PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
Home | Help | Analyzed genomes
 
A) Input parameters
GenomeNC_010170.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_010170 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1Bpet0041Bpet0052Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0041-1143.529717putative amino acid aldolase or racemase
Bpet0042-2142.575991acetylornithine transaminase protein
Bpet0043-1143.085481AMP-dependent synthetase and ligase family
Bpet0044-1133.576776putative aminoglycoside phosphotransferase
Bpet0045-1143.900774acyl-CoA dehydrogenase
Bpet00460124.1175642-deoxy-D-gluconate 3-dehydrogenase
Bpet00471124.109378hypothetical protein
Bpet00483144.771005hypothetical protein
Bpet00494154.928781putative secreted protein
Bpet00503154.674868Acyl-CoA synthetase
Bpet00512183.578273putative secreted protein
Bpet00521193.549069hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0041ALARACEMASE362e-04 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 35.9 bits (83), Expect = 2e-04
Identities = 31/158 (19%), Positives = 58/158 (36%), Gaps = 12/158 (7%)

Query: 31 VLDLDAFESNLRQMQAWADRHGV--ALRPHAKAHKCPEIARRQLALGARGICCQKVSEAL 88
LDL A + NL ++ A V ++ +A H I A + + EA+
Sbjct: 8 SLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLN--LEEAI 65

Query: 89 PFVAAGI-DDVHISNEVVGAAKLALLAQLARAARVSVCVDDAGNLADISAAMARAQAQVE 147
G + + A L + R++ CV L + A R +A ++
Sbjct: 66 TLRERGWKGPILMLEGFFHAQDLEIYD----QHRLTTCVHSNWQLKALQNA--RLKAPLD 119

Query: 148 VLVELDVGQGRCGVPDAAAAVALARQAQALPGVTFGGL 185
+ ++++ G R G + +Q +A+ V L
Sbjct: 120 IYLKVNSGMNRLGFQPDRVL-TVWQQLRAMANVGEMTL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0046DHBDHDRGNASE1192e-34 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 119 bits (298), Expect = 2e-34
Identities = 70/252 (27%), Positives = 120/252 (47%), Gaps = 10/252 (3%)

Query: 5 LKSKTALVTGAYSGLGRHFAGLLAGAGARVALCGRRTELGHEVAEAIRQQGGQACVVPMD 64
++ K A +TGA G+G A LA GA +A E +V +++ + A P D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 65 VTRPDSVQAAIDAAASELGPLDIVINNAGVALSEPALDISEQAWTGLIDVNLNGAWRVAQ 124
V ++ E+GP+DI++N AGV +S++ W VN G + ++
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 125 ASARHFRAQGRPGSIVNIASILGQRVASHVAPYAAAKAGLLHLTRALALEWARHGIRVNA 184
+ +++ + R GSIV + S + +A YA++KA + T+ L LE A + IR N
Sbjct: 126 SVSKYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 185 LAPGYIATDLNREFFASPAGEALIKR---------VPQRRLGQPQDLDGPLLLLASDASA 235
++PG TD+ +A G + + +P ++L +P D+ +L L S +
Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAG 244

Query: 236 FMTGSVIDVDGG 247
+T + VDGG
Sbjct: 245 HITMHNLCVDGG 256


2Bpet0166Bpet0177Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet01662180.065560dihydroxy-acid dehydratase
Bpet0167322-1.251308hypothetical protein
Bpet0168524-2.797666cytochrome c oxidase, subunit II
Bpet0169421-0.733825cytochrome c oxidase, subunit I
Bpet01705170.506944hypothetical protein
Bpet01714151.113605hypothetical protein
Bpet01724141.344240putative cytochrome c oxidase, subunit III
Bpet01733141.823721hypothetical protein
Bpet01743122.303276hypothetical protein
Bpet01751141.942178hypothetical protein
Bpet01761122.272580putative cytochrome oxidase assembly protein
Bpet01772122.272462protoheme IX farnesyltransferase
3Bpet0187Bpet0240Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0187-217-3.354357hypothetical protein
Bpet0188-119-3.211884hypothetical protein
Bpet0189-120-3.455343glycosyltransferase
Bpet0190020-3.283741autotransporter
Bpet0191022-4.155599rod shape-determining protein MreB
Bpet0192026-4.666787hypothetical protein
Bpet0193026-4.557845autotransporter
Bpet0194335-8.318516transposase
Bpet0195339-8.872058transposase
Bpet0196346-10.304406two-component response regulator
Bpet0197434-9.385947putative transposase
Bpet0198238-9.604150transposase
Bpet0199345-9.796007putative dehydrogenase
Bpet0200445-9.387738putative DNA-binding protein
Bpet0201545-9.013041putative transposase
Bpet0202343-8.801255transposase
Bpet0203546-9.195678hypothetical protein
Bpet0204549-9.861138hypothetical protein
Bpet0205651-10.478042hypothetical protein
Bpet0206645-9.292607hypothetical protein
Bpet0207542-9.228118transposase
Bpet0208647-10.122714putative transposase
Bpet0209648-9.860940putative transposition protein
Bpet0210440-7.665769Tn7-like transposition protein D
Bpet0211230-5.286248transposase
Bpet0212237-7.206573transposase
Bpet0213445-9.083580transposition protein, TnsC-related protein
Bpet0214545-8.746574transposase
Bpet0215544-8.720129putative transposase
Bpet0216748-10.024506putative transposase
Bpet0217851-11.436549hypothetical protein
Bpet0218851-11.562317putative Tn7-like transposition protein B
Bpet0219951-11.786905hypothetical protein
Bpet0220950-12.216006IS511, transposase OrfB
Bpet02211050-12.453417IS511, transposase OrfA
Bpet0222951-12.390869helicase, putative
Bpet0223849-12.755160hypothetical protein
Bpet0224648-11.835822hypothetical protein
Bpet0225646-11.067720hypothetical protein
Bpet0226444-8.131447putative transposase
Bpet0227440-6.510235putative transposase
Bpet0228431-6.555961IS1404 transposase
Bpet0229327-5.011151hypothetical protein
Bpet0230426-4.641564hypothetical protein
Bpet0231326-4.413307transcriptional regulator
Bpet0232227-4.405640hypothetical protein
Bpet0233227-4.487202NDP-sugar dehydrogenase
Bpet0234328-4.350098hypothetical protein
Bpet0235228-3.509068glycosyltransferase
Bpet0236231-4.169683outer membrane protein involved in
Bpet0237327-3.306316carbohydrate export ABC transporter
Bpet0238222-1.270764ATPase component of an ABC polysaccharide
Bpet0239221-0.619571MPA2 protein component of an ABC-type
Bpet02402180.404038hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0190PERTACTIN423e-05 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 41.6 bits (97), Expect = 3e-05
Identities = 99/477 (20%), Positives = 154/477 (32%), Gaps = 79/477 (16%)

Query: 350 SVQTSGEGSHGAY---AHDGGTIVLNGDSLSAGGNHAYGMYAKDPGSAIDATNVTVNTEG 406
S+ +GE HG + + G G ++ G A G+ ++P + + N +V + G
Sbjct: 40 SIIKAGERQHGIHIKQSDGAGVRTATGTTIKVSGRQAQGVLLENPAAELRFQNGSVTSSG 99

Query: 407 LYGFGARAENGGAITLKGGSISTDNATGQGTQDGDGSRAYALSADGANSSISAQDGVVIS 466
G +T+K G + D+AT D AL G + S D +
Sbjct: 100 QLFDEGVRRFLGTVTVKAGKLVADHATLANVSDTRDDDGIALYVAGEQAQASIADSTLQG 159

Query: 467 TKGQRA-YGAYAT--------NGGHI---------ELGGGSVTTQGFMAYGLYASGNGST 508
G R GA T G HI +L V + ASG +
Sbjct: 160 AGGVRVERGANVTVQRSTIVDGGLHIGTLQPLQPEDLPPSRVVLGDTSVTAVPASGAPAA 219

Query: 509 VDANGVDITT------SGGVGDGVWAYQGGTVNLNGGSVTVNGEPNANSPHETANGLVAV 562
V G + T +GG GV A G V+L ++ P A G V
Sbjct: 220 VFVFGANELTVDGGHITGGRAAGVAAMDGAIVHLQRATIRRGDAP--------AGGAVPG 271

Query: 563 GGTGSAAAGTINASDLSIVTRGANSAGAKAGATVDTDNTYGVINLERSTITVQGQAAVAA 622
G A G D + ++L +S + A A
Sbjct: 272 GAVPGGA--------------VPGGFGPLLDGWYGVDVSDSTVDLAQSIVEAPQLGA-AI 316

Query: 623 EINYGSTLTATDSTLVSEQGDGIVLNDNASVSLASTRVEAAGASLVSNLNAAGQTQNITV 682
G+ +T + +L + G+ I A R AS +S AG
Sbjct: 317 RAGRGARVTVSGGSLSAPHGNVIETGGGA-------RRFPPPASPLSITLQAGAR----- 364

Query: 683 GSGSNLTQNNGTLLQVNRGQEGMDGIVNLTLAAGSSSSGDVVDLDGLDQDSGLRDGGGKT 742
G L V LTLA G+ GD+V + G
Sbjct: 365 AQGRALLYRVLPE------------PVKLTLAGGAQGQGDIVATELPPIPGAS---SGPL 409

Query: 743 NFTVAQGASWIGIVRGINDLAAEDGGEIINVGGEPIAGNVTGGQDSTIVFQNGADIG 799
+ +A A W G R ++ L+ ++ ++ G + D ++ FQ A+ G
Sbjct: 410 DVALASQARWTGATRAVDSLSIDNATWVMT--DNSNVGALRLASDGSVDFQQPAEAG 464


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0191SHAPEPROTEIN488e-177 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 488 bits (1258), Expect = e-177
Identities = 246/347 (70%), Positives = 291/347 (83%), Gaps = 1/347 (0%)

Query: 1 MIGFMRSYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHEGGPNGKKIIQAVGHEA 60
M+ R FS DL+IDLGTANTLIYV+G+GIVL+EPSVVAIR + + K + AVGH+A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVA-AVGHDA 59

Query: 61 KQMLGRVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHPRNMLAPSPRIIVCVPCGST 120
KQMLGR PGNI AIRPMKDGVIADF VTE+ML+ FI+ VH + + PSPR++VCVP G+T
Sbjct: 60 KQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGAT 119

Query: 121 QVERRAIRESALGAGASHVYLIEEPMAAAIGAGLAVSDASGSMVVDIGGGTTEVAVISLG 180
QVERRAIRESA GAGA V+LIEEPMAAAIGAGL VS+A+GSMVVDIGGGTTEVAVISL
Sbjct: 120 QVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN 179

Query: 181 GMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEPTAELIKKEIGSAFPGSEVREIEVKGR 240
G+VY SVR+GGD+FDEAI+NY+RRNYG LIGE TAE IK EIGSA+PG EVREIEV+GR
Sbjct: 180 GVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGR 239

Query: 241 NLAEGVPRSFTVSSNEILESLTDPLNQIVSAVKIALEQTPPELGADITDKGIALTGGGAL 300
NLAEGVPR FT++SNEILE+L +PL IVSAV +ALEQ PPEL +DI+++G+ LTGGGAL
Sbjct: 240 NLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL 299

Query: 301 LRDLDRLLQEETGLPVVVAEESLTCVVRGCGQALDQLERLGEIFLRD 347
LR+LDRLL EETG+PVVVAE+ LTCV RG G+AL+ ++ G +
Sbjct: 300 LRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSE 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0193PRTACTNFAMLY445e-06 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 43.9 bits (103), Expect = 5e-06
Identities = 94/459 (20%), Positives = 156/459 (33%), Gaps = 75/459 (16%)

Query: 215 GASASVDRSRILTRGRDADGVSVK---HGGIAVVSKSSIQTSGRSADGISVSGE------ 265
A A + I+ G G+ ++ GG+ S ++I+ SGR A GI +
Sbjct: 31 AAHADWNNQSIVKTGERQHGIHIQGSDPGGVRTASGTTIKVSGRQAQGILLENPAAELQF 90

Query: 266 RSLVVGDSLKISTKGENSHGVDVEDGGTLLLSRGDVESKGKDGRGVAIDDGGRAVIVGSK 325
R+ V S ++S G V K G+ VA + +
Sbjct: 91 RNGSVTSSGQLSDDGIRRFLGTVTV---------------KAGKLVA-----DHATLANV 130

Query: 326 VSASGDRGIALHVDDKDSQAIVIGSRLQSSGRSGQAVLIEDGADALIAGSTIVADGLGV- 384
D GIAL+V + +QA + S LQ +G V IE GA+ + S IV GL +
Sbjct: 131 GDTWDDDGIALYVAGEQAQASIADSTLQGAG----GVQIERGANVTVQRSAIVDGGLHIG 186

Query: 385 QVQGKGSQLIGLDVDIDAESEARYRQSREPALGLRVEDQAKALLVGGSIQA--------- 435
+Q + + + ++ + + V ++ L GG I
Sbjct: 187 ALQSLQPEDLPPSRVVLRDTNVTAVPASGAPAAVSVLGASELTLDGGHITGGRAAGVAAM 246

Query: 436 -------------KGDQATGVSVGGSGSLVFAAG-----TDIRATGDDSTGMRVSRDARA 477
+GD G +V G A D G+ VS +
Sbjct: 247 QGAVVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPGGFGPVLDGWYGVDVS-GSSV 305

Query: 478 ILVGSDIQGGGKGLDI--TKGGQAGTVGGTVTATDARGVALSVSGRDSVAATIGTNLTAD 535
L S ++ G I +G + GG+++A + +G A L+
Sbjct: 306 ELAQSIVEAPELGAAIRVGRGARVTVSGGSLSAPHG---NVIETGGARRFAPQAAPLSIT 362

Query: 536 GQDGVAVEVRDAGRAYLIDSSLNARAVGLRAEGKDA--SVVSVGSSITAGTEIVPVGRAY 593
Q G G+A L + L G DA +V+ GT I P+ A
Sbjct: 363 LQAG----AHAQGKALLYRVLPEPVKLTL-TGGADAQGDIVATELPSIPGTSIGPLDVAL 417

Query: 594 SEPAVGV-ASDTGATVILIGGDVTALGDNSVAAQATSGD 631
+ A A+ ++ + +++V A + D
Sbjct: 418 ASQARWTGATRAVDSLSIDNATWVMTDNSNVGALRLASD 456


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0194HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0208HTHTETR260.025 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 25.7 bits (56), Expect = 0.025
Identities = 9/47 (19%), Positives = 18/47 (38%), Gaps = 4/47 (8%)

Query: 21 GVSVEEVCRKVGVSQATFYAWKKKYAGVGVS----ELRRLRQLEDEN 63
S+ E+ + GV++ Y K + + + +LE E
Sbjct: 31 STSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELEY 77


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0212HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0224OUTRMMBRANEA366e-05 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 36.4 bits (84), Expect = 6e-05
Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 6/65 (9%)

Query: 98 FAFGTWRLNAQQESVLRQFVPEILTIANDELGKNILKRVVIEGYTDKTGSYLTNLNLSLQ 157
F F L + ++ L Q ++ + + VV+ GYTD+ GS N LS +
Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKD------GSVVVLGYTDRIGSDAYNQGLSER 276

Query: 158 RSQKV 162
R+Q V
Sbjct: 277 RAQSV 281


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0225RTXTOXINA300.028 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 30.3 bits (68), Expect = 0.028
Identities = 64/338 (18%), Positives = 124/338 (36%), Gaps = 52/338 (15%)

Query: 288 LGEVIASSIDQSLKTPLDEIASSVKSASGDQSKAAIDMLNDVMVHFSQRLNDLFGGQISG 347
+ + + I +L++ A+ + SA G +K A+ + + RL I
Sbjct: 1 MTTITTAQIKSTLQSAKQSAANKLHSA-GQSTKDALKKAAEQTRNAGNRL-------ILL 52

Query: 348 INELNKETAQSMQDAVTALNTLLGKVEDSGKR--ATEEMALKMASSIQAMEERQASINAQ 405
I + K S+ D V + L +V+ K A + A + + ER +I
Sbjct: 53 IPKDYKGQGSSLNDLVRTADELGIEVQYDEKNGTAITKQVFGTAEKLIGLTERGVTI--- 109

Query: 406 TQDFVDQIRKLVESSQSETQQKLQGTLESVGQQMT---TILETLNTSQAQVFAQNQAREQ 462
F Q+ KL++ Q + L G E++G + IL T + + E
Sbjct: 110 ---FAPQLDKLLQKYQ-KAGNILGGGAENIGDNLGKAGGILSTFQNFLGTALSSMKIDEL 165

Query: 463 AMADRASHTVSQMSGSVEAAIQEISAASKAMTESVSALSSATSTSVEKMNAGAERLGIAA 522
++ VS S +KA E ++ L ++ +N+ +++L
Sbjct: 166 IKKQKSGGNVSS------------SELAKASIELINQLVDTVASLNNNVNSFSQQLNTLG 213

Query: 523 SSFANAGERVAEVITQTTSIGNKLTEVSSSLSTSSTSLHEALGDYRVQREALAHVLAEVR 582
S +N +GNKL + + L L + ++ +L+ +
Sbjct: 214 SVLSNT--------KHLNGVGNKLQNLPN-LDNIGAGL-----------DTVSGILSAIS 253

Query: 583 EMIGLAGKEANITADALQRIEVSTTKLGLAQKAADEYL 620
L+ +A+ A +E++T LG K +Y+
Sbjct: 254 ASFILSNADADTRTKAAAGVELTTKVLGNVGKGISQYI 291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0227ARGDEIMINASE290.005 Bacterial arginine deiminase signature.
		>ARGDEIMINASE#Bacterial arginine deiminase signature.

Length = 409

Score = 28.6 bits (64), Expect = 0.005
Identities = 5/26 (19%), Positives = 18/26 (69%)

Query: 19 LSNLLAESVLDNAALKDQLVDGFVAD 44
+ +L++E ++ + AL+++ + F+ +
Sbjct: 72 IEDLISEVLVSSVALENKFISQFILE 97


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0237ABC2TRNSPORT290.031 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 29.1 bits (65), Expect = 0.031
Identities = 22/109 (20%), Positives = 43/109 (39%), Gaps = 3/109 (2%)

Query: 335 VVLMGGCGAAMGVLFGAIATFWHFYLRLAPVIERFLQIFSGVFFVSEQLPEQLRIWILWS 394
+ L G A++G++ A+A + +++ ++ + SG F +QLP + +
Sbjct: 154 IALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQTAARFL 213

Query: 395 PFAHGMQLLRSAYFSAYTSQDA---SLGYFLTSLVFLMVLALAAERLAR 440
P +H + L+R + F + AL RL R
Sbjct: 214 PLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTALLRRRLLR 262


4Bpet0390Bpet0407Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet03901103.139965putative secreted protein
Bpet0391193.246320Zinc-binding dehydrogenase family protein
Bpet03921102.484264acyl-CoA transferase/carnitine dehydratase
Bpet03930111.563447LysR family transcriptional regulator
Bpet03940121.806850putative outer membrane lipoprotein
Bpet03950111.939666glutamate--cysteine ligase
Bpet03963142.232444hypothetical protein
Bpet03971121.980734LuxR family transcriptional regulator
Bpet03982141.926522LysR family transcriptional regulator
Bpet03993172.823782fumarate reductase iron-sulfur protein
Bpet04002182.695693hypothetical protein
Bpet04011183.622774succinate dehydrogenase cytochrome b-556
Bpet04022183.889210fumarate hydratase alpha subunit
Bpet04031164.513031L-tartrate dehydratase, subunit B
Bpet04042145.197238putative succinate dehydrogenase flavoprotein
Bpet04050135.272985putative secreted protein
Bpet04060154.922980acetolactate synthase large subunit
Bpet0407-2173.368061hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0394BCTLIPOCALIN757e-20 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 75.4 bits (185), Expect = 7e-20
Identities = 44/153 (28%), Positives = 73/153 (47%), Gaps = 8/153 (5%)

Query: 25 PKAVAI----DPQRYAGKWYEIARLPTPLQRRCVGDVTVEYTVAPQSALHIDNRCRT-KH 79
P++V + Y GKWYE+ARL +R + VT EY V + + NR + +
Sbjct: 20 PESVKPVSDFELNNYLGKWYEVARLDHSFERG-LSQVTAEYRVRNDGGISVLNRGYSEEK 78

Query: 80 GDVAAMSGLAVPREQAAGAQYRAEFLQP-TPDYWIIGLDSE-YRWAVVGSPDRKTLWILS 137
G+ G A + + F P Y + LD E Y +A V P+ + LW+LS
Sbjct: 79 GEWKEAEGKAYFVNGSTDGYLKVSFFGPFYGSYVVFELDRENYSYAFVSGPNTEYLWLLS 138

Query: 138 RTPQLPASLLEQARQAARAQGYRLDELRYTPQR 170
RTP + +L++ + ++ +G+ + L Y Q+
Sbjct: 139 RTPTVERGILDKFIEMSKERGFDTNRLIYVQQQ 171


5Bpet0436Bpet0459Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0436-1133.789736thioredoxin
Bpet04370134.609165hypothetical protein
Bpet04380123.848745phosphoheptose isomerase
Bpet04391124.638658hypothetical protein
Bpet04402113.975232putative tetrapyrrole methylase
Bpet04412132.568580methylated-DNA--protein-cysteine
Bpet04421122.030858putative lipoprotein
Bpet04430120.683498hypothetical protein
Bpet0444-1131.034144cytochrome C
Bpet0445-2150.459031cytochrome C
Bpet0446-117-0.396749HPr kinase/phosphorylase
Bpet0447-1190.992140nitrogen regulatory IIA protein
Bpet0448-1222.077482putative sigma-54 modulation protein
Bpet04490223.160839Sulfate/thiosulfate import ATP-binding protein
Bpet0450-1192.717827hypothetical protein
Bpet04510171.744506hypothetical protein
Bpet04520151.8334893-deoxy-manno-octulosonate-8-phosphatase
Bpet04531131.272048NDP-sugar epimerase
Bpet0454-1112.136169phosphoribosylglycinamide formyltransferase 2
Bpet04551153.667486putative transcriptional regulator
Bpet04562154.059025cytochrome D ubiquinol oxidase subunit I
Bpet04573144.707319cytochrome D ubiquinol oxidase subunit II
Bpet04582145.279931hypothetical protein
Bpet04591114.148921ATP-binding component of cytochrome-related
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0446NUCEPIMERASE280.043 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 28.2 bits (63), Expect = 0.043
Identities = 9/31 (29%), Positives = 16/31 (51%)

Query: 149 VLITGESGLGKSELALELISRGHGLVADDAV 179
L+TG +G ++ L+ GH +V D +
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNL 33


6Bpet0517Bpet0567Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0517-2153.153505putative gentisate 1,2-dioxygenase
Bpet0518-2122.961003LysR family transcriptional regulator
Bpet0519-2122.612680hypothetical protein
Bpet0520-1144.300751hypothetical protein
Bpet0521-1123.714869putative secreted protein
Bpet0522-2122.175153hypothetical protein
Bpet0523-3121.619655hypothetical protein
Bpet0524-3141.730154hypothetical protein
Bpet0525-2143.125245glycerol kinase
Bpet0526-2152.892144putative transport protein
Bpet0527-1163.910448hypothetical protein
Bpet05280144.963653pantoate--beta-alanine ligase
Bpet05291124.715908putative regulatory protein
Bpet05300134.367875putative lipoprotein
Bpet05310142.567200putative lipoprotein
Bpet05321141.930278prepilin signal peptidase
Bpet05330152.161330dephospho-CoA kinase
Bpet05340142.304714hypothetical protein
Bpet05350152.473020HlyD family secretion protein
Bpet05360162.328326AcrB/AcrD/AcrF family protein
Bpet0537-1142.668792AcrB/AcrD/AcrF family protein
Bpet0538-2153.643471putative outer membrane efflux protein
Bpet0539-3171.770261hypothetical protein
Bpet0540-3171.114765hypothetical protein
Bpet05410161.479676bifunctional ornithine
Bpet05422172.062808MarR family transcriptional regulator
Bpet05433152.431106putative esterase/lipase
Bpet0544-1161.814916putative ABC transporter substrate binding
Bpet05450162.922075branched chain amino acid ABC transporter
Bpet0546-1163.065555branched chain amino acid ABC transporter
Bpet0547-1153.422609putative branched-chain amino acid ABC
Bpet0548-1163.167887putative branched-chain amino acid ABC
Bpet0549-1162.806464salicylyl-CoA 5-hydroxylase
Bpet0550-1163.250504putative oxidoreductase
Bpet0551-1163.487084enoyl-CoA hydratase
Bpet0552-2163.655606putative acyl-CoA dehydrogenase
Bpet0553-2184.090905acid-coenzyme A ligase
Bpet0554-1164.8286744-hydroxybenzoyl CoA thioesterase
Bpet0555-1165.071581hypothetical protein
Bpet0556-1154.881602hypothetical protein
Bpet0557-3164.367441glutamate-1-semialdehyde aminotransferase
Bpet0558-2144.453695thiamine-phosphate pyrophosphorylase
Bpet05590133.088996phosphomethylpyrimidine kinase
Bpet0560-1161.794609high molecular weight rubredoxin
Bpet05610171.606921hypothetical protein
Bpet05621182.790777Holliday junction resolvase-like protein
Bpet05632173.124866putative secreted acyltransferase
Bpet05641182.917175diadenosine tetraphosphatase
Bpet05651172.985703hypothetical protein
Bpet05660183.636686monofunctional biosynthetic peptidoglycan
Bpet0567-1153.713801shikimate 5-dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0519THERMOLYSIN300.032 Thermolysin metalloprotease (M4) family signature.
		>THERMOLYSIN#Thermolysin metalloprotease (M4) family signature.

Length = 544

Score = 30.4 bits (68), Expect = 0.032
Identities = 34/159 (21%), Positives = 55/159 (34%), Gaps = 16/159 (10%)

Query: 598 AANGEIRNITGLFDETLNDGEKRLVTGA--FEETIDGDVTQTIKGGGTFTETIMANHIHD 655
A N + +I G E + G + + GD +++ + + +
Sbjct: 391 AINEAMSDIFGTLVEFYANRNPDWEIGEDIYTPGVAGDALRSMSDPAKYGDPDHYSK--R 448

Query: 656 LTGTQDSTITGAVTHTVNGTFTQTVTEPLTVNADTSMTVNTPSWTVSGAKQAFWTGSFLR 715
TGTQD+ G V HT +G + L V+ + F+
Sbjct: 449 YTGTQDN---GGV-HTNSGIINKAAY--LLSQGGVHYGVSVTGIGRDKMGKIFYRALVYY 502

Query: 716 GTP------ARATVVLAAADLWGVRQQVYAGINSQWSTV 748
TP RA V AAADL+G Q + ++ V
Sbjct: 503 LTPTSNFSQLRAACVQAAADLYGSTSQEVNSVKQAFNAV 541


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0526TCRTETB1304e-35 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 130 bits (329), Expect = 4e-35
Identities = 77/401 (19%), Positives = 160/401 (39%), Gaps = 14/401 (3%)

Query: 88 FMQMLDSTVVATALPVMAQALGSTAVRLNVAITSYLLAVAVFVPVSGWAADRYGARRVFM 147
F +L+ V+ +LP +A N T+++L ++ V G +D+ G +R+ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 148 AAIALFTLSSVACALSQNLTQ-LVLARIVQGIAGAMMVPVGRIILLRVVPKQDLLKAMSF 206
I + SV + + L++AR +QG A + +++ R +PK++ KA
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 207 LSIPALLGPVIGPPLGGFMVTYMSWHWIFLINIPIGILGIALVRRYVPEIREASTPRLDW 266
+ +G +GP +GG + Y+ HW +L+ IP+ + + + D
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHYI--HWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 267 LGFLLSAICLATLVSAFEALGHSLIPPLAVASLAATGLLCGALYVLHARRAAHPIIDLTL 326
G +L ++ + + L S +L ++V H R+ P +D L
Sbjct: 202 KGIILMSVGIVFFM---------LFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGL 252

Query: 327 LREPTFAISVLGGNLCRFAVGAMPFLLAVQLQVGFGLTPFSAG-LITFASAAGALLMKFV 385
+ F I VL G + V ++ ++ L+ G +I F ++ ++
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312

Query: 386 ATPIVQRFGFRRVLTVNAVLTGLFIVACAAFTATTPVWLLIGILLAGGFFRSLQFTGVNT 445
+V R G VL + + + + TT ++ I I+ G + T ++T
Sbjct: 313 GGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTK-TVIST 371

Query: 446 LTYADIPPERMSSASSFAAMAQQLGISLGVGVAAVTLNLSM 486
+ + + + + S L G+ + L++ +
Sbjct: 372 IVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPL 412


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0532PREPILNPTASE2376e-80 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 237 bits (607), Expect = 6e-80
Identities = 124/270 (45%), Positives = 157/270 (58%), Gaps = 1/270 (0%)

Query: 5 FAVDPGWAIAMAALLGLVVGSWLSVPAHRLPRMMEREWLQQYQEFRPAASGPEPAASAYT 64
P ++ L L++GS+L+V HRLP M+EREW +Y+ + Y
Sbjct: 8 AHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGVDEP-PYN 66

Query: 65 LWRPGWHCPACAAPVRGWRRLPVLGWLLLRGRCGACGEAIGWRYPAVEVTAALLFALCAW 124
L P CP C P+ +P+L WL LRGRC C I RYP VE+ ALL A
Sbjct: 67 LMVPRSCCPHCNHPITALENIPLLSWLWLRGRCRGCQAPISARYPLVELLTALLSVAVAM 126

Query: 125 RFGPTPIALCAMGLCAALLALAWIDLQTSLLPDAITLPLAWAGLLVNLGGALAPLPLAVL 184
P L A+ L L+AL +IDL LLPD +TLPL W GLL NL G L AV+
Sbjct: 127 TLAPGWGTLAALLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVI 186

Query: 185 GAVVGYVFLWLLFHMFRLLTGREGMGYGDFKLLAALGAWFGLAALPGLLLVASLAGVAGA 244
GA+ GY+ LW L+ F+LLTG+EGMGYGDFKLLAALGAW G ALP +LL++SL G
Sbjct: 187 GAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMG 246

Query: 245 GILRLTGHARRGQPLPFGPYLALAGMVMLL 274
L L + + +P+PFGPYLA+AG + LL
Sbjct: 247 IGLILLRNHHQSKPIPFGPYLAIAGWIALL 276


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0535RTXTOXIND448e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.0 bits (104), Expect = 8e-07
Identities = 24/194 (12%), Positives = 53/194 (27%), Gaps = 37/194 (19%)

Query: 8 TPTPRRKRRLAAIVLLLLAAAVAAWLLFKPGGSQQAATRGGRGFGGAATMNMPVPVRVAE 67
TP RR R +A ++ L A +
Sbjct: 51 TPVSRRPRLVAYFIMGFLVIAFI-LSVLGQ------------------------------ 79

Query: 68 AGTQDINIVLRALGTVTAY-NTVTVRSRVDGELVRVAFAEGQRVKAGDLLAQIDPRPFEV 126
+ IV A G +T + ++ + + + EG+ V+ GD+L ++ E
Sbjct: 80 -----VEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 127 ALAQAQGQQQQNQALLANARRDLQRYQTLFKQDSIARQQLDTQAALVRQYEGTQKIDQAA 186
+ Q Q + + + + + + Q + + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 187 VDNAKLQLSYTRIT 200
+ Q +
Sbjct: 195 FSTWQNQKYQKELN 208


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0536ACRIFLAVINRP8440.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 844 bits (2181), Expect = 0.0
Identities = 289/1036 (27%), Positives = 506/1036 (48%), Gaps = 29/1036 (2%)

Query: 4 SRLFILRPVATTLSMVAILIAGFIAYRLLPVSALPEVDYPTIQVVTLYPGASPDVMTSLV 63
+ FI RP+ + + +++AG +A LPV+ P + P + V YPGA + V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 64 TSPLERQFGQMPGLNQMSSTS-SGGASVITLQFSLDLSLDVAEQEVQAAINAASNLLPSD 122
T +E+ + L MSSTS S G+ ITL F D+A+ +VQ + A+ LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 123 LPVPPIYNKVNPADAPVLTLAISS--PTMPLPQVRDLVDTRMAQKLSQVPGVGLVGVAGG 180
+ I + + ++ S P + D V + + LS++ GVG V + G
Sbjct: 122 VQQQGIS-VEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QRPAVRIQVNPRALAAAGMSLADLRTAVVGANVNQPKGNLDGP------ERSTTIDANDQ 234
Q A+RI ++ L ++ D+ + N G L G + + +I A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 235 LKSPTDYNDLII-AYRNNAPLRLSDVATAVQGAEDVRQAAWAGGQPAILLNVQRQPGANV 293
K+P ++ + + + + +RL DVA G E+ A G+PA L ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 IDVVDRIRAMLPQAQAALPATLDVSIVSDRTQTIRASVSDVQFEMMLAVALVVMVTFLFL 353
+D I+A L + Q P + V D T ++ S+ +V + A+ LV +V +LFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 RSLTATFIPSVVVPLSLVGTFGIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENIARH 413
+++ AT IP++ VP+ L+GTF I+ G+SIN LT+ + +A G +VDDAIV++EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 L-EQGETPMQAALKGAAQIGFTLISLTFSLIAVLIPLLFMTEVVGRLFREFAITLAVAIL 472
+ E P +A K +QI L+ + L AV IP+ F G ++R+F+IT+ A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 ISLVVSLTLTPMMCARLLRPESEQRH---GRFHQATGAFIDRTIAHYDRMLQWVLAHQRL 529
+S++V+L LTP +CA LL+P S + H G F D ++ HY + +L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 530 TLLVALGTFVLTALLYIAIPKGFFPQQDTGMIQAITQAPASVSFPAMAQRQQEAARIVLQ 589
LL+ +L++ +P F P++D G+ + Q PA + + + L+
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 590 D--PDVESVSSFIGVDGTNATLNTGRMQIALKPHGERDGD---LAEVTRRLQQALDAQQG 644
+ +VESV + G + N G ++LKP ER+GD V R + L +
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 645 LKVYMQPVQDLTIEDRVSRTQYQMTL---SNPDIAVLAEWAPKLVERLSQLP-ELTDVAH 700
V P I + + T + L + L + +L+ +Q P L V
Sbjct: 660 GFVI--PFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRP 717

Query: 701 DLQDDGLQTWVDIDRDAAARLGISTSAIDEALYNAFGQRLISTIFTQSNQYRVVLEVLPQ 760
+ +D Q +++D++ A LG+S S I++ + A G ++ + ++ ++ +
Sbjct: 718 NGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAK 777

Query: 761 FRQSPEALGQIHLATESGTLVPLSAVAHISQGRTMLAINRLDQFPMTTVSFNLAPGASLS 820
FR PE + ++++ + +G +VP SA + R + P + APG S
Sbjct: 778 FRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG 837

Query: 821 AAVDAIAEAEAGIGMPASIETRYQGAALAFQNSLSSTLWLILAAVITMYIVLGVLYESYI 880
A+ + + +PA I + G + + S + L+ + + +++ L LYES+
Sbjct: 838 DAMALMENLASK--LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWS 895

Query: 881 HPVTILSTLPSAAVGALLALMISGTELDMIGIIGIILLIGIVKKNAIMMIDFALEAERKR 940
PV+++ +P VG LLA + + D+ ++G++ IG+ KNAI++++FA + K
Sbjct: 896 IPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKE 955

Query: 941 GLAPRAAIHEAALLRFRPILMTTLAALFGALPLMLSTGTGAELRQPLGLVMVGGLLLSQV 1000
G A A +R RPILMT+LA + G LPL +S G G+ + +G+ ++GG++ + +
Sbjct: 956 GKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATL 1015

Query: 1001 LTLFTTPVIYLMFDRL 1016
L +F PV +++ R
Sbjct: 1016 LAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0537ACRIFLAVINRP7910.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 791 bits (2045), Expect = 0.0
Identities = 291/1032 (28%), Positives = 504/1032 (48%), Gaps = 28/1032 (2%)

Query: 7 FIVRPVATVLLCLGLVLAGVLSFRLLPVAPLPEVDLPIISVTANLPGASPETMASSVATP 66
FI RP+ +L + L++AG L+ LPVA P + P +SV+AN PGA +T+ +V
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 67 LERSLGSIAGVTEMTSR-NSQGSTRITLQFDLSRDIDGAARDVQAAINAARSLLPTGLRS 125
+E+++ I + M+S +S GS ITL F D D A VQ + A LLP ++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ- 123

Query: 126 NPTYHKVNPSSAPIMVLALTSDT--LSQGRLYDLASTIVAQKLAQVNGVGEVTVGGSSLP 183
SS+ +MV SD +Q + D ++ V L+++NGVG+V + G+
Sbjct: 124 QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY- 182

Query: 184 AVRVNLIPGALSSRGVSLDEVRATLTEANANRPKGVVENDRY------HWQIMASDQLER 237
A+R+ L L+ ++ +V L N G + + I+A + +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKN 242

Query: 238 AEQYRPLVV-AWRDGAAVRLSDVATVEDSVEDLFQTGFYNNRQAILLILRRQADANIIET 296
E++ + + DG+ VRL DVA VE E+ N + A L ++ AN ++T
Sbjct: 243 PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDT 302

Query: 297 VEAIRAQLPQLAALLPGDVDMTVAQDRTPSIRASLHEAELTLVVAVALVMLVVLLFLRHW 356
+AI+A+L +L P + + D TP ++ S+HE TL A+ LV LV+ LFL++
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 357 RAALIPSVAVPVSLVGTFCIMYLCGYTLNTISLMALIVATGFVVDDAIVVLENIMRHI-E 415
RA LIP++AVPV L+GTF I+ GY++NT+++ +++A G +VDDAIVV+EN+ R + E
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 416 QGASPMRAALRGSREVGFTVLSMSLSLVAVFIPILLMGGVVGRLFREFAVTLSAAIMVSL 475
P A + ++ ++ +++ L AVFIP+ GG G ++R+F++T+ +A+ +S+
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 476 VVSLTLTPMMCARLLR--TQDHGRAPGRLSRAIGRGFDAVLARYRRSLSWALAHGRIMLL 533
+V+L LTP +CA LL+ + +H G FD + Y S+ L LL
Sbjct: 483 LVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLL 542

Query: 534 LLAAAIGLNVYLYAVVPKGFFPQQDTGQLLGFFRVDQGTSFQATVPKLEYFRKVILSDP- 592
+ A + V L+ +P F P++D G L ++ G + + T L+ L +
Sbjct: 543 IYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEK 602

Query: 593 ----AVASITVHAGGRGGSNSSFMSIQLKPQAERKASANDV---VNRLRGRLQNTPGARV 645
+V ++ + N+ + LKP ER N ++R + L V
Sbjct: 603 ANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFV 662

Query: 646 FLVPQQDIFLGGGQGSGSYDYTLLAGELSL-LRTWMPKV-QQAMAALPELTDVDTSVEDK 703
I G ++ AG L ++ A L V + +
Sbjct: 663 IPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLED 722

Query: 704 GRQVELVIDREAATRLGISMSDISAVLNNSFSQRQVSVMYGPLNQYHVVMGVVQRFAQDA 763
Q +L +D+E A LG+S+SDI+ ++ + V+ + + +F
Sbjct: 723 TAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLP 782

Query: 764 ESLKQVHVITQDGRRVPLAAFAHFESGNAPLSVRHNGLLAADEISFNLAPGVSLDQAIRA 823
E + +++V + +G VP +AF + L + EI APG S A+
Sbjct: 783 EDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMAL 842

Query: 824 IDAAVARIGLPSDQIQAGFLGTAAAQQEVQSQQPWLILGALVTMYIVLGILYENLVHPLT 883
++ ++ LP+ I + G + ++ +Q P L+ + V +++ L LYE+ P++
Sbjct: 843 MENLASK--LPAG-IGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 884 ILSTLPSAGIGALLALMLVGSEFTIIALIGVFLLIGIVKKNAIMMVDFALDAERRRGLSP 943
++ +P +G LLA L + + ++G+ IG+ KNAI++V+FA D + G
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGV 959

Query: 944 RDAIFEACLTRFRPIMMTTLAAIFGALPLVLATGAGVEMRQPLGVTIVGGLILSQILTLY 1003
+A A R RPI+MT+LA I G LPL ++ GAG + +G+ ++GG++ + +L ++
Sbjct: 960 VEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIF 1019

Query: 1004 TTPVVYLYLDRF 1015
PV ++ + R
Sbjct: 1020 FVPVFFVVIRRC 1031



Score = 91.8 bits (228), Expect = 8e-21
Identities = 75/502 (14%), Positives = 166/502 (33%), Gaps = 29/502 (5%)

Query: 5 APFIVRPVATVLLCLGLVLAGVLSFRLLPVAPLPEVDLPIISVTANLPGASPETMASSVA 64
+ +L+ +V V+ F LP + LPE D + LP + + V
Sbjct: 531 GKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVL 590

Query: 65 TPLER-----------SLGSIAGVTEMTSRNSQGSTRITLQFDLSRDIDGAARDVQAAIN 113
+ S+ ++ G + + G ++L+ + +G +A I+
Sbjct: 591 DQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLK--PWEERNGDENSAEAVIH 648

Query: 114 AARSLLPTGLRSNPTYHKVNPSSAPIMVLALTSDTLSQG-----RLYDLASTIVAQKLAQ 168
A+ L + + + Q L + ++
Sbjct: 649 RAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQH 708

Query: 169 VNGVGEVTVGGSS-LPAVRVNLIPGALSSRGVSLDEVRATLTEANANRPKGVVEND-RYH 226
+ V G ++ + + GVSL ++ T++ A + R
Sbjct: 709 PASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVK 768

Query: 227 WQIMASDQLER--AEQYRPLVVAWRDGAAVRLSDVATVEDSVEDLFQTGFYNNRQAILLI 284
+ +D R E L V +G V S T + + + +
Sbjct: 769 KLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWV----YGSPRLERYNGLPSM 824

Query: 285 LRRQADANIIETVEAIRAQLPQLAALLPGDVDMTVAQDRTPSIRASLHEAELTLVVAVAL 344
+ A + +A A + LA+ LP + + R S ++A + ++ +
Sbjct: 825 EIQGEAAPGTSSGDA-MALMENLASKLPAGIGYDWT-GMSYQERLSGNQAPALVAISFVV 882

Query: 345 VMLVVLLFLRHWRAALIPSVAVPVSLVGTFCIMYLCGYTLNTISLMALIVATGFVVDDAI 404
V L + W + + VP+ +VG L + ++ L+ G +AI
Sbjct: 883 VFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAI 942

Query: 405 VVLENIM-RHIEQGASPMRAALRGSREVGFTVLSMSLSLVAVFIPILLMGGVVGRLFREF 463
+++E ++G + A L R +L SL+ + +P+ + G
Sbjct: 943 LIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAV 1002

Query: 464 AVTLSAAIMVSLVVSLTLTPMM 485
+ + ++ + ++++ P+
Sbjct: 1003 GIGVMGGMVSATLLAIFFVPVF 1024


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0550DHBDHDRGNASE1031e-28 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 103 bits (257), Expect = 1e-28
Identities = 72/263 (27%), Positives = 118/263 (44%), Gaps = 18/263 (6%)

Query: 8 LEGRHALVTGGARGIGLSCAQALLQRGAAVTLLGRDRAALDAAAAALGRLG-AVRAVAAD 66
+EG+ A +TG A+GIG + A+ L +GA + + + L+ ++L A AD
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 67 VADQASVQAAFEQAAQDGGAVDILVNNAGQAASQRFERTDAALWQAMLAVNLTGTYYCIQ 126
V D A++ + ++ G +DILVN AG W+A +VN TG + +
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 127 AALPGMLAAGWGRIVNVASTAGLIGYAYVSAYCAAKHGVVGLTRALALEVARKGVTVNAV 186
+ M+ G IV V S + ++AY ++K V T+ L LE+A + N V
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 187 CPGFTETDIVRGAVDNIMQKTGRTASEARAELAARN--------PQGRLVQPEEVAEAVA 238
PG TETD MQ + ++ + P +L +P ++A+AV
Sbjct: 186 SPGSTETD---------MQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVL 236

Query: 239 WLALPASASINGQAIAVDGGEVM 261
+L + I + VDGG +
Sbjct: 237 FLVSGQAGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0560ACETATEKNASE280.001 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 27.8 bits (62), Expect = 0.001
Identities = 12/45 (26%), Positives = 19/45 (42%), Gaps = 4/45 (8%)

Query: 10 GWVYDEEAGL-PDEGIAPGTRWEDVPP---NWVCPECGARKEDFE 50
G D G P EG+A GTR + P +++ + E+
Sbjct: 220 GKSIDTSMGFTPLEGLAMGTRSGSIDPSIISYLMEKENISAEEVV 264


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0562SECA270.025 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 27.1 bits (60), Expect = 0.025
Identities = 21/72 (29%), Positives = 29/72 (40%), Gaps = 8/72 (11%)

Query: 18 IAIGNTLTRQARPLEIIFSEIREARFARIGQLLQQWQPQRVVVGLALASDGGEQPATARC 77
I +G + + LE +E E A Q + V+ L G E+ +
Sbjct: 513 IVLGGSWQAEVAALENPTAEQIEKIKADW-----QVRHDAVLEAGGLHIIGTERHES--- 564

Query: 78 RRFANQLRGRYG 89
RR NQLRGR G
Sbjct: 565 RRIDNQLRGRSG 576


7Bpet0577Bpet0592Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0577212-0.596246transmembrane protein
Bpet0578116-2.069741putative carbohydrate kinase
Bpet0579014-0.085980lipoprotein-like protein
Bpet0580013-0.264880hypothetical protein
Bpet05811130.110979hypothetical protein
Bpet0582-1131.283558ribonucleotide-diphosphate reductase subunit
Bpet0583-1142.122981ribonucleotide-diphosphate reductase subunit
Bpet0584-1134.3405352-oxoacid ferredoxin oxidoreductase
Bpet0585-1114.074551AsnC family transcriptional regulator
Bpet0586-2114.050627putative lipoprotein
Bpet0587-3143.383617putative membrane transport protein
Bpet0588-2162.169949glutamyl-tRNA synthetase
Bpet0589-1172.079340hypothetical protein
Bpet0590220-0.165759carboxylesterase
Bpet05913200.038091hypothetical protein
Bpet0592421-1.308728putative exported solute-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0579PF07132280.027 Harpin protein (HrpN)
		>PF07132#Harpin protein (HrpN)

Length = 356

Score = 27.7 bits (61), Expect = 0.027
Identities = 26/87 (29%), Positives = 36/87 (41%)

Query: 43 GGCANRSASSGVYSYDQAQREQIVRIGTVTGVRPITIQDDKSSGVGMIAGGALGGVAGNA 102
GG ++SA G S Q I+ G G+G GG GG+ G
Sbjct: 32 GGSPSQSAFGGQRSNIAEQLSDIMTTMMFMGSMMGGGLGGGLGGLGSSLGGLGGGLLGGG 91

Query: 103 VGGGTGRALATVGGAILGALAGNAVEN 129
+GGG G +L + G+ LG G A+
Sbjct: 92 LGGGLGSSLGSGLGSALGGGLGGALGA 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0584RTXTOXIND310.039 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.039
Identities = 34/288 (11%), Positives = 69/288 (23%), Gaps = 47/288 (16%)

Query: 707 HLDALPLPPAHAPEQPYRMLVAGMGGTGVITIGAIVSVAAHLQGLSASVLDLTGLAQKGG 766
HL+ + P + P ++ + ++++ V + A G +
Sbjct: 45 HLELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIEN 104

Query: 767 TVVSHIRLAP-PHAPEGPV--RLDWQQADAAILCDPVAAVAPDSLGALRRGHTQVVVNTY 823
++V I + +G V +L A+A L +++ L R +
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTL-KTQSSLLQARLEQTRYQILSRSIELN 163

Query: 824 VAPVSEFTRNPDAALRPEALLAKIRHAAGEARTAALDAHQAALALFGDSILSNMFMLGYA 883
P + P E + ++ Q
Sbjct: 164 KLPELKLPDEPYFQNVSEEEVLRLTSLI----KEQFSTWQNQKY---------------- 203

Query: 884 WQRGAVPLSHAALARAIELNGVAVQANRDAFEAGRLAAHQPQALE----DALRP--AAQV 937
ELN +A R A +E D Q
Sbjct: 204 ---------------QKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQA 248

Query: 938 VQLHVPESFERAVARRERDLTAYQN--AAYARQYRELVDRVAQREREL 983
+ H E +L Y++ + + +
Sbjct: 249 IAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLF 296


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0587TCRTETA651e-13 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 64.8 bits (158), Expect = 1e-13
Identities = 76/321 (23%), Positives = 113/321 (35%), Gaps = 16/321 (4%)

Query: 10 NRAIALLALAAFVSASAFRICDPMLPRLAADFGTSTGQAAATVTAFAVAYGLLQMFFGPV 69
NR + ++ + A + P+LP L D + A Y L+Q PV
Sbjct: 4 NRPLIVILSTVALDAVGIGLIMPVLPGLLRDL-VHSNDVTAHYGILLALYALMQFACAPV 62

Query: 70 G----DRYGKYRVVAVATFACAIGSMGAVLAPSLDVLVVCRALSGAAGAGIVPLSMAWIG 125
DR+G+ V+ V+ A+ AP L VL + R ++G GA ++ A+I
Sbjct: 63 LGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATG-AVAGAYIA 121

Query: 126 DNVPYEQRQATLARFLTGTILGMAAGQLAGGWFADTVGWRWAF---GALVAGYLIVGLLL 182
D ++R GM AG + GG F AL + G L
Sbjct: 122 DITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFL 180

Query: 183 WREVAQQAAASAARPAPGPRQGFAAQVRVVLGVPWARVVLATVFVEGLLVFGALAFAPAY 242
E + R A P F R + V ++A F+ L+ A +
Sbjct: 181 LPESHKGERRPLRREALNPLASFRW-ARGMTVVAA---LMAVFFIMQLVGQVPAALWVIF 236

Query: 243 LHARFGLSLTAAGAVVAVY-ALGGLLYTLVAGPVLRRLGERGLAAAG-GLVLCSAFLIYL 300
RF T G +A + L L ++ GPV RLGER G L+
Sbjct: 237 GEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAF 296

Query: 301 LGPAWGWGLAASMLAGFGYYL 321
W +LA G +
Sbjct: 297 ATRGWMAFPIMVLLASGGIGM 317


8Bpet0633Bpet0643Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0633013-4.083136AcrB/AcrD/AcrF family protein
Bpet0634122-4.990720DNA polymerase III subunit epsilon
Bpet0635223-4.213817*putative lipoprotein
Bpet0636018-2.010930hypothetical protein
Bpet06370121.243722hypothetical protein
Bpet06381132.152949major capsid protein precursor
Bpet06391122.667625hypothetical protein
Bpet06402122.939267putative acetyltransferase
Bpet06412113.202594putative bactoprenol glycosyltransferase
Bpet06421103.739677undecaprenolphosphate-sugar glycosyltransferase
Bpet06431103.589291hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0633ACRIFLAVINRP8630.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 863 bits (2231), Expect = 0.0
Identities = 327/1033 (31%), Positives = 555/1033 (53%), Gaps = 33/1033 (3%)

Query: 3 LSEICIKRPVFASVLSLIIVLVGLISYSRLTVREYPNIDEPIVSVNTIYKGASPEVIESQ 62
++ I+RP+FA VL++I+++ G ++ +L V +YP I P VSV+ Y GA + ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VTKPLEDQLAGIEGVNVMTSRS-RTERSQINIKFDLSRDPDAAAAEVRDKVSRARRYLPD 121
VT+ +E + GI+ + M+S S I + F DPD A +V++K+ A LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 EIDEPIIGKVEADAYPIIYIAVES--GSLSAIQTSDYINRYIKTRLSVLPGAAEVRVYGE 179
E+ + I ++ + ++ S + SDY+ +K LS L G +V+++G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 180 RLPSMRIYVDRDKLAAYNLTVQDVETALASQNVEIPAGRI------ESRDREFSVVSSTD 233
+ +MRI++D D L Y LT DV L QN +I AG++ + S+++ T
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 234 LQTPAQFAAIVV-ANVKGYPVRLGDVAKVEIGAADDRILSRFNGRPAINIGLTRQSTANP 292
+ P +F + + N G VRL DVA+VE+G + +++R NG+PA +G+ + AN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 293 LDLSKAVRAEVAQLNETLPAGMKLNIAYDSSVFIERSIQSVFRTIGEAIVLVVLVIFFFL 352
LD +KA++A++A+L P GMK+ YD++ F++ SI V +T+ EAI+LV LV++ FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 353 RNLRASIIPIVTIPVSLIGACGLMYLFGFSVNTLTLLAMVLAIGLVVDDAIVVLENIFRH 412
+N+RA++IP + +PV L+G ++ FG+S+NTLT+ MVLAIGL+VDDAIVV+EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 413 I-EEGMPRRQAAFQGAKEIGFAVVAMTLTLVTVYAPLAFATGRTGRLFIEFALTLAGAVL 471
+ E+ +P ++A + +I A+V + + L V+ P+AF G TG ++ +F++T+ A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 472 VSGFVALTLTPMMCSVLLR-----HEPRHNRWYNLVEGWLEALARGYRRALGLALRHRGV 526
+S VAL LTP +C+ LL+ H ++ + Y ++G L G
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 527 VVVVGLLVAGASGVLFSVVKSELAPIEDRGVIFGTVSAPEGATLNYTLESMLEIEHFYSQ 586
+++ L+ VLF + S P ED+GV + P GAT T + + ++ +Y +
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 587 IPEAE------ANQVSVGYPTVSDATAILRLKPWEQRERK---QQEIARELQPKFAGLPG 637
+A N S + A + LKPWE+R + + + + +
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 638 VKAFPTNPP---SLGQSARSKPVEFVIMSQASYPELARMVNVFVDALRSYPG-LQNLDTD 693
P N P LG + E + + + L + N + +P L ++ +
Sbjct: 660 GFVIPFNMPAIVELGTATGFDF-ELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 694 LRLNTPELRVQVDRDKMADVGAGVDVVGRTLESMLGGRQVTRFKDEGEQYDVIVQVLPRD 753
+T + +++VD++K +G + + +T+ + LGG V F D G + VQ +
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 754 RANPADISGIYVRTRDGSMVQLDNLLSVHESVSPQSLNHFNRLRAVKVEAAVAPGYALGE 813
R P D+ +YVR+ +G MV + H L +N L +++++ APG + G+
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 814 VLEHMHEVARQVLPHTVVTDLDGQAREFRDSSGSIYLVFAMALAFIYLVMAAQFESWRNP 873
+ M +A + LP + D G + + R S + A++ ++L +AA +ESW P
Sbjct: 839 AMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIP 897

Query: 874 FIIMLSVPLSMTGALLALWASGGTLSIYSQIGLITLVGLITKHGILIVEFANQLRD-QGR 932
+ML VPL + G LLA +Y +GL+T +GL K+ ILIVEFA L + +G+
Sbjct: 898 VSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGK 957

Query: 933 ELVDAVVEASVLRLRPILMTTGAMVLGTLPLAVSHGAGAESRQQIGWVLVGGLMLGTLLT 992
+V+A + A +RLRPILMT+ A +LG LPLA+S+GAG+ ++ +G ++GG++ TLL
Sbjct: 958 GVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLA 1017

Query: 993 LFVVPVAYTLIAT 1005
+F VPV + +I
Sbjct: 1018 IFFVPVFFVVIRR 1030



Score = 77.2 bits (190), Expect = 2e-16
Identities = 64/369 (17%), Positives = 135/369 (36%), Gaps = 36/369 (9%)

Query: 668 PELARMVN-VFVDALRSYPGLQNLDTDLRLNTPE--LRVQVDRDKMADVGAGVDVVGRTL 724
+++ V D L G+ D++L + +R+ +D D + V L
Sbjct: 152 DDISDYVASNVKDTLSRLNGV----GDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQL 207

Query: 725 ES----MLGGRQVTRFKDEGEQYDVIVQVLPRDRANPADISGIYVRT-RDGSMVQLDNLL 779
+ + G+ G+Q + + R NP + + +R DGS+V+L ++
Sbjct: 208 KVQNDQIAAGQLGGTPALPGQQLNASIIAQTR-FKNPEEFGKVTLRVNSDGSVVRLKDVA 266

Query: 780 SVHESVSPQ-SLNHFNRLRAVKVEAAVAPG---YALGEVLEHMHEVARQVLPHTVVTDLD 835
V + N A + +A G + ++ + P +
Sbjct: 267 RVELGGENYNVIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGM----- 321

Query: 836 GQAREFRDSSGSI---------YLVFAMALAFIYLVMAAQFESWRNPFIIMLSVPLSMTG 886
+ D++ + L A+ L F LVM ++ R I ++VP+ + G
Sbjct: 322 -KVLYPYDTTPFVQLSIHEVVKTLFEAIMLVF--LVMYLFLQNMRATLIPTIAVPVVLLG 378

Query: 887 ALLALWASGGTLSIYSQIGLITLVGLITKHGILIVE-FANQLRDQGRELVDAVVEASVLR 945
L A G +++ + G++ +GL+ I++VE + + +A ++
Sbjct: 379 TFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQI 438

Query: 946 LRPILMTTGAMVLGTLPLAVSHGAGAESRQQIGWVLVGGLMLGTLLTLFVVPV-AYTLIA 1004
++ + +P+A G+ +Q +V + L L+ L + P TL+
Sbjct: 439 QGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLK 498

Query: 1005 TVRRKPGHA 1013
V +
Sbjct: 499 PVSAEHHEN 507


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0640SACTRNSFRASE342e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 33.8 bits (77), Expect = 2e-04
Identities = 13/56 (23%), Positives = 21/56 (37%), Gaps = 3/56 (5%)

Query: 90 VHDCAVLPAAQGLGVAQALLQGGLEHARRRGLCHTSLVALR---PAVSYWERLGYR 142
+ D AV + GV ALL +E A+ C L A ++ + +
Sbjct: 92 IEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI 147


9Bpet0713Bpet0725Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0713193.589608hypothetical protein
Bpet0714083.163463LysR family transcriptional regulator
Bpet0715093.015983putative hydrolase
Bpet0716093.150547acyl-CoA synthetase, long-chain-fatty-acid--CoA
Bpet0717-192.882398inositol monophosphatase-family protein
Bpet0718-192.786672hypothetical protein
Bpet0719-2121.103313putative glycosyl hydrolase
Bpet07200150.592733putative amino acid ABC transporter ATP-binding
Bpet07211150.859748ABC transport protein, inner membrane component
Bpet07221150.827442hypothetical protein
Bpet07232151.613354*hypothetical protein
Bpet07241151.786729putative molybdate-binding ABC-transporter
Bpet07252181.977398molybdenum transport system permease protein
10Bpet0736Bpet0758Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0736-2143.172566hypothetical protein
Bpet0737-1133.006159TetR family transcriptional regulator
Bpet07380133.660731putative short-chain dehydrogenase
Bpet0739-1143.158117IclR family transcriptional regulator
Bpet07401154.276185hypothetical protein
Bpet07410154.117696IclR family transcriptional regulator
Bpet0742-1143.706440putative secreted protein
Bpet0743-1133.824723putative D-isomer specific 2-hydroxyacid
Bpet0744-2142.131461hypothetical protein
Bpet07450123.594497putative aldolase
Bpet07460132.972682*putative lipoprotein
Bpet07470112.196839hypothetical protein
Bpet07481102.926709ornithine cyclodeaminase
Bpet07491122.857014AsnC family transcriptional regulator
Bpet07500113.371953putative integral membrane protein
Bpet0751-1132.434592LysR family transcriptional regulator
Bpet0752-2141.859200hypothetical protein
Bpet07530153.613140hypothetical protein
Bpet0754-2163.505584hypothetical protein
Bpet0755-1152.265006putative lipoprotein
Bpet0756-3152.606286pterin-4-alpha-carbinolamine dehydratase
Bpet0757-3152.870209hypothetical protein
Bpet0758-3153.721004putative nucleotidyl transferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0737HTHTETR682e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 68.1 bits (166), Expect = 2e-16
Identities = 37/178 (20%), Positives = 68/178 (38%), Gaps = 3/178 (1%)

Query: 1 MADRGRPRSFD-RDTALQKAMDLFWEKGYASTSLADLTAAMGINAPSLYSAFGSKEQLFR 59
MA + + + + R L A+ LF ++G +STSL ++ A G+ ++Y F K LF
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 60 DAVALYGSREGGCTQAELLSAP-TVRAGIENMLLAAVRAGTQPGRPKGCLIVLGAPT-GT 117
+ L S G P + + +L+ + + R + + ++
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 118 DEHAPVQKMLCDSRRHTQALILQRLREAVRHGELPAHTDLPALASYYATVLHGMAIQA 175
E A VQ+ + + I Q L+ + LPA A + G+
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENW 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0738DHBDHDRGNASE1321e-39 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 132 bits (332), Expect = 1e-39
Identities = 88/253 (34%), Positives = 136/253 (53%), Gaps = 15/253 (5%)

Query: 4 LQGKIAFVTGGSRGIGAAIARHLAARGADVAITYVSTPERAQELVAELRGAGRRAHAYAA 63
++GKIAF+TG ++GIG A+AR LA++GA +A PE+ +++V+ L+ R A A+ A
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPA 64

Query: 64 DAADHQQVRAAVEQAVRDLGGLDILVNNAGIFIAGGLDTLSHADFQRTLDVNVSAVFAAT 123
D D + + R++G +DILVN AG+ G + +LS +++ T VN + VF A+
Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNAS 124

Query: 124 QAALPHLP--RGGRIINIGSCLAGRAGDAGLAAYSASKAAVAGLTKGAARDLGPRGITVN 181
++ ++ R G I+ +GS AG +AAY++SKAA TK +L I N
Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAG-VPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 182 VVHPGPIDTDMNP---AQREGAAESAARLA--------LQRYGHVDDIAGMVGYLASPAA 230
+V PG +TDM A GA + L++ DIA V +L S A
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 231 GYVTGAEISVDGG 243
G++T + VDGG
Sbjct: 244 GHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0745PHPHTRNFRASE391e-05 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 39.4 bits (92), Expect = 1e-05
Identities = 24/103 (23%), Positives = 36/103 (34%), Gaps = 13/103 (12%)

Query: 155 AMIESRQGLWHADEIAAVEGIDMLLVGAGDL-----AADLGAAGPA-----VQAALRDAF 204
M+E A+ A + +D +G DL AAD + A+
Sbjct: 429 IMVEIPSTAVAANLFA--KEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPAILRLV 486

Query: 205 DTVIAACKRHGKAAGA-GGLAGQLDLLAEVVAAGVRYVSAGTD 246
D VI A GK G G +AG + ++ G+ S
Sbjct: 487 DMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSAT 529


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0750TCRTETB388e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 37.6 bits (87), Expect = 8e-05
Identities = 31/134 (23%), Positives = 58/134 (43%), Gaps = 11/134 (8%)

Query: 35 IAGPMLQSDLGLNLDALGWLTGIFAVLGVAGGIPAGVVIGSVGGRRALVGGLLATAVGAA 94
++ P + +D + W+ F + G G + +G +R L+ G++ G+
Sbjct: 35 VSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSV 94

Query: 95 VGAAS-PVYGWLLASRVIEGAGFLFITVAGPAVLQ--RQDMVRADRRDLAFALWSCFMPA 151
+G + L+ +R I+GAG A PA++ + + R AF L +
Sbjct: 95 IGFVGHSFFSLLIMARFIQGAG----AAAFPALVMVVVARYIPKENRGKAFGLIG----S 146

Query: 152 GMAIAMLVGPLLGG 165
+A+ VGP +GG
Sbjct: 147 IVAMGEGVGPAIGG 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0755OMPADOMAIN902e-23 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 89.6 bits (222), Expect = 2e-23
Identities = 40/126 (31%), Positives = 63/126 (50%), Gaps = 11/126 (8%)

Query: 101 KVNIPSNVSFDTDKAVLKPALLPVLDSVARALNQH--PELRAKVVGHTDSTGSAAHNQTL 158
+ S+V F+ +KA LKP LD + L+ + V+G+TD GS A+NQ L
Sbjct: 214 HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGL 273

Query: 159 SENRARSVTDYLGKQGVAPARMTIEGRAARDPIGDNATAEGR---------AANRRVEVY 209
SE RA+SV DYL +G+ +++ G +P+ N + A +RRVE+
Sbjct: 274 SERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIE 333

Query: 210 LYAVKQ 215
+ +K
Sbjct: 334 VKGIKD 339


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0757GPOSANCHOR300.008 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 30.0 bits (67), Expect = 0.008
Identities = 13/60 (21%), Positives = 25/60 (41%)

Query: 59 EQSEQLQSELNTANLDRQRLRTQLAEAVAQRDANQATRDQASADLEQARARIQALNQEFK 118
E +QL++E + DA++ + Q LE+A +++ AL + K
Sbjct: 358 EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNK 417



Score = 28.9 bits (64), Expect = 0.017
Identities = 15/74 (20%), Positives = 27/74 (36%), Gaps = 14/74 (18%)

Query: 59 EQSEQLQSELNTANLDRQRLRTQLAEAVA--------------QRDANQATRDQASADLE 104
+ L+ + N +RQ LR L + Q ++A+R DL+
Sbjct: 295 AEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLD 354

Query: 105 QARARIQALNQEFK 118
+R + L E +
Sbjct: 355 ASREAKKQLEAEHQ 368


11Bpet0773Bpet0778Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0773-1223.817916tricarballylate dehydrogenase
Bpet0774-1233.656818LysR family transcriptional regulator
Bpet07750203.628935hypothetical protein
Bpet0776-1203.5677811-acyl-sn-glycerol-3-phosphate acyltransferase
Bpet0777-2184.584377D,D-heptose 1,7-bisphosphate phosphatase
Bpet0778-1184.214179glycyl-tRNA synthetase beta chain
12Bpet0813Bpet0855Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0813-1143.254148hypothetical protein
Bpet0814-2152.989680putative secreted protein
Bpet0815-1163.464067putative acyl-CoA dehydrogenase
Bpet0816-2133.912368putative acyl-CoA dehydrogenase
Bpet0817-1152.919048enoyl-CoA hydratase
Bpet0818-1152.441780hypothetical protein
Bpet08190162.503748hypothetical protein
Bpet0820-1162.985039putative citrate lyase beta chain
Bpet08210163.5090042-amino-4-hydroxy-6-
Bpet08220163.932974poly(A) polymerase
Bpet08230163.887046hypothetical protein
Bpet08240153.946421DnaA regulatory inactivator Hda
Bpet0825-1143.022679phosphoribosylaminoimidazole synthetase
Bpet0826-2122.088726tRNA delta(2)-isopentenylpyrophosphate
Bpet0827-2121.018506DNA mismatch repair protein
Bpet0828-2110.286242hypothetical protein
Bpet0829-2100.301553hypothetical protein
Bpet0830-2120.796002hypothetical protein
Bpet0831-1121.774407hypothetical protein
Bpet0832-1162.915136fumarate hydratase
Bpet0833-2163.368766hypothetical protein
Bpet0834-2183.552900putative secreted protein
Bpet0835-1163.846613LysR family transcriptional regulator
Bpet0836-1164.253494hypothetical protein
Bpet0837-1153.920037hypothetical protein
Bpet0838-1153.377100putative secreted protein
Bpet0839-1132.878887acyl-CoA dehydrogenase
Bpet0840-1131.522600LysR family transcriptional regulator
Bpet0841-2133.631217hypothetical protein
Bpet0842-2142.288166putative lipoprotein
Bpet0843-2142.232551hypothetical protein
Bpet08441162.028789hypothetical protein
Bpet08451172.996305putative C4-dicarboxylate transport system,
Bpet08462162.617706putative amidase
Bpet08470161.104108putative C4-dicarboxylate transport system,
Bpet08481171.525800hypothetical protein
Bpet08492172.269439hypothetical protein
Bpet08500163.354438hypothetical protein
Bpet08511163.114053acyl-CoA dehydrogenase
Bpet08521183.558956hypothetical protein
Bpet08532184.197162GntR family transcriptional regulator
Bpet08540174.402187MFS permease
Bpet0855-3173.725653putative citrate lyase beta chain
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0820ECOLIPORIN290.014 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 29.5 bits (66), Expect = 0.014
Identities = 22/81 (27%), Positives = 31/81 (38%), Gaps = 14/81 (17%)

Query: 126 NAAEIAATPGVQRLAF--------GSLDYGLDLGLTTDSDGAGVVL------DHARVQVL 171
N E RLAF GS DYG + G+ D +G +L +
Sbjct: 84 NTTEGEGANSWTRLAFAGLKFGDYGSFDYGRNYGVLYDVEGWTDMLPEFGGDSYTYADNY 143

Query: 172 LRSRAAGLAPALDGVFPGVQD 192
+ RA G+A + F G+ D
Sbjct: 144 MTGRANGVATYRNTDFFGLVD 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0837BORPETOXINA310.008 Bordetella pertussis toxin A subunit signature.
		>BORPETOXINA#Bordetella pertussis toxin A subunit signature.

Length = 269

Score = 30.9 bits (69), Expect = 0.008
Identities = 23/91 (25%), Positives = 40/91 (43%), Gaps = 1/91 (1%)

Query: 273 ADDARFASNASRLQHREALRDELAALLAGHDAAALAEQLLRQGVPAAAVQNVEDVLHHPH 332
AD+ + + +S ++ + D +LAG A +E L + +P ++ V V H+
Sbjct: 127 ADNNFYGAASSYFEYVDTYGDNAGRILAGALATYQSEYLAHRRIPPENIRRVTRVYHNGI 186

Query: 333 TRHRGMVLEHGNYRGVGSPIKLSRTPATLRR 363
T E+ N R V + + P T RR
Sbjct: 187 TGET-TTTEYSNARYVSQQTRANPNPYTSRR 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0842PF03544300.007 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 30.3 bits (68), Expect = 0.007
Identities = 12/42 (28%), Positives = 15/42 (35%)

Query: 254 AAARPGTGAPAAPTRPPAPPAAAPAAQPAPQPAPQPAPATPR 295
P P P PP AP P+P P+P P +
Sbjct: 68 PPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVK 109


13Bpet0864Bpet0881Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0864-1133.305221hypothetical protein
Bpet0865-1133.434921putative inner membrane efflux protein
Bpet0866-2134.133604hypothetical protein
Bpet0867-1144.521882GntR family transcriptional regulator
Bpet0868-2173.672003acyl-CoA synthetase
Bpet0869-1173.648457putative secreted protein
Bpet0870-1163.744828short-chain dehydrogenase
Bpet0871-1174.010763hypothetical protein
Bpet0872-2163.503028aminodeoxychorismate synthase
Bpet0873-2152.752394putative excinuclease ABC subunit
Bpet0874-1123.130173IclR family transcriptional regulator
Bpet08750132.743654KHG/KDPG aldolase
Bpet0876-4131.142447putative gluconate kinase
Bpet0877-4110.117598LysR family transcriptional regulator
Bpet0878-4120.244075putative glycosyltransferase
Bpet0879-113-0.065821hypothetical protein
Bpet08800130.427542hypothetical protein
Bpet08812110.803279putative ABC transporter ATP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0865TCRTETA501e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 49.8 bits (119), Expect = 1e-08
Identities = 66/321 (20%), Positives = 114/321 (35%), Gaps = 10/321 (3%)

Query: 86 GGLVAVFAVIPMLMSVRAGQWIDRVGVRRPVTLGNGLVVTGTVLPFAF-QTQWALLVAAC 144
G L+A++A++ + G DR G RRPV L + A W L +
Sbjct: 46 GILLALYALMQFACAPVLGALSDRFG-RRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRI 104

Query: 145 SIGVGFMLHQVSTQDVLGHAEPQRRLRNFSWLSLAMAGSGFSGPLIAGLAIDHLGSRMAF 204
G+ V+ + + R R+F ++S +GP++ GL F
Sbjct: 105 VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPF 163

Query: 205 GILA-LGPLVALAGLRRLQPALRAMDHAIEPTERQARRR-PVAELLKLPPLRRILMVNTI 262
A L L L G L + + + A + + +
Sbjct: 164 FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQ 223

Query: 263 LSGAWDTHLFVVPLFGV-AIGLSATTIGSILAAF-ALGTFLIRLVLPFIQTRVRSWTLIR 320
L G L+V +FG ATTIG LAAF L + ++ + R+ +
Sbjct: 224 LVGQVPAALWV--IFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM 281

Query: 321 TAMGITAADFLIYPLFAEVTTLIGLSFILGLALGCCQPSILSLLHQHTPHGRAAEAVGLR 380
M +++ FA + +L + G P++ ++L + R + G
Sbjct: 282 LGMIADGTGYILL-AFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL 340

Query: 381 MAFINASQVSLPLTFGALGAV 401
A + + + PL F A+ A
Sbjct: 341 AALTSLTSIVGPLLFTAIYAA 361


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0870DHBDHDRGNASE1191e-34 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 119 bits (298), Expect = 1e-34
Identities = 68/250 (27%), Positives = 109/250 (43%), Gaps = 18/250 (7%)

Query: 6 ALITGASRGIGRAIAVRLIEDGYDVVNFSRGAPAALLAGETF---------VSVDLADAA 56
A ITGA++GIG A+A L G + + D+ D+A
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70

Query: 57 RTSQAVAELAAQREVL-YLVNNAGMIKVADIERVSGQAMQETLAVNLVAPLLLLQGLLPS 115
+ A + + + LVN AG+++ I +S + + T +VN + +
Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130

Query: 116 MRRRGHGRVVNIGSRAA-LGKPGRTAYGASKAGLAGMTRTWALELAASGITVNAVAPGPV 174
M R G +V +GS A + + AY +SKA T+ LELA I N V+PG
Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGST 190

Query: 175 ATELFNQSNPPDHPRTRELAAS-------IPVGRIGQPEDVAHAVAMLLDPRAGFITGQT 227
T++ ++ + + S IP+ ++ +P D+A AV L+ +AG IT
Sbjct: 191 ETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITMHN 250

Query: 228 LYVCGGMTVG 237
L V GG T+G
Sbjct: 251 LCVDGGATLG 260


14Bpet0893Bpet0909Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0893-2184.012721septum formation inhibitor
Bpet0894-2153.744851glutaminyl-tRNA synthetase
Bpet0895-1164.154656putative transmembrane cytochrome oxidase
Bpet0896-3163.529672putative cytochrome oxidase
Bpet0897-2163.729590two-component sensor kinase
Bpet0898-2153.376808transcriptional regulator
Bpet0899-2132.911622putative secreted protein
Bpet0900-2162.824170DNA helicase
Bpet0901-2203.156559Holliday junction DNA helicase RuvB
Bpet0902-1183.163349serine/threonine dehydratase
Bpet0903-1173.181974NADH dehydrogenase
Bpet09040184.344160Holliday junction DNA helicase RuvA
Bpet09050194.451096Holliday junction resolvase
Bpet09060184.299607bifunctional
Bpet09070153.699916DNA-binding protein fis
Bpet09080133.441489dihydrouridine synthase
Bpet09091123.398144hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0893TONBPROTEIN300.012 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 29.6 bits (66), Expect = 0.012
Identities = 26/110 (23%), Positives = 31/110 (28%), Gaps = 5/110 (4%)

Query: 103 PPARPAPAVETAPPNDAATPVPAVPAAALETGASATTGNAPAEPAPAEPAAPAAAPQPPA 162
P V P P P E +P E APA A
Sbjct: 79 PEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTA 138

Query: 163 VPAPA----SALVITKPLRSGQRVY-ARHTDLVVIGMVSQGAEVIADGNV 207
A + S + L Q Y AR L + G V +V DG V
Sbjct: 139 TAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRV 188


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0897PF06580300.021 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.2 bits (68), Expect = 0.021
Identities = 17/107 (15%), Positives = 35/107 (32%), Gaps = 25/107 (23%)

Query: 392 LLDNLIGNALHHGEPP------VDVSLRREGGMAMLDVADHGRGIAPERRSEALRPFARL 445
L+ L+ N + HG + + ++ G L+V + G +
Sbjct: 259 LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKEST------- 311

Query: 446 DDARTRTGNVGLGLA-LAEAIARAHGGQLAL-LQADSGGLLVRITLP 490
G GL + E + +G + + L G + + +P
Sbjct: 312 ----------GTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0898HTHFIS1021e-27 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 102 bits (257), Expect = 1e-27
Identities = 52/148 (35%), Positives = 79/148 (53%), Gaps = 1/148 (0%)

Query: 6 TKLLVVDDDPALRQLLADYLNRHGYDTLLAPDANDLAARIARYAPDLLVLDRMLPGGDGA 65
+LV DDD A+R +L L+R GYD + +A L IA DL+V D ++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 DACRRLREQGEDIPVILLTARDEAVDRIIGLEAGADDYLGKPFDPRELLARIE-AVLRRK 124
D R+++ D+PV++++A++ + I E GA DYL KPFD EL+ I A+ K
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 125 RGPSALTKDAPVSFGPFVFDPAMRQLLR 152
R PS L D+ AM+++ R
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYR 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0899PF00577563e-10 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 55.6 bits (134), Expect = 3e-10
Identities = 36/236 (15%), Positives = 62/236 (26%), Gaps = 42/236 (17%)

Query: 268 GRLAYSSTVGVLNYTDMAARSGAIDYGVTAGSGTLRYGLTPELTLESQMQSAPDLSTRGL 327
G YS T G A TL +GL T+ Q A
Sbjct: 372 GHTRYSITAGEYR------SGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNF 425

Query: 328 GSTYSAGDLGTFQAGATQSSFD-----DINAWRYRFGYNVNLFE---SVSLAVTNEQIGA 379
G + G LG TQ++ + RF YN +L E ++ L +
Sbjct: 426 GIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLV-GYRYSTS 484

Query: 380 GFGDLAQY-------------------------RNGVAAAPQMRNTLAAGVPIMGWGTLT 414
G+ + A +A + + L + TL
Sbjct: 485 GYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLY 544

Query: 415 GTYSGLRQSGEPIEQR-FGLQHSMLIA-PSVRLAVGADRDVVTGDYEMRAGVTMPV 468
+ S G F + + L+ ++ + + + +
Sbjct: 545 LSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0900HTHFIS310.018 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.018
Identities = 17/78 (21%), Positives = 32/78 (41%), Gaps = 4/78 (5%)

Query: 158 LSMLHERWPDVPRIALTATATAATRVEIAQRLALDQARHFVASFDRPNIRYRIV-EKNEV 216
L + + PD+P + ++A T T ++ +++ A D FD + I E
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDY---LPKPFDLTELIGIIGRALAEP 122

Query: 217 RRQLLDLIRAEHEGDSGV 234
+R+ L +G V
Sbjct: 123 KRRPSKLEDDSQDGMPLV 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0903NUCEPIMERASE512e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 51.3 bits (123), Expect = 2e-09
Identities = 27/132 (20%), Positives = 47/132 (35%), Gaps = 24/132 (18%)

Query: 1 MRILLIGGTGFLGRHMAARLAGHGHVLIV---------PTRQYGRGRDLQLL--PTLTLV 49
M+ L+ G GF+G H++ RL GH ++ + + R L+LL P
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQAR---LELLAQPGFQFH 57

Query: 50 EADVHDDAVLDRLLR--ECDAVINLAGILHGGRGQPYGAGFARVHVQLP----QRIAQAC 103
+ D+ D + L + V Y + I + C
Sbjct: 58 KIDLADREGMTDLFASGHFERVFISP----HRLAVRYSLENPHAYADSNLTGFLNILEGC 113

Query: 104 RRHGVRRLLHVS 115
R + ++ LL+ S
Sbjct: 114 RHNKIQHLLYAS 125


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0907DNABINDNGFIS684e-19 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 68.5 bits (167), Expect = 4e-19
Identities = 29/74 (39%), Positives = 52/74 (70%)

Query: 4 KDVLEDCVRASLERYFEDLGESEPHDMWDMVMRCVERPVLEVALQRSGGNQSRASEMLGI 63
+ L D V+ +L+ YF L + +D++++V+ VE+P+L++ +Q + GNQ+RA+ M+GI
Sbjct: 24 QKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGI 83

Query: 64 TRNTLRKKLQAHNI 77
R TLRKKL+ + +
Sbjct: 84 NRGTLRKKLKKYGM 97


15Bpet0952Bpet0963Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet09522170.917006phage-related DNA recombination protein
Bpet09530190.722575hypothetical protein
Bpet0954-322-1.024653hypothetical protein
Bpet0955-226-3.130504hypothetical protein
Bpet0956-225-4.429284hypothetical protein
Bpet0957-228-6.311196hypothetical protein
Bpet0958-231-8.183497hypothetical protein
Bpet0959035-8.422813hypothetical protein
Bpet0960038-8.411311hypothetical protein
Bpet0961-226-5.483737hypothetical protein
Bpet0962-120-4.133661hypothetical protein
Bpet0963022-3.892939putative transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0961TYPE3IMSPROT270.016 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 26.6 bits (59), Expect = 0.016
Identities = 11/55 (20%), Positives = 19/55 (34%)

Query: 17 KSVGVAYLLWFFLGGVGGHRFYAGKTGSAIAIIALTIIGVLLSVVGVGFFLLFIV 71
K V ++ L+W + G G L I L V+ F++ +
Sbjct: 146 KVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQILRQLMVICTVGFVVISI 200


16Bpet0973Bpet1146Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet09732160.246469hypothetical protein
Bpet09742170.555504hypothetical protein
Bpet09752181.127705hypothetical protein
Bpet09763191.415367hypothetical protein
Bpet09774201.752908hypothetical protein
Bpet09783221.940894hypothetical protein
Bpet09791221.363770hypothetical protein
Bpet09801211.463423hypothetical protein
Bpet09812211.314308hypothetical protein
Bpet09821190.654207hypothetical protein
Bpet09832170.369571hypothetical protein
Bpet0984317-0.038123hypothetical protein
Bpet0985114-0.055468hypothetical protein
Bpet0986114-0.257363putative secreted protein
Bpet0987014-0.570234hypothetical protein
Bpet09881150.071476hypothetical protein
Bpet09891150.125877hypothetical protein
Bpet09902160.132884bacteriophage-related transmembrane protein
Bpet09913170.103586hypothetical protein
Bpet0992215-0.602819hypothetical protein
Bpet09932140.072357hypothetical protein
Bpet0994214-0.216368putative bacteriophage protein
Bpet0995219-1.144832hypothetical protein
Bpet0996118-1.465824hypothetical protein
Bpet0997120-2.153889hypothetical protein
Bpet0998020-1.533569hypothetical protein
Bpet0999-124-3.314964hypothetical protein
Bpet1000335-7.993557tail fiber protein, putative
Bpet1001233-8.122832putative phage tail fibre protein
Bpet1002330-6.852734hypothetical protein
Bpet1003434-7.949375phage related lysozyme
Bpet1004433-8.145331hypothetical protein
Bpet1005434-7.749551hypothetical protein
Bpet1006225-3.337121centaurin, alpha
Bpet1007227-2.818806hypothetical protein
Bpet1008324-2.504116hypothetical protein
Bpet1009621-0.524681*putative NADPH-dependent FMN reductase
Bpet1010724-0.333796hypothetical protein
Bpet1011621-0.109254MerR family transcriptional regulator
Bpet1012225-2.154045hypothetical protein
Bpet1013226-2.806274ParB-like nuclease
Bpet1014328-3.677557transposase insF
Bpet1015231-4.679707IstB-like ATP-binding protein
Bpet1016030-4.356700hypothetical protein
Bpet1017027-4.561849hypothetical protein
Bpet1018318-2.658190integrase catalytic subunit
Bpet1019315-1.991585transposase IS911 HTH and LZ region
Bpet1020316-1.860590MerR family transcriptional regulator
Bpet1021317-1.232211putative transposase
Bpet1022317-2.138079arsenate reductase
Bpet1023418-1.962949putative sodium bile acid symporter family
Bpet1024220-1.441463hypothetical protein
Bpet1025322-0.889152glyoxalase family protein
Bpet10263211.094722ArsR family transcriptional regulator
Bpet10274211.028563receptor protein-tyrosine kinase
Bpet10283192.525004putative transcriptional regulator
Bpet10293182.364015hypothetical protein
Bpet10303172.124192hypothetical protein
Bpet10313171.807307hypothetical protein
Bpet10324160.933855hypothetical protein
Bpet10332161.323498hypothetical protein
Bpet1034217-1.503198putative transposon
Bpet1035221-3.658165hypothetical protein
Bpet1036326-5.657426single-stranded DNA-binding protein
Bpet1037226-4.678316DNA topoisomerase III
Bpet1038231-6.699300putative DNA-cytosine methyltransferase
Bpet1039335-7.531262tyrosine recombinase xerD
Bpet1040232-6.248755integrase/recombinase
Bpet1041230-4.278428integrase/recombinase
Bpet1042327-0.978942putative DNA-cytosine methyltransferase
Bpet1043628-2.399530hypothetical protein
Bpet1044628-2.686539hypothetical protein
Bpet1045724-2.706333hypothetical protein
Bpet1046723-2.521080hypothetical protein
Bpet1047823-1.955721hypothetical protein
Bpet1048924-1.937367hypothetical protein
Bpet1049822-1.634895hypothetical protein
Bpet1050823-1.523504hypothetical protein
Bpet1051823-1.299588hypothetical protein
Bpet1052626-4.656511hypothetical protein
Bpet1053527-5.649594hypothetical protein
Bpet1054427-5.457518hypothetical protein
Bpet1055532-8.046183hypothetical protein
Bpet1056529-6.970293hypothetical protein
Bpet1057529-6.995106reverse transcriptase
Bpet1058525-5.245074hypothetical protein
Bpet1059421-3.923028hypothetical protein
Bpet1060521-4.166672reverse transcriptase
Bpet1061417-1.444162hypothetical protein
Bpet1062318-0.958441hypothetical protein
Bpet10632160.115732hypothetical protein
Bpet10643170.943914hypothetical protein
Bpet10651173.536351hypothetical protein
Bpet10661182.922023putative secreted protein
Bpet10672192.622759hypothetical protein
Bpet10682192.585515hypothetical protein
Bpet10692201.999008hypothetical protein
Bpet10702201.287930hypothetical protein
Bpet10713200.521798hypothetical protein
Bpet10725181.248469hypothetical protein
Bpet10734202.216831hypothetical protein
Bpet10744172.021788hypothetical protein
Bpet10754172.165502hypothetical protein
Bpet10764161.376713hypothetical protein
Bpet10773151.258210hypothetical protein
Bpet10783150.914178hypothetical protein
Bpet10793160.575222hypothetical protein
Bpet1080419-0.121331putative lipoprotein
Bpet10814190.061121hypothetical protein
Bpet10824220.546511hypothetical protein
Bpet1083423-0.827252DNA repair protein radC-like protein
Bpet1084323-0.786506putative secreted protein
Bpet1085422-1.021483hypothetical protein
Bpet1086422-0.898973hypothetical protein
Bpet1087318-1.595546hypothetical protein
Bpet1088419-2.570298hypothetical protein
Bpet1089320-2.542309hypothetical protein
Bpet1090320-2.683280hypothetical protein
Bpet1091129-5.040826hypothetical protein
Bpet1092135-6.547206putative helicase
Bpet1093149-10.665845hypothetical protein
Bpet1094-137-7.184582transposase
Bpet1095137-7.408988TRm3 transposase
Bpet1097238-7.955862putative secreted protein
Bpet1098133-6.814529hypothetical protein
Bpet1099132-6.212091taurine dioxygenase
Bpet1100229-4.128401transposase
Bpet1101434-6.266072Alpha-ketoglutarate-dependent taurine
Bpet1102338-6.446784hypothetical protein
Bpet1103335-5.493464hypothetical protein
Bpet1104328-4.356290Hydantoin utilization protein A
Bpet1105134-5.279203putative transposase
Bpet1106129-3.595633putative transposase
Bpet1107138-4.537621hypothetical protein
Bpet1108133-3.752828integrase catalytic subunit
Bpet1109-132-3.350821transposase IS911 HTH and LZ region
Bpet1110033-3.258964hypothetical protein
Bpet1111031-3.001425transposase
Bpet1112135-4.784980N-methylhydantoinase A
Bpet1113129-4.051564putative transposase
Bpet1114230-4.449281putative transposase
Bpet1115231-4.978215hypothetical protein
Bpet1116132-4.989264acyl-CoA dehydrogenase
Bpet1117129-4.740523metallo-beta-lactamase family protein
Bpet1118028-4.329259putative phenylacetate-CoA ligase
Bpet1119026-4.618441enoyl-CoA hydratase
Bpet1120024-4.482281hypothetical protein
Bpet1121-123-4.248553putative thiolase
Bpet1122-122-4.514787putative ligase
Bpet1123-122-4.1976043-hydroxyacyl-CoA dehydrogenase
Bpet1124022-4.746643putative high-affinity branched-chain amino acid
Bpetpseudo_02-120-4.752757hypothetical protein
Bpet1125018-4.989017transposase
Bpet1126016-5.084965transposase
Bpetpseudo_03016-4.646222hypothetical protein
Bpet1127-118-4.451637branched-chain amino acid transport system
Bpet1128-219-4.378891high-affinity branched-chain amino acid
Bpet1129-122-5.485827ABC-type branched-chain amino acid transport
Bpet1130-127-5.124745putative branched-chain amino acid ABC
Bpet1131031-5.400418putative enoyl-CoA hydratase/isomerase
Bpet1132135-6.5272792-deoxy-D-gluconate 3-dehydrogenase
Bpet1133034-5.784943hypothetical protein
Bpet1134031-5.006942TetR family transcriptional regulator
Bpet1135-129-2.877413hypothetical protein
Bpet1136-130-2.950136hypothetical protein
Bpet1137127-2.509579hypothetical protein
Bpet1138324-1.265129peptidoglycan binding-like domain-containing
Bpet1139324-1.591692putative transposase
Bpet1140426-1.493044hypothetical protein
Bpet1141429-2.941986hypothetical protein
Bpet1142527-3.014616ISSfl3 orfA
Bpet1143528-3.083120hypothetical protein
Bpet1144529-3.562943hypothetical protein
Bpet1145528-3.385025hypothetical protein
Bpet1146225-3.355645insertion sequence IS5376 putative ATP-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0990PREPILNPTASE300.033 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 29.8 bits (67), Expect = 0.033
Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 4/50 (8%)

Query: 319 ALAALSYGIVRVTRLLRTMRVAAWASLGPYLAIAAALAAIYLVGQDIWVW 368
+L GI LLR + GPYLAIA +A ++ G I W
Sbjct: 239 SLVGAFMGIGL--ILLRNHHQSKPIPFGPYLAIAGWIALLW--GDSITRW 284


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1004AEROLYSIN260.018 Aerolysin signature.
		>AEROLYSIN#Aerolysin signature.

Length = 493

Score = 26.2 bits (57), Expect = 0.018
Identities = 12/36 (33%), Positives = 16/36 (44%)

Query: 54 RREAQEVRNETAAMADDAIAAELADNWVRKPAGKGG 89
R EAQ V++ M + LA+ WV G G
Sbjct: 51 REEAQSVKSNIVGMMGQWQISGLANGWVIMGPGYNG 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1023ACRIFLAVINRP290.033 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.0 bits (65), Expect = 0.033
Identities = 12/66 (18%), Positives = 25/66 (37%), Gaps = 3/66 (4%)

Query: 203 IVIPVILAQLWRRALLSKGQAAFDRALERIGPL---SIAALLLTLVLLFAFQGEAIIRQP 259
I+I L + +A R+ P+ S+A +L L L + + +
Sbjct: 942 ILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNA 1001

Query: 260 LVIAML 265
+ I ++
Sbjct: 1002 VGIGVM 1007


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1031ARGREPRESSOR290.040 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 28.7 bits (64), Expect = 0.040
Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 12/46 (26%)

Query: 168 SQSELARRLAADGFPVRRSHITRMAD---AVR---------YLLPA 201
+Q EL L DG+ V ++ ++R V+ Y LPA
Sbjct: 21 TQDELVDILKKDGYNVTQATVSRDIKELHLVKVPTNNGSYKYSLPA 66


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1089OMPADOMAIN260.043 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 26.0 bits (57), Expect = 0.043
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 5/34 (14%)

Query: 14 GRTFGRGWRAY--ARGERWAS---NWLVSKGVPA 42
G T G AY ER A ++L+SKG+PA
Sbjct: 259 GYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPA 292


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1123DHBDHDRGNASE998e-27 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 98.6 bits (245), Expect = 8e-27
Identities = 61/199 (30%), Positives = 86/199 (43%), Gaps = 16/199 (8%)

Query: 3 VNNKVAVVTGAASGLGLATCKALAAAGARVVGFD------LDAKTVQAALAPDIRGLAVD 56
+ K+A +TGAA G+G A + LA+ GA + D + A A D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 57 VANEADIKTGIDTVLELHGAIHIVVNCAGILGPCKTLSKGQMFPTELWERVIAVNLSGTF 116
V + A I + G I I+VN AG+L P S E WE +VN +G F
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHS----LSDEEWEATFSVNSTGVF 121

Query: 117 NMIRHAALAMSRNEPDESGDRGVIVNTASGAAWQGQMGQAAYSASKAGVIGMTLPIARDL 176
N R + M G IV S A + AAY++SKA + T + +L
Sbjct: 122 NASRSVSKYMMDRR------SGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLEL 175

Query: 177 AEHGIRMVAIAPGLFDTGM 195
AE+ IR ++PG +T M
Sbjct: 176 AEYNIRCNIVSPGSTETDM 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1126HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1132DHBDHDRGNASE1283e-38 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 128 bits (322), Expect = 3e-38
Identities = 80/259 (30%), Positives = 123/259 (47%), Gaps = 12/259 (4%)

Query: 4 NLFSLHGKTALITGASSGIGQHVAGVFARAGATVVLAARRMDRIEAAVAALREQGHAAHG 63
N + GK A ITGA+ GIG+ VA A GA + +++E V++L+ + A
Sbjct: 2 NAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEA 61

Query: 64 IYLDVTRTETIAAAFDRAEQQCGAPIDILYNNSGVIHVSPFVEQKEEEIARIFDTNLKGA 123
DV + I R E++ G PIDIL N +GV+ +EE F N G
Sbjct: 62 FPADVRDSAAIDEITARIEREMG-PIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 124 MLVAQEAARRMLPQRAGAIVNIASVAGMRAGGWLASYAASKAALIHLTKVMALELAAKGI 183
++ ++ M+ +R+G+IV + S +A+YA+SKAA + TK + LELA I
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 184 RVNAICPGTIESDMHDALSDFQEGLLKR-----------TPLRRFGRQDDLDGVSLLLAS 232
R N + PG+ E+DM +L + G + PL++ + D+ L L S
Sbjct: 181 RCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240

Query: 233 DAGRYIVGAAIPVDGGQAL 251
+I + VDGG L
Sbjct: 241 GQAGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1134HTHTETR845e-22 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 83.9 bits (207), Expect = 5e-22
Identities = 46/203 (22%), Positives = 80/203 (39%), Gaps = 13/203 (6%)

Query: 33 KSQDEQYQLKRQAVIAEASRAFGHRGYQNVSLDEIAKSLNVTKPALYHYFKSKQELLYEC 92
+ ++ Q RQ ++ A R F +G + SL EIAK+ VT+ A+Y +FK K +L E
Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62

Query: 93 HNLSME-IGDMALEEAMTTGKNGLEKLEKFVSIYIRDFASE----LGASAVLHEYSGMTP 147
LS IG++ LE + L L + + + +E L + H+
Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEF--V 120

Query: 148 KDRKQIMARRRQFDLRLRDLI----QEGIDDKTIAE-CNPKLAVFWFMGAITS-IPRWYR 201
+ + +R L D I + I+ K + + A G I+ + W
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 202 LDGDLSGADIAQTFVHFLVKGIQ 224
A+ +V L++
Sbjct: 181 APQSFDLKKEARDYVAILLEMYL 203


17Bpet1155Bpet1276Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1155025-3.028890hypothetical protein
Bpet1156023-3.154363hypothetical protein
Bpet1157126-2.805315putative secreted protein
Bpet1158025-2.164948putative enoyl-CoA hydratase/isomerase
Bpet1159026-2.247386putative enoyl-CoA hydratase
Bpet1160223-2.854553IclR family transcriptional regulator
Bpet1161225-3.795458IclR family transcriptional regulator
Bpet1162221-3.682361NADH-dependent flavin oxidoreductase
Bpet1163219-4.409095hypothetical protein
Bpet1164117-4.623872short-chain dehydrogenase
Bpet1165118-4.645364putative branched-chain amino acid ABC
Bpet1166018-4.371447high-affinity branched-chain amino acid
Bpet1167119-4.659065branched-chain amino acid ABC transporter,
Bpet1168222-5.378794putative branched-chain amino acid transport
Bpet1169124-5.526601putative amino acid ABC transport system,
Bpet1170029-5.337728putative beta-hydroxyacid dehydrogenase
Bpet1171130-5.617056enoyl-CoA hydratase
Bpet1172134-5.790165LysR family transcriptional regulator
Bpet1173033-4.9772523-oxoacid CoA-transferase subunit A
Bpet1174031-4.4879473-oxoacid CoA-transferase subunit B
Bpet1175132-4.320026cation transport protein
Bpet1176132-4.213019monooxygenase
Bpet1177131-3.568038putative NADH:flavin oxidoreductase
Bpet1178230-3.384603putative monooxygenase
Bpet1179128-3.954785LysR family transcriptional regulator
Bpet1180129-3.945154putative monooxygenase
Bpet1181127-4.251522hypothetical protein
Bpet1182226-4.494847hypothetical protein
Bpet1183127-5.979179hypothetical protein
Bpet1184128-6.319947putative substrate-binding periplasmic protein
Bpet1185030-5.762285putative branched-chain amino acid transport
Bpet1186030-5.357431putative branched-chain amino acid transport
Bpet1187134-4.975257putative branched-chain amino acid ABC
Bpet1188134-5.107309putative branched-chain amino acid ABC
Bpet1189132-4.075917TetR family transcriptional regulator
Bpet1190034-3.773187LysR family transcriptional regulator
Bpet1191135-4.073536putative secreted protein
Bpet1192035-5.072285hypothetical protein
Bpet1193-136-4.713328AsnC family transcriptional regulator
Bpet1194-235-4.007121putative dehydrogenase
Bpet1195-134-4.134466hypothetical protein
Bpet1196141-6.459359LysR family transcriptional regulator
Bpet1197147-7.270393transposase IS3/IS911
Bpet1198150-7.506873transposase
Bpet1199249-7.472531putative transposase
Bpet1200249-7.587611hypothetical protein
Bpet1201249-7.587611LysR family transcriptional regulator
Bpet1202240-5.240365outer membrane efflux protein
Bpet1203236-4.840175HlyD family secretion protein
Bpet1204032-3.927182putative transposase
Bpet1205133-4.968528putative transposase
Bpet1206234-6.038916putative transposase
Bpet1207235-6.443695putative transposase
Bpet1208337-6.760346hypothetical protein
Bpet1209433-5.968770putative secreted protein
Bpet1210530-5.349184Acetyl-CoA synthetase
Bpet1211429-4.304389acyl-CoA dehydrogenase
Bpet1212427-3.638363enoyl-CoA hydratase/isomerase family protein
Bpet1213525-3.341987oxidoreductase, short-chain
Bpet1214426-4.224563acyl-CoA dehydrogenase
Bpet1215529-4.119576putative acetyl-CoA synthetase
Bpet1216430-4.286141putative dioxygenases related to 2-nitropropane
Bpet1217429-4.946796putative secreted protein
Bpet1218428-4.780004TrapT family protein
Bpet1219528-5.085733hypothetical protein
Bpet1220528-4.812086redicted acyl-CoA transferases/carnitine
Bpet1221425-5.335492hypothetical protein
Bpet1222425-5.966667enoyl-CoA hydratase
Bpet1223324-6.398753hypothetical protein
Bpet1224424-6.280387putative acyl dehydratase
Bpet1225526-6.657326putative secreted protein
Bpet1226526-5.963845putative secreted protein
Bpet1227327-6.077123acyl-CoA transferase/carnitine dehydratase
Bpet1228428-5.773745acyl dehydratase
Bpet1229630-6.624327putative thiolase
Bpet1230632-6.999717hypothetical protein
Bpet1231529-6.693691enoyl-CoA hydratase/isomerase family protein
Bpet1232425-6.679642hypothetical protein
Bpet1233524-6.294517putative secreted protein
Bpet1234424-6.057796transcriptional regulator
Bpet1235423-4.750369thioesterase superfamily protein
Bpet1236422-4.759506ABC transporter, substrate binding protein
Bpet1237523-4.561780transcriptional regulator
Bpet1238623-4.190066CAIB/BAIF family CoA transferase
Bpet1239623-3.885715putative ligase/synthetase
Bpet1240524-3.366521enoyl-CoA dehydratase
Bpet1241424-3.548168putative short chain dehydrogenase
Bpet1242525-3.802318acyl-CoA dehydrogenase
Bpet1243324-3.8687253-hydroxybutyryl-CoA dehydrogenase
Bpet1244228-4.002493hypothetical protein
Bpet1245027-5.231932hypothetical protein
Bpet1246029-5.937157NADPH quinone oxidoreductase, putative
Bpet1247-133-6.424155enoyl-CoA hydratase
Bpet1248-233-4.643280TRm3 transposase
Bpet1249-129-4.076199hypothetical protein
Bpet1250029-4.383169putative secreted protein
Bpet1251028-4.271457hypothetical protein
Bpet1252028-4.349970LysR family transcriptional regulator
Bpet1253-127-3.935867hypothetical protein
Bpet1254026-4.822969hypothetical protein
Bpet1255-229-4.715173putative secreted protein
Bpet1256-228-4.158752LysR family transcriptional regulator
Bpet1257-225-3.479768putative secreted protein
Bpet1258-221-2.967857ketoglutarate semialdehyde dehydrogenase
Bpet1259-221-3.240745sodium/alanine symporter family protein
Bpet1260-117-2.491843D-amino acid dehydrogenase, small subunit
Bpet1261016-3.188556malate dehydrogenase
Bpet1262116-3.680726hydroxyproline-2-epimerase
Bpet1263118-4.263971putative prolidase
Bpet1264121-5.356353hypothetical protein
Bpet1265217-5.801737putative transport protein
Bpet1266426-7.354801putative amino acid ABC transporter ATP binding
Bpet1267430-7.327671ABC-type amino acid transport system, permease
Bpet1268532-7.434067polar amino acid transport system permease
Bpet1269535-7.333380ABC transporter, substrate binding protein
Bpet1270628-4.959253dihydrodipicolinate synthase
Bpet1271530-3.742340transcriptional regulator
Bpet1272322-2.149640LysR family transcriptional regulator
Bpet1273115-0.362013transcriptional regulator
Bpet12740151.411697LysR family transcriptional regulator
Bpet1275-1122.013954phage-related integrase
Bpet12762143.784273*hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1164DHBDHDRGNASE1154e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 115 bits (288), Expect = 4e-33
Identities = 75/258 (29%), Positives = 117/258 (45%), Gaps = 13/258 (5%)

Query: 4 LEGKVAFITGGGAGIGCASALLFAQEGAQVVIAERDTAAGEQTAAIVEKSTGRPARFIHT 63
+EGK+AFITG GIG A A A +GA + + + E+ + K+ R A
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVS-SLKAEARHAEAFPA 64

Query: 64 DVTEPESLEAAVKRTVAEFGRFDVLYNNAGGSTVRDSRVTDAPVDEFWSKMKLDLFGTWL 123
DV + +++ R E G D+L N AG +R + +E+ + ++ G
Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAG--VLRPGLIHSLSDEEWEATFSVNSTGV-F 121

Query: 124 GCRYGIQAMMDAGNGGSVINSTSIFALIGTHGKDAYTAAKGAVSALTRSMAVEYAQYRIR 183
+ M GS++ S A + AY ++K A T+ + +E A+Y IR
Sbjct: 122 NASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181

Query: 184 VNAVAPGATATERVLKLLKDDGVTSKSLDGQ---------LFGLVQPEDIAHAALYLASD 234
N V+PG+T T+ L D+ + + G L L +P DIA A L+L S
Sbjct: 182 CNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 235 ESRSTTGHILAVDGGLTI 252
++ T H L VDGG T+
Sbjct: 242 QAGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1189HTHTETR552e-11 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.4 bits (133), Expect = 2e-11
Identities = 28/159 (17%), Positives = 59/159 (37%), Gaps = 10/159 (6%)

Query: 25 RRLQPEEREQQIVEKAIEHFTRNGFEG-STRELAKQIGVTQPLLYRYFSSKEALIERVYT 83
+ + +E Q I++ A+ F++ G S E+AK GVT+ +Y +F K L ++
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 84 EVFQWRREWEAQIKDRSVP-----LRDRLHAFYMDYSTVILREEWIRIFIFAGLTRDGIN 138
E E + + + LR+ L ++ + R + IF G
Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIH-VLESTVTEERRRLLMEIIFHKCEFVG-E 122

Query: 139 NRYLTRLRTQVFLPVLAELRAEF--GVDEPRDRAEVDAE 175
+ + + + L + ++ A++
Sbjct: 123 MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTR 161


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1203RTXTOXIND565e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 56.0 bits (135), Expect = 5e-11
Identities = 23/150 (15%), Positives = 54/150 (36%), Gaps = 9/150 (6%)

Query: 82 RVRLANDLAEAKASVATAKAQLAAAEREDKRYRGLADVVAPQELDVRRTAAETARAQYEQ 141
V N+L K+ + ++++ +A+ E + L +L
Sbjct: 261 YVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKL-------RQTTDNIGL 313

Query: 142 AIASLDRARINLERAEVRSPVNGIITNFSLL-PGAYAIAGQPVMALV-DQDSFYVAGYFE 199
L + + + +R+PV+ + + G + +M +V + D+ V +
Sbjct: 314 LTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQ 373

Query: 200 ETKLSRMGVGMPATIHLMGESRSLKGHVEG 229
+ + VG A I + + G++ G
Sbjct: 374 NKDIGFINVGQNAIIKVEAFPYTRYGYLVG 403



Score = 54.1 bits (130), Expect = 2e-10
Identities = 24/163 (14%), Positives = 56/163 (34%), Gaps = 12/163 (7%)

Query: 6 QSSRVLKIALTLALAVLGAFTLWHLYAYYTYSPQTRDGKVRAD--VVALAADVSGRVDEV 63
SR ++ + L + + T +GK+ + + V E+
Sbjct: 52 PVSRRPRLVAYFIMGFLVIAFILSVLGQVE-IVATANGKLTHSGRSKEIKPIENSIVKEI 110

Query: 64 RVRDNQVVKHGDVLFVIDRVRLANDLAEAKASVATAKAQLA--AAEREDKRYRGLADVVA 121
V++ + V+ GDVL + + D + ++S+ A+ + L ++
Sbjct: 111 IVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKL 170

Query: 122 PQELDVRRTAAE-------TARAQYEQAIASLDRARINLERAE 157
P E + + E + Q+ + +NL++
Sbjct: 171 PDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKR 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1213DHBDHDRGNASE1017e-28 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 101 bits (253), Expect = 7e-28
Identities = 72/256 (28%), Positives = 113/256 (44%), Gaps = 10/256 (3%)

Query: 7 LQGRVAMITGGAGGIGLDIAKTYGRLGARVVLASRNQDRLDHAAAQLSEEGIDVLAVRAD 66
++G++A ITG A GIG +A+T GA + N ++L+ + L E A AD
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 67 VRNYDEVKAAVESAVTHFGALDILVNNAAGNFYCPTAELSPNGWRTVIDIDLNGTFYGCH 126
VR+ + G +DILVN A LS W ++ G F
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 127 AAYKHLKQSPFGGCIISIVTMLGLSGWPGAAHAGAAKAGILSLSRTLAVEWGADNIRVNT 186
+ K++ G I+++ + A ++KA + ++ L +E NIR N
Sbjct: 126 SVSKYMMDRR-SGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 187 ISPGPIGDTEGVRRLYQETGREE------LERKKTA--LGRFGRKTDIANAATYLASDMA 238
+SPG +T+ L+ + E LE KT L + + +DIA+A +L S A
Sbjct: 185 VSPGST-ETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 239 AYITGENMIVDGGRWL 254
+IT N+ VDGG L
Sbjct: 244 GHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1219MECHCHANNEL280.040 Bacterial mechano-sensitive ion channel signature.
		>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature.

Length = 136

Score = 27.9 bits (62), Expect = 0.040
Identities = 13/29 (44%), Positives = 17/29 (58%)

Query: 212 MSWLKTLSLWDMTKNVVDLPMGAFLGGAF 240
MS +K + M NVVDL +G +G AF
Sbjct: 1 MSIIKEFREFAMRGNVVDLAVGVIIGAAF 29


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1234HTHFIS354e-119 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 354 bits (911), Expect = e-119
Identities = 124/366 (33%), Positives = 191/366 (52%), Gaps = 29/366 (7%)

Query: 217 SLSQEVTSLRSELAFYRREYSGAHPYPNELQQIVGDSDAIRQLKSDIVKVAPLNVPVLII 276
L++ + + LA +R S + +VG S A++++ + ++ ++ ++I
Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMIT 166

Query: 277 GESGTGKELAARAIHELSPRSKKRMVFVNAAALPTSLVESELFGYEGGAFTGAGKAGRKG 336
GESGTGKEL ARA+H+ R V +N AA+P L+ESELFG+E GAFTGA + G
Sbjct: 167 GESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGA-QTRSTG 225

Query: 337 KFEMADSGSLFFDEIGDMPIEIQVKLLRILQDGVYERLGGNQVGHSDFRLICASNRNFQS 396
+FE A+ G+LF DEIGDMP++ Q +LLR+LQ G Y +GG SD R++ A+N++ +
Sbjct: 226 RFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQ 285

Query: 397 MIRDGQFRLDLYYRISGVTIRMPSLRERLDDIPVLVQSILVSFADRHRASVKLVSPRVYD 456
I G FR DLYYR++ V +R+P LR+R +DIP LV+ + + VK +
Sbjct: 286 SINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALE 344

Query: 457 FLREQPWPGNVRQLLHEVEKAAIFCDGPEISIENFRLTT--KIPAPGTAEIRPWEEQQTI 514
++ PWPGNVR+L + V + I+ E +IP + +I
Sbjct: 345 LMKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSI 404

Query: 515 PAAI-------------------------ESLERNMVKEALLRHRGNKKKAAEELGISRA 549
A+ +E ++ AL RGN+ KAA+ LG++R
Sbjct: 405 SQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRN 464

Query: 550 YLYKKL 555
L KK+
Sbjct: 465 TLRKKI 470


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1237HTHFIS366e-123 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 366 bits (942), Expect = e-123
Identities = 129/369 (34%), Positives = 210/369 (56%), Gaps = 19/369 (5%)

Query: 210 MGDEIKRLQQEISFYQRSLPRLSGNMQGMEFIVGESDAIRKLKERIKKIARLDVSVLLVG 269
+ + I + + ++ +R +L + Q +VG S A++++ + ++ + D+++++ G
Sbjct: 108 LTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITG 167

Query: 270 ESGVGKDLVAHAIHQLSPRAARDMVLVNAAAIPGNLVEAELFGYEGGAFTGAEKRGRTGK 329
ESG GK+LVA A+H R V +N AAIP +L+E+ELFG+E GAFTGA + TG+
Sbjct: 168 ESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGA-QTRSTGR 226

Query: 330 FEQADQTTLFLDEIGDMPLDIQVKVLRTLQDGTFQRVGSAAQRHSDFRLISATNRDFQRM 389
FEQA+ TLFLDEIGDMP+D Q ++LR LQ G + VG SD R+++ATN+D ++
Sbjct: 227 FEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQS 286

Query: 390 LATGDFRLDLFYRISAVTIRVPPLRERLEDVPLLAHTFLERFMQRHNVQGKHFGPGVMAY 449
+ G FR DL+YR++ V +R+PPLR+R ED+P L F+++ + + K F +
Sbjct: 287 INQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALEL 345

Query: 450 LQSLPWPGNVRQLQHTVERAAIFSEQDEISCEDC------EVPLEVDSGPGIQGITGTLR 503
+++ PWPGNVR+L++ V R QD I+ E E+P + + ++
Sbjct: 346 MKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSIS 405

Query: 504 E---------MNMPDDSLSPGKLPSVHQAKSRIELGMIREALQRFEGNKKKAAEYLGISR 554
+ D+L P L + + +E +I AL GN+ KAA+ LG++R
Sbjct: 406 QAVEENMRQYFASFGDALPPSGL--YDRVLAEMEYPLILAALTATRGNQIKAADLLGLNR 463

Query: 555 SHLYKILSE 563
+ L K + E
Sbjct: 464 NTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1241DHBDHDRGNASE792e-19 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 78.6 bits (193), Expect = 2e-19
Identities = 65/256 (25%), Positives = 112/256 (43%), Gaps = 14/256 (5%)

Query: 4 ELIYVTGASRGIGAMIACQLARRGFEVGCLSRSGHRPQVENVPGEIAARW-HTVACDVTD 62
++ ++TGA++GIG +A LA +G + + + + + + AR DV D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 63 GEALKDAMSKLRDDLGISVRGLVNNAGLHTEAPSVDLPMDEFRRLMDINAVSLLRACQIA 122
A+ + +++ ++G + LVN AG+ L +E+ +N+ + A +
Sbjct: 69 SAAIDEITARIEREMG-PIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 123 YPMLKESGGGLIVNIGSFYDKLGVKRN--IAYCASKAAVGAITRCLAVEWARDGIQVIDV 180
+ + G IV +GS + GV R AY +SKAA T+CL +E A I+ V
Sbjct: 128 SKYMMDRRSGSIVTVGS--NPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 181 APGYIETDLNR--------EAMQAGPLREYLEKRIPRKKPGEAGDVAVLVSSLFQPGMEF 232
+PG ETD+ E + IP KK + D+A V L
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 233 LTGETIYIDGAQSVSV 248
+T + +DG ++ V
Sbjct: 246 ITMHNLCVDGGATLGV 261


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1254YERSSTKINASE290.049 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 29.3 bits (65), Expect = 0.049
Identities = 16/36 (44%), Positives = 24/36 (66%), Gaps = 5/36 (13%)

Query: 180 VSDIYSEQLYIGGLLSTVWVSDYDDMMVALDQDDRE 215
+S ++QL +GG+LS D D M+VALD+ +RE
Sbjct: 456 LSSAATKQLDMGGVLS-----DLDTMLVALDKAERE 486


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1263UREASE290.036 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 29.3 bits (66), Expect = 0.036
Identities = 12/26 (46%), Positives = 15/26 (57%)

Query: 37 PGADVIDVGGKTVMPGLIDCHVHVIA 62
PG +VI GK V G +D H+H I
Sbjct: 116 PGTEVIAGEGKIVTAGGMDSHIHFIC 141


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1265TCRTETA553e-10 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 54.8 bits (132), Expect = 3e-10
Identities = 44/155 (28%), Positives = 71/155 (45%), Gaps = 4/155 (2%)

Query: 19 KLLLMGGLGFAFEALDAGIIAFILPVLRTQWSLSSLEV---GFLASSTYIGFLIGALLAG 75
+ L++ A +A+ G+I +LP L S+ G L + + A + G
Sbjct: 5 RPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLG 64

Query: 76 LLGDRFGRRGVMMWALAIFCVMSIANAMTHDWHLFFLFRMLAGIGMGAEGAIIAPFLAEF 135
L DRFGRR V++ +LA V A + ++ R++AGI GA GA+ ++A+
Sbjct: 65 ALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADI 123

Query: 136 VASRYRGRFTGSLAGFFSFGFVIAALLGYFIVPLS 170
R R G ++ F FG V +LG + S
Sbjct: 124 TDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFS 158



Score = 38.3 bits (89), Expect = 6e-05
Identities = 33/142 (23%), Positives = 55/142 (38%), Gaps = 5/142 (3%)

Query: 51 LSSLEVGF-LASSTYIGFLIGALLAGLLGDRFGRRGVMMWALAI-FCVMSIANAMTHDWH 108
+ +G LA+ + L A++ G + R G R +M + + T W
Sbjct: 243 WDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWM 302

Query: 109 LFFLFRMLAGIGMGAEGAIIAPFLAEFVASRYRGRFTGSLAGFFSFGFVIAALLGYFIVP 168
F + +LA G+G + L+ V +G+ GSLA S ++ LL I
Sbjct: 303 AFPIMVLLASGGIGMPA--LQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYA 360

Query: 169 LSDNGWR-WVLVISAVPVVVLL 189
S W W + A ++ L
Sbjct: 361 ASITTWNGWAWIAGAALYLLCL 382


18Bpet1286Bpet1382Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1286027-3.365845short chain dehydrogenase
Bpet1287232-4.649836hypothetical protein
Bpet1288433-4.735657LysR family transcriptional regulator
Bpet1289231-4.657317putative transcriptional regulator
Bpet1290231-4.278686hypothetical protein
ig_0609229-4.171062hypothetical protein
Bpet1291128-3.841546hypothetical protein
Bpet1292126-3.907321hypothetical protein
Bpet1293123-3.599991hypothetical protein
Bpet1294119-3.096328hypothetical protein
Bpet1295119-3.384378transcriptional regulator
Bpet1296219-3.447650adenine DNA methyltransferase protein
Bpet1297322-3.737052single-stranded DNA-binding protein
Bpet1298126-5.893024hypothetical protein
Bpet1299331-7.761800transposase
Bpet1300335-8.709274transposase
Bpet1301338-8.049141hypothetical protein
Bpet1302342-8.527029putative C-5 cytosine-specific DNA methylase
Bpet1303343-8.851341integrase/recombinase
Bpet1304441-8.107249integrase/recombinase
Bpet1305239-6.777927putative integrase/recombinase
Bpet1306133-4.857223DNA-cytosine methyltransferase
Bpet1307333-5.955847acetyltransferase
Bpet1308431-6.201184hypothetical protein
Bpet1309431-6.185512hypothetical protein
Bpet1310529-5.150520hypothetical protein
Bpet1311728-5.440302hypothetical protein
Bpet1312631-6.628755hypothetical protein
Bpet1313530-6.302350hypothetical protein
Bpet1314428-5.459429hypothetical protein
Bpet1315528-5.501803hypothetical protein
Bpet1316328-5.803444hypothetical protein
Bpet1317330-5.462070hypothetical protein
Bpet1318426-4.801734hypothetical protein
Bpet1319529-5.621770hypothetical protein
Bpet1320434-7.485886hypothetical protein
Bpet1321236-7.971919hypothetical protein
Bpet1322339-8.371181hypothetical protein
Bpet1323439-8.900635hypothetical protein
Bpet1324450-10.585779putative DNA-binding protein
Bpet1325345-9.3050875-aminolevulinate synthase
Bpet1326341-8.176081acetyltransferase
Bpet1327440-8.029815putative oxidoreductase
Bpet1328338-7.122572asparagine synthetase, glutamine-hydrolyzing
Bpet1329238-6.626972MFS transporter
Bpet1330135-6.044777putative monooxygenase
Bpet1331238-7.7736662,4-dichlorophenol 6-monooxygenase
Bpet1332140-8.218728TetR family transcriptional regulator
Bpet1333134-7.268649major facilitator superfamily permease
Bpet1334336-9.266310hypothetical protein
Bpet1335336-8.478648hypothetical protein
Bpet1336334-8.585665hypothetical protein
Bpet1337334-7.314265transposase
Bpet1338437-7.316502transposase
Bpet1339240-7.177424hypothetical protein
Bpet1340236-5.670158threonine efflux protein
Bpet1341135-5.066789hypothetical protein
Bpet1342033-3.575895alanyl-tRNA synthetase domain-containing
Bpet1343132-2.269162LysR family transcriptional regulator
Bpet1344-125-1.759684putative lipoprotein
Bpet1345-123-2.236613hypothetical protein
Bpet1346-123-2.719906hypothetical protein
Bpet1347021-2.545218hypothetical protein
Bpet1348121-2.826262putative secreted protein
Bpet1349021-2.940114hypothetical protein
Bpet1350223-2.724883hypothetical protein
Bpet1351124-1.981748carbon storage regulator
Bpet1352122-0.641883hypothetical protein
Bpet13533190.229140putative DNA-binding protein
Bpet13543190.717270putative secreted protein
Bpet1355215-0.032022hypothetical protein
Bpet1356216-0.303808hypothetical protein
Bpet1357218-1.562365hypothetical protein
Bpet1358119-2.595578hypothetical protein
Bpet1359021-2.782139hypothetical protein
Bpet1360120-3.309243hypothetical protein
Bpet1361122-3.869228putative lipoprotein
Bpet1362223-4.088507hypothetical protein
Bpet1363327-4.600906signalling protein
Bpet1364330-4.521946hypothetical protein
Bpet1365329-4.529711hypothetical protein
Bpet1366330-4.740188hypothetical protein
Bpet1367229-5.084387TetR family transcriptional regulator
Bpet1368327-5.048804cAMP phosphodiesterase
Bpet1369227-4.686873hypothetical protein
Bpet1370323-3.689612LysR family transcriptional regulator
Bpet1371425-3.625597hypothetical protein
Bpet1372324-3.315593putative amino acid efflux transmembrane
Bpet1373424-3.334612phosphoserine phosphatase
Bpet1374426-3.257877hypothetical protein
Bpet1375525-4.005189hypothetical protein
Bpet1376427-5.287631hypothetical protein
Bpet1377331-6.123309hypothetical protein
Bpet1378331-5.578545hypothetical protein
Bpet1379232-5.214581hypothetical protein
Bpet1380229-4.884333hypothetical protein
Bpet1381128-4.049755hypothetical protein
Bpet1382125-3.586327putative helicase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1286DHBDHDRGNASE1164e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 116 bits (291), Expect = 4e-33
Identities = 75/264 (28%), Positives = 124/264 (46%), Gaps = 21/264 (7%)

Query: 31 LAGKVALVSGGGSSGPGWSIGKASCATLARHGAVVCVLDASLEAAQDALAAVQALGGQGL 90
+ GK+A ++G IG+A TLA GA + +D + E + +++++A
Sbjct: 6 IEGKIAFITGAAQG-----IGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 91 ALQADVADAVAMERAVQAVMDRYGRLDILQANAGIGKVGGPEDTALADWERIQKVNVDSL 150
A ADV D+ A++ + G +DIL AG+ + G + +WE VN +
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 151 LIASRLVLPIMSQQGGGAIVTVSSVAGLRYLGYPHL---AYNVTKAAVIHFARMIAQQYA 207
ASR V M + G+IVTV S G P AY +KAA + F + + + A
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPA----GVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 208 GQGIRANTVVPGLIDTPRVRNTVARMFSADDFEQARAARDRQ-----VPMGRMGTPWEVA 262
IR N V PG +T + +++ ++ + + +P+ ++ P ++A
Sbjct: 177 EYNIRCNIVSPGSTETDMQWS----LWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIA 232

Query: 263 NAVAFLASDEASYITGTELVVDGG 286
+AV FL S +A +IT L VDGG
Sbjct: 233 DAVLFLVSGQAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1291IGASERPTASE372e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 37.4 bits (86), Expect = 2e-04
Identities = 18/111 (16%), Positives = 29/111 (26%), Gaps = 14/111 (12%)

Query: 291 SEKRWQVLTSEPAAAPPATPAVATL------PQTAPAQLSPPTPSTPPQRETPVAASESS 344
S K+ Q T +P A P A P T E PV S +
Sbjct: 1130 SPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTV 1189

Query: 345 LQTKPSPMQPPASDDQPDDESQRSALLQEHI-------ISPAPTTDRLQSI 388
T S ++ P + + ++ + P +
Sbjct: 1190 -NTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATT 1239


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1299HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1314TONBPROTEIN270.038 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 27.3 bits (60), Expect = 0.038
Identities = 15/52 (28%), Positives = 18/52 (34%), Gaps = 2/52 (3%)

Query: 119 IKVDGVLKYKAEPKPAGSEQDAAEQSAAPAPAPQEVPVPEASEPVNEPADEA 170
I V V EP A Q E P P P+ +P P PV +
Sbjct: 45 ISVTMVTPADLEPPQA--VQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKP 94


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1318PHPHTRNFRASE280.036 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 27.8 bits (62), Expect = 0.036
Identities = 11/49 (22%), Positives = 19/49 (38%)

Query: 155 LDLLMEQSMLARLPMAYGLLEGHRLALDVPVLTKALGDLIRNGTLSATA 203
L + +Q+ + + H L LD P L + I N ++A
Sbjct: 55 LRAIKDQTEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEY 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1326SACTRNSFRASE334e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 32.6 bits (74), Expect = 4e-04
Identities = 15/63 (23%), Positives = 32/63 (50%), Gaps = 5/63 (7%)

Query: 94 SGWCGW--VQSMVVSPSWRRMGIAESLMHELLQWFSLLGVTKVVLESTQV---AEAMYQK 148
S W G+ ++ + V+ +R+ G+ +L+H+ ++W ++LE+ + A Y K
Sbjct: 84 SNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAK 143

Query: 149 LGF 151
F
Sbjct: 144 HHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1329TCRTETB509e-09 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 49.9 bits (119), Expect = 9e-09
Identities = 79/375 (21%), Positives = 145/375 (38%), Gaps = 52/375 (13%)

Query: 43 LPMIETAFSVPVAIAAQLVTAFTLAYGLGSPIFVALLPAHQQRAGLLFALGLFVLANAAS 102
LP I F+ P A + TAF L + +G+ ++ L + LLF + + +
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 103 ALS-TDFTVLMVFRAIAGIGAGVYLAMGIAASAALSPPDQRGKSIAVIMGGMASGTVLGV 161
+ + F++L++ R I G GA + A+ + A P + RGK+ +I +A G +G
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 162 PLSLLLAEQLGWESALWLVTLLGAIAFVGLIARLPSLPTVQAIPLKAKIALLTDSHVVVI 221
+ ++A + W S L L+ ++ I L+ L ++ I L++ V +
Sbjct: 157 AIGGMIAHYIHW-SYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFM 215

Query: 222 LLVS----LLAAISSLGMYTFLAPLIAAAEPNSSP------------------------- 252
L + +S L F+ + +P P
Sbjct: 216 LFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGF 275

Query: 253 -SVTPY-----------------LWVWGVGGVLGSFLIGPLVDRVKGPTLTLWI-MAILA 293
S+ PY ++ + ++ ++ G LVDR +GP L I + L+
Sbjct: 276 VSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDR-RGPLYVLNIGVTFLS 334

Query: 294 VALLLLPASLSTGPWLVMLPIAIWGAVGWALQVPQNNELIKAREQQGDGNLAVALNESAL 353
V+ L L T W + + I + + + + +QQ G LN ++
Sbjct: 335 VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS- 393

Query: 354 YLGSALGAAMGGVLL 368
+L G A+ G LL
Sbjct: 394 FLSEGTGIAIVGGLL 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1332HTHTETR601e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 60.4 bits (146), Expect = 1e-13
Identities = 30/173 (17%), Positives = 69/173 (39%), Gaps = 5/173 (2%)

Query: 13 SRERGRPREFDIHTALDRAILYFREHGYNGVSIADLSQALKLSAGSIYKAFHSKHGLFTA 72
+++ + I LD A+ F + G + S+ ++++A ++ G+IY F K LF+
Sbjct: 5 TKQEAQETRQHI---LDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 73 ALDRYMALRGQQIAEITASAESG-REKLRRLLVFYAESSHAAEGRYGCLVVVGAVELSST 131
+ + G+ E A LR +L+ ES+ E R + ++
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121

Query: 132 DEAIAAKV-ASALSANERRLKAIIEQGQQDGSISRTAEPGTTAKLMLALLQGM 183
+ A+ + + + R++ ++ + + A +M + G+
Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1333TCRTETB393e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 38.7 bits (90), Expect = 3e-05
Identities = 64/372 (17%), Positives = 119/372 (31%), Gaps = 45/372 (12%)

Query: 47 ISAQLNLSEQASGLIVTLTQLGYGLGLLLVVPLGDLFENRRLAISILAVGAIGLLISGFA 106
I+ N ++ + T L + +G + L D +RL + + + G +I
Sbjct: 40 IANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVG 99

Query: 107 GSVEPFL-AASFLVGLGSVTVQILVPYA-AHLAPDATRGRVVGNVMSGLMLGIMLARPVA 164
S L A F+ G G+ LV A P RG+ G + S + +G + +
Sbjct: 100 HSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIG 159

Query: 165 SMITYFTSWRVVFFLSFIGMVLLAGVLRFALPTRPVVARLRY-HQMLASMA--------- 214
MI ++ W + + I ++ + +++ + +L S+
Sbjct: 160 GMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT 219

Query: 215 -------------------HLVRTT-----PALRRRALYHASMFGAFSLFWTTTPLLLAG 250
H+ + T P L + + + +F T +
Sbjct: 220 SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMV 279

Query: 251 PQ-----FGLS--QKGIALFALAGVAGAIAAPIAGRIADRGWIRSATAAAMLLGIGAFAI 303
P LS + G + ++ I I G + DR + +F
Sbjct: 280 PYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLT 339

Query: 304 TYIGDIGTSLSLAMFVIAGVVLDFAVSANLVLGQRVIFSLAPEIRGRLNGLYMTTFFCGG 363
TS + + ++ VL V+ V SL + G L T F
Sbjct: 340 ASFLLETTSWFMTIIIVF--VLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSE 397

Query: 364 AIGSALGGWLFA 375
G A+ G L +
Sbjct: 398 GTGIAIVGGLLS 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1337HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1345RTXTOXIND320.001 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.5 bits (74), Expect = 0.001
Identities = 17/93 (18%), Positives = 30/93 (32%), Gaps = 8/93 (8%)

Query: 37 ALSNLATKAGGSAQSTQVAALETRLAELGQQLEAQRQQPDTLTTAQFETERQAIEQRVSR 96
S+L K + V E + E +L + Q Q E+E + ++
Sbjct: 239 DFSSLLHK--QAIAKHAVLEQENKYVEAVNELRVYKSQ-----LEQIESEILSAKEEYQL 291

Query: 97 IEQALGERLTAESLSPLHSRIEQLESRLTKAAQ 129
+ Q + + L I L L K +
Sbjct: 292 VTQLF-KNEILDKLRQTTDNIGLLTLELAKNEE 323


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1346BCTERIALGSPD300.013 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 29.5 bits (66), Expect = 0.013
Identities = 15/58 (25%), Positives = 21/58 (36%), Gaps = 4/58 (6%)

Query: 94 DERRRYAELQVQVEARRVEKTLAYQRAYDAAWQRLHPGMQRVNLPGANIASGASATSS 151
D RR QV VEA E A W + GM + G I++ + +
Sbjct: 341 DIRRP----QVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQ 394


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1351adhesinmafb250.024 Neisseria meningitidis: adhesin MafB signature.
		>adhesinmafb#Neisseria meningitidis: adhesin MafB signature.

Length = 467

Score = 25.0 bits (54), Expect = 0.024
Identities = 6/24 (25%), Positives = 14/24 (58%)

Query: 40 VDVHRQEIYERIHPGSASHFSGKH 63
+D+H +++ R GS+ +G+
Sbjct: 367 LDIHYEDLIRRKTDGSSKFINGRE 390


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1360FbpA_PF05833330.002 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 33.3 bits (76), Expect = 0.002
Identities = 6/53 (11%), Positives = 20/53 (37%), Gaps = 4/53 (7%)

Query: 84 TQKAENERLRQREGAIDRRIQSALETERNQLKNDREQVA----SERQQTQGLL 132
K +++RL+ + + + + + + + K + + + G L
Sbjct: 289 YAKDKSDRLKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCEDKDIFKLYGEL 341


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1367HTHTETR701e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 70.0 bits (171), Expect = 1e-16
Identities = 37/174 (21%), Positives = 75/174 (43%), Gaps = 3/174 (1%)

Query: 43 EERCKRILEAAERVFARVGYGAATMEEMAREAGMSKRTLYAFYADKRELFTAVIGDVEGF 102
+E + IL+ A R+F++ G + ++ E+A+ AG+++ +Y + DK +LF+ + E
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 103 SGKRPHVPAAAGRDALIVELHDRLLEMARFVLSERQIRITRLII-SEAENHPELAVDFWS 161
G+ A + L + L+ + ++E + R+ II + E E+AV +
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 162 R--IVVRVQAYLVDGLKELQRADETLREYDANRLASTIFGAILSDLHLRTLFGQ 213
+ + + + LK A + R A + G I + Q
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQ 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1368FLGMRINGFLIF330.004 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 33.4 bits (76), Expect = 0.004
Identities = 23/94 (24%), Positives = 32/94 (34%), Gaps = 6/94 (6%)

Query: 486 GNRLKSHLDDGQFLARVEGDMFVLVVPDCDVHRAALVAAHLQRVAGAPIDISGFSLDPTV 545
G + + L R + VP VH L A G + GF L
Sbjct: 62 GGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAV---GFELLDQE 118

Query: 546 SIGISQYPESSRDREDL---LRNAKSAMGQVKAS 576
GISQ+ E + L L +G VK++
Sbjct: 119 KFGISQFSEQVNYQRALEGELARTIETLGPVKSA 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1382PF03544300.023 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 29.9 bits (67), Expect = 0.023
Identities = 20/102 (19%), Positives = 29/102 (28%), Gaps = 17/102 (16%)

Query: 386 TSAAASPATSTAAEPPSTTSVTPTPVISPRSPVAQAVSNDGVDALLDLLGTPDIEPPPIE 445
TS A+P S T V P + P++ P +EP P
Sbjct: 35 TSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPP--------------EPVVEPEPEP 80

Query: 446 APLNEPT---VPDQPLEPIDPEPTAPQSEIEAKDARTSPSGE 484
P+ EP P+P + + R E
Sbjct: 81 EPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVE 122


19Bpet1395Bpet1540Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1395213-2.643858hypothetical protein
Bpet1396111-2.0814001,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
Bpet1397211-2.970603ferredoxin--NAD(+) reductase
Bpet1398214-3.785367benzoate 1,2-dioxygenase
Bpet1399215-3.543962benzoate 1,2-dioxygenase subunit alpha
Bpet1400117-3.674740catechol 1,2-dioxygenase
Bpet1401019-4.179380chloromuconate cycloisomerase
Bpet1402326-6.054939transcriptional regulator catR
Bpet1403332-4.812686putative transposase
Bpet1404527-2.246298IS4 family transposase
Bpet1405525-1.681451putative transposase A
Bpet1406726-1.389291putative transposase
Bpet1407623-0.085733hypothetical protein
Bpet1408622-0.149836hypothetical protein
Bpet1409623-0.561405transposase
Bpet1410523-1.269991integrase catalytic subunit
Bpet1411725-2.190604transposase IS911 HTH and LZ region
Bpet1412520-1.979432hypothetical protein
Bpet1413524-3.551475hypothetical protein
Bpet1414624-3.856061hypothetical protein
Bpet1415426-4.918743nitrile hydratase subunit beta (Nitrilase)
Bpet1416325-4.578583nitrile hydratase alpha subunit
Bpet1417227-4.081633glutamyl-tRNA(Gln) amidotransferase subunit A
Bpet1418233-5.108358hypothetical protein
Bpet1419133-5.413316IS4 family transposase
Bpet1420232-5.323350hypothetical protein
Bpet1421230-4.531226hypothetical protein
Bpet1422337-7.524437hypothetical protein
Bpet1423338-8.564868hypothetical protein
Bpet1424438-8.236690IS5 family transposase orfA
Bpet1425437-7.7440923-oxoacid CoA-transferase subunit A, fragment
Bpet1426438-8.0192053-oxoacid CoA-transferase subunit B, fragment
Bpet1427438-7.556997transcriptional regulatory protein
Bpet1428438-6.986629salicylate hydroxylase
Bpet1429438-6.7096652-hydroxyhepta-2,4-diene-1,7-dioate isomerase
Bpet1430339-6.895354putative gentisate 1,2-dioxygenase
Bpet1431037-6.066999putative 4-hydroxybenzoate transporter
Bpet1432233-5.486374putative resolvase
Bpet1433132-5.573549putative transcriptional regulator
Bpet1434130-5.422493transcriptional regulator
Bpet1435127-4.249320TetR family transcriptional regulator
Bpet1436125-3.661775putative transmembrane efflux protein of the MFS
Bpet1437327-2.946688phage-related integrase
Bpet1438425-1.795075hypothetical protein
Bpet1439528-1.459657transcriptional regulator
Bpet1440427-1.363776hypothetical protein
Bpet1441327-1.883667hypothetical protein
Bpet1442426-2.081479hypothetical protein
Bpet1443321-1.974548hypothetical protein
Bpet1444220-1.347502hypothetical protein
Bpet1445119-3.383314hypothetical protein
Bpet1446224-5.151583hypothetical protein
Bpet1447324-5.214317single-stranded DNA-binding protein
Bpet1448225-5.416940DNA topoisomerase III
Bpet1449335-8.772913putative DNA-cytosine methyltransferase
Bpet1450234-8.120457integrase/recombinase
Bpet1451229-6.436451integrase/recombinase
Bpet1452127-4.503326transposase
Bpet1453128-4.610620transposase
Bpet1454228-4.733484putative integrase/recombinase
Bpet1455124-2.700462DNA-cytosine methyltransferase
Bpet1456425-4.247469hypothetical protein
Bpet1457426-4.366123hypothetical protein
Bpet1458423-4.609123hypothetical protein
Bpet1459322-4.702123hypothetical protein
Bpet1460422-4.213410hypothetical protein
Bpet1461318-3.069630hypothetical protein
Bpet1462218-2.493856hypothetical protein
Bpet1463318-1.527090transposase
Bpet1464317-0.614000transposase
Bpet1465419-0.961841hypothetical protein
Bpet1466320-0.935207hypothetical protein
Bpet1467626-1.749327transposon
Bpet1468625-1.677741hypothetical protein
Bpet1469623-1.749170hypothetical protein
Bpet1470527-5.402890hypothetical protein
Bpet1471524-4.235341hypothetical protein
Bpet1472622-4.132841hypothetical protein
Bpet1473521-3.504908hypothetical protein
Bpet1474421-3.157082hypothetical protein
Bpet1475420-2.910972mobile mitochondrial group II intron of COX1
Bpet14764190.645619hypothetical protein
Bpet14773202.642804hypothetical protein
Bpet14782211.740500putative secreted protein
Bpet14792211.124026hypothetical protein
Bpet14803210.241360hypothetical protein
Bpet14813220.210830hypothetical protein
Bpet1482423-0.558463hypothetical protein
Bpet1483322-1.581875putative plasmid-transfer-protein
Bpet1484524-0.963561hypothetical protein
Bpet14855260.001588hypothetical protein
Bpet14864212.139969hypothetical protein
Bpet14873191.053320hypothetical protein
Bpet14883171.293397hypothetical protein
Bpet14893161.766530hypothetical protein
Bpet14902171.238710hypothetical protein
Bpet14912160.268560putative secreted protein
Bpet1492318-0.747479hypothetical protein
Bpet1493319-0.546166putative lipoprotein
Bpetpseudo_04320-1.508490hypothetical protein
Bpet1494320-3.215459transposase
Bpet1495422-3.256191transposase
Bpetpseudo_05324-3.371846hypothetical protein
Bpet1496328-3.785322putative protein-disulfide isomerase
Bpet1497428-3.736709putative DNA repair protein RadC
Bpet1498425-2.619076putative integrase/recombinase
Bpet1499425-1.514721putative integrase/recombinase
Bpet1500422-1.660522putative integrase/recombinase
Bpet1501423-1.115690hypothetical protein
Bpet1502324-1.883574hypothetical protein
Bpet1503323-2.250377hypothetical protein
Bpet1504122-2.410131hypothetical protein
Bpet1505123-2.911482hypothetical protein
Bpet1506-133-4.375294putative transposon
Bpet1507138-6.408175hypothetical protein
Bpet1508341-7.668097suppressor protein
Bpet1509445-8.741312putative helicase
Bpet1510347-9.467905putative transposon
Bpet1511233-7.471688putative transcriptional regulator
Bpet1512229-6.256004glucose 1-dehydrogenase
Bpet1513024-3.600235biphenyl dioxygenase small subunit
Bpetpseudo_06122-2.514027hypothetical protein
Bpet1514221-1.758008putative transposase
Bpet1515120-1.846355dihydrolipoamide dehydrogenase
Bpet1516229-2.558274putative acyl-CoA thioester hydrolase
Bpet1517229-2.031785glutathione reductase
Bpet1518123-0.779478glutathione S-transferase
Bpet15192171.152694putative glutathione transferase
Bpet15203182.668516hypothetical protein
Bpet15214193.261973hypothetical protein
Bpet15226164.259368YciI-like protein
Bpet15235163.878582transposition-related fusion-protein
Bpet15245173.047800glycosyltransferase
Bpet15255191.639419glycosyltransferase
Bpet15265200.000692acetyltransferase
Bpet1527622-0.582601hypothetical protein
Bpet1528131-4.900632putative transposase
Bpet1529228-4.872058putative transposase
Bpet1530026-6.037530ISSod11, transposase
Bpet1531130-6.244697hypothetical protein
Bpet1532130-5.945879hypothetical protein
Bpet1533134-6.001236transcriptional regulator clcR
Bpet1534133-4.730063catechol 1,2-dioxygenase
Bpet1535131-5.008357muconate cycloisomerase I
Bpet1536232-5.465015putative secreted protein
Bpet1537233-5.307370carboxymethylenebutenolidase
Bpet1538032-5.332762maleylacetate reductase
Bpet1539031-5.432382AraC family transcriptional regulator
Bpet1540223-4.816350ring hydroxylating alpha subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1396DHBDHDRGNASE892e-23 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 89.3 bits (221), Expect = 2e-23
Identities = 68/257 (26%), Positives = 107/257 (41%), Gaps = 9/257 (3%)

Query: 3 KRFEGKVVIVTGAAQGIGRGVALRAAAEGGRVLFVD---RAEFVTEVAAEAPGGNTVGLI 59
K EGK+ +TGAAQGIG VA A++G + VD + +A +
Sbjct: 4 KGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 60 ADLETYEGARSAMAFAAEKFGGIDILINGVGGAIRMRPYAEFEPEQIDAEIRRSLMPTLY 119
AD+ A + G IDIL+N V G +R E+ +A +
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVN-VAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122

Query: 120 ACHAVLPHLLARGGGTIVNVSSNA--TRGIRRVPYSTAKGGVNALTQSLAMEYAPYNIRV 177
A +V +++ R G+IV V SN Y+++K T+ L +E A YNIR
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 178 VATAPGGTNAPPRRVPRNAAGDSQQEQAWMREAVQQVTESNFFKRYGSLDDQVGPILFMA 237
+PG T + + D + ++ +++ K+ D +LF+
Sbjct: 183 NIVSPGSTETD---MQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

Query: 238 SDEAAYITGTVLPVAGG 254
S +A +IT L V GG
Sbjct: 240 SGQAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1398BORPETOXINA270.035 Bordetella pertussis toxin A subunit signature.
		>BORPETOXINA#Bordetella pertussis toxin A subunit signature.

Length = 269

Score = 27.1 bits (59), Expect = 0.035
Identities = 14/37 (37%), Positives = 15/37 (40%)

Query: 18 REARHLDEREWEPWLDLYAPDAEYWMPAWDDDDQLTV 54
R R W WL + A A PAW DD TV
Sbjct: 5 RAIRQTARTGWLTWLAILAVTAPVTSPAWADDPPATV 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1431TCRTETA516e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 50.6 bits (121), Expect = 6e-09
Identities = 70/380 (18%), Positives = 128/380 (33%), Gaps = 65/380 (17%)

Query: 53 LAPSIAENFGLEVGSFAPVFAAGLFGLMVGALLLGPIADKIGRRWLVIAATFTFGLFTFL 112
+ + ++G+ + +A A +LG ++D+ GRR +++ + + +
Sbjct: 37 HSNDVTAHYGILLALYA-------LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI 89

Query: 113 TASASSINEFVILRFLTGLGLGGAMPNLTALATEYSPR----RYQGMIVAWLFAGIPIGA 168
A+A + I R + G+ G A + + R+ G + A G+ G
Sbjct: 90 MATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGP 148

Query: 169 IVGGLLSSWLLPFAGWQAAFHVGGVLPMLLALVLVFTLPESLRFLILKQDNPRRVLAIAN 228
++GGL+ + A F L L L F LPES + + P R A+
Sbjct: 149 VLGGLMGGF-----SPHAPFFAAAALNGLNFLTGCFLLPESHK----GERRPLRREAL-- 197

Query: 229 RIVPSGFPPEQQFSSPQKPVTGIPVRHLFTNGRWSGTLLLWIPYFMNLLIIYFI---ISW 285
P+ T L+ ++FI +
Sbjct: 198 -----------------NPLASFRWARGMTVVAA-------------LMAVFFIMQLVGQ 227

Query: 286 LPAML-------RQSGMPITAGIEAATAFSFGGAIGCLGTGKLLQMFGARKVALIEFVAT 338
+PA L R T GI A + TG + G R+ ++ +A
Sbjct: 228 VPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIAD 287

Query: 339 ILFILLLSTYSDAYWSVMLIAGFLGFTVQGAQAALNALVAGFYPTAIRSTGIGWALGIGR 398
+LL+ + W I L AL A+++ + G +
Sbjct: 288 GTGYILLAFATR-GWMAFPIMVLLAS-GGIGMPALQAMLSRQVDEERQGQLQGSLAALTS 345

Query: 399 VGSIIGPLIGGLMLSMHWQT 418
+ SI+GPL+ + + T
Sbjct: 346 LTSIVGPLLFTAIYAASITT 365


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1432SUBTILISIN260.041 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 26.3 bits (58), Expect = 0.041
Identities = 15/60 (25%), Positives = 25/60 (41%), Gaps = 4/60 (6%)

Query: 8 DILIVTKLDRHGRDAI-DISTTVRTLAEMGVRVYCLALGGADLTSSAGTMTMNVLNAVAQ 66
D+LI+ L++ G I + E V + ++LGG + + V AVA
Sbjct: 111 DLLIIKVLNKQGSGQYDWIIQGIYYAIEQKVDIISMSLGGPE---DVPELHEAVKKAVAS 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1435HTHTETR733e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 73.1 bits (179), Expect = 3e-18
Identities = 33/160 (20%), Positives = 59/160 (36%), Gaps = 2/160 (1%)

Query: 12 AVIDAAMDVFWTNGFEASSTQELCERTGLGRGSLYHAFGSKQNLYEQALRRYQE-LGLKA 70
++D A+ +F G ++S E+ + G+ RG++Y F K +L+ + + +G
Sbjct: 15 HILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELE 74

Query: 71 QTEILNGPGTAKERLQALLQWGVDGDLDPEKRRSCMA-LFSVMERGSKDPVIDQINRAYV 129
PG L+ +L ++ + E+RR M +F E + V+ Q R
Sbjct: 75 LEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNLC 134

Query: 130 NRLEAVICHVIAVGQRNGELADDRPALEVARAFLASYYGL 169
I + L D A GL
Sbjct: 135 LESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1436TCRTETA392e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 39.4 bits (92), Expect = 2e-05
Identities = 82/368 (22%), Positives = 130/368 (35%), Gaps = 36/368 (9%)

Query: 37 VTQIGYLISLYAIGMVVGGPLLTVGLLKLRVPNKQALLWLLGFYAVAQSVAASATSYDIM 96
G L++LYA+ P+L G L R + LL L AV ++ A+A ++
Sbjct: 42 TAHYGILLALYALMQFACAPVL--GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVL 99

Query: 97 AAARVATGVAGSACFGVSLAICAEIVGAESR----GRAASIVVGGLMLATVLGVPIATII 152
R+ G+ G A V+ A A+I + R G ++ G++ VLG +
Sbjct: 100 YIGRIVAGITG-ATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGF- 157

Query: 153 DQHWGWRASFWLVVALAVLCATVITFLVPRSKAAGTVSLGAELAEFKNRHLWAAYATSGL 212
A F+ AL L FL+P S L E WA T
Sbjct: 158 ----SPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVA 213

Query: 213 IIGATFAAFSYFAPILTEV--------TGFAAASIPWLLGVYGAANVVGNMVVGRYADKH 264
+ A F + + + A +I L +G + + ++
Sbjct: 214 ALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAAR 273

Query: 265 --TMPIMVWGLIVLGAALAVFSIFAQNQVLSLGALIVIGLVGV--PMNPAMIARVMKTAH 320
++ G+I G + FA ++ ++++ G+ P AM++R +
Sbjct: 274 LGERRALMLGMIADGTGY-ILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEER 332

Query: 321 PGAL--VNTVHTSVINIGLGVGAWVGGLGIAAGYGNRSPLWVGVALAVLGLLSL--LPYL 376
G L TS+ +I VG L A Y W G A L L LP L
Sbjct: 333 QGQLQGSLAALTSLTSI-------VGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPAL 385

Query: 377 GRKAASRA 384
R S A
Sbjct: 386 RRGLWSGA 393


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1442ARGREPRESSOR339e-04 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 33.3 bits (76), Expect = 9e-04
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 12/46 (26%)

Query: 168 SQSELARRLAADGYPVQQSHISRMVD---AVR---------YLLPA 201
+Q EL L DGY V Q+ +SR + V+ Y LPA
Sbjct: 21 TQDELVDILKKDGYNVTQATVSRDIKELHLVKVPTNNGSYKYSLPA 66


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1453HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1464HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1479IGASERPTASE280.028 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 28.5 bits (63), Expect = 0.028
Identities = 18/115 (15%), Positives = 43/115 (37%), Gaps = 4/115 (3%)

Query: 36 QTMNDQADQEQLASRLQRLEAQAAGLAETIEAIQQRPAV-ATAADLKDTRQILEARAAQV 94
+T+ + + QE +A A + + V A + + E + Q
Sbjct: 1038 ETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQT 1097

Query: 95 EKTLSSYAAADDLQALRVEVEQIK--ARQTAAPAPRAAAPARPRASGKAAAKPEP 147
+T + + +A +VE E+ + + T+ +P+ + + A + +P
Sbjct: 1098 TETKETATVEKEEKA-KVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP 1151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1492PF02370310.007 M protein repeat
		>PF02370#M protein repeat

Length = 168

Score = 30.8 bits (69), Expect = 0.007
Identities = 20/118 (16%), Positives = 39/118 (33%), Gaps = 9/118 (7%)

Query: 30 SSSAPPAADAGAKLTPEEMKALGIEGDTPRDTVATLVAQVKQLRTELQTVLSDNKSQREE 89
SS + + KL E L E + + + R ++ E
Sbjct: 2 SSISNVNTSSNGKLI-TEYNKLVEENSKLQKQLEEYLDSSDSKRENDP----QYRALMGE 56

Query: 90 NQRLRQRENSIDQRINSAL----ESERSNLRRDQQQAASERQQTEGLLADLQRRLESI 143
NQ LR+RE +I E + RR++ + + + + Q+ + +
Sbjct: 57 NQDLRKREGQYQDKIEELEKERKEKQERPERREKFERQHQDKHYQEQQKKHQQEQQQL 114


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1494HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1512DHBDHDRGNASE1211e-35 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 121 bits (305), Expect = 1e-35
Identities = 80/259 (30%), Positives = 124/259 (47%), Gaps = 10/259 (3%)

Query: 3 KRLENKVAFITGAAGGQGRAAAVVFAREGAKVAVVDVDEKGIEETARLVKETGGEAIAIR 62
K +E K+AFITGAA G G A A A +GA +A VD + + +E+ +K A A
Sbjct: 4 KGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 63 CDVSNEEQVKGAIQKTVDTFGKLTTLYNNAGIAHKNFMILAHELSVEEWEKIQNVNTKGM 122
DV + + + G + L N AG+ L H LS EEWE +VN+ G+
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLR---PGLIHSLSDEEWEATFSVNSTGV 120

Query: 123 FLVVKYGIPELLKAGGGTIINTSSTAGLINSPGGPSYTASKGAIISFTRHLAATYAKKGI 182
F + ++ G+I+ S + +Y +SK A + FT+ L A+ I
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 183 RANAIAPGYVITLMTKAM---EDLLPEVDKVASE----ATPLGRGAQPEEIANVALFLAS 235
R N ++PG T M ++ E+ +V K + E PL + A+P +IA+ LFL S
Sbjct: 181 RCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240

Query: 236 DESSFVTGAVIVADGGMTI 254
++ +T + DGG T+
Sbjct: 241 GQAGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1514cdtoxina290.043 Cytolethal distending toxin A signature.
		>cdtoxina#Cytolethal distending toxin A signature.

Length = 258

Score = 28.9 bits (64), Expect = 0.043
Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 1/37 (2%)

Query: 380 AASQPATVETAQPGAGVPGVAAPGNAATPTPEPEARP 416
+ P+ E P G PG A P N A P PEP P
Sbjct: 43 GPTVPSPDEPGLPLPG-PGPALPTNGAIPIPEPGTAP 78


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1529cdtoxina290.042 Cytolethal distending toxin A signature.
		>cdtoxina#Cytolethal distending toxin A signature.

Length = 258

Score = 28.9 bits (64), Expect = 0.042
Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 1/37 (2%)

Query: 380 AASQPATVETAQPGAGVPGVAAPGNAATPTPEPEARP 416
+ P+ E P G PG A P N A P PEP P
Sbjct: 43 GPTVPSPDEPGLPLPG-PGPALPTNGAIPIPEPGTAP 78


20Bpet1552Bpet1564Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1552315-1.285157heat-inducible transcription repressor
Bpet1553415-1.073840ferrochelatase
Bpet1554618-1.583779hypothetical protein
Bpet1555415-0.502375heat shock protein GrpE
Bpet1556315-0.566341putative thioredoxin
Bpet1557215-0.792776molecular chaperone DnaK
Bpet1558113-0.150487chaperone protein DnaJ
Bpet1559114-0.274273hypothetical protein
Bpet1560013-0.140440putative zinc protease
Bpet1561-215-1.149963acetyltransferase
Bpet1562-119-2.366470hypothetical protein
Bpet1563121-2.521211putative ATP-binding protein
Bpet1564223-2.471076putative cytochrome c
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1555IGASERPTASE290.010 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 29.3 bits (65), Expect = 0.010
Identities = 25/115 (21%), Positives = 46/115 (40%), Gaps = 6/115 (5%)

Query: 8 VDQAPESNEPAPAVPA-----TVEALQAELAAVRAELEAAQATVAGQQEQVLRARADAEN 62
VD+AP PAPA P+ E + E V + A T A +E A+++ +
Sbjct: 1020 VDEAPVP-PPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKA 1078

Query: 63 VRRRAQEDVSKARKFGIESFAESLVPVKDSLEAALAQPDQTLEALREGVEVTLKQ 117
+ + S + ++ + E A + ++T E + +V+ KQ
Sbjct: 1079 NTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQ 1133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1557SHAPEPROTEIN1414e-39 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 141 bits (356), Expect = 4e-39
Identities = 86/396 (21%), Positives = 142/396 (35%), Gaps = 93/396 (23%)

Query: 2 SKIIGIDLGTTNSCVAVMDGGQVKIIENAEGART----TPSIVAYMDDGETLVGAPAKRQ 57
S + IDLGT N+ + V G V + R +P VA VG AK+
Sbjct: 10 SNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVA-------AVGHDAKQM 62

Query: 58 AVTNPKNTLYAVKRLIGRKFDEKAVQKDIDLMPYSIVKADNGDAWVEARGKKIAPPQVSA 117
P N + A++ + + IA V+
Sbjct: 63 LGRTPGN-IAAIRPM---------------------------------KDGVIADFFVTE 88

Query: 118 DVLRK-MKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAA 176
+L+ +K+ + ++ VP +R+A +++ + AG +I EP AAA
Sbjct: 89 KMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAA 148

Query: 177 LAFGLDKSEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQR 236
+ GL SE V D+GGGT +V++I + V + +GG+ FD+
Sbjct: 149 IGAGLPVSE--ATGSMVVDIGGGTTEVAVISLNGV---------VYSSSVRIGGDRFDEA 197

Query: 237 IIDYIIGEFKKEQGVDLSKDVLALQRLKEAAEKAKIELSSS----QQTEINLPYITADAS 292
II+Y+ + G + AE+ K E+ S+ + EI +
Sbjct: 198 IINYVRRNYGSLIG-------------EATAERIKHEIGSAYPGDEVREIEVRGRNLAEG 244

Query: 293 GPKHLNLKITRAKLEALVEEL----------IERTIDPCRVAIKDAGVKVSEIDDVILVG 342
P+ L + LEAL E L +E+ I + G ++L G
Sbjct: 245 VPRGFTLN-SNEILEALQEPLTGIVSAVMVALEQCPPELASDISERG--------MVLTG 295

Query: 343 GMTRMPKVQEKVKEFFGKDPRKDVNPDEAVAAGAAI 378
G + + + E G +P VA G
Sbjct: 296 GGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGK 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1561SACTRNSFRASE389e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 37.6 bits (87), Expect = 9e-06
Identities = 13/60 (21%), Positives = 25/60 (41%)

Query: 92 NKYTVEHSVYIDARFRGRGLAEALMRTLIARARERQLHVLVGGIDAANTASIRLHEKLGF 151
N Y + + + +R +G+ AL+ I A+E L+ N ++ + K F
Sbjct: 87 NGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1563HTHFIS290.031 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.031
Identities = 22/102 (21%), Positives = 33/102 (32%), Gaps = 8/102 (7%)

Query: 15 ALGKAENWAKD-ERRVCARLTKPCDCTIIVAEPCQPIARTAPRAPLLPPPMPAAQSPAAP 73
A K E+ L KP D T ++ I R P + P
Sbjct: 83 AQNTFMTAIKASEKGAYDYLPKPFDLTELIGI----IGRALAEPKRRPSKLEDDSQDGMP 138

Query: 74 RFDGTERYVATDDLKLAVNAALTLQRPLLIKGEPGTGKTMLA 115
+ + ++ T L+I GE GTGK ++A
Sbjct: 139 LVGRSAAM--QEIYRVLARLMQT-DLTLMITGESGTGKELVA 177


21Bpet1605Bpet1619Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1605225-1.304270acetolactate synthase 3 regulatory subunit
Bpet1606025-0.488020ketol-acid reductoisomerase
Bpet1607-1210.259769hypothetical protein
Bpet1608-1200.829058putative lipoprotein
Bpet1609-117-1.16754430S ribosomal protein S15
Bpet1610-112-1.830984polynucleotide phosphorylase/polyadenylase
Bpet1611-17-2.906722putative lipoprotein
Bpet1612-19-3.756973threonine dehydratase
Bpet1613014-5.786367hypothetical protein
Bpet1614117-6.885898hypothetical protein
Bpet1615222-7.292136hypothetical protein
Bpet1616122-7.348449hypothetical protein
Bpet1617121-6.543500hypothetical protein
Bpet1618119-6.529175putative secreted protein
Bpet1619-211-3.924689hypothetical protein
22Bpet1664Bpet1686Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet16642102.277200GntR family transcriptional regulator
Bpet16651112.4506113-isopropylmalate dehydratase large subunit
Bpet16661112.551139hypothetical protein
Bpet16671112.988359putative phosphorylmutase
Bpet16683123.597544cytochrome b561 family protein
Bpet16694124.139968transporter protein
Bpet16702133.974518hypothetical protein
Bpet16710162.123966DNA repair exonuclease
Bpet16720160.959187hypothetical protein
Bpet1673116-0.985819hypothetical protein
Bpet1674117-2.037072hypothetical protein
Bpet1675121-2.700277TetR family transcriptional regulator
Bpet1676326-3.581905outer membrane porin protein precursor
Bpet1677023-3.289870NADH-ubiquinone oxidoreductase chain A
Bpet1678-122-0.780874NADH dehydrogenase subunit B
Bpet1679-123-0.768813NADH dehydrogenase subunit C
Bpet1680-122-1.111374NADH dehydrogenase subunit D
Bpet1681-222-0.673545NADH dehydrogenase subunit E
Bpet1682-322-0.539154NADH dehydrogenase I, 51 kDa subunit, chain F
Bpet1683022-0.671397NADH dehydrogenase subunit G
Bpet1684321-2.793899NADH dehydrogenase subunit H
Bpet1685219-2.231231NADH dehydrogenase subunit I
Bpet1686219-2.037266NADH-ubiquinone oxidoreductase, chain J
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1669TCRTETA417e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 40.6 bits (95), Expect = 7e-06
Identities = 77/389 (19%), Positives = 147/389 (37%), Gaps = 20/389 (5%)

Query: 12 KIAVPAFGPSILYGISNGAILPVVALSARELDAS---VATSGLIVALIGIGSLVSNIPAA 68
+ + L + G I+PV+ R+L S A G+++AL +
Sbjct: 5 RPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLG 64

Query: 69 MITSRYGERLSMMGAAALSVLALLLCIFAGHAAVLAVGVFLIGMASSVFMLARQTYMIDA 128
++ R+G R ++ + A + + + A VL +G + G+ + +A Y+ D
Sbjct: 65 ALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVA-GAYIADI 123

Query: 129 VPAYMRARALSTLAGTMRIGVFVGPFAGAALIHFMGLQGAYWVAAVAMAGAGLIAHLAPD 188
RAR ++ G+ GP G + F + AA+ L P+
Sbjct: 124 TDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 189 MTPPERRDAAVQAKPRV----LDVARTHRRVFLTLGLGILLVSAVRASRQVVIPLWADHL 244
ERR +A + T + + + LV V A+ V+ D
Sbjct: 184 SHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG--EDRF 241

Query: 245 GINPTTTSI---IYGLVAAIDMSVFYPAGTLMDRRGRLWVAVPSTLLMGFALIGTSLTSG 301
+ TT I +G++ ++ ++ G + R G + + G I + +
Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMI--TGPVAARLGERRALMLGMIADGTGYILLAFATR 299

Query: 302 VIGFLIVSMMLGMGNGIGSGIVMTLGADAAPSTGRTEFLGIWRLVSDLGSSLGPVVLSAI 361
+ ++L G GIG + + + + + G ++ L S +GP++ +AI
Sbjct: 300 GWMAFPIMVLLASG-GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAI 358

Query: 362 TALVSLAAAVAAMGTFGLAGAAVFWRWLP 390
A A+ G +AGAA++ LP
Sbjct: 359 YA----ASITTWNGWAWIAGAALYLLCLP 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1675HTHTETR671e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 67.3 bits (164), Expect = 1e-15
Identities = 40/170 (23%), Positives = 66/170 (38%), Gaps = 7/170 (4%)

Query: 85 QRLTALRRQLILDAAQRVFERDGLEKTTVRAIAKEAGCTTGAIYPWFGGKEAIYAELLEA 144
++ RQ ILD A R+F + G+ T++ IAK AG T GAIY F K +++E+ E
Sbjct: 6 KQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWEL 65

Query: 145 SLERLRDRLASALAQGGG---GAARRVIEAFFGYYAERATEFSLGMYLFQ---GLGPRGL 198
S + + A+ G R ++ L +F +G +
Sbjct: 66 SESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAV 125

Query: 199 GREADDRLNARLRG-CVDLLGRGLRAAKPWPDEMVAVEQMQVFTYLMGLL 247
++A L L + A D M + + Y+ GL+
Sbjct: 126 VQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLM 175


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1676ECOLNEIPORIN971e-24 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 96.8 bits (241), Expect = 1e-24
Identities = 82/385 (21%), Positives = 138/385 (35%), Gaps = 59/385 (15%)

Query: 1 MKKTLLAAALLAGFAGVAQAETSVTLYGIIDTGIGYNKVKGAGFDGSRVGMING--VQNG 58
MKK+L+A L A A VTLYG I G+ ++ + V G
Sbjct: 1 MKKSLIALTLAAL---PVAAMADVTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLG 57

Query: 59 SRWGLRGTEDLGDGLQAVFQLESGFNSGNGNHAQDGRLFGRQATIGLQSDSWGRLDFGRQ 118
S+ G +G EDLG+GL+A++Q+E + + RQ+ IGL+ +G+L GR
Sbjct: 58 SKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGW----GNRQSFIGLKGG-FGKLRVGRL 112

Query: 119 TNIASKYFGSIDPFGAG---FGQANIGMGLSAMNTVRWDNMVMYQTPSYSGFQFGVGYSF 175
++ G I+P+ + G I + + +VR+D+ P ++G V Y+
Sbjct: 113 NSVLKDT-GDINPWDSKSDYLGVNKIAEPEARLISVRYDS------PEFAGLSGSVQYAL 165

Query: 176 SVDDNTTDDDRVGFRTADNVRGITTGLRYVNGPLNVALSYDQLNASNAQAQDEVDATPRS 235
+ + N G Y NG V Q ++ +
Sbjct: 166 NDNAG-----------RHNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIE-KYQI 213

Query: 236 YGIGASYDFEVVKVALAYARTTDGWFGGQGINGIGAVDSDADGVDDQFPLSSNRFADGFK 295
+ + + YD + + ++ + D + + V + RF +
Sbjct: 214 HRLVSGYDNDALYASV-AVQQQDAKLVEEN-----YSHNSQTEVAATL---AYRFGNVTP 264

Query: 296 SNSYMVGLTAPIGGASKLFGSWQMVDPSNDKLTGGEEKMNVFSLGYTYDLSKRTNLYAYG 355
SY G + +G YD SKRT+
Sbjct: 265 RVSYAHGFKGSFDAT------------------NYNNDYDQVVVGAEYDFSKRTSALVSA 306

Query: 356 SYAKNYAFIDDVKSTAVGVGIRHRF 380
+ + STA GVG+RH+F
Sbjct: 307 GWLQEGKGESKFVSTAGGVGLRHKF 331


23Bpet1836Bpet1843Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet18362124.294001hypothetical protein
Bpet18372124.200317hypothetical protein
Bpet18380114.165772hypothetical protein
Bpet18390113.249856tryptophanyl-tRNA synthetase
Bpet18400123.766732hypothetical protein
Bpet1841-1113.359713hypothetical protein
Bpet1842-1142.828909ArsR family transcriptional regulator
Bpet18430153.157507hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1838CHANLCOLICIN270.014 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 27.0 bits (59), Expect = 0.014
Identities = 11/49 (22%), Positives = 20/49 (40%)

Query: 59 WGWRYGTVEWIGILTLAGLLLIWLVSFRAGPALAVGGVCAVAAPVLAWI 107
W + T+E ++ L S AG L + G+ V + ++I
Sbjct: 460 WKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYI 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1841TCRTETB1147e-30 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 114 bits (287), Expect = 7e-30
Identities = 75/412 (18%), Positives = 160/412 (38%), Gaps = 15/412 (3%)

Query: 17 RQRQALAALCLAVLVAQVDTAAVNLATRAVGLHFHAGVQALQWVIDSYNLAYAALLLTGG 76
R Q L LC+ + ++ +N++ + F+ + WV ++ L ++ G
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 77 LLADLWGRRRVFLLGAGLFCAASLGCALAPSVAA-LVAARVGAGVGAALLIPASLALIRV 135
L+D G +R+ L G + C S+ + S + L+ AR G GAA PA + ++
Sbjct: 71 KLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAA-AFPALVMVVVA 129

Query: 136 GWPDPAARARVLGIWAACNGLALAVGPTLGGILMQHYGWPGIFLAAIPLGVAAMALAWRA 195
+ R + G+ + + VGP +GG++ + W +L IP+
Sbjct: 130 RYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMK 187

Query: 196 IPESSDPRGRRWDAGAQAMAAVALAALALAAIESHTTPWLAVVAATVAAMAVAGFIRIER 255
+ + +D + +V + L + ++ + ++ + F++ R
Sbjct: 188 LLKKEVRIKGHFDIKGIILMSVGIVFFMLFT---TSYSISFLIVSVLSFLI---FVKHIR 241

Query: 256 RAEAAALVPLSLFRSARFCGALAATSAMTFGMYGALFLLPLAWQDSGRFDAVQAGLALMP 315
+ V L ++ F + + + G + ++P +D + + G ++
Sbjct: 242 KVT-DPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIF 300

Query: 316 MALVFVLIS-PWSGLLCGRLGRRAMTAGGVAIIGAGLWLIALGAASAALAPALAGLAATG 374
+ V+I G+L R G + GV + + + + + +
Sbjct: 301 PGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFV-- 358

Query: 375 LGMGLATGPLMDTAV-SSVPAARAGTASALVNVARMVGATLGVALSGSLYTL 425
LG T ++ T V SS+ AG +L+N + G+A+ G L ++
Sbjct: 359 LGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSI 410


24Bpet1951Bpet1960Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1951-1133.276859putative inner membrane permease polyamine
Bpet1952-1113.585125hypothetical protein
Bpet1953-2113.0857513-methyladenine DNA glycosylase
Bpet1954-2112.734515hypothetical protein
Bpet1955-2113.459961hypothetical protein
Bpet1956-2133.822973putative acyl-CoA dehydrogenase
Bpet19570123.196258ribose-5-phosphate isomerase A
Bpet1958-2122.694080putative transport protein
Bpet1959-2142.762149hypothetical protein
Bpet1960-3163.140763acyl-CoA transferase/carnitine dedydratase
25Bpet1984Bpet1996Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1984083.194913putative L-proline 4-hydroxylase
Bpet1985084.1960812-keto-3-deoxygluconate permease
Bpet1986084.323137hypothetical protein
Bpet1987-382.572020putative 4-hydroxythreonine-4-phosphate
Bpet1988-3111.311770GntR family transcriptional regulator
Bpet1989-2110.949880hypothetical protein
Bpet1990-1111.328760sugE protein
Bpet1991-192.536864CDP-6-deoxy-delta-3,4-glucoseen reductase
Bpet1992-1112.996898D-amino acid dehydrogenase small subunit
Bpet1993-1113.322162glycerol-3-phosphate-binding periplasmic protein
Bpet19941134.531781putative lipoprotein
Bpet19951114.391938putative outer membrane lipoprotein
Bpet19962135.017292hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1995BCTERIALGSPD486e-08 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 48.0 bits (114), Expect = 6e-08
Identities = 58/296 (19%), Positives = 105/296 (35%), Gaps = 46/296 (15%)

Query: 256 SLVVTDIPDVLDRIGQFIERENQALTRRVRLLFEEI--TVVANDSAEGGIDWKAVYDSAR 313
+L+VT PDV++ + + I Q RR ++L E I V D GI W
Sbjct: 320 ALIVTAAPDVMNDLERVI---AQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAGMT 376

Query: 314 AAVAATLPVA-----------AGGAAAALGATVDS------GPFQGT-RAIVSALSQTGA 355
+ LP++ G +++L + + S G +QG +++ALS +
Sbjct: 377 QFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGNWAMLLTALSSSTK 436

Query: 356 VLRHSSVPVLTLNRRPVTHAVRTTFSYIDQVQSTAVPGIDAALGSTALPSVSISQKQETV 415
++ ++TL+ T V + Q+T S ++ +K TV
Sbjct: 437 NDILATPSIVTLDNMEATFNVGQEVPVLTGSQTT----------SGDNIFNTVERK--TV 484

Query: 416 GTFLTLVPDAQADGRILLSIAYDNTVAQPIKSVTFGTQGNQIQVQQITIDGNGTVQQVAL 475
G L + P +LL + Q + SV T + V +
Sbjct: 485 GIKLKVKPQINEGDSVLL------EIEQEVSSVADAASSTS-SDLGATFNTRTVNNAVLV 537

Query: 476 SPGQPVILSGF--DRRQDEYDRRRLSADAPLLAG--GQDRASSERLTTVVLVTAQV 527
G+ V++ G D D+ L D P++ + ++ + V
Sbjct: 538 GSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTV 593



Score = 30.3 bits (68), Expect = 0.021
Identities = 16/63 (25%), Positives = 24/63 (38%), Gaps = 6/63 (9%)

Query: 229 ALQAVRVRIL-----PFLTQAGTIADLDGGGS-SLVVTDIPDVLDRIGQFIERENQALTR 282
L V R L AG + + S L++T V+ R+ +ER + A R
Sbjct: 133 PLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR 192

Query: 283 RVR 285
V
Sbjct: 193 SVV 195


26Bpet2071Bpet2093Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet20713193.186000entericidin B-like bacteriolytic toxin
Bpet20721173.183021putative prepilin protein
Bpet20731162.597659hypothetical protein
Bpet20743152.842477putative secreted protein
Bpet20755143.280287putative secreted protein
Bpet20764143.414381putative secreted protein
Bpet20772122.365833putative outer membrane protein
Bpet20783122.494477putative general secretion pathway ATPase
Bpet2079292.344233hypothetical protein
Bpet20800130.976197hypothetical protein
Bpet2081-212-1.089552hypothetical protein
Bpet2082-314-2.799648putative methyltransferase
Bpet2083-216-3.511133hypothetical protein
Bpet2084-216-3.565042inosine-5'-monophosphate dehydrogenase
Bpet2085-223-4.048059GMP synthase
Bpet2086-337-4.533894hypothetical protein
Bpet2087-333-3.713036hypothetical protein
Bpet2088030-2.939691AraC family transcriptional regulator
Bpet2089-128-2.333094LysR family transcriptional regulator
Bpet2090020-3.395023quinone oxidoreductase
Bpet2091113-3.178047LysR family transcriptional regulator
Bpet2092114-3.716502NAD(P)H dehydrogenase, quinone 1
Bpet2093214-2.922542transposase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2077BCTERIALGSPD1501e-41 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 150 bits (380), Expect = 1e-41
Identities = 68/253 (26%), Positives = 113/253 (44%), Gaps = 20/253 (7%)

Query: 161 NMVLLDVQVVEIPSARLREFGLQWDALSQGGLHAGGV-WQPGSSLQLAD---------AA 210
VL++ + E+ A G+QW + G +++ A+ ++
Sbjct: 345 PQVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSS 404

Query: 211 QPPALSMQGMGAAGYFGVNALLSARLAALAQRGEAVMLAQPQLLARSGTTASFLAGGEVP 270
ALS AAG++ N + L AL+ + +LA P ++ A+F G EVP
Sbjct: 405 LASALSSFNGIAAGFYQGN--WAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVP 462

Query: 271 Y---STTDAQGNS--STEFKPYGVSLNITPRIDRNGAIRSRIEVEASSIDTSLSVAG--- 322
S T + N + E K G+ L + P+I+ ++ IE E SS+ + S
Sbjct: 463 VLTGSQTTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDL 522

Query: 323 GPALRTRRAVTEFNVRSGQTLVLGGFLSRERSHERSGLPVLQDIPLLGALFSSRRDQHKE 382
G TR V SG+T+V+GG L + S +P+L DIP++GALF S + +
Sbjct: 523 GATFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSK 582

Query: 383 TELAIFVTPRIVS 395
L +F+ P ++
Sbjct: 583 RNLMLFIRPTVIR 595


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2079BCTERIALGSPF320.002 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 32.1 bits (73), Expect = 0.002
Identities = 33/142 (23%), Positives = 58/142 (40%), Gaps = 12/142 (8%)

Query: 97 RLRGRRLARFEQQLPGALLALASALRAGVGVSTALRHIVDHSEPPLAQEFGLMLREQRLG 156
RL LA +QL A+ + A + + AL + SE P ++ R
Sbjct: 64 RLSTSDLALLTRQL-------ATLVAASMPLEEALDAVAKQSEKP---HLSQLMAAVRSK 113

Query: 157 VSFDAALARLSQRVPSEASALVAAALRVATHTGGNLAETLDGIARTLRERLQLQGKVR-A 215
V +LA + P L A + A T G+L L+ +A +R Q++ +++ A
Sbjct: 114 VMEGHSLADAMKCFPGSFERLYCAMVA-AGETSGHLDAVLNRLADYTEQRQQMRSRIQQA 172

Query: 216 LTAQGRLQAWIVGALPLLLAAV 237
+ L + + +LL+ V
Sbjct: 173 MIYPCVLTVVAIAVVSILLSVV 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2080BCTERIALGSPF320.002 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 32.1 bits (73), Expect = 0.002
Identities = 13/50 (26%), Positives = 23/50 (46%)

Query: 192 MRAGMPRAAALKALADRADSPAVRSWIAALTQADSLGMSLGAVLRGHAAQ 241
+ A MP AL A+A +++ P + +AA+ G SL ++
Sbjct: 81 VAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLADAMKCFPGS 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2084HTHFIS310.013 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.013
Identities = 13/70 (18%), Positives = 25/70 (35%), Gaps = 5/70 (7%)

Query: 217 RVGAAVGVGAGTEERVEKLAAAGVDVIIVDTAHGHSAGVLERVRWVKQNYPKVEVI---- 272
R G V + + +AA D+++ D + + +K+ P + V+
Sbjct: 25 RAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA-FDLLPRIKKARPDLPVLVMSA 83

Query: 273 GGNIATAAAA 282
TA A
Sbjct: 84 QNTFMTAIKA 93


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet208660KDINNERMP280.009 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 28.4 bits (63), Expect = 0.009
Identities = 12/76 (15%), Positives = 28/76 (36%), Gaps = 6/76 (7%)

Query: 7 PYLVAAHVTAVVFLVGGLLAQERMVNAISQSPPQEQIGMLAALLRFDRLVTTPA-LLLTW 65
PY + + + + ++M P Q++I ++ + P+ L+L +
Sbjct: 463 PYYILP-----ILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYY 517

Query: 66 IFGLSLALSAGWLSSR 81
I + + L R
Sbjct: 518 IVSNLVTIIQQQLIYR 533


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2091PF05043290.022 Transcriptional activator
		>PF05043#Transcriptional activator

Length = 493

Score = 29.1 bits (65), Expect = 0.022
Identities = 14/53 (26%), Positives = 25/53 (47%)

Query: 8 LNLLVTLEALLAEQNVTRAAERLHLSQPAVSTQLSRLRTLFDDPLLIPTQRGM 60
L LL L + + AE L+ ++ AV LS +++ F D + + G+
Sbjct: 13 LELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNGI 65


27Bpet2135Bpet2151Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2135215-0.524232flagellar biosynthesis protein FliR
Bpet2136115-0.170473flagellar biosynthesis protein FliQ
Bpet2137-2123.503170flagellar biosynthesis protein FliP
Bpet2138-2124.279429flagellar biosynthesis protein FliO
Bpet2139-2114.140149flagellar motor switch protein FliN
Bpet2140-1124.415109flagellar motor switch protein FliM
Bpet21410124.628271flagellar basal body-associated protein FliL
Bpet2142-1124.455388flagellar hook-length control protein FliK
Bpet2143-1152.355444flagellar biosynthesis chaperone FliJ
Bpet2144-1142.455605flagellar biosynthesis ATPase FliI
Bpet2145-1141.614968flagellar assembly protein H
Bpet2146-2102.869942flagellar motor switch protein G
Bpet2147-293.955060flagellar MS-ring protein
Bpet2148-1114.276696flagellar hook-basal body complex protein FliE
Bpet2149-2142.944200hypothetical protein
Bpet2150-2142.886354hypothetical protein
Bpet2151-1153.120915hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2135TYPE3IMRPROT1681e-53 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 168 bits (428), Expect = 1e-53
Identities = 123/256 (48%), Positives = 183/256 (71%), Gaps = 1/256 (0%)

Query: 1 MINFTQQQLDAWLLQFLWPFVRMLALVGSAPLFSESTIPIRIKVALAFMLTVAVAPGLEP 60
M+ T +Q +WL + WP +R+LAL+ +AP+ SE ++P R+K+ LA M+T A+AP L
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 PPAIPPGSYAGLWLLGQQVLIGIAMGFTMRIVFAAVQTAGEFVGLQMGLSFASFFDPSTG 120
P S+ LWL QQ+LIGIA+GFTM+ FAAV+TAGE +GLQMGLSFA+F DP++
Sbjct: 61 NDV-PVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASH 119

Query: 121 ANTAVLSRLLNIVAMLVFLALDGHLLVLAALVRSFDVLPLTQLTLDPNGWGILVQWGQTI 180
N VL+R+++++A+L+FL +GHL +++ LV +F LP+ L+ N + L + G I
Sbjct: 120 LNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLI 179

Query: 181 FVSGLLLALPLICALLTINLAMGILNRAAPQLSVFAVGFPVSLITGLLLLAAVLPHAAPF 240
F++GL+LALPLI LLT+NLA+G+LNR APQLS+F +GFP++L G+ L+AA++P APF
Sbjct: 180 FLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPF 239

Query: 241 LEGLMRDGLQAISDVL 256
E L + ++D++
Sbjct: 240 CEHLFSEIFNLLADII 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2136TYPE3IMQPROT591e-15 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 59.4 bits (144), Expect = 1e-15
Identities = 26/78 (33%), Positives = 44/78 (56%)

Query: 4 ETVMSMTYQALKIALAMAGPLLLVTLAVGLVIAVFQAATQINEMTLSFIPKLLAMCGVLV 63
+ ++ +AL + L ++G +V +GL++ +FQ TQ+ E TL F KLL +C L
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 LMGPWLLGLMTDYIRQLI 81
L+ W ++ Y RQ+I
Sbjct: 62 LLSGWYGEVLLSYGRQVI 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2137FLGBIOSNFLIP2837e-99 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 283 bits (725), Expect = 7e-99
Identities = 162/243 (66%), Positives = 190/243 (78%), Gaps = 2/243 (0%)

Query: 19 LAAAALAGLALFPAGVVAQATLPALTATPGPGGAQTYSLSMQTLLLMTSLSFLPAALLMM 78
L + A L L AQ LP +T+ P PGG Q++SL +QTL+ +TSL+F+PA LLMM
Sbjct: 4 LLSVAPVLLWLITPLAFAQ--LPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMM 61

Query: 79 TGFTRIIIVLGLLRSALGTAMSPPNHVLIGLALFLTFYTMSPVFDRIYSEAYKPLSEGSI 138
T FTRIIIV GLLR+ALGT +PPN VL+GLALFLTF+ MSPV D+IY +AY+P SE I
Sbjct: 62 TSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKI 121

Query: 139 PFETAVERAAAPLHTFMLHQTRENDLTLFANLANQPALEDPSQVPMKILVPAFITSELKT 198
+ A+E+ A PL FML QTRE DL LFA LAN L+ P VPM+IL+PA++TSELKT
Sbjct: 122 SMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKT 181

Query: 199 AFQIGFTIFIPFLIIDLVVASVLMALGMMMVPPVTVALPFKLMLFVLADGWNLLLGSLAS 258
AFQIGFTIFIPFLIIDLV+ASVLMALGMMMVPP T+ALPFKLMLFVL DGW LL+GSLA
Sbjct: 182 AFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQ 241

Query: 259 SFY 261
SFY
Sbjct: 242 SFY 244


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2139FLGMOTORFLIN1365e-44 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 136 bits (345), Expect = 5e-44
Identities = 78/133 (58%), Positives = 94/133 (70%), Gaps = 13/133 (9%)

Query: 46 DDWAGAMAEQASAASTAPAAAAPAAAPAARPAGGSVFKPLADAAGGNGNDIDLIMDVPVQ 105
D WA A+ EQ + + + A A GG V + D IDLIMD+PV+
Sbjct: 17 DLWADALNEQKATTTKSAADAVFQQL-----GGGDVSGAMQD--------IDLIMDIPVK 63

Query: 106 LTVELGRTRLTIKNLLQLGQGSVVELDGLAGEPMDIFVNGYLIAQGEVVVVEEKYGIRLT 165
LTVELGRTR+TIK LL+L QGSVV LDGLAGEP+DI +NGYLIAQGEVVVV +KYG+R+T
Sbjct: 64 LTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRIT 123

Query: 166 DIITPSERINRLN 178
DIITPSER+ RL+
Sbjct: 124 DIITPSERMRRLS 136


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2140FLGMOTORFLIM2782e-94 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 278 bits (712), Expect = 2e-94
Identities = 97/318 (30%), Positives = 165/318 (51%), Gaps = 8/318 (2%)

Query: 7 LSQDEVDALLAGV-TGESDSE-SRDEADARGARAYDLSSPDRVVRRRMQTLELINERFAR 64
LSQDE+D LL + +G++ E +R +D R YD PD+ + +M+TL L++E FAR
Sbjct: 5 LSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFAR 64

Query: 65 QLRHLLLNFMRRNADITVGSIKILKYADFERNLPVPSNLNMIQMKPLRGTALFTYDPSLV 124
L +R + V S+ L Y +F R++P PS L +I M PL+G A+ DPS+
Sbjct: 65 LTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPSIT 124

Query: 125 FLVIDSLFGGDGRYHTRVEGRDFTTTEQRIIRRLLNLTLESYGKSWEAVYPIEFEYVRSE 184
F +ID LFGG G+ RD T E ++ ++ L + +SW V + + E
Sbjct: 125 FSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQIE 182

Query: 185 MHTKFASITGNNEVVVVSSFHIEFGATGGDLNICLPYSMIEPVRDLL-TRPLQETTLEEV 243
+ +FA I +E+VV+ + + G G +N C+PY IEP+ L ++ +
Sbjct: 183 TNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRRSS 242

Query: 244 DQRWTHQLSRQVRSADVDLTAEFASIPSSIRELLRLKVGDVLPIE---VPETVIANVNGV 300
++ L ++ + D+D+ AE S+ S+R++L L+VGD++ + V + + ++
Sbjct: 243 TTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIGNR 302

Query: 301 PLMECSYGVFNGQYALRV 318
C GV + A ++
Sbjct: 303 KKFLCQPGVVGKKIAAQI 320


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2142FLGHOOKFLIK577e-11 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 56.8 bits (136), Expect = 7e-11
Identities = 72/280 (25%), Positives = 99/280 (35%), Gaps = 10/280 (3%)

Query: 173 PPAAALALSAAAPANTPAPQTQAPAAARPDTRVPGHELRGAPAPMPNPNAVAVTAVAEAP 232
P A ++ AA A+ + + D L N V P
Sbjct: 97 PLTTAQTMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLP 156

Query: 233 EHLNAQAAAEAELALQAASAVAQPAGHGAASSHAADAAASLAAAASPQATAPAPMPQAGA 292
L A P + A S A S + A
Sbjct: 157 TEKPTLFTKLTSEQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIT 216

Query: 293 LSLAVATPVAATPAWGADLGRQLVVLSHD------ATRGQHTAELRLDPPDLGPLRVTLS 346
P A P A LG S +GQ +AELRL P DLG ++++L
Sbjct: 217 PHQTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK 276

Query: 347 VNDGVASASFVSAHAAVRHAVEAALPQLHQALAQAGLSLGQANVGEH---GSQSGFDMQQ 403
V+D A VS H VR A+EAALP L LA++G+ LGQ+N+ G Q QQ
Sbjct: 277 VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQ 336

Query: 404 QAQGGGHGQGGGTQGDGAVALAPAAATRVARGDGLVDTFA 443
Q+Q + + + D + P + G+ VD FA
Sbjct: 337 QSQRTANHEPLAGEDDDTLP-VPVSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2143FLGFLIJ673e-17 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 67.1 bits (163), Expect = 3e-17
Identities = 51/145 (35%), Positives = 76/145 (52%)

Query: 1 MPSQLPLDTLIGLARESTDEAARALGRLNAERSHAERQLSMLQDYRQDYLLRLQNAMQTG 60
M L TL LA + ++AAR LG + AE QL ML DY+ +Y L + M G
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 MSAADCHNYQRFIATLDDAIGQQAAVLRQADSHLAQGRVHWQQQQRRLNSFDALAERERR 120
+++ NYQ+FI TL+ AI Q L Q + W+++++RL ++ L ER+
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 AQAVLETRREQRASDEFASRMMFRQ 145
A + E R +Q+ DEFA R R+
Sbjct: 121 AALLAENRLDQKKMDEFAQRAAMRK 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2145FLGFLIH912e-24 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 91.4 bits (226), Expect = 2e-24
Identities = 60/219 (27%), Positives = 105/219 (47%), Gaps = 6/219 (2%)

Query: 23 WRRWQMSSFDLPVEDAIEIVAPPPEPDPGPDPEELLREARAQAEAAGRREGLQQGREQGL 82
W+ W P + + IV P E + E L + AQ + + +QG + G+
Sbjct: 7 WKTWTPDDLAPPQAEFVPIVEP--EETIIEEAEPSLEQQLAQLQM----QAHEQGYQAGI 60

Query: 83 REGRQTGHAEGLAAGREAGYQEGLTQGREQARQEALQLHALAESCGASLADLEARMGQAL 142
EGRQ GH +G G G ++GL + + Q ++ L +L L++ + L
Sbjct: 61 AEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRL 120

Query: 143 LTLALDIAGQILRTTLAEQPESMLAAVREVLHINPAATGAMRLWVHPADLELVRQHLADE 202
+ +AL+ A Q++ T +++ ++++L P +G +L VHP DL+ V L
Sbjct: 121 MQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT 180

Query: 203 LREGHWRVLADESIARGGCRAETPYGDIDATLQTRWRRI 241
L WR+ D ++ GGC+ GD+DA++ TRW+ +
Sbjct: 181 LSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2146FLGMOTORFLIG295e-101 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 295 bits (757), Expect = e-101
Identities = 114/333 (34%), Positives = 189/333 (56%), Gaps = 2/333 (0%)

Query: 2 KNDGKPLDGVTRSAVLMMSLGEDAAAEVFKYLSAREVQLVGGSMANLKQVTRGDVAVVLE 61
D L G ++A+L++S+G + +++VFKYLS E++ + +A L+ +T VL
Sbjct: 9 ILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLL 68

Query: 62 EFRQEADQFMAVTLGSDDYIRTVLTKALGSDRAAGLIEDILEAGEGASGIDALNWLDPHT 121
EF++ + G DY R +L K+LG+ +A +I + L + + + + DP
Sbjct: 69 EFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINN-LGSALQSRPFEFVRRADPAN 127

Query: 122 VAELIGDEHPQIIATILVHLERDRAAGVLALLTDRLRNDVMLRIATFGGVQPAALSELTD 181
+ I EHPQ IA IL +L+ +A+ +L+ L ++ +V RIA P + E+
Sbjct: 128 ILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVER 187

Query: 182 VLNSVLAGQGA-KRSKMGGVRTAAEILNMMSSAEEEAVVESLRERDSDLAQKIIDEMFVF 240
VL LA + + GGV EI+NM E+ ++ESL E D +LA++I +MFVF
Sbjct: 188 VLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMFVF 247

Query: 241 DNLIDVEDRALQLILKEIDNDSLMVALKGASEELRNKFLRNMSSRAADILREDLEAQGPI 300
++++ ++DR++Q +L+EID L ALK ++ K +NMS RAA +L+ED+E GP
Sbjct: 248 EDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLGPT 307

Query: 301 RMSKVESEQKKILQIARRLAESGQIVLGNQGDD 333
R VE Q+KI+ + R+L E G+IV+ G++
Sbjct: 308 RRKDVEESQQKIVSLIRKLEEQGEIVISRGGEE 340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2147FLGMRINGFLIF452e-156 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 452 bits (1165), Expect = e-156
Identities = 244/555 (43%), Positives = 351/555 (63%), Gaps = 24/555 (4%)

Query: 18 LEKVRALPKPVLLGVAAALVAIVAVLAMWGREPDYKVLFANLDDRDGGAIVSALGQMNVP 77
L ++RA P+ L+ +A VAIV + +W + PDY+ LF+NL D+DGGAIV+ L QMN+P
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 78 YRFSGDGRALLVPADRVYATRMQLAGQGLPRGGSVGFELLDNARFGASQFAEQINYQRGL 137
YRF+ A+ VPAD+V+ R++LA QGLP+GG+VGFELLD +FG SQF+EQ+NYQR L
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRAL 135

Query: 138 EGELARSIEAMNTVQSARVHLALPRQSLFVRDRQAPTASVLLHLYPGRSLGDAQVAAVAW 197
EGELAR+IE + V+SARVHLA+P+ SLFVR++++P+ASV + L PGR+L + Q++AV
Sbjct: 136 EGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVH 195

Query: 198 LVASSVPDLTAENISIVDQNGRLLSAPLGEGRGLDADQSRLRRDIEQRTVERILTILNPL 257
LV+S+V L N+++VDQ+G LL+ GR L+ Q + D+E R RI IL+P+
Sbjct: 196 LVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDVESRIQRRIEAILSPI 255

Query: 258 VGPGNVQAQASAEMDFARREQTSEVYRPNQEPGQAAVRSKQTSDSLQTGIDPAQGVPGAL 317
VG GNV AQ +A++DFA +EQT E Y PN + +A +RS+Q + S Q G GVPGAL
Sbjct: 256 VGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGAL 315

Query: 318 SNQPPAAAQAPIVNPPAAPQAAQGGQPGQLAQAGQNAAQGAATQAAPRLPTNNRNDATIN 377
SNQP +API PP Q AQ + +A P + + + T N
Sbjct: 316 SNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAG-----------PRSTQRNETSN 364

Query: 378 YEVDRTISHVKQPVGMLKRLSVAVVVNYLPDSSGEPQPLPEEELTKLTNLVREAMGYSEA 437
YEVDRTI H K VG ++RLSVAVVVNY + G+P PL +++ ++ +L REAMG+S+
Sbjct: 365 YEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAMGFSDK 424

Query: 438 RGDSLNLVNSQFN---DKPVKPPFWRDPELLDLVKTVLAWVFGLALALWLYRR-LRPAVS 493
RGD+LN+VNS F+ + + PFW+ +D + W+ L +A L+R+ +RP ++
Sbjct: 425 RGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVRPQLT 484

Query: 494 NYL-NPPVDPEEAEARRQEMQREAQAAA--------RAKEVNRYEDNLQRARDMATKDPR 544
+ E+A+ R++ + + RA + E QR R+M+ DPR
Sbjct: 485 RRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPR 544

Query: 545 AVAMVMRAWMTQDEK 559
VA+V+R WM+ D +
Sbjct: 545 VVALVIRQWMSNDHE 559


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2148FLGHOOKFLIE618e-16 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 61.2 bits (148), Expect = 8e-16
Identities = 49/108 (45%), Positives = 72/108 (66%), Gaps = 6/108 (5%)

Query: 4 SGLSGIESMLQQMRAVVQAAQSNGVSPAELAPQPA-SFAAELQRSLQRVSAAQIAATNQG 62
S + GIE ++ Q++A +A++ E PQP SFA +L +L R+S Q AA Q
Sbjct: 1 SAIQGIEGVISQLQATAMSARAQ-----ESLPQPTISFAGQLHAALDRISDTQTAARTQA 55

Query: 63 KAYELGAPGVSLNDVMIDLQKSSIAFQTAVQVRNRLVAAYKEISAMSV 110
+ + LG PGV+LNDVM D+QK+S++ Q +QVRN+LVAAY+E+ +M V
Sbjct: 56 EKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2150TYPE3IMSPROT769e-20 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 75.6 bits (186), Expect = 9e-20
Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 2/79 (2%)

Query: 16 AVALSYGEHDT-APRVVAKGYGQIADTIVRTAREHGLYVHESRELV-SLLMQVDLDAHIP 73
A+ + Y +T P V K T+ + A E G+ + + L +L +D +IP
Sbjct: 268 AIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDHYIP 327

Query: 74 PQLYAAVAELLAWLYRLET 92
+ A AE+L WL R
Sbjct: 328 AEQIEATAEVLRWLERQNI 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2151PF03544310.010 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 30.7 bits (69), Expect = 0.010
Identities = 21/93 (22%), Positives = 27/93 (29%), Gaps = 2/93 (2%)

Query: 119 QAPPAQGKAPLWQPAPPPGPSAAAPDAGNSVRPAPAAA--GTASSSAANPATDPAAASSR 176
Q PP P +P P P P AP +P P P +
Sbjct: 67 QPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPA 126

Query: 177 SPATGGRPALPAGAGAQATQATQATHAGAPALP 209
SP PA P + A A + T +
Sbjct: 127 SPFENTAPARPTSSTATAATSKPVTSVASGPRA 159


28Bpet2164Bpet2206Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2164-314-4.439959amidophosphoribosyltransferase
Bpet2165-125-6.757974disulfide bond formation protein B
Bpet2166432-10.217458hypothetical protein
Bpet2167638-11.758674hypothetical protein
Bpet2168638-11.029533putative DNA-binding protein
Bpet2169535-10.455958hypothetical protein
Bpet2170528-8.648957hypothetical protein
Bpet2171420-5.172995hypothetical protein
Bpet2172014-1.407678DNA repair protein radC-like protein
Bpet2173-113-1.652237putative transposon
Bpet2174014-2.901371hypothetical protein
Bpet2175113-1.840730hypothetical protein
Bpet2176113-1.393425ParB-like nuclease
Bpet2177317-3.483169hypothetical protein
Bpet2178119-2.317225hypothetical protein
Bpet2179218-1.410743hypothetical protein
Bpet21803161.303332hypothetical protein
Bpet21814162.232444transcriptional regulator
Bpet21823162.174644lipoprotein
Bpet21832143.112529hypothetical protein
Bpet21843163.505203hypothetical protein
Bpet21851153.933548replication initiator/transcription repressor
Bpet21860153.010868putative partition protein
Bpet2187-1151.546497hypothetical protein
Bpet2188-1161.083846transposon
Bpet2189-2170.229237conjugal transfer protein TraF
Bpet2190-219-0.647301conjugal transfer protein VirD2
Bpet2191-128-2.9760593-ketoacyl-CoA thiolase
Bpet2192-130-3.640497oxidoreductase
Bpet2193-133-3.528295hypothetical protein
Bpet2194-134-3.473426hypothetical protein
Bpet2195140-5.747280hypothetical protein
Bpet2196139-5.780564MarR family transcriptional regulator
Bpet2197039-6.721176putative transmembrane transport protein
Bpetpseudo_08140-7.100046hypothetical protein
Bpetpseudo_09141-7.340027hypothetical protein
Bpet2198139-7.291082LysR family transcriptional regulator
Bpet2199027-4.379159putative threonine aldolase
Bpet2200024-3.588565tautomerase
Bpet2201022-1.785025MFS permease
Bpet2202219-0.632037LysR family transcriptional regulator
Bpet22032170.972579putative lipoprotein
Bpet22043151.027603conjugal transfer coupling protein TraG
Bpet22053160.775987putative DNA-binding protein
Bpet22063160.970524conjugal transfer protein TrbB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2171GPOSANCHOR320.007 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 32.0 bits (72), Expect = 0.007
Identities = 43/247 (17%), Positives = 88/247 (35%), Gaps = 25/247 (10%)

Query: 191 DAKLIADHYAKEHELAKKQETAQTIKNELGGSVEDISKIEG-ILLLKQKDA---EKKQVL 246
L A+ A A ++ + N I +E L+ + A + +
Sbjct: 213 IKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGA 272

Query: 247 LDAFDFRAQDKDRTKKVVDEVDERIAALNSERYSLSQNRKKVQASLQEDQILFSPDEAQR 306
++ + + ++ A L + L+ NR+ ++ L +A R
Sbjct: 273 MNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL----------DASR 322

Query: 307 LFGEAGVLFQGQIKKDFQQLIAFNRAITDERRGYLQEEFAEIEGELKRVNAELNALGKKR 366
EA + Q++ + Q+L N+ R+ L+ + K++ AE L +
Sbjct: 323 ---EA----KKQLEAEHQKLEEQNKISEASRQS-LRRDLDASREAKKQLEAEHQKL--EE 372

Query: 367 SEMLSFLSGTDVFTKYKQLSDEMVTLRADITSLEHQKGHLHRLQELRAEIRSLTE-ERDH 425
+S S + + + + + L +L + E + LTE E+
Sbjct: 373 QNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAE 432

Query: 426 LQARIEA 432
LQA++EA
Sbjct: 433 LQAKLEA 439


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2197TCRTETA759e-17 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 74.9 bits (184), Expect = 9e-17
Identities = 76/318 (23%), Positives = 127/318 (39%), Gaps = 17/318 (5%)

Query: 49 LFLLAGLAALGALATNIILPAFPDMAVALGTSVIDLSATLSSFLVAFAVGQLFVGP---- 104
L ++ AL A+ +I+P P + L S D++A L +A+ Q P
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSN-DVTAHYGILLALYALMQFACAPVLGA 65

Query: 105 LSDRFGRRWLVLAGLLAFVIGSAICAFASTLPQLIGGRVVQALGVCATSVLSRAIARDLF 164
LSDRFGRR ++L L + AI A A L L GR+V + +V IA D+
Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA-DIT 124

Query: 165 DGEALARTLTLIMVAMAAAPGFSPMLGGALSSWLGWQFTFAFVGVMAVVLAIHYNARLGE 224
DG+ AR + P+LGG + F + + + L E
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 225 THQADRRSAISIPAILKTYWQLLTDRRFIAPALTMSLVSGSLYAFFGMAPAILMVGFHFS 284
+H+ +RR ++ +A + + + G PA L V F
Sbjct: 184 SHKGERRPLRREALNPLASFRWARGMTVVAALMAVFF----IMQLVGQVPAALWVIFGED 239

Query: 285 PFGL---AIFFASTVFVVFGA---GLLVPRLAHRWGQARAVRVGLVIALTGSIVLLVGKE 338
F I + F + + ++ +A R G+ RA+ +G++ TG I+L
Sbjct: 240 RFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATR 299

Query: 339 DFVFFSAALMVFLLGMGM 356
++ F +++ G+GM
Sbjct: 300 GWMAFPIMVLLASGGIGM 317


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2201TCRTETB402e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 39.9 bits (93), Expect = 2e-05
Identities = 27/121 (22%), Positives = 55/121 (45%), Gaps = 1/121 (0%)

Query: 60 ASISFAMAIAQLVWGAAQPLFGAAADRWGPGRVIVIGAVMLAAGSALTTQVSSQWGLIFT 119
AS ++ L + ++G +D+ G R+++ G ++ GS + S + L+
Sbjct: 49 ASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIM 108

Query: 120 VGLLSAAGAGAGSFSILIGATAQRIPSERRSFAGGVINAGGSMGQFIFAPLNQAVMLAFG 179
+ AGA A ++++ A+ IP E R A G+I + +MG+ + + +
Sbjct: 109 ARFIQGAGA-AAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIH 167

Query: 180 W 180
W
Sbjct: 168 W 168


29Bpet2218Bpet2224Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2218-1153.377276cytoplasmic glycerophosphodiester
Bpet2219-1143.408018peptidyl-prolyl cis-trans isomerase D
Bpet22202153.945475acyl-CoA thioesterase I precursor
Bpet22211153.799482ABC transporter ATP-binding protein
Bpet22222173.836651isochorismatase family protein
Bpet22231152.893129AraC family transcriptional regulator
Bpet22242141.587731hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2222ISCHRISMTASE426e-07 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 41.9 bits (98), Expect = 6e-07
Identities = 39/198 (19%), Positives = 66/198 (33%), Gaps = 22/198 (11%)

Query: 7 RRALIVVDVQNEYFGGKLPIEYPDPQQSLANIGRAMDAAHAAGVPIVLV---QDIEPAES 63
R L++ D+Q YF + ANI + + G+P+V P +
Sbjct: 30 RAVLLIHDMQ-NYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPGSQNPDDR 88

Query: 64 PL----FARGSHGAELHESVVSR----PHDHHVVKGMPSAFAGTGLEAWLAERGIDTITV 115
L + G + E +++ D + K SAF T L + + G D + +
Sbjct: 89 ALLTDFWGPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLII 148

Query: 116 VGYMTHNCDDSTVKHAVHAGLRVEVLNDATGSVPYANSAGQASAEEIHRVLTVVMQSRFA 175
G H T A ++ + DA + E H++ R A
Sbjct: 149 TGIYAHIGCLVTACEAFMEDIKAFFVGDAVADF----------SLEKHQMALEYAAGRCA 198

Query: 176 AVMSTAQWIAGLNGAPMP 193
+ T + L AP
Sbjct: 199 FTVMTDSLLDQLQNAPAD 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2223RTXTOXINA330.001 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 33.4 bits (76), Expect = 0.001
Identities = 32/126 (25%), Positives = 55/126 (43%), Gaps = 10/126 (7%)

Query: 226 GGQAQYVEQPVPASVGADRLSGVLAWVSAHLDRAHDLDSLAARAAMSRRTFTRHFRQATG 285
G + Q + G D +SG+L+ +SA + + D+ A + T G
Sbjct: 226 GNKLQNLPNLDNIGAGLDTVSGILSAISASFILS-NADADTRTKAAAGVELTTKVLGNVG 284

Query: 286 GTVGQWLLSQRLALAQRLLETSDAPVETIAAQAGFG-TPLS---LRQHFRRALKVSPSAY 341
+ Q++++QR A L TS A IA+ +PLS + F+RA K+ Y
Sbjct: 285 KGISQYIIAQRAAQG---LSTSAAAAGLIASAVTLAISPLSFLSIADKFKRANKI--EEY 339

Query: 342 RREFRQ 347
+ F++
Sbjct: 340 SQRFKK 345


30Bpet2289Bpet2305Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2289-121-3.359018putative translational inhibitor
Bpet2290028-4.864815ATP-dependent DNA helicase RecG
Bpet2291142-9.166139LysR family transcriptional regulator
Bpet2292152-11.113146putative DNA-binding protein
Bpet2293157-12.398263hypothetical protein
Bpet2294257-13.273892short-chain sugar nucleotide oxidoreductase
Bpet2295360-15.048748sulfatase involved in polysaccharide
Bpet2296462-16.001161MPA2 family protein involved in capsular
Bpet2297557-15.430246outer membrane protein involved in
Bpet2298454-14.957342permease component of an ABC exporter involved
Bpet2299352-13.920615polysaccharide ABC transporter ATP-binding
Bpet2300249-12.222365hypothetical protein
Bpet2301037-8.447659hypothetical protein
Bpet2302-132-7.049983sugar nucleotide epimerase / oxidoreductase
Bpet2303037-6.343779sugar aminotransferase
Bpet2304-139-6.365432N-acylneuraminate cytidylyltransferase
Bpet2305-132-5.729935UDP-N-acetylglucosamine--N-acetylmuramyl-
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2290SECA310.026 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 30.6 bits (69), Expect = 0.026
Identities = 18/77 (23%), Positives = 32/77 (41%), Gaps = 5/77 (6%)

Query: 294 RLLQGDV-----GSGKTVVAAIAAAQAIACGAQVALMAPTEILAEQHFRKLVSWLQPLGV 348
L + + G GKT+ A + A G V ++ + LA++ + LG+
Sbjct: 93 VLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGL 152

Query: 349 NVAWLSGSLTAKARRQA 365
V + A A+R+A
Sbjct: 153 TVGINLPGMPAPAKREA 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2292HELNAPAPROT1432e-46 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 143 bits (361), Expect = 2e-46
Identities = 50/144 (34%), Positives = 76/144 (52%)

Query: 23 DRTAIAGELSKVLADSYTLYLMTHNFHWNVTGPLFNTLHQMFMTQYSEEWAALDDIAERI 82
++T + L+ L++ + LY H FHW V GP F TLH+ F Y +D IAER+
Sbjct: 9 NQTLVENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERL 68

Query: 83 RALGVHAPGTYREFSKLSSISEPGAVPDAMEMVRLLVKGNEAVSKTARAAFDKADSANDQ 142
A+G T +E+++ +SI++ G A EMV+ LV + +S ++ A+ D
Sbjct: 69 LAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYKQISSESKFVIGLAEENQDN 128

Query: 143 PTADLLTQRMDIHEKNAWMLRSLL 166
TADL ++ EK WML S L
Sbjct: 129 ATADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2294DHBDHDRGNASE747e-18 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 74.3 bits (182), Expect = 7e-18
Identities = 56/211 (26%), Positives = 85/211 (40%), Gaps = 13/211 (6%)

Query: 2 LITGATGSIGGALALEYAKAGVDTLILQGRRTERLAELAQLCRREGAQVETHALDVRDHA 61
ITGA IG A+A A G + E+L ++ + E E DVRD A
Sbjct: 12 FITGAAQGIGEAVARTLASQGAHIAAVD-YNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70

Query: 62 SLIAWLTQICEVHAPDLVIVNAGININVGSDRQGEIWQDVHELLDVNVKAAFATVHGVLP 121
++ +I P ++VN + G ++ VN F V
Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSD-EEWEATFSVNSTGVFNASRSVSK 129

Query: 122 FMRKRGQGQIALVSSLAAWRGLPETP--SYSASKAAIKVYGEAMRDGLAAEGIRFNVIMP 179
+M R G I V S A G+P T +Y++SKAA ++ + + LA IR N++ P
Sbjct: 130 YMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 180 GYVESPMCFDMPGPKPFLWTAARAAHAIRRG 210
G E+ M + LW A + +G
Sbjct: 188 GSTETDM-------QWSLWADENGAEQVIKG 211


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2296RTXTOXIND310.006 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.006
Identities = 21/175 (12%), Positives = 56/175 (32%), Gaps = 12/175 (6%)

Query: 150 AQQTLDIMLQESERFVNELSHRMAREQMNFAKSELANARRAYEERREALLTFQSANSLLD 209
Q I+ + E N+L ++ F R +E T+Q+
Sbjct: 149 EQTRYQILSRSIEL--NKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQ--KYQ 204

Query: 210 AEAAAKARAEVISELEASLTKERTTLKGLLATLDSNTPQVRQQ---RNRIQAMEQQLAAE 266
E + + A + + + + LD + + +Q ++ + E +
Sbjct: 205 KELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEA 264

Query: 267 TRRLVSQQGGDKLNVVASQYRNLTIDAAIAEEAYKFAVSSVETARIEASKKLRSL 321
L + +L + S+ + + + + +K + + + + + L
Sbjct: 265 VNELRVYKS--QLEQIESEILSAKEEYQLVTQLFK---NEILDKLRQTTDNIGLL 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2298ABC2TRNSPORT369e-05 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 36.1 bits (83), Expect = 9e-05
Identities = 48/197 (24%), Positives = 86/197 (43%), Gaps = 15/197 (7%)

Query: 37 LFEPIAHITFLMFLMTVVRGRHLPGFDYPIYLLTGLVPFFLMRNISLKMMEA----INAN 92
L EP+ ++ L + V+ GR + G Y +L G+V M + + + A +
Sbjct: 39 LAEPLIYLFGLGAGLGVMVGR-VGGVSYTAFLAAGMVATSAMTAATFETIYAAFGRMEGQ 97

Query: 93 RPLFA--YPNIKPFDTFLARLI---VECSLSACIYVLLLCAMGFWLGYDISIHAPLSWFV 147
R A Y ++ D L + + +L+ ++ A+G+ + P V
Sbjct: 98 RTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWLSLLYALP----V 153

Query: 148 ALLTGIAFAFGLGLVLCVVGEAMPNSKTFIRLMFLPLYLISGVIFPIWILPIRYMEWLLW 207
LTG+AFA LG+V+ + + + L+ P+ +SG +FP+ LPI + +
Sbjct: 154 IALTGLAFA-SLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQTAARF 212

Query: 208 NPYLHIIDNLRYSVFEH 224
P H ID +R + H
Sbjct: 213 LPLSHSIDLIRPIMLGH 229


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2299PF05272280.025 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.025
Identities = 12/20 (60%), Positives = 13/20 (65%)

Query: 37 LIGRNGAGKSTLMRLLGGLD 56
L G G GKSTL+ L GLD
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2302NUCEPIMERASE797e-19 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 79.4 bits (196), Expect = 7e-19
Identities = 40/195 (20%), Positives = 76/195 (38%), Gaps = 36/195 (18%)

Query: 6 TILITGGTGSFGNTFVPMTLAKY---NPKKVIIFSRDEMKQ-WDMARKFH-----DDPRV 56
L+TG G F+ ++K +V+ D + +D++ K P
Sbjct: 2 KYLVTGAAG-----FIGFHVSKRLLEAGHQVVGI--DNLNDYYDVSLKQARLELLAQPGF 54

Query: 57 RFFIGDVRDRERLYRALD--GVDYVVHAAATKIVPTAEYNPFECVKTNVDGAMNLIDACI 114
+F D+ DRE + + V + V + NP +N+ G +N+++ C
Sbjct: 55 QFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCR 114

Query: 115 DKGVKRVVALST---------------DKASSPINLYGATKLASDKLFVAGNAYSGEHGT 159
++ ++ S+ D P++LY ATK A++ + + YS +G
Sbjct: 115 HNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELM---AHTYSHLYGL 171

Query: 160 RFAVVRYGNVMGSRG 174
+R+ V G G
Sbjct: 172 PATGLRFFTVYGPWG 186


31Bpet2380Bpet2393Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet23800133.323601ABC transporter, ATP-binding protein
Bpet2381-1133.175351putative osmotically inducible protein Y
Bpet23820153.421018hypothetical protein
Bpet23830164.000805putative short chain dehydrogenase
Bpet23841183.907314hypothetical protein
Bpet23851173.249831glycosyltransferase
Bpet23860192.829553putative carboxyl-/carbamoyltransferase
Bpet23872203.657144glycosyltransferase
Bpet23882183.274171glycosyltransferase
Bpet23892173.172082glycosyltransferase
Bpet23903173.191972sugar nucleotide epimerase/dehydratase
Bpet23912173.808498hypothetical protein
Bpet23922133.260448glycosyltransferase
Bpet23932113.096366hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2380HTHFIS732e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 73.3 bits (180), Expect = 2e-15
Identities = 31/134 (23%), Positives = 58/134 (43%), Gaps = 9/134 (6%)

Query: 760 LSGVAIMVADDQEDARGLVAEVLADRGAAVHTCASGADVLAALRQASWPDLLVCDISLGD 819
++G I+VADD R ++ + L+ G V ++ A + + DL+V D+ + D
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAG-DGDLVVTDVVMPD 59

Query: 820 MEGYELIGRIRALEAERGAPLGERMPAVALSGHTGPEDRLRALLAGFQIHVAKPVDPREL 879
++L+ RI+ +P + +S ++A G ++ KP D EL
Sbjct: 60 ENAFDLLPRIKK--------ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTEL 111

Query: 880 LATVSAMLRPDTRR 893
+ + L RR
Sbjct: 112 IGIIGRALAEPKRR 125


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2383DHBDHDRGNASE842e-21 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 83.6 bits (206), Expect = 2e-21
Identities = 46/187 (24%), Positives = 74/187 (39%), Gaps = 2/187 (1%)

Query: 7 LAGRVVLVTGGGSGLGAAICDMLAAEGASVVVADLDETRAHDCARRLSERGAAACATVMD 66
+ G++ +TG G+G A+ LA++GA + D + + L A A D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 67 VGDPEQVAAALDLAMQRYGKLDAVVNNAGIDVTASIDELDVAAWERVLRTNLTGPFLVAK 126
V D + + G +D +VN AG+ I L WE N TG F ++
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 127 LARNRLTPH--GHIVNIASTAARRAWPNASAYHASKWGLLGLSHALHAELRGMGLKVSAV 184
+ G IV + S A + +AY +SK + + L EL ++ + V
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 185 IAGGMRT 191
G T
Sbjct: 186 SPGSTET 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2390NUCEPIMERASE1761e-54 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 176 bits (447), Expect = 1e-54
Identities = 79/335 (23%), Positives = 129/335 (38%), Gaps = 35/335 (10%)

Query: 6 RALVAGGAGFLGAHLCRRLLLQGWEVICVDNFHTGRSENL----AGLAAHPGLTVIRQDI 61
+ LV G AGF+G H+ +RLL G +V+ +DN + +L L A PG + D+
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 62 A-LPLPAEL----HIDCIYNLACPASPVHYQ-ADPVATLQTCVQGATQLLELAARTG-AR 114
A +L H + ++ + V Y +P A + + G +LE
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLA-VRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 115 ILQASTSEVYGDPLEHPQREGYWGHVNPVGPRSCYDEGKRCAETLFMEYGRRRGVVVKIA 174
+L AS+S VYG + P + P S Y K+ E + Y G+
Sbjct: 121 LLYASSSSVYGLNRKMPFST----DDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGL 176

Query: 175 RIFNTYGPGMAADDGRVVSNFIVQALAGHPLTVYGDGSQTRSFCYVDDLVDGLLRLMNSP 234
R F YGP D + F L G + VY G R F Y+DD+ + ++RL +
Sbjct: 177 RFFTVYGPWGRPD--MALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVI 234

Query: 235 DQFSQPV-----------------NLGNPAEISVLRMAELVRELTGSRAPLQFRDLPRDD 277
N+GN + + ++ + + + G A L D
Sbjct: 235 PHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGD 294

Query: 278 PTHRCPDITLAREQLRWRPTTPLSAGLARTVDYFR 312
D E + + P T + G+ V+++R
Sbjct: 295 VLETSADTKALYEVIGFTPETTVKDGVKNFVNWYR 329


32Bpet2499Bpet2507Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2499-1123.035433hypothetical protein
Bpet25000122.722666hypothetical protein
Bpet25010132.774787hypothetical protein
Bpet2502-1122.989459putative deoxyribonuclease
Bpet25030114.276312DNA polymerase III, delta' subunit
Bpet2504-2103.626516thymidylate kinase
Bpet2505093.434761hypothetical protein
Bpet25060103.506813hypothetical protein
Bpet2507-1133.345904hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2501IGASERPTASE300.017 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 29.6 bits (66), Expect = 0.017
Identities = 12/61 (19%), Positives = 26/61 (42%), Gaps = 2/61 (3%)

Query: 210 KAGKLATELGTLIAKAERRR-DAQRGGQAPATAAPAAPAPA-PAATRPQAEPAPPSAQTE 267
+ T+ + K E+ + + ++ + P + +P +PQAEPA + T
Sbjct: 1094 ETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTV 1153

Query: 268 S 268
+
Sbjct: 1154 N 1154


33Bpet2571Bpet2576Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet25711113.603941putative monovalent cation/H+ antiporter subunit
Bpet25722114.066800putative monovalent cation/H+ antiporter subunit
Bpet25731104.130663putative monovalent cation/H+ antiporter subunit
Bpet25741113.798564putative monovalent cation/H+ antiporter subunit
Bpet25750114.009901putative secreted protein
Bpet25760104.204533putative GTP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2576CHANLCOLICIN412e-05 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 40.8 bits (95), Expect = 2e-05
Identities = 58/246 (23%), Positives = 90/246 (36%), Gaps = 33/246 (13%)

Query: 482 AALHTAQAERQGLLARLGAVSLAEAETRAAAHERARRDLDMARQHLRIQAPEGVDALRAA 541
AA+H L + A A A+ A A +A+ + D Q L+ E ALR
Sbjct: 49 AAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNE---ALRHN 105

Query: 542 QRQAHERRAQLQALRAGLPDAAAPVSLEQAQQALRAATAEAGHAAQEVVSARTALDTQQA 601
+ A A + + L +A++ R A A QE R ++ ++A
Sbjct: 106 ASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKA 165

Query: 602 STQVLQAQWTARQGEFDAAGRAAQREQRGARLVEARARRDTLAQRAQAAQAALQAHQPEI 661
T+ A E+R A L E A+ + AQ L A Q E+
Sbjct: 166 ETE-------------RQLKLAEAEEKRLAALSEE-------AKAVEIAQKKLSAAQSEV 205

Query: 662 IEQDARRFEQSAALARDAHQRRHAELLQLQGKLEQAQAQGLGEQLLQAQADAQRLARRRD 721
++ D ++ L+ H R AE+ L GK +L QA A + L
Sbjct: 206 VKMDGEIKTLNSRLSSSIHARD-AEMKTLAGKR---------NELAQASAKYKELDELVK 255

Query: 722 EFAMRA 727
+ + RA
Sbjct: 256 KLSPRA 261


34Bpet2585Bpet2612Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2585-1173.457244put. ABC transport protein, inner membrane
Bpet2586-1164.804595sugar ABC transporter ATP-binding protein
Bpet2587-2184.733191ABC transporter ATP-binding protein
Bpet2588-1183.602158hypothetical protein
Bpet25890154.638541hypothetical protein
Bpet25901144.353378TetR family transcriptional regulator
Bpet25911143.937586HlyD family secretion protein
Bpet25920143.100304putative ATP-binding component of a transport
Bpet25931134.024424ABC-type multidrug transport system, permease
Bpet25941114.605066outer membrane exporter protein
Bpet25952113.181227hypothetical protein
Bpet25962123.249363Ser/Thr-rich protein T10
Bpet25973112.499737hypothetical protein
Bpet25981112.253762hypothetical protein
Bpet2599-1111.130782hypothetical protein
Bpet26000121.772387hypothetical protein
Bpet26010162.914396putative 6-pyruvoyl tetrahydrobiopterin
Bpet26020152.666005putative periplasmic solute-binding protein
Bpet26030152.890869class II aldolase/adducin domain protein
Bpet26041164.621763transcriptional regulator
Bpet26052145.150852MarR family transcriptional regulator
Bpet26064135.157225hypothetical protein
Bpet26074126.062163hypothetical protein
Bpet26082135.211402HlyD family secretion protein
Bpet26093135.414970outer membrane efflux protein
Bpet26102144.724672esterase YpfH
Bpet26111134.014443hypothetical protein
Bpet26120133.721725hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2590HTHTETR692e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 68.9 bits (168), Expect = 2e-16
Identities = 28/180 (15%), Positives = 73/180 (40%), Gaps = 15/180 (8%)

Query: 12 LGRPARPQRADSRDAMLDVATALFAAQGVAATTIAHIARRADVTPAMVHYYFKNREQLID 71
+ R + + ++R +LDVA LF+ QGV++T++ IA+ A VT ++++FK++ L
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 72 VVVAERLAPVIASVWAPAALPAGNGPGAAPPTPPEPRAMVAQVVARIMQCAAERP----W 127
+ + + P +P +++ +++ +++
Sbjct: 61 EIWELSESNIGELE-----------LEYQAKFPGDPLSVLREILIHVLESTVTEERRRLL 109

Query: 128 LAPLWMREVVNEGGQLREKVFRYLPVERLHAFAATITSAQQQGAVNPGIEPRLVFLSILG 187
+ ++ + + ++ R L +E T+ + + + R + + G
Sbjct: 110 MEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRG 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2591RTXTOXIND506e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 50.2 bits (120), Expect = 6e-09
Identities = 37/280 (13%), Positives = 82/280 (29%), Gaps = 25/280 (8%)

Query: 64 ARGQQVQAGAPLFALEADPEAQAQREARARLASAQAQRQDLATGKRAPEVDVVRAQLAQA 123
++V L + + + L +A+R + E +
Sbjct: 179 VSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLD 238

Query: 124 EAEAKRAAAQLARDRVQFQAGGIARAQLDDSRAQAQSSAARVRELRAQLQVAGLPGR--- 180
+ + +A+ V Q A + ++Q L A+ + +
Sbjct: 239 DFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKN 298

Query: 181 --DEQLRAQDAQVEAARAGLAQADWALAQKQVAAAQAARVFD-TLYRVGEWVPAGSPVVR 237
++LR + LA+ + + A + +V ++ G V ++
Sbjct: 299 EILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMV 358

Query: 238 LLPPGN-IKLRFFVPETALGGLRSGQAVRARCDACGE----PVAATISYIAAEAEYTPPV 292
++P + +++ V +G + GQ + +A + + I +A
Sbjct: 359 IVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA------ 412

Query: 293 IYSRDSRGKLVYMV------EAHPAPRDATRLHPGQPVEV 326
D R LV+ V L G V
Sbjct: 413 --IEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTA 450


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2592BACINVASINB290.027 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 29.0 bits (64), Expect = 0.027
Identities = 12/38 (31%), Positives = 24/38 (63%)

Query: 128 QAVEQALEGLGLQSRANQLTGSLSGGWKQRLALAACLL 165
+A+ +ALEGLG+ + ++ GS+ G +A+ A ++
Sbjct: 387 KAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMVAVIV 424


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2593ABC2TRNSPORT512e-09 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 50.7 bits (121), Expect = 2e-09
Identities = 42/173 (24%), Positives = 75/173 (43%), Gaps = 2/173 (1%)

Query: 208 AMTRERERGTMENLLATPVRPLEVMTGKIVPYIAIGLIQATIILLAALYVFHVPLMGSLL 267
A R + T E +L T +R +++ G++ + I + A + + + SLL
Sbjct: 90 AFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWL-SLL 148

Query: 268 AVYLAALLFVAANLTVGITLSSLAQNQLQAMQLTMFYFLPNILLSGFMFPFQGMPVWAQH 327
L A ++G+ +++LA + + P + LSG +FP +P+ Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 328 IGNLLPLTYFNRLIRGILLKGNGWADLWPHVWPLLLFTALIMALAVKFYRRTL 380
LPL++ LIR I+L D+ HV L ++ + L+ RR L
Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPV-VDVCQHVGALCIYIVIPFFLSTALLRRRL 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2594RTXTOXIND310.012 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.0 bits (70), Expect = 0.012
Identities = 21/184 (11%), Positives = 49/184 (26%), Gaps = 27/184 (14%)

Query: 302 PLGVPSQLTRQRPDILAAEALWHRAAADVGVATANLYPRFTLTGSFGSQRTRAGDVADGV 361
LG + + + +L A R N P L Q +V
Sbjct: 129 ALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVL--- 185

Query: 362 NVWSLALGLTQPLFHGGELRARRRAAEAAYQAAAAAYRDTVLQGLQQVADALSAVQADAD 421
L + + + +Q L + V A +
Sbjct: 186 -----------------RLTSLIKEQFSTWQNQKYQKE----LNLDKKRAERLTVLARIN 224

Query: 422 TLQARAEAERQAEAAYRITAQQYQAGGVSQLALLDAQREQLRTRAERIQAQADRHADTAA 481
+ + E+ + + +++ A+L+ + + + E ++ +
Sbjct: 225 RYENLSRVEKSRLDDFSSLLHK---QAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESE 281

Query: 482 LLQA 485
+L A
Sbjct: 282 ILSA 285



Score = 30.2 bits (68), Expect = 0.024
Identities = 16/116 (13%), Positives = 38/116 (32%), Gaps = 6/116 (5%)

Query: 372 QPLFHGGELRAR--RRAAEAAY-QAAAAAYRDTVLQGLQQVADALSAVQADADTLQARAE 428
L L A +++ QA R +L ++ D Q +E
Sbjct: 122 DVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSE 181

Query: 429 AERQAEAAYRITAQQYQAGGVSQLALLDAQREQLRTRAERIQAQADRHADTAALLQ 484
E + +Q+ +Q + ++ R + A+ +R+ + + + +
Sbjct: 182 EEVLRLTSLI--KEQFSTWQ-NQKYQKELNLDKKRAERLTVLARINRYENLSRVEK 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2608RTXTOXIND565e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 56.0 bits (135), Expect = 5e-11
Identities = 34/207 (16%), Positives = 68/207 (32%), Gaps = 17/207 (8%)

Query: 88 QLAYDQAQAAVRARQVARDQAARDARRNRSLGKLVSAEALEQSQARLQQAEAALAEAEVQ 147
+ Y +A +R + +Q + + +LV+ + +L+Q + ++
Sbjct: 258 ENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLE 317

Query: 148 RATARLNLARSRVVAPTDGRVTNLDLR-VGSYATAAHAVMALV-DASSFYVEGYFEETKL 205
A S + AP +V L + G T A +M +V + + V + +
Sbjct: 318 LAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDI 377

Query: 206 AQIHEGDAVSVTLMGDSRQIHGHVQSIALGI-ADR--DRGTGANLLPNVNPTFNWVRLAQ 262
I+ G + + +G++ I D D+ G V
Sbjct: 378 GFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGL------------VFNVI 425

Query: 263 RIPVRVQIDEVPKGVRLVAGQTATVEI 289
+ K + L +G T EI
Sbjct: 426 ISIEENCLSTGNKNIPLSSGMAVTAEI 452



Score = 55.2 bits (133), Expect = 9e-11
Identities = 28/172 (16%), Positives = 60/172 (34%), Gaps = 20/172 (11%)

Query: 4 LNTLRRPVVGKFFVTALTV--CAAVYAGWQLWTHYEVE-----PWTRDGRVKAYVVQVAP 56
L + PV + + A + + + E+ T GR K + P
Sbjct: 46 LELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKE----IKP 101

Query: 57 DVSGLVTAVPVHDNQDVKAGDVLFEIDRARFQLAYDQAQAAVRARQV--ARDQAARDARR 114
+ +V + V + + V+ GDVL ++ + + Q+++ ++ R Q +
Sbjct: 102 IENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIE 161

Query: 115 NRSLGKLV-------SAEALEQSQARLQQAEAALAEAEVQRATARLNLARSR 159
L +L + E+ + + + Q+ LNL + R
Sbjct: 162 LNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKR 213


35Bpet2627Bpet2644Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet26272181.432898hypothetical protein
Bpet26282171.076366hypothetical protein
Bpet26291161.588987putative mannose-6-phosphate isomerase
Bpet26301151.641352hypothetical protein
Bpet26313141.880386hypothetical protein
Bpet26321111.300342hypothetical protein
Bpet26330130.486592glyoxalase family protein
Bpet26341110.0831552-oxo-hept-3-ene-1,7-dioate hydratase
Bpet26350111.5602102,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase
Bpet26360120.841559hypothetical protein
Bpet26370131.003157alcohol dehydrogenase, zinc-containing
Bpet26382151.000555*MerR family transcriptional regulator
Bpet26390123.657857integration host factor subunit alpha
Bpet26400123.597427phenylalanyl-tRNA synthetase subunit beta
Bpet26411122.886712phenylalanyl-tRNA synthetase subunit alpha
Bpet26421143.65355250S ribosomal protein L20
Bpet26430144.14759550S ribosomal protein L35
Bpet2644-1154.607223putative chelatase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2635PHPHTRNFRASE491e-08 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 48.6 bits (116), Expect = 1e-08
Identities = 41/197 (20%), Positives = 69/197 (35%), Gaps = 34/197 (17%)

Query: 81 QIKQILDAGAQT---LLVPMIQSAEEAAAAVSAMRYPPHGVRGLGSALARASRWNRIPDY 137
Q++ +L A ++ PMI + EE A + M+ + G ++ +
Sbjct: 374 QLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDVSDSIEVG----- 428

Query: 138 AQRANDEMCLLVQIETPRGLQALDEILALEGVDGVFIGPADL----------SASMGYLS 187
+ +E P A + + VD IG DL + + YL
Sbjct: 429 -----------IMVEIPSTAVAANLFA--KEVDFFSIGTNDLIQYTMAADRMNERVSYLY 475

Query: 188 NPDHPDVCAAIDDAIVRIARAGKAAGI---LHGDPAQARHYLDLGATFVAVGVDATLLAR 244
P HP + +D I GK G+ + GD L LG ++ + L AR
Sbjct: 476 QPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPAR 535

Query: 245 AAEKLAQSFKDQPAAQA 261
+ + +P AQ
Sbjct: 536 SQLLKLSKEELKPFAQK 552


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2639DNABINDINGHU1181e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 118 bits (298), Expect = 1e-38
Identities = 37/89 (41%), Positives = 52/89 (58%)

Query: 9 TKAELAELLFERVGLNKREAKDIVDTFFEEIRDALARGDSVKLSGFGNFQVRNKPPRPGR 68
K +L + E L K+++ VD F + LA+G+ V+L GFGNF+VR + R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 69 NPKTGETIPIAARRVVTFHASQKLKSVVE 97
NP+TGE I I A +V F A + LK V+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAVK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2641adhesinb300.011 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 30.2 bits (68), Expect = 0.011
Identities = 8/38 (21%), Positives = 16/38 (42%)

Query: 44 KGLAKLDPDQKRELGARINQAKQRIEALLNERRAQLAQ 81
K L++ DP K + +++ AL E + +
Sbjct: 157 KRLSEKDPANKETYEKNLKAYVEKLSALDKEAKEKFNN 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2644HTHFIS310.009 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.3 bits (71), Expect = 0.009
Identities = 48/245 (19%), Positives = 77/245 (31%), Gaps = 36/245 (14%)

Query: 127 PVGAPLALALAVAREQPDATLVLPADSATVAAWVPGLQVLAAGA---------LAEVAAH 177
P L + + +PD +++ + T + + GA L E+
Sbjct: 58 PDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASE---KGAYDYLPKPFDLTELIGI 114

Query: 178 LSGAAPLPRAEPGAWPAAAASPCLSDVRGQPM--ARRALEVAAAGAHSLLMVGPPGAGKS 235
+ A P+ P + R M R L +L++ G G GK
Sbjct: 115 IGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKE 174

Query: 236 MLAQRLPGLLPPLSRTQALEAAALAGLAGPAGMAAALQGQPPFRAPHHGASAAALVGGGA 295
++A+ L R A +A + + + L G H A G
Sbjct: 175 LVARALHDYGK--RRNGPFVAINMAAIPRDL-IESELFG--------HEKGAFT---GAQ 220

Query: 296 RPRPGEATLAHHGVLFLDELPEFDRRALEALREPLETG---RVAIARARHSVQYPARFQL 352
G A G LFLDE+ + A L L+ G V + ++
Sbjct: 221 TRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPI-----RSDVRI 275

Query: 353 VAAMN 357
VAA N
Sbjct: 276 VAATN 280


36Bpet2660Bpet2672Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2660-193.225255penicillin-binding protein
Bpet2661-192.564686hypothetical protein
Bpet26620120.371137ATP-dependent Clp protease adaptor protein ClpS
Bpet26631112.144685cold-shock protein
Bpet26641112.820250hypothetical protein
Bpet2665-1103.682721beta-ketoadipyl CoA thiolase
Bpet2666093.859860putative chloride channel protein-related
Bpet2667-3124.463796hypothetical protein
Bpet2668-3135.120656exodeoxyribonuclease VII large subunit
Bpet26690203.916415biopolymer transport protein
Bpet2670-2194.506676tetraacyldisaccharide 4'-kinase
Bpet2671-1183.535439hypothetical protein
Bpet2672-1183.1327213-deoxy-manno-octulosonate cytidylyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2661IGASERPTASE320.040 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 31.6 bits (71), Expect = 0.040
Identities = 31/187 (16%), Positives = 54/187 (28%), Gaps = 15/187 (8%)

Query: 30 APSAPDTAAAAVQPSAQSDHTTQSRPQRPASAQPAAAT-VGQADPFAVLDCQAREYNDTL 88
A PS S++ +R PA AT + A Q + +
Sbjct: 995 TNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKN 1054

Query: 89 ALAVTFTQPVDRKAGLDSFLNVV-DTGAVKASEDGQDAAAA------SKASVVAAGAAAA 141
T T +R+ ++ NV +T + ++ G + A+V A
Sbjct: 1055 EQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKV 1114

Query: 142 QGRPVQGAWVVGDN------PRMVYFPYVQPQRSYAVRLRAALPGAQAGATLGADLQCSV 195
+ Q V P +P R + P +Q T Q +
Sbjct: 1115 ETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTT-ADTEQPAK 1173

Query: 196 QTPAMPP 202
+T +
Sbjct: 1174 ETSSNVE 1180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2669FLAGELLIN280.025 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 28.1 bits (62), Expect = 0.025
Identities = 8/41 (19%), Positives = 18/41 (43%), Gaps = 2/41 (4%)

Query: 158 AFGILIAIPAMIAHRYLRGRVDGLLNAMEQIAA--RVARAS 196
A I +++ L L +A+E++++ R+ A
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAK 41


37Bpet2756Bpet2761Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet27560113.726645hypothetical protein
Bpet27571123.839012phosphoribosylformylglycinamidine synthase
Bpet27582154.394160transcription elongation factor GreB
Bpet27593153.960572hypothetical protein
Bpet27601124.251505hydrolase
Bpet2761-1103.696546DNA internalization-related competence protein
38Bpet2834Bpet2839Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2834-216-3.274111threonyl-tRNA synthetase
Bpet2835-225-3.536901two-component response regulator
Bpet2836-228-4.509022hypothetical protein
Bpet2837-228-4.087258LysR family transcriptional regulator
Bpet2838-320-3.815699putative short chain dehydrogenase
Bpet2839-323-4.364226membrane protein resembling polysaccharide
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2835HTHFIS676e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.2 bits (164), Expect = 6e-15
Identities = 27/121 (22%), Positives = 49/121 (40%), Gaps = 4/121 (3%)

Query: 45 DRLRIAILDDHPVITLGVAAYLRSQPDFDIVHAETTSEALVRSLKHQPCDVAVVDFYLPR 104
I + DD I + L S+ +D+ + + D+ V D +P
Sbjct: 2 TGATILVADDDAAIRTVLNQAL-SRAGYDVRITSNAATLWRW-IAAGDGDLVVTDVVMPD 59

Query: 105 QPWDGMDFIRRLRRQHPHMAIITFSAGAPAETEYAAFRAGAHGYLPKSASMPVLVEVIRA 164
+ + D + R+++ P + ++ SA T A GA+ YLPK + L+ +I
Sbjct: 60 E--NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117

Query: 165 A 165
A
Sbjct: 118 A 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2838DHBDHDRGNASE682e-15 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 67.8 bits (165), Expect = 2e-15
Identities = 55/196 (28%), Positives = 91/196 (46%), Gaps = 6/196 (3%)

Query: 1 MADHSINGKVALIAGGAKNLGGLIARDLAGQGARAVVIHYNSASSREAAEATVAAVQTSG 60
M I GK+A I G A+ +G +AR LA QGA + YN E E V++++
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNP----EKLEKVVSSLKAEA 56

Query: 61 AQGVALQGDLTTAGAVEKLFADAIAAVGRPDIAINTVGKVLKKPFTEITEAEYDEMAAIN 120
A D+ + A++++ A +G DI +N G + +++ E++ ++N
Sbjct: 57 RHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVN 116

Query: 121 SKAAFFFLKEAGRHVND--NGKIVTLVTSLLGAFTPFYAAYAGSKAPVEHYTRAAAKEFG 178
S F + +++ D +G IVT+ ++ G AAYA SKA +T+ E
Sbjct: 117 STGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 179 ARGISVNAVGPGPMDT 194
I N V PG +T
Sbjct: 177 EYNIRCNIVSPGSTET 192


39Bpet2854Bpet2862Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet28540174.004643LysR family transcriptional regulator
Bpet28552164.537758putative secreted protein
Bpet28562154.716167general secretion pathway protein G
Bpet28571155.884963general secretion pathway protein H
Bpet28582156.491578general secretion pathway protein I
Bpet28592145.712154general secretion pathway protein J
Bpet28600134.307568general secretion pathway protein K
Bpet28610154.092589hypothetical protein
Bpet2862-1153.228795general secretion pathway protein M
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2856BCTERIALGSPG1688e-57 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 168 bits (427), Expect = 8e-57
Identities = 65/142 (45%), Positives = 89/142 (62%), Gaps = 8/142 (5%)

Query: 17 RPRARQQGFTLIEIMVVIVIMGILAALVVPRVLDRPDQARRVAARQDISGLMQALKLYRL 76
R +Q+GFTL+EIMVVIVI+G+LA+LVVP ++ ++A + A DI L AL +Y+L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKL 61

Query: 77 DNGRYPNAAQGLQALVRRP---DGARNWRP--YLDRLPDDPWGHPYQYLNPGVKGEIDVF 131
DN YP QGL++LV P A N+ Y+ RLP DPWG+ Y +NPG G D+
Sbjct: 62 DNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLL 121

Query: 132 TFGPDNKAGGEEDDADIGSWDL 153
+ GPD + G E+ DI +W L
Sbjct: 122 SAGPDGEMGTED---DITNWGL 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2857BCTERIALGSPH525e-11 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 51.9 bits (124), Expect = 5e-11
Identities = 17/90 (18%), Positives = 32/90 (35%)

Query: 8 ISERGFTLIEMLVVVAIIAIAASMVGLSVTSSSGRALRADAERLVDAFAVAQSEARSDGR 67
+ +RGFTL+EM++++ ++ ++A MV L+ +S + R Q G+
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQ 60

Query: 68 AILWRADERGWSFERRGRPARVSAQDDGPQ 97
W F
Sbjct: 61 FFGVSVHPDRWQFLVLEARDGADPAPADDG 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2858BCTERIALGSPG412e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 40.6 bits (95), Expect = 2e-07
Identities = 20/64 (31%), Positives = 39/64 (60%), Gaps = 3/64 (4%)

Query: 1 MPSSRQQRGFTLIEVLVALAIISVAMGAAMRATQVMLDNSRAIRDKTLALLAA-DNTLAR 59
M ++ +QRGFTL+E++V + II V A++ +M + +A + K ++ + A +N L
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVL--ASLVVPNLMGNKEKADKQKAVSDIVALENALDM 58

Query: 60 LRLE 63
+L+
Sbjct: 59 YKLD 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2859BCTERIALGSPG290.008 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 28.7 bits (64), Expect = 0.008
Identities = 9/27 (33%), Positives = 19/27 (70%)

Query: 5 RRCAPQQGFTLIEVLIAIALMALVSLL 31
R Q+GFTL+E+++ I ++ +++ L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASL 28


40Bpet2878Bpet2898Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2878213-0.002903citrate synthase
Bpet2879119-1.804611acyl-CoA transferase/carnitine dehydrastase
Bpet2880325-2.860447LysR family transcriptional regulator
Bpet2881434-4.228313hypothetical protein
Bpet2882434-4.818686hypothetical protein
Bpet2883227-4.249758hypothetical protein
Bpet2884123-3.633826hypothetical protein
Bpet2885120-3.179476hypothetical protein
Bpet2886016-2.326974hypothetical protein
Bpet2887012-1.461327hypothetical protein
Bpet2888112-1.072794putative bacteriophage protein
Bpet28891150.806408putative exodeoxyribonuclease III
Bpet28901142.674208*hypothetical protein
Bpet28911142.943385hypothetical protein
Bpet28920153.671016putative thiosulfate sulfurtransferase
Bpet28931144.038554manganese transport protein
Bpet28942154.029772putative transcriptional regulator
Bpet28953153.967756MFS efflux transporter
Bpet28962153.689586hypothetical protein
Bpet28972162.847459pyridoxal kinase
Bpet28982183.246095hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2883PYOCINKILLER280.009 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 28.2 bits (62), Expect = 0.009
Identities = 24/80 (30%), Positives = 38/80 (47%), Gaps = 3/80 (3%)

Query: 42 RAWHQANADQLAAKRATAEAVRQAERAAR-LEAFTDATIRAVAAMLEDGHTLDEACKSLI 100
+A +A A A ++A AEA R+AE AR A A A+ A T A + LI
Sbjct: 211 KASIEAAAANKAREQAAAEAKRKAEEQARQQAAIRAANTYAMPANGSVVAT--AAGRGLI 268

Query: 101 GNAFRFGACHETLNELVAIL 120
A + + +++ +A+L
Sbjct: 269 QVAQGAASLAQAISDAIAVL 288


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2895TCRTETB448e-07 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 43.7 bits (103), Expect = 8e-07
Identities = 27/140 (19%), Positives = 59/140 (42%), Gaps = 1/140 (0%)

Query: 41 DAFIVAAFLPLMAADLGVTPSVAGHSVTAFAVAYALLAPVIATLTARVPRRTLLCVSLAL 100
+ ++ LP +A D P+ TAF + +++ V L+ ++ + LL + +
Sbjct: 29 NEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIII 88

Query: 101 LGAANIGSALATSM-PWLIASRIAAAATAAAYTPNAGAVAAALVRTDFRARALAIVIGGL 159
++ + S LI +R A AAA+ V A + + R +A ++ +
Sbjct: 89 NCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIV 148

Query: 160 TVATALGVPLGRVASTMLSW 179
+ +G +G + + + W
Sbjct: 149 AMGEGVGPAIGGMIAHYIHW 168



Score = 31.4 bits (71), Expect = 0.006
Identities = 20/103 (19%), Positives = 39/103 (37%), Gaps = 2/103 (1%)

Query: 283 YLSGVGTDRRGARRVLLTAYLIMAVALGGLAWLAASAEPMLPITAALVGLWGASSWAQSP 342
Y+ G+ DRRG VL ++V+ ++L + +T +V + G S+ ++
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETT--SWFMTIIIVFVLGGLSFTKTV 368

Query: 343 AQQHRLIASAPQHGALVVALNASAIYFGIALGTAIGARLVETG 385
+ Q ++L + G AI L+
Sbjct: 369 ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIP 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2896ALARACEMASE373e-05 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 37.4 bits (87), Expect = 3e-05
Identities = 24/135 (17%), Positives = 49/135 (36%), Gaps = 13/135 (9%)

Query: 112 ALDSLRVAEALDRRLQAEGRALDVFVQVNTSNEASKFGLPPEQAAAFVRELPAYSSLRVR 171
+ S +AL LD++++VN+ ++ G P++ ++L A +++
Sbjct: 99 CVHSNWQLKALQNARL--KAPLDIYLKVNSG--MNRLGFQPDRVLTVWQQLRAMANVGEM 154

Query: 172 GLMTLALFSPDPALVRPCFVRLRELRDRLRQEAPAGIAIDELSMGMSGDYALAIEEGATT 231
LM+ + P + R+ + + L S+ S E
Sbjct: 155 TLMSHFAEAEHPDGISGAMARIEQAAEGLEC---------RRSLSNSAATLWHPEAHFDW 205

Query: 232 VRVGQAIFGARALPD 246
VR G ++GA
Sbjct: 206 VRPGIILYGASPSGQ 220


41Bpet2907Bpet2983Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2907-1203.682344periplasmic solute-binding protein
Bpet29080204.705730putative GMC oxidoreductase
Bpet2909-1184.696476putative secreted protein
Bpet2910-2163.620290dihydroxy-acid dehydratase
Bpet2911-1184.681616LysR family transcriptional regulator
Bpet2912-1184.7648273-hydroxybutyryl-CoA dehydrogenase
Bpet2913-1184.334447hypothetical protein
Bpet29140204.035540hypothetical protein
Bpet29150193.699810phosphoenolpyruvate carboxykinase
Bpet29165165.415102multidrug resistance protein norM
Bpet29173144.420508allantoate amidohydrolase
Bpet29180153.173694hypothetical protein
Bpet29191153.221945hypothetical protein
Bpet29201152.692630GNAT family acetyltransferase
Bpet29210172.823080GntR family transcriptional regulator
Bpet2922-1171.485967hypothetical protein
Bpet2923-2192.822692glutathione reductase
Bpet29240173.679065hypothetical protein
Bpet2925-1153.581352transposase for IS1663
Bpet29260184.173812phospho-2-dehydro-3-deoxyheptonate aldolase
Bpet29271154.584786isoquinoline 1-oxidoreductase, alpha subunit
Bpet29280154.438170putative oxidoreductase subunit
Bpet29292143.799225AraC family transcriptional regulator
Bpet29302143.641944putative lipoprotein
Bpet29313154.285956hypothetical protein
Bpet29322133.873123hypothetical protein
Bpet29333143.691016hypothetical protein
Bpet29344123.988249PhnB protein
Bpet29351123.366578putative transmembrane efflux protein
Bpet29360123.131384hypothetical protein
Bpet2937-1122.665480sulfate permease family protein
Bpet2938-1101.631237hypothetical protein
Bpet29390102.741695acetyltransferase
Bpet2940282.332243hypothetical protein
Bpet2941-1132.514161hypothetical protein
Bpet2942-2133.279484hypothetical protein
Bpet29430124.053868hypothetical protein
Bpet29441114.870207putative RNA polymerase sigma factor
Bpet29450124.764720hypothetical protein
Bpet29463125.576961putative outer membrane proton channel
Bpet29471144.218614OmpA-family protein
Bpet29483153.655557hypothetical protein
Bpet29492163.214398TetR family transcriptional regulator
Bpet29501172.389926AraC family transcriptional regulator
Bpet29512142.817078hypothetical protein
Bpet29521171.924539putative ABC-transporter membrane-spanning
Bpet29534164.421103hypothetical protein
Bpet29544164.169702GNAT family acetyltransferase
Bpet29552124.116107galactarate dehydratase
Bpet29562134.510798putative MFS permease
Bpet29570124.365574hypothetical protein
Bpet29580134.501042hypothetical protein
Bpet2959-1203.340502hypothetical protein
Bpet2960-1213.725420N-carbamyl-L-amino acid amidohydrolase
Bpet2961-2213.182536LysR family transcriptional regulator
Bpet2962-3232.992849putative fatty oxidation complex alpha subunit
Bpet2963-3222.0798143-ketoacyl-CoA thiolase
Bpet2964-3201.234209putative acyl-CoA dehydrogenase
Bpet2965-2142.201437putative acyl-CoA dehydrogenase
Bpet2966-1131.923956hypothetical protein
Bpet29671132.533577putative secreted protein
Bpet29681143.148102putative secreted protein
Bpet29692153.441090putative secreted protein
Bpet29704174.397026AraC family transcriptional regulator
Bpet29712172.708189DMT family permease
Bpet29722162.719520MFS family transporter
Bpet2973-1122.284805MarR family transcriptional regulator
Bpet29740101.699195putative DNA polymerase bacteriophage-type
Bpet29751111.021564hypothetical protein
Bpet29761121.211612glutathione S-transferase family protein
Bpet29773122.075215hypothetical protein
Bpet29780171.948379MarR family transcriptional regulator
Bpet29791151.754309putative glutathione S-transferase
Bpet29801152.593717putative SugE protein
Bpet29811162.429821hypothetical protein
Bpet29820163.118589ArsR family transcriptional regulator
Bpet29830193.104769hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2926PF07520300.019 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 29.9 bits (67), Expect = 0.019
Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 6/41 (14%)

Query: 299 QVDVARDIAAQLAQGESRIIGVMIESHLEEGRQDLKPGVPL 339
+VD+ DI G SR G++IE E R DL PL
Sbjct: 278 EVDLVLDI------GNSRTCGILIERFPGETRVDLTRSFPL 312


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2933FIMREGULATRY280.025 Escherichia coli: P pili regulatory PapB protein si...
		>FIMREGULATRY#Escherichia coli: P pili regulatory PapB protein

signature.
Length = 104

Score = 27.6 bits (61), Expect = 0.025
Identities = 9/18 (50%), Positives = 13/18 (72%)

Query: 42 DVLVDGHNRYELCRKHGI 59
D LV GH+R E+C K+ +
Sbjct: 53 DYLVGGHSRKEVCEKYQM 70


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2935TCRTETB1298e-35 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 129 bits (325), Expect = 8e-35
Identities = 85/407 (20%), Positives = 171/407 (42%), Gaps = 16/407 (3%)

Query: 14 LMVLCLGVLMIVLDTTIVNVALPSIRADLQFSETALVWVVNAYMLTFGGFLLLGGRLGDL 73
L+ LC+ VL+ ++NV+LP I D + WV A+MLTF + G+L D
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 74 YGPRRVFLAGLTLFTAASLACGVAGSQ-QLLVAARAVQGLGGAVVSAVSLSLIMNLFSEP 132
G +R+ L G+ + S+ V S LL+ AR +QG G A A+ + +++ +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVM-VVVARYIPK 134

Query: 133 AERARAMGVYGFVCAGGGSVGVLLGGVLTSALSWHWIFLVNLPIGAAVSGLCMLLLPGGR 192
R +A G+ G + A G VG +GG++ + HW +L+ +P+ ++ ++ L +
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYI--HWSYLLLIPMITIITVPFLMKLL-KK 191

Query: 193 AAGGEARLDVAGAVSVTLSLMLAVYAVVNGNEAGWTSTHTIGLLAASAALLATFLVVESR 252
+ D+ G + +++ ++ + T++++I L S F+ +
Sbjct: 192 EVRIKGHFDIKGIILMSVGIVFFMLF---------TTSYSISFLIVSVLSFLIFVKHIRK 242

Query: 253 TSAPLMPLGLFRLRNLAIANVVGVLWAGAMFAWFFISALYMQLVLGYSAMQVGLAFLPGN 312
+ P + GL + I + G + G + + + M+ V S ++G +
Sbjct: 243 VTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPG 302

Query: 313 LIMAVCSLGVSARLVMRFGIRAPLAAGLAIAAVGLALFAQAPIDGRFALHILPGMLLLGL 372
+ + + LV R G L G+ +V + + + + +LG
Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLL-ETTSWFMTIIIVFVLGG 361

Query: 373 GAGIAFNPLLLAAMNDVPAHESGLASGVVNTAFMMGGALGLAILASL 419
+ + + + E+G ++N + G+AI+ L
Sbjct: 362 LSFTKTVISTIVSSSLKQQ-EAGAGMSLLNFTSFLSEGTGIAIVGGL 407



Score = 29.8 bits (67), Expect = 0.023
Identities = 24/107 (22%), Positives = 41/107 (38%), Gaps = 1/107 (0%)

Query: 57 MLTFGGFLLLGGRLGDLYGPRRVFLAGLTLFTAASLACG-VAGSQQLLVAARAVQGLGGA 115
++ F +GG L D GP V G+T + + L + + + V LGG
Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL 362

Query: 116 VVSAVSLSLIMNLFSEPAERARAMGVYGFVCAGGGSVGVLLGGVLTS 162
+ +S I++ + E M + F G+ + G L S
Sbjct: 363 SFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2939SACTRNSFRASE354e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 35.3 bits (81), Expect = 4e-05
Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 10/67 (14%)

Query: 70 SAWH----VVQVQVAPDHQGQGLGARLLRGVLEQA---DAAGLPAQLDVLKTN-PARRLY 121
S W+ + + VA D++ +G+G LL +E A GL L+ N A Y
Sbjct: 84 SNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGL--MLETQDINISACHFY 141

Query: 122 ERLGFRV 128
+ F +
Sbjct: 142 AKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2945IGASERPTASE280.009 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 28.5 bits (63), Expect = 0.009
Identities = 14/68 (20%), Positives = 26/68 (38%)

Query: 19 SPVAALAQAAPAAQPPTQAQPAIQPSEEQLQKFASASQKVAMVADEYRPKLQAAKDDAAR 78
+ VA Q + A EE+ + +Q+V V + PK + ++ +
Sbjct: 1083 NEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQ 1142

Query: 79 EQVYREAD 86
+ RE D
Sbjct: 1143 AEPAREND 1150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2947OMPADOMAIN701e-15 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 70.0 bits (171), Expect = 1e-15
Identities = 40/166 (24%), Positives = 70/166 (42%), Gaps = 37/166 (22%)

Query: 164 GEPPSAPKIEPEPEPTQPEPPSIAELGLDDLGDGVDVIVNEKSISFRISNELLFPSGQAV 223
G+ +AP + P P P P + F + +++LF +A
Sbjct: 192 GQGEAAPVVAPAPAP---APEVQTK-------------------HFTLKSDVLFNFNKAT 229

Query: 224 LSPAGLGLISRMAKVINR--SQGYPVSVEGHSDPVPIQTRQFPSNWELSAGRATSVLREL 281
L P G + ++ ++ + V V G++D I + + N LS RA SV+ L
Sbjct: 230 LKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDR--IGSDAY--NQGLSERRAQSVVDYL 285

Query: 282 VRDGVDPGRLRAVGYADTHPIASN--DTPQGRAA-------NRRVE 318
+ G+ ++ A G +++P+ N D + RAA +RRVE
Sbjct: 286 ISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVE 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2949HTHTETR568e-12 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.8 bits (134), Expect = 8e-12
Identities = 26/128 (20%), Positives = 46/128 (35%)

Query: 19 RDALVEATEAILAERGLEGFTLREAARRVGVSAAAPLHHFGSAAGLLTEVAILGFEALTR 78
R +++ + +++G+ +L E A+ GV+ A HF + L +E+ L +
Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72

Query: 79 HLREGARSGGNDPGARLRAQGMGYVRFALAHPARFQLMFRKDRLTDDARLAAASQAAFAE 138
E DP + LR + + + R LM + A Q A
Sbjct: 73 LELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRN 132

Query: 139 LEQAIRDY 146
L D
Sbjct: 133 LCLESYDR 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2952PF05272300.032 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.032
Identities = 10/20 (50%), Positives = 12/20 (60%)

Query: 369 FVGPSGSGKSTLVKLLLGLY 388
G G GKSTL+ L+GL
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2956TCRTETA508e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 50.2 bits (120), Expect = 8e-09
Identities = 66/290 (22%), Positives = 105/290 (36%), Gaps = 19/290 (6%)

Query: 51 GASAAQTGYLQTAQTLPFLLLSLPAGVLADRVSRRGLMTAAEALRAA--ALLGLLTLLWM 108
A G L L + G L+DR RR ++ + A A A++ LW+
Sbjct: 39 NDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWV 98

Query: 109 RALNLAWLAALGFAGAIGTVVYNVAAPALVPTLVPAARLGAANRWLELARSSAFAAGPAV 168
L + + A G GA G V A A + + ++ AGP +
Sbjct: 99 --LYIGRIVA-GITGATGAV-----AGAYIADITDGDERARHFGFMSACFGFGMVAGPVL 150

Query: 169 GGALVGTMGAPVAYMLAAALSLLAVMWLAGLPASSAPARRHQHPLRELAEGARYVATHAL 228
GG L+G + AAAL+ L + L S R L A + +
Sbjct: 151 GG-LMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGM 209

Query: 229 LRPILITAIFFNTAWFILQ---AIYVAYAIDALALDAGQVGLTLGVYGA-GMVAGALAAP 284
+ A+FF + Q A++V + D DA +G++L +G +A A+
Sbjct: 210 TVVAALMAVFF-IMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITG 268

Query: 285 CLARRLPFGGVIAAGPLAALVAAALMLGTLAWPSGLLAGAAFFLFGAGPI 334
+A RL + G +A L+ G +A L +G I
Sbjct: 269 PVAARLGERRALMLGMIADGTGYILLAFAT---RGWMAFPIMVLLASGGI 315


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2972TCRTETB764e-17 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 76.1 bits (187), Expect = 4e-17
Identities = 45/192 (23%), Positives = 84/192 (43%), Gaps = 3/192 (1%)

Query: 11 SFSDEGAPPPHVPLWLLALFTFSGTLAMHIFVPALAMAGHDLNAGNGAMQMTVSLYIIGL 70
S+S + +WL L FS M + +L +D N + + +++
Sbjct: 4 SYSQSNLRHNQILIWLCILSFFSVLNEM-VLNVSLPDIANDFNKPPASTNWVNTAFMLTF 62

Query: 71 AVGQLIYGPLSDRYGRRRVLMVGLAIYTVAGLAAALAPQVYA-LIAARLFQALGGCAGLV 129
++G +YG LSD+ G +R+L+ G+ I + + ++ LI AR Q G A
Sbjct: 63 SIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPA 122

Query: 130 LGRAMVRDTAAPSEAAKRLALMNLMVTVAPGVAPIVGGALAASLGWRAVLFVLCVLGVVN 189
L +V K L+ +V + GV P +GG +A + W + L ++ ++ ++
Sbjct: 123 LVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHW-SYLLLIPMITIIT 181

Query: 190 FMFAWRLLPETG 201
F +LL +
Sbjct: 182 VPFLMKLLKKEV 193



Score = 30.2 bits (68), Expect = 0.016
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 3/101 (2%)

Query: 76 IYGPLSDRYGRRRVLMVGLAIYTVAGLAAALAPQVYALIAARLFQALGG--CAGLVLGRA 133
I G L DR G VL +G+ +V+ L A+ + + + + G +
Sbjct: 312 IGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVIST 371

Query: 134 MVRDTAAPSEAAKRLALMNLMVTVAPGVAP-IVGGALAASL 173
+V + EA ++L+N ++ G IVGG L+ L
Sbjct: 372 IVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPL 412


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2977PF06291280.013 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 27.7 bits (61), Expect = 0.013
Identities = 11/35 (31%), Positives = 16/35 (45%)

Query: 27 FTLLTMALAAALAGCAAQRDAPESPPSAAAPATEV 61
L + ALA + GCA Q + P+A P +
Sbjct: 8 KMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETI 42


42Bpet2994Bpet3016Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet29943120.423950MarR family transcriptional regulator
Bpet29954180.950164sugE protein
Bpet29964251.645036hypothetical protein
Bpet29973160.453964curved DNA-binding protein
Bpet2998319-0.461877hypothetical protein
Bpet2999318-1.237406hypothetical protein
Bpet3000118-2.381933hypothetical protein
Bpet3001019-2.769061TetR family transcriptional regulator
Bpet3002125-3.492777GntR family transcriptional regulator
Bpet3003-126-3.043584hypothetical protein
Bpet3004-227-3.483169hypothetical protein
Bpet3005-230-4.000201hypothetical protein
Bpet3006-233-4.153516putative transcriptional regulator
Bpet3007-233-5.477332hypothetical protein
Bpet3008-234-5.836725LysR family transcriptional regulator
Bpet3009-133-7.889174tautomerase
Bpet3010-230-6.474622hypothetical protein
Bpet3011-127-6.876461putative tautomerase
Bpet3012-118-5.354754putative gluconate 5-dehydrogenase
Bpet3013013-4.173094ISRSO8-transposase orfA protein
Bpet3014110-3.707108transposase
Bpet301509-3.172711transposase
Bpet3016011-3.107417hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3001HTHTETR673e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 67.3 bits (164), Expect = 3e-16
Identities = 33/175 (18%), Positives = 61/175 (34%), Gaps = 5/175 (2%)

Query: 4 ETTRTQIMQHAQRLIQERGCNGFSYRDLAALIGIKTSSIHYYFPQKEDLLLAVVQHYHAR 63
+ TR I+ A RL ++G + S ++A G+ +I+++F K DL + + +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 64 WQATIAAIDPGLRAD--AKLRAYV-QVHQQAFCGTARICLAATL--AAELASLPQAVRQA 118
D + LR + V + R L + E V+QA
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 119 VQDFYRANEDWLACVLEQGAREGSLRVPGDLRSAAQATFAALQGSLVSARLFNNS 173
++ + D + L+ L R AA + G + + S
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQS 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3011PF03944270.008 delta endotoxin
		>PF03944#delta endotoxin

Length = 633

Score = 26.6 bits (58), Expect = 0.008
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 2/46 (4%)

Query: 29 IKGVSDLLFEVMGKPRNSTFVVIEEV--DMDSWGVGGVTVAEYRKH 72
++G LL + + N I +V + D WG+ T+ YR +
Sbjct: 166 MQGYQLLLLPLFAQAANLHLSFIRDVILNADEWGISAATLRTYRDY 211


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3012DHBDHDRGNASE811e-20 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 81.3 bits (200), Expect = 1e-20
Identities = 60/254 (23%), Positives = 103/254 (40%), Gaps = 26/254 (10%)

Query: 6 KVAIVTGASQGLGAGIVESYRKRGFAVIANSRN----LKPSSDADVVA-----VPGDIGN 56
K+A +TGA+QG+G + + +G + A N K S A P D+ +
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 57 RDVAKQLVETAISRYGRVDTLINNAGIFIAKPFTQYTVEDMDRVFRTNLHGFFHVTQFAL 116
++ G +D L+N AG+ + E+ + F N G F+ ++
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128

Query: 117 EQMLKQERGHIVQITTTLVRQAIAGLDVGLTMLTKGGLEAVTRGLAIEYAKQGIRVNAVA 176
+ M+ + G IV + + + +K T+ L +E A+ IR N V+
Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSM--AAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 177 PGIINTPMH-------DPQAHDFLGGMH------PMGRMGEIADIAKAVMYL--EEADFV 221
PG T M + G + P+ ++ + +DIA AV++L +A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 222 TGETLNVDGGQQAG 235
T L VDGG G
Sbjct: 247 TMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3013HTHFIS260.014 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 25.9 bits (57), Expect = 0.014
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3014HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


43Bpet3149Bpet3195Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet31493142.231485DNA repair protein RadC
Bpet31502132.081708hypothetical protein
Bpet31511112.187938putative tryptophan oxygenase
Bpet31520112.85596950S ribosomal protein L31
Bpet31530123.698781PMT family glycosyltransferase
Bpet31540113.503138multidrug resistance protein norM
Bpet31550124.438329hypothetical protein
Bpet31560123.874987hypothetical protein
Bpet3157-1124.128474DNA repair protein RadA
Bpet31581113.770886putative lipoprotein
Bpet31591132.973102RNA polymerase sigma factor
Bpet31600134.254926hypothetical protein
Bpet31610123.421661short chain dehydrogenase
Bpet31621113.312897catabolic alanine racemase
Bpet31630101.620317putative phytoene synthase related protein
Bpet3164-1101.845748putative phytoene synthase
Bpet31650122.276821putative oxidoreductase
Bpet3166015-0.018257hypothetical protein
Bpet3167-1142.256752transposase
Bpet31680154.113730transposase
Bpet3169-2153.627221hypothetical protein
Bpet3170-1142.733484hypothetical protein
Bpet3171-2133.377491hypothetical protein
Bpet3172-2123.487885thiamine biosynthesis lipoprotein ApbE
Bpet3173-2132.208746oxidoreductase
Bpet3174-1120.947350ATP-dependent protease, ATPase subunit
Bpet31750132.851519transcriptional regulatory protein
Bpet31761114.165423hypothetical protein
Bpet31770143.210878hypothetical protein
Bpet31781134.155214ADP-ribose pyrophosphatase
Bpet31793154.695843hypothetical protein
Bpet31801125.022383hypothetical protein
Bpet31812144.648026putative lipoprotein
Bpet31821144.139900hypothetical protein
Bpet31830133.593745AraC family transcriptional regulator
Bpet3184-1123.160441amino acid transporter LysE
Bpet3185-2122.876480hypothetical protein
Bpet3186-2112.436419hypothetical protein
Bpet3187093.691568organic hydroperoxide resistance protein
Bpet31881114.890140MarR family transcriptional regulator
Bpet31890144.008128hypothetical protein
Bpet31902153.829293hypothetical protein
Bpet31913154.014044AraC family transcriptional regulator
Bpet31922183.159368hypothetical protein
Bpet31932141.557774hypothetical protein
Bpet31942161.345288TonB-dependent outer membrane receptor
Bpet31953140.955977sulfite reductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3157PF05272300.019 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.019
Identities = 23/84 (27%), Positives = 37/84 (44%), Gaps = 6/84 (7%)

Query: 81 RVLGGGLVAGAVVLIGGDPGIGKSTLLLQALATMSAH--SRVLYVTGEESAEQVALRARR 138
RV+ G V++ G GIGKST L+ L + + TG++S EQ+ A
Sbjct: 587 RVMEPGCKFDYSVVLEGTGGIGKST-LINTLVGLDFFSDTHFDIGTGKDSYEQI---AGI 642

Query: 139 LGLQTGNVNLLAEIRLEAIQAAVS 162
+ + + EA++A S
Sbjct: 643 VAYELSEMTAFRRADAEAVKAFFS 666


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3161DHBDHDRGNASE1261e-37 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 126 bits (317), Expect = 1e-37
Identities = 77/257 (29%), Positives = 124/257 (48%), Gaps = 25/257 (9%)

Query: 1 MKDKCVLVTGATKGIGWALTQKLADLGCHVVGIARNTDHID----------FPGYLYACD 50
++ K +TGA +GIG A+ + LA G H+ + N + ++ + D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 51 LADAGRTEEVLREI-REKFPVDAIVNNVGVVRPQPLGEIDLASLYNVMDLNVRVAVQVTQ 109
+ D+ +E+ I RE P+D +VN GV+RP + + +N ++
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 110 AFVESMKVRRTGRIVNVSSRAIHGGLDRTS---YSAAKSALIGCTRTWALELAEYGVTVN 166
+ + M RR+G IV V S + RTS Y+++K+A + T+ LELAEY + N
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAG--VPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 167 AVAPGPIETELFRV--------AQPAGSDGEKRALASIPMKRLGTPAEVAAAIAFLLSDE 218
V+PG ET++ Q E IP+K+L P+++A A+ FL+S +
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTG-IPLKKLAKPSDIADAVLFLVSGQ 242

Query: 219 AGFITGQVLGVDGGGSL 235
AG IT L VDGG +L
Sbjct: 243 AGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3162ALARACEMASE379e-133 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 379 bits (976), Expect = e-133
Identities = 197/373 (52%), Positives = 239/373 (64%), Gaps = 21/373 (5%)

Query: 1 MPRPIHASISLAALAHNLDVVRRHLDQAAQAAGGAPPSIWAVIKANAYGHGIEAAVAGFS 60
M RPI AS+ L AL NL +VR+ A +W+V+KANAYGHGIE +
Sbjct: 1 MTRPIQASLDLQALKQNLSIVRQAATHAR---------VWSVVKANAYGHGIERIWSAIG 51

Query: 61 AAQGLAMLDLAEAVRCREAGWGGPILLLEGFFQPADLDLIDRYHLSATVHTREQLDMLAQ 120
A G A+L+L EA+ RE GW GPIL+LEGFF DL++ D++ L+ VH+ QL L
Sbjct: 52 ATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQN 111

Query: 121 ARLSRRVDIMLKLNSGMNRLGFDPDAYGSAHARALQLREQGVVGAVGRMTHFACADGTPG 180
ARL +DI LK+NSGMNRLGF PD QLR VG + M+HFA A+ G
Sbjct: 112 ARLKAPLDIYLKVNSGMNRLGFQPD---RVLTVWQQLRAMANVGEMTLMSHFAEAEHPDG 168

Query: 181 VAGQLRVFQSVTQGLADGPVSVCNSAATLRYPEIAVAHGAQAHWVRPGICLYGASPFAD- 239
++G + + +GL + S+ NSAATL +PE A WVRPGI LYGASP
Sbjct: 169 ISGAMARIEQAAEGL-ECRRSLSNSAATLWHPE------AHFDWVRPGIILYGASPSGQW 221

Query: 240 ADAASFGLRPAMSLRSQIIGVQDLPAGAEVGYGATFRAERPMRVGVVACGYADGYPRHAG 299
D A+ GLRP M+L S+IIGVQ L AG VGYG + A R+G+VA GYADGYPRHA
Sbjct: 222 RDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAP 281

Query: 300 TGTPVVVGGVRTRLVGRVSMDMLMVDLDPVPAAGIGTPVSLWGQDGPSVDEVAQAAGTIG 359
TGTPV+V GVRT VG VSMDML VDL P P AGIGTPV LWG+ +D+VA AAGT+G
Sbjct: 282 TGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGK-EIKIDDVAAAAGTVG 340

Query: 360 YELLCALAPRVPV 372
YEL+CALA RVPV
Sbjct: 341 YELMCALALRVPV 353


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3167HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3174HTHFIS382e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 37.5 bits (87), Expect = 2e-04
Identities = 42/196 (21%), Positives = 70/196 (35%), Gaps = 34/196 (17%)

Query: 561 EEIAEVVSRATGIPVAKMMQGEREKLLQMEDHLHKRVVGQDEAVRLVSDAIRRSRAGLAD 620
E+ ++ RA P + + E + M +VG+ A++ + + R L
Sbjct: 109 TELIGIIGRALAEPKRRPSKLEDDSQDGMP------LVGRSAAMQEIYRVLAR----LMQ 158

Query: 621 PSRPYGSFLFLGPTGVGKTELTRALADFLFDSEEHMIRIDMSEFMEKHSVARLIGAPPGY 680
+ G +G GK + RAL D+ + I+M+ + L
Sbjct: 159 TDLT---LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESEL------- 208

Query: 681 VGYEEGGYLTEAVRRKPYSV-------VLLDEVEKAHPDVFNVLLQVLDDG---RLTDGQ 730
G+E+G + T A R + LDE+ D LL+VL G +
Sbjct: 209 FGHEKGAF-TGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRT 267

Query: 731 GRTVDFRNTVIVMTSN 746
D R IV +N
Sbjct: 268 PIRSDVR---IVAATN 280


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3189SACTRNSFRASE300.003 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 29.9 bits (67), Expect = 0.003
Identities = 12/52 (23%), Positives = 25/52 (48%), Gaps = 6/52 (11%)

Query: 84 IAVAPDCQGRGIGSMLVIEGLARLRGRGAAGC------VVLGRPHYYSRFGF 129
IAVA D + +G+G+ L+ + + + G + + H+Y++ F
Sbjct: 95 IAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3192TCRTETB300.014 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 30.2 bits (68), Expect = 0.014
Identities = 21/96 (21%), Positives = 36/96 (37%), Gaps = 4/96 (4%)

Query: 275 FFAGALVKRWGLPTMLGLGMAINVAS---AGVAIASTSLPAFYAALFFLGVGWNFMFVGG 331
+ G LV R G +L +G+ S A + +TS +F LG G +F
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSFTKTVI 369

Query: 332 TTLLAQSYRPAERGRAQGAAEMLRYAATALATLAAG 367
+T+++ S + E G + + G
Sbjct: 370 STIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVG 405


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3195PF07520290.047 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 29.2 bits (65), Expect = 0.047
Identities = 20/96 (20%), Positives = 26/96 (27%), Gaps = 1/96 (1%)

Query: 240 PPGVRVALPMSDEHLGHTGPTTWSLEQARMPESAYAHAMGQPSQPIGLDAAVAAFDRLGL 299
+ P H + E R A + AA+ F +
Sbjct: 42 RSFRFIERPEGAAEGRHRTLYPLTGEAERDAPILAATTPEDDEYSVRPLAALEPFLEKWV 101

Query: 300 -APGYAINVPHGAAGVYTGSVYPSDLARQRVVHLDQ 334
P + GA G PS AR R V L Q
Sbjct: 102 PIPVLRLKNQRGAGGEELYDPGPSSWARLRTVELPQ 137


44Bpet3208Bpet3213Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet32082120.480493acyl-CoA transferase/carnitine dehydrastase
Bpet32093130.940732spermidine synthase (putrescine
Bpet32105131.732615putative DNA/RNA endonuclease
Bpet32116122.345880protein-S-isoprenylcysteine O-methyltransferase
Bpet32126142.990950hypothetical protein
Bpet32134132.823236putative cytochrome c
45Bpet3240Bpet3245Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3240210-0.385386hypothetical protein
Bpet3241212-0.874891hypothetical protein
Bpet3242310-0.111776cytochrome C oxidase subunit
Bpet3243310-0.507924hypothetical protein
Bpet32444110.271676cbb3-type cytochrome c oxidase subunit II
Bpet32454150.325962cbb3-type cytochrome c oxidase subunit I
46Bpet3267Bpet3291Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet32672140.259222molybdenum transporter
Bpet3268113-0.834147Sulfate/thiosulfate import ATP-binding protein
Bpet3269-113-0.780466putative transmembrane component of ABC
Bpet3270-212-0.247875hypothetical protein
Bpet3271-2130.668087putative TRAP-type C4-dicarboxylate transport
Bpet3272-2131.475944putative TRAP-type C4-dicarboxylate transport
Bpet3273-1142.085141putative TRAP-type C4-dicarboxylate transport
Bpet3274-3143.390176malonyl-CoA synthase
Bpet3275-1114.577135hypothetical protein
Bpet3276-1114.471376putative malonyl-CoA decarboxylase
Bpet3277-2114.065596GntR family transcriptional regulator
Bpet3278-2124.092293hypothetical protein
Bpet3279-1133.961124hypothetical protein
Bpet32800133.654512hypothetical protein
Bpet3281-2113.119395putative acyl-CoA dehydrogenase
Bpet3282-1112.787645putative secreted protein
Bpet3283-1102.154086putative Acetyl-CoA synthetase
Bpet3284-2110.873853enoyl-CoA hydratase
Bpet32850101.156311IclR family transcriptional regulator
Bpet32860122.466531beta-ketothiolase
Bpet32870112.959192enoyl-CoA hydratase
Bpet32881123.392057transposase
Bpet32891144.202305transposase
Bpet32900153.653795putative acyl-CoA dehydrogenase
Bpet32910163.742321putative acyl-CoA dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3289HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


47Bpet3327Bpet3343Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet33273122.180893gamma-glutamyl phosphate reductase
Bpet33285150.792698putative ABC transport proteins, inner membrane
Bpet33297170.960353LysR family transcriptional regulator
Bpet33307141.492559methylenomycin A resistance protein
Bpet33312121.410393hypothetical protein
Bpet33320111.579828hypothetical protein
Bpet33330101.370311hypothetical protein
Bpet33350111.764979**hypothetical protein
Bpet3336-1121.627748LysR family transcriptional regulator
Bpet3337-1131.677781hypothetical protein
Bpet33380151.526373GntR family transcriptional regulator
Bpet33392161.494402periplasmic mannitol-binding protein
Bpet33404171.485532TRAP-type dicarboxylat transporter, small
Bpet33414161.338698TRAP dicarboxylate transporter
Bpet33423142.1686463-hydroxy-3-methylglutaryl-coenzyme A reductase
Bpet33434201.816934putative integral membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3330TCRTETB1088e-28 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 108 bits (272), Expect = 8e-28
Identities = 78/408 (19%), Positives = 164/408 (40%), Gaps = 19/408 (4%)

Query: 24 LLALAIGCVMAMLDVTVVNVALPSIGAQLGTPLSGLVWIIDGYTLAFAALLLAAGALSDH 83
L+ L I ++L+ V+NV+LP I P + W+ + L F+ G LSD
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 84 YGARPVYLAGLALFTLASLLCGAAPST-GLLVTARLLQGVGAALFMPSSLSLLMHAYQVP 142
G + + L G+ + S++ S LL+ AR +QG GAA F P+ + +++ Y
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAF-PALVMVVVARYIPK 134

Query: 143 EVRGRMLAAWSAIITVAATAGPLAGGMLIDLFGWRSIFLINLPL-GLAGLWLARAHLESP 201
E RG+ +I+ + GP GGM+ W +L+ +P+ + + L+
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKE 192

Query: 202 AARPRPLNPGNHLLGIGMLGLASYALIQG-NVYGWTAPRIVTAGVLALACGAALLARERR 260
+ + GI ++ + + Y + ++++ + R+
Sbjct: 193 VRIKGHFD----IKGIILMSVGIVFFMLFTTSYSISFL------IVSVLSFLIFVKHIRK 242

Query: 261 HPHPIVPRALAQTPGYLATNTYGFLVSFSVYGLIFLLSLYVQQALGADALQAGLKLLPVF 320
P V L + ++ G ++ +V G + ++ ++ + G ++
Sbjct: 243 VTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPG 302

Query: 321 GVFSI-GNLAAGRLAVRWGARATMLVGAAIGALAAIATAILCAPDSPYLLLVILLGVGNL 379
+ I G L R G + +G +++ + + L S ++ ++I+ +G L
Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL 362

Query: 380 ATGAAIPAMTALALQIGGAAHANSAAAALNANRQAGALVGVAAIGGVL 427
+ + ++ + A + + LN G+A +GG+L
Sbjct: 363 SFTKTV--ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3332SECA300.003 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.003
Identities = 9/15 (60%), Positives = 9/15 (60%)

Query: 12 CPCGSGLAYSACCER 26
CPCGSG Y C R
Sbjct: 885 CPCGSGKKYKQCHGR 899


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3337CHANLCOLICIN382e-04 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 37.7 bits (87), Expect = 2e-04
Identities = 35/150 (23%), Positives = 54/150 (36%), Gaps = 18/150 (12%)

Query: 53 ATMAVSAIAQPEHPPERPPDAAEAESALADARKQIDEIRKHLEDGGEDAQLVQWRADVLD 112
AT S + E+ A A A A A+ D + + L+D +A R +
Sbjct: 53 ATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEA----LRHNASR 108

Query: 113 IQSRAD-ALAEALAPQLASMTARLTELGEPPAGTREAPDVAAQRAQLQKSSRALDSQTKL 171
S + A A A Q RL + E EA + A Q A+ ++ ++
Sbjct: 109 TPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRK--------EI 160

Query: 172 ARLLSVEAAQTAEQIS-TLRRNQFQARLGE 200
R E A+T Q+ + A L E
Sbjct: 161 ER----EKAETERQLKLAEAEEKRLAALSE 186


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet33392FE2SRDCTASE290.029 Ferric iron reductase signature.
		>2FE2SRDCTASE#Ferric iron reductase signature.

Length = 262

Score = 28.8 bits (64), Expect = 0.029
Identities = 10/28 (35%), Positives = 14/28 (50%)

Query: 256 YESLPAPYKAALAAAARETSAALRQHIL 283
+ + P LA A R T A R+H+L
Sbjct: 14 WRTHLQPQDPTLAQAVRATIAKHREHLL 41


48Bpet3405Bpet3411Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet34050133.965461putative inositol monophosphatase family
Bpet34060124.725763NAD-dependent deacetylase
Bpet3407-2133.772301hypothetical protein
Bpet3408-2143.788466TetR family transcriptional regulator
Bpet3409-1154.073974acyl-CoA dehydrogenase
Bpet3410-1154.084858acetyl-CoA acetyltransferase
Bpet3411-2153.339145enoyl-CoA hydratase / 3-hydroxyacyl-CoA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3408HTHTETR682e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 68.5 bits (167), Expect = 2e-16
Identities = 30/111 (27%), Positives = 51/111 (45%), Gaps = 5/111 (4%)

Query: 1 MPAMNELNSPSTREIILDTAEALFARQGHDGTSMRQITSEAGVNLAAVNYHFGSKEALVQ 60
M + + TR+ ILD A LF++QG TS+ +I AGV A+ +HF K L
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 AVLKRRLAVLNQERLRLLDELEARAGGEPLK-PSQIVDAFFGTLLRLAARP 110
+ + + + + L E +A+ G+PL +I+ + + R
Sbjct: 61 EIWELSESNIGE----LELEYQAKFPGDPLSVLREILIHVLESTVTEERRR 107


49Bpet3477Bpet3488Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet34772170.792519hypothetical protein
Bpet34783170.995510hypothetical protein
Bpet34791170.744772hypothetical protein
Bpet34801220.456740hypothetical protein
Bpet34813240.349253hypothetical protein
Bpet3482221-0.524873hypothetical protein
Bpet3483214-0.048859putative glycosyltransferase
Bpet34841130.082337hypothetical protein
Bpet3485012-0.064919putative lipoprotein
Bpet34861120.667767AraC family transcriptional regulator
Bpet34871110.373344short chain dehydrogenase
Bpet34883140.716485*putative membrane-associated phospholipase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3485VACJLIPOPROT260.005 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 26.0 bits (57), Expect = 0.005
Identities = 11/27 (40%), Positives = 12/27 (44%)

Query: 2 LCALTVFACLLGGCAVYTPDGAVIVDP 28
L AL + LL GCA D DP
Sbjct: 5 LSALALGTTLLVGCASSGTDQQGRSDP 31


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3487DHBDHDRGNASE872e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 86.6 bits (214), Expect = 2e-22
Identities = 64/259 (24%), Positives = 111/259 (42%), Gaps = 20/259 (7%)

Query: 4 VSLVTGAGRGLGRNTALSIARRGGDVIITYRSGKDAAEGVVADVHALGRKAVALQLDTAE 63
++ +TGA +G+G A ++A +G + + E VV+ + A R A A D +
Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 64 VASFPTFVHAVRAALRQNWQRET--FDHLVNNAGHGEMADFAATTEAQFDALFNVHVKGV 121
A+ + +RE D LVN AG + ++ +++A F+V+ GV
Sbjct: 69 SAAI--------DEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 122 FFLTQALLPLLAD--GGRIVNLSSGLTRVSFPGFSAYSAAKGAVETLTVYMAKELGSRGI 179
F ++++ + D G IV + S V +AY+++K A T + EL I
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 180 TANAVAPGAIETDFL-------GGAVRDTPDYNKAFADITALGRVGVPDDIGPMIANLLS 232
N V+PG+ ETD GA + + F L ++ P DI + L+S
Sbjct: 181 RCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240

Query: 233 ADNRWVNGQRIEVSGGQSI 251
+ + V GG ++
Sbjct: 241 GQAGHITMHNLCVDGGATL 259


50Bpet3549Bpet3555Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet35490143.971938putative ABC transporter, substrate binding
Bpet35500105.498108facyl-CoA transferase/carnitine dehydratase
Bpet3551195.421698recombination protein RecR
Bpet3552294.947778hypothetical protein
Bpet3553195.022593DNA polymerase III subunits gamma and tau
Bpet3554194.495608putative nuclease/helicase
Bpet3555-1103.054679hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3553PF03544389e-05 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 37.6 bits (87), Expect = 9e-05
Identities = 20/123 (16%), Positives = 28/123 (22%), Gaps = 3/123 (2%)

Query: 391 PATALQAPAAATPRPEAVAATAASPAAPAAAAPVASATAAPVAQAAAAAPPPPAAVQPRA 450
P + A P+AV P P P
Sbjct: 49 PISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPV 108

Query: 451 GAASGAAPAAPAVPVAQTA---NPAPQAQGAAPAAPVSSAPAAQPAAADTPPWEDLPATP 507
V + N AP ++ A +S P A+ + P P
Sbjct: 109 KKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYP 168

Query: 508 AAA 510
A A
Sbjct: 169 ARA 171



Score = 35.3 bits (81), Expect = 6e-04
Identities = 24/132 (18%), Positives = 34/132 (25%), Gaps = 10/132 (7%)

Query: 417 APAAAAPVASATAAPVAQAAAAAPPPPAAVQPRAGAASGAAPAAPAVPVAQTANPAPQAQ 476
AP + VA A PP AVQP P P+ + AP
Sbjct: 40 VIELPAPAQPISVTMVAPADL---EPPQAVQPP--PEPVVEPEPEPEPIPEPPKEAPVVI 94

Query: 477 GAAPAAPVSSAPAAQPAAADTPPWEDLPATPAAAEPAAAAKAPPAAALAVTPPAAQAKAP 536
P + + + + PA+ A P ++ A P
Sbjct: 95 EKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSS-----TATAATSKP 149

Query: 537 PPDADGEPPAWV 548
P A
Sbjct: 150 VTSVASGPRALS 161


51Bpet3570Bpet3593Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3570-2143.046259benzoyl-CoA-dihydrodiol lyase
Bpet3571-2142.842865anaerobic benzoate catabolism transcriptional
Bpet35720162.622725hypothetical protein
Bpet35731183.130111aldehyde dehydrogenase
Bpet35741202.492656acyl-coenzyme A synthetase
Bpet35752213.203416hypothetical protein
Bpet35762182.443926putative ABC transporter substrate binding
Bpet35774163.260074putative branched-chain amino acid transport
Bpet35783153.348226branched-chain amino acid transport system,
Bpet35792152.852836putative branched-chain amino acid ABC
Bpet35801142.152477putative branched amino acid ABC transporter
Bpet35811132.408111TetR family transcriptional regulator
Bpet35822153.536637outer membrane efflux protein
Bpet35832153.746264MFS family transporter
Bpet35840123.682907secretion protein
Bpet35850123.787538LysR family transcriptional regulator
Bpet35861144.396328hypothetical protein
Bpet35870144.007494MFS permease
Bpet3588-2153.923699LysR family transcriptional regulator
Bpet35890154.000247putative secreted protein
Bpet35901154.733720putative 3-oxoadipate CoA-transferase subunit A
Bpet35912144.633043putative 3-oxoadipate CoA-transferase subunit B
Bpet35922154.625406IclR family transcriptional regulator
Bpet35931174.053261putative translation factor
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3581HTHTETR661e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 66.2 bits (161), Expect = 1e-15
Identities = 37/186 (19%), Positives = 64/186 (34%), Gaps = 17/186 (9%)

Query: 26 AVLTAAREVFLTHGFSAATTDMIQRAAGVSKATVYAYYPTKQALFEAVIEGKCAEHM--A 83
+L A +F G S+ + I +AAGV++ +Y ++ K LF + E ++
Sbjct: 15 HILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE-LSESNIGEL 73

Query: 84 TLRSLRSVPGAIHAVLSELANAYLEFGVAPEGLALFRV-------SAAEAPRFPELARAF 136
L PG +VL E+ LE V E L E + R
Sbjct: 74 ELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNL 133

Query: 137 FENGPEAYCGIVAEHVERGVRNQELHLGDVSPQEAARLFFSLVRGQAQLEGVLLPDRRPS 196
+ + +E L D+ + AA + + G +E L +
Sbjct: 134 CLESYDRIEQTLKHCIEAK----MLPA-DLMTRRAAIIMRGYISG--LMENWLFAPQSFD 186

Query: 197 EAQKKR 202
++ R
Sbjct: 187 LKKEAR 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3582RTXTOXIND310.017 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.017
Identities = 27/155 (17%), Positives = 52/155 (33%), Gaps = 8/155 (5%)

Query: 70 LDALVARAWDGNLDLQAAAARVEQSRARAGVALAQL--FPRVDLDASLTRGAISENGPMA 127
L AL A A AR+EQ+R + +L P + L +SE +
Sbjct: 127 LTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLR 186

Query: 128 ALGAPTSTTDLWRAGFQADWEIDLWGRLRRQREGAVATLQATLYEQRSAQVALSAE---I 184
W+ + E++L + R +R +A + R + L +
Sbjct: 187 LTSLIKEQFSTWQNQ-KYQKELNL-DKKRAERLTVLARINRYENLSRVEKSRLDDFSSLL 244

Query: 185 ARQYVA-LRGVQTRLDIARRNQEIAAHLLRLTETR 218
+Q +A ++ E+ + +L +
Sbjct: 245 HKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIE 279


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3583TCRTETB381e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 37.6 bits (87), Expect = 1e-04
Identities = 68/411 (16%), Positives = 144/411 (35%), Gaps = 40/411 (9%)

Query: 37 INNRVGALALADIRGAGGFGLDDASWITTAYTAGELIAMPLAPWFAVTLSLRRFHL--QM 94
+N V ++L DI +W+ TA+ I + + L ++R L +
Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87

Query: 95 LAAGAAIAGVLPFVHDLRLLLLLRGLQGVASGALIPLLMMAALRFFPPSIRLFALALYSM 154
+ ++ G + LL++ R +QG + A L+M+ R+ P R A L
Sbjct: 88 INCFGSVIGFVGHSF-FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGS 146

Query: 155 TATFAPNVSTWLTGLWTDQLVDLRMVYWQIVPINLLAGLLVAWGIPQDRPLPERFRHANW 214
V + G+ + Y ++P+ + + + + R
Sbjct: 147 IVAMGEGVGPAIGGMIAHY---IHWSYLLLIPMITIITVPFLMKLL-----KKEVRIKGH 198

Query: 215 LGMAFGGAGLLLLAIGIEQGNRLEWFTSPLVCTSLSAGSLLL---AFYLFTEWHHPT--P 269
+ G++L+++GI L TS S L++ +F +F + P
Sbjct: 199 FDI----KGIILMSVGI--------VFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDP 246

Query: 270 FIKLQLLKRRNLWLGFSLFLCLLVIFLSGSLLPATLLGHAWHYRALQSAPIGLMIGLPQL 329
F+ L K +G + + ++ L +A IG +I P
Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQ---LSTAEIGSVIIFP-G 302

Query: 330 VVAPAVAMLLYQKWVDARA---VMAAGMAITAAACLLGAQVTNQWMWPEFALAQGLQAVG 386
++ + + VD R V+ G+ + + L + + W + + V
Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSW---FMTIIIVFVL 359

Query: 387 QPMAIVAMLF--LATSMVAPQEGPYVSGIVNLLRALGAPLGSALISRVIEL 435
++ + + +S + QE ++N L G A++ ++ +
Sbjct: 360 GGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSI 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3584RTXTOXIND1061e-27 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 106 bits (266), Expect = 1e-27
Identities = 50/415 (12%), Positives = 118/415 (28%), Gaps = 84/415 (20%)

Query: 4 SKKTKLAGSATVMVAAVALAL-IFNRPESAAATQSTDDAYIRAEITSVAPEITGLVEAVL 62
S++ +L + +A L + + E A + P +V+ ++
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGK--LTHSGRSKEIKPIENSIVKEII 111

Query: 63 VEENQPVRAGQLLV---------------------------------------------- 76
V+E + VR G +L+
Sbjct: 112 VKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLP 171

Query: 77 ------QIDDREYVLAERNAAAALAHARAAADGIHAQIEVQQSVIRQAQSTIEADQATRE 130
+ + E + + + ++ +++ + I +
Sbjct: 172 DEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSR 231

Query: 131 LARLDYSRYKSLAADGSGTVQARQQAKARL---------------QVEKAQQTKDQAILQ 175
+ + + SL + A + + + Q+E + +
Sbjct: 232 VEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQL 291

Query: 176 AQKGRQAALQADLLRAQAEIRQAEAALAQARLDLSRTRITAPIAGTIGHKRVR-VGNYAR 234
+ + + L + I LA+ + I AP++ + +V G
Sbjct: 292 VTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVT 351

Query: 235 TGDPLLTLVPLTDIY-IEANFRETQLARMRQGQPVRVTVDALPGRTF---TGTVQSLGPA 290
T + L+ +VP D + A + + + GQ + V+A P + G V+++
Sbjct: 352 TAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLD 411

Query: 291 SGVSYSVIAPHNATGNFTKIVQRLPVRIALDPQQDGADQLRVGMSVQPEVDVNAR 345
+ G ++ + + L GM+V E+ R
Sbjct: 412 A-------IEDQRLGLVFNVIISIEENCLS--TGNKNIPLSSGMAVTAEIKTGMR 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3587TCRTETB290.028 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.5 bits (66), Expect = 0.028
Identities = 31/173 (17%), Positives = 62/173 (35%), Gaps = 3/173 (1%)

Query: 37 VMNLFAVQTVAPVIAASLGLGLDSVGVLAMLPQLGYALGLVLLVPLADRLENRRLIGATL 96
V+N + P IA S + L +++G + L+D+L +RL+ +
Sbjct: 27 VLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGI 86

Query: 97 AVCALCMLAAAFAPGGA--VFMAAVFAGGASTCAIQMLVPMAAFMAAPERRGATVGNVMS 154
+ + + MA G + +++ + A E RG G + S
Sbjct: 87 IINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGS 146

Query: 155 GLMVGVLLSRPLSNLVVDAWGWRALYLVFAGGMAATGVALLCLLPQRRPHDGP 207
+ +G + + ++ W L L+ + T L+ LL + G
Sbjct: 147 IVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITI-ITVPFLMKLLKKEVRIKGH 198


52Bpet3603Bpet3614Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3603-1173.064450translation initiation factor IF-1
Bpet36040163.492611hypothetical protein
Bpet36050163.644024hypothetical protein
Bpet3606-1133.211462hypothetical protein
Bpet36070142.404668putative secreted protein
Bpet36081142.496516LysR family transcriptional regulator
Bpet36092122.658975putative peptidase
Bpet36101142.135930putative lipoprotein
Bpet36111152.451174molybdenum cofactor biosynthesis protein MogA
Bpet36122151.942757phosphate transporter
Bpet36133133.722257hypothetical protein
Bpet36141143.925922hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3609PF00577290.027 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 29.0 bits (65), Expect = 0.027
Identities = 23/99 (23%), Positives = 34/99 (34%), Gaps = 9/99 (9%)

Query: 5 RLSLRPRLGRVAGRLVLLAAACAVAAAAHAQG----YISRKLDVPVPGGVAVVALGTAER 60
L R R+AG V L ACA AA A + R L AV L E
Sbjct: 13 TQCLHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLAD---DPQAVADLSRFEN 69

Query: 61 APQASYGGHRVMVLRDSDGQWIAVVGIALDAKPGRHTLQ 99
+ G +RV + + ++A + + +
Sbjct: 70 GQELPPGTYRVDIY--LNNGYMATRDVTFNTGDSEQGIV 106


53Bpet3629Bpet3643Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3629-2113.285124hypothetical protein
Bpet3630-3132.231277aminotransferase
Bpet3631-1151.650081hypothetical protein
Bpet3632-1161.109937putative transglycosylase
Bpet36330143.7493823-octaprenyl-4-hydroxybenzoate carboxy-lyase
Bpet36340153.953861hypothetical protein
Bpet3635-2152.343724hypothetical protein
Bpet3636-1142.394412hypothetical protein
Bpet3637-2153.139939hypothetical protein
Bpet3638-2154.189536nitrate reductase, catalytic subunit
Bpet3639-3162.650594nitrite reductase (NAD(P)H) small subunit
Bpet3640-3163.071914assimilatory nitrite reductase large subunit
Bpet3641-3155.305820hypothetical protein
Bpet3642-3174.638029putative response regulator NasT
Bpet3643-3183.626619siroheme synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3636cloacin260.035 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 25.8 bits (56), Expect = 0.035
Identities = 15/64 (23%), Positives = 27/64 (42%), Gaps = 1/64 (1%)

Query: 19 KSSLNDAENLLREAASSSGDKATELRDRAMASLKRTREALYDAQDAVLERGRKAARATDD 78
++ +N+ + AA D L AM S K+ + A++ + + K + D
Sbjct: 401 QTDVNNKQAAFDAAAKEKSDADAAL-SSAMESRKKKEDKKRSAENNLNDEKNKPRKGFKD 459

Query: 79 YVHD 82
Y HD
Sbjct: 460 YGHD 463


54Bpet3686Bpet3795Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet36863192.907055formate dehydrogenase accessory protein
Bpet36874193.150441NAD-dependent formate dehydrogenase delta
Bpet36882193.202983MFS permease
Bpet36890154.081347putative methylase
Bpet36900133.929354putative chromate transport protein
Bpet36910134.471901putative chromate transporter
Bpet36921154.892496hypothetical protein
Bpet36930163.463525putative short chain dehydrogenase
Bpet3694-112-0.964735hypothetical protein
Bpet3695-116-2.084164transmembrane regulator
Bpet3696024-4.947815RNA polymerase sigma factor
Bpet3697032-6.567111putative secreted protein
Bpet3698038-8.081934putative nucleotide-binding protein
Bpet3699042-8.524321phage-related integrase
Bpet3700234-7.003923hypothetical protein
Bpet3701235-7.494119conjugal transfer protein TrbM
Bpet3702234-8.134394type IV secretion system protein VirB1
Bpet3703236-8.927825conjugal transfer protein TrwL
Bpet3704137-9.010760type IV secretion system protein VirB3
Bpet3705137-8.947848type IV secretion system protein VirB4
Bpet3706144-10.038092hypothetical protein
Bpet3707140-9.068701hypothetical protein
Bpet3708136-7.466624type IV secretion system protein VirB5
Bpet3709033-6.560172type IV secretion system protein VirB6
Bpet3710030-5.753284putative conjugal transfer protein TrwH
Bpet3711130-5.722266type IV secretion system protein VirB8
Bpet3712132-6.433043type IV secretion system protein VirB9
Bpet3713233-6.310550type IV secretion system protein VirB10
Bpet3714234-7.788804type IV secretion system protein VirB11
Bpet3715234-8.034451TrfA-related protein
Bpet3716231-7.347009hypothetical protein
Bpet3717120-7.012313hypothetical protein
Bpet3718219-6.425950hypothetical protein
Bpet3719122-6.527387type IV secretion system protein VirD4
Bpet3720023-5.932878transposase
Bpet3721123-5.848640transposase
Bpet3722124-6.406055ISSod9, transposase
Bpet3723236-6.579708transcriptional regulator
Bpet3724237-6.874919glutathione reductase
Bpet3725134-6.321891glutathione S-transferase
Bpet3726133-5.760416glutathione S-transferase family protein
Bpet3727033-5.117295glutathione S-transferase family protein
Bpet3728-131-3.022989MarR family transcriptional regulator
Bpet3729029-3.140032hypothetical protein
Bpet3730-128-3.387577maleylacetate reductase
Bpet3731126-3.032719putative enolase
Bpet3732125-2.863051hypothetical protein
Bpet3733121-3.527744putative transposase
Bpet3734320-4.539409muconate cycloisomerase
Bpet3735319-4.377271carboxymethylenebutenolidase
Bpet3736321-2.938124putative transposase
Bpet3737320-2.6875002-hydroxy-6-phenylhexa-2,4-dienoic acid
Bpet3738321-4.565420large terminal subunit of phenylpropionate
Bpet3739328-5.427613chlorobenzene dioxygenase, small subunit of
Bpet3740037-7.334952phenylpropionate dioxygenase ferredoxin subunit
Bpet3741140-7.918653putative ferredoxin reductase
Bpet3742240-8.5534502,3-dihydroxy-2,3-dihydrophenylpropionate
Bpet3743239-8.530594ring hydroxylating alpha subunit
Bpet3744336-6.624970ring hydroxylating beta subunit
Bpet3745335-5.608462putative transport protein
Bpet3746430-3.577709AraC-type transcriptional regulator
Bpet3747530-2.278653transcriptional regulator catR
Bpet3748530-2.863941catechol 1,2-dioxygenase
Bpet3749433-3.142332chloromuconate cycloisomerase
Bpet3750433-3.334767hypothetical protein
Bpet3751333-3.662350carboxymethylenebutenolidase
Bpet3752232-2.746148maleylacetate reductase
Bpet3753228-1.322975hypothetical protein
Bpet3754327-1.167224putative branched-chain amino acid ABC
Bpet3755228-1.545802putative branched-chain amino acid transporter,
Bpet3756228-1.479796branched chain amino acid ABC transporter
Bpet3757327-2.094280hypothetical protein
Bpet3758325-2.153382putative transposase
Bpet3759325-3.878495putative ATPase fragment
Bpet3760224-3.669928hypothetical protein
Bpet3761123-4.105897hypothetical protein
Bpet3762222-4.127946ISPpu15, transposase Orf2
Bpet3763321-3.834113putative ATPase fragment
Bpet3764128-6.118566hypothetical protein
Bpet3765130-6.554427hypothetical protein
Bpet3766035-7.446584Tn3 family transposase
Bpet3767036-8.168014transposase
Bpet3768038-8.563078transposase
Bpet3769044-9.572913conjugal transfer protein
Bpet3770149-10.848779hypothetical protein
Bpet3771350-11.530614single-strand binding protein
Bpet3772251-11.387007hypothetical protein
Bpet3773253-11.351401DNA repair protein radC-like protein
Bpet3774153-10.825386cytochrome c-type biogenesis protein CcmF
Bpet3775244-9.134467thiol:disulfide interchange protein DsbE
Bpet3776241-8.220392cytochrome C-type biogenesis protein
Bpet3777141-7.230654cytochrome C-type biogenesis protein
Bpet3778038-7.087463hypothetical protein
Bpet3779-136-6.996438acyl-CoA dehydrogenase
Bpet3780-133-7.260520transposase
Bpet3781036-8.271447transposase
Bpet3782038-8.604428acyl-CoA dehydrogenase
Bpet3783034-8.172711IclR family transcriptional regulator
Bpet3784031-7.970806citrate synthase
Bpet3785132-7.752435acyl dehydratase
Bpet3786133-7.683574acyl-CoA dehydrogenase
Bpet3787234-7.361928putative short chain dehydrogenase
Bpet3788233-6.950209acetyl-CoA synthetase
Bpet3789232-6.960368putative secreted protein
Bpet3790132-6.788220hypothetical protein
Bpet3791038-7.060908hypothetical protein
Bpet3792239-7.157772electron transfer flavoprotein beta-subunit
Bpet3793243-7.527425electron transfer flavoprotein alpha-subunit
Bpet3794045-7.288415acyl-CoA dehydrogenase fragment
Bpetpseudo_10141-7.405895hypothetical protein
Bpetpseudo_11036-6.193880hypothetical protein
Bpet3795-121-4.485700putative transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3693DHBDHDRGNASE993e-27 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 99.0 bits (246), Expect = 3e-27
Identities = 68/254 (26%), Positives = 107/254 (42%), Gaps = 25/254 (9%)

Query: 5 KVALITAGGSGMGAAAARKLAADGYRV-AILSSSGKGEALASELQGLGVTGSNLEPDDIA 63
K+A IT G+G A AR LA+ G + A+ + K E + S L+ P D+
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF-PADVR 67

Query: 64 --RLVDAAMQRW----GRVDAVVNSAGHGPKGKLLDIPDADWHLGMEYYLLNVVRITRLV 117
+D R G +D +VN AG G + + D +W V +R V
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 118 APIMRQQRGGSIVNISTYATFEPEALFPTSGVFRAGLAAFTKVFADEYAADNVRMNNVLP 177
+ M +R GSIV + + P +A FTK E A N+R N V P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 178 GFIDS------LPEKDDRRQR-----------IPMGRYGRADEVAELIAFLASDKSGYIT 220
G ++ +++ Q IP+ + + ++A+ + FL S ++G+IT
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHIT 247

Query: 221 GQNIRIDGGITRSV 234
N+ +DGG T V
Sbjct: 248 MHNLCVDGGATLGV 261


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3702PF05616300.005 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 30.5 bits (68), Expect = 0.005
Identities = 22/60 (36%), Positives = 29/60 (48%), Gaps = 10/60 (16%)

Query: 155 VPALAPLKNEMTGPATKATPAE-PAQKPDQGPPAQPEGAPDGFSANPATDGFSQTRDGEP 213
+P ++P +N PA P E P +P+ P P+ PD ANP TDG TR P
Sbjct: 328 LPEVSPAEN----PANNPAPNENPGTRPN--PEPDPDLNPD---ANPDTDGQPGTRPDSP 378


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3705CLENTEROTOXN320.006 Clostridium enterotoxin signature.
		>CLENTEROTOXN#Clostridium enterotoxin signature.

Length = 319

Score = 32.3 bits (73), Expect = 0.006
Identities = 16/113 (14%), Positives = 39/113 (34%), Gaps = 15/113 (13%)

Query: 644 LHDGRRIINLYDECQHPLKDRHFQEDMQDASRTIRKKNGVLAFATQEPGAITENP----- 698
+ + + + ++ + P+ + ++ D I K +G + + NP
Sbjct: 11 FENAKEVFLISEDLKTPINITNSNSNLSDGLYVIDKGDGWILGEPSVVSSQILNPNETGT 70

Query: 699 VGPSLVQQTATLILLPNPRAKARDYIE-----GFGL-----SPTEFELLKSLG 741
SL + I + ++I+ GFG+ + E + + G
Sbjct: 71 FSQSLTKSKEVSINVNFSVGFTSEFIQASVEYGFGITIGEQNTIERSVSTTAG 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3711PF043351732e-56 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 173 bits (440), Expect = 2e-56
Identities = 73/231 (31%), Positives = 111/231 (48%), Gaps = 7/231 (3%)

Query: 1 MSASLKSEDVAAYLEQSRGLERDHLGELVSSRKRAWQVAIGAGLIALASVAAVAGLTPLK 60
M+ + +++ AY E++ ERD L S+K AW VA AG +A A V AVA LTPLK
Sbjct: 1 MAVGIPKDELKAYFEEAASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLK 60

Query: 61 QPPEMYVVRVDSATGSIEHVSSLGQPLED-YGQRIAKYFLNTYVLNCEGYSWQTIQEQFD 119
E YV+ VD TG + L Y + + KYFL TYV EG+ +E FD
Sbjct: 61 T-VEPYVITVDRNTGEASIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFD 119

Query: 120 TCALLSSAPIQTQYGKRFEGQ--DAVTTRLGTQGTVDVQVHSITLGANQAAIVRFTKTER 177
++S+ P Q ++ + ++ + L + V V++ ++ A V FTK
Sbjct: 120 AVMVMSARPEQDRWSRFYKTDNPQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESV 179

Query: 178 EVSTGNITKAQHLIATMAYQYTDVPLTEEVARLNPLGFQVMRYDLAADLSR 228
TG+ + +AT+ Y+ P E NPLG+QV Y ++ +
Sbjct: 180 ---TGSNSTKTDAVATIKYKVDGTPSKEVDRFKNPLGYQVESYRADVEVPQ 227


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3712MPTASEINHBTR320.002 Metalloprotease inhibitor signature.
		>MPTASEINHBTR#Metalloprotease inhibitor signature.

Length = 122

Score = 31.5 bits (71), Expect = 0.002
Identities = 21/65 (32%), Positives = 26/65 (40%), Gaps = 14/65 (21%)

Query: 142 AQAAQKAAIERSLQAVSANMNWQAYTRSGDAAIAPVHAWDDGRQTWLQFAPQADIPTVYR 201
AQ A + IE + V A QA +GD A A + WL D P +
Sbjct: 34 AQMAGQLGIEATGSGVCAGPAEQANALAGDVACA---------EQWL-----GDKPVSWS 79

Query: 202 VTPDG 206
TPDG
Sbjct: 80 PTPDG 84


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3713PF03544361e-04 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 36.1 bits (83), Expect = 1e-04
Identities = 12/85 (14%), Positives = 22/85 (25%)

Query: 69 PAAKADSGLEADQSGITNRLKAPEVERPAPPPALPPPTPEPTVAPNYNVPSPMPAAPPPV 128
P + E + + +E+P P P P + P +V P
Sbjct: 70 PEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPF 129

Query: 129 DELTQRRLASPLQAGGADASGATPS 153
+ R S + +
Sbjct: 130 ENTAPARPTSSTATAATSKPVTSVA 154



Score = 35.7 bits (82), Expect = 2e-04
Identities = 22/132 (16%), Positives = 41/132 (31%), Gaps = 6/132 (4%)

Query: 43 RAFLW---LTILIAVAVAAGVLMKVWSREPAAKADSGLEADQSGITNRLKAPEVERPAPP 99
R F W L++ I AV AG+L + A + + + + PP
Sbjct: 12 RRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPA--QPISVTMVAPADLEPPQAVQPP 69

Query: 100 PA-LPPPTPEPTVAPNYNVPSPMPAAPPPVDELTQRRLASPLQAGGADASGATPSQSNGP 158
P + P PEP P +P+ P + + ++ D ++
Sbjct: 70 PEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPF 129

Query: 159 QGPYSDAGPLAD 170
+ +
Sbjct: 130 ENTAPARPTSST 141


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3719HTHFIS290.038 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.4 bits (66), Expect = 0.038
Identities = 11/33 (33%), Positives = 19/33 (57%)

Query: 128 ANENLHLLITGATGSGKSVLLRNMAASVLRRSR 160
+L L+ITG +G+GK ++ R + RR+
Sbjct: 157 MQTDLTLMITGESGTGKELVARALHDYGKRRNG 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3720HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3742DHBDHDRGNASE652e-14 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 65.1 bits (158), Expect = 2e-14
Identities = 60/245 (24%), Positives = 101/245 (41%), Gaps = 19/245 (7%)

Query: 3 LKGEVALVTGGGAGLGRAIVDRYVAEGARVAVLDKSAAGLEEIRK------RHGDAVVGI 56
++G++A +TG G+G A+ ++GA +A +D + LE++ RH +A
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEA---F 62

Query: 57 EGDVRSLDSHREAVARCVETFGKLDCLIGNAGVWDYLTQLADIPDNGISEAFDEMFAINV 116
DVR + E AR G +D L+ AGV + + D E ++ F++N
Sbjct: 63 PADVRDSAAIDEITARIEREMGPIDILVNVAGVL-RPGLIHSLSD----EEWEATFSVNS 117

Query: 117 KGYILAAKAALPALYKSKGSAIFTV-SNAGFYPGGGGVLYTAGKHAVIGLVKQLAHEWGP 175
G A+++ + + +I TV SN P Y + K A + K L E
Sbjct: 118 TGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAE 177

Query: 176 R-IRVNGIAPGGILGSDLRGLKT-LGLQDQTIATMPLADMLGPVLPTGRVATAEEYAGAY 233
IR N ++PG L +Q I G +P ++A + A A
Sbjct: 178 YNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTG--IPLKKLAKPSDIADAV 235

Query: 234 VFFAT 238
+F +
Sbjct: 236 LFLVS 240


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3745TCRTETB359e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.9 bits (80), Expect = 9e-04
Identities = 21/95 (22%), Positives = 39/95 (41%), Gaps = 7/95 (7%)

Query: 110 LGAIIFGRFGDKIGRKTTFLITIVMIGSATVGMGLLPTFASIGWWAPILLTLLRVMQGLA 169
+G ++G+ D++G K L I++ +V + +F S+ L + R +QG
Sbjct: 64 IGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSL-------LIMARFIQGAG 116

Query: 170 LGGEYGGAAAYVAEYSEPKRRGLTTGFLQATAALA 204
VA Y + RG G + + A+
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMG 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3754PF05272280.045 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 27.7 bits (61), Expect = 0.045
Identities = 11/22 (50%), Positives = 14/22 (63%)

Query: 36 VVTLLGRNGMGKTTTIRSLVGA 57
V L G G+GK+T I +LVG
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3759HTHFIS270.045 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.7 bits (59), Expect = 0.045
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 1/33 (3%)

Query: 50 MIHGEPGTGKSVVMRVLAEKLERLTDLTVVSIN 82
MI GE GTGK +V R L + +R + V+IN
Sbjct: 164 MITGESGTGKELVARALHDYGKR-RNGPFVAIN 195


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3765RTXTOXIND310.004 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.004
Identities = 22/176 (12%), Positives = 62/176 (35%), Gaps = 5/176 (2%)

Query: 76 NTQLVQQVADLQERLAAEERHRQSLEEKHKHARQALEHFRESTKEQRDQDQRKHEQQVQY 135
++ + L + + R++ + L+ E + +++
Sbjct: 133 EADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTS--L 190

Query: 136 LQAELRTVNETLATKQQEAVHTLQENARLLGDLSRAQGDLHQAQEEVRGLRPLKDELGFA 195
++ + T K+ E +L ++R + + + L + A
Sbjct: 191 IKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIA 250

Query: 196 QRRSEELGRRLVEQDAAVQQLSTSKEQLQAKVDELLSAKQQLELALATARSSVTAQ 251
+ E + VE ++ + EQ+++ E+LSAK++ +L ++ + +
Sbjct: 251 KHAVLEQENKYVEAVNELRVYKSQLEQIES---EILSAKEEYQLVTQLFKNEILDK 303


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3767HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3781HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3787DHBDHDRGNASE1356e-41 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 135 bits (340), Expect = 6e-41
Identities = 85/252 (33%), Positives = 124/252 (49%), Gaps = 11/252 (4%)

Query: 5 IRGKVALVTGSGRGIGAEASRQLAQEGARVVICDIDVETADATAQNLRDEGFEAMAIQCD 64
I GK+A +TG+ +GIG +R LA +GA + D + E + +L+ E A A D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 65 VCDKDQVQAGIDAVVKEWGGVDILVNNAGFTRDKYLTKMSEEDWDSVVDTILKGAFHFSR 124
V D + + +E G +DILVN AG R + +S+E+W++ G F+ SR
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 125 AVLPGMMERKWGRIVNIASRSVFGNP--GQTNYTTAKLGLVGFTRALALEQARFGITVNA 182
+V MM+R+ G IV + S G P Y ++K V FT+ L LE A + I N
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPA-GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 183 IAPGFIETELMRSLPTYPELREMALARN--------PVGFLGAPEDIASSVAFLSSEHAR 234
++PG ET++ SL E + + P+ L P DIA +V FL S A
Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAG 244

Query: 235 YITGITLYVTGG 246
+IT L V GG
Sbjct: 245 HITMHNLCVDGG 256


55Bpet3811Bpet3829Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet38110113.033318GntR family transcriptional regulator
Bpet38122113.431342putative secreted protein
Bpet38131103.102283fumarate hydratase, class I
Bpet3814-2111.302214TetR family transcriptional regulator
Bpet3815-1111.058473hypothetical protein
Bpet38162100.158288putative 2-pyrone-4,6-dicarboxylic acid
Bpet3817112-1.054492D-3-phosphoglycerate dehydrogenase
Bpet3818011-1.185501transcriptional regulator
Bpet3819113-1.815584C4-dicarboxylate-binding periplasmic protein
Bpet3820215-0.790227TRAP-type C4-dicarboxylate transport system,
Bpet3821-114-1.125660TRAP-type C4-dicarboxylate transport system,
Bpet3822016-0.901616putative nitrite extrusion protein
Bpet3823-115-0.633975peptidyl-prolyl cis-trans isomerase
Bpet3824-214-0.172457nitrate reductase gamma chain
Bpet3825-2131.019955nitrate reductase delta chain
Bpet3826-2121.432298nitrate reductase beta chain
Bpet3827-1102.040641nitrate reductase 1, alpha chain
Bpet38281103.462751hypothetical protein
Bpet38290103.367394putative 2-nitropropane dioxygenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3814HTHTETR588e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.1 bits (140), Expect = 8e-13
Identities = 29/171 (16%), Positives = 57/171 (33%), Gaps = 8/171 (4%)

Query: 6 TREQLVCHAQALIRQRGYNGFSYRDLAERVGVKTASIHYYFPCKDDLLIEAIDSYAQHVA 65
TR+ ++ A L Q+G + S ++A+ GV +I+++F K DL E + ++
Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71

Query: 66 GLVHGIDATLPA------KERLDRY-AALFEGGPTDQVCLCGMLAADFASLSDRARKSLQ 118
L A P +E L + + +F +++ +
Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQR 131

Query: 119 GFFCLHETWLAKVIADGQRDGTLQWAGCPDAAGRCLFAAFQGALMGSRLFQ 169
+ + + L A + G LM + LF
Sbjct: 132 NLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISG-LMENWLFA 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3822TCRTETA508e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 50.2 bits (120), Expect = 8e-09
Identities = 65/337 (19%), Positives = 118/337 (35%), Gaps = 29/337 (8%)

Query: 43 AFQFGLLTSTPVLTGAVFRLPLGMWTDRYGGRAVMTVLLVGCAIPVYLVSYAQALWQFLL 102
+G+L + L LG +DR+G R V+ V L G A+ +++ A LW +
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYI 101

Query: 103 IGLFLGLVGASFAVGTPYVARFFSAERKGFAMGFFGAGTVGAAVNLFVTPILLETYGWRA 162
+ G+ GA+ AV Y+A + + GF A V V L+ + A
Sbjct: 102 GRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHA 161

Query: 163 VPKIYAVALLVTAALFWFVAAPDPGAGKAGGKLLDQFKILKN--------PRVWRYCQYY 214
P A AL L P+ G+ + L + ++
Sbjct: 162 -PFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFF 220

Query: 215 SITFGGFTALSLWIPQYFQAEYGLSLVAASALAAGFSLPGAVLRA-VGGSLADRYGAHKM 273
+ G +LW+ + + + A F + ++ +A + G +A R G +
Sbjct: 221 IMQLVGQVPAALWV-IFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRA 279

Query: 274 TWWCLWLAWICLFILSYPNTTLTVQTLNGSAAFHIYLPIWLFTLLLFVLGAMFAFGMAST 333
+ +A +IL AF + ++L G + + +
Sbjct: 280 LMLGM-IADGTGYILL---------------AFATRGWMAFPIMVLLASGGIGMPALQAM 323

Query: 334 FKYVADDFPENMGVVTGIVGLAGGLGGFLLPLMFGAM 370
D+ E G + G + L + PL+F A+
Sbjct: 324 LSRQVDE--ERQGQLQGSLAALTSLTSIVGPLLFTAI 358


56Bpet3924Bpet3960Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3924215-3.013360hypothetical protein
Bpet3925114-1.982342LysR family transcriptional regulator
Bpet3926112-1.661561two component response regulator
Bpet3927212-1.663920hypothetical protein
Bpet3928212-1.264681two component response regulator
Bpet3929-111-0.214427NADH dehydrogenase
Bpet3930-2110.338611serine/threonine kinase protein
Bpet3931-2201.605226co-chaperonin GroES
Bpet3932-1172.471914chaperonin GroEL
Bpet39332152.800387BrkB transmembrane protein
Bpet39343142.672524xanthine dehydrogenase yagR molybdenum binding
Bpet39351123.543976xanthine dehydrogenase
Bpet39362102.271689putative xanthine dehydrogenase
Bpet3937192.394107putative malate dehydrogenase
Bpet39382101.985085transposase
Bpet39391123.071412transposase
Bpet39402132.443674hypothetical protein
Bpet39412140.636659mandelate racemase/muconate lactonizing protein
Bpet3942-115-0.029862UxaA family hydrolase
Bpet3943-1150.0122382-hydroxyhepta-2,4-diene-1, 7-dioateisomerase /
Bpet3944-114-0.207573oxidoreductase
Bpet3945-114-0.831654TRAP C4-dicarboxylate transport system, large
Bpet3946-3140.644027TRAP-type C4-dicarboxylate transport system,
Bpet3947-3141.464450C4-dicarboxylate-binding periplasmic protein
Bpet3948-1123.141160L-idonate 5-dehydrogenase
Bpet3949-2142.844417GntR family transcriptional regulator
Bpet3950-1124.110086hypothetical protein
Bpet3951-1144.288852NADH dehydrogenase
Bpet3952-1173.628507orotidine 5'-phosphate decarboxylase
Bpet3953-1203.867152competence/damage inducible protein CinA
Bpet3954-1193.232257phosphatidylglycerophosphatase A
Bpet3955-1203.041483thiamine monophosphate kinase
Bpet3956-1142.799900transcription antitermination protein NusB
Bpet3957-1143.0173386,7-dimethyl-8-ribityllumazine synthase
Bpet3958-1143.076822bifunctional 3,4-dihydroxy-2-butanone
Bpet39590143.060862fumarylacetoacetate hydrolase family protein
Bpet39602133.319078hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3926HTHFIS695e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 69.1 bits (169), Expect = 5e-17
Identities = 20/110 (18%), Positives = 46/110 (41%), Gaps = 6/110 (5%)

Query: 7 VAIVDDDESIRHATDSLVRSFRR---RTLVFASAEDFLQSGKLAETSCLISDIMMPGMSG 63
+ + DDD +IR L ++ R + ++A + + +++D++MP +
Sbjct: 6 ILVADDDAAIR---TVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 64 LEMHKRMRMLGYAPPTIFITAYPAADLTAQAMASGALAVLEKPVEADAIA 113
++ R++ P + ++A +A GA L KP + +
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELI 112


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3928HTHFIS1111e-30 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 111 bits (278), Expect = 1e-30
Identities = 32/148 (21%), Positives = 64/148 (43%)

Query: 21 VYIVDDDESMRLSLQSLLRSSGLRVETFQSAQEFLAFPKSQGPSCLVLDVRLRGESGLAF 80
+ + DDD ++R L L +G V +A + + +V DV + E+
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 81 QEQIAKSGLRMPIVFMTGHGDIAMTVKAMKAGAVDFLAKPFRDQDMLDAVANALARDSER 140
+I K+ +P++ M+ +KA + GA D+L KPF +++ + ALA R
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 141 LAAEQSIAQLRAAHASLTPREREVMALV 168
+ + +Q + +E+ ++
Sbjct: 126 PSKLEDDSQDGMPLVGRSAAMQEIYRVL 153


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3930YERSSTKINASE320.022 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 32.4 bits (73), Expect = 0.022
Identities = 40/179 (22%), Positives = 71/179 (39%), Gaps = 24/179 (13%)

Query: 112 DKLHQHGLIHKDIKPAHILVHCTDGRARFTGFGLASRLPRERQAPTPPETIAGTLAYMAP 171
+ L + G++H DIKP +++ G GL SR + + T ++ AP
Sbjct: 259 NHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSGEQPKGFTE--------SFKAP 310

Query: 172 EQTGRMNRSVDSRSDLYALGVTFYQMLTG------VLPFSAMDAMEWVHCHIARTPMPAA 225
E G N +SD++ + T + G + P + + H+
Sbjct: 311 E-LGVGNLGASEKSDVFLVVSTLLHCIEGFEKNPEIKPNQGLRFITSEPAHVMDENGYPI 369

Query: 226 ER--VATVPAAISRIVMKLLAKTAEDRYQTAAGLEHDLRRCLADWNRLGRIDEFSLDEL 282
R +A V A +R + +L +A+ R + H+ L+D G IDE S ++
Sbjct: 370 HRPGIAGVETAYTRFITDILGVSADSRPDSNEARLHEF---LSD----GTIDEESAKQI 421


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3939HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3944DHBDHDRGNASE1241e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (312), Expect = 1e-36
Identities = 76/253 (30%), Positives = 118/253 (46%), Gaps = 14/253 (5%)

Query: 5 LEGKTAFVTAAGQGIGRATALAFAREGATVLAADLNPQALGGLGECKPVT--------LD 56
+EGK AF+T A QGIG A A A +GA + A D NP+ L + D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 57 VTDAAAV----ARAVEVAGPVDILFNGAGFVHAGTILDCDDAAWDFSFDLNVRAMYRLIR 112
V D+AA+ AR GP+DIL N AG + G I D W+ +F +N ++ R
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 113 AFLPGMLARGGGSIINMASAASSVKGVPNRFVYGTTKAAVIGLTKSVAADFVGRGIRCNA 172
+ M+ R GSI+ + S + V + Y ++KAA + TK + + IRCN
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRT-SMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 173 ICPGTVESPSLRQRIAEQARASGQTEQAVEAAFVARQPMGRIGRAEEIAALALYLASDES 232
+ PG+ E+ A++ + Q + F P+ ++ + +IA L+L S ++
Sbjct: 185 VSPGSTETDMQWSLWADE-NGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 233 AFTTGTAQIIDGG 245
T +DGG
Sbjct: 244 GHITMHNLCVDGG 256


57Bpet3978Bpetpseudo_14Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3978-1123.287520hypothetical protein
Bpet39790113.584505MarR family transcriptional regulator
Bpet39800113.336520autotransporter
Bpet3981-1120.859283transcriptional regulatro, PadR-like
Bpet3982116-0.680526iron utilization protein
Bpet3983019-2.296153quinone oxidoreductase
Bpet3984133-8.912190LysR family transcriptional regulator
Bpet3985135-10.421883putative transposase
Bpet3986229-9.896905transposase
Bpet3987329-10.037390transposase
Bpet3988429-10.226204transposase
Bpet3989228-9.410613type I restriction-modification system, S
Bpet3990217-7.224747hypothetical protein
Bpet3991319-7.424112type I restriction modification enzyme M
Bpetpseudo_13219-7.662073hypothetical protein
Bpet3992112-6.197140transposase
Bpet3993012-4.313149transposase
Bpetpseudo_14-211-3.943211type I restriction-modification enzyme R
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3980FLAGELLIN378e-04 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 37.3 bits (86), Expect = 8e-04
Identities = 32/279 (11%), Positives = 68/279 (24%), Gaps = 6/279 (2%)

Query: 1490 AQSIGGGGGLAGAGSANNLTSVTLGGRNGATGDGGAVSLALNAGSVISTTGAGAH--ALV 1547
+S+G G + + +N D AV V S V
Sbjct: 164 VKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTV 223

Query: 1548 AQSIGGGGGIAGDTAQAVQLDASHWQPSGTNSGGSAVTGGSGNGSAVTVDVNGSIVTSGA 1607
+ T + + + T S + G+ + G
Sbjct: 224 PDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGV 283

Query: 1608 GAFGILAQSIGGGGGLGGGLVPDGAGDGGTISQGFAGSTGGSGSGAEVTVTQAGSIVVAG 1667
G G + + +G T++ AG+ + + + S+V
Sbjct: 284 TFTIDTKTGNDGNGKVSTTI--NGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQ 341

Query: 1668 AGSTGIFAQSAGGDGAGAVTVNVNGSVTGGSGSSGYGVWVASPAQNVLNVGADGQIIAGQ 1727
+ V G + Y A + A
Sbjct: 342 FTFDDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASG 401

Query: 1728 GGAAVRHDGAALSGAGAALAPAGAATLAINNAGSIRGNI 1766
+ + A + + P + A++ ++R ++
Sbjct: 402 VSTLI--NEDAAAAKKSTANPLASIDSALSKVDAVRSSL 438


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3992HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


58Bpet4003Bpet4024Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet40030153.191197*4-diphosphocytidyl-2-C-methyl-D-erythritol
Bpet4004-1183.436693outer membrane lipoprotein LolB
Bpet4005-1183.495119hypothetical protein
Bpet4006-1203.663440formamidopyrimidine-DNA glycosylase
Bpet40070183.787711putative cyclase
Bpet40080184.559882FAD-dependent oxidoreductase
Bpet40091155.046379hypothetical protein
Bpet40100154.482225LysR family transcriptional regulator
Bpet40110143.226339haloacid dehalogenase
Bpet4012-1153.148981putative secreted protein
Bpet4013-2142.955869hypothetical protein
Bpet4014-2132.901240putative endoribonuclease
Bpet4015-2122.640051LysR family transcriptional regulator
Bpet4016-2142.889578homogentisate 1,2-dioxygenase
Bpet4017-2143.124739fumarylacetoacetase
Bpet4018-1143.415956putative two-component system sensor protein
Bpet4019-1143.602518two-component system response regulator
Bpet40200123.732356putative oxidoreductase
Bpet40210123.604954hypothetical protein
Bpet40222144.072284glucose-6-phosphate isomerase
Bpet40233164.336639glycosyltransferase
Bpet40241133.470909putative glycosyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4005SYCDCHAPRONE406e-06 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 40.3 bits (94), Expect = 6e-06
Identities = 17/83 (20%), Positives = 32/83 (38%), Gaps = 1/83 (1%)

Query: 445 ATLEAANQAQPDTVEIKYELAMLYERQGRYDELETQLRQVIALDPDHAHAYNALGYTLAD 504
T+ N+ DT+E Y LA + G+Y++ + + LD + + LG
Sbjct: 23 GTIAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQA 82

Query: 505 RNQRLPEALDLITQALELQPDDP 527
Q A+ + + +P
Sbjct: 83 MGQ-YDLAIHSYSYGAIMDIKEP 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4010BLACTAMASEA290.030 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 28.6 bits (64), Expect = 0.030
Identities = 19/81 (23%), Positives = 32/81 (39%), Gaps = 12/81 (14%)

Query: 17 PSLSATARALNVTPPALSMRLRKLEAALG-----LALAVRTARRLSLTPEGERFAR---- 67
SL AT P +++ E+ L + + + + R L+ ERF
Sbjct: 9 ISLLATLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTF 68

Query: 68 ---EAAALLAQLQALPESLQR 85
A+LA++ A E L+R
Sbjct: 69 KVVLCGAVLARVDAGDEQLER 89


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4013ALARACEMASE431e-06 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 42.8 bits (101), Expect = 1e-06
Identities = 32/188 (17%), Positives = 62/188 (32%), Gaps = 18/188 (9%)

Query: 16 AIIDVARMQANIDRMQQRMNTLGVALRPHVKTSK----CTPVAQAQLAAGARGITVSTLK 71
A +D+ ++ N+ ++Q + VK + + A A + L+
Sbjct: 7 ASLDLQALKQNLSIVRQAA--THARVWSVVKANAYGHGIERIWSAIGATDGFA--LLNLE 62

Query: 72 EAEQFFEAGI-ADILYAVGMV-PQRLPAALALRRRGCDLKIITDNAAAARAIAAFGREHG 129
EA E G IL G Q L R + ++ A A
Sbjct: 63 EAITLRERGWKGPILMLEGFFHAQDLEIYDQHR---LTTCVHSNWQLKALQNARLKA--- 116

Query: 130 EVFEVWIEIDTDGHRSGIKPGEAALLEVGAALHEGGMTLGGVMTHAGSSYDLDTPAALAA 189
++++++++ +R G +P L + +M+H + D + A
Sbjct: 117 -PLDIYLKVNSGMNRLGFQPDR-VLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMA 174

Query: 190 MAEQERAG 197
EQ G
Sbjct: 175 RIEQAAEG 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4015PF07520300.013 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 29.9 bits (67), Expect = 0.013
Identities = 26/166 (15%), Positives = 47/166 (28%), Gaps = 27/166 (16%)

Query: 24 ISAVARRLDLSQPAVSNALRRLRATLGDDLFVRTPQGMQPTPQAERLGGPVGEALALLSH 83
I+ A R SQ + L R+ +L P + + V AL L+
Sbjct: 477 INDPASRSRRSQSDLPRRLNRVILSL--------PTAT-SVQEQAMIRSRVSGALTLVKE 527

Query: 84 TLEATQDFHPADSQRRFRIAMS-DVGEIHFMPRLMEQCARHAPAIRIDSLRLAGADLRRE 142
L + + + + D + L + RID+ R +
Sbjct: 528 MLGTKDGTSTIAVEGKPELLVDWDEASCTQLVYLYSE-LTQKFDGRIDTFLDLKGQPRPD 586

Query: 143 MDAGRV---------------DLAIGAFEDLGS-GIMQRMLFRQGY 172
G DL + + + + FR+G+
Sbjct: 587 PAGGESPSLRLACIDVGGGTTDLMVTTYRGEDNRVLHPEQTFREGF 632


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4019HTHFIS815e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 81.4 bits (201), Expect = 5e-20
Identities = 38/138 (27%), Positives = 65/138 (47%), Gaps = 4/138 (2%)

Query: 9 RYRVLVVEDDLTIAGNLYRFLEVNGFVPDVAYDGRTALRMLQDQRFDAMVLDVGLPGLDG 68
+LV +DD I L + L G+ + + T R + D +V DV +P +
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 69 YQVLQTLRAERRQAIPVLILTARDALDDKLEGFSHGADDYLTKPFALAEVQA---RLLAL 125
+ +L ++ + R +PVL+++A++ ++ GA DYL KPF L E+ R LA
Sbjct: 63 FDLLPRIK-KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 126 IHRAQGAVVDAVREFGPL 143
R + D ++ PL
Sbjct: 122 PKRRPSKLEDDSQDGMPL 139


59Bpet4130Bpet4138Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4130015-3.278564outer membrane usher protein
Bpet4131-218-4.593044pili assembly chaperone
Bpet4132-217-4.703272type-1 fimbrial protein
Bpet4133-117-4.965168hypothetical protein
Bpet4134-116-4.494306AraC family transcriptional regulator
Bpet4135-118-5.838241hypothetical protein
Bpet4136-217-5.556240hypothetical protein
Bpet4137-315-3.985465hydrolase
Bpet4138-316-3.116606putative amino acids ABC transporter ATP-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4130PF005776910.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 691 bits (1786), Expect = 0.0
Identities = 282/855 (32%), Positives = 426/855 (49%), Gaps = 45/855 (5%)

Query: 1 MAACSALSIAQEQGGDAAMFDESFLYRAPGQAGGVDLSVFAYSSRVLPGSKSVLLQLNER 60
+ A + F+ FL P DLS F + PG+ V + LN
Sbjct: 30 LFVACAFAAQAPLSSAELYFNPRFLADDPQ--AVADLSRFENGQELPPGTYRVDIYLNNG 87

Query: 61 AIGNRVVEFIVVPGKEDAQPCYSVAALRELGVKVEAFPELQKMDENECGRALEIIPSAKA 120
+ R V F ++ PC + A L +G+ + + + ++ C +I A A
Sbjct: 88 YMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATA 147

Query: 121 SYDQDANILRLSIPQAALGRQARGVVPVERWDSGTTALWSSYRMSYNHMRSTGGPGSYSN 180
D L L+IPQA + +ARG +P E WD G A +Y S N +++ ++
Sbjct: 148 QLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN---RIGGNS 204

Query: 181 DTLYLGFRNGLNLGAWRIRGNGSY------YENGYSTDWDWSDLYAERDIVSWRGRLRLG 234
YL ++GLN+GAWR+R N ++ +G W + + ERDI+ R RL LG
Sbjct: 205 HYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLG 264

Query: 235 DSATEGRIFDSVRFRGIQLRSDDGMLPDSQRGYAPVIRGIAPSNAKVTVRQNGYVLYTTF 294
D T+G IFD + FRG QL SDD MLPDSQRG+APVI GIA A+VT++QNGY +Y +
Sbjct: 265 DGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNST 324

Query: 295 VPAGPFVIDDLYSTPGGGDLEVEIDEMGGRTTRYFQPFSALPTMMREGIWNYNFMVGEHR 354
VP GPF I+D+Y+ GDL+V I E G T + P+S++P + REG Y+ GE+R
Sbjct: 325 VPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYR 384

Query: 355 HNYD-TSRPLMGQLTLAYGLPWGLTAYGGWTLAQHGYHAGAFGLAANLRHLGAVSADITS 413
+P Q TL +GLP G T YGG LA Y A FG+ N+ LGA+S D+T
Sbjct: 385 SGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLAD-RYRAFNFGIGKNMGALGALSVDMTQ 443

Query: 414 SRSRDVRGNSMSGSAVSVQYAKSFPGSGTDFTLASYRYNSSGYRSLNDVVRDRAEQYD-- 471
+ S + G +V Y KS SGT+ L YRY++SGY + D R Y+
Sbjct: 444 ANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIE 503

Query: 472 ----------------YIGYDREHEYQLSMQQRLGRMGSLSFNYYGIAYRNAPRNARYAQ 515
+ Y++ + QL++ Q+LGR +L + Y Q
Sbjct: 504 TQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQ 563

Query: 516 VGYSSSLGRLGYSLNYALSRSPWNAREST-LMLTLSIPLGGSH-----------TASYAM 563
G +++ + ++L+Y+L+++ W L L ++IP +ASY+M
Sbjct: 564 AGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSM 623

Query: 564 NRTDNQGTNHSVSLSGAMLDDYSLTYALQAGVTRGEGQDNGNTGYGSLGYSSPVGLATVS 623
+ N + + G +L+D +L+Y++Q G G ++G+TGY +L Y G A +
Sbjct: 624 SHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIG 683

Query: 624 HAYSRNSSNTYLDISGSILADSKGVLFGQSLGETAVIVEAPGAAGVAIDALPGVRTNSAG 683
+++S + Y +SG +LA + GV GQ L +T V+V+APGA ++ GVRT+ G
Sbjct: 684 YSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRG 743

Query: 684 RALVPYASPYRENRISLDAGEGLDGAHLKQNVQTVVPTRGAIVVAKFDTEIGRTVLAVLK 743
A++PYA+ YRENR++LD D L V VVPTRGAIV A+F +G +L L
Sbjct: 744 YAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLT 803

Query: 744 DGSGRVVPFGAAVHGDDGRQRGIVGPVGRAWLTGLQGMQRFTAKWGEQGDQQCSFEIDVS 803
+ + +PFGA V + + GIV G+ +L+G+ + KWGE+ + C +
Sbjct: 804 H-NNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLP 862

Query: 804 ADQDAAG-QAKELIC 817
+ C
Sbjct: 863 PESQQQLLTQLSAEC 877


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4136ECOLIPORIN300.007 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 29.9 bits (67), Expect = 0.007
Identities = 14/42 (33%), Positives = 22/42 (52%)

Query: 50 GLEYDPELLYLGAMFHDMGLTQPYASTDLRFEVDGANAARDF 91
GL+YD +YL M+ + PY TD ++ AN ++F
Sbjct: 251 GLKYDANNIYLATMYSETRNMTPYGKTDKGYDGGVANKTQNF 292


60Bpet4154Bpet4190Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4154014-3.884145hypothetical protein
Bpet4155118-3.307380putative lipoprotein
Bpet4156018-3.300886autotransporter
Bpet4157019-4.616856LuxR family transcriptional regulator
Bpet4158-118-4.433712hypothetical protein
Bpet4159019-4.078407hypothetical protein
Bpet4160019-2.730504putative enoyl-CoA hydratase
Bpet4161019-3.420565acyl-CoA transferase/carnitine dehydratase
Bpet4162119-3.468341putative secreted protein
Bpet4163119-3.858561LysR family transcriptional regulator
Bpet4164119-3.745407hypothetical protein
Bpet4165017-3.222355biotin sulfoxide reductase
Bpet4166015-3.556836putative secreted protein
Bpet4167-213-3.332741putative oxidoreductase
Bpet4168-213-4.000201dihydrodipicolinate synthase
Bpet4169-217-3.840421cystathionine gamma-lyase
Bpet4170-117-4.039655NAD(P) transhydrogenase subunit alpha
Bpet4171018-5.543317NAD(P) transhydrogenase subunit beta
Bpet4172123-6.722247LysR family transcriptional regulator
Bpet4173225-7.291144AsnC family transcriptional regulator
Bpet4174123-6.928534LysR family transcriptional regulator
Bpet4175119-6.626163hypothetical protein
Bpet4176-122-6.348592DNA-dependent ATPase, SNF2 family protein
Bpet4177-131-6.412954hypothetical protein
Bpet4178-228-5.511422hypothetical protein
Bpet4179-128-5.622809ISPssy, transposase
Bpet4180134-5.0424743-oxoacid CoA-transferase subunit B
Bpet4181137-5.498321hypothetical protein
Bpet4182231-4.091338IS4 family transposase
Bpet4183221-3.882904putative transposase
Bpet4184321-3.264037HTH-type transcriptional regulator AcrR family
Bpet4185320-2.903321TetR family transcriptional regulator
Bpet4186220-3.284756hypothetical protein
Bpet4187219-3.504775outer membrane efflux protein
Bpet4188222-4.454231AcrB/AcrD/AcrF family protein
Bpet4189122-4.184700multidrug resistance protein
Bpet4190019-3.401925TetR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4154PF07132320.006 Harpin protein (HrpN)
		>PF07132#Harpin protein (HrpN)

Length = 356

Score = 32.0 bits (72), Expect = 0.006
Identities = 16/43 (37%), Positives = 19/43 (44%)

Query: 450 GMVALAKLQKSSSSSSGGSGSSGGGSSGGSFGGGSSGGGGAGG 492
M + + GG GSS GG GG GGG GG G+
Sbjct: 58 MMFMGSMMGGGLGGGLGGLGSSLGGLGGGLLGGGLGGGLGSSL 100



Score = 30.4 bits (68), Expect = 0.015
Identities = 14/33 (42%), Positives = 15/33 (45%)

Query: 460 SSSSSSGGSGSSGGGSSGGSFGGGSSGGGGAGG 492
GG GSS G G + GGG G GAG
Sbjct: 88 LGGGLGGGLGSSLGSGLGSALGGGLGGALGAGM 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4156cloacin340.003 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 33.9 bits (77), Expect = 0.003
Identities = 27/86 (31%), Positives = 35/86 (40%), Gaps = 8/86 (9%)

Query: 51 GSGGAGGGDGTDGYISGGNGGSDFFLPGAGGAAGVPLPTQTIP-GTADGSSLLPSYEYVG 109
G G G T G I+GG G G G + G ++ P G GS + + G
Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLG---VGGGASDGSGWSSENNPWGGGSGSGI----HWGG 58

Query: 110 IGGGGGGGDGGDLGGLHGEVGGAGAI 135
G G GG G+ GG G G A+
Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAV 84



Score = 33.1 bits (75), Expect = 0.005
Identities = 36/117 (30%), Positives = 47/117 (40%), Gaps = 11/117 (9%)

Query: 52 SGGAGGGDGTDGYISGGN--GGSDFFLPGAGGAAGVPLPTQTIP-GTADGSSLLPSYEYV 108
SGG G G T + + GN GG G G + G ++ P G GS +
Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-------I 54

Query: 109 GIGGGGGGGDGGDLGGLHGEVGGAGAINLDGTALNVD-NTLFVGGAGGGGGSGGAGS 164
GGG G G+GG G G G G ++ + L GAGG S AG+
Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111



Score = 33.1 bits (75), Expect = 0.006
Identities = 28/97 (28%), Positives = 37/97 (38%), Gaps = 4/97 (4%)

Query: 157 GGSGGAGSTRGGTGGAGGAGTLTATGGATITVGTQLYIGGLPGAGGNCGCSGNGGAGGAG 216
GG G G G + G+G + GG + G G G GG G SG G G
Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGS-GSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80

Query: 217 VFNLGDGSTLNLTGAAFTINGAGTLNIGSATANETSA 253
+ + A + GAG L + S +A SA
Sbjct: 81 LSAVAAPVAFGF--PALSTPGAGGLAV-SISAGALSA 114



Score = 32.4 bits (73), Expect = 0.009
Identities = 19/67 (28%), Positives = 22/67 (32%), Gaps = 9/67 (13%)

Query: 109 GIGGGGGGGDGGDLGGLHGEVGGAGAINLDGTALNVDNTLFVGGAGGGGGSGGAGSTRGG 168
G+G GGG DG + GG + G G GG G G
Sbjct: 26 GLGVGGGASDGSGWSSENNPWGGGSGSGIH---------WGGGSGHGNGGGNGNSGGGSG 76

Query: 169 TGGAGGA 175
TGG A
Sbjct: 77 TGGNLSA 83


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4158PF05616320.008 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 32.0 bits (72), Expect = 0.008
Identities = 16/52 (30%), Positives = 25/52 (48%), Gaps = 2/52 (3%)

Query: 435 AGATGMLS--VGTTCAGAGIIVGVVTLTGLGLKFSGIVIDYAGGSLLLTAIY 484
A + + + + AG++ GV L LG KFS + Y G +LL +Y
Sbjct: 48 ARSLEKVPVKFTASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAHDVY 99


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4167DHBDHDRGNASE792e-19 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 78.6 bits (193), Expect = 2e-19
Identities = 63/280 (22%), Positives = 104/280 (37%), Gaps = 50/280 (17%)

Query: 4 GIRGRHALVFGGSKGMGRACAHQLASEGVNVTIA----------ARTESTLAKAADEITA 53
GI G+ A + G ++G+G A A LAS+G ++ + A+ A+ A
Sbjct: 5 GIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPA 64

Query: 54 --ATGTRVRYVVADITTDEGRGAALSACATPDILVNNADGAPPGDFRQWTQADWHSALDS 111
+ + A I + G DILVN A PG + +W +
Sbjct: 65 DVRDSAAIDEITARIEREMGP---------IDILVNVAGVLRPGLIHSLSDEEWEATFSV 115

Query: 112 MALGPIDMIRRVVDGMMERRFGRIVNIVSRSVKAPQLELGLSNGARSCLVGFVAGLARQT 171
+ G + R V MM+RR G IV + S P+ + +++ V F L +
Sbjct: 116 NSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLEL 175

Query: 172 VRHNVTINNLLPGVFATDAQRHHIEGMLEPGGKTFEQLWTERGANN-------------- 217
+N+ N + PG TD Q LW +
Sbjct: 176 AEYNIRCNIVSPGSTETDMQW---------------SLWADENGAEQVIKGSLETFKTGI 220

Query: 218 PAGRYGEPEELGALCAYICAAQAGYICGQNILIDGAAYPG 257
P + +P ++ ++ + QAG+I N+ +DG A G
Sbjct: 221 PLKKLAKPSDIADAVLFLVSGQAGHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4184HTHTETR952e-26 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 95.1 bits (236), Expect = 2e-26
Identities = 52/209 (24%), Positives = 82/209 (39%), Gaps = 5/209 (2%)

Query: 1 MAGQRKIDALETRERILDAAEWCFCAYGVSHASLEAIAEKASCTRGAIYWHFSGHADLIK 60
MA + K +A ETR+ ILD A F GVS SL IA+ A TRGAIYWHF +DL
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 GIMERGLPPYTKRLEALSYA-PSPLIQKIRECLQECFAAIDGDQHVRNALTILLLRNDFL 119
I E + P + +RE L + ++ R + I+ + +F+
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 120 GLREPFLDSRYQESIEVTAPLALAFRRAISNGEMSSALDPEICAEMINSTMLGILRRSLL 179
G ++ +E + + I + + L A ++ + G++ L
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 180 R-NSCVLAGTGADVLEMAFALIAGISHRP 207
S L D + L+ P
Sbjct: 181 APQSFDLKKEARDYVA---ILLEMYLLCP 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4185HTHTETR598e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.9 bits (142), Expect = 8e-13
Identities = 36/165 (21%), Positives = 55/165 (33%), Gaps = 3/165 (1%)

Query: 14 TPEEILDAAEWCFLHLGVAGTSTALIAARTRCARSLVSAHFPSPRSILQEVLYRGRLPLI 73
T + ILD A F GV+ TS IA R + HF + E+ +
Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71

Query: 74 GHLRRVKATQT-QLIPALRSALQLCLNDILHNERVRATQEILLFHCDLRHLPKDVLEQQI 132
+A + LR L L + ER R EI +FH V++Q
Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEI-IFHKCEFVGEMAVVQQAQ 130

Query: 133 KESAEAM-ALLRSIAVDAKRAGELRENICPESWASILGQLLSGAV 176
+ + A L ++ A I+ +SG +
Sbjct: 131 RNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLM 175


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4188ACRIFLAVINRP11970.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1197 bits (3098), Expect = 0.0
Identities = 605/1032 (58%), Positives = 789/1032 (76%), Gaps = 6/1032 (0%)

Query: 1 MSRFFIDRPIFAWVVAIVIMLAGALSILSLPVNQYPNIAPPAIGIIANYPGASAQTVQDT 60
M+ FFI RPIFAWV+AI++M+AGAL+IL LPV QYP IAPPA+ + ANYPGA AQTVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQQLNGLDGLRYIRSESNADGSVTIVVTFEQGVNPDIAQVQVQNKLSLATPMLPQ 120
VTQVIEQ +NG+D L Y+ S S++ GSVTI +TF+ G +PDIAQVQVQNKL LATP+LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 AVQQLGLRVVKYQVNFMLVAALISEDGRLDNYALADQIVSQLQDPLTRTAGVGDFFVMGS 180
VQQ G+ V K ++++VA +S++ ++D + S ++D L+R GVGD + G+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QNAMRVWLDPLKLNNYALTPGDVIAAIEEQNVQVSSGQLGGRPTAGKVELNATVIGKTLL 240
Q AMR+WLD LN Y LTP DVI ++ QN Q+++GQLGG P +LNA++I +T
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 QTPEQFGAMLLKVNTDGSQVRLRDVADVALGADNFSITTRYNGKPSAGIALRLASGGNTL 300
+ PE+FG + L+VN+DGS VRL+DVA V LG +N+++ R NGKP+AG+ ++LA+G N L
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 EAVKAVQETLSRLEPTLPPGVKVVYPYNTAPVVSESINGVVHTLLEAIVLVFVIMYLFLQ 360
+ KA++ L+ L+P P G+KV+YPY+T P V SI+ VV TL EAI+LVF++MYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NWRATLIPTLAVPVVLLGTFGVMAAVGFTINTLTMFGLVLAIGLLVDDAIVVVENVERLM 420
N RATLIPT+AVPVVLLGTF ++AA G++INTLTMFG+VLAIGLLVDDAIVVVENVER+M
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 VEEGLSPLEATRKSMEQISGALVGIGLVLSAVFIPMAFFGGSTGVIYRQFSLTIVTAMTL 480
+E+ L P EAT KSM QI GALVGI +VLSAVFIPMAFFGGSTG IYRQFS+TIV+AM L
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVFVALIFTPALCATMLKPVHGHH--EKKGFFGWFNRMFERNAQRYESGVTRVVAGRGRY 538
SV VALI TPALCAT+LKPV H K GFFGWFN F+ + Y + V +++ GRY
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 539 MLAFALIVGALAVLFPMMPTSFLPDEDQGTMVVQVELPTNSTADQTDQLLNELSTYLLEE 598
+L +ALIV + VLF +P+SFLP+EDQG + ++LP +T ++T ++L++++ Y L+
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 599 EGDVVDSVFAVNGFSFAGRGQNSGLAFVQLKPWEERKR---SVFDLQASAMQRFSEVKAG 655
E V+SVF VNGFSF+G+ QN+G+AFV LKPWEER S + A +++ G
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 656 TALAFAPPAIQELGNATGFNLFLQDYRGEGHEQLMQVRGQFLAEASKHPA-LTLVRPNGK 714
+ F PAI ELG ATGF+ L D G GH+ L Q R Q L A++HPA L VRPNG
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 715 PDEPQYQVIIDDEKARALGVTLAEVNRTMSTAWGSSYVNDFIDRGRVKRVYVQGIPQARI 774
D Q+++ +D EKA+ALGV+L+++N+T+STA G +YVNDFIDRGRVK++YVQ + R+
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 775 TPEDFNKWYVRNKNGQMVSFASFATGKWVYGSPKLERYNGVPAIEILGEPAPGYSSGDAM 834
PED +K YVR+ NG+MV F++F T WVYGSP+LERYNG+P++EI GE APG SSGDAM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 835 RAVEEIAAKLPSGVSLAWTGLSYEERLSGSQAPALYALSIVAVFLCLAALYESWSIPFSV 894
+E +A+KLP+G+ WTG+SY+ERLSG+QAPAL A+S V VFLCLAALYESWSIP SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 895 LLVVPLGVIGTVAATLMRGLENDAFFQIGLLTTVGLCAKNAILIVEFAKDLHEKGGRTLV 954
+LVVPLG++G + A + +ND +F +GLLTT+GL AKNAILIVEFAKDL EK G+ +V
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 955 QAAIEASRLRLRPIIMTSLAFTMGVIPLAISSGASSGSKHAIGTGVIGGMVTATFLAIFF 1014
+A + A R+RLRPI+MTSLAF +GV+PLAIS+GA SG+++A+G GV+GGMV+AT LAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1015 IPLFYVVVSSLF 1026
+P+F+VV+ F
Sbjct: 1021 VPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4189RTXTOXIND453e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.8 bits (106), Expect = 3e-07
Identities = 20/93 (21%), Positives = 36/93 (38%), Gaps = 2/93 (2%)

Query: 73 EVRPQVTGILLERQFQEGSEVKAGQVLYQINPAPFRATLSRAQASLDSAKLLADRYDRLI 132
E++P I+ E +EG V+ G VL ++ A + Q+SL A+L RY L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 133 ETRAISQQERDDARSQ--YLQARAAVESARIDL 163
+ +++ + + L
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSL 190



Score = 37.1 bits (86), Expect = 9e-05
Identities = 14/86 (16%), Positives = 33/86 (38%), Gaps = 3/86 (3%)

Query: 108 RATLSRAQASLDSAKLLADRYDRLIETRAISQQERDDARSQYLQARAAVESARIDLDFTR 167
++ L + ++ + SAK +L + + + + +
Sbjct: 272 KSQLEQIESEILSAKEEYQLVTQLFKNEILDK--LRQTTDNIGLLTLELAKNEERQQASV 329

Query: 168 ITAPISGRIGRSSV-TQGALVTANQA 192
I AP+S ++ + V T+G +VT +
Sbjct: 330 IRAPVSVKVQQLKVHTEGGVVTTAET 355


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4190HTHTETR566e-12 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.8 bits (134), Expect = 6e-12
Identities = 27/181 (14%), Positives = 58/181 (32%), Gaps = 11/181 (6%)

Query: 13 AKRRKEQVITAAAECVRREGFHRTSMSQISAAAGMSAGHIHHFFGGKDGIIAGIVAREHT 72
A+ ++ ++ A ++G TS+ +I+ AAG++ G I+ F K + + I +
Sbjct: 9 AQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSES 68

Query: 73 ELAQLIEDV--RSSSQGSDAVTAIVKELPRSVPRYMDPGRAALTMEILAEASR-NSEVAH 129
+ +L + + + I+ + S + E + V
Sbjct: 69 NIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQ 128

Query: 130 LIQENDVEVRHAFRDLLGN--------RASDIEARCEIVGALLEGLSARTLRNPQLSTMV 181
+ +E L + I+ + GL L PQ +
Sbjct: 129 AQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLK 188

Query: 182 N 182

Sbjct: 189 K 189


61Bpet4200Bpet4311Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4200225-1.080068IS4 family transposase
Bpetpseudo_15423-0.305112hypothetical protein
Bpet42036221.368068hypothetical protein
Bpet4204519-0.145174hypothetical protein
Bpet42055191.144914hypothetical protein
Bpet42066211.361708amine oxidase, flavin-containing
Bpetpseudo_166190.490373hypothetical protein
Bpetpseudo_176180.562681hypothetical protein
Bpet42075180.378471ABC transporter ATP-binding protein
Bpet42084181.212208iron-hydroxamate transporter permease subunit
Bpet42094190.026221ABC transporter, substrate binding protein
Bpet4210418-0.492397TonB-dependent outer membrane receptor
Bpet4211222-1.167130AraC family transcriptional regulator
Bpet4213427-2.286799single-stranded DNA-binding protein
Bpet4214330-2.856118hypothetical protein
Bpet4215339-5.972258hypothetical protein
Bpet4216442-6.984976hypothetical protein
Bpet4217344-7.107227TetR family transcriptional regulator
Bpet4218243-7.258263hypothetical protein
Bpet4219041-6.462389TetR family transcriptional regulator
Bpet4220039-6.596968putative octaprenyl-diphosphate synthase
Bpet4221140-6.515576MarR family transcriptional regulator
Bpet4222241-6.191768Bcr/CflA family drug resistance transporter
Bpet4223343-6.710368AraC family transcriptional regulator
Bpet4224440-5.399993LysR family transcriptional regulator
Bpet4225440-5.807840DNA-binding
Bpet4226542-5.924981LysR family transcriptional regulator
Bpet4227439-5.055741hypothetical protein
Bpet4228334-4.427340outer membrane efflux protein
Bpet4229223-4.291627putative efflux system transmembrane protein
Bpet4230222-4.053190LysR family transcriptional regulator
Bpet4231023-5.238290transcriptional regulator
Bpet4232119-5.499673LysR family transcriptional regulator
Bpet4233019-6.336032hypothetical protein
Bpet4234020-6.856663DNA-dependent ATPase, SNF2 family protein
Bpet4235129-8.122025hypothetical protein
Bpet4236130-8.514233type III restriction system methylase
Bpet4237225-7.553954type III restriction enzyme
Bpet4238445-9.230423hypothetical protein
Bpet4239447-8.849903hypothetical protein
Bpet4240552-11.331358hypothetical protein
Bpet4241451-13.191946putative transposase
Bpet4242552-13.444107putative transposase
Bpet4243455-13.871378hypothetical protein
Bpet4244451-12.704369hypothetical protein
Bpet4245448-12.188935hypothetical protein
Bpet4246444-10.960338hypothetical protein
Bpet4247545-10.659161hypothetical protein
Bpet4248546-11.277804hypothetical protein
Bpet4249543-10.207737putative protein kinase
Bpet4250543-10.292885hypothetical protein
Bpet4251442-9.660974hypothetical protein
Bpet4252440-9.593767hypothetical protein
Bpet4253025-6.571572hypothetical protein
Bpet4254116-2.488182putative helicase
Bpet4255316-1.501187suppressor protein
Bpet4256316-1.052203hypothetical protein
Bpet4257318-0.482971hypothetical protein
Bpet4258319-0.972578hypothetical protein
Bpet42592190.048145hypothetical protein
Bpet4260215-0.094040hypothetical protein
Bpet4261115-0.251706hypothetical protein
Bpet4262115-0.122704putative secreted protein
Bpet42632150.265751DNA repair protein radC-like protein
Bpet42642150.921593putative secreted protein
Bpet42653160.903665hypothetical protein
Bpet42662171.152651putative lipoprotein
Bpet42672190.980014hypothetical protein
Bpet42683231.246225putative secreted protein
Bpet42693220.015425hypothetical protein
Bpet4270223-0.785953hypothetical protein
Bpet4271324-0.548386hypothetical protein
Bpet42723240.175297hypothetical protein
Bpet42732240.768586hypothetical protein
Bpet42742230.901104hypothetical protein
Bpet42752231.169820hypothetical protein
Bpet42763252.611654hypothetical protein
Bpet4277227-0.638088hypothetical protein
Bpet4278228-2.638210hypothetical protein
Bpet4279326-3.153237hypothetical protein
Bpet4280428-5.257449hypothetical protein
Bpet4281429-5.638766putative lipoprotein
Bpet4282429-6.291175hypothetical protein
Bpet4283526-5.961683hypothetical protein
Bpet4284423-5.031784hypothetical protein
Bpet4285430-7.577372mobile mitochondrial group II intron of COX1
Bpet4286327-6.113593plasmid-related protein
Bpet4287326-5.925888hypothetical protein
Bpet4288324-5.131408hypothetical protein
Bpet4289329-6.771597hypothetical protein
Bpet4290431-7.744175reverse transcriptase
Bpet4291326-5.736907transposase
Bpet4292425-5.193075transposase
Bpet4293425-4.754687hypothetical protein
Bpet4294526-4.891019reverse transcriptase
Bpet4295623-2.342063hypothetical protein
Bpet4296522-2.593656hypothetical protein
Bpet4297522-3.048222hypothetical protein
Bpet4298523-3.225783hypothetical protein
Bpet4299420-4.051775hypothetical protein
Bpet4300419-2.356173hypothetical protein
Bpet4301320-2.110765hypothetical protein
Bpet4302320-1.898662hypothetical protein
Bpet4303318-1.273127hypothetical protein
Bpet4304318-1.629737hypothetical protein
Bpet4305420-1.660392DNA topoisomerase III
Bpet4306322-1.908880single-stranded DNA-binding protein
Bpet4307422-1.647224hypothetical protein
Bpet4308421-1.100540hypothetical protein
Bpet4309422-1.448653hypothetical protein
Bpet4310523-1.458687hypothetical protein
Bpet4311222-2.396987hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4204FLGFLGJ270.045 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 27.0 bits (59), Expect = 0.045
Identities = 14/36 (38%), Positives = 23/36 (63%), Gaps = 1/36 (2%)

Query: 22 STVYAQEVPPPAYQLAAQRAGIPSTVLYAVALQESG 57
S + ++ PA QLA+Q++G+P ++ A A ESG
Sbjct: 149 SKAFLAQLSLPA-QLASQQSGVPHHLILAQAALESG 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4207PF05272357e-04 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 35.4 bits (81), Expect = 7e-04
Identities = 15/48 (31%), Positives = 21/48 (43%), Gaps = 6/48 (12%)

Query: 376 MLFLVGENGSGKTTLIKLLLGLYEPQEGMILLDGAPVVAQNRDDYRQL 423
+ L G G GK+TLI L+GL D + +D Y Q+
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD------FFSDTHFDIGTGKDSYEQI 639


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4209FERRIBNDNGPP1475e-44 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 147 bits (371), Expect = 5e-44
Identities = 88/304 (28%), Positives = 136/304 (44%), Gaps = 15/304 (4%)

Query: 27 VPALERRRFCLGGVAYLAWAGTVPLGALARTDSAQTLGTRGARIACTDWAAAESLALLGC 86
+P + RRR L PL T A + RI +W E L LG
Sbjct: 4 LPLISRRRL-------LTAMALSPLLWQMNTAHAAAIDPN--RIVALEWLPVELLLALGI 54

Query: 87 MPIAVPELAVYRLWLPEPPLPANVADLGSRSEPNLELLAALAPERIVVSSWQAGLLGQFG 146
+P V + YRLW+ EPPLP +V D+G R+EPNLELL + P +V S+
Sbjct: 55 VPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLA 114

Query: 147 RIAPTEVAHIFDGCADPYLRIRELLLQMGTATGLEAQAQMRLREFDTEIECLRAQLAAGA 206
RIAP + DG P R+ L +M L++ A+ L +++ I ++ +
Sbjct: 115 RIAPGRGFNFSDG-KQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFV--K 171

Query: 207 DAARSVYMAVLHENGAQAFVYGQGSWVNRVLGQLGLRNAWSARTTFYGNSLVGIAALAAE 266
AR + + L + V+G S +L + G+ NAW T F+G++ V I LAA
Sbjct: 172 RGARPLLLTTLID-PRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAY 230

Query: 267 PEAVILYLDQGARTRRAEALLRDSTLWRSLPAVASGRAHAIASFYALGGLASAQRCARLV 326
+ +L D L + LW+++P V +GR + + + G SA R++
Sbjct: 231 KDVDVLCFDHDNSKDMDA--LMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVL 288

Query: 327 VGAL 330
A+
Sbjct: 289 DNAI 292


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4214ARGREPRESSOR320.003 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 31.8 bits (72), Expect = 0.003
Identities = 15/46 (32%), Positives = 20/46 (43%), Gaps = 12/46 (26%)

Query: 168 SQSELARSLAADGFPVQQSHISRMAD---AVR---------YLLPA 201
+Q EL L DG+ V Q+ +SR V+ Y LPA
Sbjct: 21 TQDELVDILKKDGYNVTQATVSRDIKELHLVKVPTNNGSYKYSLPA 66


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4217HTHTETR565e-12 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 56.2 bits (135), Expect = 5e-12
Identities = 28/144 (19%), Positives = 53/144 (36%), Gaps = 11/144 (7%)

Query: 22 HTVLQAARKVFLTHGFSATT-DMIQQMAGVSKSTVYAHYANKETLFSAVIEAECQSFSDK 80
+L A ++F G S+T+ I + AGV++ +Y H+ +K LFS + E S S+
Sbjct: 14 QHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE---LSESNI 70

Query: 81 VSAIRFQSGKLKDTLAALGSAYLDIVLT-----PEKLALYRIVIAEAPRFPRLA--HKFY 133
K ++ L VL + L I+ + +A +
Sbjct: 71 GELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQ 130

Query: 134 EAGPNAVVSIVARYLDIAVTSREL 157
+ + L + ++ L
Sbjct: 131 RNLCLESYDRIEQTLKHCIEAKML 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4219HTHTETR614e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 61.2 bits (148), Expect = 4e-14
Identities = 27/128 (21%), Positives = 46/128 (35%), Gaps = 7/128 (5%)

Query: 2 RRAAIQQAALDVFSECGYTRATMREIARRAGVTHGLVQRHFGSKEALFLATVPGT----R 57
R I AL +FS+ G + ++ EIA+ AGVT G + HF K LF +
Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71

Query: 58 DWESVIIQEGKGSLAERIAAAFTDRGEA---GTGLDALVALLRSTASDIGAAKKLYGVMR 114
+ E + G + E+ L+ ++ +G + R
Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQR 131

Query: 115 DGGAALYE 122
+ Y+
Sbjct: 132 NLCLESYD 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4222TCRTETB545e-10 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 53.7 bits (129), Expect = 5e-10
Identities = 39/185 (21%), Positives = 71/185 (38%), Gaps = 6/185 (3%)

Query: 18 RLLATLSLLLAFGNASVELYLPGLPSMAAALHASPAEAQWTLSSFLVGFGIGQLFWGPVG 77
++L L +L F + + LP +A + PA W ++F++ F IG +G +
Sbjct: 14 QILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLS 73

Query: 78 DSFGRRIPLLVAISVYIVASLGCILSSSIHEV-IAWRLLQAFGACAGPVLARAVVRDVYG 136
D G + LL I + S+ + S + I R +Q GA A P L VV
Sbjct: 74 DQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIP 133

Query: 137 HHRSADILSLLLLAASFAPLLLPLAGGGLL-WFGWRAIFWGQVAFGAVAMVGMLLMDETL 195
L+ + + P GG + + W + + ++ + + + L
Sbjct: 134 KENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL----LIPMITIITVPFLMKLL 189

Query: 196 PQARR 200
+ R
Sbjct: 190 KKEVR 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4229ACRIFLAVINRP2268e-71 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 226 bits (577), Expect = 8e-71
Identities = 114/185 (61%), Positives = 149/185 (80%), Gaps = 1/185 (0%)

Query: 10 IAATLPTGIGYEWTGLSFQEKVAGSQALGLFALAILVVFLLLVALYESWSIPLSVMLIVP 69
+A+ LP GIGY+WTG+S+QE+++G+QA L A++ +VVFL L ALYESWSIP+SVML+VP
Sbjct: 846 LASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVP 905

Query: 70 IGAIGAVLAVTVVGMPNDVYFKVGLITVIGLAAKNAILIVEFAKDL-RSEGHTAMEAAVT 128
+G +G +LA T+ NDVYF VGL+T IGL+AKNAILIVEFAKDL EG +EA +
Sbjct: 906 LGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLM 965

Query: 129 AAKMRFRPILMTSLAFILGVVPLAIAAGAGAASQRALGTGVIGGMLAATTLGVIFVPVFY 188
A +MR RPILMTSLAFILGV+PLAI+ GAG+ +Q A+G GV+GGM++AT L + FVPVF+
Sbjct: 966 AVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFF 1025

Query: 189 VWVMK 193
V + +
Sbjct: 1026 VVIRR 1030



Score = 59.1 bits (143), Expect = 2e-12
Identities = 31/156 (19%), Positives = 65/156 (41%), Gaps = 1/156 (0%)

Query: 43 AILVVFLLLVALYESWSIPLSVMLIVPIGAIGAVLAVTVVGMPNDVYFKVGLITVIGLAA 102
AI++VFL++ ++ L + VP+ +G + G + G++ IGL
Sbjct: 347 AIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLV 406

Query: 103 KNAILIVEFAKDLRSEGHTA-MEAAVTAAKMRFRPILMTSLAFILGVVPLAIAAGAGAAS 161
+AI++VE + + E EA + ++ ++ +P+A G+ A
Sbjct: 407 DDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAI 466

Query: 162 QRALGTGVIGGMLAATTLGVIFVPVFYVWVMKQVAR 197
R ++ M + + +I P ++K V+
Sbjct: 467 YRQFSITIVSAMALSVLVALILTPALCATLLKPVSA 502


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4240BACINVASINB300.018 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 29.7 bits (66), Expect = 0.018
Identities = 37/160 (23%), Positives = 63/160 (39%), Gaps = 31/160 (19%)

Query: 21 SVREALEAACREQIAVAEQKCAEAREEAQNSASMLESSIQQEQAATQNVDGAEQALDGSQ 80
+V +A+ + +E ++ A EAQ + + E+SI++ A D A + L +Q
Sbjct: 109 AVWQAMIESQKEMGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAATKKLTQAQ 168

Query: 81 SSLSSAESALSACLSQPHDDDGRCPDCSGEDSAVAEAEAAVEQAQSMLEQARAELDVATE 140
+ L S + A D A+AEAAVEQA ATE
Sbjct: 169 NKLQSLDPA---------------------DPGYAQAEAAVEQAG----------KEATE 197

Query: 141 DRISMEQRVDLAKQAQAMAEHTLEQTLQACNAHLATVDQA 180
+ ++++ D +A A+ E+ T + A
Sbjct: 198 AKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAA 237


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4252GPOSANCHOR459e-07 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 45.1 bits (106), Expect = 9e-07
Identities = 59/331 (17%), Positives = 110/331 (33%), Gaps = 21/331 (6%)

Query: 46 TAERQVIEQDKAKLAQREQAVTQAEQKCDAGFADERAALNDELREKRAQGERAIAEMREK 105
A + +E+ A + + +A A A +L + K
Sbjct: 119 EARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAAR-KADLEKALEGAMNFSTADSAK 177

Query: 106 NLSALEVEISELKAKRLGAVAHAENAERERIRTEIAQERDAWTKQQ-GDARKQLNAERTE 164
LE E + L+A++ E A + K + L
Sbjct: 178 I-KTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEG 236

Query: 165 FEKQKGALSALQSEVEGRQAELETSERTLERKEQ---------RLEQQNQRRSEQLDDEV 215
A SA +E +A LE + LE+ + + + + +
Sbjct: 237 AMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAE 296

Query: 216 ERRVEDRRKSLEAALQSAKEENIRLREAFKTQDELLGAFEQLKLQLGGKDPAEILRALNS 275
+ +E + + L A QS + + REA K + E+ ++ + R L++
Sbjct: 297 KADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQN-KISEASRQSLRRDLDA 355

Query: 276 QADELKRLREELATRPTEEMRERYQALESEAKNQKTRADQLERQLSTNEAAVAEIGELRR 335
+ K+L E ++ E+ + SEA Q R D L+ + + E
Sbjct: 356 SREAKKQLEAEHQ-----KLEEQNKI--SEASRQSLRRD-LDASREAKKQVEKALEEANS 407

Query: 336 QGSELNAENKSLAQRASIFEGAANEAQAELK 366
+ + L NK L + + E E QA+L+
Sbjct: 408 KLAALEKLNKELEESKKLTEKEKAELQAKLE 438



Score = 38.1 bits (88), Expect = 2e-04
Identities = 78/338 (23%), Positives = 142/338 (42%), Gaps = 35/338 (10%)

Query: 14 LADLNARESWINTKESEIASRETAVATRERDATAERQVIEQDKAKLAQREQAVTQAEQKC 73
A L AR++ + + TA + + + AE+ + KA L + +
Sbjct: 185 KAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEG-AMNFSTA 243

Query: 74 DAGFADERAALNDELREKRAQGERAIAEMREK------NLSALEVEISELKAK--RLGAV 125
D+ A L ++A+ E+A+ + LE E + L+A+ L
Sbjct: 244 DSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQ 303

Query: 126 AHAENAERERIRTEIAQERDAWTKQQGDARKQLNAERTEFEKQKGALSALQSEVEGRQAE 185
+ NA R+ +R ++ R +A+KQL AE + E+Q A + +
Sbjct: 304 SQVLNANRQSLRRDLDASR--------EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDA 355

Query: 186 LETSERTLERKEQRLEQQNQ---RRSEQLDDEVERRVEDRRKSLEAALQSAKEENIRLRE 242
+++ LE + Q+LE+QN+ + L +++ + +K +E AL+ A + L +
Sbjct: 356 SREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDAS-REAKKQVEKALEEANSKLAALEK 414

Query: 243 AFKTQDELLGAFEQLKLQLGGKDPAEILR---ALNSQADELKRLREELATRP-------- 291
K +E E+ K +L K AE L QA+EL +LR A+
Sbjct: 415 LNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQAEELAKLRAGKASDSQTPDAKPG 474

Query: 292 TEEMRERYQALESEAKNQKTRAD--QLERQL-STNEAA 326
+ + + QA ++ K + +A + +RQL ST E A
Sbjct: 475 NKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGETA 512


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4257OMPADOMAIN290.004 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 28.7 bits (64), Expect = 0.004
Identities = 12/28 (42%), Positives = 16/28 (57%), Gaps = 5/28 (17%)

Query: 19 RGWRAY--ARGERRAS---NWLVSKGVP 41
G AY ERRA ++L+SKG+P
Sbjct: 264 IGSDAYNQGLSERRAQSVVDYLISKGIP 291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4266VACJLIPOPROT270.038 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 26.8 bits (59), Expect = 0.038
Identities = 11/25 (44%), Positives = 12/25 (48%)

Query: 13 ALALAVALLGGCATSKEKLLTHGDS 37
ALAL LL GCA+S D
Sbjct: 7 ALALGTTLLVGCASSGTDQQGRSDP 31


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4269PF04335300.006 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 30.2 bits (68), Expect = 0.006
Identities = 17/113 (15%), Positives = 34/113 (30%), Gaps = 14/113 (12%)

Query: 108 LKADYDYRR--STGELRQRVRGIYEI-----PGRSYGDNPTARVRVIS---DRDWVVTLD 157
+ +D S + R Y+ P + V + V +
Sbjct: 114 REEYFDAVMVMSARPEQDRWSRFYKTDNPQSPQNILANRTDVFVEIKRVSFLGGNVAQVY 173

Query: 158 ISADEYYGAEQV---KRALVRYPVK-VARVDVDPARNPFGLVIDCYEGTPQRI 206
+ + G+ A ++Y V +VD +NP G ++ Y +
Sbjct: 174 FTKESVTGSNSTKTDAVATIKYKVDGTPSKEVDRFKNPLGYQVESYRADVEVP 226


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4278FLGFLGJ270.050 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 27.0 bits (59), Expect = 0.050
Identities = 14/30 (46%), Positives = 21/30 (70%), Gaps = 1/30 (3%)

Query: 33 ELPPPAYQLAAQRAGIPSTVLYAVALQESG 62
+L PA QLA+Q++G+P ++ A A ESG
Sbjct: 155 QLSLPA-QLASQQSGVPHHLILAQAALESG 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4280ICENUCLEATIN310.004 Ice nucleation protein signature.
		>ICENUCLEATIN#Ice nucleation protein signature.

Length = 1258

Score = 30.9 bits (69), Expect = 0.004
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 7/58 (12%)

Query: 111 RAEVEQIKARPSALR--AAAPAQPRSPSRPTAKPEPPPLPFRIVGAELRAGQRSLSVT 166
RAEV + + SA++ A + + A P P + +E++ G RSL VT
Sbjct: 89 RAEVLHVGTKTSAMQFILHHRADYVACTEMQAGPGSPDVT-----SEVKVGNRSLPVT 141


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4291HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4311ARGREPRESSOR320.002 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 32.1 bits (73), Expect = 0.002
Identities = 16/46 (34%), Positives = 20/46 (43%), Gaps = 12/46 (26%)

Query: 168 SQSELARRLAADGYPVQQSHISRMAD---AVR---------YLLPA 201
+Q EL L DGY V Q+ +SR V+ Y LPA
Sbjct: 21 TQDELVDILKKDGYNVTQATVSRDIKELHLVKVPTNNGSYKYSLPA 66


62Bpet4322Bpetpseudo_18Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet43222140.444159prolyl iminopeptidase
Bpet43232150.785591thiamin biosynthesis ThiS
Bpet43243141.035115putative secreted protein
Bpet43252140.179175LysM domain/BON superfamily protein
Bpet43262130.849808hypothetical protein
Bpet43272141.519906hypothetical protein
Bpet43281160.954060hypothetical protein
Bpet43292170.600853N-acetyl-anhydromuranmyl-L-alanine amidase
Bpet4330115-0.190523glutathione S-transferase
Bpet4331118-2.003752MarR family transcriptional regulator
Bpet4332014-1.503591hypothetical protein
Bpet4333211-2.922542hypothetical protein
Bpetpseudo_18213-3.146097hypothetical protein
63Bpet4356Bpet4365Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet43562111.582190hypothetical protein
Bpet43572120.614875high-affinity nitrate transporter, putative
Bpet4358212-0.103904ribose ABC transporter ATP-binding protein
Bpet4359111-1.396946ribose ABC transporter substrate-binding
Bpet4360012-2.307955ribose ABC transporter permease
Bpet4361012-2.764778putative carbohydrate kinase
Bpet4362-212-4.113611hypothetical protein
Bpet4363-110-3.190891putative short chain dehydrogenase
Bpet4364-210-3.202330putative TrapT dctQ-M fusion permease,
Bpet4365-112-3.026180C4-dicarboxylate periplasmic binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4357TCRTETB290.028 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.5 bits (66), Expect = 0.028
Identities = 34/199 (17%), Positives = 74/199 (37%), Gaps = 14/199 (7%)

Query: 8 IDLFTFNTVPMRAFHLSWMAFFVCFFAWFASAPLMPVIAREFALTPGQVADINI-AAVAA 66
+D +P L F + + P M + L+ ++ + I +
Sbjct: 248 VDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYM--MKDVHQLSTAEIGSVIIFPGTMS 305

Query: 67 TILVRLIVGPLCDRYGPRRVYAGLMASGAIPVVALAFATDYAS---VLACRLGIGAIGAS 123
I+ I G L DR GP V + ++ + +F + S + +G + +
Sbjct: 306 VIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFT 365

Query: 124 FVITQYHTSVMFAPNVVGAANAA-------SAGWGNAGAGGAQALVPMVLAALIALGLDE 176
+ S GA + S G G A GG ++ P++ L+ + +D+
Sbjct: 366 KTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSI-PLLDQRLLPMEVDQ 424

Query: 177 SSAWRAALVVPGAALLAMA 195
S+ + L++ + ++ ++
Sbjct: 425 STYLYSNLLLLFSGIIVIS 443


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4363DHBDHDRGNASE1221e-35 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 122 bits (306), Expect = 1e-35
Identities = 81/268 (30%), Positives = 122/268 (45%), Gaps = 15/268 (5%)

Query: 6 MEHKQFSGRVALVLGAGSVGEGWGNGKAAAVAYAREGATVIAVDLNLDAARETHGIIHQE 65
M K G++A + GA G G+A A A +GA + AVD N + + + E
Sbjct: 1 MNAKGIEGKIAFITGAAQ-----GIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE 55

Query: 66 GGRSEALAADVTQADQVAALVQGVVERHGRIDILHNNVGMARMGSVTELSEAQWDTAMNV 125
+EA ADV + + + + G IDIL N G+ R G + LS+ +W+ +V
Sbjct: 56 ARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSV 115

Query: 126 NLKSAFLACKHVLPVMQAQKRGSIVNISSLAAIRYTGYP---YPVYYASKGGLNQLTVGL 182
N F A + V M ++ GSIV + S A G P Y +SK T L
Sbjct: 116 NSTGVFNASRSVSKYMMDRRSGSIVTVGSNPA----GVPRTSMAAYASSKAAAVMFTKCL 171

Query: 183 ALEYAKQGIRVNAIMPGYVDTPLIYKDISGQYGSREEM---VNERNARCPMGHMGTAWDI 239
LE A+ IR N + PG +T + + + + G+ + + + P+ + DI
Sbjct: 172 GLELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDI 231

Query: 240 ANAAVFLASDAAAYITGVCLPVDGGVHL 267
A+A +FL S A +IT L VDGG L
Sbjct: 232 ADAVLFLVSGQAGHITMHNLCVDGGATL 259


64Bpet4393Bpet4413Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet43931183.461275hypothetical protein
Bpet43943193.917031bacteriophage protein
Bpet43955193.997107hypothetical protein
Bpet43966184.374646hypothetical protein
Bpet43976164.164890hypothetical protein
Bpet43986183.356848hypothetical protein
Bpet43996192.371809putative phage-related membrane protein
Bpet44005202.571947phage-related lytic murein transglycosylase
Bpet44015221.413092hypothetical protein
Bpet44021171.140510putative lipoprotein
Bpet44030171.390439hypothetical protein
Bpet44040181.880871hypothetical protein
Bpet44051201.992153putative bacteriophage protein GP26
Bpet44061191.688686putative bacteriophage protein
Bpet44071191.790138Mu-like prophage FluMu protein gp28
Bpet44082201.609424hypothetical protein
Bpet44093201.494760F protein (gpF) (protein gp30)
Bpet44103211.155567putative bacteriophage protein
Bpet44112191.456893hypothetical protein
Bpet44122211.928032putative lipoprotein
Bpet44132191.145111hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4402VACJLIPOPROT280.007 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 27.9 bits (62), Expect = 0.007
Identities = 12/31 (38%), Positives = 14/31 (45%)

Query: 1 MIARLMVLACVTAALAGCAGAPASPPPPTDP 31
M RL LA T L GCA + +DP
Sbjct: 1 MKLRLSALALGTTLLVGCASSGTDQQGRSDP 31


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4404FbpA_PF05833300.001 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 30.2 bits (68), Expect = 0.001
Identities = 6/46 (13%), Positives = 22/46 (47%), Gaps = 3/46 (6%)

Query: 40 AIDQVDDRVTKTQQR---LDRVEQTLENRPGYADLHAVRAEMAQTN 82
+ + ++++ + ++ L V + N Y ++ ++ E+ +T
Sbjct: 396 SEEAANEQLLQNEEELNYLYSVLTNINNADNYDEIEEIKKELIETG 441


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4408OMADHESIN290.037 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

Length = 455

Score = 29.5 bits (65), Expect = 0.037
Identities = 27/90 (30%), Positives = 37/90 (41%), Gaps = 12/90 (13%)

Query: 401 APFGFAERDDGSVRLRADTMADRLDREAEPAMAALMEPVRRLVANAGSLQEIRDGLFSLY 460
+P+ FA+ DG L A ++ D PA+ L PVR V AG L G+ S+
Sbjct: 20 SPYAFADDYDGIPNLTAVQISPNAD----PAL-GLEYPVRPPVPGAGGLNASAKGIHSIA 74

Query: 461 EGMPSEQLAVVMRRAMAAAALAGRADVVEG 490
G +E A AA G + G
Sbjct: 75 IGATAEA-------AKGAAVAVGAGSIATG 97


65Bpet4462Bpet4468Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4462216-1.100327LysR family transcriptional regulator
Bpet4463418-3.025171hypothetical protein
Bpet4464323-3.970227type-1 fimbrial protein
Bpet4465323-3.633243type-1 fimbrial protein
Bpet4466222-3.960217pili assembly chaperone
Bpet4467021-3.204633outer membrane usher protein
Bpet4468-124-3.313137putative fimbrial adhesin
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4467PF005777700.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 770 bits (1989), Expect = 0.0
Identities = 282/873 (32%), Positives = 420/873 (48%), Gaps = 40/873 (4%)

Query: 11 LRYACGLVSALFACGIGASVAAEASSAQVAEVQFNTDMLRGFGDAPVDISRFNRGNFAAP 70
L ++ F A A + AE+ FN L A D+SRF G P
Sbjct: 16 LHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPP 75

Query: 71 GDYTVPIVVNDRRVGRGTVRLRQLAGEAYPQPCVDTDLLTTAGVNVQRLDDAAQAQLQEN 130
G Y V I +N+ + V E PC+ L + G+N + ++
Sbjct: 76 GTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLA--DD 133

Query: 131 SCVRLPGLIPDARAEFDNGEQHLYLSIPQIWLNRSARGYVDPDHWNEGITAGMLRYNANV 190
+CV L +I DA A+ D G+Q L L+IPQ +++ ARGY+ P+ W+ GI AG+L YN +
Sbjct: 134 ACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSG 193

Query: 191 YRYNSRHGSASTQGYLGLDSGFNVGAWRFRHRGNLSYQENLG-----THYESIQTSVQRS 245
+R G S YL L SG N+GAWR R SY + ++ I T ++R
Sbjct: 194 NSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERD 253

Query: 246 LAPIKSQLTAGEFFTEGDVLESLNLRGVRLSSDDRMYPESLRGYAPTVHGIANSNARVSI 305
+ P++S+LT G+ +T+GD+ + +N RG +L+SDD M P+S RG+AP +HGIA A+V+I
Sbjct: 254 IIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTI 313

Query: 306 RQNGIVIYETTVAPGEFQIDDLYPTGYGGDLEVVVTEADGSVHISRVPFSAPINALRAGA 365
+QNG IY +TV PG F I+D+Y G GDL+V + EADGS I VP+S+ R G
Sbjct: 314 KQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGH 373

Query: 366 TRYSLAAGQYRNTMG-GETPYVFQGTVRHGFNNLVTGYGGITASEHYLAGEVGAALNT-S 423
TRYS+ AG+YR+ E P FQ T+ HG T YGG ++ Y A G N +
Sbjct: 374 TRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGA 433

Query: 424 WGAFSLDATYARARLRNQPDRRGQSYGLSYSRLYEPTATSVTLAAYRYSTDGFLNLADTV 483
GA S+D T A + L + GQS Y++ + T++ L YRYST G+ N ADT
Sbjct: 434 LGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTT 493

Query: 484 ALRSAD--------------SLYALPRGYGSAKGRLQVMLNQPLGERWGSLYLSGYSQNY 529
R + +G+LQ+ + Q LG R +LYLSG Q Y
Sbjct: 494 YSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLG-RTSTLYLSGSHQTY 552

Query: 530 WGHSGRDTEYQAGYSNSFKRVNYNISASRQYSAYSGKWENTYMLNFSLPLGSGANAPR-- 587
WG S D ++QAG + +F+ +N+ +S S +A+ + LN ++P +
Sbjct: 553 WGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKS 612

Query: 588 ------SNTTIQRNNRTRSTFIYETVNGSLGGSDSPLYYGVSASHSRHGGQGANSNNVSA 641
++ ++ + R T V G+L D+ L Y V ++ GG G + + A
Sbjct: 613 QWRHASASYSMSHDLNGRMT-NLAGVYGTL-LEDNNLSYSVQTGYAG-GGDGNSGSTGYA 669

Query: 642 NASWTSPLAQLGASASRSSNSSQASASISGAAVAWGGGVALTPSLGDTFAIVDAQGAAGA 701
++ S S + Q +SG +A GV L L DT +V A GA A
Sbjct: 670 TLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDA 729

Query: 702 RIANMGGLRVNSRGYGVVSNLTPFAQNTIEVDPNGLPLNVQFKSTIQHVAPTAGAIVPVK 761
++ N G+R + RGY V+ T + +N + +D N L NV + + +V PT GAIV +
Sbjct: 730 KVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAE 789

Query: 762 FEVEAGGQAAVIRARQADGQALPFGAQALDGNGNQVGTVAQGSRIIASSLKDTKGRITIK 821
F+ G ++ + + LPFGA + G VA ++ S + G++ +K
Sbjct: 790 FKARVG--IKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPL-AGKVQVK 846

Query: 822 WGATAAQQCTVDYALPEAAGKADQPFHLLQGTC 854
WG C +Y LP Q L C
Sbjct: 847 WGEEENAHCVANYQLPPE--SQQQLLTQLSAEC 877


66Bpet4480Bpet4519Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet44802172.112069LysR family transcriptional regulator
Bpet44811150.453789putative hemin permease
Bpet4482013-0.646933hemin importer ATP-binding subunit
Bpet4483-112-1.040640mandelate racemase/muconate lactonizing protein
Bpet4484013-0.854964LysR family transcriptional regulator
Bpet4485-111-2.044428ABC transporter, ATP-binding protein
Bpet4486012-2.918283hypothetical protein
Bpet4487012-2.517385lysine-specific permease
Bpet4488013-3.183808putative dehydrogenase
Bpet4489015-3.852021LysR family transcriptional regulator
Bpet4490222-5.947982ABC transporter substrate-binding protein
Bpet4491326-6.584453putative branched-chain amino acid transport
Bpet4492530-7.146094putative branched-chain amino acid transport
Bpet4493535-8.530202putative branched-chain amino acid ABC
Bpet4494739-8.804642putative branched-chain amino acid ABC
Bpet4495842-9.464886hypothetical protein
Bpet4496640-10.124700hypothetical protein
Bpet4497643-10.381427hypothetical protein
Bpet4498445-10.203763hypothetical protein
Bpet4499249-8.818765hypothetical protein
Bpet4500137-6.754748hypothetical protein
Bpet4501023-4.106921hypothetical protein
Bpet4502015-2.692771hypothetical protein
Bpet4503211-0.666669hypothetical protein
Bpet4504-115-0.048694hypothetical protein
Bpet4505-1201.259574hypothetical protein
Bpet4506-1181.688697hypothetical protein
Bpet4507-1201.833776LysR family transcriptional regulator
Bpet4508-1222.225822HlyD family secretion protein
Bpet4509-2211.987522AcrB/AcrD/AcrF family protein
Bpet45101183.933912putative outer membrane efflux protein
Bpet45110173.171891putative 2'-5' RNA ligase
Bpet4512-1172.489483putative 5'(3')-deoxyribonucleotidase
Bpet4513-1172.012307hypothetical protein
Bpet45141152.391363LysR family transcriptional regulator
Bpet45151152.685074putative transmembrane efflux protein of the MFS
Bpet45161142.776667oxidoreductase
Bpet45172153.152633putative short chain dehydrogenase
Bpet4518-1153.017696dihydroxy-acid dehydratase
Bpet45190143.417110LysR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4482PF05272300.010 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.010
Identities = 14/37 (37%), Positives = 18/37 (48%), Gaps = 4/37 (10%)

Query: 19 SGVSLAVEPG----QVLGLLGANGAGKSTLLAALAGE 51
V+ +EPG + L G G GKSTL+ L G
Sbjct: 583 GHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4499TYPE4SSCAGA290.006 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 29.3 bits (65), Expect = 0.006
Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 8/75 (10%)

Query: 34 VQIEQVTKERAKWRDS-IRVFAEATATAWEEHQVAPNPAKTAALRARLATSINP--KDDE 90
+ +E TK K+ D R+F T+W HQ P+ T ++R + I P DD+
Sbjct: 100 IDVESSTKSFQKFGDQRYRIF-----TSWVSHQNDPSKINTRSIRNFMENIIQPPILDDK 154

Query: 91 QDAKILSHFDDLFSG 105
+ A+ L F+G
Sbjct: 155 EKAEFLKSAKQSFAG 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4506PF04183290.032 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 29.1 bits (65), Expect = 0.032
Identities = 40/176 (22%), Positives = 58/176 (32%), Gaps = 24/176 (13%)

Query: 123 GMAVPDWISSLPAVGPRLAVYWQTYLGEPHALGALVELVSG---------EHLGNIYRMV 173
G W+ + A L LGEP A E + E LG I+R
Sbjct: 295 GPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRE- 353

Query: 174 LSATGNAFQLLLN--VVFMLITLFFVYKDGDRMIAQLDVLGERILPTRWQR--FSRVVPA 229
N + L ++ TL ++ + + W F VV
Sbjct: 354 -----NPCRWLKPDESPVLMATLMECDENNQPLAGAYIDR-SGLDAETWLTQLFRVVVVP 407

Query: 230 TVGS-TVTGMSLIAVGEGVVLGVAYWLAGVPSPVLLGVVTGFMALIPGGAPLSFTL 284
G++LIA G+ + L + GVP VLL G M L+ P +L
Sbjct: 408 LYHLLCRYGVALIAHGQNITLAMK---EGVPQRVLLKDFQGDMRLVKEEFPEMDSL 460


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4508RTXTOXIND522e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.8 bits (124), Expect = 2e-09
Identities = 18/98 (18%), Positives = 36/98 (36%), Gaps = 5/98 (5%)

Query: 110 AEVDRAAAQLAAARARVAFTASELARGK-RLLAENAIARRDFESKRNDAREAAANLQAAE 168
+ A +L ++++ SE+ K + + + K + N+
Sbjct: 259 NKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTD---NIGLLT 315

Query: 169 AALDAAKLNLGYTEIVAPVDGRVSRAEI-TEGNVVAAG 205
L + + I APV +V + ++ TEG VV
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353



Score = 50.2 bits (120), Expect = 8e-09
Identities = 26/148 (17%), Positives = 47/148 (31%), Gaps = 31/148 (20%)

Query: 49 VAPALGKTIVDWQDYSGRLEAIDRVDIRPLVSGTLTAVHFQDGSLVHKGDPLFTIDPRPY 108
VA A GK SGR +I+P+ + + + ++G V KGD L +
Sbjct: 83 VATANGKLTH-----SGR-----SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGA 132

Query: 109 AAEVDRAAAQLAAARARVA---------------------FTASELARGKRLLAENAIAR 147
A+ + + L AR + + +L ++ +
Sbjct: 133 EADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIK 192

Query: 148 RDFESKRNDAREAAANLQAAEAALDAAK 175
F + +N + NL A
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVL 220


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4509ACRIFLAVINRP10980.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1098 bits (2841), Expect = 0.0
Identities = 436/1042 (41%), Positives = 663/1042 (63%), Gaps = 16/1042 (1%)

Query: 3 ISKFFIDRPIFAGVLSVIVLLAGLLAMFQLPISEYPEVVPPSVVVRAQYPGANPKVIAAT 62
++ FFI RPIFA VL++I+++AG LA+ QLP+++YP + PP+V V A YPGA+ + + T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VASPLEESINGVEDMLYMQSQANSDGNLAVTVYFKLGVDPDKAQQLVQNRVSQALPRLPP 122
V +E+++NG+++++YM S ++S G++ +T+ F+ G DPD AQ VQN++ A P LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 123 DVQRLGVTTTKSSPTLTLVVHLISPNDRYDITYLRNYAVLNVKDRLSRIGGVGEVQIWGS 182
+VQ+ G++ KSS + +V +S N + +Y NVKD LSR+ GVG+VQ++G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 183 GSYSMRVWLDPQKVAQRGLTATDVVNAIREQNVQVAAGVIGASPTQGDVPMQFSVNAQGR 242
Y+MR+WLD + + LT DV+N ++ QN Q+AAG +G +P + S+ AQ R
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 243 LQNETEFGNIILKSSPDGAVTRLSDVARIELGAQEYGLRSLLNNKPAIGMGIMQSPGANA 302
+N EFG + L+ + DG+V RL DVAR+ELG + Y + + +N KPA G+GI + GANA
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 303 LDVSAQVRETMKELSADFPPGLEYRIEYDPTQFVRSSIKAVISTLLEAIALVVLVVIVFL 362
LD + ++ + EL FP G++ YD T FV+ SI V+ TL EAI LV LV+ +FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 363 QTWRASIIPLLAVPVSIVGTFSLLLLFGYSINALSLFGMVLAIGIVVDDAIVVVENVERN 422
Q RA++IP +AVPV ++GTF++L FGYSIN L++FGMVLAIG++VDDAIVVVENVER
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 423 IA-AGLTPREATYRAMREVSGPIIAIALTLCAVFVPLAFMTGLSGQFYKQFAMTIAISTV 481
+ L P+EAT ++M ++ G ++ IA+ L AVF+P+AF G +G Y+QF++TI +
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 482 ISAFNSLTLSPALSALLLKGHHDKPDWLTRGMNRVFGGFFNWFNRFFGRASDSYATGITG 541
+S +L L+PAL A LLK ++ + GGFF WFN F + + Y +
Sbjct: 480 LSVLVALILTPALCATLLKP-------VSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGK 532

Query: 542 VIRRKGGAMAVYAVLLAATVGISYLVPGGFVPAQDKQYLIGFAQLPNGASLDRTEDVIRR 601
++ G + +YA+++A V + +P F+P +D+ + QLP GA+ +RT+ V+ +
Sbjct: 533 ILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQ 592

Query: 602 MSDIALK--EPGVESAIAFPGLSINGFTNSSSAGIVFVTLKPFDERHSAELSGNAITGSL 659
++D LK + VES G S +G + +AG+ FV+LKP++ER+ E S A+
Sbjct: 593 VTDYYLKNEKANVESVFTVNGFSFSG--QAQNAGMAFVSLKPWEERNGDENSAEAVIHRA 650

Query: 660 NAKFASIKDAFIAVFPPPPVMGLGTMGGFKLQIEDRAALGYAELDKATQAFLAKARQAP- 718
+ I+D F+ F P ++ LGT GF ++ D+A LG+ L +A L A Q P
Sbjct: 651 KMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPA 710

Query: 719 ELGPTFSNYQINVPQLDVDLDRVKAKQLGVPVTDVFDTLQIYLGSMYVNDFNRFGRVFQV 778
L N + Q +++D+ KA+ LGV ++D+ T+ LG YVNDF GRV ++
Sbjct: 711 SLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKL 770

Query: 779 RAQADAPFRAHPDDILQLKTRSDSGQMVPLSALVDVKQTFGPEMVVRYNGYTAADINGGP 838
QADA FR P+D+ +L RS +G+MVP SA +G + RYNG + +I G
Sbjct: 771 YVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA 830

Query: 839 APGYSSDQAQDAAERIAAETLPRGVKFEWTDLTYQQILAGNAGIWVFPISVLLVFLVLAA 898
APG SS A E +A++ LP G+ ++WT ++YQ+ L+GN + IS ++VFL LAA
Sbjct: 831 APGTSSGDAMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAA 889

Query: 899 LYESLTLPLAVILIVPMSILAALTGVWLTSGDNNIFTQIGLMVLVGLSAKNAILIVEFAR 958
LYES ++P++V+L+VP+ I+ L L + N+++ +GL+ +GLSAKNAILIVEFA+
Sbjct: 890 LYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAK 949

Query: 959 EL-EMQGSTPLQAAIEASRLRLRPILMTSIAFIMGVVPLVLSSGAGSEMRHAMGVAVFFG 1017
+L E +G ++A + A R+RLRPILMTS+AFI+GV+PL +S+GAGS ++A+G+ V G
Sbjct: 950 DLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGG 1009

Query: 1018 MLGVTLFGLFLTPVFYVLLRTL 1039
M+ TL +F PVF+V++R
Sbjct: 1010 MVSATLLAIFFVPVFFVVIRRC 1031



Score = 89.9 bits (223), Expect = 3e-20
Identities = 64/325 (19%), Positives = 127/325 (39%), Gaps = 14/325 (4%)

Query: 733 QLDVDLDRVKAKQLGVPVTDVFDTL-----QIYLGSMYVNDFNRFGRVFQVRAQADAPFR 787
+ + LD + + DV + L QI G G+ A F+
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ-LGGTPALPGQQLNASIIAQTRFK 241

Query: 788 AHPDDILQLKTRSD-SGQMVPLSALVDVKQTFGP-EMVVRYNGYTAADINGGPAPGYSS- 844
P++ ++ R + G +V L + V+ ++ R NG AA + A G ++
Sbjct: 242 N-PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 845 DQAQDAAERIA--AETLPRGVKFEWT-DLTYQQILAGNAGIWVFPISVLLVFLVLAALYE 901
D A+ ++A P+G+K + D T L+ + + +++LVFLV+ +
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 902 SLTLPLAVILIVPMSILAALTGVWLTSGDNNIFTQIGLMVLVGLSAKNAILIVE-FAREL 960
++ L + VP+ +L + N T G+++ +GL +AI++VE R +
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 961 EMQGSTPLQAAIEASRLRLRPILMTSIAFIMGVVPLVLSSGAGSEMRHAMGVAVFFGMLG 1020
P +A ++ ++ ++ +P+ G+ + + + M
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 1021 VTLFGLFLTPVFYVLLRTLSARKLH 1045
L L LTP L + + H
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHH 505


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4510RTXTOXIND349e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 34.4 bits (79), Expect = 9e-04
Identities = 23/192 (11%), Positives = 56/192 (29%), Gaps = 36/192 (18%)

Query: 74 TLNALETQAQQANHSLQAAAARLKQAR--ALLGNARSEQFPTVDAGFGPTRQRPSPASQG 131
L AL +A ARL+Q R L + + P + P Q
Sbjct: 126 KLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKL-------PDEPYFQN 178

Query: 132 LSANDSTDPSTLWRAQVGVSYEVDLFGRVASTVDAATADVQQSEALYRSVLLALQADVAQ 191
+S + V T+ +++ + +++ + ++ +
Sbjct: 179 VSEEE---------------------------VLRLTSLIKEQFSTWQNQKYQKELNLDK 211

Query: 192 AYFQVRELDAGLQLYRQTVELRAETLQLIQRRYDAGDISELDLARARSELESARSEALGF 251
+ + A + Y + L I++ + ++ A +E +
Sbjct: 212 KRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVY 271

Query: 252 ERRRANAEHALA 263
+ + E +
Sbjct: 272 KSQLEQIESEIL 283


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4515TCRTETB491e-08 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 49.5 bits (118), Expect = 1e-08
Identities = 56/331 (16%), Positives = 111/331 (33%), Gaps = 50/331 (15%)

Query: 26 LPEVGADLGVSLSSAGLLVTGYALGVVVGAPPVAILTTRMPRKTLLLALMVIFTLGNLAC 85
LP++ D +S + T + L +G L+ ++ K LLL ++I G++
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 86 ALAPGYGT-LMAARVLTSLAHGAFFGVGSVVATSLVKPEKQASAIALMFTGLTLANVLGV 144
+ + + L+ AR + AF + VV + E + A L+ + + + +G
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 145 PFGTWLGQAWGWRATFWAVTVVGIVAMLAIATWVPRSRGDRGGDLMG------------- 191
G + W + I + R D+ G
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFML 216

Query: 192 ----------------------ELRALSRPQV----------LLGFAMTVLGFGGVFTAF 219
+R ++ P V ++G + FG V
Sbjct: 217 FTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV 276

Query: 220 TYIAPLLTELAGFSPGAVSPILLLFGVGLVAGNTY-GGKLADR---RLMPTLVGSLALLA 275
+ + ++ ++ S + +++ G V Y GG L DR + + + ++
Sbjct: 277 SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVS 336

Query: 276 IVLAVFSLTVHAQFAAVATVAVLGAAAFATV 306
+ A F L + F + V VLG +F
Sbjct: 337 FLTASFLLETTSWFMTIIIVFVLGGLSFTKT 367


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4517DHBDHDRGNASE1172e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 117 bits (295), Expect = 2e-33
Identities = 82/263 (31%), Positives = 127/263 (48%), Gaps = 11/263 (4%)

Query: 67 LNGKVTVVTGGSKGIGLAVANAFAAEGAYVAIIARNPEGLEQARAQLQASGHNVAAYAAD 126
+ GK+ +TG ++GIG AVA A++GA++A + NPE LE+ + L+A + A+ AD
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 127 LSDPQAAAQAIERIETEIGPIDILVNSAGAARRHQPEDLDPAKWRAAMDAKFFPYIHVQD 186
+ D A + RIE E+GPIDILVN AG R L +W A +
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 187 AVLPRMRARAANRPAGATNGIVVNIIGNGGKRPTSIHLAGGSANAALMLSTVGLAAHYAQ 246
+V M R +G +V + N P + A S+ AA ++ T L A+
Sbjct: 126 SVSKYMMDR--------RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAE 177

Query: 247 YGIRINAINPGPIFTQRVEQA-LDLDASQRGITRDAALAENQAHIPLGRYGKAEEVADVA 305
Y IR N ++PG T D + +++ I + IPL + K ++AD
Sbjct: 178 YNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSL--ETFKTGIPLKKLAKPSDIADAV 235

Query: 306 LFLASARASYVVGAIIPMDGGAS 328
LFL S +A ++ + +DGGA+
Sbjct: 236 LFLVSGQAGHITMHNLCVDGGAT 258


67Bpet4542Bpet4614Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4542-116-3.192471peptide chain release factor 1
Bpet4543-125-5.296742glutamyl-tRNA reductase
Bpet4544239-9.461430*phage-related integrase
Bpet4545440-9.922563transcriptional regulator
Bpet4546038-10.104495hypothetical protein
Bpet4547-138-9.783630hypothetical protein
Bpet4548-139-9.675734hypothetical protein
Bpet4549038-8.677047putative NADPH-dependent FMN reductase
Bpet4550140-8.635499arsenate reductase
Bpet4551038-8.146524putative sodium bile acid symporter family
Bpet4552-127-3.864121hypothetical protein
Bpet4553-224-3.330464putative transcriptional regulator
Bpet4554-131-4.759098hypothetical protein
Bpet4555-133-5.096849hypothetical protein
Bpet4556032-4.721908putative DNA repair protein
Bpet4557031-3.504984ParB-like nuclease
Bpet4558439-5.412598hypothetical protein
Bpet4559338-4.462432hypothetical protein
Bpet4560229-1.695456transcriptional regulator
Bpet4561428-0.650903hypothetical protein
Bpet4562528-0.344071hypothetical protein
Bpet4563227-1.528377hypothetical protein
Bpet4564135-3.487118putative replication protein
Bpet4565043-5.305683putative partition protein
Bpet4566043-5.988139hypothetical protein
Bpet4567045-6.793652conjugal transfer protein TraF
Bpet4568246-7.928614conjugal transfer protein VirD2
Bpet4569451-9.229127putative multicopper oxidase
Bpet4570349-9.202827putative heavy-metal transporting P-type ATPase
Bpet4571449-9.499620hypothetical protein
Bpet4572448-9.880126putative membrane fusion protein silB precursor
Bpet4573347-10.214362AcrB/AcrD/AcrF family protein
Bpet4574-151-9.343921cation efflux system protein cusF precursor
Bpet4575-150-8.988165hypothetical protein
Bpet4576048-8.311211hypothetical protein
Bpet4577147-8.143781hypothetical protein
Bpet4578249-8.139088hypothetical protein
Bpet4579052-8.946132putative Thiol:disulfide interchange protein
Bpet4580150-9.949591hypothetical protein
Bpet4581144-9.570471metal-binding protein
Bpet4582145-10.148224putative cytochrome c
Bpet4583245-11.352673hypothetical protein
Bpet4584147-11.095065putative copper resistance protein D
Bpet4585347-11.275328putative transposase
Bpet4586449-11.244607transposase
Bpet4587454-11.667013copper resistance protein C precursor
Bpet4588456-11.756181hypothetical protein
Bpet4589456-11.266188putative secreted protein
Bpet4590354-11.214922copper resistance protein B precursor
Bpet4591353-11.261088copper resistance protein A precursor
Bpet4592255-11.690240copper tolerance protein
Bpet4593257-12.219728two-component sensor kinase
Bpet4594258-12.153788two-component response regulator
Bpet4595153-10.704913hypothetical protein
Bpet4596047-8.843740putative glycosylttransferase
Bpet4597047-8.273024hypothetical protein
Bpet4598150-8.707090Zinc transporter
Bpet4599251-8.786425hypothetical protein
Bpet4600150-8.908979putative transposase
Bpet4601257-10.811669putative transposase
Bpet4602462-11.538725hypothetical protein
Bpet4603465-11.559012putative dolichyl-phosphate-mannose-protein
Bpet4604465-11.904813hypothetical protein
Bpet4605465-12.227300hypothetical protein
Bpet4606464-12.226708AcrB/AcrD/AcrF family transporter
Bpet4607464-12.260706cobalt-zinc-cadmium resistance protein czcB
Bpet4608365-13.362389metal ion efflux outer membrane protein,
Bpet4609156-12.333899two-component response regulator
Bpet4610145-9.044006two-component sensor kinase
Bpet4611138-6.941297two-component response regulator
Bpet4612133-5.225825Outer membrane porin protein precursor
Bpet4613229-3.701950hypothetical protein
Bpet4614328-2.839859LysR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4553TETREPRESSOR270.024 Tetracycline repressor protein signature.
		>TETREPRESSOR#Tetracycline repressor protein signature.

Length = 218

Score = 27.2 bits (60), Expect = 0.024
Identities = 11/26 (42%), Positives = 19/26 (73%)

Query: 65 GLEGLTPSVLAEQLAVARNTLSFHLK 90
G++GLT LA++L + + TL +H+K
Sbjct: 21 GIDGLTTRKLAQKLGIEQPTLYWHVK 46


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4572RTXTOXIND501e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 49.8 bits (119), Expect = 1e-08
Identities = 34/147 (23%), Positives = 49/147 (33%), Gaps = 16/147 (10%)

Query: 200 DDLIERVERTRKAQPTLTIRAPSTGLLQTLNVR-NGMAVSAGTVLAQLNGLDAV-WLDAA 257
L + + + Q IRAP + +Q L V G V+ L + D + A
Sbjct: 312 GLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTAL 371

Query: 258 VPETQARAMRPGLEAKVSFPAFPG---HTVVGKVSYILPEVDAASRT-----ARIRIELQ 309
V + G A + AFP +VGKV I + R I IE
Sbjct: 372 VQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEEN 431

Query: 310 -----NPDGQLRPGLFAKVEFAHTGEK 331
N + L G+ TG +
Sbjct: 432 CLSTGNKNIPLSSGMAVTA-EIKTGMR 457



Score = 33.6 bits (77), Expect = 0.002
Identities = 15/56 (26%), Positives = 28/56 (50%), Gaps = 3/56 (5%)

Query: 217 TIRAPSTGLLQTLNVRNGMAVSAGTVLAQLNGLDAVWLDAAVPETQARAMRPGLEA 272
I+ +++ + V+ G +V G VL +L L A +A +TQ+ ++ LE
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGA---EADTLKTQSSLLQARLEQ 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4573ACRIFLAVINRP6850.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 685 bits (1769), Expect = 0.0
Identities = 222/1058 (20%), Positives = 437/1058 (41%), Gaps = 50/1058 (4%)

Query: 5 LIRWSINNRFLVLLATLFLFFWGLWAIKNAPIDALPDLSDVQVIVRTTYPGQAPQIVENQ 64
+ + I + + L G AI P+ P ++ V V YPG Q V++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 65 VTYPLATTMLSVPGAKTVRGYSF-FGDSFVYIIFEDGTDLYWARSRVLEYLNQVQGRLPA 123
VT + M + + S G + + F+ GTD A+ +V L LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 124 TAK-PSLGPDATGVGWIYEYALVDRSGNHDLSQLRGLQDWFLKYELKSVPDVAEVASIGG 182
+ + + + ++ V + + +K L + V +V G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 183 MVKQYQIVIDPIKLAAFGVTQDQVIQAVRASNQEVGG------SVLELAEAEYMVRASGY 236
+I +D L + +T VI ++ N ++ L + + A
Sbjct: 181 Q-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 237 LASLEDFRNIPLAVKSGGTPILLGDVATIQLGPEIRRGIAELNGEGETAGGVVILRSGKN 296
+ E+F + L V S G+ + L DVA ++LG E IA +NG+ AG + L +G N
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGK-PAAGLGIKLATGAN 298

Query: 297 ARQAISAVKAKLDELKASLPPGVEIVPTYDRSKLIDRAIENLTSKLIEEFIVVALVCAIF 356
A A+KAKL EL+ P G++++ YD + + +I + L E ++V LV +F
Sbjct: 299 ALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLF 358

Query: 357 LWHLRSALVAIISLPLGVLTAFIIMHYQGVSANIMSLGGIAIAIGAMVDAAVVMIENAHK 416
L ++R+ L+ I++P+ +L F I+ G S N +++ G+ +AIG +VD A+V++EN +
Sbjct: 359 LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVER 418

Query: 417 HVETWRHEHLGQELRGEAHWRVMANAAAEVGPALFFSLLIITLSFIPVFTLQAQEGRLFG 476
+ + +++ AL ++++ FIP+ G ++
Sbjct: 419 VMME----------DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYR 468

Query: 477 PLAFTKTYAMAGAAGLAVTLVPVLMGYWIRGRIPP--EEKNPLNRFLIRIYRPLLD---- 530
+ T AMA + +A+ L P L ++ E K + + ++
Sbjct: 469 QFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTN 528

Query: 531 ---AVLRRPRTTLAAALIILLTAIWPATRLGGEFLPPLDEGDLLYMPSALPGLSASKASE 587
+L L +I+ + RL FLP D+G L M G + + +
Sbjct: 529 SVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQK 588

Query: 588 LL-QLTDR-MIRTVPEVASVFGKAGRAETATDPAPLEMFETTVQLKPHDQW-RPGMTVDK 644
+L Q+TD + V SVF G + + F V LKP ++ + +
Sbjct: 589 VLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAF---VSLKPWEERNGDENSAEA 645

Query: 645 LIQALDDAVKVPGLANIWVPPIRNRLDML-ATGIKSPIGVKVAAANLADIESVALAIEQV 703
+I + + + +++ ATG + + + A ++ +
Sbjct: 646 VIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMA 705

Query: 704 AKTVPGVSSALAERLTGGRYVDVQVDRISAARYGLSIADVQSVVSAAVGGENIGETVEGL 763
A+ + S L ++VD+ A G+S++D+ +S A+GG + + ++
Sbjct: 706 AQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRG 765

Query: 764 ARYPINVRYPREIRDSVEGLRNLPMFTSNGQQITLGTVARIAIENGPPMLKSENARPTGW 823
+ V+ + R E + L + ++NG+ + G P L+ N P+
Sbjct: 766 RVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPS-- 823

Query: 824 VYIDVRGRDLASVVSDLRHAVAQQVA--LKPGMSISFSGQFEYMERATARLTLVIPATLL 881
++++G S A+ + +A L G+ ++G + + ++ + +
Sbjct: 824 --MEIQGEAAPGTSSGDAMALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFV 881

Query: 882 IIFVLLYLTFRRFDEALLIMATLPFALVGGVWFLYLLNYHLSVPAGIGFIALAGVSAEFG 941
++F+ L + + + +M +P +VG + L N V +G + G+SA+
Sbjct: 882 VVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNA 941

Query: 942 VVMLFYLKQAAASRQDSGLSLTPSVLEDAIREGAVMRVRPKAMTVAVILAGLLPILLGSG 1001
++++ + K + G + +A MR+RP MT + G+LP+ + +G
Sbjct: 942 ILIVEFAKDL---MEKEGKG-----VVEATLMAVRMRLRPILMTSLAFILGVLPLAISNG 993

Query: 1002 AGSEIMSRIAAPMVGGMITAPLLSMFVIPAAYRLMRGR 1039
AGS + + ++GGM++A LL++F +P + ++R
Sbjct: 994 AGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4580BINARYTOXINB300.004 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 30.0 bits (67), Expect = 0.004
Identities = 18/81 (22%), Positives = 35/81 (43%), Gaps = 11/81 (13%)

Query: 29 LQTSLDSPAGGITT-YTEFADSANHVKASLTKTGQDG---VITLHIGDGWHVNAN----- 79
+ S G ++ ++ S + SL+ G+ + L+ D +NAN
Sbjct: 338 VHASFFDIGGSVSAGFSNSNSSTVAIDHSLSLAGERTWAETMGLNTADTARLNANIRYVN 397

Query: 80 --PASLDNLIPTSVLVVGDGE 98
A + N++PT+ LV+G +
Sbjct: 398 TGTAPIYNVLPTTSLVLGKNQ 418


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4591cloacin320.008 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 32.0 bits (72), Expect = 0.008
Identities = 27/114 (23%), Positives = 42/114 (36%), Gaps = 7/114 (6%)

Query: 375 AHDMSGMDMGGESGGSMKG--MDHGSMSNADQSSSGAGNQGAMSGMDHGSMGGMAGMSHG 432
AH SG GG +G + G D S+ + G G G G G + G
Sbjct: 13 AHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSG 72

Query: 433 GMNHGAGDGEGGMVEVKHPYPAENSP-----ATTMPPDVVSTRLDDPGVGLRGN 481
G + G+ V +PA ++P A ++ +S + D L+G
Sbjct: 73 GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKGP 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4594HTHFIS788e-19 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 77.6 bits (191), Expect = 8e-19
Identities = 41/126 (32%), Positives = 65/126 (51%), Gaps = 1/126 (0%)

Query: 2 RILVIEDERKLAHYLQKGLTEHNYVVDIASNGVDGRHAALEGNYDLVVLDVMLPGIDGFW 61
ILV +D+ + L + L+ Y V I SN G+ DLVV DV++P + F
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 ILKDLRET-KDTPVLMLTARDKVEDRVRGLENGADDYLVKPFAFSELLARIQALLRRGRG 120
+L +++ D PVL+++A++ ++ E GA DYL KPF +EL+ I L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 121 QESTLL 126
+ S L
Sbjct: 125 RPSKLE 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4595ENTEROVIROMP280.008 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 27.9 bits (62), Expect = 0.008
Identities = 16/46 (34%), Positives = 20/46 (43%), Gaps = 4/46 (8%)

Query: 1 MKRISCAIVAAAISFGAIGIVHAETKT----RAQVRAELQEAKAKG 42
MK+I+C AA+ G A T T AQ A+ Q K G
Sbjct: 1 MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKMGG 46


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4596PF06057280.045 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 28.3 bits (63), Expect = 0.045
Identities = 16/130 (12%), Positives = 40/130 (30%), Gaps = 20/130 (15%)

Query: 178 VRHYRKRWIEPRRSGRLVVGSNAGTADYKRWLDMVEGA-ALLPKHLRDQI--IILIAGVP 234
+ Y+ + +++G Y +++ +P R + +L++
Sbjct: 107 IDKYQAEF---GTQKVILIG-------YSFGAEVIPFVLNEMPARYRKNVLGAVLLSPSQ 156

Query: 235 PSEDQVSKVEQLGMMDHVIFVGLLDDVRPFIATLDVGFVL---SSEVETISFACREMMAM 291
S+ ++ E + + P + +L E + C E+
Sbjct: 157 SSDFEIHVSEMVTSDNQ----SARYLTLPEVNKQTTVPMLCLYGKEDDAPLHLCPEVKQP 212

Query: 292 GVPVIVSDSG 301
V V+ G
Sbjct: 213 NVTVMELSGG 222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4606ACRIFLAVINRP6490.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 649 bits (1677), Expect = 0.0
Identities = 224/1077 (20%), Positives = 413/1077 (38%), Gaps = 78/1077 (7%)

Query: 8 LSVRARWAVLFLFLAIGALGVWQLTKLPIDAVPDITNNQVQINTVDPRLSPVEIEKLVTY 67
+R L + + G + +LP+ P I V ++ P ++ VT
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 68 PVEISLAGIPGLESTRSIS-RNGFSQVTAIFTDKTDLYFARQQVGERLIKAQESLPDGVQ 126
+E ++ GI L S S G +T F TD A+ QV +L A LP VQ
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 127 PQIGPVTTGLGEVLLYTVGYTYPDGEGAQKVAGEPGWQPDGSYLTPEGDRMVDEVAKAGY 186
Q V L+ G+ + Q
Sbjct: 124 QQGISVEKSSSSYLMV-AGFVSDNPGTTQ-----------------------------DD 153

Query: 187 LRTVQDWIVAPQLKALPGVAGVDSIGGYAKTFVVEPNPTKLASYGISYSELGEALERANI 246
+ V L L GV V G + + L Y ++ ++ L+ N
Sbjct: 154 ISDYVASNVKDTLSRLNGVGDVQLFGA-QYAMRIWLDADLLNKYKLTPVDVINQLKVQND 212

Query: 247 AVGANYYNRGGEA------YLVRVDARVGSVDEIRN-AVAATRGGVPITVGQIADVKIGG 299
+ A + R + +E + G + + +A V++GG
Sbjct: 213 QIAAGQLGGTPALPGQQLNASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGG 272

Query: 300 DLRTGAGSMNGEEAVIGTVLMLIGENSRVVAEDVSAKLDQIATSLPPGIQVKTVLDRAKL 359
+ +NG+ A + + G N+ A+ + AKL ++ P G++V D
Sbjct: 273 ENYNVIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPF 332

Query: 360 VNATVSTVERNLTEGAILVAASLFLLLGNWRAALIAVLVIPFSFLMMAMGMNAFKVPGNL 419
V ++ V + L E +LV ++L L N RA LI + +P L + AF N
Sbjct: 333 VQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT 392

Query: 420 MSLG--ALDFGLIVDGSVIIIENCLARLAHRQQHEGRLLFLRERLEETMRAAQEMIKPTV 477
+++ L GL+VD +++++EN R E +L E T ++ ++ V
Sbjct: 393 LTMFGMVLAIGLLVDDAIVVVENV-----ERVMMEDKL----PPKEATEKSMSQIQGALV 443

Query: 478 FGQVIILLTFAPLLMFTGVEGKTFSPMAITIMLALVAAFILAITLVPALVAILIRGRVAE 537
+++ F P+ F G G + +ITI+ A+ + ++A+ L PAL A L++ AE
Sbjct: 444 GIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAE 503

Query: 538 KE-------VWLI---AKSKERYLPFLDKAIARPWPFIFAGLVFFLAAIPAFGLLGSEFI 587
W S Y + K + ++ + + F L S F+
Sbjct: 504 HHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFL 563

Query: 588 PKLDEKNLAVASTRVPSVSLEQSLAMQLKVEDAIKKLPEVELMFSKTGTAEVATDPMPPN 647
P+ D+ + E++ + +V D K + + S + N
Sbjct: 564 PEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANV-ESVFTVNGFSFSGQAQN 622

Query: 648 VSDGFVILKPQEEWPDGVTTKAQVIERV-EKAAGTQLGNLYEVSQPIELRFNELIAGVRG 706
FV LKP EE + VI R + + G + + P EL
Sbjct: 623 AGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMP---AIVELGTATGF 679

Query: 707 DVA-IKLYGDDLEKMQQTANEMVRVLQDIPGA-GSVKADQVGGAPTLDVKLNRAEIARYG 764
D I G + + Q N+++ + P + SV+ + + +++++ + G
Sbjct: 680 DFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALG 739

Query: 765 LTVQDVADTIAAALGGRPSGLLYEGDRRFDITVRVPEATRMNLDAIRALPILLPEMEGQL 824
+++ D+ TI+ ALGG + R + V+ RM + + L + G++
Sbjct: 740 VSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYV--RSANGEM 797

Query: 825 RRQVPLARVAQIRLTEGLNEIRRENGKRRVVVQVNLDGRDAGSFVEEAQAKIAQV--QLP 882
VP + G + R NG + +Q G+ +A A + + +LP
Sbjct: 798 ---VPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA---APGTSSGDAMALMENLASKLP 851

Query: 883 AGYYLEWGGQFESLQAASQRLSIVVPICFLAIFVLLFMALGGFGRALSVFLAVPLGLAGG 942
AG +W G + + + +V I F+ +F+ L + +SV L VPLG+ G
Sbjct: 852 AGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGV 911

Query: 943 VFTLAMTGINFSVSAAVGFICLAGVAVLNGLVVMT-AIRAHTEAGLPLSEAIREGMKEKM 1001
+ + V VG + G++ N ++++ A + G + EA ++ ++
Sbjct: 912 LLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRL 971

Query: 1002 RAVVMTGFVPAIGFVPMALALGTGAEVQKPLATTVIGGLIAATILTLLVLPAIAKVV 1058
R ++MT +G +P+A++ G G+ Q + V+GG+++AT+L + +P V+
Sbjct: 972 RPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVI 1028



Score = 109 bits (274), Expect = 3e-26
Identities = 101/534 (18%), Positives = 202/534 (37%), Gaps = 46/534 (8%)

Query: 557 AIARPWPFIFAGLVFFLAAIPAFGLLGSEFIPKLDEKNLAVASTRVPSVS---LEQSLAM 613
I RP ++ +A A L P + ++V S P ++ ++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSV-SANYPGADAQTVQDTVTQ 63

Query: 614 QLKVEDAIKKLPEVELMFSKTGTAEVATDPMPPNVSDGFVILKPQEEWPDGVTTKAQVIE 673
+E + + + M S + +A T ++ F + D + QV
Sbjct: 64 --VIEQNMNGIDNLMYMSSTSDSAGSVT------ITLTF------QSGTDPDIAQVQVQN 109

Query: 674 RVEKAAGTQLGNLYEVSQPIELRFNELIAGVRGDVAIKLYGDDLEKMQQT---ANEMVRV 730
+++ A L + Q + + + + + A+ +
Sbjct: 110 KLQLA----TPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDT 165

Query: 731 LQDIPGAGSVKADQVGGAPTLDVKLNRAEIARYGLTVQDVADTIAAAL----GGRPSGLL 786
L + G G V G + + L+ + +Y LT DV + + G+ G
Sbjct: 166 LSRLNGVGDV--QLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTP 223

Query: 787 YEGDRRFDITVRVPEATRMNLDAIRALPILLPEMEGQLRRQVPLARVAQIRL-TEGLNEI 845
++ + ++ + N + + + V L VA++ L E N I
Sbjct: 224 ALPGQQLNASIIA-QTRFKNPEEFGKVTLR----VNSDGSVVRLKDVARVELGGENYNVI 278

Query: 846 RRENGKRRVVVQVNL-DGRDAGSFVEEAQAKIAQVQ--LPAGYYLEWGGQFESLQAASQR 902
R NGK + + L G +A + +AK+A++Q P G ++ +++
Sbjct: 279 ARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQG--MKVLYPYDTTPFVQLS 336

Query: 903 LSIVVPICFLAI---FVLLFMALGGFGRALSVFLAVPLGLAGGVFTLAMTGINFSVSAAV 959
+ VV F AI F+++++ L L +AVP+ L G LA G + +
Sbjct: 337 IHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMF 396

Query: 960 GFICLAGVAVLNGLVVMTAI-RAHTEAGLPLSEAIREGMKEKMRAVVMTGFVPAIGFVPM 1018
G + G+ V + +VV+ + R E LP EA + M + A+V V + F+PM
Sbjct: 397 GMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPM 456

Query: 1019 ALALGTGAEVQKPLATTVIGGLIAATILTLLVLPAIAKVVLEPKEKRKSDIPEG 1072
A G+ + + + T++ + + ++ L++ PA+ +L+P + G
Sbjct: 457 AFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGG 510



Score = 88.0 bits (218), Expect = 1e-19
Identities = 52/322 (16%), Positives = 116/322 (36%), Gaps = 24/322 (7%)

Query: 218 FVVEPNPTKLASYGISYSELGEALERANIAVGANYYNRGGEAYLVRV---DARVGSVDEI 274
F +E + K + G+S S++ + + A N + G + V +++
Sbjct: 726 FKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDV 785

Query: 275 RNAVAATRGGVPITVGQIADVKIGGDLRTGAGSMNGEEAVIGTVLMLIGENSRVVAEDVS 334
+ G + G+ + + + + D
Sbjct: 786 DKLYVRSANGEMVPFSAFTTSHWV----YGSPRLERYNGLPSMEI-QGEAAPGTSSGDAM 840

Query: 335 AKLDQIATSLPPG--IQVKTVLDRAKLVNATVSTVERNLTEGAILVAASLFLLLGNWRAA 392
A ++ +A+ LP G + + +L + + ++V L L +W
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPAL---VAISFVVVFLCLAALYESWSIP 897

Query: 393 LIAVLVIPFSFLMMAMGMNAFKVPGNLMSLGAL--DFGLIVDGSVIIIENCLARLAHRQQ 450
+ +LV+P + + + F ++ + L GL +++I+E +
Sbjct: 898 VSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVE----FAKDLME 953

Query: 451 HEGRLLFLRERLEETMRAAQEMIKPTVFGQVIILLTFAPLLMFTGVEGKTFSPMAITIML 510
EG+ + E T+ A + ++P + + +L PL + G + + I +M
Sbjct: 954 KEGKGVV-----EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008

Query: 511 ALVAAFILAITLVPALVAILIR 532
+V+A +LAI VP ++ R
Sbjct: 1009 GMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4607RTXTOXIND492e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 48.7 bits (116), Expect = 2e-08
Identities = 33/200 (16%), Positives = 60/200 (30%), Gaps = 30/200 (15%)

Query: 125 ASRDAAQLAAARTAAYARAELARKELAREQYLYKQQVSARVDLERAQAEAQAAAADARRA 184
A + + + A++E L+K ++ + Q A
Sbjct: 264 AVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILD----KLRQTTDNIGLLTLELA 319

Query: 185 KVEAEVANVTKDGQGVAVSSPISGRITTQSL-SLGAFVQPETELFRIA-DPKQIQVEAAI 242
K E + +P+S ++ + + G V L I + ++V A +
Sbjct: 320 KNEERQQASV-------IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 243 LPSDIFRIAPGDRAIVELPG-----GGTLEAKVGSVTPSLNTATRQ------------AT 285
DI I G AI+++ G L KV ++ R
Sbjct: 373 QNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENC 432

Query: 286 AVIDVEAGSLQPGLAVRVRI 305
+ L G+AV I
Sbjct: 433 LSTGNKNIPLSSGMAVTAEI 452



Score = 36.7 bits (85), Expect = 1e-04
Identities = 22/141 (15%), Positives = 48/141 (34%), Gaps = 17/141 (12%)

Query: 76 LDAEIGAQAVVSPQPGGEAIVTARASGAVTQVFKRLGDPVQAGEVLA-VVASRDAAQLAA 134
++ A ++ G + + V ++ + G+ V+ G+VL + A A
Sbjct: 80 VEIVATANGKLTHS-GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 135 ARTA-AYARAELARKELAREQ-------------YLYKQQVSAR-VDLERAQAEAQAAAA 179
+++ AR E R ++ Y Q VS V + + Q +
Sbjct: 139 TQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTW 198

Query: 180 DARRAKVEAEVANVTKDGQGV 200
++ + E + + V
Sbjct: 199 QNQKYQKELNLDKKRAERLTV 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4609HTHFIS877e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 7e-22
Identities = 40/142 (28%), Positives = 74/142 (52%), Gaps = 4/142 (2%)

Query: 1 MGKLKILVIEDERKLAEYLKRALSEHNYVVDIAMDGISGLHLAQETQYDLILLDVMLPGR 60
M ILV +D+ + L +ALS Y V I + + DL++ DV++P
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 DGFSVLAELRKGD-RVPILMLTARDKLEDRVRGLQDGADDYLAKPFALSELLA---RVLA 116
+ F +L ++K +P+L+++A++ ++ + GA DYL KPF L+EL+ R LA
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 117 LSRRQSNSVIEPNRQNVLKVGD 138
+R+ + + + ++ + VG
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGR 142


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4611HTHFIS672e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.8 bits (163), Expect = 2e-15
Identities = 27/98 (27%), Positives = 53/98 (54%), Gaps = 5/98 (5%)

Query: 2 QYDLIILDINLPDMEGFEVLQRIRQSDA-VPVMMLTARTSLEDRVRGLEQGADDYLAKPF 60
DL++ D+ +PD F++L RI+++ +PV++++A+ + ++ E+GA DYL KPF
Sbjct: 47 DGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106

Query: 61 ALSELQARVQALRRRGSGNESRRGPNVLRVADLELDLL 98
L+E + + R RR + + + L+
Sbjct: 107 DLTE----LIGIIGRALAEPKRRPSKLEDDSQDGMPLV 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4612ECOLNEIPORIN815e-19 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 80.6 bits (199), Expect = 5e-19
Identities = 78/390 (20%), Positives = 134/390 (34%), Gaps = 64/390 (16%)

Query: 1 MKKTILLAASAATFSAAAVHAETSVTLYGLIDTGIGYAKVDGSYTNPNTGAKADVNASRI 60
MKK+++ A T +A V A VTLYG I G+ T+ + AS
Sbjct: 1 MKKSLI----ALTLAALPVAAMADVTLYGTIKAGV--------ETSRSVAHNGAQAASVE 48

Query: 61 GATTGQTAGSRWGLRGKEDLGDGLYATFRLESGFDSTNGESSQGGRLFGREATVGLGSAD 120
T GS+ G +G+EDLG+GL A +++E +S G R + +GL
Sbjct: 49 TGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQ----SFIGL-KGG 103

Query: 121 WGEVRLGRQYNVASRMMGSLFGNQFGGFTQLTTGAGLGFSGSNWVRYDNL---ALYESPS 177
+G++R+GR +V + G + + Y+SP
Sbjct: 104 FGKLRVGRLNSVL----------KDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPE 153

Query: 178 FGGFRLSAGYSFNANDLSAAQSGFATADNTRAITSGLSYNNGPLLAFIAYEQLNASNKLS 237
F G S Y+ N N + N + + Y A+ + Q+ + +
Sbjct: 154 FAGLSGSVQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGG----AYKRHHQVQENVNIE 209

Query: 238 NAQTSATPRSFTVGAAYDFEVLKLIAAYERATDGWFAGKGLPSGANINGFQGTPSNAFVD 297
Q + A Y +A ++ ++ + + + A+
Sbjct: 210 KYQIHRLVSGYDNDALYAS-----VAVQQQDAKLVEENY-----SHNSQTEVAATLAYRF 259

Query: 298 GFSTN--SYLLGVAVPLGGASSMFGSWQRVDPNNSDLTGGDSTSNTFALGYSYKLSKRTN 355
G T SY G ++ + +G Y SKRT+
Sbjct: 260 GNVTPRVSYAHGFKGSFDAT------------------NYNNDYDQVVVGAEYDFSKRTS 301

Query: 356 IYAAGSYTKNFAFQSDAKATEAIIGLRHVF 385
+ + + +S +T +GLRH F
Sbjct: 302 ALVSAGWLQEGKGESKFVSTAGGVGLRHKF 331


68Bpet4623Bpet4638Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4623034-5.156627conjugal transfer protein TrbL
Bpet4624242-7.418048conjugal transfer protein TrbF
Bpet4625153-9.815802conjugal transfer protein TrbG
Bpet4626156-11.012409conjugal transfer protein TrbI
Bpet4627366-13.656125hypothetical protein
Bpet4628261-12.493498hypothetical protein
Bpet4629257-11.578918hypothetical protein
Bpet4630352-10.708907hypothetical protein
Bpet4631433-7.390576hypothetical protein
Bpet4632232-6.343866hypothetical protein
Bpet4633136-7.609317transposase
Bpet4634339-9.129586ISxcc1 transposase
Bpet4635239-8.266274transposase
Bpet4636-133-5.953116transposase
Bpet4637-230-5.881347hypothetical protein
Bpet4638-126-5.528872hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4625TYPE4SSCAGX330.002 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 32.8 bits (74), Expect = 0.002
Identities = 53/205 (25%), Positives = 75/205 (36%), Gaps = 36/205 (17%)

Query: 43 IVPMPQPLPLPGQLKAL-KDKPAPEQTSPTAQVNRANSEARMAPTR---DGFINAIQ--- 95
IV P P L Q KAL K+K A EQ + R + A R + NA+
Sbjct: 132 IVDAPDPKELEEQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANLENLTNAMSNPQ 191

Query: 96 -----------VWPYSEGALYQVYASPGRVTLVQ---LQVGERLIDVSAGDTVRWIVGDS 141
+ E L Q+ Q L+ E L A + VR D
Sbjct: 192 NLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVRQRAKDK 251

Query: 142 SS-----GTGATARPNLQIKPIRAGLKTNLVVTTDRRIYLLELASTQSAWMAS----VSW 192
S + ++++ P + +TNLVV T++ +Y L Q AS V
Sbjct: 252 ISIKTDKSQKSPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAYLTVKL 311

Query: 193 DYPQDRLAA------LKRRNEAAAQ 211
+YPQ + LK+R EA Q
Sbjct: 312 EYPQRHEVSSVIEEELKKREEAKRQ 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4634PF08280260.014 M protein trans-acting positive regulator
		>PF08280#M protein trans-acting positive regulator

Length = 530

Score = 26.3 bits (58), Expect = 0.014
Identities = 9/30 (30%), Positives = 16/30 (53%)

Query: 15 LKEGETGVPVADLCRKHGISNATYYQWKSK 44
+K G P+ D R H +SN++ Y+ +
Sbjct: 129 IKNGSHSRPLTDFARSHFLSNSSAYRMREA 158


69Bpet4693Bpet4711Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4693-2183.501639putative peptidase
Bpet4694-2211.808779phosphoglyceromutase
Bpet4695-1203.049362hypothetical protein
Bpet4696-1183.213820glutaredoxin 3
Bpet4697-1182.599206preprotein translocase subunit SecB
Bpet46980153.463939NAD(P)H-dependent glycerol-3-phosphate
Bpet46990123.211367putative secreted protein
Bpet47000113.010485hypothetical protein
Bpet4701-1101.910770rRNA methylase
Bpet4702-2101.682299aldehyde dehydrogenase family protein
Bpet4703-182.094668hypothetical protein
Bpet4704-291.360562hypothetical protein
Bpet4705-2100.527856carboxylate-amine ligase
Bpet4706-2111.706385Trk system potassium uptake protein
Bpet4707-2112.664948potassium transporter peripheral membrane
Bpet4708-293.019242nitrogen regulation protein NR(I)
Bpet4709-2103.051356putative two-component sensor kinase
Bpet4710-2114.286240hypothetical protein
Bpet4711-1103.803156hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4693IGASERPTASE556e-10 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 54.7 bits (131), Expect = 6e-10
Identities = 38/226 (16%), Positives = 82/226 (36%), Gaps = 22/226 (9%)

Query: 153 SRARAQAVQALRHDIDRL--AQLQGQADARRADIQTMAAEAADQKTQLVAQQKERATLLA 210
+A +V + +I R+ A + A A ++ AE + Q+++ V + ++ AT
Sbjct: 1003 IQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETT 1062

Query: 211 RLEGQIAAQRAEARQLGRDDNRLSHLIDDLEKAIAEQAEAARRAEEARRRAEAVKRAQEA 270
++A + S++ + + E A+ E + + + A
Sbjct: 1063 AQNREVAKEAK------------SNVKANTQ-----TNEVAQSGSETKETQTTETK-ETA 1104

Query: 271 RLAEEARRKAEAERRAEAARQARRNEEARDAARAREQVEAAAARERDQQREQQRGPVALA 330
+ +E + K E E+ E + + Q +A ARE D + P +
Sbjct: 1105 TVEKEEKAKVETEKTQEVPKVT-SQVSPKQEQSETVQPQAEPARENDPT-VNIKEPQSQT 1162

Query: 331 DPDAAGLRPAGSGSGRIVPPQRPAAPTDRANQAADDDSADDESSRQ 376
+ A +PA S + P + + N ++ ++ Q
Sbjct: 1163 NTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQ 1208



Score = 48.1 bits (114), Expect = 7e-08
Identities = 21/138 (15%), Positives = 46/138 (33%), Gaps = 6/138 (4%)

Query: 243 AIAEQAEAARRAEEARRRAEAVKRAQEARLAEEARRKAEAERRA-EAARQARRNEEARDA 301
+AE ++ + E + AQ +A+EA+ +A + E A+ +E +
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTT 1098

Query: 302 ARAREQVEAAAARERDQQREQQRGPVALADPDAAGLRPAGSGSGRIVPPQRPAAPTDRAN 361
+ + + + Q P + + P S + P PA D
Sbjct: 1099 ETKETATVEKEEKAKVETEKTQEVPKV-----TSQVSPKQEQSETVQPQAEPARENDPTV 1153

Query: 362 QAADDDSADDESSRQQAA 379
+ S + ++ +
Sbjct: 1154 NIKEPQSQTNTTADTEQP 1171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4697SECBCHAPRONE1405e-45 Bacterial protein-transport SecB chaperone protein ...
		>SECBCHAPRONE#Bacterial protein-transport SecB chaperone protein

signature.
Length = 170

Score = 140 bits (354), Expect = 5e-45
Identities = 49/162 (30%), Positives = 86/162 (53%), Gaps = 6/162 (3%)

Query: 1 MAEQDQN--TQQAGGDAPSFNLQRVYLKDLSLEMPNAPHVFLEQEQPQVEVSINVGGQRL 58
M+E++Q P +QR+Y+KD+S E PN PH+F + +P++ ++ +++
Sbjct: 1 MSEENQVNAADTQATQQPVLQIQRIYVKDVSFEAPNLPHIFQQDWEPKLSFDLSTEAKQV 60

Query: 59 AETVFES--TVTATVTTRINDKVLYLVEGTQAGIFELANIPAEQMDALLGIVCPTMLYPY 116
+ ++E ++ T + V ++ E QAG+F ++ + QM L CP ML+PY
Sbjct: 61 GDDLYEVCLNISVETTMESSGDVAFICEVKQAGVFTISGLEEMQMAHCLTSQCPNMLFPY 120

Query: 117 LRANVADAITRTSLPALHLAEVNFQALYEQRLAELAQQQGGN 158
R V+ + R + PAL+L+ VNF AL+ L Q+Q
Sbjct: 121 ARELVSSLVNRGTFPALNLSPVNFDALFMDYLQR--QEQAEQ 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4708HTHFIS1046e-28 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 104 bits (260), Expect = 6e-28
Identities = 44/119 (36%), Positives = 61/119 (51%), Gaps = 1/119 (0%)

Query: 2 ARILVVDDEVGIRELLSEILYDEGHTVELAENAAEARAARLRTRPDLVLLDIWMPDTDGV 61
A ILV DD+ IR +L++ L G+ V + NAA DLV+ D+ MPD +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 SLLKEWGSQGLLDMPVIMMSGHATIDTAVEATRIGAMDFLEKPITLQRLLKTIAAGLAR 120
LL D+PV++MS T TA++A+ GA D+L KP L L+ I LA
Sbjct: 64 DLLPRIKKAR-PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121



Score = 47.1 bits (112), Expect = 2e-08
Identities = 16/117 (13%), Positives = 30/117 (25%)

Query: 115 AAGLARGRAPHPAPAIAPLAPATPPVPLEDELDPPAAALAAMPAAAEAAPLSNGRLGSIA 174
L L P P+E + + ++
Sbjct: 365 LTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALP 424

Query: 175 LDQPLREARDEFERIYFEYHLVRENHSMTRVSERTGLERTHLYRKLKQLGIESARKR 231
E E L + + ++ GL R L +K+++LG+ R
Sbjct: 425 PSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS 481


70Bpet4746Bpet4752Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet47462132.189454acetyltransferase
Bpet47472133.278412histidine triad (HIT)-like
Bpet47481143.726439hypothetical protein
Bpet47490143.686642putative extracellular solute-binding protein
Bpet47500123.669777hypothetical protein
Bpet47510113.816726uracil-DNA glycosylase
Bpet4752-1113.272729putative ATP-dependent RNA helicase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4746SACTRNSFRASE401e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 39.5 bits (92), Expect = 1e-06
Identities = 21/80 (26%), Positives = 33/80 (41%), Gaps = 5/80 (6%)

Query: 60 LAYDNGRAVGMVHWVLHRSCWTAGDYCYLQDLFVAPDVRGGGHGRALIEHVYAQARAVNA 119
L Y +G + RS W Y ++D+ VA D R G G AL+ A+ +
Sbjct: 69 LYYLENNCIGRIKI---RSNWN--GYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHF 123

Query: 120 ARVYWLTHETNHTAMQLYDR 139
+ T + N +A Y +
Sbjct: 124 CGLMLETQDINISACHFYAK 143


71Bpet4781Bpet4834Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4781-2153.595417S-adenosylmethionine synthetase
Bpet4782-1123.035523lipid A biosynthesis lauroyl acyltransferase
Bpet4783-1103.083009lipid A biosynthesis lauroyl acyltransferase
Bpet4784-1103.070095diaminopimelate epimerase
Bpet4785-1112.872599hypothetical protein
Bpet47861143.034311site-specific tyrosine recombinase XerC
Bpet47871152.970465TonB-dependent outer membrane receptor
Bpet47883164.366510hypothetical protein
Bpet47890132.249493hypothetical protein
Bpet4790-2121.320846putative periplasmic solute-binding protein
Bpet4791-1140.944285putative ABC transporter permease protein
Bpet4792219-0.684024putative transport protein ATP-binding
Bpet4793324-1.679075putative regulatory protein
Bpet4794324-1.391191hypothetical protein
Bpet4795-1220.590323putative dnaK suppressor protein
Bpet4796-2192.162664ATP-dependent protease peptidase subunit
Bpet4797-3152.077588ATP-dependent protease ATP-binding subunit HslU
Bpet4798-1133.099605hypothetical protein
Bpet47990123.043565hypothetical protein
Bpet48000132.395750dTDP-glucose 4,6-dehydratase
Bpet4801-2111.799258dTDP-4-dehydrorhamnose reductase
Bpet4802-3140.836503dTDP-4-dehydrorhamnose 3,5-epimerase
Bpet4803-3151.345466lipoyl synthase
Bpet4804-2132.641693lipoate-protein ligase B
Bpet4805-1133.110062hypothetical protein
Bpet4806-1133.284591D-alanine aminotransferase
Bpet48071134.675514secreted serine-type D-Ala-D-Ala
Bpet4808-1125.696029hypothetical protein
Bpet4809-2104.714322putative biotin protein ligase
Bpet4810-292.687033pantothenate kinase
Bpet4811-281.577913hypothetical protein
Bpet4812-281.4628743-deoxy-D-manno-octulosonic-acid transferase
Bpet4813013-0.290192heptosyltransferase
Bpet4814015-0.990313lipopolysaccharides biosynthesis oxidoreductase
Bpet48152130.463410transpoase
Bpet48164111.502865transposase
Bpet48174121.324523lipopolysaccharides biosynthesis
Bpet48183111.295839lipopolysaccharides biosynthesis
Bpet48194101.288994UDP-N-acetylglucosamine 2-epimerase
Bpet4820690.879487glycosyltransferase-like membrane protein
Bpet482158-0.310504lipopolysaccharides biosynthesis
Bpet4822210-1.599353lipopolysaccharides biosynthesis
Bpet4823211-1.768089hypothetical protein
Bpet4824120-4.646122hypothetical protein
Bpet4825-129-7.369044disrupted lipopolysaccharides biosynthesis
Bpet4826043-10.071107ISxcc1 transposase
Bpet4827045-10.471423transposase
Bpet4828150-12.168831disrupted lipopolysaccharides biosynthesis
Bpet4829256-13.564487glycosyltransferase
Bpet4830254-12.539859hypothetical protein
Bpet4831147-10.333792putative UDP-N-acetylglucosamine 2-epimerase
Bpet4832038-8.249309glycosyltransferase
Bpet4833034-7.191087hypothetical protein
Bpet4834-222-3.509551hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4784INTIMIN290.034 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 28.9 bits (64), Expect = 0.034
Identities = 11/58 (18%), Positives = 25/58 (43%)

Query: 155 QLDLPDAPPGLPRSVQVSAVAISNPHAVQQVDDVDAAPVAAIGPLIERHPRFARRVNA 212
L++P G RS Q + + + + + ++ D+A + G + + A+ A
Sbjct: 455 SLNIPHDINGTERSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQDYQA 512


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4790adhesinb2485e-83 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 248 bits (635), Expect = 5e-83
Identities = 83/334 (24%), Positives = 157/334 (47%), Gaps = 42/334 (12%)

Query: 13 ILMMLALGTACASAPAMAR-AAEPLRVVATFSVLGDMVREIGGPDVAVTTLVGPDGDAHE 71
+L+ AC+S + + L VVAT S++ D+ + I G + + ++V D HE
Sbjct: 10 LLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSIVPVGQDPHE 69

Query: 72 YEPTPQAARQLAGAAVLIENGLSFET----WLPRLVKASGFAGRA--VVASQGIAPRKLA 125
YEP P+ ++ + A ++ NG++ ET W +LV+ + S+G+ L
Sbjct: 70 YEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVENAKKKENKDYYAVSEGVDVIYLE 129

Query: 126 DAEHEHDHGDDHDHDAGHEHGHGDADGHAHGHGTGHRHHHGDLDPHAWQSLTNGAVYARN 185
+ DPHAW +L NG +YA+N
Sbjct: 130 GQSEKGKE-----------------------------------DPHAWLNLENGIIYAQN 154

Query: 186 IGAGLAAADPEHAEAYRERAQAYIARIEALDARIRATFAAIPAARRQVVTSHDAFGYFGD 245
I L+ DP + E Y + +AY+ ++ ALD + F IP ++ +VTS F YF
Sbjct: 155 IAKRLSEKDPANKETYEKNLKAYVEKLSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSK 214

Query: 246 AYGVRFIAVAGFSTDAEPSAADMARIVEQVKRERVPAVFVENITSPALVRQIARETGARV 305
AY V + +T+ E + + +VE++++ +VP++FVE+ ++ ++++T +
Sbjct: 215 AYNVPSAYIWEINTEEEGTPDQIKTLVEKLRKTKVPSLFVESSVDDRPMKTVSKDTNIPI 274

Query: 306 GGTLYSDALAPSGKPAATYLGMFEWNARQLSAAL 339
+++D++A G+ +Y M ++N +++ L
Sbjct: 275 YAKIFTDSVAEKGEEGDSYYSMMKYNLEKIAEGL 308


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4800NUCEPIMERASE1696e-52 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 169 bits (429), Expect = 6e-52
Identities = 83/353 (23%), Positives = 132/353 (37%), Gaps = 46/353 (13%)

Query: 1 MSILVTGGAGFIGSNFVLAWLGGSDEPVINLDKLTYAGHAGNLD----SLQGDARHQLVH 56
M LVTG AGFIG + L + V+ +D L + +L L Q
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQ-VVGIDNLN-DYYDVSLKQARLELLAQPGFQFHK 58

Query: 57 GDIADNALVAALLQAHQPRAVLNFAAESHVDRAIRGPDAFIHTNVTGTFQLLEAVRAYWQ 116
D+AD + L + V V ++ P A+ +N+TG +LE R
Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118

Query: 117 ALGEPARTGFRYLQVSTDEVYGSLGPQDPPFAEGDPY-RPNNPYSASKAAGDHLVRAYWH 175
L S+ VYG + PF+ D P + Y+A+K A + + Y H
Sbjct: 119 Q---------HLLYASSSSVYGL--NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH 167

Query: 176 TYGLPVLTTHCPNNYGPRQFPEKLIPLLIHHALAGRPLPLYGDGSHVRDWLHVDDHCAGL 235
YGLP YGP P+ + L G+ + +Y G RD+ ++DD +
Sbjct: 168 LYGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAI 227

Query: 236 RRVLEAGE------------------PGQVYHVGAGQERSNLQVAQAVCALLDAWRPRAD 277
R+ + P +VY++G S +++ + AL DA A
Sbjct: 228 IRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNS---SPVELMDYIQALEDALGIEAK 284

Query: 278 GRAHGEQITFVQDRPGHDRRYAIDAGKIRRQLGWQPAHAFDAGLRATVQWYLD 330
+ +PG + D + +G+ P G++ V WY D
Sbjct: 285 -------KNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRD 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4801NUCEPIMERASE581e-11 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 57.9 bits (140), Expect = 1e-11
Identities = 46/201 (22%), Positives = 76/201 (37%), Gaps = 37/201 (18%)

Query: 21 MKILLLGATGQIGNALRRTLLPLG-------SITA-------PSRAQ-----------AD 55
MK L+ GA G IG + + LL G ++ +R + D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 56 LANLDGLRALLQAQVPDLIVNAAACTAVDQAENDPAPARRVNAEAVAVLAAHARKSG-AL 114
LA+ +G+ L + + + + AV + +P N + R +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 115 LVHYSTDYVFDGAKQTPYLETD-APHPLNEYGRSKLAGE-QAIAAS---GCRALVLRTSW 169
L++ S+ V+ ++ P+ D HP++ Y +K A E A S G A LR
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFT 180

Query: 170 VYAAHGR------NFVKTVLQ 184
VY GR F K +L+
Sbjct: 181 VYGPWGRPDMALFKFTKAMLE 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4807BLACTAMASEA320.003 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 32.1 bits (73), Expect = 0.003
Identities = 33/154 (21%), Positives = 53/154 (34%), Gaps = 12/154 (7%)

Query: 75 IDASSGQVLAAANPDMQIEPASLTKIMTAYVVFNALKEKRITLEQQVPVSEHAWRTGGSR 134
+D +SG+ L A D + S K++ V + LE+++ +
Sbjct: 45 MDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPV 104

Query: 135 MFIEPRKPVTVDELIQGMIVQSGNDASVALAEAVGGSESAFAALMNQEAERLGLKRTHF- 193
+TV EL I S N A+ L VGG + ++G T
Sbjct: 105 SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAG-----LTAFLRQIGDNVTRLD 159

Query: 194 -----MNATGLPDPQHVTTVADLA-VLSAALIND 221
+N D + TT A +A L L +
Sbjct: 160 RWETELNEALPGDARDTTTPASMAATLRKLLTSQ 193


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4810PF03309893e-23 Bvg accessory factor
		>PF03309#Bvg accessory factor

Length = 271

Score = 89.4 bits (222), Expect = 3e-23
Identities = 43/210 (20%), Positives = 74/210 (35%), Gaps = 25/210 (11%)

Query: 1 MILLIDSGNSRLKVGWLDNGAREPAAVAFDNL--DPHALGDWLGTLSRKPTLALGVNVAG 58
M+L ID N+ VG + V + +P D L +G +
Sbjct: 1 MLLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELALTIDG---LIGDDAER 57

Query: 59 AERG----------EGIRAALAGHGCPVHWITSRPQL-LGLRNGYTQPAQLGADRLVSLL 107
+R L + V + P + G+ P ++GADR+V+ L
Sbjct: 58 LTGASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCL 117

Query: 108 GVRSRLAQTHPPFVLASFGTATTIDTVGPDNAFAGGLILPGPALMRSSLARGTANLPLAD 167
+ ++ FG++ +D V F GG I PG + + A +A L +
Sbjct: 118 AAYHKYGT---AAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVE 174

Query: 168 ----GPVVDFPVDTHQAIASGIAAAQAGAV 193
V+ +T + + +G AG V
Sbjct: 175 LTRPRSVIGK--NTVECMQAGAVFGFAGLV 202


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4814PF04183280.047 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 28.3 bits (63), Expect = 0.047
Identities = 15/65 (23%), Positives = 25/65 (38%), Gaps = 6/65 (9%)

Query: 25 HIGAIEKHQDRAELVEICDTNPAALKAAHEATGARPFESLSDLLAESTADAVVLATPSGL 84
H A+++ ++ CD + A + F S + E+ D L P +
Sbjct: 167 HWLAVKREH----MIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPLP--V 220

Query: 85 HPWQA 89
HPWQ
Sbjct: 221 HPWQW 225


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4816HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4821ACRIFLAVINRP320.005 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 32.1 bits (73), Expect = 0.005
Identities = 12/62 (19%), Positives = 25/62 (40%), Gaps = 1/62 (1%)

Query: 291 LYDQIAKREVPALLGAADIGYISL-KPERLFRFGVSPNKLFDYMLARLPVLFAVRAGNNP 349
+ D +A L +G + L + R + + L Y L + V+ ++ N+
Sbjct: 154 ISDYVASNVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQ 213

Query: 350 VA 351
+A
Sbjct: 214 IA 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4825NUCEPIMERASE371e-04 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 37.1 bits (86), Expect = 1e-04
Identities = 21/129 (16%), Positives = 42/129 (32%), Gaps = 4/129 (3%)

Query: 289 NIMITGAGGSIGSELCRQILELRPRRMVLFEISEPALYTIEQELTTLRNTAGSEVEIIGV 348
++TGA G IG + +++LE ++V + Y + L R ++
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLE-AGHQVVGIDNLNDY-Y--DVSLKQARLELLAQPGFQFH 57

Query: 349 LGSVRDYEHCLLQLRRHGIQTVYHAAAYKHVPIVEHNIAEGILTNTFGTHAMARAAIKVG 408
+ D E + V+ + V N +N G +
Sbjct: 58 KIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK 117

Query: 409 VQDFVLIST 417
+Q + S+
Sbjct: 118 IQHLLYASS 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4826PF08280270.008 M protein trans-acting positive regulator
		>PF08280#M protein trans-acting positive regulator

Length = 530

Score = 26.7 bits (59), Expect = 0.008
Identities = 9/31 (29%), Positives = 17/31 (54%)

Query: 1 MLKEGETGVPVADLCRKHGISNATYYQWKSK 31
++K G P+ D R H +SN++ Y+ +
Sbjct: 128 LIKNGSHSRPLTDFARSHFLSNSSAYRMREA 158


72Bpet0089Bpet0100N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0089-1142.126837peptide chain release factor 3
Bpet0090-1113.434109hypothetical protein
Bpet0091-2113.375260putative ATP-dependent RNA helicase
Bpet0092-292.165894putative ATP-dependent RNA helicase
Bpet0093-182.586900LemA protein
Bpet0094-183.319249hypothetical protein
Bpet0095092.746294sensor histidine kinase
Bpet0096-1111.714957two-component response regulator
Bpet00970121.324840hypothetical protein
Bpet00981120.593245hypothetical protein
Bpet0099-113-0.260968putative flavocytochrome
Bpet0100-214-0.912746imidazolonepropionase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0089TCRTETOQM2249e-68 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 224 bits (572), Expect = 9e-68
Identities = 112/463 (24%), Positives = 203/463 (43%), Gaps = 54/463 (11%)

Query: 9 RRRTFAIISHPDAGKTTLTEKLLLFAGAIQIAGSVKARKASRHASSDWMEIEKQRGISVA 68
+ +++H DAGKTTLTE LL +GAI GSV +D +E+QRGI++
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTR----TDNTLLERQRGITIQ 57

Query: 69 SSVMQMEYRDCVINLLDTPGHQDFSEDTYRVLTAVDAALMVIDAANGVEPQTIRLLQVCR 128
+ + ++ + +N++DTPGH DF + YR L+ +D A+++I A +GV+ QT L R
Sbjct: 58 TGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 129 ARNTPIITFINKMDREVREPLDLLSEIEGHLGMDAVPFSWPVGMGKSFGGVFDIRRDRMR 188
P I FINK+D+ + + +I+ L + V V + M
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVI-KQKVELYP-----------NMC 165

Query: 189 VFRPGQERRSD-----DDDIIDG-LDNPEIASRFGSAFEQANGEIELIQEAAPAFDREAF 242
V + + D +DD+++ + + A E E
Sbjct: 166 VTNFTESEQWDTVIEGNDDLLEKYMSGKSL-----EALELEQEESIRFHN---------- 210

Query: 243 LAGRQTPVFFGSAINNFGVQEVLDALVEQAPPPGPRQALERLVEPQEPKFTGVVFKVQAN 302
PV+ GSA NN G+ +++ + + R + + G VFK++
Sbjct: 211 --CSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHR---------GQSELCGKVFKIE-- 257

Query: 303 MDPAHRDRVAFVRVSSGRFERGMRLKVARTNKEMRPNNVVSFLSQRRELLDEAYAGDVIG 362
R R+A++R+ SG ++++ K + + + ++ +D+AY+G+ I
Sbjct: 258 YSEK-RQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYTSINGELCKIDKAYSGE-IV 314

Query: 363 IPNHGVLQLGDVLTEGESLRFTGLPFFAPELFQ-AVEVKDPLRTKQLRIGLTQLGEEGAI 421
I + L+L VL + + L L Q VE P + + L L ++ + +
Sbjct: 315 ILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPL 374

Query: 422 QVFRPEAAGGTLLLGAVGQLQFEVVAHRLKTEYGVEARMLPSR 464
+ ++A ++L +G++Q EV L+ +Y VE +
Sbjct: 375 LRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPT 417


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0094cloacin392e-05 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 38.5 bits (89), Expect = 2e-05
Identities = 19/32 (59%), Positives = 20/32 (62%)

Query: 232 GGGGFGGGFGGGGGFGGGGGFGGGGGRSGGGG 263
GG G G +GGG G G GGG G GG SG GG
Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGG 79



Score = 36.2 bits (83), Expect = 1e-04
Identities = 17/37 (45%), Positives = 20/37 (54%)

Query: 229 GGRGGGGFGGGFGGGGGFGGGGGFGGGGGRSGGGGAS 265
GG G G G G G G GGG G GGG +GG ++
Sbjct: 47 GGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83



Score = 34.7 bits (79), Expect = 4e-04
Identities = 17/37 (45%), Positives = 17/37 (45%)

Query: 229 GGRGGGGFGGGFGGGGGFGGGGGFGGGGGRSGGGGAS 265
GG G G GG G G GG G GGG G G A
Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAV 84



Score = 34.3 bits (78), Expect = 4e-04
Identities = 17/30 (56%), Positives = 18/30 (60%)

Query: 237 GGGFGGGGGFGGGGGFGGGGGRSGGGGASG 266
GGG G G +GGG G G GGG GG SG
Sbjct: 47 GGGSGSGIHWGGGSGHGNGGGNGNSGGGSG 76



Score = 33.1 bits (75), Expect = 0.001
Identities = 21/51 (41%), Positives = 22/51 (43%), Gaps = 13/51 (25%)

Query: 229 GGRGGGGFGGG-------------FGGGGGFGGGGGFGGGGGRSGGGGASG 266
GG G G GGG +GGG G G G G G G GG G SG
Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSG 72



Score = 32.8 bits (74), Expect = 0.001
Identities = 19/34 (55%), Positives = 20/34 (58%), Gaps = 2/34 (5%)

Query: 233 GGGFGGGFGGGGGFGGGGGFGGGGGRSGGGGASG 266
GGG G G GGG G G G GG G SGGG +G
Sbjct: 47 GGGSGSGIHWGGGSGHGNG--GGNGNSGGGSGTG 78



Score = 30.8 bits (69), Expect = 0.006
Identities = 16/34 (47%), Positives = 17/34 (50%)

Query: 226 SRRGGRGGGGFGGGFGGGGGFGGGGGFGGGGGRS 259
S G GGG G G GGG G GGG GG +
Sbjct: 50 SGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83



Score = 30.5 bits (68), Expect = 0.007
Identities = 15/37 (40%), Positives = 15/37 (40%)

Query: 230 GRGGGGFGGGFGGGGGFGGGGGFGGGGGRSGGGGASG 266
GGG G GG G GGG G GG A G
Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91



Score = 30.5 bits (68), Expect = 0.007
Identities = 13/36 (36%), Positives = 15/36 (41%)

Query: 230 GRGGGGFGGGFGGGGGFGGGGGFGGGGGRSGGGGAS 265
G G GG G GG G G G G G + A+
Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0095PF06580330.002 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 33.3 bits (76), Expect = 0.002
Identities = 17/87 (19%), Positives = 35/87 (40%), Gaps = 19/87 (21%)

Query: 377 RVLVTAERDSDGAGRLAIHVDDDGAGIAATERERIFQRGVRMDEQRPGSGLGLDIVRD-L 435
++L+ +D G + + V++ G + + + +G GL VR+ L
Sbjct: 280 KILLKGTKD---NGTVTLEVENTG--------------SLALKNTKESTGTGLQNVRERL 322

Query: 436 ACTYGGDVQAG-ASPLGGLRITLLLPA 461
YG + Q + G + +L+P
Sbjct: 323 QMLYGTEAQIKLSEKQGKVNAMVLIPG 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0096HTHFIS861e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 85.7 bits (212), Expect = 1e-21
Identities = 36/120 (30%), Positives = 62/120 (51%), Gaps = 1/120 (0%)

Query: 2 RVLLIEDEPTLAAQLEQALRAAGYTVDRAADGQTAHYLGDVEAFDAVVLDLGLPVLDGLT 61
+L+ +D+ + L QAL AGY V ++ T D VV D+ +P +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLRRWRAAGRNMPVLILTARDSWHEKVAGIDAGADDYLAKPFHMEELLARV-RALIRRNS 120
+L R + A ++PVL+++A++++ + + GA DYL KPF + EL+ + RAL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0100TETREPRESSOR290.038 Tetracycline repressor protein signature.
		>TETREPRESSOR#Tetracycline repressor protein signature.

Length = 218

Score = 28.7 bits (64), Expect = 0.038
Identities = 14/53 (26%), Positives = 25/53 (47%), Gaps = 2/53 (3%)

Query: 308 YRAGGRIAMGTDAGTPFNRHGRNAEELAYMVAFGMTPADALVAGTSRAHELLG 360
YR G ++ +GT ++ +L +M G + D L A ++ +H LG
Sbjct: 93 YRDGAKVHLGTRPDEK--QYDTVETQLRFMTENGFSLRDGLYAISAVSHFTLG 143


73Bpet0190Bpet0194N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0190020-3.283741autotransporter
Bpet0191022-4.155599rod shape-determining protein MreB
Bpet0192026-4.666787hypothetical protein
Bpet0193026-4.557845autotransporter
Bpet0194335-8.318516transposase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0190PERTACTIN423e-05 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 41.6 bits (97), Expect = 3e-05
Identities = 99/477 (20%), Positives = 154/477 (32%), Gaps = 79/477 (16%)

Query: 350 SVQTSGEGSHGAY---AHDGGTIVLNGDSLSAGGNHAYGMYAKDPGSAIDATNVTVNTEG 406
S+ +GE HG + + G G ++ G A G+ ++P + + N +V + G
Sbjct: 40 SIIKAGERQHGIHIKQSDGAGVRTATGTTIKVSGRQAQGVLLENPAAELRFQNGSVTSSG 99

Query: 407 LYGFGARAENGGAITLKGGSISTDNATGQGTQDGDGSRAYALSADGANSSISAQDGVVIS 466
G +T+K G + D+AT D AL G + S D +
Sbjct: 100 QLFDEGVRRFLGTVTVKAGKLVADHATLANVSDTRDDDGIALYVAGEQAQASIADSTLQG 159

Query: 467 TKGQRA-YGAYAT--------NGGHI---------ELGGGSVTTQGFMAYGLYASGNGST 508
G R GA T G HI +L V + ASG +
Sbjct: 160 AGGVRVERGANVTVQRSTIVDGGLHIGTLQPLQPEDLPPSRVVLGDTSVTAVPASGAPAA 219

Query: 509 VDANGVDITT------SGGVGDGVWAYQGGTVNLNGGSVTVNGEPNANSPHETANGLVAV 562
V G + T +GG GV A G V+L ++ P A G V
Sbjct: 220 VFVFGANELTVDGGHITGGRAAGVAAMDGAIVHLQRATIRRGDAP--------AGGAVPG 271

Query: 563 GGTGSAAAGTINASDLSIVTRGANSAGAKAGATVDTDNTYGVINLERSTITVQGQAAVAA 622
G A G D + ++L +S + A A
Sbjct: 272 GAVPGGA--------------VPGGFGPLLDGWYGVDVSDSTVDLAQSIVEAPQLGA-AI 316

Query: 623 EINYGSTLTATDSTLVSEQGDGIVLNDNASVSLASTRVEAAGASLVSNLNAAGQTQNITV 682
G+ +T + +L + G+ I A R AS +S AG
Sbjct: 317 RAGRGARVTVSGGSLSAPHGNVIETGGGA-------RRFPPPASPLSITLQAGAR----- 364

Query: 683 GSGSNLTQNNGTLLQVNRGQEGMDGIVNLTLAAGSSSSGDVVDLDGLDQDSGLRDGGGKT 742
G L V LTLA G+ GD+V + G
Sbjct: 365 AQGRALLYRVLPE------------PVKLTLAGGAQGQGDIVATELPPIPGAS---SGPL 409

Query: 743 NFTVAQGASWIGIVRGINDLAAEDGGEIINVGGEPIAGNVTGGQDSTIVFQNGADIG 799
+ +A A W G R ++ L+ ++ ++ G + D ++ FQ A+ G
Sbjct: 410 DVALASQARWTGATRAVDSLSIDNATWVMT--DNSNVGALRLASDGSVDFQQPAEAG 464


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0191SHAPEPROTEIN488e-177 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 488 bits (1258), Expect = e-177
Identities = 246/347 (70%), Positives = 291/347 (83%), Gaps = 1/347 (0%)

Query: 1 MIGFMRSYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHEGGPNGKKIIQAVGHEA 60
M+ R FS DL+IDLGTANTLIYV+G+GIVL+EPSVVAIR + + K + AVGH+A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVA-AVGHDA 59

Query: 61 KQMLGRVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHPRNMLAPSPRIIVCVPCGST 120
KQMLGR PGNI AIRPMKDGVIADF VTE+ML+ FI+ VH + + PSPR++VCVP G+T
Sbjct: 60 KQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGAT 119

Query: 121 QVERRAIRESALGAGASHVYLIEEPMAAAIGAGLAVSDASGSMVVDIGGGTTEVAVISLG 180
QVERRAIRESA GAGA V+LIEEPMAAAIGAGL VS+A+GSMVVDIGGGTTEVAVISL
Sbjct: 120 QVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN 179

Query: 181 GMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEPTAELIKKEIGSAFPGSEVREIEVKGR 240
G+VY SVR+GGD+FDEAI+NY+RRNYG LIGE TAE IK EIGSA+PG EVREIEV+GR
Sbjct: 180 GVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGR 239

Query: 241 NLAEGVPRSFTVSSNEILESLTDPLNQIVSAVKIALEQTPPELGADITDKGIALTGGGAL 300
NLAEGVPR FT++SNEILE+L +PL IVSAV +ALEQ PPEL +DI+++G+ LTGGGAL
Sbjct: 240 NLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL 299

Query: 301 LRDLDRLLQEETGLPVVVAEESLTCVVRGCGQALDQLERLGEIFLRD 347
LR+LDRLL EETG+PVVVAE+ LTCV RG G+AL+ ++ G +
Sbjct: 300 LRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSE 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0193PRTACTNFAMLY445e-06 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 43.9 bits (103), Expect = 5e-06
Identities = 94/459 (20%), Positives = 156/459 (33%), Gaps = 75/459 (16%)

Query: 215 GASASVDRSRILTRGRDADGVSVK---HGGIAVVSKSSIQTSGRSADGISVSGE------ 265
A A + I+ G G+ ++ GG+ S ++I+ SGR A GI +
Sbjct: 31 AAHADWNNQSIVKTGERQHGIHIQGSDPGGVRTASGTTIKVSGRQAQGILLENPAAELQF 90

Query: 266 RSLVVGDSLKISTKGENSHGVDVEDGGTLLLSRGDVESKGKDGRGVAIDDGGRAVIVGSK 325
R+ V S ++S G V K G+ VA + +
Sbjct: 91 RNGSVTSSGQLSDDGIRRFLGTVTV---------------KAGKLVA-----DHATLANV 130

Query: 326 VSASGDRGIALHVDDKDSQAIVIGSRLQSSGRSGQAVLIEDGADALIAGSTIVADGLGV- 384
D GIAL+V + +QA + S LQ +G V IE GA+ + S IV GL +
Sbjct: 131 GDTWDDDGIALYVAGEQAQASIADSTLQGAG----GVQIERGANVTVQRSAIVDGGLHIG 186

Query: 385 QVQGKGSQLIGLDVDIDAESEARYRQSREPALGLRVEDQAKALLVGGSIQA--------- 435
+Q + + + ++ + + V ++ L GG I
Sbjct: 187 ALQSLQPEDLPPSRVVLRDTNVTAVPASGAPAAVSVLGASELTLDGGHITGGRAAGVAAM 246

Query: 436 -------------KGDQATGVSVGGSGSLVFAAG-----TDIRATGDDSTGMRVSRDARA 477
+GD G +V G A D G+ VS +
Sbjct: 247 QGAVVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPGGFGPVLDGWYGVDVS-GSSV 305

Query: 478 ILVGSDIQGGGKGLDI--TKGGQAGTVGGTVTATDARGVALSVSGRDSVAATIGTNLTAD 535
L S ++ G I +G + GG+++A + +G A L+
Sbjct: 306 ELAQSIVEAPELGAAIRVGRGARVTVSGGSLSAPHG---NVIETGGARRFAPQAAPLSIT 362

Query: 536 GQDGVAVEVRDAGRAYLIDSSLNARAVGLRAEGKDA--SVVSVGSSITAGTEIVPVGRAY 593
Q G G+A L + L G DA +V+ GT I P+ A
Sbjct: 363 LQAG----AHAQGKALLYRVLPEPVKLTL-TGGADAQGDIVATELPSIPGTSIGPLDVAL 417

Query: 594 SEPAVGV-ASDTGATVILIGGDVTALGDNSVAAQATSGD 631
+ A A+ ++ + +++V A + D
Sbjct: 418 ASQARWTGATRAVDSLSIDNATWVMTDNSNVGALRLASD 456


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0194HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


74Bpet0427Bpet0434N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0427-3130.936863rod shape-determining protein MreB
Bpet0428-2141.647273rod shape-determining protein MreC
Bpet0429-2140.932007rod shape-determining protein MreD
Bpet0430-3140.092254penicillin-binding protein
Bpet0431-215-1.320381rod shape-determining protein
Bpet0432-117-0.258787nucleoid occlusion protein
Bpet0433-118-0.298958putative hydrolase
Bpet0434-1190.086037acetylglutamate kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0427SHAPEPROTEIN5000.0 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 500 bits (1289), Expect = 0.0
Identities = 248/347 (71%), Positives = 293/347 (84%), Gaps = 1/347 (0%)

Query: 1 MFGFLRSYFSSDMAIDLGTANTLIYVRGKGIVLDEPSVVAIRHEGGPNGKKIIQAVGHEA 60
M R FS+D++IDLGTANTLIYV+G+GIVL+EPSVVAIR + + K + AVGH+A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVA-AVGHDA 59

Query: 61 KQMLGRVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHPRNMLAPSPRIIVCVPCGST 120
KQMLGR PGNI AIRPMKDGVIADF VTE+ML+ FI+ VH + + PSPR++VCVP G+T
Sbjct: 60 KQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGAT 119

Query: 121 QVERRAIRESALGAGASHVYLIEEPMAAAIGAGLAVSDASGSMVVDIGGGTTEVAVISLG 180
QVERRAIRESA GAGA V+LIEEPMAAAIGAGL VS+A+GSMVVDIGGGTTEVAVISL
Sbjct: 120 QVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN 179

Query: 181 GMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEPTAELIKKEIGSAFPGSEVREIEVKGR 240
G+VY SVR+GGD+FDEAI+NY+RRNYG LIGE TAE IK EIGSA+PG EVREIEV+GR
Sbjct: 180 GVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGR 239

Query: 241 NLAEGVPRSFTVSSNEILESLTDPLNQIVSAVKIALEQTPPELGADITDKGIALTGGGAL 300
NLAEGVPR FT++SNEILE+L +PL IVSAV +ALEQ PPEL +DI+++G+ LTGGGAL
Sbjct: 240 NLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL 299

Query: 301 LRDLDRLLQEETGLPVVVAEDPLTCVVRGCGEALEHLEKLGAIFIND 347
LR+LDRLL EETG+PVVVAEDPLTCV RG G+ALE ++ G ++
Sbjct: 300 LRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSE 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0430TONBPROTEIN372e-04 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 36.5 bits (84), Expect = 2e-04
Identities = 15/67 (22%), Positives = 19/67 (28%), Gaps = 1/67 (1%)

Query: 619 PIPPAADSSEDAPATSGPAPAATAAPVPVTAAPPAPEPAPRPRPAPRATPAPKPPAMPSR 678
+ P P P P P AP P+P+P P+ P K P R
Sbjct: 54 DLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAP-VVIEKPKPKPKPKPKPVKKVQEQPKR 112

Query: 679 RAPSTAP 685

Sbjct: 113 DVKPVES 119



Score = 36.1 bits (83), Expect = 3e-04
Identities = 21/89 (23%), Positives = 28/89 (31%), Gaps = 1/89 (1%)

Query: 616 PVGPIPPAADSSEDAPATSGPAPAATAAPVPVTAAPPAPEPAPRPRPAPRATPAPK-PPA 674
P PP E P APV + P P+P P+P + P P
Sbjct: 58 PQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPV 117

Query: 675 MPSRRAPSTAPALSDLTDLFQSPATGDKP 703
+P A + LT + AT
Sbjct: 118 ESRPASPFENTAPARLTSSTATAATSKPV 146



Score = 31.9 bits (72), Expect = 0.007
Identities = 18/54 (33%), Positives = 20/54 (37%), Gaps = 7/54 (12%)

Query: 630 APATSGPAPAATAAPVPVTAAPPAPEP-------APRPRPAPRATPAPKPPAMP 676
PA P A P PV P PEP AP P+ P PKP +
Sbjct: 51 TPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVK 104



Score = 30.7 bits (69), Expect = 0.015
Identities = 16/50 (32%), Positives = 21/50 (42%)

Query: 636 PAPAATAAPVPVTAAPPAPEPAPRPRPAPRATPAPKPPAMPSRRAPSTAP 685
PAPA + VT A P A +P P P P P+P +P +
Sbjct: 39 PAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVV 88


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0432HTHTETR513e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 51.2 bits (122), Expect = 3e-10
Identities = 30/181 (16%), Positives = 62/181 (34%), Gaps = 10/181 (5%)

Query: 1 MASKPGERKTQILQTLAEMLEQPHAARITTAALAARMQVSEAALYRHFASKAQMFEGLIE 60
+ E + IL + Q + + +A V+ A+Y HF K+ +F + E
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 61 FIEVSLFTLVNQIAAAEPHGLGQARKTVSMLLAFSERNKGMTRVLTGDALVTEDNRLQER 120
E ++ L + A+ G + ++ R L + + + + E
Sbjct: 65 LSESNIGELELEY-QAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEM 123

Query: 121 ------INHINDRIEASLKQSLRVAVSDGDLAPDANISAHASLLTHLVLG---RWLRYAQ 171
++ ++Q+L+ + L D A ++ + G WL Q
Sbjct: 124 AVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQ 183

Query: 172 S 172
S
Sbjct: 184 S 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0434CARBMTKINASE391e-05 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 39.0 bits (91), Expect = 1e-05
Identities = 27/129 (20%), Positives = 53/129 (41%), Gaps = 23/129 (17%)

Query: 154 EPIDIGFVGDITQVEPAVVKALQDDQFIPVISPIGYG----EDGTAYN-----INADVVA 204
+P VE +K L + I VI+ G G + I+ D+
Sbjct: 169 DPKGH--------VEAETIKKLVERGVI-VIASGGGGVPVILEDGEIKGVEAVIDKDLAG 219

Query: 205 GKMAEVLGAEKLLMMTNTPGVLDKSGK----LLRSLSAQTIDELFADGT-ISGGMLPKIA 259
K+AE + A+ +++T+ G G LR + + + + + +G +G M PK+
Sbjct: 220 EKLAEEVNADIFMILTDVNGAALYYGTEKEQWLREVKVEELRKYYEEGHFKAGSMGPKVL 279

Query: 260 SSLDAAKNG 268
+++ + G
Sbjct: 280 AAIRFIEWG 288



Score = 36.0 bits (83), Expect = 1e-04
Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 10/56 (17%)

Query: 32 GKTIVVKYGGNAMTEERLQRSFAHDVVLLKLV----------GLNPVVVHGGGPQI 77
GK +V+ GGNA+ + + S+ + ++ G V+ HG GPQ+
Sbjct: 2 GKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQV 57


75Bpet0532Bpet0537N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet05321141.930278prepilin signal peptidase
Bpet05330152.161330dephospho-CoA kinase
Bpet05340142.304714hypothetical protein
Bpet05350152.473020HlyD family secretion protein
Bpet05360162.328326AcrB/AcrD/AcrF family protein
Bpet0537-1142.668792AcrB/AcrD/AcrF family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0532PREPILNPTASE2376e-80 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 237 bits (607), Expect = 6e-80
Identities = 124/270 (45%), Positives = 157/270 (58%), Gaps = 1/270 (0%)

Query: 5 FAVDPGWAIAMAALLGLVVGSWLSVPAHRLPRMMEREWLQQYQEFRPAASGPEPAASAYT 64
P ++ L L++GS+L+V HRLP M+EREW +Y+ + Y
Sbjct: 8 AHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGVDEP-PYN 66

Query: 65 LWRPGWHCPACAAPVRGWRRLPVLGWLLLRGRCGACGEAIGWRYPAVEVTAALLFALCAW 124
L P CP C P+ +P+L WL LRGRC C I RYP VE+ ALL A
Sbjct: 67 LMVPRSCCPHCNHPITALENIPLLSWLWLRGRCRGCQAPISARYPLVELLTALLSVAVAM 126

Query: 125 RFGPTPIALCAMGLCAALLALAWIDLQTSLLPDAITLPLAWAGLLVNLGGALAPLPLAVL 184
P L A+ L L+AL +IDL LLPD +TLPL W GLL NL G L AV+
Sbjct: 127 TLAPGWGTLAALLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVI 186

Query: 185 GAVVGYVFLWLLFHMFRLLTGREGMGYGDFKLLAALGAWFGLAALPGLLLVASLAGVAGA 244
GA+ GY+ LW L+ F+LLTG+EGMGYGDFKLLAALGAW G ALP +LL++SL G
Sbjct: 187 GAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMG 246

Query: 245 GILRLTGHARRGQPLPFGPYLALAGMVMLL 274
L L + + +P+PFGPYLA+AG + LL
Sbjct: 247 IGLILLRNHHQSKPIPFGPYLAIAGWIALL 276


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0535RTXTOXIND448e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.0 bits (104), Expect = 8e-07
Identities = 24/194 (12%), Positives = 53/194 (27%), Gaps = 37/194 (19%)

Query: 8 TPTPRRKRRLAAIVLLLLAAAVAAWLLFKPGGSQQAATRGGRGFGGAATMNMPVPVRVAE 67
TP RR R +A ++ L A +
Sbjct: 51 TPVSRRPRLVAYFIMGFLVIAFI-LSVLGQ------------------------------ 79

Query: 68 AGTQDINIVLRALGTVTAY-NTVTVRSRVDGELVRVAFAEGQRVKAGDLLAQIDPRPFEV 126
+ IV A G +T + ++ + + + EG+ V+ GD+L ++ E
Sbjct: 80 -----VEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 127 ALAQAQGQQQQNQALLANARRDLQRYQTLFKQDSIARQQLDTQAALVRQYEGTQKIDQAA 186
+ Q Q + + + + + + Q + + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 187 VDNAKLQLSYTRIT 200
+ Q +
Sbjct: 195 FSTWQNQKYQKELN 208


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0536ACRIFLAVINRP8440.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 844 bits (2181), Expect = 0.0
Identities = 289/1036 (27%), Positives = 506/1036 (48%), Gaps = 29/1036 (2%)

Query: 4 SRLFILRPVATTLSMVAILIAGFIAYRLLPVSALPEVDYPTIQVVTLYPGASPDVMTSLV 63
+ FI RP+ + + +++AG +A LPV+ P + P + V YPGA + V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 64 TSPLERQFGQMPGLNQMSSTS-SGGASVITLQFSLDLSLDVAEQEVQAAINAASNLLPSD 122
T +E+ + L MSSTS S G+ ITL F D+A+ +VQ + A+ LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 123 LPVPPIYNKVNPADAPVLTLAISS--PTMPLPQVRDLVDTRMAQKLSQVPGVGLVGVAGG 180
+ I + + ++ S P + D V + + LS++ GVG V + G
Sbjct: 122 VQQQGIS-VEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QRPAVRIQVNPRALAAAGMSLADLRTAVVGANVNQPKGNLDGP------ERSTTIDANDQ 234
Q A+RI ++ L ++ D+ + N G L G + + +I A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 235 LKSPTDYNDLII-AYRNNAPLRLSDVATAVQGAEDVRQAAWAGGQPAILLNVQRQPGANV 293
K+P ++ + + + + +RL DVA G E+ A G+PA L ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 IDVVDRIRAMLPQAQAALPATLDVSIVSDRTQTIRASVSDVQFEMMLAVALVVMVTFLFL 353
+D I+A L + Q P + V D T ++ S+ +V + A+ LV +V +LFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 RSLTATFIPSVVVPLSLVGTFGIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENIARH 413
+++ AT IP++ VP+ L+GTF I+ G+SIN LT+ + +A G +VDDAIV++EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 L-EQGETPMQAALKGAAQIGFTLISLTFSLIAVLIPLLFMTEVVGRLFREFAITLAVAIL 472
+ E P +A K +QI L+ + L AV IP+ F G ++R+F+IT+ A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 ISLVVSLTLTPMMCARLLRPESEQRH---GRFHQATGAFIDRTIAHYDRMLQWVLAHQRL 529
+S++V+L LTP +CA LL+P S + H G F D ++ HY + +L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 530 TLLVALGTFVLTALLYIAIPKGFFPQQDTGMIQAITQAPASVSFPAMAQRQQEAARIVLQ 589
LL+ +L++ +P F P++D G+ + Q PA + + + L+
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 590 D--PDVESVSSFIGVDGTNATLNTGRMQIALKPHGERDGD---LAEVTRRLQQALDAQQG 644
+ +VESV + G + N G ++LKP ER+GD V R + L +
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 645 LKVYMQPVQDLTIEDRVSRTQYQMTL---SNPDIAVLAEWAPKLVERLSQLP-ELTDVAH 700
V P I + + T + L + L + +L+ +Q P L V
Sbjct: 660 GFVI--PFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRP 717

Query: 701 DLQDDGLQTWVDIDRDAAARLGISTSAIDEALYNAFGQRLISTIFTQSNQYRVVLEVLPQ 760
+ +D Q +++D++ A LG+S S I++ + A G ++ + ++ ++ +
Sbjct: 718 NGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAK 777

Query: 761 FRQSPEALGQIHLATESGTLVPLSAVAHISQGRTMLAINRLDQFPMTTVSFNLAPGASLS 820
FR PE + ++++ + +G +VP SA + R + P + APG S
Sbjct: 778 FRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG 837

Query: 821 AAVDAIAEAEAGIGMPASIETRYQGAALAFQNSLSSTLWLILAAVITMYIVLGVLYESYI 880
A+ + + +PA I + G + + S + L+ + + +++ L LYES+
Sbjct: 838 DAMALMENLASK--LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWS 895

Query: 881 HPVTILSTLPSAAVGALLALMISGTELDMIGIIGIILLIGIVKKNAIMMIDFALEAERKR 940
PV+++ +P VG LLA + + D+ ++G++ IG+ KNAI++++FA + K
Sbjct: 896 IPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKE 955

Query: 941 GLAPRAAIHEAALLRFRPILMTTLAALFGALPLMLSTGTGAELRQPLGLVMVGGLLLSQV 1000
G A A +R RPILMT+LA + G LPL +S G G+ + +G+ ++GG++ + +
Sbjct: 956 GKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATL 1015

Query: 1001 LTLFTTPVIYLMFDRL 1016
L +F PV +++ R
Sbjct: 1016 LAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0537ACRIFLAVINRP7910.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 791 bits (2045), Expect = 0.0
Identities = 291/1032 (28%), Positives = 504/1032 (48%), Gaps = 28/1032 (2%)

Query: 7 FIVRPVATVLLCLGLVLAGVLSFRLLPVAPLPEVDLPIISVTANLPGASPETMASSVATP 66
FI RP+ +L + L++AG L+ LPVA P + P +SV+AN PGA +T+ +V
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 67 LERSLGSIAGVTEMTSR-NSQGSTRITLQFDLSRDIDGAARDVQAAINAARSLLPTGLRS 125
+E+++ I + M+S +S GS ITL F D D A VQ + A LLP ++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ- 123

Query: 126 NPTYHKVNPSSAPIMVLALTSDT--LSQGRLYDLASTIVAQKLAQVNGVGEVTVGGSSLP 183
SS+ +MV SD +Q + D ++ V L+++NGVG+V + G+
Sbjct: 124 QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY- 182

Query: 184 AVRVNLIPGALSSRGVSLDEVRATLTEANANRPKGVVENDRY------HWQIMASDQLER 237
A+R+ L L+ ++ +V L N G + + I+A + +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKN 242

Query: 238 AEQYRPLVV-AWRDGAAVRLSDVATVEDSVEDLFQTGFYNNRQAILLILRRQADANIIET 296
E++ + + DG+ VRL DVA VE E+ N + A L ++ AN ++T
Sbjct: 243 PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDT 302

Query: 297 VEAIRAQLPQLAALLPGDVDMTVAQDRTPSIRASLHEAELTLVVAVALVMLVVLLFLRHW 356
+AI+A+L +L P + + D TP ++ S+HE TL A+ LV LV+ LFL++
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 357 RAALIPSVAVPVSLVGTFCIMYLCGYTLNTISLMALIVATGFVVDDAIVVLENIMRHI-E 415
RA LIP++AVPV L+GTF I+ GY++NT+++ +++A G +VDDAIVV+EN+ R + E
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 416 QGASPMRAALRGSREVGFTVLSMSLSLVAVFIPILLMGGVVGRLFREFAVTLSAAIMVSL 475
P A + ++ ++ +++ L AVFIP+ GG G ++R+F++T+ +A+ +S+
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 476 VVSLTLTPMMCARLLR--TQDHGRAPGRLSRAIGRGFDAVLARYRRSLSWALAHGRIMLL 533
+V+L LTP +CA LL+ + +H G FD + Y S+ L LL
Sbjct: 483 LVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLL 542

Query: 534 LLAAAIGLNVYLYAVVPKGFFPQQDTGQLLGFFRVDQGTSFQATVPKLEYFRKVILSDP- 592
+ A + V L+ +P F P++D G L ++ G + + T L+ L +
Sbjct: 543 IYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEK 602

Query: 593 ----AVASITVHAGGRGGSNSSFMSIQLKPQAERKASANDV---VNRLRGRLQNTPGARV 645
+V ++ + N+ + LKP ER N ++R + L V
Sbjct: 603 ANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFV 662

Query: 646 FLVPQQDIFLGGGQGSGSYDYTLLAGELSL-LRTWMPKV-QQAMAALPELTDVDTSVEDK 703
I G ++ AG L ++ A L V + +
Sbjct: 663 IPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLED 722

Query: 704 GRQVELVIDREAATRLGISMSDISAVLNNSFSQRQVSVMYGPLNQYHVVMGVVQRFAQDA 763
Q +L +D+E A LG+S+SDI+ ++ + V+ + + +F
Sbjct: 723 TAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLP 782

Query: 764 ESLKQVHVITQDGRRVPLAAFAHFESGNAPLSVRHNGLLAADEISFNLAPGVSLDQAIRA 823
E + +++V + +G VP +AF + L + EI APG S A+
Sbjct: 783 EDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMAL 842

Query: 824 IDAAVARIGLPSDQIQAGFLGTAAAQQEVQSQQPWLILGALVTMYIVLGILYENLVHPLT 883
++ ++ LP+ I + G + ++ +Q P L+ + V +++ L LYE+ P++
Sbjct: 843 MENLASK--LPAG-IGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 884 ILSTLPSAGIGALLALMLVGSEFTIIALIGVFLLIGIVKKNAIMMVDFALDAERRRGLSP 943
++ +P +G LLA L + + ++G+ IG+ KNAI++V+FA D + G
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGV 959

Query: 944 RDAIFEACLTRFRPIMMTTLAAIFGALPLVLATGAGVEMRQPLGVTIVGGLILSQILTLY 1003
+A A R RPI+MT+LA I G LPL ++ GAG + +G+ ++GG++ + +L ++
Sbjct: 960 VEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIF 1019

Query: 1004 TTPVVYLYLDRF 1015
PV ++ + R
Sbjct: 1020 FVPVFFVVIRRC 1031



Score = 91.8 bits (228), Expect = 8e-21
Identities = 75/502 (14%), Positives = 166/502 (33%), Gaps = 29/502 (5%)

Query: 5 APFIVRPVATVLLCLGLVLAGVLSFRLLPVAPLPEVDLPIISVTANLPGASPETMASSVA 64
+ +L+ +V V+ F LP + LPE D + LP + + V
Sbjct: 531 GKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVL 590

Query: 65 TPLER-----------SLGSIAGVTEMTSRNSQGSTRITLQFDLSRDIDGAARDVQAAIN 113
+ S+ ++ G + + G ++L+ + +G +A I+
Sbjct: 591 DQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLK--PWEERNGDENSAEAVIH 648

Query: 114 AARSLLPTGLRSNPTYHKVNPSSAPIMVLALTSDTLSQG-----RLYDLASTIVAQKLAQ 168
A+ L + + + Q L + ++
Sbjct: 649 RAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQH 708

Query: 169 VNGVGEVTVGGSS-LPAVRVNLIPGALSSRGVSLDEVRATLTEANANRPKGVVEND-RYH 226
+ V G ++ + + GVSL ++ T++ A + R
Sbjct: 709 PASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVK 768

Query: 227 WQIMASDQLER--AEQYRPLVVAWRDGAAVRLSDVATVEDSVEDLFQTGFYNNRQAILLI 284
+ +D R E L V +G V S T + + + +
Sbjct: 769 KLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWV----YGSPRLERYNGLPSM 824

Query: 285 LRRQADANIIETVEAIRAQLPQLAALLPGDVDMTVAQDRTPSIRASLHEAELTLVVAVAL 344
+ A + +A A + LA+ LP + + R S ++A + ++ +
Sbjct: 825 EIQGEAAPGTSSGDA-MALMENLASKLPAGIGYDWT-GMSYQERLSGNQAPALVAISFVV 882

Query: 345 VMLVVLLFLRHWRAALIPSVAVPVSLVGTFCIMYLCGYTLNTISLMALIVATGFVVDDAI 404
V L + W + + VP+ +VG L + ++ L+ G +AI
Sbjct: 883 VFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAI 942

Query: 405 VVLENIM-RHIEQGASPMRAALRGSREVGFTVLSMSLSLVAVFIPILLMGGVVGRLFREF 463
+++E ++G + A L R +L SL+ + +P+ + G
Sbjct: 943 LIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAV 1002

Query: 464 AVTLSAAIMVSLVVSLTLTPMM 485
+ + ++ + ++++ P+
Sbjct: 1003 GIGVMGGMVSATLLAIFFVPVF 1024


76Bpet0653Bpet0672N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0653-1161.235866putative hydrolase
Bpet06543150.879923hypothetical protein
Bpet06553150.643886DMT superfamily permease
Bpet0656216-0.207325putative periplasmic protein
Bpet0657215-0.737198peptidoglycan-associated lipoprotein
Bpet0658114-0.544838translocation protein TolB
Bpet0659215-0.816791colicin exporter
Bpet0660-415-0.934212biopolymer transport protein TolR
Bpet0661-4110.285436TolQ colicin import protein
Bpet0662-3100.517412hypothetical protein
Bpet0663-3110.880834prolyl-tRNA synthetase
Bpet0664-4101.425507dinucleoside polyphosphate hydrolase
Bpet0665-3151.507940two component response regulator
Bpet0666-2150.834403two-component system histidine kinase
Bpet0667-1170.129652LysR family transcriptional regulator
Bpet0668-116-0.489385hypothetical protein
Bpet0669-113-0.338997putative oxidoreductase
Bpet0670-113-0.997740hypothetical protein
Bpet0671-114-0.846345hypothetical protein
Bpet06720150.197846hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0653PF06057280.043 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 27.9 bits (62), Expect = 0.043
Identities = 40/190 (21%), Positives = 61/190 (32%), Gaps = 43/190 (22%)

Query: 60 SLRHYWPQQWDGVDFSMDQHVDDLLAFIDTVGE--GAAHV--VGHSRGARVALEAALRAP 115
SL++YW Q+ D LA ID G V +G+S GA V P
Sbjct: 86 SLKYYWKQK------DPKDVTQDTLAIIDKYQAEFGTQKVILIGYSFGAEVIPFVLNEMP 139

Query: 116 ARVRSLTLADPGLPMPGREGD------TRGGFRQRALALIEAGEVDAGLALFVDTVSGAD 169
AR R + L P + D ++ + EV+ + + + G +
Sbjct: 140 ARYRK-NVLGAVLLSPSQSSDFEIHVSEMVTSDNQSARYLTLPEVNKQTTVPMLCLYGKE 198

Query: 170 TWRRMVPWFKDMVRDNASTLGGQATEHL-PPVPREQVERLALPTLLIGGALSPAPYPAVL 228
A HL P V + V + L GG Y V+
Sbjct: 199 D---------------------DAPLHLCPEVKQPNVTVMELS----GGHSFDDDYDKVV 233

Query: 229 DMLAEWLPAA 238
++ WL +
Sbjct: 234 KLIKGWLKPS 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0657OMPADOMAIN928e-25 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 91.9 bits (228), Expect = 8e-25
Identities = 27/114 (23%), Positives = 47/114 (41%), Gaps = 11/114 (9%)

Query: 62 SVYFDFDSYTVPDQYRGLVETHARYLASH--QQQRVQIQGNTDERGGAEYNLALGQRRAD 119
V F+F+ T+ + + ++ L++ + V + G TD G YN L +RRA
Sbjct: 220 DVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQ 279

Query: 120 AVRRMMTLLGVSDAQIETISFGKEKPRATGTTE---------ADFAENRRADIE 164
+V + G+ +I G+ P T + A +RR +IE
Sbjct: 280 SVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIE 333


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0659IGASERPTASE692e-14 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 68.6 bits (167), Expect = 2e-14
Identities = 36/205 (17%), Positives = 61/205 (29%), Gaps = 25/205 (12%)

Query: 67 PPPDVQPDEPKPEPEPPKPQPQPEPAPQPEPQPQPEPPPPPPPPVEKPQPPQPDPEIALE 126
++ P P E A P PPP P P E +
Sbjct: 993 DTTNITTPNNIQADVPSVPSNNEEIA---RVDEAPVPPPAPATPSETTE---------TV 1040

Query: 127 EARKKREEEEKARQEAEAAKEKARLEEERKQAELKEKQRLEQERKAAEKAAAEKAAAEKA 186
K+E + + E +A + A+ ++ + K ++ + E A + E
Sbjct: 1041 AENSKQESKTVEKNEQDATETTAQ----NREVAKEAKSNVKANTQTNEVAQSGSETKETQ 1096

Query: 187 AAEKAAAEKAAAEKAAAEKAAAEKAAAEKKAKEEAAKKAAAEKAAAEKAAAEKAAAEKAA 246
E K A EKA E + +E K + E++ + AE A
Sbjct: 1097 TTET---------KETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAR 1147

Query: 247 AEKAAAEKKAKEEAAKKAAAAKKAA 271
K + A ++ A
Sbjct: 1148 ENDPTVNIKEPQSQTNTTADTEQPA 1172



Score = 56.6 bits (136), Expect = 1e-10
Identities = 38/226 (16%), Positives = 62/226 (27%), Gaps = 24/226 (10%)

Query: 62 DTPDSPPPDV----QPDEPKPEPEPPKPQPQPEPAPQP-EPQPQPEPPPPPPPPVEK--- 113
DT + P+ P P E + P P P P P E K
Sbjct: 993 DTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVE 1052

Query: 114 -----PQPPQPDPEIALEEARKK----REEEEKARQEAEA-------AKEKARLEEERKQ 157
+EA+ + E A+ +E KE A +E+E K
Sbjct: 1053 KNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKA 1112

Query: 158 AELKEKQRLEQERKAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKKA 217
EK + + + E++ + AE A + A E+ A
Sbjct: 1113 KVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPA 1172

Query: 218 KEEAAKKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKKAKEEAAKK 263
KE ++ + A + E++ K
Sbjct: 1173 KETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNK 1218



Score = 52.8 bits (126), Expect = 2e-09
Identities = 28/149 (18%), Positives = 42/149 (28%), Gaps = 5/149 (3%)

Query: 134 EEEKARQEAEAAKEKARLEEERKQAELKEKQRLEQERKAAEKAAAEKAAAEKAAAEKAAA 193
E EK Q + + E+ + E A A + + A
Sbjct: 984 EVEKRNQTVDT--TNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVA 1041

Query: 194 EKAAAEKAAAEKA---AAEKAAAEKKAKEEAAKKAAAEKAAAEKAAAEKAAAEKAAAEKA 250
E + E EK A E A ++ +EA A E A + E E
Sbjct: 1042 ENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETK 1101

Query: 251 AAEKKAKEEAAKKAAAAKKAAADKALREA 279
KEE AK + + +
Sbjct: 1102 ETATVEKEEKAKVETEKTQEVPKVTSQVS 1130



Score = 46.6 bits (110), Expect = 2e-07
Identities = 26/176 (14%), Positives = 49/176 (27%), Gaps = 23/176 (13%)

Query: 69 PDVQPDEPKPEPEPPKPQPQPEPAPQPEP------------------QPQPEPPPPPPPP 110
P V + + QPQ EPA + +P QP E P
Sbjct: 1123 PKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQP 1182

Query: 111 VEKPQPPQPDPEIALEEARKKREEEEKARQEAEAAKEKARLEEERKQAELKEKQRLEQER 170
V + + + E A + E + + R + ++ +
Sbjct: 1183 VTESTTVNTGNSVV-----ENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPA 1237

Query: 171 KAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKKAKEEAAKKAA 226
+ + A + + A A KA KA ++ ++ E +
Sbjct: 1238 TTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQHISQLEMNNEGQ 1293



Score = 44.7 bits (105), Expect = 5e-07
Identities = 26/220 (11%), Positives = 60/220 (27%), Gaps = 13/220 (5%)

Query: 72 QPDEPKPEPEPPKPQPQPEPAPQPEPQPQPEPPPPPPPPVEKPQPP---QPDPEIALEEA 128
+ PK + Q Q E +PQ +P P +++PQ D E +E
Sbjct: 1119 TQEVPKVTSQVSPKQEQSETV---QPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKET 1175

Query: 129 RKKREEEEKARQEAEAAKEKARLEEERKQAELKEKQRLEQERKAAEKA--AAEKAAAEKA 186
E+ E A + E K + +
Sbjct: 1176 SSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVE 1235

Query: 187 AAEKAAAEKAAAEKAAAEKAAAEKAAAEKKAKEEAAKKAAAEKAAAEKAAAEKAAAEKAA 246
A ++ +++ ++ +AK + + + + E +
Sbjct: 1236 PATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQHISQLEMNNEGQYN 1295

Query: 247 AEKA-AAEKKAKEEAAKKAAAAKKAAA----DKALREAFR 281
+ + K + + ++K D+ + +
Sbjct: 1296 VWVSNTSMNKNYSSSQYRRFSSKSTQTQLGWDQTISNNVQ 1335



Score = 38.9 bits (90), Expect = 3e-05
Identities = 31/210 (14%), Positives = 58/210 (27%), Gaps = 8/210 (3%)

Query: 59 AEGDTPDSPPPDVQPDEPKPEPEPPKPQPQPEPAPQPEP-QPQPEPPPPPPPPVEKPQPP 117
E T ++ E K + E K Q P+ Q P Q Q E P P
Sbjct: 1093 KETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEP-----AR 1147

Query: 118 QPDPEIALEEARKKREEEEKARQEAEAAKEKARLEEERKQAELKEKQRLEQERKAAEKAA 177
+ DP + ++E + + Q A+ +E
Sbjct: 1148 ENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATT 1207

Query: 178 AEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKAAAEKKAKEEAAKKAAAEKAAAEKAAA 237
+E + K ++ + A ++ ++ + A A
Sbjct: 1208 QPTVNSESSNKPKNRHRRSVRSVPHNVEPAT--TSSNDRSTVALCDLTSTNTNAVLSDAR 1265

Query: 238 EKAAAEKAAAEKAAAEKKAKEEAAKKAAAA 267
KA KA ++ ++ E +
Sbjct: 1266 AKAQFVALNVGKAVSQHISQLEMNNEGQYN 1295


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0660FLGMRINGFLIF290.009 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 28.8 bits (64), Expect = 0.009
Identities = 18/120 (15%), Positives = 45/120 (37%), Gaps = 25/120 (20%)

Query: 20 VVPYIDVMLVLLVIFMVTAPLITPGLINLPSVGQASEVPATPLEVQISEDGQVALRMREA 79
++ +LVL+V +++ + P L E A + Q+ ++ +
Sbjct: 458 LLAAGRWLLVLVVAWILWRKAVRPQLTRR-----VEEAKAAQEQAQVRQETEE------- 505

Query: 80 GATPQDIARDQLVDQVRQRITAETPVVIAADGKVPYETVVKVMDELRSNGVTRLGLLVDQ 139
A +++D+ + Q R ++ E + + + E+ N + L++ Q
Sbjct: 506 -AVEVRLSKDEQLQQRRANQ------------RLGAEVMSQRIREMSDNDPRVVALVIRQ 552


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0665HTHFIS906e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.5 bits (222), Expect = 6e-23
Identities = 35/143 (24%), Positives = 66/143 (46%), Gaps = 2/143 (1%)

Query: 2 RILLVEDELEMASWLVRALAQSGFTPDHAPDARSAEALMAANEYDAIVMDLRLPDKHGLV 61
IL+ +D+ + + L +AL+++G+ +A + +AA + D +V D+ +PD++
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLRDMRGRDDRTPVLLLTAQGALQDRVRGLNLGADDFLTKPFALEELEARVAALVRRSRG 121
+L ++ PVL+++AQ ++ GA D+L KPF L EL + + +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 122 RQFPRLQCGSLAYD--GESRAFT 142
R G S A
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQ 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0666PF06580330.002 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.002
Identities = 9/40 (22%), Positives = 15/40 (37%), Gaps = 4/40 (10%)

Query: 393 LVHNAIHYA----PAGARITVSVARRGSRAEVAVSDNGPG 428
LV N I + P G +I + + + V + G
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSL 302


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0670TATBPROTEIN300.009 Bacterial sec-independent translocation TatB protein...
		>TATBPROTEIN#Bacterial sec-independent translocation TatB protein

signature.
Length = 171

Score = 30.4 bits (68), Expect = 0.009
Identities = 13/65 (20%), Positives = 26/65 (40%), Gaps = 7/65 (10%)

Query: 140 FKFGPAEYFSLMTLGLVGAVVLASGSLPKAIAMIILGLLLGMVGTDVNS--GVARYDFGI 197
F G + L+ + ++G VVL LP A+ + + + + + + +
Sbjct: 2 FDIG---FSELLLVFIIGLVVLGPQRLPVAVKTV--AGWIRALRSLATTVQNELTQELKL 56

Query: 198 PELQD 202
E QD
Sbjct: 57 QEFQD 61


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0671ACRIFLAVINRP280.022 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 27.9 bits (62), Expect = 0.022
Identities = 17/90 (18%), Positives = 34/90 (37%), Gaps = 15/90 (16%)

Query: 37 MGPGYFPFALGLVLAILGAIV--------LLGSMTKSATETHVDK-------FDWRIAFL 81
G Y F++ +V A+ +++ L ++ K + H + F+
Sbjct: 463 TGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHS 522

Query: 82 VIGSVILYGLILKLLGIYISVFVLVVVSSL 111
V G IL G Y+ ++ L+V +
Sbjct: 523 VNHYTNSVGKILGSTGRYLLIYALIVAGMV 552


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0672RTXTOXIND280.012 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.3 bits (63), Expect = 0.012
Identities = 19/143 (13%), Positives = 44/143 (30%), Gaps = 17/143 (11%)

Query: 3 QDLDQLAARIGQLVQRTRQLHAERDALRVRLSQSESSQRALEQRCADHQA---------- 52
+ + Q + AER + R+++ E+ R + R D +
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKH 252

Query: 53 EIQALQAKLQEHDSAVAGMLNEARQTESDLREQLARATADRQSLES-------RASAREA 105
+ + K E + + ++ Q ES++ Q ++ + +
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 106 ELQGSLHARDTDLQRLRVAASAA 128
L L + Q + A +
Sbjct: 313 LLTLELAKNEERQQASVIRAPVS 335


77Bpet0893Bpet0903N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet0893-2184.012721septum formation inhibitor
Bpet0894-2153.744851glutaminyl-tRNA synthetase
Bpet0895-1164.154656putative transmembrane cytochrome oxidase
Bpet0896-3163.529672putative cytochrome oxidase
Bpet0897-2163.729590two-component sensor kinase
Bpet0898-2153.376808transcriptional regulator
Bpet0899-2132.911622putative secreted protein
Bpet0900-2162.824170DNA helicase
Bpet0901-2203.156559Holliday junction DNA helicase RuvB
Bpet0902-1183.163349serine/threonine dehydratase
Bpet0903-1173.181974NADH dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0893TONBPROTEIN300.012 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 29.6 bits (66), Expect = 0.012
Identities = 26/110 (23%), Positives = 31/110 (28%), Gaps = 5/110 (4%)

Query: 103 PPARPAPAVETAPPNDAATPVPAVPAAALETGASATTGNAPAEPAPAEPAAPAAAPQPPA 162
P V P P P E +P E APA A
Sbjct: 79 PEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTA 138

Query: 163 VPAPA----SALVITKPLRSGQRVY-ARHTDLVVIGMVSQGAEVIADGNV 207
A + S + L Q Y AR L + G V +V DG V
Sbjct: 139 TAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRV 188


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0897PF06580300.021 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.2 bits (68), Expect = 0.021
Identities = 17/107 (15%), Positives = 35/107 (32%), Gaps = 25/107 (23%)

Query: 392 LLDNLIGNALHHGEPP------VDVSLRREGGMAMLDVADHGRGIAPERRSEALRPFARL 445
L+ L+ N + HG + + ++ G L+V + G +
Sbjct: 259 LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKEST------- 311

Query: 446 DDARTRTGNVGLGLA-LAEAIARAHGGQLAL-LQADSGGLLVRITLP 490
G GL + E + +G + + L G + + +P
Sbjct: 312 ----------GTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0898HTHFIS1021e-27 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 102 bits (257), Expect = 1e-27
Identities = 52/148 (35%), Positives = 79/148 (53%), Gaps = 1/148 (0%)

Query: 6 TKLLVVDDDPALRQLLADYLNRHGYDTLLAPDANDLAARIARYAPDLLVLDRMLPGGDGA 65
+LV DDD A+R +L L+R GYD + +A L IA DL+V D ++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 DACRRLREQGEDIPVILLTARDEAVDRIIGLEAGADDYLGKPFDPRELLARIE-AVLRRK 124
D R+++ D+PV++++A++ + I E GA DYL KPFD EL+ I A+ K
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 125 RGPSALTKDAPVSFGPFVFDPAMRQLLR 152
R PS L D+ AM+++ R
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYR 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0899PF00577563e-10 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 55.6 bits (134), Expect = 3e-10
Identities = 36/236 (15%), Positives = 62/236 (26%), Gaps = 42/236 (17%)

Query: 268 GRLAYSSTVGVLNYTDMAARSGAIDYGVTAGSGTLRYGLTPELTLESQMQSAPDLSTRGL 327
G YS T G A TL +GL T+ Q A
Sbjct: 372 GHTRYSITAGEYR------SGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNF 425

Query: 328 GSTYSAGDLGTFQAGATQSSFD-----DINAWRYRFGYNVNLFE---SVSLAVTNEQIGA 379
G + G LG TQ++ + RF YN +L E ++ L +
Sbjct: 426 GIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLV-GYRYSTS 484

Query: 380 GFGDLAQY-------------------------RNGVAAAPQMRNTLAAGVPIMGWGTLT 414
G+ + A +A + + L + TL
Sbjct: 485 GYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLY 544

Query: 415 GTYSGLRQSGEPIEQR-FGLQHSMLIA-PSVRLAVGADRDVVTGDYEMRAGVTMPV 468
+ S G F + + L+ ++ + + + +
Sbjct: 545 LSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0900HTHFIS310.018 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.018
Identities = 17/78 (21%), Positives = 32/78 (41%), Gaps = 4/78 (5%)

Query: 158 LSMLHERWPDVPRIALTATATAATRVEIAQRLALDQARHFVASFDRPNIRYRIV-EKNEV 216
L + + PD+P + ++A T T ++ +++ A D FD + I E
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDY---LPKPFDLTELIGIIGRALAEP 122

Query: 217 RRQLLDLIRAEHEGDSGV 234
+R+ L +G V
Sbjct: 123 KRRPSKLEDDSQDGMPLV 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet0903NUCEPIMERASE512e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 51.3 bits (123), Expect = 2e-09
Identities = 27/132 (20%), Positives = 47/132 (35%), Gaps = 24/132 (18%)

Query: 1 MRILLIGGTGFLGRHMAARLAGHGHVLIV---------PTRQYGRGRDLQLL--PTLTLV 49
M+ L+ G GF+G H++ RL GH ++ + + R L+LL P
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQAR---LELLAQPGFQFH 57

Query: 50 EADVHDDAVLDRLLR--ECDAVINLAGILHGGRGQPYGAGFARVHVQLP----QRIAQAC 103
+ D+ D + L + V Y + I + C
Sbjct: 58 KIDLADREGMTDLFASGHFERVFISP----HRLAVRYSLENPHAYADSNLTGFLNILEGC 113

Query: 104 RRHGVRRLLHVS 115
R + ++ LL+ S
Sbjct: 114 RHNKIQHLLYAS 125


78Bpet1326Bpet1333N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1326341-8.176081acetyltransferase
Bpet1327440-8.029815putative oxidoreductase
Bpet1328338-7.122572asparagine synthetase, glutamine-hydrolyzing
Bpet1329238-6.626972MFS transporter
Bpet1330135-6.044777putative monooxygenase
Bpet1331238-7.7736662,4-dichlorophenol 6-monooxygenase
Bpet1332140-8.218728TetR family transcriptional regulator
Bpet1333134-7.268649major facilitator superfamily permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1326SACTRNSFRASE334e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 32.6 bits (74), Expect = 4e-04
Identities = 15/63 (23%), Positives = 32/63 (50%), Gaps = 5/63 (7%)

Query: 94 SGWCGW--VQSMVVSPSWRRMGIAESLMHELLQWFSLLGVTKVVLESTQV---AEAMYQK 148
S W G+ ++ + V+ +R+ G+ +L+H+ ++W ++LE+ + A Y K
Sbjct: 84 SNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAK 143

Query: 149 LGF 151
F
Sbjct: 144 HHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1329TCRTETB509e-09 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 49.9 bits (119), Expect = 9e-09
Identities = 79/375 (21%), Positives = 145/375 (38%), Gaps = 52/375 (13%)

Query: 43 LPMIETAFSVPVAIAAQLVTAFTLAYGLGSPIFVALLPAHQQRAGLLFALGLFVLANAAS 102
LP I F+ P A + TAF L + +G+ ++ L + LLF + + +
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 103 ALS-TDFTVLMVFRAIAGIGAGVYLAMGIAASAALSPPDQRGKSIAVIMGGMASGTVLGV 161
+ + F++L++ R I G GA + A+ + A P + RGK+ +I +A G +G
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 162 PLSLLLAEQLGWESALWLVTLLGAIAFVGLIARLPSLPTVQAIPLKAKIALLTDSHVVVI 221
+ ++A + W S L L+ ++ I L+ L ++ I L++ V +
Sbjct: 157 AIGGMIAHYIHW-SYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFM 215

Query: 222 LLVS----LLAAISSLGMYTFLAPLIAAAEPNSSP------------------------- 252
L + +S L F+ + +P P
Sbjct: 216 LFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGF 275

Query: 253 -SVTPY-----------------LWVWGVGGVLGSFLIGPLVDRVKGPTLTLWI-MAILA 293
S+ PY ++ + ++ ++ G LVDR +GP L I + L+
Sbjct: 276 VSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDR-RGPLYVLNIGVTFLS 334

Query: 294 VALLLLPASLSTGPWLVMLPIAIWGAVGWALQVPQNNELIKAREQQGDGNLAVALNESAL 353
V+ L L T W + + I + + + + +QQ G LN ++
Sbjct: 335 VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS- 393

Query: 354 YLGSALGAAMGGVLL 368
+L G A+ G LL
Sbjct: 394 FLSEGTGIAIVGGLL 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1332HTHTETR601e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 60.4 bits (146), Expect = 1e-13
Identities = 30/173 (17%), Positives = 69/173 (39%), Gaps = 5/173 (2%)

Query: 13 SRERGRPREFDIHTALDRAILYFREHGYNGVSIADLSQALKLSAGSIYKAFHSKHGLFTA 72
+++ + I LD A+ F + G + S+ ++++A ++ G+IY F K LF+
Sbjct: 5 TKQEAQETRQHI---LDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 73 ALDRYMALRGQQIAEITASAESG-REKLRRLLVFYAESSHAAEGRYGCLVVVGAVELSST 131
+ + G+ E A LR +L+ ES+ E R + ++
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121

Query: 132 DEAIAAKV-ASALSANERRLKAIIEQGQQDGSISRTAEPGTTAKLMLALLQGM 183
+ A+ + + + R++ ++ + + A +M + G+
Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1333TCRTETB393e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 38.7 bits (90), Expect = 3e-05
Identities = 64/372 (17%), Positives = 119/372 (31%), Gaps = 45/372 (12%)

Query: 47 ISAQLNLSEQASGLIVTLTQLGYGLGLLLVVPLGDLFENRRLAISILAVGAIGLLISGFA 106
I+ N ++ + T L + +G + L D +RL + + + G +I
Sbjct: 40 IANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVG 99

Query: 107 GSVEPFL-AASFLVGLGSVTVQILVPYA-AHLAPDATRGRVVGNVMSGLMLGIMLARPVA 164
S L A F+ G G+ LV A P RG+ G + S + +G + +
Sbjct: 100 HSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIG 159

Query: 165 SMITYFTSWRVVFFLSFIGMVLLAGVLRFALPTRPVVARLRY-HQMLASMA--------- 214
MI ++ W + + I ++ + +++ + +L S+
Sbjct: 160 GMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT 219

Query: 215 -------------------HLVRTT-----PALRRRALYHASMFGAFSLFWTTTPLLLAG 250
H+ + T P L + + + +F T +
Sbjct: 220 SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMV 279

Query: 251 PQ-----FGLS--QKGIALFALAGVAGAIAAPIAGRIADRGWIRSATAAAMLLGIGAFAI 303
P LS + G + ++ I I G + DR + +F
Sbjct: 280 PYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLT 339

Query: 304 TYIGDIGTSLSLAMFVIAGVVLDFAVSANLVLGQRVIFSLAPEIRGRLNGLYMTTFFCGG 363
TS + + ++ VL V+ V SL + G L T F
Sbjct: 340 ASFLLETTSWFMTIIIVF--VLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSE 397

Query: 364 AIGSALGGWLFA 375
G A+ G L +
Sbjct: 398 GTGIAIVGGLLS 409


79Bpet1431Bpet1436N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1431037-6.066999putative 4-hydroxybenzoate transporter
Bpet1432233-5.486374putative resolvase
Bpet1433132-5.573549putative transcriptional regulator
Bpet1434130-5.422493transcriptional regulator
Bpet1435127-4.249320TetR family transcriptional regulator
Bpet1436125-3.661775putative transmembrane efflux protein of the MFS
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1431TCRTETA516e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 50.6 bits (121), Expect = 6e-09
Identities = 70/380 (18%), Positives = 128/380 (33%), Gaps = 65/380 (17%)

Query: 53 LAPSIAENFGLEVGSFAPVFAAGLFGLMVGALLLGPIADKIGRRWLVIAATFTFGLFTFL 112
+ + ++G+ + +A A +LG ++D+ GRR +++ + + +
Sbjct: 37 HSNDVTAHYGILLALYA-------LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI 89

Query: 113 TASASSINEFVILRFLTGLGLGGAMPNLTALATEYSPR----RYQGMIVAWLFAGIPIGA 168
A+A + I R + G+ G A + + R+ G + A G+ G
Sbjct: 90 MATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGP 148

Query: 169 IVGGLLSSWLLPFAGWQAAFHVGGVLPMLLALVLVFTLPESLRFLILKQDNPRRVLAIAN 228
++GGL+ + A F L L L F LPES + + P R A+
Sbjct: 149 VLGGLMGGF-----SPHAPFFAAAALNGLNFLTGCFLLPESHK----GERRPLRREAL-- 197

Query: 229 RIVPSGFPPEQQFSSPQKPVTGIPVRHLFTNGRWSGTLLLWIPYFMNLLIIYFI---ISW 285
P+ T L+ ++FI +
Sbjct: 198 -----------------NPLASFRWARGMTVVAA-------------LMAVFFIMQLVGQ 227

Query: 286 LPAML-------RQSGMPITAGIEAATAFSFGGAIGCLGTGKLLQMFGARKVALIEFVAT 338
+PA L R T GI A + TG + G R+ ++ +A
Sbjct: 228 VPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIAD 287

Query: 339 ILFILLLSTYSDAYWSVMLIAGFLGFTVQGAQAALNALVAGFYPTAIRSTGIGWALGIGR 398
+LL+ + W I L AL A+++ + G +
Sbjct: 288 GTGYILLAFATR-GWMAFPIMVLLAS-GGIGMPALQAMLSRQVDEERQGQLQGSLAALTS 345

Query: 399 VGSIIGPLIGGLMLSMHWQT 418
+ SI+GPL+ + + T
Sbjct: 346 LTSIVGPLLFTAIYAASITT 365


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1432SUBTILISIN260.041 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 26.3 bits (58), Expect = 0.041
Identities = 15/60 (25%), Positives = 25/60 (41%), Gaps = 4/60 (6%)

Query: 8 DILIVTKLDRHGRDAI-DISTTVRTLAEMGVRVYCLALGGADLTSSAGTMTMNVLNAVAQ 66
D+LI+ L++ G I + E V + ++LGG + + V AVA
Sbjct: 111 DLLIIKVLNKQGSGQYDWIIQGIYYAIEQKVDIISMSLGGPE---DVPELHEAVKKAVAS 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1435HTHTETR733e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 73.1 bits (179), Expect = 3e-18
Identities = 33/160 (20%), Positives = 59/160 (36%), Gaps = 2/160 (1%)

Query: 12 AVIDAAMDVFWTNGFEASSTQELCERTGLGRGSLYHAFGSKQNLYEQALRRYQE-LGLKA 70
++D A+ +F G ++S E+ + G+ RG++Y F K +L+ + + +G
Sbjct: 15 HILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELE 74

Query: 71 QTEILNGPGTAKERLQALLQWGVDGDLDPEKRRSCMA-LFSVMERGSKDPVIDQINRAYV 129
PG L+ +L ++ + E+RR M +F E + V+ Q R
Sbjct: 75 LEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNLC 134

Query: 130 NRLEAVICHVIAVGQRNGELADDRPALEVARAFLASYYGL 169
I + L D A GL
Sbjct: 135 LESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1436TCRTETA392e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 39.4 bits (92), Expect = 2e-05
Identities = 82/368 (22%), Positives = 130/368 (35%), Gaps = 36/368 (9%)

Query: 37 VTQIGYLISLYAIGMVVGGPLLTVGLLKLRVPNKQALLWLLGFYAVAQSVAASATSYDIM 96
G L++LYA+ P+L G L R + LL L AV ++ A+A ++
Sbjct: 42 TAHYGILLALYALMQFACAPVL--GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVL 99

Query: 97 AAARVATGVAGSACFGVSLAICAEIVGAESR----GRAASIVVGGLMLATVLGVPIATII 152
R+ G+ G A V+ A A+I + R G ++ G++ VLG +
Sbjct: 100 YIGRIVAGITG-ATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGF- 157

Query: 153 DQHWGWRASFWLVVALAVLCATVITFLVPRSKAAGTVSLGAELAEFKNRHLWAAYATSGL 212
A F+ AL L FL+P S L E WA T
Sbjct: 158 ----SPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVA 213

Query: 213 IIGATFAAFSYFAPILTEV--------TGFAAASIPWLLGVYGAANVVGNMVVGRYADKH 264
+ A F + + + A +I L +G + + ++
Sbjct: 214 ALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAAR 273

Query: 265 --TMPIMVWGLIVLGAALAVFSIFAQNQVLSLGALIVIGLVGV--PMNPAMIARVMKTAH 320
++ G+I G + FA ++ ++++ G+ P AM++R +
Sbjct: 274 LGERRALMLGMIADGTGY-ILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEER 332

Query: 321 PGAL--VNTVHTSVINIGLGVGAWVGGLGIAAGYGNRSPLWVGVALAVLGLLSL--LPYL 376
G L TS+ +I VG L A Y W G A L L LP L
Sbjct: 333 QGQLQGSLAALTSLTSI-------VGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPAL 385

Query: 377 GRKAASRA 384
R S A
Sbjct: 386 RRGLWSGA 393


80Bpet1549Bpet1557N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1549-1120.701934ferric uptake regulation protein
Bpet1550-2121.834784DNA repair protein
Bpet1551-2130.636943NAD(+)/NADH kinase family protein
Bpet1552315-1.285157heat-inducible transcription repressor
Bpet1553415-1.073840ferrochelatase
Bpet1554618-1.583779hypothetical protein
Bpet1555415-0.502375heat shock protein GrpE
Bpet1556315-0.566341putative thioredoxin
Bpet1557215-0.792776molecular chaperone DnaK
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1549ACRIFLAVINRP280.017 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 27.9 bits (62), Expect = 0.017
Identities = 10/49 (20%), Positives = 21/49 (42%), Gaps = 5/49 (10%)

Query: 31 QRHLSAEDVYRALIGENVEIG----LATVYRVLTQFEQAGILSRSQFDS 75
+ L+ DV L +N +I T Q + I+++++F +
Sbjct: 195 KYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNAS-IIAQTRFKN 242


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1550CHANLCOLICIN320.008 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 32.0 bits (72), Expect = 0.008
Identities = 31/122 (25%), Positives = 51/122 (41%), Gaps = 4/122 (3%)

Query: 260 LQGVADELESARIAVSEAVSDL---NNYVSRVDLDPQRLAEVETRMSAVFETARKFKTEP 316
L+ + +E + + + ++L NN + + + RLA+ E + E A K E
Sbjct: 94 LKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEA 153

Query: 317 EALPALRESVQAQLAD-LQAAADIDALRARADAAAAQYETAAGKLSAARARVAKSLGKQV 375
E E +A+ L+ A + A A E A KLSAA++ V K G+
Sbjct: 154 EQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIK 213

Query: 376 TQ 377
T
Sbjct: 214 TL 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1551PF06057352e-04 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 35.2 bits (81), Expect = 2e-04
Identities = 23/102 (22%), Positives = 42/102 (41%), Gaps = 14/102 (13%)

Query: 69 DTASNTGVHEYPVATLQEIGATAS-----LAVVMGGDGTVL----GAARTLAPYGVPLVG 119
+ A N G+ PV ++ A +S L + + GDG L G P+VG
Sbjct: 24 EFADNLGLTLLPVEPSTQVNAASSHTKPPLVIFLSGDGGWATLDKAVGGILQQQGWPVVG 83

Query: 120 INHGRLGFITDVPLQEAHIALARVIEGNYQAE---DRMLLVG 158
+ + + ++ +I+ YQAE +++L+G
Sbjct: 84 WSSLKY-YWKQKDPKDVTQDTLAIID-KYQAEFGTQKVILIG 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1555IGASERPTASE290.010 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 29.3 bits (65), Expect = 0.010
Identities = 25/115 (21%), Positives = 46/115 (40%), Gaps = 6/115 (5%)

Query: 8 VDQAPESNEPAPAVPA-----TVEALQAELAAVRAELEAAQATVAGQQEQVLRARADAEN 62
VD+AP PAPA P+ E + E V + A T A +E A+++ +
Sbjct: 1020 VDEAPVP-PPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKA 1078

Query: 63 VRRRAQEDVSKARKFGIESFAESLVPVKDSLEAALAQPDQTLEALREGVEVTLKQ 117
+ + S + ++ + E A + ++T E + +V+ KQ
Sbjct: 1079 NTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQ 1133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1557SHAPEPROTEIN1414e-39 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 141 bits (356), Expect = 4e-39
Identities = 86/396 (21%), Positives = 142/396 (35%), Gaps = 93/396 (23%)

Query: 2 SKIIGIDLGTTNSCVAVMDGGQVKIIENAEGART----TPSIVAYMDDGETLVGAPAKRQ 57
S + IDLGT N+ + V G V + R +P VA VG AK+
Sbjct: 10 SNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVA-------AVGHDAKQM 62

Query: 58 AVTNPKNTLYAVKRLIGRKFDEKAVQKDIDLMPYSIVKADNGDAWVEARGKKIAPPQVSA 117
P N + A++ + + IA V+
Sbjct: 63 LGRTPGN-IAAIRPM---------------------------------KDGVIADFFVTE 88

Query: 118 DVLRK-MKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAA 176
+L+ +K+ + ++ VP +R+A +++ + AG +I EP AAA
Sbjct: 89 KMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAA 148

Query: 177 LAFGLDKSEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQR 236
+ GL SE V D+GGGT +V++I + V + +GG+ FD+
Sbjct: 149 IGAGLPVSE--ATGSMVVDIGGGTTEVAVISLNGV---------VYSSSVRIGGDRFDEA 197

Query: 237 IIDYIIGEFKKEQGVDLSKDVLALQRLKEAAEKAKIELSSS----QQTEINLPYITADAS 292
II+Y+ + G + AE+ K E+ S+ + EI +
Sbjct: 198 IINYVRRNYGSLIG-------------EATAERIKHEIGSAYPGDEVREIEVRGRNLAEG 244

Query: 293 GPKHLNLKITRAKLEALVEEL----------IERTIDPCRVAIKDAGVKVSEIDDVILVG 342
P+ L + LEAL E L +E+ I + G ++L G
Sbjct: 245 VPRGFTLN-SNEILEALQEPLTGIVSAVMVALEQCPPELASDISERG--------MVLTG 295

Query: 343 GMTRMPKVQEKVKEFFGKDPRKDVNPDEAVAAGAAI 378
G + + + E G +P VA G
Sbjct: 296 GGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGK 331


81Bpet1592Bpet1602N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet15922141.867630TetR family transcriptional regulator
Bpet15932141.859968multiple drug resistance protein MarC
Bpet1594-1132.232444putative chromate transport protein
Bpet1595-3130.751525sensor histidine kinase
Bpet1596-1130.551432two-component transcriptional regulator
Bpet15970141.079768MarR family transcriptional regulator
Bpet1598-1150.782557hypothetical protein
Bpet1599-2130.210397hypothetical protein
Bpet1600-115-0.122730*putative oxidoreductase
Bpet1601-216-0.393508hypothetical protein
Bpet1602-116-0.312331putative membrane transport protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1592HTHTETR535e-11 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 53.1 bits (127), Expect = 5e-11
Identities = 30/180 (16%), Positives = 64/180 (35%), Gaps = 3/180 (1%)

Query: 1 MARPREFDETAVLDAAVRCFWARGFEATSVRELAESMGITGASLYNAFGDKRSLYRRALD 60
+ + +LD A+R F +G +TS+ E+A++ G+T ++Y F DK L+ +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 61 HYVETSFSARARRVESLP--PSEALAAFFADIVERSLADRQRKGCMIINSALEVAPHD-P 117
P P L ++E ++ + +R+ M I +
Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMA 124

Query: 118 EFQQVVVSVLGRIEAFFLRCVTTGQQAGTIITTQPADDLARLLLSVLMGVRVMARARPER 177
QQ ++ + + +A + A ++ + G+ P+
Sbjct: 125 VVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQS 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1594BACINVASINB290.035 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 29.3 bits (65), Expect = 0.035
Identities = 30/101 (29%), Positives = 46/101 (45%), Gaps = 5/101 (4%)

Query: 81 GGYLGALAAWAGFTLPSAALLVLFALGISHFGQALAPGVLHGLKVVAVAVVAQAVWNMAR 140
GG ALAA G + A +V A G+S QAL P + H LK + + ++ +A+
Sbjct: 337 GGASLALAA-VGLAVMVADEIVKAATGVSFIQQALNPIMEHVLKPL-MELIGKAITKALE 394

Query: 141 SLCADARRA---GIMVAAACAASLLPYAWTQIGILAAAAVA 178
L D + A G +V A AA + + ++ A A
Sbjct: 395 GLGVDKKTAEMAGSIVGAIVAAIAMVAVIVVVAVVGKGAAA 435


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1595PF06580310.011 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.6 bits (69), Expect = 0.011
Identities = 19/103 (18%), Positives = 38/103 (36%), Gaps = 21/103 (20%)

Query: 361 IATLVDNALKYA----GDAARIRIETVQTDRETRLTVADNGPGIPAAKMDRIGERFYRAH 416
+ TLV+N +K+ +I ++ + + L V + G + E
Sbjct: 260 VQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSL----ALKNTKE------ 309

Query: 417 RHVPGYGLGLATVMA-IARLHGG--RLELSNAEPGLCVRLHFP 456
G GL V + L+G +++LS + + + P
Sbjct: 310 ----STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1596HTHFIS736e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 72.6 bits (178), Expect = 6e-17
Identities = 32/132 (24%), Positives = 53/132 (40%), Gaps = 1/132 (0%)

Query: 3 RCLIVEDDADNARYIANGLKELGYDPVITMDGPTALQRATTEHWDAIILDRMLPNDVDGL 62
L+ +DDA + L GYD IT + T + D ++ D ++P D +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMP-DENAF 63

Query: 63 SILWSLRALGRKTPVLVLSALTALDERVRGLKAGGDDYLTKPFAFPELAARVEALIRRSS 122
+L ++ PVLV+SA ++ + G DYL KPF EL + +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 123 SGYERRQLTVAD 134
+ + D
Sbjct: 124 RRPSKLEDDSQD 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1600DHBDHDRGNASE1132e-32 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 113 bits (283), Expect = 2e-32
Identities = 69/261 (26%), Positives = 114/261 (43%), Gaps = 12/261 (4%)

Query: 2 LKGKVAIVTGSTSGIGLGIATAFAQQGADIVLNGFGDAAEIEKLRAGLASQHGVKVLYDG 61
++GK+A +TG+ GIG +A A QGA I E +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIA--AVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 62 ADLSRGEAVRQLVANTVQSLGRVDILVNNAGIQHTALIEDFPVEKWDAILALNLSAVFHG 121
AD+ A+ ++ A + +G +DILVN AG+ LI E+W+A ++N + VF+
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 122 TAAALPHMKKQGWGRIINIASAHGLVGSANKSAYVAAKHGVVGLTKVTALETAGSGITAN 181
+ + +M + G I+ + S V + +AY ++K V TK LE A I N
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 182 AICPGWVRTGLVEKQITALAEKDHVDQ----DAAARKLLSEKQPSLQFVTPEQLGGTAVY 237
+ PG T + Q + A+++ +Q K P + P + ++
Sbjct: 184 IVSPGSTETDM---QWSLWADENGAEQVIKGSLETFKT---GIPLKKLAKPSDIADAVLF 237

Query: 238 LASDAAAQVTGTSISVDGGWT 258
L S A +T ++ VDGG T
Sbjct: 238 LVSGQAGHITMHNLCVDGGAT 258


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1602TCRTETB355e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.9 bits (80), Expect = 5e-04
Identities = 31/168 (18%), Positives = 64/168 (38%), Gaps = 13/168 (7%)

Query: 30 PYLTQELNLSAADLGSLTSLYFLGFALMQLPAGLFLDTWGPRRVNALMLLLAAAGTLVYG 89
P + + N A + + + L F++ G D G +R+ +++ G+++
Sbjct: 38 PDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGF 97

Query: 90 LSDS-LPGLMAGRLLIGAGVCVCLGAAFQAL-----AQTFPLARLPMVNGLVMAVGGLGG 143
+ S L+ R + GAG AAF AL A+ P GL+ ++ +G
Sbjct: 98 VGHSFFSLLIMARFIQGAG-----AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGE 152

Query: 144 VLVGSPLSWLLERSSWQSVSIGLAFFTLTVAALIWFGAPREGARHRST 191
+ + + W + + +TV L+ ++ R +
Sbjct: 153 GVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKL--LKKEVRIKGH 198


82Bpet1640Bpet1647N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1640-19-0.409399hypothetical protein
Bpet1641-110-0.475923hypothetical protein
Bpet1642-1120.738807hypothetical protein
Bpet1643-1121.170729hypothetical protein
Bpet1644-2130.500649amino acid transporter
Bpet1645-2121.954918putative secreted protein
Bpet1646-2112.589787putative beta-hydroxyacid dehydrogenase
Bpet1647-292.7806283-hydroxybutyrate dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1640cloacin351e-04 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 35.1 bits (80), Expect = 1e-04
Identities = 23/66 (34%), Positives = 28/66 (42%), Gaps = 5/66 (7%)

Query: 27 PDGKGKPAQAGHGNG-----NGNGSGNGSGNGKSGNSGGNPGNGNGGTKGSRGGDDINVS 81
P G G A G+G N G G+GSG G SG G GNG + G G +
Sbjct: 24 PTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83

Query: 82 VSLATA 87
V+ A
Sbjct: 84 VAAPVA 89



Score = 33.1 bits (75), Expect = 5e-04
Identities = 21/74 (28%), Positives = 27/74 (36%)

Query: 40 NGNGNGSGNGSGNGKSGNSGGNPGNGNGGTKGSRGGDDINVSVSLATAGISIAAARGYAA 99
G G+GSG G G +GG GN GG+ + V+ +S A G A
Sbjct: 46 WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAV 105

Query: 100 DYGLTGYSALPPGI 113
SA I
Sbjct: 106 SISAGALSAAIADI 119


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1641PF07201300.011 Hypersensitivity response secretion protein HrpJ
		>PF07201#Hypersensitivity response secretion protein HrpJ

Length = 293

Score = 29.8 bits (67), Expect = 0.011
Identities = 21/157 (13%), Positives = 39/157 (24%), Gaps = 25/157 (15%)

Query: 63 TEELSHLEVIGTLVAMLNKGAKGELAEATESEADLYRSLHGAGND-SHVTQVLYGGGPAL 121
EL + + L+++L+ L+ L L G + S ++L G
Sbjct: 94 VPELEQKQNVSELLSLLSNSPNISLS-------QLKAYLEGKSEEPSEQFKMLCG---LR 143

Query: 122 TNSGGQLWNAGYIDTIGDPSADLRSNIAAEARAKIVYERLINVTE--------DPGVKEA 173
G+ A + + +T +
Sbjct: 144 DALKGRPELAHLSHLVEQALVSMAEEQGETIVLGA------RITPEAYRESQSGVNPLQP 197

Query: 174 LGFLMTREVSHQRSFEKALYSMQPNFPPGKLPGDPRF 210
L V + +Q FP G + F
Sbjct: 198 LRDTYRDAVMGYQGIYAIWSDLQKRFPNGDIDSVILF 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1645TRNSINTIMINR300.014 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 29.7 bits (66), Expect = 0.014
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 12/72 (16%)

Query: 74 LTLGLVAAGAMLAAGSGMAQPQPTTPAPATQDAAAEQQLDSSDKDFLENAAQSGHAEVEG 133
+++G +AAG A +G+AQ TP P ++D D NAA+S +
Sbjct: 236 VSVGAIAAGLAGLAATGIAQALALTPEPDDP--------TTTDPDQAANAAESATKD--- 284

Query: 134 SKMAQEKAKNPD 145
++ QE KNP+
Sbjct: 285 -QLTQEAFKNPE 295


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1647DHBDHDRGNASE931e-24 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 92.8 bits (230), Expect = 1e-24
Identities = 75/264 (28%), Positives = 116/264 (43%), Gaps = 12/264 (4%)

Query: 4 AAELQGRCALVTGSTASLGLAIADRLAAAGARI--VLHNLLADEPARQARDALARRHGTD 61
A ++G+ A +TG+ +G A+A LA+ GA I V +N E + A AR H
Sbjct: 3 AKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEAR-HAEA 61

Query: 62 VLLQAADLNEVDQIEAMAHEARHAFGGIDIVVNNAVVRHFGPADTLPRNQWDEALAVNVS 121
D +D+I A G IDI+VN A V G +L +W+ +VN +
Sbjct: 62 FPADVRDSAAIDEITARIERE---MGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNST 118

Query: 122 AAFHLARLSIPGMRSRGWGRIINMSSVYGSGATANRVGYITTKTALIGLTRALAVETAQD 181
F+ +R M R G I+ + S + Y ++K A + T+ L +E A+
Sbjct: 119 GVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEY 178

Query: 182 GITCNAVAPGTVPTPTIASRIAGIARDQGISEQQAQHDYLAHRQ--PTGRFVDMANVAAL 239
I CN V+PG+ T S A D+ +EQ + + P + +++A
Sbjct: 179 NIRCNIVSPGSTETDMQWSLWA----DENGAEQVIKGSLETFKTGIPLKKLAKPSDIADA 234

Query: 240 IGFLCSDAGRDITGALLPIDGGWT 263
+ FL S IT L +DGG T
Sbjct: 235 VLFLVSGQAGHITMHNLCVDGGAT 258


83Bpet1695Bpet1704N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1695-113-0.3939902-C-methyl-D-erythritol 4-phosphate
Bpet16960110.1553642-C-methyl-D-erythritol 2,4-cyclodiphosphate
Bpet16972100.983629AhpD protein
Bpet1698-1111.191528alkyl hydroperoxide reductase
Bpet16991101.847416hypothetical protein
Bpet17001102.598177osmolarity response regulator
Bpet17011102.986705putative acetyltransferase
Bpet17022121.914298bacteriophage-related DNA polymerase
Bpet17031131.429041lysophospholipid transporter LplT
Bpet17043141.292326putative chromosome partition protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1695PRTACTNFAMLY290.014 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 29.3 bits (65), Expect = 0.014
Identities = 28/115 (24%), Positives = 39/115 (33%), Gaps = 5/115 (4%)

Query: 37 RWAVAALLADARIGQVRVAVSPGDERAGAALAGLPRTVCRPCGG--PTRAATVAAALADS 94
R A A + A + R + GD AG A+ G GG P V
Sbjct: 239 RAAGVAAMQGAVVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPGGFGPVLDGWYGV 298

Query: 95 NAADSDWILVHDAAR-PGLPPDALARLIDACLADAVGGLLALPVADTVKAGGPRV 148
+ + S L P L A R+ GG L+ P + ++ GG R
Sbjct: 299 DVSGSSVELAQSIVEAPEL--GAAIRVGRGARVTVSGGSLSAPHGNVIETGGARR 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1699PF06580401e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 40.2 bits (94), Expect = 1e-05
Identities = 18/104 (17%), Positives = 34/104 (32%), Gaps = 22/104 (21%)

Query: 351 LIENARRYG-RSTDGLAHLKMTLQAEGSILVIEVSDRGPGIAPEEVDRLLRPFSRGEAAR 409
L+EN ++G + + + + +EV + G +
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE------------- 309

Query: 410 TGVSGAGLGLAIVERLLKHVGG---SLKMLARPGGGLTARIEIP 450
G GL V L+ + G +K+ + G A + IP
Sbjct: 310 ----STGTGLQNVRERLQMLYGTEAQIKLSEKQGKV-NAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1700HTHFIS1024e-27 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 102 bits (255), Expect = 4e-27
Identities = 38/135 (28%), Positives = 69/135 (51%), Gaps = 1/135 (0%)

Query: 11 KILVVDDDPRLRDLLRRYLSEQGFNVFVAEDAKEMGKLWQREHFDLLVLDLMLPGEDGLS 70
ILV DDD +R +L + LS G++V + +A + + DL+V D+++P E+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 71 ICRRLRGGHDNTPIIMLTAKAEEIDRIVGLEMGADDYLSKPFNPRELLARI-NAILRRRG 129
+ R++ + P+++++A+ + I E GA DYL KPF+ EL+ I A+ +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 130 TEEHPGAPSQENESI 144
SQ+ +
Sbjct: 125 RPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1701SACTRNSFRASE472e-08 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 47.2 bits (112), Expect = 2e-08
Identities = 20/73 (27%), Positives = 33/73 (45%)

Query: 326 IAVARQRHRQGLGSQLIDWCEQCARQRGLPALLLEVRPSNRGALAFYERRGFQRIGVRRG 385
IAVA+ ++G+G+ L+ + A++ L+LE + N A FY + F V
Sbjct: 95 IAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAVDTM 154

Query: 386 YYPAGQGQREDAL 398
Y E A+
Sbjct: 155 LYSNFPTANEIAI 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1702IGASERPTASE453e-07 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 45.1 bits (106), Expect = 3e-07
Identities = 30/114 (26%), Positives = 38/114 (33%), Gaps = 15/114 (13%)

Query: 28 KTWLPAAEPATQ--PTRAATAPAAATATTPAAAAPMQEAARDGVAPVREQKPAPAGAPTP 85
+T P AEPA + PT P + T TT P +E + + PV E G
Sbjct: 1137 ETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVV 1196

Query: 86 PRP------AARP-------NVPVPAAREQAQPAAQPVEPARDFDALREQVIQC 126
P +P N P R + VEPA R V C
Sbjct: 1197 ENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALC 1250



Score = 32.3 bits (73), Expect = 0.002
Identities = 17/104 (16%), Positives = 31/104 (29%), Gaps = 6/104 (5%)

Query: 25 GIEKTWLPAAEPATQPTRAATAPAAATATTPAAAAPMQEAARDGVAPVREQKPAPAGAPT 84
G + P E Q + P A V E AP P
Sbjct: 976 GRYDLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEI---ARVDE---APVPPPA 1029

Query: 85 PPRPAARPNVPVPAAREQAQPAAQPVEPARDFDALREQVIQCAA 128
P P+ ++++++ + + A + A +V + A
Sbjct: 1030 PATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAK 1073


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1704GPOSANCHOR522e-08 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 51.6 bits (123), Expect = 2e-08
Identities = 54/328 (16%), Positives = 118/328 (35%), Gaps = 22/328 (6%)

Query: 242 RRETENRLSDTRENLTRVEDILRELGSQLEKLEAQAEVARQYRELQADGEKKQFALWLLK 301
+E E R +D + L + ++++ LEA+ K L
Sbjct: 115 IQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAA--------RKADLEKALEG 166

Query: 302 ETGARDERQRKSQEMAQAQTNLEAAIANLRSGEAELESRRQAHYAAGDAVHAAQGQLYEA 361
K + + + LEA A L E LE A + + +
Sbjct: 167 AMNFSTADSAKIKTLEAEKAALEARQAEL---EKALEGAMNFSTADSAKIKTLEAEKAAL 223

Query: 362 NAQVSRLEAEIRHVVDSRNRLQARREQLQQQIAEWDAQQTHCVEQIAQAEDDLATGAART 421
A+ + LE + ++ A+ + L+ + A +A+Q + + A + +A+
Sbjct: 224 AARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKI 283

Query: 422 EEARALAEEAHASLPAVEARVRDAAASRDEMRSSLARVEQNLALAAQTQRDADRQLQNLE 481
+ A A +E + + A+R +R L + + + Q + E
Sbjct: 284 KTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISE 343

Query: 482 QRRERLQQELRELHAPDPVRLEQLAGDRAAGEDQLEEAQQELAALEARVPEADAERSRAQ 541
R+ L+++ L+ + E + ++ +++ EA + ++
Sbjct: 344 ASRQSLRRD-----------LDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASR 392

Query: 542 AAAQQDAQNLARLEARLAALVKLQEDVQ 569
A +Q + L ++LAAL KL ++++
Sbjct: 393 EAKKQVEKALEEANSKLAALEKLNKELE 420



Score = 47.8 bits (113), Expect = 2e-07
Identities = 56/311 (18%), Positives = 108/311 (34%), Gaps = 37/311 (11%)

Query: 718 DSEQAGLLARQQEIENLQREIKAQQLIADQARAAVARAESAWQQVSQAVAPARQRV---- 773
+ G + + ++A++ +A + +A S A + + +
Sbjct: 126 EKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEK 185

Query: 774 AEITRRVHDIQLEHSRLQQQAEQSGERAARLRQDLEEISAHEEDLRATREEAEARFEALD 833
A + R +++ + + L + ++A + DL E A A
Sbjct: 186 AALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADS 245

Query: 834 AELAEHQSRFADAEIAGEDLAAQAEAARARL---------RELERAAQEAEFAERGVQSR 884
A++ ++ A E +L E A E E+AA EAE A+ QS+
Sbjct: 246 AKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQ 305

Query: 885 IADLQRNRQLAADQSQRAAVELEQLQADLADLD-----ASASQAGLQDALEVRAEREEAL 939
+ + R + R A +QL+A+ L+ + AS+ L+ L+ EA
Sbjct: 306 VLNANRQSLRRDLDASREA--KKQLEAEHQKLEEQNKISEASRQSLRRDLD---ASREAK 360

Query: 940 SRARQELDNLSALLRGADEERMQQERALEPLRARITELQLQEQAARLAEEQFTEQLNARE 999
+ E L + ++ R R L+ A+R A++Q + L
Sbjct: 361 KQLEAEHQKLEEQNKISEASRQSLRRDLD--------------ASREAKKQVEKALEEAN 406

Query: 1000 VDREALAQELA 1010
AL +
Sbjct: 407 SKLAALEKLNK 417



Score = 47.8 bits (113), Expect = 3e-07
Identities = 53/359 (14%), Positives = 118/359 (32%), Gaps = 13/359 (3%)

Query: 214 INRLIEARPEELRVFLEEAAGVSRYKERRRETENRLSDTRENLTRVEDILRELGSQLEKL 273
++ E + + E+A+ + + R+ + E L T ++ L ++ L
Sbjct: 94 LSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 153

Query: 274 EAQAEVARQYRE------LQADGEKKQFALWLLKETGARDERQRKSQEMAQAQTNLEAAI 327
A+ + E + K + E ++ + T A I
Sbjct: 154 AARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKI 213

Query: 328 ANLRSG----EAELESRRQAHYAAGDAVHAAQGQLYEANAQVSRLEAEIRHVVDSRNRLQ 383
L + A +A A + A ++ A+ + LEA + +
Sbjct: 214 KTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAM 273

Query: 384 ARREQLQQQIAEWDAQQTHCVEQIAQAEDDLATGAARTEEARALAEEAHASLPAVEARVR 443
+I +A++ + A E A + R + + + +EA +
Sbjct: 274 NFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQ 333

Query: 444 DAAASRDEMRSSLARVEQNLALAAQTQRDADRQLQNLE---QRRERLQQELRELHAPDPV 500
+S + ++L + + ++ + + Q LE + E +Q LR
Sbjct: 334 KLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASRE 393

Query: 501 RLEQLAGDRAAGEDQLEEAQQELAALEARVPEADAERSRAQAAAQQDAQNLARLEARLA 559
+Q+ +L ++ LE + E++ QA + +A+ L A+ A
Sbjct: 394 AKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQA 452


84Bpet1779Bpet1786N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet1779-1142.091948propionyl-CoA synthetase
Bpet1780-1143.471108hypothetical protein
Bpet17810144.001560putative integral membrane protein
Bpet1782-2143.088996acetyl-CoA synthetase
Bpet17830123.590474putative integral membrane protein
Bpet1784-1123.079903low-specificity L-threonine aldolase
Bpet1785-2132.471425putative cytochrome p450 oxidoreductase
Bpet1786-2161.210805hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1779SACTRNSFRASE300.016 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 30.3 bits (68), Expect = 0.016
Identities = 14/42 (33%), Positives = 18/42 (42%)

Query: 281 TSDIGWVVGHSYIVYGPLIGGQTTILYEGTPVRPDGAILWRL 322
T DI H Y + +IG T+LY P + AI W
Sbjct: 130 TQDINISACHFYAKHHFIIGAVDTMLYSNFPTANEIAIFWYY 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1781TCRTETB340.001 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 33.7 bits (77), Expect = 0.001
Identities = 26/104 (25%), Positives = 41/104 (39%), Gaps = 7/104 (6%)

Query: 287 AAGAFAFGYLQDRIGHKRALGITLAGWIVMVLVAYAAVTAPVFWAAAILAGLCMGTSQSA 346
+ G +G L D++G KR L L G I+ + F++ I+A G +A
Sbjct: 63 SIGTAVYGKLSDQLGIKRLL---LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAA 119

Query: 347 GRAM----VGALAPPARLAEFFALWTFAVQLAAVVGPLTYGLVT 386
A+ V P + F L V + VGP G++
Sbjct: 120 FPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA 163


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1784PRTACTNFAMLY300.015 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 30.0 bits (67), Expect = 0.015
Identities = 29/115 (25%), Positives = 39/115 (33%), Gaps = 3/115 (2%)

Query: 91 GAAVLGSIQPQPIEHAADGTLPLDKLAAAVKPQGDPHFARTRLLALENTFQGKVIPAGYI 150
G +G++Q E + L P A + L A E T G I G
Sbjct: 181 GGLHIGALQSLQPEDLPPSRVVLRDTNVTAVPASGAPAAVSVLGASELTLDGGHITGG-- 238

Query: 151 DQAAAFARQHGLGLHLDGARVFNAAVASGRPVQDVCAPFDSVSICFSKGLGAPVG 205
+AA A G +HL A + +G V P +V F G PV
Sbjct: 239 -RAAGVAAMQGAVVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPGGFGPVL 292


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1786NUCEPIMERASE414e-06 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 40.5 bits (95), Expect = 4e-06
Identities = 42/188 (22%), Positives = 69/188 (36%), Gaps = 30/188 (15%)

Query: 4 RVLLAG-CGDLGLRLARRLLADGAEVWAL------------RRQPPANEPGGIHWLRADL 50
+ L+ G G +G +++RLL G +V + + + G + + DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 51 TQPDTLAGLPA--GITQLAYLPA-PGAR----DPAVYQAVFREGLPALLAALDTRALQRV 103
+ + L A ++ P R +P Y G +L +Q +
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 104 LFVSSSAVYG-NHDGGWVDETTPPAPAGFNGRVLLDTENWLAAQA------LPSVSLRLA 156
L+ SSS+VYG N + + + P E L A LP+ LR
Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANE--LMAHTYSHLYGLPATGLRFF 179

Query: 157 GLYGP-GR 163
+YGP GR
Sbjct: 180 TVYGPWGR 187


85Bpet1995Bpet2003N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet19951114.391938putative outer membrane lipoprotein
Bpet19962135.017292hypothetical protein
Bpet1997-3112.956805putative secreted protein
Bpet1998-3112.629386putative general secretion pathway ATPase
Bpet1999-2142.212396putative general secretion pathway protein
Bpet20000151.546675putative prepilin protein
Bpet2001-1151.810107putative prepilin protein
Bpet2002-1180.991961alanyl-tRNA synthetase
Bpet2003-2160.653359hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1995BCTERIALGSPD486e-08 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 48.0 bits (114), Expect = 6e-08
Identities = 58/296 (19%), Positives = 105/296 (35%), Gaps = 46/296 (15%)

Query: 256 SLVVTDIPDVLDRIGQFIERENQALTRRVRLLFEEI--TVVANDSAEGGIDWKAVYDSAR 313
+L+VT PDV++ + + I Q RR ++L E I V D GI W
Sbjct: 320 ALIVTAAPDVMNDLERVI---AQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKNAGMT 376

Query: 314 AAVAATLPVA-----------AGGAAAALGATVDS------GPFQGT-RAIVSALSQTGA 355
+ LP++ G +++L + + S G +QG +++ALS +
Sbjct: 377 QFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGNWAMLLTALSSSTK 436

Query: 356 VLRHSSVPVLTLNRRPVTHAVRTTFSYIDQVQSTAVPGIDAALGSTALPSVSISQKQETV 415
++ ++TL+ T V + Q+T S ++ +K TV
Sbjct: 437 NDILATPSIVTLDNMEATFNVGQEVPVLTGSQTT----------SGDNIFNTVERK--TV 484

Query: 416 GTFLTLVPDAQADGRILLSIAYDNTVAQPIKSVTFGTQGNQIQVQQITIDGNGTVQQVAL 475
G L + P +LL + Q + SV T + V +
Sbjct: 485 GIKLKVKPQINEGDSVLL------EIEQEVSSVADAASSTS-SDLGATFNTRTVNNAVLV 537

Query: 476 SPGQPVILSGF--DRRQDEYDRRRLSADAPLLAG--GQDRASSERLTTVVLVTAQV 527
G+ V++ G D D+ L D P++ + ++ + V
Sbjct: 538 GSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTV 593



Score = 30.3 bits (68), Expect = 0.021
Identities = 16/63 (25%), Positives = 24/63 (38%), Gaps = 6/63 (9%)

Query: 229 ALQAVRVRIL-----PFLTQAGTIADLDGGGS-SLVVTDIPDVLDRIGQFIERENQALTR 282
L V R L AG + + S L++T V+ R+ +ER + A R
Sbjct: 133 PLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR 192

Query: 283 RVR 285
V
Sbjct: 193 SVV 195


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet1999BCTERIALGSPF447e-07 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 43.7 bits (103), Expect = 7e-07
Identities = 39/161 (24%), Positives = 62/161 (38%), Gaps = 22/161 (13%)

Query: 96 CQLVAAAQQAGGEALPHALRDLAGAARLVQQARGTLAGT----CAAGGAALAVAVGLLCA 151
C +VAA + +G L L LA QQ R + C A+AV LL
Sbjct: 136 CAMVAAGETSG--HLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLTVVAIAVVSILLSV 193

Query: 152 VPYFTVPRLQHVF----QAVPADYYGGLTQGLYALAQALRRWLAFWAVLLAGGAWLAAWS 207
V VP++ F QA+P T+ L ++ A+R + + + L G
Sbjct: 194 V----VPKVVEQFIHMKQALPL-----STRVLMGMSDAVRTFGPWMLLALLAGFMAFRVM 244

Query: 208 L--PAFTGPWRARLDRL-VPWRLYRDFHAIRFLAMLAVMLR 245
L + RL L + R+ R + R+ L+++
Sbjct: 245 LRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNA 285


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2000PilS_PF08805417e-07 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 41.1 bits (96), Expect = 7e-07
Identities = 32/192 (16%), Positives = 65/192 (33%), Gaps = 32/192 (16%)

Query: 11 RRQAGFSLIEVSIVTAIVLLVAIIGIPAIGAYVIENKVPKVGEELQRFVARTKTFAQGSG 70
+ G +L+EV +V +++++A + + +A K+ + G
Sbjct: 23 EQDKGATLMEVLLVVGVIVVLAASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSL-KFQG 81

Query: 71 PAPYADIDTGALANALRDSSVVAVAGTGAAAVVSHGLGGSGSGSNGTITVAPAAVAGGGA 130
++ L S + TGA+A G G++T+ +
Sbjct: 82 RYTDSNY-IKTLYAQGLLPSDMIADTTGASAKNPWG---------GSVTITTS-----SD 126

Query: 131 GSGFVITLTNVSNAACPGLASVMQRVSDIITVEGRGGAAKVKDITIVPRLAYSAAAAESQ 190
F + NV C + + ++ I+ + + S +A +
Sbjct: 127 KYSFNVVEANVPQKNCMAMVNALRS---------------SSAISKINNTSTSTVSAATV 171

Query: 191 CAEGDSNTFVFT 202
CA DSNT F+
Sbjct: 172 CA-SDSNTLTFS 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2001BCTERIALGSPG310.004 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 30.6 bits (69), Expect = 0.004
Identities = 15/57 (26%), Positives = 27/57 (47%)

Query: 15 RPGRRQAGFALLELTLAVALAGMLLVWGANRLVHRIDDAAGQATGAWMLELKRGLDN 71
R +Q GF LLE+ + + + G+L L+ + A Q + ++ L+ LD
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDM 58


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2003PF01206901e-27 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 89.8 bits (223), Expect = 1e-27
Identities = 37/77 (48%), Positives = 53/77 (68%), Gaps = 2/77 (2%)

Query: 10 LPEFQHEVDASGLTCPLPILRAKKALAQMESGQVLRVATTDPKATRDFQAFAKQSGNALL 69
+ EF +DA+GL CPLPIL+AKK LA M +G+VL V TDP + +DF++F+KQ+G+ LL
Sbjct: 1 MAEFDQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELL 60

Query: 70 AQHDNNQGTVLHFLRRR 86
Q + + HF +R
Sbjct: 61 EQKE--EDGTYHFRLKR 75


86Bpet2077Bpet2086N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet20772122.365833putative outer membrane protein
Bpet20783122.494477putative general secretion pathway ATPase
Bpet2079292.344233hypothetical protein
Bpet20800130.976197hypothetical protein
Bpet2081-212-1.089552hypothetical protein
Bpet2082-314-2.799648putative methyltransferase
Bpet2083-216-3.511133hypothetical protein
Bpet2084-216-3.565042inosine-5'-monophosphate dehydrogenase
Bpet2085-223-4.048059GMP synthase
Bpet2086-337-4.533894hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2077BCTERIALGSPD1501e-41 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 150 bits (380), Expect = 1e-41
Identities = 68/253 (26%), Positives = 113/253 (44%), Gaps = 20/253 (7%)

Query: 161 NMVLLDVQVVEIPSARLREFGLQWDALSQGGLHAGGV-WQPGSSLQLAD---------AA 210
VL++ + E+ A G+QW + G +++ A+ ++
Sbjct: 345 PQVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSS 404

Query: 211 QPPALSMQGMGAAGYFGVNALLSARLAALAQRGEAVMLAQPQLLARSGTTASFLAGGEVP 270
ALS AAG++ N + L AL+ + +LA P ++ A+F G EVP
Sbjct: 405 LASALSSFNGIAAGFYQGN--WAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVP 462

Query: 271 Y---STTDAQGNS--STEFKPYGVSLNITPRIDRNGAIRSRIEVEASSIDTSLSVAG--- 322
S T + N + E K G+ L + P+I+ ++ IE E SS+ + S
Sbjct: 463 VLTGSQTTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDL 522

Query: 323 GPALRTRRAVTEFNVRSGQTLVLGGFLSRERSHERSGLPVLQDIPLLGALFSSRRDQHKE 382
G TR V SG+T+V+GG L + S +P+L DIP++GALF S + +
Sbjct: 523 GATFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSK 582

Query: 383 TELAIFVTPRIVS 395
L +F+ P ++
Sbjct: 583 RNLMLFIRPTVIR 595


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2079BCTERIALGSPF320.002 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 32.1 bits (73), Expect = 0.002
Identities = 33/142 (23%), Positives = 58/142 (40%), Gaps = 12/142 (8%)

Query: 97 RLRGRRLARFEQQLPGALLALASALRAGVGVSTALRHIVDHSEPPLAQEFGLMLREQRLG 156
RL LA +QL A+ + A + + AL + SE P ++ R
Sbjct: 64 RLSTSDLALLTRQL-------ATLVAASMPLEEALDAVAKQSEKP---HLSQLMAAVRSK 113

Query: 157 VSFDAALARLSQRVPSEASALVAAALRVATHTGGNLAETLDGIARTLRERLQLQGKVR-A 215
V +LA + P L A + A T G+L L+ +A +R Q++ +++ A
Sbjct: 114 VMEGHSLADAMKCFPGSFERLYCAMVA-AGETSGHLDAVLNRLADYTEQRQQMRSRIQQA 172

Query: 216 LTAQGRLQAWIVGALPLLLAAV 237
+ L + + +LL+ V
Sbjct: 173 MIYPCVLTVVAIAVVSILLSVV 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2080BCTERIALGSPF320.002 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 32.1 bits (73), Expect = 0.002
Identities = 13/50 (26%), Positives = 23/50 (46%)

Query: 192 MRAGMPRAAALKALADRADSPAVRSWIAALTQADSLGMSLGAVLRGHAAQ 241
+ A MP AL A+A +++ P + +AA+ G SL ++
Sbjct: 81 VAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLADAMKCFPGS 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2084HTHFIS310.013 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.013
Identities = 13/70 (18%), Positives = 25/70 (35%), Gaps = 5/70 (7%)

Query: 217 RVGAAVGVGAGTEERVEKLAAAGVDVIIVDTAHGHSAGVLERVRWVKQNYPKVEVI---- 272
R G V + + +AA D+++ D + + +K+ P + V+
Sbjct: 25 RAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA-FDLLPRIKKARPDLPVLVMSA 83

Query: 273 GGNIATAAAA 282
TA A
Sbjct: 84 QNTFMTAIKA 93


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet208660KDINNERMP280.009 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 28.4 bits (63), Expect = 0.009
Identities = 12/76 (15%), Positives = 28/76 (36%), Gaps = 6/76 (7%)

Query: 7 PYLVAAHVTAVVFLVGGLLAQERMVNAISQSPPQEQIGMLAALLRFDRLVTTPA-LLLTW 65
PY + + + + ++M P Q++I ++ + P+ L+L +
Sbjct: 463 PYYILP-----ILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYY 517

Query: 66 IFGLSLALSAGWLSSR 81
I + + L R
Sbjct: 518 IVSNLVTIIQQQLIYR 533


87Bpet2094Bpet2113N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2094012-2.482491transposase
Bpet2095-113-1.879089flagellin
Bpet2096117-0.684283flagellar-specific RNA polymerase sigma factor
Bpet2097-120-0.633282transcriptional activator FlhD
Bpet2098-118-0.646879transcriptional activator FlhC
Bpet2099-120-0.587948flagellar motor protein MotA
Bpet2100-119-0.452691flagellar motor protein MotB
Bpet2101-1200.005072chemotaxis protein CheY
Bpet2102021-0.536015chemotaxis protein CheA
Bpet2103021-0.632801chemotaxis protein CheW
Bpet2104-1220.972098hypothetical protein
Bpet2105-1191.585354chemotaxis protein methyltransferase
Bpet21060181.446721chemotaxis-specific methylesterase
Bpet2107-2150.317599chemotaxis protein CheY
Bpet2108-2160.739426chemotaxis regulator CheZ
Bpet2109-1151.247626hypothetical protein
Bpet21101162.671130flagellar biosynthesis protein FlhB
Bpet21111152.561662hypothetical protein
Bpet21122143.163129transposase
Bpet21133163.935530transposase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2094HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2095FLAGELLIN2496e-79 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 249 bits (637), Expect = 6e-79
Identities = 236/507 (46%), Positives = 286/507 (56%), Gaps = 7/507 (1%)

Query: 2 AAVINTNYLSLVAQNNLNKSQSALGSAIERLSSGLRINSAKDDAAGQAIANRFTANVRGL 61
A VINTN LSL+ QNNLNKSQS+L SAIERLSSGLRINSAKDDAAGQAIANRFT+N++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 TQAARNANDGISIAQTTEGALNEINNNLQRIRELTVQASSGTNSPSDLESIQNEITQRLG 121
TQA+RNANDGISIAQTTEGALNEINNNLQR+REL+VQA++GTNS SDL+SIQ+EI QRL
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EITRVSEQTQFNGVKVLANDQSLTIQVGANDNETITIDLKQVNATTLGLDKLDVGSQLTS 181
EI RVS QTQFNGVKVL+ D + IQVGAND ETITIDL++++ +LGLD +V +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEA 180

Query: 182 KNFANVTTIAASAPAEWTDFSFAVEGGSTFTLAVGTDNQLYATDGTDYYEATFNADTGTV 241
++ + + AV TD Y A T
Sbjct: 181 TVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDD 240

Query: 242 TVGAITAGIAASNINADATEFDTADGVVTLADAEAATLVEPAAPSVSTVYMDNTGTTTAY 301
+ + + T A E T N G
Sbjct: 241 AENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVS 300

Query: 302 YVKAGGTYYDAEIDLDTGEVSVNLGSATSTLAAGTAVTAQAVLTAAADPGVAVDLSTVNS 361
G D+ G +V+ + S+ T+V + LS + +
Sbjct: 301 TTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEA 360

Query: 362 TFSGTNSLVRDSETGAYYVKNVSGDKTSYYEATVDLDTGVVTATADDEIVV-------DP 414
+ Y T + T +T +E +P
Sbjct: 361 NNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANP 420

Query: 415 LNAIDNALSAVDSLRSDLGAIQNRFESTITNLNNTVNNLSAARSRIEDADYATEVSNMTK 474
L +ID+ALS VD++RS LGAIQNRF+S ITNL NTV NL++ARSRIEDADYATEVSNM+K
Sbjct: 421 LASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSK 480

Query: 475 AQILQQAGTSVLAQANQVPQTVLSLLR 501
AQILQQAGTSVLAQANQVPQ VLSLLR
Sbjct: 481 AQILQQAGTSVLAQANQVPQNVLSLLR 507


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2099ACRIFLAVINRP377e-05 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 37.1 bits (86), Expect = 7e-05
Identities = 17/44 (38%), Positives = 27/44 (61%)

Query: 4 VIGYAVVLVAVIGSFAALGGHMGALYQPFELTLIAGGALGAFLA 47
++G A+VL AV A GG GA+Y+ F +T+++ AL +A
Sbjct: 442 LVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVA 485


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2100OMPADOMAIN498e-09 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 49.2 bits (117), Expect = 8e-09
Identities = 29/120 (24%), Positives = 53/120 (44%), Gaps = 13/120 (10%)

Query: 167 FATGSAEVQPYMRDILRELGPVLNEL---PNKISIAGHTDATQYARGERAYSNWELSADR 223
F A ++P + L +L L+ L + + G+TD G AY N LS R
Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDR----IGSDAY-NQGLSERR 277

Query: 224 ANASRQELVAGGMAEGKLMRIQGLSSSMSLVKDDPYAAVNRR---ISLVVLNRRTQQQIE 280
A + L++ G+ K + +G+ S + V + V +R I + +RR + +++
Sbjct: 278 AQSVVDYLISKGIPADK-ISARGMGES-NPVTGNTCDNVKQRAALIDCLAPDRRVEIEVK 335


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2101HTHFIS808e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 79.9 bits (197), Expect = 8e-21
Identities = 36/121 (29%), Positives = 55/121 (45%), Gaps = 2/121 (1%)

Query: 3 ATILVADDSATMRMIVQATLTEAGWRVLTAGNGQQALELARGNRVDMLVSDWNMPVMGGL 62
ATILVADD A +R ++ L+ AG+ V N D++V+D MP
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 63 ALIQGLRGEPGYQDLPVLVLTTEDDVQSKDAARGLGVCGWLNKPLDPGVLVELASELLGE 122
L+ ++ DLPVLV++ ++ + A G +L KP D L+ + L E
Sbjct: 64 DLLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 123 Q 123

Sbjct: 122 P 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2102PF06580411e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 41.4 bits (97), Expect = 1e-05
Identities = 13/78 (16%), Positives = 33/78 (42%), Gaps = 10/78 (12%)

Query: 408 ELDKSLIERIIDPLT--HLVRNSLDHGIETPEKRIAAGKDPVGQLILSAEHNGGNIVIEV 465
+++ ++++ + P+ LV N + HGI G+++L + G + +EV
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIA--------QLPQGGKILLKGTKDNGTVTLEV 296

Query: 466 SDDGAGLNRDKILKKAIA 483
+ G+ ++
Sbjct: 297 ENTGSLALKNTKESTGTG 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2106HTHFIS681e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.5 bits (165), Expect = 1e-14
Identities = 44/191 (23%), Positives = 81/191 (42%), Gaps = 26/191 (13%)

Query: 2 MKKIRVLCVDDSALVRGLMTEIINSHDDMEVVAVAPDPLVARELIKQHNPDVLTLDVEMP 61
M +L DD A +R ++ + ++ +V + + I + D++ DV MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSR-AGYDVRITS-NAATLWRWIAAGDGDLVVTDVVMP 58

Query: 62 RMDGLDFLEKLMRLRP-MPVVMVSSLTERGGETTLRALELGAIDFVTKPKLGIRHGMLEY 120
+ D L ++ + RP +PV+++S+ T ++A E GA D++ KP +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMSAQNT--FMTAIKASEKGAYDYLPKP--------FDL 108

Query: 121 SELIADKIRAAARARLRVPAASAQAAPPARLRSPFASSEKLVIVGASTGGTEAIREVLRP 180
+ELI RA A + R + + + +VG S E R + R
Sbjct: 109 TELIGIIGRALAEPKRRPSKLEDDS------------QDGMPLVGRSAAMQEIYRVLARL 156

Query: 181 LPPDSPAVLIT 191
+ D ++IT
Sbjct: 157 MQTDLT-LMIT 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2107HTHFIS851e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 84.9 bits (210), Expect = 1e-22
Identities = 31/105 (29%), Positives = 53/105 (50%), Gaps = 3/105 (2%)

Query: 7 KILVVDDFPTMRRIIRNLLKELGFENVDEAEDGAIGLEKLRNGSFQFVVSDWNMPNLDGL 66
ILV DD +R ++ L G++ V + A + G VV+D MP+ +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYD-VRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 67 EMLKQIRADGALKSLPVLMVTAEAKKENIVAAAQAGANGYVVKPF 111
++L +I+ A LPVL+++A+ + A++ GA Y+ KPF
Sbjct: 64 DLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2109PF05272300.020 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.020
Identities = 28/126 (22%), Positives = 40/126 (31%), Gaps = 18/126 (14%)

Query: 49 ARPLHRFLARMRGHVVAVRQASIQIALNAAR---TQYQASQCASLAREQAAAAEALAGSG 105
A LH +LA R + R T Q A L RE A AAE A G
Sbjct: 731 AEALHLYLAGERYFPSPEDEEIYFRPEQELRLVETGVQGRLWALLTREGAPAAEGAAQKG 790

Query: 106 SQIEQLSDAASAHMREIAQ-VSARNLEAARAALLALGDVMGRME-------RMTAEMAGL 157
+ + IA V A + +++ + G V + R T+
Sbjct: 791 Y-------SVNTTFVTIADLVQALGADPGKSSPMLEGQVRDWLNENGWEYLRETSGQRRR 843

Query: 158 AGVVEQ 163
+ Q
Sbjct: 844 GYMRPQ 849


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2110TYPE3IMSPROT357e-125 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 357 bits (919), Expect = e-125
Identities = 98/343 (28%), Positives = 174/343 (50%), Gaps = 3/343 (0%)

Query: 8 EKTEAASPRRLEKAREEGQIARSRELGTFLLLAAGVGGLWLSGSMLYRGLTGVLRNGLGF 67
EKTE +P+++ AR++GQ+A+S+E+ + L+ A L + + ++ +
Sbjct: 4 EKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLML--IPA 61

Query: 68 DARIGRDPGIMVAQAVDGAGQALLLVLPIFGVLMAVAVLASVVLGGFVFSAKPLQPDFAK 127
+ + + + L P+ V +A+ + VV GF+ S + ++PD K
Sbjct: 62 EQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKK 121

Query: 128 LSLFSGLKRMVSAQTVVELLKAVAKALLVGGVAVAVIGGHRDEMLALMHAAPTEALVKAL 187
++ G KR+ S +++VE LK++ K +L+ + +I G+ +L L
Sbjct: 122 INPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLG 181

Query: 188 TLVALCAALIVASLGIIVLLDVPWQIWSHLKKLRMSKEDVRQEHKESEGDPHIKARIRQQ 247
++ + +I + D ++ + ++K+L+MSK+++++E+KE EG P IK++ RQ
Sbjct: 182 QILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQF 241

Query: 248 QRSMARRRMMSEVPRADVVVTNPTHYAVALRYAEGQA-APRVVAKGTGLVAARIRELAAG 306
+ + R M V R+ VVV NPTH A+ + Y G+ P V K T +R++A
Sbjct: 242 HQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEE 301

Query: 307 HRVPLLQAPPLARALYQHVELGQEIPAALYTAVAEVLAWVFQL 349
VP+LQ PLARALY + IPA A AEVL W+ +
Sbjct: 302 EGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQ 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2113HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


88Bpet2124Bpet2151N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2124-1150.484870hypothetical protein
Bpet2125-1160.582589flagellar basal body rod protein FlgF
Bpet2126-115-0.064865flagellar basal body rod protein FlgG
Bpet2127-2170.736178flagellar basal body L-ring protein
Bpet2128-2180.888248flagellar basal body P-ring protein
Bpet2129-1210.342589flagellum-specific peptidoglycan hydrolase
Bpet2130-121-0.140501flagellar hook-associated protein FlgK
Bpet2131-122-0.146313flagellar hook-associated protein 3
Bpet2132-1220.028916hypothetical protein
Bpet2133120-0.312564putative lipoprotein
Bpet2134118-0.126219hypothetical protein
Bpet2135215-0.524232flagellar biosynthesis protein FliR
Bpet2136115-0.170473flagellar biosynthesis protein FliQ
Bpet2137-2123.503170flagellar biosynthesis protein FliP
Bpet2138-2124.279429flagellar biosynthesis protein FliO
Bpet2139-2114.140149flagellar motor switch protein FliN
Bpet2140-1124.415109flagellar motor switch protein FliM
Bpet21410124.628271flagellar basal body-associated protein FliL
Bpet2142-1124.455388flagellar hook-length control protein FliK
Bpet2143-1152.355444flagellar biosynthesis chaperone FliJ
Bpet2144-1142.455605flagellar biosynthesis ATPase FliI
Bpet2145-1141.614968flagellar assembly protein H
Bpet2146-2102.869942flagellar motor switch protein G
Bpet2147-293.955060flagellar MS-ring protein
Bpet2148-1114.276696flagellar hook-basal body complex protein FliE
Bpet2149-2142.944200hypothetical protein
Bpet2150-2142.886354hypothetical protein
Bpet2151-1153.120915hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2124FLGHOOKAP1415e-06 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 41.5 bits (97), Expect = 5e-06
Identities = 26/119 (21%), Positives = 51/119 (42%), Gaps = 9/119 (7%)

Query: 296 SGEYSGLSINSDGTLQANYTNGETAIIGTLALAN-FNNVQGLQPVGNNAWAETGASGQPT 354
S E +G S N +G + + + G + + + ++ +GN + T
Sbjct: 436 SEEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSD--IGNKT------ATLKT 487

Query: 355 LGQPGTNGLATVVGQAVEASNVDMSKELVNMIVAQRTYQANAQTIKTQDEVMQVLMNMR 413
N + + Q S V++ +E N+ Q+ Y ANAQ ++T + + L+N+R
Sbjct: 488 SSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 38.0 bits (88), Expect = 6e-05
Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 4/56 (7%)

Query: 6 GLSGLNAAAQNLDVIGNNIANSGTVGFKSATASFAD----VYASSRVGLGVKVSAI 57
+SGLNAA L+ NNI++ G+ T A + A VG GV VS +
Sbjct: 7 AMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2126FLGHOOKAP1436e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 43.0 bits (101), Expect = 6e-07
Identities = 17/80 (21%), Positives = 31/80 (38%), Gaps = 14/80 (17%)

Query: 4 SLWIAKTGLEGQQTSMDVISNNLANVQTNGFKRGRAIFQDLMYQTLRQPGAQVGDANQLP 63
+ A +GL Q +++ SNN+++ G+ R I + L
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI--------------MAQANSTLG 48

Query: 64 TGLQLGTGVRVASTERVFTQ 83
G +G GV V+ +R +
Sbjct: 49 AGGWVGNGVYVSGVQREYDA 68



Score = 43.0 bits (101), Expect = 7e-07
Identities = 12/48 (25%), Positives = 24/48 (50%)

Query: 214 SILQQYVETSNVNVAEELVNMITTQRAYEMNSKAVKTSDEMLARLTQL 261
+ Q S VN+ EE N+ Q+ Y N++ ++T++ + L +
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2127FLGLRINGFLGH2063e-69 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 206 bits (525), Expect = 3e-69
Identities = 120/222 (54%), Positives = 155/222 (69%), Gaps = 6/222 (2%)

Query: 9 AAVLALAAAGCAMIPPEPVVTGPVTAAPPPPPMPAAQPNGSIY---QPRVYGNYPLFEDR 65
+++L L+ GCA IP P+V G +A P P P P A NGSI+ QP YG PLFEDR
Sbjct: 12 SSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVA--NGSIFQSAQPINYGYQPLFEDR 69

Query: 66 RPRNVGDIVTIVLNERTNAAKNVATNTDRSGNVGLGIAATPGFMDSW-ANAKLNTDASGS 124
RPRN+GD +TIVL E +A+K+ + N R G G P ++ NA+ + +ASG
Sbjct: 70 RPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGG 129

Query: 125 NKASGKGDSSANNTFTGTITTTVIGVLPNGNLQVAGEKQIAINRGSEYVRFSGVVDPRSI 184
N +GKG ++A+NTF+GT+T TV VL NGNL V GEKQIAIN+G+E++RFSGVV+PR+I
Sbjct: 130 NTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTI 189

Query: 185 TGANSVSSTQVADARIEYRSKGVMDEVQTMGWLQRFFLIASP 226
+G+N+V STQVADARIEY G ++E Q MGWLQRFFL SP
Sbjct: 190 SGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSP 231


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2128FLGPRINGFLGI380e-133 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 380 bits (977), Expect = e-133
Identities = 160/368 (43%), Positives = 219/368 (59%), Gaps = 11/368 (2%)

Query: 13 LAGCVLAAALLMLAGPAQAE--RIKDLASIQGVRGNPLIGYGLVVGLDGSGDQVRQTPFT 70
A V +A + PAQA+ RIKD+AS+Q R N LIGYGLVVGL G+GD +R +PFT
Sbjct: 8 AAALVFSALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFT 67

Query: 71 QQSLTNMLSQLGITVPPGSNMQLKNVAAVMVTATLPAFARPGQRLDVVVSSMGNAKSLRG 130
+QS+ ML LGIT G + KN+AAVMVTA LP FA PG R+DV VSS+G+A SLRG
Sbjct: 68 EQSMRAMLQNLGITTQGGQS-NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRG 126

Query: 131 GTLLMTPLKGADSQVYAIAQGNILVGGAGASAGGSSVQINQLNGGRINDGAIVERGVPTS 190
G L+MT L GAD Q+YA+AQG ++V G A +++ R+ +GAI+ER +P+
Sbjct: 127 GNLIMTSLSGADGQIYAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSK 186

Query: 191 FSRDGLIYLEMDVTDFGTTQNVVAALNR----QFGAGTAEAVDGRVVQVRGPLDAAEQAA 246
F + L++ DF T V +N ++G AE D + + V+ P A+
Sbjct: 187 FKDSVNLVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKP-RVADLTR 245

Query: 247 FLARVENLQVRRAPATAKVIINARTGSVVMNQTVMIDEAAVAHGNLSVIINRQTQVSQPD 306
+A +ENL V AKV+IN RTG++V+ V I AV++G L+V + QV QP
Sbjct: 246 LMAEIENLTV-ETDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQP- 303

Query: 307 TPFGGGQTVVVPNTQIEVRQEDGALHRVRTSANLADVVKALNALGATPQDLLAILQAMKS 366
PF GQT V P T I QE + + +L +V LN++G ++AILQ +KS
Sbjct: 304 APFSRGQTAVQPQTDIMAMQEGSKV-AIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKS 362

Query: 367 AGALRADL 374
AGAL+A+L
Sbjct: 363 AGALQAEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2129FLGFLGJ2095e-68 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 209 bits (532), Expect = 5e-68
Identities = 115/279 (41%), Positives = 168/279 (60%), Gaps = 9/279 (3%)

Query: 29 VRAKPGDSAAQ--KQVATQVEALFLQMMLKRMREAGPKSGLFDSQQSQMMQSMADEQLAL 86
++AK G+ A + VA QVE +F+QMMLK MR+A PK GLF S+ +++ SM D+Q+A
Sbjct: 21 LKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQ 80

Query: 87 QL-ARPGVGLAQAMLRQMQQGRPAGVADAALDGV--LQNDSAPRRVTALLDVLRNNRASD 143
Q+ A G+GLA+ M++QM +P + + AL +++ +
Sbjct: 81 QMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRN 140

Query: 144 RALAAAEGAPVHVVDFVSRMAGPAQEAARQTGVPARLILGQAALESGWGRRELKYDNGAT 203
+ P F+++++ PAQ A++Q+GVP LIL QAALESGWG+R+++ +NG
Sbjct: 141 ----YDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEP 196

Query: 204 SYNLFGIKAGSSWNGKVVNVLTTEYEDGVARKVVQPFRAYGSYEESFADYARLIGENPRY 263
SYNLFG+KA +W G V + TTEYE+G A+KV FR Y SY E+ +DY L+ NPRY
Sbjct: 197 SYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRY 256

Query: 264 EPVLQARDEIDAARRIQAAGYATDPAYADKLIAIMGQLR 302
V A A+ +Q AGYATDP YA KL ++ Q++
Sbjct: 257 AAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMK 295


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2130FLGHOOKAP1355e-118 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 355 bits (911), Expect = e-118
Identities = 221/551 (40%), Positives = 338/551 (61%), Gaps = 9/551 (1%)

Query: 2 NLYNLALTGLNASQAGMEVTSHNINNAANAGYSRQRLVTSTAGATETGQGFFGRGVQVDT 61
+L N A++GLNA+QA + S+NI++ AGY+RQ + + A +T G+ G GV V
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSG 61

Query: 62 VKRQYDSFLYRQMVGAQGTGAQLATHYDQVSQINNLFGDRTVGITPALENFFASLNAGAS 121
V+R+YD+F+ Q+ AQ + L Y+Q+S+I+N+ T + +++FF SL S
Sbjct: 62 VQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVS 121

Query: 122 NPADPAVRQDILGKTNSLVTQINTAYRELQNLREGVNTQISTTVEQVNSYLERINDLNKQ 181
N DPA RQ ++GK+ LV Q T + L++ + VN I +V+Q+N+Y ++I LN Q
Sbjct: 122 NAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLNDQ 181

Query: 182 IVVAQG-KSGHAPNDLLDQRDQALSELNQLVGVRYY-EQGNSLNITLQSGQTLLSGTTVY 239
I G +G +PN+LLDQRDQ +SELNQ+VGV + G + NIT+ +G +L+ G+T
Sbjct: 182 ISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTAR 241

Query: 240 PLAAVQSASDPTRMALAYSLPAGSGSTVQVELSDSEISGGKLAGFLQFRSQSLDAVQDQL 299
LAAV S++DP+R +AY G+ +E+ + ++ G L G L FRSQ LD ++ L
Sbjct: 242 QLAAVPSSADPSRTTVAYV----DGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTL 297

Query: 300 GQLAIGLALAFNAQHEAGLDLNGDPGEALFGLSQPAAIPKVGNTGDGSLTAEFTDASAIH 359
GQLA+ A AFN QH+AG D NGD GE F + +PA + N GD ++ A TDASA+
Sbjct: 298 GQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDASAVL 357

Query: 360 ATSYDIVYNGTQYVVTRQSDGAVQTLTPSADTPPTIEFDGMTVTVDGTPAAGDVWKLQAT 419
AT Y I ++ Q+ VTR + T+TP A+ + FDG+ +T GTPA D + L+
Sbjct: 358 ATDYKISFDNNQWQVTRLASNTTFTVTPDANG--KVAFDGLELTFTGTPAVNDSFTLKPV 415

Query: 420 RDAARDLKALITDPAKLALA-DAAGGTTNGANGLLLAKLQTEKVLGGGTLSLSGQFSQLI 478
DA ++ LITD AK+A+A + G ++ NG L LQ+ GG S + ++ L+
Sbjct: 416 SDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLV 475

Query: 479 NNIGVQTQQIKTAATAQDNLITQQTSAYLSVSGVNLNEEYVNLTIYQEQYQASAKILDVA 538
++IG +T +KT++ Q N++TQ ++ S+SGVNL+EEY NL +Q+ Y A+A++L A
Sbjct: 476 SDIGNKTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTA 535

Query: 539 STVFDTLLGLR 549
+ +FD L+ +R
Sbjct: 536 NAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2131FLAGELLIN462e-07 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 45.8 bits (108), Expect = 2e-07
Identities = 49/239 (20%), Positives = 88/239 (36%), Gaps = 9/239 (3%)

Query: 1 MRLSTALIYQNGLNGILKQESTLSRLQEQLASGRRVLTPADDPLAASLAVNVSQTSSMNA 60
++T + N + K +S+LS E+L+SG R+ + DD AA A+ TS++
Sbjct: 2 QVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD--AAGQAIANRFTSNIKG 59

Query: 61 TYASNRNT--AKQTLGLESNALSSVVTTLQSVLQRVVQAG-GTLSDPDRQALVTELEASR 117
++RN AL+ + LQ V + VQA GT SD D +++ E++
Sbjct: 60 LTQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRL 119

Query: 118 DQLVGLANSTDGNGQYLFSGHQGFSAPVTLDADGNVSYGGDN----GQRLIQVDQSRQMA 173
+++ ++N T NG + S V + ++ L + +
Sbjct: 120 EEIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 174 GSDVISDIFGKASAGSKAYIVEANEANTGTGQFSSVSFDTTTGSGANQDFIVTFSDVGG 232
+ K G Y V AN+ + V+ T +
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTT 238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2132PF03544320.006 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 31.9 bits (72), Expect = 0.006
Identities = 12/128 (9%), Positives = 26/128 (20%), Gaps = 8/128 (6%)

Query: 520 IEVPAQQLTAQHAPRVAESARQSAATATTAARGAEPAPSAPLRQLPGATTQAPAPAAAAA 579
+ + A ++ + EP P P P P
Sbjct: 52 VTMVAPADLEP-----PQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPK 106

Query: 580 APR-TARSVRRADQASAPAESTASSVTRRAAAAQPQAEG--ASASRPARRPTVAATGPIA 636
+ + R + S + + + A P +
Sbjct: 107 PVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQ 166

Query: 637 ANSAARRA 644
+ A+
Sbjct: 167 YPARAQAL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2135TYPE3IMRPROT1681e-53 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 168 bits (428), Expect = 1e-53
Identities = 123/256 (48%), Positives = 183/256 (71%), Gaps = 1/256 (0%)

Query: 1 MINFTQQQLDAWLLQFLWPFVRMLALVGSAPLFSESTIPIRIKVALAFMLTVAVAPGLEP 60
M+ T +Q +WL + WP +R+LAL+ +AP+ SE ++P R+K+ LA M+T A+AP L
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 PPAIPPGSYAGLWLLGQQVLIGIAMGFTMRIVFAAVQTAGEFVGLQMGLSFASFFDPSTG 120
P S+ LWL QQ+LIGIA+GFTM+ FAAV+TAGE +GLQMGLSFA+F DP++
Sbjct: 61 NDV-PVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASH 119

Query: 121 ANTAVLSRLLNIVAMLVFLALDGHLLVLAALVRSFDVLPLTQLTLDPNGWGILVQWGQTI 180
N VL+R+++++A+L+FL +GHL +++ LV +F LP+ L+ N + L + G I
Sbjct: 120 LNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLI 179

Query: 181 FVSGLLLALPLICALLTINLAMGILNRAAPQLSVFAVGFPVSLITGLLLLAAVLPHAAPF 240
F++GL+LALPLI LLT+NLA+G+LNR APQLS+F +GFP++L G+ L+AA++P APF
Sbjct: 180 FLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPF 239

Query: 241 LEGLMRDGLQAISDVL 256
E L + ++D++
Sbjct: 240 CEHLFSEIFNLLADII 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2136TYPE3IMQPROT591e-15 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 59.4 bits (144), Expect = 1e-15
Identities = 26/78 (33%), Positives = 44/78 (56%)

Query: 4 ETVMSMTYQALKIALAMAGPLLLVTLAVGLVIAVFQAATQINEMTLSFIPKLLAMCGVLV 63
+ ++ +AL + L ++G +V +GL++ +FQ TQ+ E TL F KLL +C L
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 LMGPWLLGLMTDYIRQLI 81
L+ W ++ Y RQ+I
Sbjct: 62 LLSGWYGEVLLSYGRQVI 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2137FLGBIOSNFLIP2837e-99 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 283 bits (725), Expect = 7e-99
Identities = 162/243 (66%), Positives = 190/243 (78%), Gaps = 2/243 (0%)

Query: 19 LAAAALAGLALFPAGVVAQATLPALTATPGPGGAQTYSLSMQTLLLMTSLSFLPAALLMM 78
L + A L L AQ LP +T+ P PGG Q++SL +QTL+ +TSL+F+PA LLMM
Sbjct: 4 LLSVAPVLLWLITPLAFAQ--LPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMM 61

Query: 79 TGFTRIIIVLGLLRSALGTAMSPPNHVLIGLALFLTFYTMSPVFDRIYSEAYKPLSEGSI 138
T FTRIIIV GLLR+ALGT +PPN VL+GLALFLTF+ MSPV D+IY +AY+P SE I
Sbjct: 62 TSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKI 121

Query: 139 PFETAVERAAAPLHTFMLHQTRENDLTLFANLANQPALEDPSQVPMKILVPAFITSELKT 198
+ A+E+ A PL FML QTRE DL LFA LAN L+ P VPM+IL+PA++TSELKT
Sbjct: 122 SMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKT 181

Query: 199 AFQIGFTIFIPFLIIDLVVASVLMALGMMMVPPVTVALPFKLMLFVLADGWNLLLGSLAS 258
AFQIGFTIFIPFLIIDLV+ASVLMALGMMMVPP T+ALPFKLMLFVL DGW LL+GSLA
Sbjct: 182 AFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQ 241

Query: 259 SFY 261
SFY
Sbjct: 242 SFY 244


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2139FLGMOTORFLIN1365e-44 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 136 bits (345), Expect = 5e-44
Identities = 78/133 (58%), Positives = 94/133 (70%), Gaps = 13/133 (9%)

Query: 46 DDWAGAMAEQASAASTAPAAAAPAAAPAARPAGGSVFKPLADAAGGNGNDIDLIMDVPVQ 105
D WA A+ EQ + + + A A GG V + D IDLIMD+PV+
Sbjct: 17 DLWADALNEQKATTTKSAADAVFQQL-----GGGDVSGAMQD--------IDLIMDIPVK 63

Query: 106 LTVELGRTRLTIKNLLQLGQGSVVELDGLAGEPMDIFVNGYLIAQGEVVVVEEKYGIRLT 165
LTVELGRTR+TIK LL+L QGSVV LDGLAGEP+DI +NGYLIAQGEVVVV +KYG+R+T
Sbjct: 64 LTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRIT 123

Query: 166 DIITPSERINRLN 178
DIITPSER+ RL+
Sbjct: 124 DIITPSERMRRLS 136


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2140FLGMOTORFLIM2782e-94 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 278 bits (712), Expect = 2e-94
Identities = 97/318 (30%), Positives = 165/318 (51%), Gaps = 8/318 (2%)

Query: 7 LSQDEVDALLAGV-TGESDSE-SRDEADARGARAYDLSSPDRVVRRRMQTLELINERFAR 64
LSQDE+D LL + +G++ E +R +D R YD PD+ + +M+TL L++E FAR
Sbjct: 5 LSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFAR 64

Query: 65 QLRHLLLNFMRRNADITVGSIKILKYADFERNLPVPSNLNMIQMKPLRGTALFTYDPSLV 124
L +R + V S+ L Y +F R++P PS L +I M PL+G A+ DPS+
Sbjct: 65 LTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPSIT 124

Query: 125 FLVIDSLFGGDGRYHTRVEGRDFTTTEQRIIRRLLNLTLESYGKSWEAVYPIEFEYVRSE 184
F +ID LFGG G+ RD T E ++ ++ L + +SW V + + E
Sbjct: 125 FSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQIE 182

Query: 185 MHTKFASITGNNEVVVVSSFHIEFGATGGDLNICLPYSMIEPVRDLL-TRPLQETTLEEV 243
+ +FA I +E+VV+ + + G G +N C+PY IEP+ L ++ +
Sbjct: 183 TNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRRSS 242

Query: 244 DQRWTHQLSRQVRSADVDLTAEFASIPSSIRELLRLKVGDVLPIE---VPETVIANVNGV 300
++ L ++ + D+D+ AE S+ S+R++L L+VGD++ + V + + ++
Sbjct: 243 TTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIGNR 302

Query: 301 PLMECSYGVFNGQYALRV 318
C GV + A ++
Sbjct: 303 KKFLCQPGVVGKKIAAQI 320


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2142FLGHOOKFLIK577e-11 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 56.8 bits (136), Expect = 7e-11
Identities = 72/280 (25%), Positives = 99/280 (35%), Gaps = 10/280 (3%)

Query: 173 PPAAALALSAAAPANTPAPQTQAPAAARPDTRVPGHELRGAPAPMPNPNAVAVTAVAEAP 232
P A ++ AA A+ + + D L N V P
Sbjct: 97 PLTTAQTMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLP 156

Query: 233 EHLNAQAAAEAELALQAASAVAQPAGHGAASSHAADAAASLAAAASPQATAPAPMPQAGA 292
L A P + A S A S + A
Sbjct: 157 TEKPTLFTKLTSEQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLIT 216

Query: 293 LSLAVATPVAATPAWGADLGRQLVVLSHD------ATRGQHTAELRLDPPDLGPLRVTLS 346
P A P A LG S +GQ +AELRL P DLG ++++L
Sbjct: 217 PHQTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK 276

Query: 347 VNDGVASASFVSAHAAVRHAVEAALPQLHQALAQAGLSLGQANVGEH---GSQSGFDMQQ 403
V+D A VS H VR A+EAALP L LA++G+ LGQ+N+ G Q QQ
Sbjct: 277 VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQ 336

Query: 404 QAQGGGHGQGGGTQGDGAVALAPAAATRVARGDGLVDTFA 443
Q+Q + + + D + P + G+ VD FA
Sbjct: 337 QSQRTANHEPLAGEDDDTLP-VPVSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2143FLGFLIJ673e-17 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 67.1 bits (163), Expect = 3e-17
Identities = 51/145 (35%), Positives = 76/145 (52%)

Query: 1 MPSQLPLDTLIGLARESTDEAARALGRLNAERSHAERQLSMLQDYRQDYLLRLQNAMQTG 60
M L TL LA + ++AAR LG + AE QL ML DY+ +Y L + M G
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 MSAADCHNYQRFIATLDDAIGQQAAVLRQADSHLAQGRVHWQQQQRRLNSFDALAERERR 120
+++ NYQ+FI TL+ AI Q L Q + W+++++RL ++ L ER+
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 AQAVLETRREQRASDEFASRMMFRQ 145
A + E R +Q+ DEFA R R+
Sbjct: 121 AALLAENRLDQKKMDEFAQRAAMRK 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2145FLGFLIH912e-24 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 91.4 bits (226), Expect = 2e-24
Identities = 60/219 (27%), Positives = 105/219 (47%), Gaps = 6/219 (2%)

Query: 23 WRRWQMSSFDLPVEDAIEIVAPPPEPDPGPDPEELLREARAQAEAAGRREGLQQGREQGL 82
W+ W P + + IV P E + E L + AQ + + +QG + G+
Sbjct: 7 WKTWTPDDLAPPQAEFVPIVEP--EETIIEEAEPSLEQQLAQLQM----QAHEQGYQAGI 60

Query: 83 REGRQTGHAEGLAAGREAGYQEGLTQGREQARQEALQLHALAESCGASLADLEARMGQAL 142
EGRQ GH +G G G ++GL + + Q ++ L +L L++ + L
Sbjct: 61 AEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRL 120

Query: 143 LTLALDIAGQILRTTLAEQPESMLAAVREVLHINPAATGAMRLWVHPADLELVRQHLADE 202
+ +AL+ A Q++ T +++ ++++L P +G +L VHP DL+ V L
Sbjct: 121 MQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT 180

Query: 203 LREGHWRVLADESIARGGCRAETPYGDIDATLQTRWRRI 241
L WR+ D ++ GGC+ GD+DA++ TRW+ +
Sbjct: 181 LSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2146FLGMOTORFLIG295e-101 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 295 bits (757), Expect = e-101
Identities = 114/333 (34%), Positives = 189/333 (56%), Gaps = 2/333 (0%)

Query: 2 KNDGKPLDGVTRSAVLMMSLGEDAAAEVFKYLSAREVQLVGGSMANLKQVTRGDVAVVLE 61
D L G ++A+L++S+G + +++VFKYLS E++ + +A L+ +T VL
Sbjct: 9 ILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLL 68

Query: 62 EFRQEADQFMAVTLGSDDYIRTVLTKALGSDRAAGLIEDILEAGEGASGIDALNWLDPHT 121
EF++ + G DY R +L K+LG+ +A +I + L + + + + DP
Sbjct: 69 EFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINN-LGSALQSRPFEFVRRADPAN 127

Query: 122 VAELIGDEHPQIIATILVHLERDRAAGVLALLTDRLRNDVMLRIATFGGVQPAALSELTD 181
+ I EHPQ IA IL +L+ +A+ +L+ L ++ +V RIA P + E+
Sbjct: 128 ILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVER 187

Query: 182 VLNSVLAGQGA-KRSKMGGVRTAAEILNMMSSAEEEAVVESLRERDSDLAQKIIDEMFVF 240
VL LA + + GGV EI+NM E+ ++ESL E D +LA++I +MFVF
Sbjct: 188 VLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMFVF 247

Query: 241 DNLIDVEDRALQLILKEIDNDSLMVALKGASEELRNKFLRNMSSRAADILREDLEAQGPI 300
++++ ++DR++Q +L+EID L ALK ++ K +NMS RAA +L+ED+E GP
Sbjct: 248 EDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLGPT 307

Query: 301 RMSKVESEQKKILQIARRLAESGQIVLGNQGDD 333
R VE Q+KI+ + R+L E G+IV+ G++
Sbjct: 308 RRKDVEESQQKIVSLIRKLEEQGEIVISRGGEE 340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2147FLGMRINGFLIF452e-156 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 452 bits (1165), Expect = e-156
Identities = 244/555 (43%), Positives = 351/555 (63%), Gaps = 24/555 (4%)

Query: 18 LEKVRALPKPVLLGVAAALVAIVAVLAMWGREPDYKVLFANLDDRDGGAIVSALGQMNVP 77
L ++RA P+ L+ +A VAIV + +W + PDY+ LF+NL D+DGGAIV+ L QMN+P
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 78 YRFSGDGRALLVPADRVYATRMQLAGQGLPRGGSVGFELLDNARFGASQFAEQINYQRGL 137
YRF+ A+ VPAD+V+ R++LA QGLP+GG+VGFELLD +FG SQF+EQ+NYQR L
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRAL 135

Query: 138 EGELARSIEAMNTVQSARVHLALPRQSLFVRDRQAPTASVLLHLYPGRSLGDAQVAAVAW 197
EGELAR+IE + V+SARVHLA+P+ SLFVR++++P+ASV + L PGR+L + Q++AV
Sbjct: 136 EGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVH 195

Query: 198 LVASSVPDLTAENISIVDQNGRLLSAPLGEGRGLDADQSRLRRDIEQRTVERILTILNPL 257
LV+S+V L N+++VDQ+G LL+ GR L+ Q + D+E R RI IL+P+
Sbjct: 196 LVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDVESRIQRRIEAILSPI 255

Query: 258 VGPGNVQAQASAEMDFARREQTSEVYRPNQEPGQAAVRSKQTSDSLQTGIDPAQGVPGAL 317
VG GNV AQ +A++DFA +EQT E Y PN + +A +RS+Q + S Q G GVPGAL
Sbjct: 256 VGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGAL 315

Query: 318 SNQPPAAAQAPIVNPPAAPQAAQGGQPGQLAQAGQNAAQGAATQAAPRLPTNNRNDATIN 377
SNQP +API PP Q AQ + +A P + + + T N
Sbjct: 316 SNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAG-----------PRSTQRNETSN 364

Query: 378 YEVDRTISHVKQPVGMLKRLSVAVVVNYLPDSSGEPQPLPEEELTKLTNLVREAMGYSEA 437
YEVDRTI H K VG ++RLSVAVVVNY + G+P PL +++ ++ +L REAMG+S+
Sbjct: 365 YEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAMGFSDK 424

Query: 438 RGDSLNLVNSQFN---DKPVKPPFWRDPELLDLVKTVLAWVFGLALALWLYRR-LRPAVS 493
RGD+LN+VNS F+ + + PFW+ +D + W+ L +A L+R+ +RP ++
Sbjct: 425 RGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVRPQLT 484

Query: 494 NYL-NPPVDPEEAEARRQEMQREAQAAA--------RAKEVNRYEDNLQRARDMATKDPR 544
+ E+A+ R++ + + RA + E QR R+M+ DPR
Sbjct: 485 RRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPR 544

Query: 545 AVAMVMRAWMTQDEK 559
VA+V+R WM+ D +
Sbjct: 545 VVALVIRQWMSNDHE 559


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2148FLGHOOKFLIE618e-16 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 61.2 bits (148), Expect = 8e-16
Identities = 49/108 (45%), Positives = 72/108 (66%), Gaps = 6/108 (5%)

Query: 4 SGLSGIESMLQQMRAVVQAAQSNGVSPAELAPQPA-SFAAELQRSLQRVSAAQIAATNQG 62
S + GIE ++ Q++A +A++ E PQP SFA +L +L R+S Q AA Q
Sbjct: 1 SAIQGIEGVISQLQATAMSARAQ-----ESLPQPTISFAGQLHAALDRISDTQTAARTQA 55

Query: 63 KAYELGAPGVSLNDVMIDLQKSSIAFQTAVQVRNRLVAAYKEISAMSV 110
+ + LG PGV+LNDVM D+QK+S++ Q +QVRN+LVAAY+E+ +M V
Sbjct: 56 EKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2150TYPE3IMSPROT769e-20 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 75.6 bits (186), Expect = 9e-20
Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 2/79 (2%)

Query: 16 AVALSYGEHDT-APRVVAKGYGQIADTIVRTAREHGLYVHESRELV-SLLMQVDLDAHIP 73
A+ + Y +T P V K T+ + A E G+ + + L +L +D +IP
Sbjct: 268 AIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDHYIP 327

Query: 74 PQLYAAVAELLAWLYRLET 92
+ A AE+L WL R
Sbjct: 328 AEQIEATAEVLRWLERQNI 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2151PF03544310.010 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 30.7 bits (69), Expect = 0.010
Identities = 21/93 (22%), Positives = 27/93 (29%), Gaps = 2/93 (2%)

Query: 119 QAPPAQGKAPLWQPAPPPGPSAAAPDAGNSVRPAPAAA--GTASSSAANPATDPAAASSR 176
Q PP P +P P P P AP +P P P +
Sbjct: 67 QPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPA 126

Query: 177 SPATGGRPALPAGAGAQATQATQATHAGAPALP 209
SP PA P + A A + T +
Sbjct: 127 SPFENTAPARPTSSTATAATSKPVTSVASGPRA 159


89Bpet2290Bpet2302N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2290028-4.864815ATP-dependent DNA helicase RecG
Bpet2291142-9.166139LysR family transcriptional regulator
Bpet2292152-11.113146putative DNA-binding protein
Bpet2293157-12.398263hypothetical protein
Bpet2294257-13.273892short-chain sugar nucleotide oxidoreductase
Bpet2295360-15.048748sulfatase involved in polysaccharide
Bpet2296462-16.001161MPA2 family protein involved in capsular
Bpet2297557-15.430246outer membrane protein involved in
Bpet2298454-14.957342permease component of an ABC exporter involved
Bpet2299352-13.920615polysaccharide ABC transporter ATP-binding
Bpet2300249-12.222365hypothetical protein
Bpet2301037-8.447659hypothetical protein
Bpet2302-132-7.049983sugar nucleotide epimerase / oxidoreductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2290SECA310.026 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 30.6 bits (69), Expect = 0.026
Identities = 18/77 (23%), Positives = 32/77 (41%), Gaps = 5/77 (6%)

Query: 294 RLLQGDV-----GSGKTVVAAIAAAQAIACGAQVALMAPTEILAEQHFRKLVSWLQPLGV 348
L + + G GKT+ A + A G V ++ + LA++ + LG+
Sbjct: 93 VLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGL 152

Query: 349 NVAWLSGSLTAKARRQA 365
V + A A+R+A
Sbjct: 153 TVGINLPGMPAPAKREA 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2292HELNAPAPROT1432e-46 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 143 bits (361), Expect = 2e-46
Identities = 50/144 (34%), Positives = 76/144 (52%)

Query: 23 DRTAIAGELSKVLADSYTLYLMTHNFHWNVTGPLFNTLHQMFMTQYSEEWAALDDIAERI 82
++T + L+ L++ + LY H FHW V GP F TLH+ F Y +D IAER+
Sbjct: 9 NQTLVENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERL 68

Query: 83 RALGVHAPGTYREFSKLSSISEPGAVPDAMEMVRLLVKGNEAVSKTARAAFDKADSANDQ 142
A+G T +E+++ +SI++ G A EMV+ LV + +S ++ A+ D
Sbjct: 69 LAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYKQISSESKFVIGLAEENQDN 128

Query: 143 PTADLLTQRMDIHEKNAWMLRSLL 166
TADL ++ EK WML S L
Sbjct: 129 ATADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2294DHBDHDRGNASE747e-18 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 74.3 bits (182), Expect = 7e-18
Identities = 56/211 (26%), Positives = 85/211 (40%), Gaps = 13/211 (6%)

Query: 2 LITGATGSIGGALALEYAKAGVDTLILQGRRTERLAELAQLCRREGAQVETHALDVRDHA 61
ITGA IG A+A A G + E+L ++ + E E DVRD A
Sbjct: 12 FITGAAQGIGEAVARTLASQGAHIAAVD-YNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70

Query: 62 SLIAWLTQICEVHAPDLVIVNAGININVGSDRQGEIWQDVHELLDVNVKAAFATVHGVLP 121
++ +I P ++VN + G ++ VN F V
Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSD-EEWEATFSVNSTGVFNASRSVSK 129

Query: 122 FMRKRGQGQIALVSSLAAWRGLPETP--SYSASKAAIKVYGEAMRDGLAAEGIRFNVIMP 179
+M R G I V S A G+P T +Y++SKAA ++ + + LA IR N++ P
Sbjct: 130 YMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 180 GYVESPMCFDMPGPKPFLWTAARAAHAIRRG 210
G E+ M + LW A + +G
Sbjct: 188 GSTETDM-------QWSLWADENGAEQVIKG 211


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2296RTXTOXIND310.006 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.006
Identities = 21/175 (12%), Positives = 56/175 (32%), Gaps = 12/175 (6%)

Query: 150 AQQTLDIMLQESERFVNELSHRMAREQMNFAKSELANARRAYEERREALLTFQSANSLLD 209
Q I+ + E N+L ++ F R +E T+Q+
Sbjct: 149 EQTRYQILSRSIEL--NKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQ--KYQ 204

Query: 210 AEAAAKARAEVISELEASLTKERTTLKGLLATLDSNTPQVRQQ---RNRIQAMEQQLAAE 266
E + + A + + + + LD + + +Q ++ + E +
Sbjct: 205 KELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEA 264

Query: 267 TRRLVSQQGGDKLNVVASQYRNLTIDAAIAEEAYKFAVSSVETARIEASKKLRSL 321
L + +L + S+ + + + + +K + + + + + L
Sbjct: 265 VNELRVYKS--QLEQIESEILSAKEEYQLVTQLFK---NEILDKLRQTTDNIGLL 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2298ABC2TRNSPORT369e-05 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 36.1 bits (83), Expect = 9e-05
Identities = 48/197 (24%), Positives = 86/197 (43%), Gaps = 15/197 (7%)

Query: 37 LFEPIAHITFLMFLMTVVRGRHLPGFDYPIYLLTGLVPFFLMRNISLKMMEA----INAN 92
L EP+ ++ L + V+ GR + G Y +L G+V M + + + A +
Sbjct: 39 LAEPLIYLFGLGAGLGVMVGR-VGGVSYTAFLAAGMVATSAMTAATFETIYAAFGRMEGQ 97

Query: 93 RPLFA--YPNIKPFDTFLARLI---VECSLSACIYVLLLCAMGFWLGYDISIHAPLSWFV 147
R A Y ++ D L + + +L+ ++ A+G+ + P V
Sbjct: 98 RTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWLSLLYALP----V 153

Query: 148 ALLTGIAFAFGLGLVLCVVGEAMPNSKTFIRLMFLPLYLISGVIFPIWILPIRYMEWLLW 207
LTG+AFA LG+V+ + + + L+ P+ +SG +FP+ LPI + +
Sbjct: 154 IALTGLAFA-SLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQTAARF 212

Query: 208 NPYLHIIDNLRYSVFEH 224
P H ID +R + H
Sbjct: 213 LPLSHSIDLIRPIMLGH 229


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2299PF05272280.025 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.025
Identities = 12/20 (60%), Positives = 13/20 (65%)

Query: 37 LIGRNGAGKSTLMRLLGGLD 56
L G G GKSTL+ L GLD
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2302NUCEPIMERASE797e-19 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 79.4 bits (196), Expect = 7e-19
Identities = 40/195 (20%), Positives = 76/195 (38%), Gaps = 36/195 (18%)

Query: 6 TILITGGTGSFGNTFVPMTLAKY---NPKKVIIFSRDEMKQ-WDMARKFH-----DDPRV 56
L+TG G F+ ++K +V+ D + +D++ K P
Sbjct: 2 KYLVTGAAG-----FIGFHVSKRLLEAGHQVVGI--DNLNDYYDVSLKQARLELLAQPGF 54

Query: 57 RFFIGDVRDRERLYRALD--GVDYVVHAAATKIVPTAEYNPFECVKTNVDGAMNLIDACI 114
+F D+ DRE + + V + V + NP +N+ G +N+++ C
Sbjct: 55 QFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCR 114

Query: 115 DKGVKRVVALST---------------DKASSPINLYGATKLASDKLFVAGNAYSGEHGT 159
++ ++ S+ D P++LY ATK A++ + + YS +G
Sbjct: 115 HNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELM---AHTYSHLYGL 171

Query: 160 RFAVVRYGNVMGSRG 174
+R+ V G G
Sbjct: 172 PATGLRFFTVYGPWG 186


90Bpet2374Bpet2380N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2374-1100.664697putative transcriptional regulatory protein
Bpet2375-2111.550800sigma-54 dependent DNA-binding response
Bpet2376-1122.359930thiamine pyrophosphate protein
Bpet2377-1112.369944hypothetical protein
Bpet2378-1112.310437hypothetical protein
Bpet2379-1122.835232hypothetical protein
Bpet23800133.323601ABC transporter, ATP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2374HTHFIS324e-110 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 324 bits (833), Expect = e-110
Identities = 117/343 (34%), Positives = 174/343 (50%), Gaps = 31/343 (9%)

Query: 52 GMCPLMREFGSQVARAARSDATVFITGESGTGKEMIARAIHEGSERSKHPFIPVNCGAFS 111
G M+E +AR ++D T+ ITGESGTGKE++ARA+H+ +R PF+ +N A
Sbjct: 141 GRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIP 200

Query: 112 HSLAHAQLFGHEKGSFTGALGQTAGYFESAGNGTLFLDEVTEMSDALQVQFLRVLESGTY 171
L ++LFGHEKG+FTGA ++ G FE A GTLFLDE+ +M Q + LRVL+ G Y
Sbjct: 201 RDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEY 260

Query: 172 QRVGGTEVLHTGARIVCATNRDPYAAVESGKLRQDFLHRLLIVPLRVPPLREREGDVRIL 231
VGG + + RIV ATN+D ++ G R+D +RL +VPLR+PPLR+R D+ L
Sbjct: 261 TTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDL 320

Query: 232 AQRFLDELNAAHKTSKRFSARMMDALLSYDWPGNVRELRNAVQRAFIMAD---------- 281
+ F+ + KRF ++ + ++ WPGNVREL N V+R +
Sbjct: 321 VRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITREIIE 380

Query: 282 TVVEAEFRRRPRAAEARDSTDGALCFPVGTPLSQA---------------------QRDV 320
+ +E P A S ++ V + Q + +
Sbjct: 381 NELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPL 440

Query: 321 ILATLAHHNGDKQRTADTLGVSLKTLYNRLGAYNADTPTSTRQ 363
ILA L G++ + AD LG++ TL ++ S+R
Sbjct: 441 ILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSSRS 483


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2375HTHFIS464e-163 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 464 bits (1195), Expect = e-163
Identities = 167/471 (35%), Positives = 252/471 (53%), Gaps = 34/471 (7%)

Query: 2 PHMLVLDDDEAVREVLAEIAREHGFSVAQAATMKDAMIQLQRQQPDLVLTDVRLPGASGM 61
+LV DDD A+R VL + G+ V + + DLV+TDV +P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 EIFGRMQ--NSDAEVVVITGHGTMDNAVEALRLGATDYLVKPVCMDRLAEILTRVAADRG 119
++ R++ D V+V++ T A++A GA DYL KP + L I+ R A+
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 120 GAANDGPFQEPGRFGKLLGQSESMEQLYAQLSRVAATDATTLLIGESGTGKELAAHAIHE 179
+ + L+G+S +M+++Y L+R+ TD T ++ GESGTGKEL A A+H+
Sbjct: 124 RRPSKLE-DDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHD 182

Query: 180 LSSRASKPFIAVNCGAISPHLIESELFGHERGSFTGADRQHKGYFERADSGTLFLDEVTE 239
R + PF+A+N AI LIESELFGHE+G+FTGA + G FE+A+ GTLFLDE+ +
Sbjct: 183 YGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGD 242

Query: 240 MPLDLQVKLLRVLETGRFMRVGTHREVACDVRIVAATNRSPEQAIQEGKLREDLYYRLSV 299
MP+D Q +LLRVL+ G + VG + DVRIVAATN+ +Q+I +G REDLYYRL+V
Sbjct: 243 MPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNV 302

Query: 300 FPIELPPLRERGTDILFLADRFLQALNDKYGESRRFSEQARQAIGEYAWPGNVRELKNYV 359
P+ LPPLR+R DI L F+Q + + +RF ++A + + + WPGNVREL+N V
Sbjct: 303 VPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 360 RRAYIMAEDGILGADALQPQIAPD-------------------------------GGGQA 388
RR + ++ + ++ ++ + G A
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 389 ARVVVPLGVTLAEADRRLIMATLERCGGVKKQTAAVLGISAKTLYNRLEEY 439
LAE + LI+A L G + + A +LG++ TL ++ E
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIREL 473


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2377PF05616280.005 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 27.8 bits (61), Expect = 0.005
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 5/58 (8%)

Query: 8 DAAPGRAQTYPPKEPVQDPPVRPDDTPEDTPRKPYPPDSIPNPM--PGLDPQQTPGID 63
D PG A+ P +P+ P V P + P + P P + PNP P L+P P D
Sbjct: 314 DLTPGSAEA-PNAQPL--PEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTD 368


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2380HTHFIS732e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 73.3 bits (180), Expect = 2e-15
Identities = 31/134 (23%), Positives = 58/134 (43%), Gaps = 9/134 (6%)

Query: 760 LSGVAIMVADDQEDARGLVAEVLADRGAAVHTCASGADVLAALRQASWPDLLVCDISLGD 819
++G I+VADD R ++ + L+ G V ++ A + + DL+V D+ + D
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAG-DGDLVVTDVVMPD 59

Query: 820 MEGYELIGRIRALEAERGAPLGERMPAVALSGHTGPEDRLRALLAGFQIHVAKPVDPREL 879
++L+ RI+ +P + +S ++A G ++ KP D EL
Sbjct: 60 ENAFDLLPRIKK--------ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTEL 111

Query: 880 LATVSAMLRPDTRR 893
+ + L RR
Sbjct: 112 IGIIGRALAEPKRR 125


91Bpet2590Bpet2594N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet25901144.353378TetR family transcriptional regulator
Bpet25911143.937586HlyD family secretion protein
Bpet25920143.100304putative ATP-binding component of a transport
Bpet25931134.024424ABC-type multidrug transport system, permease
Bpet25941114.605066outer membrane exporter protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2590HTHTETR692e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 68.9 bits (168), Expect = 2e-16
Identities = 28/180 (15%), Positives = 73/180 (40%), Gaps = 15/180 (8%)

Query: 12 LGRPARPQRADSRDAMLDVATALFAAQGVAATTIAHIARRADVTPAMVHYYFKNREQLID 71
+ R + + ++R +LDVA LF+ QGV++T++ IA+ A VT ++++FK++ L
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 72 VVVAERLAPVIASVWAPAALPAGNGPGAAPPTPPEPRAMVAQVVARIMQCAAERP----W 127
+ + + P +P +++ +++ +++
Sbjct: 61 EIWELSESNIGELE-----------LEYQAKFPGDPLSVLREILIHVLESTVTEERRRLL 109

Query: 128 LAPLWMREVVNEGGQLREKVFRYLPVERLHAFAATITSAQQQGAVNPGIEPRLVFLSILG 187
+ ++ + + ++ R L +E T+ + + + R + + G
Sbjct: 110 MEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRG 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2591RTXTOXIND506e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 50.2 bits (120), Expect = 6e-09
Identities = 37/280 (13%), Positives = 82/280 (29%), Gaps = 25/280 (8%)

Query: 64 ARGQQVQAGAPLFALEADPEAQAQREARARLASAQAQRQDLATGKRAPEVDVVRAQLAQA 123
++V L + + + L +A+R + E +
Sbjct: 179 VSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLD 238

Query: 124 EAEAKRAAAQLARDRVQFQAGGIARAQLDDSRAQAQSSAARVRELRAQLQVAGLPGR--- 180
+ + +A+ V Q A + ++Q L A+ + +
Sbjct: 239 DFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKN 298

Query: 181 --DEQLRAQDAQVEAARAGLAQADWALAQKQVAAAQAARVFD-TLYRVGEWVPAGSPVVR 237
++LR + LA+ + + A + +V ++ G V ++
Sbjct: 299 EILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMV 358

Query: 238 LLPPGN-IKLRFFVPETALGGLRSGQAVRARCDACGE----PVAATISYIAAEAEYTPPV 292
++P + +++ V +G + GQ + +A + + I +A
Sbjct: 359 IVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA------ 412

Query: 293 IYSRDSRGKLVYMV------EAHPAPRDATRLHPGQPVEV 326
D R LV+ V L G V
Sbjct: 413 --IEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTA 450


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2592BACINVASINB290.027 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 29.0 bits (64), Expect = 0.027
Identities = 12/38 (31%), Positives = 24/38 (63%)

Query: 128 QAVEQALEGLGLQSRANQLTGSLSGGWKQRLALAACLL 165
+A+ +ALEGLG+ + ++ GS+ G +A+ A ++
Sbjct: 387 KAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMVAVIV 424


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2593ABC2TRNSPORT512e-09 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 50.7 bits (121), Expect = 2e-09
Identities = 42/173 (24%), Positives = 75/173 (43%), Gaps = 2/173 (1%)

Query: 208 AMTRERERGTMENLLATPVRPLEVMTGKIVPYIAIGLIQATIILLAALYVFHVPLMGSLL 267
A R + T E +L T +R +++ G++ + I + A + + + SLL
Sbjct: 90 AFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWL-SLL 148

Query: 268 AVYLAALLFVAANLTVGITLSSLAQNQLQAMQLTMFYFLPNILLSGFMFPFQGMPVWAQH 327
L A ++G+ +++LA + + P + LSG +FP +P+ Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 328 IGNLLPLTYFNRLIRGILLKGNGWADLWPHVWPLLLFTALIMALAVKFYRRTL 380
LPL++ LIR I+L D+ HV L ++ + L+ RR L
Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPV-VDVCQHVGALCIYIVIPFFLSTALLRRRL 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2594RTXTOXIND310.012 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.0 bits (70), Expect = 0.012
Identities = 21/184 (11%), Positives = 49/184 (26%), Gaps = 27/184 (14%)

Query: 302 PLGVPSQLTRQRPDILAAEALWHRAAADVGVATANLYPRFTLTGSFGSQRTRAGDVADGV 361
LG + + + +L A R N P L Q +V
Sbjct: 129 ALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVL--- 185

Query: 362 NVWSLALGLTQPLFHGGELRARRRAAEAAYQAAAAAYRDTVLQGLQQVADALSAVQADAD 421
L + + + +Q L + V A +
Sbjct: 186 -----------------RLTSLIKEQFSTWQNQKYQKE----LNLDKKRAERLTVLARIN 224

Query: 422 TLQARAEAERQAEAAYRITAQQYQAGGVSQLALLDAQREQLRTRAERIQAQADRHADTAA 481
+ + E+ + + +++ A+L+ + + + E ++ +
Sbjct: 225 RYENLSRVEKSRLDDFSSLLHK---QAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESE 281

Query: 482 LLQA 485
+L A
Sbjct: 282 ILSA 285



Score = 30.2 bits (68), Expect = 0.024
Identities = 16/116 (13%), Positives = 38/116 (32%), Gaps = 6/116 (5%)

Query: 372 QPLFHGGELRAR--RRAAEAAY-QAAAAAYRDTVLQGLQQVADALSAVQADADTLQARAE 428
L L A +++ QA R +L ++ D Q +E
Sbjct: 122 DVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSE 181

Query: 429 AERQAEAAYRITAQQYQAGGVSQLALLDAQREQLRTRAERIQAQADRHADTAALLQ 484
E + +Q+ +Q + ++ R + A+ +R+ + + + +
Sbjct: 182 EEVLRLTSLI--KEQFSTWQ-NQKYQKELNLDKKRAERLTVLARINRYENLSRVEK 234


92Bpet2644Bpet2651N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2644-1154.607223putative chelatase
Bpet2645-3172.248342hypothetical protein
Bpet2646-3192.698910nitrogen regulatory protein P-II
Bpet2647-2202.543002ammonium transporter
Bpet26480182.174678TetR family transcriptional regulator
Bpet2649-1181.150164putative transmembrane transport protein
Bpet2650-1171.810655putative branched-chain amino acid ABC
Bpet2651-1172.165770putative ABC transport protein, ATP-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2644HTHFIS310.009 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.3 bits (71), Expect = 0.009
Identities = 48/245 (19%), Positives = 77/245 (31%), Gaps = 36/245 (14%)

Query: 127 PVGAPLALALAVAREQPDATLVLPADSATVAAWVPGLQVLAAGA---------LAEVAAH 177
P L + + +PD +++ + T + + GA L E+
Sbjct: 58 PDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASE---KGAYDYLPKPFDLTELIGI 114

Query: 178 LSGAAPLPRAEPGAWPAAAASPCLSDVRGQPM--ARRALEVAAAGAHSLLMVGPPGAGKS 235
+ A P+ P + R M R L +L++ G G GK
Sbjct: 115 IGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKE 174

Query: 236 MLAQRLPGLLPPLSRTQALEAAALAGLAGPAGMAAALQGQPPFRAPHHGASAAALVGGGA 295
++A+ L R A +A + + + L G H A G
Sbjct: 175 LVARALHDYGK--RRNGPFVAINMAAIPRDL-IESELFG--------HEKGAFT---GAQ 220

Query: 296 RPRPGEATLAHHGVLFLDELPEFDRRALEALREPLETG---RVAIARARHSVQYPARFQL 352
G A G LFLDE+ + A L L+ G V + ++
Sbjct: 221 TRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPI-----RSDVRI 275

Query: 353 VAAMN 357
VAA N
Sbjct: 276 VAATN 280


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2648HTHTETR535e-11 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 53.5 bits (128), Expect = 5e-11
Identities = 20/95 (21%), Positives = 38/95 (40%)

Query: 24 DRIRETARRMFYQDGIRAVGVDALVAEAGVTKPSLYRSFSSKDELAASYLRDYEAEFWSK 83
I + A R+F Q G+ + + + AGVT+ ++Y F K +L + E+
Sbjct: 14 QHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGEL 73

Query: 84 FEAGWQQHPDDPRAALMVYFGSLAQRAASSDGYRG 118
+ P DP + L + + + + R
Sbjct: 74 ELEYQAKFPGDPLSVLREILIHVLESTVTEERRRL 108


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2649TCRTETB362e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 36.4 bits (84), Expect = 2e-04
Identities = 26/134 (19%), Positives = 46/134 (34%), Gaps = 7/134 (5%)

Query: 24 VIFLALLVSAGLRSTP---SVLLVPLEESFGWSRATTSFSAAI---GIFLYGLVGPFAAA 77
+ F+ ++ G+ V +VP +T + I G + G
Sbjct: 256 IPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGI 315

Query: 78 AMERFGLRRVLIGALALMAASTFASSFMTEPWHLLLTWGV-FSGIGSGAVAVVLGATVVN 136
++R G VL + ++ S +SF+ E +T + F G V+ V +
Sbjct: 316 LVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSS 375

Query: 137 RWFATRRGLMMGLL 150
G M LL
Sbjct: 376 SLKQQEAGAGMSLL 389


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2651PF05272280.030 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.030
Identities = 11/21 (52%), Positives = 12/21 (57%)

Query: 35 LIGPNGAGKTTLFNVLTGLYI 55
L G G GK+TL N L GL
Sbjct: 601 LEGTGGIGKSTLINTLVGLDF 621


93Bpet2691Bpet2698N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2691-2132.413917putative sensor histidine kinase
Bpet2692-3132.633733two-component response regulatory protein
Bpet2693-2142.710680biopolymer transport protein ExbD/TolR
Bpet2694-2121.012310biopolymer transport ExbB protein
Bpet2695014-0.490571siderophore-mediated iron transport protein
Bpet2696217-1.131161hypothetical protein
Bpet2697217-1.344583two component system sensor kinase
Bpet2698318-2.743294two component system response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2691PF06580355e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 34.8 bits (80), Expect = 5e-04
Identities = 22/102 (21%), Positives = 40/102 (39%), Gaps = 22/102 (21%)

Query: 277 LIDNALRYG----QGGGRITLTVGLNPPSLT--VEDDGPGIPADEHERVFEAFYRSPGSM 330
L++N +++G GG+I L + ++T VE+ G + E
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE------------- 309

Query: 331 AGGSGLGLAIVRE-IAHAHGAWWKLSSRPEYPGTRLSVVFPG 371
+G GL VRE + +G ++ + V+ PG
Sbjct: 310 --STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIPG 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2692HTHFIS993e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 99.1 bits (247), Expect = 3e-26
Identities = 38/162 (23%), Positives = 76/162 (46%), Gaps = 7/162 (4%)

Query: 2 RVLVIEDDTTLGHALQEFLADQGYAVDWLTDGDKVLGALAGQSYDLLLLDLNLPGRSGLD 61
+LV +DD + L + L+ GY V ++ + +A DL++ D+ +P + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLRQLRQDGNQVPVLIVTARDGLEDRVAGLDAGADDYVTKPFDLPELAARVRAFGRRRAG 121
+L ++++ +PVL+++A++ + + GA DY+ KPFDL EL +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 122 QAQPLIEAGTLTFDTVG-----REVRANGQRLSLSVRELSVL 158
+ L + VG +E+ RL + +L+++
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQT--DLTLM 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2695TONBPROTEIN964e-26 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 96.2 bits (239), Expect = 4e-26
Identities = 56/165 (33%), Positives = 78/165 (47%), Gaps = 3/165 (1%)

Query: 84 EPEPEPEPEPEPVVEPEPEPVIEPEPEPEPPVIEKAPEPAPKPKPKPKPKPKPKPKPKIE 143
EP +P PEPVVEPEPEP PEP E PV+ + P+P PKPKPKP K + +PK ++
Sbjct: 56 EPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVK 115

Query: 144 KPVEPPKPVQPPSGAPEGAEVTQAPVQGPPPNEPIMVSSVEYLGRRPMPVYPMTSKRLRE 203
P P A +T + V+S R P YP ++ LR
Sbjct: 116 PVESRP---ASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRI 172

Query: 204 EGRVVVLVEINTQGLVERASIAQSSGYNRLDDSALAAARKARFKP 248
EG+V V ++ G V+ I + N + A R+ R++P
Sbjct: 173 EGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEP 217


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2698HTHFIS961e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 96.1 bits (239), Expect = 1e-25
Identities = 32/123 (26%), Positives = 63/123 (51%)

Query: 8 LLIDDDELYVRTLQRSLARHGLETRVATSIAEALRVAEDMLPSFALVDLRLGEDSGLTLI 67
L+ DDD L ++L+R G + R+ ++ A R + D+ + +++ L+
Sbjct: 7 LVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLL 66

Query: 68 RPLRALRADMRILLVTGYASVATAVEAIKRGADDYLPKPATAPMILRTLGLAKAESVAIE 127
++ R D+ +L+++ + TA++A ++GA DYLPKP ++ +G A AE
Sbjct: 67 PRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRP 126

Query: 128 STM 130
S +
Sbjct: 127 SKL 129



Score = 50.6 bits (121), Expect = 7e-10
Identities = 15/40 (37%), Positives = 24/40 (60%)

Query: 133 LHRLEWEHIQQALHECGGNVSAAARLLGMHRRSLQRKLAK 172
L +E+ I AL GN AA LLG++R +L++K+ +
Sbjct: 433 LAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRE 472


94Bpet2788Bpet2793N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet2788116-2.567276putative membrane protein (TRAP-type
Bpet2789418-2.667789ATP-dependent protease La
Bpet2790420-2.656606ATP-dependent protease ATP-binding subunit ClpX
Bpet2791217-1.959907ATP-dependent Clp protease proteolytic subunit
Bpet2792317-0.814300trigger factor
Bpet2793-1110.197766putative metalloprotease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2788PF03627300.019 PapG
		>PF03627#PapG

Length = 336

Score = 29.9 bits (67), Expect = 0.019
Identities = 15/47 (31%), Positives = 19/47 (40%), Gaps = 2/47 (4%)

Query: 87 HWPGGLATATLMSCGMFSTINGSSVATAATIGTVAIPE--MTQRGYN 131
W G+AT T C +GS I V P+ MT+ GY
Sbjct: 51 SWRPGIATVTWNQCNGPGFADGSWAYYREYIAWVVFPKKVMTKNGYP 97


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2789GPOSANCHOR330.007 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 32.7 bits (74), Expect = 0.007
Identities = 24/156 (15%), Positives = 55/156 (35%), Gaps = 14/156 (8%)

Query: 128 SETEALRRAIVAQFEQYVKLNKKIPPEILTSLAGIDDAGRLADTIAAHLPLKLEQKQKML 187
++ E + K + E A + + + + +
Sbjct: 228 ADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKT-- 285

Query: 188 EILGTSERLEGLLTQLETEIDILQVEKRIRGRVKKQMEKSQRDYYLNEQVKAIQKELGEG 247
+ LE LE + +L R +++ ++ S+ K ++ E +
Sbjct: 286 -LEAEKAALEAEKADLEHQSQVLNAN---RQSLRRDLDASREAK------KQLEAEHQKL 335

Query: 248 EEGADIEELEKKIIAAHM--PKEARKKADAELKKLK 281
EE I E ++ + + +EA+K+ +AE +KL+
Sbjct: 336 EEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLE 371


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2790HTHFIS290.042 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.042
Identities = 12/46 (26%), Positives = 23/46 (50%), Gaps = 3/46 (6%)

Query: 109 VELSKSNIMLIGPTGSGKTLLAQTL---ARMLNVPFVMADATTLTE 151
+ + +M+ G +G+GK L+A+ L + N PFV + +
Sbjct: 156 LMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPR 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2793ADHESNFAMILY290.020 Adhesin family signature.
		>ADHESNFAMILY#Adhesin family signature.

Length = 309

Score = 28.7 bits (64), Expect = 0.020
Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 1/56 (1%)

Query: 44 AEIKTLSDQACAAS-DKQEKVAKSGSKYDARLQKLAKALGTKVNGQPASYKVYITS 98
K ++ Q A + +E K+ +Y +L KL K K N PA K+ +TS
Sbjct: 149 IFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTS 204


95Bpet2856Bpet2859N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet28562154.716167general secretion pathway protein G
Bpet28571155.884963general secretion pathway protein H
Bpet28582156.491578general secretion pathway protein I
Bpet28592145.712154general secretion pathway protein J
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2856BCTERIALGSPG1688e-57 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 168 bits (427), Expect = 8e-57
Identities = 65/142 (45%), Positives = 89/142 (62%), Gaps = 8/142 (5%)

Query: 17 RPRARQQGFTLIEIMVVIVIMGILAALVVPRVLDRPDQARRVAARQDISGLMQALKLYRL 76
R +Q+GFTL+EIMVVIVI+G+LA+LVVP ++ ++A + A DI L AL +Y+L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKL 61

Query: 77 DNGRYPNAAQGLQALVRRP---DGARNWRP--YLDRLPDDPWGHPYQYLNPGVKGEIDVF 131
DN YP QGL++LV P A N+ Y+ RLP DPWG+ Y +NPG G D+
Sbjct: 62 DNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLL 121

Query: 132 TFGPDNKAGGEEDDADIGSWDL 153
+ GPD + G E+ DI +W L
Sbjct: 122 SAGPDGEMGTED---DITNWGL 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2857BCTERIALGSPH525e-11 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 51.9 bits (124), Expect = 5e-11
Identities = 17/90 (18%), Positives = 32/90 (35%)

Query: 8 ISERGFTLIEMLVVVAIIAIAASMVGLSVTSSSGRALRADAERLVDAFAVAQSEARSDGR 67
+ +RGFTL+EM++++ ++ ++A MV L+ +S + R Q G+
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQ 60

Query: 68 AILWRADERGWSFERRGRPARVSAQDDGPQ 97
W F
Sbjct: 61 FFGVSVHPDRWQFLVLEARDGADPAPADDG 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2858BCTERIALGSPG412e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 40.6 bits (95), Expect = 2e-07
Identities = 20/64 (31%), Positives = 39/64 (60%), Gaps = 3/64 (4%)

Query: 1 MPSSRQQRGFTLIEVLVALAIISVAMGAAMRATQVMLDNSRAIRDKTLALLAA-DNTLAR 59
M ++ +QRGFTL+E++V + II V A++ +M + +A + K ++ + A +N L
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVL--ASLVVPNLMGNKEKADKQKAVSDIVALENALDM 58

Query: 60 LRLE 63
+L+
Sbjct: 59 YKLD 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2859BCTERIALGSPG290.008 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 28.7 bits (64), Expect = 0.008
Identities = 9/27 (33%), Positives = 19/27 (70%)

Query: 5 RRCAPQQGFTLIEVLIAIALMALVSLL 31
R Q+GFTL+E+++ I ++ +++ L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASL 28


96Bpet2945Bpet2952N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet29450124.764720hypothetical protein
Bpet29463125.576961putative outer membrane proton channel
Bpet29471144.218614OmpA-family protein
Bpet29483153.655557hypothetical protein
Bpet29492163.214398TetR family transcriptional regulator
Bpet29501172.389926AraC family transcriptional regulator
Bpet29512142.817078hypothetical protein
Bpet29521171.924539putative ABC-transporter membrane-spanning
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2945IGASERPTASE280.009 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 28.5 bits (63), Expect = 0.009
Identities = 14/68 (20%), Positives = 26/68 (38%)

Query: 19 SPVAALAQAAPAAQPPTQAQPAIQPSEEQLQKFASASQKVAMVADEYRPKLQAAKDDAAR 78
+ VA Q + A EE+ + +Q+V V + PK + ++ +
Sbjct: 1083 NEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQ 1142

Query: 79 EQVYREAD 86
+ RE D
Sbjct: 1143 AEPAREND 1150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2947OMPADOMAIN701e-15 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 70.0 bits (171), Expect = 1e-15
Identities = 40/166 (24%), Positives = 70/166 (42%), Gaps = 37/166 (22%)

Query: 164 GEPPSAPKIEPEPEPTQPEPPSIAELGLDDLGDGVDVIVNEKSISFRISNELLFPSGQAV 223
G+ +AP + P P P P + F + +++LF +A
Sbjct: 192 GQGEAAPVVAPAPAP---APEVQTK-------------------HFTLKSDVLFNFNKAT 229

Query: 224 LSPAGLGLISRMAKVINR--SQGYPVSVEGHSDPVPIQTRQFPSNWELSAGRATSVLREL 281
L P G + ++ ++ + V V G++D I + + N LS RA SV+ L
Sbjct: 230 LKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDR--IGSDAY--NQGLSERRAQSVVDYL 285

Query: 282 VRDGVDPGRLRAVGYADTHPIASN--DTPQGRAA-------NRRVE 318
+ G+ ++ A G +++P+ N D + RAA +RRVE
Sbjct: 286 ISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVE 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2949HTHTETR568e-12 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.8 bits (134), Expect = 8e-12
Identities = 26/128 (20%), Positives = 46/128 (35%)

Query: 19 RDALVEATEAILAERGLEGFTLREAARRVGVSAAAPLHHFGSAAGLLTEVAILGFEALTR 78
R +++ + +++G+ +L E A+ GV+ A HF + L +E+ L +
Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72

Query: 79 HLREGARSGGNDPGARLRAQGMGYVRFALAHPARFQLMFRKDRLTDDARLAAASQAAFAE 138
E DP + LR + + + R LM + A Q A
Sbjct: 73 LELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRN 132

Query: 139 LEQAIRDY 146
L D
Sbjct: 133 LCLESYDR 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet2952PF05272300.032 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.032
Identities = 10/20 (50%), Positives = 12/20 (60%)

Query: 369 FVGPSGSGKSTLVKLLLGLY 388
G G GKSTL+ L+GL
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


97Bpet3011Bpet3021N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3011-127-6.876461putative tautomerase
Bpet3012-118-5.354754putative gluconate 5-dehydrogenase
Bpet3013013-4.173094ISRSO8-transposase orfA protein
Bpet3014110-3.707108transposase
Bpet301509-3.172711transposase
Bpet3016011-3.107417hypothetical protein
Bpet3017018-1.008013dihydrolipoamide dehydrogenase
Bpet3018-115-0.498900dihydrolipoamide acetyltransferase
Bpet3019-315-0.727070pyruvate dehydrogenase subunit E1
Bpet3020-314-0.172445two-component sensor kinase
Bpet3021-2130.802242two-component response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3011PF03944270.008 delta endotoxin
		>PF03944#delta endotoxin

Length = 633

Score = 26.6 bits (58), Expect = 0.008
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 2/46 (4%)

Query: 29 IKGVSDLLFEVMGKPRNSTFVVIEEV--DMDSWGVGGVTVAEYRKH 72
++G LL + + N I +V + D WG+ T+ YR +
Sbjct: 166 MQGYQLLLLPLFAQAANLHLSFIRDVILNADEWGISAATLRTYRDY 211


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3012DHBDHDRGNASE811e-20 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 81.3 bits (200), Expect = 1e-20
Identities = 60/254 (23%), Positives = 103/254 (40%), Gaps = 26/254 (10%)

Query: 6 KVAIVTGASQGLGAGIVESYRKRGFAVIANSRN----LKPSSDADVVA-----VPGDIGN 56
K+A +TGA+QG+G + + +G + A N K S A P D+ +
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 57 RDVAKQLVETAISRYGRVDTLINNAGIFIAKPFTQYTVEDMDRVFRTNLHGFFHVTQFAL 116
++ G +D L+N AG+ + E+ + F N G F+ ++
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128

Query: 117 EQMLKQERGHIVQITTTLVRQAIAGLDVGLTMLTKGGLEAVTRGLAIEYAKQGIRVNAVA 176
+ M+ + G IV + + + +K T+ L +E A+ IR N V+
Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSM--AAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 177 PGIINTPMH-------DPQAHDFLGGMH------PMGRMGEIADIAKAVMYL--EEADFV 221
PG T M + G + P+ ++ + +DIA AV++L +A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 222 TGETLNVDGGQQAG 235
T L VDGG G
Sbjct: 247 TMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3013HTHFIS260.014 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 25.9 bits (57), Expect = 0.014
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3014HTHFIS260.020 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.020
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 2/36 (5%)

Query: 10 QEFMLEAVRMVRGGQSMAAVAKILGISPKTLHNWVK 45
+L A+ RG Q AA +LG++ TL ++
Sbjct: 438 YPLILAALTATRGNQIKAA--DLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3017RTXTOXIND364e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.0 bits (83), Expect = 4e-04
Identities = 21/97 (21%), Positives = 39/97 (40%), Gaps = 6/97 (6%)

Query: 39 TVESDKASMEIPASSGGVVKSVKVKVGDKVAEGKVILQVEAGETK------EAKPAPAAS 92
+ S EI +VK + VK G+ V +G V+L++ A + ++ A
Sbjct: 89 KLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARL 148

Query: 93 QVSDSQRVSDTVTKEKAPQKGQPVNAAAQYSGSADVE 129
+ + Q +S ++ K P+ P Q +V
Sbjct: 149 EQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVL 185


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3018RTXTOXIND363e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.3 bits (84), Expect = 3e-04
Identities = 23/106 (21%), Positives = 41/106 (38%), Gaps = 8/106 (7%)

Query: 168 TVESDKASMEIPASAGGVVKEVKVKVGDKVAKGTAIAVVEGQGGAQAEPQKAQAP---AQ 224
+ S EI +VKE+ VK G+ V KG + + GA+A+ K Q+ A+
Sbjct: 89 KLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLT-ALGAEADTLKTQSSLLQAR 147

Query: 225 AQEQQPSGAASASAAAPAPA----AKPAPAAALEDPGLKPGQLPHA 266
++ + + + P +P E+ L+ L
Sbjct: 148 LEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKE 193



Score = 33.6 bits (77), Expect = 0.002
Identities = 19/94 (20%), Positives = 35/94 (37%), Gaps = 3/94 (3%)

Query: 39 TVESDKASMEIPASSGGVVKSVKVKVGDKVAEGKVILEVEAGEA---AGADQAPASPDKA 95
+ S EI +VK + VK G+ V +G V+L++ A A Q+ +
Sbjct: 89 KLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARL 148

Query: 96 DAAAKQPSSGDAPKTQGQTVEAPDVKPAADAGKG 129
+ Q S + ++ PD + +
Sbjct: 149 EQTRYQILSRSIELNKLPELKLPDEPYFQNVSEE 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3021HTHFIS1212e-34 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 121 bits (304), Expect = 2e-34
Identities = 39/152 (25%), Positives = 70/152 (46%)

Query: 6 QSSTVFIVDDDEAVRDSLRWLLEANGYRVRAFASGETFLEEYDPSQVGVLIADVRMPGMS 65
+T+ + DDD A+R L L GY VR ++ T +++ DV MP +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 66 GLELQEQILARNAPLPIVFITGHGDVPMAVSTMKKGAVDFLEKPFNESDLREIVARMLEQ 125
+L +I LP++ ++ A+ +KGA D+L KPF+ ++L I+ R L +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 126 ATQRVSQFQAQKDHEAMLARLTAREQQVLERI 157
+R S+ + L +A Q++ +
Sbjct: 122 PKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVL 153


98Bpet3129Bpet3137N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet31294190.134748hypothetical protein
Bpet31305190.028173GTP-binding elongation factor
Bpet31315181.533967tRNA pseudouridine synthase B
Bpet31326181.466085ribosome-binding factor A
Bpet31335161.417358translation initiation factor IF-2
Bpet31342141.303790transcription elongation factor NusA
Bpet3135-1132.531401hypothetical protein
Bpet3136-2132.870042pseudouridine synthase
Bpet3137-2151.732265transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3129MICOLLPTASE300.003 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 29.7 bits (66), Expect = 0.003
Identities = 11/49 (22%), Positives = 15/49 (30%), Gaps = 2/49 (4%)

Query: 83 ARFIRETYRAFEDI--LGELHPCCYVHVIDARAAAYGYGGATQEYRHQH 129
F E I L EL + H + R G G + Y+
Sbjct: 480 GTFFTYERTPEESIYTLEELFRHEFTHYLQGRYVVPGMWGQGEFYQEGV 528


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3130TCRTETOQM1634e-45 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 163 bits (414), Expect = 4e-45
Identities = 95/435 (21%), Positives = 166/435 (38%), Gaps = 62/435 (14%)

Query: 5 LRNVAIIAHVDHGKTTLVDQLLRQSGTFRENQAVSE--RVMDSNDLEKERGITILAKNCA 62
+ N+ ++AHVD GKTTL + LL SG E +V + D+ LE++RGITI +
Sbjct: 3 IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS 62

Query: 63 VEYQGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAVEGPMPQTIFVTRKALALGLK 122
+++ T +NI+DTPGH DF EV R LS++DG +LL+ A +G QT + +G+
Sbjct: 63 FQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP 122

Query: 123 PIVVVNKIDRPGAR-------------PDYVINATFDLFDKLGATEEQL----------- 158
I +NKID+ G + VI +L+ + T
Sbjct: 123 TIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGN 182

Query: 159 -DFPVVYASG--LSG---YAGLTAEVREGDMRPLF--------------EAILQHVPQRE 198
D Y SG L + + P++ E I
Sbjct: 183 DDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSST 242

Query: 199 DDPNGPLQMQIISLDYNSYVGKIGVGRINRGRIRPGMDVVYQFGPEGASGKGRINQVLKF 258
L ++ ++Y+ ++ R+ G + D V E K +I ++
Sbjct: 243 HRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLR-DSVRISEKE----KIKITEMYTS 297

Query: 259 KGLEREVVSEAEAGDIVLINGIEEIGIGCTVMDPAQPEAFPMLRIDEPTLTMNFMVNTSP 318
E + +A +G+IV++ E + + + D + P L +
Sbjct: 298 INGELCKIDKAYSGEIVILQN-EFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQ 356

Query: 319 LAGREGKFVTSRQLRDRLDRELKSNVALRVRDTGDDTVFEVSGRGELHLTILLETMRRE- 377
L D L S+ LR +S G++ + + ++ +
Sbjct: 357 QREM---------LLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKY 407

Query: 378 GYELAVSRPRVVFKD 392
E+ + P V++ +
Sbjct: 408 HVEIEIKEPTVIYME 422



Score = 31.4 bits (71), Expect = 0.014
Identities = 16/88 (18%), Positives = 29/88 (32%), Gaps = 2/88 (2%)

Query: 399 EPFEALTIDVEDAHQGGVMEELGRRKGDLQDMQPDGRGRTRLEYLIPARGLIGFQNEFLT 458
EP+ + I + + + ++ D Q L IPAR + ++++
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQL-KNNEVILSGEIPARCIQEYRSDLTF 595

Query: 459 LTRGTGLMSHIFHEYAP-VREGSIGERR 485
T G + Y E RR
Sbjct: 596 FTNGRSVCLTELKGYHVTTGEPVCQPRR 623


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3133TCRTETOQM832e-18 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 83.4 bits (206), Expect = 2e-18
Identities = 68/277 (24%), Positives = 98/277 (35%), Gaps = 76/277 (27%)

Query: 499 VMGHVDHGKTSLLDYI-----RRAKVASGEAG-------------GITQHIGAYHVETAR 540
V+ HVD GKT+L + + ++ S + G GIT G +
Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67

Query: 541 GVVTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTREAIHHAKAGGVPLVVAV 600
V +DTPGH F A R D IL+++A DGV QTR H + G+P + +
Sbjct: 68 TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFI 127

Query: 601 NKIDKPEANPERVKQ--------------------------------------------- 615
NKID+ + V Q
Sbjct: 128 NKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLE 187

Query: 616 ------ELVAEEVVPEEYGG--DVPFVPV---SAKTGAGIDDLLENVLLQAEILELTAPV 664
L A E+ EE + PV SAK GID+L+E + + T
Sbjct: 188 KYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVIT--NKFYSSTHRG 245

Query: 665 EAPAKGLVIEARLDKGRGPVATILVQSGTLKRGDVVL 701
++ G V + + R +A I + SG L D V
Sbjct: 246 QSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVR 282


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3136cloacin300.024 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 30.5 bits (68), Expect = 0.024
Identities = 26/101 (25%), Positives = 33/101 (32%)

Query: 404 GRRGKLQGGGPGSAGHVASSPSDPFGTGLMFAGGYANGHPLGKDAGRGKGGGKPGGKSGG 463
G L GG S G SS ++P+G G + G G G G GG G
Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81

Query: 464 KPGGFKAAGAKAGGAKPGGAKPAKNAKARRPKPGVAGGAAA 504
A + PG A + A +A AA
Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3137IGASERPTASE394e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 38.5 bits (89), Expect = 4e-05
Identities = 30/148 (20%), Positives = 41/148 (27%), Gaps = 15/148 (10%)

Query: 201 AAAEAGVSVEANAAAETEAAEEAAIQEAVVEEAVAAPDAAAVEGVAAEADAVAVAEDPAP 260
A EA +V+AN A + +E E E E + E P
Sbjct: 1068 VAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQ--EVPKV 1125

Query: 261 VAQTGDLPAEPDMPPPAPDHIPQPGAQPEVEPVAPEPEITPADP---QPEIEPPTPE--P 315
+Q P QP A+P E P + E P E
Sbjct: 1126 TSQVS--------PKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSS 1177

Query: 316 EVEPRLPDPDVEPPAPEVPPTPDEDQPA 343
VE + + V P+ PA
Sbjct: 1178 NVEQPVTESTTVNTGNSVVENPENTTPA 1205



Score = 37.0 bits (85), Expect = 1e-04
Identities = 27/163 (16%), Positives = 42/163 (25%), Gaps = 14/163 (8%)

Query: 202 AAEAGVSVEANAAAETEAAEEAAIQEAVVEEAVAAPDAAAVEGVAAE-------ADAVAV 254
E S +T +E A E + V V V ++ ++ V
Sbjct: 1082 TNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQP 1141

Query: 255 AEDPAPVAQTGDLPAEPDMPPPAPDHIPQPGAQPEVEPVAPEPEITPADPQPEI------ 308
+PA EP QP + P E T + +
Sbjct: 1142 QAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPEN 1201

Query: 309 -EPPTPEPEVEPRLPDPDVEPPAPEVPPTPDEDQPAKGGLSQA 350
P T +P V + V P +PA +
Sbjct: 1202 TTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDR 1244


99Bpet3195Bpet3202N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet31953140.955977sulfite reductase
Bpet31961130.512631hypothetical protein
Bpet31970120.588954outer membrane porin protein precursor
Bpet3198-1141.447387TonB-dependent outer membrane receptor
Bpet3199-1162.051463putative dicarboxylic acid hydrolase
Bpet3200-1161.505160putative transporter transmembrane protein
Bpet3201-1181.784334GntR family transcriptional regulator
Bpet32020151.076872MFS permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3195PF07520290.047 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 29.2 bits (65), Expect = 0.047
Identities = 20/96 (20%), Positives = 26/96 (27%), Gaps = 1/96 (1%)

Query: 240 PPGVRVALPMSDEHLGHTGPTTWSLEQARMPESAYAHAMGQPSQPIGLDAAVAAFDRLGL 299
+ P H + E R A + AA+ F +
Sbjct: 42 RSFRFIERPEGAAEGRHRTLYPLTGEAERDAPILAATTPEDDEYSVRPLAALEPFLEKWV 101

Query: 300 -APGYAINVPHGAAGVYTGSVYPSDLARQRVVHLDQ 334
P + GA G PS AR R V L Q
Sbjct: 102 PIPVLRLKNQRGAGGEELYDPGPSSWARLRTVELPQ 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3197ECOLNEIPORIN947e-24 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 94.1 bits (234), Expect = 7e-24
Identities = 91/372 (24%), Positives = 142/372 (38%), Gaps = 44/372 (11%)

Query: 1 MKKTLLAAALLAGFAGAAQAETSVTLYGVIDTGIGYNK-IKGDGYDGSRIGMINGI-QAG 58
MKK+L+A LA AA A VTLYG I G+ ++ + +G + + GI G
Sbjct: 1 MKKSLIAL-TLAALPVAAMA--DVTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLG 57

Query: 59 SRWGLRGSEDLGDGLRAVFQLESGFDSGNGNRAQGGRLFGRQATIGLANDSWGILEFGRQ 118
S+ G +G EDLG+GL+A++Q+E + G R Q+ IGL +G L GR
Sbjct: 58 SKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNR----QSFIGLKGG-FGKLRVGRL 112

Query: 119 TNMASKYLADIDPFYTSYTQANLGLGASSANTSRWDNMVMYRTPSVNGFELGVGYSFNVD 178
+ K DI+P+ + LG A V Y +P G V Y+ N +
Sbjct: 113 NS-VLKDTGDINPWDSKSDY--LG-VNKIAEPEARLISVRYDSPEFAGLSGSVQYALNDN 168

Query: 179 DGNADQTGFRTADNTRGITAGLRYLNGPLNITLTYDQLNGSNSSSQIDHDATPRQYAAGV 238
G N+ AG Y NG + ++ + + +
Sbjct: 169 AGR---------HNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIE-KYQIHRLVS 218

Query: 239 SYDFEVVKLAAAYARTTDGWFTGQDLPSGTPYSNEFGSNRFVDGFKANAYMVGGTVP-IG 297
YD + + A+ + D + N + AY G P +
Sbjct: 219 GYDNDAL-YASVAVQQQDA----------KLVEENYSHNSQTEVAATLAYRFGNVTPRVS 267

Query: 298 GASSVFASWQRVDPSNDRLTGGDSTMNVWSLGYTYDLSKRTNLYAYGSYGKDYAFIDGLK 357
A S+ ++ + +G YD SKRT+ + ++
Sbjct: 268 YAHGFKGSFDAT--------NYNNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFV 319

Query: 358 STAAGVGIRHQF 369
STA GVG+RH+F
Sbjct: 320 STAGGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3200TCRTETB491e-08 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 49.5 bits (118), Expect = 1e-08
Identities = 77/418 (18%), Positives = 138/418 (33%), Gaps = 67/418 (16%)

Query: 27 LDYMVFTFVISTLVTLWGIDRGQAGMLGTVTLLFSAIGGWGAGILADRYGRVRILQITIL 86
L+ MV + + + + T +L +IG G L+D+ G R+L I+
Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87

Query: 87 WFSVCTVGIGFTQNFEQIFIL-RALQGLGFGGEWAVGSVLMGEIIKSQHRARAVGTVQSG 145
+V +F + I+ R +QG G A+ V++ I ++R +A G + S
Sbjct: 88 INCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSI 147

Query: 146 WAVGWGVAALLYTIAFSVLPEQWAWRSLFWVGVLPALLVLYIRKHVPEPE---------- 195
A+G GV + + + W L + ++ + V ++ K + +
Sbjct: 148 VAMGEGVGPAIGGM----IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKG 203

Query: 196 -----------------------------VFSHARKQPTADRPKVSPWLIFSPALLKTTL 226
+ P V P L + + L
Sbjct: 204 IILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVL 263

Query: 227 LSALLCTGIQGGYYAVTTWLPTYLKVERHLSVLNTGGYLL--VIILGSFCGYIAGAHMAD 284
+ I G + +P +K LS G ++ + GYI G + D
Sbjct: 264 CGGI----IFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGI-LVD 318

Query: 285 RLGRRINFVIYSVLSGVCVYVYTQVPLTDEQMLFLGFPLGFAASGIFGGLGAYLTELFPS 344
R G I V + + T + + + GGL T +
Sbjct: 319 RRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVF------VLGGLSFTKTVISTI 372

Query: 345 EIRANGQGFAYNFGRGIGALFPSLVGYLSNSMGLAMAIGIFAGGAYTVVLIATLLLPE 402
+ Q A G G+ L + +LS G+A+ GG ++ L+ LLP
Sbjct: 373 VSSSLKQQEA---GAGMSLL--NFTSFLSEGTGIAI-----VGGLLSIPLLDQRLLPM 420



Score = 32.9 bits (75), Expect = 0.002
Identities = 28/155 (18%), Positives = 56/155 (36%), Gaps = 11/155 (7%)

Query: 59 LFSAIGGWGAGILADRYGRVRILQITILWFSVCTVGIGFTQNFEQIFILRALQGLGFGGE 118
+ I G+ GIL DR G + +L I + + SV + F F+ + + GG
Sbjct: 304 MSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFV-LGGL 362

Query: 119 WAVGSVLMGEIIKSQHRARAVG-------TVQSGWAVGWGVAALLYTIAF---SVLPEQW 168
+V+ + S + A T G + L +I +LP +
Sbjct: 363 SFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEV 422

Query: 169 AWRSLFWVGVLPALLVLYIRKHVPEPEVFSHARKQ 203
+ + +L + + + V+ H+++
Sbjct: 423 DQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRD 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3202TCRTETA354e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 35.2 bits (81), Expect = 4e-04
Identities = 47/177 (26%), Positives = 68/177 (38%), Gaps = 4/177 (2%)

Query: 57 IAVHNLVWGAAQPFAGAAADRYGSAPVVAFGAAAFAAGLALATAAQSPVLLVIGMGVLVG 116
+A++ L+ A P GA +DR+G PV+ A A A+ A +P L V+ +G +V
Sbjct: 49 LALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI--MATAPFLWVLYIGRIVA 106

Query: 117 IGISCTSFGVVLAAVGRAATPQRRSMALGLASAGGSVGQVALVPFAQVLRESAGVSASLL 176
GI+ + V A + R+ G SA G VA P L A
Sbjct: 107 -GITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVA-GPVLGGLMGGFSPHAPFF 164

Query: 177 GLAALMLLVAPLGMLLDRPAAQGGAPTAQEPALSLKQAVLHACRHRGYCLLTLGFFT 233
AAL L G L + +G + AL+ + A L FF
Sbjct: 165 AAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFI 221


100Bpet3320Bpet3325N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3320-3111.237584penicillin-binding protein precursor
Bpet3321-3151.978243putative chloride channel protein
Bpet3322-2242.192414histone-like protein
Bpet3323-3232.188607major facilitator superfamily permease
Bpet3324-3201.728794leucyl-tRNA synthetase
Bpet33250142.880910putative lipoprotein precursor
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3320BLACTAMASEA362e-04 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 35.9 bits (83), Expect = 2e-04
Identities = 25/111 (22%), Positives = 47/111 (42%), Gaps = 6/111 (5%)

Query: 22 LLDATSGQEMASFNADTRVEPASLTKVMTAYLVFQALRDGRLSTQQLVTVSTRAWKV-AP 80
+D SG+ + ++ AD R S KV+ V + G ++ + + +P
Sbjct: 44 EMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSP 103

Query: 81 GSSKMFLEPGKRVSVNDLLFGLLVQSGNDAAIVLAEAVSG--SVEAFVQRM 129
S K ++V +L + S N AA +L V G + AF++++
Sbjct: 104 VSEKHL---ADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQI 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3322DNABINDINGHU365e-06 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 36.2 bits (84), Expect = 5e-06
Identities = 30/97 (30%), Positives = 42/97 (43%), Gaps = 8/97 (8%)

Query: 46 NKSQLIAHLVEQTGVEAKSVKAVLAGLEGAVLGSVDKKGAGEFSLPGLFKVTVQKVPAKA 105
NK LIA + E T + K A + + AV + K + G F+V +A
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVR-----ERA 57

Query: 106 KRFGKDPFTGEERWFPAKPASVKVKVRPLKKLKDAAQ 142
R G++P TGEE AS + K LKDA +
Sbjct: 58 ARKGRNPQTGEE---IKIKASKVPAFKAGKALKDAVK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3323TCRTETA340.001 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 34.0 bits (78), Expect = 0.001
Identities = 27/146 (18%), Positives = 49/146 (33%), Gaps = 6/146 (4%)

Query: 38 PLLHTIGQQFGLSETAAGGIVTTAQLSYAAGLLLLVPLG----DMIERRALICAMTALAA 93
P+L + + S L YA P+ D RR ++ A AA
Sbjct: 26 PVLPGLLRDLVHSNDVTAHYGILLAL-YALMQFACAPVLGALSDRFGRRPVLLVSLAGAA 84

Query: 94 AGLLLSAFSTSAAMLLAGTAITGFLSVVAQVLVPFAATLASPQQRGKAVGTVMSGLLLGI 153
+ A + +L G + G V + A + +R + G + + G+
Sbjct: 85 VDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGM 144

Query: 154 LLARTFAGMLAGLGSWRIVYWVAALL 179
+ G++ G ++ AA L
Sbjct: 145 VAGPVLGGLMGGFSP-HAPFFAAAAL 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3325PF04335270.042 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 27.5 bits (61), Expect = 0.042
Identities = 14/58 (24%), Positives = 20/58 (34%), Gaps = 1/58 (1%)

Query: 14 RWLLRAASLAAVMLLAACGFALRGVTPLPFDTLYVGIADNTRFGADIRRALRAASPNT 71
+ A +A + A A+ +TPL YV D A I L + T
Sbjct: 33 KLAWVVAGVAGALATAGV-VAVAALTPLKTVEPYVITVDRNTGEASIAAKLHGDATIT 89


101Bpet3495Bpet3503N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet3495-39-0.490763putative amino acid ABC transporter ATP-binding
Bpet3496-38-0.305478hypothetical protein
Bpet3497-28-0.632958putative phospholipase
Bpet3498-29-0.194912putative ABC transporter substrate binding
Bpet3499-290.548727hypothetical protein
Bpet3500-1100.620366ArsR family transcriptional regulator
Bpet35010111.581929hypothetical protein
Bpet35021121.473094hypothetical protein
Bpet35031121.896170propionate catabolism operon regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3495PF05272320.002 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 32.4 bits (73), Expect = 0.002
Identities = 12/23 (52%), Positives = 16/23 (69%)

Query: 38 VLVVVGPSGSGKSTLLRTLNGLE 60
+V+ G G GKSTL+ TL GL+
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3497PHPHLIPASEA1903e-22 Bacterial phospholipase A1 protein signature.
		>PHPHLIPASEA1#Bacterial phospholipase A1 protein signature.

Length = 289

Score = 89.6 bits (222), Expect = 3e-22
Identities = 57/223 (25%), Positives = 90/223 (40%), Gaps = 32/223 (14%)

Query: 236 TTARFQISAKYRLFNPPGGRKPTFGENFYLGYTQTSLWDLESD--SMPFIDTTFNPSIF- 292
+FQ+S + L+ G YTQ S W L + S PF +T + P +F
Sbjct: 85 DEVKFQLSLAFPLWRGILGPN----SVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFL 140

Query: 293 -WLSD---NLWTSSSQNWRLGLNTGIEHMSNGKSGDDSRSLNDAYIQPAINYRFDSGSTL 348
+ +D WT + G H SNG+S SRS N Y +
Sbjct: 141 GFATDYRFAGWTLRD------VEMGYNHDSNGRSDPTSRSWNRLYTRLMAEN-----GNW 189

Query: 349 TFAPKIRTYFAKESQNPDYADYAGYVDWNLRWAQDDGAVVSAMYRQGASR-HRTTQLDFA 407
K NPD Y GY + + D AV+SA + + + +L +
Sbjct: 190 LVEVKPWYVVGNTDDNPDITKYMGYYQLKIGYHLGD-AVLSAKGQYNWNTGYGGAELGLS 248

Query: 408 WPLRRTWLDMNGYLHLQYFNGYGETLLGYNQRNESQFRIGLSL 450
+P+ + + L+ Q ++GYGE+L+ YN Q R+G+ +
Sbjct: 249 YPITK-----HVRLYTQVYSGYGESLIDYNFN---QTRVGVGV 283


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3499TYPE3IMRPROT310.008 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 30.9 bits (70), Expect = 0.008
Identities = 16/83 (19%), Positives = 27/83 (32%), Gaps = 12/83 (14%)

Query: 157 HSTGSIGVFGAAVAIGKLLGLEAAQMVWAIGLAATQSAGLR---EMFGSMAKSFHPGRSA 213
S ++ + + IG LG A+ ++AG +M S A P
Sbjct: 66 FSFFALWLAVQQILIGIALGFTMQFAFAAV-----RTAGEIIGLQMGLSFATFVDPASHL 120

Query: 214 QSGYVAALLAQRG----FTAGGH 232
+A ++ T GH
Sbjct: 121 NMPVLARIMDMLALLLFLTFNGH 143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3502TCRTETB402e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 39.9 bits (93), Expect = 2e-05
Identities = 34/157 (21%), Positives = 64/157 (40%), Gaps = 4/157 (2%)

Query: 39 MAQDLGFSRSLLSGIVAIGMLCYGLGMPVAGMLVARRGTRFVLLLGTAI-VVGSIIWTVN 97
+A D + + + ML + +G V G L + G + +LL G I GS+I V
Sbjct: 40 IANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVG 99

Query: 98 ARGPVSFLLAFGVLMSVGLAFTSPVALTPVLTRWFTRRRGMALFFLSTGSMAGIAVMTPA 157
S L+ + G A + + V RG A + + +A + PA
Sbjct: 100 -HSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGS-IVAMGEGVGPA 157

Query: 158 LTYAVESTTWQTTLLGFAVVFTVLTVPMAIFVMRDQA 194
+ + + LL ++ T++TVP + +++ +
Sbjct: 158 IGGMIAHYIHWSYLLLIPMI-TIITVPFLMKLLKKEV 193



Score = 28.7 bits (64), Expect = 0.049
Identities = 28/141 (19%), Positives = 52/141 (36%), Gaps = 8/141 (5%)

Query: 20 LLTLLTVGMRM--GVGPFFLPMAQDLGFSRSLL-----SGIVAIGMLCYGLGMPVAGMLV 72
+ + G + V F + + L S I+ G + + + G+LV
Sbjct: 258 FMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILV 317

Query: 73 ARRGTRFVLLLGTAIVVGSIIWTVNARGPVSFLLAFGVLMSVG-LAFTSPVALTPVLTRW 131
RRG +VL +G + S + S+ + ++ +G L+FT V T V +
Sbjct: 318 DRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSL 377

Query: 132 FTRRRGMALFFLSTGSMAGIA 152
+ G + L+ S
Sbjct: 378 KQQEAGAGMSLLNFTSFLSEG 398


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3503HTHFIS346e-115 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 346 bits (889), Expect = e-115
Identities = 134/392 (34%), Positives = 197/392 (50%), Gaps = 49/392 (12%)

Query: 274 LRLGGRDWLANRMPIRERGAVVGAALTLYDAGRIHEADTSLRVQQRRRQNTAKYQFAELI 333
G D+L + E ++G AL + ++ + L+
Sbjct: 94 SEKGAYDYLPKPFDLTELIGIIGRAL-------------AEPKRRPSKLEDDSQDGMPLV 140

Query: 334 GRSPPFLRAVRTARRYAQTDLTVLIAGESGVGKELFAQAIHNESRRADRPFVAVNCASFP 393
GRS R R QTDLT++I GESG GKEL A+A+H+ +R + PFVA+N A+ P
Sbjct: 141 GRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIP 200

Query: 394 ESLLESELFGYEEGAFTGSRRGGKRGLFEAAHTGTLFLDEIGDMPLPLQSRLLRVLQERE 453
L+ESELFG+E+GAFTG++ G FE A GTLFLDEIGDMP+ Q+RLLRVLQ+ E
Sbjct: 201 RDLIESELFGHEKGAFTGAQTRST-GRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGE 259

Query: 454 VTRLGATAAIPVDVRIIAATHQPLSDMVAQRRFRQDLYYRINTLRLEVPPLRERPDDIQP 513
T +G I DVRI+AAT++ L + Q FR+DLYYR+N + L +PPLR+R +DI
Sbjct: 260 YTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPD 319

Query: 514 LLETLIDRCLRRLGAPLQAAGLVAPWLPRLRRYAWPGNVRELENISERMAVF-------- 565
L+ + + + G L ++ + WPGNVRELEN+ R+
Sbjct: 320 LVRHFVQQ-AEKEGLD--VKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITR 376

Query: 566 -LMQYPHAADVDH----------------DALRHDCPELFEAGAAVAADEG-------RS 601
+++ +++ A+ + + F + G
Sbjct: 377 EIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEM 436

Query: 602 QRDRALRALEACGGNRQEAARRLGISRSTLWR 633
+ L AL A GN+ +AA LG++R+TL +
Sbjct: 437 EYPLILAALTATRGNQIKAADLLGLNRNTLRK 468


102Bpet3581Bpet3587N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet35811132.408111TetR family transcriptional regulator
Bpet35822153.536637outer membrane efflux protein
Bpet35832153.746264MFS family transporter
Bpet35840123.682907secretion protein
Bpet35850123.787538LysR family transcriptional regulator
Bpet35861144.396328hypothetical protein
Bpet35870144.007494MFS permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3581HTHTETR661e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 66.2 bits (161), Expect = 1e-15
Identities = 37/186 (19%), Positives = 64/186 (34%), Gaps = 17/186 (9%)

Query: 26 AVLTAAREVFLTHGFSAATTDMIQRAAGVSKATVYAYYPTKQALFEAVIEGKCAEHM--A 83
+L A +F G S+ + I +AAGV++ +Y ++ K LF + E ++
Sbjct: 15 HILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE-LSESNIGEL 73

Query: 84 TLRSLRSVPGAIHAVLSELANAYLEFGVAPEGLALFRV-------SAAEAPRFPELARAF 136
L PG +VL E+ LE V E L E + R
Sbjct: 74 ELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNL 133

Query: 137 FENGPEAYCGIVAEHVERGVRNQELHLGDVSPQEAARLFFSLVRGQAQLEGVLLPDRRPS 196
+ + +E L D+ + AA + + G +E L +
Sbjct: 134 CLESYDRIEQTLKHCIEAK----MLPA-DLMTRRAAIIMRGYISG--LMENWLFAPQSFD 186

Query: 197 EAQKKR 202
++ R
Sbjct: 187 LKKEAR 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3582RTXTOXIND310.017 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.017
Identities = 27/155 (17%), Positives = 52/155 (33%), Gaps = 8/155 (5%)

Query: 70 LDALVARAWDGNLDLQAAAARVEQSRARAGVALAQL--FPRVDLDASLTRGAISENGPMA 127
L AL A A AR+EQ+R + +L P + L +SE +
Sbjct: 127 LTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLR 186

Query: 128 ALGAPTSTTDLWRAGFQADWEIDLWGRLRRQREGAVATLQATLYEQRSAQVALSAE---I 184
W+ + E++L + R +R +A + R + L +
Sbjct: 187 LTSLIKEQFSTWQNQ-KYQKELNL-DKKRAERLTVLARINRYENLSRVEKSRLDDFSSLL 244

Query: 185 ARQYVA-LRGVQTRLDIARRNQEIAAHLLRLTETR 218
+Q +A ++ E+ + +L +
Sbjct: 245 HKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIE 279


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3583TCRTETB381e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 37.6 bits (87), Expect = 1e-04
Identities = 68/411 (16%), Positives = 144/411 (35%), Gaps = 40/411 (9%)

Query: 37 INNRVGALALADIRGAGGFGLDDASWITTAYTAGELIAMPLAPWFAVTLSLRRFHL--QM 94
+N V ++L DI +W+ TA+ I + + L ++R L +
Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87

Query: 95 LAAGAAIAGVLPFVHDLRLLLLLRGLQGVASGALIPLLMMAALRFFPPSIRLFALALYSM 154
+ ++ G + LL++ R +QG + A L+M+ R+ P R A L
Sbjct: 88 INCFGSVIGFVGHSF-FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGS 146

Query: 155 TATFAPNVSTWLTGLWTDQLVDLRMVYWQIVPINLLAGLLVAWGIPQDRPLPERFRHANW 214
V + G+ + Y ++P+ + + + + R
Sbjct: 147 IVAMGEGVGPAIGGMIAHY---IHWSYLLLIPMITIITVPFLMKLL-----KKEVRIKGH 198

Query: 215 LGMAFGGAGLLLLAIGIEQGNRLEWFTSPLVCTSLSAGSLLL---AFYLFTEWHHPT--P 269
+ G++L+++GI L TS S L++ +F +F + P
Sbjct: 199 FDI----KGIILMSVGI--------VFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDP 246

Query: 270 FIKLQLLKRRNLWLGFSLFLCLLVIFLSGSLLPATLLGHAWHYRALQSAPIGLMIGLPQL 329
F+ L K +G + + ++ L +A IG +I P
Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQ---LSTAEIGSVIIFP-G 302

Query: 330 VVAPAVAMLLYQKWVDARA---VMAAGMAITAAACLLGAQVTNQWMWPEFALAQGLQAVG 386
++ + + VD R V+ G+ + + L + + W + + V
Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSW---FMTIIIVFVL 359

Query: 387 QPMAIVAMLF--LATSMVAPQEGPYVSGIVNLLRALGAPLGSALISRVIEL 435
++ + + +S + QE ++N L G A++ ++ +
Sbjct: 360 GGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSI 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3584RTXTOXIND1061e-27 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 106 bits (266), Expect = 1e-27
Identities = 50/415 (12%), Positives = 118/415 (28%), Gaps = 84/415 (20%)

Query: 4 SKKTKLAGSATVMVAAVALAL-IFNRPESAAATQSTDDAYIRAEITSVAPEITGLVEAVL 62
S++ +L + +A L + + E A + P +V+ ++
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGK--LTHSGRSKEIKPIENSIVKEII 111

Query: 63 VEENQPVRAGQLLV---------------------------------------------- 76
V+E + VR G +L+
Sbjct: 112 VKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLP 171

Query: 77 ------QIDDREYVLAERNAAAALAHARAAADGIHAQIEVQQSVIRQAQSTIEADQATRE 130
+ + E + + + ++ +++ + I +
Sbjct: 172 DEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSR 231

Query: 131 LARLDYSRYKSLAADGSGTVQARQQAKARL---------------QVEKAQQTKDQAILQ 175
+ + + SL + A + + + Q+E + +
Sbjct: 232 VEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQL 291

Query: 176 AQKGRQAALQADLLRAQAEIRQAEAALAQARLDLSRTRITAPIAGTIGHKRVR-VGNYAR 234
+ + + L + I LA+ + I AP++ + +V G
Sbjct: 292 VTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVT 351

Query: 235 TGDPLLTLVPLTDIY-IEANFRETQLARMRQGQPVRVTVDALPGRTF---TGTVQSLGPA 290
T + L+ +VP D + A + + + GQ + V+A P + G V+++
Sbjct: 352 TAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLD 411

Query: 291 SGVSYSVIAPHNATGNFTKIVQRLPVRIALDPQQDGADQLRVGMSVQPEVDVNAR 345
+ G ++ + + L GM+V E+ R
Sbjct: 412 A-------IEDQRLGLVFNVIISIEENCLS--TGNKNIPLSSGMAVTAEIKTGMR 457


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet3587TCRTETB290.028 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.5 bits (66), Expect = 0.028
Identities = 31/173 (17%), Positives = 62/173 (35%), Gaps = 3/173 (1%)

Query: 37 VMNLFAVQTVAPVIAASLGLGLDSVGVLAMLPQLGYALGLVLLVPLADRLENRRLIGATL 96
V+N + P IA S + L +++G + L+D+L +RL+ +
Sbjct: 27 VLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGI 86

Query: 97 AVCALCMLAAAFAPGGA--VFMAAVFAGGASTCAIQMLVPMAAFMAAPERRGATVGNVMS 154
+ + + MA G + +++ + A E RG G + S
Sbjct: 87 IINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGS 146

Query: 155 GLMVGVLLSRPLSNLVVDAWGWRALYLVFAGGMAATGVALLCLLPQRRPHDGP 207
+ +G + + ++ W L L+ + T L+ LL + G
Sbjct: 147 IVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITI-ITVPFLMKLLKKEVRIKGH 198


103Bpet4184Bpet4195N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4184321-3.264037HTH-type transcriptional regulator AcrR family
Bpet4185320-2.903321TetR family transcriptional regulator
Bpet4186220-3.284756hypothetical protein
Bpet4187219-3.504775outer membrane efflux protein
Bpet4188222-4.454231AcrB/AcrD/AcrF family protein
Bpet4189122-4.184700multidrug resistance protein
Bpet4190019-3.401925TetR family transcriptional regulator
Bpet4191016-2.409732TetR family transcriptional regulator
Bpet4192-115-1.476006hypothetical protein
Bpet4193-2140.092007hypothetical protein
Bpet4194-1130.329828hypothetical protein
Bpet4195-1190.0248152-hydroxymuconic semialdehyde hydrolase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4184HTHTETR952e-26 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 95.1 bits (236), Expect = 2e-26
Identities = 52/209 (24%), Positives = 82/209 (39%), Gaps = 5/209 (2%)

Query: 1 MAGQRKIDALETRERILDAAEWCFCAYGVSHASLEAIAEKASCTRGAIYWHFSGHADLIK 60
MA + K +A ETR+ ILD A F GVS SL IA+ A TRGAIYWHF +DL
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 GIMERGLPPYTKRLEALSYA-PSPLIQKIRECLQECFAAIDGDQHVRNALTILLLRNDFL 119
I E + P + +RE L + ++ R + I+ + +F+
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 120 GLREPFLDSRYQESIEVTAPLALAFRRAISNGEMSSALDPEICAEMINSTMLGILRRSLL 179
G ++ +E + + I + + L A ++ + G++ L
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 180 R-NSCVLAGTGADVLEMAFALIAGISHRP 207
S L D + L+ P
Sbjct: 181 APQSFDLKKEARDYVA---ILLEMYLLCP 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4185HTHTETR598e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.9 bits (142), Expect = 8e-13
Identities = 36/165 (21%), Positives = 55/165 (33%), Gaps = 3/165 (1%)

Query: 14 TPEEILDAAEWCFLHLGVAGTSTALIAARTRCARSLVSAHFPSPRSILQEVLYRGRLPLI 73
T + ILD A F GV+ TS IA R + HF + E+ +
Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71

Query: 74 GHLRRVKATQT-QLIPALRSALQLCLNDILHNERVRATQEILLFHCDLRHLPKDVLEQQI 132
+A + LR L L + ER R EI +FH V++Q
Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEI-IFHKCEFVGEMAVVQQAQ 130

Query: 133 KESAEAM-ALLRSIAVDAKRAGELRENICPESWASILGQLLSGAV 176
+ + A L ++ A I+ +SG +
Sbjct: 131 RNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLM 175


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4188ACRIFLAVINRP11970.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1197 bits (3098), Expect = 0.0
Identities = 605/1032 (58%), Positives = 789/1032 (76%), Gaps = 6/1032 (0%)

Query: 1 MSRFFIDRPIFAWVVAIVIMLAGALSILSLPVNQYPNIAPPAIGIIANYPGASAQTVQDT 60
M+ FFI RPIFAWV+AI++M+AGAL+IL LPV QYP IAPPA+ + ANYPGA AQTVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQQLNGLDGLRYIRSESNADGSVTIVVTFEQGVNPDIAQVQVQNKLSLATPMLPQ 120
VTQVIEQ +NG+D L Y+ S S++ GSVTI +TF+ G +PDIAQVQVQNKL LATP+LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 AVQQLGLRVVKYQVNFMLVAALISEDGRLDNYALADQIVSQLQDPLTRTAGVGDFFVMGS 180
VQQ G+ V K ++++VA +S++ ++D + S ++D L+R GVGD + G+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QNAMRVWLDPLKLNNYALTPGDVIAAIEEQNVQVSSGQLGGRPTAGKVELNATVIGKTLL 240
Q AMR+WLD LN Y LTP DVI ++ QN Q+++GQLGG P +LNA++I +T
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 QTPEQFGAMLLKVNTDGSQVRLRDVADVALGADNFSITTRYNGKPSAGIALRLASGGNTL 300
+ PE+FG + L+VN+DGS VRL+DVA V LG +N+++ R NGKP+AG+ ++LA+G N L
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 EAVKAVQETLSRLEPTLPPGVKVVYPYNTAPVVSESINGVVHTLLEAIVLVFVIMYLFLQ 360
+ KA++ L+ L+P P G+KV+YPY+T P V SI+ VV TL EAI+LVF++MYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NWRATLIPTLAVPVVLLGTFGVMAAVGFTINTLTMFGLVLAIGLLVDDAIVVVENVERLM 420
N RATLIPT+AVPVVLLGTF ++AA G++INTLTMFG+VLAIGLLVDDAIVVVENVER+M
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 VEEGLSPLEATRKSMEQISGALVGIGLVLSAVFIPMAFFGGSTGVIYRQFSLTIVTAMTL 480
+E+ L P EAT KSM QI GALVGI +VLSAVFIPMAFFGGSTG IYRQFS+TIV+AM L
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVFVALIFTPALCATMLKPVHGHH--EKKGFFGWFNRMFERNAQRYESGVTRVVAGRGRY 538
SV VALI TPALCAT+LKPV H K GFFGWFN F+ + Y + V +++ GRY
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 539 MLAFALIVGALAVLFPMMPTSFLPDEDQGTMVVQVELPTNSTADQTDQLLNELSTYLLEE 598
+L +ALIV + VLF +P+SFLP+EDQG + ++LP +T ++T ++L++++ Y L+
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 599 EGDVVDSVFAVNGFSFAGRGQNSGLAFVQLKPWEERKR---SVFDLQASAMQRFSEVKAG 655
E V+SVF VNGFSF+G+ QN+G+AFV LKPWEER S + A +++ G
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 656 TALAFAPPAIQELGNATGFNLFLQDYRGEGHEQLMQVRGQFLAEASKHPA-LTLVRPNGK 714
+ F PAI ELG ATGF+ L D G GH+ L Q R Q L A++HPA L VRPNG
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 715 PDEPQYQVIIDDEKARALGVTLAEVNRTMSTAWGSSYVNDFIDRGRVKRVYVQGIPQARI 774
D Q+++ +D EKA+ALGV+L+++N+T+STA G +YVNDFIDRGRVK++YVQ + R+
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 775 TPEDFNKWYVRNKNGQMVSFASFATGKWVYGSPKLERYNGVPAIEILGEPAPGYSSGDAM 834
PED +K YVR+ NG+MV F++F T WVYGSP+LERYNG+P++EI GE APG SSGDAM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 835 RAVEEIAAKLPSGVSLAWTGLSYEERLSGSQAPALYALSIVAVFLCLAALYESWSIPFSV 894
+E +A+KLP+G+ WTG+SY+ERLSG+QAPAL A+S V VFLCLAALYESWSIP SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 895 LLVVPLGVIGTVAATLMRGLENDAFFQIGLLTTVGLCAKNAILIVEFAKDLHEKGGRTLV 954
+LVVPLG++G + A + +ND +F +GLLTT+GL AKNAILIVEFAKDL EK G+ +V
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 955 QAAIEASRLRLRPIIMTSLAFTMGVIPLAISSGASSGSKHAIGTGVIGGMVTATFLAIFF 1014
+A + A R+RLRPI+MTSLAF +GV+PLAIS+GA SG+++A+G GV+GGMV+AT LAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1015 IPLFYVVVSSLF 1026
+P+F+VV+ F
Sbjct: 1021 VPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4189RTXTOXIND453e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.8 bits (106), Expect = 3e-07
Identities = 20/93 (21%), Positives = 36/93 (38%), Gaps = 2/93 (2%)

Query: 73 EVRPQVTGILLERQFQEGSEVKAGQVLYQINPAPFRATLSRAQASLDSAKLLADRYDRLI 132
E++P I+ E +EG V+ G VL ++ A + Q+SL A+L RY L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 133 ETRAISQQERDDARSQ--YLQARAAVESARIDL 163
+ +++ + + L
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSL 190



Score = 37.1 bits (86), Expect = 9e-05
Identities = 14/86 (16%), Positives = 33/86 (38%), Gaps = 3/86 (3%)

Query: 108 RATLSRAQASLDSAKLLADRYDRLIETRAISQQERDDARSQYLQARAAVESARIDLDFTR 167
++ L + ++ + SAK +L + + + + +
Sbjct: 272 KSQLEQIESEILSAKEEYQLVTQLFKNEILDK--LRQTTDNIGLLTLELAKNEERQQASV 329

Query: 168 ITAPISGRIGRSSV-TQGALVTANQA 192
I AP+S ++ + V T+G +VT +
Sbjct: 330 IRAPVSVKVQQLKVHTEGGVVTTAET 355


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4190HTHTETR566e-12 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.8 bits (134), Expect = 6e-12
Identities = 27/181 (14%), Positives = 58/181 (32%), Gaps = 11/181 (6%)

Query: 13 AKRRKEQVITAAAECVRREGFHRTSMSQISAAAGMSAGHIHHFFGGKDGIIAGIVAREHT 72
A+ ++ ++ A ++G TS+ +I+ AAG++ G I+ F K + + I +
Sbjct: 9 AQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSES 68

Query: 73 ELAQLIEDV--RSSSQGSDAVTAIVKELPRSVPRYMDPGRAALTMEILAEASR-NSEVAH 129
+ +L + + + I+ + S + E + V
Sbjct: 69 NIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQ 128

Query: 130 LIQENDVEVRHAFRDLLGN--------RASDIEARCEIVGALLEGLSARTLRNPQLSTMV 181
+ +E L + I+ + GL L PQ +
Sbjct: 129 AQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLK 188

Query: 182 N 182

Sbjct: 189 K 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4191HTHTETR612e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 61.2 bits (148), Expect = 2e-13
Identities = 35/173 (20%), Positives = 57/173 (32%), Gaps = 10/173 (5%)

Query: 20 MKKRGRQPDPAKAQVILEAACSSFSHRGYFGTSMETIAACAHTTKATIYAKFDSKERLFA 79
+K ++ + IL+ A FS +G TS+ IA A T+ IY F K LF+
Sbjct: 2 ARKTKQEAQETRQH-ILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 80 AALEELERRM-PRPQDIMRCSGKDVLDDLLVIASRLLKLALHRSTLGIYRMLLLPIDHAP 138
E E + + D L L I +L+ + R LL+ I
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEER----RRLLMEIIFHK 116

Query: 139 RLGAQFWQKIVEPYRKAMEEVLRDAH----RCQSLHIIDPRLASDHFFSLVIG 187
+ + R E C ++ L + ++ G
Sbjct: 117 CEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRG 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4195IGASERPTASE300.010 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.0 bits (67), Expect = 0.010
Identities = 21/77 (27%), Positives = 30/77 (38%), Gaps = 3/77 (3%)

Query: 116 PLAAPADAAREPTRKRAELARSGPAALQEIADAIVKAATSAETKAERPVALALVRESVMR 175
P AD P+ E+AR A + A A + + ET AE + E +
Sbjct: 1000 PNNIQADVPSVPSN-NEEIARVDEAPVPPPAPAT--PSETTETVAENSKQESKTVEKNEQ 1056

Query: 176 QPPEGYARNCEALAEAQ 192
E A+N E EA+
Sbjct: 1057 DATETTAQNREVAKEAK 1073


104Bpet4467Bpet4472N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4467021-3.204633outer membrane usher protein
Bpet4468-124-3.313137putative fimbrial adhesin
Bpet4469019-1.234433virulence sensor protein
Bpet4470-1140.528779virulence sensor protein
Bpet4471-2110.409216virulence factors transcription regulator
Bpet4472-2131.201948virulence sensor protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4467PF005777700.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 770 bits (1989), Expect = 0.0
Identities = 282/873 (32%), Positives = 420/873 (48%), Gaps = 40/873 (4%)

Query: 11 LRYACGLVSALFACGIGASVAAEASSAQVAEVQFNTDMLRGFGDAPVDISRFNRGNFAAP 70
L ++ F A A + AE+ FN L A D+SRF G P
Sbjct: 16 LHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPP 75

Query: 71 GDYTVPIVVNDRRVGRGTVRLRQLAGEAYPQPCVDTDLLTTAGVNVQRLDDAAQAQLQEN 130
G Y V I +N+ + V E PC+ L + G+N + ++
Sbjct: 76 GTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLA--DD 133

Query: 131 SCVRLPGLIPDARAEFDNGEQHLYLSIPQIWLNRSARGYVDPDHWNEGITAGMLRYNANV 190
+CV L +I DA A+ D G+Q L L+IPQ +++ ARGY+ P+ W+ GI AG+L YN +
Sbjct: 134 ACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSG 193

Query: 191 YRYNSRHGSASTQGYLGLDSGFNVGAWRFRHRGNLSYQENLG-----THYESIQTSVQRS 245
+R G S YL L SG N+GAWR R SY + ++ I T ++R
Sbjct: 194 NSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERD 253

Query: 246 LAPIKSQLTAGEFFTEGDVLESLNLRGVRLSSDDRMYPESLRGYAPTVHGIANSNARVSI 305
+ P++S+LT G+ +T+GD+ + +N RG +L+SDD M P+S RG+AP +HGIA A+V+I
Sbjct: 254 IIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTI 313

Query: 306 RQNGIVIYETTVAPGEFQIDDLYPTGYGGDLEVVVTEADGSVHISRVPFSAPINALRAGA 365
+QNG IY +TV PG F I+D+Y G GDL+V + EADGS I VP+S+ R G
Sbjct: 314 KQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGH 373

Query: 366 TRYSLAAGQYRNTMG-GETPYVFQGTVRHGFNNLVTGYGGITASEHYLAGEVGAALNT-S 423
TRYS+ AG+YR+ E P FQ T+ HG T YGG ++ Y A G N +
Sbjct: 374 TRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGA 433

Query: 424 WGAFSLDATYARARLRNQPDRRGQSYGLSYSRLYEPTATSVTLAAYRYSTDGFLNLADTV 483
GA S+D T A + L + GQS Y++ + T++ L YRYST G+ N ADT
Sbjct: 434 LGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTT 493

Query: 484 ALRSAD--------------SLYALPRGYGSAKGRLQVMLNQPLGERWGSLYLSGYSQNY 529
R + +G+LQ+ + Q LG R +LYLSG Q Y
Sbjct: 494 YSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLG-RTSTLYLSGSHQTY 552

Query: 530 WGHSGRDTEYQAGYSNSFKRVNYNISASRQYSAYSGKWENTYMLNFSLPLGSGANAPR-- 587
WG S D ++QAG + +F+ +N+ +S S +A+ + LN ++P +
Sbjct: 553 WGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKS 612

Query: 588 ------SNTTIQRNNRTRSTFIYETVNGSLGGSDSPLYYGVSASHSRHGGQGANSNNVSA 641
++ ++ + R T V G+L D+ L Y V ++ GG G + + A
Sbjct: 613 QWRHASASYSMSHDLNGRMT-NLAGVYGTL-LEDNNLSYSVQTGYAG-GGDGNSGSTGYA 669

Query: 642 NASWTSPLAQLGASASRSSNSSQASASISGAAVAWGGGVALTPSLGDTFAIVDAQGAAGA 701
++ S S + Q +SG +A GV L L DT +V A GA A
Sbjct: 670 TLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDA 729

Query: 702 RIANMGGLRVNSRGYGVVSNLTPFAQNTIEVDPNGLPLNVQFKSTIQHVAPTAGAIVPVK 761
++ N G+R + RGY V+ T + +N + +D N L NV + + +V PT GAIV +
Sbjct: 730 KVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAE 789

Query: 762 FEVEAGGQAAVIRARQADGQALPFGAQALDGNGNQVGTVAQGSRIIASSLKDTKGRITIK 821
F+ G ++ + + LPFGA + G VA ++ S + G++ +K
Sbjct: 790 FKARVG--IKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPL-AGKVQVK 846

Query: 822 WGATAAQQCTVDYALPEAAGKADQPFHLLQGTC 854
WG C +Y LP Q L C
Sbjct: 847 WGEEENAHCVANYQLPPE--SQQQLLTQLSAEC 877


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4469HTHFIS738e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 73.3 bits (180), Expect = 8e-16
Identities = 34/120 (28%), Positives = 50/120 (41%), Gaps = 3/120 (2%)

Query: 477 ILVVDDHAANRTLLKHQLQKLGHAVVCAENGLQALEAVGRQVFKLAICDCAMPRMNGMEF 536
ILV DD AA RT+L L + G+ V N + L + D MP N +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 537 VRALRAGPEPNARMPVLGYTAGAQDNYAQQAIDAGMDAVLFKPAGLAELQAALRAHLPQP 596
+ ++ + +PVL +A A +A + G L KP L EL + L +P
Sbjct: 66 LPRIK---KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4471HTHFIS762e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.0 bits (187), Expect = 2e-18
Identities = 35/119 (29%), Positives = 62/119 (52%), Gaps = 2/119 (1%)

Query: 2 TSVLIVDDHPSLRLILRQQLSQMLGVSQIIEAGNGQDAVQAVRQHEPGLVILDIDLPKIN 61
++L+ DD ++R +L Q LS+ + N + + + LV+ D+ +P N
Sbjct: 4 ATILVADDDAAIRTVLNQALSRA--GYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 62 GLEAIPRIKLIKPDIRILVISAQDPVVFAPRVKAAGAQGYISKVQELPEIVRAIETVLA 120
+ +PRIK +PD+ +LV+SAQ+ + A + GA Y+ K +L E++ I LA
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4472HTHFIS731e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 73.3 bits (180), Expect = 1e-15
Identities = 29/109 (26%), Positives = 48/109 (44%), Gaps = 5/109 (4%)

Query: 561 QTARVLAVDDHEPYRIILRQLMLRAGLNCDTVADAEQALEALRQHDYAMLFTDCQMPGID 620
A +L DD R +L Q + RAG + ++A + D ++ TD MP +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 621 GCELARRVRQQEAQAQRPRLPIVGVTADCSTQQMQRCRASGMDDYLAKP 669
+L R++ RP LP++ ++A + + G DYL KP
Sbjct: 62 AFDLLPRIK-----KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKP 105


105Bpet4506Bpet4515N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4506-1181.688697hypothetical protein
Bpet4507-1201.833776LysR family transcriptional regulator
Bpet4508-1222.225822HlyD family secretion protein
Bpet4509-2211.987522AcrB/AcrD/AcrF family protein
Bpet45101183.933912putative outer membrane efflux protein
Bpet45110173.171891putative 2'-5' RNA ligase
Bpet4512-1172.489483putative 5'(3')-deoxyribonucleotidase
Bpet4513-1172.012307hypothetical protein
Bpet45141152.391363LysR family transcriptional regulator
Bpet45151152.685074putative transmembrane efflux protein of the MFS
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4506PF04183290.032 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 29.1 bits (65), Expect = 0.032
Identities = 40/176 (22%), Positives = 58/176 (32%), Gaps = 24/176 (13%)

Query: 123 GMAVPDWISSLPAVGPRLAVYWQTYLGEPHALGALVELVSG---------EHLGNIYRMV 173
G W+ + A L LGEP A E + E LG I+R
Sbjct: 295 GPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRE- 353

Query: 174 LSATGNAFQLLLN--VVFMLITLFFVYKDGDRMIAQLDVLGERILPTRWQR--FSRVVPA 229
N + L ++ TL ++ + + W F VV
Sbjct: 354 -----NPCRWLKPDESPVLMATLMECDENNQPLAGAYIDR-SGLDAETWLTQLFRVVVVP 407

Query: 230 TVGS-TVTGMSLIAVGEGVVLGVAYWLAGVPSPVLLGVVTGFMALIPGGAPLSFTL 284
G++LIA G+ + L + GVP VLL G M L+ P +L
Sbjct: 408 LYHLLCRYGVALIAHGQNITLAMK---EGVPQRVLLKDFQGDMRLVKEEFPEMDSL 460


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4508RTXTOXIND522e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.8 bits (124), Expect = 2e-09
Identities = 18/98 (18%), Positives = 36/98 (36%), Gaps = 5/98 (5%)

Query: 110 AEVDRAAAQLAAARARVAFTASELARGK-RLLAENAIARRDFESKRNDAREAAANLQAAE 168
+ A +L ++++ SE+ K + + + K + N+
Sbjct: 259 NKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTD---NIGLLT 315

Query: 169 AALDAAKLNLGYTEIVAPVDGRVSRAEI-TEGNVVAAG 205
L + + I APV +V + ++ TEG VV
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353



Score = 50.2 bits (120), Expect = 8e-09
Identities = 26/148 (17%), Positives = 47/148 (31%), Gaps = 31/148 (20%)

Query: 49 VAPALGKTIVDWQDYSGRLEAIDRVDIRPLVSGTLTAVHFQDGSLVHKGDPLFTIDPRPY 108
VA A GK SGR +I+P+ + + + ++G V KGD L +
Sbjct: 83 VATANGKLTH-----SGR-----SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGA 132

Query: 109 AAEVDRAAAQLAAARARVA---------------------FTASELARGKRLLAENAIAR 147
A+ + + L AR + + +L ++ +
Sbjct: 133 EADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIK 192

Query: 148 RDFESKRNDAREAAANLQAAEAALDAAK 175
F + +N + NL A
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVL 220


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4509ACRIFLAVINRP10980.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1098 bits (2841), Expect = 0.0
Identities = 436/1042 (41%), Positives = 663/1042 (63%), Gaps = 16/1042 (1%)

Query: 3 ISKFFIDRPIFAGVLSVIVLLAGLLAMFQLPISEYPEVVPPSVVVRAQYPGANPKVIAAT 62
++ FFI RPIFA VL++I+++AG LA+ QLP+++YP + PP+V V A YPGA+ + + T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VASPLEESINGVEDMLYMQSQANSDGNLAVTVYFKLGVDPDKAQQLVQNRVSQALPRLPP 122
V +E+++NG+++++YM S ++S G++ +T+ F+ G DPD AQ VQN++ A P LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 123 DVQRLGVTTTKSSPTLTLVVHLISPNDRYDITYLRNYAVLNVKDRLSRIGGVGEVQIWGS 182
+VQ+ G++ KSS + +V +S N + +Y NVKD LSR+ GVG+VQ++G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 183 GSYSMRVWLDPQKVAQRGLTATDVVNAIREQNVQVAAGVIGASPTQGDVPMQFSVNAQGR 242
Y+MR+WLD + + LT DV+N ++ QN Q+AAG +G +P + S+ AQ R
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 243 LQNETEFGNIILKSSPDGAVTRLSDVARIELGAQEYGLRSLLNNKPAIGMGIMQSPGANA 302
+N EFG + L+ + DG+V RL DVAR+ELG + Y + + +N KPA G+GI + GANA
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 303 LDVSAQVRETMKELSADFPPGLEYRIEYDPTQFVRSSIKAVISTLLEAIALVVLVVIVFL 362
LD + ++ + EL FP G++ YD T FV+ SI V+ TL EAI LV LV+ +FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 363 QTWRASIIPLLAVPVSIVGTFSLLLLFGYSINALSLFGMVLAIGIVVDDAIVVVENVERN 422
Q RA++IP +AVPV ++GTF++L FGYSIN L++FGMVLAIG++VDDAIVVVENVER
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 423 IA-AGLTPREATYRAMREVSGPIIAIALTLCAVFVPLAFMTGLSGQFYKQFAMTIAISTV 481
+ L P+EAT ++M ++ G ++ IA+ L AVF+P+AF G +G Y+QF++TI +
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 482 ISAFNSLTLSPALSALLLKGHHDKPDWLTRGMNRVFGGFFNWFNRFFGRASDSYATGITG 541
+S +L L+PAL A LLK ++ + GGFF WFN F + + Y +
Sbjct: 480 LSVLVALILTPALCATLLKP-------VSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGK 532

Query: 542 VIRRKGGAMAVYAVLLAATVGISYLVPGGFVPAQDKQYLIGFAQLPNGASLDRTEDVIRR 601
++ G + +YA+++A V + +P F+P +D+ + QLP GA+ +RT+ V+ +
Sbjct: 533 ILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQ 592

Query: 602 MSDIALK--EPGVESAIAFPGLSINGFTNSSSAGIVFVTLKPFDERHSAELSGNAITGSL 659
++D LK + VES G S +G + +AG+ FV+LKP++ER+ E S A+
Sbjct: 593 VTDYYLKNEKANVESVFTVNGFSFSG--QAQNAGMAFVSLKPWEERNGDENSAEAVIHRA 650

Query: 660 NAKFASIKDAFIAVFPPPPVMGLGTMGGFKLQIEDRAALGYAELDKATQAFLAKARQAP- 718
+ I+D F+ F P ++ LGT GF ++ D+A LG+ L +A L A Q P
Sbjct: 651 KMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPA 710

Query: 719 ELGPTFSNYQINVPQLDVDLDRVKAKQLGVPVTDVFDTLQIYLGSMYVNDFNRFGRVFQV 778
L N + Q +++D+ KA+ LGV ++D+ T+ LG YVNDF GRV ++
Sbjct: 711 SLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKL 770

Query: 779 RAQADAPFRAHPDDILQLKTRSDSGQMVPLSALVDVKQTFGPEMVVRYNGYTAADINGGP 838
QADA FR P+D+ +L RS +G+MVP SA +G + RYNG + +I G
Sbjct: 771 YVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA 830

Query: 839 APGYSSDQAQDAAERIAAETLPRGVKFEWTDLTYQQILAGNAGIWVFPISVLLVFLVLAA 898
APG SS A E +A++ LP G+ ++WT ++YQ+ L+GN + IS ++VFL LAA
Sbjct: 831 APGTSSGDAMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAA 889

Query: 899 LYESLTLPLAVILIVPMSILAALTGVWLTSGDNNIFTQIGLMVLVGLSAKNAILIVEFAR 958
LYES ++P++V+L+VP+ I+ L L + N+++ +GL+ +GLSAKNAILIVEFA+
Sbjct: 890 LYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAK 949

Query: 959 EL-EMQGSTPLQAAIEASRLRLRPILMTSIAFIMGVVPLVLSSGAGSEMRHAMGVAVFFG 1017
+L E +G ++A + A R+RLRPILMTS+AFI+GV+PL +S+GAGS ++A+G+ V G
Sbjct: 950 DLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGG 1009

Query: 1018 MLGVTLFGLFLTPVFYVLLRTL 1039
M+ TL +F PVF+V++R
Sbjct: 1010 MVSATLLAIFFVPVFFVVIRRC 1031



Score = 89.9 bits (223), Expect = 3e-20
Identities = 64/325 (19%), Positives = 127/325 (39%), Gaps = 14/325 (4%)

Query: 733 QLDVDLDRVKAKQLGVPVTDVFDTL-----QIYLGSMYVNDFNRFGRVFQVRAQADAPFR 787
+ + LD + + DV + L QI G G+ A F+
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ-LGGTPALPGQQLNASIIAQTRFK 241

Query: 788 AHPDDILQLKTRSD-SGQMVPLSALVDVKQTFGP-EMVVRYNGYTAADINGGPAPGYSS- 844
P++ ++ R + G +V L + V+ ++ R NG AA + A G ++
Sbjct: 242 N-PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 845 DQAQDAAERIA--AETLPRGVKFEWT-DLTYQQILAGNAGIWVFPISVLLVFLVLAALYE 901
D A+ ++A P+G+K + D T L+ + + +++LVFLV+ +
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 902 SLTLPLAVILIVPMSILAALTGVWLTSGDNNIFTQIGLMVLVGLSAKNAILIVE-FAREL 960
++ L + VP+ +L + N T G+++ +GL +AI++VE R +
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 961 EMQGSTPLQAAIEASRLRLRPILMTSIAFIMGVVPLVLSSGAGSEMRHAMGVAVFFGMLG 1020
P +A ++ ++ ++ +P+ G+ + + + M
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 1021 VTLFGLFLTPVFYVLLRTLSARKLH 1045
L L LTP L + + H
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHH 505


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4510RTXTOXIND349e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 34.4 bits (79), Expect = 9e-04
Identities = 23/192 (11%), Positives = 56/192 (29%), Gaps = 36/192 (18%)

Query: 74 TLNALETQAQQANHSLQAAAARLKQAR--ALLGNARSEQFPTVDAGFGPTRQRPSPASQG 131
L AL +A ARL+Q R L + + P + P Q
Sbjct: 126 KLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKL-------PDEPYFQN 178

Query: 132 LSANDSTDPSTLWRAQVGVSYEVDLFGRVASTVDAATADVQQSEALYRSVLLALQADVAQ 191
+S + V T+ +++ + +++ + ++ +
Sbjct: 179 VSEEE---------------------------VLRLTSLIKEQFSTWQNQKYQKELNLDK 211

Query: 192 AYFQVRELDAGLQLYRQTVELRAETLQLIQRRYDAGDISELDLARARSELESARSEALGF 251
+ + A + Y + L I++ + ++ A +E +
Sbjct: 212 KRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVY 271

Query: 252 ERRRANAEHALA 263
+ + E +
Sbjct: 272 KSQLEQIESEIL 283


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4515TCRTETB491e-08 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 49.5 bits (118), Expect = 1e-08
Identities = 56/331 (16%), Positives = 111/331 (33%), Gaps = 50/331 (15%)

Query: 26 LPEVGADLGVSLSSAGLLVTGYALGVVVGAPPVAILTTRMPRKTLLLALMVIFTLGNLAC 85
LP++ D +S + T + L +G L+ ++ K LLL ++I G++
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 86 ALAPGYGT-LMAARVLTSLAHGAFFGVGSVVATSLVKPEKQASAIALMFTGLTLANVLGV 144
+ + + L+ AR + AF + VV + E + A L+ + + + +G
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 145 PFGTWLGQAWGWRATFWAVTVVGIVAMLAIATWVPRSRGDRGGDLMG------------- 191
G + W + I + R D+ G
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFML 216

Query: 192 ----------------------ELRALSRPQV----------LLGFAMTVLGFGGVFTAF 219
+R ++ P V ++G + FG V
Sbjct: 217 FTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV 276

Query: 220 TYIAPLLTELAGFSPGAVSPILLLFGVGLVAGNTY-GGKLADR---RLMPTLVGSLALLA 275
+ + ++ ++ S + +++ G V Y GG L DR + + + ++
Sbjct: 277 SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVS 336

Query: 276 IVLAVFSLTVHAQFAAVATVAVLGAAAFATV 306
+ A F L + F + V VLG +F
Sbjct: 337 FLTASFLLETTSWFMTIIIVFVLGGLSFTKT 367


106Bpet4591Bpet4596N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4591353-11.261088copper resistance protein A precursor
Bpet4592255-11.690240copper tolerance protein
Bpet4593257-12.219728two-component sensor kinase
Bpet4594258-12.153788two-component response regulator
Bpet4595153-10.704913hypothetical protein
Bpet4596047-8.843740putative glycosylttransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4591cloacin320.008 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 32.0 bits (72), Expect = 0.008
Identities = 27/114 (23%), Positives = 42/114 (36%), Gaps = 7/114 (6%)

Query: 375 AHDMSGMDMGGESGGSMKG--MDHGSMSNADQSSSGAGNQGAMSGMDHGSMGGMAGMSHG 432
AH SG GG +G + G D S+ + G G G G G + G
Sbjct: 13 AHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSG 72

Query: 433 GMNHGAGDGEGGMVEVKHPYPAENSP-----ATTMPPDVVSTRLDDPGVGLRGN 481
G + G+ V +PA ++P A ++ +S + D L+G
Sbjct: 73 GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKGP 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4594HTHFIS788e-19 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 77.6 bits (191), Expect = 8e-19
Identities = 41/126 (32%), Positives = 65/126 (51%), Gaps = 1/126 (0%)

Query: 2 RILVIEDERKLAHYLQKGLTEHNYVVDIASNGVDGRHAALEGNYDLVVLDVMLPGIDGFW 61
ILV +D+ + L + L+ Y V I SN G+ DLVV DV++P + F
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 ILKDLRET-KDTPVLMLTARDKVEDRVRGLENGADDYLVKPFAFSELLARIQALLRRGRG 120
+L +++ D PVL+++A++ ++ E GA DYL KPF +EL+ I L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 121 QESTLL 126
+ S L
Sbjct: 125 RPSKLE 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4595ENTEROVIROMP280.008 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 27.9 bits (62), Expect = 0.008
Identities = 16/46 (34%), Positives = 20/46 (43%), Gaps = 4/46 (8%)

Query: 1 MKRISCAIVAAAISFGAIGIVHAETKT----RAQVRAELQEAKAKG 42
MK+I+C AA+ G A T T AQ A+ Q K G
Sbjct: 1 MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKMGG 46


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4596PF06057280.045 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 28.3 bits (63), Expect = 0.045
Identities = 16/130 (12%), Positives = 40/130 (30%), Gaps = 20/130 (15%)

Query: 178 VRHYRKRWIEPRRSGRLVVGSNAGTADYKRWLDMVEGA-ALLPKHLRDQI--IILIAGVP 234
+ Y+ + +++G Y +++ +P R + +L++
Sbjct: 107 IDKYQAEF---GTQKVILIG-------YSFGAEVIPFVLNEMPARYRKNVLGAVLLSPSQ 156

Query: 235 PSEDQVSKVEQLGMMDHVIFVGLLDDVRPFIATLDVGFVL---SSEVETISFACREMMAM 291
S+ ++ E + + P + +L E + C E+
Sbjct: 157 SSDFEIHVSEMVTSDNQ----SARYLTLPEVNKQTTVPMLCLYGKEDDAPLHLCPEVKQP 212

Query: 292 GVPVIVSDSG 301
V V+ G
Sbjct: 213 NVTVMELSGG 222


107Bpet4606Bpet4615N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4606464-12.226708AcrB/AcrD/AcrF family transporter
Bpet4607464-12.260706cobalt-zinc-cadmium resistance protein czcB
Bpet4608365-13.362389metal ion efflux outer membrane protein,
Bpet4609156-12.333899two-component response regulator
Bpet4610145-9.044006two-component sensor kinase
Bpet4611138-6.941297two-component response regulator
Bpet4612133-5.225825Outer membrane porin protein precursor
Bpet4613229-3.701950hypothetical protein
Bpet4614328-2.839859LysR family transcriptional regulator
Bpet4615123-1.928499conjugal transfer coupling protein TraG
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4606ACRIFLAVINRP6490.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 649 bits (1677), Expect = 0.0
Identities = 224/1077 (20%), Positives = 413/1077 (38%), Gaps = 78/1077 (7%)

Query: 8 LSVRARWAVLFLFLAIGALGVWQLTKLPIDAVPDITNNQVQINTVDPRLSPVEIEKLVTY 67
+R L + + G + +LP+ P I V ++ P ++ VT
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 68 PVEISLAGIPGLESTRSIS-RNGFSQVTAIFTDKTDLYFARQQVGERLIKAQESLPDGVQ 126
+E ++ GI L S S G +T F TD A+ QV +L A LP VQ
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 127 PQIGPVTTGLGEVLLYTVGYTYPDGEGAQKVAGEPGWQPDGSYLTPEGDRMVDEVAKAGY 186
Q V L+ G+ + Q
Sbjct: 124 QQGISVEKSSSSYLMV-AGFVSDNPGTTQ-----------------------------DD 153

Query: 187 LRTVQDWIVAPQLKALPGVAGVDSIGGYAKTFVVEPNPTKLASYGISYSELGEALERANI 246
+ V L L GV V G + + L Y ++ ++ L+ N
Sbjct: 154 ISDYVASNVKDTLSRLNGVGDVQLFGA-QYAMRIWLDADLLNKYKLTPVDVINQLKVQND 212

Query: 247 AVGANYYNRGGEA------YLVRVDARVGSVDEIRN-AVAATRGGVPITVGQIADVKIGG 299
+ A + R + +E + G + + +A V++GG
Sbjct: 213 QIAAGQLGGTPALPGQQLNASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGG 272

Query: 300 DLRTGAGSMNGEEAVIGTVLMLIGENSRVVAEDVSAKLDQIATSLPPGIQVKTVLDRAKL 359
+ +NG+ A + + G N+ A+ + AKL ++ P G++V D
Sbjct: 273 ENYNVIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPF 332

Query: 360 VNATVSTVERNLTEGAILVAASLFLLLGNWRAALIAVLVIPFSFLMMAMGMNAFKVPGNL 419
V ++ V + L E +LV ++L L N RA LI + +P L + AF N
Sbjct: 333 VQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT 392

Query: 420 MSLG--ALDFGLIVDGSVIIIENCLARLAHRQQHEGRLLFLRERLEETMRAAQEMIKPTV 477
+++ L GL+VD +++++EN R E +L E T ++ ++ V
Sbjct: 393 LTMFGMVLAIGLLVDDAIVVVENV-----ERVMMEDKL----PPKEATEKSMSQIQGALV 443

Query: 478 FGQVIILLTFAPLLMFTGVEGKTFSPMAITIMLALVAAFILAITLVPALVAILIRGRVAE 537
+++ F P+ F G G + +ITI+ A+ + ++A+ L PAL A L++ AE
Sbjct: 444 GIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAE 503

Query: 538 KE-------VWLI---AKSKERYLPFLDKAIARPWPFIFAGLVFFLAAIPAFGLLGSEFI 587
W S Y + K + ++ + + F L S F+
Sbjct: 504 HHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFL 563

Query: 588 PKLDEKNLAVASTRVPSVSLEQSLAMQLKVEDAIKKLPEVELMFSKTGTAEVATDPMPPN 647
P+ D+ + E++ + +V D K + + S + N
Sbjct: 564 PEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANV-ESVFTVNGFSFSGQAQN 622

Query: 648 VSDGFVILKPQEEWPDGVTTKAQVIERV-EKAAGTQLGNLYEVSQPIELRFNELIAGVRG 706
FV LKP EE + VI R + + G + + P EL
Sbjct: 623 AGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMP---AIVELGTATGF 679

Query: 707 DVA-IKLYGDDLEKMQQTANEMVRVLQDIPGA-GSVKADQVGGAPTLDVKLNRAEIARYG 764
D I G + + Q N+++ + P + SV+ + + +++++ + G
Sbjct: 680 DFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALG 739

Query: 765 LTVQDVADTIAAALGGRPSGLLYEGDRRFDITVRVPEATRMNLDAIRALPILLPEMEGQL 824
+++ D+ TI+ ALGG + R + V+ RM + + L + G++
Sbjct: 740 VSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYV--RSANGEM 797

Query: 825 RRQVPLARVAQIRLTEGLNEIRRENGKRRVVVQVNLDGRDAGSFVEEAQAKIAQV--QLP 882
VP + G + R NG + +Q G+ +A A + + +LP
Sbjct: 798 ---VPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA---APGTSSGDAMALMENLASKLP 851

Query: 883 AGYYLEWGGQFESLQAASQRLSIVVPICFLAIFVLLFMALGGFGRALSVFLAVPLGLAGG 942
AG +W G + + + +V I F+ +F+ L + +SV L VPLG+ G
Sbjct: 852 AGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGV 911

Query: 943 VFTLAMTGINFSVSAAVGFICLAGVAVLNGLVVMT-AIRAHTEAGLPLSEAIREGMKEKM 1001
+ + V VG + G++ N ++++ A + G + EA ++ ++
Sbjct: 912 LLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRL 971

Query: 1002 RAVVMTGFVPAIGFVPMALALGTGAEVQKPLATTVIGGLIAATILTLLVLPAIAKVV 1058
R ++MT +G +P+A++ G G+ Q + V+GG+++AT+L + +P V+
Sbjct: 972 RPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVI 1028



Score = 109 bits (274), Expect = 3e-26
Identities = 101/534 (18%), Positives = 202/534 (37%), Gaps = 46/534 (8%)

Query: 557 AIARPWPFIFAGLVFFLAAIPAFGLLGSEFIPKLDEKNLAVASTRVPSVS---LEQSLAM 613
I RP ++ +A A L P + ++V S P ++ ++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSV-SANYPGADAQTVQDTVTQ 63

Query: 614 QLKVEDAIKKLPEVELMFSKTGTAEVATDPMPPNVSDGFVILKPQEEWPDGVTTKAQVIE 673
+E + + + M S + +A T ++ F + D + QV
Sbjct: 64 --VIEQNMNGIDNLMYMSSTSDSAGSVT------ITLTF------QSGTDPDIAQVQVQN 109

Query: 674 RVEKAAGTQLGNLYEVSQPIELRFNELIAGVRGDVAIKLYGDDLEKMQQT---ANEMVRV 730
+++ A L + Q + + + + + A+ +
Sbjct: 110 KLQLA----TPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDT 165

Query: 731 LQDIPGAGSVKADQVGGAPTLDVKLNRAEIARYGLTVQDVADTIAAAL----GGRPSGLL 786
L + G G V G + + L+ + +Y LT DV + + G+ G
Sbjct: 166 LSRLNGVGDV--QLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTP 223

Query: 787 YEGDRRFDITVRVPEATRMNLDAIRALPILLPEMEGQLRRQVPLARVAQIRL-TEGLNEI 845
++ + ++ + N + + + V L VA++ L E N I
Sbjct: 224 ALPGQQLNASIIA-QTRFKNPEEFGKVTLR----VNSDGSVVRLKDVARVELGGENYNVI 278

Query: 846 RRENGKRRVVVQVNL-DGRDAGSFVEEAQAKIAQVQ--LPAGYYLEWGGQFESLQAASQR 902
R NGK + + L G +A + +AK+A++Q P G ++ +++
Sbjct: 279 ARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQG--MKVLYPYDTTPFVQLS 336

Query: 903 LSIVVPICFLAI---FVLLFMALGGFGRALSVFLAVPLGLAGGVFTLAMTGINFSVSAAV 959
+ VV F AI F+++++ L L +AVP+ L G LA G + +
Sbjct: 337 IHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMF 396

Query: 960 GFICLAGVAVLNGLVVMTAI-RAHTEAGLPLSEAIREGMKEKMRAVVMTGFVPAIGFVPM 1018
G + G+ V + +VV+ + R E LP EA + M + A+V V + F+PM
Sbjct: 397 GMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPM 456

Query: 1019 ALALGTGAEVQKPLATTVIGGLIAATILTLLVLPAIAKVVLEPKEKRKSDIPEG 1072
A G+ + + + T++ + + ++ L++ PA+ +L+P + G
Sbjct: 457 AFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGG 510



Score = 88.0 bits (218), Expect = 1e-19
Identities = 52/322 (16%), Positives = 116/322 (36%), Gaps = 24/322 (7%)

Query: 218 FVVEPNPTKLASYGISYSELGEALERANIAVGANYYNRGGEAYLVRV---DARVGSVDEI 274
F +E + K + G+S S++ + + A N + G + V +++
Sbjct: 726 FKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDV 785

Query: 275 RNAVAATRGGVPITVGQIADVKIGGDLRTGAGSMNGEEAVIGTVLMLIGENSRVVAEDVS 334
+ G + G+ + + + + D
Sbjct: 786 DKLYVRSANGEMVPFSAFTTSHWV----YGSPRLERYNGLPSMEI-QGEAAPGTSSGDAM 840

Query: 335 AKLDQIATSLPPG--IQVKTVLDRAKLVNATVSTVERNLTEGAILVAASLFLLLGNWRAA 392
A ++ +A+ LP G + + +L + + ++V L L +W
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPAL---VAISFVVVFLCLAALYESWSIP 897

Query: 393 LIAVLVIPFSFLMMAMGMNAFKVPGNLMSLGAL--DFGLIVDGSVIIIENCLARLAHRQQ 450
+ +LV+P + + + F ++ + L GL +++I+E +
Sbjct: 898 VSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVE----FAKDLME 953

Query: 451 HEGRLLFLRERLEETMRAAQEMIKPTVFGQVIILLTFAPLLMFTGVEGKTFSPMAITIML 510
EG+ + E T+ A + ++P + + +L PL + G + + I +M
Sbjct: 954 KEGKGVV-----EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008

Query: 511 ALVAAFILAITLVPALVAILIR 532
+V+A +LAI VP ++ R
Sbjct: 1009 GMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4607RTXTOXIND492e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 48.7 bits (116), Expect = 2e-08
Identities = 33/200 (16%), Positives = 60/200 (30%), Gaps = 30/200 (15%)

Query: 125 ASRDAAQLAAARTAAYARAELARKELAREQYLYKQQVSARVDLERAQAEAQAAAADARRA 184
A + + + A++E L+K ++ + Q A
Sbjct: 264 AVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILD----KLRQTTDNIGLLTLELA 319

Query: 185 KVEAEVANVTKDGQGVAVSSPISGRITTQSL-SLGAFVQPETELFRIA-DPKQIQVEAAI 242
K E + +P+S ++ + + G V L I + ++V A +
Sbjct: 320 KNEERQQASV-------IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 243 LPSDIFRIAPGDRAIVELPG-----GGTLEAKVGSVTPSLNTATRQ------------AT 285
DI I G AI+++ G L KV ++ R
Sbjct: 373 QNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENC 432

Query: 286 AVIDVEAGSLQPGLAVRVRI 305
+ L G+AV I
Sbjct: 433 LSTGNKNIPLSSGMAVTAEI 452



Score = 36.7 bits (85), Expect = 1e-04
Identities = 22/141 (15%), Positives = 48/141 (34%), Gaps = 17/141 (12%)

Query: 76 LDAEIGAQAVVSPQPGGEAIVTARASGAVTQVFKRLGDPVQAGEVLA-VVASRDAAQLAA 134
++ A ++ G + + V ++ + G+ V+ G+VL + A A
Sbjct: 80 VEIVATANGKLTHS-GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 135 ARTA-AYARAELARKELAREQ-------------YLYKQQVSAR-VDLERAQAEAQAAAA 179
+++ AR E R ++ Y Q VS V + + Q +
Sbjct: 139 TQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTW 198

Query: 180 DARRAKVEAEVANVTKDGQGV 200
++ + E + + V
Sbjct: 199 QNQKYQKELNLDKKRAERLTV 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4609HTHFIS877e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 7e-22
Identities = 40/142 (28%), Positives = 74/142 (52%), Gaps = 4/142 (2%)

Query: 1 MGKLKILVIEDERKLAEYLKRALSEHNYVVDIAMDGISGLHLAQETQYDLILLDVMLPGR 60
M ILV +D+ + L +ALS Y V I + + DL++ DV++P
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 DGFSVLAELRKGD-RVPILMLTARDKLEDRVRGLQDGADDYLAKPFALSELLA---RVLA 116
+ F +L ++K +P+L+++A++ ++ + GA DYL KPF L+EL+ R LA
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 117 LSRRQSNSVIEPNRQNVLKVGD 138
+R+ + + + ++ + VG
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGR 142


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4611HTHFIS672e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.8 bits (163), Expect = 2e-15
Identities = 27/98 (27%), Positives = 53/98 (54%), Gaps = 5/98 (5%)

Query: 2 QYDLIILDINLPDMEGFEVLQRIRQSDA-VPVMMLTARTSLEDRVRGLEQGADDYLAKPF 60
DL++ D+ +PD F++L RI+++ +PV++++A+ + ++ E+GA DYL KPF
Sbjct: 47 DGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106

Query: 61 ALSELQARVQALRRRGSGNESRRGPNVLRVADLELDLL 98
L+E + + R RR + + + L+
Sbjct: 107 DLTE----LIGIIGRALAEPKRRPSKLEDDSQDGMPLV 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4612ECOLNEIPORIN815e-19 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 80.6 bits (199), Expect = 5e-19
Identities = 78/390 (20%), Positives = 134/390 (34%), Gaps = 64/390 (16%)

Query: 1 MKKTILLAASAATFSAAAVHAETSVTLYGLIDTGIGYAKVDGSYTNPNTGAKADVNASRI 60
MKK+++ A T +A V A VTLYG I G+ T+ + AS
Sbjct: 1 MKKSLI----ALTLAALPVAAMADVTLYGTIKAGV--------ETSRSVAHNGAQAASVE 48

Query: 61 GATTGQTAGSRWGLRGKEDLGDGLYATFRLESGFDSTNGESSQGGRLFGREATVGLGSAD 120
T GS+ G +G+EDLG+GL A +++E +S G R + +GL
Sbjct: 49 TGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQ----SFIGL-KGG 103

Query: 121 WGEVRLGRQYNVASRMMGSLFGNQFGGFTQLTTGAGLGFSGSNWVRYDNL---ALYESPS 177
+G++R+GR +V + G + + Y+SP
Sbjct: 104 FGKLRVGRLNSVL----------KDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPE 153

Query: 178 FGGFRLSAGYSFNANDLSAAQSGFATADNTRAITSGLSYNNGPLLAFIAYEQLNASNKLS 237
F G S Y+ N N + N + + Y A+ + Q+ + +
Sbjct: 154 FAGLSGSVQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGG----AYKRHHQVQENVNIE 209

Query: 238 NAQTSATPRSFTVGAAYDFEVLKLIAAYERATDGWFAGKGLPSGANINGFQGTPSNAFVD 297
Q + A Y +A ++ ++ + + + A+
Sbjct: 210 KYQIHRLVSGYDNDALYAS-----VAVQQQDAKLVEENY-----SHNSQTEVAATLAYRF 259

Query: 298 GFSTN--SYLLGVAVPLGGASSMFGSWQRVDPNNSDLTGGDSTSNTFALGYSYKLSKRTN 355
G T SY G ++ + +G Y SKRT+
Sbjct: 260 GNVTPRVSYAHGFKGSFDAT------------------NYNNDYDQVVVGAEYDFSKRTS 301

Query: 356 IYAAGSYTKNFAFQSDAKATEAIIGLRHVF 385
+ + + +S +T +GLRH F
Sbjct: 302 ALVSAGWLQEGKGESKFVSTAGGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4615PF05616300.028 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 30.1 bits (67), Expect = 0.028
Identities = 24/86 (27%), Positives = 37/86 (43%), Gaps = 12/86 (13%)

Query: 574 GYADKPAARPDDWSGRALPPVLPVSSDTDTSGLNEDGGLQIRPE--LDMLPTAAPLELEP 631
G A+ P A+P LP V P + + NE+ G + PE D+ P A P +
Sbjct: 318 GSAEAPNAQP-------LPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANP---DT 367

Query: 632 DLASMEEDDGPPLPVEPDRRLQRSAR 657
D D P +P P+ R ++ +
Sbjct: 368 DGQPGTRPDSPAVPDRPNGRHRKERK 393


108Bpet4676Bpet4681N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4676-1152.182135IclR family transcriptional regulator
Bpet4677-1131.37794250S ribosomal protein L21
Bpet4678-1132.44520450S ribosomal protein L27
Bpet4679-1141.203022GTPase ObgE
Bpet4680-1130.859188gamma-glutamyl kinase
Bpet4681-2120.515225putative transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4676YERSINIAYOPE290.025 Yersinia virulence determinant YopE protein signature.
		>YERSINIAYOPE#Yersinia virulence determinant YopE protein signature.

Length = 219

Score = 28.5 bits (63), Expect = 0.025
Identities = 20/94 (21%), Positives = 36/94 (38%), Gaps = 12/94 (12%)

Query: 131 REDARRPVRLVSELGSRL--PAHACA--LGKALLAGLPEPVLA-----ETLPPVLVQVSE 181
R ++ + L S + RL AH+ + + G +PV+ +P
Sbjct: 46 RTESPQGSSLASRIIERLSSVAHSVIGFIQRMFSEGSHKPVVTPAPTPAQMPSPTSFSDS 105

Query: 182 RTQTDRAAL---LRELAQARAEGLAQEHEEVAAG 212
Q L +++L AE L + H++ A G
Sbjct: 106 IKQLAAETLPKYMQQLNSLDAEMLQKNHDQFATG 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4678TYPE3IMRPROT250.025 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 25.5 bits (56), Expect = 0.025
Identities = 9/31 (29%), Positives = 12/31 (38%)

Query: 33 AGSIIVRQRGTRFHPGTNVGIGKDHTLFALI 63
AG II Q G F + + + A I
Sbjct: 98 AGEIIGLQMGLSFATFVDPASHLNMPVLARI 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4680CARBMTKINASE376e-05 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 37.5 bits (87), Expect = 6e-05
Identities = 27/106 (25%), Positives = 39/106 (36%), Gaps = 7/106 (6%)

Query: 138 GVVPIVNENDTVVTDEIRLGDNDTLGALVTNLIEADALVILTDQRGLYDSDPRKNPDAVF 197
G VP++ E+ + E + D D G + + AD +ILTD G + +
Sbjct: 195 GGVPVILEDGEIKGVEAVI-DKDLAGEKLAEEVNADIFMILTDVNGAALYYGTEKEQWLR 253

Query: 198 VAHAQAGDPELEAMAGGAGSGIGTGGMLTKILAAKRAAHSGAHTVI 243
+ E AGS M K+LAA R G I
Sbjct: 254 EVKVEELRKYYEEGHFKAGS------MGPKVLAAIRFIEWGGERAI 293


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4681HTHFIS300.008 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.2 bits (68), Expect = 0.008
Identities = 32/141 (22%), Positives = 53/141 (37%), Gaps = 4/141 (2%)

Query: 1 MQAAIEAIVIQGGVPRRPHLSSALAAMGWSVRECSAVGGLGNLVDARPPQVMMLEGPAQM 60
M A +V R L+ AL+ G+ VR S L + A +++ +
Sbjct: 1 MTGAT-ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPD 59

Query: 61 LCTLAGIARFTA--PRAALIVLADDTELEARVLALGAGADVACPLQIDLRE-LAALGRAL 117
+ R P ++V++ + A GA P DL E + +GRAL
Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 118 AQPRRDDVRPAAPVTPGWHLI 138
A+P+R + G L+
Sbjct: 120 AEPKRRPSKLEDDSQDGMPLV 140


109Bpet4954Bpet4961N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4954419-4.021962elongation factor Tu
Bpet4955017-3.236998elongation factor G
Bpet4956-215-1.19172130S ribosomal protein S7
Bpet4957-220-0.46165930S ribosomal protein S12
Bpet4958-120-0.384677two-component response regulator
Bpet4959-112-1.277881two-component response regulator
Bpet4960212-1.625989putative peptidoglycan binding protein
Bpet4961214-1.950385sensory box histidine kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4954TCRTETOQM857e-20 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 84.9 bits (210), Expect = 7e-20
Identities = 53/149 (35%), Positives = 81/149 (54%), Gaps = 5/149 (3%)

Query: 13 VNVGTIGHVDHGKTTLTAAI--TTVLSTKFGGEARGYDQIDAAPEEKARGITINTAHVEY 70
+N+G + HVD GKTTLT ++ + T+ G +G + D E+ RGITI T +
Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSF 63

Query: 71 ETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPY 130
+ E+ +D PGH D++ + + +DGAIL++SA DG QTR R++G+P
Sbjct: 64 QWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP- 122

Query: 131 IIVFLNKADMVDDAELLELVEMEVRELLS 159
I F+NK D L V +++E LS
Sbjct: 123 TIFFINKIDQN--GIDLSTVYQDIKEKLS 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4955TCRTETOQM6270.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 627 bits (1618), Expect = 0.0
Identities = 172/694 (24%), Positives = 297/694 (42%), Gaps = 77/694 (11%)

Query: 9 RYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAAT 68
+ NIG+ AH+DAGKTT TE +L+ +G ++G V G D E++RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 69 TAFWRGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWR 128
+ W ++NIIDTPGH+DF EV RS+ VLDGA ++ A GVQ Q+ ++
Sbjct: 62 SFQWEN-------TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFH 114

Query: 129 QANKYGVPRLAFVNKMDRTGANFFKVYDQLKTRLRANPVPIVIPIGAEDSFKGVIDLVKM 188
K G+P + F+NK+D+ G + VY +K +L A V + V M
Sbjct: 115 ALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIK----------QKVELYPNM 164

Query: 189 KAIIWDEASQGTKFDYVDIPAELEGAANEWREKLVEAAAESTEELMNKYLETGSLDEAEI 248
+ E+ Q + E ++L+ KY+ SL+ E+
Sbjct: 165 CVTNFTESEQ------------------------WDTVIEGNDDLLEKYMSGKSLEALEL 200

Query: 249 NTAIRQRTIAGEIQPMLCGTAFKNKGVQRMLDAVIDYLPSPIDIPPVAGQDDEGNEISRK 308
R + P+ G+A N G+ +++ + + S
Sbjct: 201 EQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH----------------- 243

Query: 309 ADDGEKMSALAFKLMSDPFVGQLTFVRVYSGILKSGDTVYNPIKGKKERVGRLLQMHANN 368
++ FK+ +L ++R+YSG+L D+V K K ++ +
Sbjct: 244 -RGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYTSINGE 301

Query: 369 REEIKEVLAGDIAAVVG----LKDVTTGETLCDVDSHILLERMEFPEPVISQAVEPKSKA 424
+I + +G+I + L V G+T ER+E P P++ VEP
Sbjct: 302 LCKIDKAYSGEIVILQNEFLKLNSV-LGDTKLLPQR----ERIENPLPLLQTTVEPSKPQ 356

Query: 425 DQEKMGLALSRLAQEDPSFRVRSDEESGQTIISGMGELHLEILVDRMKREFGVEANVGKP 484
+E + AL ++ DP R D + + I+S +G++ +E+ ++ ++ VE + +P
Sbjct: 357 QREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEP 416

Query: 485 QVAYRETIRKTCEEVEGKFVKQSGGRGQYGHVVLKLEPLEPGGGYEFVDAIKGGVVPREY 544
V Y E K E + + + L + PL G G ++ ++ G + + +
Sbjct: 417 TVIYMERPLKK---AEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF 473

Query: 545 IPAVDKGIQETLPAGILAGYPVVDVKATLFFGSYHDVDSNENAFKMAASMAFKEGMRKAS 604
AV +GI+ G L G+ V D K +G Y+ S F+M A + ++ ++KA
Sbjct: 474 QNAVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAG 532

Query: 605 PVLLEPMMAVEVETPEDYAGTVMGDLSSRRGMVQGMEDMVGGGKTIKAEVPLAEMFGYAT 664
LLEP ++ ++ P++Y D + + + + E+P + Y +
Sbjct: 533 TELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCIQEYRS 591

Query: 665 NLRSLTQGRATYTMEFKHYSEAPKNVADEVIAAR 698
+L T GR+ E K Y + V R
Sbjct: 592 DLTFFTNGRSVCLTELKGYHVT---TGEPVCQPR 622


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4958HTHFIS465e-08 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 46.0 bits (109), Expect = 5e-08
Identities = 30/138 (21%), Positives = 53/138 (38%), Gaps = 5/138 (3%)

Query: 1 MKQLKVLIMVPDATVRGHSVMTMLQAGIAAEACGTPLELFKLLGEDQYDAVVLDIGELGE 60
M +L+ DA +R + +AG L++ + D VV D+ E
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 IGYALLSRLRA-SPVLGIVVIGAGLGIENRLRCLQSGADICLPDPVDSRELACVLLALAR 119
+ LL R++ P L ++V+ A ++ + GA LP P D E L+ +
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTE----LIGIIG 116

Query: 120 RLPGRASEEIAQAESAPE 137
R ++ E +
Sbjct: 117 RALAEPKRRPSKLEDDSQ 134


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4959HTHFIS721e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 71.8 bits (176), Expect = 1e-16
Identities = 28/131 (21%), Positives = 55/131 (41%), Gaps = 1/131 (0%)

Query: 7 EDDLDQARHIKQVLDSAGYTCHSYQRSRDLLAAVRNQSFDLIMLDWQLPDMDGDEVLRRL 66
+DD + Q L AGY + L + DL++ D +PD + ++L R+
Sbjct: 10 DDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRI 69

Query: 67 RSTLGMQVPIIFLTSRSQEIDLVQGLHAGADDYVVKPLRSAELLARIAALLRRSQAAQPD 126
+ +P++ +++++ + ++ GA DY+ KP EL+ I L +
Sbjct: 70 KK-ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSK 128

Query: 127 HAPFSVAGYDI 137
S G +
Sbjct: 129 LEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4961PF06580350.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 34.8 bits (80), Expect = 0.001
Identities = 21/105 (20%), Positives = 39/105 (37%), Gaps = 26/105 (24%)

Query: 667 LLDNAIKY----SPDGSRIQLRVRAAAEMWDVEIEDAGPGIAPQDQQRLFQPFFRTGEAR 722
L++N IK+ P G +I L+ +E+E+ G ++
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE------------- 309

Query: 723 RSSTGGAGLGLAFVRA-VALRHGGE--ITVHSERGSGTRFTLRLP 764
G GL VR + + +G E I + ++G + +P
Sbjct: 310 -----STGTGLQNVRERLQMLYGTEAQIKLSEKQG-KVNAMVLIP 348


110Bpet4983Bpet4988N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Bpet4983-213-2.072708sensor histidine kinase TctE
Bpet4984-18-2.122917response regulator protein
Bpet4985010-2.134136O6-methylguanine-DNA methyltransferase
Bpet4986011-2.120649hypothetical protein
Bpet4987-112-2.121144putative nitrite extrusion protein
Bpet4988-111-1.767483nitrite extrusion protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4983PF06580424e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 42.2 bits (99), Expect = 4e-06
Identities = 55/316 (17%), Positives = 107/316 (33%), Gaps = 72/316 (22%)

Query: 192 GRVIVQVAETLESRQDFLQRALVRAVERDIVVIAIVVLVVVWSVFMAVRPLERLRQEVEG 251
G V+ + RQ +L+ + + + V+ A VV+ +VW V + RL +
Sbjct: 52 GLVLTHAYRSFIKRQGWLKLNMGQII--LRVLPACVVIGMVWFVANTS--IWRLLAFINT 107

Query: 252 RSADDLSPVDATNIPGEVGPL----VDAVNLHMARFAAQARLQRQFLDDASHQLRTPLSV 307
+ P+ + I V + H + QA + + + + + + L
Sbjct: 108 KPVAFTLPLALSIIFNVVVVTFMWSLLYFGWHFFKNYKQAEIDQWKMASMAQEAQ--LMA 165

Query: 308 LRTQ-----------TAYALRESDPQEVRAVLLAMQEGLDRAVRTTNQMLALARAKDAPL 356
L+ Q AL DP + R +L ++ E + R + L + A+ L
Sbjct: 166 LKAQINPHFMFNALNNIRALILEDPTKAREMLTSLSELM----RYS---LRYSNARQVSL 218

Query: 357 SEDGLAPERVDLAEVAQGVIRALLPAARAR-----QIDLGLEASSLPVCIPGVDWLLREA 411
++ E+ + + L A + Q + + + + V +P
Sbjct: 219 AD-----------ELTV--VDSYLQLASIQFEDRLQFENQINPAIMDVQVP------PML 259

Query: 412 LSNLVDNAIRY----TAPASQVTVRVYADERYARLTVEDNGPGMSAEDIARASVRFRRGA 467
+ LV+N I++ ++ ++ D L VE+ G
Sbjct: 260 VQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLA---------------- 303

Query: 468 AGKNKPGAGLGLAIVR 483
K G GL VR
Sbjct: 304 LKNTKESTGTGLQNVR 319


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4984HTHFIS903e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 90.3 bits (224), Expect = 3e-23
Identities = 41/161 (25%), Positives = 75/161 (46%), Gaps = 4/161 (2%)

Query: 2 RILLIEDEAELARWLSRSLARHAGFVVEWADDGLLAERRLAVEEFDAVILDLGLPGMDGH 61
IL+ +D+A + L+++L+R AG+ V + R +A + D V+ D+ +P +
Sbjct: 5 TILVADDDAAIRTVLNQALSR-AGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 TLLSKIRARDDRTPVLVLTARDSLAERVGTLHEGADDFLPKPFVLEE-LEARLTALIRRS 120
LL +I+ PVLV++A+++ + +GA D+LPKPF L E + AL
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 121 RGREHPRLAL--GDLILDTSAQRFTVRGQPLQLSPREHAVL 159
R G ++ SA + +L + ++
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLM 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4987TCRTETA432e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 42.9 bits (101), Expect = 2e-06
Identities = 70/353 (19%), Positives = 116/353 (32%), Gaps = 52/353 (14%)

Query: 56 TEFGLLAATPVLSGSLIRVPLGIWTDRYGGRIVFFLLMLVSVPGVWLLAYATEYWQFLVL 115
+G+L A L LG +DR+G R V + + + ++A A W +
Sbjct: 43 AHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG 102

Query: 116 GLFIGLAGGSFSVGTPYVA---------RWFPRNRQGLAMGIFGAGNSGSALTKFVA-PA 165
+ G+ G + +V Y+A R F G FG G VA P
Sbjct: 103 RIVAGITGATGAVAGAYIADITDGDERARHF-----GFMSACFGFG--------MVAGPV 149

Query: 166 LIAAAGGAWVIVPQVYAVALLATAILFWMF-------SATNPAHNVRGGASLSAQLAMLR 218
L GG P A AL L F P S + A
Sbjct: 150 LGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGM 209

Query: 219 DPRVWRYCQYYSVVFGGYVALALWMTKYYVGEYGFDMKLAALLAACFSLPGGVLRA-VGG 277
++ + G V ALW+ + + +D + A F + + +A + G
Sbjct: 210 TVVAALMAVFFIMQLVGQVPAALWVI-FGEDRFHWDATTIGISLAAFGILHSLAQAMITG 268

Query: 278 WISDKYGAYRTTWWVMWVCWVAFFLLSYPQTHFVIETDTGPREFFLAIGPTLFTILMFVV 337
++ + G R M + LL+ F G F I++ +
Sbjct: 269 PVAARLGERRALMLGMIADGTGYILLA-----------------FATRGWMAFPIMVLLA 311

Query: 338 GIAMAVGKASVFKFISDEFG-ENIGAVSGIVGLAGGLGGFVLPILFGALLDFT 389
+ G ++ +S + E G + G + L V P+LF A+ +
Sbjct: 312 SGGI--GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAAS 362


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Bpet4988TCRTETA330.003 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.9 bits (75), Expect = 0.003
Identities = 23/105 (21%), Positives = 37/105 (35%), Gaps = 3/105 (2%)

Query: 67 WLTALPALSGATLRIFYSFLVPVFGGR---RWTAISTATLLIPAIGMGFALQDPTTSYPT 123
W +S A I +S + G R L + A G G+ L T
Sbjct: 243 WDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWM 302

Query: 124 LLALALLCGFGGGNFSSSMANISFFFPKEKKGFATGMNAGIGNLG 168
+ +L GG + A +S +E++G G A + +L
Sbjct: 303 AFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT 347



 
Contact Sachin Pundhir for Bugs/Comments.
For best view 1024 x 768 resolution & IE 6.0 or above recommended.