PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genome2236.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_008577 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1Shewana3_0095Shewana3_0106Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0095-1143.369594hypothetical protein
Shewana3_0096-2163.987583MarR family transcriptional regulator
Shewana3_0097-2184.162875FAD-binding 9, siderophore-interacting
Shewana3_0098-1224.251441imidazolonepropionase
Shewana3_00990224.841622histidine utilization repressor
Shewana3_0100-1225.121753urocanate hydratase
Shewana3_0101-1195.224159histidine ammonia-lyase
Shewana3_01030194.929132formate dehydrogenase subunit beta
Shewana3_0104-2184.544742formate dehydrogenase subunit gamma
Shewana3_0105-1194.693935formate dehydrogenase accessory protein FdhE
Shewana3_0106-1163.846065selenocysteine synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0097TOXICSSTOXIN280.021 Staphylococcal toxic shock syndrome toxin signature.
		>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature.

Length = 234

Score = 28.5 bits (63), Expect = 0.021
Identities = 12/30 (40%), Positives = 16/30 (53%)

Query: 205 LRKLLKQTYDLPKSHFYTSSYWKIGCNEGE 234
+R L Q + L +S T YWKI N+G
Sbjct: 173 IRHQLTQIHGLYRSSDKTGGYWKITMNDGS 202


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0098UREASE441e-06 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 43.6 bits (103), Expect = 1e-06
Identities = 23/56 (41%), Positives = 31/56 (55%), Gaps = 8/56 (14%)

Query: 348 LAGLTLNAAKALGIEESVGSLVVGKQADFCLWDIATPAQLAYSYGVNPCKDVVKNG 403
+A T+N A A G+ +GSL VGK+AD LW+ PA +GV P V+ G
Sbjct: 406 IAKYTINPAIAHGLSHEIGSLEVGKRADLVLWN---PAF----FGVKP-DMVLLGG 453



Score = 31.6 bits (72), Expect = 0.007
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 6/61 (9%)

Query: 23 YGAITNAAIAVKDGKIAWLGPRSE---LPAFDVL---SIPVYRGKGGWITPGLIDAHTHL 76
+ I A I +KDG+IA +G P ++ V G+G +T G +D+H H
Sbjct: 80 HWGIVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEGKIVTAGGMDSHIHF 139

Query: 77 V 77
+
Sbjct: 140 I 140


2Shewana3_0159Shewana3_0168Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_01592130.346131general secretion pathway protein L
Shewana3_0160317-0.788042general secretion pathway M protein
Shewana3_0161318-1.432618type II secretion system protein N
Shewana3_0162322-1.037209methyl-accepting chemotaxis sensory transducer
Shewana3_01635191.637234aspartyl/asparaginyl beta-hydroxylase
Shewana3_01645201.585413phytanoyl-CoA dioxygenase
Shewana3_01656192.035774hypothetical protein
Shewana3_01666202.424870N-acetyltransferase GCN5
Shewana3_01676202.546181phage tail collar domain-containing protein
Shewana3_01685182.288896Ig family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0166SACTRNSFRASE436e-08 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 43.4 bits (102), Expect = 6e-08
Identities = 21/93 (22%), Positives = 42/93 (45%), Gaps = 3/93 (3%)

Query: 57 QQVSYREQYPHAISYILFYQQQAVGKLMLDLNEHRVHLVDFI-ITPSMRGRGLGSAVLEA 115
VSY E+ A ++ + + +G++ + N + L++ I + R +G+G+A+L
Sbjct: 55 MDVSYVEEEGKAA-FLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHK 113

Query: 116 VKLEASQRQLP-VHLSVESENTQAKSLYLRHGF 147
A + + L + N A Y +H F
Sbjct: 114 AIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0168OMPADOMAIN456e-06 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 44.5 bits (105), Expect = 6e-06
Identities = 40/189 (21%), Positives = 57/189 (30%), Gaps = 42/189 (22%)

Query: 3348 ALGSLLLLSVTQQA-QATDWYVEGFIGQAQADKTLPELNVQVGEGQLINVDDSDTAFGVS 3406
A+ +V Q A + WY +G +Q T N ++ G
Sbjct: 9 AVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTH-------ENQLGAGAF 61

Query: 3407 LGYQWTPTVALELGYADFG---------EGSAKIKGATLTPEQYHELVKTVTPVLADGVM 3457
GYQ P V E+GY G G+ K +G LT K P+ D +
Sbjct: 62 GGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA-------KLGYPITDDLDI 114

Query: 3458 LGLRFTLLQHQGWRFEVPVGLFHWQADISSTMGNTRIKTDLDGTDWYAGVRFSYQFSDAW 3517
+G W+AD T N K G Y +
Sbjct: 115 YT---------------RLGGMVWRAD---TKSNVYGKNHDTGVSPVFAGGVEYAITPEI 156

Query: 3518 SVGLGYQYI 3526
+ L YQ+
Sbjct: 157 ATRLEYQWT 165


3Shewana3_0296Shewana3_0343Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_02962271.039047hypothetical protein
Shewana3_02971241.163968sodium:dicarboxylate symporter
Shewana3_02982271.508527hypothetical protein
Shewana3_02992271.566837TonB-dependent siderophore receptor
Shewana3_03004351.070761glutamine synthetase
Shewana3_0301-1172.404667GTP-binding protein TypA
Shewana3_0302-2103.016722AraC family transcriptional regulator
Shewana3_0303-1112.542324short-chain dehydrogenase/reductase SDR
Shewana3_0304-2122.242909hypothetical protein
Shewana3_0305-2132.388139alcohol dehydrogenase
Shewana3_0306-1172.394431catalase/peroxidase HPI
Shewana3_03071151.3891434Fe-4S ferredoxin
Shewana3_03080161.208979diguanylate cyclase
Shewana3_0309-1171.422551hypothetical protein
Shewana3_03102201.454421hypothetical protein
Shewana3_03111171.088479ribonuclease BN
Shewana3_03121161.317259prolyl aminopeptidase
Shewana3_03132211.492567hypothetical protein
Shewana3_03141221.755103D-tyrosyl-tRNA(Tyr) deacylase
Shewana3_03151182.696240thioesterase domain-containing protein
Shewana3_03160172.837508azoreductase
Shewana3_03172182.257327hypothetical protein
Shewana3_03182171.315629rhodanese domain-containing protein
Shewana3_03192180.147538XRE family transcriptional regulator
Shewana3_0320315-1.163393benzoate transporter
Shewana3_0321016-0.769662N-acetyltransferase GCN5
Shewana3_0322116-0.127839antibiotic acetyltransferase
Shewana3_03230180.996115hypothetical protein
Shewana3_03241192.632582hypothetical protein
Shewana3_03250193.543061hypothetical protein
Shewana3_0326-1194.3392393-oxoacyl-(acyl carrier protein) synthase II
Shewana3_03270193.9037473-ketoacyl-ACP reductase
Shewana3_03281193.693974thioester dehydrase family protein
Shewana3_03292183.3886333-oxoacyl-ACP synthase
Shewana3_03301153.298162hypothetical protein
Shewana3_03310183.755346FAD-binding monooxygenase
Shewana3_03321203.851242hypothetical protein
Shewana3_0333-1213.956195hypothetical protein
Shewana3_0334-2224.0390604-hydroxybenzoyl-CoA thioesterase
Shewana3_0335-2213.698459histidine ammonia-lyase
Shewana3_0336-1212.113769glycosyl transferase family protein
Shewana3_03371200.700363thioester dehydrase family protein
Shewana3_03381200.033193aconitate hydratase
Shewana3_0339220-2.867906hypothetical protein
Shewana3_0340-220-1.512130transposase
Shewana3_0341-1180.491454integrase catalytic subunit
Shewana3_03420211.793592hypothetical protein
Shewana3_03430223.075040acyl carrier protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0299V8PROTEASE310.013 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 31.1 bits (70), Expect = 0.013
Identities = 17/119 (14%), Positives = 34/119 (28%), Gaps = 12/119 (10%)

Query: 608 TNLHLVRSEVKDEDRWNDETVTYASPSKATAFIGWRGETQKIRLQAEHSFSAESD---YR 664
TN H+V + D + S + ++I +S E D +
Sbjct: 116 TNKHVVDATHGDPHA----LKAFPSAINQDNYPNGGFTAEQI-----TKYSGEGDLAIVK 166

Query: 665 FKTDDGLASELDSYTTLDLLGSIDLPVGTLSYGIENLLDKDYSTLWGQRAVYFYSPTYG 723
F ++ + + + + V DK +T+W + Y
Sbjct: 167 FSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEA 225


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0301TCRTETOQM1753e-49 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 175 bits (444), Expect = 3e-49
Identities = 102/447 (22%), Positives = 173/447 (38%), Gaps = 87/447 (19%)

Query: 4 NLRNIAIIAHVDHGKTTLVDKLLSQSGTLATRGEATE--RVMDSNDLEKERGITILAKNT 61
+ NI ++AHVD GKTTL + LL SG + G + D+ LE++RGITI T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 62 AIKWNDYRINIVDTPGHADFGGEVERVLSMVDSVLLLVDAVDGPMPQTRFVTKKAFAQGL 121
+ +W + ++NI+DTPGH DF EV R LS++D +LL+ A DG QTR + G+
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 122 KPIVVINKIDRPGARPDWVIDQVFD-------------LFDNLGATDEQLD--------- 159
I INKID+ G V + + L+ N+ T+
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 160 --------------------------------FPVVYASALNGFATLDPDEVGTDMTPLF 187
FPV + SA N +G D L
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNN--------IGID--NLI 231

Query: 188 QTIVEKVSSPAADAEGPFQMQISQLDYNSYVGVIGVGRIKRGSIKTNQQVTVIGADGKTR 247
+ I K S + ++ +++Y+ + R+ G + V + +
Sbjct: 232 EVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKI 291

Query: 248 NGKMGQVLGYMGLERTEVDVANAGDIVAITGLGELKISDTVCAAGNVEALPP---LSVDE 304
+ G E ++D A +G+IV + LK++ + G+ + LP +
Sbjct: 292 TEMYTSING----ELCKIDKAYSGEIVILQNEF-LKLNSVL---GDTKLLPQRERIENPL 343

Query: 305 PTLTMTFQVNTSPFAGKEGKYVTSRNILERLQQELVHNVALRVEETDSPDRFAVSGRGEL 364
P L T + + +L+ L + + LR + +S G++
Sbjct: 344 PLLQTTVEPSKPQQREM---------LLDALLEISDSDPLLRYYVDSATHEIILSFLGKV 394

Query: 365 HLSILIENMRRE-GYELAVSRPEVILK 390
+ + ++ + E+ + P VI
Sbjct: 395 QMEVTCALLQEKYHVEIEIKEPTVIYM 421



Score = 37.5 bits (87), Expect = 1e-04
Identities = 20/89 (22%), Positives = 34/89 (38%), Gaps = 1/89 (1%)

Query: 389 LKTIDGELCEPYETLTVDVEEEHQGTVIEKLGVRKAEMKDMQLDGKGRVRVDFIIPSRGL 448
LK EL EPY + + +E+ A + D QL V + IP+R +
Sbjct: 528 LKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKN-NEVILSGEIPARCI 586

Query: 449 IGFQTEFLTATSGTGLIYHSFDHYGPHKG 477
++++ T+G + Y G
Sbjct: 587 QEYRSDLTFFTNGRSVCLTELKGYHVTTG 615


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0303DHBDHDRGNASE1081e-30 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 108 bits (271), Expect = 1e-30
Identities = 73/240 (30%), Positives = 114/240 (47%), Gaps = 14/240 (5%)

Query: 6 KTAVVTGAAGGIGQAIIRKLLAEGARVVAADLSADALKVFNDFPADKLVT---FSVDVTD 62
K A +TGAA GIG+A+ R L ++GA + A D + + L+ + F DV D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 63 YKQVEAMVKHAADQFGQLDILINNAGIGLAKPLLQHDPINDFEPVTNVNQKGVYHGILAG 122
++ + + G +DIL+N AG+ L L+ ++E +VN GV++ +
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGV-LRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 123 GRQFQAQGSRGVILNTSSVYAQIASEMTFTYNVSKAAVDMMTKCAALELAPLGVRVCAVA 182
+ + S G I+ S A + Y SKAA M TKC LELA +R V+
Sbjct: 128 SKYMMDRRS-GSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 183 PGRVDTPMLRQY--EALGLWEHIR--KEQMR-----QEFTQPNEIADVVAFLVSDEANCI 233
PG +T M + G + I+ E + ++ +P++IAD V FLVS +A I
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0321SACTRNSFRASE361e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.5 bits (84), Expect = 1e-05
Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 2/65 (3%)

Query: 69 EHAEIKSMRTAATYKQQGIASKVLQHLINDAKAAGVQRLSLETGSMAFFQPARNLYAKFG 128
+A I+ + A Y+++G+ + +L I AK L LET + A + YAK
Sbjct: 88 GYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINI--SACHFYAKHH 145

Query: 129 FELCG 133
F +
Sbjct: 146 FIIGA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0327DHBDHDRGNASE1065e-30 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 106 bits (266), Expect = 5e-30
Identities = 71/248 (28%), Positives = 114/248 (45%), Gaps = 15/248 (6%)

Query: 5 VLVTGSSRGIGKAIALKLAAAGYDIALHYHSNQAAADASAAELSALGVNVSLLKFDVADR 64
+TG+++GIG+A+A LA+ G IA N + + L A + DV D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPADVRDS 69

Query: 65 AAVKAAIEADIEANGAYYGVILNAGINRDNAFPAMSEAEWDSVIHTNLDGFYNVIHPCVM 124
AA+ G ++ AG+ R ++S+ EW++ N G +N V
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS-VS 128

Query: 125 PMVQGRKGGRIITLASVSGIAGNRGQVNYSASKAGLIGATKALSLELAKRKITVNCIAPG 184
+ R+ G I+T+ S Y++SKA + TK L LELA+ I N ++PG
Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188

Query: 185 LIETDM----------VADIPKDMVEQL---VPMRRMGKPNEIAALAAFLMSDDAAYITR 231
ETDM + K +E +P++++ KP++IA FL+S A +IT
Sbjct: 189 STETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITM 248

Query: 232 QVISVNGG 239
+ V+GG
Sbjct: 249 HNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0330FLGFLGJ290.023 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 28.5 bits (63), Expect = 0.023
Identities = 21/71 (29%), Positives = 30/71 (42%), Gaps = 3/71 (4%)

Query: 72 PKIQGNSQQGDSKAGVSSPQSFSQKVSIKVGDNTHELLTQLELE---GERMTLVGLAPLG 128
P+ +S GDSKA ++ +Q S + G H +L Q LE G+R
Sbjct: 138 PRNYDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPS 197

Query: 129 QALFTLVYDGN 139
LF + GN
Sbjct: 198 YNLFGVKASGN 208


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0332ACRIFLAVINRP396e-05 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 39.4 bits (92), Expect = 6e-05
Identities = 33/152 (21%), Positives = 58/152 (38%), Gaps = 23/152 (15%)

Query: 691 TLKLLGLALVIALLLFSLSFGVKRAALVV--AVPALAALLTLAILGLAGSPLSLFHALAL 748
+K L A+++ L+ L RA L+ AVP + L T AIL G ++ +
Sbjct: 340 VVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVP-VVLLGTFAILAAFGYSINTLTMFGM 398

Query: 749 ILVFGIGIDYS---------------LFFASAAQHG-KAVMMAVFMSACSTLLAFGLLAF 792
+L G+ +D + L A + + A+ A F +AF
Sbjct: 399 VLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAF 458

Query: 793 SQTQA---IHYFGLTLSLGIGFTFVLSPLILT 821
F +T+ + + +++ LILT
Sbjct: 459 FGGSTGAIYRQFSITIVSAMALSVLVA-LILT 489



Score = 32.1 bits (73), Expect = 0.010
Identities = 22/117 (18%), Positives = 43/117 (36%), Gaps = 17/117 (14%)

Query: 694 LLGLA-LVIALLLFSLSFGVKRAALVVAVPALAALLTLAILGLAGSPLSLFHALALILVF 752
L+ ++ +V+ L L +L V+ V L + L L ++ + L+
Sbjct: 875 LVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTI 934

Query: 753 GIGIDYSLFFASAA-----QHGKAVMMAV-----------FMSACSTLLAFGLLAFS 793
G+ ++ A + GK V+ A M++ + +L LA S
Sbjct: 935 GLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAIS 991


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0343FRAGILYSIN260.015 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 26.2 bits (57), Expect = 0.015
Identities = 20/74 (27%), Positives = 36/74 (48%), Gaps = 4/74 (5%)

Query: 1 MQNREQILAMLTTILVDEFEIDADAITTEAN--LYQELDLDSIDAVDLVIKLQQL--TGK 56
M+N + +L + T L+ +AD++TT + + +DL S+ DL +L + GK
Sbjct: 9 MKNVKLLLMLGTAALLAACSNEADSLTTSIDAPVTASIDLQSVSYTDLATQLNDVSDFGK 68

Query: 57 KIQPDEFKSVRTVN 70
I + R V+
Sbjct: 69 MIILKDNGFNRQVH 82


4Shewana3_0376Shewana3_0382Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0376017-3.652749****diguanylate cyclase/phosphodiesterase
Shewana3_037710234.335831hypothetical protein
Shewana3_037810224.276818OmpA/MotB domain-containing protein
Shewana3_03799224.264975TolC family type I secretion outer membrane
Shewana3_038010224.594509HlyD family type I secretion membrane fusion
Shewana3_038110245.208175ABC transporter-like protein
Shewana3_03829255.592984outer membrane adhesin like protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0378OMPADOMAIN885e-23 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 88.1 bits (218), Expect = 5e-23
Identities = 33/118 (27%), Positives = 53/118 (44%), Gaps = 12/118 (10%)

Query: 77 NVLFPNDSAYIAPEYYPQIEEIAMFLRQY--PTTKVTIEGHTSRTGTDERNAVLSQERAD 134
+VLF + A + PE ++++ L V + G+T R G+D N LS+ RA
Sbjct: 220 DVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQ 279

Query: 135 AVTAVLADRFSIDRSRLTAIGYGSSRPLVLEHTPDAETR---------NRRVVAEVTG 183
+V L + I +++A G G S P+ + + R +RRV EV G
Sbjct: 280 SVVDYLISK-GIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKG 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0380RTXTOXIND314e-104 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 314 bits (805), Expect = e-104
Identities = 92/432 (21%), Positives = 197/432 (45%), Gaps = 11/432 (2%)

Query: 29 RLIIWALAAMVACFLLWAGFAKLDKVTTGSGKVIPSSQVQVIQSLDGGIMQELFVREGDI 88
RL+ + + + + + +++ V T +GK+ S + + I+ ++ I++E+ V+EG+
Sbjct: 58 RLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGES 117

Query: 89 VTKGQPLVRIDDTRFRSDFAQQEQEVLGLKTNAIRMRAELDSILISDMTSDWREQVKITK 148
V KG L+++ +D + + +L + R + SI E K+ +
Sbjct: 118 VRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSI----------ELNKLPE 167

Query: 149 KALVFPDSITEAEPALVRRQQEEYNGRLDNLSNQLEILVRQIQQRQQEIDDLASKTTTLT 208
L V R + NQ + +++ E + ++
Sbjct: 168 LKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYE 227

Query: 209 TSMQLISRELELTRPLAKKGIVPEVELLKLERAVNDAQGELNSLRLLRPKLKAALDEAIL 268
++ L+ L K + + +L+ E +A EL + ++++ + A
Sbjct: 228 NLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKE 287

Query: 269 KRREAVFVYAADLRAQLNETQTRLSRMNEAQVGAQDKVSKAVITSPVNGTIKTVHINTLG 328
+ + ++ ++ +L +T + + +++ +VI +PV+ ++ + ++T G
Sbjct: 288 EYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEG 347

Query: 329 GVVQPGVDIIEIVPSEDQLLIETKILPKDIAFLHTGLPAVVKVTAYDFTRYGGLKGTVEH 388
GVV ++ IVP +D L + + KDI F++ G A++KV A+ +TRYG L G V++
Sbjct: 348 GVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKN 407

Query: 389 ISADTSQDDEGNSFYLIKVRTEESSLIKDDGTQMPIIPGMLTTVDVITGQRSILEYILNP 448
I+ D +D + + + EE+ L +P+ GM T ++ TG RS++ Y+L+P
Sbjct: 408 INLDAIEDQRLGLVFNVIISIEENCLS-TGNKNIPLSSGMAVTAEIKTGMRSVISYLLSP 466

Query: 449 ILRAKDTALRER 460
+ + +LRER
Sbjct: 467 LEESVTESLRER 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0382CABNDNGRPT913e-20 NodO calcium binding signature.
		>CABNDNGRPT#NodO calcium binding signature.

Length = 479

Score = 91.2 bits (226), Expect = 3e-20
Identities = 46/123 (37%), Positives = 60/123 (48%), Gaps = 2/123 (1%)

Query: 5622 HSDEFDQSGANDKADIILGGAGNDILFGQGGNDYLDGGAGKDTLYGGNGNDTLIGGAGND 5681
+D FD SG ++ I L + G GN + G + GG+GND L+G + ++
Sbjct: 300 GTDTFDFSGYSNNQRINLNEGSFSDVGGLKGNVSIAHGVTIENAIGGSGNDILVGNSADN 359

Query: 5682 TLIGGAGNDTLIGGLGDDVLRGDSGNDTFVWRYADADK--GTDHIMDFNVSEDKLDLSDL 5739
L GGAGND L GG G D L G +G DTFV+ D I DF DK+DLS
Sbjct: 360 ILQGGAGNDVLYGGAGADTLYGGAGRDTFVYGSGQDSTVAAYDWIADFQKGIDKIDLSAF 419

Query: 5740 LQG 5742

Sbjct: 420 RNE 422



Score = 53.1 bits (127), Expect = 3e-08
Identities = 29/90 (32%), Positives = 41/90 (45%), Gaps = 3/90 (3%)

Query: 4974 NGDFTHQPFNTGAKQTDASSGQDTVYGSNGNDHIVSTNGGGDHLLGYAGNDVLVGGDAIQ 5033
F ++ + V G GN I ++ +G +GND+LVG A
Sbjct: 301 TDTFDFSGYSNNQRINLNEGSFSDVGGLKGNVSIAHGVTI-ENAIGGSGNDILVGNSA-- 357

Query: 5034 GDTINGGTGNDILVAGLGQDSLYGGEGNDI 5063
+ + GG GND+L G G D+LYGG G D
Sbjct: 358 DNILQGGAGNDVLYGGAGADTLYGGAGRDT 387



Score = 49.2 bits (117), Expect = 5e-07
Identities = 20/80 (25%), Positives = 34/80 (42%), Gaps = 3/80 (3%)

Query: 4983 NTGAKQTDASSGQDTVYGSNGNDHIVSTNGGGDHLLGYAGNDVLVGGDAIQGDTINGGTG 5042
G ++ ++ + +G D L+G + +++L GG D + GG G
Sbjct: 319 EGSFSDVGGLKGNVSIAHGVTIENAIGGSGN-DILVGNSADNILQGGAG--NDVLYGGAG 375

Query: 5043 NDILVAGLGQDSLYGGEGND 5062
D L G G+D+ G G D
Sbjct: 376 ADTLYGGAGRDTFVYGSGQD 395



Score = 35.3 bits (81), Expect = 0.007
Identities = 17/82 (20%), Positives = 25/82 (30%), Gaps = 2/82 (2%)

Query: 4982 FNTGAKQTDASSGQDTVYGSNGNDHIVSTNGGGDHLLGYAGNDVLVGGDAIQGDTINGGT 5041
F T + A G S + G+ VGG +I G
Sbjct: 281 FYTATDSSKALIFSVWDAGGTDTFD-FSGYSNNQRINLNEGSFSDVGGLK-GNVSIAHGV 338

Query: 5042 GNDILVAGLGQDSLYGGEGNDI 5063
+ + G G D L G ++I
Sbjct: 339 TIENAIGGSGNDILVGNSADNI 360



Score = 33.8 bits (77), Expect = 0.023
Identities = 16/75 (21%), Positives = 22/75 (29%), Gaps = 1/75 (1%)

Query: 4991 ASSGQDTVYGSNGNDHIVSTNGGGDHLLGYAGNDVLVGGDAIQGDTINGGTGNDILVAGL 5050
+S + + G GND + G D L G AG D V G D
Sbjct: 354 GNSADNILQGGAGNDVLYG-GAGADTLYGGAGRDTFVYGSGQDSTVAAYDWIADFQKGID 412

Query: 5051 GQDSLYGGEGNDIAV 5065
D ++
Sbjct: 413 KIDLSAFRNEGQLSF 427



Score = 33.4 bits (76), Expect = 0.032
Identities = 15/72 (20%), Positives = 22/72 (30%), Gaps = 1/72 (1%)

Query: 4983 NTGAKQTDASSGQDTVYGSNGNDHIVSTNGGGDHLLGYAGNDVLVGGDAIQGDTINGGTG 5042
N+ +G D +YG G D + G D + +G D V D G
Sbjct: 355 NSADNILQGGAGNDVLYGGAGADTL-YGGAGRDTFVYGSGQDSTVAAYDWIADFQKGIDK 413

Query: 5043 NDILVAGLGQDS 5054
D+
Sbjct: 414 IDLSAFRNEGQL 425


5Shewana3_0415Shewana3_0423Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0415119-3.677522hypothetical protein
Shewana3_0416123-4.318100dephospho-CoA kinase
Shewana3_0417223-3.991601type 4 prepilin peptidase 1
Shewana3_0418123-3.812167type II secretion system protein
Shewana3_0419122-3.397923type IV-A pilus assembly ATPase PilB
Shewana3_0420122-3.326867O-antigen polymerase
Shewana3_0421-123-0.979889methylation site containing protein
Shewana3_04221240.557411nicotinate-nucleotide pyrophosphorylase
Shewana3_04232320.542950N-acetyl-anhydromuranmyl-L-alanine amidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0417PREPILNPTASE332e-117 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 332 bits (853), Expect = e-117
Identities = 165/304 (54%), Positives = 203/304 (66%), Gaps = 15/304 (4%)

Query: 6 ISLLSHSLAQSPWLFITLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHADV 65
++LL PWL+ +L F+F+ IGSFLNVVIHR P+M++REWQ E Y
Sbjct: 1 MALLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPD---- 56

Query: 66 VEQIGIEKLNKPIDTYPEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCAACNTA 125
YNL+VP S CP C I N+P++ WL LRG+C C
Sbjct: 57 -----------DEGVDEPPYNLMVPRSCCPHCNHPITALENIPLLSWLWLRGRCRGCQAP 105

Query: 126 ISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVLTFVLIALTGIDLDEMLLPDQMTLPL 185
IS+RYP++EL+T LL +A P W + A +LT+VL+ALT IDLD+MLLPDQ+TLPL
Sbjct: 106 ISARYPLVELLTALLSVAVAMTLAPGWGTLAALLLTWVLVALTFIDLDKMLLPDQLTLPL 165

Query: 186 LWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAVFGAW 245
LW GLL NL F + DAVIGA AGYL LWS++W FKLLTGKEGMGYGDFKLLA GAW
Sbjct: 166 LWGGLLFNLLGGFVSLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAW 225

Query: 246 LGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQPIVDWY 305
LGWQ LP+V+LLSSLVGA +GI LI+L+ + + PIPFGPY+A AGWIAL+WG I WY
Sbjct: 226 LGWQALPIVLLLSSLVGAFMGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWY 285

Query: 306 LSTL 309
L+
Sbjct: 286 LTNF 289


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0418BCTERIALGSPF391e-136 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 391 bits (1007), Expect = e-136
Identities = 122/405 (30%), Positives = 214/405 (52%), Gaps = 9/405 (2%)

Query: 25 TFEWKGLNRDGQKTSGELRGASAAEIRSQLKSQGVNP--------KTVRKQSAALFKLGD 76
+ ++ L+ G+K G SA + R L+ +G+ P + S L
Sbjct: 3 QYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLRRK 62

Query: 77 AKISPMDIAMITRQIATMLAAGVPLVTTIELLGRGHEKAKMRELLGSILSEIQSGIPLSD 136
++S D+A++TRQ+AT++AA +PL ++ + + EK + +L+ ++ S++ G L+D
Sbjct: 63 IRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLAD 122

Query: 137 SLRPHRRYFDDLYVDLVAAGEHSGSLDVVFDRIATYREKSEALKSKIKKAMFYPAAVVVV 196
+++ F+ LY +VAAGE SG LD V +R+A Y E+ + ++S+I++AM YP + VV
Sbjct: 123 AMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLTVV 182

Query: 197 AILVTTLLLLFVVPQFEEIFKGFGAELPAFTQLVLHISRGLQSSWYIFLGAIVAGVFLFV 256
AI V ++LL VVP+ E F LP T++++ +S +++ L A++AG F
Sbjct: 183 AIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMAF- 241

Query: 257 RAHRNSQMVRDRVDEAVLKIPAIGPILHKGAMARFARTLATTFAAGVPLIDGLESAAGAS 316
R + R +L +P IG I AR+ARTL+ A+ VPL+ + +
Sbjct: 242 RVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDVM 301

Query: 317 GNAVYRKAILKIRQEVMAGMQMNVAMRTTGLFPDMLIQMVMIGEESGSLDNMLNKVANIY 376
N R + V G+ ++ A+ T LFP M+ M+ GE SG LD+ML + A+
Sbjct: 302 SNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADNQ 361

Query: 377 EMQVDDAVDGLSSLIEPIMMVVIGTVVGGLIVAMYLPIFQMGKVV 421
+ + + L EP+++V + VV +++A+ PI Q+ ++
Sbjct: 362 DREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLM 406


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0421BCTERIALGSPG405e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 39.9 bits (93), Expect = 5e-07
Identities = 14/32 (43%), Positives = 24/32 (75%), Gaps = 1/32 (3%)

Query: 5 KMNKNAQGFTLIELMIVVAIIGILAAVALPAY 36
+K +GFTL+E+M+V+ IIG+LA++ +P
Sbjct: 3 ATDKQ-RGFTLLEIMVVIVIIGVLASLVVPNL 33


6Shewana3_0492Shewana3_0518Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_04921173.153213formate-dependent nitrite reductase complex
Shewana3_0493-1182.6281164Fe-4S ferredoxin
Shewana3_04940163.640754polysulfide reductase, NrfD
Shewana3_0496-1173.498854copper-binding protein
Shewana3_0497-2152.972105ABC transporter-like protein
Shewana3_0498-2163.190080copper ABC transporter permease
Shewana3_0499-2121.831368transcriptional regulator
Shewana3_0500-2121.840050peptidase M13
Shewana3_05010111.087122fatty acid cistrans isomerase
Shewana3_05020120.639365peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_05030110.258027flavoprotein oxygenase
Shewana3_05040120.254928hypothetical protein
Shewana3_05051143.829455FMN reductase
Shewana3_05061153.9239933-octaprenyl-4-hydroxybenzoate carboxy-lyase
Shewana3_05073203.934603hypothetical protein
Shewana3_05083193.845947major facilitator superfamily transporter
Shewana3_05091172.396309short-chain dehydrogenase/reductase SDR
Shewana3_05103171.813010biotin carboxyl carrier protein
Shewana3_05111182.1537113-dehydroquinate dehydratase
Shewana3_05121183.213291peptidyl-tRNA hydrolase domain-containing
Shewana3_0513-1204.686391hypothetical protein
Shewana3_05140204.724316hypothetical protein
Shewana3_05150204.562038hypothetical protein
Shewana3_0516-1184.158070outer membrane efflux family protein
Shewana3_0517-2174.086235RND family efflux transporter MFP subunit
Shewana3_0518-2183.584552CzcA family heavy metal efflux protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0502SUBTILISIN1096e-28 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 109 bits (275), Expect = 6e-28
Identities = 59/269 (21%), Positives = 87/269 (32%), Gaps = 88/269 (32%)

Query: 210 TDRGPEFIGADQMWQGTATQGGLPVKGEGMVVGIIDTGINTDHVAFADDEEYARLNPYKG 269
RG E I A +W T +G G+ V ++DTG + DH
Sbjct: 22 IPRGVEMIQAPAVWNQT--------RGRGVKVAVLDTGCDADHPDLKA------------ 61

Query: 270 QAIGDCGAFPELCNNKLVGLHSYPEITDVYAAPEFQTSSGAKKRIRPANAEDYAGHGSHT 329
+++G ++ + + P +DY GHG+H
Sbjct: 62 ---------------RIIGGRNFTDDDE----------------GDPEIFKDYNGHGTHV 90

Query: 330 ASTVAGNTLKDTPLQGFTGDKVSDGVDVPFTFPQTSGVAPRAHIIAYQVCWPGTSGDPYA 389
A T+A ++ GVAP A ++ +V SG
Sbjct: 91 AGTIAAT-----------ENENG-----------VVGVAPEADLLIIKVLNKQGSGQ--- 125

Query: 390 GCPESAILSAFEDAIADGVDAINFSIGGAENMPWGDPMELAFLSAREAGISVAAAAGNSG 449
I+ AI VD I+ S+GG E+ + A A + I V AAGN G
Sbjct: 126 ---YDWIIQGIYYAIEQKVDIISMSLGGPED---VPELHEAVKKAVASQILVMCAAGNEG 179

Query: 450 AYWTADH------SSPWVTTVGATTHDRK 472
V +VGA DR
Sbjct: 180 DGDDRTDELGYPGCYNEVISVGAINFDRH 208



Score = 72.6 bits (178), Expect = 2e-15
Identities = 32/131 (24%), Positives = 48/131 (36%), Gaps = 32/131 (24%)

Query: 627 NNLATFSSLGPSKTNNTLVPDLTAPGVDIYAANADDQPFTNNPSASDWTFMSGTSMAAPH 686
+ + FS+ DL APG DI + + SGTSMA PH
Sbjct: 207 RHASEFSNSNNE-------VDLVAPGEDILSTVPG----------GKYATFSGTSMATPH 249

Query: 687 VTGAMTLLTQL-----HPDWTPAEIQSALMLTAGPVVLNTGYELVEPYYNFMAGAGAINV 741
V GA+ L+ QL D T E+ + L+ P+ + E G G + +
Sbjct: 250 VAGALALIKQLANASFERDLTEPELYAQLIKRTIPLGNSPKME----------GNGLLYL 299

Query: 742 ARAADTGLVMD 752
+ + D
Sbjct: 300 TAVEELSRIFD 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0507IGASERPTASE345e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.3 bits (78), Expect = 5e-04
Identities = 21/80 (26%), Positives = 31/80 (38%), Gaps = 2/80 (2%)

Query: 145 PMAYDDTPVAVSPPVRVTTSMQYSPSEGRMVSNMPTNSATVISQTGVSTTRASTASAEQM 204
P + +T P + T+S P N NS + T T ++E
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVN-TGNSVVENPENTTPATTQPTVNSE-S 1215

Query: 205 ANVPRARAARSVSSLPSNAR 224
+N P+ R RSV S+P N
Sbjct: 1216 SNKPKNRHRRSVRSVPHNVE 1235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0508TCRTETA514e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 51.0 bits (122), Expect = 4e-09
Identities = 60/322 (18%), Positives = 106/322 (32%), Gaps = 22/322 (6%)

Query: 52 VAHVSYAISAYALGVVVGSPIIMVLGVRIKRRTLLIALAAMMAVANGLSALAPSLNWLVF 111
AH ++ YAL +P++ L R RR +L+ A AV + A AP L L
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYI 101

Query: 112 FRFLSGLPHGAYFGVAMLLAASLVPPEMKARAVSRVIIGLTLATIVGVPFATWMGQTVGW 171
R ++G+ GA VA A + + +AR + + G MG
Sbjct: 102 GRIVAGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSP 159

Query: 172 RSGIGIVAIIAAVTAVMLYFLAPNVAVPQNASPKKELQTLKNREVWLTLGIAAIGFGGIF 231
+ A + + + FL P + + N +
Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPE---SHKGERRPLRREALNPLASFRWARGMTVVAALM 216

Query: 232 CVYTYLAETLIQVTQV------------EPFKIPVMMAVFGI-GATLGTLVCGWAADK-S 277
V+ ++ + + QV + I + +A FGI + ++ G A +
Sbjct: 217 AVF-FIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLG 275

Query: 278 ALAAAFWSLVLSTLVLALYPSLTGSYWALMPI-VFFVGSGIGLATTVQARLMDVAPDGQA 336
A ++ L + W PI V GIG+ V + Q
Sbjct: 276 ERRALMLGMIADGTGYILL-AFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG 334

Query: 337 MTGALVQCAFNLANAIGPWVGS 358
+ +L + +GP + +
Sbjct: 335 QLQGSLAALTSLTSIVGPLLFT 356


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0509DHBDHDRGNASE547e-11 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 53.9 bits (129), Expect = 7e-11
Identities = 41/183 (22%), Positives = 80/183 (43%), Gaps = 5/183 (2%)

Query: 2 ILITGASSGLGAALAALYAKDNQALTLTGRDANRLHTVANALSPFSTQSISAISADLADE 61
ITGA+ G+G A+A A + + +L V ++L + + A AD+ D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHA-EAFPADVRDS 69

Query: 62 ASVEALFDGL---TDTPNTVIHCAGSGYFGTLETQGTSEIQALLNNNVTSTILLVRELVK 118
A+++ + + + +++ AG G + + E +A + N T R + K
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129

Query: 119 RYK-QQAVKVVIVMSTAALTAKAGESTYCAAKWAVRGFIESVRLELKQSPMKLIAVYPGG 177
+++ +V V S A + + Y ++K A F + + LEL + ++ V PG
Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189

Query: 178 MDT 180
+T
Sbjct: 190 TET 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0510RTXTOXIND310.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.002
Identities = 10/32 (31%), Positives = 14/32 (43%)

Query: 120 IEAKRDGIVGAIWVKDGDEVAFDQPLFTLIET 151
I+ + IV I VK+G+ V L L
Sbjct: 99 IKPIENSIVKEIIVKEGESVRKGDVLLKLTAL 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0516RTXTOXIND330.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.9 bits (75), Expect = 0.002
Identities = 16/154 (10%), Positives = 45/154 (29%), Gaps = 12/154 (7%)

Query: 76 EVQAQIARQQQAELAIAAADRAIYNPEL-GLNYQNADTDTYSLGLSQTLDWGDKRGVATR 134
+ + QA L + EL L + Y +S+ + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 135 LAQLEAQILLADIQLERSQMLAERLLALAEQAQSNKALTFAEQQLRFTQAQLNIAEQRFA 194
+ + Q ++ L++ + AE+ + E R +++L+
Sbjct: 195 FSTWQNQKYQKELNLDKKR---------AERLTVLARINRYENLSRVEKSRLDDFSSLLH 245

Query: 195 AGDLSDVELQLLKLELASNTADYALAEQAALVAD 228
++ + + + + L + +
Sbjct: 246 KQAIAKHAVLEQENKYVEAVNE--LRVYKSQLEQ 277


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0517RTXTOXIND561e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 56.0 bits (135), Expect = 1e-10
Identities = 31/138 (22%), Positives = 55/138 (39%), Gaps = 9/138 (6%)

Query: 156 EVAKAQAEYINAAAEWSRVRR---MSEGAVSVSRRMQAQVDAELKRAILEAIKMTDAQIR 212
V + + +Y+ A E + E + ++ V K IL+ ++ T I
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 213 TLE----STPEAIGSYQLLAPIDGRVQQ-DIAMLGQVFTAGTPLMQLT-DESHLWVEAQL 266
L E + + AP+ +VQQ + G V T LM + ++ L V A +
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 267 TPAQAANVNVGGPALVQV 284
+NVG A+++V
Sbjct: 373 QNKDIGFINVGQNAIIKV 390



Score = 42.9 bits (101), Expect = 2e-06
Identities = 26/149 (17%), Positives = 54/149 (36%), Gaps = 5/149 (3%)

Query: 100 SFSNLNLDTMATATLVVDRDRTATLAPQLDVRVQARHVVPGQEVKKGEPLLTLGG----A 155
+ + A L R+ + P + V+ V G+ V+KG+ LL L A
Sbjct: 76 VLGQVEIVATANGKLTHS-GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 156 EVAKAQAEYINAAAEWSRVRRMSEGAVSVSRRMQAQVDAELKRAILEAIKMTDAQIRTLE 215
+ K Q+ + A E +R + +S D + + E + + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 216 STPEAIGSYQLLAPIDGRVQQDIAMLGQV 244
+ YQ +D + + + +L ++
Sbjct: 195 FSTWQNQKYQKELNLDKKRAERLTVLARI 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0518ACRIFLAVINRP6540.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 654 bits (1690), Expect = 0.0
Identities = 224/1077 (20%), Positives = 438/1077 (40%), Gaps = 72/1077 (6%)

Query: 9 AIKNRLLVVLALLAVVAASVAMLPKLNLDAFPDVTNVQVTINTAAEGLAAEEVEKLISYP 68
I+ + + + ++ A + +L + +P + V+++ G A+ V+ ++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 69 VESAMYALPAVTEVRSLS-RTGLSIVTVVFAEGTDIYFARQQVFEQLQAAREMIPSGVGV 127
+E M + + + S S G +T+ F GTD A+ QV +LQ A ++P V
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 128 PEIGPNTSGLGQIYQYILRADPSSGINAAELRSLNDYLVKLIMMPVGGVTDVLSFGGEVR 187
I S + +D + G ++ VK + + GV DV FG +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSD-NPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ-Y 182

Query: 188 QYQVQVDPNKLRAYGLSMAQVSEALESNNRNAGGWFMDQGQEQLVVRGYGMLPAGEAGLA 247
++ +D + L Y L+ V L+ N + G L + +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLG-GTPALPGQQLNASIIAQTRFK 241

Query: 248 AIAQIPLTEVR----GTPVRVGDIAQVDFGSEIRVGAVTMTRRDEAGQAQDLGEVVAGVV 303
+ +R G+ VR+ D+A+V+ G E + G+ +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARIN-----GK-----PAAGLGI 291

Query: 304 LKRMGANTKATIDDIDARINLIEQALPKGVSFEVFYDQADLVDKAVTTVRDALLMAFVFI 363
GAN T I A++ ++ P+G+ YD V ++ V L A + +
Sbjct: 292 KLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLV 351

Query: 364 VVILALFLVNIRATLLVLLSIPVSIGLALMVMSYYGLSANLMSLGGLAVAIGMLVDGSVV 423
+++ LFL N+RATL+ +++PV + +++ +G S N +++ G+ +AIG+LVD ++V
Sbjct: 352 FLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIV 411

Query: 424 MVENIFKHLTQPDRRHLAQARSRADGEVDPYHGDEDGSAHAVEADNNMAVRIMLAAKEVC 483
+VEN+ R + ++ P + ++
Sbjct: 412 VVENV--------------ERVMMEDKLPPKEA------------------TEKSMSQIQ 439

Query: 484 SPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMISALLVALIAVPALAVYLFK- 542
+ ++ VF P+ G G +++ +++I+ AM ++LVALI PAL L K
Sbjct: 440 GALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKP 499

Query: 543 ---------RGVVLKESVVLAPLDSAYRKLLSATLARPKLVMTSALLMFAMSMVLLPRLG 593
G + + Y + L + L+ A +VL RL
Sbjct: 500 VSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLP 559

Query: 594 TEFVPELEEGTINLRVTLAPTASLGTSLDVAPKLEAMLLEFPEVEYALSRIGAPELGGDP 653
+ F+PE ++G + L A+ + V ++ L+ + S
Sbjct: 560 SSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVE-SVFTVNGFSFSG 618

Query: 654 EPVSNIEVYIGLKPIEEWQSASSRLE--LQRLMEEKLSVFPGLLLTFSQPIATRVDELLS 711
+ + ++ LKP EE + E + R E + G ++ F+ P + EL +
Sbjct: 619 QAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMP---AIVELGT 675

Query: 712 GVKAQLA-IKLFGPDLDVLSEKGQVLTDLVAKIPGAV-DVSLEQVSGEAQLVVRPDRAQL 769
I G D L++ L + A+ P ++ V + AQ + D+ +
Sbjct: 676 ATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKA 735

Query: 770 ARYGISVDQVMSLVSQGIGGASAGQVIDGNARYDINLRLAAQYRSSPDVIKDLLLSGSNG 829
G+S+ + +S +GG ID + ++ A++R P+ + L + +NG
Sbjct: 736 QALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANG 795

Query: 830 ATVRLGEVASVEVEMAPPNIRRDDVQRRVVVQANVA-GRDMGSVVKDIYALVPQADLPAG 888
V + P + R + + +Q A G G + + L + LPAG
Sbjct: 796 EMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASK--LPAG 853

Query: 889 YTVIVGGQYENQQRAQQKLMLVVPISIALIALLLYFSFGAVKQVLLIMANVPLALIGGIV 948
G ++ + + +V IS ++ L L + + + +M VPL ++G ++
Sbjct: 854 IGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLL 913

Query: 949 ALFVSGTYLSVPSSIGFITLFGVAVLNGVVLVDSINQ-RRQSGESLYDSVYEGTVGRLRP 1007
A + V +G +T G++ N +++V+ + G+ + ++ RLRP
Sbjct: 914 AATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRP 973

Query: 1008 VLMTALTSALGLIPILVSSGVGSEIQKPLAVVIIGGLFSSTALTLLVLPTLYRWLYR 1064
+LMT+L LG++P+ +S+G GS Q + + ++GG+ S+T L + +P + + R
Sbjct: 974 ILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR 1030



Score = 106 bits (266), Expect = 2e-25
Identities = 85/550 (15%), Positives = 186/550 (33%), Gaps = 68/550 (12%)

Query: 10 IKNRLLVVLALLAVVAASVAMLPKLNLDAFPDVTNVQVTIN-TAAEGLAAEEVEKLI--- 65
+ + +L +VA V + +L P+ G E +K++
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 66 -SYPVESAMYALPAVTEVRSLSRTGLS----IVTVVFAEGTDIYFARQQVFEQLQAAREM 120
Y +++ + +V V S +G + + V + + A+
Sbjct: 594 TDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKME 653

Query: 121 ---IPSGVGVPEIGPNTSGLGQIYQYILRADPSSGINAAELRSLNDYLVKLIMMPVGGVT 177
I G +P P LG + +G+ L + L+ + +
Sbjct: 654 LGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLV 713

Query: 178 DVLSFGGEVR-QYQVQVDPNKLRAYGLSMAQVSEALES--NNRNAGGWFMDQGQEQLVVR 234
V G E Q++++VD K +A G+S++ +++ + + + ++L V+
Sbjct: 714 SVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQ 773

Query: 235 GYGMLPAGEA-GLAAIAQIPLTEVRGTPVRVGDIAQVDFGSEIRVGAVTMTRRDEAGQAQ 293
A + ++ + G V + G+ + R + +
Sbjct: 774 ----ADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVY----GSPRLERYNGLPSME 825

Query: 294 DLGEVVAGVVLKRMGANTKATIDDIDARINLIEQALPKGVSFEVFYDQADLVDKAVTTVR 353
GE G D A + + LP G+ ++ + + +
Sbjct: 826 IQGEAAPGTSS-----------GDAMALMENLASKLPAGIGYD-WTGMSYQERLSGNQAP 873

Query: 354 DALLMAFVFIVVILALFLVNIRATLLVLLSIPVSIGLALMVMSYYGLSANLMSLGGLAVA 413
+ ++FV + + LA + + V+L +P+ I L+ + + ++ + GL
Sbjct: 874 ALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTT 933

Query: 414 IGMLVDGSVVMVENIFKHLTQPDRRHLAQARSRADGEVDPYHGDEDGSAHAVEADNNMAV 473
IG+ ++++VE A+ +G+ VEA
Sbjct: 934 IGLSAKNAILIVE-------------FAKDLMEKEGK------------GVVEA------ 962

Query: 474 RIMLAAKEVCSPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMISALLVALIAV 533
++A + PI + I+ PL G + + ++ M+SA L+A+ V
Sbjct: 963 -TLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFV 1021

Query: 534 PALAVYLFKR 543
P V + +
Sbjct: 1022 PVFFVVIRRC 1031


7Shewana3_0595Shewana3_0601Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_05952160.152871DNA mismatch repair protein
Shewana3_0596320-0.787206tRNA delta(2)-isopentenylpyrophosphate
Shewana3_0597429-1.405806RNA-binding protein Hfq
Shewana3_0598529-1.631796HSR1-like GTP-binding protein
Shewana3_0599533-2.251818HflK protein
Shewana3_0600535-2.727123HflC protein
Shewana3_0601333-2.064240ubiquinol-cytochrome c reductase, iron-sulfur
8Shewana3_0618Shewana3_0645Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0618-1163.107131Sel1 domain-containing protein
Shewana3_0619-1162.8525652'-5' RNA ligase
Shewana3_0620-1162.092151diguanylate cyclase/phosphodiesterase
Shewana3_0621-1120.908097hypothetical protein
Shewana3_0622013-0.095897ATP-dependent helicase HrpB
Shewana3_0623117-2.512003penicillin-binding protein 1B
Shewana3_0624327-6.143836PpiC-type peptidyl-prolyl cis-trans isomerase
Shewana3_0625324-5.617297hypothetical protein
Shewana3_0626118-2.910741hypothetical protein
Shewana3_06270171.786483hypothetical protein
Shewana3_06280213.371481hypothetical protein
Shewana3_0629-1213.857268hypothetical protein
Shewana3_0631-1173.430462hypothetical protein
Shewana3_0632-1183.862655methyl-accepting chemotaxis sensory transducer
Shewana3_06330163.879732molydopterin dinucleotide-binding region
Shewana3_06340133.015697polysulfide reductase, NrfD
Shewana3_06351162.9519854Fe-4S ferredoxin
Shewana3_06362162.587604ATPase domain-containing protein
Shewana3_06371192.750847two component LuxR family transcriptional
Shewana3_06382202.494852hypothetical protein
Shewana3_0639-2181.139737hypothetical protein
Shewana3_0640-213-1.924103hypothetical protein
Shewana3_0641-117-4.617000hypothetical protein
Shewana3_0642-118-6.320632sodium:dicarboxylate symporter
Shewana3_0643021-6.933943hypothetical protein
Shewana3_0644-213-4.277043hypothetical protein
Shewana3_0645-214-3.944520hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0632BACSURFANTGN290.036 Yersinia/Haemophilus virulence surface antigen sign...
		>BACSURFANTGN#Yersinia/Haemophilus virulence surface antigen

signature.
Length = 322

Score = 28.9 bits (64), Expect = 0.036
Identities = 17/98 (17%), Positives = 39/98 (39%), Gaps = 16/98 (16%)

Query: 248 ASDITQRIIKSHAIRDAANIAHATSQSTVEQANRGVQLLDATVNTSNAIAAQTHKTTESM 307
++ +T+ +I +H ++ ++H+ Q + AT+ + + + + S
Sbjct: 23 SATLTEGVIGAHRVKVETALSHSNLQKKLS----------ATIKHNQSGRSMLDRKLTSD 72

Query: 308 LKLNEQSQSIQAIVATISAIADQTNLLALNAAIEAARA 345
K N++S T S I + L+ + A R
Sbjct: 73 GKANQRSSF------TFSMIMYRMIHFVLSTRVPAVRE 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0636PF06580330.003 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.003
Identities = 32/195 (16%), Positives = 74/195 (37%), Gaps = 33/195 (16%)

Query: 468 LQSVLTLIQQEVSRADSIISRLRNLLKK--RPVSKQLLYLHQLVNDTAPLLAY-ELEQ-- 522
L ++ LI ++ ++A +++ L L++ R + + + L + + +Y +L
Sbjct: 179 LNNIRALILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTV---VDSYLQLASIQ 235

Query: 523 --HHIQLSTNVSGEAYQLPLDEVGMQQLLLNLLKNAADACVQRQQSEVDASKPYQPTIDI 580
+Q ++ + + + +Q L+ N +K+ A P I +
Sbjct: 236 FEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGI------------AQLPQGGKILL 283

Query: 581 DLRYQEHKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLE 640
+ L V + G+ ++ + +S G GL V R + +G ++
Sbjct: 284 KGTKDNGTVTLEVENTGSLALKN---------TKESTGTGLQNVRER-LQMLYGTEAQIK 333

Query: 641 -TALSGGCQAQVSLP 654
+ G A V +P
Sbjct: 334 LSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0637HTHFIS978e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 97.2 bits (242), Expect = 8e-26
Identities = 29/119 (24%), Positives = 52/119 (43%)

Query: 7 VYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPGLSGPQL 66
+ + DDD ++R L L G ++R +A + I + DV MP + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 67 HAQLVQHNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKA 125
++ + L V+ ++ A++A + GA D+ KP D +L + +AL K
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0638GPOSANCHOR521e-08 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 52.0 bits (124), Expect = 1e-08
Identities = 53/316 (16%), Positives = 102/316 (32%), Gaps = 10/316 (3%)

Query: 598 EYAASEQELRIRLSKAEEALQSAQELQAEAESQLVAINGELDKLSRELTFARTAYKNSRD 657
EL LS A+E L+ + +E S++ + L + L A
Sbjct: 82 ALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSA 141

Query: 658 DLRRLFDEKRSEQDKINKALAERKAFAQQRLTQLDGELKQLKHQHQLWLEEQKEQALEAR 717
++ L EK + KA K + + E ++ LE
Sbjct: 142 KIKTLEAEK---AALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 198

Query: 718 MEKQAYWQEVIGALDNQLGQIKATIDARRESAKAEQKACETWYKNELKSRGVDEDNILKL 777
+E + A L KA + AR+ + + + + E L
Sbjct: 199 LEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 258

Query: 778 KQQIRELETKISRAEQRRSEVLRFDDWY-----QHTWLIRKPKLQTQLSDVKR-AASEID 831
+ + ELE + A + + Q+Q+ + R +
Sbjct: 259 EARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL 318

Query: 832 QQLKAKTQEVKTRRQQLETERKASDAAQIEASENLTKLRAVMRKLAELKLPANNEEAQGS 891
+ ++++ Q+LE + K S+A++ +L R ++L E + E+ + S
Sbjct: 319 DASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL-EAEHQKLEEQNKIS 377

Query: 892 LGERLRQGEDLLLKRD 907
R DL R+
Sbjct: 378 EASRQSLRRDLDASRE 393



Score = 33.5 bits (76), Expect = 0.006
Identities = 50/346 (14%), Positives = 112/346 (32%), Gaps = 27/346 (7%)

Query: 360 WRADMENLSERHKLQTEKHQDIEAAYNARRSKIGEQLNRELEGLHAEQDKQREARDKQRE 419
+ + + K+ D+ A + N EL + ++ DK
Sbjct: 55 VQERADKFEIENNTLKLKNSDLSFNNKAL-----KDHNDELTEELSNAKEKLRKNDKSLS 109

Query: 420 VARADLDALEAQWRSQMDAGKASFSEQEYQFKLNAAELKLRVDGVTYTEEEKLSLAIFDE 479
+ + LEA + D KA + +A L + + ++
Sbjct: 110 EKASKIQELEA---RKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADL----EK 162

Query: 480 RIHRADEEQESCNAKVERLTGEERKLRAKRDQANEALRIASLRVNERQTALDELHHMLFP 539
+ A + +AK++ L E+ L A++ + +AL A + L
Sbjct: 163 ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAA 222

Query: 540 QSHTL--LEFLRKEAQGWEQSLGKVIAPELLHRTDLHPTVTGESDTVFGVHLDLKAIDVP 597
+ LE + A + + I + L +
Sbjct: 223 LAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKAL------------E 270

Query: 598 EYAASEQELRIRLSKAEEALQSAQELQAEAESQLVAINGELDKLSRELTFARTAYKNSRD 657
++ E + + +A+ E Q +N L R+L +R A K
Sbjct: 271 GAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEA 330

Query: 658 DLRRLFDEKRSEQDKINKALAERKAFAQQRLTQLDGELKQLKHQHQ 703
+ ++L ++ + + ++L +++ QL+ E ++L+ Q++
Sbjct: 331 EHQKLEEQNKI-SEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375


9Shewana3_0792Shewana3_0797Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0792-121-4.271863aromatic acid decarboxylase
Shewana3_0793-122-4.703206hypoxanthine phosphoribosyltransferase
Shewana3_0794022-4.416253ABC transporter-like protein
Shewana3_0795-123-4.265576ABC transporter
Shewana3_0796-124-4.405550hypothetical protein
Shewana3_0797024-4.456959protease domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0795ABC2TRNSPORT711e-16 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 70.7 bits (173), Expect = 1e-16
Identities = 46/215 (21%), Positives = 97/215 (45%), Gaps = 1/215 (0%)

Query: 37 LYFLIFGNLVGSRIGDMGGVSYMEFIAPGLIMMSVITNS-YSNVASSFYSAKFQRNLEEL 95
+Y G +G +G +GGVSY F+A G++ S +T + + + ++F + QR E +
Sbjct: 44 IYLFGLGAGLGVMVGRVGGVSYTAFLAAGMVATSAMTAATFETIYAAFGRMEGQRTWEAM 103

Query: 96 MVAPVPHYVMIAGYVGGGVARGLCVGLIVTLVAMFFVDISLHHAGLVVMTVFLTSVLFSL 155
+ + ++ G + + G + +VA + + LT + F+
Sbjct: 104 LYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWLSLLYALPVIALTGLAFAS 163

Query: 156 GGLINAVFAKSFDDISIIPTFVLTPLTYLGGVFYSLSLLPSFWQGVSALNPVVYMINVFR 215
G++ A S+D T V+TP+ +L G + + LP +Q + P+ + I++ R
Sbjct: 164 LGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIR 223

Query: 216 YGFLGFADISIPLSIGIMVGFCAVLWGVAYYLISR 250
LG + + +G + + + + ++ L+ R
Sbjct: 224 PIMLGHPVVDVCQHVGALCIYIVIPFFLSTALLRR 258


10Shewana3_0810Shewana3_0823Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_08100163.378569DEAD/DEAH box helicase
Shewana3_08110193.146164hypothetical protein
Shewana3_08121173.074190cysteine/glutathione ABC transporter
Shewana3_0813-1151.830629cysteine/glutathione ABC transporter
Shewana3_08140161.216507putative adenylyl cyclase CyaB
Shewana3_08151181.538974IS4 family transposase
Shewana3_08162270.158239DNA binding domain-containing protein
Shewana3_0817228-0.199774hypothetical protein
Shewana3_0818123-0.031887hypothetical protein
Shewana3_08190200.664732bifunctional proline
Shewana3_0820-115-0.355214SH3 type 3 domain-containing protein
Shewana3_0821-113-0.377783phosphate transporter
Shewana3_0822312-0.411806hypothetical protein
Shewana3_0823213-0.185167adenylate cyclase
11Shewana3_0873Shewana3_0878Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0873116-5.627351putative lipoprotein
Shewana3_0874320-6.616376DSBA oxidoreductase
Shewana3_0875219-6.251904formate dehydrogenase
Shewana3_0876118-6.166936dihydropteridine reductase
Shewana3_0877117-6.071428sugar-binding protein
Shewana3_0878115-5.520519hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0876ALARACEMASE270.047 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 27.4 bits (61), Expect = 0.047
Identities = 9/38 (23%), Positives = 18/38 (47%)

Query: 165 EGFDAAKLNQVLGLREKGLCASVVVALGYRSEEDFNAK 202
+GF L + + LRE+G +++ G+ +D
Sbjct: 54 DGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIY 91


12Shewana3_0930Shewana3_0942Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_09302141.722916O-acetylhomoserine/O-acetylserine sulfhydrylase
Shewana3_09312151.331271hypothetical protein
Shewana3_09320160.231975methyltransferase small
Shewana3_0933-115-0.422020DNA-N1-methyladenine dioxygenase
Shewana3_0934-116-0.129942transcriptional regulator BolA
Shewana3_0935-116-0.461914TRAP dicarboxylate transporter- DctP subunit
Shewana3_0936122-1.358998S-ribosylhomocysteinase
Shewana3_0937224-1.453818TonB-dependent receptor
Shewana3_0938340-1.868032Na(+)-translocating NADH-quinone reductase
Shewana3_0939233-2.042418Na(+)-translocating NADH-quinone reductase
Shewana3_0940229-2.841777Na(+)-translocating NADH-quinone reductase
Shewana3_0941324-2.893817Na(+)-translocating NADH-quinone reductase
Shewana3_0942320-2.743275Na(+)-translocating NADH-quinone reductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0936LUXSPROTEIN2724e-97 Bacterial autoinducer-2 (AI-2) production protein Lu...
		>LUXSPROTEIN#Bacterial autoinducer-2 (AI-2) production protein LuxS

signature.
Length = 171

Score = 272 bits (696), Expect = 4e-97
Identities = 131/168 (77%), Positives = 150/168 (89%)

Query: 2 PLLDSFTVDHTRMNAPAVRVAKHMSTPKGDAITVFDLRFCAPNKDILSERGIHTLEHLFA 61
PLLDSFTVDHTRMNAPAVRVAK M TPKGD ITVFDLRF APNKDILSE+GIHTLEHL+A
Sbjct: 1 PLLDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTAPNKDILSEKGIHTLEHLYA 60

Query: 62 GFMRDHLNGSDVEIIDISPMGCRTGFYMSLIGEPSERQVADAWLASMEDVLKVVEQSEIP 121
GFMR+HLNG VEIIDISPMGCRTGFYMSLIG PSE+QVADAW+A+MEDVLKV Q++IP
Sbjct: 61 GFMRNHLNGDSVEIIDISPMGCRTGFYMSLIGTPSEQQVADAWIAAMEDVLKVENQNKIP 120

Query: 122 ELNEYQCGTYEMHSLEQAQDIARNIIAAGVSVNRNDDLKLSDEILGQL 169
ELNEYQCGT MHSL++A+ IA+NI+ GV+VN+ND+L L + +L +L
Sbjct: 121 ELNEYQCGTAAMHSLDEAKQIAKNILEVGVAVNKNDELALPESMLREL 168


13Shewana3_1162Shewana3_1176Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_11623190.184302DEAD/DEAH box helicase
Shewana3_11632190.193438hypothetical protein
Shewana3_11642190.087478hypothetical protein
Shewana3_11652200.451261MerR family transcriptional regulator
Shewana3_1166-1180.349575deoxyribodipyrimidine photo-lyase type I
Shewana3_1167-218-0.514507transcriptional regulator-like protein
Shewana3_1168-218-0.212621short-chain dehydrogenase/reductase SDR
Shewana3_1169-118-0.690880amine oxidase
Shewana3_1170-118-0.988941hypothetical protein
Shewana3_1171-117-1.459946cyclopropane-fatty-acyl-phospholipid synthase
Shewana3_1172021-2.932456hypothetical protein
Shewana3_1173024-3.829480hypothetical protein
Shewana3_1174-124-4.091145hypothetical protein
Shewana3_1175026-5.500610hypothetical protein
Shewana3_1176021-3.478747hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1168DHBDHDRGNASE502e-09 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 50.0 bits (119), Expect = 2e-09
Identities = 40/189 (21%), Positives = 75/189 (39%), Gaps = 10/189 (5%)

Query: 12 VLITGASSGIGLQLAKDYLAAGWHVIACGRDKAKLDALAETVLIGA---TCISFDINERS 68
ITGA+ GIG +A+ + G H+ A + KL+ + ++ A D+ + +
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70

Query: 69 QVQENALRIKDLLTQCACQLDLVILNAGGCEYIDDAKHFDDRLFERVVHTNLIAMGFCLG 128
+ E RI+ + +D+++ N G D +E N +
Sbjct: 71 AIDEITARIEREMGP----IDILV-NVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 129 AFLPLMP--RGARLALMSSSATYLAFPRAEAYGASKAGVQYLAASLRLDLAQHGISVSVI 186
+ M R + + S+ + AY +SKA L L+LA++ I +++
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 187 CPGFVATPL 195
PG T +
Sbjct: 186 SPGSTETDM 194


14Shewana3_1240Shewana3_1335Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_12402271.914849phosphoribosylformylglycinamidine synthase
Shewana3_12413261.717361cytochrome bd ubiquinol oxidase subunit I
Shewana3_12423254.241784cytochrome d ubiquinol oxidase subunit II
Shewana3_12433306.679033cyd operon protein YbgT
Shewana3_12443296.886537phage integrase family protein
Shewana3_12455307.119282hypothetical protein
Shewana3_12467327.813095transcriptional regulator-like protein
Shewana3_12478337.701924hypothetical protein
Shewana3_12488347.213252hypothetical protein
Shewana3_12497307.305712ATPase-like protein
Shewana3_12507296.201032integrase catalytic subunit
Shewana3_12516284.919025IstB ATP binding domain-containing protein
Shewana3_12526294.129020DNA repair protein RadC
Shewana3_12535283.998806hypothetical protein
Shewana3_12545294.623796DNA helicase-like protein
Shewana3_12556314.850844hypothetical protein
Shewana3_12564327.389262hypothetical protein
Shewana3_125753510.604591patatin
Shewana3_125873913.712396XRE family transcriptional regulator
Shewana3_125984114.582144putative lipoprotein
Shewana3_126173914.613769hypothetical protein
Shewana3_126284113.784350hypothetical protein
Shewana3_126393713.199941putative replication initiator and transcription
Shewana3_126483312.452478cobyrinic acid a,c-diamide synthase
Shewana3_126553210.980674hypothetical protein
Shewana3_126653110.690119hypothetical protein
Shewana3_126743110.195395TraF peptidase
Shewana3_12684319.954380hypothetical protein
Shewana3_12692307.566872XRE family transcriptional regulator
Shewana3_12702327.638356major facilitator superfamily transporter
Shewana3_12715318.527168LysR family transcriptional regulator
Shewana3_12723339.511188hypothetical protein
Shewana3_12735349.822448Smp-30/Cgr1 family protein
Shewana3_127453410.036342Smp-30/Cgr1 family protein
Shewana3_127553511.388823IclR family transcriptional regulator
Shewana3_127653812.122008hypothetical protein
Shewana3_127764013.446964major facilitator superfamily transporter
Shewana3_127884614.219662LysR family transcriptional regulator
Shewana3_127985015.242937putative lipoprotein
Shewana3_128075616.139709conjugal transfer coupling protein TraG
Shewana3_128196317.462147CopG/DNA-binding domain-containing protein
Shewana3_128286317.650347type II secretion system protein E
Shewana3_128386417.482913conjugal transfer protein TrbC
Shewana3_128486317.503847putative conjugal transfer trbD transmembrane
Shewana3_128576217.509450conjugal transfer ATPase TrbE
Shewana3_128686217.886804conjugal transfer protein TrbJ
Shewana3_128776018.082757putative lipoprotein
Shewana3_128834412.700136conjugal transfer protein TrbL
Shewana3_128923710.376863conjugal transfer protein TrbF
Shewana3_12900267.097679conjugal transfer protein TrbG/VirB9/CagX
Shewana3_1291-1215.299307conjugation TrbI family protein
Shewana3_1292-2182.554946hypothetical protein
Shewana3_1293-3171.960331methyl-accepting chemotaxis sensory transducer
Shewana3_1294-2182.081469hypothetical protein
Shewana3_1295-2172.099199acriflavin resistance protein
Shewana3_1296-220-4.721005RND family efflux transporter MFP subunit
Shewana3_1297-124-6.581283TetR family transcriptional regulator
Shewana3_1298-122-5.838994hypothetical protein
Shewana3_1299122-6.292929hypothetical protein
Shewana3_1300125-6.449654hypothetical protein
Shewana3_1301226-6.634445hypothetical protein
Shewana3_1302323-4.119755polysaccharide biosynthesis protein
Shewana3_1303224-3.858608polysaccharide biosynthesis protein
Shewana3_1304326-4.609376N-acetylneuraminate synthase
Shewana3_1305526-5.021815hypothetical protein
Shewana3_1306424-4.453836hypothetical protein
Shewana3_1307424-4.394740hypothetical protein
Shewana3_1308323-3.247347N-acetyltransferase GCN5
Shewana3_1309320-2.118714acylneuraminate cytidylyltransferase
Shewana3_1310220-2.206426hypothetical protein
Shewana3_1311019-2.349131hypothetical protein
Shewana3_1312019-2.657488hypothetical protein
Shewana3_1313-118-2.736081*hypothetical protein
Shewana3_1314-118-3.484015hypothetical protein
Shewana3_1315018-4.068148hypothetical protein
Shewana3_1316118-4.308854FlgN family protein
Shewana3_1317018-4.197404anti-sigma-28 factor FlgM
Shewana3_1318-120-4.626337flagellar basal body P-ring biosynthesis protein
Shewana3_1319022-3.990028response regulator receiver modulated CheW
Shewana3_1320023-3.113480chemotaxis protein CheR
Shewana3_1321223-2.880900flagellar basal body rod protein FlgB
Shewana3_1322223-2.856214flagellar basal body rod protein FlgC
Shewana3_1323224-2.952221flagellar basal body rod modification protein
Shewana3_1324123-2.472489flagellar hook protein FlgE
Shewana3_1325-125-2.989144flagellar basal body rod protein FlgF
Shewana3_1326-126-3.846569flagellar basal body rod protein FlgG
Shewana3_1327-129-4.367629flagellar basal body L-ring protein
Shewana3_1328131-4.818899flagellar basal body P-ring protein
Shewana3_1329131-4.988498flagellar rod assembly protein/muramidase FlgJ
Shewana3_1330236-6.361759flagellar hook-associated protein FlgK
Shewana3_1331637-7.146169flagellar hook-associated protein FlgL
Shewana3_1332640-7.849937flagellin domain-containing protein
Shewana3_1333435-6.618565flagellin domain-containing protein
Shewana3_1334330-5.198591flagellar protein FlaG protein
Shewana3_1335125-4.259888flagellar hook-associated 2 domain-containing
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1240OMS28PORIN310.019 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 31.3 bits (70), Expect = 0.019
Identities = 32/138 (23%), Positives = 61/138 (44%), Gaps = 11/138 (7%)

Query: 138 VLDDFAKADVLFKRTEPAPFKSVNVLAEGRRAL------EVANVEMGLALAEDEIDYLVE 191
++ D AK V+ + K ++AEG + V + +++A E +L+E
Sbjct: 102 LMSDVAKGTVVASQEATIVAKCSGMVAEGANKVVEMSKKAVQETQKAVSVA-GEATFLIE 160

Query: 192 NFVRLNRNPNDIELMMFAQ--ANSEHCRHKIFNADWTIDGEAQ-PKSLFKMIKNTFETTP 248
+ LN++PN+ EL + + A E + + ++ +D Q + + M+ +
Sbjct: 161 KQIMLNKSPNNKELELTKEEFAKVEQVKETLMASERALDETVQEAQKVLNMVNGLNPSNK 220

Query: 249 DHVLSAYKDNAAVMEGSV 266
D VL A KD A + V
Sbjct: 221 DQVL-AKKDVAKAISNVV 237


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1251HTHFIS353e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.8 bits (80), Expect = 3e-04
Identities = 19/106 (17%), Positives = 39/106 (36%), Gaps = 9/106 (8%)

Query: 18 ADAVQQQLEQASTYEGLPFIERLSLLVEHEQLSREQRKQARLVKQARLKLQATVQEVDYQ 77
A++ + A Y PF + + L+ +R+ ++L ++ + + Q
Sbjct: 88 MTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQ 147

Query: 78 SARNLERSQVANLAQGEWLRRGQNLLITGPCGCGKTYLACALGYQA 123
+ ++ + L+ITG G GK +A AL
Sbjct: 148 EIYRVLA-RLM--------QTDLTLMITGESGTGKELVARALHDYG 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1254GPOSANCHOR350.001 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 35.4 bits (81), Expect = 0.001
Identities = 40/281 (14%), Positives = 83/281 (29%), Gaps = 19/281 (6%)

Query: 437 FYGQRPFGSDDKADPDAEDEAELDDEFGDAVNTLFSTPSTVEEKFGASEESDEAPADKEE 496
D D E+ + ++ +L S ++E + ++A
Sbjct: 75 DLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMN 134

Query: 497 GPKGFLGWLSACAEVNKTRSPEQRQALWQEAIADYEAAKTEVRRTC----AAANRIRELI 552
+ ++ L ++A+ T A +
Sbjct: 135 FSTADSAKIKTLEAEKA-ALAARKADL-EKALEGAMNFSTADSAKIKTLEAEKAALEARQ 192

Query: 553 QALCKTRKQVAEQSEALRTLESKLADAVNQLSRL--DAEENRPASMALKQCLY------- 603
L K + S A L L+ D E+ +M
Sbjct: 193 AELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLE 252

Query: 604 -ELEVHQARKPGFWAKVFSFWGAQRDWDAKRKRLESHHDLAKSEFSRIARLTKQLGASRE 662
E +AR+ + AK K LE+ ++E + + ++ L A+R+
Sbjct: 253 AEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQ 312

Query: 663 SLEKHTADTRRALQRLRSQCQ--AHMQDAIDVAREG-QADH 700
SL + +R A ++L ++ Q + +R+ + D
Sbjct: 313 SLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDL 353


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1263FLGMRINGFLIF280.048 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 28.0 bits (62), Expect = 0.048
Identities = 16/44 (36%), Positives = 25/44 (56%), Gaps = 6/44 (13%)

Query: 225 QFDVPYLYRK-SGSLAQPRDFARDLRALVAKQSLP-----GYEL 262
Q ++PY + SG++ P D +LR +A+Q LP G+EL
Sbjct: 71 QMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFEL 114


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1270TCRTETB1082e-27 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 108 bits (270), Expect = 2e-27
Identities = 93/461 (20%), Positives = 189/461 (40%), Gaps = 28/461 (6%)

Query: 36 LLLAGFVTIFDLFVVNVAIPSMQANLGASFAQIGFIVAGYELAFGVLLIMGGRLGDLFGR 95
L + F ++ + V+NV++P + + A ++ + L F + + G+L D G
Sbjct: 19 LCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGI 78

Query: 96 RRLFIVGMAGFTLASAMCGLAPS-AEILIVARVLQGLAAALLFPQVYASIRVNFDGDDSR 154
+RL + G+ S + + S +LI+AR +QG AA FP + + + ++R
Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAA-AFPALVMVVVARYIPKENR 137

Query: 155 -RAFGLLGMTLGLAAIAGQVLGGWLVHADLFGLSWRTIFLINVPIGLFAITAASCIPESR 213
+AFGL+G + + G +GG + H + W + LI + I + + + +
Sbjct: 138 GKAFGLIGSIVAMGEGVGPAIGGMIAHY----IHWSYLLLIPM-ITIITVPFLMKLLKKE 192

Query: 214 AEQSPALDWSGVILVSSGLALLLVPLIEGPGQGWPAWSLWSLGGAVALMVTFYRYQERQR 273
D G+IL+S G+ ++ + +S+ + +++F + + R
Sbjct: 193 VRIKGHFDIKGIILMSVGIVFFMLFT-----------TSYSISFLIVSVLSFLIFVKHIR 241

Query: 274 IAGRFPLVDMQLMRQRRFALGALLVLLVYSTSSSFFLSFALLVQTGLGLDPFVAGSIFA- 332
P VD L + F +G L +++ T + F +++ L GS+
Sbjct: 242 KVTD-PFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIF 300

Query: 333 PCSVGFVLASLTAPRLVALWGARAIVAGALVYAISIGLLITQVQMAGAELLPVTLIPVLI 392
P ++ ++ LV G ++ + + +S+ L + +I +
Sbjct: 301 PGTMSVIIFGYIGGILVDRRGPLYVLNIGVTF-LSVSFLTASFLLETTSWFMTIII---V 356

Query: 393 IVGAGQGLIMTPLLNLVLGFVKENQAGMASGVISTVQQVGAALGVAIVGILFGTALTASN 452
V G T + +V +K+ +AG +++ + G+AIVG L L
Sbjct: 357 FVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQR 416

Query: 453 GALAQADQYASAFVSGMLYNLGAALLICFL--LLILARSQR 491
+ D ++ S +L ++I +L L + SQR
Sbjct: 417 LLPMEVD-QSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQR 456


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1276PF00577300.002 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 30.2 bits (68), Expect = 0.002
Identities = 14/69 (20%), Positives = 25/69 (36%), Gaps = 4/69 (5%)

Query: 70 SLELGAAAQPGGAIYTLEATNGGLISFGGGVVLHDELGRTIGAV---GVAGATVEADQII 126
+ +G + + GG+++ GV L L T+ V G A VE +
Sbjct: 679 NANIGYSHSDDIKQLYYGVS-GGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGV 737

Query: 127 ALQAAGRSL 135
G ++
Sbjct: 738 RTDWRGYAV 746


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1277TCRTETA583e-11 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 57.5 bits (139), Expect = 3e-11
Identities = 97/384 (25%), Positives = 145/384 (37%), Gaps = 27/384 (7%)

Query: 16 VTAFAIGVAEFIVVGILPAIADDL---NVPLARAGGLVGLYALALAVGTPIVVLTLARLP 72
T V +++ +LP + DL N A G L+ LYAL P++ R
Sbjct: 12 STVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFG 71

Query: 73 RKPLLMALVAVFLAGNLISALSASYELLLAGRILTAVAHGSFFAIGATVAARLAPKGQAS 132
R+P+L+ +A I A + +L GRI+ + G+ A+ A + + +
Sbjct: 72 RRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDERA 130

Query: 133 RAIAMMFAGLTLAMVVGVPLGSLIGNALGWRLPFFAVAGLAGLAFLATARWVP------- 185
R M A MV G LG L+G PFFA A L GL FL +P
Sbjct: 131 RHFGFMSACFGFGMVAGPVLGGLMG-GFSPHAPFFAAAALNGLNFLTGCFLLPESHKGER 189

Query: 186 -ALPTQATGPVGTQLAALASAPILAMMAITVLG--FGASFATFTFINPILTDITGFSTHT 242
L +A P+ + A + A+MA+ + G A I D + T
Sbjct: 190 RPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGE--DRFHWDATT 247

Query: 243 VSLLLVVFGVA-TLVGNLAGGRWAASLGWPVALRRMLAGLLLVLVALAVALPLKWVMVPL 301
+ + L FG+ +L + G AA LG AL + + LA A W+ P+
Sbjct: 248 IGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRG-WMAFPI 306

Query: 302 LFVWGVLAFGMSPGFQAGMLETAGRWTPRAVDFASALNISAFNLGITLGETLGSGLVAQE 361
+ + GM P QA + + +L +G L + + A
Sbjct: 307 MVLLASGGIGM-PALQAMLSRQVDE---ERQGQLQGSLAALTSLTSIVGPLLFTAIYAAS 362

Query: 362 QMDMTPWA---GVALVLLAQLPLL 382
WA G AL LL LP L
Sbjct: 363 ITTWNGWAWIAGAALYLLC-LPAL 385


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1288PRTACTNFAMLY300.020 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 30.4 bits (68), Expect = 0.020
Identities = 35/154 (22%), Positives = 53/154 (34%), Gaps = 23/154 (14%)

Query: 254 GIFGPGIATGLVSGAPQL----GAGAMAGAAVGAVGTGVAIGAAATGVGAAVAAGARMAP 309
G FGPG ++ G + + +A + V A G AI G V+ G+ AP
Sbjct: 281 GGFGPGGFGPVLDGWYGVDVSGSSVELAQSIVEAPELGAAI-RVGRGARVTVSGGSLSAP 339

Query: 310 AAAKLAGAGARAATSAAGNARSAFQAGSTAAGGG-------------AKGAAAGLGNVAK 356
+ GAR A QAG+ A G G A G++
Sbjct: 340 HGNVIETGGARRFAPQAAPLSITLQAGAHAQGKALLYRVLPEPVKLTLTGGADAQGDIVA 399

Query: 357 TSAQAASRRVASGASAAGQKMTSSFRAGWNGSSD 390
T + G S + + +A W G++
Sbjct: 400 TELPS-----IPGTSIGPLDVALASQARWTGATR 428


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1290PF03544280.043 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 28.0 bits (62), Expect = 0.043
Identities = 13/73 (17%), Positives = 21/73 (28%)

Query: 26 PPPTISLDESVLAQPLPEPLAPVEVVAVPEPLALPAQLKPLPEVDAAPAAPEPADEKVRV 85
PP + + +P PEP E + + KP P+ +P + V
Sbjct: 62 PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPV 121

Query: 86 SRANAEARIAPTR 98
A
Sbjct: 122 ESRPASPFENTAP 134


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1293IGASERPTASE340.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.3 bits (78), Expect = 0.002
Identities = 35/264 (13%), Positives = 85/264 (32%), Gaps = 35/264 (13%)

Query: 393 QASVQSIEQQASKAQRIAKQNGEEAQALMQQTDQIATAIEEMSTSIRDVANHAQDGANQS 452
+V+ EQ A++ ++ +EA++ ++ Q AQ G+
Sbjct: 1048 SKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEV--------------AQSGSETK 1093

Query: 453 QQVDLAAKEGQQQQTQVVQDLLKLSQQLSSSHQAVEKVSQE-SEAISKVTEVINSIAEQT 511
+ KE + + + Q + QE SE + E +
Sbjct: 1094 ETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAE-----PARE 1148

Query: 512 NLLALNAAIEAARAGEQGRGFAVVADEVRTLAQRTQSSI---LEISQTIDKLQSQVKTTT 568
N +N ++ + A+ T S++ + S T++ S V+
Sbjct: 1149 NDPTVNIKEPQSQTNTTA--------DTEQPAKETSSNVEQPVTESTTVNTGNSVVENPE 1200

Query: 569 SQMAQSHQLGIASANQGEETGKQLEEITRRIGELAISSRNIASATEQQSSVAQEITHNLH 628
+ + Q + S + + + + + +++ +S+VA + +
Sbjct: 1201 NTTPATTQPTVNSESSNKPKNRHRRSVRSV----PHNVEPATTSSNDRSTVALCDLTSTN 1256

Query: 629 QISELANEGEHRAAETVNSANDLS 652
+ L++ +N +S
Sbjct: 1257 TNAVLSDARAKAQFVALNVGKAVS 1280


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1295ACRIFLAVINRP380e-117 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 380 bits (977), Expect = e-117
Identities = 208/1046 (19%), Positives = 431/1046 (41%), Gaps = 52/1046 (4%)

Query: 1 MIKAFVENGRLVSLVIALLLVAGFGAMSSLPRTEDPHITNRFASVITPYPGASAERVEAL 60
M F+ ++ +L++AG A+ LP + P I SV YPGA A+ V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTEVLENQLRRLEEIKLIQSTS-RPGISVIQLELKDTVTDTDPVWSR--ARDLLADARNT 117
VT+V+E + ++ + + STS G I L + TDP ++ ++ L A
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQS---GTDPDIAQVQVQNKLQLATPL 117

Query: 118 LPDGIQTPTL-DDQVGYAYTAILSLVWNDSSQPRVDMLNRYAKELQSRLRLLSGTDFVKL 176
LP +Q + ++ +Y + V ++ + D+ + A ++ L L+G V+L
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 177 YGAPEEEILVQLDGYKMSQLQLTPGTIAKILSSADSKIAAGEINN------NHFRALVEV 230
+GA + + + LD +++ +LTP + L + +IAAG++ A +
Sbjct: 178 FGA-QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 231 SGELDSQSRIRQVPLKVDAQGQIIRLGDIAHISRQPKTPADSIALVDGEQGVFVAARMLN 290
+ +V L+V++ G ++RL D+A + + + IA ++G+ + ++
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENY-NVIARINGKPAAGLGIKLAT 295

Query: 291 NTRVDIWQGQVKQLVDEFNQELPANIKVQWLFEQNSYTSERLGGLIVNLLQGFVIILAVL 350
+K + E P +KV + ++ + + ++ L + +++ V+
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVM 355

Query: 351 LLTLG-LRNAIIVALSLPLTALFTLACMKYIGLPIHQMSVTGLVVALGIMVDNAIVIVDA 409
L L +R +I +++P+ L T A + G I+ +++ G+V+A+G++VD+AIV+V+
Sbjct: 356 YLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVEN 415

Query: 410 IAQRRQ-QGMSRLSAVSETLHHLWLPLAGSTITTILAFAPIVLMPGAAGEFVGGIAMSVM 468
+ + + A +++ + L G + F P+ G+ G +++++
Sbjct: 416 VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIV 475

Query: 469 FALLGSYVISHTLIAGLAGRF--SLEGKHP-------VWYQHGINVPLVSGYFQASLRFA 519
A+ S +++ L L + +H W+ + V+ Y S+
Sbjct: 476 SAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFD-HSVNHY-TNSVGKI 533

Query: 520 LNRPLLSATFIGIIPLLGFYASGKMTEQFFPPSDRDMFQIELYLAPHVSLENTLNQV-QL 578
L +I ++ F P D+ +F + L + E T + Q+
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 579 MDKQLHQIEGIIQVDWVVGGNTPSFYYNLTQRQQGATNYAQAMVK-----ASDFERANAL 633
D L + ++ + V G ++ + + Q A A +K D A A+
Sbjct: 594 TDYYLKNEKANVESVFTVNG------FSFSGQAQNA-GMAFVSLKPWEERNGDENSAEAV 646

Query: 634 IPELQQTLDK---AFPEAQVLVRKLEQGPPFNAPVELM-IFGPNLETLRSLGDEVRNILA 689
I + L K F + +E G EL+ G + L +++ + A
Sbjct: 647 IHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAA 706

Query: 690 ATP-DVLHTRATLSAGAPKVWLQVNEDASLISGLTLTDIARQVQMATTGVIGGSVLEQTE 748
P ++ R + L+V+++ + G++L+DI + + A G +++
Sbjct: 707 QHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGR 766

Query: 749 SLPIRVRLGDTSREQASRLSEIQLVTPSGTAVPLSALSHNEVQVSRGAIPRRNGQRVNTI 808
+ V+ R + ++ + + +G VP SA + + + R NG I
Sbjct: 767 VKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEI 826

Query: 809 EAYIVSGVLPAQVLNDVKAKVAAISLPAGYRIEIGGESAKRNEAVGNLLSNLILVVTLLL 868
+ G + ++ A LPAG + G S + + + + + ++
Sbjct: 827 QGEAAPGTSSGDAMALMEN--LASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVF 884

Query: 869 ATVVLSFNSFRLTAIILLSALQSAGLGLLAVYVFGYPFGFPVIIALLGLMGLAINAAIVI 928
+ + S+ + ++L LLA +F ++ LL +GL+ AI+I
Sbjct: 885 LCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILI 944

Query: 929 LAELEDTDNARA-GDKEVIITTVSSCGRHISSTTITTVGGFIPLII---AGGGFWPPFAI 984
+ +D G E + V R I T++ + G +PL I AG G I
Sbjct: 945 VEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGI 1004

Query: 985 AIAGGTLLTTLLSLVWVPTMYLLLMK 1010
+ GG + TLL++ +VP ++++ +
Sbjct: 1005 GVMGGMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1296RTXTOXIND415e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.4 bits (97), Expect = 5e-06
Identities = 30/117 (25%), Positives = 51/117 (43%), Gaps = 4/117 (3%)

Query: 75 SGKLSELTVDSGAKVTQGQVLAKLDTRLLDAEHQEIQASLAQTQADVDLATSTLNRNLEL 134
+ + E+ V G V +G VL KL +A+ + Q+SL Q + + L+R++EL
Sbjct: 104 NSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ-TRYQILSRSIEL 162

Query: 135 KKSGYVSEQLLDENRTQLASLE-AAKKRLLASQRANQLKRDKSQLLAPFDGIISQRQ 190
K + L DE Q S E + L ++ + + K Q D ++R
Sbjct: 163 NKLPELK--LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217



Score = 37.1 bits (86), Expect = 9e-05
Identities = 27/145 (18%), Positives = 51/145 (35%), Gaps = 16/145 (11%)

Query: 101 RLLDAEHQ--EIQASLAQTQADVDLATSTLNR---NLELKKSGYVSEQL--LDENRTQLA 153
+L+ E++ E L ++ ++ S + +L + +E L L + +
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 154 SLEAAKKRLLASQRANQLKRDKSQLLAPFDGIISQRQ-HNLGEVVAAGSPVFTLVGSVNT 212
L N+ ++ S + AP + Q + H G VV + +V +T
Sbjct: 313 LLTLEL-------AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDT 365

Query: 213 -EAYIGVPVAVAQQFVNGQNVTVSV 236
E V GQN + V
Sbjct: 366 LEVTALVQNKDIGFINVGQNAIIKV 390


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1297HTHTETR733e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 73.1 bits (179), Expect = 3e-18
Identities = 40/197 (20%), Positives = 68/197 (34%), Gaps = 5/197 (2%)

Query: 11 RSEQKKQQVLVAAIDLFCRQGFPHTSMDEVAKQAGVSKQTVYSHYGSKDDLFVAAIE--S 68
+++ +Q +L A+ LF +QG TS+ E+AK AGV++ +Y H+ K DLF E
Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67

Query: 69 KCVGHNLNADLLSNPSQPEATLTEFALQFGEMIVSPEAITVFKACVAQSESHP---EVSR 125
+G P P + L E + E V+ E + + V +
Sbjct: 68 SNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQ 127

Query: 126 LFFEAGPQHMLAMLTKYLGAVEALGVYRFSQPHHCAVRLCLMLFGELKLRLELGLETESL 185
+ + L + A + L ++ L
Sbjct: 128 QAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDL 187

Query: 186 LGEREQYIRGCAEMFLK 202
E Y+ EM+L
Sbjct: 188 KKEARDYVAILLEMYLL 204


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1301BONTOXILYSIN330.007 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 32.6 bits (74), Expect = 0.007
Identities = 24/159 (15%), Positives = 56/159 (35%), Gaps = 11/159 (6%)

Query: 418 EITLPPLEKSKLLSDSQFNTIKENDLNKAYFECKKIRNELIKLQY---LITEAKKLASKL 474
+ P +E + + K DLN +E K Y L + S+
Sbjct: 623 NLREPNIEIDDISDSLLGLSFK--DLNNKLYEIYSKNIVYFKKIYFSFLDQWWTEYYSQY 680

Query: 475 NLDSKKSSSTLE-KIDKIELKINKKYKNLSQLIKFYGYFEFSKFLTT---REIESWTQPQ 530
+ ++ + ++ + K+ +LS+ + + T ++ + +Q
Sbjct: 681 FELICMAKQSILAQESLVKQIVQNKFTDLSKASIPPDTLKLIRETTEKTFIDLSNESQIS 740

Query: 531 INQMTERYYEAFLSSCVQVFKLVESACNKLQDRINELKT 569
+N++ +A S CV V + + ++ IN +
Sbjct: 741 MNRVDNFLNKA--SICVFVEDIYPKFISYMEKYINNINI 777


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1302NUCEPIMERASE761e-17 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 75.6 bits (186), Expect = 1e-17
Identities = 41/231 (17%), Positives = 78/231 (33%), Gaps = 54/231 (23%)

Query: 6 TILITGGTGSFGQKYTKTILERY-----------------KPKRLIIFSRDELKQYEMQQ 48
L+TG G G +K +LE K RL + ++ + ++
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHK--- 58

Query: 49 VFNAPCMRYFIGDVRDGDRLKQAFKDVDF--VIHAAALKQVPAAEYNPMECIKTNIHGAE 106
D+ D + + F F V + V + NP +N+ G
Sbjct: 59 -----------IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFL 107

Query: 107 NVIRAAISNNVVKVIALST---------------DKAASPINLYGATKLASDKLFVAANN 151
N++ N + ++ S+ D P++LY ATK A++ + ++
Sbjct: 108 NILEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH 167

Query: 152 VVGDGKTRFAAVRYGNVVGSRGS---VVPFFKQLIANGATSLPITHPDMTR 199
+ G +R+ V G G + F + + G + + M R
Sbjct: 168 LYG---LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKR 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_130560KDINNERMP280.034 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 28.4 bits (63), Expect = 0.034
Identities = 14/39 (35%), Positives = 19/39 (48%), Gaps = 1/39 (2%)

Query: 88 VLKQGSYNNNEPYYLNNISARCPLKGNPGQQLHVDSALP 126
VLK+G Y N Y + N + PL+ + QL LP
Sbjct: 166 VLKRGDYAVNVNYNVQNAGEK-PLEISSFGQLKQSITLP 203


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1315FRAGILYSIN270.041 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 26.6 bits (58), Expect = 0.041
Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 1/48 (2%)

Query: 1 MKRYLFIVAALLLTGCAAK-DKYVQWEDVPPSSFPKLTAIGYAPLATQ 47
+K L + A LL C+ + D D P ++ L ++ Y LATQ
Sbjct: 12 VKLLLMLGTAALLAACSNEADSLTTSIDAPVTASIDLQSVSYTDLATQ 59


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1319HTHFIS633e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.9 bits (153), Expect = 3e-13
Identities = 25/128 (19%), Positives = 53/128 (41%), Gaps = 12/128 (9%)

Query: 180 HIMVIDDSAVARKQIIRALESLNLQIDTAKDGREALDKLKAIAGEMNNVAEEIPLIISDI 239
I+V DD A R + +AL + + + A G+ L+++D+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGD---------LVVTDV 55

Query: 240 EMPEMDGYTLTAEIRDDPKLKHIKVVLHTSLSGVFNQAMVQKVGANDFIAK-FNPDELAA 298
MP+ + + L I+ + V++ ++ + + GA D++ K F+ EL
Sbjct: 56 VMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG 113

Query: 299 AVNKHLSL 306
+ + L+
Sbjct: 114 IIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1322FLGHOOKAP1333e-04 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 32.6 bits (74), Expect = 3e-04
Identities = 9/38 (23%), Positives = 18/38 (47%)

Query: 99 NVNVMEEMADMISASRSYQMNVQVAEAAKSMLQQTLGM 136
VN+ EE ++ + Y N QV + A ++ + +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 28.4 bits (63), Expect = 0.011
Identities = 15/64 (23%), Positives = 27/64 (42%), Gaps = 6/64 (9%)

Query: 8 DVAGSGMSAQSLRLNTTASNIANADSVSSSVDKTYRSRHPIFEAEMAKAQSQQQASQGVT 67
+ A SG++A LNT ++NI++ + Y + I + + GV
Sbjct: 5 NNAMSGLNAAQAALNTASNNISSYN------VAGYTRQTTIMAQANSTLGAGGWVGNGVY 58

Query: 68 VKGI 71
V G+
Sbjct: 59 VSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1324FLGHOOKAP1401e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 40.3 bits (94), Expect = 1e-05
Identities = 15/35 (42%), Positives = 22/35 (62%)

Query: 2 SFNIALSGISAAQKDLNTTANNIANANTIGFKESR 36
N A+SG++AAQ LNT +NNI++ N G+
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37



Score = 37.2 bits (86), Expect = 1e-04
Identities = 13/49 (26%), Positives = 25/49 (51%)

Query: 405 SLSSSALEQSNIDLTTELVDLISAQRNFQANSRTLEVNNTLQQTVLQIR 453
LS+ S ++L E +L Q+ + AN++ L+ N + ++ IR
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1326FLGHOOKAP1452e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.6 bits (105), Expect = 2e-07
Identities = 19/119 (15%), Positives = 41/119 (34%), Gaps = 4/119 (3%)

Query: 145 EDATSITVSAEGEVSVKTPGAAENQVVGQLSMTDFINPSGLDPMGQNLYTETG---ASGT 201
D I +++E + + + Q + + +L ++ G A+
Sbjct: 427 TDEAKIAMASEEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLK 486

Query: 202 PIQGTASLDGMGAIRQGALETSNVNVTEELVNLIESQRIYEMNSKVISAVDQMLAYVNQ 260
T + + S VN+ EE NL Q+ Y N++V+ + + +
Sbjct: 487 TSSATQGNV-VTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544



Score = 35.7 bits (82), Expect = 2e-04
Identities = 9/36 (25%), Positives = 20/36 (55%)

Query: 5 LWISKTGLDAQQTDIAVISNNVANASTVGYKKSRAV 40
+ + +GL+A Q + SNN+++ + GY + +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI 39


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1327FLGLRINGFLGH1472e-46 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 147 bits (373), Expect = 2e-46
Identities = 75/215 (34%), Positives = 106/215 (49%), Gaps = 9/215 (4%)

Query: 11 LLLAACSSTPKKPIADDPFYAPVYPEAPPTKIAATGSIYQDSQAA-----SLYSDIRAHK 65
L L C+ P P+ A P P A GSI+Q +Q L+ D R
Sbjct: 17 LSLTGCAWIPSTPLVQGATSAQPVPGPTP---VANGSIFQSAQPINYGYQPLFEDRRPRN 73

Query: 66 VGDIITIVLKEATQAKKSAGNQIKKGSDMTLDPIYAGGSNVSL-GGIPLDLRYKDSMNTK 124
+GD +TIVL+E A KS+ + L G D+
Sbjct: 74 IGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGGNTFN 133

Query: 125 RESDADQSNSLDGSISANIMQVLNNGNLVVRGEKWISINNGDEFIRVTGIVRSQDIKPDN 184
+ A+ SN+ G+++ + QVL NGNL V GEK I+IN G EFIR +G+V + I N
Sbjct: 134 GKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTISGSN 193

Query: 185 TIDSTRMANARIQYSGTGTFAEAQKVGWLSQFFMS 219
T+ ST++A+ARI+Y G G EAQ +GWL +FF++
Sbjct: 194 TVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLN 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1328FLGPRINGFLGI370e-130 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 370 bits (952), Expect = e-130
Identities = 157/367 (42%), Positives = 222/367 (60%), Gaps = 14/367 (3%)

Query: 5 LIVALAMLVLSLPSQAE--RIKDIANVQGVRSNQLIGYGLVVGLPGTGEK---TNYTEQT 59
L+ + + + P+QA+ RIKDIA++Q R NQLIGYGLVVGL GTG+ + +TEQ+
Sbjct: 11 LVFSALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQS 70

Query: 60 FTTMLKNFGINLPDNFRPKIKNVAVVAVHADMPAFIKPGQQLDVTVSSLGEAKSLRGGTL 119
ML+N GI + KN+A V V A++P F PG ++DVTVSSLG+A SLRGG L
Sbjct: 71 MRAMLQNLGITTQGG-QSNAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNL 129

Query: 120 LQTFLKGVDGNVYAIAQGSLVVSGFSAEGLDGSKVIQNTPTVGRIPNGAIVERSVATPFS 179
+ T L G DG +YA+AQG+L+V+GFSA+G D + + Q T R+PNGAI+ER + + F
Sbjct: 130 IMTSLSGADGQIYAVAQGALIVNGFSAQG-DAATLTQGVTTSARVPNGAIIERELPSKFK 188

Query: 180 SGDYLTFNLRRADFSTAQRMADAINDL----LGPDMARPLDATSVQVSAPRDVSQRVSFL 235
L LR DFSTA R+AD +N G +A P D+ + V PR V+ +
Sbjct: 189 DSVNLVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPR-VADLTRLM 247

Query: 236 ATLENLDVIPAEESAKVIVNSRTGTIVVGQHVKLLPAAVTHGGLTVTIAEATQVSQPNAL 295
A +ENL + + AKV++N RTGTIV+G V++ AV++G LTV + E+ QV QP
Sbjct: 248 AEIENL-TVETDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPF 306

Query: 296 ANGETVVTANTTIGVNESDRRMFMFSPGTTLDELVRAVNLVGAAPSDVLAILEALKVAGA 355
+ G+T V T I + ++ G L LV +N +G ++AIL+ +K AGA
Sbjct: 307 SRGQTAVQPQTDIMAMQEGSKVA-IVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGA 365

Query: 356 LHGELII 362
L EL++
Sbjct: 366 LQAELVL 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1329FLGFLGJ1492e-44 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 149 bits (378), Expect = 2e-44
Identities = 67/160 (41%), Positives = 95/160 (59%), Gaps = 2/160 (1%)

Query: 219 RETQKTLKFGSREEFLATLYPHAEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPS 278
R +L S+ FLA L A+ A++ G ++LAQ+ALE+GWGQ+ +R NG PS
Sbjct: 139 RNYDDSLPGDSKA-FLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPS 197

Query: 279 HNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDFEHSFNDFVTFIAEGERYQ 338
+NLF +KA W G ++T E+E G A + KA FRVY+ + + +D+V + RY
Sbjct: 198 YNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYA 257

Query: 339 DAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQTISQ 378
A AAS Q +ALQDAGYATDP YA K+ ++Q +
Sbjct: 258 -AVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKS 296



Score = 87.8 bits (217), Expect = 1e-21
Identities = 39/93 (41%), Positives = 61/93 (65%), Gaps = 3/93 (3%)

Query: 12 DLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFY 71
D L+ L+A+A +D ++ VA+Q EG+FVQM++KSMRDA D +S++T+ Y
Sbjct: 14 DAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALP---KDGLFSSEHTRLY 70

Query: 72 EQMRDQQLSVDLSDKGVLGLADMMVQQLSPESS 104
M DQQ++ ++ LGLA+MMV+Q++PE
Sbjct: 71 TSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQP 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1330FLGHOOKAP12196e-66 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 219 bits (560), Expect = 6e-66
Identities = 127/460 (27%), Positives = 193/460 (41%), Gaps = 29/460 (6%)

Query: 4 DLLNIARTGVLASQSQLGVTSNNIANANTAGYHRQVATQSTLESQRLGNSFYGTGTYVDD 63
L+N A +G+ A+Q+ L SNNI++ N AGY RQ + S + G G YV
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSG 61

Query: 64 VKRIYNDYAARELRIGQTTLSGAEASYGKLSELDQLFSQIGKMVPQSLNSLFTGLNSLAD 123
V+R Y+ + +LR QT SG A Y ++S++D + S + + FT L +L
Sbjct: 62 VQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVS 121

Query: 124 LPADLGIRSSTLNDAKQLANSLNQMQSSLNGQMTQTNDQITGMTKRINEISKELANLNLE 183
D R + + ++ L N L Q Q N I +IN +K++A+LN +
Sbjct: 122 NAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLNDQ 181

Query: 184 LMKSPNQDAM-----LLDKQDALIQELSQYAQVNVIPQENGAKSIMLGGSVMLVSGEIAM 238
+ + A LLD++D L+ EL+Q V V Q+ G +I + LV G A
Sbjct: 182 ISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTAR 241

Query: 239 TMDTKTGDPFPNELQLTSSIGSQSVAADPSKL--GGQLGALFEYRDQTLIPASHELDQLA 296
+ P+ + G+ P KL G LG + +R Q L + L QLA
Sbjct: 242 QLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLA 301

Query: 297 LGIADNFNKMQAQGFDLNGQVGSNIFRDINDPLMSLGRVGGYSNNTGNATLGVNIDDTRL 356
L A+ FN GFD NG G + F + V + N G+ +G + D
Sbjct: 302 LAFAEAFNTQHKAGFDANGDAGEDFFA------IGKPAVLQNTKNKGDVAIGATVTDASA 355

Query: 357 LTGGSYELSF-------TAPASYELRDTETGVITPLTLNGSTLEGGAGFSIDIKAGAMAS 409
+ Y++SF T AS + +G L G A
Sbjct: 356 VLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFT---------GTPAV 406

Query: 410 GDRFVIRPTAGAANGITVEMTDPKGIAAASPKITADAANS 449
D F ++P + A + V +TD IA AS + D+ N
Sbjct: 407 NDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNR 446



Score = 89.6 bits (222), Expect = 8e-21
Identities = 38/103 (36%), Positives = 55/103 (53%)

Query: 535 AEGDNSNAVAMAKLSESKVMNGGKSTLADVFENTKIDIGSKTKAAEVRVGSAEAIYQQAY 594
+ DN N A+ L + GG + D + + DIG+KT + + + Q
Sbjct: 441 GDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLS 500

Query: 595 ARVESESGVNLDEEAANLMRFQQAYQASARIMTTAQQIFDTLL 637
+ +S SGVNLDEE NL RFQQ Y A+A+++ TA IFD L+
Sbjct: 501 NQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALI 543


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1331FLAGELLIN582e-11 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 58.1 bits (140), Expect = 2e-11
Identities = 63/359 (17%), Positives = 119/359 (33%), Gaps = 8/359 (2%)

Query: 20 QTATSKILEQLSSGKKVNTAGDDPVAALGIDNLNQRNALVDQFMKNIDYATNRLAVTESK 79
Q++ S +E+LSSG ++N+A DD + + Q +N + + TE
Sbjct: 21 QSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNANDGISIAQTTEGA 80

Query: 80 LGSAENLASSIREQVMRAVNGTLADSERQMIADEMKGSLEELLSIANSKDESGNYMFSGY 139
L N +RE ++A NGT +DS+ + I DE++ LEE+ ++N +G + S
Sbjct: 81 LNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKVLSQ- 139

Query: 140 STDKEPFAFDNSTPPKIVYSGDSGIRNSLVQSGVALGTNVPGDTAFMKAPNGLGDYSVNY 199
+ N + ++ V+S G NV G +V
Sbjct: 140 DNQMKIQVGANDGETITID-----LQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTG 194

Query: 200 LASQQGEFSVKTAKIADPATYLADTYTFNFSDNGSGGTNLQVLDSANNPVANIANFDAAT 259
+ + + A T N Q+ + F
Sbjct: 195 YDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTK 254

Query: 260 PVSFNGIEVNISGKPSAGDSFTMEPQSEVSIFDTISRAIALIEDPNSANTPQGRSQLAQI 319
+ I+G G V+ TI + + T G +
Sbjct: 255 STAGTAEAKAIAGAIKGGKEGDTFDYKGVTF--TIDTKTGNDGNGKVSTTINGEKVTLTV 312

Query: 320 LNDIDSGVNQISSARSVAGNNLKAVESYKDTHIEEQVLNTSALSLLEDLDYASAITEFA 378
+ N ++ + N +V + + T ++ ++ LS LE + ++
Sbjct: 313 ADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKIT 371


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1332FLAGELLIN1344e-38 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 134 bits (337), Expect = 4e-38
Identities = 93/271 (34%), Positives = 125/271 (46%), Gaps = 10/271 (3%)

Query: 2 AITVNTNVTSMKAQKNLNTSSSGLATSMERLSSGLRINSAKDDAAGLAISNRLNSQVRGL 61
A +NTN S+ Q NLN S S L++++ERLSSGLRINSAKDDAAG AI+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTVQAENGANSTDDLDAIQKEINQLSE 121
RNAND ISIAQ EGA+ E N LQR+R+L+VQA NG NS DL +IQ EI Q E
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EITAIGDSTAFGNTLLMTGLFSTGKTFQVGHQEGEDITISVGTTNAGSL--------SVN 173
EI + + T F +++ QVG +GE ITI + + SL
Sbjct: 121 EIDRVSNQTQFNGVKVLSQ--DNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPK 178

Query: 174 ALAIASAGGRSTALANIDAAIKTIDNQRANLGAKQNRLAYNISNSANTQANVADAKSRIV 233
+ + D + R ++ + + A
Sbjct: 179 EATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTT 238

Query: 234 DVDFAKETSVMTKNQVLQQTGSAMLAQANQL 264
D + K + A A +
Sbjct: 239 DDAENNTAVDLFKTTKSTAGTAEAKAIAGAI 269



Score = 85.9 bits (212), Expect = 4e-21
Identities = 64/213 (30%), Positives = 99/213 (46%), Gaps = 4/213 (1%)

Query: 60 GLDVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTVQAENGANSTDDLDAIQKEINQL 119
+ + +++A I GA LQ +++ NG + DD +
Sbjct: 298 KVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSD 357

Query: 120 SEEITAIGDSTAFGNTLLMTGLFSTGKTFQVGHQEGEDITISVGTTNAGSLSVNALAIAS 179
E A+ + + G + + T + S +N A A+
Sbjct: 358 LEANNAVKGESKITVNGAEYTANAAGDKVTLAGK----TMFIDKTASGVSTLINEDAAAA 413

Query: 180 AGGRSTALANIDAAIKTIDNQRANLGAKQNRLAYNISNSANTQANVADAKSRIVDVDFAK 239
+ LA+ID+A+ +D R++LGA QNR I+N NT N+ A+SRI D D+A
Sbjct: 414 KKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYAT 473

Query: 240 ETSVMTKNQVLQQTGSAMLAQANQLPQVALSLL 272
E S M+K Q+LQQ G+++LAQANQ+PQ LSLL
Sbjct: 474 EVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1333FLAGELLIN1395e-40 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 139 bits (350), Expect = 5e-40
Identities = 98/270 (36%), Positives = 129/270 (47%), Gaps = 10/270 (3%)

Query: 2 AITVNTNVTSLKAQKNLNTSASGLATSMERLSSGLRINSAKDDAAGLAISNRLNSQVRGL 61
A +NTN SL Q NLN S S L++++ERLSSGLRINSAKDDAAG AI+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 EVGMRNANDAISIAQISEGAMQEQTNMLQRMRDLTVQSENGANSTDDLDAIQKEIDQLAL 121
RNAND ISIAQ +EGA+ E N LQR+R+L+VQ+ NG NS DL +IQ EI Q
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EITEIGDNTAFGSTKLLDGTFSGKTFQVGHQSGEDITISVAKTTASALKVDSLDITGSAR 181
EI + + T F K+L QVG GE ITI + K +L +D ++ G
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQ-MKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 ASALAA---------IDAAIKTIDSQRADLGAKQNRLAYNISNSANTQANIADAKSRIVD 232
A+ D + R D+ + + A D
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 233 VDFAKETSQMTKNQVLQQTGSAMLAQANQL 262
+ K + A A +
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAI 269



Score = 84.3 bits (208), Expect = 1e-20
Identities = 63/212 (29%), Positives = 101/212 (47%), Gaps = 4/212 (1%)

Query: 60 GLEVGMRNANDAISIAQISEGAMQEQTNMLQRMRDLTVQSENGANSTDDLDAIQKEIDQ- 118
+ + +++A I+ GA LQ +++ NG + DD +
Sbjct: 298 KVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSD 357

Query: 119 LALEITEIGDNTAFGSTKLLDGTFSGKTFQVGHQSGEDITISVAKTTASALKVDSLDITG 178
L G++ + +G + ++ I + S L +
Sbjct: 358 LEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTM---FIDKTASGVSTLINEDAAAAK 414

Query: 179 SARASALAAIDAAIKTIDSQRADLGAKQNRLAYNISNSANTQANIADAKSRIVDVDFAKE 238
+ A+ LA+ID+A+ +D+ R+ LGA QNR I+N NT N+ A+SRI D D+A E
Sbjct: 415 KSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATE 474

Query: 239 TSQMTKNQVLQQTGSAMLAQANQLPQVALSLL 270
S M+K Q+LQQ G+++LAQANQ+PQ LSLL
Sbjct: 475 VSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


15Shewana3_1366Shewana3_1393Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1366023-3.090343CheW protein
Shewana3_1367-119-3.307525CheW protein
Shewana3_1368-116-2.941543hypothetical protein
Shewana3_1369-117-3.328588FlhB domain-containing protein
Shewana3_1370-121-3.769369hypothetical protein
Shewana3_1371-123-4.621814VacJ family lipoprotein
Shewana3_1372-225-5.613844response regulator receiver protein
Shewana3_1373-130-6.936839amino acid/peptide transporter
Shewana3_1374-134-8.137045transcription antitermination protein nusG
Shewana3_1375-136-8.381855polysaccharide export protein
Shewana3_1376139-9.666672lipopolysaccharide biosynthesis protein
Shewana3_1377242-10.474023sugar transferase
Shewana3_1378245-12.720968glycosyl transferase family protein
Shewana3_1379146-13.283043dTDP-glucose-4,6-dehydratase
Shewana3_1380251-15.271802glucose-1-phosphate thymidylyltransferase
Shewana3_1381354-17.037418dTDP-4-dehydrorhamnose reductase
Shewana3_1382157-17.764369dTDP-4-dehydrorhamnose 3,5-epimerase
Shewana3_1383151-16.208616hypothetical protein
Shewana3_1384147-13.933770hypothetical protein
Shewana3_1385037-10.715097hypothetical protein
Shewana3_1386133-8.588845glycosyl transferase family protein
Shewana3_1387-127-6.487527UDP-glucose/GDP-mannose dehydrogenase
Shewana3_1388023-5.135167hypothetical protein
Shewana3_1389025-5.409724lipoprotein
Shewana3_1390025-5.575826GAF sensor signal transduction histidine kinase
Shewana3_1391229-5.637341glucose-1-phosphate thymidylyltransferase
Shewana3_1392225-5.133840dTDP-4-dehydrorhamnose 3,5-epimerase
Shewana3_1393020-3.843877hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1369TYPE3IMSPROT522e-11 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 52.5 bits (126), Expect = 2e-11
Identities = 17/87 (19%), Positives = 30/87 (34%), Gaps = 3/87 (3%)

Query: 8 TQQAVALSYDGKN--APKVVASGEGLVADEIIALAKASGVFIHQDPHLSNFL-RLLELGE 64
T A+ + Y P V + +A+ GV I Q L+ L +
Sbjct: 265 THIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDH 324

Query: 65 EIPKELYLLIAELIAFVYMLDGKFPEQ 91
IP E AE++ ++ + +
Sbjct: 325 YIPAEQIEATAEVLRWLERQNIEKQHS 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1371VACJLIPOPROT2292e-77 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 229 bits (584), Expect = 2e-77
Identities = 87/222 (39%), Positives = 126/222 (56%), Gaps = 4/222 (1%)

Query: 44 PRDPFEGFNRAMWDFNYLYLDRYIYRPVAHGYNDYLPLPAKTGINNFVQNLEEPSSLVNN 103
DP EGFNR M++FN+ LD YI RPVA + DY+P PA+ G++NF NLEEP+ +VN
Sbjct: 28 RSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNY 87

Query: 104 ALQGKWGWAANAGGRFTVNTTIGLLGVFDVADMMGMPRKQDE---FNEVLGYYGVPNGPY 160
LQG RF +NT +G+ G DVA M ++ E F LG+YGV GPY
Sbjct: 88 FLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPY 147

Query: 161 FMAPFAGPYVVRELASDWVDGLYFPLSELTVWQSIVKWGLKSLHARASAIDQERLVDNAL 220
PF G + +R+ D D LY LS LT S+ KW L+ + RA +D + L+ +
Sbjct: 148 VQLPFYGSFTLRDDGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSS 207

Query: 221 DPYTFVKDAYLQHMDYKVYDGNV-PQKQEDDELLDQYMQELE 261
DPY V++AY Q D+ G + PQ+ + + + +++++
Sbjct: 208 DPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQDDLKDID 249


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1372HTHFIS884e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 87.6 bits (217), Expect = 4e-21
Identities = 28/101 (27%), Positives = 44/101 (43%)

Query: 7 SILWVEDDPVFRQIVATFLIGRGAKVVQACDGEQGLYIFKQQRFDIILADLSMPKLGGLD 66
+IL +DD R ++ L G V + D+++ D+ MP D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 67 MLKEMSKLEPLVPSIVVSGNNVMADVVEALRVGACDYLVKP 107
+L + K P +P +V+S N ++A GA DYL KP
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKP 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1379NUCEPIMERASE1754e-54 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 175 bits (445), Expect = 4e-54
Identities = 83/361 (22%), Positives = 149/361 (41%), Gaps = 51/361 (14%)

Query: 1 MKILVTGGAGFIGSAVIRHIIMNTNDSVINVDKLT--YAGNLESLKL-VSTNPRYNFEQV 57
MK LVTG AGFIG V + ++ + V+ +D L Y +L+ +L + P + F ++
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQ-VVGIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59

Query: 58 DICDRATLERVFSQYQPDAVMHLAAESHVDRSITGPSDFIQTNIVGTYILLEAARQYWTQ 117
D+ DR + +F+ + V V S+ P + +N+ G +LE R Q
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 118 LDAERKAAFRFHHISTDEVYGDLPHPDEQEGQAVNQYLPLFTETTPYAPSSPYSASKASS 177
+ S+ VYG N+ +P T+ + P S Y+A+K ++
Sbjct: 120 ---------HLLYASSSSVYGL------------NRKMPFSTDDSVDHPVSLYAATKKAN 158

Query: 178 DHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKSLPIYGKGDQIRDWL 237
+ + + YG P YGP+ P+ + LEGKS+ +Y G RD+
Sbjct: 159 ELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFT 218

Query: 238 YVEDHARALYKVV------------------TEGKVGETYNIGGHNEKQNIEVVKTICSI 279
Y++D A A+ ++ YNIG +E++ I ++
Sbjct: 219 YIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNS---SPVELMDYIQAL 275

Query: 280 LDSLVPKATPYAEQITFVTDRPGHDRRYAIDASKMSAELNWQPQETFETGLRKTIEWYLA 339
D+L +A + + +PG + D + + + P+ T + G++ + WY
Sbjct: 276 EDALGIEA-----KKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRD 330

Query: 340 N 340

Sbjct: 331 F 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1381NUCEPIMERASE503e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 50.2 bits (120), Expect = 3e-09
Identities = 32/161 (19%), Positives = 58/161 (36%), Gaps = 27/161 (16%)

Query: 1 MKILVTGSNGQVGSCLVKLLNQIPEIEFLAVD--------------REQL---------- 36
MK LVTG+ G +G + K L + + + +D E L
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGH-QVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59

Query: 37 DITDYEAVNKLVSEFKPDAIINAAAHTAVDKAEQEVELSYAINRDGPQFLAQAANSVG-A 95
D+ D E + L + + + + AV + + N G + +
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 96 TILHISTDYVFAGDKVGEYVETDEVA-PQGIYGKSKLAGEL 135
+L+ S+ V+ ++ + D V P +Y +K A EL
Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1389PF06291300.002 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 29.6 bits (66), Expect = 0.002
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)

Query: 19 MKLSQISLALLALMITACSEPAKTVANEPVAAPHQDTQTN 58
MK S AL A++IT C++ TV N+P A ++T T+
Sbjct: 6 MKKMLFSAAL-AMLITGCAQQTFTVGNKPTAVTPKETITH 44


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1390PF06580478e-08 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 46.8 bits (111), Expect = 8e-08
Identities = 36/197 (18%), Positives = 75/197 (38%), Gaps = 36/197 (18%)

Query: 266 NTMQDGLGLIERNLSRAAELV--------HNFKRTAADQSVLERERFNLKTYIFQIFSSL 317
N + + LI + ++A E++ ++ + + A Q L E + +Y+ + S
Sbjct: 177 NALNNIRALILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQ-LAS-- 233

Query: 318 RPLMR-KKNIVLNVELDDDIFIESYPGAIAQIFTNLVANSFRHGFPESFTGDKSITIRVQ 376
++ + + +++ I P + Q LV N +HG + G K I ++
Sbjct: 234 ---IQFEDRLQFENQINPAIMDVQVPPMLVQT---LVENGIKHGIAQLPQGGK-ILLKGT 286

Query: 377 KQDSNICMQYQDNGVGMSDEVKLKAFEPFFTTARKDGGTGLGMSIIYNLVTQKLHGTI-- 434
K + + ++ ++ G K TG G+ + + Q L+GT
Sbjct: 287 KDNGTVTLEVENTGSLALKNTK--------------ESTGTGLQNVRERL-QMLYGTEAQ 331

Query: 435 LLASSPDQGVKVEIQIP 451
+ S V + IP
Sbjct: 332 IKLSEKQGKVNAMVLIP 348


16Shewana3_1433Shewana3_1440Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_14334260.447265hypothetical protein
Shewana3_14343250.834283S-adenosylmethionine--tRNA
Shewana3_14353280.601276queuine tRNA-ribosyltransferase
Shewana3_14360241.756446protein translocase subunit yajC
Shewana3_14370202.337114preprotein translocase subunit SecD
Shewana3_14383162.248453preprotein translocase subunit SecF
Shewana3_14392162.831884hypothetical protein
Shewana3_14402152.575522rhodanese domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1437SECFTRNLCASE802e-18 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 79.9 bits (197), Expect = 2e-18
Identities = 32/165 (19%), Positives = 81/165 (49%), Gaps = 4/165 (2%)

Query: 434 VSIVEERTIGPSLGAENIESGVQAMIWGMAVVLIFMLVYYR-SFGLIANLALTANLVMVV 492
+ I ++GP + E + + V +++ V++ ++ V + F L A +AL ++++ V
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 493 GVMSMIPGAVLTLPGIAGMVLTVGMAVDGNVLIYERIREELRA--GRSVQQAIHEGYGNA 550
G+ +++ L +A ++ G +++ V++++R+RE L ++ ++
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 551 FSTIADANITTFLTALILFAVGTGAIKGFAVTLMIGIATSMFTAI 595
S +TT L + + G I+GF ++ G+ T ++++
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSV 298


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1438SECFTRNLCASE314e-109 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 314 bits (807), Expect = e-109
Identities = 111/309 (35%), Positives = 178/309 (57%), Gaps = 14/309 (4%)

Query: 2 LEILSLKRTVNFLRHALPISIMSAILVFGSLVSLATKGINWGLDFTGGTVVEMEFTQPVD 61
L+++ K +F R + +++ S++ G+N+G+DF GGT + E T +D
Sbjct: 5 LKLVPEKTNFDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAID 64

Query: 62 LNVLRTKLSAPELDGAVVQNFGSSR------DVLVRLSVKE--------GVSSDVQVKSV 107
+ V R L EL ++ ++R+ ++E G V V
Sbjct: 65 VGVYRAALEPLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKV 124

Query: 108 MAAAQQVDAGVQQKRVEFVGPQVGKELAEQGGLAVLVALICIMIYVSFRFEWRLAFGSVA 167
A VD ++ E VGP+V EL ++L A + IM Y+ RFEW+ A G+V
Sbjct: 125 ETALTAVDPALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVV 184

Query: 168 ALAHDVIVTLGVFSVFQLEFDLTVLAGVLTVVGYSLNDTIVVFDRIRENFLKMRKSEPEE 227
AL HDV++T+G+F+V QL+FDLT +A +LT+ GYS+NDT+VVFDR+REN +K + +
Sbjct: 185 ALVHDVLLTVGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRD 244

Query: 228 VVNVSITQTMSRTIITTGTTLVTVVALFLKGGTMIHGFATALLLGIFVGTYSSIYVASYL 287
V+N+S+ +T+SRT++T TTL+ +V + + GG +I GF A++ G+F GTYSS+YVA +
Sbjct: 245 VMNLSVNETLSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNI 304

Query: 288 AIKLGICRE 296
+ +G+ R
Sbjct: 305 VLFIGLDRN 313


17Shewana3_1452Shewana3_1464Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_14522152.588959hypothetical protein
Shewana3_14531143.236004RNA polymerase sigma factor
Shewana3_14541143.556046hypothetical protein
Shewana3_14551122.831640hypothetical protein
Shewana3_14560121.576155von Willebrand factor type A domain-containing
Shewana3_14570111.479755hypothetical protein
Shewana3_1458-1140.685521hypothetical protein
Shewana3_1459023-5.555183ATPase
Shewana3_1460-120-5.5409863-ketoacyl-CoA thiolase
Shewana3_1461-122-6.208280multifunctional fatty acid oxidation complex
Shewana3_1462023-7.186395hypothetical protein
Shewana3_1463-121-6.689367hypothetical protein
Shewana3_1464-118-5.673789PAS/PAC sensor-containing diguanylate
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1455IGASERPTASE572e-10 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 57.4 bits (138), Expect = 2e-10
Identities = 46/269 (17%), Positives = 96/269 (35%), Gaps = 22/269 (8%)

Query: 406 NGLYNQGNALMQLGKPDKAKERYQAALDKQPDFPQAKANLELAEKLLNQ----------- 454
NG Y+ N ++ + Q D P +N E ++
Sbjct: 975 NGRYDLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPS 1034

Query: 455 QQSQQNADNQEKQSQGDENQQGQNQNDQQQGQNQQQGQQGDQQSSQNDQAQDQSQEQQSQ 514
+ ++ A+N +++S+ E + + QN++ ++ N Q + +Q
Sbjct: 1035 ETTETVAENSKQESKTVEKN--EQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSET 1092

Query: 515 QQNNPDQADEKPSQEQESSSEQSNLEQGAQDKQQASDDKAKQDQQDAQQEQQQAEQQANQ 574
++ + E + E+E + + E+ + + S KQ+Q + Q Q + +
Sbjct: 1093 KETQTTETKETATVEKEEKA-KVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPA----R 1147

Query: 575 QNRADNNAEDKEEQASNEAKMQAQVEDDKSKAEQEQQQAVAQKADKEKQSQADKNPDTAI 634
+N N ++ + Q + A + ++ S EQ V + + +NP+
Sbjct: 1148 ENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQP----VTESTTVNTGNSVVENPENTT 1203

Query: 635 ESVEAPPSNSEPLPAEMQRALRGVSEDPQ 663
+ P NSE R R V P
Sbjct: 1204 PATTQPTVNSESSNKPKNRHRRSVRSVPH 1232



Score = 54.7 bits (131), Expect = 1e-09
Identities = 35/189 (18%), Positives = 70/189 (37%), Gaps = 3/189 (1%)

Query: 495 DQQSSQNDQAQDQSQEQQSQQQNNPDQADEKPSQEQESSSEQSNLEQGAQDKQQASDDKA 554
+ Q+ + + + + P A PS+ E+ +E S E +K + +
Sbjct: 1002 NIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATET 1061

Query: 555 KQDQQDAQQEQQQAEQQANQQNRADNNAEDKEEQASNEAKMQAQVE-DDKSKAEQEQQQA 613
++ +E + + Q N + + +E + E K A VE ++K+K E E+ Q
Sbjct: 1062 TAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQE 1121

Query: 614 VAQKADKE--KQSQADKNPDTAIESVEAPPSNSEPLPAEMQRALRGVSEDPQVLLRNKMQ 671
V + + KQ Q++ A + E P+ + P + + N Q
Sbjct: 1122 VPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQ 1181

Query: 672 LEYQKRRQN 680
+ N
Sbjct: 1182 PVTESTTVN 1190



Score = 34.3 bits (78), Expect = 0.002
Identities = 38/269 (14%), Positives = 79/269 (29%), Gaps = 20/269 (7%)

Query: 360 AMQAYQAEDYANAAQKFETPQWQGAAQYKAGEYEQALKNFEQDSSANGLYNQGNALMQLG 419
+ + + K Q A + A E A + + AN Q N + Q G
Sbjct: 1034 SETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAK-SNVKAN---TQTNEVAQSG 1089

Query: 420 KPDKAKERYQAALDKQPDFPQAKANLELAEKLLNQQQSQQNADNQEKQSQGDENQQGQNQ 479
K + + + KA +E + + + Q + QE+ + +
Sbjct: 1090 SETKETQT-TETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARE 1148

Query: 480 NDQQQGQNQQQGQQGDQQSSQN-----------DQAQDQSQEQQSQQQNNPDQADE---K 525
ND + Q Q ++ + + + NP+ +
Sbjct: 1149 NDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQ 1208

Query: 526 PSQEQESSSEQSNLEQGAQDKQQASDDKAKQDQQDAQQEQQQAEQQANQQNRADNNAEDK 585
P+ ESS++ N + + + + A D + + N ++A K
Sbjct: 1209 PTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTV-ALCDLTSTNTNAVLSDARAK 1267

Query: 586 EEQASNEAKMQAQVEDDKSKAEQEQQQAV 614
+ + + + E Q V
Sbjct: 1268 AQFVALNVGKAVSQHISQLEMNNEGQYNV 1296


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1459HTHFIS348e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 33.7 bits (77), Expect = 8e-04
Identities = 35/139 (25%), Positives = 53/139 (38%), Gaps = 19/139 (13%)

Query: 28 LIALIANG--HLLVEGPPGLAKT---RAVKALCDGVEGDFHRIQ---FTPDLLPADLTG- 78
++A + L++ G G K RA+ G F I DL+ ++L G
Sbjct: 152 VLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGH 211

Query: 79 -----TDIYRSQTGTFEFEAGPIFHNLILADEINRAPAKVQSALLEAMAEGQVT-VGKNS 132
T TG FE G + DEI P Q+ LL + +G+ T VG +
Sbjct: 212 EKGAFTGAQTRSTGRFEQAEG----GTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRT 267

Query: 133 YKLPPLFLVMATQNPLENE 151
+ +V AT L+
Sbjct: 268 PIRSDVRIVAATNKDLKQS 286


18Shewana3_1607Shewana3_1619Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1607017-4.869559ATP-dependent protease
Shewana3_1608-133-9.181602**response regulator
Shewana3_1609-134-9.559702excinuclease ABC subunit C
Shewana3_1610247-12.924398CDP-diacylglycerol--glycerol-3-phosphate
Shewana3_1611549-13.789238****hypothetical protein
Shewana3_1612547-13.589546hypothetical protein
Shewana3_1613538-10.971542rRNA (guanine-N(1)-)-methyltransferase
Shewana3_1615327-7.994305histone family protein DNA-binding protein
Shewana3_1616326-7.458517hypothetical protein
Shewana3_1617222-5.702257hypothetical protein
Shewana3_1618117-4.517934ECF subfamily RNA polymerase sigma-24 factor
Shewana3_1619013-3.430387serine/threonine protein kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1608HTHFIS792e-19 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 79.1 bits (195), Expect = 2e-19
Identities = 30/144 (20%), Positives = 59/144 (40%), Gaps = 6/144 (4%)

Query: 2 ISIYLVDDHELVRTGIRRILEDERGIKVVGEAPDGETAVQWARQNEADVILMDMNMPGMG 61
+I + DD +RT + + L G V + + T +W + D+++ D+ MP
Sbjct: 4 ATILVADDDAAIRTVLNQALSRA-GYDVRITS-NAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 62 GLEATRKILRYQPHAKIIVLTVHTEDPFPSKVMQAGASGYLTKGATPPEVL----QAIRQ 117
+ +I + +P ++V++ K + GA YL K E++ +A+ +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 118 VSRGQRYLSPEIAQQMALSQFNPA 141
R L + M L + A
Sbjct: 122 PKRRPSKLEDDSQDGMPLVGRSAA 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1613SUBTILISIN1191e-31 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 119 bits (299), Expect = 1e-31
Identities = 60/247 (24%), Positives = 102/247 (41%), Gaps = 50/247 (20%)

Query: 251 FVYNEQTKLNDPTEYKICINGHGLSVASSIAAIQNNGKGIASAVGSENIDIVPVKVIDSC 310
F +++ +Y NGHG VA +IAA +N + A ++ ++ +KV++
Sbjct: 69 FTDDDEGDPEIFKDY----NGHGTHVAGTIAATENENGVVGVAPEAD---LLIIKVLNKQ 121

Query: 311 TGSALTSDLIKAIYWAAKSDDTFEGLEPISEPVDVINLSLGSNKNELCEVGYNAFADAVD 370
GS +I+ IY+A + VD+I++SLG + +AV
Sbjct: 122 -GSGQYDWIIQGIYYAIEQK------------VDIISMSLGGPE------DVPELHEAVK 162

Query: 371 YAKQKGIVVVAALGNDGVSGD----IFTPATCNGVIPVSSNNVHGQLSYFSSYLSDKRTL 426
A I+V+ A GN+G D + P N VI V + N S FS+ ++ L
Sbjct: 163 KAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVISVGAINFDRHASEFSNSNNEV-DL 221

Query: 427 STIGEDMTLPKVTTTTYIDRNFIDTNCQGSIESCYATGQGTSYSAPIVSGLVSMVLMQNP 486
GED+ +T YAT GTS + P V+G ++++
Sbjct: 222 VAPGEDI------LSTVPG-------------GKYATFSGTSMATPHVAGALALIKQLAN 262

Query: 487 SLNPDEI 493
+ ++
Sbjct: 263 ASFERDL 269


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1615DNABINDINGHU922e-28 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 92.1 bits (229), Expect = 2e-28
Identities = 35/88 (39%), Positives = 52/88 (59%)

Query: 2 NKAQLIQRIATSLEQSQASTRPVVEQILQQIHIALSEGEKVFLPQFGTFELRYHLPKSGR 61
NK LI ++A + E ++ + V+ + + L++GEKV L FG FE+R + GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGETMEIAGFNQPSFKAATALKQAI 89
NPQTGE ++I P+FKA ALK A+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1619YERSSTKINASE340.005 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 33.6 bits (76), Expect = 0.005
Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 1/50 (2%)

Query: 182 QVLDGIIHSHANQVLHRDIKPDNILVDD-DGRVHVIDFGISKLMGEQGNG 230
++LD H V+H DIKP N++ D G VID G+ GEQ G
Sbjct: 253 RLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSGEQPKG 302


19Shewana3_1703Shewana3_1741Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_17032210.266893acriflavin resistance protein
Shewana3_1704531-1.077257RND family efflux transporter MFP subunit
Shewana3_1705738-0.422122type II citrate synthase
Shewana3_1706740-0.031118succinate dehydrogenase subunit C
Shewana3_1707743-0.223665succinate dehydrogenase subunit D
Shewana3_1708747-0.266604succinate dehydrogenase flavoprotein subunit
Shewana3_1709744-0.143799succinate dehydrogenase iron-sulfur subunit
Shewana3_1710541-0.1081372-oxoglutarate dehydrogenase E1 component
Shewana3_1711441-1.4335002-oxoglutarate dehydrogenase E2 component
Shewana3_1712130-1.226780succinyl-CoA synthetase subunit beta
Shewana3_1713021-0.523657succinyl-CoA synthetase subunit alpha
Shewana3_17140160.016484GreA/GreB family elongation factor
Shewana3_1715217-1.030913N-acetyltransferase GCN5
Shewana3_1716113-1.543512ferric uptake regulator
Shewana3_1717115-1.310873NAD-dependent deacetylase
Shewana3_1718012-1.422439hypothetical protein
Shewana3_1719012-1.826653hypothetical protein
Shewana3_1720-113-1.881621magnesium and cobalt transport protein CorA
Shewana3_1721-213-2.268220metal dependent phosphohydrolase
Shewana3_1722027-4.659012prolyl 4-hydroxylase subunit alpha
Shewana3_1723-127-4.441379ATPase domain-containing protein
Shewana3_1724-125-3.817428two component transcriptional regulator
Shewana3_1725-126-3.999755hypothetical protein
Shewana3_1726-124-4.026251sodium:dicarboxylate symporter
Shewana3_1727-125-3.922361Ig domain-containing protein
Shewana3_1728014-1.353351succinylarginine dihydrolase
Shewana3_1729013-1.142024DNA topoisomerase I
Shewana3_1730117-2.453515hypothetical protein
Shewana3_1731321-1.438407transcriptional regulator CysB
Shewana3_1732117-0.286438two component LuxR family transcriptional
Shewana3_1733217-0.201722thioesterase superfamily protein
Shewana3_1734115-0.347868phospho-2-dehydro-3-deoxyheptonate aldolase
Shewana3_17351120.394316hypothetical protein
Shewana3_17361130.663764phosphoenolpyruvate synthase
Shewana3_1737-1110.789724FAD linked oxidase domain-containing protein
Shewana3_17381110.330942hypothetical protein
Shewana3_17391110.541057MarR family transcriptional regulator
Shewana3_17401140.402961peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_1741224-0.529947hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1703ACRIFLAVINRP6640.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 664 bits (1715), Expect = 0.0
Identities = 262/1091 (24%), Positives = 470/1091 (43%), Gaps = 94/1091 (8%)

Query: 7 SVKRPVTVWMFMLAIMLFGMVGFSRLAVKLLPDLSYPTLTIRTMYDGAAPVEVEQLVSKP 66
++RP+ W+ + +M+ G + +L V P ++ P +++ Y GA V+ V++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 67 IEEAVGVVKGLRKISSISRS-GMSDVVLEFEWGTTMDMASLDVREKLDTI--ALPLDVKK 123
IE+ + + L +SS S S G + L F+ GT D+A + V+ KL LP +V++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 124 PLLLRFNPNLDPIMRLALSVPNASEAELKQMRTYAEEELKRRLEALSGVAAVRLSGGLEQ 183
+ + +M N + Y +K L L+GV V+L G +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSDNPGT-TQDDISDYVASNVKDTLSRLNGVGDVQLFGA-QY 182

Query: 184 EVHIQLNQEKLSQLNLNADDIKRRINEENINLSAGKVIQGD------REYLVRTLNQFNS 237
+ I L+ + L++ L D+ ++ +N ++AG++ + +F +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKN 242

Query: 238 LEELGQVIVYRDAQ-TLVRLFEVATITDAFKERSDITRIGSQESIELAIYKEGDANTVAV 296
EE G+V + ++ ++VRL +VA + + + I RI + + L I AN +
Sbjct: 243 PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDT 302

Query: 297 AKKLRDELVKINQD-PKQNKLEVIYDQSEFIESAVSEVTSSALMGSILSMLVIYLFLRNI 355
AK ++ +L ++ P+ K+ YD + F++ ++ EV + +L LV+YLFL+N+
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 356 IPTLIISISIPFSVIATFNMMYFADISLNIMSLGGIALAIGLLVDNAIVVLENIDRC-RS 414
TLI +I++P ++ TF ++ S+N +++ G+ LAIGLLVD+AIVV+EN++R
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 415 EGMSKLDAAVTGTKEVAGAIFASTLTTLAVFVPLVFVDGIAGALFSDQALTVTFALLASL 474
+ + +A ++ GA+ + AVF+P+ F G GA++ ++T+ A+ S+
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 475 LVALTSIPMLASREGFTALPELIKKTPKEKPTTKLGKLKHYSATVFSFPIVLLFSYLPSA 534
LVAL P L + L+K E K
Sbjct: 483 LVALILTPALCAT--------LLKPVSAEHHENK-------------------------- 508

Query: 535 LLTLALVIGRFFSWLLGLVMRPLSSGFNFVYHAIESVYHKLLAMALRKQVATLLLTIGIT 594
G FF W FN + + Y + L LL+ I
Sbjct: 509 --------GGFFGW------------FNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIV 548

Query: 595 GACISLLPRLGMELIPPMNQGEFYVEILLPPGTAVGETDKVLQQLAMSI--KDRPEVKHA 652
+ L RL +P +QG F I LP G T KVL Q+ ++ V+
Sbjct: 549 AGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESV 608

Query: 653 YSQAGSGGLMTSDTARGGENWGRLQVVLNDHTAYHQVTQVLRDTARRIPELEAKIEQPEL 712
++ G + +N G V L + R KI +
Sbjct: 609 FTVNGFS------FSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFV 662

Query: 713 FSFKTPLEIEL---TGYDLHLLKRSADNLVKALSASERFA-----------DVNTSLRDG 758
F P +EL TG+D L+ ++ A + V + +
Sbjct: 663 IPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLED 722

Query: 759 QPELSIRFDHARLAALGMDAPTVANRIAQRVGGTVASQYTVRDRKIDILVRSELNERDQI 818
+ + D + ALG+ + I+ +GGT + + R R + V+++ R
Sbjct: 723 TAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLP 782

Query: 819 SDIDALIINPNSPQPIALSAVAEVSLQLGPSAINRISQQRVALVSANLAYG-DLSDAVAE 877
D+D L + + + + SA G + R + + A G DA+A
Sbjct: 783 EDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMAL 842

Query: 878 AQQILSAQVLPASVQARFGGQNEEMEHSFQSLKIALILAVFLVYLVMASQFESLLHPLLI 937
+ + S LPA + + G + + S + ++ +V+L +A+ +ES P+ +
Sbjct: 843 MENLASK--LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 938 LFAVPMALAGSVLGLYITQTHLSVVVFIGLIMLAGIVVNNAIVLVDRINQL-RTEGVDKL 996
+ VP+ + G +L + V +GL+ G+ NAI++V+ L EG +
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 997 EAIKVAAKSRLRPIMMTTLTTTLGLLPMALGLGDGSEVRAPMAITVIFGLSLSTLLTLIV 1056
EA +A + RLRPI+MT+L LG+LP+A+ G GS + + I V+ G+ +TLL +
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1057 IPVLYALFDRK 1067
+PV + + R
Sbjct: 1021 VPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1704RTXTOXIND453e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 45.2 bits (107), Expect = 3e-07
Identities = 38/202 (18%), Positives = 79/202 (39%), Gaps = 24/202 (11%)

Query: 91 LAVIDAKRQ----QYDLDRSEAEVKIIEQELNRLK---KMTNKEFIS--ADSMAKLEYNL 141
AV++ + + +L +++++ IE E+ K ++ + F + D + + N+
Sbjct: 252 HAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNI 311

Query: 142 QAAIARRDLAELQVKESHVVSPIDGIVAKRYVKA-GNMAQEFGDLFYIV-NQDELHGIVH 199
E + + S + +P+ V + V G + L IV D L
Sbjct: 312 GLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTAL 371

Query: 200 LPEQQLTSLRLGQEAQV-FS--NQQSKNAIHAKVLRISP--VVDPQSGT-FKVTLAVP-- 251
+ + + + +GQ A + + KV I+ + D + G F V +++
Sbjct: 372 VQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEEN 431

Query: 252 -----NQNAHLKAGMFTRVELK 268
N+N L +GM E+K
Sbjct: 432 CLSTGNKNIPLSSGMAVTAEIK 453



Score = 40.2 bits (94), Expect = 9e-06
Identities = 12/49 (24%), Positives = 26/49 (53%)

Query: 72 GLIEAINVEEGDRVQKGQILAVIDAKRQQYDLDRSEAEVKIIEQELNRL 120
+++ I V+EG+ V+KG +L + A + D ++++ + E R
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRY 153


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1723PF06580385e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 38.3 bits (89), Expect = 5e-05
Identities = 21/101 (20%), Positives = 34/101 (33%), Gaps = 23/101 (22%)

Query: 356 LMENAFRLCISQ------VQVSARYTDQGDFELIVEDDGPGVEEKLRQKIIQRGVRADTQ 409
L+EN + I+Q + + D G L VE+ G + ++
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTK-DNGTVTLEVENTGSLALKNTKE------------ 309

Query: 410 SPGQGIGLA-VCDEIVSSYGGELSIE-ESHLEGARFRIRIP 448
G GL V + + YG E I+ + IP
Sbjct: 310 --STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1724HTHFIS762e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.4 bits (188), Expect = 2e-18
Identities = 31/120 (25%), Positives = 57/120 (47%), Gaps = 1/120 (0%)

Query: 5 RILVVEDDLILSHHLKVQLSDLGNQVQVALTAKEGFFQATNYPIDVAIVDLGLPDQDGIS 64
ILV +DD + L LS G V++ A + D+ + D+ +PD++
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 65 LIQQLREEGVKAPILILTARVNWQDKVEGLNAGADDYLVKPFQKEELVARLD-ALVRRSA 123
L+ ++++ P+L+++A+ + ++ GA DYL KPF EL+ + AL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1727INTIMIN612e-11 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 61.2 bits (148), Expect = 2e-11
Identities = 50/212 (23%), Positives = 86/212 (40%), Gaps = 24/212 (11%)

Query: 249 VVVKPIDAYSIALTITDSQGQELRNISHSV-PGSVIATLRKDGVPTSYQTISFNLTGQGT 307
+ V + +TD + + + AT++K+GV + +SFN+ GT
Sbjct: 546 ITVLSNGQVVDQVGVTDFTADKTSAKADGTEAITYTATVKKNGVAQANVPVSFNIV-SGT 604

Query: 308 LNPSSGTALTDLNGHASVTLVTGTNAGAGTVTASFSLENETITDSFNFEVAGDAPGGNGE 367
S+ +A T+ +G A+VTL + G V+A + A N
Sbjct: 605 AVLSANSANTNGSGKATVTLKSDK-PGQVVVSA---------------KTAEMTSALNAN 648

Query: 368 ANSLSIQLTNSQTGVPTTDISATQPGKVTVAL---VDKDSTPLVGKVVSFSSTLGNFLPT 424
A Q S T + +A G+ + V K P+ + V+F++TLG +
Sbjct: 649 AVIFVDQTKASITEIKADKTTAVANGQDAITYTVKVMKGDKPVSNQEVTFTTTLGK--LS 706

Query: 425 QGTALTDSLGRASITLTAGSIEGAGEITATYG 456
T TD+ G A +TLT+ + G ++A
Sbjct: 707 NSTEKTDTNGYAKVTLTSTTP-GKSLVSARVS 737



Score = 36.6 bits (84), Expect = 7e-04
Identities = 46/207 (22%), Positives = 81/207 (39%), Gaps = 16/207 (7%)

Query: 32 GTTPQPSVVTVTLSISNSDNVSLDTPAEVQATVVDSKTGPKAGIVVTFKLDNDELGTFTP 91
G VT + S ATV + +A + V+F + + GT
Sbjct: 552 GQVVDQVGVTDFTADKTSAKADGTEAITYTATVKKNGV-AQANVPVSFNIVS---GTAVL 607

Query: 92 STGTQLTDSSGVAKIKLETRNLAGAGSVTASIVTGESASIGFYSKGDGAINPGTGNKLKL 151
S + T+ SG A + L++ +V+ ++A + + I
Sbjct: 608 SANSANTNGSGKATVTLKS------DKPGQVVVSAKTAEMTSALNANAVIFVDQTKASIT 661

Query: 152 FLVNAQGQAITSISTATPGVVKALYTNSSDEPLVGKVITFTSTLGKFQPESGTALTDTQG 211
+ + A+ + A VK + D+P+ + +TFT+TLGK + T TDT G
Sbjct: 662 EIKADKTTAVANGQDAITYTVKVMK---GDKPVSNQEVTFTTTLGK--LSNSTEKTDTNG 716

Query: 212 LAKIAITAGTVAGAGKIIAKADDTESE 238
AK+ +T+ T G + A+ D +
Sbjct: 717 YAKVTLTSTTP-GKSLVSARVSDVAVD 742



Score = 36.2 bits (83), Expect = 0.001
Identities = 64/300 (21%), Positives = 104/300 (34%), Gaps = 38/300 (12%)

Query: 380 TGVPTTDISATQPGKVTV---ALVDKDSTPLVGKVVSFSSTLGNFLPTQGTALTDSLGRA 436
T SA G + A V K+ VSF+ G + + +A T+ G+A
Sbjct: 561 TDFTADKTSAKADGTEAITYTATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKA 620

Query: 437 SITLTAGSIEGAGEITATYGTAKAIIGFVTAGDEIDPVEASPEISFDIYDCNDVATWDKA 496
++TL + G++ + TA + + A+ I D T KA
Sbjct: 621 TVTLKSDKP---GQVVVSAKTA----------EMTSALNANAVIFVD--QTKASITEIKA 665

Query: 497 LKNFEVCKATDNITNDKPGIVGAKVTRSGSTQALQQVLITAATTIGAISPSSGTAITNAE 556
K V D IT KV + + Q+V T TT+G +S S+ T+
Sbjct: 666 DKTTAVANGQDAIT------YTVKVMKGDKPVSNQEV--TFTTTLGKLSNSTEK--TDTN 715

Query: 557 GKAILDLYANGNVGAGEISLKVKDVTATKAFEIGRVNISLKLETSLGGNLLPAGGSTI-- 614
G A + L + G +S +V DV A ++ + ++ + G+ +
Sbjct: 716 GYAKVTLTST-TPGKSLVSARVSDV----AVDVKAPEVEFFTTLTIDDGNIEIVGTGVKG 770

Query: 615 -LDVTVLNPDGS--LATGQPFTLVFTSECQASNKAIIDSPVITNGGKGYATYRSTGCETQ 671
L L A+G + S A S +T KG T + Q
Sbjct: 771 KLPTVWLQYGQVNLKASGGNGKYTWRSANPAIASVDASSGQVTLKEKGTTTISVISSDNQ 830


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1732HTHFIS762e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.0 bits (187), Expect = 2e-18
Identities = 33/110 (30%), Positives = 52/110 (47%), Gaps = 4/110 (3%)

Query: 8 IIIADDHPLFRNALRQALSSAFEHTQWYEADSADALQSVLDSQTVNYDLVLLDLQMPGSH 67
I++ADD R L QALS A + +A L + + DLV+ D+ MP +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVR--ITSNAATLWRWIAAGD--GDLVVTDVVMPDEN 61

Query: 68 GYSTLIHLRSHYPELPVVVISAHEDINTISRAIHYGGSGFIPKSASMETL 117
+ L ++ P+LPV+V+SA T +A G ++PK + L
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTEL 111


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1733TYPE3OMGPROT290.010 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 28.7 bits (64), Expect = 0.010
Identities = 21/97 (21%), Positives = 41/97 (42%), Gaps = 21/97 (21%)

Query: 15 AIEQRINQSEARVIKAVFPSITNHHNTLFGGEALAWMDETAFIAATRFCRKTLVTVSSDR 74
+E + A+V+ P++ N +A+ ET + V V+
Sbjct: 350 LLEN---EGSAQVVSR--PTLLTQENA----QAVIDHSETYY-----------VKVTGKE 389

Query: 75 IDFKKPIPAGTLAELIARVIHVGNTSLKVEVNIFVED 111
+ K I GT+ + RV+ G+ S ++ +N+ +ED
Sbjct: 390 VAELKGITYGTMLRMTPRVLTQGDKS-EISLNLHIED 425


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1736PHPHTRNFRASE3023e-95 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 302 bits (775), Expect = 3e-95
Identities = 110/418 (26%), Positives = 188/418 (44%), Gaps = 65/418 (15%)

Query: 384 QPGDVLVTDMTDPDWEPIMK-RASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIK 442
+ ++ D+T D + K T+ GGRT H+AI++R L +PAVVG +VT++I+
Sbjct: 155 EETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQ 214

Query: 443 NGQLVTVSCAEG---------DTGYIYEGKQEFEVVSNRVDALPALP--------MKIMM 485
+G +V V EG + E + FE L P +++
Sbjct: 215 HGDMVIVDGIEGIVIVNPTEEEVKAYEEKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAA 274

Query: 486 NVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGY 545
N+G P EG+GL R EF+ + +D EE E Y
Sbjct: 275 NIGTPKDVDGVLANGGEGIGLYRTEFL--------------YMDRDQLPTEE--EQFEAY 318

Query: 546 DSPVEFYIARLVEGIASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFR 605
V+ K V++R D ++ + + P+E NP LGFR
Sbjct: 319 KEVVQ---------------RMDGKPVVIRTLDIGGDKELSYL----QLPKELNPFLGFR 359

Query: 606 GASRYISESFRDCFALECEAIKRVRNEMGLKNVEVMIPFVRTVKEAEQVIELLKEQGLER 665
+ + +D F + A+ R N++VM P + T++E Q +++E+ +
Sbjct: 360 AIRLCLEK--QDIFRTQLRALLRAS---TYGNLKVMFPMIATLEELRQAKAIMQEEKDKL 414

Query: 666 GKDG------LRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLSLGLDRDSGIISHL 719
+G + V +M E+PS A+ A+ F + D FSIG+NDL Q ++ DR + +S+L
Sbjct: 415 LSEGVDVSDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYL 474

Query: 720 FDERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTV 777
+ + A+ L+ M IKAA ++G ++G+CG+ D L+ G+D S++ ++
Sbjct: 475 YQPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGDE-VAIPLLLGLGLDEFSMSATSI 531


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1740SUBTILISIN1981e-58 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 198 bits (506), Expect = 1e-58
Identities = 93/279 (33%), Positives = 140/279 (50%), Gaps = 27/279 (9%)

Query: 225 GQGVTVAVLDTGYDLDHNDLAEQVLVSKDFTYSSNG----IDDLNGHGTHTAATIAGTGV 280
G+GV VAVLDTG D DH DL +++ ++FT G D NGHGTH A TIA T
Sbjct: 40 GRGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGDPEIFKDYNGHGTHVAGTIAATEN 99

Query: 281 ESNGRWAGMAPGAKLLVGKVLTNAGSGSTSGILSGMQWAVAQGADVVSMSLGGSGTSCTG 340
E+ G+AP A LL+ KVL GSG I+ G+ +A+ Q D++SMSLGG
Sbjct: 100 ENGV--VGVAPEADLLIIKVLNKQGSGQYDWIIQGIYYAIEQKVDIISMSLGGPE-DVPE 156

Query: 341 PLVDMVEALSDKALFVVSAGNS----FTRETVGIPGCAPSALTVGAVDRDNHTASFSSRG 396
+ +A++ + L + +AGN + +G PGC ++VGA++ D H + FS+
Sbjct: 157 LHEAVKKAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVISVGAINFDRHASEFSNSN 216

Query: 397 PSPDGHSAKPDIASQGVDVVSAASGGFGSTAYRALSGTSMSAPHVSGGAAIVMQARP--- 453
D+ + G D++S GG Y SGTSM+ PHV+G A++ Q
Sbjct: 217 NE-------VDLVAPGEDILSTVPGG----KYATFSGTSMATPHVAGALALIKQLANASF 265

Query: 454 --ELSPRQVKEVLTSSVLPNDSHVLEQGAGPMDVNRAIA 490
+L+ ++ L +P + +G G + +
Sbjct: 266 ERDLTEPELYAQLIKRTIPLGNSPKMEGNGLLYLTAVEE 304


20Shewana3_1789Shewana3_1829Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1789123-5.778388hypothetical protein
Shewana3_1790126-6.620420methyl-accepting chemotaxis sensory transducer
Shewana3_1791233-8.466207*phage integrase family protein
Shewana3_1792236-9.491161hypothetical protein
Shewana3_1793234-8.681318hypothetical protein
Shewana3_1794124-5.495485hypothetical protein
Shewana3_1795319-1.592573hypothetical protein
Shewana3_17964250.614456plasmid stabilization system protein
Shewana3_17974260.745524CopG family transcriptional regulator
Shewana3_17983250.610501retinol acyltransferase domain-containing
Shewana3_17991250.587979phage integrase family protein
Shewana3_1800319-1.469835hypothetical protein
Shewana3_1801324-3.820818hypothetical protein
Shewana3_1802328-4.649943hypothetical protein
Shewana3_1803330-5.509148hypothetical protein
Shewana3_1804234-7.195054DNA repair protein RadC
Shewana3_1805436-8.043824MerR family transcriptional regulator
Shewana3_1806337-7.992764vault protein inter-alpha-trypsin subunit
Shewana3_1807337-8.174014hypothetical protein
Shewana3_1808236-7.819803hypothetical protein
Shewana3_1809236-8.044546hypothetical protein
Shewana3_1810233-7.195553hypothetical protein
Shewana3_1811332-6.839412sigma-54 dependent trancsriptional regulator
Shewana3_1812326-6.345764ATPase
Shewana3_1813116-2.394949von Willebrand factor type A domain-containing
Shewana3_18142151.030910hypothetical protein
Shewana3_18151140.521047hypothetical protein
Shewana3_18161151.201327hypothetical protein
Shewana3_18171151.148172hypothetical protein
Shewana3_18181130.363315hypothetical protein
Shewana3_1819114-1.117500hypothetical protein
Shewana3_1820021-4.229557hypothetical protein
Shewana3_1821121-3.962716hypothetical protein
Shewana3_1822124-5.175745zeta toxin family protein
Shewana3_1823125-4.911553hypothetical protein
Shewana3_1824026-5.022053glutathione synthase
Shewana3_1825128-4.885784ATPase-like protein
Shewana3_1826126-4.381786hypothetical protein
Shewana3_1827025-4.052129hypothetical protein
Shewana3_1828025-4.444540hypothetical protein
Shewana3_1829-123-4.301221hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1790INTIMIN310.017 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 31.2 bits (70), Expect = 0.017
Identities = 38/154 (24%), Positives = 57/154 (37%), Gaps = 15/154 (9%)

Query: 134 VDSLSISVADEVAYYTDLNKQLLAIVDETAKAGANQEIAIKAAAFSAYLQMKERAGIERA 193
V LS S ++ LNK L + E KA Q+I + G A
Sbjct: 73 VADLSKSQDINLSTIWSLNKHLYSSESEMMKAEPGQQIILPLKKLPFEYSALPLLG--SA 130

Query: 194 VLSSTFGQAGFKPKVYAKFITLVSEQNTYLERFLALATPNLVDGIRQLQ----NGNEVKE 249
L + G AG K+ K V++ N ++ L A QLQ NG+ K+
Sbjct: 131 PLVAAGGVAGHTNKL-TKMSPDVTKSNMTDDKALNYAAQQAASLGSQLQSRSLNGDYAKD 189

Query: 250 VEALRQIATDQDNQKIQQQNPEDWFAK-STARID 282
IA +Q + ++Q W TA ++
Sbjct: 190 T--ALGIAGNQASSQLQ-----AWLQHYGTAEVN 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1792RTXTOXIND280.038 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.3 bits (63), Expect = 0.038
Identities = 19/146 (13%), Positives = 42/146 (28%), Gaps = 15/146 (10%)

Query: 68 DPVKADSVL----DTRIRAELLAMDWQ-RALGLAEGKLSVVDINLAPIPLAYGIINANKL 122
+ V+ VL A+ L L + + ++ ++ L +
Sbjct: 116 ESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPY 175

Query: 123 IFSRDEGRRMQEESRIMSQ----------MELDYSAPSHFIILDNAYITSLSLSLSQYQA 172
+ E ++ S I Q EL+ + A I ++
Sbjct: 176 FQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKS 235

Query: 173 ELGELRQLLSQRSLSNLEYRAAERTL 198
L + LL +++++ E
Sbjct: 236 RLDDFSSLLHKQAIAKHAVLEQENKY 261


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1795BONTOXILYSIN290.015 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 29.1 bits (65), Expect = 0.015
Identities = 13/53 (24%), Positives = 26/53 (49%), Gaps = 7/53 (13%)

Query: 60 FPDTFESSDTAVITRFFESLKAQLKADYLAKLRDTSLGQVHESELFYLWVKEI 112
F S D +++ F ++L YL +++ G + + +YLW+KE+
Sbjct: 520 FSKVVSSKDKSLVYSFLDNL-----MSYLETIKND--GPIDTDKKYYLWLKEV 565


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1811HTHFIS320e-107 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 320 bits (821), Expect = e-107
Identities = 121/344 (35%), Positives = 176/344 (51%), Gaps = 28/344 (8%)

Query: 152 FDDIITLDPEMLLLKAKAQVLASHEVSVLICGESGTGKEMFARAIHNASARRDKPFVAIN 211
++ M + L +++++I GESGTGKE+ ARA+H+ RR+ PFVAIN
Sbjct: 136 GMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAIN 195

Query: 212 CGAFPSELIDSILFGHKKGAFTGAVSDKVGVFELAHSGTLFLDEFGELDSSAQVRLLRVL 271
A P +LI+S LFGH+KGAFTGA + G FE A GTLFLDE G++ AQ RLLRVL
Sbjct: 196 MAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVL 255

Query: 272 QDGKFTRLGDSKERSSNFHLITATNRDLMADVSKGRFREDLFYRVAIGVLSLPPLRSRQS 331
Q G++T +G S+ ++ ATN+DL +++G FREDL+YR+ + L LPPLR R
Sbjct: 256 QQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAE 315

Query: 332 DLDHLADQFTAMLTQEYPSLGGKKISTAAKKIISNHRWPGNIRELKATILRAALWSETAV 391
D+ L F +E L K+ A +++ H WPGN+REL+ + R V
Sbjct: 316 DIPDLVRHFVQQAEKEG--LDVKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDV 373

Query: 392 LEEVDIRRAILSTF---------QNSESILERD----------------ISKGVDINSII 426
+ I + S S S+ + + ++
Sbjct: 374 ITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVL 433

Query: 427 DLVERHYLERGLAFTSGNKRKAALLLGYNNHQTLNNRLKKLGLE 470
+E + L T GN+ KAA LLG N TL ++++LG+
Sbjct: 434 AEMEYPLILAALTATRGNQIKAADLLGL-NRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1828RTXTOXIND290.032 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.6 bits (64), Expect = 0.032
Identities = 10/75 (13%), Positives = 29/75 (38%), Gaps = 10/75 (13%)

Query: 106 DLQALKHQLSQAHGANSQLVQQLQQLQ----------SELDEQQNKNLTLENALTSATAK 155
+ + ++++ + +L + EQ+NK + N L ++
Sbjct: 215 ERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQ 274

Query: 156 LKHLEAQYQQAQQAL 170
L+ +E++ A++
Sbjct: 275 LEQIESEILSAKEEY 289


21Shewana3_1839Shewana3_1855Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_18392151.785213peptidase S1 and S6, chymotrypsin/Hap
Shewana3_18424141.787590hypothetical protein
Shewana3_18432172.243866hypothetical protein
Shewana3_18441172.587452hypothetical protein
Shewana3_18451162.855583hypothetical protein
Shewana3_18460192.602957aromatic amino acid transporter
Shewana3_18470193.007006bifunctional phosphoribosyl-AMP
Shewana3_18481183.603879imidazole glycerol phosphate synthase subunit
Shewana3_18491193.0023901-(5-phosphoribosyl)-5-[(5-
Shewana3_18501191.431848imidazole glycerol phosphate synthase subunit
Shewana3_18511190.773336imidazole glycerol-phosphate
Shewana3_1852218-0.042142histidinol-phosphate aminotransferase
Shewana3_1853220-1.758780histidinol dehydrogenase
Shewana3_1854014-2.474203ATP phosphoribosyltransferase
Shewana3_1855017-3.023978hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1839V8PROTEASE406e-06 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 40.0 bits (93), Expect = 6e-06
Identities = 29/186 (15%), Positives = 60/186 (32%), Gaps = 38/186 (20%)

Query: 45 TDGAHGVLIKPDWIVTAAH-ATFCITPGSDIR-----ILDTDHKVDSVFVHKKYQPGISH 98
T A GV++ D ++T H ++ I ++ + +
Sbjct: 101 TFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG 160

Query: 99 DIALIKL------VDPVTDVTPARLYEQSDELGKNIWFIGAGGTGNGLTGQTVDNGANAG 152
D+A++K V PA + + E N G G+
Sbjct: 161 DLAIVKFSPNEQNKHIGEVVKPATM-SNNAETQVNQNITVTGYPGD----------KPVA 209

Query: 153 VLRKAQNSVEFAQGPLLTFTFDSGDEALPLEGVSGGGDSGGP---AYLTLEGIHYLLGIS 209
+ +++ + + +G + + + GG+SG P + GIH+ G+
Sbjct: 210 TMWESKGKITYLKGEAMQYDLS-----------TTGGNSGSPVFNEKNEVIGIHW-GGVP 257

Query: 210 SRVGGG 215
+ G
Sbjct: 258 NEFNGA 263


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1850cdtoxinb310.003 Cytolethal distending toxin B signature.
		>cdtoxinb#Cytolethal distending toxin B signature.

Length = 269

Score = 31.1 bits (70), Expect = 0.003
Identities = 20/65 (30%), Positives = 29/65 (44%), Gaps = 1/65 (1%)

Query: 92 LLSKERGGQALDCQCLGIIPTEIDELDRQILKAEGLPLPHMGWNQLTFSNPSQVHPLFTG 151
L+S E L Q G P+ + I + G+P+ + WN T S P QV+ F+
Sbjct: 48 LISGENAVDILAVQEAGSPPSTAVDTGTLI-PSPGIPVRELIWNLSTNSRPQQVYIYFSA 106

Query: 152 VPAGS 156
V A
Sbjct: 107 VDALG 111


22Shewana3_1898Shewana3_1903Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1898218-5.226220hypothetical protein
Shewana3_1899118-5.531954NapD family protein
Shewana3_1900118-5.659631nitrate reductase catalytic subunit
Shewana3_1901024-8.565646periplasmic nitrate reductase subunit NapB
Shewana3_1902127-8.067544periplasmic nitrate reductase subunit NapC
Shewana3_1903028-7.624266restriction endonuclease
23Shewana3_1967Shewana3_1986Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_19674240.171232ferredoxin
Shewana3_1968423-0.146204ribonucleotide-diphosphate reductase subunit
Shewana3_19692220.010482ribonucleotide-diphosphate reductase subunit
Shewana3_19701170.674188HAD family hydrolase
Shewana3_19711180.0662733-demethylubiquinone-9 3-methyltransferase
Shewana3_1972328-0.735771DNA gyrase subunit A
Shewana3_1973027-1.566252phosphoserine aminotransferase
Shewana3_1974129-1.733548aromatic amino acid aminotransferase
Shewana3_1975127-2.0276893-phosphoshikimate 1-carboxyvinyltransferase
Shewana3_1976432-2.185749cytidylate kinase
Shewana3_1977327-1.48105230S ribosomal protein S1
Shewana3_1978-114-0.377544integration host factor subunit beta
Shewana3_1979-2110.071099hypothetical protein
Shewana3_1980-2110.321490hypothetical protein
Shewana3_1981-1130.455379orotidine 5'-phosphate decarboxylase
Shewana3_1982-1130.010070short chain dehydrogenase
Shewana3_1983-112-0.461642hypothetical protein
Shewana3_19841110.393696acyl-CoA dehydrogenase domain-containing
Shewana3_1985214-0.081012D-alanyl-D-alanine
Shewana3_19862140.314249hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1978DNABINDINGHU1131e-36 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 113 bits (284), Expect = 1e-36
Identities = 31/89 (34%), Positives = 56/89 (62%), Gaps = 1/89 (1%)

Query: 2 TKSELIEKLATRQSQLSAKEVEGAIKEMLEQMATTLESGDRIEIRGFGSFSLHYRAPRTG 61
K +LI K+A ++L+ K+ A+ + +++ L G+++++ GFG+F + RA R G
Sbjct: 3 NKQDLIAKVA-EATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKG 61

Query: 62 RNPKTGSSVELEGKYVPHFKPGKELRERV 90
RNP+TG ++++ VP FK GK L++ V
Sbjct: 62 RNPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1982DHBDHDRGNASE976e-26 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 96.7 bits (240), Expect = 6e-26
Identities = 72/258 (27%), Positives = 119/258 (46%), Gaps = 14/258 (5%)

Query: 10 QGKNVVVVGGTSGINLAIANAFAQAGANVTVASRSQDKIDAAV--LQLKQSNPDGIHLGV 67
+GK + G GI A+A A GA++ + +K++ V L+ + + +
Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPA-- 64

Query: 68 SFDVRDLAAVEQGFEAIASEFGFIDVLVSGAAGNFPATAAKLSANGFKAVMDIDLLGSFQ 127
DVRD AA+++ I E G ID+LV+ A P LS ++A ++ G F
Sbjct: 65 --DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122

Query: 128 VLK-TAYPLLRRPQGNIIQISAPQASIAMPMQAHVCAAKAGVDMLTRTLAVEWGCEGIRI 186
+ + ++ R G+I+ + + A + A ++KA M T+ L +E IR
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 187 NSIIPGPIADTEGFNRLAPSAALQQQVAQS-------VPLKRNGEGQDIANAAMFLGSEY 239
N + PG ++ A +Q + S +PLK+ + DIA+A +FL S
Sbjct: 183 NIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 240 ASYITGVVLPVDGGWSLG 257
A +IT L VDGG +LG
Sbjct: 243 AGHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1985BLACTAMASEA310.009 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 30.9 bits (70), Expect = 0.009
Identities = 34/161 (21%), Positives = 61/161 (37%), Gaps = 22/161 (13%)

Query: 19 IKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHS-QVALLARDLSTNTLLYS 77
I+ ++ L ++ L+ A +QI + S +V ++ DL++ L +
Sbjct: 4 IRLCIISLLATLPLAVHASPQPL--------EQIKLSESQLSGRVGMIEMDLASGRTLTA 55

Query: 78 QQADTLFIPASTQKVLTAVTALATLGPDFRYVTE--------LWSDAPIRQGHIAGSVYL 129
+AD F ST KV+ LA + + L +P+ + H+A + +
Sbjct: 56 WRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTV 115

Query: 130 RFSGDPTLTQDDLKA---LFAHLQKQGITSIEGHLYLIGDK 167
+T D A L A + G + L IGD
Sbjct: 116 GELCAAAITMSDNSAANLLLATV--GGPAGLTAFLRQIGDN 154


24Shewana3_2014Shewana3_2044Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2014-216-4.254578phosphonate ABC transporter periplasmic
Shewana3_2015-122-7.454888putative PAS/PAC sensor protein
Shewana3_2016-132-10.031936two component, sigma54 specific, Fis family
Shewana3_2017138-11.680768methyl-accepting chemotaxis sensory transducer
Shewana3_2018343-13.255613*hypothetical protein
Shewana3_2019344-13.292099hypothetical protein
Shewana3_2020146-13.127348phage integrase family protein
Shewana3_2021031-9.125108metal dependent phosphohydrolase
Shewana3_2022022-6.929590phage integrase family protein
Shewana3_2023-121-6.309252hypothetical protein
Shewana3_2024-121-6.686468hypothetical protein
Shewana3_2025-121-7.231622response regulator receiver protein
Shewana3_2026-119-7.019645type III restriction enzyme, res subunit
Shewana3_2027124-7.640793N-6 DNA methylase
Shewana3_2028232-9.621835restriction modification system DNA specificity
Shewana3_2029332-10.049221hypothetical protein
Shewana3_2030232-9.080568hypothetical protein
Shewana3_2031133-7.802618hypothetical protein
Shewana3_2032233-7.752454hypothetical protein
Shewana3_2033331-7.021078HSR1-like GTP-binding protein
Shewana3_2034128-5.868275hypothetical protein
Shewana3_2035127-5.558607metallo-beta-lactamase superfamily hydrolase
Shewana3_2036126-5.620852hypothetical protein
Shewana3_2037124-5.334561hypothetical protein
Shewana3_2038021-4.438465DEAD/DEAH box helicase
Shewana3_2039015-1.654806**MATE efflux family protein
Shewana3_2040228-3.194720riboflavin synthase subunit alpha
Shewana3_2041233-2.823619hypothetical protein
Shewana3_2043237-2.613627threonyl-tRNA synthetase
Shewana3_2044335-3.450390translation initiation factor IF-3
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2015HTHFIS757e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 74.9 bits (184), Expect = 7e-16
Identities = 34/158 (21%), Positives = 66/158 (41%), Gaps = 1/158 (0%)

Query: 875 GHILLAEDSPANQIIAGTMLTKAGFDITYACNGLEAVKLVAEKPFDLVLMDVRMPEMDGL 934
IL+A+D A + + L++AG+D+ N + +A DLV+ DV MP+ +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 935 EATEVILQNHPEQVILAMSANVMKEEIEHCYRVGMKDFIAKPVKQKTLLHAIQKWLPNAV 994
+ I + P+ +L MSA G D++ KP L+ I + L
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 995 VSLPSSHKPAENTASLL-DEQLLAELEQTLGKASLSNM 1031
+++ L+ + E+ + L + +++
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDL 161


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2016HTHFIS472e-166 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 472 bits (1217), Expect = e-166
Identities = 147/477 (30%), Positives = 252/477 (52%), Gaps = 12/477 (2%)

Query: 4 SALLVEDSMSLGALYTEYLRAEDINVTHVHYGADALKELSNWQPDLLILDIKLPDMSGLD 63
+ L+ +D ++ + + L +V A + ++ DL++ D+ +PD + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 ILKQVQQSHPHISTIMITAHGSIDIAIDAMRSGAFDFLVKPFDAKRLSITVRNALKQKQL 123
+L +++++ P + ++++A + AI A GA+D+L KPFD L + AL + +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 124 LELVTQYENSLPKGHYQGFIGDSLAMQSVYKTIDCVANSKASVFIMGESGTGKEVCAQAI 183
+ +G S AMQ +Y+ + + + ++ I GESGTGKE+ A+A+
Sbjct: 125 R----PSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180

Query: 184 HQVGNRADKPFIALNCASIPKELIESEIFGHCKGAFTGAHNNRDGAATRADGGTLFLDEI 243
H G R + PF+A+N A+IP++LIESE+FGH KGAFTGA G +A+GGTLFLDEI
Sbjct: 181 HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI 240

Query: 244 CEMDLELQSKLLRFIQTGTFQRVGGSKEEHVDIRFISATNREPWEEVKLGHFREDLFYRL 303
+M ++ Q++LLR +Q G + VGG D+R ++ATN++ + + G FREDL+YRL
Sbjct: 241 GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300

Query: 304 HVIPIELPPLRARGNDVLLLAKQLLKTYSKEEGKKFIDFNPQAAAMLQAYDWPGNVRQLQ 363
+V+P+ LPPLR R D+ L + ++ K EG F+ +A +++A+ WPGNVR+L+
Sbjct: 301 NVVPLRLPPLRDRAEDIPDLVRHFVQQAEK-EGLDVKRFDQEALELMKAHPWPGNVRELE 359

Query: 364 NVIRQIVVLNDASHVEPSMFPTPLKPLAQPQTKAQLQTKMQVEATKVDIDTLVQVASREL 423
N++R++ L + + L+ + ++ A + ++ Q +
Sbjct: 360 NLVRRLTALYPQDVITREIIENELRS-------EIPDSPIEKAAARSGSLSISQAVEENM 412

Query: 424 LSESTQEAHTHTADQQRIQPLWLTEKQTIEGAIALCDGNVPKAAALLDISPSTIYRK 480
+ L E I A+ GN KAA LL ++ +T+ +K
Sbjct: 413 RQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKK 469


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2025HTHFIS336e-113 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 336 bits (864), Expect = e-113
Identities = 112/347 (32%), Positives = 184/347 (53%), Gaps = 30/347 (8%)

Query: 178 ITAKSLVMQDTVSKAKRVAASEVPVLILGETGTGKEVMAQAIHRASLRAGQPLKIVNCGA 237
+ +S MQ+ R+ +++ ++I GE+GTGKE++A+A+H R P +N A
Sbjct: 139 LVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAA 198

Query: 238 LAPNLVDSTLFGHKKGAFTGADKDYPGLFEQANNGTLFLDEVGELPLDVQVKLLRALQQG 297
+ +L++S LFGH+KGAFTGA G FEQA GTLFLDE+G++P+D Q +LLR LQQG
Sbjct: 199 IPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQG 258

Query: 298 EVTRLGDTKTINVDVRVIAATHQDLNKLVANGKFREDLFYRLAVGVIRIPSLRERQEDIP 357
E T +G I DVR++AAT++DL + + G FREDL+YRL V +R+P LR+R EDIP
Sbjct: 259 EYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIP 318

Query: 358 LIVGQLVDQINHSASKHPQYISKKISEKGIKFLSSQYWPGNIRDLWSTLNRAFLWSDTSI 417
+V V Q K+ ++ ++ + + WPGN+R+L + + R +
Sbjct: 319 DLVRHFVQQAEKEGLD-----VKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDV 373

Query: 418 MDEYELSQAMLT-----RSQVVEDVPISLTFNDKVD-------------------IVQLT 453
+ + + + + SL+ + V+ ++
Sbjct: 374 ITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVL 433

Query: 454 DNLQKNYVVAALKASGNVKKHATQMLGLKDHQTLTNWMKRLGIATEK 500
++ ++AAL A+ + A +LGL + TL ++ LG++ +
Sbjct: 434 AEMEYPLILAALTATRGNQIKAADLLGL-NRNTLRKKIRELGVSVYR 479


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2033SECA310.015 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 31.0 bits (70), Expect = 0.015
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 6/61 (9%)

Query: 164 RDLNKLSSTVFIINKMD-EVTDLSDPTLFSEQAAIKTETLKEKLQRAASLTDEEVKALRI 222
R L ++ V IIN M+ E+ LSD L KT + +L++ L + +A +
Sbjct: 16 RTLRRMRKVVNIINAMEPEMEKLSDEELKG-----KTAEFRARLEKGEVLENLIPEAFAV 70

Query: 223 V 223
V
Sbjct: 71 V 71


25Shewana3_2090Shewana3_2106Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2090221-1.095335formate dehydrogenase subunit beta
Shewana3_2091427-2.520309formate dehydrogenase subunit gamma
Shewana3_2092630-3.680850formate dehydrogenase accessory protein FdhE
Shewana3_2093833-3.929935transposase
Shewana3_2094732-4.190091hypothetical protein
Shewana3_2095731-4.061490hypothetical protein
Shewana3_2096631-4.197619thioredoxin-disulfide reductase
Shewana3_2097530-4.013682cell division protein MukB
Shewana3_2098429-4.979533hypothetical protein
Shewana3_2099024-5.463201hypothetical protein
Shewana3_2100-217-3.993876transposase IS3/IS911
Shewana3_2101-218-4.153915putative metal dependent phosphohydrolase
Shewana3_2102018-3.528982hypothetical protein
Shewana3_2103120-3.673380restriction endonuclease
Shewana3_2104219-2.887832phage integrase family protein
Shewana3_2105224-2.354042response regulator receiver modulated metal
Shewana3_2106431-2.895246hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2094BACYPHPHTASE330.001 Salmonella/Yersinia modular tyrosine phosphatase si...
		>BACYPHPHTASE#Salmonella/Yersinia modular tyrosine phosphatase

signature.
Length = 468

Score = 32.9 bits (74), Expect = 0.001
Identities = 25/89 (28%), Positives = 41/89 (46%), Gaps = 3/89 (3%)

Query: 97 TPNEYLSLLNKYTNENIDGFDLQGLGQANSIVLSVPDRHVAPIIVRRLLQAAREQRCVDV 156
T + LL N++ +DL+ +G NS+++S+ + + LL+AA Q
Sbjct: 69 TQEDTAKLLQSTVKHNLNNYDLRSVGNGNSVLVSLRSDQMTLQDAKVLLEAALRQESGAR 128

Query: 157 DYVSLEHPSERGRNIAPHTLVFDGFRWHV 185
+VS S AP T V +G R H+
Sbjct: 129 GHVSSHSHSALH---APGTPVREGLRSHL 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2097GPOSANCHOR340.006 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 33.9 bits (77), Expect = 0.006
Identities = 56/290 (19%), Positives = 110/290 (37%), Gaps = 15/290 (5%)

Query: 309 EKSDQDLTECRSKLDTSVENRTSLEQESEQLKPRLASAVEGEIFYKQGKQASVSLLKKEA 368
+ + E K D S+ + S QE E K L A+EG + + A + L+ E
Sbjct: 91 TEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEK 150

Query: 369 Q-LYESLSLSQLADEGADASRNKVEQANQDILDIQAQL-ADVQERFILLEKKAGQYRNAK 426
L + + A EGA + + +A L A E LE
Sbjct: 151 AALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADS 210

Query: 427 SLLDSVKSWCGDSFELAKLKGMIEEYTAQSKQLALEADQLGNKLNSAE-NINEIHAKAAS 485
+ + ++++ E A L + + + K+ + E + A+ A
Sbjct: 211 AKIKTLEA------EKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAE 264

Query: 486 LIRRAGDSIDPNVAKNWFISTELRLEEERPLAVSLEQMRNGLSALKRNHRTVNRMLDRFK 545
L + +++ + A + I T + L + + L N +++ R LD +
Sbjct: 265 LEKALEGAMNFSTADSAKIKTLEAEKAA--LEAEKADLEHQSQVLNANRQSLRRDLDASR 322

Query: 546 QAKLPRMPSNESEYQQLTEDRGEALESAKEVKASVDARYEDERHIQEELK 595
+AK E+E+Q+L E + S + ++ +DA E ++ ++ E +
Sbjct: 323 EAK----KQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQ 368


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2105HTHFIS518e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 51.0 bits (122), Expect = 8e-09
Identities = 22/90 (24%), Positives = 37/90 (41%), Gaps = 9/90 (10%)

Query: 28 KILVVDDEPDVHTVTKLALSRFRLDGRALTFINAYSGEQAKELLAQEQDIAVAFIDVVME 87
ILV DD+ + TV ALSR D R + +A + DVVM
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRI-----TSNAATLWRWIAAGD-GDLVVTDVVMP 58

Query: 88 SDHAGLELVKWIREVQVNKNIRLILRTGQP 117
D +L+ I++ ++ +++ + Q
Sbjct: 59 -DENAFDLLPRIKK--ARPDLPVLVMSAQN 85


26Shewana3_2172Shewana3_2200Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2172-117-3.168047electron transport complex protein RnfG
Shewana3_2173018-3.821082electron transport complex protein RsxE
Shewana3_2174121-5.638332DNA-(apurinic or apyrimidinic site) lyase /
Shewana3_2175224-6.323896phage integrase family protein
Shewana3_2176226-6.797304hypothetical protein
Shewana3_2177228-7.310211hypothetical protein
Shewana3_2178433-8.251132hypothetical protein
Shewana3_2179435-8.342815hypothetical protein
Shewana3_2180434-7.772229hypothetical protein
Shewana3_2181532-7.579898hypothetical protein
Shewana3_2182530-7.194974hypothetical protein
Shewana3_2183531-7.157367hypothetical protein
Shewana3_2184631-7.321808hypothetical protein
Shewana3_2186324-4.683426Type IIA topoisomerase (DNA gyrase/topo II
Shewana3_2187324-4.800344hypothetical protein
Shewana3_2188325-4.676913hypothetical protein
Shewana3_2189022-2.622385hypothetical protein
Shewana3_2190020-1.746045hypothetical protein
Shewana3_2191016-0.571575IS4 family transposase
Shewana3_2192219-3.959938hypothetical protein
Shewana3_2193114-2.345179hypothetical protein
Shewana3_2194216-1.040372hypothetical protein
Shewana3_2195016-2.432033hypothetical protein
Shewana3_2196117-3.194836PepSY-associated TM helix domain-containing
Shewana3_2197119-3.721973beta-lactamase domain-containing protein
Shewana3_2198121-3.960661hypothetical protein
Shewana3_2199222-3.867954hypothetical protein
Shewana3_2200121-3.496900response regulator receiver protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2188GPOSANCHOR421e-05 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 42.0 bits (98), Expect = 1e-05
Identities = 60/379 (15%), Positives = 126/379 (33%), Gaps = 15/379 (3%)

Query: 289 DTLQSARTKYYQLRGSLLDSFNKTVWSIELSKTALTSKQETLKKSVSDLHAELQAFKPEH 348
++T + D F +++L + L+ + LK +L EL K +
Sbjct: 42 AVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKL 101

Query: 349 DRLRSMESEAKSDHKSAVNRLHEIDTAIKIVEECRSKLLPLCPADSRNDQSLLAVLDEQI 408
+ SE S + R +++ A++ + A + ++ A L +
Sbjct: 102 RKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADS----AKIKTLEAEKAALAARK 157

Query: 409 KDCETEIASLLDQAAAIE-RMKNFTSVINRNKEELELRRKALENIDSKLSFLDTITPDAA 467
D E + ++ + A ++K + + KALE + +
Sbjct: 158 ADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLE 217

Query: 468 SILLSINNNFAKLEITPSAEQKTVIQSFAALFASEAEAVYFCGAILPGVNLKRFNNADIK 527
+ ++ A LE A + EAE
Sbjct: 218 AEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFST 277

Query: 528 RELESVIDVLIAKIEHDEKQRATISQNCRLSKERQAEK--LKECTEDLEELRLQKQNLQ- 584
+ + + K + ++ Q+ L+ RQ+ + L E ++L + Q L+
Sbjct: 278 ADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEE 337

Query: 585 GTELLNTQKTKAESDVIRSKE-------EFDKATREFKSASEKFENLSRRYNLANEELTS 637
++ + D+ S+E E K + K + ++L R + + E
Sbjct: 338 QNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQ 397

Query: 638 VGNPLRELNNQISRLEDLE 656
V L E N++++ LE L
Sbjct: 398 VEKALEEANSKLAALEKLN 416


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2200HTHFIS464e-07 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 46.0 bits (109), Expect = 4e-07
Identities = 10/65 (15%), Positives = 24/65 (36%)

Query: 524 SLLLVDDNLFNLEICRSVLESHFTHIHSADRAEEALRLFSTQRPFIVIVDYRLKDTDGLA 583
++L+ DD+ + L + A R + +V+ D + D +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 584 LIKQM 588
L+ ++
Sbjct: 65 LLPRI 69


27Shewana3_2212Shewana3_2217Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2212217-0.235910response regulator receiver modulated
Shewana3_22133170.213174response regulator receiver modulated CheB
Shewana3_22143170.125039chemoreceptor glutamine deamidase CheD
Shewana3_2215215-1.626060chemotaxis protein CheR
Shewana3_2216214-2.042755methyl-accepting chemotaxis sensory transducer
Shewana3_2217014-3.366171CheW protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2212HTHFIS692e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 68.7 bits (168), Expect = 2e-14
Identities = 36/199 (18%), Positives = 70/199 (35%), Gaps = 19/199 (9%)

Query: 255 KILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDLFIFDLYMPDVNGLE 314
IL+ DD ++ + L G V+ + + + + DL + D+ MPD N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 315 LAKMIRQLDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP--SLFVTQVISRAQ 372
L I++ P+LV+S+ +T + + G+ D + K + +
Sbjct: 65 LLPRIKKARPD--LPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 373 RGHDIRSSASRDSLTGLLNHTQILVAARRCYNLAKRINSSVCIAMLDLDHFKQVNDTYGH 432
+ + L+ + + R + + ++ I G
Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMIT--------------GE 168

Query: 433 SGGDKVLLAFAHLLQQSLR 451
SG K L+A A L R
Sbjct: 169 SGTGKELVARA-LHDYGKR 186



Score = 53.7 bits (129), Expect = 1e-09
Identities = 27/123 (21%), Positives = 55/123 (44%), Gaps = 1/123 (0%)

Query: 131 RIAIVEDDSNVGAMITKQLHEFGFNVQHFLNFTDFLGIQNTSPFDLVLLDLILPDYTEAA 190
I + +DD+ + ++ + L G++V+ N DLV+ D+++PD
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 191 LFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVRKIHQWLKMSE 250
L + + + V V+S++ F + A +Y KP + T L+ I + L +
Sbjct: 65 LLPRIKK-ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 251 KQP 253
++P
Sbjct: 124 RRP 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2213HTHFIS665e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 5e-14
Identities = 29/107 (27%), Positives = 49/107 (45%), Gaps = 7/107 (6%)

Query: 3 IKVLVVDDSALIRNLLGKMIE-ADPELSLVGMAADAYMAKDMVNQHRPDVITLDIEMPKV 61
+LV DD A IR +L + + A ++ + AA + D++ D+ MP
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATL---WRWIAAGDGDLVVTDVVMPDE 60

Query: 62 DGLTFLDRLMKARPTAVVMISSLTEEG-ADATFNALGLGAVDFIPKP 107
+ L R+ KARP V++ ++ + A GA D++PKP
Sbjct: 61 NAFDLLPRIKKARPDLPVLV--MSAQNTFMTAIKASEKGAYDYLPKP 105


28Shewana3_2235Shewana3_2241Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2235313-1.788927hypothetical protein
Shewana3_2236215-2.562899lytic transglycosylase, catalytic
Shewana3_2237219-3.066073diguanylate phosphodiesterase
Shewana3_2238221-2.290912hypothetical protein
Shewana3_2239219-0.064343N-acetyltransferase GCN5
Shewana3_22402191.710450hypothetical protein
Shewana3_22412192.465748hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2236PF07520310.015 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 31.1 bits (70), Expect = 0.015
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 7/77 (9%)

Query: 266 KAKRFTPAQDQQLQKYLVRRVLIEQDDNFKD---WADNLLPEMKSDDMFERRLRWAIREQ 322
K + F D + ++R+ ++D N D W + L EM D R +I E+
Sbjct: 175 KPREFRLVSDPGAMSWFLQRLEADEDGNAVDLQLWVSDWLKEMFLDFKRAERPGRSISEE 234

Query: 323 DS----THIARYLDLLS 335
+ H ARYL L
Sbjct: 235 NLPHMFEHWARYLSYLQ 251


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2239SACTRNSFRASE452e-08 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 44.6 bits (105), Expect = 2e-08
Identities = 24/94 (25%), Positives = 42/94 (44%), Gaps = 6/94 (6%)

Query: 41 HMKNGDSVIFLALSEDNEPLGFAQLYPSFSSVAMKRMWYLNDLYVSENARKKGVGRALLQ 100
+++ FL +N +G ++ +++ A + D+ V+++ RKKGVG ALL
Sbjct: 59 YVEEEGKAAFLYYL-ENNCIGRIKIRSNWNGYA-----LIEDIAVAKDYRKKGVGTALLH 112

Query: 101 KVAAFAKNTDAITVKLATAVSNEKAKSLYESEGY 134
K +AK + L T N A Y +
Sbjct: 113 KAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


29Shewana3_2272Shewana3_2279Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_22722171.123352nucleoside diphosphate kinase
Shewana3_22733171.153595hypothetical protein
Shewana3_22742161.37304430S ribosomal protein S6 modification protein
Shewana3_22753180.899039ferredoxin, 2Fe-2S type, ISC system
Shewana3_22762170.689243chaperone protein HscA
Shewana3_22773170.057625co-chaperone HscB
Shewana3_2278219-0.243173iron-sulfur cluster assembly protein IscA
Shewana3_22792200.015857scaffold protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2276SHAPEPROTEIN1058e-27 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 105 bits (263), Expect = 8e-27
Identities = 70/368 (19%), Positives = 139/368 (37%), Gaps = 62/368 (16%)

Query: 22 VGIDLGTTNSLVAAVRSGVTATLPDENGQHSLPSIVRYTQDGIEVGQVAALSSAQDPKNT 81
+ IDLGT N+L+ G+ + PS+V QD +
Sbjct: 13 LSIDLGTANTLIYVKGQGIVL---------NEPSVVAIRQDR------------AGSPKS 51

Query: 82 IVSV----KRFMGRSLTDIQSGEQAFPYQFEASENGLPLFVTP--QGQVNPVQVSAEILR 135
+ +V K+ +GR+ +I + + P G + V+ ++L+
Sbjct: 52 VAAVGHDAKQMLGRTPGNIAA-------------------IRPMKDGVIADFFVTEKMLQ 92

Query: 136 PLVERA-EKTLGGELQGVVITVPAYFDDAQRQGTKDAASLLGVKVLRLLNEPTAAAIAYG 194
+++ + V++ VP +R+ +++A G + + L+ EP AAAI G
Sbjct: 93 HFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAG 152

Query: 195 LDSKQEGVIAIYDLGGGTFDISILRLNRGVFEVLATGGDSALGGDDFDHLLQAHMQQVWQ 254
L + + D+GGGT +++++ LN V +GGD FD + ++++ +
Sbjct: 153 LPVSEATGSMVVDIGGGTTEVAVISLNGVV-----YSSSVRIGGDRFDEAIINYVRRNYG 207

Query: 255 LANIDSQLSRQLLIEARRVKEALTDASEVEASL---TLADGTVLKQLVTKAEFDCLISAL 311
I + ++ E + A E + LA+G + E +
Sbjct: 208 SL-IGEATAERIKHE---IGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEP 263

Query: 312 VKKTIASCRRTLRD-AGVTADEVLE--TVMVGGSTRVPLVREQVEAFFGKAPLTSIDPDR 368
+ +++ L A ++ E V+ GG + + + G + + DP
Sbjct: 264 LTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLT 323

Query: 369 VVAIGAAI 376
VA G
Sbjct: 324 CVARGGGK 331


30Shewana3_2344Shewana3_2356Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2344-123-3.754479arsenical pump membrane protein
Shewana3_2345-229-4.889390arsenate reductase
Shewana3_2346-232-5.452573acriflavin resistance protein
Shewana3_2347-132-6.388317RND family efflux transporter MFP subunit
Shewana3_2348027-5.872525cytochrome c biogenesis protein, transmembrane
Shewana3_2349129-6.030227hypothetical protein
Shewana3_2350131-5.953826redox-active disulfide protein 2
Shewana3_2351130-5.701704ArsR family transcriptional regulator
Shewana3_2352130-5.438994hypothetical protein
Shewana3_2353130-5.272798hypothetical protein
Shewana3_2354031-4.998100phage integrase family protein
Shewana3_2355135-3.742247metal dependent phosphohydrolase
Shewana3_2356131-4.043881phage integrase family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2346ACRIFLAVINRP6190.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 619 bits (1598), Expect = 0.0
Identities = 247/1036 (23%), Positives = 467/1036 (45%), Gaps = 35/1036 (3%)

Query: 2 IIKAAISRRISTAVLAFGLAIFGALNLNMLPVDFLPSVKYPLIKLSIIWQGATPEDIDQN 61
+ I R I VLA L + GAL + LPV P++ P + +S + GA + +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 62 LADPIERELASVDGLDYLSS-SAIEGLYQLDVNYRYGVDVDVAYQDTLAAFNRSTKNLPV 120
+ IE+ + +D L Y+SS S G + + ++ G D D+A +T LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 DIEAPVIIKADPSQLPIVQAVFESESMDLTQ--LRTWIDSWLTQRLLSASGVAAIDVAGG 178
+++ I S ++ A F S++ TQ + ++ S + L +GV + + G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 179 LEREIRIFVDDEKLEAHGLDLTTLERVLSAENLQRVGGRVT------GKYRENIVRVMGE 232
+ +RI++D + L + L + L +N Q G++ G+ +
Sbjct: 181 -QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 233 FSQLAVIQDLVLSRDSSGSIVRIRDVAEVKDSHEDIRMLTRLNGHPAVKVNVIKQADANT 292
F + L +S GS+VR++DVA V+ E+ ++ R+NG PA + + AN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 293 VTTVDNVEDRLADLAPSFPKDIKFTLVENQADYINDSIRGVRNTALEAMALVVLVLFVFL 352
+ T ++ +LA+L P FP+ +K + ++ SI V T EA+ LV LV+++FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 353 GNSRQVLIIAIALPFALLVNFFLMHLAGFSLNIFSLGGLVVAIGVLPDTSIIVVENISRL 412
N R LI IA+P LL F ++ G+S+N ++ G+V+AIG+L D +I+VVEN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVER- 418

Query: 413 RSLHDKANPQSISEEATLEVGGAIMAATVTFIALFVPFLLVPGLITLLFKELVLVILGLM 472
+ DK P+ +E++ ++ GA++ + A+F+P G ++++ + I+ M
Sbjct: 419 VMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAM 478

Query: 473 IIAGLAAITLTPMLGGVLLKANQRE--------FAFSEKINHGLRWAYGALLHSALQFRL 524
++ L A+ LTP L LLK E F + Y + L
Sbjct: 479 ALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTG 538

Query: 525 TTIFIFIGVAIGGVLLFKSAGSEFFPAVDDGRIVVKIRMPAGANLARMDAIAQQVEALVI 584
+ I+ + G V+LF S F P D G + I++PAGA R + QV +
Sbjct: 539 RYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYL 598

Query: 585 GDQR--VRSVFTLSGGAVRGLYTNKIGNEGEVDIELVPSSER---KITTTEYIKELRPKV 639
+++ V SVFT++G + G N G + L P ER + + I + ++
Sbjct: 599 KNEKANVESVFTVNGFSFSGQAQNA----GMAFVSLKPWEERNGDENSAEAVIHRAKMEL 654

Query: 640 AKLLAPGAILAVNQAKMRGIRSVGQAEIE-VEINGSEVDTLFDVANKLAAKLAERP-ELT 697
K + G ++ N + + + + E ++ G D L N+L A+ P L
Sbjct: 655 GK-IRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLV 713

Query: 698 NVYVSLDSSKPEWQVDIDRTLAAEHGLSTKEIAHVLNGYINGSVPTRYREASELYDIRII 757
+V + ++++++D+ A G+S +I ++ + G+ + + + + +
Sbjct: 714 SVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQ 773

Query: 758 MPESQLRSRSDVENISIATPSGHYVRLKDVAKVTAATGPVEIIRKNQIKQVIVRCDPSA- 816
DV+ + + + +G V G + R N + + ++ + +
Sbjct: 774 ADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPG 833

Query: 817 TDLNSAKELVTNILTETTWPTGYTYSIGGKALQMTQMQTTVQSILGYAVFFSFIVLAVQF 876
T A L+ N+ ++ P G Y G + Q +++ + F+ LA +
Sbjct: 834 TSSGDAMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALY 891

Query: 877 NNLRLPLVILFAAPFCLTGIGYGLFFASQPFGATVIIAAMIVLAANVIDGVLLIQTA-ER 935
+ +P+ ++ P + G+ +Q ++ + + + + +L+++ A +
Sbjct: 892 ESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDL 951

Query: 936 QKQQGITLLKATFDAGLSRLRPRLMTVLPAVLGFMPLALAFEEGGELLRPMAAAAIGGLL 995
+++G +++AT A RLRP LMT L +LG +PLA++ G + +GG++
Sbjct: 952 MEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMV 1011

Query: 996 LNVFVALFLVPVLYTF 1011
+A+F VPV +
Sbjct: 1012 SATLLAIFFVPVFFVV 1027


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2347RTXTOXIND310.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.007
Identities = 20/116 (17%), Positives = 42/116 (36%), Gaps = 9/116 (7%)

Query: 70 IVLTGTIEP-TKVASLASPAEGPILNLVVREGDTVNLGQEILRIGRTHAA-------DSL 121
G + + + + ++V+EG++V G +L++ A SL
Sbjct: 84 ATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSL 143

Query: 122 QTSAAEEVRKQVLNLKRIETLVKQHTLPEEQLDEALSSLEKAKAALSQAKQALNDY 177
+ E+ R Q+L + IE ++ S E+ S K+ + +
Sbjct: 144 LQARLEQTRYQIL-SRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTW 198


31Shewana3_2429Shewana3_2434Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2429-125-5.257688NADH:flavin oxidoreductase
Shewana3_2430021-6.306716LysR family transcriptional regulator
Shewana3_2431021-5.863374diguanylate cyclase
Shewana3_2432020-6.077402hypothetical protein
Shewana3_2433-117-5.418234hypothetical protein
Shewana3_2434-113-3.401454hypothetical protein
32Shewana3_2604Shewana3_2640Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2604214-0.236465hypothetical protein
Shewana3_2605217-1.561231patatin
Shewana3_2606213-0.827614DEAD/DEAH box helicase
Shewana3_2607214-0.770489hypothetical protein
Shewana3_2608113-0.454598hypothetical protein
Shewana3_26091130.040821hypothetical protein
Shewana3_26100120.421716peptidase M23B
Shewana3_26112141.286715exonuclease SbcC
Shewana3_2612-1140.295202exodeoxyribonuclease I subunit D
Shewana3_26130150.061962hypothetical protein
Shewana3_2614-212-1.013446LysR family transcriptional regulator
Shewana3_2615-212-2.445054hypothetical protein
Shewana3_2616-116-3.324175RNA-binding S4 domain-containing protein
Shewana3_2617018-3.084705N-acetyltransferase GCN5
Shewana3_2618022-3.538486PpiC-type peptidyl-prolyl cis-trans isomerase
Shewana3_2619022-3.761304hypothetical protein
Shewana3_2620436-4.838546diguanylate cyclase
Shewana3_2621538-4.754925hypothetical protein
Shewana3_2622543-5.870554TonB family protein
Shewana3_2623542-6.010946biopolymer transport protein ExbD/TolR
Shewana3_2624131-4.674584MotA/TolQ/ExbB proton channel
Shewana3_2625123-3.583037MotA/TolQ/ExbB proton channel
Shewana3_2626-120-3.087529hypothetical protein
Shewana3_2627-217-2.862222TonB-dependent receptor
Shewana3_2628-110-0.748455porin
Shewana3_2629-1130.033408DNA polymerase II
Shewana3_2630-114-0.167277ATP-dependent DNA helicase DinG
Shewana3_2631117-4.770925hypothetical protein
Shewana3_2632223-5.841445primosomal replication protein N''
Shewana3_2633329-7.021326hypothetical protein
Shewana3_2634125-4.833114histone deacetylase superfamily protein
Shewana3_2635226-6.920761hypothetical protein
Shewana3_2636329-6.562701hypothetical protein
Shewana3_2637328-5.074050resolvase domain-containing protein
Shewana3_2638122-4.072620KAP P-loop domain-containing protein
Shewana3_2639119-2.427570IS4 family transposase
Shewana3_2640121-3.084219hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2611RTXTOXIND421e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 1e-05
Identities = 35/214 (16%), Positives = 74/214 (34%), Gaps = 25/214 (11%)

Query: 192 DTLKAKAADIRNLVKEQRARRDGILQTAALTSDDELAAEFSRIEPEFAAATAAKEQSVAA 251
DTLK +++ ++ +++ R + L+ EL P+ E+ V
Sbjct: 135 DTLKTQSSLLQARLEQTRYQ--------ILSRSIELNKLPELKLPDEPYFQNVSEEEVLR 186

Query: 252 HLAALKQRDSAQQLFAEFTRLQELQAEALSLNEQQAQIATQTARLDVAKQAIRV------ 305
+ +K+ Q + + + L++++A+ T AR++ + RV
Sbjct: 187 LTSLIKE-----QFSTWQNQKYQKELN---LDKKRAERLTVLARINRYENLSRVEKSRLD 238

Query: 306 --KPLLDNVLSREQEASLAAAQRDSVQSTHDAAKIALAQAETAAQEIIPLEHKLRDLEQQ 363
LL + + + K L Q E+ E++L +
Sbjct: 239 DFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAK-EEYQLVTQLFK 297

Query: 364 HSHLSALVPQLAEFASLEQALAQAKEILQHTKLQ 397
+ L L L LA+ +E Q + ++
Sbjct: 298 NEILDKLRQTTDNIGLLTLELAKNEERQQASVIR 331



Score = 32.1 bits (73), Expect = 0.012
Identities = 26/218 (11%), Positives = 65/218 (29%), Gaps = 20/218 (9%)

Query: 602 AAAQALQQLQEQIKTLQQQESTLTQQLELERERYREQEGKVERLSGQFAEKALRIPEEYR 661
L +L + T + L+ E+ R Q + E L ++
Sbjct: 119 RKGDVLLKL-TALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQ 177

Query: 662 TL--EVLNQAIADNQQQLEQIKRQIDALRAAQQQAAQQSVAAQTALSAAIEHCNGAADLQ 719
+ E + + + ++Q + Q + + + ++ +
Sbjct: 178 NVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYE------NLSR 231

Query: 720 VQAQQ-ALLAALDNAGFTDRDALREALLTDEQMQALAEGIETYHRQCSLNQSQLTQLKTK 778
V+ + ++L + + A+ E + + + Y Q +S++ K +
Sbjct: 232 VEKSRLDDFSSLLHKQAIAKHAVLEQ---ENKYVEAVNELRVYKSQLEQIESEILSAKEE 288

Query: 779 LSESTSPDLDALEALLTERLAQLKTAEEVWSQLNTRLT 816
T + E L +L+ + L L
Sbjct: 289 YQLVT-------QLFKNEILDKLRQTTDNIGLLTLELA 319


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2621SYCDCHAPRONE290.021 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 29.1 bits (65), Expect = 0.021
Identities = 11/51 (21%), Positives = 21/51 (41%)

Query: 198 YFNQKKYKKAVGVLEVMVPLFPDDGRLWVQLAQFYLMVEDYDKSLATYDLA 248
+ KY+ A V + + L D R ++ L + YD ++ +Y
Sbjct: 46 QYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYG 96


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2622PF035441076e-31 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 107 bits (268), Expect = 6e-31
Identities = 37/169 (21%), Positives = 65/169 (38%), Gaps = 10/169 (5%)

Query: 39 TPVIEITMDRQDAKAQNKPRVVPKPPPPPEQPQKPDTTPPDTSSNID----TSMSFNMGG 94
P E + K PKP P P+ P S N
Sbjct: 75 EPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAP 134

Query: 95 VEAGGHSAGGFKLGNMMTRDGDATPIVRIEPQYPIAAARDGKEGWVQLRFTINELGGVDD 154
+A + + + R +PQYP A EG V+++F + G VD+
Sbjct: 135 ARPTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDN 194

Query: 155 VEVIQAEPKRLFDKEAIRALKKWKYKPKIVDGKPLKQPGMTVQLDFTLD 203
V+++ A+P +F++E A+++W+Y+P G+ V + F ++
Sbjct: 195 VQILSAKPANMFEREVKNAMRRWRYEP------GKPGSGIVVNILFKIN 237


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2628ECOLIPORIN823e-19 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 82.3 bits (203), Expect = 3e-19
Identities = 100/419 (23%), Positives = 170/419 (40%), Gaps = 57/419 (13%)

Query: 1 MNKTLVATALAALFLAPTVSAIEIYKDDKNAVEIGGFIDARVINTQGETEVVNG-ASRIN 59
M + ++A + AL A A EIY D N +++ G +D + ++ +G + +
Sbjct: 1 MKRKVLALVIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDDSSK--DGDQTYMR 58

Query: 60 FGFSRE--LTHGWNAFAKLEWGVNPVGSSDIVYNNRFESVQDEFFYNRLGYAGISHDEYG 117
GF E + + + E+ V N E + RL +AG+ +YG
Sbjct: 59 VGFKGETQINDQLTGYGQWEYNVQ---------ANTTEGEGANSW-TRLAFAGLKFGDYG 108

Query: 118 TITIGKQWGAWYDVVYNTNYGFVWDGNAAGVYTFNKDDGAVNGVGRGDKVVQYRNS---- 173
+ G+ +G YDV T+ + G+ ++ D + GR + V YRN+
Sbjct: 109 SFDYGRNYGVLYDVEGWTDMLPEFGGD-----SYTYADNYMT--GRANGVATYRNTDFFG 161

Query: 174 -IGDVSFAVQAQLKNSSFYTCDIENITEEAC---EVEWNAGKKEAQQVTYDYTYGGSVT- 228
+ ++FA+Q Q KN S D+ T ++ ++ G TYD G S
Sbjct: 162 LVDGLNFALQYQGKNESQSADDVNIGTNNRNNGDDIRYDNGDGFGISTTYDIGMGFSAGA 221

Query: 229 -YKAADKLSLMVGINRGEFDIAYGNGDQKTAVDLIYGVGATWGDFDKDGLYVAA------ 281
Y +D+ + V GD+ A + G +D + +Y+A
Sbjct: 222 AYTTSDRTNEQVNAGG-----TIAGGDKADA----WTAGLK---YDANNIYLATMYSETR 269

Query: 282 NVHKEENHDTDNIGRLIKDAYGVETLVSYKFDNGLRPFVSYNILDAGKDYVIQPNFNADP 341
N+ D G + E Y+FD GLRP VS+ ++ GKD + N N D
Sbjct: 270 NMTPYGKTDKGYDGGVANKTQNFEVTAQYQFDFGLRPAVSF-LMSKGKD-LTYNNVNGDD 327

Query: 342 NDVFKRQFVVVGLHFVWDPNTVLYVEARKDYSDFTSSDQAQEARMSLSEDDGIAIGIRY 400
D+ K + VG + ++ N YV+ + + D D +S DD +A+G+ Y
Sbjct: 328 KDLVK--YADVGATYYFNKNFSTYVDYKINLLD---DDDPFYKDAGISTDDIVALGMVY 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2633PF06776280.021 Invasion associated locus B
		>PF06776#Invasion associated locus B

Length = 214

Score = 28.4 bits (63), Expect = 0.021
Identities = 12/78 (15%), Positives = 20/78 (25%), Gaps = 12/78 (15%)

Query: 159 AAAATGAAGTAAVVAAPAPTSAQAAVNATSLTAP-------VDTSKAAGANPQQ--MLQY 209
A GA A A A + + + GA +Q ++Q
Sbjct: 44 LARRNGARLMLAGAMAIALSFGWSDRADAQGAVRSVHGDWQIRCDTPPGAKAEQCALIQ- 102

Query: 210 WWLQADEKTRKEFMSWAI 227
E ++ I
Sbjct: 103 --SVVAEDRSNAGLTVII 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2636BACTRLTOXIN290.048 Bacterial toxin signature.
		>BACTRLTOXIN#Bacterial toxin signature.

Length = 266

Score = 28.7 bits (64), Expect = 0.048
Identities = 10/44 (22%), Positives = 19/44 (43%)

Query: 346 FLNKFLKTKFKMTDEQIRKAFYHYLCYQSAMRISLRTSSKKAPI 389
LN+ L K+K + + Y+ CY S+ + + K +
Sbjct: 95 LLNEDLAKKYKDEVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCM 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2638VACJLIPOPROT300.014 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 30.3 bits (68), Expect = 0.014
Identities = 22/122 (18%), Positives = 40/122 (32%), Gaps = 23/122 (18%)

Query: 242 FDRKVKLPQVSVLDYLIARQLDFDKYKSDYIHLFPFTKNYKKNISVFA----ALFESNRI 297
F+R + +VLD I R P ++ + A + F N
Sbjct: 35 FNRTMYNFNFNVLDPYIVR---------------PVAVAWRDYVPQPARNGLSNFTGN-- 77

Query: 298 ELRGVEQILNRFFASLEYVASTRMSSDVAINTIVLMVGLIEQHLEMSELVRRQNSSDLSF 357
L ++N F + + +NTI+ M G I+ + ++R
Sbjct: 78 -LEEPAVMVNYF-LQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGS 135

Query: 358 SL 359
+L
Sbjct: 136 TL 137


33Shewana3_2654Shewana3_2660Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2654221-0.641800ABC transporter-like protein
Shewana3_2655323-0.765877trans-2-enoyl-CoA reductase
Shewana3_2656323-1.035963molybdenum-pterin binding
Shewana3_2657529-1.308809PpiC-type peptidyl-prolyl cis-trans isomerase
Shewana3_2658427-1.214700histone family protein DNA-binding protein
Shewana3_2659325-0.959296Lon-A peptidase
Shewana3_2660331-1.726431ATP-dependent protease ATP-binding subunit ClpX
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2654HTHFIS290.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.020
Identities = 13/28 (46%), Positives = 16/28 (57%)

Query: 42 TLAIVGEAGSGKSTLARILVGAEPRSGG 69
TL I GE+G+GK +AR L R G
Sbjct: 162 TLMITGESGTGKELVARALHDYGKRRNG 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2657SHAPEPROTEIN300.025 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 30.1 bits (68), Expect = 0.025
Identities = 50/203 (24%), Positives = 83/203 (40%), Gaps = 37/203 (18%)

Query: 428 MFSRD---DVPTA------------LNKPDVV-----KAAFSDTVLRQGLNSE-VIELEP 466
MFS D D+ TA LN+P VV +A +V G +++ ++ P
Sbjct: 8 MFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAAVGHDAKQMLGRTP 67

Query: 467 NHVVVIR-MKEHHDAGTMPLAEVKADIAERLKQDQANEAARAKAQELMTQVKAGATDVSL 525
++ IR MK+ G + V + + + + + + ++ V GAT V
Sbjct: 68 GNIAAIRPMKD----GVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVER 123

Query: 526 TA--KTKLGRGAQDVD------AAIVGKAFQMPTPTATPVVDTVGLANGYAVIALDKVNA 577
A ++ G GA++V AA +G + T + VVD G AVI+L+ V
Sbjct: 124 RAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVY 183

Query: 578 AESV---SDELVNALKQRLNAQY 597
+ SV D A+ + Y
Sbjct: 184 SSSVRIGGDRFDEAIINYVRRNY 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2658DNABINDINGHU1188e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 118 bits (297), Expect = 8e-39
Identities = 52/88 (59%), Positives = 69/88 (78%)

Query: 2 NKSELIEKIASGADISKAAAGRALDSFIAAVTEGLKEGDKISLVGFGTFEVRERAERTGR 61
NK +LI K+A +++K + A+D+ +AV+ L +G+K+ L+GFG FEVRERA R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGQEIKIAAAKIPAFKAGKALKDAV 89
NPQTG+EIKI A+K+PAFKAGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2659PF05272330.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 32.7 bits (74), Expect = 0.006
Identities = 15/86 (17%), Positives = 35/86 (40%), Gaps = 6/86 (6%)

Query: 286 AEATVVRSYVDWMTSVPWSQRSKIKRDLAKAQEVLDTDHYGLEKVKDRILEYLAVQSRVR 345
A+ V + DW+ + W + ++++ L D+ +++ + V
Sbjct: 527 ADMNRVHPFRDWVKAQQWDEVPRLEKWLVHVLGKTPDDYKPRRLRYLQLVGKYILMGHVA 586

Query: 346 QLKGP------ILCLVGPPGVGKTSL 365
++ P + L G G+GK++L
Sbjct: 587 RVMEPGCKFDYSVVLEGTGGIGKSTL 612


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2660HTHFIS300.018 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.2 bits (68), Expect = 0.018
Identities = 14/70 (20%), Positives = 28/70 (40%), Gaps = 13/70 (18%)

Query: 64 KLPTPHELRAHLDDYVIGQDRAKKVLSVAVYNHYKRLRNSSPKDGVELGKSNILLIGPTG 123
+ P+ E + ++G+ A +Y RL + +++ G +G
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAA----MQEIYRVLARLMQT---------DLTLMITGESG 170

Query: 124 SGKTLLAETL 133
+GK L+A L
Sbjct: 171 TGKELVARAL 180


34Shewana3_2670Shewana3_2676Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2670215-0.051629ferrous iron transport protein B
Shewana3_2671420-0.139082FeoA family protein
Shewana3_2672420-0.013821cytochrome C family protein
Shewana3_2673521-0.066004outer membrane protein
Shewana3_26746220.082407decaheme cytochrome c MtrF
Shewana3_26756240.015403decaheme cytochrome c
Shewana3_26764160.424080decaheme cytochrome c
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2670TCRTETOQM396e-05 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 39.1 bits (91), Expect = 6e-05
Identities = 37/148 (25%), Positives = 58/148 (39%), Gaps = 47/148 (31%)

Query: 14 NAGKSTLFNAL---TGANQQVG---------NW------SGVTVEKKTGHFTLNGADVYL 55
+AGK+TL +L +GA ++G + G+T++ F V +
Sbjct: 13 DAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNI 72

Query: 56 TDLPGIYDLLPAGNSCDCSLDEQIAQQYLAEQRVDGIINLVDA-------TNIERHLYLT 108
D PG D +A+ Y + +DG I L+ A T I L
Sbjct: 73 IDTPGHMDF--------------LAEVYRSLSVLDGAILLISAKDGVQAQTRI-----LF 113

Query: 109 AQLRELSIPMVVVLNKIDAAIKRGIRVD 136
LR++ IP + +NKID GI +
Sbjct: 114 HALRKMGIPTIFFINKIDQN---GIDLS 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2674INTIMIN320.008 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 32.0 bits (72), Expect = 0.008
Identities = 23/114 (20%), Positives = 40/114 (35%), Gaps = 13/114 (11%)

Query: 325 PSISSASMDANGTVTVAVTLSNPATGTV--------YSESADKLKFISDLRVYAN----W 372
S S+ D NG V +T + P V A +++F + L +
Sbjct: 705 LSNSTEKTDTNGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFFTTLTIDDGNIEIV 764

Query: 373 GTSFDYSTRSARSIRLPESTPVSGSNGTYTYTISGLTVPAGTEADHGGLAIQGR 426
GT + + SG NG YT+ + A +A G + ++ +
Sbjct: 765 GTGVKGKLPTVWLQYGQVNLKASGGNGKYTWRSAN-PAIASVDASSGQVTLKEK 817



Score = 30.4 bits (68), Expect = 0.024
Identities = 22/81 (27%), Positives = 34/81 (41%), Gaps = 5/81 (6%)

Query: 325 PSISSASMDANGTVTVAVTLSNPATGTVYSESADKLKFISDLRVYANWGTSFDYSTRSAR 384
S +SA+ + +G TV + P V SA + S L AN D + S
Sbjct: 607 LSANSANTNGSGKATVTLKSDKPGQVVV---SAKTAEMTSALN--ANAVIFVDQTKASIT 661

Query: 385 SIRLPESTPVSGSNGTYTYTI 405
I+ ++T V+ TYT+
Sbjct: 662 EIKADKTTAVANGQDAITYTV 682


35Shewana3_2687Shewana3_2697Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2687219-1.332316NAD-dependent DNA ligase
Shewana3_26884201.606456hypothetical protein
Shewana3_26893192.474486hypothetical protein
Shewana3_26902192.454828short chain dehydrogenase
Shewana3_26912181.798122DNA-O6-methylguanine--protein-cysteine
Shewana3_26922172.458398streptogramin A acetyl transferase
Shewana3_26931172.961155AraC family transcriptional regulator
Shewana3_26940172.615440AzlC family protein
Shewana3_26950172.752631branched-chain amino acid transport
Shewana3_26961133.480872major facilitator superfamily transporter
Shewana3_2697-1133.560603N-acetylglucosamine 6-phosphate deacetylase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2690DHBDHDRGNASE1161e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 116 bits (292), Expect = 1e-33
Identities = 83/259 (32%), Positives = 119/259 (45%), Gaps = 15/259 (5%)

Query: 4 LQGKVAIITGASSGIGYATAKRFAREGAKLVLGARRGAILASLVDEIITQGGEAIYLAGD 63
++GK+A ITGA+ GIG A A+ A +GA + L +V + + A D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 64 VTDEVYASDLVALAVEQYGGLDIAFNNVGINGELGVDSDALSRAEWENTLTTNLTSAFLA 123
V D ++ A + G +DI N G+ + S LS EWE T + N T F A
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHS--LSDEEWEATFSVNSTGVFNA 123

Query: 124 AKYQLPQMLKRGAGSIIFTSSFVGYTIGFPQT--AAYAASKAGMIGLTQSLAVEYGARGI 181
++ M+ R +GSI+ S P+T AAYA+SKA + T+ L +E I
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGV---PRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 182 RVNALLPGGTDTPMGREFANTPEAMAFV--------KNLHALKRLADPAEIAQSALYLAS 233
R N + PG T+T M V K LK+LA P++IA + L+L S
Sbjct: 181 RCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240

Query: 234 DAASFTTGIALLVDGGVSI 252
A T L VDGG ++
Sbjct: 241 GQAGHITMHNLCVDGGATL 259


36Shewana3_2720Shewana3_2727Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2720-116-3.721116hypothetical protein
Shewana3_2721021-6.338488glyoxalase/bleomycin resistance
Shewana3_2722124-7.418532LysR family transcriptional regulator
Shewana3_2723232-9.415815hypothetical protein
Shewana3_2724437-10.683331N-acetyltransferase GCN5
Shewana3_2725336-10.529839hypothetical protein
Shewana3_2726229-8.407567hypothetical protein
Shewana3_2727-119-4.815425hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2724SACTRNSFRASE472e-09 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 46.9 bits (111), Expect = 2e-09
Identities = 19/85 (22%), Positives = 41/85 (48%), Gaps = 1/85 (1%)

Query: 39 YLKRNQGLSFVAVSDSLVIGAVLVGTD-GRRGYVQHLAVSSDFRGQGIGKSLIQKATDAL 97
Y++ +F+ ++ IG + + ++ ++ +AV+ D+R +G+G +L+ KA +
Sbjct: 59 YVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWA 118

Query: 98 SRIGISKTHLFVLIENVTAQDFYTK 122
L N++A FY K
Sbjct: 119 KENHFCGLMLETQDINISACHFYAK 143


37Shewana3_2739Shewana3_2754Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2739-117-3.109747hypothetical protein
Shewana3_2740015-0.227792hypothetical protein
Shewana3_27410140.037025hypothetical protein
Shewana3_27420131.082529lysine exporter protein LysE/YggA
Shewana3_27430171.910125hypothetical protein
Shewana3_27440153.222909hypothetical protein
Shewana3_27450164.218697peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_27460224.725450hypothetical protein
Shewana3_27471234.847064acetyl-CoA hydrolase/transferase
Shewana3_27480214.601781ABC transporter
Shewana3_27491195.163232ABC transporter-like protein
Shewana3_27503162.379227secretion protein HlyD family protein
Shewana3_27512161.164602TetR family transcriptional regulator
Shewana3_27521170.978594hypothetical protein
Shewana3_27531160.189676hypothetical protein
Shewana3_27542160.239608hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2745SUBTILISIN1437e-41 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 143 bits (363), Expect = 7e-41
Identities = 70/210 (33%), Positives = 97/210 (46%), Gaps = 24/210 (11%)

Query: 126 AGMKVCIIDSGLDSSNPDFNWNNITG----DNDSGTGNWYQNGGPHGTHVAGTIGAADNN 181
G+KV ++D+G D+ +PD I G D+D G +++ HGTHVAGTI A +N
Sbjct: 41 RGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGDPEIFKDYNGHGTHVAGTIAATENE 100

Query: 182 IGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKNA 241
GVVG+AP + I+KV N G G + IISMSLGG A
Sbjct: 101 NGVVGVAPEADLLIIKVLNKQGSGQYDWIIQGIYYAIEQKVDIISMSLGGPEDVPELHEA 160

Query: 242 FDAFTAAGGLVVAAAGNDGNSVRS-----YPAGYPSVMMIGANDANNKIADFSQYPSCVS 296
A+ LV+ AAGN+G+ YP Y V+ +GA + + ++FS
Sbjct: 161 VKKAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVISVGAINFDRHASEFSNS----- 215

Query: 297 GRGKKAVNDDGICVEVTAGGVDTLSTYPAG 326
V++ A G D LST P G
Sbjct: 216 ----------NNEVDLVAPGEDILSTVPGG 235



Score = 53.3 bits (128), Expect = 9e-10
Identities = 19/70 (27%), Positives = 27/70 (38%), Gaps = 7/70 (10%)

Query: 447 YGFMSGTSMATPAVSGMAALVWSN-----HSQCTGTQIRNALKATAMDAGTVGKDNYFGY 501
Y SGTSMATP V+G AL+ T ++ L + G G
Sbjct: 237 YATFSGTSMATPHVAGALALIKQLANASFERDLTEPELYAQLIKRTIPLG--NSPKMEGN 294

Query: 502 GIVNAKAADA 511
G++ A +
Sbjct: 295 GLLYLTAVEE 304


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2748ABC2TRNSPORT382e-05 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 38.4 bits (89), Expect = 2e-05
Identities = 44/166 (26%), Positives = 79/166 (47%), Gaps = 23/166 (13%)

Query: 186 GVILTMTMVMFT----SAAIVREREQGNMEFLITTPVRPLELMLGKI----TPYVLVGFV 237
G++ T M T AA R Q E ++ T +R +++LG++ T L G
Sbjct: 72 GMVATSAMTAATFETIYAAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAG 131

Query: 238 QLAIILSAGH-----LLFAVPIRGGLDSIALAAMLFICASLTLGLVISTIAKTQLQSMQM 292
+ + G+ LL+A+P+ IAL + F +LG+V++ +A + +
Sbjct: 132 IGVVAAALGYTQWLSLLYALPV------IALTGLAFA----SLGMVVTALAPSYDYFIFY 181

Query: 293 TVFILLPSILLSGFMFPYEAMPVAAQWIAEALPATHFMRMSRAIVL 338
++ P + LSG +FP + +P+ Q A LP +H + + R I+L
Sbjct: 182 QTLVITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIML 227


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2750RTXTOXIND522e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.8 bits (124), Expect = 2e-09
Identities = 30/144 (20%), Positives = 50/144 (34%), Gaps = 9/144 (6%)

Query: 51 TVERDRLTLTAPVGELINQINVVEGQQVQAGEVLLELDSTAAKARLGQRQAELKQA---- 106
T + ++ +I V EG+ V+ G+VLL+L + A+A + Q+ L QA
Sbjct: 91 THSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ 150

Query: 107 ---QAKLEEAVTGARSEDIDKARAALDGANASVKEVQQNFERTQR--LFKTKVLSQADLD 161
Q E + + + Q K + +LD
Sbjct: 151 TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLD 210

Query: 162 AALAARDTSLAKQAEAEQSLRLLQ 185
A R T LA+ E R+ +
Sbjct: 211 KKRAERLTVLARINRYENLSRVEK 234



Score = 49.4 bits (118), Expect = 8e-09
Identities = 32/239 (13%), Positives = 73/239 (30%), Gaps = 30/239 (12%)

Query: 76 QQVQAGEVLLELDSTAAKARLGQRQAELKQAQAKLEEAVTGARSEDIDKARAALDGANAS 135
Q V EVL K + Q + Q + L+ + + A ++
Sbjct: 177 QNVSEEEVLRLTS--LIKEQFSTWQNQKYQKELNLD-----KKRAERLTVLARINRYENL 229

Query: 136 VKEVQQNFERTQRLFKTKVLS--------------QADLDAALAARDTSLAKQAEAEQSL 181
+ + + L + ++ +L + + ++ A++
Sbjct: 230 SRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEY 289

Query: 182 RLLQNGTRSEQLEQARAAVEAAMAGVAQEQKALKDLSLVAARS----AVVDTLPWRVGDR 237
+L+ ++E L++ R + + K + R+ V G
Sbjct: 290 QLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGV 349

Query: 238 VAAGSQLIGLLAIEHPY-VRVYLPATWLDRVKAGSQVKILVDG----RAQPIAGTVRNI 291
V L+ ++ + V + + + G I V+ R + G V+NI
Sbjct: 350 VTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNI 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2751HTHTETR685e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 67.7 bits (165), Expect = 5e-16
Identities = 26/155 (16%), Positives = 56/155 (36%), Gaps = 6/155 (3%)

Query: 31 SDARQRLIAAAVTLFSERSYPTVSTREIAREAEVDAALIRYYFDSKAGLFEQMVRETLEP 90
+ RQ ++ A+ LFS++ + S EIA+ A V I ++F K+ LF ++ +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 91 VIARLREISSAQAPND---IGELMQTYYRVMAPNLGLPRLIVRVLQEGDGTEAYRIMLSV 147
+ E + + + E++ L+ + + + ++
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 148 FEQILSLSRQWVESAL---VNAGLLKEGLDPNLVR 179
+ S +E L + A +L L
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAA 164


38Shewana3_2836Shewana3_2841Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_28362142.907568SpoOM family protein
Shewana3_28372143.123293putative lipoprotein
Shewana3_28382143.2321094'-phosphopantetheinyl transferase
Shewana3_28392153.142529transcriptional regulator
Shewana3_28402152.993571beta-ketoacyl synthase
Shewana3_28412142.481416omega-3 polyunsaturated fatty acid synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2837TYPE4SSCAGA270.046 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 27.0 bits (59), Expect = 0.046
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 2/66 (3%)

Query: 91 AKVAELLGDLQKVMEQEVSFDQVASLLQKGADAAAYAKSLIEQQGVEQAMQSLKQMALAS 150
+KV + DL+ ++ + +V + A + AK+ + VEQA+ LK +
Sbjct: 758 SKVTQAKSDLENSVKDVIINQKVTDKVDNLNQAVSVAKATGDFSRVEQALADLKN--FSK 815

Query: 151 EQFAQQ 156
EQ AQQ
Sbjct: 816 EQLAQQ 821


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2840PF03544340.005 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 34.2 bits (78), Expect = 0.005
Identities = 25/125 (20%), Positives = 38/125 (30%), Gaps = 10/125 (8%)

Query: 1128 QLQTSSSQAALALLGQKPAPQVQAPIQTAAPVA----VAVATPVAPAQAPVVQALAAEPK 1183
+L + ++ ++ QA PV P P +APVV +PK
Sbjct: 42 ELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIE-KPKPK 100

Query: 1184 ATVVPVSEPKVQQPQVTQQVAQPQVQTVAAATRALSEKPVVQQIETAMMAVVADKTGYPV 1243
P KV+QP+ + A+ + P TA A T
Sbjct: 101 PKPKPKPVKKVEQPK-----RDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVAS 155

Query: 1244 EMLEL 1248
L
Sbjct: 156 GPRAL 160


39Shewana3_2908Shewana3_2921Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2908116-3.084179hypothetical protein
Shewana3_2909015-3.318324hypothetical protein
Shewana3_2910-111-1.127377hypothetical protein
Shewana3_2912014-1.040381secretion protein HlyD family protein
Shewana3_2913016-1.651259hypothetical protein
Shewana3_2914318-2.490961Ion transport 2 domain-containing protein
Shewana3_2915320-3.206014phage integrase family protein
Shewana3_2916320-3.622949hypothetical protein
Shewana3_2917017-3.499362hypothetical protein
Shewana3_2918-114-2.864957hypothetical protein
Shewana3_2919-211-2.219276DNA helicase/exodeoxyribonuclease V subunit
Shewana3_2920112-2.158316hypothetical protein
Shewana3_2921215-0.637200hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2912RTXTOXIND582e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 58.3 bits (141), Expect = 2e-11
Identities = 32/203 (15%), Positives = 73/203 (35%), Gaps = 8/203 (3%)

Query: 87 IALAKAKSDYETVLNSVKANNEGVKAAEAKLQAMRASYNNSVKDAER---QERLYRQDPG 143
+ L K +++ TVL + + +++L + + QE Y +
Sbjct: 207 LNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVN 266

Query: 144 AISVRRLEIAQASRETASSQVTAAEADVRRAIEAAGVNGENNSQLLSARSAVNKAERDRQ 203
+ V + ++ Q E S++ E + + + K E +Q
Sbjct: 267 ELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQ 326

Query: 204 NTHVVAPSRGVITDLNT-DVGQFINAGAPAMTLIAIHDV-WISADLTENNLGNIKVGNRV 261
+ + AP + L G + M ++ D ++A + ++G I VG
Sbjct: 327 ASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNA 386

Query: 262 SILLDSMPGQLF---SGQIRSIG 281
I +++ P + G++++I
Sbjct: 387 IIKVEAFPYTRYGYLVGKVKNIN 409


40Shewana3_2946Shewana3_2962Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2946-115-3.079975diguanylate cyclase
Shewana3_2947117-2.259074hypothetical protein
Shewana3_2948-117-2.103847uroporphyrin-III C/tetrapyrrole
Shewana3_2949-119-4.702108SmpA/OmlA domain-containing protein
Shewana3_2950-124-5.396708hypothetical protein
Shewana3_2951025-5.616880cyclase/dehydrase
Shewana3_2952029-5.803482SsrA-binding protein
Shewana3_2953131-6.170545phage integrase family protein
Shewana3_2954228-6.390260hypothetical protein
Shewana3_2955225-5.247979hypothetical protein
Shewana3_2956119-3.851410hypothetical protein
Shewana3_2957018-3.648867HNH endonuclease
Shewana3_2958-115-3.443035hypothetical protein
Shewana3_2959-116-3.845168hypothetical protein
Shewana3_2960-214-3.279562restriction modification system DNA specificity
Shewana3_2961-211-2.822084N-6 DNA methylase
Shewana3_2962-114-3.333440filamentation induced by cAMP protein fic
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2962FLGFLIH310.006 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 30.9 bits (69), Expect = 0.006
Identities = 38/145 (26%), Positives = 63/145 (43%), Gaps = 16/145 (11%)

Query: 218 IEQQLLTLPILYLSRYIVQHKADYYRLLNQVTREGDWQSWLLFMLKGVEQMASWTCGKIA 277
+EQQL L + H+ Y + + ++G Q + + +G+EQ + + A
Sbjct: 40 LEQQLAQLQMQ-------AHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQA 92

Query: 278 AVRELMEQ-TSEYVRT--ALPKIYSHELVQVIFEQPYCRIGNLVERDIAKRQTASVYLKQ 334
+ M+Q SE+ T AL + + L+Q+ E IG D S +KQ
Sbjct: 93 PIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVD------NSALIKQ 146

Query: 335 LADIGVLEELTIGKEKLFVHPKLMQ 359
+ + E L GK +L VHP +Q
Sbjct: 147 IQQLLQQEPLFSGKPQLRVHPDDLQ 171


41Shewana3_3013Shewana3_3025Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_30132190.048619phospho-2-dehydro-3-deoxyheptonate aldolase
Shewana3_3014117-0.12556950S ribosomal protein L19
Shewana3_3015011-0.220145tRNA (guanine-N(1)-)-methyltransferase
Shewana3_3016-113-1.04774816S rRNA-processing protein RimM
Shewana3_3017012-0.46894130S ribosomal protein S16
Shewana3_3018112-0.054006signal recognition particle protein
Shewana3_3019213-0.559030cytochrome c assembly protein
Shewana3_30203120.146822hypothetical protein
Shewana3_30213130.272819hypothetical protein
Shewana3_30222130.8770254'-phosphopantetheinyl transferase
Shewana3_3023217-0.000610pyridoxine 5'-phosphate synthase
Shewana3_3024216-0.329050DNA repair protein RecO
Shewana3_3025218-0.856607GTP-binding protein Era
42Shewana3_3117Shewana3_3146Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3117019-3.881903hypothetical protein
Shewana3_3118124-4.708597hypothetical protein
Shewana3_3119126-5.115986hypothetical protein
Shewana3_3120228-5.106827tryptophan halogenase
Shewana3_3121231-5.502460TonB-dependent receptor
Shewana3_3122-128-6.595232LacI family transcriptional regulator
Shewana3_3123030-6.392106FAD-dependent pyridine nucleotide-disulfide
Shewana3_3124545-8.685805nitrogen regulatory protein P-II
Shewana3_3125546-8.751552methylation site containing protein
Shewana3_3126546-8.551825methylation site containing protein
Shewana3_3127546-8.369194hypothetical protein
Shewana3_3128442-7.245171methylation site containing protein
Shewana3_3129340-6.999596type IV pilin biogenesis protein
Shewana3_3130027-4.496730type IV pilus assembly protein PilX
Shewana3_3131019-2.413156prepilin-type cleavage/methylation-like protein
Shewana3_3132118-1.517966type IV pilus modification protein PilV
Shewana3_3133016-1.4827694-hydroxy-3-methylbut-2-enyl diphosphate
Shewana3_3134016-1.627444peptidylprolyl isomerase, FKBP-type
Shewana3_3135119-1.819651lipoprotein signal peptidase
Shewana3_3136119-1.934032isoleucyl-tRNA synthetase
Shewana3_3137120-2.786262riboflavin kinase/FMN adenylyltransferase
Shewana3_3138023-3.760247integral membrane protein MviN
Shewana3_3139-119-2.829581hypothetical protein
Shewana3_3140-220-3.16248930S ribosomal protein S20
Shewana3_3141-124-3.362461ArsR family transcriptional regulator
Shewana3_3142024-3.015848peptidase M28
Shewana3_3143023-2.757367hypothetical protein
Shewana3_3144125-2.607908amino acid carrier protein
Shewana3_3145126-3.438150putative phosphoketolase
Shewana3_3146323-2.627650OmpA/MotB domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3117BINARYTOXINA310.008 Clostridial binary toxin A signature.
		>BINARYTOXINA#Clostridial binary toxin A signature.

Length = 454

Score = 30.8 bits (69), Expect = 0.008
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 2/48 (4%)

Query: 248 YFFVSPKRPELAAAILAGLENMISDGSFDEMFNRELKIDKLYRDAQFE 295
Y+F SP++ I +N IS F+E+ +E DKL++ F+
Sbjct: 133 YYFESPEKFAFNKEIRTENQNEISLEKFNEL--KETIQDKLFKQDGFK 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3120SALSPVBPROT300.030 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 29.7 bits (66), Expect = 0.030
Identities = 13/38 (34%), Positives = 16/38 (42%)

Query: 368 DVLTPHYNQVTTYVWERVVDFIKLHYCISDRTDSDFWL 405
DV P VT Y F +L Y + + DFWL
Sbjct: 123 DVSFPQSYTVTRYQPRTESSFYRLEYWVGNSNGDDFWL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3126BCTERIALGSPG361e-05 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 36.4 bits (84), Expect = 1e-05
Identities = 15/45 (33%), Positives = 26/45 (57%)

Query: 3 NKLFGFTLVELMVTIAVAAILLTIGVPSLISVYEGVRVNNNIAKI 47
+K GFTL+E+MV I + +L ++ VP+L+ E ++ I
Sbjct: 5 DKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDI 49


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3128BCTERIALGSPG615e-15 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 61.1 bits (148), Expect = 5e-15
Identities = 25/75 (33%), Positives = 46/75 (61%), Gaps = 2/75 (2%)

Query: 2 KKNRLQGFTLIEVMIAVVIVGILASIAYPSYIDYVVKSGRSEGVAAVMKVANLQEQYYLD 61
++ +GFTL+E+M+ +VI+G+LAS+ P+ + K+ + + V+ ++ + N + Y LD
Sbjct: 3 ATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLD 62

Query: 62 NRAYATDMTKLGLAA 76
N Y T T GL +
Sbjct: 63 NHHYPT--TNQGLES 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3132BCTERIALGSPG334e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.6 bits (74), Expect = 4e-04
Identities = 10/24 (41%), Positives = 18/24 (75%), Gaps = 2/24 (8%)

Query: 13 QRGFSLIEVLVALVIL--VIGLIG 34
QRGF+L+E++V +VI+ + L+
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVV 30


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3143PHPHTRNFRASE290.019 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 29.0 bits (65), Expect = 0.019
Identities = 10/36 (27%), Positives = 16/36 (44%), Gaps = 2/36 (5%)

Query: 24 RPDFLAYSQELIQVCQRLTPSDIATLMKVSDNIAGL 59
++E + + + LTPSD A L K + G
Sbjct: 147 TGSLATIAEETVIIAEDLTPSDTAQLNK--QFVKGF 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3146OMPADOMAIN1525e-45 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 152 bits (384), Expect = 5e-45
Identities = 89/380 (23%), Positives = 147/380 (38%), Gaps = 61/380 (16%)

Query: 7 MKNTLK--VVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGD----DNPYAWD 60
MK T V L +A +A ++ T WY GA LG + Y N + +N
Sbjct: 1 MKKTAIAIAVALAGFATVAQAAPKDNT-WYTGAKLGWSQYHDTGFINNNGPTHENQLGAG 59

Query: 61 IFAGYMFNDYFGAEIGYRDLGSADWTYAGIGNDADVKGATLGLVGVWPLGNRWSLSAEAG 120
F GY N Y G E+GY LG + + +G L +P+ + + G
Sbjct: 60 AFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLG 119

Query: 121 AMYYTLENNQRVGGVSSSYSENDFAPYFGAGVGYNFTDNLKLQAKYRRYENLDDNAGANA 180
M + + V G + +P F GV Y T + + +Y+ N+ G
Sbjct: 120 GMVWRADTKSNVYG---KNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNI----GDAH 172

Query: 181 IVPVNADSNYWGLELSYRFGTAAAAPVAA-AVVAATPVDSDNDGVYDDKDQCPATPATHK 239
+ D+ L +SYRFG AAPV A A A V + + + D
Sbjct: 173 TIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSD------------ 220

Query: 240 VDSVGCTIYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYM--NKNPEFTVEIAGH 297
+ F + A +K E +++L + + + +V + G+
Sbjct: 221 --------------------VLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGY 260

Query: 298 ASNVGKPEYNMVLSDKRADAVAKILVEKYGISQSRVTSNGYGITKPLVAGDS-------- 349
+G YN LS++RA +V L+ K GI ++++ G G + P V G++
Sbjct: 261 TDRIGSDAYNQGLSERRAQSVVDYLISK-GIPADKISARGMGESNP-VTGNTCDNVKQRA 318

Query: 350 --KEAHAANRRIEAIVTTTE 367
+ A +RR+E V +
Sbjct: 319 ALIDCLAPDRRVEIEVKGIK 338


43Shewana3_3237Shewana3_3242Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_32372152.047801MotA/TolQ/ExbB proton channel
Shewana3_32382182.002806biopolymer transport protein ExbD/TolR
Shewana3_32392172.112727periplasmic binding protein
Shewana3_32402181.944294transport system permease
Shewana3_32412180.952530hemin importer ATP-binding subunit
Shewana3_3242216-0.562103hypothetical protein
44Shewana3_3273Shewana3_3283Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3273-1203.403510hypothetical protein
Shewana3_32741223.801315cob(I)yrinic acid a,c-diamide
Shewana3_32751223.824710cobyric acid synthase
Shewana3_32761234.523784adenosylcobinamide kinase
Shewana3_3277-1214.442331cobalamin synthase
Shewana3_32780173.260475nicotinate-nucleotide--dimethylbenzimidazole
Shewana3_32790151.369975transport system permease
Shewana3_3280113-1.096009ABC transporter-like protein
Shewana3_3281214-0.883615phosphoglycerate mutase
Shewana3_3282213-0.998408B12-dependent methionine synthase
Shewana3_3283119-4.335386hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3280PF05272310.007 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.007
Identities = 10/58 (17%), Positives = 22/58 (37%), Gaps = 4/58 (6%)

Query: 14 ATSANMALKVSQLSWAIEGKTILSEISFALPQG----EMLGLIGPNGAGKSSLLRCLY 67
T + + + + ++ ++ + G + L G G GKS+L+ L
Sbjct: 560 KTPDDYKPRRLRYLQLVGKYILMGHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLV 617


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3282BCTERIALGSPD320.021 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 31.8 bits (72), Expect = 0.021
Identities = 14/71 (19%), Positives = 31/71 (43%), Gaps = 5/71 (7%)

Query: 354 SGLEPLTIDAQTLFVNVGERTN---VTGSAKFLKLIKEGKFEQALDVAREQVESGAQIID 410
+P+ + + + +TN VT + + ++ + LD+ R QV A I +
Sbjct: 298 QAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLE--RVIAQLDIRRPQVLVEAIIAE 355

Query: 411 INMDEGMLDGV 421
+ +G+ G+
Sbjct: 356 VQDADGLNLGI 366


45Shewana3_3378Shewana3_3387Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3378-2173.240328Na(+)-translocating NADH-quinone reductase
Shewana3_3379-2183.454946aldo/keto reductase
Shewana3_3380-2173.664624glyoxalase/bleomycin resistance
Shewana3_3381-2173.869013hypothetical protein
Shewana3_3382-2163.256070hypothetical protein
Shewana3_3383-2153.092286TonB-dependent receptor, plug
Shewana3_33840140.358951ATP-dependent RNA helicase DbpA
Shewana3_3385319-3.797368hypoxanthine phosphoribosyltransferase
Shewana3_3386327-6.513377hypothetical protein
Shewana3_3387423-6.049993hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3379HELNAPAPROT310.003 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 31.4 bits (71), Expect = 0.003
Identities = 26/123 (21%), Positives = 40/123 (32%), Gaps = 20/123 (16%)

Query: 112 AVDASLERLQIDTIDLY----QVHWPDRNTNFFG--ELFYDEQEIEQQTPILETLEALAE 165
V+ SL + LY + HW + +FF E F E ET++ +AE
Sbjct: 12 LVENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFE-----ELYDHAAETVDTIAE 66

Query: 166 VIRQGKVRYIGVSNETPWGLMK-YLQLAEKHGLPRIVTVQNPYNLLNRSFEVGMSEISHR 224
+ IG P +K Y + A + L ++ SE
Sbjct: 67 RLLA-----IGGQ---PVATVKEYTEHASITDGGNETSASEMVQALVNDYKQISSESKFV 118

Query: 225 EEL 227
L
Sbjct: 119 IGL 121


46Shewana3_3404Shewana3_3436Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3404-1173.460425TraR/DksA family transcriptional regulator
Shewana3_3405-1162.884164glutamyl-tRNA synthetase
Shewana3_34060132.527116poly(A) polymerase
Shewana3_34071122.0173112-amino-4-hydroxy-6-
Shewana3_34084211.1004003-methyl-2-oxobutanoate
Shewana3_34093190.525120pantoate--beta-alanine ligase
Shewana3_34104180.118308hypothetical protein
Shewana3_34114190.628988peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_34122210.099928curlin-associated protein
Shewana3_34131180.537578curlin-associated protein
Shewana3_3414-2131.476033LuxR family transcriptional regulator
Shewana3_3415-2132.014663hypothetical protein
Shewana3_3416-2132.126537D-3-phosphoglycerate dehydrogenase
Shewana3_3417-2152.529472glycine cleavage T protein (aminomethyl
Shewana3_3418-2152.433074response regulator receiver modulated metal
Shewana3_3419-1152.445670multi-sensor hybrid histidine kinase
Shewana3_34202193.176151amino acid carrier protein
Shewana3_34212202.818648ABC transporter-like protein
Shewana3_34222202.888984hypothetical protein
Shewana3_3423-1212.905539hypothetical protein
Shewana3_3424-1243.050293methylation site containing protein
Shewana3_3425-1212.840387methylation site containing protein
Shewana3_3426-1193.040679prepilin-type cleavage/methylation-like protein
Shewana3_3427-1182.929249prepilin-type cleavage/methylation-like protein
Shewana3_34280192.787789hypothetical protein
Shewana3_34291182.302720NapD family protein
Shewana3_34300162.254399nitrate reductase catalytic subunit
Shewana3_34311280.074809quinol dehydrogenase periplasmic component
Shewana3_3432123-0.769226quinol dehydrogenase membrane component
Shewana3_3433533-1.289722periplasmic nitrate reductase subunit NapB
Shewana3_3434532-1.464253hypothetical protein
Shewana3_3435430-1.457954LysR family transcriptional regulator
Shewana3_3436429-1.208730elongation factor G
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3405PF04605290.007 Virulence-associated protein D (VapD)
		>PF04605#Virulence-associated protein D (VapD)

Length = 125

Score = 29.5 bits (66), Expect = 0.007
Identities = 9/52 (17%), Positives = 17/52 (32%), Gaps = 2/52 (3%)

Query: 65 AADDILRTLEAYGFEWDDTVLYQSART--EAYQAKLDELLAEDNAYFCQCSR 114
I + + GFE Y S E ++ L + + +C +
Sbjct: 27 PYSLIKKFMLENGFEHRQYSGYTSKEPINERRVIRIVNKLTKKFTWLGECVK 78


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3409LPSBIOSNTHSS300.007 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 29.8 bits (67), Expect = 0.007
Identities = 7/26 (26%), Positives = 14/26 (53%)

Query: 34 HQGHITLVKEAAKKCDHVVVSIFVNP 59
GH+ +++ + D V V++ NP
Sbjct: 13 TFGHLDIIERGCRLFDQVYVAVLRNP 38


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3411SUBTILISIN2056e-62 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 205 bits (524), Expect = 6e-62
Identities = 104/298 (34%), Positives = 140/298 (46%), Gaps = 39/298 (13%)

Query: 128 WGMNNTGQNGGTVDADIDAPEAWEITTGSSDVVIGVIDTGVDYNHPDLQANMWVNGGEIP 187
N G + I AP W T G V + V+DTG D +HPDL+A + I
Sbjct: 16 EQQVNEIPRGVEM---IQAPAVWNQTRGR-GVKVAVLDTGCDADHPDLKARI------IG 65

Query: 188 GNGIDDDGNGVIDDVHGYSAVNNNGNPMDGNGHGTHVSGTIGAKGNNGVGVVGVNWDVKI 247
G DD G + D NGHGTHV+GTI A N GVVGV + +
Sbjct: 66 GRNFTDDDEGDPEI------------FKDYNGHGTHVAGTIAA-TENENGVVGVAPEADL 112

Query: 248 AACQFLDADGYGSTAGAIACLDYFTDLKVNHGVDIKATNNSWGGGSFSQALKDAIEAGGE 307
+ L+ G G I + Y + VDI + S GG L +A++
Sbjct: 113 LIIKVLNKQGSGQYDWIIQGIYYA----IEQKVDI--ISMSLGGPEDVPELHEAVKKAVA 166

Query: 308 AGILFVAAAGNDAVDND--ASPHYPSSYDSDVVLSIASTDRNDRMSDFSQWGLTSVDMGA 365
+ IL + AAGN+ +D YP Y+ V+S+ + + + S+FS VD+ A
Sbjct: 167 SQILVMCAAGNEGDGDDRTDELGYPGCYNE--VISVGAINFDRHASEFSNSN-NEVDLVA 223

Query: 366 PGTAILSTIPGGGYATYSGTSMATPHVTGAAALVWSLNP-----DLSPVEMKTLLMAS 418
PG ILST+PGG YAT+SGTSMATPHV GA AL+ L DL+ E+ L+
Sbjct: 224 PGEDILSTVPGGKYATFSGTSMATPHVAGALALIKQLANASFERDLTEPELYAQLIKR 281


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3418HTHFIS904e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.9 bits (223), Expect = 4e-22
Identities = 39/159 (24%), Positives = 65/159 (40%), Gaps = 6/159 (3%)

Query: 1 MDKATILVVDDTPENIDILVGILG-EDYKVKVAIDGPRALALVAKTLPDLILLDVMMPGM 59
M ATILV DD +L L Y V++ + +A DL++ DV+MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 NGYEVCKLLKQEPLTCHIPVIFVTALSEVADETQGFELGAVDYITKPVSAPVVKARVRTH 119
N +++ +K+ +PV+ ++A + + E GA DY+ KP + +
Sbjct: 61 NAFDLLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 120 LALYDQKRLLEQQVKERTQEL--EETRF-EIIRRLGRAA 155
LA ++ + + L EI R L R
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLM 157


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3419HTHFIS773e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.8 bits (189), Expect = 3e-16
Identities = 34/128 (26%), Positives = 54/128 (42%), Gaps = 5/128 (3%)

Query: 1284 ILVADDNATARDIMRTTLESMGFRVDTVRSGEEAVTRCIQQEYAVALIDWKMPNLDGIET 1343
ILVADD+A R ++ L G+ V + + + + D MP+ + +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 1344 AKQIKQQAKKAPRILMVSAHANQDFLSQIEELGLAGYISKPISASRLLDGIINSLGRAGV 1403
+IK+ P ++M SA + E G Y+ KP L +I +GRA
Sbjct: 66 LPRIKKARPDLPVLVM-SAQNTFMTAIKASEKGAYDYLPKPFD----LTELIGIIGRALA 120

Query: 1404 LPVRRQSE 1411
P RR S+
Sbjct: 121 EPKRRPSK 128



Score = 67.5 bits (165), Expect = 2e-13
Identities = 24/103 (23%), Positives = 42/103 (40%), Gaps = 2/103 (1%)

Query: 1425 RILLVEDNEMNLEVATEFLEQVGIILSIATNGQIALDKLEQQSFDLVLMDCQMPVMDGYQ 1484
IL+ +D+ V + L + G + I +N + DLV+ D MP + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 1485 ATQALRKRPELTELPVVAMTANAMAGDKEMCLRAGMNDHIAKP 1527
++K +LPV+ M+A G D++ KP
Sbjct: 65 LLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKP 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3424BCTERIALGSPG533e-12 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 53.4 bits (128), Expect = 3e-12
Identities = 19/64 (29%), Positives = 38/64 (59%)

Query: 5 RKGFTLIELMIAVAIIGILAAIAIPSFNEYLKQGRRFDAQQYLVSSAQALERHYSRNGLY 64
++GFTL+E+M+ + IIG+LA++ +P+ ++ + A +V+ AL+ + N Y
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHHY 66

Query: 65 PASQ 68
P +
Sbjct: 67 PTTN 70


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3425BCTERIALGSPG391e-06 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 39.1 bits (91), Expect = 1e-06
Identities = 15/31 (48%), Positives = 23/31 (74%)

Query: 2 AKRTKAGFTLVELLVAIAIIGILASIALPSY 32
A + GFTL+E++V I IIG+LAS+ +P+
Sbjct: 3 ATDKQRGFTLLEIMVVIVIIGVLASLVVPNL 33


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3427BCTERIALGSPG300.002 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 30.2 bits (68), Expect = 0.002
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 6/60 (10%)

Query: 3 LSAKKQQAGFSLSELMIAMV-LGLIIMLAVVNFF-----APLKATVEESKRLENAADALR 56
+ A +Q GF+L E+M+ +V +G++ L V N A + V + LENA D +
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYK 60


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3433PF06291260.025 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 26.2 bits (57), Expect = 0.025
Identities = 11/29 (37%), Positives = 15/29 (51%)

Query: 1 MKKILTLAAIVLAIGGCSGQQADSQTTPV 29
MKK+L AA+ + I GC+ Q P
Sbjct: 6 MKKMLFSAALAMLITGCAQQTFTVGNKPT 34


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3436TCRTETOQM5490.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 549 bits (1415), Expect = 0.0
Identities = 184/689 (26%), Positives = 300/689 (43%), Gaps = 70/689 (10%)

Query: 6 KYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGESTTDFMVQEAERGITIQSAAV 65
K NIG+ AHVDAGKTT TE +L +G I +LG V G + TD + E +RGITIQ+
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 66 SCFWKDHRFNVIDTPGHVDFTVEVYRSLKVLDGGIAVFCGSGGVEPQSETNWRYANESEV 125
S W++ + N+IDTPGH+DF EVYRSL VLDG I + GV+ Q+ + + +
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 126 ARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMVLPIGIEDEFTGVVDLLTRKAYVWDD 185
I F+NK+D+ G D V + K+ L+A ++
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ------------------------- 156

Query: 186 SGIPENFTVTDVPADMVDQVEEYREMLIESAVEQDDDLLEAYMEGEEPSIEDLKRCIRKG 245
V P V E + ++ +E +DDLLE YM G+ +L++
Sbjct: 157 -------KVELYPNMCVTNFTESEQ--WDTVIEGNDDLLEKYMSGKSLEALELEQEESIR 207

Query: 246 TRTMAFFPTFCGSAFKNKGMQLVLDAVVDYLPAPDEVDPQPLTDEEGNETGEYAIVSADE 305
+ FP + GSA N G+ +++ + + + T +E
Sbjct: 208 FHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSS--------THRGQSE----------- 248

Query: 306 SLKALAFKI-MDDRFGALTFVRIYAGRLKKGDTILNSATGKTERIGRMCEMYANDRIEIE 364
L FKI ++ L ++R+Y+G L D++ S K +I M + +I+
Sbjct: 249 -LCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKID 306

Query: 365 SAEAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVFPEPVISIAVAPKDKGGSEKMAIAI 424
A +G+I+ + + ++ L D K E + P P++ V P E + A+
Sbjct: 307 KAYSGEIVILQN-EFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDAL 365

Query: 425 GKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYRETIT 484
++ DP R D + E IL +G++ +++ +L+ Y VE+ + EP V Y E
Sbjct: 366 LEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYMERPL 425

Query: 485 QMVEDQYTHKKQSGGSGQFGKIEYIIRPGEPNSGFVFKSSVVGGNVPKEYWPAVEKGFAS 544
+ E YT + + + I + P SG ++SSV G + + + AV +G
Sbjct: 426 KKAE--YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRY 483

Query: 545 MMNTGTIAGFPVLDVEFELTDGAYHAVDSSAIAFEIAAKAAFRQSIAKAKPQLLEPIMKV 604
G + G+ V D + G Y++ S+ F + A Q + KA +LLEP +
Sbjct: 484 GCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSF 542

Query: 605 DVFSPEDNVGDVIGDLNRRRGMIKDQVAGVTGVRVKADVPLSEMFGYIGSLRTMTSGRGQ 664
+++P++ + D + I D V + ++P + Y L T+GR
Sbjct: 543 KIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSV 602

Query: 665 FSMEFSHYSPC----------PNSVSDKV 683
E Y PNS DKV
Sbjct: 603 CLTELKGYHVTTGEPVCQPRRPNSRIDKV 631


47Shewana3_3447Shewana3_3465Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3447-115-3.29611816S rRNA m(2)G 1207 methyltransferase
Shewana3_3448014-3.493474hypothetical protein
Shewana3_3449-112-0.800564hypothetical protein
Shewana3_34500182.281335hypothetical protein
Shewana3_3451-1193.021101hypothetical protein
Shewana3_3452-1193.273275hypothetical protein
Shewana3_3453-1152.595106peptidase M50
Shewana3_3454-1142.409839outer membrane efflux protein
Shewana3_3455-2142.288562ABC transporter-like protein
Shewana3_3456-2151.397963RND family efflux transporter MFP subunit
Shewana3_3457-2141.337600PHB depolymerase family esterase
Shewana3_3458-2131.168530TonB-dependent receptor
Shewana3_3459-2152.746645two component LuxR family transcriptional
Shewana3_3460-2133.173675multi-sensor hybrid histidine kinase
Shewana3_34610133.427434major facilitator superfamily transporter
Shewana3_3462-1123.852571alpha/beta hydrolase domain-containing protein
Shewana3_34630123.783683hypothetical protein
Shewana3_3464-1134.178022amidase
Shewana3_3465-1123.013661agmatine deiminase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3454RTXTOXIND371e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 37.1 bits (86), Expect = 1e-04
Identities = 24/186 (12%), Positives = 53/186 (28%), Gaps = 5/186 (2%)

Query: 74 QPELNQLISQVLSSNNDLTLATLTLQKARLQAGLARDDLYPQLSSNNTASVNKPLDGGSS 133
+P N ++ +++ + L +L A A D SS A + + S
Sbjct: 100 KPIENSIVKEIIVKEGESVRKGDVL--LKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 134 SRAFQANL-SVSYEVDLWGKVSANIDQAQWTALAS--LEDRESTAQSLVATTASLYWQIG 190
L + + + + + + + T+L ++ +
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 191 YLHERIELSNKSIEHSRQTLALTQRQYASGAVTELNVLESQRSLAGQEASHSQLLQQLVE 250
+ RI + L A+ + VLE + QL +
Sbjct: 218 TVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQ 277

Query: 251 AENALA 256
E+ +
Sbjct: 278 IESEIL 283


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3456RTXTOXIND642e-13 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 64.5 bits (157), Expect = 2e-13
Identities = 36/199 (18%), Positives = 67/199 (33%), Gaps = 26/199 (13%)

Query: 58 ANGMLQASKLVSVGAQVSGQIQSLPV------DLGQEVKKGDLIAQIDSLAQQNNLQNAL 111
N + ++ ++V A+++ V D + K IA+ L Q+N
Sbjct: 207 LNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQ-AIAKHAVLEQEN----KY 261

Query: 112 ASLKSINAQYRAKQAQIRQAKLEYTRQQEMLADKASSRADFETAEATLTVYQAELEQLQA 171
+ Y+++ QI L + +++ F+ +L Q
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLV------TQLFKNEILD------KLRQTTD 309

Query: 172 QKQQAEINVDSARIDLGYTKITAPMDGTVVYSAV-EVGQTVNANQTTPTIVEMAQLDTMT 230
+ + + I AP+ V V G V +T IV + DT+
Sbjct: 310 NIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIV--PEDDTLE 367

Query: 231 VKAQISEADIVNVHPGQAV 249
V A + DI ++ GQ
Sbjct: 368 VTALVQNKDIGFINVGQNA 386



Score = 48.3 bits (115), Expect = 3e-08
Identities = 31/182 (17%), Positives = 70/182 (38%), Gaps = 15/182 (8%)

Query: 7 MKKSSKRKLILILSGLVLLGGGAYFLLHKPEAAPSYVTEPVKRGDIENSVLANGMLQAS- 65
++ R+ L+ ++G + S + + +E ANG L S
Sbjct: 49 IETPVSRRPRLV--AYFIMGFLVIAFIL------SVLGQ------VEIVATANGKLTHSG 94

Query: 66 KLVSVGAQVSGQIQSLPVDLGQEVKKGDLIAQIDSLAQQNNLQNALASLKSINAQYRAKQ 125
+ + + ++ + V G+ V+KGD++ ++ +L + + +SL + Q
Sbjct: 95 RSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQ 154

Query: 126 AQIRQAKLEYTRQQEMLADKASSRADFETAEATLTVYQAELEQLQAQKQQAEINVDSARI 185
R +L + ++ + E ++ + + Q QK Q E+N+D R
Sbjct: 155 ILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRA 214

Query: 186 DL 187
+
Sbjct: 215 ER 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3459HTHFIS642e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.5 bits (157), Expect = 2e-14
Identities = 40/167 (23%), Positives = 68/167 (40%), Gaps = 8/167 (4%)

Query: 1 MSQIKVAIADDHPLFRTALTQAVLKNVNTAEVLEAENFQELITLVENNPDIELIFLDLHM 60
M+ + +ADD RT L QA L +V N L + +L+ D+ M
Sbjct: 1 MTGATILVADDDAAIRTVLNQA-LSRAG-YDVRITSNAATLWRWIAAGD-GDLVVTDVVM 57

Query: 61 PGNEGFTGLTLLQNHFPDIAVIMVSSDDQPEIIRKAINFGASAFIPKSASLTQISTAIAT 120
P F L ++ PD+ V+++S+ + KA GA ++PK LT++ I
Sbjct: 58 PDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117

Query: 121 VLEGEVWLPEHTDINVDQQ-----TAAEHQRLAKQLAQLTPQQYTVL 162
L P + + +A Q + + LA+L T++
Sbjct: 118 ALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLM 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3460HTHFIS399e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 39.4 bits (92), Expect = 9e-05
Identities = 14/71 (19%), Positives = 30/71 (42%), Gaps = 2/71 (2%)

Query: 1055 ISVLVIDNDELMLKAISSLLLGWGCHVLTARDKASAEQQLTQQVLPKLIIADYHLDDDQN 1114
++LV D+D + ++ L G V + A+ + + L++ D + D+ N
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWI-AAGDGDLVVTDVVMPDE-N 61

Query: 1115 GVDLVQSLLTH 1125
DL+ +
Sbjct: 62 AFDLLPRIKKA 72


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3461TCRTETB290.024 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.5 bits (66), Expect = 0.024
Identities = 27/107 (25%), Positives = 40/107 (37%), Gaps = 4/107 (3%)

Query: 252 GIVGTIAGILYSRKQPLRLPIIRLSGLLIFLTVLGLSFGTAPWLQTLCAI-VLGFCIFLP 310
I G I GIL R+ PL + I ++ L + T W T+ + VLG F
Sbjct: 307 IIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTK 366

Query: 311 VTALVSIPHELPKMTSQKITVIFSLFWSISYLISTLVLWLFGKLVDI 357
+ L Q+ SL S+L + + G L+ I
Sbjct: 367 TVISTIVSSSL---KQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSI 410


48Shewana3_3492Shewana3_3502Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3492226-4.104459hypothetical protein
Shewana3_3493329-5.481931hypothetical protein
Shewana3_3494225-3.244385hypothetical protein
Shewana3_3495327-3.022429hypothetical protein
Shewana3_3496228-2.481932cell division protein FtsK
Shewana3_3497123-0.253965hypothetical protein
Shewana3_34980201.045237hypothetical protein
Shewana3_34990253.151152glycine dehydrogenase
Shewana3_3500-2153.287111glycine cleavage system protein H
Shewana3_3501-2143.525036glycine cleavage system aminomethyltransferase
Shewana3_35020183.7669702-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3495TOXICSSTOXIN260.025 Staphylococcal toxic shock syndrome toxin signature.
		>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature.

Length = 234

Score = 25.8 bits (56), Expect = 0.025
Identities = 14/59 (23%), Positives = 27/59 (45%), Gaps = 5/59 (8%)

Query: 8 PALKQVQQMLKANQASINGELEELNRQWYALRDNYEGEGAENTEGMVMDLGSWLEEYTN 66
P Q++K +AS N +++L + + D + N+E + LGS + T+
Sbjct: 26 PVPLSSNQIIKTAKASTNDNIKDLLDWYSSGSDTF-----TNSEVLDNSLGSMRIKNTD 79


49Shewana3_3551Shewana3_3566Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3551-2203.351617hypothetical protein
Shewana3_3552-1213.6206554-carboxymuconolactone decarboxylase
Shewana3_3553-1213.454118putative nonspecific acid phosphatase
Shewana3_35540224.062525hypothetical protein
Shewana3_3555-1203.299756von Willebrand factor type A domain-containing
Shewana3_35560171.663473von Willebrand factor type A domain-containing
Shewana3_3557-1160.023460hypothetical protein
Shewana3_3558-113-0.929757hypothetical protein
Shewana3_3559-114-0.116966ubiquinol--cytochrome-c reductase
Shewana3_3560118-4.753691radical SAM domain-containing protein
Shewana3_3562122-4.615050transcriptional regulator
Shewana3_3563125-4.277825hypothetical protein
Shewana3_3564021-3.027552hypothetical protein
Shewana3_3565-118-2.295699IS4 family transposase
Shewana3_3566019-3.252780hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3559HTHFIS330.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 32.9 bits (75), Expect = 0.002
Identities = 37/145 (25%), Positives = 62/145 (42%), Gaps = 17/145 (11%)

Query: 30 RTVIRSLILGLLCSGHVLLEGLPGTAKTRSVKAL------ANALAISFGRIQFTPDLLPS 83
+ + R L + +++ G GT K +AL N ++ DL+ S
Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206

Query: 84 DVTGTE--VLHEAEGKSTLRFQP---GPVFNQIVLADEINRAPAKVQAALLEAMAEGTIT 138
++ G E A+ +ST RF+ G +F DEI P Q LL + +G T
Sbjct: 207 ELFGHEKGAFTGAQTRSTGRFEQAEGGTLF-----LDEIGDMPMDAQTRLLRVLQQGEYT 261

Query: 139 -VAGQTHVLPELFMVLATQNPIEQE 162
V G+T + ++ +V AT ++Q
Sbjct: 262 TVGGRTPIRSDVRIVAATNKDLKQS 286


50Shewana3_3775Shewana3_3800Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3775-116-3.169073GTP cyclohydrolase I
Shewana3_3776021-3.406336orotate phosphoribosyltransferase
Shewana3_3777223-4.845912ribonuclease PH
Shewana3_3778228-6.347705hypothetical protein
Shewana3_3779432-8.184027phage integrase family protein
Shewana3_3780536-9.142059hypothetical protein
Shewana3_3781636-8.868525hypothetical protein
Shewana3_3782537-9.400077hypothetical protein
Shewana3_3783536-8.929681hypothetical protein
Shewana3_3784432-8.222685hypothetical protein
Shewana3_3785221-6.139729hypothetical protein
Shewana3_3786017-5.051437chemotaxis protein MotB-like protein
Shewana3_3787015-3.813824hypothetical protein
Shewana3_3788-117-3.172183SNF2-like protein
Shewana3_3789-218-0.028388hypothetical protein
Shewana3_3790-116-0.826792HsdR family type I site-specific
Shewana3_3791-116-1.951968hypothetical protein
Shewana3_3792-115-1.832978hypothetical protein
Shewana3_3793-115-2.639572restriction modification system DNA specificity
Shewana3_3794-112-1.915171type I restriction-modification system, M
Shewana3_3795021-3.677423HNH endonuclease
Shewana3_3796022-3.652444hypothetical protein
Shewana3_3797-124-3.740795nucleotidase
Shewana3_3798-128-4.682042hypothetical protein
Shewana3_3799-126-4.267457hypothetical protein
Shewana3_3800022-4.060775LysR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3785CHANLCOLICIN310.019 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 30.8 bits (69), Expect = 0.019
Identities = 58/339 (17%), Positives = 130/339 (38%), Gaps = 26/339 (7%)

Query: 340 NQSVEAMQLTMSNFVEQLQKSQAESGDREKALIADISHQVSKLSSQSEDIHQKLTSYVEN 399
++S A+ T QL+K+QAE R KA +K + + + Q+L V
Sbjct: 45 SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAE----AQAKAKANRDALTQRLKDIVNE 100

Query: 400 QIGKISSQMQIREEASAKRDSELVNVIGQQVNELVNNSRRQGELLTSFVETQLNNLTKSF 459
+ +S+ SA + N Q +E + + + E E ++
Sbjct: 101 ALRHNASR-----TPSATELAHANNAAMQAEDERLRLA-KAEEKARKEAEAAEKAFQEAE 154

Query: 460 DERDKRSTELETTRNN----KIEKQTEAIVKISNELISTVEKSVSEQLAAVKHLVSQGET 515
R + E T + E++ A + + + +K +S + V + + +T
Sbjct: 155 QRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKT 214

Query: 516 LQNSVNASVEAAAQATQAMKESSIELRVSADHMRVLSSHVNDAGNKLSGAIKSAVDSTAD 575
L + +++S+ A + + EL ++ + L V + + +++
Sbjct: 215 LNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFE-- 272

Query: 576 LANQNQISAQRI-ENARESLMKDVSRFSELSDQIKALITSASSTFTELKSTQRDFIGNLK 634
A + ++ A +I E ++ + +R ++I A IT +++ + + I +
Sbjct: 273 -ATRRRVGAGKIREEKQKQVTASETRI----NRINADITQIQKAISQVSNNRNAGIARVH 327

Query: 635 EEVESLSRKMTDMLEEYSQQANGQTAEHLKIWSQSVTDY 673
E E+L + ++L + A T Q++T+
Sbjct: 328 EAEENLKKAQNNLLNSQIKDAVDATVSFY----QTLTEK 362


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3788PF07472310.027 Fucose-binding lectin II
		>PF07472#Fucose-binding lectin II

Length = 245

Score = 30.8 bits (69), Expect = 0.027
Identities = 19/81 (23%), Positives = 44/81 (54%), Gaps = 10/81 (12%)

Query: 683 QSFRHTYISPILQAAGDEIESVRASLGRKLREKVGALMLRRVKEDNLERLPKKNMFVGLE 742
Q F H + +LQ AG++ +V+A+ + + +++ M ++ +E+LP+ ++FV +
Sbjct: 3 QPFTHDDLYALLQLAGNDATAVQANGDQAVLDRMRQFMTTQL----VEKLPQYDVFVDIA 58

Query: 743 PTEWQYEPKLHSIMDSYQQKV 763
+ ++ + S+Q KV
Sbjct: 59 TIPYSFD------VGSWQNKV 73


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3800PHPHTRNFRASE280.041 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 28.2 bits (63), Expect = 0.041
Identities = 22/96 (22%), Positives = 43/96 (44%), Gaps = 22/96 (22%)

Query: 70 RPAVQLTQEGLDAISELQQTPKGNLRISVPMVFGRLYIAPLIAEFLKRYPDIQLQMQMDD 129
+ + TQ L A+ L+ + GNL++ PM+ + E Q + M +
Sbjct: 367 KQDIFRTQ--LRAL--LRASTYGNLKVMFPMI-------ATLEEL------RQAKAIMQE 409

Query: 130 KTTDLIAGGFDLA--IRIG---ELPDSSLIARKIAP 160
+ L++ G D++ I +G E+P +++ A A
Sbjct: 410 EKDKLLSEGVDVSDSIEVGIMVEIPSTAVAANLFAK 445


51Shewana3_3843Shewana3_3862Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3843220-2.164353hypothetical protein
Shewana3_3844335-7.018807DsrE family protein
Shewana3_3845338-8.072214hypothetical protein
Shewana3_3846442-9.763248hypothetical protein
Shewana3_3847347-10.861050hypothetical protein
Shewana3_3848347-11.364869RND family efflux transporter MFP subunit
Shewana3_3849344-10.501096acriflavin resistance protein
Shewana3_3850339-9.602265TetR family transcriptional regulator
Shewana3_3851233-8.094191diguanylate cyclase
Shewana3_3852430-6.993745CRISPR-associated Csy4 family protein
Shewana3_3853430-6.793341CRISPR-associated Csy3 family protein
Shewana3_3854430-6.656816hypothetical protein
Shewana3_3855529-6.845054hypothetical protein
Shewana3_3856628-7.279292hypothetical protein
Shewana3_3857629-8.985515hypothetical protein
Shewana3_3858434-9.751408hypothetical protein
Shewana3_3859330-8.597967XRE family transcriptional regulator
Shewana3_3860123-8.035883hypothetical protein
Shewana3_3861018-6.024904hypothetical protein
Shewana3_3862-116-4.785480integrase catalytic subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3846TCRTETB290.029 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 28.7 bits (64), Expect = 0.029
Identities = 17/125 (13%), Positives = 46/125 (36%), Gaps = 12/125 (9%)

Query: 107 YWPALITLFLAMLDMVVRLNLHKNALWFQAEFFKHRQDIDLSHWSLA----LPLLLFIQT 162
+W L+ + + + V L + + + + D+ L + +LF +
Sbjct: 167 HWSYLLLIPMITIITVPFL------MKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTS 220

Query: 163 ATY-FFALWRLFQRHKLRQQQRNEAPQQTLKLIRFR-WLFGLTLAMLFNWLLRVFVVVLP 220
+ F + L ++ ++ P L + ++ G+ + + FV ++P
Sbjct: 221 YSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVP 280

Query: 221 FYLGD 225
+ + D
Sbjct: 281 YMMKD 285


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3848RTXTOXIND476e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.1 bits (112), Expect = 6e-08
Identities = 19/117 (16%), Positives = 47/117 (40%), Gaps = 15/117 (12%)

Query: 59 VSSVKEAVLAFEVPGKVIKLHVKEGDFVKQDTVLAEIDDRDYQAQLDSAKSDLIVAKSDF 118
S + + E V ++ VKEG+ V++ VL ++ +A +S L+ A+ +
Sbjct: 92 HSGRSKEIKPIE-NSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ 150

Query: 119 QRYE---KALKADAVTPQAF-----------QQAKRNLEVAQAAFNQADKALTETKL 161
RY+ ++++ + + ++ R + + F+ + +L
Sbjct: 151 TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKEL 207



Score = 42.5 bits (100), Expect = 2e-06
Identities = 37/190 (19%), Positives = 74/190 (38%), Gaps = 29/190 (15%)

Query: 100 YQAQLDSAKSDLIVAKSDFQRYEKALKADAVTPQAFQQAKRNLEVAQAAFNQADKALTET 159
Y++QL+ +S+++ AK ++Q + K + + Q N+ + + ++ +
Sbjct: 271 YKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLR--QTTDNIGLLTLELAKNEERQQAS 328

Query: 160 KLIAPFAGRVVTREI-DLFATVHAKQPIMQL-HSESAYEMVVSVPESDWAQGERVNSAAD 217
+ AP + +V ++ V + +M + + E+ V D V A
Sbjct: 329 VIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGF-INVGQNAI 387

Query: 218 IKLDNQLFVTLTAFPDKRF---EGKITEFSGQADAAT--RT---YKVKVAFSVP------ 263
IK++ AFP R+ GK+ + DA R + V ++
Sbjct: 388 IKVE--------AFPYTRYGYLVGKVKNIN--LDAIEDQRLGLVFNVIISIEENCLSTGN 437

Query: 264 DKTPISSGMT 273
P+SSGM
Sbjct: 438 KNIPLSSGMA 447


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3849ACRIFLAVINRP459e-147 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 459 bits (1182), Expect = e-147
Identities = 220/1050 (20%), Positives = 445/1050 (42%), Gaps = 67/1050 (6%)

Query: 3 IASVAINNRVVTLVLTVVMLIAGLYIFNGMSRLEDPEFTIKDALIITPYNGASALEVEQE 62
+A+ I + VL +++++AG + + P + Y GA A V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VTELLEKTVQQLGELDKVTSKSER-GLSTITVTIKEQYNKETLPQVWNKLRQKIDDVKYY 121
VT+++E+ + + L ++S S+ G TIT+T + + + +++ K+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPD---IAQVQVQNKLQLATPL 117

Query: 122 LPPGA-GPSLVIDDYGDVYSIFMVV----SGDGYSFKELKTYVND-LQQQLLLVNGVGKI 175
LP + ++ S MV G + ++ YV ++ L +NGVG +
Sbjct: 118 LPQEVQQQGISVEKSSS--SYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDV 175

Query: 176 TTFGEKSEAIYIEFNRSRMAQLGISPEIVAAQLNGKGLVVDAGRAHVGSS------SIAV 229
FG + A+ I + + + ++P V QL + + AG+ + + ++
Sbjct: 176 QLFGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASI 234

Query: 230 STTGGFTKVSDFEKLLI--THDTKQFYLSDIAKVSRGYVSPSTQLINFDGKAGIGLGIST 287
F +F K+ + D L D+A+V G + +GK GLGI
Sbjct: 235 IAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELG-GENYNVIARINGKPAAGLGIKL 293

Query: 288 VSGGNTVDMGEAVLAKLSELESQRPAGIEFGYVSLQSEGVKEAISGFTSSLAEAVIIVIV 347
+G N +D +A+ AKL+EL+ P G++ Y + V+ +I +L EA+++V +
Sbjct: 294 ATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFL 353

Query: 348 VLLFFMG-LRSGLLIGFVLILTIAGSFIFLAPMGVALERISLGALIIALGMLVDNAIVVV 406
V+ F+ +R+ L+ + + + G+F LA G ++ +++ +++A+G+LVD+AIVVV
Sbjct: 354 VMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVV 413

Query: 407 DGILIRMQK-GESAESAAPRVVNQSAWPLLGATLIAILAFAAIGTSNDATGEYCRSLFQV 465
+ + M + + A + ++Q L+G ++ F + +TG R
Sbjct: 414 ENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSIT 473

Query: 466 VMVSLLLSWVTAVTITPLLCVMYLKAPKSTDKTSPYQGTFYT-----------KYRGLLA 514
++ ++ LS + A+ +TP LC LK + + G F+ Y +
Sbjct: 474 IVSAMALSVLVALILTPALCATLLKPVSAEHHENK--GGFFGWFNTTFDHSVNHYTNSVG 531

Query: 515 SSIRHRYLSSASIIGIFALSLWGFSFVQQNFFPSSTRAQFMVDFWLPQGTHIEETQKHAE 574
+ I A + F + +F P + F+ LP G E TQK +
Sbjct: 532 KILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLD 591

Query: 575 SVENYLGNL--ANVEHVTTTIGEGALRFLLTYQPQQSNSSYAQF-LVDVDDYTVIKTLIP 631
V +Y ANVE V T G ++ Q N+ A L ++ +
Sbjct: 592 QVTDYYLKNEKANVESVFTVNG-------FSFSGQAQNAGMAFVSLKPWEERNGDENSAE 644

Query: 632 KIEVELLQKY---PDALVYASP----FELGTGTAGKIQ-ARISGPDTDVLRETSDKVLEV 683
+ + D V ELGT T + +G D L + +++L +
Sbjct: 645 AVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGM 704

Query: 684 FSKE-SNTKGVRTDWRNKQLYIEAVLAEEQANINGINRGMVAEAIKESFEGVTTGVYREN 742
++ ++ VR + + + +E+A G++ + + I + G + +
Sbjct: 705 AAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDR 764

Query: 743 DLLLPIIIRANENERSDITNIENVQIWSPNAQKMIPLRQVVQSFETKFEDGLILRRNRER 802
+ + ++A+ R +++ + + S N + M+P + + + R N
Sbjct: 765 GRVKKLYVQADAKFRMLPEDVDKLYVRSANGE-MVPFSAFT-TSHWVYGSPRLERYNGLP 822

Query: 803 TITIFADPVTG-TASELLATLKPQVEAIKIPPGYTLEWGGEYEDSSKAEKGLASSIPIFI 861
++ I + G ++ + +A ++ K+P G +W G + + + I
Sbjct: 823 SMEIQGEAAPGTSSGDAMALMENLAS--KLPAGIGYDWTGMSYQERLSGNQAPALVAISF 880

Query: 862 LSMILITIVIFNSLKQTLVIWLCVPLALIGVTAGMLATNQPFGFMALLGFLSLIGMLIKN 921
+ + L ++ S + + L VPL ++GV NQ ++G L+ IG+ KN
Sbjct: 881 VVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKN 940

Query: 922 AIVLVDEIN-LEQSQGKSLINSILDSGVSRLRPVAMAALTTALGMIPLIFD-----AFFV 975
AI++V+ L + +GK ++ + L + RLRP+ M +L LG++PL
Sbjct: 941 AILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQN 1000

Query: 976 SMAVTIISGLMFATALTMIVLPIVYALIFK 1005
++ + ++ G++ AT L + +P+ + +I +
Sbjct: 1001 AVGIGVMGGMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3850HTHTETR654e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 64.6 bits (157), Expect = 4e-15
Identities = 30/184 (16%), Positives = 72/184 (39%), Gaps = 9/184 (4%)

Query: 19 DEKRNELLCTAINLLVTDGYAGLSMRNLASKAQVTTGAITYYFSNKSELMKGVSDELFNR 78
E R +L A+ L G + S+ +A A VT GAI ++F +KS+L + + +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 79 FSLLLA-------KNTEIDIKQAVEEWINWTYSDGGE-IWKAFLQVQFYAAEDKTFIDYA 130
L + +++ + + T ++ + + + + + A
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 131 QKRYDI-FISQLKHFIESGQKSKQIRNDIPAEILAEQLSAMGDGMMIMQSIYPERLSIEK 189
Q+ + +++ ++ ++K + D+ A + G+M P+ ++K
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKK 189

Query: 190 IAQF 193
A+
Sbjct: 190 EARD 193


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3852BINARYTOXINB280.021 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 28.5 bits (63), Expect = 0.021
Identities = 22/86 (25%), Positives = 35/86 (40%), Gaps = 7/86 (8%)

Query: 118 RLKRLEKRALVRGETFNPIKNIQPREFYTFHRIAISSGSNKQDYLLHIQKMVAK----EQ 173
+ LEK +R +T NI F R+ + +GSN + L IQ+ A+ +
Sbjct: 467 QFLELEKTKQLRLDTDQVYGNIATYNFEN-GRVRVDTGSNWSEVLPQIQETTARIIFNGK 525

Query: 174 TEPLFSSYGVASNLQ--LNGTVPELS 197
L A N L T P+++
Sbjct: 526 DLNLVERRIAAVNPSDPLETTKPDMT 551


52Shewana3_3911Shewana3_3917Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_39112241.462992hypothetical protein
Shewana3_39122241.226269hypothetical protein
Shewana3_39133251.0236644Fe-4S ferredoxin
Shewana3_39143290.906278cytoplasmic chaperone TorD family protein
Shewana3_39154351.088771hypothetical protein
Shewana3_39164361.116590formate dehydrogenase subunit alpha
Shewana3_39174321.1449364Fe-4S ferredoxin
53Shewana3_3962Shewana3_3976Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3962214-0.132873formate-dependent nitrite reductase
Shewana3_39631160.618342hypothetical protein
Shewana3_39640140.953810LysR family transcriptional regulator
Shewana3_39651131.8206802-succinyl-5-enolpyruvyl-6-hydroxy-3-
Shewana3_39660131.110729alpha/beta hydrolase fold domain-containing
Shewana3_39672121.505013O-succinylbenzoate synthase
Shewana3_39682121.449366o-succinylbenzoate--CoA ligase
Shewana3_39693130.537812RNA polymerase factor sigma-32
Shewana3_39703100.850486cell division protein FtsX
Shewana3_39713100.754617cell division ATP-binding protein FtsE
Shewana3_39725130.412089signal recognition particle-docking protein
Shewana3_3973215-1.103519putative methyltransferase
Shewana3_3974216-2.352031hypothetical protein
Shewana3_3975317-1.546833transcriptional regulator
Shewana3_3976420-2.091853isochorismatase hydrolase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3972IGASERPTASE655e-13 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 65.1 bits (158), Expect = 5e-13
Identities = 31/162 (19%), Positives = 59/162 (36%), Gaps = 10/162 (6%)

Query: 99 QAAEAARLAAEQAEAQRIAEEQAARLAEQQAVEAARLAAEQAQAEQLAAEKAEAERIAAE 158
QA+ + +A A ++ + AE ++ E E
Sbjct: 993 DTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVE 1052

Query: 159 QAAKAQAEAQRVAEEQAVRLAEQQAVEAARLAAEQAQAEQLAAEKAEAERIAAEQAAAAQ 218
+ + E E A + V+A E AQ+ E E + ++ A +
Sbjct: 1053 KNEQDATETTAQNREVA--KEAKSNVKANTQTNEVAQSGS---ETKETQTTETKETATVE 1107

Query: 219 AEAEAQAEAERIA-----AAQIQAEQPLEQQPEPQAKPAKES 255
E +A+ E E+ +Q+ +Q + +PQA+PA+E+
Sbjct: 1108 KEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREN 1149



Score = 61.2 bits (148), Expect = 8e-12
Identities = 32/187 (17%), Positives = 61/187 (32%), Gaps = 13/187 (6%)

Query: 74 AEQAAKAQAEAQRVAEEQAASLAEQQAAEAARLAAEQAEAQRIAEEQAARLAEQQAVEAA 133
QA+ V +A A +E E + + E + VE
Sbjct: 997 ITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQ--ESKTVEKN 1054

Query: 134 RLAAEQAQAEQ-LAAEKAEAERIAAEQAAKAQAEAQRVAEEQAVRLAEQQAVEAARLAAE 192
A + A+ A++A++ A Q + E Q E VE +
Sbjct: 1055 EQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVE------K 1108

Query: 193 QAQAEQLAAEKAEAERIAAE----QAAAAQAEAEAQAEAERIAAAQIQAEQPLEQQPEPQ 248
+ +A+ + E ++ ++ Q + + +A+ E I+ Q
Sbjct: 1109 EEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADT 1168

Query: 249 AKPAKES 255
+PAKE+
Sbjct: 1169 EQPAKET 1175



Score = 60.8 bits (147), Expect = 1e-11
Identities = 37/232 (15%), Positives = 75/232 (32%), Gaps = 33/232 (14%)

Query: 17 DEVVEQTPVSTPSQTEQAEALAKQQAEEARLAAEKAAAEQALADKLAAEKAEAERIAAEQ 76
++ V+ T ++TP+ + A + + E + A +E AE
Sbjct: 989 NQTVDTTNITTPNNIQ-----ADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAEN 1043

Query: 77 AAKAQAEAQRVAEEQAASLAEQQAAEAARLAAEQAEAQRIAEEQAARLAEQQAVEAARLA 136
+ + E + EQ A E E A+ + V+A
Sbjct: 1044 SKQ---------ESKTVEKNEQDATETTAQNREVAKE------------AKSNVKANTQT 1082

Query: 137 AEQAQAEQLAAEKAEAERIAAEQAAKAQAEAQRVAEEQAVRLAEQQAVEAARLAAEQAQA 196
E AQ+ E E + ++ A E + A+ + + E V ++++ +Q Q+
Sbjct: 1083 NEVAQSGS---ETKETQTTETKE--TATVEKEEKAKVETEKTQEVPKV-TSQVSPKQEQS 1136

Query: 197 EQ-LAAEKAEAERIAAEQAAAAQAEAEAQAEAERIAAAQIQAEQPLEQQPEP 247
E + E Q++ A+ E+ A + +
Sbjct: 1137 ETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTT 1188


54Shewana3_4000Shewana3_4009Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_4000-120-4.296906polysaccharide deacetylase
Shewana3_4001-125-5.482196MATE efflux family protein
Shewana3_4003032-7.445054putative DNA uptake protein
Shewana3_4004-131-6.565657flavocytochrome c
Shewana3_4005-128-5.850158hypothetical protein
Shewana3_4006025-3.065113histidine kinase A domain-containing protein
Shewana3_4007-3130.644112two component transcriptional regulator
Shewana3_4008-3122.063359LuxR family transcriptional regulator
Shewana3_4009-2123.061566competence protein ComF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4007HTHFIS765e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.6 bits (186), Expect = 5e-18
Identities = 30/124 (24%), Positives = 61/124 (49%), Gaps = 1/124 (0%)

Query: 3 VLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQADMLISQQQFDVVILDLNLPDGDGIKA 62
+L+ +D+ + + Q L +G + N A I+ D+V+ D+ +PD +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 63 CERWKAQCITSPIIMLTARSSLRERLDGFAVGADDYLVKPFAMEELVARL-KVVAQRRPA 121
R K P+++++A+++ + GA DYL KPF + EL+ + + +A+ +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 122 PQRL 125
P +L
Sbjct: 126 PSKL 129


55Shewana3_4090Shewana3_4096Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_4090022-6.997336hypothetical protein
Shewana3_4091132-11.238042phosphopantetheine adenylyltransferase
Shewana3_4092439-13.756936hypothetical protein
Shewana3_4093231-11.471665group 1 glycosyl transferase
Shewana3_4094332-11.395639capsule biosynthesis phosphatase
Shewana3_4095126-8.634409hypothetical protein
Shewana3_4096122-4.601282hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4091LPSBIOSNTHSS2262e-79 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 226 bits (578), Expect = 2e-79
Identities = 80/155 (51%), Positives = 113/155 (72%)

Query: 5 AIYPGTFDPITNGHADLIERAAKLFKHVIIGIAANPSKQPRFTLEERVELVNRVTAHLDN 64
AIYPG+FDPIT GH D+IER +LF V + + NP+KQP F+++ER+E + + AHL N
Sbjct: 3 AIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLPN 62

Query: 65 VEVVGFSGLLVDFAKEQRASVLVRGLRAVSDFEYEFQLANMNRRLSPDLESVFLTPAEEN 124
+V F GL V++A++++A ++RGLR +SDFE E Q+AN N+ L+ DLE+VFLT + E
Sbjct: 63 AQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTEY 122

Query: 125 SFISSTLVKEVALHGGDVSQFVHPEVASALTAKLN 159
SF+SS+LVKEVA GG+V FV VA+AL + +
Sbjct: 123 SFLSSSLVKEVARFGGNVEHFVPSHVAAALYDQFH 157


56Shewana3_0150Shewana3_0157N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_01500150.829748general secretion pathway protein C
Shewana3_01510170.999815general secretion pathway protein D
Shewana3_0152-1191.769738type II secretion system protein E (GspE)
Shewana3_0153-1180.705129general secretion pathway protein F
Shewana3_0154-1160.334262general secretion pathway protein G
Shewana3_0155-2150.979728general secretion pathway protein H
Shewana3_0156-1130.767744general secretion pathway protein I
Shewana3_0157-1131.042695general secretion pathway protein J
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0150BCTERIALGSPC1825e-58 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 182 bits (462), Expect = 5e-58
Identities = 72/295 (24%), Positives = 138/295 (46%), Gaps = 33/295 (11%)

Query: 8 IAKAAGIPHKPLSQVVFWFGFILSLLLAAQITWKLVPSTSSPTAWSPTAVTTTGKGAGQV 67
I+K + + +++F+ +L A I W++ ++P + +V T A Q
Sbjct: 3 ISKLPPLSPSVIRRILFYLLMLLFCQQLAMIFWRIGLPDNAPVS----SVQITPAQARQQ 58

Query: 68 DLAGLQQLALFGRADAKSDKPKVEVVETVTDAPKTSLSIQLTGVVASTADQKGLAIIESS 127
+ L LFG + K+ ++ +++ P ++L++ LTGV+A D + +AII
Sbjct: 59 PV-TLNDFTLFGVSPEKNKAGALDA-SQMSNLPPSTLNLSLTGVMAGDDDSRSIAIISKD 116

Query: 128 GSQETYSLGDKIKGTSASLKEVYADRIIITNAGRYETLMLDGLVYTSQSPANQQLQKAKS 187
Q + + +++ G +A + + DR+++ GRYE L L +
Sbjct: 117 NEQFSRGVNEEVPGYNAKIVSIRPDRVVLQYQGRYEVLGLYSQEDSGSDGVPG------- 169

Query: 188 EKAEVVSRVDQRKNTEISQELAESRSELLADPSKITDYIAISPVRQGESVVGYRLNPGKD 247
A+V ++ QR + ++DY++ SP+ + GYRLNPG
Sbjct: 170 --AQVNEQLQQR------------------ASTTMSDYVSFSPIMNDNKLQGYRLNPGPK 209

Query: 248 VNLFKQAGFKPNDLAKSINGYDLTVMSQALEMMSQLPELTEVSIMVEREGQLVEI 302
+ F + G + ND+A ++NG DL QA + M ++ ++ ++ VER+GQ +I
Sbjct: 210 SDSFYRVGLQDNDMAVALNGLDLRDAEQAKKAMERMADVHNFTLTVERDGQRQDI 264


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0151BCTERIALGSPD5940.0 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 594 bits (1534), Expect = 0.0
Identities = 327/681 (48%), Positives = 444/681 (65%), Gaps = 35/681 (5%)

Query: 6 IRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKI 65
IR + ++ A + +E+++A+FKGTDIQEFIN V KNLNKT+I+DP++RG I
Sbjct: 7 IRSFSLTLLIFAALLFRP--AAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTI 64

Query: 66 NVRSYDLLNDEQYYQFFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLG 125
VRSYD+LN+EQYYQFFL+VL VYG+A++ M N V+KV++ KDAKTAA+ VA+D PG+G
Sbjct: 65 TVRSYDMLNEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIG 124

Query: 126 DEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLSGRAAVVNKLVEIVR 185
DE+VTR+V L N A+ LAPLLRQLNDNAG G+VV+Y+PSNVL+++GRAAV+ +L+ IV
Sbjct: 125 DEVVTRVVPLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVE 184

Query: 186 RVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQAQLPGQAPKVVADERINAVVVSG 245
RVD GD SV VPL +ASA ++V+++ L + T+ A VVADER NAV+VSG
Sbjct: 185 RVDNAGDRSVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSG 244

Query: 246 DEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEGEKDPSAQAG 305
+ SRQR++ +I +LD +QA+ GNTKV YL+YAKA DLVEVLTG + ++ EK +
Sbjct: 245 EPNSRQRIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVA 304

Query: 306 GKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDNVG 365
+N I I AH TNAL+++A PD M +E VI QLDIRR QVLVEAII EV + D +
Sbjct: 305 ALDKN-IIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLN 363

Query: 366 FGVQWAAKADGGTQFNNLGPTIGEIGAGIWQAQDKEGTYITNPSTGEVIGKNPDTKGDVT 425
G+QWA K G TQF N G I AG + G V+
Sbjct: 364 LGIQWANKNAGMTQFTNSGLPISTAIAG---------------------ANQYNKDGTVS 402

Query: 426 -LLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVGDEVP 484
LA AL NG+A G G++ L+ A+S+ T +++LATPSI TLDN EA+F VG EVP
Sbjct: 403 SSLASALSSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVP 462

Query: 485 ILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNG-----NTG 539
+LTGS +++ N F TVERK VG+KLKV PQINEG++V L IEQEVS V ++
Sbjct: 463 VLTGSQ-TTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSD 521

Query: 540 VDISFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPFLGDIPVLGHLFKSSSSKKT 599
+ +F TR + V+ SG+ VV+GGL+++ V ++ KVP LGDIPV+G LF+S+S K +
Sbjct: 522 LGATFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVS 581

Query: 600 KKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQ--QERGVNLMPNTQVPILEEWNQ 657
K+NLM+FI+PT+IRD + +Y F Q +Q +E ++ + I Q
Sbjct: 582 KRNLMLFIRPTVIRDRDEYRQASSGQYTAFNDAQSKQRGKENNDAMLNQDLLEIYP--RQ 639

Query: 658 SEYLPPEVNDILDRYKEGKGL 678
+V+ +D + G L
Sbjct: 640 DTAAFRQVSAAIDAFNLGGNL 660


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0153BCTERIALGSPF5080.0 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 508 bits (1309), Expect = 0.0
Identities = 231/407 (56%), Positives = 307/407 (75%), Gaps = 1/407 (0%)

Query: 1 MPAFEYKALDAKGKQLKGVIEADTARHARSQLRDQRMMPLEILPVTEKEAKSKGTGFS-P 59
M + Y+ALDA+GK+ +G EAD+AR AR LR++ ++PL + + KS TG S
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 60 FKRGISVAELALITRQIATLVAAGLPIEECLKAVGQQCEKARLASMIMAVRSRVVEGYSL 119
K +S ++LAL+TRQ+ATLVAA +P+EE L AV +Q EK L+ ++ AVRS+V+EG+SL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 120 ADSLAEFPHIFDDLYRAMVASGEKSGHLEVVLNRLADYTERRQQLKSKLQQAMIYPIMLT 179
AD++ FP F+ LY AMVA+GE SGHL+ VLNRLADYTE+RQQ++S++QQAMIYP +LT
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 180 IVAIGVVSVLLAAVVPKVVGQFEHMGAELPATTRFLIAASDFVQHYGLFVILAIGILLVV 239
+VAI VVS+LL+ VVPKVV QF HM LP +TR L+ SD V+ +G +++LA+ +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 240 FQRLLKSPIFKMKYHTFLLKMPVIGRVSKGLNTARFARTLSILSASSVPLLDGMRIASEV 299
F+ +L+ ++ +H LL +P+IGR+++GLNTAR+ARTLSIL+AS+VPLL MRI+ +V
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 300 LQNVRVRAAVDDATARVREGTSLGAALTNTKLFPAMMLYMIASGEKSGQLEEMLERAADN 359
+ N R + AT VREG SL AL T LFP MM +MIASGE+SG+L+ MLERAADN
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 360 QDREFESNVTLALGVFEPMLVVSMAGVVLFIVMAILQPILALNNLIS 406
QDREF S +TLALG+FEP+LVVSMA VVLFIV+AILQPIL LN L+S
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLMS 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0154BCTERIALGSPG2317e-82 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 231 bits (591), Expect = 7e-82
Identities = 98/144 (68%), Positives = 119/144 (82%)

Query: 1 MQMNKKHKGFTLLEVMVVIVILGILASMVVPNLMGNKDKADQQKAVSDIVALENALDMYK 60
M+ K +GFTLLE+MVVIVI+G+LAS+VVPNLMGNK+KAD+QKAVSDIVALENALDMYK
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYK 60

Query: 61 LDNGVYPTTEQGLEALVQKPTISPEPRNYREEGYVKRLPQDPWRNNYLLLSPGENSKLDI 120
LDN YPTT QGLE+LV+ PT+ P NY +EGY+KRLP DPW N+Y+L++PGE+ D+
Sbjct: 61 LDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDL 120

Query: 121 FSAGPDGQPGTEDDIGNWNLQNFQ 144
SAGPDG+ GTEDDI NW L +
Sbjct: 121 LSAGPDGEMGTEDDITNWGLSKKK 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0155BCTERIALGSPH861e-23 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 86.2 bits (213), Expect = 1e-23
Identities = 43/171 (25%), Positives = 70/171 (40%), Gaps = 39/171 (22%)

Query: 4 LRHAGFTLMEVMLVILLMGLTAAAVTMSIGNSGSQQALEKNAQQFIAATELVLDETVLSG 63
+R GFTL+E+ML++LLMG++A V ++ S A + +F A V + +G
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQT-LARFEAQLRFVQQRGLQTG 59

Query: 64 QFIGIVVEKTSYQFVYYKDG---------------KWNPLEKDRILSEKQMEPGVVLNLV 108
QF G+ V +QF+ + +W PL R+ +
Sbjct: 60 QFFGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAGRVATSGS---------- 109

Query: 109 LDGLPLVQEDEKDESWFDEPLIEPSAEDKKKHPEPQILLFPSGEMSAFELS 159
+ G L + E+W P +L+FP GEM+ F L+
Sbjct: 110 IAGGKLNLAFAQGEAW-------------TPGDNPDVLIFPGGEMTPFRLT 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0156PilS_PF08805319e-04 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 30.7 bits (69), Expect = 9e-04
Identities = 11/32 (34%), Positives = 17/32 (53%)

Query: 5 KGMTLLEVIVALAVFAIAAVSITKSLGEQMAN 36
KG TL+EV++ + V + A S K +N
Sbjct: 26 KGATLMEVLLVVGVIVVLAASAYKLYSMVQSN 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0157BCTERIALGSPG310.002 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 31.4 bits (71), Expect = 0.002
Identities = 14/41 (34%), Positives = 26/41 (63%), Gaps = 3/41 (7%)

Query: 3 LKQTKAHKGFTLLEMLIAIAIFAMLGLAANAVLSTVLTNDE 43
++ T +GFTLLE+++ I I +G+ A+ V+ ++ N E
Sbjct: 1 MRATDKQRGFTLLEIMVVIVI---IGVLASLVVPNLMGNKE 38


57Shewana3_0249Shewana3_0262N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_02490152.940824short-chain dehydrogenase/reductase SDR
Shewana3_02500142.914021aldehyde dehydrogenase
Shewana3_02511162.660586Fis family GAF modulated sigma54 specific
Shewana3_02521121.311410ATPase domain-containing protein
Shewana3_0253-1161.954575two component transcriptional regulator
Shewana3_0254-1152.278951hypothetical protein
Shewana3_02550171.821273cation diffusion facilitator family transporter
Shewana3_0256-1171.491415hypothetical protein
Shewana3_0257-1171.392155outer membrane protein
Shewana3_0258-1201.639564nitrogen metabolism transcriptional regulator,
Shewana3_02591151.209478signal transduction histidine kinase, nitrogen
Shewana3_0260-115-0.035561hypothetical protein
Shewana3_0261-113-0.919798iron-containing alcohol dehydrogenase
Shewana3_0262015-1.422811TetR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0249DHBDHDRGNASE914e-24 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 91.3 bits (226), Expect = 4e-24
Identities = 71/257 (27%), Positives = 114/257 (44%), Gaps = 16/257 (6%)

Query: 6 IALITGASRGLGKNAALTLAAQGVDIILTYQSNAAAAAEVVAEIEWHGRKAVALPLDVGD 65
IA ITGA++G+G+ A TLA+QG I N +VV+ ++ R A A P DV D
Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHI-AAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 66 SQSFSDFSLRVKAALEQTWLRDSFNYLVNNAGIGIHVPMAETSVEQFDTLMNIHVKGPFF 125
S + + + R++ + + LVN AG+ + S E+++ +++ G F
Sbjct: 69 SAAIDEITARIEREMGP------IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122

Query: 126 LTQALLPLLAD--NGSIINVSTGLTRFAVPGFGAYAIMKGAVETMTKYWAKELGPRGIRV 183
++++ + D +GSI+ V + AYA K A TK EL IR
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 184 NVLAPGAIETDFGGGAVRDNPKMNEFLAQQTA-------LGRVGLPEDIGGAISVLLSPA 236
N+++PG+ ETD D + + L ++ P DI A+ L+S
Sbjct: 183 NIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 237 AAWINAQRIEASGGMFL 253
A I + GG L
Sbjct: 243 AGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0251HTHFIS341e-113 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 341 bits (876), Expect = e-113
Identities = 120/375 (32%), Positives = 200/375 (53%), Gaps = 17/375 (4%)

Query: 268 FHRDSALHVQTQALALTQTKSTRTLQDKPLNQLGVRFRDPLLERAWQQANKVITKQIPLL 327
F + + +ALA + + ++ D + R ++ ++ +++ + L+
Sbjct: 106 FDLTELIGIIGRALAEPKRRPSKLEDDSQDGM-PLVGRSAAMQEIYRVLARLMQTDLTLM 164

Query: 328 VLGETGVGKEQFVKKLHAQSTRRAQPIVAVNCAALPAELVESELFGYQAGAFTGANRTGF 387
+ GE+G GKE + LH RR P VA+N AA+P +L+ESELFG++ GAFTGA
Sbjct: 165 ITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRS- 223

Query: 388 IGKIRQAHGGFLFLDEIGEMPLAAQSRLLRVLQEREVVPVGSNQSFKVDIQIIAATHMDL 447
G+ QA GG LFLDEIG+MP+ AQ+RLLRVLQ+ E VG + D++I+AAT+ DL
Sbjct: 224 TGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDL 283

Query: 448 ESLVAQGLFRQDLFYRLNGLQVRLPALRERQ-DIERIIH---KLHRRHRSSAQTLCTELL 503
+ + QGLFR+DL+YRLN + +RLP LR+R DI ++ + + + E L
Sbjct: 284 KQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEAL 343

Query: 504 AQLMRYDWPGNLRELDNLMQVACLMAEGEAVLEINHLPDYLAQKLMNLACEPQTLTEVAD 563
+ + WPGN+REL+NL++ + + V+ + + L ++ + E +
Sbjct: 344 ELMKAHPWPGNVRELENLVRRLTALYPQD-VITREIIENELRSEIPDSPIEKAAARSGSL 402

Query: 564 AEATAHPHELVESSSVTIDSLHGTINLN----------VLQAYRACDGNVSQCAKRLGIS 613
+ + A + + + D+L + + +L A A GN + A LG++
Sbjct: 403 SISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLN 462

Query: 614 RNALYRKLKQLGIKD 628
RN L +K+++LG+
Sbjct: 463 RNTLRKKIRELGVSV 477


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0252PF06580371e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 37.2 bits (86), Expect = 1e-04
Identities = 25/121 (20%), Positives = 51/121 (42%), Gaps = 12/121 (9%)

Query: 274 IAYEAEQLEKLIAELLELSRVKLSTNETKVRLGLAESLSQVLDDAEFEADQQGKKIT--I 331
I + + +++ L EL R L + + + LA+ L+ V + + Q ++
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYSNARQ-VSLADELTVVDSYLQLASIQFEDRLQFEN 244

Query: 332 DIDEAIELSHYPKSLSRAIENLLRNAIRYA------QSDIHLLASQANGQVQITIKDDGP 385
I+ AI P L ++ L+ N I++ I L ++ NG V + +++ G
Sbjct: 245 QINPAIMDVQVPPML---VQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGS 301

Query: 386 G 386

Sbjct: 302 L 302


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0253HTHFIS972e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 96.8 bits (241), Expect = 2e-25
Identities = 46/163 (28%), Positives = 77/163 (47%), Gaps = 3/163 (1%)

Query: 2 SRILLIDDDLGLSELLGQLLELEGFKLTLAYDGKQGLELALAGDYDLILLDVMLPKLNGF 61
+ IL+ DDD + +L Q L G+ + + + AGD DL++ DV++P N F
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 EVLRALRQH-KQTPVLMLTARGDEIDRVVGLEIGADDYLPKPFNDRELIARIRAIIRRSH 120
++L +++ PVL+++A+ + + E GA DYLPKPF+ ELI I +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 121 LTAQEIHATPAQEFGDLRLDPSRQEAYCNEQLIILTGTEFTLL 163
++ + + QE Y L L T+ TL+
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIY--RVLARLMQTDLTLM 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0256PF01206270.007 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 27.4 bits (61), Expect = 0.007
Identities = 5/35 (14%), Positives = 14/35 (40%)

Query: 90 PLLMWRSRVTCEQSGKVVIIECLDERKRRSIIRWC 124
P+L + + +G+V+ + D + +
Sbjct: 18 PILKAKKTLATMNAGEVLYVMATDPGSVKDFESFS 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0257OMPADOMAIN571e-12 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 57.2 bits (138), Expect = 1e-12
Identities = 27/122 (22%), Positives = 40/122 (32%), Gaps = 12/122 (9%)

Query: 1 MKKLSLVAVSLLSALVAGQASAAADTTGFYVGGAL-------NRVAVDVVDDSDTGTGFG 53
MKK +A+++ A A A AA +Y G L + + G G
Sbjct: 1 MKKT-AIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTHENQLGAG 59

Query: 54 VYGGYNFNEWFGLEANLF----ATADLGDRDTDVTAGALTFTPKFTLQINDMFSAYAKVG 109
+GGY N + G E + A + T K I D Y ++G
Sbjct: 60 AFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLG 119

Query: 110 VA 111

Sbjct: 120 GM 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0258HTHFIS5570.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 557 bits (1438), Expect = 0.0
Identities = 198/474 (41%), Positives = 294/474 (62%), Gaps = 12/474 (2%)

Query: 5 VWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTDGLTL 64
+ + DDD++IR VL +AL A + A +LW+ + ++V+D+ MP + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 65 LERLQIHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQ 124
L R++ P +PV++M+A + +A+ A + GA++YLPKPFD+ E I ++ RAL +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE--PK 123

Query: 125 SPAPAAQETQVKTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAGALHKH 184
++ ++G + AMQE++R + RL ++ ++++I G+SGTGKELVA ALH +
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY 183

Query: 185 SPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDEIGDM 244
R++ PF+A+NMAAIP+DLIESELFGHEKGAFTGA GRFEQA GGTLFLDEIGDM
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 245 PLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHRLNVI 304
P+D QTRLLRVL G++ VGG + ++ DVRI+AAT++DL+Q + +G FREDL++RLNV+
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 305 RVHLPPLSQRREDIPQLATHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQLENTC 364
+ LPPL R EDIP L HF+ A KE G++ K +E + PWPGNVR+LEN
Sbjct: 304 PLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 365 RWLTVMASGQEILPQDLPQELLKEPASINPMAKGSQDWQSALTEWIDQKLSE-------- 416
R LT + I + + EL E ++ ++++ +++ + +
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 417 -GNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD 469
S L V E L+ AL T+G++ +AA LG RNTL +K++EL +
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0259PF06580406e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 40.2 bits (94), Expect = 6e-06
Identities = 35/187 (18%), Positives = 72/187 (38%), Gaps = 33/187 (17%)

Query: 167 LIIEQADRLRSLVDRL-------LGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKR 219
LI+E + R ++ L L Q SL + VV +L + +Q +
Sbjct: 185 LILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFEN 244

Query: 220 DYDPSIPDIEMDPDQMQQAVLNILQNAVQALEHTGGEILIRTRTQHQVTIGSQRHKLVLT 279
+P+I D+++ P +Q V N +++ + L GG+IL++ + +T
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQ-GGKILLKGTKDNGT----------VT 293

Query: 280 LSIIDNGPGIPPELMDTLFYPMVTSREQGSGLGLSIAHNIARLHSG---RIDCVSSPGHT 336
L + + G + + ++ +G GL ++ G +I G
Sbjct: 294 LEVENTGSL------------ALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKV 341

Query: 337 EFIISLP 343
++ +P
Sbjct: 342 NAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0262HTHTETR602e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 59.6 bits (144), Expect = 2e-13
Identities = 25/77 (32%), Positives = 33/77 (42%)

Query: 1 MKTETQSTRQHILDVGYSLILKQGFSCLGLAQLLKAAQVPKGSFYHYFKSKEQFGEALLA 60
K E Q TRQHILDV L +QG S L ++ KAA V +G+ Y +FK K +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 61 GYFEQYQAELDSLLNET 77
+
Sbjct: 65 LSESNIGELELEYQAKF 81


58Shewana3_0371Shewana3_0378N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0371-310-1.452372acriflavin resistance protein
Shewana3_0372-210-1.806733RND family efflux transporter MFP subunit
Shewana3_0373-210-1.626216TetR family transcriptional regulator
Shewana3_0374-29-1.509570ATP-dependent DNA helicase Rep
Shewana3_0375-111-2.809656GAF sensor-containing diguanylate
Shewana3_0376017-3.652749****diguanylate cyclase/phosphodiesterase
Shewana3_037710234.335831hypothetical protein
Shewana3_037810224.276818OmpA/MotB domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0371ACRIFLAVINRP7870.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 787 bits (2033), Expect = 0.0
Identities = 322/1038 (31%), Positives = 538/1038 (51%), Gaps = 36/1038 (3%)

Query: 5 DIFIRRPVLAASISFLLLLLGFNALNSMQVREYPKMTNTVVTVSTSYYGADANLIQGFIT 64
+ FIRRP+ A ++ +L++ G A+ + V +YP + V+VS +Y GADA +Q +T
Sbjct: 3 NFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVT 62

Query: 65 QPLEQALAQADNVDFMTSESF-LGTSKISVYMKLNTDPNGALADILAKVNSVRSQLPKEA 123
Q +EQ + DN+ +M+S S G+ I++ + TDP+ A + K+ LP+E
Sbjct: 63 QVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEV 122

Query: 124 EDPSVEMSTGSQTSVLYISFFSDQINSSQ--LTDYLERVVKPQLFTIDGVAKVNLYGGIK 181
+ + + S + ++ F SD ++Q ++DY+ VK L ++GV V L+G +
Sbjct: 123 QQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA-Q 181

Query: 182 YAMRIWLDPARMGAFKLSSSDVMQVLQANNYQSAVGQTNGVYTL------FNGTADTQVA 235
YAMRIWLD + +KL+ DV+ L+ N Q A GQ G L + A T+
Sbjct: 182 YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 236 TIEELKRLVI-GSKDGLVVRLGDIADVSLEKSHDIYRALANGKEAVVIGLDVTPTANPLN 294
EE ++ + + DG VVRL D+A V L + A NGK A +G+ + AN L+
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 295 VAADTRALLPEIERNLPPSIGSSILYDSSLAIDESIKEVVKTIGEAAIIVIVVITLFLGS 354
A +A L E++ P + YD++ + SI EVVKT+ EA ++V +V+ LFL +
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 355 LRAVVIPIVTIPLSLIGVAIIMQMFGFTLNLMTLLAMVLAIGLVVDDAIVVVENVDRHIK 414
+RA +IP + +P+ L+G I+ FG+++N +T+ MVLAIGL+VDDAIVVVENV+R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 LGESPFRAAII-GTREIAVPVISMTITLAAVYAPIALMGGITGSLFKEFALTLAGSVFIS 473
+ P + A +I ++ + + L+AV+ P+A GG TG+++++F++T+ ++ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 474 GIVALTLSPMMCAKILKP-----HTTPNRFEMGVENFLTGLTRRYSNMLDAVMLHRPVIV 528
+VAL L+P +CA +LKP H F Y+N + ++ +
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 529 AFAIIVFASLPVLFKFIPSELAPNEDKGVVMMMGTAPSTANLDYIQANMGLVTDMIKAQP 588
++ A + VLF +PS P ED+GV + M P+ A + Q + VTD
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 589 ESAASLAF----VGVPSSSQAFGIA--PLVPWSER----EKSQKQMQEFFAKEVKHIPGM 638
++ F +Q G+A L PW ER ++ + E+ I
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAK-MELGKIRDG 660

Query: 639 AITTFQMPE--LPGASSGLPIQFVITTSNSFASLFQIGTGVLEKVQKSPLFVYSEI-NLK 695
+ F MP G ++G + + +L Q +L + P + S N
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 696 FDSGTMKLHIKRDLAGTYGVTMQDIGITLATMMSDGYVNRINLDGRSYEVIPQVERKLRA 755
D+ KL + ++ A GV++ DI T++T + YVN GR ++ Q + K R
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 756 NPESLANYYVKAADGKSIPLSSLVDIEMVAEPRSLPHFNQMNALTVGGVAAPGVAIGDAI 815
PE + YV++A+G+ +P S+ V L +N + ++ + G AAPG + GDA+
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 816 NFLQNIGDNELPKGYSYDFLGEARQFVTEGSALYATFLLAIAIIFLVLASQFESLKDPLV 875
++N+ ++LP G YD+ G + Q G+ A ++ ++FL LA+ +ES P+
Sbjct: 841 ALMENL-ASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 876 ILVSVPLAISGALIVLGWTHVFGLAKINIYTQVGLITLVGLITKHGILMCEVAKEEQLHR 935
+++ VPL I G L+ + K ++Y VGL+T +GL K+ IL+ E AK+
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQ----KNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKE 955

Query: 936 GLSKLEAIKLAATIRLRPILMTTAAMIAGLLPLLFASGAGAVARFNIGIVIVAGLSIGTI 995
G +EA +A +RLRPILMT+ A I G+LPL ++GAG+ A+ +GI ++ G+ T+
Sbjct: 956 GKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATL 1015

Query: 996 FTLFVLPVIYTYLAEKHE 1013
+F +PV + + +
Sbjct: 1016 LAIFFVPVFFVVIRRCFK 1033


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0372RTXTOXIND445e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.4 bits (105), Expect = 5e-07
Identities = 23/130 (17%), Positives = 33/130 (25%), Gaps = 35/130 (26%)

Query: 71 TISNELAGRVTSINFENGSRVEKGQLLAELDAKVERANLKSKMVQLPAAEADFKRLSKLY 130
I V I + G V KG +L +L A A+ L A + R L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 131 -----------------------------------AQKSVSKQDLDNSESKYLALQADIE 155
Q S + E +A+
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 156 SLKATIERRE 165
++ A I R E
Sbjct: 218 TVLARINRYE 227



Score = 43.7 bits (103), Expect = 8e-07
Identities = 37/253 (14%), Positives = 75/253 (29%), Gaps = 71/253 (28%)

Query: 88 GSRVEKGQLLAELDAKVERANLKSKMVQLPAAEADFKRLSKLYAQKSVSKQDLDNSESKY 147
+ + AE + R N ++ S L +++++K + E+KY
Sbjct: 204 QKELNLDKKRAERLTVLARINRYEN--LSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKY 261

Query: 148 LALQADIESLKATIER-------------------------------------------- 163
+ ++ K+ +E+
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKN 321

Query: 164 ------REISAPFSGLVGIRNIN-LGEYLQPGT---DIVRLEDISTMKIRFTIPQTQLPR 213
I AP S V ++ G + IV +D T+++ + +
Sbjct: 322 EERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDD--TLEVTALVQNKDIGF 379

Query: 214 IAVGQKIHVFVDSYPEQ---PFEGEIAAIEP--------AVFYQSGLIQVQARIP--NDN 260
I VGQ + V+++P G++ I + + + + + N N
Sbjct: 380 INVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNKN 439

Query: 261 AKLRSGMFARVSI 273
L SGM I
Sbjct: 440 IPLSSGMAVTAEI 452


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0373HTHTETR617e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 60.8 bits (147), Expect = 7e-14
Identities = 34/136 (25%), Positives = 56/136 (41%), Gaps = 6/136 (4%)

Query: 2 DSKRDLILRSAEKIIATEGLHNLSMQKLAADAGVAAGTIYRYFKDKEDLIIELRKDVLQQ 61
R IL A ++ + +G+ + S+ ++A AGV G IY +FKDK DL E+ +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 62 IASKLLENINE--GSFDQKFRRLWFNIVALGRKQSHANLSFAQYSHL---PGVDAPEHQA 116
I LE + G R + +++ + L H G A QA
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 117 FEREIFQQLHQLFEQA 132
+R + + + EQ
Sbjct: 130 -QRNLCLESYDRIEQT 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0378OMPADOMAIN885e-23 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 88.1 bits (218), Expect = 5e-23
Identities = 33/118 (27%), Positives = 53/118 (44%), Gaps = 12/118 (10%)

Query: 77 NVLFPNDSAYIAPEYYPQIEEIAMFLRQY--PTTKVTIEGHTSRTGTDERNAVLSQERAD 134
+VLF + A + PE ++++ L V + G+T R G+D N LS+ RA
Sbjct: 220 DVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQ 279

Query: 135 AVTAVLADRFSIDRSRLTAIGYGSSRPLVLEHTPDAETR---------NRRVVAEVTG 183
+V L + I +++A G G S P+ + + R +RRV EV G
Sbjct: 280 SVVDYLISK-GIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKG 336


59Shewana3_0502Shewana3_0510N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_05020120.639365peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_05030110.258027flavoprotein oxygenase
Shewana3_05040120.254928hypothetical protein
Shewana3_05051143.829455FMN reductase
Shewana3_05061153.9239933-octaprenyl-4-hydroxybenzoate carboxy-lyase
Shewana3_05073203.934603hypothetical protein
Shewana3_05083193.845947major facilitator superfamily transporter
Shewana3_05091172.396309short-chain dehydrogenase/reductase SDR
Shewana3_05103171.813010biotin carboxyl carrier protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0502SUBTILISIN1096e-28 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 109 bits (275), Expect = 6e-28
Identities = 59/269 (21%), Positives = 87/269 (32%), Gaps = 88/269 (32%)

Query: 210 TDRGPEFIGADQMWQGTATQGGLPVKGEGMVVGIIDTGINTDHVAFADDEEYARLNPYKG 269
RG E I A +W T +G G+ V ++DTG + DH
Sbjct: 22 IPRGVEMIQAPAVWNQT--------RGRGVKVAVLDTGCDADHPDLKA------------ 61

Query: 270 QAIGDCGAFPELCNNKLVGLHSYPEITDVYAAPEFQTSSGAKKRIRPANAEDYAGHGSHT 329
+++G ++ + + P +DY GHG+H
Sbjct: 62 ---------------RIIGGRNFTDDDE----------------GDPEIFKDYNGHGTHV 90

Query: 330 ASTVAGNTLKDTPLQGFTGDKVSDGVDVPFTFPQTSGVAPRAHIIAYQVCWPGTSGDPYA 389
A T+A ++ GVAP A ++ +V SG
Sbjct: 91 AGTIAAT-----------ENENG-----------VVGVAPEADLLIIKVLNKQGSGQ--- 125

Query: 390 GCPESAILSAFEDAIADGVDAINFSIGGAENMPWGDPMELAFLSAREAGISVAAAAGNSG 449
I+ AI VD I+ S+GG E+ + A A + I V AAGN G
Sbjct: 126 ---YDWIIQGIYYAIEQKVDIISMSLGGPED---VPELHEAVKKAVASQILVMCAAGNEG 179

Query: 450 AYWTADH------SSPWVTTVGATTHDRK 472
V +VGA DR
Sbjct: 180 DGDDRTDELGYPGCYNEVISVGAINFDRH 208



Score = 72.6 bits (178), Expect = 2e-15
Identities = 32/131 (24%), Positives = 48/131 (36%), Gaps = 32/131 (24%)

Query: 627 NNLATFSSLGPSKTNNTLVPDLTAPGVDIYAANADDQPFTNNPSASDWTFMSGTSMAAPH 686
+ + FS+ DL APG DI + + SGTSMA PH
Sbjct: 207 RHASEFSNSNNE-------VDLVAPGEDILSTVPG----------GKYATFSGTSMATPH 249

Query: 687 VTGAMTLLTQL-----HPDWTPAEIQSALMLTAGPVVLNTGYELVEPYYNFMAGAGAINV 741
V GA+ L+ QL D T E+ + L+ P+ + E G G + +
Sbjct: 250 VAGALALIKQLANASFERDLTEPELYAQLIKRTIPLGNSPKME----------GNGLLYL 299

Query: 742 ARAADTGLVMD 752
+ + D
Sbjct: 300 TAVEELSRIFD 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0507IGASERPTASE345e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.3 bits (78), Expect = 5e-04
Identities = 21/80 (26%), Positives = 31/80 (38%), Gaps = 2/80 (2%)

Query: 145 PMAYDDTPVAVSPPVRVTTSMQYSPSEGRMVSNMPTNSATVISQTGVSTTRASTASAEQM 204
P + +T P + T+S P N NS + T T ++E
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVN-TGNSVVENPENTTPATTQPTVNSE-S 1215

Query: 205 ANVPRARAARSVSSLPSNAR 224
+N P+ R RSV S+P N
Sbjct: 1216 SNKPKNRHRRSVRSVPHNVE 1235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0508TCRTETA514e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 51.0 bits (122), Expect = 4e-09
Identities = 60/322 (18%), Positives = 106/322 (32%), Gaps = 22/322 (6%)

Query: 52 VAHVSYAISAYALGVVVGSPIIMVLGVRIKRRTLLIALAAMMAVANGLSALAPSLNWLVF 111
AH ++ YAL +P++ L R RR +L+ A AV + A AP L L
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYI 101

Query: 112 FRFLSGLPHGAYFGVAMLLAASLVPPEMKARAVSRVIIGLTLATIVGVPFATWMGQTVGW 171
R ++G+ GA VA A + + +AR + + G MG
Sbjct: 102 GRIVAGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSP 159

Query: 172 RSGIGIVAIIAAVTAVMLYFLAPNVAVPQNASPKKELQTLKNREVWLTLGIAAIGFGGIF 231
+ A + + + FL P + + N +
Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPE---SHKGERRPLRREALNPLASFRWARGMTVVAALM 216

Query: 232 CVYTYLAETLIQVTQV------------EPFKIPVMMAVFGI-GATLGTLVCGWAADK-S 277
V+ ++ + + QV + I + +A FGI + ++ G A +
Sbjct: 217 AVF-FIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLG 275

Query: 278 ALAAAFWSLVLSTLVLALYPSLTGSYWALMPI-VFFVGSGIGLATTVQARLMDVAPDGQA 336
A ++ L + W PI V GIG+ V + Q
Sbjct: 276 ERRALMLGMIADGTGYILL-AFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG 334

Query: 337 MTGALVQCAFNLANAIGPWVGS 358
+ +L + +GP + +
Sbjct: 335 QLQGSLAALTSLTSIVGPLLFT 356


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0509DHBDHDRGNASE547e-11 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 53.9 bits (129), Expect = 7e-11
Identities = 41/183 (22%), Positives = 80/183 (43%), Gaps = 5/183 (2%)

Query: 2 ILITGASSGLGAALAALYAKDNQALTLTGRDANRLHTVANALSPFSTQSISAISADLADE 61
ITGA+ G+G A+A A + + +L V ++L + + A AD+ D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHA-EAFPADVRDS 69

Query: 62 ASVEALFDGL---TDTPNTVIHCAGSGYFGTLETQGTSEIQALLNNNVTSTILLVRELVK 118
A+++ + + + +++ AG G + + E +A + N T R + K
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129

Query: 119 RYK-QQAVKVVIVMSTAALTAKAGESTYCAAKWAVRGFIESVRLELKQSPMKLIAVYPGG 177
+++ +V V S A + + Y ++K A F + + LEL + ++ V PG
Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189

Query: 178 MDT 180
+T
Sbjct: 190 TET 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0510RTXTOXIND310.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.002
Identities = 10/32 (31%), Positives = 14/32 (43%)

Query: 120 IEAKRDGIVGAIWVKDGDEVAFDQPLFTLIET 151
I+ + IV I VK+G+ V L L
Sbjct: 99 IKPIENSIVKEIIVKEGESVRKGDVLLKLTAL 130


60Shewana3_0516Shewana3_0526N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0516-1184.158070outer membrane efflux family protein
Shewana3_0517-2174.086235RND family efflux transporter MFP subunit
Shewana3_0518-2183.584552CzcA family heavy metal efflux protein
Shewana3_0519-1151.457991antibiotic biosynthesis monooxygenase
Shewana3_0520-1161.550805large-conductance mechanosensitive channel
Shewana3_0521-2161.493397LysR family transcriptional regulator
Shewana3_0522-2151.065338secretion protein HlyD family protein
Shewana3_0523-1130.314032EmrB/QacA family drug resistance transporter
Shewana3_0524019-2.451085N-acetyltransferase GCN5
Shewana3_0525015-2.257181antibiotic biosynthesis monooxygenase
Shewana3_0526117-2.797947sigma-54 dependent trancsriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0516RTXTOXIND330.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.9 bits (75), Expect = 0.002
Identities = 16/154 (10%), Positives = 45/154 (29%), Gaps = 12/154 (7%)

Query: 76 EVQAQIARQQQAELAIAAADRAIYNPEL-GLNYQNADTDTYSLGLSQTLDWGDKRGVATR 134
+ + QA L + EL L + Y +S+ + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 135 LAQLEAQILLADIQLERSQMLAERLLALAEQAQSNKALTFAEQQLRFTQAQLNIAEQRFA 194
+ + Q ++ L++ + AE+ + E R +++L+
Sbjct: 195 FSTWQNQKYQKELNLDKKR---------AERLTVLARINRYENLSRVEKSRLDDFSSLLH 245

Query: 195 AGDLSDVELQLLKLELASNTADYALAEQAALVAD 228
++ + + + + L + +
Sbjct: 246 KQAIAKHAVLEQENKYVEAVNE--LRVYKSQLEQ 277


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0517RTXTOXIND561e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 56.0 bits (135), Expect = 1e-10
Identities = 31/138 (22%), Positives = 55/138 (39%), Gaps = 9/138 (6%)

Query: 156 EVAKAQAEYINAAAEWSRVRR---MSEGAVSVSRRMQAQVDAELKRAILEAIKMTDAQIR 212
V + + +Y+ A E + E + ++ V K IL+ ++ T I
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 213 TLE----STPEAIGSYQLLAPIDGRVQQ-DIAMLGQVFTAGTPLMQLT-DESHLWVEAQL 266
L E + + AP+ +VQQ + G V T LM + ++ L V A +
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 267 TPAQAANVNVGGPALVQV 284
+NVG A+++V
Sbjct: 373 QNKDIGFINVGQNAIIKV 390



Score = 42.9 bits (101), Expect = 2e-06
Identities = 26/149 (17%), Positives = 54/149 (36%), Gaps = 5/149 (3%)

Query: 100 SFSNLNLDTMATATLVVDRDRTATLAPQLDVRVQARHVVPGQEVKKGEPLLTLGG----A 155
+ + A L R+ + P + V+ V G+ V+KG+ LL L A
Sbjct: 76 VLGQVEIVATANGKLTHS-GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 156 EVAKAQAEYINAAAEWSRVRRMSEGAVSVSRRMQAQVDAELKRAILEAIKMTDAQIRTLE 215
+ K Q+ + A E +R + +S D + + E + + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 216 STPEAIGSYQLLAPIDGRVQQDIAMLGQV 244
+ YQ +D + + + +L ++
Sbjct: 195 FSTWQNQKYQKELNLDKKRAERLTVLARI 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0518ACRIFLAVINRP6540.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 654 bits (1690), Expect = 0.0
Identities = 224/1077 (20%), Positives = 438/1077 (40%), Gaps = 72/1077 (6%)

Query: 9 AIKNRLLVVLALLAVVAASVAMLPKLNLDAFPDVTNVQVTINTAAEGLAAEEVEKLISYP 68
I+ + + + ++ A + +L + +P + V+++ G A+ V+ ++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 69 VESAMYALPAVTEVRSLS-RTGLSIVTVVFAEGTDIYFARQQVFEQLQAAREMIPSGVGV 127
+E M + + + S S G +T+ F GTD A+ QV +LQ A ++P V
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 128 PEIGPNTSGLGQIYQYILRADPSSGINAAELRSLNDYLVKLIMMPVGGVTDVLSFGGEVR 187
I S + +D + G ++ VK + + GV DV FG +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSD-NPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ-Y 182

Query: 188 QYQVQVDPNKLRAYGLSMAQVSEALESNNRNAGGWFMDQGQEQLVVRGYGMLPAGEAGLA 247
++ +D + L Y L+ V L+ N + G L + +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLG-GTPALPGQQLNASIIAQTRFK 241

Query: 248 AIAQIPLTEVR----GTPVRVGDIAQVDFGSEIRVGAVTMTRRDEAGQAQDLGEVVAGVV 303
+ +R G+ VR+ D+A+V+ G E + G+ +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARIN-----GK-----PAAGLGI 291

Query: 304 LKRMGANTKATIDDIDARINLIEQALPKGVSFEVFYDQADLVDKAVTTVRDALLMAFVFI 363
GAN T I A++ ++ P+G+ YD V ++ V L A + +
Sbjct: 292 KLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLV 351

Query: 364 VVILALFLVNIRATLLVLLSIPVSIGLALMVMSYYGLSANLMSLGGLAVAIGMLVDGSVV 423
+++ LFL N+RATL+ +++PV + +++ +G S N +++ G+ +AIG+LVD ++V
Sbjct: 352 FLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIV 411

Query: 424 MVENIFKHLTQPDRRHLAQARSRADGEVDPYHGDEDGSAHAVEADNNMAVRIMLAAKEVC 483
+VEN+ R + ++ P + ++
Sbjct: 412 VVENV--------------ERVMMEDKLPPKEA------------------TEKSMSQIQ 439

Query: 484 SPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMISALLVALIAVPALAVYLFK- 542
+ ++ VF P+ G G +++ +++I+ AM ++LVALI PAL L K
Sbjct: 440 GALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKP 499

Query: 543 ---------RGVVLKESVVLAPLDSAYRKLLSATLARPKLVMTSALLMFAMSMVLLPRLG 593
G + + Y + L + L+ A +VL RL
Sbjct: 500 VSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLP 559

Query: 594 TEFVPELEEGTINLRVTLAPTASLGTSLDVAPKLEAMLLEFPEVEYALSRIGAPELGGDP 653
+ F+PE ++G + L A+ + V ++ L+ + S
Sbjct: 560 SSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVE-SVFTVNGFSFSG 618

Query: 654 EPVSNIEVYIGLKPIEEWQSASSRLE--LQRLMEEKLSVFPGLLLTFSQPIATRVDELLS 711
+ + ++ LKP EE + E + R E + G ++ F+ P + EL +
Sbjct: 619 QAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMP---AIVELGT 675

Query: 712 GVKAQLA-IKLFGPDLDVLSEKGQVLTDLVAKIPGAV-DVSLEQVSGEAQLVVRPDRAQL 769
I G D L++ L + A+ P ++ V + AQ + D+ +
Sbjct: 676 ATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKA 735

Query: 770 ARYGISVDQVMSLVSQGIGGASAGQVIDGNARYDINLRLAAQYRSSPDVIKDLLLSGSNG 829
G+S+ + +S +GG ID + ++ A++R P+ + L + +NG
Sbjct: 736 QALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANG 795

Query: 830 ATVRLGEVASVEVEMAPPNIRRDDVQRRVVVQANVA-GRDMGSVVKDIYALVPQADLPAG 888
V + P + R + + +Q A G G + + L + LPAG
Sbjct: 796 EMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASK--LPAG 853

Query: 889 YTVIVGGQYENQQRAQQKLMLVVPISIALIALLLYFSFGAVKQVLLIMANVPLALIGGIV 948
G ++ + + +V IS ++ L L + + + +M VPL ++G ++
Sbjct: 854 IGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLL 913

Query: 949 ALFVSGTYLSVPSSIGFITLFGVAVLNGVVLVDSINQ-RRQSGESLYDSVYEGTVGRLRP 1007
A + V +G +T G++ N +++V+ + G+ + ++ RLRP
Sbjct: 914 AATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRP 973

Query: 1008 VLMTALTSALGLIPILVSSGVGSEIQKPLAVVIIGGLFSSTALTLLVLPTLYRWLYR 1064
+LMT+L LG++P+ +S+G GS Q + + ++GG+ S+T L + +P + + R
Sbjct: 974 ILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR 1030



Score = 106 bits (266), Expect = 2e-25
Identities = 85/550 (15%), Positives = 186/550 (33%), Gaps = 68/550 (12%)

Query: 10 IKNRLLVVLALLAVVAASVAMLPKLNLDAFPDVTNVQVTIN-TAAEGLAAEEVEKLI--- 65
+ + +L +VA V + +L P+ G E +K++
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 66 -SYPVESAMYALPAVTEVRSLSRTGLS----IVTVVFAEGTDIYFARQQVFEQLQAAREM 120
Y +++ + +V V S +G + + V + + A+
Sbjct: 594 TDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKME 653

Query: 121 ---IPSGVGVPEIGPNTSGLGQIYQYILRADPSSGINAAELRSLNDYLVKLIMMPVGGVT 177
I G +P P LG + +G+ L + L+ + +
Sbjct: 654 LGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLV 713

Query: 178 DVLSFGGEVR-QYQVQVDPNKLRAYGLSMAQVSEALES--NNRNAGGWFMDQGQEQLVVR 234
V G E Q++++VD K +A G+S++ +++ + + + ++L V+
Sbjct: 714 SVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQ 773

Query: 235 GYGMLPAGEA-GLAAIAQIPLTEVRGTPVRVGDIAQVDFGSEIRVGAVTMTRRDEAGQAQ 293
A + ++ + G V + G+ + R + +
Sbjct: 774 ----ADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVY----GSPRLERYNGLPSME 825

Query: 294 DLGEVVAGVVLKRMGANTKATIDDIDARINLIEQALPKGVSFEVFYDQADLVDKAVTTVR 353
GE G D A + + LP G+ ++ + + +
Sbjct: 826 IQGEAAPGTSS-----------GDAMALMENLASKLPAGIGYD-WTGMSYQERLSGNQAP 873

Query: 354 DALLMAFVFIVVILALFLVNIRATLLVLLSIPVSIGLALMVMSYYGLSANLMSLGGLAVA 413
+ ++FV + + LA + + V+L +P+ I L+ + + ++ + GL
Sbjct: 874 ALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTT 933

Query: 414 IGMLVDGSVVMVENIFKHLTQPDRRHLAQARSRADGEVDPYHGDEDGSAHAVEADNNMAV 473
IG+ ++++VE A+ +G+ VEA
Sbjct: 934 IGLSAKNAILIVE-------------FAKDLMEKEGK------------GVVEA------ 962

Query: 474 RIMLAAKEVCSPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMISALLVALIAV 533
++A + PI + I+ PL G + + ++ M+SA L+A+ V
Sbjct: 963 -TLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFV 1021

Query: 534 PALAVYLFKR 543
P V + +
Sbjct: 1022 PVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0520MECHCHANNEL1742e-59 Bacterial mechano-sensitive ion channel signature.
		>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature.

Length = 136

Score = 174 bits (442), Expect = 2e-59
Identities = 88/136 (64%), Positives = 111/136 (81%), Gaps = 1/136 (0%)

Query: 1 MSLIKEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSIV 60
MS+IKEF+ FA RGNV+D+AVG+IIGAAFGKIVSS VADIIMPP+G+++GG++F ++
Sbjct: 1 MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVT 60

Query: 61 LQAAQGDAPAVVIAYGKFIQTIIDFTIIAFAIFMGLKAINSLKRKQEEAPKAPPAPTKDQ 120
L+ AQGD PAVV+ YG FIQ + DF I+AFAIFM +K IN L RK+EE P A PAPTK++
Sbjct: 61 LRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEE-PAAAPAPTKEE 119

Query: 121 ELLSEIRDLLKAQQEK 136
LL+EIRDLLK Q +
Sbjct: 120 VLLTEIRDLLKEQNNR 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0522RTXTOXIND936e-23 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 93.0 bits (231), Expect = 6e-23
Identities = 39/290 (13%), Positives = 91/290 (31%), Gaps = 28/290 (9%)

Query: 71 LAQLEDNQFSAKVSQAEALLASAKADMQTLAAKVELQHALISQASAGVVAAQADKLRAEQ 130
+ + + S + ++ + ++ + A A + + +
Sbjct: 176 FQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKS 235

Query: 131 QLSRAKKLKVSNYSSQDDVDQLQAGFDSAAAGLDEAKA--------LLVAKERELAVFN- 181
+L L ++ V + + + A L K+ +L AKE V
Sbjct: 236 RLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQL 295

Query: 182 ------AQLNQAGSVVEQSNAALELAKIQLNDTRVTAPFSGVIGKRGAM-VGQYVQPGQA 234
+L Q + L + + + + AP S + + G V +
Sbjct: 296 FKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAET 355

Query: 235 LYSLVPDGAV-WITANFKETQIQHMQPGQSVQVSLDAFPDKTFTGVIDSLSPASGAKFSL 293
L +VP+ +TA + I + GQ+ + ++AFP G + K
Sbjct: 356 LMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTR-YGYLV-------GKVKN 407

Query: 294 LPAENATGNFTKIVQRIPVRIRLDLSAE---EARVVPGLSAVVKVDTASH 340
+ + +V + + I + + + G++ ++ T
Sbjct: 408 INLDAIEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMR 457



Score = 56.0 bits (135), Expect = 8e-11
Identities = 23/128 (17%), Positives = 47/128 (36%), Gaps = 2/128 (1%)

Query: 59 VTDNQHVRKGELLAQLEDNQFSAKVSQAEALLASAKADMQTLAAKVELQHALISQASAGV 118
V + + VRKG++L +L A + ++ L A+ + L +
Sbjct: 112 VKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSR-SIELNKLPELKL 170

Query: 119 VAAQADKLRAEQQLSRAKKLKVSNYSS-QDDVDQLQAGFDSAAAGLDEAKALLVAKEREL 177
+ +E+++ R L +S+ Q+ Q + D A A + E
Sbjct: 171 PDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLS 230

Query: 178 AVFNAQLN 185
V ++L+
Sbjct: 231 RVEKSRLD 238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0523TCRTETB1232e-32 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 123 bits (309), Expect = 2e-32
Identities = 89/421 (21%), Positives = 177/421 (42%), Gaps = 19/421 (4%)

Query: 18 SEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAE 77
+ Y + + R + I +F ++L+ + N S+ +I +W++TA+++
Sbjct: 3 TSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTF 62

Query: 78 MIAIPLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWN-LEAMIAFRALQGFFGGALIP 136
I + G LS L ++R LL+ F S++ + + +I R +QG A
Sbjct: 63 SIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPA 122

Query: 137 LAFRLILEFLPENKRAVGMALFGVTATFAPSIGPTLGGWLTEHFSWHYLFYINVPPGLLV 196
L ++ ++P+ R L G +GP +GG + + W YL +P ++
Sbjct: 123 LVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPMITII 180

Query: 197 MAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGNRKDWFGSDLIRNLAII 256
L K+ V + D+ GI+ M++G+ + F + + I+
Sbjct: 181 TVPFLMKLLKKEVRIKG--HFDIKGIILMSVGIVFFML----------FTTSYSISFLIV 228

Query: 257 AAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMALFGAIYLIPLYLSQVHD 316
+ ++ ++FV K DP V+ L F++ + ++ + G + ++P + VH
Sbjct: 229 SVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQ 288

Query: 317 YTPLEIGEVIMWMGFPQLLVL-PLVPRLMQRFDGRYLAAFGFFMFALSYYMNSQMTADYA 375
+ EIG VI++ G +++ + L+ R Y+ G ++S+ S +
Sbjct: 289 LSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFL--LET 346

Query: 376 GPQMIASQVVRALG-QPFILVPIGMLATAHLKPHENPSASTVLNVMRNLGGAFGIALVAT 434
+ +V LG F I + ++ LK E + ++LN L GIA+V
Sbjct: 347 TSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGG 406

Query: 435 L 435
L
Sbjct: 407 L 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0524SACTRNSFRASE392e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.8 bits (90), Expect = 2e-06
Identities = 20/72 (27%), Positives = 29/72 (40%), Gaps = 5/72 (6%)

Query: 81 ASIGRVVVSPAGRGKGLAMPLMQRAIESVLSTWPAAGIQIGAQDYLKS---FYQKLGFSA 137
A I + V+ R KG+ L+ +AIE G+ + QD S FY K F
Sbjct: 90 ALIEDIAVAKDYRKKGVGTALLHKAIEWAKEN-HFCGLMLETQDINISACHFYAKHHFII 148

Query: 138 CS-EMYLEDGIP 148
+ + L P
Sbjct: 149 GAVDTMLYSNFP 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0526HTHFIS357e-121 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 357 bits (918), Expect = e-121
Identities = 134/459 (29%), Positives = 219/459 (47%), Gaps = 41/459 (8%)

Query: 34 LKQAAWNCVKAVSAAEALVLLQKYELRVAIAFISDTNQTALAKEIAIIQAEYPSLHWIAV 93
L +A ++ +AA + + + + + ++ A + I+ P L + +
Sbjct: 23 LSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL-LPRIKKARPDLPVLVM 81

Query: 94 TD-SALEQHCSWLSAANFVDYYHRPFDWNRFADTLGHAWGMAQLTPSKGGKGPAKVLTTI 152
+ + + DY +PFD +G A + PSK + +
Sbjct: 82 SAQNTFMTAIKASEKGAY-DYLPKPFDLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLV 140

Query: 153 KGEHPLLQQLRQRLHKFSQSDETVLLSGETGSGKGLCAKTLHSLSKRHEGPFITVNCGAL 212
G +Q++ + L + Q+D T++++GE+G+GK L A+ LH KR GPF+ +N A+
Sbjct: 141 -GRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAI 199

Query: 213 PTGLIHSTLFGHEKGAFTDADKRYIGHLEQAHGGTLFLDEIADLPLDLQVNLLHFLDDKH 272
P LI S LFGHEKGAFT A R G EQA GGTLFLDEI D+P+D Q LL L
Sbjct: 200 PRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGE 259

Query: 273 IMRIGGNAPIKVDCRLLFASHQDLEAAIDEGRFREDLYHRINVLRLHVPSLRQYSDEVML 332
+GG PI+ D R++ A+++DL+ +I++G FREDLY+R+NV+ L +P LR ++++
Sbjct: 260 YTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPD 319

Query: 333 LAEQFLLE-HSDNCTQFHFSDEARCAMKHYNWPGNVRELRNRIRRAMVLTDDCIITAQLL 391
L F+ + + F EA MK + WPGNVREL N +RR L +IT +++
Sbjct: 320 LVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITREII 379

Query: 392 GLD---SLTANRTSQ---------------------------------DLARCRAEHEAE 415
+ + + + R AE E
Sbjct: 380 ENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYP 439

Query: 416 VLLKAISDHKHNISAAARSLNISRATFYRLLKKCQIKVP 454
++L A++ + N AA L ++R T + +++ + V
Sbjct: 440 LILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVY 478


61Shewana3_0542Shewana3_0547N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0542015-1.940690multi-sensor signal transduction histidine
Shewana3_0543015-2.128866response regulator receiver protein
Shewana3_0544-113-1.783644response regulator receiver modulated
Shewana3_0545-216-0.250241alpha-L-glutamate ligase
Shewana3_0546-117-1.665327hypothetical protein
Shewana3_0547018-2.970711histone family protein DNA-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0542PF06580350.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 34.8 bits (80), Expect = 0.001
Identities = 21/107 (19%), Positives = 37/107 (34%), Gaps = 22/107 (20%)

Query: 610 LVIRNLFSNAIKH---HDQAEGVIKVVCSASNTHYWFSVIDDGPGISTKYHGKVFEMFQT 666
++++ L N IKH G I + + N V + G
Sbjct: 258 MLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGS---------------- 301

Query: 667 LRPRDEVEGSGLGLSLVKKTVESLGGK---IQLESEGRGCCFRFSWP 710
L ++ E +G GL V++ ++ L G I+L + P
Sbjct: 302 LALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0543HTHFIS476e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 46.7 bits (111), Expect = 6e-09
Identities = 23/109 (21%), Positives = 43/109 (39%), Gaps = 10/109 (9%)

Query: 11 TILLVDDDDVDYMAVQRAMKQLRLLNPLVRARDGLEALSILTHSEAIKGSYLILLDLNMP 70
TIL+ DDD + +A+ + + + + + L++ D+ MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGY--DVRITSNAATLWRWIAAGD----GDLVVTDVVMP 58

Query: 71 RMNGFEFLEHIRS-DPTLSSSVVFMLTTSSTDEDRMKAYSHHVAGYMVK 118
N F+ L I+ P L V +++ +T +KA Y+ K
Sbjct: 59 DENAFDLLPRIKKARPDL---PVLVMSAQNTFMTAIKASEKGAYDYLPK 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0544HTHFIS631e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.3 bits (154), Expect = 1e-12
Identities = 36/137 (26%), Positives = 58/137 (42%), Gaps = 6/137 (4%)

Query: 3 LLLIDDDEVDRTAIIRALRQSKLAFNVVEANCAFDGLNLALERHFDGILLDYLLPDANGL 62
+L+ DDD RT + +AL S+ ++V + A D ++ D ++PD N
Sbjct: 6 ILVADDDAAIRTVLNQAL--SRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 63 EVLIKLNSMTQDQTVVVMLSRYEDEKLAQRCIELGAQDFLLK---DEVNSRILSRAIRYA 119
++L ++ D V+VM S A + E GA D+L K I+ RA+
Sbjct: 64 DLLPRIKKARPDLPVLVM-SAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 120 KQRSSMALALRNSHQKL 136
K+R S L
Sbjct: 123 KRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0546OMPADOMAIN310.004 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 31.1 bits (70), Expect = 0.004
Identities = 35/200 (17%), Positives = 65/200 (32%), Gaps = 54/200 (27%)

Query: 47 VAIQGGIDYSHDSGFYAGTWASNVDFGDDTSYELDLYAGYGGNITEDLSYDIGYLYYAYP 106
+ G HD+GF +N + + GY + + +++GY +
Sbjct: 30 TGAKLGWSQYHDTGFI-----NNNGPTHENQLGAGAFGGY--QVNPYVGFEMGYDWLGRM 82

Query: 107 DAEGSID-------------------------FGELHGAITWKWFELSYSQVINAGDDVA 141
+GS++ + L G + W + S V D
Sbjct: 83 PYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGGMV---WRADTKSNVYGKNHDTG 139

Query: 142 AEPLDNKDMSYLAATASFPLTDKLSLSLHYGYSSGDVVESWFGEDNYADYNVTLSADTSM 201
P+ A + +T +++ L Y +++ N D + T+
Sbjct: 140 VSPV-------FAGGVEYAITPEIATRLEYQWTN-----------NIGDAH-TIGTRPDN 180

Query: 202 GTVSFMVSDTDLQGDDAKVV 221
G +S VS QG+ A VV
Sbjct: 181 GMLSLGVSYRFGQGEAAPVV 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0547DNABINDINGHU1093e-35 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 109 bits (274), Expect = 3e-35
Identities = 44/88 (50%), Positives = 66/88 (75%)

Query: 2 NKTELIAKIAENADITKAEAARALKSFEAAITESMKNGDKISIVGFGSFETTTRAARTGR 61
NK +LIAK+AE ++TK ++A A+ + +A++ + G+K+ ++GFG+FE RAAR GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEIQIAEATVPKFKAGKTLRDSV 89
NPQTG+EI+I + VP FKAGK L+D+V
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


62Shewana3_0632Shewana3_0638N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0632-1183.862655methyl-accepting chemotaxis sensory transducer
Shewana3_06330163.879732molydopterin dinucleotide-binding region
Shewana3_06340133.015697polysulfide reductase, NrfD
Shewana3_06351162.9519854Fe-4S ferredoxin
Shewana3_06362162.587604ATPase domain-containing protein
Shewana3_06371192.750847two component LuxR family transcriptional
Shewana3_06382202.494852hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0632BACSURFANTGN290.036 Yersinia/Haemophilus virulence surface antigen sign...
		>BACSURFANTGN#Yersinia/Haemophilus virulence surface antigen

signature.
Length = 322

Score = 28.9 bits (64), Expect = 0.036
Identities = 17/98 (17%), Positives = 39/98 (39%), Gaps = 16/98 (16%)

Query: 248 ASDITQRIIKSHAIRDAANIAHATSQSTVEQANRGVQLLDATVNTSNAIAAQTHKTTESM 307
++ +T+ +I +H ++ ++H+ Q + AT+ + + + + S
Sbjct: 23 SATLTEGVIGAHRVKVETALSHSNLQKKLS----------ATIKHNQSGRSMLDRKLTSD 72

Query: 308 LKLNEQSQSIQAIVATISAIADQTNLLALNAAIEAARA 345
K N++S T S I + L+ + A R
Sbjct: 73 GKANQRSSF------TFSMIMYRMIHFVLSTRVPAVRE 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0636PF06580330.003 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.003
Identities = 32/195 (16%), Positives = 74/195 (37%), Gaps = 33/195 (16%)

Query: 468 LQSVLTLIQQEVSRADSIISRLRNLLKK--RPVSKQLLYLHQLVNDTAPLLAY-ELEQ-- 522
L ++ LI ++ ++A +++ L L++ R + + + L + + +Y +L
Sbjct: 179 LNNIRALILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTV---VDSYLQLASIQ 235

Query: 523 --HHIQLSTNVSGEAYQLPLDEVGMQQLLLNLLKNAADACVQRQQSEVDASKPYQPTIDI 580
+Q ++ + + + +Q L+ N +K+ A P I +
Sbjct: 236 FEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGI------------AQLPQGGKILL 283

Query: 581 DLRYQEHKLLLTVTDNGTGLTEDANLLMQAFYSTKSEGLGLGLVICRDIAESHGGRFSLE 640
+ L V + G+ ++ + +S G GL V R + +G ++
Sbjct: 284 KGTKDNGTVTLEVENTGSLALKN---------TKESTGTGLQNVRER-LQMLYGTEAQIK 333

Query: 641 -TALSGGCQAQVSLP 654
+ G A V +P
Sbjct: 334 LSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0637HTHFIS978e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 97.2 bits (242), Expect = 8e-26
Identities = 29/119 (24%), Positives = 52/119 (43%)

Query: 7 VYLIDDDESVRRSLRFMLESYGLNIRDFDSAEAFFAAIDLSQPGCALVDVRMPGLSGPQL 66
+ + DDD ++R L L G ++R +A + I + DV MP + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 67 HAQLVQHNSPLAVIYLTGHGDVPMAVEALKLGAVDFFQKPADGAKLADAVVKALEHAKA 125
++ + L V+ ++ A++A + GA D+ KP D +L + +AL K
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0638GPOSANCHOR521e-08 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 52.0 bits (124), Expect = 1e-08
Identities = 53/316 (16%), Positives = 102/316 (32%), Gaps = 10/316 (3%)

Query: 598 EYAASEQELRIRLSKAEEALQSAQELQAEAESQLVAINGELDKLSRELTFARTAYKNSRD 657
EL LS A+E L+ + +E S++ + L + L A
Sbjct: 82 ALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSA 141

Query: 658 DLRRLFDEKRSEQDKINKALAERKAFAQQRLTQLDGELKQLKHQHQLWLEEQKEQALEAR 717
++ L EK + KA K + + E ++ LE
Sbjct: 142 KIKTLEAEK---AALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 198

Query: 718 MEKQAYWQEVIGALDNQLGQIKATIDARRESAKAEQKACETWYKNELKSRGVDEDNILKL 777
+E + A L KA + AR+ + + + + E L
Sbjct: 199 LEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 258

Query: 778 KQQIRELETKISRAEQRRSEVLRFDDWY-----QHTWLIRKPKLQTQLSDVKR-AASEID 831
+ + ELE + A + + Q+Q+ + R +
Sbjct: 259 EARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL 318

Query: 832 QQLKAKTQEVKTRRQQLETERKASDAAQIEASENLTKLRAVMRKLAELKLPANNEEAQGS 891
+ ++++ Q+LE + K S+A++ +L R ++L E + E+ + S
Sbjct: 319 DASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL-EAEHQKLEEQNKIS 377

Query: 892 LGERLRQGEDLLLKRD 907
R DL R+
Sbjct: 378 EASRQSLRRDLDASRE 393



Score = 33.5 bits (76), Expect = 0.006
Identities = 50/346 (14%), Positives = 112/346 (32%), Gaps = 27/346 (7%)

Query: 360 WRADMENLSERHKLQTEKHQDIEAAYNARRSKIGEQLNRELEGLHAEQDKQREARDKQRE 419
+ + + K+ D+ A + N EL + ++ DK
Sbjct: 55 VQERADKFEIENNTLKLKNSDLSFNNKAL-----KDHNDELTEELSNAKEKLRKNDKSLS 109

Query: 420 VARADLDALEAQWRSQMDAGKASFSEQEYQFKLNAAELKLRVDGVTYTEEEKLSLAIFDE 479
+ + LEA + D KA + +A L + + ++
Sbjct: 110 EKASKIQELEA---RKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADL----EK 162

Query: 480 RIHRADEEQESCNAKVERLTGEERKLRAKRDQANEALRIASLRVNERQTALDELHHMLFP 539
+ A + +AK++ L E+ L A++ + +AL A + L
Sbjct: 163 ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAA 222

Query: 540 QSHTL--LEFLRKEAQGWEQSLGKVIAPELLHRTDLHPTVTGESDTVFGVHLDLKAIDVP 597
+ LE + A + + I + L +
Sbjct: 223 LAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKAL------------E 270

Query: 598 EYAASEQELRIRLSKAEEALQSAQELQAEAESQLVAINGELDKLSRELTFARTAYKNSRD 657
++ E + + +A+ E Q +N L R+L +R A K
Sbjct: 271 GAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEA 330

Query: 658 DLRRLFDEKRSEQDKINKALAERKAFAQQRLTQLDGELKQLKHQHQ 703
+ ++L ++ + + ++L +++ QL+ E ++L+ Q++
Sbjct: 331 EHQKLEEQNKI-SEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375


63Shewana3_0650Shewana3_0657N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0650-1121.842669two-component response regulator
Shewana3_0651-1122.237972hypothetical protein
Shewana3_0652-1131.320141aspartate kinase III
Shewana3_06530100.334306succinylglutamate desuccinylase/aspartoacylase
Shewana3_0654114-0.350696Mg2 transporter protein, CorA family protein
Shewana3_0655013-0.812990hypothetical protein
Shewana3_0656015-0.721013two component LuxR family transcriptional
Shewana3_0657014-0.656010nitrate/nitrite sensor protein NarQ
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0650HTHFIS832e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 82.6 bits (204), Expect = 2e-20
Identities = 31/131 (23%), Positives = 61/131 (46%), Gaps = 1/131 (0%)

Query: 1 MQNPHILIVEDEAVTRNTLRSIFEAEGYVVTEANDGAEMHKAMQENKINLVVMDINLPGK 60
M IL+ +D+A R L GY V ++ A + + + +LVV D+ +P +
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 NGLLLARELREIN-NIGLIFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLT 119
N L +++ ++ ++ ++ ++ + I E GA DY+ KPF+ EL L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 120 RVNSAGNEVEE 130
+++E+
Sbjct: 121 EPKRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0652CARBMTKINASE310.009 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 30.9 bits (70), Expect = 0.009
Identities = 18/81 (22%), Positives = 27/81 (33%), Gaps = 5/81 (6%)

Query: 202 DYSAALLAEALKASAVEIWTDVAGIYTTDPRLAPNAHPIAEISFNEAAEMATFGAKVLHP 261
D + LAE + A I TDV G + E+ E + G
Sbjct: 216 DLAGEKLAEEVNADIFMILTDVNGAALY--YGTEKEQWLREVKVEELRKYYEEGH--FKA 271

Query: 262 ATILPAVRQQIQVFVGSSKEP 282
++ P V I+ F+ E
Sbjct: 272 GSMGPKVLAAIR-FIEWGGER 291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0656HTHFIS643e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.7 bits (155), Expect = 3e-14
Identities = 27/159 (16%), Positives = 62/159 (38%), Gaps = 9/159 (5%)

Query: 6 SVLVVDDHPLLRKGICQLIASDPDFSLFGEVGGGLDALSAVATDEPDIVLLDLNMKGMTG 65
++LV DD +R + Q S + + +A + D+V+ D+ M
Sbjct: 5 TILVADDDAAIRTVLNQ-ALSRAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 66 LDTLNAMRQEGVTSRIVILTVSDAKQDVIRLLRAGADGYLLKDTEPDLLLDKLKNAMLGH 125
D L +++ +++++ + I+ GA YL K + L+ + A+
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL--- 119

Query: 126 RVISDEVEEYLYELKNAADEQEWISSLTPRELQILQQLA 164
E + +L++ + + + + +I + LA
Sbjct: 120 ----AEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLA 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0657PF06580411e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 40.6 bits (95), Expect = 1e-05
Identities = 26/140 (18%), Positives = 55/140 (39%), Gaps = 17/140 (12%)

Query: 410 INEGVSTAYVQLRELLSTFRLTIKEPNLKN-AMEAMLEQLRANTDI-------KIHLDYK 461
I E + A L L R +++ N + ++ L + + + ++ + +
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQ 245

Query: 462 LSPQWLEAKQHIHILQITREATLNAIKHA-----NASQINIRCYKDDNGMVNISVSDNGI 516
++P ++ + ++Q E N IKH +I ++ KD NG V + V + G
Sbjct: 246 INPAIMDVQVPPMLVQTLVE---NGIKHGIAQLPQGGKILLKGTKD-NGTVTLEVENTGS 301

Query: 517 GIGYLKERDQHFGIGIMHER 536
+ G+ + ER
Sbjct: 302 LALKNTKESTGTGLQNVRER 321


64Shewana3_0861Shewana3_0870N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_0861-1140.428132phospholipase A1
Shewana3_0862-2130.504441uroporphyrinogen-III C-methyltransferase
Shewana3_0863-2130.163149sulfate adenylyltransferase subunit 2
Shewana3_0864-2140.517147sulfate adenylyltransferase subunit 1
Shewana3_0865-2150.113631TrkA domain-containing protein
Shewana3_0866-116-0.594569adenylylsulfate kinase
Shewana3_0867-216-1.041983ATPase domain-containing protein
Shewana3_0868-217-0.753135diguanylate cyclase/phosphodiesterase
Shewana3_0869-214-1.077955hypothetical protein
Shewana3_0870-213-1.943124major facilitator superfamily transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0861PHPHLIPASEA12171e-71 Bacterial phospholipase A1 protein signature.
		>PHPHLIPASEA1#Bacterial phospholipase A1 protein signature.

Length = 289

Score = 217 bits (553), Expect = 1e-71
Identities = 107/300 (35%), Positives = 156/300 (52%), Gaps = 26/300 (8%)

Query: 6 KGIALIGLLTCTGLQAEESLVKGRVQDE-----------LATSERPFVITPHKVNYILPA 54
+G L + + A+E+ VK V D L + PF + P+ NY++
Sbjct: 5 QGWLLPVFMLPMAVYAQEATVK-EVHDAPAVRGSIIANMLQEHDNPFTLYPYDTNYLIYT 63

Query: 55 TYNPDPNMAPFAQDAAENDYTLDEMEAKFQISFKFPLWYNVFGDNGHLFFAYTNQSYWQL 114
+ A + D AEN + E KFQ+S FPLW + G N L +YT +S+WQL
Sbjct: 64 QTSDLNKEAIASYDWAENA---RKDEVKFQLSLAFPLWRGILGPNSVLGASYTQKSWWQL 120

Query: 115 YNKDISSPFRETNHEPEIFMLFNNDWKIGSVTNSFWGFGAVHQSNGKSGLLSRSWNRIYG 174
N + SSPFRETN+EP++F+ F D++ T G H SNG+S SRSWNR+Y
Sbjct: 121 SNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGYNHDSNGRSDPTSRSWNRLYT 180

Query: 175 TMIFDAGPLAFATKVWWRIPEDEKTDIHQPRGDDNPDIDEYIGKAEFVGVYGIDDHRFTL 234
++ + G K W+ + DDNPDI +Y+G + Y + D +
Sbjct: 181 RLMAENGNWLVEVKPWYVVGNT----------DDNPDITKYMGYYQLKIGYHLGDAVLSA 230

Query: 235 TLKTNFKDIDRGSAEFTWSYPIIGNLRLYTQYFNGYGESLIDYNYHNQRIGIGLSLNDIL 294
+ N+ G AE SYPI ++RLYTQ ++GYGESLIDYN++ R+G+G+ LND+
Sbjct: 231 KGQYNWNT-GYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQTRVGVGVMLNDLF 289


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0864TCRTETOQM683e-14 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 68.0 bits (166), Expect = 3e-14
Identities = 47/154 (30%), Positives = 70/154 (45%), Gaps = 17/154 (11%)

Query: 41 VDDGKSTLIGRLLHDSAQIYEDQLASLKNDSAKMGTTGEAIDLALLVDGLQAEREQGITI 100
VD GK+TL LL++S I +L S+ + + D ER++GITI
Sbjct: 12 VDAGKTTLTESLLYNSGAI--TELGSVDKGTTRT-------------DNTLLERQRGITI 56

Query: 101 DVAYRYFSSDKRKFIIADTPGHEQYTRNMATGASTCDLAVILVDARYGVQTQTKRHAFIA 160
F + K I DTPGH + + S D A++L+ A+ GVQ QT+
Sbjct: 57 QTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHAL 116

Query: 161 SLLGIRHFVVAVNKMDLLGFD-EQVFNRIRNDFS 193
+GI + +NK+D G D V+ I+ S
Sbjct: 117 RKMGIPT-IFFINKIDQNGIDLSTVYQDIKEKLS 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0867PF06580531e-09 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 53.3 bits (128), Expect = 1e-09
Identities = 30/146 (20%), Positives = 53/146 (36%), Gaps = 23/146 (15%)

Query: 478 SLVQDMLHTSWLRMKKQFHELNIDISPEIELNSYPGALGQVLENLVSNALTHAFEDV-GN 536
++V L + ++ + + I+P I P L Q L V N + H +
Sbjct: 223 TVVDSYLQLASIQFEDRLQ-FENQINPAIMDVQVPPMLVQTL---VENGIKHGIAQLPQG 278

Query: 537 GQINITAYSLEDAFVAITVTDNGGGMDDATLKQIFDPFFTTRRGKGGTGLGLHLAHQLVT 596
G+I + ++ V + V + G + K TG GL + +
Sbjct: 279 GKILLKGT-KDNGTVTLEVENTGSLA--------------LKNTKESTGTGLQNVRERLQ 323

Query: 597 QLLGG--YIKVTSELGKGTCFTLTIP 620
L G IK++ + GK + IP
Sbjct: 324 MLYGTEAQIKLSEKQGKVNA-MVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0868HTHFIS435e-06 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 42.5 bits (100), Expect = 5e-06
Identities = 23/146 (15%), Positives = 47/146 (32%), Gaps = 18/146 (12%)

Query: 25 AVKILTVDDDINFQRSTAFALSTLTILGSKIELTQAFSYAEACQIVANEDDFAIALVDVV 84
IL DDD + ALS ++ + A + +A D + + DVV
Sbjct: 3 GATILVADDDAAIRTVLNQALSRA-----GYDVRITSNAATLWRWIA-AGDGDLVVTDVV 56

Query: 85 METEDAGLRLVRGIREVLGNEKIRIILLTGQPGMAPVFNVMR----DY-----DINDYWT 135
M E+ L+ I++ + +++++ Q DY D+ +
Sbjct: 57 MPDEN-AFDLLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG 113

Query: 136 KSELSADRLQTILTTNLRSYQQISNI 161
+ + + Q +
Sbjct: 114 IIGRALAEPKRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_0870TCRTETA348e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 34.0 bits (78), Expect = 8e-04
Identities = 31/143 (21%), Positives = 47/143 (32%), Gaps = 12/143 (8%)

Query: 249 VVNLLFAPAIGRFIGRIGERNALTVEYIGLFFVFVSYALVEQPHMAAALY---VIDHLLF 305
++ AP +G R G R L + L V YA++ LY ++ +
Sbjct: 54 LMQFACAPVLGALSDRFGRRPVLL---VSLAGAAVDYAIMATAPFLWVLYIGRIVAGITG 110

Query: 306 AMAIAMKTYFQKIADSKDIAAT---MSVSFTINHIAAVVIPVLLGLLWLSDPALVFYIGA 362
A Y I D + A MS F +A PVL GL+ P F+ A
Sbjct: 111 ATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAG---PVLGGLMGGFSPHAPFFAAA 167

Query: 363 GFAVCSLVLALNVPRHPSPGNET 385
+ + + G
Sbjct: 168 ALNGLNFLTGCFLLPESHKGERR 190


65Shewana3_1019Shewana3_1030N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1019-1130.611271preprotein translocase subunit SecD
Shewana3_10200150.091433preprotein translocase subunit SecF
Shewana3_1021015-0.058982hypothetical protein
Shewana3_10220150.03967123S rRNA methyltransferase J
Shewana3_1023016-0.213505membrane protease FtsH catalytic subunit
Shewana3_1024120-0.618205dihydropteroate synthase
Shewana3_1025432-0.298162phosphoglucosamine mutase
Shewana3_1026636-0.672452triosephosphate isomerase
Shewana3_1027634-0.166175preprotein translocase subunit SecG
Shewana3_10286350.006066**hypothetical protein
Shewana3_1029527-0.053197transcription elongation factor NusA
Shewana3_1030531-0.246473translation initiation factor IF-2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1019SECFTRNLCASE781e-17 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 78.0 bits (192), Expect = 1e-17
Identities = 31/172 (18%), Positives = 82/172 (47%), Gaps = 4/172 (2%)

Query: 442 VTIVEERTIGPTLGAENIQNGFAALGLGMGITLLFMALWYR-RLGWVANVALIANMVILF 500
+ I ++GP + E + +L + + ++ + + + A VAL+ ++++
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 501 GLLALIPGAVLTLPGIAGLVLTVGMAVDTNVLIFERIKDKLKEGRSFALA--IDTGFDSA 558
GL A++ L +A L+ G +++ V++F+R+++ L + ++ L ++ +
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 559 FSTIFDANFTTMITAVVLYSIGNGPIQGFALTLGLGLLTSMFTGIFASRALI 610
S TT++ V + G I+GF + G+ T ++ ++ ++ ++
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIV 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1020SECFTRNLCASE2381e-79 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 238 bits (609), Expect = 1e-79
Identities = 91/305 (29%), Positives = 154/305 (50%), Gaps = 20/305 (6%)

Query: 2 KNINLTKWRYVSSAISIFLMLASLTIIGMKGFNWGLDFTGGVVTEVQLDRRITSSELQPL 61
N + +W++ + +I +M+AS+ + + G N+G+DF GG + I +
Sbjct: 12 TNFDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAA 71

Query: 62 LNAAYQQEVTVISASEP--------------------GRWVLRYADTAQSNVDIAQTLAP 101
L +V + +P G N A
Sbjct: 72 LEPLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAV 131

Query: 102 LGEVQVLNTSIVGPQVGKELAEQGGLALLVAMLCILGYLSYRFEWRLASGALFALVHDVI 161
+++ + VGP+V EL +LL A + I+ Y+ RFEW+ A GA+ ALVHDV+
Sbjct: 132 DPALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVL 191

Query: 162 FVLAFFSLTQMEFNLTVLAAVLAILGYSLNDSIIIADRIRELLIAKPKLAIQEINNQAIV 221
+ F++ Q++F+LT +AA+L I GYS+ND++++ DR+RE LI + ++++ N ++
Sbjct: 192 LTVGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVN 251

Query: 222 ATFSRTMVTSGTTLMTVGALWIMGGGPLEGFSIAMFIGILTGTFSSISVGTSLPEFLGLT 281
T SRT++T TTL+ + + I GG + GF AM G+ TGT+SS+ V ++ F+GL
Sbjct: 252 ETLSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIVLFIGLD 311

Query: 282 PEHYK 286
K
Sbjct: 312 RNKEK 316


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1023HTHFIS340.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 0.002
Identities = 23/82 (28%), Positives = 32/82 (39%), Gaps = 18/82 (21%)

Query: 198 VLMVGPPGTGKTLLAKAIAGESK---VPFFT-----ISGSDFVEMFVGV------GASRV 243
+++ G GTGK L+A+A+ K PF I G GA
Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTR 222

Query: 244 RD-MFEQAKKSAPCIIFIDEID 264
FEQA+ +F+DEI
Sbjct: 223 STGRFEQAEGGT---LFLDEIG 241


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1026adhesinb310.003 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 31.4 bits (71), Expect = 0.003
Identities = 18/95 (18%), Positives = 34/95 (35%), Gaps = 16/95 (16%)

Query: 142 REARRTFEVIAEELDIVIQKNGTMAFDNAIIAY----EPLWAVGTGKSATPEQAQEVHAF 197
+EA+ F I E +++ G F AY +W + T + TP+Q + +
Sbjct: 186 KEAKEKFNNIPGEKKMIVTSEG--CFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTLVEK 243

Query: 198 IRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQ 232
+RK + L+ S ++
Sbjct: 244 LRKT----------KVPSLFVESSVDDRPMKTVSK 268


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1027SECGEXPORT1212e-39 Protein-export SecG membrane protein signature.
		>SECGEXPORT#Protein-export SecG membrane protein signature.

Length = 110

Score = 121 bits (305), Expect = 2e-39
Identities = 64/110 (58%), Positives = 83/110 (75%)

Query: 1 MYEVLVVVYLLVALGLIGLILIQQGKGADMGASFGAGASGTLFGSSGSGNFLTRTTAILA 60
MYE L+VV+L+VA+GL+GLI++QQGKGADMGASFGAGAS TLFGSSGSGNF+TR TA+LA
Sbjct: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60

Query: 61 IAFFTLSLLIGNLSANHAKNEDAWKNLGADEQVTQPVDQATEKSETKIPD 110
FF +SL++GN+++N W+NL A + Q A K + IP+
Sbjct: 61 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN 110


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1030TCRTETOQM694e-14 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 69.1 bits (169), Expect = 4e-14
Identities = 39/133 (29%), Positives = 57/133 (42%), Gaps = 18/133 (13%)

Query: 396 IMGHVDHGKTSLLDYIRRAKVAAGEAG------------------GITQHIGAYHVETEN 437
++ HVD GKT+L + + A E G GIT G + EN
Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67

Query: 438 GMITFLDTPGHAAFTAMRARGAKATDIVVLVVAADDGVMPQTIEAIQHAKAGNVPLIVAV 497
+ +DTPGH F A R D +L+++A DGV QT + +P I +
Sbjct: 68 TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFI 127

Query: 498 NKMDKPEADIDRV 510
NK+D+ D+ V
Sbjct: 128 NKIDQNGIDLSTV 140


66Shewana3_1076Shewana3_1084N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1076-3142.078755TetR family transcriptional regulator
Shewana3_1077-2131.430284RND family efflux transporter MFP subunit
Shewana3_1078-2131.660576hydrophobe/amphiphile efflux-1 (HAE1) family
Shewana3_1079-3151.388404metal dependent phosphohydrolase
Shewana3_1080-3151.061059hypothetical protein
Shewana3_1081-2150.404744AraC family transcriptional regulator
Shewana3_10820100.314812major facilitator superfamily transporter
Shewana3_10830110.419071acriflavin resistance protein
Shewana3_1084113-0.250437RND family efflux transporter MFP subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1076HTHTETR594e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 59.3 bits (143), Expect = 4e-13
Identities = 36/160 (22%), Positives = 60/160 (37%), Gaps = 5/160 (3%)

Query: 23 LAKALEVFWQKGFEGTSLTDLTQAMGINKPSLYAAFGNKEQLFLKAIELYEQRPCAFFYP 82
L AL +F Q+G TSL ++ +A G+ + ++Y F +K LF + EL E
Sbjct: 17 LDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELE 76

Query: 83 SLEK--ETAYQVVEAMLYGAASSLVDESHPQGCLIVQGALACSEAGQAIKDTLITRRRDG 140
K V+ +L S V E + + + C G+ R
Sbjct: 77 YQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHK-CEFVGEMAVVQQAQRNLCL 135

Query: 141 E--QALCQRLQRAKDEGDLPADADPLLLSRYIGTVLQGMA 178
E + Q L+ + LPAD + + + G+
Sbjct: 136 ESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLM 175


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1077RTXTOXIND462e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 45.6 bits (108), Expect = 2e-07
Identities = 23/123 (18%), Positives = 48/123 (39%), Gaps = 5/123 (4%)

Query: 64 SVTLVPRVSGYIASVNFKEGALVKKGDVLFHIDASVFEAEVARLRADLASALSAE---QL 120
S + P + + + KEG V+KGDVL + A EA+ + ++ L A + Q+
Sbjct: 96 SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQI 155

Query: 121 ATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVKAALLR--AELDLDYTQVRAP 178
+ +E + + + E + T+ + + + +L+ + RA
Sbjct: 156 LSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAE 215

Query: 179 IDG 181

Sbjct: 216 RLT 218



Score = 39.8 bits (93), Expect = 2e-05
Identities = 20/102 (19%), Positives = 41/102 (40%), Gaps = 10/102 (9%)

Query: 101 EAEVARLRADLASALSAEQLATNDLERARKLFAQKAVSAELLDTRESNKRQTTAAVASVK 160
E+ ++ L S A + + +LF + + +L RQTT + +
Sbjct: 265 VNELRVYKSQLEQIESEILSAKEEYQLVTQLF-KNEILDKL--------RQTTDNIGLLT 315

Query: 161 AALLRAELDLDYTQVRAPIDGRASYANV-TAGNYVSAGQSVL 201
L + E + +RAP+ + V T G V+ ++++
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLM 357


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1078ACRIFLAVINRP10350.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1035 bits (2678), Expect = 0.0
Identities = 419/1043 (40%), Positives = 640/1043 (61%), Gaps = 18/1043 (1%)

Query: 2 LSQFFIKRPIFAAVLSLLFFITGAIAVWQLPITEYPEVVPPTVVVTANYPGANPKVIAET 61
++ FFI+RPIFA VL+++ + GA+A+ QLP+ +YP + PP V V+ANYPGA+ + + +T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 62 VASPLEQEINGVEDMLYMSSQATSDGRMTLTITFAIGTDVDRAQTQVQSRVDRAMPRLPQ 121
V +EQ +NG+++++YMSS + S G +T+T+TF GTD D AQ QVQ+++ A P LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 EVQRLGIVTEKSSPDLTMVVHLLSPDNRYDMLYLSNYAALNVKDELARIKGVGAVRLFGA 181
EVQ+ GI EKSS MV +S + +S+Y A NVKD L+R+ GVG V+LFG
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 182 GEYSLRIWLDPNKVSALGMSPAEIIAAVREQNQQAAAGSLGAQPSGNA-DFQLLINVKGR 240
+Y++RIWLD + ++ ++P ++I ++ QN Q AAG LG P+ I + R
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 241 LTELSEFEDIIIKVGQNGEVIRLKDVARVELGATSYALRSLLDNKDAVAIPVFQASGSNA 300
EF + ++V +G V+RLKDVARVELG +Y + + ++ K A + + A+G+NA
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 301 IQISDDVRAEMARLAKSFPEGLQYEIVYDPTVFVRGSIHAVVKTLLEAVLLVVLVVVLFL 360
+ + ++A++A L FP+G++ YD T FV+ SIH VVKTL EA++LV LV+ LFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 361 QTWRASIIPLVAVPVSLVGTFAFMHLMGFSLNALSLFGLVLAIGIVVDDAIVVVENVERN 420
Q RA++IP +AVPV L+GTFA + G+S+N L++FG+VLAIG++VDDAIVVVENVER
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 421 IAS-GLSPIAATQKAMKEVTGPIVATTLVLAAVFIPTAFMSGLTGQFYKQFALTITISTF 479
+ L P AT+K+M ++ G +V +VL+AVFIP AF G TG Y+QF++TI +
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 480 ISAINSLTLSPALSALLLKGHDAPKDALTRLMDKLFGGWLFTPFNRLFNRASEGYGYLVR 539
+S + +L L+PAL A LLK A G F FN F+ + Y V
Sbjct: 480 LSVLVALILTPALCATLLKPVSAE--------HHENKGGFFGWFNTTFDHSVNHYTNSVG 531

Query: 540 KVIRFGGIIGLVYLGMVALTGVQFVNTPTGYVPGQDKQYLVAFAQLPDAASLERTDAVIK 599
K++ G L+Y +VA V F+ P+ ++P +D+ + QLP A+ ERT V+
Sbjct: 532 KILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLD 591

Query: 600 KMSDIALNH--PGVAHSIAFPGLSINGFTNSPNSGVVFVALDDFELRKSPELSANAIAGQ 657
+++D L + V G S +G + N+G+ FV+L +E R E SA A+ +
Sbjct: 592 QVTDYYLKNEKANVESVFTVNGFSFSG--QAQNAGMAFVSLKPWEERNGDENSAEAVIHR 649

Query: 658 LNQQFAGIQDAFIAIFPPPPVQGLGTIGGFRLQIQDRANLGYEALYQVTQQVMYKAWADP 717
+ I+D F+ F P + LGT GF ++ D+A LG++AL Q Q++ A P
Sbjct: 650 AKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHP 709

Query: 718 -QLAGIFSSYQVNVPQLELDIDRTKAKQQAVSLDQIFQTLQTYMGSTYVNDFNRFGRTYQ 776
L + + + Q +L++D+ KA+ VSL I QT+ T +G TYVNDF GR +
Sbjct: 710 ASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKK 769

Query: 777 VNMQADEAFRQSPQQISQLKVPNVNGDMIPLGSFINVSQSAGPDRVMHYNGFTTAEINGG 836
+ +QAD FR P+ + +L V + NG+M+P +F G R+ YNG + EI G
Sbjct: 770 LYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGE 829

Query: 837 PAPGVSSGQAQAAIEKILAETLPIGMTYEWTELTYQQILAGNTGLLVFPLVILLVFMVLA 896
APG SSG A A +E + ++ LP G+ Y+WT ++YQ+ L+GN + + ++VF+ LA
Sbjct: 830 AAPGTSSGDAMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLA 888

Query: 897 AQYESLSLPLAIILIIPMTLLSALSGVLIYGGDNNIFTQIGLIVLVGLATKNAILIVEFA 956
A YES S+P++++L++P+ ++ L ++ N+++ +GL+ +GL+ KNAILIVEFA
Sbjct: 889 ALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFA 948

Query: 957 KEKQDH-GMEVMESILEAARLRLRPILMTSIAFIMGVVPMVFSTGAGAEMRQAMGVAVFA 1015
K+ + G V+E+ L A R+RLRPILMTS+AFI+GV+P+ S GAG+ + A+G+ V
Sbjct: 949 KDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008

Query: 1016 GMIGVTLFGLILTPLFYYALAKR 1038
GM+ TL + P+F+ + +
Sbjct: 1009 GMVSATLLAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1082TCRTETB642e-13 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 64.1 bits (156), Expect = 2e-13
Identities = 51/237 (21%), Positives = 105/237 (44%), Gaps = 15/237 (6%)

Query: 7 LWLCVLLMMFPQIMETIYSPALPNIAENFAVSVTSASQTLSVYFIAFAVGVFCWGRLADI 66
+WLC+L + E + + +LP+IA +F S + + + + F++G +G+L+D
Sbjct: 17 IWLCILSFFSV-LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 67 IGRRNAMLAGLLCYAIGSACALM-ISDFTLLLFARVLSAFGAA----VGSVITQTMMRDS 121
+G + +L G++ GS + S F+LL+ AR + GAA + V+ +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 122 YNGEELAKVFSVMGMSLGISPIIGLLLGSILSAYWGYQGVFVALMVSAIVLLFLSVKSLP 181
G+ + S++ M G+ P IG ++ + +W Y + + + I+ + +K L
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAHYI--HWSY---LLLIPMITIITVPFLMKLLK 190

Query: 182 ETKPEHTQKIAIVELAIKMLTDIGIIKNTLLVASFNLMWFSYFSLAPFMFEARGLST 238
+ I + L +GI+ L S+++ + L+ +F
Sbjct: 191 KEV-RIKGHFDIKGII---LMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKV 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1083ACRIFLAVINRP7790.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 779 bits (2012), Expect = 0.0
Identities = 308/1032 (29%), Positives = 517/1032 (50%), Gaps = 28/1032 (2%)

Query: 3 LSDVSVKRPVVAIVLSLLLCVFGLVSFTKLSVREMPDVESPVVTVSTSYSGASAAIMESQ 62
+++ ++RP+ A VL+++L + G ++ +L V + P + P V+VS +Y GA A ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 ITKTLEDELTGISGIDEITSTT-RNGSSRITVKFLLGWNLTEGVSDVRDAVARAQRRLPE 121
+T+ +E + GI + ++ST+ GS IT+ F G + V++ + A LP+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 DANDPVVSKDNGSGEPSVYVNLSSSVMDRTQ--LTDYAQRVLEDRFSLISGVSSISISGG 179
+ +S + S + S TQ ++DY ++D S ++GV + + G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 180 LYKVMYVKLRPEQMAGRNVTVTDIINALRKENVETPGGQVRNDTTV------MSVRTKRL 233
Y + + L + + +T D+IN L+ +N + GQ+ + S+ +
Sbjct: 181 QYAMR-IWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 234 YYTPKDFDYLVVRTASDGTPIYLKDVADVAVGAQNENSTFKSDGIVNLSLGVITQSDANP 293
+ P++F + +R SDG+ + LKDVA V +G +N N + +G LG+ + AN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 LIVAQEVHKEVDRIQDFLPEGTSLVVDFDSTVFIDRSINEVYNTLYVTGALVVLVLYIFI 353
L A+ + ++ +Q F P+G ++ +D+T F+ SI+EV TL+ LV LV+Y+F+
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 GQARATLIPAVTVPVSLISAFIAANMFGYSINLLTLMALILAIGLVVDDAIVVVENIFHH 413
RATLIP + VPV L+ F FGYSIN LT+ ++LAIGL+VDDAIVVVEN+
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 I-ERGEEPLLAAYKGTREVGFAVVATTAVLVMVFLPISFMEGMVGLLFTEFSVMLAVSVL 472
+ E P A K ++ A+V VL VF+P++F G G ++ +FS+ + ++
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 FSSLIALTLTPVLSSKLLKANVK-----PNRFNRFVDSGFARMEKVYRVGVTQAIRFKWL 527
S L+AL LTP L + LLK F + ++ F Y V + +
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 528 APLVILACVGGSAWLMQQVPSQLAPQEDRGVLFAFVKGAEGTSYNRMTANMDIVEDRLMP 587
L+ V G L ++PS P+ED+GV ++ G + R +D V D +
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 588 LLGQGVLRSFSVQAPAFGGRAGDQTGFVIMQLEDWEHRHVTAQQALGIIS---NALKDIP 644
V F+V +F G+ G + L+ WE R+ A +I L I
Sbjct: 600 NEKANVESVFTVNGFSFSGQ-AQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 645 DVMVRPM-MPGFRGQ-SSEPVQFVL---GGSDYAELFKWAQVLKEEANASP-MMEGADLD 698
D V P MP ++ F L G + L + L A P + +
Sbjct: 659 DGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 699 YAETTPELIVTVDKERAAELGISVDEVSQTLEVMLGGRKETTYVDRGEEYDVYLRGDENS 758
E T + + VD+E+A LG+S+ +++QT+ LGG ++DRG +Y++ D
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 759 FNNVGDLSQIYMRSAKGELVTLDTLTHIEEVASAQKLSHTNKQKSITLKANISKGYTLGE 818
D+ ++Y+RSA GE+V T V + +L N S+ ++ + G + G+
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 819 ALKFLDNKAIELLPKDISIGYTGESKDFKENQSSILIVFGLALLVAYLVLAAQFESFINP 878
A+ ++N A + LP I +TG S + + + + ++ +V +L LAA +ES+ P
Sbjct: 839 AMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIP 897

Query: 879 LVVMFTVPMGVFGGFLGLLVTSQGINIYSQIGMIMLIGMVTKNGILIVEFANQLRDR-GL 937
+ VM VP+G+ G L + +Q ++Y +G++ IG+ KN ILIVEFA L ++ G
Sbjct: 898 VSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGK 957

Query: 938 ALDKAIIDASTRRLRPILMTAFTTLVGAVPLIFSSGAGSESRIAVGTVVFFGMAFATFVT 997
+ +A + A RLRPILMT+ ++G +PL S+GAGS ++ AVG V GM AT +
Sbjct: 958 GVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLA 1017

Query: 998 LFVIPAMYRLIS 1009
+F +P + +I
Sbjct: 1018 IFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1084RTXTOXIND515e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 50.6 bits (121), Expect = 5e-09
Identities = 21/108 (19%), Positives = 46/108 (42%), Gaps = 3/108 (2%)

Query: 50 PLAQSISLIGKLA-ADRAVVIAPQVTGKIKQIAVTSNQAVKKGQLLIELDDMKAQAAVAE 108
+ + GKL + R+ I P +K+I V ++V+KG +L++L + A+A +
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 109 ANAFLNDETRKLREFEKLISRNAITQTEIDAQKASVDIARARLASAQA 156
+ L +L + I +I ++ K + ++ +
Sbjct: 139 TQSSL--LQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEV 184


67Shewana3_1101Shewana3_1109N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1101-212-0.872405thiamine-monophosphate kinase
Shewana3_1102-215-1.715583phosphatidylglycerophosphatase
Shewana3_1103-215-2.169825hypothetical protein
Shewana3_1104-314-0.772804recombination and repair protein
Shewana3_1105-215-0.665332transporter
Shewana3_1106015-0.148758LysR family transcriptional regulator
Shewana3_1107-3120.413819hypothetical protein
Shewana3_1108-3110.371876hypothetical protein
Shewana3_1109-3110.548545hybrid sensory histidine kinase BarA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1101TYPE3IMQPROT290.006 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 29.0 bits (65), Expect = 0.006
Identities = 10/39 (25%), Positives = 17/39 (43%)

Query: 71 LSDLAAMGAEPAWMTLALTLPEVNEAWLSGFSEGLFEAA 109
+ DL G + ++ L L+ A + G GLF+
Sbjct: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTV 39


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1103ACRIFLAVINRP290.020 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.4 bits (66), Expect = 0.020
Identities = 24/131 (18%), Positives = 50/131 (38%), Gaps = 13/131 (9%)

Query: 127 RLPNIPVMFVDLEDWHE-NGHVLTALELDNENTSHLDFNETLIEESKRIATLLSNDLHLI 185
+ N + FV L+ W E NG +A + + L I + I + + L
Sbjct: 619 QAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGK----IRDGFVIPFNMPAIVELG 674

Query: 186 NSYLPDPFYMSFNQQPQEQLCERDKYKARLTTVARQH--NLNTRNLHIEEGLPEDTI--- 240
+ D + + L + + +L +A QH +L + + E + +
Sbjct: 675 TATGFDFELIDQAGLGHDALTQ---ARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVD 731

Query: 241 AKEARRLNVNM 251
++A+ L V++
Sbjct: 732 QEKAQALGVSL 742


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1104GPOSANCHOR371e-04 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 37.4 bits (86), Expect = 1e-04
Identities = 44/247 (17%), Positives = 79/247 (31%), Gaps = 19/247 (7%)

Query: 138 KSEHQLTLLDSYANHRLLIDTVAASYQRCKQIEAELKQLEASQQERIARKQLVQYQVEEL 197
SE + + A L + + A++K LEA + ARK ++ +E
Sbjct: 108 LSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA 167

Query: 198 DEFDLKVGEFEEIEQEHKRLANGTELVDSCQASLFLLTDGEESNIESLLNKAVGLAENLQ 257
+ K L +++ QA L + + + L+
Sbjct: 168 MN------FSTADSAKIKTLEAEKAALEARQAEL----EKALEGAMNFSTADSAKIKTLE 217

Query: 258 SYDPALTNVSTMLNEALIQVQESAGELQHYLSKLELDPAHFAYLEERLSKAMQLARKHHV 317
+ AL L +AL + + LE A A LE R ++ +
Sbjct: 218 AEKAALAARKADLEKALEGAMNFSTADSAKIKTLE---AEKAALEARQAELEKALEGAMN 274

Query: 318 SPDKLAEHHLALKSELTTLDDDENKLEDIQRQVEASKVAYLANAQKLSQSRARYAK---E 374
+ L++E L+ ++ LE Q + + + L SR + E
Sbjct: 275 FSTADSAKIKTLEAEKAALEAEKADLE---HQSQVLNANRQSLRRDLDASREAKKQLEAE 331

Query: 375 LDKLVTQ 381
KL Q
Sbjct: 332 HQKLEEQ 338



Score = 32.0 bits (72), Expect = 0.007
Identities = 33/203 (16%), Positives = 72/203 (35%), Gaps = 3/203 (1%)

Query: 161 ASYQRCKQIEAELKQLEASQQE-RIARKQLVQYQVEELDEFDLKVGEFEEIEQEHKRLAN 219
A + K +EAE L A + + A + + + + + E +E L
Sbjct: 208 ADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEK 267

Query: 220 GTELVDSCQASLFLLTDGEESNIESLLNKAVGLAENLQSYDPALTNVSTMLNEALIQVQE 279
E + + E+ +L + L Q + ++ L+ + ++
Sbjct: 268 ALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQ 327

Query: 280 SAGELQHYLSKLELDPAHFAYLEERLSKAMQLARKHHVSPDKLAEHHLALKSELTTLDDD 339
E Q + ++ A L L + + ++ KL E + ++ +L D
Sbjct: 328 LEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRD 387

Query: 340 ENKLEDIQRQVEASKVAYLANAQ 362
+ + ++QVE K AN++
Sbjct: 388 LDASREAKKQVE--KALEEANSK 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1108FLAGELLIN280.020 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 28.5 bits (63), Expect = 0.020
Identities = 14/48 (29%), Positives = 22/48 (45%), Gaps = 1/48 (2%)

Query: 94 AMDAVVALSTLLGAIQTDLEEDITNISKLSSSTVANYIETISDVDLTD 141
A+ V A+ + LGAIQ + ITN+ ++ + I D D
Sbjct: 427 ALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSAR-SRIEDADYAT 473


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1109HTHFIS657e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.9 bits (158), Expect = 7e-13
Identities = 27/124 (21%), Positives = 48/124 (38%), Gaps = 2/124 (1%)

Query: 680 QSLTVLAVDDNFANLKLIDTLLNELVTTVIAVNSGEEAVKQAKSRTFDLIFMDIQMPGTD 739
T+L DD+ A +++ L+ V ++ + + DL+ D+ MP +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 740 GISATKQIRQGSMNRNTPIIAVTAHAIAEERELILGSGMDGYLPKPIDEAALKAEINRWI 799
+I+ + P++ ++A G YLPKP D L I R +
Sbjct: 62 AFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 800 TRPK 803
PK
Sbjct: 120 AEPK 123


68Shewana3_1149Shewana3_1160N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_11491190.781441hypothetical protein
Shewana3_11501150.590305methyl-accepting chemotaxis sensory transducer
Shewana3_1151-2120.342319sigma 54 modulation protein/30S ribosomal
Shewana3_1152-2140.613050two component LuxR family transcriptional
Shewana3_1153-1140.482789putative signal transduction histidine kinase
Shewana3_11540120.209738isochorismatase hydrolase
Shewana3_1155-2100.129323hypothetical protein
Shewana3_1156-213-0.076200methyl-accepting chemotaxis sensory transducer
Shewana3_1157-213-0.153388hypothetical protein
Shewana3_1158-213-0.171520TetR family transcriptional regulator
Shewana3_1159-213-0.387665NADH:flavin oxidoreductase
Shewana3_1160014-0.315172ATP-dependent protease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1149YERSINIAYOPE260.042 Yersinia virulence determinant YopE protein signature.
		>YERSINIAYOPE#Yersinia virulence determinant YopE protein signature.

Length = 219

Score = 25.8 bits (56), Expect = 0.042
Identities = 12/70 (17%), Positives = 31/70 (44%), Gaps = 4/70 (5%)

Query: 17 SAVTSYFHAVIRWIKQ---DQTQFGLQVFVRATAALLAGYIAAATLACMLTQVLPMSRFE 73
++S H+VI +I++ + + + A + + + ++ + + LP +
Sbjct: 61 ERLSSVAHSVIGFIQRMFSEGSHKPVVTPAPTPAQMPSPTSFSDSIKQLAAETLPKYMQQ 120

Query: 74 -STLTANMLA 82
++L A ML
Sbjct: 121 LNSLDAEMLQ 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1152HTHFIS763e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.0 bits (187), Expect = 3e-18
Identities = 19/116 (16%), Positives = 52/116 (44%), Gaps = 2/116 (1%)

Query: 18 IRVGLVEDQQLVRQGIASLIAISQHIEVSWQAENGQEALKRLQTDAVDVLLSDIRMPVLD 77
+ + +D +R + ++ + + N + + D++++D+ MP +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRI--TSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 78 GISLLKQLRAAQNSIPVIMLTTFDDSELFLNSLQAGANGFLLKDVSLDKLLEAIET 133
LL +++ A+ +PV++++ + + + + GA +L K L +L+ I
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1153PF06580446e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 44.1 bits (104), Expect = 6e-07
Identities = 20/97 (20%), Positives = 44/97 (45%), Gaps = 14/97 (14%)

Query: 293 LVLQEGISNAVRHG-----HANQLTLSMQEEQAELIICLKDNGQGI--SQSPSQGVGLSS 345
+++Q + N ++HG ++ L ++ + + +++ G + S G GL +
Sbjct: 258 MLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQN 317

Query: 346 MQERLSPFHGSARLQANHAGVDSSRTQGC-SLMIRLP 381
++ERL +G A + S QG + M+ +P
Sbjct: 318 VRERLQMLYG------TEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1154ISCHRISMTASE357e-05 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 35.4 bits (81), Expect = 7e-05
Identities = 33/125 (26%), Positives = 50/125 (40%), Gaps = 18/125 (14%)

Query: 8 KTALLIIDMQQ---GLFYADAPPFNREQVLNNINLLIANAREAGAPIWAVRHTG---PE- 60
+ LLI DMQ F A A P ++ NI L + G P+ G P+
Sbjct: 30 RAVLLIHDMQNYFVDAFTAGASPV--TELSANIRKLKNQCVQLGIPVVYTAQPGSQNPDD 87

Query: 61 --------GSPIAAGTANWQLIESLAINPQLDNIFDKTKPSCFYQTGFAEALTHEGISEL 112
G + +G ++I LA D + K + S F +T E + EG +L
Sbjct: 88 RALLTDFWGPGLNSGPYEEKIITELAPEDD-DLVLTKWRYSAFKRTNLLEMMRKEGRDQL 146

Query: 113 VIVGM 117
+I G+
Sbjct: 147 IITGI 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1158HTHTETR551e-11 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 54.6 bits (131), Expect = 1e-11
Identities = 32/167 (19%), Positives = 65/167 (38%), Gaps = 3/167 (1%)

Query: 2 RNAEFDRAQVLRGAMAAFMHKGYTKTSMQDLTQATGLHPGSIYCAFSNKRGLLIAAIEQY 61
+ A+ R +L A+ F +G + TS+ ++ +A G+ G+IY F +K L E
Sbjct: 7 QEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELS 66

Query: 62 QQDRDEQFKLIFSN-GRPVLGNLKTYLDNIVVECLSCDSQQACLLTKALNEIAEQDDEIQ 120
+ + E + L L+ L +++ ++ + ++ + + +
Sbjct: 67 ESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVV 126

Query: 121 NIISQNLMLWQYAL-TAQFELADSQGMLHGELNSEQRAQYLMMGIYG 166
+NL L Y + ML +L + RA +M G
Sbjct: 127 QQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTR-RAAIIMRGYIS 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1160HTHFIS320.012 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.7 bits (72), Expect = 0.012
Identities = 13/39 (33%), Positives = 17/39 (43%), Gaps = 6/39 (15%)

Query: 339 LFGYVENATFRGTVFTDFSLIRPGSLHKANGGVLLMDAI 377
LFG+ + A FT G +A GG L +D I
Sbjct: 208 LFGHEKGA------FTGAQTRSTGRFEQAEGGTLFLDEI 240


69Shewana3_1288Shewana3_1302N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_128834412.700136conjugal transfer protein TrbL
Shewana3_128923710.376863conjugal transfer protein TrbF
Shewana3_12900267.097679conjugal transfer protein TrbG/VirB9/CagX
Shewana3_1291-1215.299307conjugation TrbI family protein
Shewana3_1292-2182.554946hypothetical protein
Shewana3_1293-3171.960331methyl-accepting chemotaxis sensory transducer
Shewana3_1294-2182.081469hypothetical protein
Shewana3_1295-2172.099199acriflavin resistance protein
Shewana3_1296-220-4.721005RND family efflux transporter MFP subunit
Shewana3_1297-124-6.581283TetR family transcriptional regulator
Shewana3_1298-122-5.838994hypothetical protein
Shewana3_1299122-6.292929hypothetical protein
Shewana3_1300125-6.449654hypothetical protein
Shewana3_1301226-6.634445hypothetical protein
Shewana3_1302323-4.119755polysaccharide biosynthesis protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1288PRTACTNFAMLY300.020 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 30.4 bits (68), Expect = 0.020
Identities = 35/154 (22%), Positives = 53/154 (34%), Gaps = 23/154 (14%)

Query: 254 GIFGPGIATGLVSGAPQL----GAGAMAGAAVGAVGTGVAIGAAATGVGAAVAAGARMAP 309
G FGPG ++ G + + +A + V A G AI G V+ G+ AP
Sbjct: 281 GGFGPGGFGPVLDGWYGVDVSGSSVELAQSIVEAPELGAAI-RVGRGARVTVSGGSLSAP 339

Query: 310 AAAKLAGAGARAATSAAGNARSAFQAGSTAAGGG-------------AKGAAAGLGNVAK 356
+ GAR A QAG+ A G G A G++
Sbjct: 340 HGNVIETGGARRFAPQAAPLSITLQAGAHAQGKALLYRVLPEPVKLTLTGGADAQGDIVA 399

Query: 357 TSAQAASRRVASGASAAGQKMTSSFRAGWNGSSD 390
T + G S + + +A W G++
Sbjct: 400 TELPS-----IPGTSIGPLDVALASQARWTGATR 428


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1290PF03544280.043 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 28.0 bits (62), Expect = 0.043
Identities = 13/73 (17%), Positives = 21/73 (28%)

Query: 26 PPPTISLDESVLAQPLPEPLAPVEVVAVPEPLALPAQLKPLPEVDAAPAAPEPADEKVRV 85
PP + + +P PEP E + + KP P+ +P + V
Sbjct: 62 PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPV 121

Query: 86 SRANAEARIAPTR 98
A
Sbjct: 122 ESRPASPFENTAP 134


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1293IGASERPTASE340.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.3 bits (78), Expect = 0.002
Identities = 35/264 (13%), Positives = 85/264 (32%), Gaps = 35/264 (13%)

Query: 393 QASVQSIEQQASKAQRIAKQNGEEAQALMQQTDQIATAIEEMSTSIRDVANHAQDGANQS 452
+V+ EQ A++ ++ +EA++ ++ Q AQ G+
Sbjct: 1048 SKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEV--------------AQSGSETK 1093

Query: 453 QQVDLAAKEGQQQQTQVVQDLLKLSQQLSSSHQAVEKVSQE-SEAISKVTEVINSIAEQT 511
+ KE + + + Q + QE SE + E +
Sbjct: 1094 ETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAE-----PARE 1148

Query: 512 NLLALNAAIEAARAGEQGRGFAVVADEVRTLAQRTQSSI---LEISQTIDKLQSQVKTTT 568
N +N ++ + A+ T S++ + S T++ S V+
Sbjct: 1149 NDPTVNIKEPQSQTNTTA--------DTEQPAKETSSNVEQPVTESTTVNTGNSVVENPE 1200

Query: 569 SQMAQSHQLGIASANQGEETGKQLEEITRRIGELAISSRNIASATEQQSSVAQEITHNLH 628
+ + Q + S + + + + + +++ +S+VA + +
Sbjct: 1201 NTTPATTQPTVNSESSNKPKNRHRRSVRSV----PHNVEPATTSSNDRSTVALCDLTSTN 1256

Query: 629 QISELANEGEHRAAETVNSANDLS 652
+ L++ +N +S
Sbjct: 1257 TNAVLSDARAKAQFVALNVGKAVS 1280


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1295ACRIFLAVINRP380e-117 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 380 bits (977), Expect = e-117
Identities = 208/1046 (19%), Positives = 431/1046 (41%), Gaps = 52/1046 (4%)

Query: 1 MIKAFVENGRLVSLVIALLLVAGFGAMSSLPRTEDPHITNRFASVITPYPGASAERVEAL 60
M F+ ++ +L++AG A+ LP + P I SV YPGA A+ V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTEVLENQLRRLEEIKLIQSTS-RPGISVIQLELKDTVTDTDPVWSR--ARDLLADARNT 117
VT+V+E + ++ + + STS G I L + TDP ++ ++ L A
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQS---GTDPDIAQVQVQNKLQLATPL 117

Query: 118 LPDGIQTPTL-DDQVGYAYTAILSLVWNDSSQPRVDMLNRYAKELQSRLRLLSGTDFVKL 176
LP +Q + ++ +Y + V ++ + D+ + A ++ L L+G V+L
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 177 YGAPEEEILVQLDGYKMSQLQLTPGTIAKILSSADSKIAAGEINN------NHFRALVEV 230
+GA + + + LD +++ +LTP + L + +IAAG++ A +
Sbjct: 178 FGA-QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 231 SGELDSQSRIRQVPLKVDAQGQIIRLGDIAHISRQPKTPADSIALVDGEQGVFVAARMLN 290
+ +V L+V++ G ++RL D+A + + + IA ++G+ + ++
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENY-NVIARINGKPAAGLGIKLAT 295

Query: 291 NTRVDIWQGQVKQLVDEFNQELPANIKVQWLFEQNSYTSERLGGLIVNLLQGFVIILAVL 350
+K + E P +KV + ++ + + ++ L + +++ V+
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVM 355

Query: 351 LLTLG-LRNAIIVALSLPLTALFTLACMKYIGLPIHQMSVTGLVVALGIMVDNAIVIVDA 409
L L +R +I +++P+ L T A + G I+ +++ G+V+A+G++VD+AIV+V+
Sbjct: 356 YLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVEN 415

Query: 410 IAQRRQ-QGMSRLSAVSETLHHLWLPLAGSTITTILAFAPIVLMPGAAGEFVGGIAMSVM 468
+ + + A +++ + L G + F P+ G+ G +++++
Sbjct: 416 VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIV 475

Query: 469 FALLGSYVISHTLIAGLAGRF--SLEGKHP-------VWYQHGINVPLVSGYFQASLRFA 519
A+ S +++ L L + +H W+ + V+ Y S+
Sbjct: 476 SAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFD-HSVNHY-TNSVGKI 533

Query: 520 LNRPLLSATFIGIIPLLGFYASGKMTEQFFPPSDRDMFQIELYLAPHVSLENTLNQV-QL 578
L +I ++ F P D+ +F + L + E T + Q+
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 579 MDKQLHQIEGIIQVDWVVGGNTPSFYYNLTQRQQGATNYAQAMVK-----ASDFERANAL 633
D L + ++ + V G ++ + + Q A A +K D A A+
Sbjct: 594 TDYYLKNEKANVESVFTVNG------FSFSGQAQNA-GMAFVSLKPWEERNGDENSAEAV 646

Query: 634 IPELQQTLDK---AFPEAQVLVRKLEQGPPFNAPVELM-IFGPNLETLRSLGDEVRNILA 689
I + L K F + +E G EL+ G + L +++ + A
Sbjct: 647 IHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAA 706

Query: 690 ATP-DVLHTRATLSAGAPKVWLQVNEDASLISGLTLTDIARQVQMATTGVIGGSVLEQTE 748
P ++ R + L+V+++ + G++L+DI + + A G +++
Sbjct: 707 QHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGR 766

Query: 749 SLPIRVRLGDTSREQASRLSEIQLVTPSGTAVPLSALSHNEVQVSRGAIPRRNGQRVNTI 808
+ V+ R + ++ + + +G VP SA + + + R NG I
Sbjct: 767 VKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEI 826

Query: 809 EAYIVSGVLPAQVLNDVKAKVAAISLPAGYRIEIGGESAKRNEAVGNLLSNLILVVTLLL 868
+ G + ++ A LPAG + G S + + + + + ++
Sbjct: 827 QGEAAPGTSSGDAMALMEN--LASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVF 884

Query: 869 ATVVLSFNSFRLTAIILLSALQSAGLGLLAVYVFGYPFGFPVIIALLGLMGLAINAAIVI 928
+ + S+ + ++L LLA +F ++ LL +GL+ AI+I
Sbjct: 885 LCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILI 944

Query: 929 LAELEDTDNARA-GDKEVIITTVSSCGRHISSTTITTVGGFIPLII---AGGGFWPPFAI 984
+ +D G E + V R I T++ + G +PL I AG G I
Sbjct: 945 VEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGI 1004

Query: 985 AIAGGTLLTTLLSLVWVPTMYLLLMK 1010
+ GG + TLL++ +VP ++++ +
Sbjct: 1005 GVMGGMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1296RTXTOXIND415e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.4 bits (97), Expect = 5e-06
Identities = 30/117 (25%), Positives = 51/117 (43%), Gaps = 4/117 (3%)

Query: 75 SGKLSELTVDSGAKVTQGQVLAKLDTRLLDAEHQEIQASLAQTQADVDLATSTLNRNLEL 134
+ + E+ V G V +G VL KL +A+ + Q+SL Q + + L+R++EL
Sbjct: 104 NSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ-TRYQILSRSIEL 162

Query: 135 KKSGYVSEQLLDENRTQLASLE-AAKKRLLASQRANQLKRDKSQLLAPFDGIISQRQ 190
K + L DE Q S E + L ++ + + K Q D ++R
Sbjct: 163 NKLPELK--LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217



Score = 37.1 bits (86), Expect = 9e-05
Identities = 27/145 (18%), Positives = 51/145 (35%), Gaps = 16/145 (11%)

Query: 101 RLLDAEHQ--EIQASLAQTQADVDLATSTLNR---NLELKKSGYVSEQL--LDENRTQLA 153
+L+ E++ E L ++ ++ S + +L + +E L L + +
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 154 SLEAAKKRLLASQRANQLKRDKSQLLAPFDGIISQRQ-HNLGEVVAAGSPVFTLVGSVNT 212
L N+ ++ S + AP + Q + H G VV + +V +T
Sbjct: 313 LLTLEL-------AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDT 365

Query: 213 -EAYIGVPVAVAQQFVNGQNVTVSV 236
E V GQN + V
Sbjct: 366 LEVTALVQNKDIGFINVGQNAIIKV 390


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1297HTHTETR733e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 73.1 bits (179), Expect = 3e-18
Identities = 40/197 (20%), Positives = 68/197 (34%), Gaps = 5/197 (2%)

Query: 11 RSEQKKQQVLVAAIDLFCRQGFPHTSMDEVAKQAGVSKQTVYSHYGSKDDLFVAAIE--S 68
+++ +Q +L A+ LF +QG TS+ E+AK AGV++ +Y H+ K DLF E
Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67

Query: 69 KCVGHNLNADLLSNPSQPEATLTEFALQFGEMIVSPEAITVFKACVAQSESHP---EVSR 125
+G P P + L E + E V+ E + + V +
Sbjct: 68 SNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQ 127

Query: 126 LFFEAGPQHMLAMLTKYLGAVEALGVYRFSQPHHCAVRLCLMLFGELKLRLELGLETESL 185
+ + L + A + L ++ L
Sbjct: 128 QAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDL 187

Query: 186 LGEREQYIRGCAEMFLK 202
E Y+ EM+L
Sbjct: 188 KKEARDYVAILLEMYLL 204


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1301BONTOXILYSIN330.007 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 32.6 bits (74), Expect = 0.007
Identities = 24/159 (15%), Positives = 56/159 (35%), Gaps = 11/159 (6%)

Query: 418 EITLPPLEKSKLLSDSQFNTIKENDLNKAYFECKKIRNELIKLQY---LITEAKKLASKL 474
+ P +E + + K DLN +E K Y L + S+
Sbjct: 623 NLREPNIEIDDISDSLLGLSFK--DLNNKLYEIYSKNIVYFKKIYFSFLDQWWTEYYSQY 680

Query: 475 NLDSKKSSSTLE-KIDKIELKINKKYKNLSQLIKFYGYFEFSKFLTT---REIESWTQPQ 530
+ ++ + ++ + K+ +LS+ + + T ++ + +Q
Sbjct: 681 FELICMAKQSILAQESLVKQIVQNKFTDLSKASIPPDTLKLIRETTEKTFIDLSNESQIS 740

Query: 531 INQMTERYYEAFLSSCVQVFKLVESACNKLQDRINELKT 569
+N++ +A S CV V + + ++ IN +
Sbjct: 741 MNRVDNFLNKA--SICVFVEDIYPKFISYMEKYINNINI 777


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1302NUCEPIMERASE761e-17 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 75.6 bits (186), Expect = 1e-17
Identities = 41/231 (17%), Positives = 78/231 (33%), Gaps = 54/231 (23%)

Query: 6 TILITGGTGSFGQKYTKTILERY-----------------KPKRLIIFSRDELKQYEMQQ 48
L+TG G G +K +LE K RL + ++ + ++
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHK--- 58

Query: 49 VFNAPCMRYFIGDVRDGDRLKQAFKDVDF--VIHAAALKQVPAAEYNPMECIKTNIHGAE 106
D+ D + + F F V + V + NP +N+ G
Sbjct: 59 -----------IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFL 107

Query: 107 NVIRAAISNNVVKVIALST---------------DKAASPINLYGATKLASDKLFVAANN 151
N++ N + ++ S+ D P++LY ATK A++ + ++
Sbjct: 108 NILEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH 167

Query: 152 VVGDGKTRFAAVRYGNVVGSRGS---VVPFFKQLIANGATSLPITHPDMTR 199
+ G +R+ V G G + F + + G + + M R
Sbjct: 168 LYG---LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKR 215


70Shewana3_1319Shewana3_1363N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1319022-3.990028response regulator receiver modulated CheW
Shewana3_1320023-3.113480chemotaxis protein CheR
Shewana3_1321223-2.880900flagellar basal body rod protein FlgB
Shewana3_1322223-2.856214flagellar basal body rod protein FlgC
Shewana3_1323224-2.952221flagellar basal body rod modification protein
Shewana3_1324123-2.472489flagellar hook protein FlgE
Shewana3_1325-125-2.989144flagellar basal body rod protein FlgF
Shewana3_1326-126-3.846569flagellar basal body rod protein FlgG
Shewana3_1327-129-4.367629flagellar basal body L-ring protein
Shewana3_1328131-4.818899flagellar basal body P-ring protein
Shewana3_1329131-4.988498flagellar rod assembly protein/muramidase FlgJ
Shewana3_1330236-6.361759flagellar hook-associated protein FlgK
Shewana3_1331637-7.146169flagellar hook-associated protein FlgL
Shewana3_1332640-7.849937flagellin domain-containing protein
Shewana3_1333435-6.618565flagellin domain-containing protein
Shewana3_1334330-5.198591flagellar protein FlaG protein
Shewana3_1335125-4.259888flagellar hook-associated 2 domain-containing
Shewana3_1336119-2.866022hypothetical protein
Shewana3_1337017-1.755837flagellar protein FliS
Shewana3_1338-114-1.131923sigma-54 dependent trancsriptional regulator
Shewana3_1339011-0.327079PAS/PAC sensor signal transduction histidine
Shewana3_1340-1110.798913two component, sigma54 specific, Fis family
Shewana3_13410100.991372flagellar hook-basal body complex subunit FliE
Shewana3_13420120.798264flagellar MS-ring protein
Shewana3_13431120.706761flagellar motor switch protein G
Shewana3_13440120.846923flagellar assembly protein H
Shewana3_13450120.567590flagellum-specific ATP synthase
Shewana3_1346015-1.000389flagellar export protein FliJ
Shewana3_1347-115-1.090176flagellar hook-length control protein
Shewana3_1348-117-1.271387flagellar basal body-associated protein FliL
Shewana3_1349015-1.260481flagellar motor switch protein FliM
Shewana3_1350-117-0.707323flagellar motor switch protein
Shewana3_1351-115-0.670210flagellar biosynthesis protein, FliO
Shewana3_1352-213-0.567427flagellar biosynthesis protein FliP
Shewana3_1353-213-0.529836flagellar biosynthetic protein FliQ
Shewana3_1354-113-0.854080flagellar biosynthesis protein FliR
Shewana3_1355-114-1.100185flagellar biosynthesis protein FlhB
Shewana3_1356017-1.847798flagellar biosynthesis protein FlhA
Shewana3_1357118-1.367360flagellar biosynthesis regulator FlhF
Shewana3_1358017-0.807742cobyrinic acid a,c-diamide synthase
Shewana3_1359119-0.450930flagellar biosynthesis sigma factor
Shewana3_1360017-0.006862response regulator receiver protein
Shewana3_1361018-0.136532chemotaxis phosphatase, CheZ
Shewana3_1362-1170.087767CheA signal transduction histidine kinase
Shewana3_1363-321-0.328434chemotaxis-specific methylesterase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1319HTHFIS633e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.9 bits (153), Expect = 3e-13
Identities = 25/128 (19%), Positives = 53/128 (41%), Gaps = 12/128 (9%)

Query: 180 HIMVIDDSAVARKQIIRALESLNLQIDTAKDGREALDKLKAIAGEMNNVAEEIPLIISDI 239
I+V DD A R + +AL + + + A G+ L+++D+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGD---------LVVTDV 55

Query: 240 EMPEMDGYTLTAEIRDDPKLKHIKVVLHTSLSGVFNQAMVQKVGANDFIAK-FNPDELAA 298
MP+ + + L I+ + V++ ++ + + GA D++ K F+ EL
Sbjct: 56 VMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG 113

Query: 299 AVNKHLSL 306
+ + L+
Sbjct: 114 IIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1322FLGHOOKAP1333e-04 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 32.6 bits (74), Expect = 3e-04
Identities = 9/38 (23%), Positives = 18/38 (47%)

Query: 99 NVNVMEEMADMISASRSYQMNVQVAEAAKSMLQQTLGM 136
VN+ EE ++ + Y N QV + A ++ + +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 28.4 bits (63), Expect = 0.011
Identities = 15/64 (23%), Positives = 27/64 (42%), Gaps = 6/64 (9%)

Query: 8 DVAGSGMSAQSLRLNTTASNIANADSVSSSVDKTYRSRHPIFEAEMAKAQSQQQASQGVT 67
+ A SG++A LNT ++NI++ + Y + I + + GV
Sbjct: 5 NNAMSGLNAAQAALNTASNNISSYN------VAGYTRQTTIMAQANSTLGAGGWVGNGVY 58

Query: 68 VKGI 71
V G+
Sbjct: 59 VSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1324FLGHOOKAP1401e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 40.3 bits (94), Expect = 1e-05
Identities = 15/35 (42%), Positives = 22/35 (62%)

Query: 2 SFNIALSGISAAQKDLNTTANNIANANTIGFKESR 36
N A+SG++AAQ LNT +NNI++ N G+
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37



Score = 37.2 bits (86), Expect = 1e-04
Identities = 13/49 (26%), Positives = 25/49 (51%)

Query: 405 SLSSSALEQSNIDLTTELVDLISAQRNFQANSRTLEVNNTLQQTVLQIR 453
LS+ S ++L E +L Q+ + AN++ L+ N + ++ IR
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1326FLGHOOKAP1452e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.6 bits (105), Expect = 2e-07
Identities = 19/119 (15%), Positives = 41/119 (34%), Gaps = 4/119 (3%)

Query: 145 EDATSITVSAEGEVSVKTPGAAENQVVGQLSMTDFINPSGLDPMGQNLYTETG---ASGT 201
D I +++E + + + Q + + +L ++ G A+
Sbjct: 427 TDEAKIAMASEEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLK 486

Query: 202 PIQGTASLDGMGAIRQGALETSNVNVTEELVNLIESQRIYEMNSKVISAVDQMLAYVNQ 260
T + + S VN+ EE NL Q+ Y N++V+ + + +
Sbjct: 487 TSSATQGNV-VTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544



Score = 35.7 bits (82), Expect = 2e-04
Identities = 9/36 (25%), Positives = 20/36 (55%)

Query: 5 LWISKTGLDAQQTDIAVISNNVANASTVGYKKSRAV 40
+ + +GL+A Q + SNN+++ + GY + +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI 39


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1327FLGLRINGFLGH1472e-46 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 147 bits (373), Expect = 2e-46
Identities = 75/215 (34%), Positives = 106/215 (49%), Gaps = 9/215 (4%)

Query: 11 LLLAACSSTPKKPIADDPFYAPVYPEAPPTKIAATGSIYQDSQAA-----SLYSDIRAHK 65
L L C+ P P+ A P P A GSI+Q +Q L+ D R
Sbjct: 17 LSLTGCAWIPSTPLVQGATSAQPVPGPTP---VANGSIFQSAQPINYGYQPLFEDRRPRN 73

Query: 66 VGDIITIVLKEATQAKKSAGNQIKKGSDMTLDPIYAGGSNVSL-GGIPLDLRYKDSMNTK 124
+GD +TIVL+E A KS+ + L G D+
Sbjct: 74 IGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGGNTFN 133

Query: 125 RESDADQSNSLDGSISANIMQVLNNGNLVVRGEKWISINNGDEFIRVTGIVRSQDIKPDN 184
+ A+ SN+ G+++ + QVL NGNL V GEK I+IN G EFIR +G+V + I N
Sbjct: 134 GKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTISGSN 193

Query: 185 TIDSTRMANARIQYSGTGTFAEAQKVGWLSQFFMS 219
T+ ST++A+ARI+Y G G EAQ +GWL +FF++
Sbjct: 194 TVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLN 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1328FLGPRINGFLGI370e-130 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 370 bits (952), Expect = e-130
Identities = 157/367 (42%), Positives = 222/367 (60%), Gaps = 14/367 (3%)

Query: 5 LIVALAMLVLSLPSQAE--RIKDIANVQGVRSNQLIGYGLVVGLPGTGEK---TNYTEQT 59
L+ + + + P+QA+ RIKDIA++Q R NQLIGYGLVVGL GTG+ + +TEQ+
Sbjct: 11 LVFSALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQS 70

Query: 60 FTTMLKNFGINLPDNFRPKIKNVAVVAVHADMPAFIKPGQQLDVTVSSLGEAKSLRGGTL 119
ML+N GI + KN+A V V A++P F PG ++DVTVSSLG+A SLRGG L
Sbjct: 71 MRAMLQNLGITTQGG-QSNAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNL 129

Query: 120 LQTFLKGVDGNVYAIAQGSLVVSGFSAEGLDGSKVIQNTPTVGRIPNGAIVERSVATPFS 179
+ T L G DG +YA+AQG+L+V+GFSA+G D + + Q T R+PNGAI+ER + + F
Sbjct: 130 IMTSLSGADGQIYAVAQGALIVNGFSAQG-DAATLTQGVTTSARVPNGAIIERELPSKFK 188

Query: 180 SGDYLTFNLRRADFSTAQRMADAINDL----LGPDMARPLDATSVQVSAPRDVSQRVSFL 235
L LR DFSTA R+AD +N G +A P D+ + V PR V+ +
Sbjct: 189 DSVNLVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPR-VADLTRLM 247

Query: 236 ATLENLDVIPAEESAKVIVNSRTGTIVVGQHVKLLPAAVTHGGLTVTIAEATQVSQPNAL 295
A +ENL + + AKV++N RTGTIV+G V++ AV++G LTV + E+ QV QP
Sbjct: 248 AEIENL-TVETDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPF 306

Query: 296 ANGETVVTANTTIGVNESDRRMFMFSPGTTLDELVRAVNLVGAAPSDVLAILEALKVAGA 355
+ G+T V T I + ++ G L LV +N +G ++AIL+ +K AGA
Sbjct: 307 SRGQTAVQPQTDIMAMQEGSKVA-IVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGA 365

Query: 356 LHGELII 362
L EL++
Sbjct: 366 LQAELVL 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1329FLGFLGJ1492e-44 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 149 bits (378), Expect = 2e-44
Identities = 67/160 (41%), Positives = 95/160 (59%), Gaps = 2/160 (1%)

Query: 219 RETQKTLKFGSREEFLATLYPHAEKAAKALGTQPEVLLAQSALETGWGQKIVRGSNGAPS 278
R +L S+ FLA L A+ A++ G ++LAQ+ALE+GWGQ+ +R NG PS
Sbjct: 139 RNYDDSLPGDSKA-FLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPS 197

Query: 279 HNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDFEHSFNDFVTFIAEGERYQ 338
+NLF +KA W G ++T E+E G A + KA FRVY+ + + +D+V + RY
Sbjct: 198 YNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYA 257

Query: 339 DAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQTISQ 378
A AAS Q +ALQDAGYATDP YA K+ ++Q +
Sbjct: 258 -AVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKS 296



Score = 87.8 bits (217), Expect = 1e-21
Identities = 39/93 (41%), Positives = 61/93 (65%), Gaps = 3/93 (3%)

Query: 12 DLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFY 71
D L+ L+A+A +D ++ VA+Q EG+FVQM++KSMRDA D +S++T+ Y
Sbjct: 14 DAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALP---KDGLFSSEHTRLY 70

Query: 72 EQMRDQQLSVDLSDKGVLGLADMMVQQLSPESS 104
M DQQ++ ++ LGLA+MMV+Q++PE
Sbjct: 71 TSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQP 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1330FLGHOOKAP12196e-66 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 219 bits (560), Expect = 6e-66
Identities = 127/460 (27%), Positives = 193/460 (41%), Gaps = 29/460 (6%)

Query: 4 DLLNIARTGVLASQSQLGVTSNNIANANTAGYHRQVATQSTLESQRLGNSFYGTGTYVDD 63
L+N A +G+ A+Q+ L SNNI++ N AGY RQ + S + G G YV
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSG 61

Query: 64 VKRIYNDYAARELRIGQTTLSGAEASYGKLSELDQLFSQIGKMVPQSLNSLFTGLNSLAD 123
V+R Y+ + +LR QT SG A Y ++S++D + S + + FT L +L
Sbjct: 62 VQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVS 121

Query: 124 LPADLGIRSSTLNDAKQLANSLNQMQSSLNGQMTQTNDQITGMTKRINEISKELANLNLE 183
D R + + ++ L N L Q Q N I +IN +K++A+LN +
Sbjct: 122 NAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLNDQ 181

Query: 184 LMKSPNQDAM-----LLDKQDALIQELSQYAQVNVIPQENGAKSIMLGGSVMLVSGEIAM 238
+ + A LLD++D L+ EL+Q V V Q+ G +I + LV G A
Sbjct: 182 ISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTAR 241

Query: 239 TMDTKTGDPFPNELQLTSSIGSQSVAADPSKL--GGQLGALFEYRDQTLIPASHELDQLA 296
+ P+ + G+ P KL G LG + +R Q L + L QLA
Sbjct: 242 QLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLA 301

Query: 297 LGIADNFNKMQAQGFDLNGQVGSNIFRDINDPLMSLGRVGGYSNNTGNATLGVNIDDTRL 356
L A+ FN GFD NG G + F + V + N G+ +G + D
Sbjct: 302 LAFAEAFNTQHKAGFDANGDAGEDFFA------IGKPAVLQNTKNKGDVAIGATVTDASA 355

Query: 357 LTGGSYELSF-------TAPASYELRDTETGVITPLTLNGSTLEGGAGFSIDIKAGAMAS 409
+ Y++SF T AS + +G L G A
Sbjct: 356 VLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFT---------GTPAV 406

Query: 410 GDRFVIRPTAGAANGITVEMTDPKGIAAASPKITADAANS 449
D F ++P + A + V +TD IA AS + D+ N
Sbjct: 407 NDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNR 446



Score = 89.6 bits (222), Expect = 8e-21
Identities = 38/103 (36%), Positives = 55/103 (53%)

Query: 535 AEGDNSNAVAMAKLSESKVMNGGKSTLADVFENTKIDIGSKTKAAEVRVGSAEAIYQQAY 594
+ DN N A+ L + GG + D + + DIG+KT + + + Q
Sbjct: 441 GDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLS 500

Query: 595 ARVESESGVNLDEEAANLMRFQQAYQASARIMTTAQQIFDTLL 637
+ +S SGVNLDEE NL RFQQ Y A+A+++ TA IFD L+
Sbjct: 501 NQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALI 543


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1331FLAGELLIN582e-11 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 58.1 bits (140), Expect = 2e-11
Identities = 63/359 (17%), Positives = 119/359 (33%), Gaps = 8/359 (2%)

Query: 20 QTATSKILEQLSSGKKVNTAGDDPVAALGIDNLNQRNALVDQFMKNIDYATNRLAVTESK 79
Q++ S +E+LSSG ++N+A DD + + Q +N + + TE
Sbjct: 21 QSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNANDGISIAQTTEGA 80

Query: 80 LGSAENLASSIREQVMRAVNGTLADSERQMIADEMKGSLEELLSIANSKDESGNYMFSGY 139
L N +RE ++A NGT +DS+ + I DE++ LEE+ ++N +G + S
Sbjct: 81 LNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKVLSQ- 139

Query: 140 STDKEPFAFDNSTPPKIVYSGDSGIRNSLVQSGVALGTNVPGDTAFMKAPNGLGDYSVNY 199
+ N + ++ V+S G NV G +V
Sbjct: 140 DNQMKIQVGANDGETITID-----LQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTG 194

Query: 200 LASQQGEFSVKTAKIADPATYLADTYTFNFSDNGSGGTNLQVLDSANNPVANIANFDAAT 259
+ + + A T N Q+ + F
Sbjct: 195 YDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTK 254

Query: 260 PVSFNGIEVNISGKPSAGDSFTMEPQSEVSIFDTISRAIALIEDPNSANTPQGRSQLAQI 319
+ I+G G V+ TI + + T G +
Sbjct: 255 STAGTAEAKAIAGAIKGGKEGDTFDYKGVTF--TIDTKTGNDGNGKVSTTINGEKVTLTV 312

Query: 320 LNDIDSGVNQISSARSVAGNNLKAVESYKDTHIEEQVLNTSALSLLEDLDYASAITEFA 378
+ N ++ + N +V + + T ++ ++ LS LE + ++
Sbjct: 313 ADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKIT 371


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1332FLAGELLIN1344e-38 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 134 bits (337), Expect = 4e-38
Identities = 93/271 (34%), Positives = 125/271 (46%), Gaps = 10/271 (3%)

Query: 2 AITVNTNVTSMKAQKNLNTSSSGLATSMERLSSGLRINSAKDDAAGLAISNRLNSQVRGL 61
A +NTN S+ Q NLN S S L++++ERLSSGLRINSAKDDAAG AI+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 DVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTVQAENGANSTDDLDAIQKEINQLSE 121
RNAND ISIAQ EGA+ E N LQR+R+L+VQA NG NS DL +IQ EI Q E
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EITAIGDSTAFGNTLLMTGLFSTGKTFQVGHQEGEDITISVGTTNAGSL--------SVN 173
EI + + T F +++ QVG +GE ITI + + SL
Sbjct: 121 EIDRVSNQTQFNGVKVLSQ--DNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPK 178

Query: 174 ALAIASAGGRSTALANIDAAIKTIDNQRANLGAKQNRLAYNISNSANTQANVADAKSRIV 233
+ + D + R ++ + + A
Sbjct: 179 EATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTT 238

Query: 234 DVDFAKETSVMTKNQVLQQTGSAMLAQANQL 264
D + K + A A +
Sbjct: 239 DDAENNTAVDLFKTTKSTAGTAEAKAIAGAI 269



Score = 85.9 bits (212), Expect = 4e-21
Identities = 64/213 (30%), Positives = 99/213 (46%), Gaps = 4/213 (1%)

Query: 60 GLDVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTVQAENGANSTDDLDAIQKEINQL 119
+ + +++A I GA LQ +++ NG + DD +
Sbjct: 298 KVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSD 357

Query: 120 SEEITAIGDSTAFGNTLLMTGLFSTGKTFQVGHQEGEDITISVGTTNAGSLSVNALAIAS 179
E A+ + + G + + T + S +N A A+
Sbjct: 358 LEANNAVKGESKITVNGAEYTANAAGDKVTLAGK----TMFIDKTASGVSTLINEDAAAA 413

Query: 180 AGGRSTALANIDAAIKTIDNQRANLGAKQNRLAYNISNSANTQANVADAKSRIVDVDFAK 239
+ LA+ID+A+ +D R++LGA QNR I+N NT N+ A+SRI D D+A
Sbjct: 414 KKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYAT 473

Query: 240 ETSVMTKNQVLQQTGSAMLAQANQLPQVALSLL 272
E S M+K Q+LQQ G+++LAQANQ+PQ LSLL
Sbjct: 474 EVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1333FLAGELLIN1395e-40 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 139 bits (350), Expect = 5e-40
Identities = 98/270 (36%), Positives = 129/270 (47%), Gaps = 10/270 (3%)

Query: 2 AITVNTNVTSLKAQKNLNTSASGLATSMERLSSGLRINSAKDDAAGLAISNRLNSQVRGL 61
A +NTN SL Q NLN S S L++++ERLSSGLRINSAKDDAAG AI+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 EVGMRNANDAISIAQISEGAMQEQTNMLQRMRDLTVQSENGANSTDDLDAIQKEIDQLAL 121
RNAND ISIAQ +EGA+ E N LQR+R+L+VQ+ NG NS DL +IQ EI Q
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EITEIGDNTAFGSTKLLDGTFSGKTFQVGHQSGEDITISVAKTTASALKVDSLDITGSAR 181
EI + + T F K+L QVG GE ITI + K +L +D ++ G
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQ-MKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 182 ASALAA---------IDAAIKTIDSQRADLGAKQNRLAYNISNSANTQANIADAKSRIVD 232
A+ D + R D+ + + A D
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 233 VDFAKETSQMTKNQVLQQTGSAMLAQANQL 262
+ K + A A +
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAI 269



Score = 84.3 bits (208), Expect = 1e-20
Identities = 63/212 (29%), Positives = 101/212 (47%), Gaps = 4/212 (1%)

Query: 60 GLEVGMRNANDAISIAQISEGAMQEQTNMLQRMRDLTVQSENGANSTDDLDAIQKEIDQ- 118
+ + +++A I+ GA LQ +++ NG + DD +
Sbjct: 298 KVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSD 357

Query: 119 LALEITEIGDNTAFGSTKLLDGTFSGKTFQVGHQSGEDITISVAKTTASALKVDSLDITG 178
L G++ + +G + ++ I + S L +
Sbjct: 358 LEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTM---FIDKTASGVSTLINEDAAAAK 414

Query: 179 SARASALAAIDAAIKTIDSQRADLGAKQNRLAYNISNSANTQANIADAKSRIVDVDFAKE 238
+ A+ LA+ID+A+ +D+ R+ LGA QNR I+N NT N+ A+SRI D D+A E
Sbjct: 415 KSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATE 474

Query: 239 TSQMTKNQVLQQTGSAMLAQANQLPQVALSLL 270
S M+K Q+LQQ G+++LAQANQ+PQ LSLL
Sbjct: 475 VSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1338HTHFIS452e-158 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 452 bits (1164), Expect = e-158
Identities = 172/479 (35%), Positives = 263/479 (54%), Gaps = 17/479 (3%)

Query: 7 RILLVGTPSERLSRLCCIFEFLGEQIEIVAIEKLSLCLQDTRFRALVVTADNMP----AD 62
IL+ + + L G + I + LVVT MP D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 ALKSLASQYPWQPILL---FGNVDDLQVSNVLG---HIEEPLNYPQLTELLHFCQVYGQV 116
L + P P+L+ ++ G ++ +P + +L ++ +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 117 KRPQVPTSANQTKLFRSLVGRSEGIANVRHLISQVATSDATVLVLGQSGTGKEVVARNIH 176
+ ++ + LVGRS + + +++++ +D T+++ G+SGTGKE+VAR +H
Sbjct: 125 RPSKLEDDSQD---GMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALH 181

Query: 177 YLSERRDGPFIPVNCGAIPPELLESELFGHEKGSFTGAISSRKGRFELAEGGTLFLDEIG 236
+RR+GPF+ +N AIP +L+ESELFGHEKG+FTGA + GRFE AEGGTLFLDEIG
Sbjct: 182 DYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG 241

Query: 237 DMPLQMQVKLLRVLQERVFERVGGTKTINADVRVVAATHRDLETMISSNEFREDLYYRLN 296
DMP+ Q +LLRVLQ+ + VGG I +DVR+VAAT++DL+ I+ FREDLYYRLN
Sbjct: 242 DMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLN 301

Query: 297 VFPIEMPALSERKDDVPLLLQELVSRVYNEGRGKVRFTQRAIESLKEHAWSGNVRELSNL 356
V P+ +P L +R +D+P L++ V + EG RF Q A+E +K H W GNVREL NL
Sbjct: 302 VVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENL 361

Query: 357 VERLTILYPGGLVDVNDLPVKYRHIDVPEYCVEMSEEQQERDALASIFTSEEPVEIPETR 416
V RLT LYP ++ + + R ++P+ +E + + +++ EE +
Sbjct: 362 VRRLTALYPQDVITREIIENELRS-EIPDSPIEKAAARSGSLSISQAV--EENMRQYFAS 418

Query: 417 FPSELPPEGVNLKDLLAELEIDMIRQALELQDNVVARAAEMLGIRRTTLVEKMRKYGMT 475
F LPP G+ +LAE+E +I AL +AA++LG+ R TL +K+R+ G++
Sbjct: 419 FGDALPPSGL-YDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1339PF06580290.033 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 28.7 bits (64), Expect = 0.033
Identities = 26/145 (17%), Positives = 50/145 (34%), Gaps = 22/145 (15%)

Query: 205 GELINLNDVIENVVANCEPIVAKHGAELAVT-NISNSLMLANVNALSSAVNNLVMNSLEA 263
++L D + V + + + L I+ ++M V + V LV N ++
Sbjct: 213 ARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPML--VQTLVENGIKH 270

Query: 264 GATQ------IQIVASDSNEQLHLNVIDNGKGLDAKMQQKVLEPFFTTKAQGTGLGLA-V 316
G Q I + + N + L V + G + TG GL V
Sbjct: 271 GIAQLPQGGKILLKGTKDNGTVTLEVENTGSL------------ALKNTKESTGTGLQNV 318

Query: 317 VQSVVRNHGGQLQLSCMPNKGCTVS 341
+ + +G + Q+ +G +
Sbjct: 319 RERLQMLYGTEAQIKLSEKQGKVNA 343


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1340HTHFIS465e-164 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 465 bits (1199), Expect = e-164
Identities = 171/483 (35%), Positives = 249/483 (51%), Gaps = 43/483 (8%)

Query: 1 MSEAKLLLVEDDASLREALLDTLMLAQYDCIDVASGEEAIIVLKQHQFDLVISDVQMQGI 60
M+ A +L+ +DDA++R L L A YD ++ + DLV++DV M
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 GGLGLLNYLQQHHPKLPVLLMTAYATIGSAVSAIKLGAVDYLAKPFAPEVLLNQVSRYLP 120
LL +++ P LPVL+M+A T +A+ A + GA DYL KPF L+ + R L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 LKQNSDQPVVAD-----------EKSLALLSLAQRVAASDASVMILGPSGSGKEVLARYI 169
+ + D + + R+ +D ++MI G SG+GKE++AR +
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180

Query: 170 HQHSSRADEAFVAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEI 229
H + R + FVAIN AAIP +++E+ LFG+EKGAFTGA G+FEQA+GGTL LDEI
Sbjct: 181 HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI 240

Query: 230 SEMDLGLQAKLLRVLQEREVERLGGRKTIKLDVRVLATSNRDLKAVVAAGQFREDLYYRI 289
+M + Q +LLRVLQ+ E +GGR I+ DVR++A +N+DLK + G FREDLYYR+
Sbjct: 241 GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300

Query: 290 NVFPLTWPALNQRPADILPLARHLLTKHAKALSIIDVPEFDEAACRRLLGHRWPGNVREL 349
NV PL P L R DI L RH + + K +DV FD+ A + H WPGNVREL
Sbjct: 301 NVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEG--LDVKRFDQEALELMKAHPWPGNVREL 358

Query: 350 DNVVQRALILRSGTVITANDIIIDAQDVPLSSDD-------------------------- 383
+N+V+R L VIT I + + S
Sbjct: 359 ENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFAS 418

Query: 384 -AEYCSEPEGLGEELKAQEHVIILETLAQCQGSRKLVAEKLGISARTLRYKMARMRDMGI 442
+ L E+ +IL L +G++ A+ LG++ TLR K +R++G+
Sbjct: 419 FGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKK---IRELGV 475

Query: 443 QLP 445
+
Sbjct: 476 SVY 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1341FLGHOOKFLIE591e-14 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 58.5 bits (141), Expect = 1e-14
Identities = 31/101 (30%), Positives = 54/101 (53%)

Query: 12 MQSLQGEIKPSFGISPNNIVQQVNNTSGADFGQLLSQAIGNVSGLQSTSSNLSTRLEMGD 71
+Q ++G I + + Q+ F L A+ +S Q+ + + + +G+
Sbjct: 3 IQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGE 62

Query: 72 TTVSLSDTVIAREKASVAFEATVQVRNKLVEAYKEIMSMPV 112
V+L+D + +KASV+ + +QVRNKLV AY+E+MSM V
Sbjct: 63 PGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1342FLGMRINGFLIF2991e-96 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 299 bits (766), Expect = 1e-96
Identities = 158/560 (28%), Positives = 270/560 (48%), Gaps = 43/560 (7%)

Query: 26 LGGVDMMRQITMILALAICLALAVFVMIWAQEPEYRPL-GKMETQEMVQVLDVLDKNKIK 84
L + +I +I+A + +A+ V +++WA+ P+YR L + Q+ ++ L + I
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 85 YQIEVD--VVKVPEDKYQEVKMMLSRAGVNSPAASTQDFLTQDSGFGVSQRMEQARLKHS 142
Y+ ++VP DK E+++ L++ G+ A + L Q FG+SQ EQ + +
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQ-EKFGISQFSEQVNYQRA 134

Query: 143 QEENLARAIEQLQSVSRAKVILALPKENVFARNTSQPSATVVINTRRG-GLGQGEVDAIV 201
E LAR IE L V A+V LA+PK ++F R PSA+V + G L +G++ A+V
Sbjct: 135 LEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVV 194

Query: 202 DIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGISARARRELELVQQKEAEYRTKIDSILS 261
+V+SAV GL P VT+ D +G LL + G +L+ E+ + +I++ILS
Sbjct: 195 HLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDL-NDAQLKFANDVESRIQRRIEAILS 253

Query: 262 PILGPDNFTSQVDVSMDFTAVEQTAKRFNPDLPSLRSEMTVENNST-----GGSTGGIPG 316
PI+G N +QV +DF EQT + ++P+ + ++ + + G GG+PG
Sbjct: 254 PIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPG 313

Query: 317 ALSNQPP---------------MESNIPQDAT-KATESVTAGNSHREATRNFELDTTISH 360
ALSNQP N PQ +T + S ++ R T N+E+D TI H
Sbjct: 314 ALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRH 373

Query: 361 TRQQIGVVRRVSVSVAVDFKPGAAGENGQVARVARTEQELTNIRRLLEGAVGFSAQRGDV 420
T+ +G + R+SV+V V++K A G+ + T ++ I L A+GFS +RGD
Sbjct: 374 TKMNVGDIERLSVAVVVNYKTLADGKP-----LPLTADQMKQIEDLTREAMGFSDKRGDT 428

Query: 421 LEVVTVPFMDQLVEDVPAPELWEQPWFWRAVKLGIGALVILVLILAVVRPMLKRLIYPDS 480
L VV PF + W+Q F + L L+L V + ++ + P
Sbjct: 429 LNVVNSPF-SAVDNTGGELPFWQQQSFIDQLLAAGRWL----LVLVVAWILWRKAVRPQL 483

Query: 481 VNMPEDSRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSILIPNLHKDDDMIKAIRA 540
E+++ E A++ + L+ + E + + + M + IR
Sbjct: 484 TRRVEEAKAAQEQAQVRQETEEAVEVRLS--KDEQLQQRRANQRLGA----EVMSQRIRE 537

Query: 541 LVANEPELSTQVVKNWLQDN 560
+ N+P + V++ W+ ++
Sbjct: 538 MSDNDPRVVALVIRQWMSND 557


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1343FLGMOTORFLIG2904e-99 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 290 bits (745), Expect = 4e-99
Identities = 109/348 (31%), Positives = 194/348 (55%), Gaps = 5/348 (1%)

Query: 1 MAENKSKDAAETSSFNIKDLSGIEKTAILLLSLSEADAASILKHLEPKQVQKVGMAMAAM 60
M E K K+ + + L+G +K AILL+S+ ++ + K+L ++++ + +A +
Sbjct: 1 MEEKKEKEILD-----VSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKL 55

Query: 61 EDFGQEKVIGVHKLFLDDIQKYSSIGFNSEEFVRKALTAALGEDKAGNLIEQIIMGSGAK 120
E E V F + + I ++ R+ L +LG KA ++I + ++
Sbjct: 56 ETITSELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSR 115

Query: 121 GLDSLKWMDARQVATIIQNEHPQIQTIVLSYLEPDQAAEIFGQFPENTRLDLMMRIANLE 180
+ ++ D + IQ EHPQ ++LSYL+P +A+ I P + ++ RIA ++
Sbjct: 116 PFEFVRRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMD 175

Query: 181 EVQPAALQELNDIMEKQFAGQGGAQAAKMGGLKAAANIMNYLDTGVESQLMETMRETDEE 240
P ++E+ ++EK+ A GG+ I+N D E ++E++ E D E
Sbjct: 176 RTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPE 235

Query: 241 MAQQIQDLMFVFENLIDVDDRGIQTLLREVQQDVLMKALKGADDQLKDKILGNMSKRAAE 300
+A++I+ MFVFE+++ +DDR IQ +LRE+ L KALK D +++KI NMSKRAA
Sbjct: 236 LAEEIKKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAAS 295

Query: 301 LLRDDLEAMGPIRISEVEIAQKEILSIARRLSDSGEIMLGGGGGDEFL 348
+L++D+E +GP R +VE +Q++I+S+ R+L + GEI++ GG ++ L
Sbjct: 296 MLKEDMEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVL 343


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1344FLGFLIH918e-24 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 91.4 bits (226), Expect = 8e-24
Identities = 59/203 (29%), Positives = 106/203 (52%), Gaps = 9/203 (4%)

Query: 48 YSPQQAPKAVAAETIAPPTMAEIEDIRAQAEEEGFNEGKTQGYAEGLEQGRLEGLEQGHT 107
+ P P+ E P ++ ++ QA E QGY G+ +GR +G +QG+
Sbjct: 22 FVPIVEPEETIIEEAEPSLEQQLAQLQMQAHE--------QGYQAGIAEGRQQGHKQGYQ 73

Query: 108 EGLAQGHEQGLEAGLAEAKALVSRFEGLLSQFEKPLQLLDGDIEHSLMTLTMALAKSVIG 167
EGLAQG EQGL ++ + +R + L+S+F+ L LD I LM + + A+ VIG
Sbjct: 74 EGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIG 133

Query: 168 HELKTHPEQILSALRLGVESLPIKEQSVSIRMHPDDVALVEQLYSSTQLNRNQWQLEADP 227
++ ++ ++ P+ +R+HPDD+ V+ + +T L+ + W+L DP
Sbjct: 134 QTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT-LSLHGWRLRGDP 192

Query: 228 SLNSGDCIISSQRSLVDLTLSSR 250
+L+ G C +S+ +D ++++R
Sbjct: 193 TLHPGGCKVSADEGDLDASVATR 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1346FLGFLIJ442e-08 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 44.0 bits (103), Expect = 2e-08
Identities = 37/145 (25%), Positives = 71/145 (48%)

Query: 1 MANADPLLLVLKLALDAEEQAALLLKSAQLECQKRQNQLDALNNYRLDYMKQMQSQQGQA 60
MA L + LA E AA LL + CQ+ + QL L +Y+ +Y + S
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 ISASHYHQFHRFIRQIDEAIAQQNRVVADGEKQKNYRQQHWLEKQKKRKAVELLLDNKEK 120
I+++ + + +FI+ +++AI Q + + ++ + W EK+++ +A + L + +
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 KRQALELKKEQKMTDEFASQQFFRR 145
E + +QK DEFA + R+
Sbjct: 121 AALLAENRLDQKKMDEFAQRAAMRK 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1347FLGHOOKFLIK548e-10 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 53.7 bits (128), Expect = 8e-10
Identities = 49/198 (24%), Positives = 83/198 (41%), Gaps = 17/198 (8%)

Query: 387 VAALSSGSEEADSEFKPVEFKAVPSLHSLATPATQRQDIPQVQLSLRQGVETQNQMQEMI 446
+ A + E S PV A P + Q Q +P V + ++ Q+
Sbjct: 190 LVAEAQSKAEVISTPSPVTAAASPLITP-----HQTQPLPTVAAPVLSAPLGSHEWQQ-- 242

Query: 447 QRFSPVMKQQLVTMVSQGIQQAEIRLDPPELGHMLVKVQVHGDQTQVQFHVTQSQTRDVV 506
+ Q + QG Q AE+RL P +LG + + ++V +Q Q+Q R +
Sbjct: 243 -----SLSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAAL 297

Query: 507 EQAIPRLRELLQEQGMQLADSHVSQGDHGQRREGGFGEAGGSSGGNVDDFSAEELD---- 562
E A+P LR L E G+QL S++S +++ + N + + E+ D
Sbjct: 298 EAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPV 357

Query: 563 -LGLNQATSLNSGIDYYA 579
+ L + NSG+D +A
Sbjct: 358 PVSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1349FLGMOTORFLIM2497e-83 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 249 bits (636), Expect = 7e-83
Identities = 88/327 (26%), Positives = 165/327 (50%), Gaps = 12/327 (3%)

Query: 1 MSDLLSQDEIDALLHGVDDVEEDNELDAAGLEARS----YDFSSQDRIVRGRMPTLEIVN 56
M+++LSQDEID LL + + E DA + YDF D+ + +M TL +++
Sbjct: 1 MTEVLSQDEIDQLLTAISSGDASIE-DARPISDTRKITLYDFRRPDKFSKEQMRTLSLMH 59

Query: 57 ERFARHLRISMFNMMRRAAEVSINGVQMLKFGEYVHTLFVPTSLNMVRFHPLKGTALITM 116
E FAR S+ +R V + V L + E++ ++ P++L ++ PLKG A++ +
Sbjct: 60 ETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEV 119

Query: 117 EARLVFILVDNFFGGDGRFHAKIEGREFTPTERRIVQLLLKIIFEDYKDAWAPVMDVEFD 176
+ + F ++D FGG G+ R+ T E +++ ++ I + +++W V+D+
Sbjct: 120 DPSITFSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPR 177

Query: 177 YLDSEVNPAMANIVSPTEVVVINSFHIEVDGGGGDFHITMPYSMIEPIRELLDAG--VQS 234
E NP A IV P+E+VV+ + +V G + +PY IEPI L + S
Sbjct: 178 LGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSS 237

Query: 235 DKQDTDMRWSQALHDEIMDVKVGFDASVVEHELTLKDVMNFKAGDIIPIE---LPEYIMM 291
++ + ++ L D++ V + A V L+++D++ + GDII + + + ++
Sbjct: 238 VRRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVL 297

Query: 292 KIEDLPTYRCKMGRSRDNLALKIYEKI 318
I + + C+ G +A +I E+I
Sbjct: 298 SIGNRKKFLCQPGVVGKKIAAQILERI 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1350FLGMOTORFLIN1116e-35 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 111 bits (279), Expect = 6e-35
Identities = 54/122 (44%), Positives = 81/122 (66%)

Query: 2 STDDDWAAAMAEQALEEANAIDLDELVDESQPISKAEAAKLDTILDIPVTISMEVGRSYI 61
+ DD WA A+ EQ + +D I+DIPV +++E+GR+ +
Sbjct: 14 ALDDLWADALNEQKATTTKSAADAVFQQLGGGDVSGAMQDIDLIMDIPVKLTVELGRTRM 73

Query: 62 SIRNLLQLNQGSVVELDRVAGEPLDVMVNGTLIAHGEVVVVNDKFGIRLTDVISQTERIK 121
+I+ LL+L QGSVV LD +AGEPLD+++NG LIA GEVVVV DK+G+R+TD+I+ +ER++
Sbjct: 74 TIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSERMR 133

Query: 122 KL 123
+L
Sbjct: 134 RL 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1352FLGBIOSNFLIP2762e-96 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 276 bits (707), Expect = 2e-96
Identities = 121/240 (50%), Positives = 175/240 (72%)

Query: 8 FIGVSTLLFAASVGAADGVLPAVTVKTAADGSTEYSVTMQILLLMTSLSFIPAMVIMLTS 67
+ V+ +L A LP +T + G +S+ +Q L+ +TSL+FIPA+++M+TS
Sbjct: 4 LLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMMTS 63

Query: 68 FTRIIVVLSILRQAIGLQQTPSNQVLIGMSLFMTFFIMAPVFDKIYDQGVKPYIDEQLTL 127
FTRII+V +LR A+G P NQVL+G++LF+TFFIM+PV DKIY +P+ +E++++
Sbjct: 64 FTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKISM 123

Query: 128 QQAFDKGKEPLRAFMLGQVRTTDLKTFIDISGYQNINSPEEAPMSVLVPAFITSELKTAF 187
Q+A +KG +PLR FML Q R DL F ++ + PE PM +L+PA++TSELKTAF
Sbjct: 124 QEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKTAF 183

Query: 188 QIGFMLFVPFLVLDLVVASILMAMGMMMLSPMIVSLPFKIMLFVLVDGWGLVMGTLANSF 247
QIGF +F+PFL++DLV+AS+LMA+GMMM+ P ++LPFK+MLFVLVDGW L++G+LA SF
Sbjct: 184 QIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1353TYPE3IMQPROT471e-10 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 47.1 bits (112), Expect = 1e-10
Identities = 20/73 (27%), Positives = 39/73 (53%)

Query: 4 EALIDIFREALAVIVMMVSAIVLPGLGIGLIVAVFQAATSINEQTLSFLPRLIVTLLALM 63
+ L+ +AL +++++ + IGL+V +FQ T + EQTL F +L+ L L
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 VMGHWLVQTLMDF 76
++ W + L+ +
Sbjct: 62 LLSGWYGEVLLSY 74


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1354TYPE3IMRPROT1234e-36 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 123 bits (310), Expect = 4e-36
Identities = 94/243 (38%), Positives = 142/243 (58%), Gaps = 1/243 (0%)

Query: 15 YMWPLFRVTSMLMVMVVFGATTTPTRVRLLLAVTITLAIAPVLPPVKDAELFSLSAVFIT 74
Y WPL RV +++ + + P RV+L LA+ IT AIAP LP D +FS A+++
Sbjct: 16 YFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLP-ANDVPVFSFFALWLA 74

Query: 75 AQQIIIGVAMGFVTQMVMQTFVLTGQIIGMQTSLGFASMVDPGSGQQTPVIGNFFLLLAT 134
QQI+IG+A+GF Q G+IIG+Q L FA+ VDP S PV+ +LA
Sbjct: 75 VQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIMDMLAL 134

Query: 135 LIFLAVDGHLLMIRMLVASFETLPISNQGLTLTSYRSLAEWGSYMFGAALTMSLSAIIAL 194
L+FL +GHL +I +LV +F TLPI + L ++ +L + GS +F L ++L I L
Sbjct: 135 LLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLALPLITLL 194

Query: 195 LLVNLSFGVMTRAAPQLNIFSIGFPITMIGGLLILWLTLTPVMAHFEEVWASAQLLLCDI 254
L +NL+ G++ R APQL+IF IGFP+T+ G+ ++ + + E +++ LL DI
Sbjct: 195 LTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADI 254

Query: 255 LGL 257
+
Sbjct: 255 ISE 257


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1355TYPE3IMSPROT337e-117 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 337 bits (866), Expect = e-117
Identities = 98/347 (28%), Positives = 178/347 (51%), Gaps = 2/347 (0%)

Query: 6 SGERSEEPTGRRLEQAREKGQVARSKELGTATVLLSAATGLYMLGPGIAKALSNVFERVF 65
SGE++E+PT +++ AR+KGQVA+SKE+ + ++++ + L L + S + +
Sbjct: 2 SGEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLM--LI 59

Query: 66 TMERAAIFDTNQMFNVWGVVGSEIGWPLLKIMLLIVVVAFIGNVSLGGMNFSTQAMMPKA 125
E++ + + + V V E + ++ + ++A +V G S +A+ P
Sbjct: 60 PAEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDI 119

Query: 126 SKMSPIAGFKRMFGVQALVELTKGIAKFSVVAIAAYLLLSHYFNDILLLSADHLPGNVHH 185
K++PI G KR+F +++LVE K I K +++I ++++ +L L +
Sbjct: 120 KKINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPL 179

Query: 186 ALDLLVWMFILLCSSVLVIVVIDVPFQIWNHNKQLKMTKQEVKDEYKDTEGKPEVKGRVR 245
+L + ++ +VI + D F+ + + K+LKM+K E+K EYK+ EG PE+K + R
Sbjct: 180 LGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRR 239

Query: 246 QMQRELAQRRMMAEVPNADVIVVNPEHYAVAIKYDVKRSAAPFVIAKGVDEVAFKIREVA 305
Q +E+ R M V + V+V NP H A+ I Y + P V K D +R++A
Sbjct: 240 QFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIA 299

Query: 306 RAHNIAIVSAPPLARAIYHTTKLEQQIPEGLFTAVAQVLAYVFQLRQ 352
+ I+ PLARA+Y ++ IP A A+VL ++ +
Sbjct: 300 EEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNI 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1356HTHFIS310.026 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.026
Identities = 25/158 (15%), Positives = 51/158 (32%), Gaps = 19/158 (12%)

Query: 485 VVDAATVVATHISQILTNNAAKLLGYEEVQQLMDMLAKHSPKLVDGFIPDV-MPLGNVVK 543
V D + T ++Q L+ + L +A LV + DV MP N
Sbjct: 8 VADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLV---VTDVVMPDENAFD 64

Query: 544 VMQNLLNEGVSVR--------DLRTIVQTL----LEYGTKSNDTEVLTAAVRIAL---KR 588
++ + + T ++ +Y K D L + AL KR
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 589 MIVQEISGPELEIPVITLAPELEQMLHQSMQATGGDGP 626
+ + +P++ + ++++ + D
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLT 162


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1357PF05272310.013 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.013
Identities = 9/25 (36%), Positives = 12/25 (48%)

Query: 238 VKQGGVVALVGPTGVGKTTSLAKLA 262
K V L G G+GK+T + L
Sbjct: 593 CKFDYSVVLEGTGGIGKSTLINTLV 617


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1360HTHFIS903e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.9 bits (223), Expect = 3e-24
Identities = 33/105 (31%), Positives = 51/105 (48%), Gaps = 3/105 (2%)

Query: 6 KILIVDDFSTMRRIIKNLLRDLGFNNTQEADDGSTALPMLQKGDFDFVVTDWNMPGMQGI 65
IL+ DD + +R ++ L G++ + +T + GD D VVTD MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 DLLKAIRADDSLKHLPVLMVTAEAKREQIIAAAQAGVNGYVVKPF 110
DLL I+ LPVL+++A+ I A++ G Y+ KPF
Sbjct: 64 DLLPRIKKAR--PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1362PF06580464e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 45.6 bits (108), Expect = 4e-07
Identities = 28/182 (15%), Positives = 54/182 (29%), Gaps = 68/182 (37%)

Query: 454 TLNKEIDLVLV---------GEETDLDKNLVEALADPLVH------LVRNSVDHGIEMPN 498
+L E+ +V + + + A+ D V LV N + HGI
Sbjct: 217 SLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGIA--- 273

Query: 499 DREASGKPRTGTITLSASQEGDHILLKIEDDGAGMDPEKLKQIAIKRGVLDEDTAARMTD 558
P+ G I L +++ + L++E+ G+
Sbjct: 274 -----QLPQGGKILLKGTKDNGTVTLEVENTGSLALKN---------------------- 306

Query: 559 SEAYNLIFAPGFSTKVEISDISGRGVGMDVVKTRITQLNG---TVHIDSMKGKGTVLEIK 615
G G+ V+ R+ L G + + +GK + +
Sbjct: 307 -------------------TKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM-VL 346

Query: 616 VP 617
+P
Sbjct: 347 IP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1363HTHFIS651e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.9 bits (158), Expect = 1e-13
Identities = 28/135 (20%), Positives = 56/135 (41%), Gaps = 7/135 (5%)

Query: 2 AIKVLVVDDSSFFRRRVSEIVNQDPELEVIATASNGAEAVKMAAELNPQVITMDIEMPVM 61
+LV DD + R +++ +++ + SN A + A + ++ D+ MP
Sbjct: 3 GATILVADDDAAIRTVLNQALSR--AGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 62 DGITAVREIMAKCP-TPILMFSSLTHDGAKATLDALDAGALDFLPKRF--EDIATNKDEA 118
+ + I P P+L+ S+ + + A + GA D+LPK F ++ A
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSA--QNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 119 ILLLQQRVKALGRRR 133
+ ++R L
Sbjct: 119 LAEPKRRPSKLEDDS 133


71Shewana3_1437Shewana3_1447N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_14370202.337114preprotein translocase subunit SecD
Shewana3_14383162.248453preprotein translocase subunit SecF
Shewana3_14392162.831884hypothetical protein
Shewana3_14402152.575522rhodanese domain-containing protein
Shewana3_14410112.343459siroheme synthase
Shewana3_1442-1122.247911YaeQ family protein
Shewana3_1443-1132.020120peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_1444-1131.880442rhodanese domain-containing protein
Shewana3_1445-1141.127930hypothetical protein
Shewana3_1446-1131.343720acriflavin resistance protein
Shewana3_1447-1150.513145RND family efflux transporter MFP subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1437SECFTRNLCASE802e-18 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 79.9 bits (197), Expect = 2e-18
Identities = 32/165 (19%), Positives = 81/165 (49%), Gaps = 4/165 (2%)

Query: 434 VSIVEERTIGPSLGAENIESGVQAMIWGMAVVLIFMLVYYR-SFGLIANLALTANLVMVV 492
+ I ++GP + E + + V +++ V++ ++ V + F L A +AL ++++ V
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 493 GVMSMIPGAVLTLPGIAGMVLTVGMAVDGNVLIYERIREELRA--GRSVQQAIHEGYGNA 550
G+ +++ L +A ++ G +++ V++++R+RE L ++ ++
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 551 FSTIADANITTFLTALILFAVGTGAIKGFAVTLMIGIATSMFTAI 595
S +TT L + + G I+GF ++ G+ T ++++
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSV 298


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1438SECFTRNLCASE314e-109 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 314 bits (807), Expect = e-109
Identities = 111/309 (35%), Positives = 178/309 (57%), Gaps = 14/309 (4%)

Query: 2 LEILSLKRTVNFLRHALPISIMSAILVFGSLVSLATKGINWGLDFTGGTVVEMEFTQPVD 61
L+++ K +F R + +++ S++ G+N+G+DF GGT + E T +D
Sbjct: 5 LKLVPEKTNFDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAID 64

Query: 62 LNVLRTKLSAPELDGAVVQNFGSSR------DVLVRLSVKE--------GVSSDVQVKSV 107
+ V R L EL ++ ++R+ ++E G V V
Sbjct: 65 VGVYRAALEPLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKV 124

Query: 108 MAAAQQVDAGVQQKRVEFVGPQVGKELAEQGGLAVLVALICIMIYVSFRFEWRLAFGSVA 167
A VD ++ E VGP+V EL ++L A + IM Y+ RFEW+ A G+V
Sbjct: 125 ETALTAVDPALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVV 184

Query: 168 ALAHDVIVTLGVFSVFQLEFDLTVLAGVLTVVGYSLNDTIVVFDRIRENFLKMRKSEPEE 227
AL HDV++T+G+F+V QL+FDLT +A +LT+ GYS+NDT+VVFDR+REN +K + +
Sbjct: 185 ALVHDVLLTVGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRD 244

Query: 228 VVNVSITQTMSRTIITTGTTLVTVVALFLKGGTMIHGFATALLLGIFVGTYSSIYVASYL 287
V+N+S+ +T+SRT++T TTL+ +V + + GG +I GF A++ G+F GTYSS+YVA +
Sbjct: 245 VMNLSVNETLSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNI 304

Query: 288 AIKLGICRE 296
+ +G+ R
Sbjct: 305 VLFIGLDRN 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1443SUBTILISIN1725e-50 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 172 bits (438), Expect = 5e-50
Identities = 70/217 (32%), Positives = 111/217 (51%), Gaps = 10/217 (4%)

Query: 142 TTPWGQTFVGATQLSDSQAG-NRTICIIDSGYDRGHSDLSGNNVTGTN--NSGTGNWYEP 198
P G + A + + G + ++D+G D H DL + G N + G+
Sbjct: 21 EIPRGVEMIQAPAVWNQTRGRGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGDPEIF 80

Query: 199 GNNNAHGTHVAGTIAAIANNDGVIGVMPNQNANIHVIKVFNEAGWGYSSSLVSAVDTCVA 258
+ N HGTHVAGTIAA N +GV+GV P A++ +IKV N+ G G ++ + +
Sbjct: 81 KDYNGHGTHVAGTIAATENENGVVGVAPE--ADLLIIKVLNKQGSGQYDWIIQGIYYAIE 138

Query: 259 NGANVVTMSLGGAGSSTTERNALAAHYNNGVLLIAAAGNDG-----NNTHSYPASYDAVM 313
++++MSLGG A+ + +L++ AAGN+G + YP Y+ V+
Sbjct: 139 QKVDIISMSLGGPEDVPELHEAVKKAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVI 198

Query: 314 SVASVDNHKDHSAFSQYTNQVEISGPGEAILSTVTRG 350
SV +++ + S FS N+V++ PGE ILSTV G
Sbjct: 199 SVGAINFDRHASEFSNSNNEVDLVAPGEDILSTVPGG 235



Score = 67.9 bits (166), Expect = 3e-14
Identities = 28/146 (19%), Positives = 50/146 (34%), Gaps = 16/146 (10%)

Query: 431 NAVKACKNAGASAVIVYSNSALPGLQNPFLVDANSEINMVSV-SVDRATGLALRNQLGAT 489
AVK + + N + L ++SV +++ + +
Sbjct: 159 EAVKKAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVISVGAINFDRHASEFSNSNNE 218

Query: 490 VTVSNQG--------NKDYEYYNGTSMATPHVSGVATLVWS-----YHPECSAAQVRNAL 536
V + G Y ++GTSMATPHV+G L+ + + + ++ L
Sbjct: 219 VDLVAPGEDILSTVPGGKYATFSGTSMATPHVAGALALIKQLANASFERDLTEPELYAQL 278

Query: 537 KQTAEDLGTAGRDDYYGYGLVNAVAA 562
+ LG G GL+ A
Sbjct: 279 IKRTIPLG--NSPKMEGNGLLYLTAV 302


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1444PF05616270.024 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 27.0 bits (59), Expect = 0.024
Identities = 13/37 (35%), Positives = 20/37 (54%)

Query: 52 DVRTPEEFAEGHLANAVNIPFEQVAAEFAKRGIAKDA 88
D+ + E + A +N+P E V EF K GI +D+
Sbjct: 407 DILACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQDS 443


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1446ACRIFLAVINRP487e-157 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 487 bits (1254), Expect = e-157
Identities = 219/1066 (20%), Positives = 427/1066 (40%), Gaps = 79/1066 (7%)

Query: 29 LLALLGLLLGLFAILVTPKEEEPQIDVTFADVFIPFPGATPTEVENLVTLPAEQVISELK 88
+LA++ ++ G AIL P + P I V +PGA V++ VT EQ ++ +
Sbjct: 14 VLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGID 73

Query: 89 GIDTLYSFSQPDG-AMIIVIFKVGVTRNDAIVSLYNQIYSNMDKLPQGAGVGEPLIKPRG 147
+ + S S G I + F+ G + A V + N++ LPQ V + I
Sbjct: 74 NLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE--VQQQGISVEK 131

Query: 148 IDDVPIVSLTLWSKDKQVSAEQLTHLA-LGLETEIKRIPGTREIYTVGQHEMVANVRIDP 206
++ S + + + ++ ++ + R+ G ++ G + + +D
Sbjct: 132 SSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQYAMR-IWLDA 190

Query: 207 AKMNSFNLTYDKLRQSLNDNN------HISMPASLVQGNQEIKVQAGQFLQSIDDVKQLV 260
+N + LT + L N + +L + A ++ ++ ++
Sbjct: 191 DLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNPEEFGKVT 250

Query: 261 VSISQDKQGKPIPVYLADTADISLKSDIPTQSVWHSDKTDIYPAVTIAIGKQPGQNAVDI 320
+ ++ D V L D A + L + + K PA + I G NA+D
Sbjct: 251 LRVNSDGS----VVRLKDVARVELGGENYNVIARINGK----PAAGLGIKLATGANALDT 302

Query: 321 ADATLARIAKVKNVLIPSNVEVTVSRNYGETAADKSNTLILKLIFATSAVVVLVFLTMGA 380
A A A++A+++ P ++V + + ++ L A V ++++L +
Sbjct: 303 AKAIKAKLAELQPFF-PQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 381 -RESLVVGVAIIITLAITLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHMA 439
R +L+ +A+ + L T A+G+++N +++F ++ +IG+LVDDAIVVVEN+ R M
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 440 LGKKSFSELIPVAVDEVGGPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMLIS 499
K E ++ ++ G + + A +PMAF G G I M +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 500 LVVAFMVTPWLSRKLLKHHSGSATNTAHSSDADAQMNESKMVRLFTRLIGPFLLGKGARK 559
++VA ++TP L LLK S V +T +G L
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKIL----GST 537

Query: 560 ARIGLAAGVFVLIGIAVALPVGQLVVLKMLPFDNKSEFQVMVDMPEGTPVEQTQRVLQDL 619
R L + V + + L + LP +++ F M+ +P G E+TQ+VL +
Sbjct: 538 GRYLLIYALIVAGMVVLFLRLPS----SFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 620 SRYLATVPEVEHLQLYAGTNAPMNFNGLVRHYFLRHSQELGDIQVNLVDKKHRKRDSHSI 679
+ Y + ++ T +F+G +Q G V+L + R D +S
Sbjct: 594 TDYYLKNEKANVESVF--TVNGFSFSGQ--------AQNAGMAFVSLKPWEERNGDENSA 643

Query: 680 ALSVREELQHIGAKYQANVKVVEVPPGPPVWSPIVAEVYGPSPAIREQAAYELQSLFRET 739
+ +G V P + A G + +QA +L +
Sbjct: 644 EAVIHRAKMELGKIRDGF---VIPFNMPAIVELGTAT--GFDFELIDQAGLGHDALTQAR 698

Query: 740 KDVVDIDIFLPAA-----------QQKWQVMIDRSKASLMAVPYSNIVDLIATSVGGKDV 788
++ + PA+ ++++ +D+ KA + V S+I I+T++GG V
Sbjct: 699 NQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYV 758

Query: 789 SYLHIAQQKQPVPIRLQLQEGAKIDLEQVLNMKLQSQTGQSVPVSELVTIKRGKIDAPII 848
+ + + + +Q ++ E V + ++S G+ VP S T +
Sbjct: 759 N--DFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLE 816

Query: 849 HKNMIPMVMVVADMAGPLDSPLYGMFDMAGKIDGEGGLGFDQHYIHQPTGLDSVAVLWDG 908
N +P + + G G+ + P G + W G
Sbjct: 817 RYNGLPSMEIQG-------------EAAPGTSSGDAMALMENLASKLPAG---IGYDWTG 860

Query: 909 EWKITYETFRDMGIAYAVGMIAIYLLVVAQFRSYLVPLIIMAPIPLTVIGVMPGHALLGA 968
+ A+ + ++L + A + S+ +P+ +M +PL ++GV+ L
Sbjct: 861 MSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQ 920

Query: 969 QFTATSMIGMIALAGIIVRNSILLVDFINQ-ETASGVPFERAVIHSGAVRAKPIMLTALA 1027
+ M+G++ G+ +N+IL+V+F G A + + +R +PI++T+LA
Sbjct: 921 KNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLA 980

Query: 1028 AMIGALFILDDP-----IFNGLAISLIFGIFISTLLTLIVIPVLYY 1068
++G L + N + I ++ G+ +TLL + +PV +
Sbjct: 981 FILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFV 1026



Score = 75.6 bits (186), Expect = 8e-16
Identities = 83/518 (16%), Positives = 180/518 (34%), Gaps = 46/518 (8%)

Query: 15 GRIAAAFQNSAITPLLALLGLLLGLFAI-LVTPKEEEPQIDVTFADVFIPFPGATPTEVE 73
S LL ++ G+ + L P P+ D I P E
Sbjct: 527 TNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERT 586

Query: 74 NLVTLPAEQVI--SELKGIDTLYSFSQ-------PDGAMIIVIFK---VGVTRNDAIVSL 121
V +E ++++++ + + M V K ++ ++
Sbjct: 587 QKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAV 646

Query: 122 YNQIYSNMDKLPQGAGVGEPLIKPRGIDDVPIVSLTLWSKDKQ-VSAEQLTHLALGLETE 180
++ + K+ G P P ++ D+ + + LT L
Sbjct: 647 IHRAKMELGKIRDG--FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGM 704

Query: 181 IKRIPGTREIYTVGQHEMVA--NVRIDPAKMNSFNLTYDKLRQSLNDNNHISMPASLVQG 238
+ P + E A + +D K + ++ + Q+++ + +
Sbjct: 705 AAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDR 764

Query: 239 NQEIKV---QAGQFLQSIDDVKQLVVSISQDKQGKPIPVYLADTADISLKSDIPTQSVWH 295
+ K+ +F +DV +L V G+ +P + +
Sbjct: 765 GRVKKLYVQADAKFRMLPEDVDKLYVRS---ANGEMVP--FSAFTTSHWVYG-SPRLE-- 816

Query: 296 SDKTDIYPAVTIAIGKQPGQNAVDIADATLARIAKVKNVLIPSNVEVTVSRNYGETAADK 355
+ + P++ I PG DA +A + + + L P+ + + G + ++
Sbjct: 817 --RYNGLPSMEIQGEAAPG---TSSGDA-MALMENLASKL-PAGIGYDWT---GMSYQER 866

Query: 356 SNTLILKLIFATSAVVVLVFLTMGAR-ESLVVGVAIIITLAIT----LFASWAWGFTLNR 410
+ + A S V+VFL + A ES + V++++ + + L A+ + +
Sbjct: 867 LSGNQAPALVAIS--FVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDV 924

Query: 411 VSLFALIFSIGILVDDAIVVVENIHRHMALGKKSFSELIPVAVDEVGGPTILATFTVIAA 470
+ L+ +IG+ +AI++VE M K E +AV P ++ + I
Sbjct: 925 YFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILG 984

Query: 471 LLPMAFVSGLMGPYMSPIPINASMGMLISLVVAFMVTP 508
+LP+A +G + + I GM+ + ++A P
Sbjct: 985 VLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVP 1022



Score = 59.1 bits (143), Expect = 8e-11
Identities = 35/166 (21%), Positives = 76/166 (45%), Gaps = 10/166 (6%)

Query: 915 ETFRDMGIAYAVGMIAIYLLVVAQFRSYLVPLIIMAPIPLTVIGVMPGHALLGAQFTATS 974
E + + A + + +YL + R+ L+P I +P+ ++G A G +
Sbjct: 339 EVVKTLFEAIMLVFLVMYL-FLQNMRATLIPTIA---VPVVLLGTFAILAAFGYSINTLT 394

Query: 975 MIGMIALAGIIVRNSILLVDFINQETAS-GVPFERAVIHS-----GAVRAKPIMLTALAA 1028
M GM+ G++V ++I++V+ + + +P + A S GA+ ++L+A+
Sbjct: 395 MFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFI 454

Query: 1029 MIGALFILDDPIFNGLAISLIFGIFISTLLTLIVIPVLYYAAMKNR 1074
+ I+ +I+++ + +S L+ LI+ P L +K
Sbjct: 455 PMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPV 500


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1447RTXTOXIND408e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 40.2 bits (94), Expect = 8e-06
Identities = 38/259 (14%), Positives = 76/259 (29%), Gaps = 66/259 (25%)

Query: 75 AALLEITSKEQGAELASYEADLAKATALNVEAQAQYKRYKELFPQGAISK---------- 124
E+ ++ AE + A + + L+ +++ + L + AI+K
Sbjct: 202 KYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKY 261

Query: 125 ----GAMDEATANAKAAEQAVSAAKARVI-----------------------------KA 151
+ + + E + +AK K
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKN 321

Query: 152 TESLKYTVVSAPFSGIVTERLV-ELGETVSVGQPLLSGFSPSQ--MRAITQVPQRYIQQL 208
E + +V+ AP S V + V G V+ + L+ P + V + I +
Sbjct: 322 EERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMV-IVPEDDTLEVTALVQNKDIGFI 380

Query: 209 KNAPEFMVRLS--DGRE---LTSKDLTIFSFADPVSH-----SYQVRINLPKDEP----- 253
++++ L K I D + + V I++ ++
Sbjct: 381 NVGQNAIIKVEAFPYTRYGYLVGKVKNI--NLDAIEDQRLGLVFNVIISIEENCLSTGNK 438

Query: 254 --NLQPGTWAKALFKNGER 270
L G A K G R
Sbjct: 439 NIPLSSGMAVTAEIKTGMR 457



Score = 30.6 bits (69), Expect = 0.009
Identities = 18/75 (24%), Positives = 26/75 (34%), Gaps = 1/75 (1%)

Query: 159 VVSAPFSGIVTERLVELGETVSVGQPLLSGFSPSQMRAITQVPQRYIQQLKNAPEFMVRL 218
+ + IV E +V+ GE+V G LL + A T Q + Q + L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLK-LTALGAEADTLKTQSSLLQARLEQTRYQIL 156

Query: 219 SDGRELTSKDLTIFS 233
S EL
Sbjct: 157 SRSIELNKLPELKLP 171


72Shewana3_1566Shewana3_1569N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_15660101.148609putative sulfate transport protein CysZ
Shewana3_15671111.398987chromosome segregation protein SMC
Shewana3_1568-1141.141044cell division protein ZipA
Shewana3_15690150.834283NAD-dependent DNA ligase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1566PF06580280.042 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 27.9 bits (62), Expect = 0.042
Identities = 15/103 (14%), Positives = 35/103 (33%), Gaps = 7/103 (6%)

Query: 21 GFGLIKRKGLRTFVFIPLMINLVLFAAVIYVAIGQLDVLFTWMNAQLPEYLSWLNF---- 76
G+G+ L F F L + L + + +AI + ++ T + WL
Sbjct: 19 GWGVY---TLTGFGFASLYGSPKLHSMIFNIAISLMGLVLTHAYRSFIKRQGWLKLNMGQ 75

Query: 77 LLWPLAVTTMLVMLAFVFSSVMNWLAAPFNGLLAEKVEQLLTG 119
++ + +++ + + ++ W F L
Sbjct: 76 IILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPLAL 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1567GPOSANCHOR475e-07 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 46.6 bits (110), Expect = 5e-07
Identities = 42/293 (14%), Positives = 104/293 (35%), Gaps = 9/293 (3%)

Query: 616 AKQDNSQSLVQLSKEQTQLSEAIAECEQAKAIQQAKLDELAQQLTQVRDSLSQGTKRLHQ 675
A + + +L ++ + + + + L ++ + LS ++L +
Sbjct: 44 ATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRK 103

Query: 676 LQLDKATKSTQLNNAEAQAKQREAKRGQLAETVARTQAELAELAEQLMLLAEQEDELAEA 735
+ K++++ EA+ E A++ L + LA ++ +L +A
Sbjct: 104 NDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKA 163

Query: 736 LEVSLEQQQQQSQDAQGDMARHQALKAQIGDAERRLASLNASLQSVTTRMAVSTEQIELQ 795
LE ++ S + A AL+A+ + E+ L + + ++ +
Sbjct: 164 LEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAAL 223

Query: 796 RVRVSELVHSKETLSA--QLANVAAQEGDQQTVQLSEQLAQLLNQQQGQQQALKSLRSQQ 853
R ++L + E + + + + L + A+L +G + ++
Sbjct: 224 AARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKI 283

Query: 854 SSLTETLNSIGLKQKQELGKLEGLTQSLSTLKLRREGLKGQADSQLAALSEQQ 906
+L + E LE +Q L+ R+ L+ D+ A + +
Sbjct: 284 KTLEAEKA----ALEAEKADLEHQSQVLNA---NRQSLRRDLDASREAKKQLE 329



Score = 43.1 bits (101), Expect = 6e-06
Identities = 30/182 (16%), Positives = 69/182 (37%), Gaps = 7/182 (3%)

Query: 188 RENLERLGDIRSELAKQLEKLSQQAKAAKQYRELKQAERKTHAELLVMRYQELQSQMASL 247
+ +LE+ + + + +A K E +QAE + E + +++ +L
Sbjct: 157 KADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTL 216

Query: 248 SEQISSLELQQAAAQSLAQTGELESTELQLKLSQLAEQEQQAVEAYYLTGTEIAKLEQQL 307
+ ++L ++A + + ST K+ L ++ A+LE+ L
Sbjct: 217 EAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEA-------RQAELEKAL 269

Query: 308 QSQKQRDAQLHNQLEQLSEQIIQNQAKLAAYQASFQALEAELSQLAPQHELQQEMMDELQ 367
+ +++ L + +A+ A + Q L A L + +E +L+
Sbjct: 270 EGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLE 329

Query: 368 AQ 369
A+
Sbjct: 330 AE 331



Score = 42.7 bits (100), Expect = 8e-06
Identities = 58/361 (16%), Positives = 131/361 (36%), Gaps = 30/361 (8%)

Query: 146 QGTISRLIESKPQDLRTFIEEAAGISRYKERRRETENRIRHTRENLERLGDIRSELAKQL 205
+S E ++ ++ E+A+ I + R+ + E + L +
Sbjct: 91 TEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEK 150

Query: 206 EKLSQQAKAAKQYRELKQAERKTHAELLVMRYQELQSQMASLSEQISSLELQQAAAQSLA 265
L+ + ++ E + + + L+++ A+L + + LE A + +
Sbjct: 151 AALAARKADLEKALEGAMNFSTADSA----KIKTLEAEKAALEARQAELEKALEGAMNFS 206

Query: 266 QTGELESTELQLKLSQLAEQEQQAVEAYYLTGTEIAKLEQQLQSQKQRDAQLHNQLEQLS 325
+ L+ + + LA ++ +A ++++ + A L + +L
Sbjct: 207 TADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELE 266

Query: 326 EQIIQNQAKLAAYQASFQALEAELSQLAPQHELQQEMMDELQAQWEMSVSRSEAQSESAR 385
+ + A A + LEAE + L + ++ + Q A +S R
Sbjct: 267 KALEGAMNFSTADSAKIKTLEAEKAALE---AEKADLEHQSQV--------LNANRQSLR 315

Query: 386 VLAAAVAQHKLQLELHRSKLAHQQQLNAHKTQLHQEQQQELASLNAHALEDNSASLNDEI 445
A + K QLE KL Q +++ Q +L + + +
Sbjct: 316 RDLDASREAKKQLEAEHQKLEEQNKISEASRQ---------------SLRRDLDASREAK 360

Query: 446 TQLEQALAEQVEINQGFESTLAAVTHTLDVARGEFEQLSQRLTSMRARFELVEQWLAKQE 505
QLE + E N+ E++ ++ LD +R +Q+ + L ++ +E+ + E
Sbjct: 361 KQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELE 420

Query: 506 E 506
E
Sbjct: 421 E 421



Score = 42.0 bits (98), Expect = 1e-05
Identities = 47/282 (16%), Positives = 93/282 (32%), Gaps = 11/282 (3%)

Query: 231 ELLVMRYQELQSQMASLSEQISSLELQQAAAQSLAQTGELESTELQLK---LSQLAEQEQ 287
L ++ +L +L + L + + A+ + + +E K L +
Sbjct: 67 NTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLE 126

Query: 288 QAVEAYYLTGTEIAKLEQQLQSQKQRDAQLHNQLEQLSEQIIQNQAKLAAYQASFQALEA 347
+A+E T + + L+++K A LE+ E A A + LEA
Sbjct: 127 KALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA---MNFSTADSAKIKTLEA 183

Query: 348 ELSQLAPQHELQQEMMDELQAQWEMSVSRSEAQSESARVLAAAVAQHKLQLELHRSKLAH 407
E + L + ++ ++ ++ + LAA A + LE +
Sbjct: 184 EKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTA 243

Query: 408 QQQLNAHKTQLHQEQQQELASLNAHALEDNSASLNDEITQLEQALAEQVEINQGFESTLA 467
+ A L ALE + +++ AE+ + A
Sbjct: 244 DSAKIKTLEAEKAALEARQAELEK-ALEGAMNFSTADSAKIKTLEAEKAALEA----EKA 298

Query: 468 AVTHTLDVARGEFEQLSQRLTSMRARFELVEQWLAKQEELSD 509
+ H V + L + L + R + +E K EE +
Sbjct: 299 DLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNK 340



Score = 34.3 bits (78), Expect = 0.003
Identities = 58/390 (14%), Positives = 124/390 (31%), Gaps = 28/390 (7%)

Query: 430 NAHALEDNSASLNDEITQLEQALAEQVEINQGFESTLAAVTHTLDVARGEFEQLSQRLTS 489
+++ + E L+ ++ N+ + +T L A+ + + + L+
Sbjct: 51 TLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSE 110

Query: 490 MRARFELVEQWLA------------KQEELSDKPQLWQSIQVENGWEAAAELALQGLMTL 537
++ + +E A + + L +A E AL+G M
Sbjct: 111 KASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNF 170

Query: 538 PVGVNANEIGFYADAALSADVHLDGSPILDAKLNLAPWLKGLKWADNLASAQAQLPSLAA 597
+A A+ A + L+ +N + + + + +A+ +LAA
Sbjct: 171 STADSAKIKTLEAEKAALEARQAELEKALEGAMNFS-----TADSAKIKTLEAEKAALAA 225

Query: 598 DERIVTADGYLLGKGFLIAKQDNSQSLVQLSKEQTQLSEAIAECEQAKAIQQAKLDELAQ 657
+ + A + S + L A E +A + L+
Sbjct: 226 RKADLEK-----------ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMN 274

Query: 658 QLTQVRDSLSQGTKRLHQLQLDKATKSTQLNNAEAQAKQREAKRGQLAETVARTQAELAE 717
T + L+ +KA Q A + E + +AE +
Sbjct: 275 FSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQK 334

Query: 718 LAEQLMLLAEQEDELAEALEVSLEQQQQQSQDAQGDMARHQALKAQIGDAERRLASLNAS 777
L EQ + L L+ S E ++Q + Q +++ +A R L + +
Sbjct: 335 LEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREA 394

Query: 778 LQSVTTRMAVSTEQIELQRVRVSELVHSKE 807
+ V + + ++ EL SK+
Sbjct: 395 KKQVEKALEEANSKLAALEKLNKELEESKK 424


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1568IGASERPTASE320.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.0 bits (72), Expect = 0.004
Identities = 28/136 (20%), Positives = 42/136 (30%), Gaps = 21/136 (15%)

Query: 70 AVRVRKANEAHTPEAPAFNPYLKQEAKAQPQPVEPVQVEPKPLFKQEPSMAQPDFSLQSP 129
+V A EAP P ++ E + E K + K E Q
Sbjct: 1009 SVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNE----------QDA 1058

Query: 130 TAKEQHRGPKASRQEPVLPGHSANLAQAHVGQSHAAMVAQKAAEEQRAQVQMPTQTALFD 189
T A + + AN V AQ +E + Q +TA +
Sbjct: 1059 TETTAQNREVAKEAKSNVK---ANTQTNEV--------AQSGSETKETQTTETKETATVE 1107

Query: 190 EEEAYEEEQPQVVEQP 205
+EE + E + E P
Sbjct: 1108 KEEKAKVETEKTQEVP 1123



Score = 29.6 bits (66), Expect = 0.020
Identities = 25/140 (17%), Positives = 46/140 (32%), Gaps = 13/140 (9%)

Query: 70 AVRVRKANEAHTPEAPAFNPYLKQEAKAQPQPVEPVQVEPKPLFKQEPSMAQPDFSLQSP 129
+ A E T + K KA Q E Q E Q + ++
Sbjct: 1052 EKNEQDATET-TAQNREVAKEAKSNVKANTQTNEVAQS------GSETKETQTTETKETA 1104

Query: 130 TAKEQHRGPKASRQEPVLPGHSANLA----QAHVGQSHAAMVAQKAAEEQRAQVQMPTQT 185
T +++ + + + +P ++ ++ Q+ Q A + + Q T T
Sbjct: 1105 TVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNT 1164

Query: 186 ALFDEEEAYEEEQPQVVEQP 205
+ E +E VEQP
Sbjct: 1165 T--ADTEQPAKETSSNVEQP 1182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1569HTHTETR320.009 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 31.5 bits (71), Expect = 0.009
Identities = 15/107 (14%), Positives = 37/107 (34%), Gaps = 7/107 (6%)

Query: 504 MLDRMGIKSATNLALAIEAAKTTTLPRFLYALGIREVGETTAANLAT---HFGSLEALRV 560
M + ++ ++ A + + + + E+ + HF L
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 561 ATIEQLIQVEDIGEVVAQHVAHFFAQPHNL--EVIDALIAAGVNWPA 605
E +IGE+ ++ A F P ++ E++ ++ + V
Sbjct: 61 EIWEL--SESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEER 105


73Shewana3_1613Shewana3_1620N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1613538-10.971542rRNA (guanine-N(1)-)-methyltransferase
Shewana3_1615327-7.994305histone family protein DNA-binding protein
Shewana3_1616326-7.458517hypothetical protein
Shewana3_1617222-5.702257hypothetical protein
Shewana3_1618117-4.517934ECF subfamily RNA polymerase sigma-24 factor
Shewana3_1619013-3.430387serine/threonine protein kinase
Shewana3_1620-111-1.157064ABC transporter-like protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1613SUBTILISIN1191e-31 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 119 bits (299), Expect = 1e-31
Identities = 60/247 (24%), Positives = 102/247 (41%), Gaps = 50/247 (20%)

Query: 251 FVYNEQTKLNDPTEYKICINGHGLSVASSIAAIQNNGKGIASAVGSENIDIVPVKVIDSC 310
F +++ +Y NGHG VA +IAA +N + A ++ ++ +KV++
Sbjct: 69 FTDDDEGDPEIFKDY----NGHGTHVAGTIAATENENGVVGVAPEAD---LLIIKVLNKQ 121

Query: 311 TGSALTSDLIKAIYWAAKSDDTFEGLEPISEPVDVINLSLGSNKNELCEVGYNAFADAVD 370
GS +I+ IY+A + VD+I++SLG + +AV
Sbjct: 122 -GSGQYDWIIQGIYYAIEQK------------VDIISMSLGGPE------DVPELHEAVK 162

Query: 371 YAKQKGIVVVAALGNDGVSGD----IFTPATCNGVIPVSSNNVHGQLSYFSSYLSDKRTL 426
A I+V+ A GN+G D + P N VI V + N S FS+ ++ L
Sbjct: 163 KAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVISVGAINFDRHASEFSNSNNEV-DL 221

Query: 427 STIGEDMTLPKVTTTTYIDRNFIDTNCQGSIESCYATGQGTSYSAPIVSGLVSMVLMQNP 486
GED+ +T YAT GTS + P V+G ++++
Sbjct: 222 VAPGEDI------LSTVPG-------------GKYATFSGTSMATPHVAGALALIKQLAN 262

Query: 487 SLNPDEI 493
+ ++
Sbjct: 263 ASFERDL 269


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1615DNABINDINGHU922e-28 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 92.1 bits (229), Expect = 2e-28
Identities = 35/88 (39%), Positives = 52/88 (59%)

Query: 2 NKAQLIQRIATSLEQSQASTRPVVEQILQQIHIALSEGEKVFLPQFGTFELRYHLPKSGR 61
NK LI ++A + E ++ + V+ + + L++GEKV L FG FE+R + GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGETMEIAGFNQPSFKAATALKQAI 89
NPQTGE ++I P+FKA ALK A+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1619YERSSTKINASE340.005 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 33.6 bits (76), Expect = 0.005
Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 1/50 (2%)

Query: 182 QVLDGIIHSHANQVLHRDIKPDNILVDD-DGRVHVIDFGISKLMGEQGNG 230
++LD H V+H DIKP N++ D G VID G+ GEQ G
Sbjct: 253 RLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSGEQPKG 302


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1620PF05272300.019 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.019
Identities = 9/21 (42%), Positives = 12/21 (57%)

Query: 31 PIALVGPNGAGKTTLFSLLCG 51
+ L G G GK+TL + L G
Sbjct: 598 SVVLEGTGGIGKSTLINTLVG 618


74Shewana3_1881Shewana3_1885N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1881-2190.768519redoxin domain-containing protein
Shewana3_1882-2120.831507putative lipoprotein
Shewana3_1883-2111.128220hypothetical protein
Shewana3_1884-2111.047910ApbE family lipoprotein
Shewana3_1885-3120.783380two component transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1881ADHESNFAMILY270.048 Adhesin family signature.
		>ADHESNFAMILY#Adhesin family signature.

Length = 309

Score = 26.7 bits (59), Expect = 0.048
Identities = 8/27 (29%), Positives = 13/27 (48%)

Query: 134 ELVKSHVGFYQDNAADYEQELISLLKE 160
++ FY+ N +Y +L L KE
Sbjct: 160 AKDPNNKEFYEKNLKEYTDKLDKLDKE 186


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1882VACJLIPOPROT280.004 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 27.6 bits (61), Expect = 0.004
Identities = 15/52 (28%), Positives = 21/52 (40%), Gaps = 8/52 (15%)

Query: 3 KIALIAVSLMIVGLTGCSSLGVQ------PWEKGQFARADMALDSEKLDQAL 48
K+ L A++L L GC+S G P E F R + LD +
Sbjct: 2 KLRLSALALGTTLLVGCASSGTDQQGRSDPLEG--FNRTMYNFNFNVLDPYI 51


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1883PF00577310.009 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 31.4 bits (71), Expect = 0.009
Identities = 17/90 (18%), Positives = 34/90 (37%), Gaps = 1/90 (1%)

Query: 88 DLKLVVDSLTGASASGAVAQSDSQTFTRPSGNGQYKVAAGETPLDDTFHDTRVQGSANWS 147
DL++ + G++ V S R G+ +Y + AGE + + +
Sbjct: 343 DLQVTIKEADGSTQIFTVPYSSVPLLQRE-GHTRYSITAGEYRSGNAQQEKPRFFQSTLL 401

Query: 148 QALNSDWKVNGGVYGSKEFDYMSMGINAGL 177
L + W + GG + + + GI +
Sbjct: 402 HGLPAGWTIYGGTQLADRYRAFNFGIGKNM 431


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1885HTHFIS874e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 87.2 bits (216), Expect = 4e-22
Identities = 33/119 (27%), Positives = 58/119 (48%), Gaps = 1/119 (0%)

Query: 2 RIMLVEDNELLAQGICLSLAKMGMQVDHLSSYQQALVGIKNETFSAIVLDLGLPDGNGKE 61
I++ +D+ + + +L++ G V S+ I +V D+ +PD N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 LLKAWRAEGVSIPTIVLTANTDFDTKLECLDIGADDYLGKPFDVRELAARI-RAIVRRQ 119
LL + +P +V++A F T ++ + GA DYL KPFD+ EL I RA+ +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123


75Shewana3_1989Shewana3_1996N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_1989-1151.265699multidrug resistance protein D
Shewana3_1990-1100.797863beta-lactamase
Shewana3_1991-1110.380459DTW domain-containing protein
Shewana3_1992012-0.280579secretion protein HlyD family protein
Shewana3_1993013-0.729844acriflavin resistance protein
Shewana3_1994-116-2.455777outer membrane efflux protein
Shewana3_1995-118-3.226664hypothetical protein
Shewana3_1996-121-5.054636phage integrase family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1989TCRTETB669e-14 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 65.7 bits (160), Expect = 9e-14
Identities = 38/146 (26%), Positives = 64/146 (43%), Gaps = 7/146 (4%)

Query: 24 QLLFMLVFMVACGQMAQTIFVPALPLIAQGLAVDASKLQAVMACYLLAYGLCQFIYGPLS 83
Q+L L + + + + +LP IA + V ++L + + +YG LS
Sbjct: 14 QILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLS 73

Query: 84 DRVGRKMPLLIGIGIFILGALMAA-EATSFNQLIFASLLQGLGTA-----SAGALCRSIP 137
D++G K LL GI I G+++ + F+ LI A +QG G A + R IP
Sbjct: 74 DQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIP 133

Query: 138 RDHYFGDNLVRFNSYVSMAVVFLPLV 163
+++ G S V+M P +
Sbjct: 134 KENR-GKAFGLIGSIVAMGEGVGPAI 158


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1992RTXTOXIND463e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 45.6 bits (108), Expect = 3e-07
Identities = 29/147 (19%), Positives = 63/147 (42%), Gaps = 10/147 (6%)

Query: 111 VNRLKAKLSSQQAILDKAERDVKRLKPLYEQDAASQLDYDNALSTLAQARSNLTASRAEV 170
+L ++ L++ E ++ K Y+ +QL + L L Q N+ E+
Sbjct: 261 YVEAVNELRVYKSQLEQIESEILSAKEEYQL--VTQLFKNEILDKLRQTTDNIGLLTLEL 318

Query: 171 EEAELELSYTEIKAPIAGLVSRSEV-DIGALVGSKGQSLLTRVKQVDPIYVSFNMSALDY 229
+ E + I+AP++ V + +V G +V + ++L+ V + D + V+ + D
Sbjct: 319 AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTT-AETLMVIVPEDDTLEVTALVQNKDI 377

Query: 230 ------LNAQRRLTSYSAKKEAEVEGK 250
NA ++ ++ + + GK
Sbjct: 378 GFINVGQNAIIKVEAFPYTRYGYLVGK 404



Score = 37.9 bits (88), Expect = 6e-05
Identities = 21/176 (11%), Positives = 60/176 (34%), Gaps = 14/176 (7%)

Query: 73 EVRARVDGFVEEKRFVEGSAVKAGELLYQIDNKPYVAVVNRLKAKLSSQ-------QAIL 125
E++ + V+E EG +V+ G++L ++ A + ++ L Q +
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 126 DKAERDVKRLKPLYEQDAASQLDYDNALSTLAQARSNLTASRAEVEEAELELSYTEIKAP 185
E + L ++ + + L + + + + + + EL+ + +A
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQK--YQKELNLDKKRAE 215

Query: 186 IAGLVSRSEVDIGALVGSKGQSLLTRVKQVDPIYVSFNMSALDYLNAQRRLTSYSA 241
+++R I + +R+ + ++ L + +
Sbjct: 216 RLTVLAR----INRYENLS-RVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVN 266


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1993ACRIFLAVINRP9640.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 964 bits (2494), Expect = 0.0
Identities = 425/1028 (41%), Positives = 618/1028 (60%), Gaps = 9/1028 (0%)

Query: 1 MAQFFINRPIFASVISIVIVLLGVIAMFKLPVDQYPYITPPQVTISASYPGASSTTAAES 60
MA FFI RPIFA V++I++++ G +A+ +LPV QYP I PP V++SA+YPGA + T ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VATPLEQEVNGVPNMIYMSSKSTNSGGTSVTITFDVGTNADLAAVDVQNSAQQASGGLPI 120
V +EQ +NG+ N++YMSS S ++G ++T+TF GT+ D+A V VQN Q A+ LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 DVQTEGVTVSKDASVELLKLALTSNDERFDEIYLSNYATINIESALRRIPGVGRTRNTGS 180
+VQ +G++V K +S L+ S++ + +S+Y N++ L R+ GVG + G+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 RSYAMRIWLKPDAMAGYSLTTTDVINAIKAQNKESPAGTIGTQPNNDDISLTLPISVAGR 240
+ YAMRIWL D + Y LT DVIN +K QN + AG +G P L I R
Sbjct: 181 Q-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 241 LSSVQAFNEIIVRANPDGSIIRLRDIAGVELGSSAYTLQSQLNGENATILQVYLLPGANA 300
+ + F ++ +R N DGS++RL+D+A VELG Y + +++NG+ A L + L GANA
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 301 LEVTRKVKQTMAELSQKFPQGMKWEVFYDASIFIQESIDEVIHTLIEALVLVVLVVYLFL 360
L+ + +K +AEL FPQGMK YD + F+Q SI EV+ TL EA++LV LV+YLFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 361 QNVRATLIPAIAVPVSLIGTLAAMLAFGFTINTVSLLALVLAIGIVVDDAIVVVENVERL 420
QN+RATLIP IAVPV L+GT A + AFG++INT+++ +VLAIG++VDDAIVVVENVER+
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 421 IHEKGMSAIDATRIAMKELSGALVATSLVLCAVFVPVSFLAGITGIMYREFAVAITVAVL 480
+ E + +AT +M ++ GALV ++VL AVF+P++F G TG +YR+F++ I A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 481 ISTLVALTLSPALCALLLKPSKAPE----RGFFHWLNRKLDVGTNQYVGLVALTNKYAKR 536
+S LVAL L+PALCA LLKP A GFF W N D N Y V R
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 537 SYLAFAIMFGGTYFIMSHLPSSFMPDEDQGRFFIDMTLPDGSTVNRTEAILKKAEQYVRA 596
L +A++ G + LPSSF+P+EDQG F + LP G+T RT+ +L + Y
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 597 NPAV-AYSFTLAGENRRSGANQANGQFEVVLKPWAEREASHATVQSVMKAIDKDLKNVLE 655
N S SG Q G V LKPW ER + ++V+ +L + +
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 656 AEFNLYLPSAVPGLGNGSGVEMQLQDTSGTHFDGLIETANELVEQLKLQP-EVASASVSL 714
+ A+ LG +G + +L D +G D L + N+L+ P + S +
Sbjct: 660 GFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNG 719

Query: 715 QSAIPQLHLTVDEAKAMAIGVNVSDIYSTIKTLTDSSTVNDFNLFGRVYRVKIQAEESYR 774
Q L VD+ KA A+GV++SDI TI T + VNDF GRV ++ +QA+ +R
Sbjct: 720 LEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFR 779

Query: 775 QFPHQIKDYYVRSSSGAMVPIGVLAKYDYTVGPSSVTHYNLFSSASINVTPATGYATGDV 834
P + YVRS++G MVP + G + YN S I A G ++GD
Sbjct: 780 MLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDA 839

Query: 835 IQAIERVATPILPDEFKYEWTGITYQEVQSANQTGIAIGLALLFVFLFLAALYESWSIPV 894
+ +E +A+ LP Y+WTG++YQE S NQ + ++ + VFL LAALYESWSIPV
Sbjct: 840 MALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPV 898

Query: 895 AVLLIAPIALLGAAVTTLISGMQSNLFFQVAFIALIGMAAKNAILIVEFANQLH-QQGRT 953
+V+L+ P+ ++G + + +++++F V + IG++AKNAILIVEFA L ++G+
Sbjct: 899 SVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKG 958

Query: 954 RISAALEAATMRFRPILMTSMAFILGVLPLVLSEGPGAVSRQSISLPILGGMVLATTIGI 1013
+ A L A MR RPILMTS+AFILGVLPL +S G G+ ++ ++ + ++GGMV AT + I
Sbjct: 959 VVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAI 1018

Query: 1014 VFVPLFFV 1021
FVP+FFV
Sbjct: 1019 FFVPVFFV 1026



Score = 108 bits (272), Expect = 4e-26
Identities = 74/513 (14%), Positives = 183/513 (35%), Gaps = 37/513 (7%)

Query: 5 FINRPIFASVISIVIVLLGVIAMFKLPVDQYPYITPPQVTISASYPGASSTTAAESVATP 64
+ +I +IV V+ +LP P P ++ + V
Sbjct: 533 ILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQ 592

Query: 65 LEQ-----EVNGVPNMIYMSSKSTNSGGTSVTITF------DVGTNADLAAVDVQNSAQQ 113
+ E V ++ ++ S + + + F + + +A V + A+
Sbjct: 593 VTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKM 652

Query: 114 ASGGLP--------IDVQTEGVTVSKDASVELLKLALTSNDERFDEIYLSNYATINIESA 165
G + + E T + EL+ A +D L+ + A
Sbjct: 653 ELGKIRDGFVIPFNMPAIVELGTATG-FDFELIDQAGLGHDA------LTQARNQLLGMA 705

Query: 166 LRRIPGVGRTRNTGS-RSYAMRIWLKPDAMAGYSLTTTDVINAIKAQNKESPAGTIGTQP 224
+ + R G + ++ + + ++ +D+ I + +
Sbjct: 706 AQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRG 765

Query: 225 NNDDISLTLPISVAGRLSSVQAFNEIIVRANPDGSIIRLRDIAGVELGSSAYTLQSQLNG 284
+ + A + +++ VR + +G ++ + L + NG
Sbjct: 766 RVKKLYVQAD---AKFRMLPEDVDKLYVR-SANGEMVPFSAFTTSHWVYGSPRL-ERYNG 820

Query: 285 ENATILQVYLLPGANALEVTRKVKQTMAELSQKFPQGMKWEVFYDASIFIQESIDEVIHT 344
+ +Q PG ++ + ++ ++L P G+ + S + S ++
Sbjct: 821 LPSMEIQGEAAPGTSSGDAMALMENLASKL----PAGI-GYDWTGMSYQERLSGNQAPAL 875

Query: 345 LIEALVLVVLVVYLFLQNVRATLIPAIAVPVSLIGTLAAMLAFGFTINTVSLLALVLAIG 404
+ + V+V L + ++ + + VP+ ++G L A F + ++ L+ IG
Sbjct: 876 VAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIG 935

Query: 405 IVVDDAIVVVENVERLIHEKGMSAIDATRIAMKELSGALVATSLVLCAVFVPVSFLAGIT 464
+ +AI++VE + L+ ++G ++AT +A++ ++ TSL +P++ G
Sbjct: 936 LSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAG 995

Query: 465 GIMYREFAVAITVAVLISTLVALTLSPALCALL 497
+ + ++ +TL+A+ P ++
Sbjct: 996 SGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVI 1028



Score = 74.1 bits (182), Expect = 2e-15
Identities = 79/501 (15%), Positives = 172/501 (34%), Gaps = 37/501 (7%)

Query: 539 LAFAIMFGGTYFIMSHLPSSFMPDEDQGRFFIDMTLPDGSTVNRTEAILKKAEQYVRANP 598
LA +M G I+ LP + P + P + + + EQ +
Sbjct: 15 LAIILMMAGALAILQ-LPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGID 73

Query: 599 AVAYSFTLAGENRRSGANQANGQFEVVLKP-WAEREASHATVQSVMKAIDKDLKNVLEAE 657
+ Y ++ + +G+ F+ P A+ + +Q + ++++
Sbjct: 74 NLMY---MSSTSDSAGSVTITLTFQSGTDPDIAQVQVQ-NKLQLATPLLPQEVQ------ 123

Query: 658 FNLYLPSAVPGLGNGSGVEMQLQDTSGTHFDGLIETANELVEQLKLQPEVAS--ASVSLQ 715
+ + S M S + ++ + +K + V L
Sbjct: 124 -----QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLF 178

Query: 716 SAIPQLHLTVDEAKAMAIGVNVSDIYSTIKTLTDSSTVNDFNLFGRVYRVKIQA---EES 772
A + + +D + D+ + +K D + ++ A ++
Sbjct: 179 GAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQT 238

Query: 773 YRQFPHQIKDYYVRSS-SGAMVPIGVLAK-YDYTVGPSSVTHYNLFSSASINVTPATGYA 830
+ P + +R + G++V + +A+ + + N +A + + ATG
Sbjct: 239 RFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGAN 298

Query: 831 TGDVIQAI-ERVAT--PILPDEFKYEWTGITYQEVQSANQT-----GIAIGLALLFVFLF 882
D +AI ++A P P K + T VQ + AI L L ++LF
Sbjct: 299 ALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLF 358

Query: 883 LAALYESWSIPVAVLLIAPIALLGAAVTTLISGMQSNLFFQVAFIALIGMAAKNAILIVE 942
L ++ + + P+ LLG G N + IG+ +AI++VE
Sbjct: 359 L----QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVE 414

Query: 943 FANQLHQQGRTRISAALEAATMRFR-PILMTSMAFILGVLPLVLSEGPGAVSRQSISLPI 1001
++ + + A E + + + ++ +M +P+ G + S+ I
Sbjct: 415 NVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITI 474

Query: 1002 LGGMVLATTIGIVFVPLFFVT 1022
+ M L+ + ++ P T
Sbjct: 475 VSAMALSVLVALILTPALCAT 495


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_1996SURFACELAYER320.003 Lactobacillus surface layer protein signature.
		>SURFACELAYER#Lactobacillus surface layer protein signature.

Length = 439

Score = 32.3 bits (73), Expect = 0.003
Identities = 14/55 (25%), Positives = 27/55 (49%), Gaps = 1/55 (1%)

Query: 246 SEPFAAYNAVKDWLNESKITEGHLFRSISRDGKTLRPYQISDNVT-SKSSLIRNS 299
++ YN V +N +K+ G + + +GK Y +DN+ +K +L N+
Sbjct: 331 TDKVTRYNTVTVAMNTTKLANGISYYEVIENGKATGKYINADNIDGTKRTLKHNA 385


76Shewana3_2212Shewana3_2227N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2212217-0.235910response regulator receiver modulated
Shewana3_22133170.213174response regulator receiver modulated CheB
Shewana3_22143170.125039chemoreceptor glutamine deamidase CheD
Shewana3_2215215-1.626060chemotaxis protein CheR
Shewana3_2216214-2.042755methyl-accepting chemotaxis sensory transducer
Shewana3_2217014-3.366171CheW protein
Shewana3_2218114-1.195805CheA signal transduction histidine kinase
Shewana3_2219115-1.043437response regulator receiver protein
Shewana3_2220115-0.674037response regulator receiver protein
Shewana3_22211161.262344anti-sigma-factor antagonist
Shewana3_22221171.246634methyl-accepting chemotaxis sensory transducer
Shewana3_22231181.101499CoA-binding domain-containing protein
Shewana3_2224015-0.192145EmrB/QacA family drug resistance transporter
Shewana3_2225-114-0.350157secretion protein HlyD family protein
Shewana3_2226016-0.670370MarR family transcriptional regulator
Shewana3_2227015-0.588514diguanylate cyclase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2212HTHFIS692e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 68.7 bits (168), Expect = 2e-14
Identities = 36/199 (18%), Positives = 70/199 (35%), Gaps = 19/199 (9%)

Query: 255 KILLVDDQQSMVDYFSSLLRSHGLMVKGMTKPEQVLPTLEQFEPDLFIFDLYMPDVNGLE 314
IL+ DD ++ + L G V+ + + + + DL + D+ MPD N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 315 LAKMIRQLDKYSSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP--SLFVTQVISRAQ 372
L I++ P+LV+S+ +T + + G+ D + K + +
Sbjct: 65 LLPRIKKARPD--LPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 373 RGHDIRSSASRDSLTGLLNHTQILVAARRCYNLAKRINSSVCIAMLDLDHFKQVNDTYGH 432
+ + L+ + + R + + ++ I G
Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMIT--------------GE 168

Query: 433 SGGDKVLLAFAHLLQQSLR 451
SG K L+A A L R
Sbjct: 169 SGTGKELVARA-LHDYGKR 186



Score = 53.7 bits (129), Expect = 1e-09
Identities = 27/123 (21%), Positives = 55/123 (44%), Gaps = 1/123 (0%)

Query: 131 RIAIVEDDSNVGAMITKQLHEFGFNVQHFLNFTDFLGIQNTSPFDLVLLDLILPDYTEAA 190
I + +DD+ + ++ + L G++V+ N DLV+ D+++PD
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 191 LFTAATEFEKNNTRVFVLSSRGDFEMRLLAIRANVSEYFVKPAETTLLVRKIHQWLKMSE 250
L + + + V V+S++ F + A +Y KP + T L+ I + L +
Sbjct: 65 LLPRIKK-ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 251 KQP 253
++P
Sbjct: 124 RRP 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2213HTHFIS665e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 5e-14
Identities = 29/107 (27%), Positives = 49/107 (45%), Gaps = 7/107 (6%)

Query: 3 IKVLVVDDSALIRNLLGKMIE-ADPELSLVGMAADAYMAKDMVNQHRPDVITLDIEMPKV 61
+LV DD A IR +L + + A ++ + AA + D++ D+ MP
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATL---WRWIAAGDGDLVVTDVVMPDE 60

Query: 62 DGLTFLDRLMKARPTAVVMISSLTEEG-ADATFNALGLGAVDFIPKP 107
+ L R+ KARP V++ ++ + A GA D++PKP
Sbjct: 61 NAFDLLPRIKKARPDLPVLV--MSAQNTFMTAIKASEKGAYDYLPKP 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2218PF06580397e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 38.7 bits (90), Expect = 7e-05
Identities = 15/70 (21%), Positives = 32/70 (45%), Gaps = 10/70 (14%)

Query: 420 EIDKGMIEKLVDPLT--HLVRNSLDHGIEKPEKRVAAGKSEVGVLSLKASQRGGSIVIAV 477
+I+ +++ V P+ LV N + HGI + + G + LK ++ G++ + V
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQ--------LPQGGKILLKGTKDNGTVTLEV 296

Query: 478 HDDGGGLNRE 487
+ G +
Sbjct: 297 ENTGSLALKN 306


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2219HTHFIS851e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 84.9 bits (210), Expect = 1e-22
Identities = 30/122 (24%), Positives = 54/122 (44%), Gaps = 3/122 (2%)

Query: 1 MSK-KILIVDDSAAIRQMVEATLKSANYQVVLAKDGREALDLCGGQRFDFILTDQNMPRM 59
M+ IL+ DD AAIR ++ L A Y V + + D ++TD MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 DGLTLIKSLRGMSAFIRTPIVMLTTEAGEDMKAQGRAAGATGWMVKPFDPQKLLAITAKV 119
+ L+ ++ A P+++++ + + GA ++ KPFD +L+ I +
Sbjct: 61 NAFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 120 LG 121
L
Sbjct: 119 LA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2220HTHFIS692e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 68.7 bits (168), Expect = 2e-14
Identities = 28/104 (26%), Positives = 50/104 (48%)

Query: 8 ILVIEDDLVTNQIITAFIHSKGWGVITCCSLEEASEEIYQQNIELILLDYFLPDGSALTL 67
ILV +DD ++ + G+ V + I + +L++ D +PD +A L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 68 LEKLRYCESPVPVIVISADNEYQKILSCFRLGALDYIIKPINLE 111
L +++ +PV+V+SA N + + GA DY+ KP +L
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2222PREPILNPTASE300.018 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 29.8 bits (67), Expect = 0.018
Identities = 16/57 (28%), Positives = 24/57 (42%), Gaps = 5/57 (8%)

Query: 11 FAAVIGYFIGS---LVSFTLSTLVAVIIMLGWQFYRQSSASQPIPHSFATSAASGDW 64
A +G ++G + LS+LV + +G R S+PIP F A W
Sbjct: 218 LLAALGAWLGWQALPIVLLLSSLVGAFMGIGLILLRNHHQSKPIP--FGPYLAIAGW 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2224TCRTETB1343e-36 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 134 bits (338), Expect = 3e-36
Identities = 83/390 (21%), Positives = 172/390 (44%), Gaps = 15/390 (3%)

Query: 50 LDTTIANVALPHMQGSMGATQDQISWVLTSYIVAAAIFMPLTGFLTARIGRKRVFMWAVV 109
L+ + NV+LP + +WV T++++ +I + G L+ ++G KR+ ++ ++
Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87

Query: 110 GFTIASMLCGAAQNLEQIVLF-RLLQGVFGASLVPLSQSVLLDSYPPERHGSAMALWGVG 168
S++ + +++ R +QG A+ L V+ P E G A L G
Sbjct: 88 INCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSI 147

Query: 169 VMVGPILGPSLGGWLTEYYNWRWVFYINLPFGLLAWFGLAAYVKETPLDHSRKFDLLGFA 228
V +G +GP++GG + Y +W + + +P + + + + FD+ G
Sbjct: 148 VAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGII 205

Query: 229 MLSLAIGALQMLLDRGESLDWFSSREIVIEAIIAGMAFYLFIAHIFTHKHPFIEPGLFKD 288
++S+ I + F++ + I++ ++F +F+ HI PF++PGL K+
Sbjct: 206 LMSVGIVFFML----------FTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKN 255

Query: 289 RNFSVGLIFIFIIGIILLATMALLPPFMQTLLGYPVIDVGY-LLAPRGVGTMIAMMTVGK 347
F +G++ II + ++++P M+ + ++G ++ P + +I G
Sbjct: 256 IPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGI 315

Query: 348 LAGKVDVRYQIFLGLMLTILSLWEMTGFNTNITGWDIVRTGVIQGLGLGFIFVPLSTITF 407
L + Y + +G+ +S F T W + V GL F +STI
Sbjct: 316 LVDRRGPLYVLNIGVTFLSVSFLTA-SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVS 374

Query: 408 ATLAAKYRNEGTALFSLMRNIGSSIGISVV 437
++L + G +L + + GI++V
Sbjct: 375 SSLKQQEAGAGMSLLNFTSFLSEGTGIAIV 404


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2225RTXTOXIND861e-20 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 85.7 bits (212), Expect = 1e-20
Identities = 41/263 (15%), Positives = 83/263 (31%), Gaps = 29/263 (11%)

Query: 99 AKLAQVKTDLAVLKASYHEKQAEITLAETKLAFAEKEQKRQENLIGKHFVS-------ES 151
+ Q + +L +A A I E + +L+ K ++ E+
Sbjct: 200 NQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQEN 259

Query: 152 QLEDARQNTDIARQNIQTLQKDLHRIAESLGGSP-DFPIEQHPSYLEALAQLNE------ 204
+ +A + + ++ ++ ++ E F E + +
Sbjct: 260 KYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELA 319

Query: 205 -AKLDLSRVEIKAPVSGVVSQLP--KLGQYVNVGAIALALV-ADHALWIEANFTETDLTH 260
+ I+APVS V QL G V + +V D L + A D+
Sbjct: 320 KNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGF 379

Query: 261 VKPGQKVNIHIDTFPDN---HWQGTVESLSPATGAEFSLIPAQNATGNWVKIAQRVPVRI 317
+ GQ I ++ FP + G V++++ G + +
Sbjct: 380 INVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA-------IEDQRLGLVFNVIISIEENC 432

Query: 318 AIDTALPEAPLRAGLSAVVDIDT 340
PL +G++ +I T
Sbjct: 433 LST-GNKNIPLSSGMAVTAEIKT 454



Score = 54.4 bits (131), Expect = 3e-10
Identities = 22/138 (15%), Positives = 43/138 (31%), Gaps = 12/138 (8%)

Query: 50 VKADKVPVSAQVAGNVDSLYVVENQRVEKGKVLFRLDDAMFKVMVDKASAKLAQVKTDLA 109
+ V + V E + V KG VL +L + K + L Q + +
Sbjct: 92 HSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQT 151

Query: 110 VLKASY----HEKQAEITLAETK--LAFAEKEQKRQENLIGKHFVSESQLEDARQNTDIA 163
+ K E+ L + +E+E R +LI + Q +
Sbjct: 152 RYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLI------KEQFSTWQNQKYQK 205

Query: 164 RQNIQTLQKDLHRIAESL 181
N+ + + + +
Sbjct: 206 ELNLDKKRAERLTVLARI 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2227RTXTOXINA300.025 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 29.5 bits (66), Expect = 0.025
Identities = 11/39 (28%), Positives = 16/39 (41%)

Query: 95 LINSLISKITQLNQGTEAFSSTLADFGLQLQTKPDVCTL 133
LIN L+ + LN +FS L G L + +
Sbjct: 187 LINQLVDTVASLNNNVNSFSQQLNTLGSVLSNTKHLNGV 225


77Shewana3_2411Shewana3_2418N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2411-1183.344651OmpA/MotB domain-containing protein
Shewana3_24121171.737186two component transcriptional regulator
Shewana3_24130161.424131ATPase domain-containing protein
Shewana3_24141160.688562cystathionine beta-lyase
Shewana3_24150140.214311CreA family protein
Shewana3_2416-1130.188348putative chaperone
Shewana3_2417-114-1.079833polyphosphate kinase
Shewana3_2418-217-0.210277Ppx/GppA phosphatase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2411OMPADOMAIN721e-16 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 71.5 bits (175), Expect = 1e-16
Identities = 27/92 (29%), Positives = 42/92 (45%), Gaps = 2/92 (2%)

Query: 142 ELALGMNVQFRTGSSELESHFLPQLDNVAKVMKRSSESN--LELKGYADRRGDLAYNQAL 199
L +V F + L+ LD + + + + + GY DR G AYNQ L
Sbjct: 214 HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGL 273

Query: 200 SEQRLLEVRGYLIKQGVAPERITTQAFGARMP 231
SE+R V YLI +G+ ++I+ + G P
Sbjct: 274 SERRAQSVVDYLISKGIPADKISARGMGESNP 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2412HTHFIS644e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.1 bits (156), Expect = 4e-14
Identities = 29/134 (21%), Positives = 54/134 (40%), Gaps = 5/134 (3%)

Query: 3 RIAIVEDEAAIRENYKDVLQQHGYSVQTYADRPSAMLAFNTRLPDLAIIDIGLGNEIDGG 62
I + +D+AAIR L + GY V+ ++ + DL + D+ + +E
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE--NA 62

Query: 63 FMLCQSLRAMSSTLPIIFLTARDSDFDTVCGLRLGADDYLSKEVSFPH---LTARLAALF 119
F L ++ LP++ ++A+++ + GA DYL K + R A
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 120 RRSELATSQTPQEN 133
+R Q+
Sbjct: 123 KRRPSKLEDDSQDG 136


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2416SHAPEPROTEIN423e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 42.4 bits (100), Expect = 3e-06
Identities = 25/81 (30%), Positives = 41/81 (50%), Gaps = 11/81 (13%)

Query: 191 AAKRAGFVDVAFLFEPLAAGMDYEASLSADQTVLVVDVGGGTTDCSVVKMGPKHQASFDR 250
+A+ AG +V + EP+AA + +S +VVD+GGGTT+ +V+ +
Sbjct: 129 SAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN--------- 179

Query: 251 SADCLGHSGQRIGGNDLDIAL 271
+ S RIGG+ D A+
Sbjct: 180 --GVVYSSSVRIGGDRFDEAI 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2418SHAPEPROTEIN320.007 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 31.7 bits (72), Expect = 0.007
Identities = 17/36 (47%), Positives = 24/36 (66%)

Query: 137 NIVIDIGGGSTEVVLGQKNTPTYLSSLRCGCVSFNE 172
++V+DIGGG+TEV + N Y SS+R G F+E
Sbjct: 161 SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDE 196


78Shewana3_2653Shewana3_2660N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2653119-0.411029oligopeptide/dipeptide ABC transporter ATPase
Shewana3_2654221-0.641800ABC transporter-like protein
Shewana3_2655323-0.765877trans-2-enoyl-CoA reductase
Shewana3_2656323-1.035963molybdenum-pterin binding
Shewana3_2657529-1.308809PpiC-type peptidyl-prolyl cis-trans isomerase
Shewana3_2658427-1.214700histone family protein DNA-binding protein
Shewana3_2659325-0.959296Lon-A peptidase
Shewana3_2660331-1.726431ATP-dependent protease ATP-binding subunit ClpX
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2653HTHFIS310.010 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.010
Identities = 9/16 (56%), Positives = 14/16 (87%)

Query: 38 LVGESGSGRSLLARAI 53
+ GESG+G+ L+ARA+
Sbjct: 165 ITGESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2654HTHFIS290.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.020
Identities = 13/28 (46%), Positives = 16/28 (57%)

Query: 42 TLAIVGEAGSGKSTLARILVGAEPRSGG 69
TL I GE+G+GK +AR L R G
Sbjct: 162 TLMITGESGTGKELVARALHDYGKRRNG 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2657SHAPEPROTEIN300.025 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 30.1 bits (68), Expect = 0.025
Identities = 50/203 (24%), Positives = 83/203 (40%), Gaps = 37/203 (18%)

Query: 428 MFSRD---DVPTA------------LNKPDVV-----KAAFSDTVLRQGLNSE-VIELEP 466
MFS D D+ TA LN+P VV +A +V G +++ ++ P
Sbjct: 8 MFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAAVGHDAKQMLGRTP 67

Query: 467 NHVVVIR-MKEHHDAGTMPLAEVKADIAERLKQDQANEAARAKAQELMTQVKAGATDVSL 525
++ IR MK+ G + V + + + + + + ++ V GAT V
Sbjct: 68 GNIAAIRPMKD----GVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVER 123

Query: 526 TA--KTKLGRGAQDVD------AAIVGKAFQMPTPTATPVVDTVGLANGYAVIALDKVNA 577
A ++ G GA++V AA +G + T + VVD G AVI+L+ V
Sbjct: 124 RAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVY 183

Query: 578 AESV---SDELVNALKQRLNAQY 597
+ SV D A+ + Y
Sbjct: 184 SSSVRIGGDRFDEAIINYVRRNY 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2658DNABINDINGHU1188e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 118 bits (297), Expect = 8e-39
Identities = 52/88 (59%), Positives = 69/88 (78%)

Query: 2 NKSELIEKIASGADISKAAAGRALDSFIAAVTEGLKEGDKISLVGFGTFEVRERAERTGR 61
NK +LI K+A +++K + A+D+ +AV+ L +G+K+ L+GFG FEVRERA R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGQEIKIAAAKIPAFKAGKALKDAV 89
NPQTG+EIKI A+K+PAFKAGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2659PF05272330.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 32.7 bits (74), Expect = 0.006
Identities = 15/86 (17%), Positives = 35/86 (40%), Gaps = 6/86 (6%)

Query: 286 AEATVVRSYVDWMTSVPWSQRSKIKRDLAKAQEVLDTDHYGLEKVKDRILEYLAVQSRVR 345
A+ V + DW+ + W + ++++ L D+ +++ + V
Sbjct: 527 ADMNRVHPFRDWVKAQQWDEVPRLEKWLVHVLGKTPDDYKPRRLRYLQLVGKYILMGHVA 586

Query: 346 QLKGP------ILCLVGPPGVGKTSL 365
++ P + L G G+GK++L
Sbjct: 587 RVMEPGCKFDYSVVLEGTGGIGKSTL 612


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2660HTHFIS300.018 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.2 bits (68), Expect = 0.018
Identities = 14/70 (20%), Positives = 28/70 (40%), Gaps = 13/70 (18%)

Query: 64 KLPTPHELRAHLDDYVIGQDRAKKVLSVAVYNHYKRLRNSSPKDGVELGKSNILLIGPTG 123
+ P+ E + ++G+ A +Y RL + +++ G +G
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAA----MQEIYRVLARLMQT---------DLTLMITGESG 170

Query: 124 SGKTLLAETL 133
+GK L+A L
Sbjct: 171 TGKELVARAL 180


79Shewana3_2745Shewana3_2751N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_27450164.218697peptidase S8/S53 subtilisin kexin sedolisin
Shewana3_27460224.725450hypothetical protein
Shewana3_27471234.847064acetyl-CoA hydrolase/transferase
Shewana3_27480214.601781ABC transporter
Shewana3_27491195.163232ABC transporter-like protein
Shewana3_27503162.379227secretion protein HlyD family protein
Shewana3_27512161.164602TetR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2745SUBTILISIN1437e-41 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 143 bits (363), Expect = 7e-41
Identities = 70/210 (33%), Positives = 97/210 (46%), Gaps = 24/210 (11%)

Query: 126 AGMKVCIIDSGLDSSNPDFNWNNITG----DNDSGTGNWYQNGGPHGTHVAGTIGAADNN 181
G+KV ++D+G D+ +PD I G D+D G +++ HGTHVAGTI A +N
Sbjct: 41 RGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGDPEIFKDYNGHGTHVAGTIAATENE 100

Query: 182 IGVVGMAPGVPMHIVKVFNASGWGYSSDLAYAANKCSNAGAKIISMSLGGGAANNTEKNA 241
GVVG+AP + I+KV N G G + IISMSLGG A
Sbjct: 101 NGVVGVAPEADLLIIKVLNKQGSGQYDWIIQGIYYAIEQKVDIISMSLGGPEDVPELHEA 160

Query: 242 FDAFTAAGGLVVAAAGNDGNSVRS-----YPAGYPSVMMIGANDANNKIADFSQYPSCVS 296
A+ LV+ AAGN+G+ YP Y V+ +GA + + ++FS
Sbjct: 161 VKKAVASQILVMCAAGNEGDGDDRTDELGYPGCYNEVISVGAINFDRHASEFSNS----- 215

Query: 297 GRGKKAVNDDGICVEVTAGGVDTLSTYPAG 326
V++ A G D LST P G
Sbjct: 216 ----------NNEVDLVAPGEDILSTVPGG 235



Score = 53.3 bits (128), Expect = 9e-10
Identities = 19/70 (27%), Positives = 27/70 (38%), Gaps = 7/70 (10%)

Query: 447 YGFMSGTSMATPAVSGMAALVWSN-----HSQCTGTQIRNALKATAMDAGTVGKDNYFGY 501
Y SGTSMATP V+G AL+ T ++ L + G G
Sbjct: 237 YATFSGTSMATPHVAGALALIKQLANASFERDLTEPELYAQLIKRTIPLG--NSPKMEGN 294

Query: 502 GIVNAKAADA 511
G++ A +
Sbjct: 295 GLLYLTAVEE 304


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2748ABC2TRNSPORT382e-05 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 38.4 bits (89), Expect = 2e-05
Identities = 44/166 (26%), Positives = 79/166 (47%), Gaps = 23/166 (13%)

Query: 186 GVILTMTMVMFT----SAAIVREREQGNMEFLITTPVRPLELMLGKI----TPYVLVGFV 237
G++ T M T AA R Q E ++ T +R +++LG++ T L G
Sbjct: 72 GMVATSAMTAATFETIYAAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAG 131

Query: 238 QLAIILSAGH-----LLFAVPIRGGLDSIALAAMLFICASLTLGLVISTIAKTQLQSMQM 292
+ + G+ LL+A+P+ IAL + F +LG+V++ +A + +
Sbjct: 132 IGVVAAALGYTQWLSLLYALPV------IALTGLAFA----SLGMVVTALAPSYDYFIFY 181

Query: 293 TVFILLPSILLSGFMFPYEAMPVAAQWIAEALPATHFMRMSRAIVL 338
++ P + LSG +FP + +P+ Q A LP +H + + R I+L
Sbjct: 182 QTLVITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIML 227


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2750RTXTOXIND522e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.8 bits (124), Expect = 2e-09
Identities = 30/144 (20%), Positives = 50/144 (34%), Gaps = 9/144 (6%)

Query: 51 TVERDRLTLTAPVGELINQINVVEGQQVQAGEVLLELDSTAAKARLGQRQAELKQA---- 106
T + ++ +I V EG+ V+ G+VLL+L + A+A + Q+ L QA
Sbjct: 91 THSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ 150

Query: 107 ---QAKLEEAVTGARSEDIDKARAALDGANASVKEVQQNFERTQR--LFKTKVLSQADLD 161
Q E + + + Q K + +LD
Sbjct: 151 TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLD 210

Query: 162 AALAARDTSLAKQAEAEQSLRLLQ 185
A R T LA+ E R+ +
Sbjct: 211 KKRAERLTVLARINRYENLSRVEK 234



Score = 49.4 bits (118), Expect = 8e-09
Identities = 32/239 (13%), Positives = 73/239 (30%), Gaps = 30/239 (12%)

Query: 76 QQVQAGEVLLELDSTAAKARLGQRQAELKQAQAKLEEAVTGARSEDIDKARAALDGANAS 135
Q V EVL K + Q + Q + L+ + + A ++
Sbjct: 177 QNVSEEEVLRLTS--LIKEQFSTWQNQKYQKELNLD-----KKRAERLTVLARINRYENL 229

Query: 136 VKEVQQNFERTQRLFKTKVLS--------------QADLDAALAARDTSLAKQAEAEQSL 181
+ + + L + ++ +L + + ++ A++
Sbjct: 230 SRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEY 289

Query: 182 RLLQNGTRSEQLEQARAAVEAAMAGVAQEQKALKDLSLVAARS----AVVDTLPWRVGDR 237
+L+ ++E L++ R + + K + R+ V G
Sbjct: 290 QLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGV 349

Query: 238 VAAGSQLIGLLAIEHPY-VRVYLPATWLDRVKAGSQVKILVDG----RAQPIAGTVRNI 291
V L+ ++ + V + + + G I V+ R + G V+NI
Sbjct: 350 VTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNI 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2751HTHTETR685e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 67.7 bits (165), Expect = 5e-16
Identities = 26/155 (16%), Positives = 56/155 (36%), Gaps = 6/155 (3%)

Query: 31 SDARQRLIAAAVTLFSERSYPTVSTREIAREAEVDAALIRYYFDSKAGLFEQMVRETLEP 90
+ RQ ++ A+ LFS++ + S EIA+ A V I ++F K+ LF ++ +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 91 VIARLREISSAQAPND---IGELMQTYYRVMAPNLGLPRLIVRVLQEGDGTEAYRIMLSV 147
+ E + + + E++ L+ + + + ++
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 148 FEQILSLSRQWVESAL---VNAGLLKEGLDPNLVR 179
+ S +E L + A +L L
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAA 164


80Shewana3_2873Shewana3_2881N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_2873-2120.276161N-acetyltransferase GCN5
Shewana3_2874-2110.172163MgtE integral membrane protein
Shewana3_2875017-1.214186glutathione peroxidase
Shewana3_2876116-0.995386response regulator receiver protein
Shewana3_2877119-1.772659phosphate binding protein
Shewana3_2878010-1.616833PAS/PAC sensor signal transduction histidine
Shewana3_2879111-2.341120two component transcriptional regulator
Shewana3_2880110-2.152416porin
Shewana3_2881112-1.627444recombination associated protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2873SACTRNSFRASE270.024 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 27.2 bits (60), Expect = 0.024
Identities = 22/106 (20%), Positives = 36/106 (33%), Gaps = 14/106 (13%)

Query: 51 LEGEMVGCAAIKAGSGPVGELGYLVVSPLYRRRGIAQGLTLKRIEVAKAQGIALLFATIR 110
LE +G I++ + + V+ YR++G+ L K IE AK L +
Sbjct: 72 LENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQ 131

Query: 111 DENNASRVNLLKAGFHFWRNYLSIRGTGNTVGWYYLALQESVDIDG 156
D N S HF+ + +G L +
Sbjct: 132 D-INISAC-------HFYAKH------HFIIGAVDTMLYSNFPTAN 163


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2878PF06580330.002 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.002
Identities = 20/106 (18%), Positives = 34/106 (32%), Gaps = 26/106 (24%)

Query: 327 LISNAIRY----TEPGGKITVQWRSVATGGLFSVTDTGEGIAPQHISRLTERFYRVDSAR 382
L+ N I++ GGKI ++ V +TG
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKN---------------- 306

Query: 383 SRQTGGSGLGLAIVKHALSHHHSE---LNISSELGKGSTFSFVIPS 425
+G GL V+ L + + +S + GK + +IP
Sbjct: 307 --TKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM-VLIPG 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2879HTHFIS912e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 91.4 bits (227), Expect = 2e-23
Identities = 34/130 (26%), Positives = 64/130 (49%), Gaps = 4/130 (3%)

Query: 3 ARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGSGI 62
A IL+ +D+ AIR +L + + G+ + + + DL++ D + P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 63 QLAKRLKQDEFTRQIPIIMLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVL-- 120
L R+K+ +P+++++A+ +K E GA DY+ KPF EL+ I L
Sbjct: 64 DLLPRIKKAR--PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 121 RRSAPTRLEE 130
+ P++LE+
Sbjct: 122 PKRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2880ECOLNEIPORIN811e-19 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 81.0 bits (200), Expect = 1e-19
Identities = 79/335 (23%), Positives = 126/335 (37%), Gaps = 33/335 (9%)

Query: 7 KTLLASALASATLASAYAAEPLTVYGKLNV---TAQSNDEKGDST------TTIQSNASR 57
K+L+A LA+ +A A +T+YG + T++S G T I S+
Sbjct: 3 KSLIALTLAALPVA---AMADVTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLGSK 59

Query: 58 FGVKGDFELSSSLEAFYTVEYEVDTGAATSDNFKARNQFVGLKGAFGSFSVGRNDTLLKI 117
G KG +L + L+A + VE + A T + R F+GLKG FG VGR +++LK
Sbjct: 60 IGFKGQEDLGNGLKAIWQVEQKASI-AGTDSGWGNRQSFIGLKGGFGKLRVGRLNSVLK- 117

Query: 118 SQGNVDQFNDLSGDL--KSLFKGENRLGQTATYLSPSIGGFVFGATYAAEGDADQQAQDG 175
G+++ ++ S L + + E RL + Y SP G YA +A + +
Sbjct: 118 DTGDINPWDSKSDYLGVNKIAEPEARL-ISVRYDSPEFAGLSGSVQYALNDNAGRHNSES 176

Query: 176 FSLAAMYGDAKLKKSPFYAAIAYDSDVKGYEILRASVQGKI----ADLTLGGMYQQQEQT 231
+ Y + A + + I + + + D + QQ+
Sbjct: 177 YHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIEKYQIHRLVSGYDNDALYASVAVQQQDA 236

Query: 232 YKNSLPVNTDSVNGYLLSAAYDINAVTLKAQY----------QDMEDLGDSWSVGADYSL 281
+ +S + AY VT + Y + + D VGA+Y
Sbjct: 237 KLVEENYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDATNYNNDYDQVVVGAEYDF 296

Query: 282 GKPTKVFAFYT--NRSMEASNDDDKYIGVGLEHKF 314
K T S GVGL HKF
Sbjct: 297 SKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2881SECA310.006 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 31.0 bits (70), Expect = 0.006
Identities = 10/41 (24%), Positives = 25/41 (60%), Gaps = 1/41 (2%)

Query: 81 ESLEEKVAQIEDEENRKLAKKEKDALKD-EIITSLLPRAFS 120
++E ++ ++ DEE + + + L+ E++ +L+P AF+
Sbjct: 29 NAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFA 69


81Shewana3_2980Shewana3_2988N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_29800152.255963TetR family transcriptional regulator
Shewana3_2981-1142.556280HPP family protein
Shewana3_2982-2132.560189short chain fatty acid transporter
Shewana3_2983-2132.279116peptidylprolyl isomerase, FKBP-type
Shewana3_2984-1132.151471glycosyl hydrolase family chitinase
Shewana3_2985-1130.783868ROK family protein
Shewana3_2986-2130.569045peptidase M24
Shewana3_2987-113-0.271210methyl-accepting chemotaxis sensory transducer
Shewana3_2988013-1.231286DEAD/DEAH box helicase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2980HTHTETR551e-11 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.0 bits (132), Expect = 1e-11
Identities = 21/134 (15%), Positives = 44/134 (32%), Gaps = 4/134 (2%)

Query: 13 DKRQQLISTAFKLFYFQSVHGVGINQILQESAIAKKTLYHHFASKDELVEAVVLYRDRVF 72
+ RQ ++ A +LF Q V + +I + + + + +Y HF K +L + +
Sbjct: 11 ETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNI 70

Query: 73 YQWLSERVLAV-EMGTAGIRVLFMALDDWFNQRVPQLCEFRGCFFINVSAEFTDASHPVH 131
+ E + +R + + + + + F EF V
Sbjct: 71 GELELEYQAKFPGDPLSVLREILIHVLESTVTE-ERRRLLMEIIF--HKCEFVGEMAVVQ 127

Query: 132 RLCAEHKQRVADLI 145
+ D I
Sbjct: 128 QAQRNLCLESYDRI 141


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2983INFPOTNTIATR1373e-43 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 137 bits (345), Expect = 3e-43
Identities = 65/132 (49%), Positives = 88/132 (66%), Gaps = 2/132 (1%)

Query: 25 KAAQENIRLGNEFLAQNKNQEGVKTTASGLQYQVLQQGTGTVHPKASDTVTVHYHGTLID 84
K A+EN G+ FL+ NK++ G+ SGLQY+++ GTG P SDTVTV Y GTLID
Sbjct: 99 KKAEENKAKGDAFLSANKSKPGIVVLPSGLQYKIIDAGTGA-KPGKSDTVTVEYTGTLID 157

Query: 85 GTVFDSSVERGEPIAFPLNRVIKGWTEGVQLMVEGDKYRFFIPSELAYGNRST-GKIGGG 143
GTVFDS+ + G+P F +++VI GWTE +QLM G + F+P++LAYG RS G IG
Sbjct: 158 GTVFDSTEKAGKPATFQVSQVIPGWTEALQLMPAGSTWEVFVPADLAYGPRSVGGPIGPN 217

Query: 144 SVLIFDVELLKI 155
LIF + L+ +
Sbjct: 218 ETLIFKIHLISV 229


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2984MICOLLPTASE465e-07 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 46.2 bits (109), Expect = 5e-07
Identities = 33/170 (19%), Positives = 65/170 (38%), Gaps = 15/170 (8%)

Query: 542 WEIDADNGDILNAMHEGLGHGEGTMPPANKAPIANAGADITVTGTADVTLNGSASRDPEN 601
++D + + + + G+ T NK P A +D +V ++ +G+ S+D +
Sbjct: 744 HKVDGNGNYVYDVVFHGMNTDTNTDVHVNKEPKAVIKSDSSVIVEEEINFDGTESKDEDG 803

Query: 602 GALSYQWSQVSGPSLSISNADMANAVVQLSEAASDVVYVFSLQVTDPEGLSSTDTVTLTH 661
+Y+W G ++ A A + ++ Y L VTD G +T++ +
Sbjct: 804 EIKAYEWDFGDG-----EKSNEAKATHKYNKTGE---YEVKLTVTDNNGGINTESKKI-- 853

Query: 662 KAETANQAPVV--TVPASVTVEAGQSVSINATAT---DADGDSLTYAWTV 706
K V+ + P + +A Q N + S Y + V
Sbjct: 854 KVVEDKPVEVINESEPNNDFEKANQIAKSNMLVKGTLSEEDYSDKYYFDV 903


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2987FLAGELLIN320.008 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 31.6 bits (71), Expect = 0.008
Identities = 14/87 (16%), Positives = 33/87 (37%), Gaps = 4/87 (4%)

Query: 282 QLASAMEEMSSTIAEVAQNTQLTSTSINTAYDLCLKSSANMKANTQKVEQLAKSVADAAN 341
+A+ + + ++N + T + + N Q+V +L+ + N
Sbjct: 48 AIANRFTSNIKGLTQASRNANDGISIAQTTE----GALNEINNNLQRVRELSVQATNGTN 103

Query: 342 NAHQLNKEAERVASAMGEIDSIAEQTN 368
+ L + + + EID ++ QT
Sbjct: 104 SDSDLKSIQDEIQQRLEEIDRVSNQTQ 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_2988SECA330.004 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 32.5 bits (74), Expect = 0.004
Identities = 37/175 (21%), Positives = 67/175 (38%), Gaps = 46/175 (26%)

Query: 220 SQVVYPVEQRRKRELLSELIGK-KNWQQVLVFTATRDAADTLVKELNLDGIPSEVVHGEK 278
+VY E + + ++ ++ + Q VLV T + + ++ + EL GI V++
Sbjct: 424 PDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLN--- 480

Query: 279 AQGSRRRALREFVSGKVR---VLVATEVAARGLDI---------------PSLEYVVNFD 320
A+ A V+ V +AT +A RG DI P+ E +
Sbjct: 481 AKFHANEA--AIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIK 538

Query: 321 LPFLAED---------YV-----H---RI-----GRTGRAGKSGVAISFVSREEE 353
+ ++ H RI GR+GR G +G + ++S E+
Sbjct: 539 ADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDA 593


82Shewana3_3193Shewana3_3198N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3193-1131.219718secretion protein HlyD family protein
Shewana3_31940131.651331hypothetical protein
Shewana3_31950120.974827hypothetical protein
Shewana3_31961131.691913ATPase central domain-containing protein
Shewana3_31970141.690000phosphoribosylglycinamide formyltransferase 2
Shewana3_31980131.258954hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3193RTXTOXIND546e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 53.7 bits (129), Expect = 6e-10
Identities = 46/320 (14%), Positives = 95/320 (29%), Gaps = 80/320 (25%)

Query: 66 ITPAVKGLVISVEAKPNTPMKQGDVLFRIDPTPFEAVVKRKKAALLAAEQEVPQLAAAWE 125
I P +V + K +++GDVL ++ EA + +++LL A E +
Sbjct: 99 IKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSR 158

Query: 126 AAKANV---------ARVAADRERNKSAY--------------------DRYEQGHRKGG 156
+ + N E + ++ +
Sbjct: 159 SIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLT 218

Query: 157 ANSPFTALE---------LDNRRQLF---FASEAQLTAAQAE--ELRARLA-YESNVDGV 201
+ E LD+ L ++ + + + E L Y+S ++ +
Sbjct: 219 VLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQI 278

Query: 202 NSKVAGLQGDL-----------------------------ESALYNLEQTVVRAPADGIV 232
S++ + + + +V+RAP V
Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKV 338

Query: 233 TQMALR-PGAMAVPLPLRPVMSFIPDEQRYFAGAFWQNSLL-RLQEGDEAEVVLDAAPGQ 290
Q+ + G V +M +P++ A QN + + G A + ++A P
Sbjct: 339 QQLKVHTEG--GVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYT 396

Query: 291 ---VFKGKVAKVLPAMAEGE 307
GKV + E +
Sbjct: 397 RYGYLVGKVKNINLDAIEDQ 416


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3196HTHFIS290.039 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.4 bits (66), Expect = 0.039
Identities = 55/293 (18%), Positives = 102/293 (34%), Gaps = 66/293 (22%)

Query: 272 RAPATQATTDDKATVQP-DAPKGVLLLGVQGSGKSLAAKAV---AGVWQRPLLRLDMGAL 327
R+ A Q A + D +++ G G+GK L A+A+ P + ++M A+
Sbjct: 142 RSAAMQEIYRVLARLMQTDLT--LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAI 199

Query: 328 YNKYIGETE-------------KNLRNALELADMMSPCILWIDEIEKGLSGSSS------ 368
I E+E E A+ + L++DEI + +
Sbjct: 200 PRDLI-ESELFGHEKGAFTGAQTRSTGRFEQAEGGT---LFLDEIGD-MPMDAQTRLLRV 254

Query: 369 -DEGTSTRILGTLLTWMAERKSEVFVVATANDIQVLPPELMRKGRMDE-------IFFVD 420
+G T + G +S+V +VA N + + +G E + +
Sbjct: 255 LQQGEYTTVGGR-----TPIRSDVRIVAATN---KDLKQSINQGLFREDLYYRLNVVPLR 306

Query: 421 LPDEAIRQAI---------FLIHCQRRGIDVTRLD---LAQLSRHSQGFSG--AEIEQAV 466
LP +R F+ ++ G+DV R D L + H + G E+E V
Sbjct: 307 LP--PLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHP--WPGNVRELENLV 362

Query: 467 --IAAMYSARSLARSLDQDMLLEELTKTKPLSIVMGDKINALRQWAAGRTVNA 517
+ A+Y + R + ++ L E+ + ++ Q
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQY 415


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3197PF06057310.005 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 31.3 bits (71), Expect = 0.005
Identities = 10/28 (35%), Positives = 13/28 (46%), Gaps = 2/28 (7%)

Query: 17 GCGELGKEVAIELQRLGVEVIGVD--RY 42
G L K V LQ+ G V+G +Y
Sbjct: 62 GWATLDKAVGGILQQQGWPVVGWSSLKY 89


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3198BCTERIALGSPC320.006 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 31.9 bits (72), Expect = 0.006
Identities = 23/112 (20%), Positives = 40/112 (35%), Gaps = 20/112 (17%)

Query: 372 GMIWFRLPLEGDKRVWPLSTLIAVAQQQPLAPHIELEIL--------NQANTEPAQHEAP 423
MI++R+ L + V + A A+QQP+ + + + A P
Sbjct: 31 AMIFWRIGLPDNAPVSSVQITPAQARQQPVTLN-DFTLFGVSPEKNKAGALDASQMSNLP 89

Query: 424 KTSLFQLVLVNKGNLAGELPGQLSLAAQACSGY-------DAQNGYQAKLTQ 468
+ L L G +AG+ S+A + + GY AK+
Sbjct: 90 PS---TLNLSLTGVMAGDDD-SRSIAIISKDNEQFSRGVNEEVPGYNAKIVS 137


83Shewana3_3334Shewana3_3338N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3334-1131.524482response regulator receiver modulated metal
Shewana3_3335-1151.704538multi-sensor hybrid histidine kinase
Shewana3_3336-1181.550960ATP-dependent RNA helicase SrmB
Shewana3_3337-1181.199906RND family efflux transporter MFP subunit
Shewana3_3338-1191.567627acriflavin resistance protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3334HTHFIS816e-19 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 80.6 bits (199), Expect = 6e-19
Identities = 31/169 (18%), Positives = 60/169 (35%), Gaps = 23/169 (13%)

Query: 10 TLLLVDDEPVNLRVLKQVLHQ-DYHLIFAKSGEEALRLAQTELPSLILLDIMMPNMTGLE 68
T+L+ DD+ VL Q L + Y + + R L++ D++MP+ +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 69 VCQLLKNISETQSIPVIFVTALNDEHDEAAGFAVGGVDYIVKPISATIVKARVKTHLSLV 128
+ +K +PV+ ++A N G DY+ KP T + + L+
Sbjct: 65 LLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 129 QADELRRTR---------------LQVIQRLGRAAEYKDN-----ETGT 157
+ + ++ + L R + E+GT
Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGT 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3335HTHFIS642e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.1 bits (156), Expect = 2e-12
Identities = 27/122 (22%), Positives = 46/122 (37%), Gaps = 5/122 (4%)

Query: 789 TLLVVDDIQQNIDLLSVWLTRQGHKVITARDGEQALLRMQKADIDITLMDLQMPVMDGLT 848
T+LV DD +L+ L+R G+ V + + D D+ + D+ MP +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 849 AAKMRREQEAESQLPHMPIIALTASVLEQDKSAAEQAGMDGFANKPIDFALLTREIARVL 908
+ P +P++ ++A A + G + KP D L I R L
Sbjct: 65 L-----LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 909 QL 910

Sbjct: 120 AE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3337RTXTOXIND424e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 4e-06
Identities = 24/121 (19%), Positives = 47/121 (38%), Gaps = 7/121 (5%)

Query: 106 DYEADLMQAEATLAQATAALNEEIARGEVAKIEFKGYDKGLPPELGLRIPQLKKEQANVK 165
+ E ++A L + L + + AK E++ + E+ + +L++ N+
Sbjct: 256 EQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEI---LDKLRQTTDNIG 312

Query: 166 YAQAALARAQRNLERTVIRAPFDGIIKARNV-DLGQYVTLGTNLGELY---DTSIAEIRL 221
LA+ + + +VIRAP ++ V G VT L + DT +
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 222 P 222

Sbjct: 373 Q 373



Score = 37.9 bits (88), Expect = 6e-05
Identities = 25/125 (20%), Positives = 53/125 (42%), Gaps = 11/125 (8%)

Query: 62 GVVTPKYKTQLVTEVQGRMLSISPQFVA-GGIVKKGDQLAQIEPSDYEADLMQAEATLAQ 120
G +T +++ + ++ + + V G V+KGD L ++ EAD ++ +++L Q
Sbjct: 88 GKLTHSGRSKEIKPIENSI--VKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQ 145

Query: 121 ATA------ALNEEIARGEVAKIEFKGYD--KGLPPELGLRIPQLKKEQANVKYAQAALA 172
A L+ I ++ +++ + + E LR+ L KEQ + Q
Sbjct: 146 ARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQK 205

Query: 173 RAQRN 177
+
Sbjct: 206 ELNLD 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3338ACRIFLAVINRP498e-161 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 498 bits (1283), Expect = e-161
Identities = 213/1043 (20%), Positives = 448/1043 (42%), Gaps = 49/1043 (4%)

Query: 11 FARNSVAANLLMWALLIGGLFSTVLINKEVFPSFNLNLLSITVAYPGAAPQEIEEGINIK 70
F R + A +L L++ G + + + +P+ +S++ YPGA Q +++ +
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 71 IEEAIQDINGIKKVTSVA-SEGVGAITVEVEDDYDVQTVLDEAKLRLDAI-STFPVNIEK 128
IE+ + I+ + ++S + S G IT+ + D + + +L P +++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 129 PQIFKIEPENNVIWV----SVYGDMSLHDMKELAKS-VRDDLTQLPSVTRAKVTGVRDYE 183
I + ++ + V S + D+ + S V+D L++L V ++ G Y
Sbjct: 125 QGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG-AQYA 183

Query: 184 IGIEVSEDKLREYGLTFSQVALAVQNSSIDLPGGSIRAEDG------DILLRTKGQAYTG 237
+ I + D L +Y LT V ++ + + G + + + + +
Sbjct: 184 MRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNP 243

Query: 238 DDFANIVVTTRTDGSRVMLPQVATIKDDFEERLEYTRFNGKPAAIIEVTSVNDQNALDIA 297
++F + + +DGS V L VA ++ E R NGKPAA + + NALD A
Sbjct: 244 EEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDTA 303

Query: 298 QQVKDYVEKRRATLPANAQLDTWGDLTHYLKGRLNMMMSNMFYGALLVFVILALFL-DLK 356
+ +K + + + P ++ D T +++ ++ ++ +F +LVF+++ LFL +++
Sbjct: 304 KAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMR 363

Query: 357 LAFWVMMGLPVCFLGTMLIMPLEPFSMTINMLTLFAFILVLGIVVDDAIVIGESAYSE-V 415
+ +PV LGT I+ F +IN LT+F +L +G++VDDAIV+ E+ +
Sbjct: 364 ATLIPTIAVPVVLLGTFAILAA--FGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 416 ERHGHSIDNVIRGAQKVAMPATFGVLTTIAAFIPMLMVSGPMGIIWKSIGMVVIMCLAFS 475
E + + ++ + A FIPM G G I++ + ++ +A S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 476 LVESKFILPAHLAHM-KFRKPGE---PTGFFGRFKDRFNNHVQHFIHHSYRNFLERCIQH 531
++ + + PA A + K GFFG F F++ V H+ +S L
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYT-NSVGKILGSTG-- 538

Query: 532 RYNVVAAFIGVLILSIALVASGKVRWVFFPDIPSDFIQVQLEMDEGSSEQNTLKVVQDIE 591
RY ++ A I ++ + L ++ F P+ +++ G++++ T KV+ +
Sbjct: 539 RYLLIYALIVAGMVVLFL----RLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVT 594

Query: 592 EALYKMNAKMEKDNGSEVVKHSFINMSSRTSAFIFAELTKGEDRDVDGET---IAAAWRE 648
+ K + + V SF ++ + F L E+R+ D + + +
Sbjct: 595 DYYLKNEKANVESVFT-VNGFSFSGQ-AQNAGMAFVSLKPWEERNGDENSAEAVIHRAKM 652

Query: 649 QLPELLSVKKLDFNAS-----GNGGGGGDISFRLTSSDLEELSAAARELKQKLATY-EGV 702
+L ++ + FN G G + L+ A +L A + +
Sbjct: 653 ELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASL 712

Query: 703 YDIADNFSSGSHEIRLKI-RPEAEALGLTLSDLARQVRYGFYGYEAQRILRNKEEIKVMV 761
+ N + + +L++ + +A+ALG++LSD+ + + G + K+ V
Sbjct: 713 VSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYV 772

Query: 762 RYPLEQRRTVGYLENMLIRTPQGKSVPFSTVAEVEKGESYASITRVDGKRAITIIANANK 821
+ + R ++ + +R+ G+ VPFS + R +G ++ I
Sbjct: 773 QADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGE--- 829

Query: 822 HKVEPSKVVNEIQQDFLPQLQAKYPK-IQTTLDGGSLDEQNAMVGLMQGFFFALFTIYAL 880
P + + L +K P I G S E+ + + ++
Sbjct: 830 --AAPGTSSGDA-MALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLC 886

Query: 881 MAVPLKSYSQPLIIMSVIPFGIIGALFGHLIQGLAMSVLSLCGIVALAGVVVNDSLILVD 940
+A +S+S P+ +M V+P GI+G L + V + G++ G+ +++++V+
Sbjct: 887 LAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVE 946

Query: 941 FVNRARE-QGQSVRQAAVDSGCYRFRAIILTSLTTFVGLVPIILERSLQAQIVIPMATSL 999
F E +G+ V +A + + R R I++TSL +G++P+ + + + +
Sbjct: 947 FAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGV 1006

Query: 1000 AFGILFSTVVTLILVPLLYIILD 1022
G++ +T++ + VP+ ++++
Sbjct: 1007 MGGMVSATLLAIFFVPVFFVVIR 1029


84Shewana3_3418Shewana3_3425N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3418-2152.433074response regulator receiver modulated metal
Shewana3_3419-1152.445670multi-sensor hybrid histidine kinase
Shewana3_34202193.176151amino acid carrier protein
Shewana3_34212202.818648ABC transporter-like protein
Shewana3_34222202.888984hypothetical protein
Shewana3_3423-1212.905539hypothetical protein
Shewana3_3424-1243.050293methylation site containing protein
Shewana3_3425-1212.840387methylation site containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3418HTHFIS904e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.9 bits (223), Expect = 4e-22
Identities = 39/159 (24%), Positives = 65/159 (40%), Gaps = 6/159 (3%)

Query: 1 MDKATILVVDDTPENIDILVGILG-EDYKVKVAIDGPRALALVAKTLPDLILLDVMMPGM 59
M ATILV DD +L L Y V++ + +A DL++ DV+MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 NGYEVCKLLKQEPLTCHIPVIFVTALSEVADETQGFELGAVDYITKPVSAPVVKARVRTH 119
N +++ +K+ +PV+ ++A + + E GA DY+ KP + +
Sbjct: 61 NAFDLLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 120 LALYDQKRLLEQQVKERTQEL--EETRF-EIIRRLGRAA 155
LA ++ + + L EI R L R
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLM 157


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3419HTHFIS773e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.8 bits (189), Expect = 3e-16
Identities = 34/128 (26%), Positives = 54/128 (42%), Gaps = 5/128 (3%)

Query: 1284 ILVADDNATARDIMRTTLESMGFRVDTVRSGEEAVTRCIQQEYAVALIDWKMPNLDGIET 1343
ILVADD+A R ++ L G+ V + + + + D MP+ + +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 1344 AKQIKQQAKKAPRILMVSAHANQDFLSQIEELGLAGYISKPISASRLLDGIINSLGRAGV 1403
+IK+ P ++M SA + E G Y+ KP L +I +GRA
Sbjct: 66 LPRIKKARPDLPVLVM-SAQNTFMTAIKASEKGAYDYLPKPFD----LTELIGIIGRALA 120

Query: 1404 LPVRRQSE 1411
P RR S+
Sbjct: 121 EPKRRPSK 128



Score = 67.5 bits (165), Expect = 2e-13
Identities = 24/103 (23%), Positives = 42/103 (40%), Gaps = 2/103 (1%)

Query: 1425 RILLVEDNEMNLEVATEFLEQVGIILSIATNGQIALDKLEQQSFDLVLMDCQMPVMDGYQ 1484
IL+ +D+ V + L + G + I +N + DLV+ D MP + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 1485 ATQALRKRPELTELPVVAMTANAMAGDKEMCLRAGMNDHIAKP 1527
++K +LPV+ M+A G D++ KP
Sbjct: 65 LLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKP 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3424BCTERIALGSPG533e-12 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 53.4 bits (128), Expect = 3e-12
Identities = 19/64 (29%), Positives = 38/64 (59%)

Query: 5 RKGFTLIELMIAVAIIGILAAIAIPSFNEYLKQGRRFDAQQYLVSSAQALERHYSRNGLY 64
++GFTL+E+M+ + IIG+LA++ +P+ ++ + A +V+ AL+ + N Y
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHHY 66

Query: 65 PASQ 68
P +
Sbjct: 67 PTTN 70


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3425BCTERIALGSPG391e-06 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 39.1 bits (91), Expect = 1e-06
Identities = 15/31 (48%), Positives = 23/31 (74%)

Query: 2 AKRTKAGFTLVELLVAIAIIGILASIALPSY 32
A + GFTL+E++V I IIG+LAS+ +P+
Sbjct: 3 ATDKQRGFTLLEIMVVIVIIGVLASLVVPNL 33


85Shewana3_3454Shewana3_3466N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3454-1142.409839outer membrane efflux protein
Shewana3_3455-2142.288562ABC transporter-like protein
Shewana3_3456-2151.397963RND family efflux transporter MFP subunit
Shewana3_3457-2141.337600PHB depolymerase family esterase
Shewana3_3458-2131.168530TonB-dependent receptor
Shewana3_3459-2152.746645two component LuxR family transcriptional
Shewana3_3460-2133.173675multi-sensor hybrid histidine kinase
Shewana3_34610133.427434major facilitator superfamily transporter
Shewana3_3462-1123.852571alpha/beta hydrolase domain-containing protein
Shewana3_34630123.783683hypothetical protein
Shewana3_3464-1134.178022amidase
Shewana3_3465-1123.013661agmatine deiminase
Shewana3_3466-1142.317779short-chain dehydrogenase/reductase SDR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3454RTXTOXIND371e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 37.1 bits (86), Expect = 1e-04
Identities = 24/186 (12%), Positives = 53/186 (28%), Gaps = 5/186 (2%)

Query: 74 QPELNQLISQVLSSNNDLTLATLTLQKARLQAGLARDDLYPQLSSNNTASVNKPLDGGSS 133
+P N ++ +++ + L +L A A D SS A + + S
Sbjct: 100 KPIENSIVKEIIVKEGESVRKGDVL--LKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 134 SRAFQANL-SVSYEVDLWGKVSANIDQAQWTALAS--LEDRESTAQSLVATTASLYWQIG 190
L + + + + + + + T+L ++ +
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 191 YLHERIELSNKSIEHSRQTLALTQRQYASGAVTELNVLESQRSLAGQEASHSQLLQQLVE 250
+ RI + L A+ + VLE + QL +
Sbjct: 218 TVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQ 277

Query: 251 AENALA 256
E+ +
Sbjct: 278 IESEIL 283


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3456RTXTOXIND642e-13 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 64.5 bits (157), Expect = 2e-13
Identities = 36/199 (18%), Positives = 67/199 (33%), Gaps = 26/199 (13%)

Query: 58 ANGMLQASKLVSVGAQVSGQIQSLPV------DLGQEVKKGDLIAQIDSLAQQNNLQNAL 111
N + ++ ++V A+++ V D + K IA+ L Q+N
Sbjct: 207 LNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQ-AIAKHAVLEQEN----KY 261

Query: 112 ASLKSINAQYRAKQAQIRQAKLEYTRQQEMLADKASSRADFETAEATLTVYQAELEQLQA 171
+ Y+++ QI L + +++ F+ +L Q
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLV------TQLFKNEILD------KLRQTTD 309

Query: 172 QKQQAEINVDSARIDLGYTKITAPMDGTVVYSAV-EVGQTVNANQTTPTIVEMAQLDTMT 230
+ + + I AP+ V V G V +T IV + DT+
Sbjct: 310 NIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIV--PEDDTLE 367

Query: 231 VKAQISEADIVNVHPGQAV 249
V A + DI ++ GQ
Sbjct: 368 VTALVQNKDIGFINVGQNA 386



Score = 48.3 bits (115), Expect = 3e-08
Identities = 31/182 (17%), Positives = 70/182 (38%), Gaps = 15/182 (8%)

Query: 7 MKKSSKRKLILILSGLVLLGGGAYFLLHKPEAAPSYVTEPVKRGDIENSVLANGMLQAS- 65
++ R+ L+ ++G + S + + +E ANG L S
Sbjct: 49 IETPVSRRPRLV--AYFIMGFLVIAFIL------SVLGQ------VEIVATANGKLTHSG 94

Query: 66 KLVSVGAQVSGQIQSLPVDLGQEVKKGDLIAQIDSLAQQNNLQNALASLKSINAQYRAKQ 125
+ + + ++ + V G+ V+KGD++ ++ +L + + +SL + Q
Sbjct: 95 RSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQ 154

Query: 126 AQIRQAKLEYTRQQEMLADKASSRADFETAEATLTVYQAELEQLQAQKQQAEINVDSARI 185
R +L + ++ + E ++ + + Q QK Q E+N+D R
Sbjct: 155 ILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRA 214

Query: 186 DL 187
+
Sbjct: 215 ER 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3459HTHFIS642e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.5 bits (157), Expect = 2e-14
Identities = 40/167 (23%), Positives = 68/167 (40%), Gaps = 8/167 (4%)

Query: 1 MSQIKVAIADDHPLFRTALTQAVLKNVNTAEVLEAENFQELITLVENNPDIELIFLDLHM 60
M+ + +ADD RT L QA L +V N L + +L+ D+ M
Sbjct: 1 MTGATILVADDDAAIRTVLNQA-LSRAG-YDVRITSNAATLWRWIAAGD-GDLVVTDVVM 57

Query: 61 PGNEGFTGLTLLQNHFPDIAVIMVSSDDQPEIIRKAINFGASAFIPKSASLTQISTAIAT 120
P F L ++ PD+ V+++S+ + KA GA ++PK LT++ I
Sbjct: 58 PDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117

Query: 121 VLEGEVWLPEHTDINVDQQ-----TAAEHQRLAKQLAQLTPQQYTVL 162
L P + + +A Q + + LA+L T++
Sbjct: 118 ALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLM 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3460HTHFIS399e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 39.4 bits (92), Expect = 9e-05
Identities = 14/71 (19%), Positives = 30/71 (42%), Gaps = 2/71 (2%)

Query: 1055 ISVLVIDNDELMLKAISSLLLGWGCHVLTARDKASAEQQLTQQVLPKLIIADYHLDDDQN 1114
++LV D+D + ++ L G V + A+ + + L++ D + D+ N
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWI-AAGDGDLVVTDVVMPDE-N 61

Query: 1115 GVDLVQSLLTH 1125
DL+ +
Sbjct: 62 AFDLLPRIKKA 72


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3461TCRTETB290.024 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.5 bits (66), Expect = 0.024
Identities = 27/107 (25%), Positives = 40/107 (37%), Gaps = 4/107 (3%)

Query: 252 GIVGTIAGILYSRKQPLRLPIIRLSGLLIFLTVLGLSFGTAPWLQTLCAI-VLGFCIFLP 310
I G I GIL R+ PL + I ++ L + T W T+ + VLG F
Sbjct: 307 IIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTK 366

Query: 311 VTALVSIPHELPKMTSQKITVIFSLFWSISYLISTLVLWLFGKLVDI 357
+ L Q+ SL S+L + + G L+ I
Sbjct: 367 TVISTIVSSSL---KQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSI 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3466DHBDHDRGNASE1171e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 117 bits (294), Expect = 1e-33
Identities = 74/258 (28%), Positives = 120/258 (46%), Gaps = 6/258 (2%)

Query: 34 LKGKVGLITGSTSGIGLATAHVLAEQGCHLILHGLMPEAEGRRLAAEFAEQYHIHTYFSN 93
++GK+ ITG+ GIG A A LA QG H+ PE + +++ AE H +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF--P 63

Query: 94 ADLRDPESIHAFMDAGVKALGSIDILVNNAGIQHTENVAHFPIDKWNDIIAINLSSAFHT 153
AD+RD +I + +G IDILVN AG+ + ++W ++N + F+
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 154 IQQAVPAMAEKRWGRIINIASVHGLVASVNKAAYCAAKHGIVGLTKVVAIECAEQGITVN 213
+ M ++R G I+ + S V + AAY ++K V TK + +E AE I N
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 214 AICPGWVDTPLINK-QIEAIASNKGLSYDEAKYQLVTAKQPLPEMLDPRQIGEFVLFLCS 272
+ PG +T + + + + + ++ PL ++ P I + VLFL S
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGI---PLKKLAKPSDIADAVLFLVS 240

Query: 273 SAARGITGASLAMDGAWT 290
A IT +L +DG T
Sbjct: 241 GQAGHITMHNLCVDGGAT 258


86Shewana3_3569Shewana3_3578N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3569-2152.004706MATE efflux family protein
Shewana3_3570-1131.620287LysR family transcriptional regulator
Shewana3_3571-2121.604025RND family efflux transporter MFP subunit
Shewana3_3572-2131.382200acriflavin resistance protein
Shewana3_3573-2131.492509hypothetical protein
Shewana3_3574-2131.833959FxsA cytoplasmic membrane protein
Shewana3_3575-2131.537427CutA1 divalent ion tolerance protein
Shewana3_3576-2122.274810thiol:disulfide interchange protein
Shewana3_3577-2102.287879sodium/hydrogen exchanger
Shewana3_3578-1132.361493galactokinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3569SECFTRNLCASE310.007 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 31.3 bits (71), Expect = 0.007
Identities = 22/114 (19%), Positives = 45/114 (39%), Gaps = 14/114 (12%)

Query: 169 MALAAIINLILDPLLIFGIGPFPRLEIQGAAIATLFSWLIALSLSGYLLIIKRNMLEWAA 228
AL A++ L+ D LL G+ +L+ +A L + + S++ +++
Sbjct: 178 FALGAVVALVHDVLLTVGLFAVLQLKFDLTTVAALLT-ITGYSINDTVVV---------- 226

Query: 229 FDIDRMRANWSKLAHIAQPAALMNLINP-LANAVIMAMLAHIDHSAVAAFGAGT 281
DR+R N K + + +N L+ V+ M + + +G
Sbjct: 227 --FDRLRENLIKYKTMPLRDVMNLSVNETLSRTVMTGMTTLLALVPMLIWGGDV 278


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3571RTXTOXIND431e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 42.9 bits (101), Expect = 1e-06
Identities = 43/274 (15%), Positives = 91/274 (33%), Gaps = 49/274 (17%)

Query: 25 SSVQASAIRPVKLFEVMQLEGGDFRTFPAR--VSANSRAELSFRISGELTDLALVEGQ-- 80
+V A R L V + DF + + ++ ++ E + + +L + + Q
Sbjct: 217 LTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLE 276

Query: 81 QIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTELLKRKLISQAEFDSAQAQLKS 140
QI L AK E++L+ F+ + + +
Sbjct: 277 QIESEILSAKE--------------EYQLVTQLFKNEI---------LDKLRQTTDNIGL 313

Query: 141 AKAALAAARDQLSYTKLIAPFSGTVAKRLVDNH-QIVQANQGILTL-QNNSLLDVSIQVP 198
LA ++ + + AP S V + V +V + ++ + + L+V+ V
Sbjct: 314 LTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQ 373

Query: 199 EAMAASLNNYIQQQNFTAKVRFSALAGMEF---DAKFKEYSTQVTPGTQ---AYEVVFSL 252
+ A ++ A + K K + + + V+ S+
Sbjct: 374 NKDIGFI-----NVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISI 428

Query: 253 PQP------KDIQLLPGMSAELTLALVKTPDQTA 280
+ K+I L GM+ A +KT ++
Sbjct: 429 EENCLSTGNKNIPLSSGMAVT---AEIKTGMRSV 459



Score = 28.6 bits (64), Expect = 0.036
Identities = 11/84 (13%), Positives = 30/84 (35%), Gaps = 5/84 (5%)

Query: 68 SGELTDLALVEGQQIRQGSLLAKLDDRDAYNNLMTREAEHELLAADFQRKTELLKRKLIS 127
+ + ++ + EG+ +R+G +L KL A + + ++ + R + L
Sbjct: 104 NSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTR-----YQILSR 158

Query: 128 QAEFDSAQAQLKSAKAALAAARDQ 151
E + + ++
Sbjct: 159 SIELNKLPELKLPDEPYFQNVSEE 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3572ACRIFLAVINRP492e-159 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 492 bits (1267), Expect = e-159
Identities = 207/1046 (19%), Positives = 441/1046 (42%), Gaps = 54/1046 (5%)

Query: 3 IAEYSIRHKVISWMFVLLLLVGGGVSFTGLGQLEFPEFTIKEALVITAYPGASPEQVEEE 62
+A + IR + +W+ ++L++ G ++ L ++P V YPGA + V++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VTLPLEDALQQLDAVKHVTSI-NSAGLSQIQIEIKETYDKTSLPQVWDEVRRKVNDTAGQ 121
VT +E + +D + +++S +SAG I + + T +V+ K+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQ---SGTDPDIAQVQVQNKLQLATPL 117

Query: 122 LPPGTSTPKVYDDFGD---VYGILFNLSGPDYSNRELSNYAD-YLRRELVLVPGVKKVSV 177
LP + + + F P + ++S+Y ++ L + GV V +
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 178 AGSVTEQVVIEISQQKLSALGLDQSYIYGLINNQNVVSNAGSLVVGDN------RIRIHP 231
G+ + I + L+ L + + QN AG L I
Sbjct: 178 FGA-QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 232 TGEFSSVQDLARLIVSPPGSTELIYLGDIAHIEKDYDETPDVLYHNRGEAALSLGISFSS 291
F + ++ ++ + ++ L D+A +E + + N G+ A LGI ++
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARIN-GKPAAGLGIKLAT 295

Query: 292 GVNVVEVGKSVSERLAELESQRPIGMNLDTVYNQSLAVDDTVNGFLINLLESIAIVIAVL 351
G N ++ K++ +LAEL+ P GM + Y+ + V +++ + L E+I +V V+
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVM 355

Query: 352 LLFMG-LRSGLLMGLILLLTILGTFIVMKVLGIELQLISLGALIIALGMLVDNAIVVTEG 410
LF+ +R+ L+ + + + +LGTF ++ G + +++ +++A+G+LVD+AIVV E
Sbjct: 356 YLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVEN 415

Query: 411 ILIGLRRGKTR-LEAAKQIVTQTQWPLLGATVIAIIAFAPIGLSQNAAGEFCRSLFQVLM 469
+ + K EA ++ ++Q Q L+G ++ F P+ + G R ++
Sbjct: 416 VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIV 475

Query: 470 ISLFISWITAITLTPFFCHLLFKDAPADD-EEQDPYKGWFFSLYRASLTFA-------LR 521
++ +S + A+ LTP C L K A+ E + + GWF + + S+ L
Sbjct: 476 SAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILG 535

Query: 522 FRIASILLVGAMLVTAVIGFGHIKNVFFPASNTPIFFVDIWMPEGTDIKGTERFTADIEQ 581
+L+ ++ V+ F + + F P + +F I +P G + T++ +
Sbjct: 536 STGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTD 595

Query: 582 LLLKQAEEQHSGLKHLTTVIG-------QGAQRFVLPYQPEKGYPAFAQLIIEMEDLASL 634
LK + ++ + TV G Q A + +P + +
Sbjct: 596 YYLKNEKA---NVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSA--EAVIHRA 650

Query: 635 KVYMSELERQLNQRFPQAQYRFKNMENGPSPAAKIEARFYGDDPEVLRALGAQAEAIFHA 694
K+ + ++ F + ++ + + +A
Sbjct: 651 KMELGKIRDGFVIPFNMP--AIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQH 708

Query: 695 EPSMDGVRHDWRNQVPLIRPQLQNAQARETGISKQDLDNALLINFSGKQIGLYRETSHLL 754
S+ VR + + ++ +A+ G+S D++ + G + + + +
Sbjct: 709 PASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVK 768

Query: 755 PIVARAPAEERLQADSLWKLQIWSSEHSTFVPATQVVSQFETQWENPLVKRRDRMRMLAV 814
+ +A A+ R+ + + KL + S + VP + + + +P ++R + + + +
Sbjct: 769 KLYVQADAKFRMLPEDVDKLYV-RSANGEMVPFSAFTT-SHWVYGSPRLERYNGLPSMEI 826

Query: 815 LADPKLGSD-ETADSVLRKVKDKVEAISIPAGYHLEWGGEYETAGEAQTAVFSSIPLGYL 873
+ G+ A +++ + K +PAG +W G + + + + ++
Sbjct: 827 QGEAAPGTSSGDAMALMENLASK-----LPAGIGYDWTGMSYQERLSGNQAPALVAISFV 881

Query: 874 AMFLITVFLFNSVRQPLVIWFTVPLALIGVSAGLLIFDAPFSFMALLGLLSLSGMVIKNG 933
+FL L+ S P+ + VPL ++GV +F+ ++GLL+ G+ KN
Sbjct: 882 VVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNA 941

Query: 934 IVLVDQIN-LELDEGKPAYIALVDSSVSRVRPVMMAAITTMLGMIPLIPDAFFGS----- 987
I++V+ L EGK A + + R+RP++M ++ +LG++PL GS
Sbjct: 942 ILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNA 1001

Query: 988 MAITIIFGLGFASLLTLIVLPVMYSL 1013
+ I ++ G+ A+LL + +PV + +
Sbjct: 1002 VGIGVMGGMVSATLLAIFFVPVFFVV 1027



Score = 74.1 bits (182), Expect = 2e-15
Identities = 44/219 (20%), Positives = 96/219 (43%), Gaps = 13/219 (5%)

Query: 813 AVLADPKLGSDETADSVLRKVKDKVEAI--SIPAGYHLEWGGEYETAGEAQTAVFSSIPL 870
A KL + A + +K K+ + P G ++ Y+T Q ++ +
Sbjct: 286 AAGLGIKLATGANALDTAKAIKAKLAELQPFFPQG--MKVLYPYDTTPFVQLSIHEVVKT 343

Query: 871 GYLAMFL--ITVFLF-NSVRQPLVIWFTVPLALIGVSAGLLIFDAPFSFMALLGLLSLSG 927
+ A+ L + ++LF ++R L+ VP+ L+G A L F + + + G++ G
Sbjct: 344 LFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIG 403

Query: 928 MVIKNGIVLVDQINLELDEGKPAYIALVDSSVSRVR-PVMMAAITTMLGMIPL-----IP 981
+++ + IV+V+ + + E K + S+S+++ ++ A+ IP+
Sbjct: 404 LLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGST 463

Query: 982 DAFFGSMAITIIFGLGFASLLTLIVLPVMYSLAFNIKPN 1020
A + +ITI+ + + L+ LI+ P + +
Sbjct: 464 GAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSA 502


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3573cloacin260.039 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 26.2 bits (57), Expect = 0.039
Identities = 16/70 (22%), Positives = 23/70 (32%), Gaps = 1/70 (1%)

Query: 40 AKNAAVKATTGVSGRCTPEKAAERTAKDAKDAVGDAKDKVTDSVSDKVDDIKPDDKLSD- 98
A A +S K E + A++ + D K+K D D P K +
Sbjct: 414 AAKEKSDADAALSSAMESRKKKEDKKRSAENNLNDEKNKPRKGFKDYGHDYHPAPKTENI 473

Query: 99 KAADALKQDK 108
K LK
Sbjct: 474 KGLGDLKPGI 483


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3577FLGHOOKAP1350.001 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 34.5 bits (79), Expect = 0.001
Identities = 26/140 (18%), Positives = 49/140 (35%), Gaps = 22/140 (15%)

Query: 412 GEILGIE---HKQELVDLHRANGRNVVQGDASDTDFWEKLDRAPNLELVLLAMPHHAGNL 468
+I+G+E ++ ANG ++VQG S + + + +A
Sbjct: 209 NQIVGVEVSVQDGGTYNITMANGYSLVQG--STARQLAAVPSSADPSRTTVAYVDGTAG- 265

Query: 469 FAVEQLKKLNYQGKLSAIV--------QYGDDAASLRSSGVHSVYNLYEA-------AGA 513
+E +KL G L I+ Q + L + + ++A AG
Sbjct: 266 -NIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGE 324

Query: 514 GFVDHVVHELLPDSETKADA 533
F +L +++ K D
Sbjct: 325 DFFAIGKPAVLQNTKNKGDV 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3578RTXTOXINA290.031 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 29.2 bits (65), Expect = 0.031
Identities = 11/41 (26%), Positives = 21/41 (51%), Gaps = 4/41 (9%)

Query: 124 LAAGLSSSGALVVAFGTAISDSSQLHLSPMAVAQLAQRGEY 164
A GLS+S A +A++ L +SP++ +A + +
Sbjct: 296 AAQGLSTSAAAAGLIASAVT----LAISPLSFLSIADKFKR 332


87Shewana3_3648Shewana3_3663N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3648115-1.456885rod shape-determining protein MreB
Shewana3_3649015-0.266709MSHA biogenesis protein MshQ
Shewana3_36500190.201222MSHA biogenesis protein MshP
Shewana3_3651018-0.150043methylation site containing protein
Shewana3_3652017-0.006030methylation site containing protein
Shewana3_3653-1170.666057methylation site containing protein
Shewana3_3655-1151.257935IS4 family transposase
Shewana3_3656-1170.636263methylation site containing protein
Shewana3_3657-2161.782818methylation site containing protein
Shewana3_3658-3151.829139hypothetical protein
Shewana3_3659-3151.976433type II secretion system protein
Shewana3_3660-2141.575872type II secretion system protein E
Shewana3_3661-2141.412502hypothetical protein
Shewana3_3662-2150.838940MSHA biogenesis protein MshM
Shewana3_3663-317-0.021113pilus (MSHA type) biogenesis protein MshL
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3648SHAPEPROTEIN5570.0 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 557 bits (1437), Expect = 0.0
Identities = 315/348 (90%), Positives = 333/348 (95%), Gaps = 1/348 (0%)

Query: 1 MFKKLRGIFSNDLSIDLGTANTLIYVRDEGIVLNEPSVVAIRGERSSSGQKSVAAVGTEA 60
M KK RG+FSNDLSIDLGTANTLIYV+ +GIVLNEPSVVAIR +R+ S KSVAAVG +A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGS-PKSVAAVGHDA 59

Query: 61 KQMLGRTPGNIQAIRPMKDGVIADFYVTEKMLQHFIKQVHNNSFFRPSPRVLVCVPVGAT 120
KQMLGRTPGNI AIRPMKDGVIADF+VTEKMLQHFIKQVH+NSF RPSPRVLVCVPVGAT
Sbjct: 60 KQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGAT 119

Query: 121 QVERRAIRESAMGAGAREVYLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAIISLN 180
QVERRAIRESA GAGAREV+LIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVA+ISLN
Sbjct: 120 QVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN 179

Query: 181 GVVYSSSVRIGGDKFDDAIINYVRRNYGSLIGEATAERIKHTIGTAYPGDEVLEIEVRGR 240
GVVYSSSVRIGGD+FD+AIINYVRRNYGSLIGEATAERIKH IG+AYPGDEV EIEVRGR
Sbjct: 180 GVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGR 239

Query: 241 NLAEGVPRSFTLNSNEILEALQEPLSGIVSAVMVALEQSPPELASDISERGMVLTGGGAL 300
NLAEGVPR FTLNSNEILEALQEPL+GIVSAVMVALEQ PPELASDISERGMVLTGGGAL
Sbjct: 240 NLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL 299

Query: 301 LRDLDRLLMQETGIPVMVADDPLTCVARGGGKALEMIDMHGGDLFSEE 348
LR+LDRLLM+ETGIPV+VA+DPLTCVARGGGKALEMIDMHGGDLFSEE
Sbjct: 300 LRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSEE 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3651BCTERIALGSPG343e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 33.7 bits (77), Expect = 3e-04
Identities = 12/24 (50%), Positives = 18/24 (75%)

Query: 8 RIARSSRGFTLVEMVTVILILGIL 31
R RGFTL+E++ VI+I+G+L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVL 25


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3652BCTERIALGSPH381e-05 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 37.6 bits (87), Expect = 1e-05
Identities = 15/58 (25%), Positives = 32/58 (55%), Gaps = 4/58 (6%)

Query: 21 KQQGFTLIELVVGMLVIAIAIVM-LSSMLFPQADRAAKTLHRVKSA-ELA--HSVMNE 74
+Q+GFTL+E+++ +L++ ++ M L + + D AA+TL R ++ +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTG 59


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3653BCTERIALGSPH409e-07 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 39.9 bits (93), Expect = 9e-07
Identities = 25/79 (31%), Positives = 39/79 (49%), Gaps = 1/79 (1%)

Query: 8 RQFGFTLVELVTTIILIGILSVTVLPRLFNQSSYSAFSLRNEFMAELRQVQQKALNNTDR 67
RQ GFTL+E++ ++L+G+ + VL SA F A+LR VQQ+ L +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQT-GQ 60

Query: 68 CYRVVVSATGYQVSQFASR 86
+ V V +Q +R
Sbjct: 61 FFGVSVHPDRWQFLVLEAR 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3656BCTERIALGSPG451e-08 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 44.9 bits (106), Expect = 1e-08
Identities = 14/31 (45%), Positives = 23/31 (74%)

Query: 2 MKRQQGFTLIELVVVIIILGILAVTAAPKFI 32
+Q+GFTL+E++VVI+I+G+LA P +
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLM 34


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3657BCTERIALGSPG438e-08 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 43.3 bits (102), Expect = 8e-08
Identities = 15/36 (41%), Positives = 27/36 (75%)

Query: 4 KQNGFSLIELVIVIVILGLLAATAIPRFLNVTDDAE 39
KQ GF+L+E+++VIVI+G+LA+ +P + + A+
Sbjct: 6 KQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKAD 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3659BCTERIALGSPF303e-102 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 303 bits (778), Expect = e-102
Identities = 119/407 (29%), Positives = 209/407 (51%), Gaps = 6/407 (1%)

Query: 1 MPVYQYRGRSGQGQAVTGQLDAASEGAAADMLLARGIIPLEVKV----AKETKSFTLAQL 56
M Y Y+ QG+ G +A S A +L RG++PL V +++ S L+
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 57 FKRKVGLDELQIFTRQMYSLTRSGIPILRAIAGLSETAHSQRMKDALNDISEQLTAGRPL 116
K ++ +L + TRQ+ +L + +P+ A+ +++ + + + + ++ G L
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 117 SSAMNQHPDVFDSLFVSMVHVGENTGKLEDAFIQLSGYIEREQETRRRIKAAMRYPIFVL 176
+ AM P F+ L+ +MV GE +G L+ +L+ Y E+ Q+ R RI+ AM YP VL
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPC-VL 179

Query: 177 IAIALAMV-ILNIMVIPKFAEMFARFGADLPWATKVLIGTSNLFVNYWPLMLVILLGTII 235
+A+A+V IL +V+PK E F LP +T+VL+G S+ + P ML+ LL +
Sbjct: 180 TVVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFM 239

Query: 236 GIRYWHHTEKGEKQWDKWKLHIPAVGSIIERSTLSRYCRSFSMMLSAGVPMTQALSLVAD 295
R EK + + LH+P +G I +RY R+ S++ ++ VP+ QA+ + D
Sbjct: 240 AFRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGD 299

Query: 296 AVDNAYMHDKIVGMRRGIESGESMLRVSNQSKLFTPLVLQMVAVGEETGQIDQLLNDAAD 355
+ N Y ++ + G S+ + Q+ LF P++ M+A GE +G++D +L AAD
Sbjct: 300 VMSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAAD 359

Query: 356 FYEGEVDYDLKNLTAKLEPILIGIVAAIVLVLALGIYLPMWDMLNVV 402
+ E + EP+L+ +AA+VL + L I P+ + ++
Sbjct: 360 NQDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLM 406


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3661IGASERPTASE401e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 40.4 bits (94), Expect = 1e-05
Identities = 38/187 (20%), Positives = 64/187 (34%), Gaps = 13/187 (6%)

Query: 70 NSVNPVASSQTQPDNASPQIDTSPLETETTKSTYTTIPTEPTAEMVQVSPSSTEVPAKDM 129
N P + + T D P +TS + + T E + + +T P +
Sbjct: 1154 NIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNS 1213

Query: 130 SASAESAPSVAQSARLNAAQVEP--TSEPRAEPVATNTSTDTSNNAALTQESAQTQAESQ 187
+S + +S R VEP TS VA T T+ NA L+ A+ Q +
Sbjct: 1214 ESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVAL 1273

Query: 188 QVAVKANQADVNANQSEVKIIQTDPKASEPVVPAATQASRQTS---PQASSPASAQAQST 244
V +Q + ++ + + V + T ++ S + S S Q Q
Sbjct: 1274 NVGKAVSQ--------HISQLEMNNEGQYNVWVSNTSMNKNYSSSQYRRFSSKSTQTQLG 1325

Query: 245 GQMAIRE 251
I
Sbjct: 1326 WDQTISN 1332



Score = 37.4 bits (86), Expect = 1e-04
Identities = 31/169 (18%), Positives = 57/169 (33%), Gaps = 15/169 (8%)

Query: 73 NPVASSQTQPDNASPQIDTSPLETETTKSTYTTIPTEPTAEMVQ-----VSPSSTEVPAK 127
NP + Q +DT+ + T E+ + V P + P++
Sbjct: 982 NPEVEKRNQT------VDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSE 1035

Query: 128 DMSASAESAPSVAQSARLN---AAQVEPTSEPRAEPVATNTSTDTSNNAALTQESAQTQA 184
AE++ +++ N A + + A+ +N +T N S +
Sbjct: 1036 TTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKET 1095

Query: 185 ESQQVAVKANQADVNANQSEVKIIQTDPKASEPVVPAATQASRQTSPQA 233
++ + A + E + Q PK + V P Q S PQA
Sbjct: 1096 QTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQ-SETVQPQA 1143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3663BCTERIALGSPD1877e-54 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 187 bits (476), Expect = 7e-54
Identities = 78/317 (24%), Positives = 144/317 (45%), Gaps = 28/317 (8%)

Query: 237 ELKETLSAIIGDTGGGRQVVVT--PQAGLVTIRAYPNELRQVRAFLNSAESHLQRQVILE 294
++ A + +++ Q + + A P+ + + + + + QV++E
Sbjct: 292 TMQSEKQAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIR-RPQVLVE 350

Query: 295 AKILEVTLSDGYQQGIQWDNVLGHV---GNTNINFGTSAGAGLS----DKITASLGGVTS 347
A I EV +DG GIQW N + N+ + T+ +++SL S
Sbjct: 351 AIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALS 410

Query: 348 ------LSIKGSDFNTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSST 401
++ +++ L + D+L++P + +N +A VG + +T S
Sbjct: 411 SFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLT--GSQ 468

Query: 402 TVAGTTPVTTPQVELTPFFSGIALDVTPQIDKDGNVLLHVHPSVIDVKEQTKDIKVSSES 461
T +G T + + GI L V PQI++ +VLL + V V + SS S
Sbjct: 469 TTSGDNIFNTVERKTV----GIKLKVKPQINEGDSVLLEIEQEVSSVADAA-----SSTS 519

Query: 462 LELPLAQSEIRESDTVIRAASGDVVVIGGLMKSENTEVVSQVPLLGDIPLVGELFKNRSK 521
+L + R + + SG+ VV+GGL+ ++ +VPLLGDIP++G LF++ SK
Sbjct: 520 SDLGATFN-TRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSK 578

Query: 522 QKKKTELIIMLKPTVVG 538
+ K L++ ++PTV+
Sbjct: 579 KVSKRNLMLFIRPTVIR 595


88Shewana3_3705Shewana3_3711N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_37050172.201824C factor cell-cell signaling protein
Shewana3_3706-1172.583041hypothetical protein
Shewana3_37070182.398853response regulator receiver protein
Shewana3_3708-1182.142040ATPase domain-containing protein
Shewana3_37090192.140889dTDP-4-dehydrorhamnose reductase
Shewana3_3710-1121.749516hypothetical protein
Shewana3_3711-1131.464177hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3705DHBDHDRGNASE451e-07 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 44.7 bits (105), Expect = 1e-07
Identities = 36/197 (18%), Positives = 73/197 (37%), Gaps = 33/197 (16%)

Query: 55 LEEGVKQLSQNIPQLDWLINCIGMLHTE--DKGPEKSVQAVDGDFFLHNIKLNTLPSMVL 112
++E ++ + + +D L+N G+L ++ +A +N+
Sbjct: 72 IDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEAT--------FSVNSTGVFNA 123

Query: 113 AKHFESALKRSASARFAVVSAKVGSISDNRLGGWYSYRASKAALNMFLKTLSIEWQRTMK 172
++ + S V + + + +Y +SKAA MF K L +E
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMA---AYASSKAAAVMFTKCLGLELAEYNI 180

Query: 173 HCVVLSLHPGTTDTRLSKP------------------FQQNVPKQKLFTPEYVAQCLVSI 214
C ++S PG+T+T + F+ +P +KL P +A ++ +
Sbjct: 181 RCNIVS--PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFL 238

Query: 215 IANATPAQTGSFLAYDG 231
++ T L DG
Sbjct: 239 VSGQAGHITMHNLCVDG 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3707HTHFIS872e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 2e-22
Identities = 26/110 (23%), Positives = 57/110 (51%)

Query: 3 RLLIIEDDQALAGVLARRLTRHGFECRLSHDASNALLVAREFCPSHILLDMKLAEANGLS 62
+L+ +DD A+ VL + L+R G++ R++ +A+ ++ D+ + + N
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 LIVPLRNLLPKVIMVLLTGYASIATAVEAIRLGADNYLAKPVDTQTLLAA 112
L+ ++ P + +++++ + TA++A GA +YL KP D L+
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGI 114



Score = 48.3 bits (115), Expect = 3e-09
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 2/58 (3%)

Query: 116 NSQASALPEDEIDDSPLSPKRLEWEHIQQVLNANQGNVSATARQLGMHRRTLQRKLLK 173
S ALP + D L +E+ I L A +GN A LG++R TL++K+ +
Sbjct: 417 ASFGDALPPSGLYDRVL--AEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3709NUCEPIMERASE662e-14 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 66.0 bits (161), Expect = 2e-14
Identities = 40/160 (25%), Positives = 63/160 (39%), Gaps = 24/160 (15%)

Query: 3 NIMVTGATGLLGRAVVKQLTAAGHRVIATGF---------SRAEAGIHLL---------- 43
+VTGA G +G V K+L AGH+V+ G S +A + LL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVV--GIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59

Query: 44 DLTQAAEVEAFIAREQPEVIVHCAAERRPDVSERSPEHALALNLSASQTLAEVAKTHQ-A 102
DL + A E + S +P NL+ + E + ++
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 103 WLLYISTDYVF-DGTTPPYAE-DAVPNPVNFYGESKLQGE 140
LLY S+ V+ P++ D+V +PV+ Y +K E
Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANE 159


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3711SECETRNLCASE280.005 Bacterial translocase SecE signature.
		>SECETRNLCASE#Bacterial translocase SecE signature.

Length = 127

Score = 28.3 bits (63), Expect = 0.005
Identities = 16/56 (28%), Positives = 26/56 (46%), Gaps = 5/56 (8%)

Query: 3 KSAIVSILLCAAVLLGAMYFGGWTKHPYVQELTTEIRKASAVIEPKKPSAEAMQDT 58
++ V IL+ AA + + G + +E TE+RK VI P + E + T
Sbjct: 44 RALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRK---VIWPTRQ--ETLHTT 94


89Shewana3_3816Shewana3_3820N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_38161181.538727hypothetical protein
Shewana3_38170161.326875putative signal transduction histidine kinase
Shewana3_38180162.059450two component LuxR family transcriptional
Shewana3_3819-1162.194444hypothetical protein
Shewana3_3820-1162.732057Mg chelatase subunit ChlI
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3816cloacin280.029 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 28.1 bits (62), Expect = 0.029
Identities = 23/91 (25%), Positives = 38/91 (41%), Gaps = 11/91 (12%)

Query: 16 PVFAASNSSINIAEVDKAASTLNIEKLQQLSTTTQDYEAAYANYRLAIS---------AN 66
V+ + + ++ +V + N + QQ T EAA NY A + A
Sbjct: 282 AVYVSVSDVLSPDQVKQRQDEEN--RRQQEWDATHPVEAAERNYERARAELNQANEDVAR 339

Query: 67 VMGQKTLAEQALTSAQTSLEAINNTQADAES 97
++ A Q S ++ L+A N T ADA +
Sbjct: 340 NQERQAKAVQVYNSRKSELDAANKTLADAIA 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3817PF06580310.006 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 31.0 bits (70), Expect = 0.006
Identities = 59/352 (16%), Positives = 114/352 (32%), Gaps = 65/352 (18%)

Query: 10 QKIAWVYLINLAFYLIPLFVTPYPSWQIGLILAVLIPFIYSYFWAYRCHTRVAYRPISVM 69
Q I W F L+ +P I I L+ + + AYR R +
Sbjct: 16 QGIGWGVYTLTGFGFASLYGSPKLHSMIFNIAISLMGLVLT--HAYR---SFIKRQGWLK 70

Query: 70 LLLAIAITPINPGSISLFTFASFFIGFFYPLRVSLLSWFGILALLFLLNHFSQFNNLYFP 129
L + I + P + LL++ + F L ++ F
Sbjct: 71 LNMGQIILRVLPACV----VIGMVWFVANTSIWRLLAFINTKPVAFTL---PLALSIIFN 123

Query: 130 LYGSALVFGVGMLGIAEQKRYQHRLKERQSAQEISTLATMVERERIARDLHDIMGH---- 185
+ ++ + G K Y+ E + +A+M + ++ I H
Sbjct: 124 VVVVTFMWSLLYFGWHFFKNYKQA--EIDQWK----MASMAQEAQLMALKAQINPHFMFN 177

Query: 186 SLSSIALKAELAEKLLAKQEYQLATTQLQELGQIARESLSQIRH----------TVSDYK 235
+L++I L ++ A L L ++ R SL V Y
Sbjct: 178 ALNNIRA--------LILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSY- 228

Query: 236 HKGLASCVTQLCHSLR-DKGIAVELLG-ELPKLSARAESQLGLVLTELVNNILRH----- 288
L Q L+ + I ++ ++P + ++ LV N ++H
Sbjct: 229 ---LQLASIQFEDRLQFENQINPAIMDVQVPPM----------LVQTLVENGIKHGIAQL 275

Query: 289 SSASQCRIQFREQADSLIVDVQDN----APTSPIIEGNGLTGIRERLTSLGG 336
+ ++ + ++ ++V++ + G GL +RERL L G
Sbjct: 276 PQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQNVRERLQMLYG 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3818HTHFIS808e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 79.9 bits (197), Expect = 8e-20
Identities = 30/117 (25%), Positives = 51/117 (43%), Gaps = 1/117 (0%)

Query: 2 HILLAEDQAMVRGALAALLTLAGGFTITQASNGDEALNLLKQQHFDLLLTDIEMPGRTGL 61
IL+A+D A +R L L+ AG + + SN + DL++TD+ MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRAG-YDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 ELATWLKEQHSHTKVVVITTFGRAGYIKRAIEAGVGGFLLKDAPSETLVHAIQQVIA 118
+L +K+ V+V++ +A E G +L K L+ I + +A
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3820HTHFIS395e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 38.7 bits (90), Expect = 5e-05
Identities = 33/149 (22%), Positives = 57/149 (38%), Gaps = 17/149 (11%)

Query: 202 QAKQALEIAAAGNHNLLMLGPPGTGKTMLASRIMALLPILNYE-EALEVAAIHSVAGINI 260
+ + L + L++ G GTGK ++A + N A+ +AAI
Sbjct: 148 EIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAI-------- 199

Query: 261 KPQDFLKRPFRAPHHTSSSISLVGGGSIPKPGEISLAHRGVLFLDEVAEFPRKVLDCLRE 320
P+D ++ H + + G G A G LFLDE+ + P L
Sbjct: 200 -PRDLIESELFG--HEKGAFT---GAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLR 253

Query: 321 PMETGEVVISRAAAKLTFLSRFQLIAAMN 349
++ GE + + S +++AA N
Sbjct: 254 VLQQGE--YTTVGGRTPIRSDVRIVAATN 280


90Shewana3_3846Shewana3_3852N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3846442-9.763248hypothetical protein
Shewana3_3847347-10.861050hypothetical protein
Shewana3_3848347-11.364869RND family efflux transporter MFP subunit
Shewana3_3849344-10.501096acriflavin resistance protein
Shewana3_3850339-9.602265TetR family transcriptional regulator
Shewana3_3851233-8.094191diguanylate cyclase
Shewana3_3852430-6.993745CRISPR-associated Csy4 family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3846TCRTETB290.029 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 28.7 bits (64), Expect = 0.029
Identities = 17/125 (13%), Positives = 46/125 (36%), Gaps = 12/125 (9%)

Query: 107 YWPALITLFLAMLDMVVRLNLHKNALWFQAEFFKHRQDIDLSHWSLA----LPLLLFIQT 162
+W L+ + + + V L + + + + D+ L + +LF +
Sbjct: 167 HWSYLLLIPMITIITVPFL------MKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTS 220

Query: 163 ATY-FFALWRLFQRHKLRQQQRNEAPQQTLKLIRFR-WLFGLTLAMLFNWLLRVFVVVLP 220
+ F + L ++ ++ P L + ++ G+ + + FV ++P
Sbjct: 221 YSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVP 280

Query: 221 FYLGD 225
+ + D
Sbjct: 281 YMMKD 285


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3848RTXTOXIND476e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.1 bits (112), Expect = 6e-08
Identities = 19/117 (16%), Positives = 47/117 (40%), Gaps = 15/117 (12%)

Query: 59 VSSVKEAVLAFEVPGKVIKLHVKEGDFVKQDTVLAEIDDRDYQAQLDSAKSDLIVAKSDF 118
S + + E V ++ VKEG+ V++ VL ++ +A +S L+ A+ +
Sbjct: 92 HSGRSKEIKPIE-NSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ 150

Query: 119 QRYE---KALKADAVTPQAF-----------QQAKRNLEVAQAAFNQADKALTETKL 161
RY+ ++++ + + ++ R + + F+ + +L
Sbjct: 151 TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKEL 207



Score = 42.5 bits (100), Expect = 2e-06
Identities = 37/190 (19%), Positives = 74/190 (38%), Gaps = 29/190 (15%)

Query: 100 YQAQLDSAKSDLIVAKSDFQRYEKALKADAVTPQAFQQAKRNLEVAQAAFNQADKALTET 159
Y++QL+ +S+++ AK ++Q + K + + Q N+ + + ++ +
Sbjct: 271 YKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLR--QTTDNIGLLTLELAKNEERQQAS 328

Query: 160 KLIAPFAGRVVTREI-DLFATVHAKQPIMQL-HSESAYEMVVSVPESDWAQGERVNSAAD 217
+ AP + +V ++ V + +M + + E+ V D V A
Sbjct: 329 VIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGF-INVGQNAI 387

Query: 218 IKLDNQLFVTLTAFPDKRF---EGKITEFSGQADAAT--RT---YKVKVAFSVP------ 263
IK++ AFP R+ GK+ + DA R + V ++
Sbjct: 388 IKVE--------AFPYTRYGYLVGKVKNIN--LDAIEDQRLGLVFNVIISIEENCLSTGN 437

Query: 264 DKTPISSGMT 273
P+SSGM
Sbjct: 438 KNIPLSSGMA 447


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3849ACRIFLAVINRP459e-147 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 459 bits (1182), Expect = e-147
Identities = 220/1050 (20%), Positives = 445/1050 (42%), Gaps = 67/1050 (6%)

Query: 3 IASVAINNRVVTLVLTVVMLIAGLYIFNGMSRLEDPEFTIKDALIITPYNGASALEVEQE 62
+A+ I + VL +++++AG + + P + Y GA A V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VTELLEKTVQQLGELDKVTSKSER-GLSTITVTIKEQYNKETLPQVWNKLRQKIDDVKYY 121
VT+++E+ + + L ++S S+ G TIT+T + + + +++ K+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPD---IAQVQVQNKLQLATPL 117

Query: 122 LPPGA-GPSLVIDDYGDVYSIFMVV----SGDGYSFKELKTYVND-LQQQLLLVNGVGKI 175
LP + ++ S MV G + ++ YV ++ L +NGVG +
Sbjct: 118 LPQEVQQQGISVEKSSS--SYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDV 175

Query: 176 TTFGEKSEAIYIEFNRSRMAQLGISPEIVAAQLNGKGLVVDAGRAHVGSS------SIAV 229
FG + A+ I + + + ++P V QL + + AG+ + + ++
Sbjct: 176 QLFGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASI 234

Query: 230 STTGGFTKVSDFEKLLI--THDTKQFYLSDIAKVSRGYVSPSTQLINFDGKAGIGLGIST 287
F +F K+ + D L D+A+V G + +GK GLGI
Sbjct: 235 IAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELG-GENYNVIARINGKPAAGLGIKL 293

Query: 288 VSGGNTVDMGEAVLAKLSELESQRPAGIEFGYVSLQSEGVKEAISGFTSSLAEAVIIVIV 347
+G N +D +A+ AKL+EL+ P G++ Y + V+ +I +L EA+++V +
Sbjct: 294 ATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFL 353

Query: 348 VLLFFMG-LRSGLLIGFVLILTIAGSFIFLAPMGVALERISLGALIIALGMLVDNAIVVV 406
V+ F+ +R+ L+ + + + G+F LA G ++ +++ +++A+G+LVD+AIVVV
Sbjct: 354 VMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVV 413

Query: 407 DGILIRMQK-GESAESAAPRVVNQSAWPLLGATLIAILAFAAIGTSNDATGEYCRSLFQV 465
+ + M + + A + ++Q L+G ++ F + +TG R
Sbjct: 414 ENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSIT 473

Query: 466 VMVSLLLSWVTAVTITPLLCVMYLKAPKSTDKTSPYQGTFYT-----------KYRGLLA 514
++ ++ LS + A+ +TP LC LK + + G F+ Y +
Sbjct: 474 IVSAMALSVLVALILTPALCATLLKPVSAEHHENK--GGFFGWFNTTFDHSVNHYTNSVG 531

Query: 515 SSIRHRYLSSASIIGIFALSLWGFSFVQQNFFPSSTRAQFMVDFWLPQGTHIEETQKHAE 574
+ I A + F + +F P + F+ LP G E TQK +
Sbjct: 532 KILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLD 591

Query: 575 SVENYLGNL--ANVEHVTTTIGEGALRFLLTYQPQQSNSSYAQF-LVDVDDYTVIKTLIP 631
V +Y ANVE V T G ++ Q N+ A L ++ +
Sbjct: 592 QVTDYYLKNEKANVESVFTVNG-------FSFSGQAQNAGMAFVSLKPWEERNGDENSAE 644

Query: 632 KIEVELLQKY---PDALVYASP----FELGTGTAGKIQ-ARISGPDTDVLRETSDKVLEV 683
+ + D V ELGT T + +G D L + +++L +
Sbjct: 645 AVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGM 704

Query: 684 FSKE-SNTKGVRTDWRNKQLYIEAVLAEEQANINGINRGMVAEAIKESFEGVTTGVYREN 742
++ ++ VR + + + +E+A G++ + + I + G + +
Sbjct: 705 AAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDR 764

Query: 743 DLLLPIIIRANENERSDITNIENVQIWSPNAQKMIPLRQVVQSFETKFEDGLILRRNRER 802
+ + ++A+ R +++ + + S N + M+P + + + R N
Sbjct: 765 GRVKKLYVQADAKFRMLPEDVDKLYVRSANGE-MVPFSAFT-TSHWVYGSPRLERYNGLP 822

Query: 803 TITIFADPVTG-TASELLATLKPQVEAIKIPPGYTLEWGGEYEDSSKAEKGLASSIPIFI 861
++ I + G ++ + +A ++ K+P G +W G + + + I
Sbjct: 823 SMEIQGEAAPGTSSGDAMALMENLAS--KLPAGIGYDWTGMSYQERLSGNQAPALVAISF 880

Query: 862 LSMILITIVIFNSLKQTLVIWLCVPLALIGVTAGMLATNQPFGFMALLGFLSLIGMLIKN 921
+ + L ++ S + + L VPL ++GV NQ ++G L+ IG+ KN
Sbjct: 881 VVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKN 940

Query: 922 AIVLVDEIN-LEQSQGKSLINSILDSGVSRLRPVAMAALTTALGMIPLIFD-----AFFV 975
AI++V+ L + +GK ++ + L + RLRP+ M +L LG++PL
Sbjct: 941 AILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQN 1000

Query: 976 SMAVTIISGLMFATALTMIVLPIVYALIFK 1005
++ + ++ G++ AT L + +P+ + +I +
Sbjct: 1001 AVGIGVMGGMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3850HTHTETR654e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 64.6 bits (157), Expect = 4e-15
Identities = 30/184 (16%), Positives = 72/184 (39%), Gaps = 9/184 (4%)

Query: 19 DEKRNELLCTAINLLVTDGYAGLSMRNLASKAQVTTGAITYYFSNKSELMKGVSDELFNR 78
E R +L A+ L G + S+ +A A VT GAI ++F +KS+L + + +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 79 FSLLLA-------KNTEIDIKQAVEEWINWTYSDGGE-IWKAFLQVQFYAAEDKTFIDYA 130
L + +++ + + T ++ + + + + + A
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 131 QKRYDI-FISQLKHFIESGQKSKQIRNDIPAEILAEQLSAMGDGMMIMQSIYPERLSIEK 189
Q+ + +++ ++ ++K + D+ A + G+M P+ ++K
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKK 189

Query: 190 IAQF 193
A+
Sbjct: 190 EARD 193


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3852BINARYTOXINB280.021 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 28.5 bits (63), Expect = 0.021
Identities = 22/86 (25%), Positives = 35/86 (40%), Gaps = 7/86 (8%)

Query: 118 RLKRLEKRALVRGETFNPIKNIQPREFYTFHRIAISSGSNKQDYLLHIQKMVAK----EQ 173
+ LEK +R +T NI F R+ + +GSN + L IQ+ A+ +
Sbjct: 467 QFLELEKTKQLRLDTDQVYGNIATYNFEN-GRVRVDTGSNWSEVLPQIQETTARIIFNGK 525

Query: 174 TEPLFSSYGVASNLQ--LNGTVPELS 197
L A N L T P+++
Sbjct: 526 DLNLVERRIAAVNPSDPLETTKPDMT 551


91Shewana3_3891Shewana3_3899N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_3891-117-2.420144DNA adenine methylase
Shewana3_3892-117-2.702703sporulation domain-containing protein
Shewana3_3893017-4.0475833-dehydroquinate synthase
Shewana3_3894117-4.475171shikimate kinase I
Shewana3_3895015-2.667186type IV pilus secretin PilQ
Shewana3_3896-115-1.157719methyl-accepting chemotaxis sensory transducer
Shewana3_3897017-0.367799pilus assembly protein, PilO
Shewana3_3898-118-0.107426fimbrial assembly family protein
Shewana3_3899-2180.513961type IV pilus assembly protein PilM
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3891TYPE3IMSPROT346e-04 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 33.6 bits (77), Expect = 6e-04
Identities = 20/90 (22%), Positives = 31/90 (34%), Gaps = 17/90 (18%)

Query: 164 IGYEKAFEQIRTGDVIYCDPP-------YAPLSTTASFTTYVGAGFSLDDQALLARYSRH 216
I E ++ V+ +P Y T T+ D Q R
Sbjct: 245 IQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYT----DAQVQ---TVRK 297

Query: 217 MALEQRIPVVISNHDIPLTRELYRGAHLAK 246
+A E+ +P++ IPL R LY A +
Sbjct: 298 IAEEEGVPIL---QRIPLARALYWDALVDH 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3892PF05272300.027 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.027
Identities = 16/65 (24%), Positives = 24/65 (36%)

Query: 14 ALVERLHHVASYSDQLLVLVGAHGSGKTTLLTALATDFDESNAALVICPMHADNAEIRRK 73
V R+ D +VL G G GK+TL+ L S+ I +I
Sbjct: 583 GHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGI 642

Query: 74 ILVQL 78
+ +L
Sbjct: 643 VAYEL 647


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3894PF05272310.002 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 31.2 bits (70), Expect = 0.002
Identities = 16/64 (25%), Positives = 25/64 (39%), Gaps = 8/64 (12%)

Query: 9 LVGPMGAGKSTIGRHLAQML-----HLEFHDSDQEIEQRTGADIAWVFDVEGEEGFRRRE 63
L G G GKST+ L + H + EQ G +++ FRR +
Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAG---IVAYELSEMTAFRRAD 657

Query: 64 AQVI 67
A+ +
Sbjct: 658 AEAV 661


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3895BCTERIALGSPD2491e-75 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 249 bits (638), Expect = 1e-75
Identities = 99/411 (24%), Positives = 189/411 (45%), Gaps = 38/411 (9%)

Query: 306 GDITLRLDDVPWDQALDLILQTKGLDKRIEGNILMVAPSEELAIRESQNLKNKQEVKELA 365
GD ++ + W A D++ L+K + L + + E N
Sbjct: 190 GDRSVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSR 249

Query: 366 PLYSEYLQ----------------INYAKATDIAELLKGADSSLLSPRG----------- 398
++ + YAKA+D+ E+L G S++ S +
Sbjct: 250 QRIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKN 309

Query: 399 -SVAVDERTNTVLVKDTAEIIENIHRLVEVLDIPIRQVLIESRMVTVKDDVSEDLGIRWG 457
+ +TN ++V +++ ++ R++ LDI QVL+E+ + V+D +LGI+W
Sbjct: 310 IIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWA 369

Query: 458 VTDQQGSKGTSGTLEGAGSIANGVVPSLDNRLNVNLPAAVTNPTSIAFHVAKLADGTILD 517
+ ++ T+ L + +IA + D ++ +L +A+++ IA +
Sbjct: 370 NKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGN----WA 425

Query: 518 LELSALEQENKGEIIASPRITTSNQKAAYIEQGVEIPYV-----QSTSSGATSVTFKKAV 572
+ L+AL K +I+A+P I T + A G E+P + S + +V K
Sbjct: 426 MLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVG 485

Query: 573 LSLRVTPQITPDNRVILDLEITQDSQGKT-VDTPTGPAVAIDTQRIGTQVLVDNGETIVL 631
+ L+V PQI + V+L++E S T + +T+ + VLV +GET+V+
Sbjct: 486 IKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGSGETVVV 545

Query: 632 GGIYQQNLISRVSKVPILGDIPLVGFLFRNTTDKNERQELLIFVTPKIVNE 682
GG+ +++ KVP+LGDIP++G LFR+T+ K ++ L++F+ P ++ +
Sbjct: 546 GGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRD 596



Score = 46.8 bits (111), Expect = 2e-07
Identities = 33/175 (18%), Positives = 75/175 (42%), Gaps = 14/175 (8%)

Query: 275 SLNFQNISVRTVLQIIADYNNFNLVTSDTVEGDITLR-LDDVPWDQALDLILQTKGLDKR 333
S +F+ ++ + ++ N ++ +V G IT+R D + +Q L
Sbjct: 31 SASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVL----D 86

Query: 334 IEGNILMVAPSEELAIRESQNLKNKQEVK--ELAP-----LYSEYLQINYAKATDIAELL 386
+ G ++ + L + S++ K + AP + + + + A D+A LL
Sbjct: 87 VYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVVPLTNVAARDLAPLL 146

Query: 387 KGADSSLLSPRGSVAVDERTNTVLVKDTAEIIENIHRLVEVLDIPIRQVLIESRM 441
+ + + + GSV E +N +L+ A +I+ + +VE +D + ++ +
Sbjct: 147 RQLNDN--AGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPL 199


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_3899SHAPEPROTEIN431e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 42.8 bits (101), Expect = 1e-06
Identities = 32/156 (20%), Positives = 57/156 (36%), Gaps = 34/156 (21%)

Query: 199 VDIGANMTTFCVVESGETTFIREQAFGGELFTQSILSFYGMSY------EQAEKAKIE-- 250
VDIG T V+ + GG+ F ++I+++ +Y AE+ K E
Sbjct: 164 VDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIG 223

Query: 251 -------------------GDLPRNY------MFEVLSPFQTQLLQQIKRTLQIYCTSSG 285
+PR + + E L T ++ + L+
Sbjct: 224 SAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELA 283

Query: 286 KDKVDY-LVLCGGTSKLEGMANLLTNELGVHTIIAD 320
D + +VL GG + L + LL E G+ ++A+
Sbjct: 284 SDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAE 319


92Shewana3_4011Shewana3_4018N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Shewana3_4011-2122.107791hypothetical protein
Shewana3_4012-1122.173038sulfatase
Shewana3_4013-1122.157409RNA-binding S1 domain-containing protein
Shewana3_40140142.003898transcription elongation factor GreB
Shewana3_40150141.939330response regulator receiver modulated
Shewana3_40160131.628563putative PAS/PAC sensor protein
Shewana3_40170171.006482osmolarity response regulator
Shewana3_4018-1140.652207osmolarity sensor protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4011IGASERPTASE300.005 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.4 bits (68), Expect = 0.005
Identities = 18/132 (13%), Positives = 41/132 (31%), Gaps = 3/132 (2%)

Query: 36 QAATKGHEERAFNPQNERTADQTQQQARLLEQNQQQVQDKQQQQQSSQQQSQQQQEKQAP 95
+ A + N Q A Q + E + ++ ++ + + + ++ ++ P
Sbjct: 1067 EVAKEAKSNVKANTQTNEVA---QSGSETKETQTTETKETATVEKEEKAKVETEKTQEVP 1123

Query: 96 IVAADRALPKTLKVPVRGPAALQRKDIRLKVGQNTPRPANTTANTTTATTRQPMQGESPQ 155
V + + + V+ A R++ + NTTA+T E P
Sbjct: 1124 KVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPV 1183

Query: 156 FYQQVGQRIGQY 167

Sbjct: 1184 TESTTVNTGNSV 1195


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4015HTHFIS785e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 77.6 bits (191), Expect = 5e-18
Identities = 26/118 (22%), Positives = 53/118 (44%), Gaps = 3/118 (2%)

Query: 14 TKGKILIVDDQPLNIKILHQLFH-EEYEMFMATSGEQALQVCQKMQPDLVLLDIEMPEMT 72
T IL+ DD +L+Q Y++ + ++ + DLV+ D+ MP+
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 73 GYEVCQRLKADPATANIGVIFITAHFDEMEEVKGFQLGAVDFIHKPINPIITSARVKN 130
+++ R+K A ++ V+ ++A M +K + GA D++ KP + +
Sbjct: 62 AFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4016HTHFIS734e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 72.9 bits (179), Expect = 4e-15
Identities = 35/156 (22%), Positives = 68/156 (43%), Gaps = 7/156 (4%)

Query: 1287 TLLVVEDNQLNREVIDELLTYEGATVVLAEGGIEGVTQVLDSGDMFDAVIMDMQMPDIDG 1346
T+LV +D+ R V+++ L+ G V + + + D V+ D+ MPD +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWI--AAGDGDLVVTDVVMPDENA 62

Query: 1347 LEATRRIRADGRFDQLPILAMTANASQADKQECLEAGMNAHVSKPIDMQQLLPNILRLVG 1406
+ RI+ LP+L M+A + + E G ++ KP D+ +L+ I R +
Sbjct: 63 FDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 1407 RDAAQFAEPESMHLDAQHNLEGET--LLDDIRLILR 1440
+ ++ E L G + + + R++ R
Sbjct: 121 EPKRRPSKLED-DSQDGMPLVGRSAAMQEIYRVLAR 155



Score = 61.8 bits (150), Expect = 1e-11
Identities = 25/93 (26%), Positives = 39/93 (41%), Gaps = 8/93 (8%)

Query: 1138 LSGYRILVVDDNQITTEILSKILSDYGCVVETASGGYQAIEKVKQATANAQQFDVVLMDW 1197
++G ILV DD+ +L++ LS G V S + A D+V+ D
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIA-----AGDGDLVVTDV 55

Query: 1198 RMPDIDGLQTAEMLKNAGTGSYTPLVVMLTAYG 1230
MPD + +K A P++VM +A
Sbjct: 56 VMPDENAFDLLPRIKKA--RPDLPVLVM-SAQN 85


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4017HTHFIS1001e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 100 bits (251), Expect = 1e-26
Identities = 41/133 (30%), Positives = 73/133 (54%), Gaps = 4/133 (3%)

Query: 6 SKILVVDDDMRLRALLERYLMEQGYQVRSAANAEQMDRLLERENFHLIVLDLMLPGEDGL 65
+ ILV DDD +R +L + L GY VR +NA + R + + L+V D+++P E+
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 SICRRLRQQGSTIPIVMLTAKGDEVDRIIGLELGADDYLPKPFNPRELLARIKAVMRRQI 125
+ R+++ +P+++++A+ + I E GA DYLPKPF+ EL+ I R +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGII----GRAL 119

Query: 126 QDVPGAPAQQEAE 138
+ P++ E +
Sbjct: 120 AEPKRRPSKLEDD 132


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Shewana3_4018PF06580484e-08 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 47.6 bits (113), Expect = 4e-08
Identities = 19/120 (15%), Positives = 41/120 (34%), Gaps = 24/120 (20%)

Query: 325 DCPEALFQGLAIKRVLSNLVENAFRYG------SGWVRISSQFDGKRIGFSVEDNGPGID 378
A+ ++ LVEN ++G G + + D + VE+ G
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLAL 304

Query: 379 ESQIPKLFQPFTQGDIARGSVGSGLGLA-IIKRIIDRHQGQVTLS-NRTEGGLKAQVWLP 436
++ +G GL + +R+ + + + + +G + A V +P
Sbjct: 305 KNTKE----------------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348



 
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