP + V++ LALF+TF++M+P D+ + + QP +I+ + A + +
Sbjct: 72 GLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGA 131

Query: 133 QPFREFMMAQVRDKDLRLFEDLAD-PSFRTSDDGIVDFRVLVPAFMISELRRGFEIGFLI 191
QP REFM+ Q R+ DL LF LA+ + + V R+L+PA++ SEL+ F+IGF I
Sbjct: 132 QPLREFMLRQTREADLGLFARLANTGPLQGPEA--VPMRILLPAYVTSELKTAFQIGFTI 189

Query: 192 VLPFLVIDLVVATLTMSMGMMMLPPTVISLPFKILFFVLIDGWNILVGSLIRSF 245
+PFL+IDLV+A++ M++GMMM+PP I+LPFK++ FVL+DGW +LVGSL +SF
Sbjct: 190 FIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243


18BOV_A0359BOV_A0366N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0359-1120.188975exopolysaccharide biosynthesis protein Bme12
BOV_A03600130.086414exopolysaccharide biosynthesis protein Bme11
BOV_A0361-1110.208560fucose synthetase family protein
BOV_A0362-110-0.080238GDP-mannose 4,6-dehydratase
BOV_A0363-1120.426622putative glycosyltransferase
BOV_A0364-1110.448312glycosyl transferase family protein
BOV_A0365-110-0.045748glycosyl transferase family protein
BOV_A0366-1130.023555outer membrane protein Omp31
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0359CHANLCOLICIN366e-04 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 35.8 bits (82), Expect = 6e-04
Identities = 36/162 (22%), Positives = 61/162 (37%), Gaps = 12/162 (7%)

Query: 234 SGATCDGAEYGDCRRNRRSIHRAGKAANRQALKNNNEAPAMTEILASPAIQNLRNEEARV 293
SG+ G + G + +IH K + Q K E A + A + N +A
Sbjct: 32 SGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALT 91

Query: 294 QRHLD----ELKANGALKSAEIPVLMAERESLKQQITA--------QVDEIIKSLSNEIR 341
QR D L+ N + + + A +++ + + + ++ +
Sbjct: 92 QRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQ 151

Query: 342 IAVQRRTSLEKELKEAETDLAKANQAQVRAAQLDREANASRV 383
A QRR +E+E E E L A + R A L EA A +
Sbjct: 152 EAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEI 193


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0360RTXTOXIND742e-16 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 74.1 bits (182), Expect = 2e-16
Identities = 25/213 (11%), Positives = 73/213 (34%), Gaps = 7/213 (3%)

Query: 151 VTAGMQLI-----SAQQEYADLQMQITAQTIRRARFEAELKGTDFTYAVPEQTAANKDMV 205
V G L+ A+ + Q + + + R++ + + +
Sbjct: 118 VRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQ 177

Query: 206 ALTRHMVEGERTLFDVRRNNLAAERRALEAQAASYGDEITTLQQSIKLHDTEIQLLQENV 265
++ V +L + + ++ E E T+ I ++ ++ + +
Sbjct: 178 NVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRL 237

Query: 266 NSSKSLVDRGLAAKSSLRDMERDLSSTRRDALELASFLARARQNQLAVEQRIANLDETRR 325
+ SL+ + AK ++ + E + S L + L+ ++ + + +
Sbjct: 238 DDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFK 297

Query: 326 SEAASSLQEIDLNMA--RMQRRSNSQLEAMAEI 356
+E L++ N+ ++ N + + + I
Sbjct: 298 NEILDKLRQTTDNIGLLTLELAKNEERQQASVI 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0361NUCEPIMERASE805e-19 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 79.8 bits (197), Expect = 5e-19
Identities = 71/352 (20%), Positives = 130/352 (36%), Gaps = 72/352 (20%)

Query: 19 KIFVAGHTGMVGSAILRRLQHED-----CDIITAAHSVL-------------------DL 54
K V G G +G + +RL D + + V DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 T-RQGPTENFISGHRPDVIIIAAARVGGILANSRFPADFLYDNLAIGMNLIHAAHQIGVE 113
R+G T+ F SGH + + I+ R + + P + NL +N++ ++
Sbjct: 62 ADREGMTDLFASGH-FERVFISPHR-LAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 114 RLLWLGSSCIYPRDAAQPLTEDALLTGPLEPTNEAYAIAKIAGLKYAQSCARQF-----G 168
LL+ SS +Y + P + D + P+ YA K A A + + + G
Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSL----YAATKKANELMAHTYSHLYGLPATG 175

Query: 169 DRFITAMPTNLYGPNDNFDPTSSHVLPALIRRVHEARMRGAEEVVLWGSGKPLREFLHVD 228
RF T +YGP D L + + E + + ++ GK R+F ++D
Sbjct: 176 LRFFT-----VYGPWGRPD----MALFKFTKAMLE-----GKSIDVYNYGKMKRDFTYID 221

Query: 229 DLADACLHLL------------------RFYNGIEPVNIGSGEEISIKELALTVARIVGY 270
D+A+A + L NIG+ + + + + +G
Sbjct: 222 DIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGI 281

Query: 271 EGRFEHDLSKPDGTPRKLLDTSRI-EALGWQPRIHLEDGLR---DVYRNWLE 318
E + +P DT + E +G+ P ++DG++ + YR++ +
Sbjct: 282 EAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDFYK 333


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0362NUCEPIMERASE865e-21 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 86.0 bits (213), Expect = 5e-21
Identities = 68/354 (19%), Positives = 126/354 (35%), Gaps = 50/354 (14%)

Query: 7 LFVGVTGQDGAYLSELLLGKGYRVHGLKRRSSSFNMARIDHLYQDPHEEDIRFRLHFGDL 66
L G G G ++S+ LL G++V G+ + ++++ + + +F H DL
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQF--HKIDL 61

Query: 67 TDATNLCRVIQEVQPDEIYNLGAQSHVQVSFETPEYTANADALGTLRLLESMRILGLGKS 126
D + + + ++ + V+ S E P A+++ G L +LE R +
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ-- 119

Query: 127 CRFYQASTSELFGNSSHHAQNEQTPFA-------PRSPYATAKLYAYWTTVNYRDAYGFH 179
AS+S ++G N + PF+ P S YA K Y YG
Sbjct: 120 -HLLYASSSSVYGL------NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLP 172

Query: 180 ASNGILFNHESPLRGETFVTRKITRAVA---AIERGLQDRLRLGNLEARRDWGHARDYVE 236
A+ F P K T+A+ +I+ + +RD+ + D E
Sbjct: 173 ATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSID-------VYNYGKMKRDFTYIDDIAE 225

Query: 237 GMWRILQEDTPDDYVLATGETHTVREFIEHAFKAVDKQIVWHGDG--------VDEVGID 288
+ R+ V+ +T E A ++ G+ + ++
Sbjct: 226 AIIRLQD-------VIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQA--LE 276

Query: 289 RKTGNCLIEIDPRY--FRPNEVDFLMGDASKAKDRLGWQHTTGFENLVAEMVDW 340
G IE +P +V D + +G+ T ++ V V+W
Sbjct: 277 DALG---IEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNW 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0366OMPADOMAIN503e-09 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 49.5 bits (118), Expect = 3e-09
Identities = 52/250 (20%), Positives = 87/250 (34%), Gaps = 69/250 (27%)

Query: 1 MK--SVILASIAAMFATSAMAADVVVSEPSAPTAAPVDTFSWTGGYIGINAGYAGGKFKH 58
MK ++ +A A FAT A AAP D +TG +G + Y F +
Sbjct: 1 MKKTAIAIAVALAGFATVA-------------QAAPKDNTWYTGAKLG-WSQYHDTGFIN 46

Query: 59 PFSSFDKEDGEHVSGSPDVTAGGFVG-------GVQAGYNWQLDNGVVLGAETDFQGLSV 111
+ + AG F G G + GY+W +
Sbjct: 47 NNGPTHENQ---------LGAGAFGGYQVNPYVGFEMGYDW-------------LGRMPY 84

Query: 112 TGSISAGASSLEGNAETKVEWFGTVRARLGYTATERLMVYGTGGLAYGKVKSAFNLGDDA 171
GS+ GA +G + A+LGY T+ L +Y G + + N+
Sbjct: 85 KGSVENGAYKAQG---------VQLTAKLGYPITDDLDIYTRLGGMVWRADTKSNVYGK- 134

Query: 172 PALHTWSDKTKAGWTLGAGAEYAINNNWTLKSEYLYT-DLGKRNLVDVDNSFLESKVNFH 230
+ T G EYAI + EY +T ++G + + + +
Sbjct: 135 ------NHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGT-------RPDNG 181

Query: 231 TVRVGLNYKF 240
+ +G++Y+F
Sbjct: 182 MLSLGVSYRF 191


19BOV_A0401BOV_A0413N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0401-1130.909210TetR family transcriptional regulator
BOV_A0402-2111.122690HlyD family secretion protein
BOV_A0403-1110.140392ABC transporter ATP-binding protein
BOV_A0404-1110.042745ABC transporter permease
BOV_A0405-110-0.011532hypothetical protein
BOV_A0406-111-0.318854putative taurine ABC transporter permease
BOV_A0407-111-0.636662ABC transporter ATP-binding protein
BOV_A0408014-1.169422ABC transporter substrate-binding protein
BOV_A0409115-1.304355MarR family transcriptional regulator
BOV_A0410215-1.362327putative transporter
BOV_A0411014-0.210107HlyD family secretion protein
BOV_A04132170.376422putative transcriptional regulatory protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0401HTHTETR722e-17 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 71.6 bits (175), Expect = 2e-17
Identities = 29/179 (16%), Positives = 60/179 (33%), Gaps = 13/179 (7%)

Query: 9 PPQASLSSEQTRKALIVAALRLFGAKGYEATSTREIASLAKANIGSIAYHFGGKEGLRLA 68
+ +++TR+ ++ ALRLF +G +TS EIA A G+I +HF K L
Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 69 AADYIVETFRLIAAQALGNFQPAGFSREEREDARAQMISALERMVHFIVARPETGEIVQF 128
+ + + F D + + L ++ V +++
Sbjct: 62 IWELSESNIGELELEYQAKF---------PGDPLSVLREILIHVLESTVTEERRRLLMEI 112

Query: 129 LLRELAHPTAA--LDRIYKGVFEPVHKRLCMIWEAA--TGEAAESEATRLVVFTLVGQI 183
+ + + + + + + R+ + TR + G I
Sbjct: 113 IFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYI 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0402RTXTOXIND599e-12 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 58.7 bits (142), Expect = 9e-12
Identities = 21/131 (16%), Positives = 47/131 (35%), Gaps = 5/131 (3%)

Query: 27 VEGEFVQLAPLQVAQVRDIAVKRGDRVEAGEPIATVEDTDARIAVAQAEAALAQAQAQLA 86
G ++ P++ + V++I VK G+ V G+ + + A + +++L QA+ +
Sbjct: 92 HSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQT 151

Query: 87 NLQVGKRPEEIAVLEAAVRSARAQADDAQRTLLRTRDLARRGVATQAQLDDAATQLEVAE 146
Q+ +E D+ + ++ R + Q Q E
Sbjct: 152 RYQI-----LSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKE 206

Query: 147 AAIGQSTANLA 157
+ + A
Sbjct: 207 LNLDKKRAERL 217



Score = 44.8 bits (106), Expect = 3e-07
Identities = 27/257 (10%), Positives = 79/257 (30%), Gaps = 25/257 (9%)

Query: 67 ARIAVAQAEAALAQAQAQLANLQVGKRPEEIAVLEAAVR---SARAQADDAQRTLLR-TR 122
+ +AE A+ + + + A+ + + +
Sbjct: 207 LNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVN 266

Query: 123 DLARRGVATQAQLDDAATQLEVAEAAIGQSTANLAVGKLPARPEEIKAAENAVKSAEAQL 182
+L ++QL+ +++ A+ T L ++++ + + +L
Sbjct: 267 ELR----VYKSQLEQIESEILSAKEEYQLVTQLFKNEIL----DKLRQTTDNIGLLTLEL 318

Query: 183 QTAKWQRAQRTIEAPAAGRITDV-VRNPGDIAGPSAPVLTMLPDGAVKL-KVYIPEERFS 240
+ ++ I AP + ++ + V G + + ++ ++P+ + +
Sbjct: 319 AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIG 378

Query: 241 DVAVGSVLSVHCDG----CAPGLQARVSYVSPD---PEFTPPVIYSLETRQKLVYLVEAH 293
+ VG + + L +V ++ D + V + +
Sbjct: 379 FINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVI--ISIEENCLSTG 436

Query: 294 PVDPASPLQPGQIVDVD 310
+ PL G V +
Sbjct: 437 --NKNIPLSSGMAVTAE 451


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0404ABC2TRNSPORT499e-09 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 48.8 bits (116), Expect = 9e-09
Identities = 46/173 (26%), Positives = 76/173 (43%), Gaps = 8/173 (4%)

Query: 206 ALTRETERGTMENLLAMPATPAEIMLGKIL---PYLAVGGVQMVLVAAKLIFGVPFVGSL 262
A R + T E +L +I+LG++ A+ G + +VAA L G SL
Sbjct: 90 AFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAAL--GYTQWLSL 147

Query: 263 TLLLSSVLIFVLSLALLGYTISTVSRTQMQAMQLTFFFFLPSLMLSGFMFPFRGMPDWAQ 322
L + + L+ A LG ++ ++ + + P L LSG +FP +P Q
Sbjct: 148 LYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQ 207

Query: 323 ALGNIFPLTYFLRIVRAVMLKGAGLADIAGEVMALILF--VFLFAGLALLRFR 373
PL++ + ++R +ML D+ V AL ++ + F ALLR R
Sbjct: 208 TAARFLPLSHSIDLIRPIMLGHPV-VDVCQHVGALCIYIVIPFFLSTALLRRR 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0407PF05272280.035 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.035
Identities = 11/20 (55%), Positives = 13/20 (65%)

Query: 33 LIGPSGCGKSTLLRFLGGLE 52
L G G GKSTL+ L GL+
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0410TCRTETB417e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 41.4 bits (97), Expect = 7e-06
Identities = 37/200 (18%), Positives = 71/200 (35%), Gaps = 12/200 (6%)

Query: 57 IQGSLGATLTETTWLVAAYMAPFASLTILLLKIRTQFGLRRFAEVAIAVFLVASLLHLLV 116
I T W+ A+M F+ T + K+ Q G++R I + S++ +
Sbjct: 40 IANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVG 99

Query: 117 YDVWS-AIPVRFI--AGAAAVPISSVGVLYMLEAFPPDKKRTWGLSLALTCSGAAGPFAR 173
+ +S I RFI AGAAA P + V + + P + R L +
Sbjct: 100 HSFFSLLIMARFIQGAGAAAFPALVMVV---VARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 174 IISPMLFDIGQWQQLYMLEIGLALACFAVVYVLPLTQIPRAKVLHWLDFVAYTFIAIGFG 233
I M+ W L ++ + + ++ +L ++ D +++G
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLK----KEVRIKGHFDIKGIILMSVGI- 211

Query: 234 ALVVVLVQGKNYWWFEAPWI 253
+ +L F +
Sbjct: 212 -VFFMLFTTSYSISFLIVSV 230


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0411RTXTOXIND1048e-27 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 104 bits (260), Expect = 8e-27
Identities = 63/414 (15%), Positives = 130/414 (31%), Gaps = 85/414 (20%)

Query: 3 VSKKYIRGGFAILIGLLGVFIILWAWQLPPFKHSVETTDNAYVRGQVTVMSPQVSGYITK 62
VS++ + I+ L+ F + + L + G+ + P + + +
Sbjct: 53 VSRRPRLVAYFIMGFLVIAF--ILSV-LGQVEIVATANGKLTHSGRSKEIKPIENSIVKE 109

Query: 63 VNVTDYEQVKKGDLLFEID------------SRSYQQKLDQA------------------ 92
+ V + E V+KGD+L ++ S Q +L+Q
Sbjct: 110 IIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELK 169

Query: 93 ----------------------LAALDSKKAALANSEQSQRSAEATIKAREAQISGAKAA 130
+ + E + A A+I+ +
Sbjct: 170 LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENL 229

Query: 131 LEVAKANTVRVDTLLPRG-------VTTQSSADTAHGNMLQAQAAVDQAEAALAVAKEDL 183
V K+ +LL + + ++ A + ++ ++Q E+ + AKE+
Sbjct: 230 SRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEY 289

Query: 184 --------RTIIVAREGLNADLHNAEAAVALARIDLQNTKILAPRDGKLGEVGVR-LGQY 234
I+ ++ +A Q + I AP K+ ++ V G
Sbjct: 290 QLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGV 349

Query: 235 VTAGTQLVSLVP--AVKWIMANFKETQLYGMKVGQPVTLTVDAL---RHAELKGHIEAFS 289
VT L+ +VP + A + + + VGQ + V+A R+ L G ++ +
Sbjct: 350 VTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNIN 409

Query: 290 PATGSEFSVIKSDNATGNFTKITQRLSVRISIDEGQPDAELLAPGMSVVVRVDT 343
D G + + + L+ GM+V + T
Sbjct: 410 LDA-------IEDQRLGLVFNVIISIEENCLSTGNKN--IPLSSGMAVTAEIKT 454


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0413HTHFIS951e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 94.9 bits (236), Expect = 1e-24
Identities = 40/138 (28%), Positives = 66/138 (47%), Gaps = 3/138 (2%)

Query: 4 GAHILIVDDDKDIRDLLHEFLKRRGMHVSIACNGDEMLDVLSRTPIDLVILDVMLPGKSG 63
GA IL+ DDD IR +L++ L R G V I N + ++ DLV+ DV++P ++
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 64 IEICQDVR-RTSRVPIIMLTAIADAADKILGLEIGADDYIAKPFDPRELLARIRAVLRRF 122
++ ++ +P+++++A I E GA DY+ KPFD EL+ I L
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 123 EGNRSPQRALTQIYRFAG 140
+ R P +
Sbjct: 123 K--RRPSKLEDDSQDGMP 138


20BOV_A0857BOV_A0865N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A0857-110-0.041425AcrB/AcrD/AcrF multidrug efflux protein
BOV_A0859-2110.144417HlyD family secretion protein
BOV_A08600150.611418hypothetical protein
BOV_A08620140.622771L-lactate dehydrogenase
BOV_A08630151.070198outer surface protein
BOV_A08640141.568088putative alanine catabolic operon
BOV_A0865-2101.518320alanine racemase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0857ACRIFLAVINRP460e-147 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 460 bits (1186), Expect = e-147
Identities = 229/1043 (21%), Positives = 432/1043 (41%), Gaps = 61/1043 (5%)

Query: 7 LSDWALNHRSLVWYFMLVFLVAGLVSYLDLGREEDPEFTVKTMVVQANWPGASIDETLNQ 66
++++ + W ++ ++AG ++ L L + P + V AN+PGA +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 67 VTDRLEKKLEELDTLDYTRSVTT-AGKSVVFVFLKDTTRAQDVKKSWTEVRHLLTDIQNT 125
VT +E+ + +D L Y S + AG + + + T D + +V++ L
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGT---DPDIAQVQVQNKLQLATPL 117

Query: 126 LPQGVYGPY-FNDQFGDVFGNIYAFTADGLSMRQ--LRDYAED-VRTKILTIPNAGKVEL 181
LPQ V ++ + + F +D Q + DY V+ + + G V+L
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 182 IGAQDEAIYLEFSTRQVAALGLDQQAILKALQDQNAITPSGVVQAGPE------RISVRV 235
GAQ A+ + + L ++ L+ QN +G + P S+
Sbjct: 178 FGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 236 SGQFNSEADLRAVNLRVNN--RFFRLSDVATITRGYVDPPTSIFRVNGEDAIGLGIGMKP 293
+F + + V LRVN+ RL DVA + G + I R+NG+ A GLGI +
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELG-GENYNVIARINGKPAAGLGIKLAT 295

Query: 294 NGNLLEFGAELDKMMDQVTAELPVGVKVFKVADQPQVVKEAVSGFTQALFEAVVIVLAVS 353
N L+ + + ++ P G+KV D V+ ++ + LFEA+++V V
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVM 355

Query: 354 FVSLG-LRAGFVVSLSIPLVLAITFLSMSLMDISLQRVSLGALIIALGLLVDDAMIAVEM 412
++ L +RA + ++++P+VL TF ++ S+ +++ +++A+GLLVDDA++ VE
Sbjct: 356 YLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVEN 415

Query: 413 MV-ARLEHGDPINKAATYVYSHTAFPMLTGTLVTIAGFIPIGLNNSQAGEYTFTLFVVIA 471
+ +E P +A S ++ +V A FIP+ G + I
Sbjct: 416 VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIV 475

Query: 472 VSLLVSWVVAVLFAPLLGVTFLPKKMKPHEEKRSRFFEAFSRVLLLSM-----------R 520
++ +S +VA++ P L T L H E + FF F+ S+
Sbjct: 476 SAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILG 535

Query: 521 HKWTTIITTIVLFVISVFGMGFIERQFFPQSDRPELVLDWTLPQNSSIADTKAQMERFEE 580
++ ++ V + F P+ D+ + LP ++ T+ +++ +
Sbjct: 536 STGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTD 595

Query: 581 TMLKDN-PDVEHWSTYVGQGAIRFVLSFDVQPANPYFGQMVAVARDVEARDRLKAKFEAA 639
LK+ +VE T G SF Q N + + D A+
Sbjct: 596 YYLKNEKANVESVFTVNG-------FSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIH 648

Query: 640 -FRKDYVGTDVYVKYLELGPPV---GRPVQYRI-----SGPDVQKLRGIAQDFAGILS-A 689
+ + P + G + +G L G+ +
Sbjct: 649 RAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQH 708

Query: 690 DKRLGVVNYNWNEPGRVIRVDVMQDKARKLGISSKDIATTLNGVVGGITITQVRDSIYLI 749
L V N E +++V Q+KA+ LG+S DI T++ +GG + D +
Sbjct: 709 PASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVK 768

Query: 750 DVIARAQAHERDSIDTLQSLQVATGNGTSVPLAAIANFRYELEQPVIYRRSRIPTITVAA 809
+ +A A R + + L V + NG VP +A + P + R + +P++ +
Sbjct: 769 KLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQG 828

Query: 810 GIIDKTMPDTIARDLGPAIKTFADRLPAGYYIQTAGTVEESGKSQGPIAAVVPLMLFVMA 869
P T + D ++ A +LPAG G + S A+V + V+
Sbjct: 829 EA----APGTSSGDAMALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVF 884

Query: 870 TVLMVQLQSFQRLFLVVAVAPLGLIGVVAALLPSGKPLGFVAILGVLALIGILIRNSVIL 929
L +S+ V+ V PLG++GV+ A + ++G+L IG+ +N++++
Sbjct: 885 LCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILI 944

Query: 930 IVQVEE-DIQEGLHPWDAVMKASQHRMRPIALTAAAASLALIPIA------REVFWGPMA 982
+ ++ +EG +A + A + R+RPI +T+ A L ++P+A +
Sbjct: 945 VEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQN-AVG 1003

Query: 983 YAMMGGIIAGTAITLLFLPALYV 1005
+MGG+++ T + + F+P +V
Sbjct: 1004 IGVMGGMVSATLLAIFFVPVFFV 1026



Score = 96.8 bits (241), Expect = 2e-22
Identities = 89/507 (17%), Positives = 173/507 (34%), Gaps = 34/507 (6%)

Query: 10 WALNHRSLVWYFMLVFLVAGLVSYLDLGREEDPEFTVKTMVVQANWP-GASIDET---LN 65
L + + +V +L L PE + P GA+ + T L+
Sbjct: 532 KILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLD 591

Query: 66 QVTDRLEKKLEELDTLDYTRS-VTTAGKSV----VFVFLKDTTRAQDVKKSWTEVRHLLT 120
QVTD K + +T + + +G++ FV LK + S V H
Sbjct: 592 QVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAK 651

Query: 121 DIQNTLPQGVYGPYFNDQFGDVFGNIYAFTADGLSMR-QLRDYAEDVRTKILTIPNAGKV 179
+ G P FN G F + + D R ++L +
Sbjct: 652 MELGKIRDGFVIP-FNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPA 710

Query: 180 ELIGAQDEA------IYLEFSTRQVAALGLDQQAILKALQDQNAITPSGVVQAGPE--RI 231
L+ + LE + ALG+ I + + T ++
Sbjct: 711 SLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKL 770

Query: 232 SVRVSGQF-NSEADLRAVNLRVNN-RFFRLSDVATITRGYVDPPTSIFRVNGEDAIGLGI 289
V+ +F D+ + +R N S T Y + R NG ++ +
Sbjct: 771 YVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVY--GSPRLERYNGLPSMEIQG 828

Query: 290 GMKPNGNLLEFGAELDKMMDQVTAELPVGVKVFKVADQPQVVKEAVSGFTQALFEAVVIV 349
P + + A ++ + + LP G+ + + + + + + V+V
Sbjct: 829 EAAPGTSSGDAMALMENLASK----LPAGIG-YDWTGMSYQERLSGNQAPALVAISFVVV 883

Query: 350 ---LAVSFVSLGLRAGFVVSLSIPLVLAITFLSMSLMDISLQRVSLGALIIALGLLVDDA 406
LA + S V L +PL + L+ +L + + L+ +GL +A
Sbjct: 884 FLCLAALYESW--SIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNA 941

Query: 407 MIAVEMMVARLEH-GDPINKAATYVYSHTAFPMLTGTLVTIAGFIPIGLNNSQAGEYTFT 465
++ VE +E G + +A P+L +L I G +P+ ++N
Sbjct: 942 ILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNA 1001

Query: 466 LFVVIAVSLLVSWVVAVLFAPLLGVTF 492
+ + + ++ + ++A+ F P+ V
Sbjct: 1002 VGIGVMGGMVSATLLAIFFVPVFFVVI 1028



Score = 73.3 bits (180), Expect = 4e-15
Identities = 81/524 (15%), Positives = 172/524 (32%), Gaps = 44/524 (8%)

Query: 517 LSMRHKWTTIITTIVLFVISVFGMGFIERQFFPQSDRPELVLDWTLPQNSSIADTKAQME 576
+R + I+L + + + +P P + + P + + +
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYP-GADAQTVQDTVT 62

Query: 577 RFEETMLKDNPDVEH-WSTYVGQGAIRFVLSFDVQPANPYFGQMVAVARDVEARDRLKAK 635
+ E + ++ + ST G++ L+F +P Q+ + A L +
Sbjct: 63 QVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSG-TDPDIAQVQVQNKLQLATPLLPQE 121

Query: 636 FEAAFRKDYVGTDVYVKYLELGPPVGRPVQYRISGPDVQKLRGIAQDFA--GILSADKRL 693
+ + V+ + + + D+ + RL
Sbjct: 122 VQQ--------QGISVEKSSSSYLM----VAGFVSDNPGTTQDDISDYVASNVKDTLSRL 169

Query: 694 -GVVNYNWNEPGRVIRVDVMQDKARKLGISSKDIATTLNG----VVGGITITQVRDSIYL 748
GV + +R+ + D K ++ D+ L + G
Sbjct: 170 NGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQ 229

Query: 749 IDVIARAQAHERDSIDTLQSLQVATGNGTSVPLAAIANFRYELEQPVIYRRSR-IPTITV 807
++ AQ ++ + + +G+ V L +A E + R P +
Sbjct: 230 LNASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGL 289

Query: 808 AAGIIDKTMPDTIARDLGPAIKTFADRLPAGYYIQ----TAGTVEESGKSQGPIAAVVPL 863
+ A+ + + P G + T V+ S I VV
Sbjct: 290 GIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLS------IHEVVKT 343

Query: 864 MLFVMATVLMVQ---LQSFQRLFLVVAVA-PLGLIGVVAALLPSGKPLGFVAILGVLALI 919
+ + V +V LQ+ R L+ +A P+ L+G A L G + + + G++ I
Sbjct: 344 LFEAIMLVFLVMYLFLQNM-RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAI 402

Query: 920 GILIRNSVILIVQVEEDIQE-GLHPWDAVMKASQHRMRPIALTAAAASLALIPIA----- 973
G+L+ ++++++ VE + E L P +A K+ + A S IP+A
Sbjct: 403 GLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGS 462

Query: 974 REVFWGPMAYAMMGGIIAGTAITLLFLPALYVAWFRIKEPEHSP 1017
+ + ++ + + L+ PAL + EH
Sbjct: 463 TGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHE 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0859RTXTOXIND612e-12 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 61.0 bits (148), Expect = 2e-12
Identities = 42/209 (20%), Positives = 77/209 (36%), Gaps = 11/209 (5%)

Query: 80 ERLVDIGQHVEKGQLLARIDDVEQRANMRVAQASMDAAEAQLVQADANFKRQQALLKQGF 139
RL D + K +A+ +EQ A + ++QL Q ++ + +
Sbjct: 235 SRLDDFSSLLHKQ-AIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQL-V 292

Query: 140 TTRSQYDQADQALRTAQSALSSAQSQLGNARDELSYTELHAPVSGVVTARNIET-GQVVQ 198
T + + D+ LR + +L + + + APVS V + T G VV
Sbjct: 293 TQLFKNEILDK-LRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVT 351

Query: 199 AAQTAFSIA-EDGSRDAIFDVQETLVNHARIGMGVTVALLADPSIEA---EGNIRELSP- 253
A+T I ED + + VQ + +G + + A P G ++ ++
Sbjct: 352 TAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLD 411

Query: 254 -VVDAQTGTV-RVKVGIAQATAQMALGAI 280
+ D + G V V + I + I
Sbjct: 412 AIEDQRLGLVFNVIISIEENCLSTGNKNI 440



Score = 45.2 bits (107), Expect = 3e-07
Identities = 21/130 (16%), Positives = 48/130 (36%), Gaps = 28/130 (21%)

Query: 74 VNGQVVERLVDIGQHVEKGQLLARIDDVEQRANMRVAQASMDAAEAQLVQ---------- 123
N V E +V G+ V KG +L ++ + A+ Q+S+ A + +
Sbjct: 103 ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIEL 162

Query: 124 -----------------ADANFKRQQALLKQGFTT-RSQYDQADQALRTAQSALSSAQSQ 165
++ R +L+K+ F+T ++Q Q + L ++ + ++
Sbjct: 163 NKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLAR 222

Query: 166 LGNARDELSY 175
+ +
Sbjct: 223 INRYENLSRV 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0863OUTRMMBRANEA280.030 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 28.4 bits (63), Expect = 0.030
Identities = 33/179 (18%), Positives = 58/179 (32%), Gaps = 45/179 (25%)

Query: 90 ASYGVGVGYRFNDMLRTDLTLDYF-RASINGRTNCPSYVKSSHGLNPVEDNCHYEDNSKA 148
G GY+ N + ++ D+ R G +Y + G+ +K
Sbjct: 56 LGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAY--KAQGVQL---------TAKL 104

Query: 149 SVWTAMANAYVDLPRVGPLTPYLGAGIGAAYVKYDTWKTSEICPTCTLQSDKDGFDSWRF 208
P L Y +G + DT +S+ G +
Sbjct: 105 G-----------YPITDDLDIY--TRLGGMVWRADT------------KSNVYGKNHDTG 139

Query: 209 AMALMA-GVSYDLTDQLKLDLGYRYLRVNGGNAYGYDEQDRQ------VINQYGQGAGA 260
+ A GV Y +T ++ L Y++ N G+A+ + V ++GQG A
Sbjct: 140 VSPVFAGGVEYAITPEIATRLEYQWTN-NIGDAHTIGTRPDNGMLSLGVSYRFGQGEAA 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A0865ALARACEMASE2517e-83 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 251 bits (643), Expect = 7e-83
Identities = 106/369 (28%), Positives = 168/369 (45%), Gaps = 22/369 (5%)

Query: 19 TIDLAALRHNYSAIATRIAPTRTAAVVKADAYGLGASRVAPAFYEAGCRDFFVAHLGEAV 78
++DL AL+ N S + R +VVKA+AYG G R+ A F + +L EA+
Sbjct: 8 SLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDG--FALLNLEEAI 65

Query: 79 ALKPFLKPDATLYVLNGLQPGTEAACAREG-ILPVLNSLEQVENWAALATRLGKKLPALL 137
L+ L +L G + + + ++S Q++ A RL L L
Sbjct: 66 TLRERGWKGPIL-MLEGFFHAQDLEIYDQHRLTTCVHSNWQLK--ALQNARLKAPLDIYL 122

Query: 138 QFDTGMSRLGLSAKEFDRLLENVTLLSRIDIKFAISHLANGDEPGNAANARQLAKMTALL 197
+ ++GM+RLG + + + ++ + +SH A + P + +A++
Sbjct: 123 KVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISG--AMARIEQAA 180

Query: 198 ARLPKLPAALANSGGTFLGKTYYFDLARPGIALYGIDPERQHDFSDKVAHENKKPKHSIL 257
L +L+NS T +FD RPGI LYG P + + + ++ L
Sbjct: 181 EGLE-CRRSLSNSAATLWHPEAHFDWVRPGIILYGASP----------SGQWRDIANTGL 229

Query: 258 -PVLPLSARVIQVRDVDKGATVGYGGTYVANGPMRIATIAVGYADGLFRSLSNKGAAFFG 316
PV+ LS+ +I V+ + G VGYGG Y A RI +A GYADG R
Sbjct: 230 RPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVD 289

Query: 317 DTRLPIIGRVSMDSITLDVTSLPEGTLKLGSLVELIGPHQRLEDVARDCDTIPYEILTAL 376
R +G VSMD + +D+T P+ +G+ VEL G +++DVA T+ YE++ AL
Sbjct: 290 GVRTMTVGTVSMDMLAVDLTPCPQA--GIGTPVELWGKEIKIDDVAAAAGTVGYELMCAL 347

Query: 377 GNRYARVYV 385
R V V
Sbjct: 348 ALRVPVVTV 356


21BOV_A1034BOV_A1058N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BOV_A1034218-0.963690hypothetical protein
BOV_A1035218-0.928077hypothetical protein
BOV_A1036318-1.207105flagellar biosynthesis protein FliR
BOV_A1037117-1.151086flagellar biosynthesis protein FlhA
BOV_A1038116-1.217554flagellar biosynthesis protein FliQ
BOV_A1039115-0.985515flagellar basal body rod modification protein
BOV_A1040116-1.368370flagellar biosynthesis repressor FlbT
BOV_A1041-215-0.428383flagellar biosynthesis regulatory protein FlaF
BOV_A1042-2150.057757flagellar hook-associated protein FlgL
BOV_A1043-2160.504736flagellar hook-associated protein FlgK
BOV_A1044-2150.893554flagellar hook protein FlgE
BOV_A10450170.489624response regulator receiver
BOV_A1047315-0.303064motD-related protein
BOV_A1048315-0.737550chemotaxis protein
BOV_A1050416-1.058703hypothetical protein
BOV_A1051416-1.201903flagellar MS-ring protein
BOV_A1052517-1.299951flagellin family protein
BOV_A1053516-1.007896outer membrane autotransporter
BOV_A105409-0.247582hypothetical protein
BOV_A1057090.184544D-xylose ABC transporter permease
BOV_A10581121.180423AraC family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1034FLGFLGJ320.001 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 32.0 bits (72), Expect = 0.001
Identities = 15/64 (23%), Positives = 29/64 (45%)

Query: 88 SPAYRKFEAFMLQSFVQSMFTSDTTATFGKGIAGEYWKSMMAEAMANKMADGGGVGIARL 147
P R+ E +Q ++SM + + SM + +A +M G G+G+A +
Sbjct: 34 RPVARQVEGMFVQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEM 93

Query: 148 LEEQ 151
+ +Q
Sbjct: 94 MVKQ 97


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1036TYPE3IMRPROT825e-21 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 82.5 bits (204), Expect = 5e-21
Identities = 51/203 (25%), Positives = 92/203 (45%), Gaps = 4/203 (1%)

Query: 17 LAFCRIGACLMLMPGISSARIPLQVRLFIALACSFAVLPLVLQRIVTAVDAGQPWTLFRL 76
R+ A + P +S +P +V+L +A+ +FA+ P + V W L
Sbjct: 18 WPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPANDVPVFSFFALW----L 73

Query: 77 MVGEMFIGALIGILAHIYFWAQQFMANMMAMAVGYSGSPADSITENEPQATLATIVTFSA 136
V ++ IG +G F A + ++ + +G S + + LA I+ A
Sbjct: 74 AVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIMDMLA 133

Query: 137 LFLFFVTDMHLEIFRALLNSYVAIPVDGRFRPDAAMIDFTDALSAAFLATLRIAAPFIVF 196
L LF + HL + L++++ +P+ G A + T A S FL L +A P I
Sbjct: 134 LLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLALPLITL 193

Query: 197 AILVNFAIGLVNKLTPAIPVYFI 219
+ +N A+GL+N++ P + ++ I
Sbjct: 194 LLTLNLALGLLNRMAPQLSIFVI 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1037GPOSANCHOR357e-04 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 35.4 bits (81), Expect = 7e-04
Identities = 20/53 (37%), Positives = 29/53 (54%)

Query: 309 VGIAVPRRQARQREADAAEAGKKQREAEEQERNSVKASLETNQIELCLGKQLS 361
I+ RQ+ +R+ DA+ KKQ E +E NS A+LE EL K+L+
Sbjct: 374 NKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLT 426



Score = 32.7 bits (74), Expect = 0.005
Identities = 18/55 (32%), Positives = 27/55 (49%), Gaps = 6/55 (10%)

Query: 316 RQARQREADAAEAGKKQREAEEQERNSVKASLETNQIELCLGKQLSARLIASQEE 370
RQ+ +R+ DA+ KKQ EAE Q+ E ++ + L L AS+E
Sbjct: 346 RQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASR------QSLRRDLDASREA 394


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1038TYPE3IMQPROT571e-14 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 56.7 bits (137), Expect = 1e-14
Identities = 23/73 (31%), Positives = 41/73 (56%)

Query: 5 DALDIVNSAIWTVLTASGPAVLAAMLAGIGIALFQALTQIQEMTLTFVPKIIVIFVVLAL 64
D + N A++ VL SG + A + G+ + LFQ +TQ+QE TL F K++ + + L L
Sbjct: 3 DLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFL 62

Query: 65 TAPFVGAQINAFT 77
+ + G + ++
Sbjct: 63 LSGWYGEVLLSYG 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1043FLGHOOKAP1821e-18 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 81.5 bits (201), Expect = 1e-18
Identities = 105/554 (18%), Positives = 192/554 (34%), Gaps = 105/554 (18%)

Query: 4 SSALLTAKSSLAATSKQTSVVSRNISGAKDADYSRRT---ASLVSGPYGSLYVG------ 54
SS + A S L A + S NIS A Y+R+T A S +VG
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVS 60

Query: 55 -ISRSADEAMFNRYIQSNSAASASSTLADGLDRLSALYSADNYSGSPSGLIGDLRDALQT 113
+ R D + N+ + + +S + + + ++ + S + S + + D +LQT
Sbjct: 61 GVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS--TSSLATQMQDFFTSLQT 118

Query: 114 YAASPSNSALGDSVVSVAQSLRND--------------ADREIADSVANINDLLAKFEKA 159
++ + A +++ ++ L N + I SV IN+ +
Sbjct: 119 LVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASL 178

Query: 160 NQDV--VGGTRMGRDVSDYLDQRDALLKQLSGEIGITTMMRGDNDMVIFAENGVTLFETT 217
N + + G G ++ LDQRD L+ +L+ +G+ ++ I NG +L + +
Sbjct: 179 NDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGS 238

Query: 218 ARKVTFEQSAVLTPGVAGKAVT----VDGVPLSHDTFDQPFGTGRLSGLLQLRDQM---- 269
T Q A + P A + T VDG + + ++ TG L G+L R Q
Sbjct: 239 ----TARQLAAV-PSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQT 293

Query: 270 --QLDEIARGLVTVFAESDQTGSSPDQ---TGLFSWSGSPAIPGADLSAGIAGTIEVSVP 324
L ++A F + G + F+ G PA+ + G
Sbjct: 294 RNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFA-IGKPAVLQNTKNKGDVAIGATVTD 352

Query: 325 FIASEGGSALLLRDGGANGANYK--------YNVQGAAGFSDRLR-------ALNEAFSE 369
A + D D L A+N++F+
Sbjct: 353 ASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTL 412

Query: 370 PMVFDAAAGI-----------------------SSSSSLIGYSASSLGWLEGKRQKANSE 406
V DA + + +L+ ++S G + N
Sbjct: 413 KPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQALLDLQSNSK--TVGGAKSFNDA 470

Query: 407 F-----------TYNGTVASQADFALSNAT-------GVDIDTEMALLLDLEHSYQASSR 448
+ T ++ ++ + GV++D E L + Y A+++
Sbjct: 471 YASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQ 530

Query: 449 VLTTVSAMLDDLLN 462
VL T +A+ D L+N
Sbjct: 531 VLQTANAIFDALIN 544


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1044FLGHOOKAP1415e-06 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 41.1 bits (96), Expect = 5e-06
Identities = 12/48 (25%), Positives = 27/48 (56%)

Query: 329 IMSGTLEESNADIAQELTDMIEAQRSYTANSKVFQTGSELMDVLVNLK 376
+ + S ++ +E ++ Q+ Y AN++V QT + + D L+N++
Sbjct: 499 LSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 34.2 bits (78), Expect = 8e-04
Identities = 12/33 (36%), Positives = 20/33 (60%)

Query: 9 TGVSGMNAQANRLSTVADNIANASTVGYKRAET 41
+SG+NA L+T ++NI++ + GY R T
Sbjct: 6 NAMSGLNAAQAALNTASNNISSYNVAGYTRQTT 38


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1045HTHFIS320.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.7 bits (72), Expect = 0.002
Identities = 25/148 (16%), Positives = 53/148 (35%), Gaps = 10/148 (6%)

Query: 2 IVVVDDRDMVTEGYSSWFGREGITTTGF-TPTDFDEWVESVPEQDIMAIEAFLIGECADQ 60
I+V DD + + R G W+ + D++ + + E +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD-GDLVVTDVVMPDE--NA 62

Query: 61 HRLPARIRER-CKAPVIAVNDRPSLEHTLELFQFGVDDVVRKPVHVREILARI-NAIRRR 118
L RI++ PV+ ++ + + ++ + G D + KP + E++ I A+
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 119 AGASATSGADGTQLGPIRVFSDGRDPQI 146
+ D P+ GR +
Sbjct: 123 KRRPSKLEDDSQDGMPLV----GRSAAM 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1047FLGHOOKFLIK347e-04 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 34.4 bits (78), Expect = 7e-04
Identities = 28/129 (21%), Positives = 52/129 (40%), Gaps = 15/129 (11%)

Query: 257 KTLQIRLDPVELGTVTARIRVAGDSVEAHLVADKSHAAEMLAADRSMIEKALKVAGVGDD 316
++ ++RL P +LG V ++V + + +V+ H L A ++ L +G+
Sbjct: 257 QSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGI--- 313

Query: 317 TKISVTVADRNAQGAAQHVAAAQNAGQQQASAQQQGHQLAFNMQQQGSQGRGGEAQAQFM 376
Q +++ +GQQQA++QQQ Q N + + +
Sbjct: 314 ------------QLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSL 361

Query: 377 SGRSGGEGG 385
GR G G
Sbjct: 362 QGRVTGNSG 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1051FLGMRINGFLIF5380.0 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 538 bits (1386), Expect = 0.0
Identities = 159/576 (27%), Positives = 252/576 (43%), Gaps = 76/576 (13%)

Query: 9 LGKLGARKLIALGLVGAALMGAILYTSIYLGRPSYETLYVGLSRDDVNRMGLALGEAGIP 68
L +L A I L + G+A + ++ ++ P Y TL+ LS D + L + IP
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 69 FDVKSDGSSILVPIGKAENARMYLAEKGLPTSNNAGYELFDNMGSLGLTSFMQEITRVRA 128
+ + +I VP K R+ LA++GLP G+EL D G++ F +++ RA
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQE-KFGISQFSEQVNYQRA 134

Query: 129 LEGEIARTIQAIRGVKAARVHIVLAEKGSFRRGDQKPSASVVIRAEGGFSAESAQ--SIR 186
LEGE+ARTI+ + VK+ARVH+ + + F R + PSASV + E G + + Q ++
Sbjct: 135 LEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVV 194

Query: 187 QLVAAAVPSLDASSVTVLDTNGHLLA---SAGEGANGAALMTASLEQQVASHVDDSIRKA 243
LV++AV L +VT++D +GHLL ++G N A L A+ V S + I
Sbjct: 195 HLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAN---DVESRIQRRIEAI 251

Query: 244 LAPYLGLGHFQTSVQAALDTDRRQTKETTYDPESRVERSVRVVRESGDSRNNRNDNATGV 303
L+P +G G+ V A LD ++ E Y P ++ R+ S GV
Sbjct: 252 LSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGV 311

Query: 304 -------------------------EQNIPQEQIQNRNGESSTEKTDRREELTNYEVNSK 338
QN PQ N S+ ++ +R E +NYEV+
Sbjct: 312 PGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTST-NSNSAGPRSTQRNETSNYEVDRT 370

Query: 339 TVSTVSDGYSIKRLSIAVVIDQARLLQTAGTTPPPANFVDQQITKIRDLVATAAGLNTNR 398
T + I+RLS+AVV++ L A P P Q+ +I DL A G + R
Sbjct: 371 IRHTKMNVGDIERLSVAVVVNYKTL---ADGKPLP--LTADQMKQIEDLTREAMGFSDKR 425

Query: 399 GDVINVTAVNF--LDPAGADMEPVSAPW--TDTLLRQSGSYANALAILAAVGLLIWFGLR 454
GD +NV F +D G ++ P+ + + Q + L +L +L +R
Sbjct: 426 GDTLNVVNSPFSAVDNTGGEL-----PFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVR 480

Query: 455 PLL--RDQNVKPAGTEVAIREAGEVATPNFIGGAESVGEGVQAVIGGPAAYADQMKTSLS 512
P L R + K A + +R+ E A + E L
Sbjct: 481 PQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDE----------------------QLQ 518

Query: 513 DLRQRMRMPAKL---RLEQMIEMDEERVAAVLKQWI 545
R R+ A++ R+ +M + D VA V++QW+
Sbjct: 519 QRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWM 554


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1052FLAGELLIN805e-19 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 79.7 bits (196), Expect = 5e-19
Identities = 45/227 (19%), Positives = 83/227 (36%), Gaps = 7/227 (3%)

Query: 4 ILTNSSALTALQTLASTNKSLESTQNRISTGLRISEASDNASYWSIATSMKSDNKANSAV 63
I TNS +L L + SL S R+S+GLRI+ A D+A+ +IA S+ K +
Sbjct: 4 INTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQA 63

Query: 64 QDALGLGAGKVDTAYSAINKIRESVDDIKTKLVSAMGA--STEDKGKIETEIKSIVANIN 121
G T A+N+I ++ ++ V A S D I+ EI+ + I+
Sbjct: 64 SRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEID 123

Query: 122 SALSNANYAGSNLLNGPTTDLNVVASYNRSGNAVTVDKITVKATDTDAKTMVKDIVDAGF 181
+ + G +L+ V + N I ++ D + + V+
Sbjct: 124 RVSNQTQFNGVKVLSQDNQMKIQVGA-----NDGETITIDLQKIDVKSLGLDGFNVNGPK 178

Query: 182 FTSASDDTAIGTALNTVETALASLATGAATLGAAKSQIDSQKSFLSA 228
+ D + + +T + + D+ +
Sbjct: 179 EATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPD 225


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1053PERTACTIN602e-10 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 59.7 bits (144), Expect = 2e-10
Identities = 88/384 (22%), Positives = 134/384 (34%), Gaps = 45/384 (11%)

Query: 2979 DTLLTNRLVIDGDTAGTTQVSVTNRGGLGAQTVEGIKIIQVGGASNGMFLLAGDYMFNGE 3038
D L+++LV+ D +G ++ V N G + + ++Q S F LA N +
Sbjct: 487 DLGLSDKLVVMRDASGQHRLWVRNSGS-EPASGNTMLLVQTPRGSAATFTLA-----NKD 540

Query: 3039 QAVVAGAYGYRLYKGGVSTPADGNWYLRSALL----NPETPTNPTDPETPLYQPGVPLYE 3094
V G Y YRL G +G W L A P P P P P
Sbjct: 541 GKVDIGTYRYRLAANG-----NGQWSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPP 595

Query: 3095 SYAGSLQQLNKLGTLQQRVGNRVWAKHPVPAQNDENGAGPS------------------- 3135
Q+ + Q G + A G +
Sbjct: 596 QPPQPPQRQPEAPAPQPPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRLN 655

Query: 3136 -GNNGIWARIEAAHAEFDPKQSTSRASYDADIWKFQTGIDGMFAETASGKFIGGVYVQYG 3194
G W R A + D + +D + F+ G D A +GG+ G
Sbjct: 656 PDAGGAWGRGFAQRQQLDNRAGRR---FDQKVAGFELGADHAVAVAGGRWHLGGLA---G 709

Query: 3195 TVSSSVSSPFGNGSIESSGYGAGATLTWYGESDFYIDGVAQINWFDSDLNSATLGRQLVD 3254
G+G + G T+ S FY+D + + ++D A V
Sbjct: 710 YTRGDRGFT-GDGGGHTDSVHVGGYATYIANSGFYLDATLRASRLENDFKVAGSDGYAVK 768

Query: 3255 GN-RAVGYSLSVETGQKIEIGEGWSLTPQAQLAYSAIRFDDFKDAFDTSVSPENDHDLTG 3313
G R G +S+E G++ +GW L PQA+LA + ++ A V E + G
Sbjct: 769 GKYRTHGVGVSLEAGRRFAHADGWFLEPQAELAVFRVGGGAYRAANGLRVRDEGGSSVLG 828

Query: 3314 RLGLAINRDAEWLDAQGRRVAMHI 3337
RLGL + + E A GR+V +I
Sbjct: 829 RLGLEVGKRIEL--AGGRQVQPYI 850


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BOV_A1058HTHFIS348e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 8e-04
Identities = 21/97 (21%), Positives = 37/97 (38%), Gaps = 2/97 (2%)

Query: 163 RKLSAHDPMAHMLHALIAKIMATTESDSASQHLRQLMFNATRQAFHSTGESAPLHSLHDS 222
++ + + + + S S + Q + RQ F S G++ P L+D
Sbjct: 372 DVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDR 431

Query: 223 ASKRLQIVDLLIDRHLTETELSARWLATRLGISIRTL 259
++ LI LT T + A LG++ TL
Sbjct: 432 VLAEME--YPLILAALTATRGNQIKAADLLGLNRNTL 466



 
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