PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
GenomeNC_016845.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_016845 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1KPHS_53160KPHS_53080Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_53160123-3.838986tRNA uridine 5-carboxymethylaminomethyl
KPHS_53150326-6.023934glucose-inhibited division protein B
KPHS_53140438-5.420346ATP synthase subunit I
KPHS_53130336-5.107341ATP synthase F0 subunit A
KPHS_53120443-4.282780F0F1 ATP synthase subunit C
KPHS_53110441-4.312921ATP synthase F0 subunit B
KPHS_53100439-4.250481F0F1 ATP synthase subunit delta
KPHS_53090542-4.044803F0F1 ATP synthase subunit alpha
KPHS_53080434-4.882382F0F1 ATP synthase subunit gamma
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_53110TRNSINTIMINR290.009 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 28.9 bits (64), Expect = 0.009
Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 3/41 (7%)

Query: 84 SQILDEAKAEAEQERTKIV---AQAQAEIDAERKRAREELR 121
QI +AK E R + V AQAQ + + R +EEL+
Sbjct: 319 EQIAQQAKEAGEVARQQAVESNAQAQQRYEDQHARRQEELQ 359


2KPHS_52990KPHS_52680Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_52990-314-4.168383phosphate transporter permease subunit
KPHS_52980-213-4.575518phosphate transporter permease subunit
KPHS_52970-111-5.117215phosphate transporter subunit
KPHS_52960015-4.957746transcriptional regulator PhoU
KPHS_52950115-4.788846hypothetical protein
KPHS_52940216-4.990966carbohydrate-specific outer membrane porin
KPHS_52930-213-2.186773putative xylanase
KPHS_52920-214-2.185339putative xylanase
KPHS_52910-214-1.833982hypothetical protein
KPHS_52900-112-0.9605036-phosphogluconate phosphatase
KPHS_52890010-0.149667hypothetical protein
KPHS_528800110.969978putative membrane / transport protein
KPHS_528702121.909392putative oxidoreductase, flavoprotein
KPHS_528600131.148332hypothetical protein
KPHS_52850-1131.187592LysR family transcriptional regulator
KPHS_52840-1141.081149putative membrane transport protein
KPHS_52830-1160.331905hypothetical protein
KPHS_52820-117-0.207222tRNA modification GTPase
KPHS_52810017-1.414044putative inner membrane protein translocase
KPHS_52800219-1.211572ribonuclease P protein component
KPHS_52790118-1.54788650S ribosomal protein L34
KPHS_52780016-1.696671chromosomal replication initiator protein DnaA
KPHS_52770-113-1.503458DNA polymerase III subunit beta
KPHS_52760-111-1.658710recombination protein F
KPHS_52750-211-1.600277DNA gyrase subunit B
KPHS_52740015-1.186388sugar phosphatase
KPHS_52730216-1.492912hypothetical protein
KPHS_52720213-1.483070putative outer membrane protein
KPHS_52710215-0.960178galactonate utilization transcriptional
KPHS_52700314-0.7843082-oxo-3-deoxygalactonate kinase
KPHS_52690312-1.036298putative keto-hydroxyglutarate-aldolase/keto-
KPHS_52680312-1.582302putative mandelate racemase/muconate lactonizing
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52930OUTRMMBRANEA300.001 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 29.9 bits (67), Expect = 0.001
Identities = 14/23 (60%), Positives = 16/23 (69%)

Query: 1 MKRHAIYFALALAGAAFTLQAAP 23
MK+ AI A+ALAG A QAAP
Sbjct: 1 MKKTAIAIAVALAGFATVAQAAP 23


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52880TYPE3IMSPROT310.013 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 30.5 bits (69), Expect = 0.013
Identities = 22/114 (19%), Positives = 43/114 (37%), Gaps = 3/114 (2%)

Query: 331 LTAVVVGILFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVKWSDLTEAVPA--- 387
L+ VV +L PL + A A ++ G L++ + + A
Sbjct: 72 LSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKKINPIEGAKRI 131

Query: 388 FITAVMMPFSFSITEGIALGFISYCVMKIGTGRLRELSPCVIIVSLLFVLKIVF 441
F ++ F SI + + L + + ++K L +L C I + +I+
Sbjct: 132 FSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQILR 185


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52840TCRTETA582e-11 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 58.3 bits (141), Expect = 2e-11
Identities = 66/311 (21%), Positives = 118/311 (37%), Gaps = 14/311 (4%)

Query: 5 LLCSFALVLLYPSGIDMYLVGLPRIAQDLGASEAQLHIAFSVYLAGMASAML----FAGR 60
L+ + V L GI + + LP + +DL S + + + LA A G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSN-DVTAHYGILLALYALMQFACAPVLGA 65

Query: 61 IADRSGRKPVAIVGAAIFVIASLLCAQAHTSSHFLIGRFIQGIGAGSCYVVAFAILRDTL 120
++DR GR+PV +V A + + A A IGR + GI G+ VA A + D
Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADIT 124

Query: 121 DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKYPWQSLFYTMTGMGVMVAVLSVFILRE 180
D RA+ ++ V PVLG L M + + F+ + + + F+L E
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGL-MGGFSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 181 TRPTAPPQAASPQHDAGESLLNRFFLSRLLITTLSVTVILTYVNVSPVLMMEEMGFDRGT 240
+ + S + ++ ++V I+ V P + G DR
Sbjct: 184 SHKGERRPLRREALNPLASFRWARGM-TVVAALMAVFFIMQLVGQVPAALWVIFGEDRFH 242

Query: 241 YSMAM------ALMAMISMAVSFSTPFALSLFNPRTLMLTSQVLFLAAGVTLSLATRQAV 294
+ A + S+A + T + R ++ + + L+ ATR +
Sbjct: 243 WDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWM 302

Query: 295 TLIGLGMICAG 305
+ ++ +G
Sbjct: 303 AFPIMVLLASG 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52830SACTRNSFRASE383e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.0 bits (88), Expect = 3e-06
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 3/55 (5%)

Query: 69 IVDVAVDPAHQGKGLGRLVMEKLVAWLDANAFDGAYV-TLVADVP--ELYAKFGF 120
I D+AV ++ KG+G ++ K + W N F G + T ++ YAK F
Sbjct: 92 IEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_5281060KDINNERMP7870.0 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 787 bits (2034), Expect = 0.0
Identities = 461/532 (86%), Positives = 493/532 (92%), Gaps = 2/532 (0%)

Query: 1 MIWQAWEQDKNPQPQ-QQTTQTTTTAAGSAADQGVPASGQGKLITVKTDVLELTINTNGG 59
MIWQAWEQDKNPQPQ QQTTQTTTTAAGSAADQGVPASGQGKLI+VKTDVL+LTINT GG
Sbjct: 18 MIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKLISVKTDVLDLTINTRGG 77

Query: 60 DIEQALLLAYPKTLKSTEPFQLLETTPQFVYQAQSGLTGRDGPDNPANGPRPLYNVDKEA 119
D+EQALL AYPK L ST+PFQLLET+PQF+YQAQSGLTGRDGPDNPANGPRPLYNV+K+A
Sbjct: 78 DVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDA 137

Query: 120 FVLADGQDELVIPLTYTDKAGNVFTKTFTLKRGGYAVNVGYSVQNASEKPLEVSTFGQLK 179
+VLA+GQ+EL +P+TYTD AGN FTKTF LKRG YAVNV Y+VQNA EKPLE+S+FGQLK
Sbjct: 138 YVLAEGQNELQVPMTYTDAAGNTFTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLK 197

Query: 180 QTAALPTSRDTQTGGLSTMHTFRGAAFSTADSKYEKYKFDTILDNENLNVSTKNGWVAML 239
Q+ LP DT + + +HTFRGAA+ST D KYEKYKFDTI DNENLN+S+K GWVAML
Sbjct: 198 QSITLPPHLDTGSSNFA-LHTFRGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAML 256

Query: 240 QQYFTTAWVPRNNGTNNFYTANLGNGIVAIGYKSQPVLVQPGQTDKLQSTLWVGPAIQDK 299
QQYF TAW+P N+GTNNFYTANLGNGI AIGYKSQPVLVQPGQT + STLWVGP IQDK
Sbjct: 257 QQYFATAWIPHNDGTNNFYTANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDK 316

Query: 300 MAAVAPHLDLTVDYGWLWFISQPLFKLLKFIHSFLGNWGFSIIVITFIVRGIMYPLTKAQ 359
MAAVAPHLDLTVDYGWLWFISQPLFKLLK+IHSF+GNWGFSII+ITFIVRGIMYPLTKAQ
Sbjct: 317 MAAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQ 376

Query: 360 YTSMAKMRMLQPKIQAMRERLGDDKQRQSQEMMALYKAEKVNPLGGCFPLIIQMPIFLAL 419
YTSMAKMRMLQPKIQAMRERLGDDKQR SQEMMALYKAEKVNPLGGCFPL+IQMPIFLAL
Sbjct: 377 YTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLAL 436

Query: 420 YYMLSASVELRHAPFILWIHDLSAQDPYYILPIIMGATMFFIQKMSPTTVTDPMQQKIMT 479
YYML SVELR APF LWIHDLSAQDPYYILPI+MG TMFFIQKMSPTTVTDPMQQKIMT
Sbjct: 437 YYMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMT 496

Query: 480 FMPVIFTVFFLWFPSGLVVYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS 531
FMPVIFTVFFLWFPSGLV+YYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS
Sbjct: 497 FMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS 548


3KPHS_52200KPHS_51920Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_52200-2143.584925putative LysR-family transcriptional regulator
KPHS_52190-2153.349374GlcNAc-PI de-N-acetylase
KPHS_52180-1164.039527hypothetical protein
KPHS_52170-1163.976452hypothetical protein
KPHS_521601183.839921putative dehydrogenase
KPHS_521501204.267642hypothetical protein
KPHS_52140-1224.914430inner membrane transporter yicL
KPHS_52130-1235.288110N-acetylmuramic acid phosphotransfer permease
KPHS_52120-1224.806824hypothetical protein
KPHS_52110-1214.482745putative general substrate transporter
KPHS_52100-2203.742880multidrug efflux transport outer membrane
KPHS_52090-2203.286118multidrug efflux permease EefB
KPHS_52080-1180.557979putative secretion protein
KPHS_520702190.702192hypothetical protein
KPHS_520602192.831771hypothetical protein
KPHS_520503193.592142putative acetyltransferase
KPHS_520402183.947597hypothetical protein
KPHS_520302183.737828putative TetR family transcriptional regulator
KPHS_520203184.598279multidrug resistance protein membrane
KPHS_520100173.777166putative LysR-family transcriptional regulator
KPHS_520001173.468562putative enzyme with a phophatase-like domain
KPHS_51990-1183.49240750S ribosomal protein L21
KPHS_51980-1173.538077hypothetical protein
KPHS_51970-1184.211028putative AraC-type regulatory protein
KPHS_51960-1163.282971hypothetical protein
KPHS_51950-1142.767089hypothetical protein
KPHS_51940-1152.612478hypothetical protein
KPHS_519300153.119208hypothetical protein
KPHS_51920-1143.044655AraC family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52110TCRTETA694e-15 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 69.5 bits (170), Expect = 4e-15
Identities = 80/339 (23%), Positives = 132/339 (38%), Gaps = 25/339 (7%)

Query: 27 LPALPEITQQLQATSTQTQLSLTAALIGLGLGQLFFGP----LSDRIGRLKPLALSLLLF 82
+P LP + + L S L L Q P LSDR GR L +SL
Sbjct: 25 MPVLPGLLRDLVH-SNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGA 83

Query: 83 IFSSAMCALTRDINMLIVWRFLQGFAGAGGSVLSRSIARDKYQGTLLTQFFALLMTVNGI 142
A+ A + +L + R + G GA G+V IA D G + F + G
Sbjct: 84 AVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA-DITDGDERARHFGFMSACFGF 142

Query: 143 APVLSPVLGGYVITAFDWRILFWTMAAIGGVLLVMSLAILRETRPATAAHASRQRPGQPV 202
V PVLGG + F F+ AA+ G+ + +L E+ R+
Sbjct: 143 GMVAGPVLGGL-MGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNP-- 199

Query: 203 LKNRRFLRFCLIQAFMMA-----GLFSYIGSSSFVMQSE--YGMSAMQFSLLFGLNGI-G 254
L + R+ R + A +MA L + ++ +V+ E + A + GI
Sbjct: 200 LASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILH 259

Query: 255 LIIAAMIFSRLARRFSAESLLRGGLTLAVSCAAIMLLFA---WLHLPVLALVGL--FFTV 309
+ AMI +A R L G+ +A I+L FA W+ P++ L+
Sbjct: 260 SLAQAMITGPVAARLGERRALMLGM-IADGTGYILLAFATRGWMAFPIMVLLASGGIGMP 318

Query: 310 SLMSGISTVAGAEAMSAVDAAQSG--TASALMGTLMFVF 346
+L + +S E + + + + ++++G L+F
Sbjct: 319 ALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTA 357


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52090ACRIFLAVINRP11450.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1145 bits (2963), Expect = 0.0
Identities = 583/1031 (56%), Positives = 754/1031 (73%), Gaps = 6/1031 (0%)

Query: 3 SRFFVRRPVFAWVIAILIMLAGVLAIRTLPVGQYPDVAPPAVKISATYTGASAETLENSV 62
+ FF+RRP+FAWV+AI++M+AG LAI LPV QYP +APPAV +SA Y GA A+T++++V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 63 TQVIEQQLTGLDHLLYFSSTSSSDGSVSITVTFEQGTDPDTAQVQVQNKVQQAESRLPSE 122
TQVIEQ + G+D+L+Y SSTS S GSV+IT+TF+ GTDPD AQVQVQNK+Q A LP E
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 123 VQQSGVTVEKSQSSFLLILAVYDKTNRATSSDISDWLVSNMQDPLARVEGVGSLQVFGAE 182
VQQ G++VEKS SS+L++ T DISD++ SN++D L+R+ GVG +Q+FGA+
Sbjct: 122 VQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ 181

Query: 183 YAMRVWMDPTKLASYSLMPSDVQSAIEAQNVQVSAGKIGALPSSNAQQLTATVRAQSRLQ 242
YAMR+W+D L Y L P DV + ++ QN Q++AG++G P+ QQL A++ AQ+R +
Sbjct: 182 YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 243 TPDQFKAIIVKSQADGSVVRLSDVARVEMGSEDYTATANLNGHPAAGIAVMMAPGANALD 302
P++F + ++ +DGSVVRL DVARVE+G E+Y A +NG PAAG+ + +A GANALD
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 303 TATLVKSKIAEFQRQMPQGYDIAYPKDSTEFIKISVEDVIQTLFEAIILVVCVMYLFLQN 362
TA +K+K+AE Q PQG + YP D+T F+++S+ +V++TLFEAI+LV VMYLFLQN
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 363 FRATLIPAVAVPVVLLGTFGVLALFGYSINTLTLFAMVLAIGLLVDDAIVVVENVERIMR 422
RATLIP +AVPVVLLGTF +LA FGYSINTLT+F MVLAIGLLVDDAIVVVENVER+M
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 423 DEGLPAREATEKSMGEISGALVAIALVLSAVFLPMAFFGGSTGVIYRQFSVTIISAMMLS 482
++ LP +EATEKSM +I GALV IA+VLSAVF+PMAFFGGSTG IYRQFS+TI+SAM LS
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 483 VVVALTLTPALCGALL----SHSKPHTKGFFGAFNRLWGRTEAGYQRRVLGGLRRGAVMM 538
V+VAL LTPALC LL + + GFFG FN + + Y V L +
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 539 GAYALICGAMALAMWKLPGSFLPVEDQGEIMVQYTLPAGATAVRTAEVRRQVTDWFLTKE 598
YALI M + +LP SFLP EDQG + LPAGAT RT +V QVTD++L E
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 599 KANTDVIFTVDGFSFSGSGQNAGMAFVSLKNWSQRKGDDNTAQAIALRATKELGTIRDAT 658
KAN + +FTV+GFSFSG QNAGMAFVSLK W +R GD+N+A+A+ RA ELG IRD
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 659 LFAMTPPSVDGLGQSNGFTFELMASGGTDRDSLMKLRSQLLAAANQS-SELQSVRANDLP 717
+ P++ LG + GF FEL+ G D+L + R+QLL A Q + L SVR N L
Sbjct: 662 VIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLE 721

Query: 718 QMPQLQVDIDNNKAVSLGLSLSDVTDTLSSAWGGTYVNDFIDRGRVKKVYIQGESDARAV 777
Q ++++D KA +LG+SLSD+ T+S+A GGTYVNDFIDRGRVKK+Y+Q ++ R +
Sbjct: 722 DTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRML 781

Query: 778 PSDLGKWFVRGSDNSMTPFSAFATTHWQYGPESLVRYNGSAAFEIQGENAAGFSSGAAMD 837
P D+ K +VR ++ M PFSAF T+HW YG L RYNG + EIQGE A G SSG AM
Sbjct: 782 PEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMA 841

Query: 838 KMEKLADSLPAGSTWAWSGISLQEKLASGQAMSLYAISILVVFLCLAALYESWSVPFSVI 897
ME LA LPAG + W+G+S QE+L+ QA +L AIS +VVFLCLAALYESWS+P SV+
Sbjct: 842 LMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVM 901

Query: 898 MVIPLGLLGAALAATLRGLSNDVYFQVALLTTIGLSSKNAILIVEFAESAVD-EGYSLSR 956
+V+PLG++G LAATL NDVYF V LLTTIGLS+KNAILIVEFA+ ++ EG +
Sbjct: 902 LVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVE 961

Query: 957 AAIRAAQTRLRPIVMTSLAFIAGVLPLAIATGAGANSRVAIGTGIIGGTLTATLLAVFFV 1016
A + A + RLRPI+MTSLAFI GVLPLAI+ GAG+ ++ A+G G++GG ++ATLLA+FFV
Sbjct: 962 ATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFV 1021

Query: 1017 PLFFVLVKRLF 1027
P+FFV+++R F
Sbjct: 1022 PVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52080RTXTOXIND422e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 42.1 bits (99), Expect = 2e-06
Identities = 24/133 (18%), Positives = 51/133 (38%), Gaps = 10/133 (7%)

Query: 8 PVPVVSQLTGRTTAS-LSAEVRPQVGGIIQKRLFTEGDMVKAGQALYQIDPSSYRATWNE 66
V +V+ G+ T S S E++P I+++ + EG+ V+ G L ++ A +
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 67 AAAALKQAQALVASDCQKAQRYASLVRDNGVSRQDADDAASTCAQDKASV--------ES 118
++L QA+ Q R L + + D + ++ + +
Sbjct: 139 TQSSLLQARLEQTRY-QILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFST 197

Query: 119 KKAALESARINLN 131
+ +NL+
Sbjct: 198 WQNQKYQKELNLD 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52050SACTRNSFRASE361e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.5 bits (84), Expect = 1e-05
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 6/72 (8%)

Query: 51 GLIAKRKGNW---LCIEYLWVSETTRGRGLGSELMQEAEQQAQAQGCSHLLVDTFSFQ-- 105
G I R NW IE + V++ R +G+G+ L+ +A + A+ L+++T
Sbjct: 78 GRIKIRS-NWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINIS 136

Query: 106 ALPFYQKLGYQL 117
A FY K + +
Sbjct: 137 ACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52030HTHTETR734e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 73.1 bits (179), Expect = 4e-18
Identities = 33/175 (18%), Positives = 70/175 (40%), Gaps = 10/175 (5%)

Query: 12 RPGRPRGKKPGTANREQLMDIALTLFARDGAGRVSLNAIAKEAGVTPAMLHYYFSSRDAL 71
R + ++ R+ ++D+AL LF++ G SL IAK AGVT ++++F + L
Sbjct: 3 RKTKQEAQE----TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDL 58

Query: 72 VTQLIEERFMPLRNHISRIFVDHPQDPVL----ALTMMVETLGHMAEKNAWFAPLWM-QE 126
+++ E + P DP+ L ++E+ + ++ E
Sbjct: 59 FSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCE 118

Query: 127 IIGEMPILRQHMDARFGEERFQVMLGTVRRWQQEGKINPALAPELLFTTVISLVL 181
+GEM +++Q E + + T++ + + L + +
Sbjct: 119 FVGEMAVVQQ-AQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYIS 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52020TCRTETB1384e-38 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 138 bits (350), Expect = 4e-38
Identities = 94/418 (22%), Positives = 178/418 (42%), Gaps = 19/418 (4%)

Query: 20 LLLVMLLSALDQTIVSTALPTIVGELGGL-DKLSWVVTAYILSSTIAVPLYGKFGDLFGR 78
L ++ S L++ +++ +LP I + +WV TA++L+ +I +YGK D G
Sbjct: 19 LCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGI 78

Query: 79 KIVLQVAIGLFLVGSALCGLAQNMTQLVLM-RGLQGLGGGGLMVISMAAVADVIPPANRG 137
K +L I + GS + + + L++M R +QG G + M VA IP NRG
Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138

Query: 138 RYQGLFGGVFGLATVIGPLIGGFLVQHASWRWIFYINLPLGLFALLVIGAVFHSSNKRSQ 197
+ GL G + + +GP IGG + + W ++ +P+ + R +
Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPMITIITVPFLMKLLKKEVRIK 196

Query: 198 HQIDWLGAIYLSMALLCIILFTSEGGSVHAWNDPQLWCILAFGIVGIIGFIYEERMAAEP 257
D G I +S+ ++ +LFT+ L ++ + F+ R +P
Sbjct: 197 GHFDIKGIILMSVGIVFFMLFTTS----------YSISFLIVSVLSFLIFVKHIRKVTDP 246

Query: 258 IIPLALFRNRSFLLCSLIGFVIGMSLFGSVTFLPLYLQVVKEATPTEAGLQLI-PLMGGL 316
+ L +N F++ L G +I ++ G V+ +P ++ V + + E G +I P +
Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSV 306

Query: 317 LLTSIISGRIISRTGKYRLFPILGTLLGVTGMVLLTRITIHSPLWQLYLFTGVLGAGLGL 376
++ I G ++ R G +G + + + + + + VLG GL
Sbjct: 307 IIFGYIGGILVDRRGP-LYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSF 364

Query: 377 VMQVLVLAVQNAMPAQMYGVATSGATLFRSIGGSIGVALFGAVFTHVLQSNLQQLLPE 434
V+ V +++ Q G S + G+A+ G + + + Q+LLP
Sbjct: 365 TKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS--IPLLDQRLLPM 420


4KPHS_51590KPHS_51480Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_51590221-2.604316putative voltage-gated ClC-type chloride channel
KPHS_51580237-10.012133putative methylated-DNA-[protein]-cysteine
KPHS_51570142-12.194915hypothetical protein
KPHS_51560345-13.307195hypothetical protein
KPHS_51550345-13.277591K+-transporting ATPase
KPHS_51540253-14.940632hypothetical protein
KPHS_51530151-14.113167putative acetyltransferase
KPHS_51520042-11.264776hypothetical protein
KPHS_51510-135-8.796227hypothetical protein
KPHS_51500-225-6.561558putative ABC-type multidrug transport system
KPHS_51490-222-5.378180putative transposase
KPHS_51480-121-4.562422putative ABC-type multidrug transport system,
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_51510RTXTOXIND581e-11 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 57.9 bits (140), Expect = 1e-11
Identities = 48/351 (13%), Positives = 121/351 (34%), Gaps = 85/351 (24%)

Query: 21 IERILINKGDNVAAGQELVKIESFDA-------QNIFLRAEEKLSAESALLRNLESGERP 73
++ I++ +G++V G L+K+ + A Q+ L+A + + L R++E + P
Sbjct: 107 VKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLP 166

Query: 74 E-----------------------------------------------ELDIIRSQIKKA 86
E E + ++I +
Sbjct: 167 ELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRY 226

Query: 87 QSAESQVKRQLGRYRNLYANHAISLAEWEDIRDELTQKGAQVEEL---INQLKARQLPAR 143
++ K +L + +L AI+ + ++ + ++ + Q+++ L A+
Sbjct: 227 ENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAK 286

Query: 144 Q--------------DEISKQRSMVAAAKLERDKALWDVQQTTIVSPVNAKVFDI-IYRA 188
+ D++ + + LE K Q + I +PV+ KV + ++
Sbjct: 287 EEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTE 346

Query: 189 GERPSAGKPIISLLPPEN-IKVRFFIPEAKLGKFKIGSKVKLICDG----CAEPIAGVIN 243
G + + ++ ++P ++ ++V + +G +G + + + G +
Sbjct: 347 GGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVK 406

Query: 244 YISPEA---EFTPPVIYSTKRREKLIFMAEAIPALQQAGRMKIGQPFDVEI 291
I+ +A + V E+ + + + G EI
Sbjct: 407 NINLDAIEDQRLGLVFNVIISIEE-----NCLSTGNKNIPLSSGMAVTAEI 452


5KPHS_50530KPHS_50230Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_50530-1123.168444hypothetical protein
KPHS_50520-1133.403306hypothetical protein
KPHS_50510-1143.259739putative ATPase involved in chromosome
KPHS_50500-1153.288572putative cellulose synthase
KPHS_504900154.001375cellulose synthase regulator protein
KPHS_504800132.667562putative cellulose synthase
KPHS_50470-2110.131509hypothetical protein
KPHS_50460-1120.563815endo-1,4-D-glucanase
KPHS_50450-2110.646780hypothetical protein
KPHS_50440-3131.251590hypothetical protein
KPHS_50430-3131.705984hypothetical protein
KPHS_50420-1163.984964hypothetical protein
KPHS_50410-1174.081927cell division protein
KPHS_504000153.905344cellulose synthase catalytic subunit
KPHS_503900153.741126putative cellulose synthase
KPHS_503800123.465529endo-1,4-D-glucanase
KPHS_503700133.420231cellulose synthase subunit BcsC
KPHS_503600100.819539hypothetical protein
KPHS_50350-2121.454687phosphodiesterase
KPHS_50340-3131.509641C4-dicarboxylate transporter DctA
KPHS_50330-2142.212234putative peptidase
KPHS_50320-1162.459750ketodeoxygluconokinase
KPHS_503100152.339292antibiotic biosynthesis monooxygenase
KPHS_50300-1163.336500hypothetical protein
KPHS_50290-1163.195540metabolite/H+ symporter, major facilitator
KPHS_502800154.200717hypothetical protein
KPHS_502700164.215866putative LysR-family transcriptional regulator
KPHS_50260-2163.778847oxidoreductase
KPHS_50250-2183.642832putative acetyltransferase
KPHS_50240-2183.691915cytoplasmic trehalase
KPHS_50230-2193.390564putative iron-containing alcohol dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50520IGASERPTASE320.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 31.6 bits (71), Expect = 0.002
Identities = 21/105 (20%), Positives = 33/105 (31%), Gaps = 7/105 (6%)

Query: 72 RQVEAAVAAAPTSDPVVSAASAA------PATSQPVVETAAAAPEPVRQEPAPTPAPSIP 125
V+ A +DP V+ T QP ET++ +PV + S+
Sbjct: 1137 ETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVV 1196

Query: 126 ASEPAMAPPPRMAPRPAPAAENYAHLFAAKSAEPVAKNKDQPLKS 170
+ P P P + N +S V N + S
Sbjct: 1197 EN-PENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTS 1240



Score = 27.7 bits (61), Expect = 0.033
Identities = 18/128 (14%), Positives = 39/128 (30%), Gaps = 8/128 (6%)

Query: 43 NPGAEKSSSLALGGSVSAPLPQSVPADLFRQVEAAVAAAPTSDPVVSAASAAPATSQPVV 102
N + + + A + ++ +A V T + + +P Q
Sbjct: 1079 NTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSET 1138

Query: 103 ETAAAAPEPVRQEPAPTPAPSIPASEPAMAPPPRMAPRPAPAAENYAHLFAAKSAEPVAK 162
A EPA P++ EP + A PA E +++ +
Sbjct: 1139 VQPQA-------EPARENDPTVNIKEPQ-SQTNTTADTEQPAKETSSNVEQPVTESTTVN 1190

Query: 163 NKDQPLKS 170
+ +++
Sbjct: 1191 TGNSVVEN 1198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50480SYCDCHAPRONE300.035 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 30.3 bits (68), Expect = 0.035
Identities = 13/60 (21%), Positives = 21/60 (35%)

Query: 277 GYANLNSGNTAAAKQQFEEVLQTNPQDADALAGMGYIAQRSGDYQAASQYLSRAADLGGD 336
+ SG A + F+ + + D+ G+G Q G Y A S A +
Sbjct: 43 AFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIK 102


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50450FLGMRINGFLIF290.045 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 29.2 bits (65), Expect = 0.045
Identities = 8/44 (18%), Positives = 17/44 (38%), Gaps = 5/44 (11%)

Query: 93 QGSQIAGFSASYIWDLIVRFINWSMVGAFFVLLVLWLFISQWLR 136
G ++ + D ++ W VL+V W+ + +R
Sbjct: 442 TGGELPFWQQQSFIDQLLAAGRW-----LLVLVVAWILWRKAVR 480


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50310FLGMOTORFLIG270.004 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 27.1 bits (60), Expect = 0.004
Identities = 10/47 (21%), Positives = 19/47 (40%), Gaps = 5/47 (10%)

Query: 1 MHHVSPEYSQKAAFILEMRFSSHYARQVKK-----IMERCFNMIDQQ 42
M SPE ++ +LE + +S + + NM D++
Sbjct: 174 MDRTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRK 220


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50290TCRTETB310.008 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.4 bits (71), Expect = 0.008
Identities = 79/365 (21%), Positives = 126/365 (34%), Gaps = 59/365 (16%)

Query: 79 IGSALFGHFGDRVGRKVTLVASLLTMGISTVVIGLLPGYEIIGIVAPMLLALARFGQGLG 138
IG+A++G D++G K LL GI G + G+ +G LL +ARF QG G
Sbjct: 64 IGTAVYGKLSDQLGIK-----RLLLFGIIINCFGSVIGF--VGHSFFSLLIMARFIQGAG 116

Query: 139 LGGEWGGAALLATENAPARKR----ALYGSFPQLGAPIGFFFANGTFLLLSW-------- 186
++ P R L GS +G +G + W
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM 176

Query: 187 -----LLTDQQFMEWGWRV--PF-IFSAVLVIIG-------------LYVRVSLHETPVF 225
+ + ++ R+ F I +L+ +G ++ VS+ +F
Sbjct: 177 ITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIF 236

Query: 226 AKVAAAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFSTGAAPNGLGL 285
K + G + VL I+ T F M Y M + +G
Sbjct: 237 VKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIG- 295

Query: 286 PRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMII-ITTMIILFALFAFKPLLGSGN 344
+ +++ M+VI FG + G+L D G + I +T + + F +F S
Sbjct: 296 --SVIIFPGTMSVIIFG---YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWF 350

Query: 345 PLLVFAFLLLGLSLMGL---TFGPMGALLPELFPTEVRYTGASFS-YNVSSILGASVAPY 400
++ F+L GLS T L E GA S N +S L
Sbjct: 351 MTIIIVFVLGGLSFTKTVISTIVSSS-----LKQQEA---GAGMSLLNFTSFLSEGTGIA 402

Query: 401 IAAWL 405
I L
Sbjct: 403 IVGGL 407



Score = 29.5 bits (66), Expect = 0.026
Identities = 19/101 (18%), Positives = 38/101 (37%), Gaps = 2/101 (1%)

Query: 255 FIMLATYTLFYIMTVYSMTFSTGAAPNGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADA 314
I L + F ++ + S N P W+ ++ F + V G L+D
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 315 FGRRKSMIIITTMIILFALFAFKPLLGSGNPLLVFAFLLLG 355
G ++ ++ + ++ F + S LL+ A + G
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGF--VGHSFFSLLIMARFIQG 114


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50280DPTHRIATOXIN300.015 Diphtheria toxin signature.
		>DPTHRIATOXIN#Diphtheria toxin signature.

Length = 567

Score = 30.1 bits (67), Expect = 0.015
Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 6/40 (15%)

Query: 19 EKSKSTLEALNDTAVGQKASQALKTVTGTAAKVQRNPVIA 58
EK+K LE + TA+ LKTVTGT NPV A
Sbjct: 273 EKAKQYLEEFHQTALEHPELSELKTVTGT------NPVFA 306


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50260DHBDHDRGNASE857e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 85.1 bits (210), Expect = 7e-22
Identities = 67/258 (25%), Positives = 113/258 (43%), Gaps = 16/258 (6%)

Query: 4 RIALVTGGSRGLGKNAALKLAAKGTDILLTYHSNRQAALDVVAEIEQKGVKAAALALNVG 63
+IA +TG ++G+G+ A LA++G I N + VV+ ++ + A A +V
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 64 DSTTFDAFASEVAQVLAQKWGRTTFDYLLNNAGIGLNAPFAETSEAQFDELMNIQFKGPF 123
DS D E+ + ++ G D L+N AG+ S+ +++ ++ G F
Sbjct: 68 DSAAID----EITARIEREMGP--IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVF 121

Query: 124 FLTQRLLPLLQD--GGRILNVSSGLARFALPGYAAYAAMKGAMEVLTRYQAKELGGRGIS 181
++ + + D G I+ V S A AAYA+ K A + T+ EL I
Sbjct: 122 NASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181

Query: 182 VNIIAPGAIETDFGGG-EVRDNAE--VNRHIAAQTALG----RVGLPDDIGDAIAALLSD 234
NI++PG+ ETD +N V + G ++ P DI DA+ L+S
Sbjct: 182 CNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 235 ELAWMNAQRVEVSGGMFL 252
+ + + V GG L
Sbjct: 242 QAGHITMHNLCVDGGATL 259


6KPHS_50120KPHS_49850Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_501202153.951837putative oxidoreductase
KPHS_501106154.559054magnesium transporter
KPHS_501004165.431660nickel responsive regulator
KPHS_500904155.934961nickel transporter ATP-binding protein
KPHS_500801164.694663nickel transporter ATP-binding protein
KPHS_500700153.952186nickel transport system permease component
KPHS_50060-1163.288215nickel transporter permease NikB
KPHS_50050-2152.442438nickel ABC transporter periplasmic
KPHS_50040-1162.203662putative LysR-family transcriptional regulator
KPHS_50030-2161.665019putative phenolic acid decarboxylase
KPHS_50020-1162.411324putative aminotransferase
KPHS_50010-1182.432574undecaprenyl phosphate
KPHS_500000162.588177bifunctional UDP-glucuronic acid
KPHS_499903143.053159hypothetical protein
KPHS_499804163.1359514-amino-4-deoxy-L-arabinose transferase
KPHS_499706193.114046hypothetical protein
KPHS_499604181.951689hypothetical protein
KPHS_499504161.838779hypothetical protein
KPHS_499401124.229327PerM family permease
KPHS_499301134.199142major facilitator family transporter
KPHS_499200133.740703hypothetical protein
KPHS_499100123.486977hypothetical protein
KPHS_499001144.551576cell developmental protein SirA
KPHS_498902123.396878heavy-metal transporting ATPase ZntA
KPHS_498803130.516160hypothetical protein
KPHS_498704130.722940putative receptor
KPHS_498603130.277794hypothetical protein
KPHS_498502111.350739RsmD family RNA methyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50010ACRIFLAVINRP310.011 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 30.6 bits (69), Expect = 0.011
Identities = 20/97 (20%), Positives = 35/97 (36%), Gaps = 5/97 (5%)

Query: 182 TFIPILANTFARRAVEIPVMHAEREFGDSKYSFMRLINLMYDLVTCLTTTPLRLLSIFGS 241
P L T + + FG +F +N + V + + R L I+
Sbjct: 487 ILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYAL 546

Query: 242 VIALLGFAFGLLLVVLRLAFGPQWAAEGVFMLFAVLF 278
++A + F L +F P+ +GVF+ L
Sbjct: 547 IVAGMVVLFLRLPS----SFLPE-EDQGVFLTMIQLP 578


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50000NUCEPIMERASE1085e-28 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 108 bits (272), Expect = 5e-28
Identities = 73/361 (20%), Positives = 137/361 (37%), Gaps = 60/361 (16%)

Query: 317 RVLILGVNGFIGNHLTERLLQDDNYEIYGLDIGSD--------AISRFLDCPRFHFVEGD 368
+ L+ G GFIG H+++RLL + +++ G+D +D A L P F F + D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLL-EAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 369 ISIHSEWIE--YHIKKCDVVLPLVAIATPIEYT-RNPLRVFELDFEENLKIIRDCVKYN- 424
++ E + + + V + Y+ NP + + L I+ C
Sbjct: 61 LADR-EGMTDLFASGHFERVFISPHRLA-VRYSLENPHAYADSNLTGFLNILEGCRHNKI 118

Query: 425 KRIIFPSTSEVYGMCTDKNFDEDSSNLVVGPINKQRWIYSVSKQLLDRVIWAYGDKNGLK 484
+ +++ S+S VYG+ F D S V P++ +Y+ +K+ + + Y GL
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDS--VDHPVS----LYAATKKANELMAHTYSHLYGLP 172

Query: 485 FTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIEGGKQKRCFTDISDGI 544
T R F GP A+ + ++EG I + GK KR FT I D
Sbjct: 173 ATGLRFFTVYGPWGR--------PDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIA 224

Query: 545 EALFRIIEN---------------KDGRCDGQIINIGNPDNEASIKELAEMLLACFERHP 589
EA+ R+ + ++ NIGN + + + L
Sbjct: 225 EAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVE-LMDYIQALEDALGIEA 283

Query: 590 LRDRFPPFAGFREVESSDYYGKGYQDVEHRKPSIRNAKRCLNWEPKVEMEETVEHTLDFF 649
++ P G DV + + + P+ +++ V++ ++++
Sbjct: 284 KKNMLPLQPG---------------DVLETSADTKALYEVIGFTPETTVKDGVKNFVNWY 328

Query: 650 L 650

Sbjct: 329 R 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49970BCTERIALGSPC322e-04 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 32.2 bits (73), Expect = 2e-04
Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 1/32 (3%)

Query: 35 RHILFWLGMALLCLGCGMLLW-LSVLQSIPVS 65
R ILF+L M L C M+ W + + + PVS
Sbjct: 15 RRILFYLLMLLFCQQLAMIFWRIGLPDNAPVS 46


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49930TCRTETA471e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 46.7 bits (111), Expect = 1e-07
Identities = 74/365 (20%), Positives = 133/365 (36%), Gaps = 30/365 (8%)

Query: 13 LRLNLRIVSVVIFNFASYLTIGLPLAVLPGYVHDVM--GFSAFWAGLVISLQYFATLLSR 70
++ N ++ ++ + IGL + VLPG + D++ G++++L
Sbjct: 1 MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACA 60

Query: 71 PHAGRYADLLGPKKIVVFGLGGCFLSGLSYLLAAWGSGWPLISLLLLCLGRVILGI-GQS 129
P G +D G + +++ L + + Y + A L +L +GR++ GI G +
Sbjct: 61 PVLGALSDRFGRRPVLLVSL---AGAAVDYAIMATAP-----FLWVLYIGRIVAGITGAT 112

Query: 130 FAGTGSTLWGVGVVGSLHIGRVISWNGIVTYGAMAMGAPLGVLC--YSHIGLSGLAGVIM 187
A G+ + + R + M G LG L +S A +
Sbjct: 113 GAVAGAYIADITDGDER--ARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALN 170

Query: 188 AVALVAILCALP-------RAAVKAAKGKAMSFR-AVLGRVWPYGMALA-LASAGFGVIA 238
+ + LP R + A SFR A V MA+ + V A
Sbjct: 171 GLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPA 230

Query: 239 TFITLFYDAK-GWDGAAFALTLFSCAFVGA---RLLFPNAINLLGGLNVAMLCFSVEAIG 294
+F + + WD ++L + + + ++ LG ML + G
Sbjct: 231 ALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTG 290

Query: 295 LLLVGFADTPMMAKIGTFLTGAGFSLVFPALGVVAVKAVPQHNQGSALATYTVFMDLSLG 354
+L+ FA MA L A + PAL + + V + QG + L+
Sbjct: 291 YILLAFATRGWMAFPIMVLL-ASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT-S 348

Query: 355 VSGPL 359
+ GPL
Sbjct: 349 IVGPL 353


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49900PF012061033e-33 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 103 bits (259), Expect = 3e-33
Identities = 27/71 (38%), Positives = 43/71 (60%)

Query: 9 DHTLDALGLRCPEPVMMVRKTVRTMPVGETLLIIADDPATTRDIPGFCRFMEHELVAQET 68
D +LDA GL CP P++ +KT+ TM GE L ++A DP + +D F + HEL+ Q+
Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64

Query: 69 EALPYRYLIRK 79
E Y + +++
Sbjct: 65 EDGTYHFRLKR 75


7KPHS_46840KPHS_46690Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_46840-120-3.015844putative tagatose 6-phosphate kinase 1
KPHS_46830023-3.639287PTS family enzyme IIA/FPr
KPHS_46820020-4.019484PTS family enzyme IIB'BC, fructose-specific
KPHS_46810016-2.7328146-phosphofructokinase
KPHS_46800015-2.844436tagatose-bisphosphate aldolase
KPHS_46790014-3.376473hypothetical protein
KPHS_46780-111-2.537355putative DEOR-type transcriptional regulator
KPHS_46770-112-2.154151putative kinase
KPHS_46760011-1.274770(D)-galactarate dehydrogenase
KPHS_46750-114-2.507127hypothetical protein
KPHS_46740-113-2.696412putative transport protein
KPHS_46730015-2.640399alpha-dehydro-beta-deoxy-D-glucarate aldolase
KPHS_46720118-3.309342tartronate semialdehyde reductase
KPHS_46710019-3.626609glycerate kinase
KPHS_46700119-4.488956hypothetical protein
KPHS_46690016-3.895181hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_46820BCTERIALGSPD300.026 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 29.9 bits (67), Expect = 0.026
Identities = 17/81 (20%), Positives = 31/81 (38%), Gaps = 6/81 (7%)

Query: 160 ADPNSPQYNVIAATLMKVGQQAFSIMVPVFTAYIAWSISGRPGMVAGFVGGLLANATGAG 219
D + + A Q + +P+ TA + + G V+ + L++
Sbjct: 357 QDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSF---- 412

Query: 220 FLGGIIAGFAAGYFMLLIRHL 240
GI AGF G + +L+ L
Sbjct: 413 --NGIAAGFYQGNWAMLLTAL 431


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_46740TCRTETA448e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 44.0 bits (104), Expect = 8e-07
Identities = 65/394 (16%), Positives = 120/394 (30%), Gaps = 57/394 (14%)

Query: 46 KELGLS---AVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYSYSLFFWSLFTFLQGF 102
++L S G + + + G L D+FG + V SL ++ +
Sbjct: 33 RDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMAT 92

Query: 103 IDVFPLAWAGVSMFFMRFMLGFSEAPSFPANARIVAAWFPAKER----GTASAIFNAAQY 158
+ + G R + G + A +A ER G SA F
Sbjct: 93 APFLWVLYIG------RIVAGITGAT-GAVAGAYIADITDGDERARHFGFMSACFGFG-- 143

Query: 159 FSLALFSPLLGWLTFALGWEHVF---TVMGIIGFVLTIIWVKFVHNPTDHPRMSAAELKY 215
+ P+LG L F + + F+ + H P
Sbjct: 144 ---MVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRP--------- 191

Query: 216 ISEGGAVVDMDHKKEATPAAGPKMDYIRQLLTNRMMLGVFFGQYFLNTITWFFLTWFPIY 275
+ + R + ++ VFF + + + I+
Sbjct: 192 ------------LRREALNPLASFRWARGMTVVAALMAVFFI---MQLVGQVPAALWVIF 236

Query: 276 LVQDKGMSILKVGFVASIPALFGFAGGVLGGLFSDYLIGRGCTLTFARKLPIVLGMLL-A 334
+G + A G+L L + G + ++LGM+
Sbjct: 237 GEDRFHWDATTIGISLA-------AFGILHSLAQAMITGP-VAARLGERRALMLGMIADG 288

Query: 335 SSIILCNYTASTPLVITLMA-LAFFGKGFGALGWPVISDVAPKEIVGLCGGVFNVFGNVA 393
+ IL + + +M LA G G AL ++S +E G G ++
Sbjct: 289 TGYILLAFATRGWMAFPIMVLLASGGIGMPALQ-AMLSRQVDEERQGQLQGSLAALTSLT 347

Query: 394 SIATPLVIGYIVSELHSFNGALIFVGGSALMMMV 427
SI PL+ I + + ++ G+AL ++
Sbjct: 348 SIVGPLLFTAIYAASITTWNGWAWIAGAALYLLC 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_46730PHPHTRNFRASE378e-05 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 37.1 bits (86), Expect = 8e-05
Identities = 16/82 (19%), Positives = 31/82 (37%), Gaps = 12/82 (14%)

Query: 144 KNISILVQIESQTGVDNVEAIAATEGVDGVFVGPSDLA----------AALGHLGNAAHP 193
+I + + +E + A + VD +G +DL + +L HP
Sbjct: 423 DSIEVGIMVEIPSTAVAANLFA--KEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHP 480

Query: 194 EVQRAIQHIFASAKKHGKPSGI 215
+ R + + +A GK G+
Sbjct: 481 AILRLVDMVIKAAHSEGKWVGM 502


8KPHS_46470KPHS_46300Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_464700133.402147hypothetical protein
KPHS_464600144.309234helix-turn-helix motif-containing protein
KPHS_464501134.169833hypothetical protein
KPHS_464400124.032015hypothetical protein
KPHS_464300134.6446832,4-dienoyl-CoA reductase
KPHS_46420-1134.213878putative kinase
KPHS_46410-1153.459535putative SorC-family transcriptional regulator
KPHS_46400-1162.691028ATPase
KPHS_463900162.052395putative ABC transporter permease
KPHS_46380-1181.569888putative transport system permease component
KPHS_463700170.248615autoinducer-2 ABC superfamily ATP binding
KPHS_46360-1170.709105aldolase
KPHS_46350-1152.401141lsrG protein
KPHS_463400133.254619putrescine aminotransferase
KPHS_463301153.764940hypothetical protein
KPHS_463200153.518478hypothetical protein
KPHS_463100163.555338putative enzyme, ferredoxin reductase-like,
KPHS_463000153.082975putative dihydroxyacetone kinase
9KPHS_46120KPHS_45860Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_461202140.263914YfdX like protein
KPHS_46110211-0.394810putative sensor protein EvgS1
KPHS_46100011-2.005790DNA-binding transcriptional activator EvgA
KPHS_46090114-1.562490hypothetical protein
KPHS_46080113-0.487431hypothetical protein
KPHS_46070-1140.028277*G/U mismatch-specific DNA glycosylase
KPHS_46060015-0.200488RNA polymerase sigma factor RpoD
KPHS_46050-2121.669679DNA primase
KPHS_46040-3163.10725430S ribosomal protein S21
KPHS_460300193.916016O-sialoglycoprotein endopeptidase
KPHS_460201203.869117permease, cytosine/purine, uracil, thiamine,
KPHS_460101214.543963urease accessory protein UreG
KPHS_460001205.129869urease accessory protein UreF
KPHS_459902194.115016urease accessory protein
KPHS_459802194.144581urease subunit alpha
KPHS_459701183.406220urease subunit beta
KPHS_459600181.608772urease subunit gamma
KPHS_45950-115-0.274637urease accessory protein UreD
KPHS_45940-1130.658838short-chain dehydrogenase
KPHS_45930-1120.321254hypothetical protein
KPHS_45920-2121.410428hypothetical protein
KPHS_459100122.773935dihydroneopterin aldolase
KPHS_459000133.293905undecaprenyl pyrophosphate phosphatase
KPHS_458900133.721761tRNA nucleotidyl transferase
KPHS_458801122.865274putative signal transduction protein
KPHS_458702133.253108hypothetical protein
KPHS_458602132.715280bifunctional glutamine-synthetase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_46110HTHFIS762e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.4 bits (188), Expect = 2e-16
Identities = 35/157 (22%), Positives = 59/157 (37%), Gaps = 1/157 (0%)

Query: 846 ILIADDHPTNRLLLKRQLSTIGYSVDEACDGEEAENKLASKHYDLLITDLNMPKKDGLAL 905
IL+ADD R +L + LS GY V + +A+ DL++TD+ MP ++ L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 906 AASLRRRYPGLVIWGVTASALPQSREACLASGMNMCLFKPVSVQTLSHELSRLAVGRASP 965
+++ P L + ++A + G L KP + + A+
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTE-LIGIIGRALAEPKR 124

Query: 966 HATRHLKLSVLSENTGGDQALMNEMLETFRDASAADL 1002
++ S G A M E+ DL
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDL 161


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_46100HTHFIS614e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 60.6 bits (147), Expect = 4e-13
Identities = 23/114 (20%), Positives = 53/114 (46%), Gaps = 1/114 (0%)

Query: 4 IIIDDHPLARIAIRNLLDSNGITVAAELDSGAHAVQTAESMQPDLLIVDVDIPELSGIEV 63
++ DD R + L G V + A + + DL++ DV +P+ + ++
Sbjct: 7 LVADDDAAIRTVLNQALSRAGYDVRIT-SNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 64 LEQLRKRRYQGTIIIISAKNELFYGKRSADCGANGFVSKKEGMNNILAAIDAAN 117
L +++K R ++++SA+N ++++ GA ++ K + ++ I A
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45980UREASE10850.0 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 1085 bits (2809), Expect = 0.0
Identities = 411/566 (72%), Positives = 474/566 (83%), Gaps = 2/566 (0%)

Query: 4 ISRQAYADMFGPTVGDKVRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGMGQGQML-A 62
+SR AYA+MFGPTVGDKVRLADTEL+IEVE D TT+GEEVKFGGGKVIRDGMGQ Q+
Sbjct: 5 MSRAAYANMFGPTVGDKVRLADTELFIEVEKDFTTHGEEVKFGGGKVIRDGMGQSQVTRE 64

Query: 63 ADCVDLVLTNALIVDHWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIGASTEVIAAE 122
VD V+TNALI+DHWGIVKADIG+KDGRI AIGKAGNPD+QP VTI +G TEVIA E
Sbjct: 65 GGAVDTVITNALILDHWGIVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGE 124

Query: 123 GKIVTAGGIDTHIHWICPQQAEEALVSGVTTMVGGGTGPAAGTHATTCTPGPWYISRMLQ 182
GKIVTAGG+D+HIH+ICPQQ EEAL+SG+T M+GGGTGPA GT ATTCTPGPW+I+RM++
Sbjct: 125 GKIVTAGGMDSHIHFICPQQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIARMIE 184

Query: 183 AADSLPVNIGLLGKGNVSQPDALREQVAAGVIGLKIHEDWGATPAAIDCALTVADEMDIQ 242
AAD+ P+N+ GKGN S P AL E V G LK+HEDWG TPAAIDC L+VADE D+Q
Sbjct: 185 AADAFPMNLAFAGKGNASLPGALVEMVLGGATSLKLHEDWGTTPAAIDCCLSVADEYDVQ 244

Query: 243 VALHSDTLNESGFVEDTLAAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNPTL 302
V +H+DTLNESGFVEDT+AAI GRTIH +HTEGAGGGHAPDII C PN++PSSTNPT
Sbjct: 245 VMIHTDTLNESGFVEDTIAAIKGRTIHAYHTEGAGGGHAPDIIRICGQPNVIPSSTNPTR 304

Query: 303 PYTLNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRRETIAAEDVLHDLGAFSLTSSDSQ 362
PYT+NT+ EHLDMLMVCHHL P I ED+AFAESRIR+ETIAAED+LHD+GAFS+ SSDSQ
Sbjct: 305 PYTVNTLAEHLDMLMVCHHLSPTIPEDIAFAESRIRKETIAAEDILHDIGAFSIISSDSQ 364

Query: 363 AMGRVGEVILRTWQVAHRMKVQRGALAEETGDNDNFRVKRYIAKYTINPALTHGIAHEVG 422
AMGRVGEV +RTWQ A +MK QRG L EETGDNDNFRVKRYIAKYTINPA+ HG++HE+G
Sbjct: 365 AMGRVGEVAIRTWQTADKMKRQRGRLKEETGDNDNFRVKRYIAKYTINPAIAHGLSHEIG 424

Query: 423 SIEVGKLADLVVWSPAFFGVKPATVIKGGMIAIAPMGDINASIPTPQPVHYRPMFGALGS 482
S+EVGK ADLV+W+PAFFGVKP V+ GG IA APMGD NASIPTPQPVHYRPMFGA G
Sbjct: 425 SLEVGKRADLVLWNPAFFGVKPDMVLLGGTIAAAPMGDPNASIPTPQPVHYRPMFGAYGR 484

Query: 483 ARHHCRLTFLSQAAAANGVAERLNLRSAIAVVKGCR-TVQKADMVHNSLQPNITVDAQTY 541
+R + +TF+SQA+ G+A RL + + V+ R + KA M+HNSL P+I VD +TY
Sbjct: 485 SRTNSSVTFVSQASLDAGLAGRLGVAKELVAVQNTRGGIGKASMIHNSLTPHIEVDPETY 544

Query: 542 EVRVDGELITSEPADVLPMAQRYFLF 567
EVR DGEL+T EPA VLPMAQRYFLF
Sbjct: 545 EVRADGELLTCEPATVLPMAQRYFLF 570


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45910LIPPROTEIN48270.016 Mycoplasma P48 major surface lipoprotein signature.
		>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature.

Length = 428

Score = 27.3 bits (60), Expect = 0.016
Identities = 24/66 (36%), Positives = 31/66 (46%), Gaps = 11/66 (16%)

Query: 12 ITTIGVYDWEQTIEQK----LVFDI-EIAWDNRKAAASDDVSDCLSYADISERVIAHVEG 66
I IG+ D++ E K L F+I E A+ A A LS D S+RV+A G
Sbjct: 148 IKIIGI-DFDIETEYKWFYSLQFNIKESAFTTGYAIA-----SWLSEQDESKRVVASFGG 201

Query: 67 GKFALV 72
G F V
Sbjct: 202 GAFPGV 207


10KPHS_45400KPHS_44720Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_45400-1153.225777hypothetical protein
KPHS_453900153.424884hypothetical protein
KPHS_45380-115-0.125923LysR family transcriptional regulator
KPHS_45370-119-2.433398cystathionine-gamma-lyase
KPHS_45360029-5.720576cystathionine-beta-synthase
KPHS_45350-141-8.241199putative CopG family helix-turn-helix protein
KPHS_45340-137-6.823332hypothetical protein
KPHS_45330-135-6.824113hypothetical protein
KPHS_45320-231-3.805018hypothetical protein
KPHS_45310-132-5.003364chromosome partitioning ATPase
KPHS_45300042-8.668916replicative DNA helicase
KPHS_45290044-10.308855hypothetical protein
KPHS_45280151-12.559294hypothetical protein
KPHS_45270255-13.486629hypothetical protein
KPHS_45260257-14.669337hypothetical protein
KPHS_45250259-14.748788hypothetical protein
KPHS_45240148-10.333308hypothetical protein
KPHS_45230143-8.143001hypothetical protein
KPHS_45220240-6.221448hypothetical protein
KPHS_45210332-2.635664hypothetical protein
KPHS_45200330-2.387242hypothetical protein
KPHS_45190232-1.807800hypothetical protein
KPHS_45180126-0.221616type IV B pilus protein
KPHS_45170026-0.143809hypothetical protein
KPHS_45160024-0.411258hypothetical protein
KPHS_45150024-2.027773hypothetical protein
KPHS_45140027-3.448249hypothetical protein
KPHS_45130-125-3.546182TraG-family protein
KPHS_45120032-6.284972hypothetical protein
KPHS_45110130-5.661621hypothetical protein
KPHS_45100232-5.612235hypothetical protein
KPHS_45090322-2.541243hypothetical protein
KPHS_450803210.985645hypothetical protein
KPHS_450603191.726479hypothetical protein
KPHS_450701191.804759hypothetical protein
KPHS_450500172.079996hypothetical protein
KPHS_45040-1171.726311hypothetical protein
KPHS_45030-1171.017727hypothetical protein
KPHS_45020-218-0.148880hypothetical protein
KPHS_45010-129-6.500023hypothetical protein
KPHS_45000138-10.804835hypothetical protein
KPHS_44990571-21.514083hypothetical protein
KPHS_44980776-22.799864hypothetical protein
KPHS_44970876-23.833269hypothetical protein
KPHS_44960671-21.807471hypothetical protein
KPHS_44950258-17.591764hypothetical protein
KPHS_44940139-11.069638hypothetical protein
KPHS_44930139-10.541460hypothetical protein
KPHS_44920025-6.073415hypothetical protein
KPHS_449101150.328685hypothetical protein
KPHS_44900015-0.174550hypothetical protein
KPHS_44890019-1.839453hypothetical protein
KPHS_44880123-4.762436hypothetical protein
KPHS_44870124-5.502368hypothetical protein
KPHS_44860234-7.425468hypothetical protein
KPHS_44850136-7.687421transcriptional regulator Nlp
KPHS_44840149-14.389677Xre family transcriptional regulator
KPHS_44830245-12.642274cold shock protein
KPHS_44820150-15.017606transposase InsC for insertion sequence IS903
KPHS_44810255-16.873791transcriptional regulator Nlp
KPHS_44800254-16.940968hypothetical protein
KPHS_44790253-16.542176hypothetical protein
KPHS_44780144-11.420553DNA-binding prophage protein
KPHS_44770-140-11.030169hypothetical protein
KPHS_44760136-6.698788hypothetical protein
KPHS_44750137-7.220768hypothetical protein
KPHS_44740132-6.164843hypothetical protein
KPHS_44730019-3.434912hypothetical protein
KPHS_44720-114-3.096214antirestriction protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45390DHBDHDRGNASE424e-07 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 42.3 bits (99), Expect = 4e-07
Identities = 26/132 (19%), Positives = 48/132 (36%), Gaps = 7/132 (5%)

Query: 30 GRTRGDYQVDITSQESVEA----LFAQTGEVDAIVSTTGNLHFGPLSTMTDSQFNLGLQD 85
R + D+ +++ + + G +D +V+ G L G + +++D ++
Sbjct: 56 ARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSV 115

Query: 86 KLLGQIRL--ALVGQHFLRDGGSITLVSGIVAQEPIAQGVNATTVNAGLEGFVRAAACEL 143
G ++ R GSI V A P + A F + EL
Sbjct: 116 NSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLEL 175

Query: 144 -PRGIRINLISP 154
IR N++SP
Sbjct: 176 AEYNIRCNIVSP 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45340SACTRNSFRASE300.003 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 29.5 bits (66), Expect = 0.003
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 6/65 (9%)

Query: 65 LAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDE--ARDFYLRVGFEPSP 122
+AV K KGVG AL+ A I+ A+ G+++ A FY + F
Sbjct: 95 IAVAKDYRKKGVGTALLHKA----IEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150

Query: 123 MDPMM 127
+D M+
Sbjct: 151 VDTML 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45290ARGREPRESSOR375e-05 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 37.2 bits (86), Expect = 5e-05
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 3/34 (8%)

Query: 154 IYEEQFERKVTQQELADLLTGEGYRVTQSNISRM 187
I + E TQ EL D+L +GY VTQ+ +SR
Sbjct: 14 ITANEIE---TQDELVDILKKDGYNVTQATVSRD 44


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45170RTXTOXIND330.001 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.9 bits (75), Expect = 0.001
Identities = 14/123 (11%), Positives = 43/123 (34%), Gaps = 5/123 (4%)

Query: 25 IVLGASSAFLWTQQLRLMQSSAALEQQWQTLAQQQAQFEQRIAGVETRQQ---QTEPPKP 81
+ L F + +++ ++ +++Q+ T Q+ Q E + +
Sbjct: 168 LKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYE 227

Query: 82 LVSAVTQEQLT--QALAENRQEVNQQHAQLQQQMTSVTQRVEVLEQRDGALSGQWLELKQ 139
+S V + +L +L + + + + + V + + + + L K+
Sbjct: 228 NLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKE 287

Query: 140 QMA 142
+
Sbjct: 288 EYQ 290



Score = 32.5 bits (74), Expect = 0.002
Identities = 10/90 (11%), Positives = 27/90 (30%), Gaps = 15/90 (16%)

Query: 37 QQLRLMQSSAALEQQWQTLAQQQAQFEQRIAGVETRQQQTEPPKPLVSAVTQEQLTQALA 96
+ +++ + L ++Q EQ + +E+
Sbjct: 250 AKHAVLEQENKYVEAVNELRVYKSQLEQ------IESEILS---------AKEEYQLVTQ 294

Query: 97 ENRQEVNQQHAQLQQQMTSVTQRVEVLEQR 126
+ E+ + Q + +T + E+R
Sbjct: 295 LFKNEILDKLRQTTDNIGLLTLELAKNEER 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_45020PF01540320.007 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

Length = 475

Score = 31.6 bits (71), Expect = 0.007
Identities = 26/113 (23%), Positives = 55/113 (48%), Gaps = 9/113 (7%)

Query: 39 QTGDSVVADLTQEELKALGVEGDTPQDTLRTLVGRMRTIQNKQVELDRDNKALAEENARL 98
+T + +A+ T+ K G GD P + L + +++Q ++D+ NK +A+EN ++
Sbjct: 66 ETLNKEIAEATKS-FKEAGSYGDYPA-IISKLSAAVENAKSEQQKVDQANKKIADENLKI 123

Query: 99 AQGESGL---DNRI---SAAVAKAQKEAEQKAEGVKEE-QRQLSKALDELKER 144
+G L +I + +A + E K + E ++QL ++ L ++
Sbjct: 124 KEGAKELLKLSEKIQSFADTIALTITKLEGKKFQIDETFKKQLISTIELLNKK 176


11KPHS_44320KPHS_44210Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_443202142.612106major facilitator superfamily transporter MFS_1
KPHS_443101143.128062putative cupin domain protein
KPHS_443002132.579737putative hydrolase
KPHS_442901131.975603short chain dehydrogenase
KPHS_44280-1161.969382hypothetical protein
KPHS_44270-1151.067927putative aldehyde dehydrogenase
KPHS_442601220.024228thiamine pyrophosphate enzyme
KPHS_44250232-1.371786outer membrane protein OprE3
KPHS_44240334-1.029443hypothetical protein
KPHS_44230435-1.458381transketolase
KPHS_44220227-3.015913D-erythrose 4-phosphate dehydrogenase
KPHS_44210231-2.534045phosphoglycerate kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44320TCRTETB424e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 41.8 bits (98), Expect = 4e-06
Identities = 26/132 (19%), Positives = 57/132 (43%), Gaps = 3/132 (2%)

Query: 75 AFLLSYGFSSVLLSGLGDRIAPLRLLTGMMVVWCILMVIMGFTHNYALMVTLRILLGIAE 134
AF+L++ + + L D++ RLL +++ C VI H++ ++ + + A
Sbjct: 57 AFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAG 116

Query: 135 GPLFPLAFAVVRHTF-PQRLQARATMLWLLGTPVGAALGFPLSIWLLNTFGWQSTFFVM- 192
FP VV + P+ + +A L +G +G + + + W +
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM 176

Query: 193 -AMLTIPVLIFV 203
++T+P L+ +
Sbjct: 177 ITIITVPFLMKL 188


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44300PF06057290.016 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 29.0 bits (65), Expect = 0.016
Identities = 21/88 (23%), Positives = 33/88 (37%), Gaps = 8/88 (9%)

Query: 28 KQMALPGYRVLAWDMPGYGESPMLAATPAD-AGDYADALARMLDRAGVEQTILVGHSLGA 86
+ G+ V+ W Y P D D + + G ++ IL+G+S GA
Sbjct: 72 GILQQQGWPVVGWSSLKYYWK---QKDPKDVTQDTLAIIDKYQAEFGTQKVILIGYSFGA 128

Query: 87 LVAAAFAAKYP----QRVLYLVLADVAQ 110
V + P + VL VL +Q
Sbjct: 129 EVIPFVLNEMPARYRKNVLGAVLLSPSQ 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44290DHBDHDRGNASE1081e-30 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 108 bits (272), Expect = 1e-30
Identities = 77/262 (29%), Positives = 118/262 (45%), Gaps = 9/262 (3%)

Query: 1 MNAQ-IEGRVAVVTGGSSGIGFETLRLLLGEGAKVAFCGRNPDRLASAHAALQNE--YPE 57
MNA+ IEG++A +TG + GIG R L +GA +A NP++L ++L+ E + E
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 58 GEVFSWRCDVLNEAEVEAFAAAVAARFGGVDMLINNAGQGYVAHFADTPREAWLHEAELK 117
++ DV + A ++ A + G +D+L+N AG E W +
Sbjct: 61 ----AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVN 116

Query: 118 LFGVINPVKAFQSLLEASDIASITCVNSLLALQPEEHMIATSAARAALLNMTLTLSKELV 177
GV N ++ + SI V S A P M A ++++AA + T L EL
Sbjct: 117 STGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 178 DKGIRVNSILLGMVESGQWQRRFESRSDKSQSWQQWTADIARKRGIPMARLGKPQEPAQA 237
+ IR N + G E+ + + Q + K GIP+ +L KP + A A
Sbjct: 177 EYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETF--KTGIPLKKLAKPSDIADA 234

Query: 238 LLFLASPLASFTTGAALDVSGG 259
+LFL S A T L V GG
Sbjct: 235 VLFLVSGQAGHITMHNLCVDGG 256


12KPHS_43720KPHS_43570Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_437200133.706892Ars family arsenical pump
KPHS_43710-1154.221810arsenate reductase
KPHS_43700-1144.073225di-/tripeptide transporter
KPHS_43690-1154.513683hypothetical protein
KPHS_43680-1154.285143putative outer membrane efflux protein MdtP
KPHS_43670-1133.271565multidrug efflux system protein MdtO
KPHS_43660-2151.455168multidrug resistance protein MdtN
KPHS_436500140.817850hypothetical protein
KPHS_436400141.356246hypothetical protein
KPHS_436302162.456170acyltransferase domain-containing protein
KPHS_436202142.472836putative fimbrial protein
KPHS_436103152.339458fimbrial protein FimH
KPHS_436003142.071860fimbrial morphology protein
KPHS_435903141.117100type 1 fimbrial minor component
KPHS_435802150.173122outer membrane protein for export and assembly
KPHS_43570-123-3.544138periplasmic chaperone
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43700TCRTETB310.010 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.0 bits (70), Expect = 0.010
Identities = 33/188 (17%), Positives = 73/188 (38%), Gaps = 12/188 (6%)

Query: 33 SFYGIRPLLILFMAATVYDGGMGLARENASAIVGIFAGSMYLAALPGGWLADNWLGQQRA 92
SF+ + ++L ++ + + + F + + G L+D LG +R
Sbjct: 23 SFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ-LGIKRL 81

Query: 93 VWYGSILIALGHLSIALSAWLGNDLFFIGLMFIVL---GSGLFKTCISVMVGTLYKKGDA 149
+ +G I+ G ++ ++G+ F + +M + G+ F + V+V K
Sbjct: 82 LLFGIIINCFG----SVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK--E 135

Query: 150 RRDGGFSLFYMGINIGSFIAPLISGWLIKSHGWHWGFGIGGIGMLVALIIFRVFAVPSMK 209
R F L + +G + P I G + +H HW + + + + + F + +
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGG--MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEV 193

Query: 210 RYDAEVGL 217
R +
Sbjct: 194 RIKGHFDI 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43670GPOSANCHOR300.033 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 30.0 bits (67), Expect = 0.033
Identities = 33/148 (22%), Positives = 50/148 (33%), Gaps = 30/148 (20%)

Query: 489 DSEAPQLKTRLAALYRRLADCLAAPKEAVPLAPLLVAFTDSEALIHRVRAEPLGTYAHPW 548
+ + ++LAAL + + + K L A ++EA + E L
Sbjct: 399 EKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEA---KALKEKL------- 448

Query: 549 PQAKDWPMRATLAQAEEIARLSEGYRLNAAPGDPTLAR-------CAEQLRRYAERIEQE 601
AK QAEE+A+L G ++ D A Q + +
Sbjct: 449 --AK---------QAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAP 497

Query: 602 ATAPGEQL--TAELTNPFGPALAAALAA 627
QL T E NPF A A + A
Sbjct: 498 MKETKRQLPSTGETANPFFTAAALTVMA 525


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43660RTXTOXIND672e-14 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 66.8 bits (163), Expect = 2e-14
Identities = 50/362 (13%), Positives = 106/362 (29%), Gaps = 81/362 (22%)

Query: 11 KKWPLLALVLAAILALILVIWQL-----QTSPETNDAYVYADTIDVVPEVSGRIVEMPIR 65
+ P L +I I + + + ++ P + + E+ ++
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 66 DNQRVRKGDLLFRIDPRP---------------------YQAMLDDA------------- 91
+ + VRKGD+L ++ YQ +
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 92 ------------------KARLTTLDAQIMLTQRTIKAQEYNAQSVAAAVERARALVKQT 133
K + +T Q + + + +V A + R L +
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 134 TSTRIRLEPLVPQGFASQEDLDQARTAEKAARAELEATLLQAKQASAAVTGVDAMVAQRA 193
S L+ + ++ + + A EL Q +Q + +
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 194 GVL-------------------AQIALAELHLEFTEVRAPFNGVVVALKT-TVGQYASAL 233
+ ++A E + + +RAP + V LK T G +
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 234 KPVFTLL-DDDRWYVIANFRETDLNNVRPGVAARITVMT-NHNRT--FNGVVDSVGSGVL 289
+ + ++ +DD V A + D+ + G A I V + R G V ++ +
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAI 413

Query: 290 PE 291
+
Sbjct: 414 ED 415


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43630SACTRNSFRASE280.002 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.4 bits (63), Expect = 0.002
Identities = 11/55 (20%), Positives = 23/55 (41%)

Query: 11 YVNDAQGNQVAEIVFVPTGEHLSIIEHTDVDPSLKGQGVGKQLVAKVVEKMRQEQ 65
++ + N + I ++IE V + +GVG L+ K +E ++
Sbjct: 68 FLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENH 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43580PF005779760.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 976 bits (2525), Expect = 0.0
Identities = 665/866 (76%), Positives = 758/866 (87%), Gaps = 6/866 (0%)

Query: 11 LGCRTARRLVSPALALWLC------SQPFAARADLYFNPRFLADDPAAVADLSGFEKGQE 64
C R+ + L +Q + A+LYFNPRFLADDP AVADLS FE GQE
Sbjct: 13 TQCLHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQE 72

Query: 65 VPPGTYRVDIYLNNGFMTTRDVTFQADAQGHGLSPCLTRGQLASMGVDTGRVPGMATLDS 124
+PPGTYRVDIYLNNG+M TRDVTF G+ PCLTR QLASMG++T V GM L
Sbjct: 73 LPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLAD 132

Query: 125 TACVPLTTLISEATTRFDVGQQRLYLTVPQAFMGNHARGYIPPELWDNGITAGLINYNFT 184
ACVPLT++I +AT + DVGQQRL LT+PQAFM N ARGYIPPELWD GI AGL+NYNF+
Sbjct: 133 DACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFS 192

Query: 185 GNNAHNTTGGSSRYAYLNLQSGLNIGAWRLRDNSTWSYSSGGSTSSNENRWQHVNSWLER 244
GN+ N GG+S YAYLNLQSGLNIGAWRLRDN+TWSY+S S+S ++N+WQH+N+WLER
Sbjct: 193 GNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLER 252

Query: 245 DITPLRSRLTLGDSYTNGDVFDGINFRGAQLASDDNMLPDSQKGFAPVIHGIARGTAQVS 304
DI PLRSRLTLGD YT GD+FDGINFRGAQLASDDNMLPDSQ+GFAPVIHGIARGTAQV+
Sbjct: 253 DIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVT 312

Query: 305 IRQNGYEIYQSTVPPGPFTIDDLYAAGNGGDLQVTIKEADGSRQVFSVPWSTVPVLQREG 364
I+QNGY+IY STVPPGPFTI+D+YAAGN GDLQVTIKEADGS Q+F+VP+S+VP+LQREG
Sbjct: 313 IKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREG 372

Query: 365 HTRFALTAGEYRSGNSQQETPDFFQGTVMHGLPAGWTLYGGTQLADRYRAFNLGVGKNMG 424
HTR+++TAGEYRSGN+QQE P FFQ T++HGLPAGWT+YGGTQLADRYRAFN G+GKNMG
Sbjct: 373 HTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMG 432

Query: 425 YFGALSLDITQANATLADDSEHQGQSVRFLYNKSLDETGTNLQLVGYRYSTRGYYNFADT 484
GALS+D+TQAN+TL DDS+H GQSVRFLYNKSL+E+GTN+QLVGYRYST GY+NFADT
Sbjct: 433 ALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADT 492

Query: 485 TYRRMSGYSVETQDGVIQVKPKFTDYYNLAYSKRGKVQLSVTQQLGRTATLYLSGSHQTY 544
TY RM+GY++ETQDGVIQVKPKFTDYYNLAY+KRGK+QL+VTQQLGRT+TLYLSGSHQTY
Sbjct: 493 TYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTY 552

Query: 545 WGTDDADEQLQAGLNAAVDDINWSLSYSLTKNAWQQGRDQMLAININIPFSHWLRSDSRS 604
WGT + DEQ QAGLN A +DINW+LSYSLTKNAWQ+GRDQMLA+N+NIPFSHWLRSDS+S
Sbjct: 553 WGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKS 612

Query: 605 VWRHASASYSLSHDLNGRMTNLAGLYGTLLEDNNLSYSMQTGYAGGGNGDNGSTGYTALN 664
WRHASASYS+SHDLNGRMTNLAG+YGTLLEDNNLSYS+QTGYAGGG+G++GSTGY LN
Sbjct: 613 QWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLN 672

Query: 665 YRGGYGNANVGYSRSDGFKQLYYGVSGGVLAHANGITLSQPLNDTVVLVKAPGAGGVKVE 724
YRGGYGNAN+GYS SD KQLYYGVSGGVLAHANG+TL QPLNDTVVLVKAPGA KVE
Sbjct: 673 YRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVE 732

Query: 725 NQTGVRTDWRGYAVLPYATEYRENRIALDTNTLADNVDLDDAVVSVVPTHGAIVRANFNA 784
NQTGVRTDWRGYAVLPYATEYRENR+ALDTNTLADNVDLD+AV +VVPT GAIVRA F A
Sbjct: 733 NQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKA 792

Query: 785 QVGMKILMTLTHRGKPVPFGALATGDSNQSGSIVADNGQVYLSGMPLAGKVRVKWGDGPD 844
+VG+K+LMTLTH KP+PFGA+ T +S+QS IVADNGQVYLSGMPLAGKV+VKWG+ +
Sbjct: 793 RVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEEN 852

Query: 845 AQCVADYRLPPESQQQALSQLSVACR 870
A CVA+Y+LPPESQQQ L+QLS CR
Sbjct: 853 AHCVANYQLPPESQQQLLTQLSAECR 878


13KPHS_43480KPHS_43420Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_43480413-1.482773hypothetical protein
KPHS_43470414-2.499669MrkA fimbrial protein
KPHS_43460215-3.148274MrkB fimbrial protein
KPHS_43450015-4.168035putative fimbrial usher protein
KPHS_43440121-4.882642putative fimbrial-like protein
KPHS_43430-220-5.044784putative fimbrial-like protein
KPHS_43420-219-4.814688hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43450PF005776640.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 664 bits (1715), Expect = 0.0
Identities = 263/846 (31%), Positives = 403/846 (47%), Gaps = 47/846 (5%)

Query: 10 RLSTAIAIALCCFPPFSSGQENPGTVYQFNDGFIVG-SREKVDLSRFSTS-AITEGTYSL 67
+ L F++ FN F+ + DLSRF + GTY +
Sbjct: 21 HRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRV 80

Query: 68 DVYTNDEWKGRYDLR-IARDKDGRLGVCYTKAMLAQYGIAAEKLNPQLSEQEGYCGSLKS 126
D+Y N+ + D+ D + + C T+A LA G+ ++ + C L S
Sbjct: 81 DIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTS 140

Query: 127 WRNEENVKDNLVQSSLRLNISVPQIYEDQRLKNYVSPEFWDKGITALNLGWMANAWNSHT 186
+ L RLN+++PQ + R + Y+ PE WD GI A L + + +
Sbjct: 141 MI--HDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSG--NSV 196

Query: 187 SSVGGSDNSSAYLGVNAGLSWDGWLLKHIGNLNWQQQQG----KAHWNSNQTYLQRPIPQ 242
+ G ++ AYL + +GL+ W L+ ++ K W T+L+R I
Sbjct: 197 QNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIP 256

Query: 243 LNSIVSGGQIFTNGEFFDTIGLRGVNLSTDDNMFPDGMRSYAPEIRGVAQSNALVTVRQG 302
L S ++ G +T G+ FD I RG L++DDNM PD R +AP I G+A+ A VT++Q
Sbjct: 257 LRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQN 316

Query: 303 SNIIYQTTVPPGPFTLQDVYPSGYGSDLEVSVKEADGSVEVFSVPYASVAQLLRPGMTRY 362
IY +TVPPGPFT+ D+Y +G DL+V++KEADGS ++F+VPY+SV L R G TRY
Sbjct: 317 GYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRY 376

Query: 363 ALSAGKV-DDSALRNKPMLYQATWQHGINNLLTGYTGVTGFDDYQAFLVGTGMNTG-IGA 420
+++AG+ +A + KP +Q+T HG+ T Y G D Y+AF G G N G +GA
Sbjct: 377 SITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGA 436

Query: 421 LSFDVTHSRLKS-DAHDDSGQSYRATFNRMFTDTQTSIVLAAYRYSTKGYYNLNDALYA- 478
LS D+T + D GQS R +N+ ++ T+I L YRYST GY+N D Y+
Sbjct: 437 LSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSR 496

Query: 479 -------------VDQEKNSRSNYTLWRQKNGMTFTVNQNLPDGWGGFYLSGRISDYWNR 525
+ K + + ++ + TV Q L YLSG YW
Sbjct: 497 MNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGR-TSTLYLSGSHQTYWGT 555

Query: 526 SGTEKQYQVSYNNSFGRLSWSASAQRVYTPDSSGHRRDDRISLNFSYPL--WFGDN---- 579
S ++Q+Q N +F ++W+ S T ++ RD ++LN + P W +
Sbjct: 556 SNVDEQFQAGLNTAFEDINWTLSYSL--TKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQ 613

Query: 580 -RTANLTSNTSFNNSRFASSQIGINGSLDSENNLNYGVSTTTATGGQHD----VALNGSY 634
R A+ + + S + + ++ G+ G+L +NNL+Y V T A GG + +Y
Sbjct: 614 WRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNY 673

Query: 635 RTPWTTLNGSYSQGEGYRQSGIGASGTMIAHSGGVVLSPESGSTMALIEAKDAAGAMLPG 694
R + N YS + +Q G SG ++AH+ GV L T+ L++A A A +
Sbjct: 674 RGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVEN 733

Query: 695 SPGTRVDSNGYAILPYLRPYRINAVEIDPKGSHDDVAFDRTVAQVVPWEGSVVKVAFGTK 754
G R D GYA+LPY YR N V +D D+V D VA VVP G++V+ F +
Sbjct: 734 QTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKAR 793

Query: 755 VQNNLTLQARQANHEPLPFAASIFSPDGKEIGVVGQGSMMFISDANAK-RAIVKW---SG 810
V L + N +PLPF A + S + G+V +++S + VKW
Sbjct: 794 VGIKLLMTLTHNN-KPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEEN 852

Query: 811 GQCSVD 816
C +
Sbjct: 853 AHCVAN 858


14KPHS_43010KPHS_42760Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_430101173.820813fused phosphoenolpyruvate-protein
KPHS_430001183.706683prolipoprotein diacylglyceryl transferase
KPHS_429903174.159820thymidylate synthase
KPHS_429802153.537000prepilin peptidase-dependent protein A
KPHS_429702154.127929prepilin peptidase dependent protein B
KPHS_429602164.650531hypothetical protein
KPHS_429502154.356156hypothetical protein
KPHS_429402153.872477exonuclease V (RecBCD complex) subunit gamma
KPHS_429301153.661737protease III
KPHS_429201164.695844exonuclease V subunit beta
KPHS_429102174.527825exonuclease V subunit alpha
KPHS_429000162.854840hypothetical protein
KPHS_428900163.051149N-acetylglutamate synthase
KPHS_42880-1163.778556N-acetylmuramoyl-L-alanine amidase
KPHS_428702194.664910threonine-phosphate decarboxylase
KPHS_428601164.691834propanediol utilization
KPHS_428500144.632515propionate kinase
KPHS_42840-1144.284911propanediol utilization
KPHS_428300154.675906propanediol utilization polyhedral bodies
KPHS_428200154.877236propanediol utilization polyhedral bodies
KPHS_428100155.090207propanediol utilization polyhedral bodies
KPHS_428002164.517210propanol dehydrogenase
KPHS_427900174.366333propanediol utilization: CoA-dependent
KPHS_427802184.881607PduO protein
KPHS_427700193.912258propanediol utilization polyhedral bodies
KPHS_42760-1163.073152propanediol utilization
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43010PHPHTRNFRASE5930.0 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 593 bits (1530), Expect = 0.0
Identities = 185/571 (32%), Positives = 314/571 (54%), Gaps = 7/571 (1%)

Query: 168 QTRIRALPASSGVAIAEGWMDVSLPLMEQVYEASTLDTASERERLTGALEEAANEFRRYS 227
+I + ASSGVAIA+ ++ + + + + S D ++E E+LT ALE++ E R
Sbjct: 2 HHKITGIAASSGVAIAKAFIHLEPNV--DIEKTSITDVSTEIEKLTAALEKSKEELRAIK 59

Query: 228 KRYAAGAQKETAAIFDLYSHLLSDARLRRELFAEVDKGAV-AEWAVKKIIEKFAEQFAAL 286
+ A + A IF + +L D L + +++ + AE+A+K++ + F F ++
Sbjct: 60 DQTEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESM 119

Query: 287 SDGYLKERAGDLRTLGQRLLFHLDDS-IQGPNTWPARIILVADELSATTLAEVPQDRLAG 345
+ Y+KERA D+R + +R+L HL T +++A++L+ + A++ + + G
Sbjct: 120 DNEYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKG 179

Query: 346 VVVRDGAANSHAAIMVRALGIPTVMGA-DIQPSLLHGHTLIVDGYRGELLVDPEPVLLQE 404
G SH+AIM R+L IP V+G ++ + HG +IVDG G ++V+P ++
Sbjct: 180 FATDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKA 239

Query: 405 YQRLISEENELSRLAEDDLQRASELKSGERVKVMLNAGLSPEHEEKLGSFVDGIGLYRTE 464
Y+ + + + + S K G V++ N G + + L + +GIGLYRTE
Sbjct: 240 YEEKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTE 299

Query: 465 IPFMLQSGFPSEEEQVAQYQGMLQMFNSKPVTLRTLDIGADKQLPYMPISEE-NPCLGWR 523
+M + P+EEEQ Y+ ++Q + KPV +RTLDIG DK+L Y+ + +E NP LG+R
Sbjct: 300 FLYMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFR 359

Query: 524 GIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVTSLEEVDEARRLIDRASREVEEMI 583
IR+ L++ +IF Q+RA+LRA + GNL ++ PM+ +LEE+ +A+ ++ ++
Sbjct: 360 AIRLCLEKQDIFRTQLRALLRA-STYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEG 418

Query: 584 GYAIPRPRLGVMLEVPSMVFMLPQLASRIDFISVGTNDLTQYLLAVDRNNTRVASMYDSL 643
+G+M+E+PS A +DF S+GTNDL QY +A DR N RV+ +Y
Sbjct: 419 VDVSDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPY 478

Query: 644 HPAVLRALAMIAHDAERFGIDLRLCGEMAGDPMCVTILIGLGYRHLSMNGRSVARVKYLL 703
HPA+LR + M+ A G + +CGEMAGD + + +L+GLG SM+ S+ + L
Sbjct: 479 HPAILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQL 538

Query: 704 RRIDIEEAQELSRRSLDAQMTAEVRHQVAAF 734
++ EE + ++++L EV V
Sbjct: 539 LKLSKEELKPFAQKALMLDTAEEVEQLVKKT 569


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_42980BCTERIALGSPH290.008 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 28.8 bits (64), Expect = 0.008
Identities = 19/96 (19%), Positives = 36/96 (37%), Gaps = 1/96 (1%)

Query: 31 REHGYTLMETLVTLTLMMILSVGGLYGWQRWQQQQRLWQTAVQVRDFLLFLRDDANAYNR 90
R+ G+TL+E ++ L LM + + L + + A + L F++ +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLA-RFEAQLRFVQQRGLQTGQ 60

Query: 91 DRVLRVGQDEVGWCLSAEGEGPDCASGTSFTLRPRW 126
+ V D + + +G D A RW
Sbjct: 61 FFGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRW 96


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_42950BCTERIALGSPH260.035 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 25.7 bits (56), Expect = 0.035
Identities = 10/23 (43%), Positives = 15/23 (65%)

Query: 7 RQRGFSLPETVLAMALMVLTVTA 29
RQRGF+L E +L + LM ++
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGM 24


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_42850ACETATEKNASE5590.0 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 559 bits (1441), Expect = 0.0
Identities = 198/395 (50%), Positives = 270/395 (68%), Gaps = 5/395 (1%)

Query: 4 KIMAINAGSSSLKFQLLNMPQGALLCQGLIERIGLPEARFTLKTSAQKWQETLPIADHHE 63
KI+ IN GSSSLK+QL+ G +L +GL ERIG+ ++ T + +K + + DH +
Sbjct: 2 KILVINCGSSSLKYQLIESKDGNVLAKGLAERIGINDSLLTHNANGEKIKIKKDMKDHKD 61

Query: 64 AVTLLLEALTGR--GILSSLQEIDGVGHRVAHGGERFKDAALVCDDTLREIERLAELAPL 121
A+ L+L+AL G++ + EID VGHRV HGGE F + L+ DD L+ I ELAPL
Sbjct: 62 AIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGEYFTSSVLITDDVLKAITDCIELAPL 121

Query: 122 HNPVNALGIRLFRQLLPAVPAVAVFDTAFHQTLAPEAWLYPLPWRYYAELGIRRYGFHGT 181
HNP N GI+ Q++P VP VAVFDTAFHQT+ A+LYP+P+ YY + IR+YGFHGT
Sbjct: 122 HNPANIEGIKACTQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRKYGFHGT 181

Query: 182 SHHYVSSALAEKLGVPLSALRVVSCHLGNGCSVCAIKGGQSVNTSMGFTPQSGVMMGTRS 241
SH YVS AE L P+ +L++++CHLGNG S+ A+K G+S++TSMGFTP G+ MGTRS
Sbjct: 182 SHKYVSQRAAEILNKPIESLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGLAMGTRS 241

Query: 242 GDIDPSILPWLVEKEGKSAQQLSQLLNNESGLLGVSGVSSDYRDVEQAADA-GNERAALA 300
G IDPSI+ +L+EKE SA+++ +LN +SG+ G+SG+SSD+RD+E AA G++RA LA
Sbjct: 242 GSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRAQLA 301

Query: 301 LSLFAERIRATIGSYIMQMGGLDALIFTGGIGENSARARAAICRNLHFLGLALDDEKNQR 360
L++FA R++ TIGSY MGG+D ++FT GIGEN R I L FLG LD EKN+
Sbjct: 302 LNVFAYRVKKTIGSYAAAMGGVDVIVFTAGIGENGPEIREFILDGLEFLGFKLDKEKNKV 361

Query: 361 SA--TFIQADNALVKVAVINTNEELMIARDVMRLA 393
I ++ V V V+ TNEE MIA+D ++
Sbjct: 362 RGEEAIISTADSKVNVMVVPTNEEYMIAKDTEKIV 396


15KPHS_42650KPHS_42460Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_426500133.567928transposase InsC for insertion sequence IS903
KPHS_426403185.424281cobyrinic Acid a,c-diamide synthase
KPHS_426302195.643372cobalamin biosynthesis protein CobD
KPHS_426200215.984118precorrin-8X methylmutase
KPHS_426100195.385686cobalt-precorrin-6A synthase
KPHS_426000206.085705hypothetical protein
KPHS_42590-1195.250381cobalt-precorrin-6Y C(15)-methyltransferase
KPHS_42580-1185.558222hypothetical protein
KPHS_425701185.373453cobalamin biosynthesis protein CbiG
KPHS_425602194.343524hypothetical protein
KPHS_425503194.725199hypothetical protein
KPHS_425403224.062261hypothetical protein
KPHS_425303234.649695hypothetical protein
KPHS_425203224.179779hypothetical protein
KPHS_425103224.897652cobalt transport protein CbiN
KPHS_425002194.419012hypothetical protein
KPHS_424901184.320303cobalt transporter ATP-binding subunit
KPHS_424800164.437267cobyric acid synthase
KPHS_42470-1154.893935adenosylcobinamide
KPHS_42460-1144.235740nicotinate-nucleotide-dimethylbenzimidazole
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_42560PF05272300.007 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.007
Identities = 9/58 (15%), Positives = 17/58 (29%), Gaps = 1/58 (1%)

Query: 152 ADFVICFYNPRSRGREGHLARAFTLLAASKSADTPVGVVKSAGRKKQEKWLTTLGEMD 209
D+ + G+ + L S + +G K + + L EM
Sbjct: 595 FDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFD-IGTGKDSYEQIAGIVAYELSEMT 651


16KPHS_42330KPHS_42230Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_42330-1153.343861fucose permease
KPHS_423200164.822654hypothetical protein
KPHS_423100155.441453L-fuculose phosphate aldolase
KPHS_423000147.023832L-1,2-propanediol oxidoreductase
KPHS_422901166.767833exonuclease IX
KPHS_422802156.858165sigma-54 dependent transcriptional regulator
KPHS_422701156.365943hypothetical protein
KPHS_422600145.540690alkanesulfonate monooxygenase
KPHS_42250-1134.518531FAD-dependent oxidoreductase
KPHS_42240-1133.449761NtaA/SnaA/SoxA family monooxygenase
KPHS_42230-3113.380052putative D- and L-methionine transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_42280HTHFIS379e-127 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 379 bits (974), Expect = e-127
Identities = 141/356 (39%), Positives = 197/356 (55%), Gaps = 24/356 (6%)

Query: 4 PESPSTAPALI--DPASKAFQSLLDKLAPTEATVLIVGETGTGKEVVARYLHHHSARRQQ 61
+ L+ A + +L +L T+ T++I GE+GTGKE+VAR LH + RR
Sbjct: 130 EDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNG 189

Query: 62 PFLAVNCGALTESLAEAELFGHEKGAFTGAQQGQPGWFEAAEGGTLLLDEIGELSLPLQV 121
PF+A+N A+ L E+ELFGHEKGAFTGAQ G FE AEGGTL LDEIG++ + Q
Sbjct: 190 PFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQT 249

Query: 122 KLLRVLQEREITRVGSRKAIKVNVRVIAATHVDLAQAIRERRFREDLYYRLNIAVVPLPP 181
+LLRVLQ+ E T VG R I+ +VR++AAT+ DL Q+I + FREDLYYRLN+ + LPP
Sbjct: 250 RLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPP 309

Query: 182 LRQRRQDIPLLAHHFLSLYARRLGRPTLRLAPESLARLMDYSWPGNIRELENTLHNAVLL 241
LR R +DIP L HF+ + G R E+L + + WPGN+RELEN + L
Sbjct: 310 LRDRAEDIPDLVRHFVQQA-EKEGLDVKRFDQEALELMKAHPWPGNVRELENLVRRLTAL 368

Query: 242 SKEEEISPAQLRLATLNDAP-----------------GPASDHELDDFIRHQLALPGEPL 284
++ I+ + ++ P ++ F ALP L
Sbjct: 369 YPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGL 428

Query: 285 WQRVTSA----LIRHAMAHCDDNQSQAAELLGISRHTLRTQLANLGLIKSRRRPPA 336
+ RV + LI A+ NQ +AA+LLG++R+TLR ++ LG+ R A
Sbjct: 429 YDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSSRSA 484


17KPHS_41690KPHS_41540Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_416901123.278842DNA mismatch repair protein
KPHS_416802132.963499hypothetical protein
KPHS_416701163.384103type VI secretion system effector
KPHS_416601204.835379hemin transport system ATP-binding component
KPHS_416500184.651626vtamin B12-transporter permease
KPHS_41640-1162.545409hemin ABC superfamily ATP binding cassette
KPHS_41630-1161.657259S-adenosylmethionine-dependent
KPHS_416200161.060993outer membrane porin for ferric enterobactin and
KPHS_416101130.778451hypothetical protein
KPHS_416001141.069631acetolactate synthase III large subunit
KPHS_415901140.136492putative receptor protein
KPHS_415802152.149858putative LysR-family transcriptional regulator
KPHS_415701153.213679hypothetical protein
KPHS_415601163.814542iron transporter: fur regulated
KPHS_415501163.882686iron ABC superfamily ATP binding cassette
KPHS_415401173.376041ABC transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_41660PF05272280.049 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 27.7 bits (61), Expect = 0.049
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 4/42 (9%)

Query: 15 GKRQIIDNVSVALRGG----EMTALIGPNGAGKSTLLRLLTG 52
GK ++ +V+ + G L G G GKSTL+ L G
Sbjct: 577 GKYILMGHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLVG 618


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_41640FERRIBNDNGPP375e-05 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 36.8 bits (85), Expect = 5e-05
Identities = 44/191 (23%), Positives = 75/191 (39%), Gaps = 16/191 (8%)

Query: 52 PPAAQKLPDVGYLRQLNAEGILALRPQLVLASAQAQPSLVLHKVQASGVKVVNVPGGESL 111
PP + DVG + N E + ++P ++ SA PS + A G G + L
Sbjct: 72 PPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPL 131

Query: 112 SAIDNKVAVIAEALGKTAAGDALRQQLQQQIAAIPTQPV---AKRVLFILSHGGMNTLVA 168
+ + +A+ L +A + Q + I ++ + V A+ +L + LV
Sbjct: 132 AMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVF 191

Query: 169 GQHTAADSAIRAAGLQNAMQG---FDHYRAMSQEGVAA-SQADLVVISADGLKGMGGEAG 224
G ++ + G+ NA QG F A+S + +AA D++ D K M
Sbjct: 192 GPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDM----- 246

Query: 225 LWKLPGLAQTP 235
L TP
Sbjct: 247 ----DALMATP 253


18KPHS_41420KPHS_41200Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_41420-1193.966617formate hydrogen-lyase transcriptional
KPHS_414100184.629330hypothetical protein
KPHS_414000182.568989hydrogenase expression/formation protein
KPHS_413901202.298289hydrogenase isoenzymes formation protein
KPHS_413702164.155820hydrogenase nickel incorporation protein HypB
KPHS_413803154.162352hypothetical protein
KPHS_413602223.924348hydrogenase nickel incorporation protein
KPHS_413501233.877111formate hydrogenlyase regulatory protein HycA
KPHS_413400224.256632formate hydrogenlyase subunit B
KPHS_413301243.990125formate hydrogenlyase subunit 3
KPHS_413200222.729969hydrogenase 3 membrane subunit
KPHS_413101222.667848hydrogenase 3, large subunit (part of FHL
KPHS_41300-1181.565428formate hydrogenlyase complex iron-sulfur
KPHS_412900182.191332hypothetical protein
KPHS_41280-1181.381058processing of HycE (part of the FHL complex)
KPHS_41270-2171.799056hydrogenase 3 large subunit C-terminal protease
KPHS_41260-2171.830964hypothetical protein
KPHS_412500153.853926cryptic 6-phospho-beta-glucosidase
KPHS_412400154.775324cellobiose/arbutin/salicin-specific PTS system
KPHS_412200144.691906hypothetical protein
KPHS_41230-1143.965121ascBF operon repressor
KPHS_412101143.328673electron transport protein HydN
KPHS_412001133.782108hydrogenase maturation protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_41420HTHFIS378e-126 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 378 bits (971), Expect = e-126
Identities = 137/373 (36%), Positives = 201/373 (53%), Gaps = 41/373 (10%)

Query: 345 YREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAQSDSTVLILG 404
E+ + R + E +L + + ++GRS AM + + + + Q+D T++I G
Sbjct: 108 LTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITG 167

Query: 405 ETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRF 464
E+GTGKEL+ARA+H+ R V +N AA+P L+ES+LFGHE+GAFTGA + GRF
Sbjct: 168 ESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRF 227

Query: 465 ELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMV 524
E A+ +LFLDE+GDMP++ Q +LLRVLQ+ E+ +G I++DVR++AATN+DLKQ +
Sbjct: 228 EQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSI 287

Query: 525 IDREFRSDLYYRLNVFPIHLPPLRERPDDIPLLVKAFTFKIARRMGRNIDSIPAETLRTL 584
FR DLYYRLNV P+ LPPLR+R +DIP LV+ F + A + G ++ E L +
Sbjct: 288 NQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFV-QQAEKEGLDVKRFDQEALELM 346

Query: 585 TRMEWPGNVRELENVIERAVLLTRGNVLQ------------------------------- 613
WPGNVRELEN++ R L +V+
Sbjct: 347 KAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQ 406

Query: 614 -----LSLPERDIVEAPRTPAVLPEEGED-EYQLIVRVLKESNGVVAGPKGAAQRLGLKR 667
+ +A + + EY LI+ L + G AA LGL R
Sbjct: 407 AVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQI---KAADLLGLNR 463

Query: 668 TTLLSRMKRLGIN 680
TL +++ LG++
Sbjct: 464 NTLRKKIRELGVS 476


19KPHS_41110KPHS_41010Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_41110-3183.121659sorbitol-6-phosphate dehydrogenase
KPHS_41100-1173.979119glucitol/sorbitol-specific PTS system component
KPHS_41090-1163.524708PTS family glucitol/sorbitol porter, IIB
KPHS_41080-1163.399005PTS family enzyme IIC,
KPHS_41070-1152.887133lytic murein transglycosylase B
KPHS_410600142.647831putative ABC transporter ATP-binding protein
KPHS_410501171.220591putative ABC transporter permease
KPHS_410402210.235788putative periplasmic solute binding protein
KPHS_41030322-0.239331competence damage-inducible protein A
KPHS_41020423-1.017442recombinase A
KPHS_41010219-0.375781recombination regulator RecX
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_41110DHBDHDRGNASE871e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 87.4 bits (216), Expect = 1e-22
Identities = 68/256 (26%), Positives = 122/256 (47%), Gaps = 5/256 (1%)

Query: 3 QVAVVIGGGQTLGEFLCRGLAAEGYRVAVVDIQSEKASRVAQEINAEYGEGMAYGFGADA 62
++A + G Q +GE + R LA++G +A VD EK +V + AE A F AD
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE--ARHAEAFPADV 66

Query: 63 TSEASVTALAHGVDEIFSRVDLLVYSAGIAKAAFISDFALGDFDRSLQVNLVGYFLCARE 122
A++ + ++ +D+LV AG+ + I + +++ + VN G F +R
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 123 FSRLMIRDGIKGRIIQINSKSGKVGSKHNSGYSAAKFGGVGLTQSLALDLAEYGITVHSL 182
S+ M D G I+ + S V + Y+++K V T+ L L+LAEY I + +
Sbjct: 127 VSKYM-MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 183 MLGNLLKSPMFQSLLPQYATKLGIDESEVEQYYIDKVPLKRGCEYQDVLNVLMFYASPQA 242
G+ ++ M SL + + +E + +PLK+ + D+ + ++F S QA
Sbjct: 186 SPGST-ETDMQWSLWADENGAEQVIKGSLETFKTG-IPLKKLAKPSDIADAVLFLVSGQA 243

Query: 243 SYCTGQSINVTGGQVM 258
+ T ++ V GG +
Sbjct: 244 GHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_41050TYPE3IMSPROT280.038 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 28.2 bits (63), Expect = 0.038
Identities = 14/74 (18%), Positives = 27/74 (36%), Gaps = 3/74 (4%)

Query: 19 ALVVCLALSLSTTMLGVFLLLRRMSLMGDALSHAILP-GVAVGYLLSGMSLLAMTLGG-- 75
+ + +ALS L + LM + LP A+ Y++ + L L
Sbjct: 31 STALIVALSAMLMGLSDYYFEHFSKLMLIPAEQSYLPFSQALSYVVDNVLLEFFYLCFPL 90

Query: 76 FIAGIVVALVAGWV 89
++A+ + V
Sbjct: 91 LTVAALMAIASHVV 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_41040adhesinb2364e-79 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 236 bits (604), Expect = 4e-79
Identities = 92/308 (29%), Positives = 169/308 (54%), Gaps = 17/308 (5%)

Query: 1 MKRSAIVVALALGLMAQGAMAKT----------LNVVSSFSVLGDIAQQVGGEHVHVDTL 50
MK+ +V L L + A + LNVV++ S++ DI + + G+ +++ ++
Sbjct: 1 MKKCRFLVLLLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSI 60

Query: 51 VGPDGDPHTFEPSPKDSALLSKADVVVVNGLGLE----GWLDRLIKASGFKGE--LVVAS 104
V DPH +EP P+D S+AD++ NG+ LE W +L++ + K S
Sbjct: 61 VPVGQDPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVENAKKKENKDYYAVS 120

Query: 105 KGVKTHTLDEEGKTVT-DPHAWNSAANGALYAQNILDGLVKADPEDKAALTSSGKRYIDQ 163
+GV L+ + + DPHAW + NG +YAQNI L + DP +K + K Y+++
Sbjct: 121 EGVDVIYLEGQSEKGKEDPHAWLNLENGIIYAQNIAKRLSEKDPANKETYEKNLKAYVEK 180

Query: 164 LTSLDGWAKAQFSAIPLAKRKVLTSHDAFGYFGRAYHVTFLAPQGLSSESEASTAQVAAL 223
L++LD AK +F+ IP K+ ++TS F YF +AY+V +++E E + Q+ L
Sbjct: 181 LSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTL 240

Query: 224 IKQIKADGVHTWFMENQLDPRLVKQIASATGAQPGGELYPEALSKPGGVADSYVKMMRHN 283
+++++ V + F+E+ +D R +K ++ T +++ +++++ G DSY MM++N
Sbjct: 241 VEKLRKTKVPSLFVESSVDDRPMKTVSKDTNIPIYAKIFTDSVAEKGEEGDSYYSMMKYN 300

Query: 284 VELIAKSM 291
+E IA+ +
Sbjct: 301 LEKIAEGL 308


20KPHS_40790KPHS_40030Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_40790-2143.248393ribonucleotide-diphosphate reductase subunit
KPHS_407801212.229473ribonucleotide reductase stimulatory protein
KPHS_407700191.799134glutaredoxin protein
KPHS_407601210.306777hypothetical protein
KPHS_40750122-1.037043GntR family transcriptional regulator
KPHS_40740126-6.193546hypothetical protein
KPHS_40730123-6.514892hypothetical protein
KPHS_40720119-3.549398hypothetical protein
KPHS_40710221-2.972447hypothetical protein
KPHS_40700118-0.291403DNA-bending protein with chaperone activity
KPHS_40690-1202.661809hypothetical protein
KPHS_406800174.292377hypothetical protein
KPHS_406700165.529365putative phosphatase
KPHS_406600154.602403hypothetical protein
KPHS_406500143.748044acyl carrier protein phosphodiesterase
KPHS_406401143.391846LysR family transcriptional regulator
KPHS_406301141.834208putative Fis-type transcriptional regulator
KPHS_40620120-2.636506lactaldehyde dehydrogenase
KPHS_40610318-0.158368SsrA-binding protein
KPHS_40600315-0.040072polyketide cyclase/dehydrase family protein
KPHS_405903160.173767hypothetical protein
KPHS_40580314-0.545803hypothetical protein
KPHS_40570213-0.998322hypothetical protein
KPHS_40560016-6.452689DNA repair protein
KPHS_40550021-9.871697inorganic polyphosphate/ATP-NAD kinase
KPHS_40540021-10.174623Hsp 24 nucleotide exchange factor
KPHS_40530016-8.085085hypothetical protein
KPHS_40520120-8.599253hypothetical protein
KPHS_40510019-8.033828transposase
KPHS_40500-116-2.436014hypothetical protein
KPHS_40490120-3.119507hypothetical protein
KPHS_40480320-4.035496fifty-four-like protein
KPHS_40470231-6.00638530S ribosomal protein S16
KPHS_40460032-5.17270716S rRNA-processing protein
KPHS_40450032-4.858516tRNA (guanine-N(1)-)-methyltransferase
KPHS_40440133-4.95326550S ribosomal protein L19
KPHS_40430028-4.172230putative prophage tail protein
KPHS_40420022-2.926843putative prophage tail protein
KPHS_40410021-2.596101putative prophage tail length determinator
KPHS_40400317-2.083144putative prophage tail protein
KPHS_40390219-1.961039putative prophage tail protein
KPHS_40380219-1.988919putative prophage tail tube protein
KPHS_40370220-1.671013putative prophage tail sheath
KPHS_40360321-1.837895hypothetical protein
KPHS_40350319-1.374770hypothetical protein
KPHS_40340220-0.382920hypothetical protein
KPHS_40330318-0.536216tail protein I
KPHS_40320217-0.273576putative prophage baseplate assembly protein
KPHS_40310418-0.847002putative prophage baseplate protein
KPHS_40300320-0.715541putative prophage baseplate assembly protein
KPHS_40290325-1.998019putative prophage, tail completion protein
KPHS_40280226-1.469454putative prophage, tail completion protein
KPHS_40270226-0.383556hypothetical protein
KPHS_402605220.605114putative prophage P2 LysB-like protein
KPHS_402503200.102862putative prophage endolysin, control of lysis
KPHS_40240518-0.900868putative prophage holin
KPHS_40230516-1.575602putative prophage tail component protein
KPHS_40220616-1.362503putative prophage phage head completion protein
KPHS_40210616-1.775845putative prophage small terminase subunit
KPHS_40200416-5.361324putative prophage major capsid protein
KPHS_40190419-6.678719putative prophage major capsid protein
KPHS_40180321-7.325085putative prophage capsid scaffolding protein
KPHS_40170226-8.342311putative prophage large terminase protein
KPHS_40160134-8.828196putative prophage presumed portal protein
KPHS_40150036-8.605311hypothetical protein
KPHS_40140130-6.264017phage protein
KPHS_40130031-5.954626bacteriophage sos operon Tum protein
KPHS_40120-130-5.768938TumA
KPHS_40110-229-5.526201putative reverse transcriptase/maturase
KPHS_40100136-8.441940gp36
KPHS_40090549-12.563203hypothetical protein
KPHS_40080545-10.451379putative exonuclease CP81
KPHS_40070736-7.326007hypothetical protein
KPHS_40060438-7.974956hypothetical protein
KPHS_40050137-7.968009hypothetical protein
KPHS_40040035-7.224746hypothetical protein
KPHS_40030030-5.564857gp31
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_40630HTHFIS2871e-92 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 287 bits (737), Expect = 1e-92
Identities = 122/366 (33%), Positives = 173/366 (47%), Gaps = 52/366 (14%)

Query: 268 LTTPQGRYHYRLREPTRRRVAVSAPPAMHLPFTSPREGEKLLRLLNAGIALCIEGETGSG 327
L P+ R + V AM + L RL+ + L I GE+G+G
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIY------RVLARLMQTDLTLMITGESGTG 172

Query: 328 KEYVSRTLHRHSRWRSGKFVAINCAAIPESLIESELFGYQPGAFTGASKNGYIGKIREAD 387
KE V+R LH + + R+G FVAIN AAIP LIESELFG++ GAFTGA G+ +A+
Sbjct: 173 KELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRS-TGRFEQAE 231

Query: 388 GGVLFLDEIGDMPLALQTRLLRVLQEKEVAPLGASRSVPVNFALICATHRNLTQRVSAGE 447
GG LFLDEIGDMP+ QTRLLRVLQ+ E +G + + ++ AT+++L Q ++ G
Sbjct: 232 GGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGL 291

Query: 448 FREDLLWRLREYALALPPLREW----PALETFIATLWHDLGGASRRVTLSNALLVHLSQL 503
FREDL +RL L LPPLR+ P L G +R L +
Sbjct: 292 FREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKR--FDQEALELMKAH 349

Query: 504 PWPGNVRQLQSVLKVMLALADEGDTLTPDALPEAYRAAPAPLPRGG-------------- 549
PWPGNVR+L+++++ + AL D +T + + R+ P
Sbjct: 350 PWPGNVRELENLVRRLTALY-PQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAV 408

Query: 550 ------------------------LQAHDEQLIVDTLARVNGNVSRAAQILGIARSTLYR 585
L + LI+ L GN +AA +LG+ R+TL +
Sbjct: 409 EENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRK 468

Query: 586 RAARAG 591
+ G
Sbjct: 469 KIRELG 474


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_40600FLGMOTORFLIM280.017 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 27.6 bits (61), Expect = 0.017
Identities = 18/78 (23%), Positives = 34/78 (43%), Gaps = 8/78 (10%)

Query: 36 GSRVLELGPTQMTAAVDVSKAGISKTFTTRNTLTSNQSILMSLVDGPFKKLIGGWK---- 91
G+ VLE+ P+ + +D G + + LT I S+++G +++ +
Sbjct: 113 GNAVLEVDPSITFSIIDRLFGGTGQAAKVQRDLT---DIENSVMEGVIVRILANVRESWT 169

Query: 92 -FIPLSPEACKIEFHLDF 108
I L P +IE + F
Sbjct: 170 QVIDLRPRLGQIETNPQF 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_40430TONBPROTEIN290.028 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 28.8 bits (64), Expect = 0.028
Identities = 13/46 (28%), Positives = 21/46 (45%), Gaps = 5/46 (10%)

Query: 236 DPKPQKQKVTLKRKPKEQHLRALQHPKAKPVTKKKTVKEPEAREGE 281
P+P K+ + KPK + PK KPV K + + + + E
Sbjct: 78 IPEPPKEAPVVIEKPKPK-----PKPKPKPVKKVQEQPKRDVKPVE 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_40410GPOSANCHOR310.018 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 31.2 bits (70), Expect = 0.018
Identities = 30/175 (17%), Positives = 57/175 (32%), Gaps = 5/175 (2%)

Query: 6 KLQVLLKAVDQATRPFKSIQTASKTLSGDIRDTQKSLRELNGQAS-RIDGFRKASAQLAV 64
A + ++ + A D+ + + S +I A L
Sbjct: 201 GAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEA 260

Query: 65 TGQELKKAKQEAAALAIQFRNTEQPTRAQAQAMDA----ARKSAAALQLKHNSLRQAVQR 120
EL+KA + A + + A+ A++A + L SLR+ +
Sbjct: 261 RQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDA 320

Query: 121 QRQELSQAGINTRSLAADERRLKTSISETTAQLNRQREALARVSAQQAKLNAVKQ 175
R+ Q + L + + S L+ REA ++ A+ KL +
Sbjct: 321 SREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_40280SALSPVBPROT327e-04 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 32.0 bits (72), Expect = 7e-04
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 7/48 (14%)

Query: 105 SISLLLTERTLVKEADGAMY--VENIPEPPPPEPV-----TRPVEMWS 145
++ L RTL E DG V N+ PPPP P+ +RP W+
Sbjct: 340 ILTQLCAARTLAYEGDGYRRAPVNNMMPPPPPPPMMGGNSSRPKSKWA 387


21KPHS_39260KPHS_39200Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_39260-1124.138542aminopeptidase B
KPHS_39250-1124.096307enhanced serine sensitivity
KPHS_39240-1113.929451hypothetical protein
KPHS_39230-1114.0811953-mercaptopyruvate sulfurtransferase
KPHS_39220-1123.375537hypothetical protein
KPHS_392100112.365842penicillin binding protein 1C
KPHS_39200318-0.492412nucleoside diphosphate kinase
22KPHS_38810KPHS_38550Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_38810-3133.421973hypothetical protein
KPHS_38800-2153.207352putative acyl-CoA N-acyltransferase
KPHS_38790-1131.537828putative acyl-CoA N-acyltransferase
KPHS_38780-1132.230555hypothetical protein
KPHS_38770-1142.288212hypothetical protein
KPHS_38760-1142.558493succinyl-diaminopimelate desuccinylase
KPHS_387500142.919510hypothetical protein
KPHS_38740-1153.241875aminoglycoside/multidrug efflux system
KPHS_38730-1153.374798glutamate synthase subunit small chain
KPHS_38720-1152.049056MutT-like protein
KPHS_387100152.131329hypothetical protein
KPHS_387001131.828365hypothetical protein
KPHS_386901141.636913transketolase
KPHS_386801151.510947transaldolase A
KPHS_386701152.881955malic enzyme
KPHS_386601163.642417putative carboxysome structural protein in
KPHS_386500154.227144putative ethanolamine utilization protein
KPHS_386401175.320550putative regulator of ethanolamine utilization
KPHS_386302165.558755putative cobalamin adenosyltransferase in
KPHS_386203156.044607phosphotransacetylase
KPHS_386103144.276795detox protein
KPHS_386002144.745755detox protein
KPHS_385900154.009785ethanolamine utilization protein
KPHS_385800153.890262ethanolamine utilization protein
KPHS_385700143.601870ethanolamine utilization enzyme
KPHS_38560-1143.203031ethanolamine utilization, putative transport
KPHS_38550-2133.344377reactivating factor for ethanolamine ammonia
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_38740ACRIFLAVINRP12860.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1286 bits (3330), Expect = 0.0
Identities = 657/1032 (63%), Positives = 801/1032 (77%), Gaps = 2/1032 (0%)

Query: 1 MANFFIDRPIFAWVLAILLCLTGALAILSLPVEQYPDLAPPNVRITANYPGASAQTLENT 60
MANFFI RPIFAWVLAI+L + GALAIL LPV QYP +APP V ++ANYPGA AQT+++T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMTGLDNLMYMSSQSSATGQATITLSFTAGTDPDEAVQQVQNQLQSAMRKLPQ 120
VTQVIEQNM G+DNLMYMSS S + G TITL+F +GTDPD A QVQN+LQ A LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 AVQNQGVTVRKTGDTNILTLAFVSTDGSMDKQDIADYVASNIQDPLSRVNGVGDIDAYGS 180
VQ QG++V K+ + ++ FVS + + DI+DYVASN++D LSR+NGVGD+ +G+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYSMRIWLDPAKLNSYQMTTKDVTDAISSQNAQIAVGQLGGTPSVDKQALNATINSQSLL 240
QY+MRIWLD LN Y++T DV + + QN QIA GQLGGTP++ Q LNA+I +Q+
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 QTPEQFRNITLRVNQDGSEVTLGDVATVEMGAEKYDYLSRYNRQPASGLGIKLASGANEM 300
+ PE+F +TLRVN DGS V L DVA VE+G E Y+ ++R N +PA+GLGIKLA+GAN +
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 ATAERVINRLNELAQFFPHGLEYKVAYETTSFVKASITDVVKTLLEAILLVFLVMYLFLQ 360
TA+ + +L EL FFP G++ Y+TT FV+ SI +VVKTL EAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLMGTFAVLYACGYSINTLTMFAMVLAIGLLVDDAIVVVENVERIM 420
N RATLIPTIAVPVVL+GTFA+L A GYSINTLTMF MVLAIGLLVDDAIVVVENVER+M
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 SEEGLSPREATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGTTGAIYRQFSITIVAAMVL 480
E+ L P+EAT KSM QIQGALVGIAMVLSAVF+PMAFFGG+TGAIYRQFSITIV+AM L
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVAMILTPALCATLLKPVKPGESHERTGFFGWFNRTFNRSASRYETFVGKILHRSLRW 540
SVLVA+ILTPALCATLLKPV + GFFGWFN TF+ S + Y VGKIL + R+
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 541 MLIYVLLLGGMVFLFLHLPTSFLPLEDRGMFTTSVQLPSGSTQQQTLKVVQKAEDYFLNN 600
+LIY L++ GMV LFL LP+SFLP ED+G+F T +QLP+G+TQ++T KV+ + DY+L N
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 601 EKQNVESVFATVGSGPGGNGQNVARMFVRLKDWDQRDPQTGTSFAIIERATKAFNQINEA 660
EK NVESVF G G QN FV LK W++R+ ++ A+I RA +I +
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 661 RVIASSPPAISGLGSSAGFDMELEDHAGKGHDALMAARDTLLELAGKNPL-LTRVRHNGL 719
VI + PAI LG++ GFD EL D AG GHDAL AR+ LL +A ++P L VR NGL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 720 DDSPQLQVDIDQRKAQALGVSIDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAKYRM 779
+D+ Q ++++DQ KAQALGVS+ DIN T+ TA G +YVNDF+DRGRVKK+YVQA AK+RM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 780 LPDDINLWYVRNSSGTMVPFSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGISTGTAM 839
LP+D++ YVR+++G MVPFSAF TS W GSPRLERYNG ++EI GEAAPG S+G AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 840 DMMEKLAAQLPTGFGLEWTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSV 899
+ME LA++LP G G +WT MSYQERLSG QAPAL AIS +VVFLCLAALYESWS+P SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 900 MLVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANELNEK-GQDLL 958
MLVVPLG++G LLA + +NDVYF VGLLT IGLSAKNAILIVEFA +L EK G+ ++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 959 SATLSACRQRLRPILMTSLAFIFGVLPMATSTGAGSGSQHAVGTGVMGGMISATVLAIFF 1018
ATL A R RLRPILMTSLAFI GVLP+A S GAGSG+Q+AVG GVMGGM+SAT+LAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1019 VPLFFVLVRRRF 1030
VP+FFV++RR F
Sbjct: 1021 VPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_38580SHAPEPROTEIN435e-07 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 43.2 bits (102), Expect = 5e-07
Identities = 47/179 (26%), Positives = 69/179 (38%), Gaps = 41/179 (22%)

Query: 33 LGIDLGTCD----------------VVSMVVDRDGQPVAVCL--DWADVVL--------- 65
L IDLGT + VV++ DR G P +V A +L
Sbjct: 13 LSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAAVGHDAKQMLGRTPGNIAA 72

Query: 66 -----DGIVWDFFGAVTLVRRHLATLEQQLGCRFT-HAATSFPPGTDP---RISINVLES 116
DG++ DFF +++ + + R + P G R +
Sbjct: 73 IRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQG 132

Query: 117 AGLEISHVLDEPTAVA---DLLQLDNAG--VVDIGGGTTGIAIVKQGRVTYSADEATGG 170
AG +++EP A A L + G VVDIGGGTT +A++ V YS+ GG
Sbjct: 133 AGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGG 191


23KPHS_38220KPHS_38010Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_38220-215-3.524640bile acid/Na+ symporter family transporter
KPHS_38210-217-4.505182putative LysR-family transcriptional regulator
KPHS_38200-315-4.421887****glutamyl-tRNA synthetase
KPHS_38190-217-4.455490putative negative regulator
KPHS_38180-216-4.153082putative negative regulator
KPHS_38170-214-2.562382**hypothetical protein
KPHS_38160-1110.525555nucleoside permease
KPHS_381500111.763926manganese transport protein MntH
KPHS_38140-1110.763788hypothetical protein
KPHS_38130-111-0.087244putative oxidoreductase
KPHS_38120-111-0.124384putative pyruvate decarboxylase
KPHS_38110012-1.976662hypothetical protein
KPHS_38100012-3.566042glucokinase
KPHS_38090-112-2.034558putative 2-component transcriptional regulator
KPHS_38080014-0.830682putative sensor protein
KPHS_380702170.386411hypothetical protein
KPHS_38060218-1.372471phytanoyl-CoA dioxygenase
KPHS_38050217-0.712886aminotransferase
KPHS_38040320-0.768648putative cyanate transporter
KPHS_38030024-3.565446putative adenosine deaminase
KPHS_38020021-3.661997putative LysR family regulatory protein
KPHS_38010119-4.569192hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_38090HTHFIS556e-11 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 55.2 bits (133), Expect = 6e-11
Identities = 23/142 (16%), Positives = 58/142 (40%), Gaps = 9/142 (6%)

Query: 7 KVIIVEDEFLAQQELSWLINTHSQMEIVGSFDDGLDVLKFLQHNKVDAIFLDINIPSLDG 66
+++ +D+ + L+ ++ V + + +++ D + D+ +P +
Sbjct: 5 TILVADDDAAIRTVLNQALS--RAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 67 V-LLAQNISQFAHKPFIVFITAWK--EHAVEAFELEAFDYILKPYQESRIINMLQKLTTA 123
LL + P +V ++A A++A E A+DY+ KP+ + +I ++ + A
Sbjct: 63 FDLLPRIKKARPDLPVLV-MSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR---A 118

Query: 124 WQQQNNAASGLASAAPRENDTI 145
+ S L + +
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLV 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_38080PF065802174e-68 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 217 bits (555), Expect = 4e-68
Identities = 58/207 (28%), Positives = 101/207 (48%), Gaps = 11/207 (5%)

Query: 342 RAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE 401
++ MA +A+L AL+++INPHF+FNALN I + I +P AR+++ +LS +RY++
Sbjct: 150 DQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKAREMLTSLSELMRYSLR 209

Query: 402 LKDDEQIDIKRELYQIKDYIAIEQARFGDKLTVIYDIDDDV-SCVIPSLLIQPLVENAIV 460
+ Q+ + EL + Y+ + +F D+L I+ + +P +L+Q LVEN I
Sbjct: 210 YSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIK 269

Query: 461 HGIQPCKGKGVVTIGINECGNRVRISVRDTGNGIDPAVVARVEADEMPGNKIGLLNVHHR 520
HGI G + + + V + V +TG+ + GL NV R
Sbjct: 270 HGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALK--------NTKESTGTGLQNVRER 321

Query: 521 VKLLYGE--GLHIRNLTPGTEIAFYVP 545
+++LYG + + +P
Sbjct: 322 LQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_38040TCRTETA330.001 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 33.3 bits (76), Expect = 0.001
Identities = 73/361 (20%), Positives = 117/361 (32%), Gaps = 19/361 (5%)

Query: 31 LLPDIRAASGMSYTLAALLTALPVIAMGVLALAAGWVDRYIGQKRSIALSLLIIAAGALL 90
LL D+ ++ ++ LL ++ + DR+ G++ + +SL A +
Sbjct: 31 LLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRF-GRRPVLLVSLAGAAVDYAI 89

Query: 91 REIAPNSGLLLTSALAGGIGIGIIQAAIPAVIKHLFPRRT-PLVMGLWSAALMGGGGLGA 149
AP +L + GI G A A I + G SA G G
Sbjct: 90 MATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGP 148

Query: 150 AFTPWLA--SHSAAWHDALAWWALPALLALL----SWLAICRHLPRAPHQTSASSRVAII 203
+ S A + A A L L S R L R AS R A
Sbjct: 149 VLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARG 208

Query: 204 GQRRAWTLGLYFG--LINAGYASLIAWLPPYYIQLGDSAQYSGSLLALLTVGQTAGALLL 261
A + ++F L+ A+L D+ SL A + A A++
Sbjct: 209 MTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHW-DATTIGISLAAFGILHSLAQAMIT 267

Query: 262 PALARQEDRRQLLLLALALQLIGFCGFIWLPEHFSALWAIACGVGLGGAFPLC---LVLA 318
+A + R+ L+L + G+ + + A + G P L
Sbjct: 268 GPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQ 327

Query: 319 LDHAGQPAVAGRLVAFMQGIGFIIAGLSPWLSGLLRSLSGNYTLDWSWHAICVLLLMALT 378
+D Q + G L A + P L + + S W+W A L L+ L
Sbjct: 328 VDEERQGQLQGSLAALTSLTSIV----GPLLFTAIYAASITTWNGWAWIAGAALYLLCLP 383

Query: 379 L 379

Sbjct: 384 A 384


24KPHS_37340KPHS_37290Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_373401133.328069NADH dehydrogenase subunit M
KPHS_373301134.451699NADH dehydrogenase subunit N
KPHS_373200134.359589ribonuclease Z
KPHS_373101144.986517putative transferase
KPHS_373001145.114816menaquinone-specific isochorismate synthase
KPHS_372900134.3995092-oxoglutarate decarboxylase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_37310AUTOINDCRSYN341e-04 Autoinducer synthesis protein signature.
		>AUTOINDCRSYN#Autoinducer synthesis protein signature.

Length = 216

Score = 34.1 bits (78), Expect = 1e-04
Identities = 14/74 (18%), Positives = 28/74 (37%), Gaps = 12/74 (16%)

Query: 1 MMTWQDLHHSELTVPQLYALLKLRSEVFV--------VEQQCVYQDVDGDDLVGENRHLL 52
M+ D++H+ L+ + L LR E F + D ++ +L
Sbjct: 1 MLEIFDVNHTLLSETKSGELFTLRKETFKDRLNWAVQCTDGMEFDQYDNNN----TTYLF 56

Query: 53 GWRDGELVAYARIL 66
G +D ++ R +
Sbjct: 57 GIKDNTVICSLRFI 70


25KPHS_37190KPHS_37090Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_37190-2113.052627putative regulator
KPHS_37180-2123.985657putative dehydratase
KPHS_37170-2103.435309major facilitator family transporter
KPHS_37160-292.253678putative aldolase
KPHS_37150-2101.990411sn-glycerol-3-phosphate dehydrogenase subunit C
KPHS_37140-2131.259131anaerobic glycerol-3-phosphate dehydrogenase
KPHS_37130-117-0.406826sn-glycerol-3-phosphate dehydrogenase large
KPHS_37120018-1.792018sn-glycerol-3-phosphate transporter
KPHS_37110219-1.354638glycerophosphoryl diester phosphodiesterase
KPHS_37100216-0.9580252Fe-2S iron-sulfur cluster-binding protein
KPHS_37090216-1.244178ribonucleotide-diphosphate reductase subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_37160PHPHTRNFRASE413e-06 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 41.3 bits (97), Expect = 3e-06
Identities = 28/171 (16%), Positives = 61/171 (35%), Gaps = 31/171 (18%)

Query: 94 LLVPMVDTAEQAREVVSATRYPPIGSRGVGAGVARAARWGRVENYMAEANDELCLLIQVE 153
++ PM+ T E+ R+ A + E + E+ +++++
Sbjct: 389 VMFPMIATLEELRQAK--------------AIMQEEKDKLLSEGVDVSDSIEVGIMVEIP 434

Query: 154 SRTALENLDAILEVDGIDGVFIGPADL----------SASLGYPDDAGHPDVQRVIEQSI 203
S + + +D IG DL + + Y HP + R+++ I
Sbjct: 435 S--TAVAANLFAKE--VDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPAILRLVDMVI 490

Query: 204 RRIRAAGKAAGF---LAVDPAMAEKCLAWGANFVAVGVDTMLYTQALDRRL 251
+ + GK G +A D L G + ++ ++L ++ +L
Sbjct: 491 KAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKL 541


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_37120TCRTETA290.029 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 29.4 bits (66), Expect = 0.029
Identities = 23/119 (19%), Positives = 46/119 (38%), Gaps = 6/119 (5%)

Query: 59 GFSRGDLGFALSGISIAYGFSK-FIMGSVSDRSNPRIFLPAGLILAALVMLVMGFVPWAT 117
+ +G +L+ I + ++ I G V+ R R L G+I +++ F
Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGW 301

Query: 118 SSIMIMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSVWNCAHNVGGGIPPLLF 176
+ IM +L G+G P + ++ +G + ++ + PLLF
Sbjct: 302 MAFPIMVLLASG-----GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLF 355


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_37110VACCYTOTOXIN290.042 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 28.8 bits (64), Expect = 0.042
Identities = 13/54 (24%), Positives = 23/54 (42%), Gaps = 4/54 (7%)

Query: 252 NYSYDWMFKPGAMAQIAQYADGIGPDYHMLVAEGSKPGAVKLTAMVKEAHASHL 305
+Y YD+ F A+ +G Y+ L + K + + A+ A + HL
Sbjct: 1143 SYGYDFAFFRNALVLKPS----VGVSYNHLGSTNFKSNSNQKVALKNGASSQHL 1192


26KPHS_36340KPHS_36110Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_36340-2163.661021acetyltransferase
KPHS_36330-1174.085424D-lactate dehydrogenase
KPHS_363200204.827089beta-D-glucoside glucohydrolase, periplasmic
KPHS_363103205.266174glycine betaine/choline ABC transporter membrane
KPHS_363004205.015024putative ABC-type proline/glycine betaine
KPHS_362903223.375350ATPase
KPHS_36280-1191.798967putative ABC-type proline/glycine betaine
KPHS_36270-1190.511604hypothetical protein
KPHS_362600190.899208sensor histidine kinase
KPHS_36250-1200.626062putative two-component response-regulatory
KPHS_36240-2201.014656hypothetical protein
KPHS_36230-2211.096330methionyl-tRNA synthetase
KPHS_36220-1142.091879hypothetical protein
KPHS_36210-1143.383445putative ATP-binding protein
KPHS_36200-1134.942703putative ATP-binding protein
KPHS_36190-1135.044245hypothetical protein
KPHS_36180-1134.777167hypothetical protein
KPHS_361700145.095494LysR family transcriptional regulator
KPHS_361600156.214613putative xanthine/uracil/vitamin C permease
KPHS_361501166.877639putative amidohydrolase
KPHS_361400184.900822LysR family transcriptional regulator
KPHS_361301162.915625universal stress protein A
KPHS_361200133.583115hypothetical protein
KPHS_36110-1133.647505hydroxyethylthiazole kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_36260PF065802111e-65 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 211 bits (539), Expect = 1e-65
Identities = 59/216 (27%), Positives = 117/216 (54%), Gaps = 3/216 (1%)

Query: 343 LGEGIAQLLSAQILAGQYERQKALLTQSEIKLLHAQVNPHFLFNALNTLKAVIRRDSDQA 402
L G + + + ++ ++++ L AQ+NPHF+FNALN ++A+I D +A
Sbjct: 134 LYFGWHFFKNYKQAEIDQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKA 193

Query: 403 GQLVQYLSTFFRKNLKR-PTEIVTLADEIEHVNAYLQIEKARFQANLQIQMAVPEGLAHH 461
+++ LS R +L+ V+LADE+ V++YLQ+ +F+ LQ + + +
Sbjct: 194 REMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDV 253

Query: 462 QLPAFTLQPIVENAIKHGTSQHLGVGEITIRASQDDRWLQLDIEDNAGL-YRANPQASGL 520
Q+P +Q +VEN IKHG +Q G+I ++ ++D+ + L++E+ L + +++G
Sbjct: 254 QVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGT 313

Query: 521 GMNLVDRRLRARFGADCGISVTCEPERFTRVTLRLP 556
G+ V RL+ +G + I ++ + + + +P
Sbjct: 314 GLQNVRERLQMLYGTEAQIKLSEKQGKVN-AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_36250HTHFIS713e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 70.6 bits (173), Expect = 3e-16
Identities = 36/168 (21%), Positives = 70/168 (41%), Gaps = 10/168 (5%)

Query: 3 RVLIVDDEPLARENLRILLETQRDIEIVGECGNAVEAIGAVHKLRPDVLFLDIQMPRISG 62
+L+ DD+ R L L + ++ NA + D++ D+ MP +
Sbjct: 5 TILVADDDAAIRTVLNQALS-RAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 63 LEMVGMLDPEHRPYI--VFLTAFD--EYAVKAFEEHAFDYLLKPIEAARLEKTLARLRQE 118
+++ + + RP + + ++A + A+KA E+ A+DYL KP + L + R E
Sbjct: 63 FDLLPRIK-KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 119 RNLQDVSLLDDAQQTLKYIPCTGHSRIWLLQMEDVAFVSSRMSGIYVT 166
+ L DD+Q + + G S +A + + +T
Sbjct: 122 PKRRPSKLEDDSQDGMPLV---GRSAAMQEIYRVLARLMQTDLTLMIT 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_36190SACTRNSFRASE341e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 33.8 bits (77), Expect = 1e-04
Identities = 15/85 (17%), Positives = 35/85 (41%), Gaps = 7/85 (8%)

Query: 58 DEQLWVAECDGQPVGFAAV---WTADNFLHHLFVDPDWQGKHIGSALLAQVERTFTASGT 114
+ ++ + +G + W + + V D++ K +G+ALL + +
Sbjct: 64 GKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHF 123

Query: 115 LKCLMENKN----ALRFYQRHGWTI 135
++E ++ A FY +H + I
Sbjct: 124 CGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_36150UREASE340.001 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 34.3 bits (79), Expect = 0.001
Identities = 27/105 (25%), Positives = 46/105 (43%), Gaps = 17/105 (16%)

Query: 13 QAARGESPFDLLLIDAQIVDMATGEIRPADVGIVGEMIASVHPRGSRE----------DA 62
Q R D ++ +A I+D G I AD+G+ IA++ G+ +
Sbjct: 60 QVTREGGAVDTVITNALILD-HWG-IVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPG 117

Query: 63 HEVRSLAGGYLSPGLMDTHVHLESSHLPPERYAEIVLTQGTTAVF 107
EV + G ++ G MD+H+H + P++ E L G T +
Sbjct: 118 TEVIAGEGKIVTAGGMDSHIHF----ICPQQ-IEEALMSGLTCML 157


27KPHS_35010KPHS_34730Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_35010226-6.451809hypothetical protein
KPHS_35000541-13.606554putative dehydrogenase
KPHS_34990446-15.560908putative regulatory protein TetR
KPHS_34980348-15.284916putative sodium:hydrogen antiporter
KPHS_34970358-18.967132hypothetical protein
KPHS_34960355-17.200298putative Retron-type reverse transcriptase
KPHS_34950252-14.255526hypothetical protein
KPHS_34940544-8.132065hypothetical protein
KPHS_34930539-7.711499hypothetical protein
KPHS_34920233-8.616225hypothetical protein
KPHS_34910234-8.710877hypothetical protein
KPHS_34900233-9.802481hypothetical protein
KPHS_34890230-8.307382putative Antirestriction protein ardC
KPHS_34880328-7.616219putative MobC mobilization protein
KPHS_34870428-6.985219putative MobB mobilization protein
KPHS_34860324-5.521764hypothetical protein
KPHS_34850329-6.539990type IV secretory pathway VirB11 component
KPHS_34840231-5.894048type IV secretory pathway VirB10 component
KPHS_34830129-6.724310type IV secretory pathway VirB9 component
KPHS_34820130-7.261717type IV secretion system VirB8 component
KPHS_34810031-6.807456hypothetical protein
KPHS_34800131-6.826608hypothetical protein
KPHS_34790126-6.006096hypothetical protein
KPHS_34780228-5.999317putative type IV secretory pathway VirB4
KPHS_34770449-10.533306hypothetical protein
KPHS_34760235-7.102252putative type IV secretory pathway VirB1
KPHS_34750231-5.782320hypothetical protein
KPHS_34740031-5.119141hypothetical protein
KPHS_34730026-3.826058hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35000NUCEPIMERASE320.002 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 32.1 bits (73), Expect = 0.002
Identities = 13/52 (25%), Positives = 22/52 (42%)

Query: 92 ILVTGASGGVGSTAVVLLKALGYRVTAVSGRESTHDYLRQLGADTILPRSDF 143
LVTGA+G +G L G++V + +D + +L + F
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGF 54


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_34990HTHTETR716e-17 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 71.2 bits (174), Expect = 6e-17
Identities = 26/167 (15%), Positives = 58/167 (34%), Gaps = 3/167 (1%)

Query: 16 PPKVDRQFDDTRQALIRSGLEVLTETGYLAAGIDAVIKNIAVPKGSFYHCFKSKEAFGLA 75
K ++ +TRQ ++ L + ++ G + + + K V +G+ Y FK K
Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 76 VLAAYGDFFAHKLDKFLLDDAVPPLERMAAFVRHAGQGMEKFQFRRGCLVGNLLQEAPLL 135
+ ++ PL + + H + + RR L+ + + +
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRL-LMEIIFHKCEFV 120

Query: 136 PETFPQRLMAILAAWESR--VARCLREAQAAGAIASDASPQALAQVF 180
E + ES + + L+ A + +D + A +
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_34930GPOSANCHOR357e-04 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 35.4 bits (81), Expect = 7e-04
Identities = 19/91 (20%), Positives = 46/91 (50%), Gaps = 2/91 (2%)

Query: 223 LDQLLEDYGDRLKELTKSPKELKEQLEKINTSLRQQAAQVNTTEDEFQEAAGKRRELRKK 282
L+ D + + L + + L+ L+ + +Q A+ E++ + + R+ LR+
Sbjct: 293 LEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRD 352

Query: 283 LEESRERRAEVSAMLERFRLLDRHYLSDIER 313
L+ SRE + ++ A + +L +++ +S+ R
Sbjct: 353 LDASREAKKQLEAEHQ--KLEEQNKISEASR 381



Score = 34.7 bits (79), Expect = 0.001
Identities = 38/276 (13%), Positives = 87/276 (31%), Gaps = 31/276 (11%)

Query: 206 SNKSEPEELSRKAQLRLLDQLLEDYGDRLKELTKSPKEL----------KEQLEKINTSL 255
+ K + + + L +++ R +L K+ + + LE +L
Sbjct: 96 NAKEKLRKNDKS--LSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 153

Query: 256 RQQAAQVNTTEDEFQEAAGKRRELRKKLEESRER-RAEVSAMLERFRLLDRHYLSDIERL 314
+ A + + + K LE + A + + + +D ++
Sbjct: 154 AARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKI 213

Query: 315 RAIEEGGTLFSVLSAGHCPLCGAAPDHHPPDTGCNGDTDAVVQAARVEIAKIEVLRTELV 374
+ +E + A A + + A ++ E A +E + EL
Sbjct: 214 KTLEAEKAALAARKADLEKALEGAMNF-------STADSAKIKTLEAEKAALEARQAELE 266

Query: 375 ATVQSLEREGANFDRRIPTVVRE---LESISESVEEFLAPKLSTLRKSYSDFADKRAEVR 431
++ +I T+ E LE+ +E + + D R +
Sbjct: 267 KALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKK 326

Query: 432 EALALYATVQ------DMERR--RADLEKGTEDEKA 459
+ A + ++ + R+ R DL+ E +K
Sbjct: 327 QLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQ 362



Score = 31.2 bits (70), Expect = 0.014
Identities = 26/112 (23%), Positives = 52/112 (46%), Gaps = 10/112 (8%)

Query: 197 ADDSALVATSNKSEPEELSRKAQLRLLD------QLLEDYGDRLKELT----KSPKELKE 246
A ++ ++S+ +R++ R LD + LE +L+E S + L+
Sbjct: 292 ALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRR 351

Query: 247 QLEKINTSLRQQAAQVNTTEDEFQEAAGKRRELRKKLEESRERRAEVSAMLE 298
L+ + +Q A+ E++ + + R+ LR+ L+ SRE + +V LE
Sbjct: 352 DLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALE 403


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_34830TYPE4SSCAGX551e-10 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 55.2 bits (132), Expect = 1e-10
Identities = 60/250 (24%), Positives = 94/250 (37%), Gaps = 53/250 (21%)

Query: 105 VVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSAQQPAFQVSYRYPGE-------ERDK 157
+ + P+ W TN++V T+K LY L + + V YP E +
Sbjct: 268 IELSPSDSAWRTNLVVRTNKALYQFILRIAQKDNFASAYLTVKLEYPQRHEVSSVIEEEL 327

Query: 158 ASREATARQREW------------------------------EQKQ----------QHAS 177
RE RQRE E+KQ +
Sbjct: 328 KKREEAKRQRELIKQENLNTTAYINRVMMASNEQIINKEKIREEKQKIILDQAKALETQY 387

Query: 178 VQKALNSAQTPRNWNYTKYPGKGSFYIDPDFAYDDGRFTFVGFSPSKSIPSV-TKELNGK 236
V AL PRN+NY + P K S +I P +DDG FT+ GF P++ + +GK
Sbjct: 388 VHNALKRNPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQPAIFVVQPDGK 447

Query: 237 EHVVNSSIQKKGNFTVL---VIQEVTPRLVLRSGYTVVGLENSGFGKVHAADGSTVSR-- 291
+ +++I + L + E+ + L +V + N G+GK +
Sbjct: 448 LSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVTVINKGYGKNPLTKNYNIKNYG 507

Query: 292 QVERVEKPEP 301
++ERV K P
Sbjct: 508 ELERVIKKLP 517



Score = 33.2 bits (75), Expect = 0.001
Identities = 30/173 (17%), Positives = 73/173 (42%), Gaps = 24/173 (13%)

Query: 38 YNPQNVTVVNTKPGFM---------------TTLVFDNDEAVISARPGFDEAWEATPDAN 82
+N V VVN K ++ T + + DE + GF++ W P++N
Sbjct: 34 FNRGRVKVVNKKIAYLGDEKPITIWTSLDNVTVIQLEKDETISYITTGFNKGWSIVPNSN 93

Query: 83 RVNVRPVALTQGAPGEDGNTTQVVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSAQQP 142
+ ++P ++ E ++ +RD+ + +K+L ++ D K ++
Sbjct: 94 HIFIQPKSVKSNLMFEKEAVNFALM---TRDYQE---FLKTKKLI---VDAPDPKELEEQ 144

Query: 143 AFQVSYRYPGEERDKASREATARQREWEQKQQHASVQKALNSAQTPRNWNYTK 195
+ +E+ + +++ +R+ E+ + A+++ N+ P+N + K
Sbjct: 145 KKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANLENLTNAMSNPQNLSNNK 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_34820PF043351732e-56 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 173 bits (439), Expect = 2e-56
Identities = 51/223 (22%), Positives = 97/223 (43%), Gaps = 11/223 (4%)

Query: 4 QKLIAESRTFEQQMIERDKRATKAGFVVGGVGLLIAVLALVAVVVMLPLKQTDVELYTVD 63
+ E+ ++E+ + +R+ K +VV GV +A +VAV + PLK + + TVD
Sbjct: 11 KAYFEEAASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVD 70

Query: 64 NHTGRVEHVTR-TSKTSLTATEAYQKAMAANYVKVRERYVYPSLQDDYETVQVYNAPQVN 122
+TG + ++T EA +K A YV+ RE ++ + ++ ++ V V +A
Sbjct: 71 RNTGEASIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQ 130

Query: 123 DDYLALYAGKN--APDKVYKNGAHTVKVEILSNQITDATAPDRVATIRYKKIIRRLADNS 180
D + Y N +P + N V VEI VA + + K ++++
Sbjct: 131 DRWSRFYKTDNPQSPQNILANRT-DVFVEIKRVSFL----GGNVAQVYFTKESVTGSNST 185

Query: 181 TRNEYWDARFTFHSDPDKEMSDAEREINYFGFTVTSWQTDREI 223
+ ++ + +R N G+ V S++ D E+
Sbjct: 186 KTDAVATIKYKV---DGTPSKEVDRFKNPLGYQVESYRADVEV 225


28KPHS_34290KPHS_34210Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_34290-116-3.272402hypothetical protein
KPHS_34280-114-2.035726tyrosine-specific transport protein
KPHS_34270017-3.603794hypothetical protein
KPHS_34260-113-3.403350ferritin
KPHS_34250012-3.565954hypothetical protein
KPHS_34240-111-2.758698hypothetical protein
KPHS_34230-113-3.019294MFS family transporter
KPHS_34220014-4.782719hypothetical protein
KPHS_34210-111-3.312724putative oligogalacturonide transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_34290SECA586e-12 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 57.6 bits (139), Expect = 6e-12
Identities = 23/51 (45%), Positives = 26/51 (50%)

Query: 164 ERITPAALALYQYWIANPQPVEAPQPIRNEAKVGRNDPCPCGSGKKYKQCC 214
E A + + + A E KVGRNDPCPCGSGKKYKQC
Sbjct: 847 EAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCH 897


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_34230TCRTETB1042e-26 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 104 bits (262), Expect = 2e-26
Identities = 81/395 (20%), Positives = 158/395 (40%), Gaps = 20/395 (5%)

Query: 25 FMEFLDGTVIATALPDMARDFGVTAVELNIGISAYLITLAVLIPASGWIADRFGARAIFT 84
F L+ V+ +LPD+A DF N +A+++T ++ G ++D+ G + +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 85 LALAIFTLASVFCGLS-TEVHIFVAMRILQGVGGALMVPVGRLAVLRTTPKHQLIKAIAT 143
+ I SV + + + + R +QG G A + + V R PK KA
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 144 LTWPALVAPIIGPPLGGFITRYASWHWIFFINVPLGLAAIILSLRIIPDIRETERRSFDL 203
+ + +GP +GG I Y HW + + +P+ + L + + FD+
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 204 SGFITTSVAMVSLVTAMERLGDRQPQIWPTLALAALGFGCLLYSIRHFRRAAAPMVRLDA 263
G I SV +V + L ++ L F ++H R+ P V
Sbjct: 202 KGIILMSVGIVFFMLFTTS------YSISFLIVSVLSFLIF---VKHIRKVTDPFVDPGL 252

Query: 264 LQVPTFRVTMYGGSLFRASISAVPFLLPLLFQVGFGMDPFHSGLLVLAVFVGNLTI---K 320
+ F + + G + +++ ++P + + + G +++ F G +++
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVII--FPGTMSVIIFG 310

Query: 321 PATTPLIRWLGFRRLLLINGALNVCSLLACALLTPQTPVW-AIMLILYLGGVFRSIQFTG 379
L+ G +L I S L + L T + I+++ LGG+ S T
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL--SFTKTV 368

Query: 380 VSTLAFADVPAAQMSDANTLFSTASQLAVGLGITL 414
+ST+ + + + +L + S L+ G GI +
Sbjct: 369 ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAI 403


29KPHS_33930KPHS_33850Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_33930213-1.602355high-affinity zinc uptake system periplasmic
KPHS_33920315-1.232878hypothetical protein
KPHS_33910314-1.199085lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
KPHS_33900216-0.884576pyruvate kinase
KPHS_33890112-1.107424transcriptional regulator HexR
KPHS_338801140.303648hypothetical protein
KPHS_338700141.815146glucose-6-phosphate 1-dehydrogenase
KPHS_33860-1133.135918phosphogluconate dehydratase
KPHS_33850-2123.143836phosphogluconate dehydratase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_33930ADHESNFAMILY2418e-81 Adhesin family signature.
		>ADHESNFAMILY#Adhesin family signature.

Length = 309

Score = 241 bits (617), Expect = 8e-81
Identities = 67/303 (22%), Positives = 117/303 (38%), Gaps = 27/303 (8%)

Query: 6 TLLCAGLGAVFLFAQVPLASAA-------VVTSMKPLGFIAAAIADGVTETQVLLPDGAS 58
TLL L A+ L A VV + + I IA + ++P G
Sbjct: 6 TLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIADITKNIAGDKIDLHSIVPIGQD 65

Query: 59 EHDYSLRPSDVKRLQNADLVVWIGPEMEAFMDKSTQSIAANKKVTIAELDGVKPLLITGA 118
H+Y P DVK+ ADL+ + G +E + + N K T + ++
Sbjct: 66 PHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTKLVENAKKT----ENKDYFAVSDG 121

Query: 119 DDDDDHHGHGHGAAEKGDGDHHHGIYNMHLWLSPEIARLSAVAIHDKLLELMPQSRAKLD 178
D G EKG D H WL+ E + A I +L P ++ +
Sbjct: 122 VDVIYLEGQN----EKGKEDPH-------AWLNLENGIIFAKNIAKQLSAKDPNNKEFYE 170

Query: 179 SNLQQFETALAATDKQVSNELA--PLKGKGYFVFHDAYGYFEKHYGLTSLGHFTVNPEIQ 236
NL+++ L DK+ ++ P + K A+ YF K YG+ S + +N E +
Sbjct: 171 KNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEE 230

Query: 237 PGAQRLHEIRTQLVEQKATCVFAEPQFRPAVIEAVARGTSVRMGT---LDPLGTGITLGK 293
+++ + +L + K +F E ++ V++ T++ + D + G
Sbjct: 231 GTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSIAEQGKEGD 290

Query: 294 TSY 296
+ Y
Sbjct: 291 SYY 293


30KPHS_33420KPHS_33090Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_33420-1153.677073hypothetical protein
KPHS_33410-1133.226535para-aminobenzoate synthase
KPHS_33400-1141.604898hypothetical protein
KPHS_33390-1132.117977endoribonuclease L-PSP
KPHS_33380-1142.016051putative ATP-dependent helicase
KPHS_33370-2110.347060putative glycoprotein endopeptidase
KPHS_33360-210-1.085304hypothetical protein
KPHS_33350-210-1.934985long-chain-fatty-acid--CoA ligase
KPHS_33340116-2.266156ribonuclease D
KPHS_33330120-4.302775cell division topological specificity factor
KPHS_33320-120-3.624650cell division inhibitor MinD
KPHS_33310-120-3.199742septum site-determining protein MinC
KPHS_33300318-2.276709hypothetical protein
KPHS_332902150.190332hypothetical protein
KPHS_332801140.229050hypothetical protein
KPHS_33270114-0.763098hypothetical protein
KPHS_33260013-1.102640hypothetical protein
KPHS_33250-1140.339814disulfide bond formation protein B
KPHS_33240-2161.565330sodium/proton antiporter
KPHS_33230-1172.060535fatty acid metabolism regulator
KPHS_33220-1192.762534hypothetical protein
KPHS_332101204.919697hypothetical protein
KPHS_332000214.910182D-amino acid dehydrogenase small subunit
KPHS_331901184.612252alanine racemase
KPHS_33180-1174.031767potassium/proton antiporter
KPHS_331701173.576191muramoyltetrapeptide carboxypeptidase
KPHS_331601173.185483hypothetical protein
KPHS_331501173.049778membrane-bound lytic murein transglycosylase E
KPHS_331401173.007829hypothetical protein
KPHS_33130-1130.476486periplasmic trehalase
KPHS_331201140.297229hypothetical protein
KPHS_331102140.911461hypothetical protein
KPHS_331003140.785086hypothetical protein
KPHS_33090216-0.518443hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_33190ALARACEMASE5390.0 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 539 bits (1391), Expect = 0.0
Identities = 286/356 (80%), Positives = 319/356 (89%)

Query: 1 MTRPVVASIDLLALRQNLQIVRRAAPGSRLWAVVKANAYGHGVARVWSALSAADGFALLN 60
MTRP+ AS+DL AL+QNL IVR+AA +R+W+VVKANAYGHG+ R+WSA+ A DGFALLN
Sbjct: 1 MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLN 60

Query: 61 LEEAILLREQGWKGPILLLEGFFHADELAVLDQYRLTTSVHSNWQIKALQQAKLRAPLDI 120
LEEAI LRE+GWKGPIL+LEGFFHA +L + DQ+RLTT VHSNWQ+KALQ A+L+APLDI
Sbjct: 61 LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI 120

Query: 121 YLKVNSGMNRLGFMPERVHTVWQQLRAISNVGEMTLMSHFAEAENPQGIVEPMRRIEQAA 180
YLKVNSGMNRLGF P+RV TVWQQLRA++NVGEMTLMSHFAEAE+P GI M RIEQAA
Sbjct: 121 YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA 180

Query: 181 EGLDCPRSLANSAATLWHPEAHFDWVRPGIVLYGASPSGQWQDIANTGLKPVMTLRSEII 240
EGL+C RSL+NSAATLWHPEAHFDWVRPGI+LYGASPSGQW+DIANTGL+PVMTL SEII
Sbjct: 181 EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII 240

Query: 241 GVQNLRPGEAIGYGGLYRTTQEQRIGIVACGYADGYPRVAPSGTPVLVDGVRTTTVGRVS 300
GVQ L+ GE +GYGG Y EQRIGIVA GYADGYPR AP+GTPVLVDGVRT TVG VS
Sbjct: 241 GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS 300

Query: 301 MDMLAVDLTPCPQAGIGAPVELWGKEIKIDDVAASSGTVGYELMCALAPRVPVVTL 356
MDMLAVDLTPCPQAGIG PVELWGKEIKIDDVAA++GTVGYELMCALA RVPVVT+
Sbjct: 301 MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV 356


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_33150VACJLIPOPROT270.043 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 27.2 bits (60), Expect = 0.043
Identities = 9/37 (24%), Positives = 14/37 (37%)

Query: 1 MKLRWLLILVVFLAGCSSKHDYTNPPWNPEVPVKRAM 37
++L L + L GC+S +P R M
Sbjct: 3 LRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTM 39


31KPHS_33000KPHS_32550Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_330000223.806867propionate kinase
KPHS_329900223.059593formate C-acetyltransferase
KPHS_329801233.100227putative formate acetyltransferase
KPHS_329701223.081356hypothetical protein
KPHS_329603243.686539high-affinity branched-chain amino acid ABC
KPHS_329502211.784803high-affinity branched-chain amino acid ABC
KPHS_32940-1151.795005high-affinity branched-chain amino acid
KPHS_329300151.562031high-affinity branched-chain amino acid
KPHS_329201151.857955ATP-binding component of high-affinity
KPHS_32910-1141.389075ATP-binding component of leucine transport
KPHS_32900-3131.056725LysR family transcriptional regulator
KPHS_32890-2153.249608phosphoenolpyruvate carboxykinase
KPHS_32880-1173.527753putative acetyltransferase
KPHS_32870-3172.481645transcriptional regulator LysR
KPHS_32860-2172.354799major facilitator superfamily permease
KPHS_32850-3171.858844major facilitator superfamily permease
KPHS_32840-2171.885610molybdate ABC transporter periplasmic
KPHS_32830017-0.978068LysR family transcriptional regulator
KPHS_32820-1130.358031cold shock protein E
KPHS_328100142.508463putative ribosomal large subunit pseudouridine
KPHS_32800-1133.631353putative nucleoprotein/polynucleotide-associated
KPHS_32790-1144.182855hypothetical protein
KPHS_32780-1161.957403hypothetical protein
KPHS_32770-1180.633313hypothetical protein
KPHS_32760024-1.906510membrane protein
KPHS_32750129-4.278311hypothetical protein
KPHS_32740242-9.356282hypothetical protein
KPHS_32730345-10.340677hypothetical protein
KPHS_32720767-15.332867hypothetical protein
KPHS_32710869-18.016230hypothetical protein
KPHS_32700866-17.191756hypothetical protein
KPHS_32690964-16.430554hypothetical protein
KPHS_32680965-16.317352hypothetical protein
KPHS_32670863-15.904085hypothetical protein
KPHS_32660654-14.326048hypothetical protein
KPHS_32650754-14.178368sulfatase modifying factor 1 -like protein
KPHS_32640651-13.411752hypothetical protein
KPHS_32630754-14.627505sulfatase modifying factor 1
KPHS_32620751-13.131189hypothetical protein
KPHS_32610750-12.605253transposase InsC for insertion sequence IS903
KPHS_325901064-17.400402hypothetical protein
KPHS_326001166-17.658468hypothetical protein
KPHS_325801070-18.621054sulfatase modifying factor 1
KPHS_32570865-15.492171sulfatase modifying factor 1
KPHS_32560343-9.122707hypothetical protein
KPHS_32550221-1.425990hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_33000ACETATEKNASE5020.0 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 502 bits (1295), Expect = 0.0
Identities = 163/395 (41%), Positives = 246/395 (62%), Gaps = 11/395 (2%)

Query: 7 VLVINCGSSSIKFSVLDAASCDCLLNGVAEGINAERASLSLNGGE---PVALAQRGYEGA 63
+LVINCGSSS+K+ ++++ + L G+AE I + L+ N + + ++ A
Sbjct: 3 ILVINCGSSSLKYQLIESKDGNVLAKGLAERIGINDSLLTHNANGEKIKIKKDMKDHKDA 62

Query: 64 LQAIAGALAQRDL-----IDSVALIGHRVAHGGDLFTESVIISEEVINNIRQVSSLAPLH 118
++ + AL D + + +GHRV HGG+ FT SV+I+++V+ I LAPLH
Sbjct: 63 IKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGEYFTSSVLITDDVLKAITDCIELAPLH 122

Query: 119 NYASLSGIASAQRLFPEVMQVAVFDTSFHQTLAPEAFLYGLPWEYYQNLGVRRYGFHGTS 178
N A++ GI + ++ P+V VAVFDT+FHQT+ A+LY +P+EYY +R+YGFHGTS
Sbjct: 123 NPANIEGIKACTQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRKYGFHGTS 182

Query: 179 HRYVSQRALALLGLPEQESGLVIAHLGNGASICAVRNGRSVDTSMGMTPLEGLMMGTRSG 238
H+YVSQRA +L P + ++ HLGNG+SI AV+NG+S+DTSMG TPLEGL MGTRSG
Sbjct: 183 HKYVSQRAAEILNKPIESLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGLAMGTRSG 242

Query: 239 DVDFGAMAWIAGETRQTLSDLERVANTASGLLGISGLSSDLR-VLEQAWHEGHARARLAI 297
+D ++++ + + ++ + N SG+ GISG+SSD R + + A+ G RA+LA+
Sbjct: 243 SIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRAQLAL 302

Query: 298 KTFVHRIARHIAGHAAALQRLDGIIFTGGIGENSVLIRRLVSERLTVFGLAMDAARNQQP 357
F +R+ + I +AAA+ +D I+FT GIGEN IR + + L G +D +N+
Sbjct: 303 NVFAYRVKKTIGSYAAAMGGVDVIVFTAGIGENGPEIREFILDGLEFLGFKLDKEKNKVR 362

Query: 358 NSAGERLISADGSRVRCAVIPTNEERMIALDAIRL 392
E +IS S+V V+PTNEE MIA D ++
Sbjct: 363 GE--EAIISTADSKVNVMVVPTNEEYMIAKDTEKI 395


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32880SACTRNSFRASE443e-08 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 44.2 bits (104), Expect = 3e-08
Identities = 21/64 (32%), Positives = 26/64 (40%), Gaps = 2/64 (3%)

Query: 73 STWLGRNGIYMEDLYVTPDYRGIGAGKALLKTIAQYAVQRQCGRLEWSVLDWNQPAIDFY 132
S W G +ED+ V DYR G G ALL ++A + L D N A FY
Sbjct: 84 SNWNGY--ALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFY 141

Query: 133 LSIG 136

Sbjct: 142 AKHH 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32860TCRTETA320.001 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.5 bits (74), Expect = 0.001
Identities = 31/134 (23%), Positives = 53/134 (39%), Gaps = 18/134 (13%)

Query: 99 LMRPIGAIVLGAYIDKVGRRKGLIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLL 158
LM+ A VLGA D+ GRR L+V+L+ A ++ P LW ++ IGR++
Sbjct: 54 LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPF-----LW---VLYIGRIV 105

Query: 159 QGFSAGAELGGVSVYLAEIATSGRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAIS 218
G + GA Y+A+I + + + S ++ +G +
Sbjct: 106 AGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGF------- 157

Query: 219 DWGWRIPFLFGCLI 232
PF +
Sbjct: 158 --SPHAPFFAAAAL 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32850TCRTETA351e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 35.2 bits (81), Expect = 1e-04
Identities = 39/157 (24%), Positives = 62/157 (39%), Gaps = 20/157 (12%)

Query: 48 ASDSLLVTLLVAISNFFWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANAPSFL 107
+ ++ L A+ F PV GALSDRFGRR VL L++LA A ++A AP
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVL----LVSLAGAAVDYAIMATAPFLW 97

Query: 108 MMLSVLLWLSFIYGMYNGAMIPALT----EIMPAEVRVAGFSLAYSLATAVFGGFT--PV 161
+ L++ I GA +I + R F ++ G PV
Sbjct: 98 V-----LYIGRIVAGITGATGAVAGAYIADITDGDERARHFGF---MSACFGFGMVAGPV 149

Query: 162 ISTALIEYTGDKASPGYWMSFAAICGLLATCYLYRRS 198
+ + ++ +P + + L C+L S
Sbjct: 150 LGGLMGGFS--PHAPFFAAAALNGLNFLTGCFLLPES 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32820PF05272290.011 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.9 bits (64), Expect = 0.011
Identities = 14/76 (18%), Positives = 32/76 (42%), Gaps = 15/76 (19%)

Query: 18 FIKDENGENRYFHVIKVANPDLIKKDAAVTFEPTTNNKGLSAYAVKVIPESKYIYIAGER 77
++ D G R++ V+ +L+ L + ++ E+ ++Y+AGER
Sbjct: 698 YLFDITGNRRFWPVLVPGRANLV---------------WLQKFRGQLFAEALHLYLAGER 742

Query: 78 LKLTSIKSYVVYREEE 93
+ + +R E+
Sbjct: 743 YFPSPEDEEIYFRPEQ 758


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32800TRNSINTIMINR290.013 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 28.9 bits (64), Expect = 0.013
Identities = 14/56 (25%), Positives = 32/56 (57%), Gaps = 2/56 (3%)

Query: 11 LKAGLVSSKKMAKVQRTAKKSRVQAREAREAVEENKKAQLERDKQLSEQQKQAVLA 66
+ +G + + ++ + AK++ AR+ +AVE N +AQ + Q + +Q++ L+
Sbjct: 308 IPSGELKDDIVEQIAQQAKEAGEVARQ--QAVESNAQAQQRYEDQHARRQEELQLS 361


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32750OMPADOMAIN1025e-26 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 102 bits (256), Expect = 5e-26
Identities = 50/152 (32%), Positives = 69/152 (45%), Gaps = 13/152 (8%)

Query: 407 DWAPPPPPRPVIKQVVQGPQTIRLDSMALFDTGKSALKPGSTKLL--VNSLLGIKAKPGW 464
+ AP P P VQ + L S LF+ K+ LKP L + S L
Sbjct: 195 EAAPVVAPAPAPAPEVQT-KHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDG 253

Query: 465 LIVVAGHTDSIGNDRSNQQLSLKRAEAVRDWMRDTGDVPESCFAVQGYGASRPVASN--- 521
+VV G+TD IG+D NQ LS +RA++V D++ G +P + +G G S PV N
Sbjct: 254 SVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKG-IPADKISARGMGESNPVTGNTCD 312

Query: 522 ------ETPEGRAQNRRVEISLVPQKDACLTP 547
+ A +RRVEI + KD P
Sbjct: 313 NVKQRAALIDCLAPDRRVEIEVKGIKDVVTQP 344


32KPHS_32280KPHS_32150Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_322804152.132604calcium/sodium:proton antiporter
KPHS_322706182.442528cation transport regulator
KPHS_322603174.718408cation transport regulator
KPHS_322504185.064282hypothetical protein
KPHS_322404175.233035putative phosphatase
KPHS_322302174.921386nitrate regulatory protein
KPHS_322200166.350155hypothetical protein
KPHS_322100176.380698nitrate transporter component
KPHS_322001187.128935putative nitrate transporter component, NasE
KPHS_321900166.431539nitrate ABC transporter, inner membrane subunit
KPHS_321800166.100179putative nitrate transport protein nasD
KPHS_321700145.611324putative nitrite reductase
KPHS_32160-2134.234371putative nitrite reductase
KPHS_32150-2123.684541putative nitrate reductase
33KPHS_32040KPHS_31710Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_32040-225-4.601665AsnC family transcriptional regulator
KPHS_32030-128-5.726748**formyltetrahydrofolate deformylase
KPHS_32020031-6.074302hypothetical protein
KPHS_32010-231-5.701098hypothetical protein
KPHS_32000128-5.374056response regulator of RpoS
KPHS_31990232-5.744034UTP--glucose-1-phosphate uridylyltransferase
KPHS_31980129-5.204378global DNA-binding transcriptional dual
KPHS_31970127-5.239637hypothetical protein
KPHS_31960125-5.093678thymidine kinase
KPHS_31950125-4.794472bifunctional acetaldehyde-CoA/alcohol
KPHS_31940013-4.382185hypothetical protein
KPHS_31930-112-3.026790hypothetical protein
KPHS_31920-212-2.678903hypothetical protein
KPHS_31910-111-2.486175oligopeptide ABC transporter substrate-binding
KPHS_31900-114-1.772802oligopeptide transporter permease
KPHS_31890012-1.304911oligopeptide transport permease
KPHS_31880011-1.598021oligopeptide ABC transporter ATP-binding
KPHS_31870113-2.151716oligopeptide ABC transporter ATP-binding
KPHS_31860015-4.682475putative potassium channel protein
KPHS_31850-114-5.046699dsDNA-mimic protein
KPHS_31840-118-4.773361cardiolipin synthetase
KPHS_31830331-5.758022hypothetical protein
KPHS_31820127-4.805779hypothetical protein
KPHS_31810125-4.132702hypothetical protein
KPHS_318000170.317978hypothetical protein
KPHS_31790-1150.321453YciI domain protein
KPHS_31780-1170.351178transporter
KPHS_31770-215-2.267735protein kinase-like protein
KPHS_31760-116-2.980241hypothetical protein
KPHS_31750-118-3.4979386-phosphofructokinase
KPHS_31740121-4.633650hypothetical protein
KPHS_31730121-2.880111hypothetical protein
KPHS_31720220-3.121065threonyl-tRNA synthetase
KPHS_31710320-1.734380hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32020SECA569e-13 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 56.4 bits (136), Expect = 9e-13
Identities = 16/28 (57%), Positives = 20/28 (71%)

Query: 83 IDGTRPLIGRNDPCPCGSGKKFKKCCGQ 110
+GRNDPCPCGSGKK+K+C G+
Sbjct: 872 AQTGERKVGRNDPCPCGSGKKYKQCHGR 899


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32000HTHFIS875e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 5e-21
Identities = 37/152 (24%), Positives = 61/152 (40%), Gaps = 3/152 (1%)

Query: 10 ILIVEDEPVFRSLLHGWLTSLGATTFQAEDGKDALHKMTEVHPDLMICDISMPRMNGLEL 69
IL+ +D+ R++L+ L+ G + + DL++ D+ MP N +L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 70 VETLRNRGEQLPILMISATENMADIAKALRLGVQDVLLKPVKDFDRLRETVYACLYPAMF 129
+ ++ LP+L++SA KA G D L KP D L + L A
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF-DLTELIGIIGRAL--AEP 122

Query: 130 SSRVEEEERLFEDWDALVSNPIAASRLLQELQ 161
R + E +D LV A + + L
Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLA 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31910FLGHOOKAP1300.040 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 29.5 bits (66), Expect = 0.040
Identities = 27/112 (24%), Positives = 44/112 (39%), Gaps = 8/112 (7%)

Query: 392 YNTSDLHKKLAIAAASLWRK------NLGIDVKLVNQEWKTFLDTRHQGTYDVARAGWCA 445
YN + ++ I A + G+ V V +E+ F+ + + +G A
Sbjct: 28 YNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAA-QTQSSGLTA 86

Query: 446 DYNEPTSFLNTMLSDSSMNTAHYKSPAFDKIMAESVKASDEAQRTAAYAKAE 497
Y E S ++ MLS S+ + A F + A D A R A K+E
Sbjct: 87 RY-EQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSE 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31870HTHFIS310.008 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.008
Identities = 9/16 (56%), Positives = 11/16 (68%)

Query: 55 VVGESGCGKSTFARAI 70
+ GESG GK ARA+
Sbjct: 165 ITGESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31780TONBPROTEIN2292e-77 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 229 bits (584), Expect = 2e-77
Identities = 174/241 (72%), Positives = 197/241 (81%), Gaps = 7/241 (2%)

Query: 18 MTLDLPRRFPWPTLLSVAIHGAVVAGLLYTSVHQVIEQPSPTQPIEITMVAPADLEPPPA 77
MTLDLPRRFPWPTLLSV IHGAVVAGLLYTSVHQVIE P+P QPI +TMV PADLEPP A
Sbjct: 1 MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVTPADLEPPQA 60

Query: 78 AQPVVEPVVEPEPEPEPEVAPEPPKEAPVVIHKPEPKPKPKPKPKPKPEKKVEQPKREVK 137
QP EPVVEPEPEPEP PEPPKEAPVVI KP+PKPKPKPKP K + EQPKR+VK
Sbjct: 61 VQPPPEPVVEPEPEPEPI--PEPPKEAPVVIEKPKPKPKPKPKPVKKVQ---EQPKRDVK 115

Query: 138 PAAEPRPASPFENNNTAPARTAPSTSTAAAKPTVTAPSGPRAISRVQPSYPPRAQALRIE 197
P E RPASPFEN A T+ + + A +KP + SGPRA+SR QP YP RAQALRIE
Sbjct: 116 P-VESRPASPFENTAPA-RLTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIE 173

Query: 198 GTVRVKFDVSPDGRIDNLQILSAQPANMFEREVKSAMRRWRYEQGRPGTGVTMTIKFRLN 257
G V+VKFDV+PDGR+DN+QILSA+PANMFEREVK+AMRRWRYE G+PG+G+ + I F++N
Sbjct: 174 GQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKIN 233

Query: 258 G 258
G
Sbjct: 234 G 234


34KPHS_31430KPHS_30840Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_314300163.641023permease protein
KPHS_314200163.695863iron ABC transporter ATP-binding protein
KPHS_31410-1153.802936iron(III) dicitrate transport ATP-binding
KPHS_314000165.422597iron compound ABC superfamily ATP binding
KPHS_313900154.868830iron ABC transporter periplasmic iron-binding
KPHS_313801165.646134LysR family transcriptional regulator
KPHS_313700155.359390NAD dependent epimerase/dehydratase
KPHS_313601164.610979putative TetR family transcriptional regulator
KPHS_313501154.723020HlyD family secretion protein
KPHS_313402162.947522putative efflux protein
KPHS_31330219-0.084750L-lactate dehydrogenase
KPHS_31320341-13.631379hypothetical protein
KPHS_31310245-15.884800hypothetical protein
KPHS_31290127-10.890722hypothetical protein
KPHS_31300123-9.693478hypothetical protein
KPHS_31280017-6.566454hypothetical protein
KPHS_31270-213-3.768162transposase
KPHS_31260-1141.120694iron-sulfur cluster assembly scaffold protein
KPHS_31250-1131.464603cysteine desulfurase activator complex subunit
KPHS_31240-1121.747638cysteine desulfurase
KPHS_312300141.438875cysteine desulfurase activator complex subunit
KPHS_312200170.205015selenocysteine lyase
KPHS_31210120-2.908234cysteine desufuration protein
KPHS_31200223-2.950293putative ATP synthase subunit
KPHS_31190227-4.175624murein lipoprotein
KPHS_31180-114-0.039093pyruvate kinase
KPHS_311700191.945507hypothetical protein
KPHS_31160-1203.078246hypothetical protein
KPHS_311500204.539709hypothetical protein
KPHS_311401205.414306putative regulatory protein TetR
KPHS_311301206.575520putative 4-hydroxyphenylpyruvate dioxygenase
KPHS_311203206.425359major facilitator superfamily permease
KPHS_311102196.747728shikimate 5-dehydrogenase
KPHS_311000176.476921ABC transporter substrate-binding protein
KPHS_310900206.279084putative ABC transporter ATP-binding protein
KPHS_31080-1195.862333ABC transporter permease
KPHS_31070-2204.849949ABC transporter substrate-binding protein
KPHS_31060-1205.321206DNA-binding transcriptional activator MhpR
KPHS_310500195.2780163-(3-hydroxyphenyl)propionate hydroxylase
KPHS_310401195.3729603-(2,3-dihydroxyphenyl)propionate dioxygenase
KPHS_310301206.1216773-(2,3-dihydroxyphenyl)propionate dioxygenase
KPHS_310201195.9377152-hydroxy-6-oxononadienedioate
KPHS_310101187.2173392-keto-4-pentenoate hydratase
KPHS_310001187.030703acetaldehyde dehydrogenase
KPHS_309904197.5969414-hydroxy-2-ketovalerate aldolase
KPHS_309802187.656435putative 3-hydroxyphenylpropionic transporter
KPHS_309701197.377166hypothetical protein
KPHS_30960-1187.386382putative rhodanese-related sulfurtransferase
KPHS_309500176.155279putative cysteine dioxygenase type I
KPHS_309400186.550902putative LysR family regulatory protein
KPHS_309301186.338554putative lavin-dependent oxidoreductase
KPHS_30920-1174.502881putative acyl-CoA dehydrogenase
KPHS_30910-2163.773044putative ABC transporter periplasmic
KPHS_309000164.719456putative ABC-type taurine transport system
KPHS_308901175.625644ABC transporter permease
KPHS_308800185.211680ABC transporter ATPase
KPHS_30870-1175.042017TonB-dependent receptor
KPHS_308601186.505176ferric enterobactin transporter ATP-binding
KPHS_308500195.610746putative iron compound ABC transport system
KPHS_30840-2183.681802iron ABC transporter permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31430TCRTETA667e-14 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 65.6 bits (160), Expect = 7e-14
Identities = 77/373 (20%), Positives = 136/373 (36%), Gaps = 30/373 (8%)

Query: 1 MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLI---GLILGLRTFSQQGMFILGGTLA 57
++L + + +G ++P L LRD ++ S + G++L L Q + G L+
Sbjct: 9 VILSTVALDAVGIGLIMPVLPGLLRD-LVHSNDVTAHYGILLALYALMQFACAPVLGALS 67

Query: 58 DRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGALFSPSIEALLARAGTH 117
DR+G + ++L + ++A LW + +G + G+ GA +
Sbjct: 68 DRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGA-------VAGAYI 120

Query: 118 SQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIALAGAGIFLLALAVLFFC 177
+ RA F + C G V GPV GG++ G A A + L F
Sbjct: 121 ADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFL 180

Query: 178 LPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSY------NQLYLALPV--EIQR 229
LP R PL R+ + ++ + + Q+ AL V R
Sbjct: 181 LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDR 240

Query: 230 SGGREQDLAPLFMLASLLIITLQLPLA-RFARRMGAVRILPVGFLLLSASFASVALFAAA 288
+ +L Q + A R+G R L +G + + +A
Sbjct: 241 FHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFA--- 297

Query: 289 PPAEGWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAHYGALATAGGCAVLA 348
GW+ P + LL G + + PA + ++ +E G G+LA +
Sbjct: 298 --TRGWM-AFPI---MVLLASGGIGM-PALQAMLSRQVDEERQGQLQGSLAALTSLTSIV 350

Query: 349 GNLLLGHLLDLAL 361
G LL + ++
Sbjct: 351 GPLLFTAIYAASI 363


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31410PF05272280.015 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 27.7 bits (61), Expect = 0.015
Identities = 10/20 (50%), Positives = 12/20 (60%)

Query: 33 LLGPNGCGKSSLLRVLAGLR 52
L G G GKS+L+ L GL
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31370NUCEPIMERASE376e-05 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 37.1 bits (86), Expect = 6e-05
Identities = 36/162 (22%), Positives = 60/162 (37%), Gaps = 28/162 (17%)

Query: 6 KVLILGASGGIGGEVARRLVADNWQVRA-----------LKRGAQMRDPEDGIQWIAGDA 54
K L+ GA+G IG V++RL+ QV LK+ + G Q+ D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 LDGGQVAA--AAAGCDVIVH-----AV-----NPPGYRHWRQQVLPMLRNTLQAAERQR- 101
D + A+ + + AV NP Y + L N L+ +
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYAD--SNLTGFL-NILEGCRHNKI 118

Query: 102 ALVVLPGTVYNYGPDA-FPLIAEEAAQQPVTRKGAIRVAMEL 142
++ + YG + P +++ PV+ A + A EL
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31360HTHTETR593e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.9 bits (142), Expect = 3e-13
Identities = 31/183 (16%), Positives = 52/183 (28%), Gaps = 10/183 (5%)

Query: 4 FSRYGYEKTTVTDLAKAIGFSKAYIYKFFDSKQAIGEAICASRLEKIMVAVSEAIADAPS 63
FS+ G T++ ++AKA G ++ IY F K + I I E A P
Sbjct: 24 FSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPG 83

Query: 64 ASEK-----LRRLFR-ALTEAGSELFFE--DRKLYDIAAVAARDKWPSTEQYAGHLQQLI 115
L + +TE L E K + +A + I
Sbjct: 84 DPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA--QRNLCLESYDRI 141

Query: 116 GQILVEGRQAGEFERKTPLDEATLAVYMVMCPFINPVQLQYNLDTAPTAAVLLASLILRS 175
Q L +A A + + + + A +++L
Sbjct: 142 EQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEM 201

Query: 176 LSP 178

Sbjct: 202 YLL 204


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31350RTXTOXIND423e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 3e-06
Identities = 19/92 (20%), Positives = 36/92 (39%), Gaps = 9/92 (9%)

Query: 70 GKVLERRVETGQSVKRGQLLLRLDPADLALQAQSQQRAVDAARARAKKAANDLARYRGLV 129
V E V+ G+SV++G +LL+L Q ++ AR L + R +
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQAR---------LEQTRYQI 155

Query: 130 ASGAISAAEFDQINAAAEAARADLSAAQAQAN 161
S +I + ++ E ++S +
Sbjct: 156 LSRSIELNKLPELKLPDEPYFQNVSEEEVLRL 187



Score = 31.3 bits (71), Expect = 0.005
Identities = 18/84 (21%), Positives = 32/84 (38%), Gaps = 4/84 (4%)

Query: 178 GVVVETLAEPGQVVSAGQVVIRLARAGQREARVQLPETLRPAVGSEALATRYGSESQPV- 236
+V E + + G+ V G V+++L G ++ +L A TRY S+ +
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQA---RLEQTRYQILSRSIE 161

Query: 237 TATLRLLSDAADATTRTFEARYVL 260
L L + + VL
Sbjct: 162 LNKLPELKLPDEPYFQNVSEEEVL 185



Score = 30.2 bits (68), Expect = 0.015
Identities = 12/128 (9%), Positives = 37/128 (28%), Gaps = 15/128 (11%)

Query: 103 SQQRAVDAARARAKKAANDLARYRGLVAS--GAISAAEFDQINAAAEA----------AR 150
++ ++ + A N+L Y+ + I +A+ +
Sbjct: 250 AKHAVLEQENKYVE-AVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTT 308

Query: 151 ADLSAAQAQANVAQNATGYAGLLADADGVVVE-TLAEPGQVVSAGQVVIRLARAGQR-EA 208
++ + + + + A V + + G VV+ + ++ + E
Sbjct: 309 DNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEV 368

Query: 209 RVQLPETL 216
+
Sbjct: 369 TALVQNKD 376


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31340ACRIFLAVINRP438e-139 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 438 bits (1128), Expect = e-139
Identities = 233/1055 (22%), Positives = 422/1055 (40%), Gaps = 71/1055 (6%)

Query: 8 LSALAVRERSVTLFLIILISVAGLVAFFGLGRAEDPPFTVKQMTVITVWPGATAQEMQDQ 67
++ +R L I++ +AG +A L A+ P ++V +PGA AQ +QD
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 68 VAEPLEKRLQELKWYDRTETYT-RPGMALITLSLQDQTPP----SEVPEQFYQARKKLGD 122
V + +E+ + + + + G ITL+ Q T P +V + A L
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLL-- 118

Query: 123 EAKNLPAGVSGPMMNDEFADVTFALFAL--KARGEPPRQLVRD--AEALRQQLLHVPGVK 178
P V ++ E + ++ + A + + D A ++ L + GV
Sbjct: 119 -----PQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVG 173

Query: 179 KVNILGEQ-AERIYLSFSHDRLATLGLSPEAIFAALNSQNVLTAAGAI---ETRGGQIF- 233
V + G Q A RI+L D L L+P + L QN AAG + GQ
Sbjct: 174 DVQLFGAQYAMRIWLDA--DLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLN 231

Query: 234 --IRLDGAFDRLQQIRDTPIIAG--GRTLKLADVATVERGYEDPATFLIRNQGEPALLLG 289
I F ++ + G ++L DVA VE G E+ R G+PA LG
Sbjct: 232 ASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIA-RINGKPAAGLG 290

Query: 290 VVMREGWNGLALGKALDAETTSINQSLPLGMSLTKVTDQSVNISAAVDEFMIKFFVALLV 349
+ + G N L KA+ A+ + P GM + D + + ++ E + F A+++
Sbjct: 291 IKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIML 350

Query: 350 VMSVCFVSMG-WRVGVVVAAAVPLTLAVVFVVMEATGKNFDRITLGSLILALGLLVDDAI 408
V V ++ + R ++ AVP+ L F ++ A G + + +T+ ++LA+GLLVDDAI
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAI 410

Query: 409 IAIEMMV-VKMEEGYDRLKASAYAWSHTAAPMLAGTLVTAVGFMPNGFAQSTAGEYASNV 467
+ +E + V ME+ +A+ + S ++ +V + F+P F + G
Sbjct: 411 VVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQF 470

Query: 468 FWIVGIALIASWIVAVIFTPWLGVHLLPNRKPAAAGHAALYDT----------PRYQHFR 517
+ A+ S +VA+I TP L LL KP +A H H+
Sbjct: 471 SITIVSAMALSVLVALILTPALCATLL---KPVSAEHHENKGGFFGWFNTTFDHSVNHYT 527

Query: 518 RLLTRVIAHKWRVAAGVVALFIVAILGMSVVKKQFFPTSDRPEVLVEVQLPYGSSISQTS 577
+ +++ R + ++ + F P D+ L +QLP G++ +T
Sbjct: 528 NSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQ 587

Query: 578 AAAAKIEHWLHLQPEVKIVTSYIGQGAPRFYLAMAPELPDP--SFAKLMVLTDGQGARE- 634
++ + L+ E V S + + + + +F L + G
Sbjct: 588 KVLDQVTDYY-LKNEKANVESVFTVNG----FSFSGQAQNAGMAFVSLKPWEERNGDENS 642

Query: 635 --ALKRRLREAV-----ANGLAPEARVRVTQLVFGPYSPYPVAWRVMGPDPHALLDIAER 687
A+ R + + + V + + +G D AL +
Sbjct: 643 AEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHD--ALTQARNQ 700

Query: 688 VKSVLQASPL-MRTVNTDWGSRVPVMHFSLNQDRLQASGLSSQSVAQQLQFLLSGIPITT 746
+ + P + +V + ++Q++ QA G+S + Q + L G +
Sbjct: 701 LLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVND 760

Query: 747 VREDIRAVQVIGRAAGDIRLDPAKIADFTLVGSGGQRVPLSQIGDVSIRMEDPLLRRRDR 806
+ R ++ +A R+ P + + + G+ VP S P L R +
Sbjct: 761 FIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNG 820

Query: 807 TPTITVRGDVAENLQPPDVSTALMKPLQPIIDSLPPGYRIETAGSIEESGKATRAMVPLF 866
P++ ++G+ A P S M ++ + LP G + G + + L
Sbjct: 821 LPSMEIQGEAA----PGTSSGDAMALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALV 876

Query: 867 PIMIALTLLIIILQVRSLSAMVMVFLTAPLGLIGVVPTLLLFNQPFGINALVGLIALSGI 926
I + L + S S V V L PLG++GV+ LFNQ + +VGL+ G+
Sbjct: 877 AISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGL 936

Query: 927 LMRNTLILIGQIHHNQQA-GLDPFHAVVEATVQRARPVLLTALAAILAFIPLTHSVFWGT 985
+N ++++ + G A + A R RP+L+T+LA IL +PL S G+
Sbjct: 937 SAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGS 996

Query: 986 -----LAYTLIGGTLGGTIMTLIFLPAMYAIWFRI 1015
+ ++GG + T++ + F+P + + R
Sbjct: 997 GAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031



Score = 78.7 bits (194), Expect = 8e-17
Identities = 87/510 (17%), Positives = 181/510 (35%), Gaps = 36/510 (7%)

Query: 529 RVAAGVVALFIVA--ILGMSVVKKQFFPTSDRPEVLVEVQLPYGSSISQTSAAAAKIEHW 586
+ A V+A+ ++ L + + +PT P V V P + + IE
Sbjct: 9 PIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQN 68

Query: 587 LHLQPEVKIVTSY-IGQGAPRFYLAMAPELPDPSFAKLMVLTDGQGAREALKRRLREAVA 645
++ + ++S G+ L DP A++ V Q + L + V
Sbjct: 69 MNGIDNLMYMSSTSDSAGSVTITLTFQSGT-DPDIAQVQV----QNKLQLATPLLPQEVQ 123

Query: 646 NGLAPEARVRVTQLVFGPYSPYPVAWRVMGPDPHALLDIAER-VKSVLQASPLMRTVNTD 704
V + + + + D + D VK L + V
Sbjct: 124 Q---QGISVEKSSSSYLMVAGFVSDNPGTTQD--DISDYVASNVKDTLSRLNGVGDVQL- 177

Query: 705 WGSRVPVMHFSLNQDRLQASGLSSQSVAQQLQF----LLSGIPITTVREDIRAVQVIGRA 760
+G++ M L+ D L L+ V QL+ + +G T + + A
Sbjct: 178 FGAQY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 761 AGDIRLDPAKIADFTLVGSG-GQRVPLSQIGDVSIRMED-PLLRRRDRTPTITVRGDVAE 818
+ +P + TL + G V L + V + E+ ++ R + P + +A
Sbjct: 237 QTRFK-NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLAT 295

Query: 819 NLQPPDVSTALMKPLQPIIDSLPPGYRIE----TAGSIEESGKATRAMVPLFPIMIALTL 874
D + A+ L + P G ++ T ++ S +V I L
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLS---IHEVVKTLFEAIMLVF 352

Query: 875 LIIILQVRSLSAMVMVFLTAPLGLIGVVPTLLLFNQPFGINALVGLIALSGILMRNTLIL 934
L++ L ++++ A ++ + P+ L+G L F + G++ G+L+ + +++
Sbjct: 353 LVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVV 412

Query: 935 IGQIH-HNQQAGLDPFHAVVEATVQRARPVLLTALAAILAFIPL-----THSVFWGTLAY 988
+ + + L P A ++ Q ++ A+ FIP+ + + +
Sbjct: 413 VENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSI 472

Query: 989 TLIGGTLGGTIMTLIFLPAMYAIWFRIRPE 1018
T++ ++ LI PA+ A +
Sbjct: 473 TIVSAMALSVLVALILTPALCATLLKPVSA 502


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31320HTHTETR647e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 64.3 bits (156), Expect = 7e-15
Identities = 34/172 (19%), Positives = 62/172 (36%), Gaps = 6/172 (3%)

Query: 7 QKGRPKDPLKTQAILQAARKLFLEQGLE-VTTAEIARVAGVAKATLYANFSDKEHLIEAV 65
+K + + Q IL A +LF +QG+ + EIA+ AGV + +Y +F DK L +
Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62

Query: 66 LRQESD--LTISDHDFAQRHHLPLIEVLTAFGYRFVRFINQRELTGWDRLIASAAVRHPD 123
+ A+ PL + + + + +I +
Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122

Query: 124 LPG--RFYAAGPGRAQQMLEAIIAEAIEAGTLRA-CDPQEAADELAGLWLGM 172
+ + + +E + IEA L A + AA + G G+
Sbjct: 123 MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31190VACJLIPOPROT270.005 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 27.2 bits (60), Expect = 0.005
Identities = 17/45 (37%), Positives = 26/45 (57%), Gaps = 1/45 (2%)

Query: 5 KLVLGAVILGSTLLAGCSSNAKIDQLSSD-VQTLNAKVDQLSNDV 48
KL L A+ LG+TLL GC+S+ Q SD ++ N + + +V
Sbjct: 2 KLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNV 46


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31140HTHTETR662e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 65.8 bits (160), Expect = 2e-15
Identities = 29/162 (17%), Positives = 61/162 (37%), Gaps = 4/162 (2%)

Query: 6 HDEAQSLKARIFSAAIAVFAEHGLSGARMEQIATEAQTTKRMVVYYFKSKEQLYQEVLQH 65
EAQ + I A+ +F++ G+S + +IA A T+ + ++FK K L+ E+ +
Sbjct: 6 KQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWEL 65

Query: 66 VYARIRETEQQLGLENVPPVEALVR---LVRWSVRYHATHADYMRVICMENMQR-GKWLK 121
+ I E E + + +++R + + I + G+
Sbjct: 66 SESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAV 125

Query: 122 SSGELKPLNRTALSILEDILLRGQQQGVFQAGLDARDVHRLI 163
+ L + +E L + + A L R ++
Sbjct: 126 VQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31120TCRTETA486e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 47.5 bits (113), Expect = 6e-08
Identities = 69/370 (18%), Positives = 124/370 (33%), Gaps = 43/370 (11%)

Query: 64 GILFSAFAWTYALAQIPGGLFLDRFGNKVTYFLSLTLWSLFTLFHGMAVGLKTLLLCRFG 123
GIL + +A G DRFG + +SL ++ A L L + R
Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105

Query: 124 LGISEAPCFPVNSRVVSAWFPQQERAKA----TAVYTVGEYLGLACFAPLLFWIMDGFGW 179
GI+ A V ++ ERA+ +A + G G P+L +M GF
Sbjct: 106 AGITGAT-GAVAGAYIADITDGDERARHFGFMSACFGFGMVAG-----PVLGGLMGGFSP 159

Query: 180 RVLFVSVGAVGILFALVWWRCYREPHEDPRLSQQEREHIENGGGLSAPTDQQVAFSWPLV 239
F + A+ L L E H+ R + + +F W
Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREA-----------LNPLASFRWARG 208

Query: 240 RQLLSKRQIIGASIGQFAGNTVLVFFLTWFPTWLATERHMPWLKVGFFSILPFVAAAGGV 299
+++ + + ++ + E W + + AA G+
Sbjct: 209 MTVVAALMAVFFIMQLVGQVPAALWVIF-------GEDRFHWDA----TTIGISLAAFGI 257

Query: 300 M---FGGWLSDKLLKATGSANLGRKLPIVAGLL--MASCIITANWLESDLAVILVMSFAF 354
+ ++ + LG + ++ G++ I+ A +A +++ A
Sbjct: 258 LHSLAQAMITGPVAA-----RLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLAS 312

Query: 355 FGQGMVGLGWTLISDIAPKGLGGLTGGLFNFCANLAGILTPLVIGFIVAGFGNFFYALIY 414
G GM L ++S + G G +L I+ PL+ I A + +
Sbjct: 313 GGIGMPALQ-AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAW 371

Query: 415 IGGAALLGVV 424
I GAAL +
Sbjct: 372 IAGAALYLLC 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31010PF06438280.042 Heme acquisition protein HasAp
		>PF06438#Heme acquisition protein HasAp

Length = 205

Score = 27.6 bits (61), Expect = 0.042
Identities = 25/121 (20%), Positives = 39/121 (32%), Gaps = 17/121 (14%)

Query: 139 VGSRIRDWSIGFVD-------TVADNASCGLYVIGGPAQRPAGLDLKQCAMHMTRNQE-L 190
V + DWS F D V + + G GP D Q A+ T +
Sbjct: 16 VADYLADWSAYFGDVNHRPGQVVDGSNTGGFN--PGP------FDGSQYALKSTASDAAF 67

Query: 191 VSSGRGSECLGHPLNAAVWLARKLASLGEPLRAGDIVLTGALG-PMVTINEGDSFVAHIE 249
++ G L + +W +LG+ L G AL V+ + +
Sbjct: 68 IAGGDLHYTLFSNPSHTLWGKLDSIALGDTLTGGASSGGYALDSQEVSFSNLGLDSPIAQ 127

Query: 250 G 250
G
Sbjct: 128 G 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30980TCRTETB484e-08 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 48.0 bits (114), Expect = 4e-08
Identities = 81/404 (20%), Positives = 151/404 (37%), Gaps = 60/404 (14%)

Query: 14 VTIGLCFMVALMEGLDLQAAGIAAVGMAQAFALDKMQMGWIFSAGILGLLPGALVGGMLA 73
+ I LC + L+ ++ +A F W+ +A +L G V G L+
Sbjct: 15 ILIWLCILS-FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLS 73

Query: 74 DRHGRKRILLGSVLLFGLFSLATALAWS-FPTLLLARLLTGVGLGAALPNLIA-LTSEAA 131
D+ G KR+LL +++ S+ + S F L++AR + G G AA P L+ + +
Sbjct: 74 DQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAG-AAAFPALVMVVVARYI 132

Query: 132 GSRFRGRAVSLMYCGVPIGAALAAALGFSGFAAAWQIIFW----IGGVVPLLLIPLLMRW 187
RG+A L+ V +G + A+G I W + ++ ++ +P LM+
Sbjct: 133 PKENRGKAFGLIGSIVAMGEGVGPAIG----GMIAHYIHWSYLLLIPMITIITVPFLMKL 188

Query: 188 LPESQAFQRA---------EASVPLRTLFAPGQAAATLLLWLGYFFTLLVVYMLINWLPM 238
L + + + LF + + L++ + F + V ++ P
Sbjct: 189 LKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFL-IFVKHIRKVTDPF 247

Query: 239 LLVGQGFRASQAAGVMFSLQI-GAACGTLLLGALMDK--------------LTPLRMSLL 283
+ G G GV+ I G G + + M K + P MS++
Sbjct: 248 VDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVI 307

Query: 284 IYS---GILAS------LLALGSASSLTGMLLAGFV----------AGLFATGGQSVLYA 324
I+ GIL +L +G L A F+ +F GG S
Sbjct: 308 IFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKT 367

Query: 325 LAPLFYPAAIRATGVGTAVA----VGRLGAMSGPLLAGKMLALG 364
+ ++++ G ++ L +G + G +L++
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIP 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30880PF05272290.020 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.020
Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 4/32 (12%)

Query: 38 LRPG---ESVALL-GPSGCGKSTLLRLLAGLE 65
+ PG + +L G G GKSTL+ L GL+
Sbjct: 589 MEPGCKFDYSVVLEGTGGIGKSTLINTLVGLD 620


35KPHS_30740KPHS_29880Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_30740-1185.239352glyoxalase I, nickel isomerase
KPHS_30730-1195.314794putative transporter protein
KPHS_307200183.318274putative metal-dependent
KPHS_30710-1182.475369putative metal-dependent
KPHS_30700-2162.831003formate dehydrogenase H, selenopolypeptide
KPHS_30690-1142.463083hypothetical protein
KPHS_306800141.177946hypothetical protein
KPHS_306700161.808130putative ABC transporter periplasmic binding
KPHS_30660-2193.354118ABC transporter permease
KPHS_306500194.217702putative ABC transport system ATP-binding
KPHS_306400204.747085glyoxalase
KPHS_306300225.120191gluconate 2-dehydrogenase
KPHS_306200235.728208gluconate 2-dehydrogenase
KPHS_306100206.923181gluconate dehydrogenase cytochrome c subunit
KPHS_306003227.326074cytochrome c biogenesis protein CcmA
KPHS_305904236.640954heme exporter permease component
KPHS_305803215.637850heme ABC transporter membrane protein
KPHS_305702205.698933heme exporter protein C
KPHS_305603205.945738cytochrome c biogenesis
KPHS_305503154.409245cytochrome c-type biogenesis protein
KPHS_30540126-1.878089heme lyase/disulfide oxidoreductase
KPHS_30530240-7.950137cytochrome c-type biogenesis protein CcmH
KPHS_30520148-10.984074hypothetical protein
KPHS_30510253-13.308812cytochrome c-type biogenesis protein CcmI
KPHS_30500560-16.363788hypothetical protein
KPHS_30490560-16.484074peptidase U61 LD-carboxypeptidase A
KPHS_30480353-13.882482glycosyltransferase
KPHS_30470352-12.928069sensor histidine protein kinase (RstA
KPHS_30460244-10.457033response regulator
KPHS_30450129-6.567917putative transport protein
KPHS_30440-123-4.517802hypothetical protein
KPHS_30430-118-3.142091acridine efflux pump
KPHS_30420-2171.935909putative acetyl transferase
KPHS_30410-1224.301412short chain dehydrogenase
KPHS_304001225.417869acetolactate synthase large subunit
KPHS_303901245.934344acetolactate decarboxylase
KPHS_303802257.114604LysR family transcriptional regulator
KPHS_303701257.5027493-hydroxybutyrate dehydrogenase
KPHS_30360-1247.330409citrate transporter
KPHS_303500227.2255293-hydroxybutyryl-CoA dehydrogenase
KPHS_303400216.061083beta-ketothiolase
KPHS_303300162.397521acetyl-CoA acetyltransferase
KPHS_303200160.939376putative acetyl-CoA:acetoacetyl-CoA transferase
KPHS_30310-1150.4025633-oxoadipate CoA-transferase subunit A
KPHS_30300-2151.273199LysR family transcriptional regulator
KPHS_30290-3140.767492hypothetical protein
KPHS_30280-2141.622351hypothetical protein
KPHS_302600173.670693hypothetical protein
KPHS_30270-1173.794441chromate ion transporter (CHR) family chromate
KPHS_30250-1183.786009FAD-binding protein
KPHS_302400204.887743ABC transporter ATP-binding protein
KPHS_302300174.829050ABC transporter ATP-binding protein
KPHS_302201173.941817ABC transporter substrate-binding protein
KPHS_302102164.200590putative creatininase
KPHS_302002154.971570putative endoribonuclease
KPHS_301902164.645191putative cytosine deaminase
KPHS_301802152.939339ferredoxin
KPHS_301703162.598687LysR family transcriptional regulator
KPHS_301600141.011526ABC transport system periplasmic binding
KPHS_301500160.360673amidohydrolase
KPHS_30140121-1.8901942OG-Fe(II) oxygenase superfamily dioxygenase
KPHS_30120225-1.486614hypothetical protein
KPHS_30130224-1.187997LysR family transcriptional regulator
KPHS_30110121-0.887052putative NADPH:quinone reductase and related
KPHS_301000170.860136LysR family transcriptional regulator
KPHS_300900141.826391major facilitator superfamily protein
KPHS_300801142.923805putative transport protein
KPHS_300702153.254582hypothetical protein
KPHS_300602153.300833hypothetical protein
KPHS_30050-1152.816934hypothetical protein
KPHS_300400163.139036oligopeptide ABC transporter ATP-binding
KPHS_300300172.878102putative ABC transport system ATP-binding
KPHS_30020-1151.634496oligopeptide ABC transport system permease
KPHS_30010-1151.312254oligopeptide ABC transport system permease
KPHS_30000-1140.672272oligopeptide ABC transporter
KPHS_299900160.846357putative chloramphenicol acetyltransferase
KPHS_29980-114-0.136465glyoxalase I, nickel isomerase
KPHS_29970-114-1.814279putative DeoR-type transcriptional regulator
KPHS_29960217-3.889467amino acid exporter
KPHS_29950222-6.013206putative carbonic anhydrase
KPHS_29940-126-3.172032hypothetical protein
KPHS_29930-123-1.914174hypothetical protein
KPHS_299200200.378102hypothetical protein
KPHS_299101221.557467hypothetical protein
KPHS_299001232.129942hypothetical protein
KPHS_298902202.233657beta-lactamase
KPHS_298803240.870043hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30730TCRTETA310.006 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 31.3 bits (71), Expect = 0.006
Identities = 63/343 (18%), Positives = 116/343 (33%), Gaps = 23/343 (6%)

Query: 48 GLLAALPPAGMMISSFLSPALCRRVEMGVLLSGSLILLALATIASCMTTDMTLLLLPRLL 107
G+L AL + + AL R +L SL A+ + +L + R++
Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105

Query: 108 TGLASGVIIVLGESWITGGAAGSQRATLTGLYASAFTGCQLAGPLL------ISVGPAWQ 161
G+ V G ++I G +RA G ++ F +AGP+L S +
Sbjct: 106 AGITGATGAVAG-AYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFF 164

Query: 162 TSALIAIVAVTAVCLLMLRHLPTGTRE------SLGERASWRSLGAFLPVLASGVFCFAF 215
+A + + C L+ R + W + L + F
Sbjct: 165 AAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQL 224

Query: 216 FDASILALLPLYGMDK-GLNEGLAVLLVTVVLTGDAMFQTPL-GWLADRVGIRRVHLSCA 273
AL ++G D+ + + + ++ Q + G +A R+G RR +
Sbjct: 225 VGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGM 284

Query: 274 VVFSLSLLALPLMLGSRIQLMAICLLLGAAAG--ALYTLSLVRAGKTFNGQKLIMINALF 331
+ + L + + LL G AL + + + GQ +
Sbjct: 285 IADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQ----LQGSL 340

Query: 332 GFFWSAGSVAGPVVSGMLIG--ITGYDGLIVTLVASGVLFLLI 372
S S+ GP++ + IT ++G A+ L L
Sbjct: 341 AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLP 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30650PF05272280.044 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.044
Identities = 12/34 (35%), Positives = 17/34 (50%)

Query: 31 VVSLLGPSGSGKTTLLRAVAGLEKPTSGRIAIGN 64
V L G G GK+TL+ + GL+ + IG
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGT 631


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30460HTHFIS787e-19 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 77.9 bits (192), Expect = 7e-19
Identities = 29/118 (24%), Positives = 59/118 (50%), Gaps = 1/118 (0%)

Query: 7 IIVAEDDDDIAAILTGYLRKAGMKTLRAEDGEQAINLTRLNKPDLLLLDIHLPVYDGWNV 66
I+VA+DD I +L L +AG + DL++ D+ +P + +++
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 67 LTTLRKE-TNVPVIMVTALDQDVDKLMGLRLGADDYVIKPFNPSEVIARVEAVLRRTR 123
L ++K ++PV++++A + + + GA DY+ KPF+ +E+I + L +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30430ACRIFLAVINRP10600.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1060 bits (2744), Expect = 0.0
Identities = 502/1031 (48%), Positives = 690/1031 (66%), Gaps = 7/1031 (0%)

Query: 1 MPHFFIERPIFAWVIALFIVLTGLLSIPRLPVAQYPEVAPPGIIISVSYPGASPEVMNTS 60
M +FFI RPIFAWV+A+ +++ G L+I +LPVAQYP +APP + +S +YPGA + + +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VVSLIEREISSVDNLLYFESSSDTTGMASITVTFKPGTDIKLAQMDLQNQIKIVESRLPQ 120
V +IE+ ++ +DNL+Y S+SD+ G +IT+TF+ GTD +AQ+ +QN++++ LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 SVRQNGINVEAANSGFLMMVGLKSPSGAYQEADLSDYFARNVTDELRRVPGVGKVQLFGG 180
V+Q GI+VE ++S +LM+ G S + + D+SDY A NV D L R+ GVG VQLFG
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 EKALRIWLDPMKLHSYGLSVTDVLSAISQQNVIVSPGRTGDEPATSSQEVTYPITVKGQL 240
+ A+RIWLD L+ Y L+ DV++ + QN ++ G+ G PA Q++ I + +
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 SSVEEFRNITIKSQVSAARVTLADVARVESGLQSYAFGIRENGVPATAAAIQLSPGANAI 300
+ EEF +T++ + V L DVARVE G ++Y R NG PA I+L+ GANA+
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 STASGIRARLTELSGVLPEGMTFTVPFDTAPFVKLSILKVVETFVEAMVLVFFVMLLFLH 360
TA I+A+L EL P+GM P+DT PFV+LSI +VV+T EA++LVF VM LFL
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 KIRCTLIPAIVAPVALLGTFTVMLLSGYSINILTMFGMILAIGIIVDDAIVVVENVERLM 420
+R TLIP I PV LLGTF ++ GYSIN LTMFGM+LAIG++VDDAIVVVENVER+M
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 EDKKMSPQDATREAMREITPAIIGITLVLTAVFIPMAFASGSVGIIYRQFSISMAISILL 480
+ K+ P++AT ++M +I A++GI +VL+AVFIPMAF GS G IYRQFSI++ ++ L
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SAFLALTLTPALCATLLKP-HGIHQGKSSVFSAWFNAHFHRLTSFYATGLGFVLKRTGRM 539
S +AL LTPALCATLLKP H F WFN F + Y +G +L TGR
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 540 MMIYAALCLALFAGLSTLPSSFLPDEDQGYFMSSIQLPSDATMQRTLKVVDTFEEEI--A 597
++IYA + + LPSSFLP+EDQG F++ IQLP+ AT +RT KV+D +
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 598 HRQAVESNIMILGFGFSGSGQNSAMAFTTLKDWRQRKGT--TAQEEADHIRSQMANVPDA 655
+ VES + GF FSG QN+ MAF +LK W +R G +A+ + ++ + D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 656 VTMSLLPPAISDMGTSSGFTYYLQDRGGKGYQALKKAADELIVQANHNP-HLADVYIDGL 714
+ PAI ++GT++GF + L D+ G G+ AL +A ++L+ A +P L V +GL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 715 GEGTSLSLHVDREKAEAMGVSFDEINQTISVAAGSNYVNDYTNNGRVQQVIVQADAPYRM 774
+ L VD+EKA+A+GVS +INQTIS A G YVND+ + GRV+++ VQADA +RM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 775 QPEQLLALSVKNRLGQMLPLSTFVTLSWNVAPQQLIRYQGYPAIRITGSAAQGKSSGTAM 834
PE + L V++ G+M+P S F T W +L RY G P++ I G AA G SSG AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 835 AAMDNLAKHLPPGFAGEWAGSSLQEKESASQLPGLIVLSVLVVFMVLAALYESWSVPFAV 894
A M+NLA LP G +W G S QE+ S +Q P L+ +S +VVF+ LAALYESWS+P +V
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 895 MLVVPLGLLGAVLAVSVTNMTNDVFFKVGLITLIGLSAKNAILIIEFARQLM-KEGKSLI 953
MLVVPLG++G +LA ++ N NDV+F VGL+T IGLSAKNAILI+EFA+ LM KEGK ++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 954 DATLTAAKLRLRPILMTSLAFTLGVVPLMLASGASDSTQHAIGTGVFGGMISGTLLAIFF 1013
+ATL A ++RLRPILMTSLAF LGV+PL +++GA Q+A+G GV GGM+S TLLAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1014 VPVFFVTITRF 1024
VPVFFV I R
Sbjct: 1021 VPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30410DHBDHDRGNASE1241e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (312), Expect = 1e-36
Identities = 74/254 (29%), Positives = 114/254 (44%), Gaps = 10/254 (3%)

Query: 3 KVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATAKAVASEINQAGGRAMAVKVDVSD 62
K+A +TGA QGIG+A+A L G +A DYN + V S + A A DV D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 63 RDQVFAAVEQARKTLGGFDVIVNNAGVAPSTPIESITPEIVDKVYNINVKGVIWGIQAAV 122
+ + + +G D++VN AGV I S++ E + +++N GV ++
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128

Query: 123 EAFKKEGHGGKIINACSQAGHVGNPELAVYSSSKFAVRGLTQTAARDLAPLGITVNGYCP 182
+ G I+ S V +A Y+SSK A T+ +LA I N P
Sbjct: 129 KYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 183 GIVKTPM----WAEIDRQVSEAAGKPLGYGTAEFAKRITLGRLSEPEDVAACVSYLASPD 238
G +T M WA+ + G F I L +L++P D+A V +L S
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGS-----LETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 239 SDYMTGQSLLIDGG 252
+ ++T +L +DGG
Sbjct: 243 AGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30370DHBDHDRGNASE1154e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 115 bits (288), Expect = 4e-33
Identities = 66/255 (25%), Positives = 105/255 (41%), Gaps = 9/255 (3%)

Query: 3 LHGKTALVTGSTSGIGLGIAKVLAQAGAQLVLNGFGDSSHARAE--VAALGKIPGYHDAD 60
+ GK A +TG+ GIG +A+ LA GA + + + + A + AD
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 61 LRDVGQIEAMMRYAESTFGGVDIVINNAGIQHVAPVEQFPVDKWNDILAINLSSVFHTTR 120
+RD I+ + E G +DI++N AG+ + ++W ++N + VF+ +R
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 121 LALPGMRQRNWGRIINIASVHGLVASKEKSAYVAAKHAVVGLTKTVALETARSGITCNAI 180
M R G I+ + S V +AY ++K A V TK + LE A I CN +
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 181 CPGWVLTPLVQQQIDKRIAEGVDPEQASAQLLAEKQ---PSGEFVTPQQLGEMALFLCSD 237
PG T + EQ L + P + P + + LFL S
Sbjct: 186 SPGSTETDMQWSLWADENGA----EQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 238 AAAQVRGAAWNMDGG 252
A + +DGG
Sbjct: 242 QAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30360ABC2TRNSPORT300.028 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 29.5 bits (66), Expect = 0.028
Identities = 32/129 (24%), Positives = 47/129 (36%), Gaps = 15/129 (11%)

Query: 7 ALAALALLMLAAYRGY----SVILFAPIAALGAVLLTDPGAVGPA----------FTGLF 52
ALA + ++AA GY S++ P+ AL + G V A + L
Sbjct: 126 ALAGAGIGVVAAALGYTQWLSLLYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLV 185

Query: 53 MEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAIRILGRRHAIPVIVLVCALLTY 112
+ ++ FPV L VF S SI +LG + V V AL Y
Sbjct: 186 ITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIMLGHP-VVDVCQHVGALCIY 244

Query: 113 GGVSLFVVA 121
+ F+
Sbjct: 245 IVIPFFLST 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30230PF05272290.024 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.9 bits (64), Expect = 0.024
Identities = 15/42 (35%), Positives = 21/42 (50%), Gaps = 1/42 (2%)

Query: 53 ITLLGPSGCGKSTLLKMVAGLVEPSDGKLMLW-RRDSREKAQ 93
+ L G G GKSTL+ + GL SD + +DS E+
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIA 640


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30150FERRIBNDNGPP320.005 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 31.8 bits (72), Expect = 0.005
Identities = 16/72 (22%), Positives = 32/72 (44%), Gaps = 9/72 (12%)

Query: 188 LVSRYHDPRPDSLRRVVMAPTTVLHSAPGAQ-LREMAKLARQLGIRL------HSHLSET 240
+ S + P P+ L R+ AP + + G Q L K ++ L +HL++
Sbjct: 101 VWSAGYGPSPEMLARI--APGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQY 158

Query: 241 VDYLDAARQKFA 252
D++ + + +F
Sbjct: 159 EDFIRSMKPRFV 170


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30090TCRTETB673e-15 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 66.8 bits (163), Expect = 3e-15
Identities = 41/163 (25%), Positives = 72/163 (44%), Gaps = 3/163 (1%)

Query: 39 PAIQQSLGGSPAALSWLTNGFMLTFGSFLLAAGVTADAIDRKRIFIAGAALLCLSSLLFC 98
P I PA+ +W+ FMLTF G +D + KR+ + G + C S++
Sbjct: 38 PDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGF 97

Query: 99 LTHNLFLSGVL-RALQGLAAAMILASGSAALAQLYDGAQRTRAFSILGTVFGIGLAFGPL 157
+ H+ F ++ R +QG AA A +A+ R +AF ++G++ +G GP
Sbjct: 98 VGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPA 157

Query: 158 LIGFMIDAVGWRGVYALFALLSAGVLLIGLVSLPATEKASRGH 200
+ G + + W + + + V L+ L E +GH
Sbjct: 158 IGGMIAHYIHWSYLLLIPMITIITVPF--LMKLLKKEVRIKGH 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30080TCRTETB452e-07 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 44.9 bits (106), Expect = 2e-07
Identities = 36/195 (18%), Positives = 76/195 (38%), Gaps = 10/195 (5%)

Query: 14 GFLSLTTLALLIASGGLFVAFVVRCRRVNNPVLELSLLRHPRFVGVLLLPVATCCCYVVL 73
FL ++ L+ LI FV R+V +P ++ L ++ F+ +L
Sbjct: 224 SFLIVSVLSFLI--------FVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGF 275

Query: 74 LIIVPLHFMGGEGMSESQ-SALYLMALTTPMLVFPSVAALLTRWFSPGQVSTAGLMMASV 132
+ +VP +S ++ ++ + T +++F + +L P V G+ SV
Sbjct: 276 VSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV 335

Query: 133 GLLLLGDAFHSNHLPQLVLALILCGAGAALPWGLMDGLAISAVPVAKAGMAAGLFNTVRV 192
L + + ++ G + ++ + S++ +AG L N
Sbjct: 336 SFLTASFLLETTSWFM-TIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSF 394

Query: 193 AGEGIALAVVSAVLT 207
EG +A+V +L+
Sbjct: 395 LSEGTGIAIVGGLLS 409



Score = 28.3 bits (63), Expect = 0.036
Identities = 20/89 (22%), Positives = 37/89 (41%), Gaps = 3/89 (3%)

Query: 121 QVSTAGLMMASVGLLLLGD---AFHSNHLPQLVLALILCGAGAALPWGLMDGLAISAVPV 177
Q+ L++ + + G + L++A + GAGAA L+ + +P
Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK 134

Query: 178 AKAGMAAGLFNTVRVAGEGIALAVVSAVL 206
G A GL ++ GEG+ A+ +
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIA 163


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30050cloacin310.002 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 30.8 bits (69), Expect = 0.002
Identities = 15/44 (34%), Positives = 20/44 (45%)

Query: 23 PAYANPGNGNGNGGGNHGNSGNHGNSGNHGNNGNSGDHGNKGQN 66
+++ N G G G+ + G GN G NGNSG G N
Sbjct: 37 SGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80



Score = 29.7 bits (66), Expect = 0.006
Identities = 13/39 (33%), Positives = 16/39 (41%)

Query: 27 NPGNGNGNGGGNHGNSGNHGNSGNHGNNGNSGDHGNKGQ 65
NP G G + G HGN G +GN+G G
Sbjct: 44 NPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82



Score = 29.7 bits (66), Expect = 0.007
Identities = 11/32 (34%), Positives = 16/32 (50%)

Query: 26 ANPGNGNGNGGGNHGNSGNHGNSGNHGNNGNS 57
+ G G+G GN G +GN G G N ++
Sbjct: 52 SGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30040PF05272280.038 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.038
Identities = 16/49 (32%), Positives = 22/49 (44%), Gaps = 4/49 (8%)

Query: 33 VGGVSFNIRPGTIFG----LVGESGSGKTTVGRTLLGLYEKSAGSVKFH 77
+G V+ + PG F L G G GK+T+ TL+GL S
Sbjct: 582 MGHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIG 630


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_29970ARGREPRESSOR300.004 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 30.2 bits (68), Expect = 0.004
Identities = 14/44 (31%), Positives = 21/44 (47%), Gaps = 5/44 (11%)

Query: 10 QRQALICQILQENGRVVCAELAARLQ-----VSEHTIRRDLHEL 48
QR I +I+ N EL L+ V++ T+ RD+ EL
Sbjct: 5 QRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKEL 48


36KPHS_29500KPHS_29290Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_295002170.720953hypothetical protein
KPHS_294900133.079553endonuclease III
KPHS_294800143.472066NADH-ubiquinone oxidoreductase
KPHS_294701142.747214electron transport complex protein RnfG
KPHS_294601152.981710electron transport complex protein RnfD
KPHS_294502151.880764hypothetical protein
KPHS_294400140.079614electron transport complex protein
KPHS_29430115-1.161933electron transport complex protein RnfB
KPHS_29420013-0.209573Na(+)-translocating NADH-quinone reductase
KPHS_294100120.514530putative oxidoreductase
KPHS_294000130.011260oriC-binding nucleoid-associated protein
KPHS_293900140.5417406-phospho-beta-glucosidase
KPHS_293800140.989170putative oxidoreductase
KPHS_29370216-0.120206adenosine deaminase
KPHS_29360322-1.475521major facilitator family transporter
KPHS_29350018-1.665410putative transcriptional regulator
KPHS_293400141.057205hypothetical protein
KPHS_29330-1111.459943hypothetical protein
KPHS_29320-1111.527734cytochrome b561
KPHS_29310-1111.822700hypothetical protein
KPHS_29300-192.094127alcohol dehydrogenase class III
KPHS_29290-3103.119367glucose dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_29450IGASERPTASE393e-06 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 39.3 bits (91), Expect = 3e-06
Identities = 22/148 (14%), Positives = 44/148 (29%), Gaps = 10/148 (6%)

Query: 12 KAEQQAAPVDAPAAEPVDPRKAAVEAAIARAKARKAEQQAAPVDAPAAEPVDPRKAAVEA 71
+ E++ VD + +A V + + + +A PV P A +
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEA---------PVPPPAPATPS 1034

Query: 72 AIARAKARKAEQQAAPVDAPAVKPVDPRKAAVEAAIARAKARKAEQQATQQD-LASAAAN 130
A ++Q++ V+ + E A KA Q + S
Sbjct: 1035 ETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKE 1094

Query: 131 DDPRKAAVAAAIARVQARKATQQAVNEE 158
+ A + + + K + E
Sbjct: 1095 TQTTETKETATVEKEEKAKVETEKTQEV 1122



Score = 37.0 bits (85), Expect = 2e-05
Identities = 21/145 (14%), Positives = 43/145 (29%), Gaps = 10/145 (6%)

Query: 2 EAAIARAKARKAEQQAAPVDAPAAEPVDPRKAAVEAAIARAKARKAEQQAAPVDAPAAE- 60
+ A ++Q++ V+ + + E A KA Q V +E
Sbjct: 1033 PSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSET 1092

Query: 61 ----PVDPRKAAVEAAIARAKARKAEQQAAPVDAPAVKPVDPRKAAVEAAIARAKARKAE 116
+ ++ A +AK + Q P V P + V + +A A
Sbjct: 1093 KETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETV-----QPQAEPAR 1147

Query: 117 QQATQQDLASAAANDDPRKAAVAAA 141
+ ++ + + A
Sbjct: 1148 ENDPTVNIKEPQSQTNTTADTEQPA 1172



Score = 33.1 bits (75), Expect = 4e-04
Identities = 27/160 (16%), Positives = 48/160 (30%), Gaps = 13/160 (8%)

Query: 2 EAAIARAKARKAEQQAAPVDAPA-AEPVDPRKAAVEAAIARAKA-RKAEQQAAPVDAPAA 59
++ A APV PA A P + + E + +K K EQ A
Sbjct: 1007 VPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDAT------- 1059

Query: 60 EPVDPRKAAVEAAIARAKARKAEQQAAPVDAPAVKPVDPRKAAVEAAIARAKARKA-EQQ 118
+ E A KA Q V + + + + K KA +
Sbjct: 1060 ---ETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVET 1116

Query: 119 ATQQDLASAAANDDPRKAAVAAAIARVQARKATQQAVNEE 158
Q++ + P++ + + + VN +
Sbjct: 1117 EKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIK 1156



Score = 33.1 bits (75), Expect = 4e-04
Identities = 15/145 (10%), Positives = 40/145 (27%), Gaps = 7/145 (4%)

Query: 7 RAKARKAEQQAAPVDAPAAEPVDPRKAAVEAAIARAKARKAEQQAAPVDAPAAEPVDPRK 66
+AK + Q P P + V+ A+ + D +
Sbjct: 1111 KAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQ 1170

Query: 67 AAVEAAIARAKARKAEQQAAPVDAPAVKPVDPRKAAVE-------AAIARAKARKAEQQA 119
A E + + ++ P + A + + + + R++ +
Sbjct: 1171 PAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSV 1230

Query: 120 TQQDLASAAANDDPRKAAVAAAIAR 144
+ +++D A+ +
Sbjct: 1231 PHNVEPATTSSNDRSTVALCDLTST 1255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_29440IGASERPTASE397e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 38.5 bits (89), Expect = 7e-05
Identities = 24/143 (16%), Positives = 47/143 (32%), Gaps = 19/143 (13%)

Query: 437 RQEKAEIAAIRQEEQRAAEAKARFEA---------------RQARLEREKAARAERHKKA 481
+ E+ Q + A EAK+ +A E ++ A E+ +KA
Sbjct: 1053 KNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKA 1112

Query: 482 AVQPAAKDQEAISAALARVRDKQRDAAQPIVIQAGAKPDNSEAIAAREARKAEARARKAQ 541
V+ + + + +Q + QP QA +N + +E +++
Sbjct: 1113 KVETEKTQEVPKVTSQVSPKQEQSETVQP---QAEPARENDPTVNIKEP-QSQTNTTADT 1168

Query: 542 QQAAPMVAPAAEPVDPRKAAVEA 564
+Q A + E V
Sbjct: 1169 EQPAKETSSNVEQPVTESTTVNT 1191


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_29360TCRTETB432e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 42.6 bits (100), Expect = 2e-06
Identities = 35/146 (23%), Positives = 57/146 (39%), Gaps = 2/146 (1%)

Query: 28 LDSMAVSLKVSPGMIGSVITATQAGYAIGLLFLVPLGDWLNRKYVVMSQLLLSVAALVAA 87
L +A P V TA ++IG L D L K +++ ++++ V
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 88 GLSPNIVTLL-GAMLIVGLMAVVVQVLVAW-VAILATPQKRGQAVGTLTSGIVSGILLSR 145
+ + +LL A I G A LV VA + RG+A G + S + G +
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 146 FISGAIADIAGWRAVYLTAACLMLMI 171
I G IA W + L ++ +
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMITIITV 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_29330PF05272280.012 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 27.7 bits (61), Expect = 0.012
Identities = 8/35 (22%), Positives = 14/35 (40%)

Query: 83 QGVGSGQSDSALAGRDWHSPANLNDGAAASSMSLL 117
G+G + L G D+ S + + G S +
Sbjct: 605 GGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQI 639


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_29290TCRTETB300.044 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.8 bits (67), Expect = 0.044
Identities = 28/132 (21%), Positives = 48/132 (36%), Gaps = 27/132 (20%)

Query: 11 PRILQWLLAGLMLIIGLAVGILGAKLALVGGTLYFALMGVVMVIAAVLIFRNRRGGILLY 70
P W+ ML + + G + + I +L+F GI++
Sbjct: 48 PASTNWVNTAFMLTFSIGTAVYGK-------------LSDQLGIKRLLLF-----GIIIN 89

Query: 71 AVAFIASVIWAISDAGWNYWPLFSRLF-ALGVLAFLAALVWPFLAS--PPAKKGPAYGVA 127
SVI + + + + +R G AF ALV +A P +G A+G+
Sbjct: 90 C---FGSVIGFVGHSFF-SLLIMARFIQGAGAAAFP-ALVMVVVARYIPKENRGKAFGLI 144

Query: 128 AVLAVALAVSFG 139
+ VA+ G
Sbjct: 145 GSI-VAMGEGVG 155


37KPHS_28780KPHS_28700Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_28780-1143.328466hypothetical protein
KPHS_28770-2144.404110L-asparagine permease
KPHS_287600153.557677putative LysR-family transcriptional regulator
KPHS_287500153.259684LysR family transcriptional regulator
KPHS_287400143.381214hypothetical protein
KPHS_28730-1153.087916putative luciferase-like monooxygenase
KPHS_28720-1152.906456putative peptidase
KPHS_287100152.606722hypothetical protein
KPHS_287002194.023253putative acyltransferase
38KPHS_28610KPHS_28480Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_28610-1143.676833putative sugar hydrolase
KPHS_28600-1143.846478positive regulator for rhaBAD operon
KPHS_285900155.018394hypothetical protein
KPHS_285800154.715305putative ABC transport system substrate-binding
KPHS_285700153.759965putative ABC transporter substrate binding
KPHS_285600153.202552ABC transporter membrane protein
KPHS_28550-1141.008676N-hydroxyarylamine O-acetyltransferase
KPHS_28540-2130.282108respiratory nitrate reductase 2, gamma subunit
KPHS_28530-1130.481092cryptic nitrate reductase 2 subunit delta
KPHS_28520-2131.298427cryptic nitrate reductase 2 subunit beta
KPHS_28510-1121.675423cryptic nitrate reductase 2 subunit beta
KPHS_28500-1112.025906cryptic nitrate reductase 2 subunit alpha
KPHS_284901142.038638nitrate extrusion protein
KPHS_284802143.041882TetR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_28580PF05272300.007 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.007
Identities = 12/31 (38%), Positives = 14/31 (45%)

Query: 38 LLGPSGCGKSTLLRLLAGLSVPASGEIRFGD 68
L G G GKSTL+ L GL + G
Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIGT 631


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_28480HTHTETR521e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 51.9 bits (124), Expect = 1e-10
Identities = 26/180 (14%), Positives = 64/180 (35%), Gaps = 11/180 (6%)

Query: 5 QRDARREGIMQAAMRLALRGGFAAMTVRQIAREAQVAAGQLHHHFTSIGELKAQVFIRLI 64
+ R+ I+ A+RL + G ++ ++ +IA+ A V G ++ HF +L ++++
Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67

Query: 65 REMLDMPLVAED-------ASWRERL---FSMIGSEDGRLEPYIRLWREGQVLADSDPDI 114
+ ++ L + + RE L +E+ R + + +
Sbjct: 68 SNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERR-RLLMEIIFHKCEFVGEMAVV 126

Query: 115 KAAYLLTMNMWHAETVAIIEQGLASGEFRSAEPAADIAWRFIALVCGLDGIYALDAQALD 174
+ A + ++ + + + A + GL + Q+ D
Sbjct: 127 QQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFD 186


39KPHS_28360KPHS_28270Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_28360212-1.170234formate dehydrogenase-N alpha subunit
KPHS_28350014-0.776571formate dehydrogenase-N, Fe-S beta subunit,
KPHS_283400141.021975formate dehydrogenase-N gamma subunit
KPHS_28330-2133.268512hypothetical protein
KPHS_28320-1154.433404hypothetical protein
KPHS_28310-2145.769995putative glyoxalase/bleomycin resistance
KPHS_28300-1145.715938protein AraJ
KPHS_28290-1155.545068hypothetical protein
KPHS_28280-2144.705825putative monooxygenase
KPHS_28270-2133.791928ABC superfamily ATP binding cassette
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_28300TCRTETA574e-11 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 57.1 bits (138), Expect = 4e-11
Identities = 81/390 (20%), Positives = 135/390 (34%), Gaps = 37/390 (9%)

Query: 5 IFSLALGTFGLGMAEFGIMGVLPDMAHDVGISIPAA---GNMIAWYAFGVVIGAPIMALL 61
+ ++AL G+G+ IM VLP + D+ S G ++A YA AP++ L
Sbjct: 11 LSTVALDAVGIGL----IMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 62 SSRFSLKSVMLFLAGLCILGNTLFTFSSSYAMLALGRLVSGFPHGAFFGVGAIILSKIAP 121
S RF + V+L + + + +L +GR+V+G GA I
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIAD--IT 124

Query: 122 PGKVTAAVAGMIGGMTIANLVGVPGGTWLGHHFSWRYTFALIAVFNVAVFLAIFCWVPTL 181
G A G + +V P L FS F A N FL +P
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPES 184

Query: 182 YDRASTRLREQ---------FRFLASPAPWLI---FAATMFGNAGVFAWFSYIKPFMLNV 229
+ LR + + + L+ F + G W + +
Sbjct: 185 HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGE------ 238

Query: 230 SGFAESKMMLIMMLAGLGM---VVGNLLSGKISGRYSPLRIAAMTDGVIAVTLLLIFAFG 286
F + + LA G+ + +++G ++ R R + +L+
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFAT 298

Query: 287 EHKVASLALAFLCCAGLFALSAPLQILLLQNAKGGEMLGAAGGQIAF--NLGSAIGAFCG 344
+A + L G+ LQ +L + E G G +A +L S +G
Sbjct: 299 RGWMAFPIMVLLASGGIG--MPALQAMLSRQV-DEERQGQLQGSLAALTSLTSIVGPLLF 355

Query: 345 GMMIAQGFG-WNS-VALPAAALSFLAMSAL 372
+ A WN + AAL L + AL
Sbjct: 356 TAIYAASITTWNGWAWIAGAALYLLCLPAL 385


40KPHS_27910KPHS_27830Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_27910019-5.793440putative cation efflux system
KPHS_27900-122-8.121674hypothetical protein
KPHS_27890028-9.307442oxidoreductase alpha
KPHS_27880141-13.487149transcriptional regulator LysR
KPHS_27870145-14.448468cellobiose-specific PTS family enzyme IIC
KPHS_27860144-14.741868alpha-L-rhamnosidase
KPHS_27850140-12.543304putative helix-turn-helix AraC-type
KPHS_27840023-6.278755hypothetical protein
KPHS_27830019-3.753317hypothetical protein
41KPHS_27740KPHS_27520Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_277401153.845780putative LysR-family transcriptional regulator
KPHS_277302135.205082hypothetical protein
KPHS_277201135.251828hypothetical protein
KPHS_277100145.275619cell elongation, e phase, peptidoglycan
KPHS_277002146.522720glutamine amidotransferase
KPHS_276902165.828686coenzyme PQQ synthesis protein F
KPHS_276800153.233470coenzyme PQQ synthesis protein F
KPHS_27670-1142.191344pyrroloquinoline quinone biosynthesis protein
KPHS_276601151.926599pyrroloquinoline quinone synthesis protein D
KPHS_276501153.103527pyrroloquinoline quinone synthesis protein C
KPHS_276402153.850715pyrroloquinoline quinone synthesis protein B
KPHS_276303164.523847proline dipeptidase
KPHS_276202165.008639putative epoxide hydrolase protein
KPHS_276101134.424490OmpR family transcriptional regulatory protein
KPHS_27600-1134.120847hypothetical protein
KPHS_275900133.719744putative cytochrome c biogenesis protein
KPHS_275800133.866803LysR family NADH dehydrogenase transcriptional
KPHS_27570-1143.4773568-amino-7-oxononanoate synthase
KPHS_27560-3143.225153diaminobutyrate--2-oxoglutarate
KPHS_275500133.705661ABC transporter ATP-binding protein
KPHS_275400134.737246ABC transporter ATP-binding protein
KPHS_275300154.528012putative ABC transporter
KPHS_275200143.581145TENA/THI-4 family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27610HTHFIS964e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 96.4 bits (240), Expect = 4e-25
Identities = 42/127 (33%), Positives = 65/127 (51%), Gaps = 1/127 (0%)

Query: 6 HILVVDDDRDIRELIVDYLEKSGYRASGAANGKAMWSVLKNHQIDLIVLDIMMPGEDGLT 65
ILV DDD IR ++ L ++GY +N +W + DL+V D++MP E+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 66 LCRQLRANPQQDIPVLMLTARTDDSDRILGLEMGADDYLIKPFVARELLARIKAILRRTR 125
L +++ + D+PVL+++A+ I E GA DYL KPF EL+ I L +
Sbjct: 65 LLPRIKK-ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 126 ALPPNLQ 132
P L+
Sbjct: 124 RRPSKLE 130


42KPHS_27410KPHS_27090Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_27410220-2.244568urate catabolism protein
KPHS_27400034-5.759634hypothetical protein
KPHS_27390242-7.822860putative AraC-type regulatory protein
KPHS_27380247-10.172688hypothetical protein
KPHS_27370246-9.512648hypothetical protein
KPHS_27360346-10.008983phosphoglycerate mutase
KPHS_27350444-10.494899putative isochorismatase hydrolase
KPHS_27340440-9.155663putative deoxygluconate dehydrogenase
KPHS_27330121-2.552795putative glutamine amidotransferase class-I
KPHS_273201130.890613putative AraC-type regulatory protein
KPHS_273100122.367246hypothetical protein
KPHS_273000123.413847hypothetical protein
KPHS_27290-1123.733768hypothetical protein
KPHS_272800101.819981putative beta-ketoacyl synthase
KPHS_27270013-0.409307multidrug resistance protein membrane
KPHS_27260218-1.356202auxiliary transport protein, membrane fusion
KPHS_27250217-0.871250putative transcriptional regulator
KPHS_272401150.572063hypothetical protein
KPHS_272301141.125298putative aspartate-semialdehyde dehydrogenase
KPHS_272202176.742827acyltransferase domain-containing protein
KPHS_272104207.763564hypothetical protein
KPHS_272002197.063397amidohydrolase, AtzE family
KPHS_271901186.118487putative amidase
KPHS_271802195.301576hypothetical protein
KPHS_271701184.715305putative gamma-glutamyltransferase
KPHS_271602203.123752putative sugar isomerase
KPHS_271502204.649660cysteine ABC transporter periplasmic substrate
KPHS_271402185.145904glutamine ABC transporter permease
KPHS_271302184.981094amino acid ABC superfamily ATP binding cassette
KPHS_271201174.418371histidine transport
KPHS_271100175.214055Serine-pyruvate aminotransferase/ aspartate
KPHS_271000175.404726allantoate amidohydrolase
KPHS_270900163.623768putative dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27350ISCHRISMTASE572e-12 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 57.3 bits (138), Expect = 2e-12
Identities = 28/106 (26%), Positives = 50/106 (47%)

Query: 54 ADNESTAIHPDVAPAENEVVIVKRRVGAFSFTELEMILRAQGIENLILTGVTTSRVVLST 113
+ I ++AP ++++V+ K R AF T L ++R +G + LI+TG+ L T
Sbjct: 101 SGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVT 160

Query: 114 VGQAFDLDYRLIVVNDYCADPDPDTNMFLLKKVLPQHAFVTSSSEI 159
+AF D + V D AD + + L+ + AF + +
Sbjct: 161 ACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTVMTDSL 206


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27270TCRTETB979e-24 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 97.3 bits (242), Expect = 9e-24
Identities = 81/408 (19%), Positives = 157/408 (38%), Gaps = 21/408 (5%)

Query: 27 MCVGMFIALIDIQIVSASLRDIGGGLSAGDDETVWVQTSYLIAEIIIIPLSGWLARVMST 86
+C+ F ++++ +++ SL DI + T WV T++++ I + G L+ +
Sbjct: 19 LCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGI 78

Query: 87 RWLFAASAAGFTLMSLLCGWAWNIQS-MIAFRALQGLAGGSMIPLVFTTAFAFFQGKQRV 145
+ L S++ + S +I R +QG + LV + + R
Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138

Query: 146 IAAATIGGLASLAPTLGPTVGGWITENYNWHWLFFINVVPGIYIAVAVPLLVKVDSADPT 205
A IG + ++ +GP +GG I +W +L I ++ I + + LL K
Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGH 198

Query: 206 LLRGADYLSILLLALSLGCLEYTLEEGPRWGWFDDATLTTTAWVALLCGVAFVIRTLRHP 265
D I+L+++ + F + + V++L + FV +
Sbjct: 199 F----DIKGIILMSVGIVFFML----------FTTSYSISFLIVSVLSFLIFVKHIRKVT 244

Query: 266 QPVMDLRALQDRTFSLGCYFSFMAGVGIFATIYLTPLYLGSVRGFSALEIGLAV-FSTGL 324
P +D ++ F +G + + + + P + V S EIG + F +
Sbjct: 245 DPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTM 304

Query: 325 FQVMSIPFYSWLANRVDLRWLLMAGLIGFAVSMY--SFVPITHDWGADQLLLPQAFRGLA 382
++ L +R ++L G+ +VS SF+ T W +++ GL+
Sbjct: 305 SVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSW-FMTIIIVFVLGGLS 363

Query: 383 QQFAVAPTVTLTLGSLPPARLKLASGLFNLMRNLGGAIGIALCGTVLN 430
V T+ + SL L N L GIA+ G +L+
Sbjct: 364 FTKTVISTIVSS--SLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27260RTXTOXIND882e-21 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 88.0 bits (218), Expect = 2e-21
Identities = 41/284 (14%), Positives = 79/284 (27%), Gaps = 69/284 (24%)

Query: 44 VGGDISAISSKVSGYIQQLAVQDNMAVKKGDLLIRIDDRDYRAALAK------------- 90
G I + ++++ V++ +V+KGD+L+++ A K
Sbjct: 92 HSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQT 151

Query: 91 ----------------------------AAGEVAAQ-----------QAALADIQATRQL 111
+ EV Q +
Sbjct: 152 RYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDK 211

Query: 112 QQATIAGSAASLLAATAATEKLANDNRRYNALAASSAISAQIRDNASADYRRAHAEQEKA 171
++A A + + + +++L AI+ Y A E
Sbjct: 212 KRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVY 271

Query: 172 KADKTVAERQLAVLDARHQQ--------ILAALAQAQAN-------LEMARLNLSYTDIR 216
K+ E ++ +Q IL L Q N L + IR
Sbjct: 272 KSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIR 331

Query: 217 APFDGVIGNRRAWS-GSFVSSGTQLLSLVPA-HGLWIDANFKET 258
AP + + + G V++ L+ +VP L + A +
Sbjct: 332 APVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNK 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27220SACTRNSFRASE383e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.0 bits (88), Expect = 3e-06
Identities = 16/62 (25%), Positives = 27/62 (43%), Gaps = 1/62 (1%)

Query: 68 NRLWALISALVIEESSRGSGIGQQLLQAAERLARDKQCAQIELSSSEKRIRAHQFYENNG 127
N ALI + + + R G+G LL A A++ + L + + I A FY +
Sbjct: 87 NGY-ALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHH 145

Query: 128 YK 129
+
Sbjct: 146 FI 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27160BCTERIALGSPD290.020 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 29.1 bits (65), Expect = 0.020
Identities = 15/72 (20%), Positives = 27/72 (37%), Gaps = 13/72 (18%)

Query: 58 RLGYDKYKDMRDELRTL-------RQSGMPLTDQRDAV------QGNTLLARHYKQEMAN 104
L Y K D+ + L + +Q+ P+ + Q N L+ M +
Sbjct: 273 YLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMND 332

Query: 105 LTQWVNALDARQ 116
L + + LD R+
Sbjct: 333 LERVIAQLDIRR 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27120PF05272290.015 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.015
Identities = 13/42 (30%), Positives = 22/42 (52%), Gaps = 4/42 (9%)

Query: 31 VISIIGRSGSGKSTLLRCINGLEGYQEGSIKLGGMTITNRDS 72
+ + G G GKSTL+ + GL+ + + +G T +DS
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIG----TGKDS 635


43KPHS_27000KPHS_26490Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_27000-120-3.018087putative TetR/AcrR family transcriptional
KPHS_26990-124-4.436745hypothetical protein
KPHS_26980-124-4.806997putative acetyltransferase
KPHS_26970024-5.692021major facilitator superfamily protein
KPHS_26960032-9.028504hypothetical protein
KPHS_26950133-9.675613putative periplasmic binding protein/LacI
KPHS_26940136-10.930991PTS system protein
KPHS_26930133-9.484964PTS family protein II component
KPHS_26920233-8.134622PTS system ascorbate-specific transporter
KPHS_26910241-9.354698D-allulose-6-phosphate 3-epimerase
KPHS_26900245-8.998494PTS family enzyme IIA/ enzyme IIBC
KPHS_26890145-8.018229PTS system fructose-specific transporter subunit
KPHS_26880145-8.009115putative phosphotransferase system, EIIC
KPHS_26870146-7.710765amino acid exporter
KPHS_26860-239-5.735861MFS family transporter
KPHS_26850-230-3.382892LysR family transcriptional regulator
KPHS_26840-122-1.453861LysR family transcriptional regulator
KPHS_26830-117-0.170181N-acylhomoserine lactone degredation protein
KPHS_268200161.304751cell envelope integrity inner membrane protein
KPHS_26810-1161.425923putative acetylornithine deacetylase
KPHS_26800-1160.675258hypothetical protein
KPHS_26790-115-0.669222putative hydrolase
KPHS_26780014-0.711777inner-membrane translocator
KPHS_26770216-1.730619putative ABC transporter
KPHS_26760217-2.176539periplasmic binding protein/LacI transcriptional
KPHS_26750323-3.138548hypothetical protein
KPHS_26740224-3.568074putative ABC transporter, nucleotide
KPHS_26730021-3.494412carbohydrate kinase
KPHS_26720134-10.307838hypothetical protein
KPHS_26710037-12.452073hypothetical protein
KPHS_26700041-14.535975alpha/beta hydrolase-like protein
KPHS_26690-134-12.001412hypothetical protein
KPHS_26680-130-8.975663phosphoglycerate transporter
KPHS_26670-231-8.405095transposase
KPHS_26660-228-5.649658hypothetical protein
KPHS_26650-228-4.885841phosphoglycerate transporter protein
KPHS_26640-126-3.387629phosphoglycerate transporter
KPHS_26630-226-3.356971phosphoglycerate transporter
KPHS_26620-130-4.034041phosphoglycerate transport activator
KPHS_26610032-4.043825hypothetical protein
KPHS_26600031-3.061937LysR family transcriptional regulator
KPHS_26590129-3.039393putative aldo/keto reductase
KPHS_26580229-4.278843putative dehydrogenase
KPHS_26570035-5.763211hypothetical protein
KPHS_26560-129-4.389758gluconolactonase
KPHS_26550-131-5.122301oxidoreductase
KPHS_26540-129-6.157687hypothetical protein
KPHS_26530-129-6.525620hypothetical protein
KPHS_26520-131-6.109913glyceraldehyde-3-phosphate dehydrogenase
KPHS_26510-229-5.984795putative helix-turn-helix AraC-type
KPHS_26500-231-5.157152hypothetical protein
KPHS_26490-227-4.289074hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_27000HTHTETR681e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 68.5 bits (167), Expect = 1e-16
Identities = 31/171 (18%), Positives = 58/171 (33%), Gaps = 15/171 (8%)

Query: 2 KPKQADILRHASTLFNREGYQSPSIERIAEHAGISKMTFYRYYADKEALILAILKQKESE 61
+ + IL A LF+++G S S+ IA+ AG+++ Y ++ DK L I +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWEL-SES 68

Query: 62 FMQDLAQITADK------ASAREKLFAVFDYYHRWFTCETFHGCMFTRALFEYGASSPAI 115
+ +L K + RE L V + +F + F +
Sbjct: 69 NIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQ 128

Query: 116 REQCSRFKSLLWQFFRDILL------QVLKPEPAERVAMMMVMLIDGAIAA 160
++ + L + R A++M I G +
Sbjct: 129 AQRN--LCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMEN 177


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26970TCRTETA492e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 49.1 bits (117), Expect = 2e-08
Identities = 58/341 (17%), Positives = 107/341 (31%), Gaps = 40/341 (11%)

Query: 70 VTFSLLIILQTFFSPFQGRLVEKFGPRRLIAIGTVMAGMSWVLSAQVNGLATLWL---VY 126
+ +L ++Q +P G L ++FG R ++ + A + + + A L L++ V
Sbjct: 47 ILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVA 106

Query: 127 GCMGGLGTG----IVYIGVVGLMVKWFPQQRGFAAGAVAAGYGMGAIITTFPISLSLTTN 182
G G G I I + F GF + G G ++ S
Sbjct: 107 GITGATGAVAGAYIADITDGDERARHF----GFMSACFGFGMVAGPVLGGLMGGFSP--- 159

Query: 183 GLEHTMTTFGILFALVGFIASQ-GLKLPPPAVSQPVSQTVVQSSRSFTSREMLRQPLFWL 241
H + F+ L +P+ + + SF + + L
Sbjct: 160 ---HAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMT-VVAAL 215

Query: 242 MFVMMAMMSTSG-------LMVTSQMAVFAEDFGISQAVVFGMAALPLALTIDRFTNGLT 294
M V M + + A GIS A + +L A+
Sbjct: 216 MAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAM---------- 265

Query: 295 RPLFGFISDRFGREQTMFIAFALEGVAMMLWLACREDPLLFVLLSGVVFFGWGEIFSLFP 354
+ G ++ R G + + + +G +L + F ++ V+ G
Sbjct: 266 --ITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIM--VLLASGGIGMPALQ 321

Query: 355 STLTDTFGSEHAATNYGWLYISQGIGSIFGGPLAALLYQYT 395
+ L+ E G L + SI G L +Y +
Sbjct: 322 AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAAS 362



Score = 34.4 bits (79), Expect = 7e-04
Identities = 30/128 (23%), Positives = 55/128 (42%), Gaps = 6/128 (4%)

Query: 296 PLFGFISDRFGREQTMFIAFALEGVAMMLWLACREDPLLFVLLSGVVFFGW-GEIFSLFP 354
P+ G +SDRFGR + ++ A V + P L+VL G + G G ++
Sbjct: 61 PVLGALSDRFGRRPVLLVSLAGAAVDYAIMAT---APFLWVLYIGRIVAGITGATGAVAG 117

Query: 355 STLTDTFGSEHAATNYGWLYISQGIGSIFGGPLAALLYQYTHGWHVVFSCAIGLDFITAA 414
+ + D + A ++G++ G G + G L L+ ++ H F A L+ +
Sbjct: 118 AYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSP--HAPFFAAAALNGLNFL 175

Query: 415 LALWVLKP 422
++L
Sbjct: 176 TGCFLLPE 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26860TCRTETA310.013 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 30.6 bits (69), Expect = 0.013
Identities = 26/141 (18%), Positives = 48/141 (34%), Gaps = 4/141 (2%)

Query: 18 MVIAFVQFTNALEYMMFSPVFTFMAADF---AVPVTFSGYVSGMYTSGAVLSGIIAFYWI 74
+VI +A+ + PV + D G + +Y +
Sbjct: 8 IVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALS 67

Query: 75 DRCNKKHFLIANMVLLAMATLLTTFTTSFPLLLTLRFFAGLVGGTTMAVGITILINHTPA 134
DR ++ L+ ++ A+ + +L R AG+ G T AV + + T
Sbjct: 68 DRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGA-TGAVAGAYIADITDG 126

Query: 135 DLRGKMLATVIASFSMVSIVG 155
D R + + A F + G
Sbjct: 127 DERARHFGFMSACFGFGMVAG 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26830ALARACEMASE290.014 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 29.4 bits (66), Expect = 0.014
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 3/40 (7%)

Query: 41 LTHPD-GFTLIDGGLAVEGLKDPSGYWG-SAVEQFKPVMS 78
L HP+ F + G+ + G PSG W A +PVM+
Sbjct: 196 LWHPEAHFDWVRPGIILYGAS-PSGQWRDIANTGLRPVMT 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26770PF05272300.008 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.008
Identities = 14/43 (32%), Positives = 16/43 (37%), Gaps = 5/43 (11%)

Query: 26 VLGLVGDNGAGKSTLTKVLSGA-----VIPSSGTIRIDGEQQQ 63
+ L G G GKSTL L G GT + EQ
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIA 640


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26760HTHTETR290.022 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 28.8 bits (64), Expect = 0.022
Identities = 10/40 (25%), Positives = 21/40 (52%)

Query: 12 LSDVAKLAGLSKATLSRYMNNSIVLPQDTIDRIETAIREL 51
L ++AK AG+++ + + + L + + E+ I EL
Sbjct: 34 LGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGEL 73


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26690INTIMIN334e-04 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 32.7 bits (74), Expect = 4e-04
Identities = 22/70 (31%), Positives = 40/70 (57%), Gaps = 7/70 (10%)

Query: 84 SDGVKVTQSGAESR-FYTVKSGDTLSAISKAMYGSANEYQRIFEANKPMLTHPD---KIY 139
SD +T + ++R FYT+K+G+T++ +SK+ + + I+ NK + + K
Sbjct: 49 SDSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLST---IWSLNKHLYSSESEMMKAE 105

Query: 140 PGQVLIIPAK 149
PGQ +I+P K
Sbjct: 106 PGQQIILPLK 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26640FLGMOTORFLIM310.010 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 30.6 bits (69), Expect = 0.010
Identities = 5/35 (14%), Positives = 15/35 (42%), Gaps = 4/35 (11%)

Query: 312 QRLVQRMFDTAISFRLAQLKDAWRALHSAEVRLKR 346
+++ + LA ++++W + RL +
Sbjct: 150 NSVMEGVIVRI----LANVRESWTQVIDLRPRLGQ 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26620HTHFIS2451e-78 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 245 bits (627), Expect = 1e-78
Identities = 114/474 (24%), Positives = 194/474 (40%), Gaps = 73/474 (15%)

Query: 7 SILLIDDDADVLDAYTQLLEQAGYHVSACNNPFDAREQVPKDWPGIVLSDVCMPGCSGID 66
+IL+ DDDA + Q L +AGY V +N + +V++DV MP + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 67 LMTLFHQDDDLLPILLITGHGDVPMAVEAVKKGAWDFLQKPIDPGKLLTLVDAALRQRQS 126
L+ + LP+L+++ A++A +KGA+D+L KP D +L+ ++ AL + +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 127 VIARRQYCQQKLQVELIGRSQWTVRYRQRLQQLAETDIAVWLYGEPGTGRMTGARYLHQL 186
++ + Q L+GRS + L +L +TD+ + + GE GTG+ AR LH
Sbjct: 125 RPSKLEDDSQDGM-PLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY 183

Query: 187 GRHAEGPFIA--CELTPAN----------------AHTLNE-LIAQAQGGTLVLSHPEHL 227
G+ GPF+A P + A T + QA+GGTL L +
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 228 THEQQHQLVQ-LQSHEKRP----------FRLIGIGSASLVELAASSQIVAELYYCFAMT 276
+ Q +L++ LQ E R++ + L + +LYY +
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 277 QIGCQPLSKRPDDIEPLFHHYLQKTCQRLNHPVPEVDAGLLKGMMRRVWPNNVRELANAA 336
+ PL R +DI L H++Q+ + V D L+ M WP NVREL N
Sbjct: 304 PLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 337 ELFAV--------------------------------GVLPLAETVNPLMH--------- 355
G L +++ V M
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 356 IGEPTPLDQRVEDVERQIITEALNIHQGRINEVAEYLLIPRKKLYLRMKKYGLN 409
+ D+ + ++E +I AL +G + A+ L + R L ++++ G++
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


44KPHS_26360KPHS_25620Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_263602152.175729bifunctional putative acetyl-CoA:acetoacetyl-CoA
KPHS_263502151.1781923-oxoacyl-(acyl-carrier-protein) reductase
KPHS_263401132.072093putative acetyl-CoA acetyltransferase
KPHS_263300140.282710major facilitator family transporter
KPHS_26320-1131.208590major facilitator family transporter
KPHS_26310-1141.981460putative gluconolactonase
KPHS_263001142.171956putative general substrate transporter
KPHS_262902162.255210glycerate kinase
KPHS_262800150.705225putative sugar diacid utilization regulator
KPHS_26270-1171.5712216-phosphogluconate dehydrogenase
KPHS_26260-1150.623967transcriptional regulator LysR
KPHS_26250-212-1.634050hypothetical protein
KPHS_26240-213-3.395102putative transcriptional regulator
KPHS_26230-113-3.986431PTS family enzyme IIC/enzyme IIB
KPHS_26220-114-5.3105826-phospho-beta-glucosidase
KPHS_26210-121-7.925650hypothetical protein
KPHS_26200-118-5.818906major myo-inositol transporter
KPHS_26190029-6.482659phosphoenolpyruvate-dependent PTS family enzyme
KPHS_26180-220-4.267293hypothetical protein
KPHS_26170-217-4.029702hypothetical protein
KPHS_26160-318-3.878544hypothetical protein
KPHS_26150-216-3.364918hypothetical protein
KPHS_26140-116-4.152910esterase_lipase family protein IroE
KPHS_26130-118-4.602870hypothetical protein
KPHS_26120-119-4.185180hypothetical protein
KPHS_26110-115-0.339452hypothetical protein
KPHS_26100-1160.425583methionyl aminopeptidase
KPHS_26090-1171.318697hypothetical protein
KPHS_26080-1141.827263putative fimbrial-like protein
KPHS_26070-1142.598848putative fimbrial chaperone
KPHS_260600142.941756putative fimbrial biogenesis outer membrane
KPHS_26050-1143.188929fimbrial protein
KPHS_26040-2153.553314fimbrial protein
KPHS_26030-2173.162128hypothetical protein
KPHS_26020-1163.995513HipA family toxin-antitoxin system
KPHS_260100184.871414DNA-binding transcriptional regulator HipB
KPHS_260000195.160230transthyretin-like protein
KPHS_259902185.756557hypothetical protein
KPHS_259802175.783432putative guanine/xanthin permease
KPHS_259701186.710971putative flavoprotein monooxygenase
KPHS_259601186.502076transcriptional regulator LysR
KPHS_259501176.096148Rieske (2Fe-2S) domain-containing protein
KPHS_259400176.007195putative oxidoreductase
KPHS_25930-1154.931159purine ribonucleoside efflux pump NepI
KPHS_25920-2143.413971putative AraC-type regulatory protein
KPHS_25910-1142.553672hypothetical protein
KPHS_25900-1142.055209hypothetical protein
KPHS_25890-2112.626345hypothetical protein
KPHS_25880-2112.6778572OG-Fe(II) oxygenase
KPHS_258700152.985851D-methionine ABC superfamily ATP binding
KPHS_258602133.660804D- and L-methionine ABC transporter ATP-binding
KPHS_258502133.561925putative ABC-type metal ion transport system
KPHS_258402133.511177putative 2-nitropropane dioxygenase
KPHS_258302142.586506LysR family transcriptional regulator
KPHS_258202152.877184LysR family transcriptional regulator
KPHS_258101132.264703putative sugar transport protein
KPHS_25800-3151.556940prolyl endopeptidase
KPHS_25790017-0.634472hypothetical protein
KPHS_25780132-7.939534hypothetical protein
KPHS_25770033-7.516954hypothetical protein
KPHS_25760038-10.188169putative carboxymuconolactone decarboxylase
KPHS_25750027-6.846437hypothetical protein
KPHS_25740-125-6.164061hypothetical protein
KPHS_25730-219-3.877252hypothetical protein
KPHS_25720-313-0.221141hypothetical protein
KPHS_25710-2110.326228GNAT family acetyltransferase
KPHS_25700-2111.747568altronate oxidoreductase
KPHS_25690-2122.616803putative acyltransferase
KPHS_25680-2122.825136putative transport protein
KPHS_256700194.607989hypothetical protein
KPHS_256601194.356589glutaminase
KPHS_256500174.580761putative aldehyde dehydrogenase
KPHS_256402184.953349LysR family transcriptional regulator
KPHS_256303154.725307putative acyl-CoA N-acyltransferase
KPHS_256201154.562645putative malate dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26350DHBDHDRGNASE1241e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (311), Expect = 1e-36
Identities = 74/254 (29%), Positives = 121/254 (47%), Gaps = 12/254 (4%)

Query: 3 LASKTAIVTGAARGIGFGIAQVLAREGARVIIADRDAHG-EAAAASLRESGAQALFISCN 61
+ K A +TGAA+GIG +A+ LA +GA + D + E +SL+ A +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 62 IAEKTQVEALFSQAEEAFGPVDILVNNAGINRDAMLHKLTEADWDTVIDVNLKGTFLCMQ 121
+ + ++ + ++ E GP+DILVN AG+ R ++H L++ +W+ VN G F +
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 122 QAAIRMRERGAGRIINIAS-ASWLGNVGQTNYSASKAGVVGMTKTACRELAKKGVTVNAI 180
+ M +R +G I+ + S + + Y++SKA V TK ELA+ + N +
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 181 CPGFIDTDMTRG--VPENVWQIMIS--------KIPAGYAGEAKDVGECVAFLASDGARY 230
PG +TDM EN + +I IP + D+ + V FL S A +
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 231 INGEVINVGGGMVL 244
I + V GG L
Sbjct: 246 ITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26330VACCYTOTOXIN260.045 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 26.1 bits (57), Expect = 0.045
Identities = 10/24 (41%), Positives = 18/24 (75%)

Query: 13 QLQRTHKKIYRHLMPLLIVAYIIS 36
++Q+TH+KI R L+ L +V ++S
Sbjct: 2 EIQQTHRKINRPLVSLALVGALVS 25


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26320TCRTETB300.016 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.8 bits (67), Expect = 0.016
Identities = 26/157 (16%), Positives = 48/157 (30%), Gaps = 13/157 (8%)

Query: 131 STRVFLLLVLIYFTHQFSVYGLSYFLPGIIGSWGQLTPLQVGLLTAIP-----WIAAAAG 185
F++ VL +V G +P ++ QL+ ++G + P I G
Sbjct: 254 KNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIG 313

Query: 186 GILLPRFARTEQRSRSMLMAGYLVMATGMAIGAIAGHG---VALLGFSLAAFMFFAMQSI 242
GIL+ R +L G ++ + + +++
Sbjct: 314 GILVDRRG-----PLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTV 368

Query: 243 IFNWLPSIMSGHMLAGSFGLLNCLGLCGGFLGPFILG 279
I + S + LLN G I+G
Sbjct: 369 ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVG 405


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26300TCRTETB392e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 39.5 bits (92), Expect = 2e-05
Identities = 71/399 (17%), Positives = 134/399 (33%), Gaps = 51/399 (12%)

Query: 53 EMILRLG-PVISKEFSLSPEQWGNIVALIMVALAVLDIPGSIWSDRYGSGWKRARFQVPL 111
EM+L + P I+ +F+ P + M+ ++ SD+ G + L
Sbjct: 30 EMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLG-------IKRLL 82

Query: 112 VLGYTALSFISGIKAISHGLTAFVLL-RVGVNLGAGWGEPVGVSNTAEWWPKEKRGFALG 170
+ G F S I + H + +++ R GA + + A + PKE RG A G
Sbjct: 83 LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFG 142

Query: 171 VHHTGYPIGALLSGVVASLVLATFGEGSWRYCFLL--ALLVAIPLMIFWAKYSTADRINT 228
+ + +G + + ++ W Y L+ ++ +P ++ K RI
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIH---WSYLLLIPMITIITVPFLMKLLK--KEVRIK- 196

Query: 229 LYQHIDSQG----------LTRPATQES---------------SHVAKGEGMKTFLRTLR 263
H D +G T S H+ K +
Sbjct: 197 --GHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGK 254

Query: 264 NRNISLTAGNTLLTQIVYMGINVVLPPYLYHVSGLSLAASAGLSIIF--TLTGTLGQVIW 321
N + + G ++P + V LS A G IIF T++ + I
Sbjct: 255 NIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAE-IGSVIIFPGTMSVIIFGYIG 313

Query: 322 PWLSDSFGRKRTLIVCGLWMSIG---IALFYFATNMPRLIAIQLFFGLVANAVWPIYYAM 378
L D G L + ++S+ + T+ I I G ++ + +
Sbjct: 314 GILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLS-FTKTVISTI 372

Query: 379 ASDSAEERATSTANGIITTAMFIGGGISPLLMGWLIQFG 417
S S +++ ++ F+ G ++G L+
Sbjct: 373 VSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIP 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26280DNABINDNGFIS290.012 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 28.8 bits (64), Expect = 0.012
Identities = 13/50 (26%), Positives = 30/50 (60%), Gaps = 2/50 (4%)

Query: 296 INNVRQLLEHDSGEVLLDTLSSFIANNAEPGKTSLLLGIHRNTLTYRLQQ 345
+N++ +L+ + + LLD + + N + +L++GI+R TL +L++
Sbjct: 47 VNDLYELVLAEVEQPLLDMVMQYTRGNQT--RAALMMGINRGTLRKKLKK 94


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26200TCRTETA402e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 39.8 bits (93), Expect = 2e-05
Identities = 26/118 (22%), Positives = 48/118 (40%), Gaps = 1/118 (0%)

Query: 55 GLVMSVLLVGAALGSVFGGKFADYFGRRKYLLFLSFVFLIGALLSAAAPDITTLLIARAL 114
G+++++ + + G +D FGRR LL + + A AP + L I R +
Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105

Query: 115 LGYAVGGASVTAPTFISEVAPTEMRGKLTGLNEVAIVIGQLAAFAINAIIGIIWGHLP 172
G G A +I+++ + R + G G +A + ++G H P
Sbjct: 106 AGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAP 162



Score = 32.5 bits (74), Expect = 0.004
Identities = 32/137 (23%), Positives = 51/137 (37%), Gaps = 22/137 (16%)

Query: 321 LVDRFKRKTIIIYGFAIMATLHLIIAAVDYTLVGDLKATAIWLLGALFVGVMQGSMGFIT 380
L DRF R+ +++ L AAVDY ++ + +G + G + G+ G +
Sbjct: 66 LSDRFGRRPVLLVS--------LAGAAVDYAIMATAPFLWVLYIGRIVAG-ITGATGAVA 116

Query: 381 WVVLAELFPLKFRGLSMGISVFFMWIMNAVVSYLFPL------LQAKLGLGPVFFIFAAI 434
+A++ R G M+A + L FF AA+
Sbjct: 117 GAYIADITDGDERARHFGF-------MSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAAL 169

Query: 435 NYLAILFVVFALPETSN 451
N L L F LPE+
Sbjct: 170 NGLNFLTGCFLLPESHK 186



Score = 30.2 bits (68), Expect = 0.018
Identities = 30/152 (19%), Positives = 51/152 (33%), Gaps = 8/152 (5%)

Query: 48 ALTPTTEGLVMSVL-LVGAALGSVFGGKFADYFGRRKYLLFLSFVFLIGALLSAAAPDIT 106
TT G+ ++ ++ + ++ G A G R+ L+ G +L A A
Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGW 301

Query: 107 TLLIARALLGYAVGGASVTAPTFISEVAPTEMRGKLTGLN----EVAIVIGQLAAFAINA 162
LL + G +S E +G+L G + ++G L AI A
Sbjct: 302 MAFPIMVLLA-SGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYA 360

Query: 163 IIGIIWGHLPDVWRYMLLVQAIPAICLFVGMW 194
W W + + L G+W
Sbjct: 361 ASITTWNGW--AWIAGAALYLLCLPALRRGLW 390


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26090FIMBRIALPAPE270.013 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 26.5 bits (58), Expect = 0.013
Identities = 12/35 (34%), Positives = 18/35 (51%), Gaps = 6/35 (17%)

Query: 11 LCLAPLASSAALSGQVH------FSGRVINPACVI 39
LCL + + +S VH F G++I PAC +
Sbjct: 7 LCLPVMLGAVLMSQHVHAADNLTFKGKLIIPACTV 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26080FIMBRIALPAPE334e-04 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 32.7 bits (74), Expect = 4e-04
Identities = 40/168 (23%), Positives = 75/168 (44%), Gaps = 22/168 (13%)

Query: 24 ARAAGTLNFTGKIINESCQIANNGGDVNVDFGNVDMSALKSHEAKTAETPFTINLTGCPL 83
AA L F GK+I +C + N V++G++++ L ++ + FT+++ CP
Sbjct: 22 VHAADNLTFKGKLIIPACTVQN----AEVNWGDIEIQNLV--QSGGNQKDFTVDMN-CPY 74

Query: 84 AQNISISLEGTPDTNANGTSAAVLALSDSADTAKGVGIEVFSSPDGS-----TEGTQLTF 138
S+ T+ T ++L + S + G+ I +++S + T G+Q+T
Sbjct: 75 ----SLGTMKVTITSNGQTGNSILVPNTSTASGDGLLIYLYNSNNSGIGNAVTLGSQVTP 130

Query: 139 DKQSKTAVSQADENGDIAFNFIADVKSDSSQDVTAGNINATANIDIVY 186
K + TA ++ I K + Q + AG +ATA + Y
Sbjct: 131 GKITGTAPAR-----KITLYAKLGYKGN-MQSLQAGTFSATATLVASY 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26060PF005776800.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 680 bits (1757), Expect = 0.0
Identities = 311/861 (36%), Positives = 451/861 (52%), Gaps = 52/861 (6%)

Query: 12 VSLSILLGGQSALLHAQAT--FNMDLLEKNDHLPAVDLQRFNQQAGQPPGAYPVSWQVNG 69
V L + + + A FN L + DL RF PPG Y V +N
Sbjct: 28 VRLFVACAFAAQAPLSSAELYFNPRFLADDP-QAVADLSRFENGQELPPGTYRVDIYLNN 86

Query: 70 VTLDARKTVTFRQND-RGQLTPCLKPEDLLQAGVNPAVLSQAPSATSRSCPELNALLPGS 128
+ + VTF D + PCL L G+N A +S +C L +++ +
Sbjct: 87 GYMA-TRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDA 145

Query: 129 TVNFDFAHQRLVMTIPQALMTHRARDNVPSALWDEGISAFQSNYRYSGASQRTREGSTER 188
T D QRL +TIPQA M++RAR +P LWD GI+A NY +SG S + R G
Sbjct: 146 TAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSH 205

Query: 189 DNYLMLKSGVNLGAWRLRASNSLTAN-----SDDKPQWTTSGAWLERDLTRWQSELTLGD 243
YL L+SG+N+GAWRLR + + + N S K +W WLERD+ +S LTLGD
Sbjct: 206 YAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGD 265

Query: 244 TFTSGDVFDAVQFQGISLASSDAMLPDSQKGFAPTIRGIARTNAQVTVRQNGYVLYQTYV 303
+T GD+FD + F+G LAS D MLPDSQ+GFAP I GIAR AQVT++QNGY +Y + V
Sbjct: 266 GYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTV 325

Query: 304 TPGAFVIDDLYPTASSGNLEVAVKESDGEIRRFTQPYASVTSMQREGSLKYNLVAGRYHS 363
PG F I+D+Y +SG+L+V +KE+DG + FT PY+SV +QREG +Y++ AG Y S
Sbjct: 326 PPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRS 385

Query: 364 DDASQR-PLMMQLSLMRGFAHNLTLFGGLQSAAQYHNLSLGAGQGLGEAGALSLQLLNAR 422
+A Q P Q +L+ G T++GG Q A +Y + G G+ +G GALS+ + A
Sbjct: 386 GNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQAN 445

Query: 423 DR-HQQDPIDGRAWQLQYSKGFDRLGTQLTFTGWRYSHQRYATLSEAFSSPGSDDDLQDS 481
DG++ + Y+K + GT + G+RYS Y ++ S + +++
Sbjct: 446 STLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQ 505

Query: 482 D-----------------NKKATLQITASQSLPYDITLYLSLDQDSYWSGGATQRTANMG 524
D NK+ LQ+T +Q L TLYLS +YW G
Sbjct: 506 DGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAG 565

Query: 525 ISSRVHGIAWSLSYSDSRSSHGDEEDDEPHSDKVVTLSLSVPLSHLLPG--------SYA 576
+++ I W+LSYS ++++ D+++ L++++P SH L + A
Sbjct: 566 LNTAFEDINWTLSYSLTKNAWQKG------RDQMLALNVNIPFSHWLRSDSKSQWRHASA 619

Query: 577 GYTLTSSRHSVGSQMVSLNGTLLDNHALSYAVSQTRDRQ----NGSSGSLTAGYSSGRGD 632
Y+++ + + + + GTLL+++ LSY+V +GS+G T Y G G+
Sbjct: 620 SYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGN 679

Query: 633 LNLGYSHDSQAARLNYGASGGILIHRHGVVFTPEMNGAVVLIDAGGAGGVTLANQKTIAT 692
N+GYSH +L YG SGG+L H +GV +N VVL+ A GA + NQ + T
Sbjct: 680 ANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRT 739

Query: 693 NGDGYAVLPFATAYHRNDVSLDSHSLPENVDLANSTVTLVPTEDAVVLARFHTHVGYKAL 752
+ GYAVLP+AT Y N V+LD+++L +NVDL N+ +VPT A+V A F VG K L
Sbjct: 740 DWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLL 799

Query: 753 FTLQSRGQPLPFGSEVRAKDTNS--IVASEGQVYLAGLAPKGTLYAQWGPGPQQRCSARY 810
TL +PLPFG+ V ++ + S IVA GQVYL+G+ G + +WG C A Y
Sbjct: 800 MTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANY 859

Query: 811 DLTPTLAQTPHPLILQQTLSC 831
L P Q + Q + C
Sbjct: 860 QLPPESQQQL---LTQLSAEC 877


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25930TCRTETB441e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 43.7 bits (103), Expect = 1e-06
Identities = 31/157 (19%), Positives = 59/157 (37%), Gaps = 2/157 (1%)

Query: 26 LGVFGLIVAEFLPASLLTPMASSLGVSEGMAGQAVTATALVALVTGLLIATATRNIDRRW 85
L F ++ L SL +A+ TA L + + + + +
Sbjct: 22 LSFFSVLNEMVLNVSLPD-IANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKR 80

Query: 86 VLMFFSVLQIVSSLMVAFADSLAFLL-LGRLLLGIAIGGFWAMSTATAMRLVPAAHVPKA 144
+L+F ++ S++ S LL + R + G F A+ R +P + KA
Sbjct: 81 LLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKA 140

Query: 145 LAIIFSAVSVATVVAAPLGSYLGELIGWRNVFILCAI 181
+I S V++ V +G + I W + ++ I
Sbjct: 141 FGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMI 177


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25890MICOLLPTASE260.025 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 25.8 bits (56), Expect = 0.025
Identities = 10/29 (34%), Positives = 19/29 (65%)

Query: 52 RRTPWARKEVEAMYLASLDDDAPVEKADP 80
+R WA KEV+A ++ + +D +E+ +P
Sbjct: 415 KRLYWASKEVKAQFMRVVQNDKALEEGNP 443


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25810TCRTETA310.006 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 31.3 bits (71), Expect = 0.006
Identities = 31/128 (24%), Positives = 50/128 (39%), Gaps = 4/128 (3%)

Query: 246 VHLWALFGLAAAPSCLIWHKLVLKWGYRQALTRNLLVQALGVILPACSASLLFCVLSALL 305
+ L+AL A AP + L ++G R L +L A+ + A + L + ++
Sbjct: 49 LALYALMQFACAP---VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105

Query: 306 VGFTFMGTVTIALPKAKSLSHQVSFNMIAAMTALYGVGQIAGPLIAGALYQIAASFNPAL 365
G T A M+A +G G +AGP++ G + + P
Sbjct: 106 AGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHA-PFF 164

Query: 366 YAAALALL 373
AAAL L
Sbjct: 165 AAAALNGL 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25790TCRTETA415e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 41.3 bits (97), Expect = 5e-06
Identities = 81/397 (20%), Positives = 143/397 (36%), Gaps = 32/397 (8%)

Query: 11 NLRIISIVVFTCICYLSIGLPLAVLPGYIHYQLGYSTFVA---GIVISLQYISTLISRPH 67
N +I I+ + + IGL + VLPG + L +S V GI+++L + P
Sbjct: 4 NRPLIVILSTVALDAVGIGLIMPVLPGLLR-DLVHSNDVTAHYGILLALYALMQFACAPV 62

Query: 68 AGRYTDIWGPKKVVSLGIVCCLLSGAFTLLAVVLQATPMLAIAALLAGRVFLGV-GESFT 126
G +D +G + V+ + + + ++ P L + L GR+ G+ G +
Sbjct: 63 LGALSDRFGRRPVLLVSLA------GAAVDYAIMATAPFLWV--LYIGRIVAGITGATGA 114

Query: 127 ATGATLWGIKTVGAIHTSRVISWNGVATYVAMAVGAPLGVTLNHYFGISGF--ATVVVLV 184
GA + I +R + M G LG + + + F A + +
Sbjct: 115 VAGAYIADITDGDE--RARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGL 172

Query: 185 AAIGLLF-------ARTRQDVKVNAGARAPFH-AVVRKIWPYGLGLAFGTVGFGVIATFI 236
+ F R + A F A + + + F G + +
Sbjct: 173 NFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAAL 232

Query: 237 TLYFAAHSWQ----GAAFTLSLFSVGFICVRLVLGNTIT-RFGGVPVSLACFIIESLGLL 291
+ F + +L+ F + + ++ + R G + I + G +
Sbjct: 233 WVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYI 292

Query: 292 LIWLAPSAWMAGVGAFLTGSGFSLVFPALGVEAVKQVEEQNQGTALGTYSAFLDLALGLT 351
L+ A WMA L SG + PAL +QV+E+ QG G+ +A L +
Sbjct: 293 LLAFATRGWMAFPIMVLLASG-GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT-SIV 350

Query: 352 GPLAGWVAGFYDLATLYLLAAIVVALAFLLIFRVHRQ 388
GPL + T A I A +LL R+
Sbjct: 351 GPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25710SACTRNSFRASE316e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 31.5 bits (71), Expect = 6e-04
Identities = 11/51 (21%), Positives = 21/51 (41%), Gaps = 1/51 (1%)

Query: 81 YLEDLFVDPAFRGQGIARTMIKSLQSEGADKGWSRLYWHTRRDN-PARHLY 130
+ED+ V +R +G+ ++ + + L T+ N A H Y
Sbjct: 91 LIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFY 141


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25690SACTRNSFRASE327e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 31.8 bits (72), Expect = 7e-04
Identities = 21/86 (24%), Positives = 39/86 (45%), Gaps = 4/86 (4%)

Query: 92 RADVAKLLVHQNVRRQGIAQALMSELERIARRERKTVLVLDTAT-GSGAEQFYARCGWEK 150
A + + V ++ R++G+ AL+ + A+ L+L+T A FYA+ +
Sbjct: 89 YALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF-I 147

Query: 151 VGEIPR--YALMPDGEMTATSLFYKF 174
+G + Y+ P A +YKF
Sbjct: 148 IGAVDTMLYSNFPTANEIAIFWYYKF 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25660BLACTAMASEA353e-04 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 34.8 bits (80), Expect = 3e-04
Identities = 15/71 (21%), Positives = 31/71 (43%), Gaps = 3/71 (4%)

Query: 22 GRGKVADYIPALASVSGDKLGI-AISTVDGQHFAAGDAHERFSIQSISKVL--SLVVAMN 78
+ + I S ++G+ + G+ A A ERF + S KV+ V+A
Sbjct: 21 ASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARV 80

Query: 79 HYQEEEIWQRV 89
+E++ +++
Sbjct: 81 DAGDEQLERKI 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25630SACTRNSFRASE387e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 37.6 bits (87), Expect = 7e-06
Identities = 14/73 (19%), Positives = 25/73 (34%), Gaps = 1/73 (1%)

Query: 80 IDPQHRGQQLGEKLLAALEAKARQRDCHTLRLETGIHQHAAIALYTRNGYQTRCAFAPYQ 139
+ +R + +G LL A++ L LET +A Y ++ + A
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF-IIGAVDTML 155

Query: 140 PDPLSVFMEKPLF 152
E +F
Sbjct: 156 YSNFPTANEIAIF 168


45KPHS_25500KPHS_25080Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_25500015-3.114312multiple drug resistance protein MarC
KPHS_25490114-1.060232hypothetical protein
KPHS_254801130.385922DNA-binding transcriptional repressor MarR
KPHS_254703161.733444DNA-binding transcriptional activator MarA
KPHS_254603172.958982hypothetical protein
KPHS_254503183.937178O-acetylserine/cysteine export protein
KPHS_254403174.755063putative transport protein
KPHS_254301174.034148DNA-binding protein
KPHS_254200163.862970putative ABC transport ATP-binding subunit
KPHS_2541009-1.337120urea ABC transporter ATP-binding protein UrtD
KPHS_25400011-2.557702high-affinity branched-chain amino acid ABC
KPHS_25390-113-3.490240high-affinity branched-chain amino acid ABC
KPHS_25380017-4.421370urea ABC transporter, urea binding protein
KPHS_25370021-5.5044795-
KPHS_25360028-7.672745transposase
KPHS_253500200.857833hypothetical protein
KPHS_253402184.499477hypothetical protein
KPHS_253300164.417428transcriptional activator FtrA
KPHS_253201154.704196hypothetical protein
KPHS_253101154.483418hypothetical protein
KPHS_25300-1133.772412hypothetical protein
KPHS_25290-2133.608420lac repressor
KPHS_25280-2122.480852beta-D-galactosidase
KPHS_25270-2122.128359beta-D-galactosidase
KPHS_25260-1140.483612hypothetical protein
KPHS_25250-1131.868811putative proton/sugar symporter, LacY
KPHS_25240-1153.827495putative RecF protein
KPHS_25230-1154.462041putative K+ transporting ATPase, KdpC subunit
KPHS_25220-1164.011145beta-lactamase SHV-11
KPHS_252100153.384347DeoR family transcriptional regulator
KPHS_25200-2132.440972putative dehydrogenase
KPHS_25190-2150.516426ygbK domain protein
KPHS_25180-217-1.032763putative aldolase
KPHS_25170-216-1.028915putative epimerase/isomerase
KPHS_25160-217-1.929341putative transport protein
KPHS_25150-120-3.258100putative ARAC-type regulatory protein
KPHS_25140023-3.868969LuxR transcriptional regulator
KPHS_25130-125-5.099799hypothetical protein
KPHS_25120137-9.581301competence damage-inducible protein A
KPHS_25110146-14.929493heat shock protein
KPHS_25100340-10.857250hypothetical protein
KPHS_25090443-11.545011hypothetical protein
KPHS_25080022-3.426541putative 2-component transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25440TCRTETA462e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 46.0 bits (109), Expect = 2e-07
Identities = 46/237 (19%), Positives = 89/237 (37%), Gaps = 14/237 (5%)

Query: 7 RSTIALLASSLLLTIGRGATLPFMTIYLTRRFQLEVDVI---GYALSLALVVGVLFSMGF 63
R I +L++ L +G G +P + L R DV G L+L ++ +
Sbjct: 5 RPLIVILSTVALDAVGIGLIMPVLPGLL-RDLVHSNDVTAHYGILLALYALMQFACAPVL 63

Query: 64 GILADRFDKKRYMVWSVLVFILGFSAIPLVNNAPLVVIFFA--LINCAYSVFSTVLKAWF 121
G L+DRF ++ ++ S+ + ++ ++ AP + + + ++ V A+
Sbjct: 64 GALSDRFGRRPVLLVSLAGAAVDYA---IMATAPFLWVLYIGRIVAGITGATGAVAGAYI 120

Query: 122 ADRLTAEKKARIFSLNYTILNIGWTVGPPIGTLLVMHSINLPFWLAAACAAFPLVFIQLF 181
AD +++AR F G GP +G L+ S + PF+ AAA +
Sbjct: 121 ADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFL 180

Query: 182 L----QRDGAAAAQPGAAPWSPSVLLRD-RALLWFTCSGLLASFVGGAFASCLSQYV 233
L + + + P + R + + VG A+ +
Sbjct: 181 LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG 237



Score = 39.4 bits (92), Expect = 2e-05
Identities = 26/158 (16%), Positives = 62/158 (39%), Gaps = 2/158 (1%)

Query: 4 TLRRSTIALLASSLLLTIGRGATLPFMTIYLTRRFQLEVDVIGYALSLALVVGVLF-SMG 62
AL+A ++ + I+ RF + IG +L+ ++ L +M
Sbjct: 207 RGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMI 266

Query: 63 FGILADRFDKKRYMVWSVLVFILGFSAIPLVNNAPLVVIFFALINCAYSVFSTVLKAWFA 122
G +A R ++R ++ ++ G+ + + L+ + L+A +
Sbjct: 267 TGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASG-GIGMPALQAMLS 325

Query: 123 DRLTAEKKARIFSLNYTILNIGWTVGPPIGTLLVMHSI 160
++ E++ ++ + ++ VGP + T + SI
Sbjct: 326 RQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASI 363


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25430TYPE3OMBPROT280.021 Type III secretion system outer membrane B protein ...
		>TYPE3OMBPROT#Type III secretion system outer membrane B protein

family signature.
Length = 538

Score = 27.7 bits (61), Expect = 0.021
Identities = 14/32 (43%), Positives = 22/32 (68%), Gaps = 1/32 (3%)

Query: 19 EQLAEMAGLSVRTIQRIENGER-PGLETLSAL 49
E+ + G+SV + QR++NGER G+E L+ L
Sbjct: 23 EETGKHKGVSVISYQRVKNGERNKGIEALNRL 54


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25410PF05211280.039 Neuraminyllactose-binding hemagglutinin
		>PF05211#Neuraminyllactose-binding hemagglutinin

Length = 260

Score = 27.7 bits (61), Expect = 0.039
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 7/55 (12%)

Query: 43 LSLAIGVGELRCVIGPNGAGKTTLMDVITGKTRPQSGKALYDQSVDLTTLDPVAI 97
L + G+ ++ V+ P G K T+++ P SG++L ++DL+ LD
Sbjct: 145 LLFSTGLDKMEGVLIPAGFVKVTILE-------PMSGESLDSFTMDLSELDIQEK 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25250TCRTETA392e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 39.4 bits (92), Expect = 2e-05
Identities = 58/271 (21%), Positives = 104/271 (38%), Gaps = 37/271 (13%)

Query: 12 TETGIVFSSISLFAIIFQPVFGLMSDKLGLRKHLLWTITVLLILFA-PFFIFVFSPLLQM 70
GI+ + +L PV G +SD+ G R LL V L A + I +P L +
Sbjct: 43 AHYGILLALYALMQFACAPVLGALSDRFGRRPVLL----VSLAGAAVDYAIMATAPFLWV 98

Query: 71 NIIAGSLVGGIYLGIVFSSGSGAVEAYIERVSRANRFEYGKVRVAGCVGWALCAS--ITG 128
+ G +V GI G + + + RA F + ++ C G+ + A + G
Sbjct: 99 -LYIGRIVAGI-TGATGAVAGAYIADITDGDERARHFGF----MSACFGFGMVAGPVLGG 152

Query: 129 VLFSIDPNITFWIASGFALVLGLLLWLSRPESSNS------AQVIEALGANRQAFSLRTA 182
++ P+ F+ A+ + L PES + + L + R A +
Sbjct: 153 LMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVV 212

Query: 183 AELLRMPRFWGFIVYVVG--VASVYDVFDQQFANFFKSFFASPQRGTEVFGFVTTGGELL 240
A L+ FI+ +VG A+++ +F + ++ G +L
Sbjct: 213 AALM----AVFFIMQLVGQVPAALWVIFGEDRFHW----------DATTIGISLAAFGIL 258

Query: 241 NALI-MFCAPAIVNRIGAKNALLTAGMIMSV 270
++L + R+G + AL+ GMI
Sbjct: 259 HSLAQAMITGPVAARLGERRALM-LGMIADG 288


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_25220BLACTAMASEA437e-159 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 437 bits (1126), Expect = e-159
Identities = 285/286 (99%), Positives = 285/286 (99%)

Query: 1 MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIEMDLASGRTLTAWRADE 60
MRYIRLCIISLLATLPLAVHASPQPLEQIK SESQLSGRVGMIEMDLASGRTLTAWRADE
Sbjct: 1 MRYIRLCIISLLATLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADE 60

Query: 61 RFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCA 120
RFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCA
Sbjct: 61 RFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCA 120

Query: 121 AAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTTPA 180
AAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTTPA
Sbjct: 121 AAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTTPA 180

Query: 181 SMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLPAGWFIADKTGAGERGARG 240
SMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLPAGWFIADKTGAGERGARG
Sbjct: 181 SMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLPAGWFIADKTGAGERGARG 240

Query: 241 IVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALIEHWQR 286
IVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALIEHWQR
Sbjct: 241 IVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALIEHWQR 286


46KPHS_24910KPHS_24660Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_249102133.734827anaerobic dimethyl sulfoxide reductase subunit
KPHS_249003132.579717putative dimethyl sulfoxide reductase, anchor
KPHS_248902122.662578putative dimethyl sulfoxide reductase, anchor
KPHS_248801122.642313putative dimethyl sulfoxide reductase component
KPHS_248701122.268785putative voltage-gated ClC-type chloride channel
KPHS_248600111.711944dithiobiotin synthetase
KPHS_24850-1151.126563putative NAGC-like transcriptional regulator
KPHS_248401171.759795LysR family transcriptional regulator
KPHS_248302190.529862putative transport protein
KPHS_248201230.071791thermostable carboxypeptidase 1
KPHS_24810124-0.265570putative Zn-dependent carboxypeptidase
KPHS_24800428-2.100275hypothetical protein
KPHS_24790024-3.413271hypothetical protein
KPHS_24780117-2.704057hypothetical protein
KPHS_24770-210-0.475179hypothetical protein
KPHS_24760-1111.208957hypothetical protein
KPHS_24750-1111.331176multidrug efflux system protein MdtI
KPHS_24740-2121.807243multidrug efflux system protein MdtJ
KPHS_24730-2153.174276putative transport protein
KPHS_24720-1173.565542bifunctional putative acetyl-CoA:acetoacetyl-CoA
KPHS_247100164.980210triphosphoribosyl-dephospho-CoA transferase
KPHS_247000154.682020malonate decarboxylase subunit delta
KPHS_24690-1134.027917malonate decarboxylase
KPHS_24680-3122.930377MdcE
KPHS_24670-2143.033470auxin efflux carrier
KPHS_246600163.255505phosphoribosyl-dephospho-CoA transferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24830TCRTETA545e-10 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 53.7 bits (129), Expect = 5e-10
Identities = 62/295 (21%), Positives = 108/295 (36%), Gaps = 15/295 (5%)

Query: 55 VQPILPVLSNEFGVSPASSS---ISLSISTAMLAVGLLFTGPLSDAIGRKPVMVTALLLA 111
+ P+LP L + S ++ I L++ M G LSD GR+PV++ +L A
Sbjct: 24 IMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGA 83

Query: 112 ACCSLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAFSMGLYISGNSI 171
A + + I R + G++ AV Y+++ A G +
Sbjct: 84 AVDYAIMATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGF 142

Query: 172 GGMSGRLLTGVFTDFFGWRVALAAISGFALAAAIMFWRILPES--RHFRPTSLRPKTLLI 229
G ++G +L G+ F A + + +LPES RP L
Sbjct: 143 GMVAGPVLGGLMGGF-SPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLA 201

Query: 230 NFRLHWRDRGLPLLFIEGFLLM---GAFVTLFN-YIGYRLMMSPWSLSQAVVGLLSVAYL 285
+FR + L F++ L+ + R ++ ++ + L
Sbjct: 202 SFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSL 261

Query: 286 TGTWSSPKAGAMTVRFG-RGPVMLGFTAVMLCGLLLTLFSSLWLIFIGMLLFSAG 339
G + R G R +MLG A +LL + W+ F M+L ++G
Sbjct: 262 AQAMI---TGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASG 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24800IGASERPTASE300.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.4 bits (68), Expect = 0.002
Identities = 21/125 (16%), Positives = 39/125 (31%), Gaps = 9/125 (7%)

Query: 16 SSAAFAADAVSTTQAPAATHSTAAKTTHHKKHHKA--AAKPAAEQKAQAAKKHKKAEAKP 73
A A + A A + A T ++ + + + A K+ +AK
Sbjct: 1055 EQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKV 1114

Query: 74 AAAQKAQAAKKHKKVAAKPAAPQKAQAAKKHHKAAAKPAAQKAQAAKKHHKTTKHQAAKP 133
+ + K +V+ K + Q A+PA + ++
Sbjct: 1115 ETEKTQEVPKVTSQVSPKQEQSETVQPQ-------AEPARENDPTVNIKEPQSQTNTTAD 1167

Query: 134 TAQPA 138
T QPA
Sbjct: 1168 TEQPA 1172



Score = 29.6 bits (66), Expect = 0.005
Identities = 16/117 (13%), Positives = 31/117 (26%), Gaps = 7/117 (5%)

Query: 23 DAVSTTQAPAATHSTAAKTTHHKKHHKAAAKPAAEQKAQAAKKHKKAEAKPAAAQKAQAA 82
V TT + A + + A A A P+ + A
Sbjct: 990 QTVDTTNITTPNNIQADVP-------SVPSNNEEIARVDEAPVPPPAPATPSETTETVAE 1042

Query: 83 KKHKKVAAKPAAPQKAQAAKKHHKAAAKPAAQKAQAAKKHHKTTKHQAAKPTAQPAA 139
++ Q A ++ AK A +A + ++ + + Q
Sbjct: 1043 NSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTE 1099


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24770V8PROTEASE1095e-30 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 109 bits (273), Expect = 5e-30
Identities = 27/234 (11%), Positives = 70/234 (29%), Gaps = 47/234 (20%)

Query: 26 LSAKDIKTLFFGHDDRKAVNRPEESPWDAIGQLET---ASGNLCTATLISPHLALTAGHC 82
L ++ + ++DR + + + ++ + + ++ LT H
Sbjct: 61 LEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHV 120

Query: 83 LLTPPRGKPDKAVALRFI------SRKGNWVYE---IHGIDGRVDPSLGRRLKADGDGWI 133
+ AL+ N + I G D ++ + +
Sbjct: 121 V----DATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVK-FSPNEQN-- 173

Query: 134 VPSAAAPSDFGLIVLRYAPSGITPIPLFPGSKADLTAALKAADRKVTQSGYPEDH-LDNL 192
++ + P + A ++ +T +GYP D + +
Sbjct: 174 ---------------KHIGEVVKPATM-------SNNAETQVNQNITVTGYPGDKPVATM 211

Query: 193 YSHQDCIVTGWAQTSVLSHQCDTLPGDSGSPLLLKTEDGWQVIAVQSSAPGPQD 246
+ + + + + + T G+SGSP+ + +VI + +
Sbjct: 212 WESK--GKITYLKGEAMQYDLSTTGGNSGSPVF---NEKNEVIGIHWGGVPNEF 260


47KPHS_24540KPHS_24430Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_24540-1133.210185putative tartrate:succinate antiporter
KPHS_24530-1134.480970fumarase B (fumarate hydratase class I),
KPHS_245200145.426953hypothetical protein
KPHS_245100186.154142putative 4-carboxymuconolactone decarboxylase
KPHS_245000186.480107putative 3-oxoadipate enol-lactonase II
KPHS_244901196.269099putative fumarate lyase
KPHS_24480-1184.573764acetyl-CoA acetyltransferase
KPHS_24470-1174.649478acetyl-CoA:acetoacetyl-CoA transferase subunit
KPHS_24460-1154.749053acetyl-CoA:acetoacetyl-CoA transferase alpha
KPHS_24450-2133.722074IclR family transcriptional regulator
KPHS_24440-1133.114606hypothetical protein
KPHS_24430-1143.227744putative DNA-binding transcriptional regulator
48KPHS_24330KPHS_23950Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_24330215-1.915165NAD(P) transhydrogenase subunit alpha
KPHS_24320219-1.890458hypothetical protein
KPHS_24310115-0.948372hypothetical protein
KPHS_24300-115-0.265961hypothetical protein
KPHS_24290-2160.223798hypothetical protein
KPHS_242801160.755139putative arginine/ornithine antiporter
KPHS_242702161.133767short chain dehydrogenase/reductase family
KPHS_242601131.327238hypothetical protein
KPHS_242501121.231710DNA-binding transcriptional regulator RstA
KPHS_242400141.689147hypothetical protein
KPHS_242300162.199513sensor protein RstB
KPHS_24220-1162.910228DNA replication terminus site-binding protein
KPHS_24210-1162.646384fumarate hydratase
KPHS_24200-2182.838506fumarase A (fumarate hydratase class I), aerobic
KPHS_24190-2163.101564mannose-6-phosphate isomerase
KPHS_24180-2162.902929hypothetical protein
KPHS_24170-3131.933252DNA-binding transcriptional repressor MalI
KPHS_24160-2131.329559PTS system maltose and glucose-specific
KPHS_24150-1131.919736bifunctional PLP-dependent beta-cystathionase,
KPHS_241400140.768512putative Mg2+ and Co2+ transporter
KPHS_24130315-0.004109hypothetical protein
KPHS_24120316-0.238004hypothetical protein
KPHS_241101160.715668hypothetical protein
KPHS_241001140.507389paral putative membrane component of transport
KPHS_24090013-0.245801hypothetical protein
KPHS_24080014-0.307823hypothetical protein
KPHS_240700152.313549YqaE family transport protein
KPHS_240600152.771429glyceraldehyde-3-phosphate dehydrogenase
KPHS_240501193.640855aldA, aldehyde dehydrogenase A
KPHS_240401194.764728NAD-linked aldehyde dehydrogenase A
KPHS_240300194.666969hypothetical protein
KPHS_240201205.519541hypothetical protein
KPHS_240100204.907233shikimate and dehydroshikimate transport
KPHS_24000-1195.313748D-alanine/D-serine/glycine transport protein
KPHS_239900175.039942succinylglutamate desuccinylase
KPHS_23980-1133.967626putative succinylarginine dihydrolase
KPHS_23970-1143.803450succinylglutamic semialdehyde dehydrogenase
KPHS_239601111.383963arginine succinyltransferase
KPHS_23950215-0.926635bifunctional succinylornithine
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24270DHBDHDRGNASE366e-05 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 36.2 bits (83), Expect = 6e-05
Identities = 51/254 (20%), Positives = 93/254 (36%), Gaps = 28/254 (11%)

Query: 9 ILITGAGRRIGLALAHHFLQQRQPVIVSYRTPYPAIDGLREAGALCLQADFSSDDGILTF 68
ITGA + IG A+A Q + P + A A+ D +
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD--VRD 68

Query: 69 AEAVKSHTNGLRAIIHNASDWMAEKPGVPLSAVINRMMQIHVHAPYLLNHALEALLRGHG 128
+ A+ T + + D + GV +I+ + A + +N +
Sbjct: 69 SAAIDEITARIEREMGPI-DILVNVAGVLRPGLIHSLSDEEWEATFSVN-STGVFNASRS 126

Query: 129 HAASDIIHITDYVVERGSDKH-------IAYAASKAALDNMTRSFARKLAPE-VKVNAIA 180
+ + + +V GS+ AYA+SKAA T+ +LA ++ N ++
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 181 P---------SLIMFNEGDDEAYR----QQALDKSLMKIAPGEKEISDLIDYLFTSR--Y 225
P SL G ++ + L K+A +I+D + +L + + +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAK-PSDIADAVLFLVSGQAGH 245

Query: 226 VTGRSFAVDGGRPL 239
+T + VDGG L
Sbjct: 246 ITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24250HTHFIS667e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.4 bits (162), Expect = 7e-15
Identities = 26/117 (22%), Positives = 54/117 (46%), Gaps = 1/117 (0%)

Query: 3 KIVFVEDDPEVGTLIAAYLGKHDMDVVVEPRGDRAEEVIAREKPDLVLLDIMLPGKDGMT 62
I+ +DD + T++ L + DV + IA DLV+ D+++P ++
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 LCRDLRGQWQG-PIVLLTSLDSDMNHILSLEMGASDYILKTTPPAVLLARLRLHLRQ 118
L ++ P++++++ ++ M I + E GA DY+ K L+ + L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24180FLGMOTORFLIG320.007 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 31.7 bits (72), Expect = 0.007
Identities = 25/134 (18%), Positives = 52/134 (38%), Gaps = 13/134 (9%)

Query: 316 AQSQALLAKPELAQNPELYQQALTETLFNALPILLKGNPSVTISPLS-WRNAKGESTLNL 374
+Q + K + EL +++L A+ I+ ++ P R A + LN
Sbjct: 74 MMAQEFIQKGGIDYARELLEKSLGTQ--KAVDIINNLGSALQSRPFEFVRRADPANILNF 131

Query: 375 SVLLKDPAQVTAPPQTLAESLDRVVQSLDGKVV--IPVDMATEFMTKIAGLEGYQPADAA 432
++ PQT+A L + ++ +P ++ T +IA ++ P
Sbjct: 132 ---IQQEH-----PQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVR 183

Query: 433 KLADQQVKGLAAMG 446
++ K LA++
Sbjct: 184 EVERVLEKKLASLS 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24090PF01206934e-29 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 92.9 bits (231), Expect = 4e-29
Identities = 16/71 (22%), Positives = 38/71 (53%)

Query: 7 DYRLDMVGEPCPYPAVATLEAMPSLQKGEILEVVSDCPQSINNIPLDARNHGYTVLDIQQ 66
D LD G CP P + + + ++ GE+L V++ P S+ + ++ G+ +L+ ++
Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64

Query: 67 DGPTIRYLIQK 77
+ T + +++
Sbjct: 65 EDGTYHFRLKR 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_24010TCRTETB310.007 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.4 bits (71), Expect = 0.007
Identities = 37/161 (22%), Positives = 65/161 (40%), Gaps = 32/161 (19%)

Query: 59 ILSWL--SFSLTFFIRPIGGVIFAHIGDRIGRKKTLVLTLSLMGSATVAIGLLPTYEMVG 116
+W+ +F LTF IG ++ + D++G K+ L+ + + +V VG
Sbjct: 50 STNWVNTAFMLTF---SIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVI-------GFVG 99

Query: 117 LWAPALLITLRIIQGMGIGGEWGGALLAY---EYAPEKRK----GFFGSIPQAGVTIGML 169
+LLI R IQ G G AL+ Y P++ + G GSI G +G
Sbjct: 100 HSFFSLLIMARFIQ--GAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPA 157

Query: 170 MATFIVSLMTLFDEAQFLAWGWRIPFLLSSVLVFLGLWIRK 210
+ I A ++ W + L+ + + ++ K
Sbjct: 158 IGGMI---------AHYIHWSYL--LLIPMITIITVPFLMK 187


49KPHS_23790KPHS_23590Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_237900113.158301phenylacetate-CoA ligase
KPHS_237801133.897602beta-ketoadipyl CoA thiolase
KPHS_23770-1123.349111phenylacetic acid degradation protein
KPHS_237600103.6053563-hydroxybutyryl-CoA dehydrogenase
KPHS_237500143.093422enoyl-CoA hydratase
KPHS_237400141.698320enoyl-CoA hydratase-isomerase
KPHS_23730-2111.926465putative phenylacetic acid degradation NADH
KPHS_23720-2120.677749phenylacetic acid degradation protein
KPHS_23710-1111.679091phenylacetic acid degradation protein
KPHS_23700-1102.460136phenylacetic acid degradation protein PaaH
KPHS_23690-1103.373231phenylacetate-CoA oxygenase subunit PaaA
KPHS_23680-1113.971552bifunctional aldehyde dehydrogenase/enoyl-CoA
KPHS_23670-1124.219787tyramine oxidase
KPHS_236600176.425283putative oxidoreductase
KPHS_236500175.1283702-dehydropantoate 2-reductase
KPHS_23640-1174.709770phenylacetaldehyde dehydrogenase
KPHS_236302184.231001DNA-binding transcriptional activator FeaR
KPHS_236202163.820860hypothetical protein
KPHS_236100142.703943hypothetical protein
KPHS_236000132.813936hypothetical protein
KPHS_235900123.120633hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23610PF06291270.002 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 27.3 bits (60), Expect = 0.002
Identities = 9/17 (52%), Positives = 13/17 (76%)

Query: 1 MKKLLLAMAASMLLAGC 17
MKK+L + A +ML+ GC
Sbjct: 6 MKKMLFSAALAMLITGC 22


50KPHS_23430KPHS_22030Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_234302140.863955hypothetical protein
KPHS_234201150.941271aromatic amino acid transport protein
KPHS_23410017-0.576771regulator for leucine (or lrp) regulon and
KPHS_23400-1140.188186hypothetical protein
KPHS_23390-1151.763346aromatic amino acid aminotransferase
KPHS_23380-1151.804898ABC transporter permease
KPHS_233700152.644793putative ABC transport system ATP-binding
KPHS_233600163.920551putative lipoprotein
KPHS_23350-1185.545044putative aminotransferase
KPHS_23340-1196.274210zinc-binding oxidoreductase
KPHS_233301205.838290putative TetR family transcriptional regulator
KPHS_23320-1185.190075hypothetical protein
KPHS_233101174.102774hypothetical protein
KPHS_233000174.963294transcriptional regulator LysR
KPHS_23290-1175.477079transcriptional regulator LysR
KPHS_232801165.575604LysR family transcriptional regulator
KPHS_232701165.582489putative 2'-hydroxyisoflavone reductase
KPHS_232600165.289985hypothetical protein
KPHS_23250-1155.398202putative ARAC-type regulatory protein
KPHS_23240-1153.917097putative dehydrogenase/reductase oxidoreductase
KPHS_23230-1163.540350putative regulatory protein TetR
KPHS_23220-2142.124956oxidoreductase
KPHS_23210-1141.032303hypothetical protein
KPHS_23200-1130.872243hypothetical protein
KPHS_231901111.627818hypothetical protein
KPHS_231801122.066322hypothetical protein
KPHS_231702121.654945hypothetical protein
KPHS_231602161.666868type VI secretion-associated protein
KPHS_231502151.715732type VI secretion-associated protein
KPHS_231402151.673735ImcF domain-containing protein
KPHS_231303190.920412hypothetical protein
KPHS_23120321-0.258048PaaR repeat-containing protein
KPHS_23110422-0.266216hypothetical protein
KPHS_23100422-1.512100hypothetical protein
KPHS_230903210.411172putative lipoprotein
KPHS_230801132.410328putative lipoprotein
KPHS_230702122.000041putative lipoprotein
KPHS_230603123.077406putative lipoprotein
KPHS_230503123.317574hypothetical protein
KPHS_230404124.015323Rhs element Vgr protein
KPHS_230303113.717094putative protease
KPHS_23020181.749578hypothetical protein
KPHS_23010-181.780292OmpA/MotB domain-containing protein
KPHS_23000-110-0.586832hypothetical protein
KPHS_22990015-1.086927hypothetical protein
KPHS_22980119-3.264315EvpB/family type VI secretion protein
KPHS_22970229-6.484969hypothetical protein
KPHS_22960329-6.800242hypothetical protein
KPHS_22950329-6.284584DinI family protein
KPHS_22940329-5.920910putative reverse transcriptase/maturase
KPHS_22930433-8.064757hypothetical protein
KPHS_22920534-7.249724hypothetical protein
KPHS_22910841-7.436144hypothetical protein
KPHS_22900641-5.495386hypothetical protein
KPHS_22890641-7.217268hypothetical protein
KPHS_22880541-8.032200putative bacteriophage protein
KPHS_22870341-8.406219putative bacteriophage protein
KPHS_22860338-9.423575putative bacteriophage protein
KPHS_22850339-9.567105putative bacteriophage protein
KPHS_22840440-11.030996hypothetical protein
KPHS_22830130-6.429242hypothetical protein
KPHS_22820129-5.871429hypothetical protein
KPHS_22810227-5.007962putative bacteriophage protein
KPHS_22800126-3.995990putative bacteriophage protein
KPHS_22790124-3.768450hypothetical protein
KPHS_22780021-3.048725lytic transglycosylase, catalytic
KPHS_22770321-2.988574hypothetical protein
KPHS_22760222-2.988036hypothetical protein
KPHS_22750323-3.426734Putative bacteriophage protein
KPHS_22740123-3.622201putative bacteriophage protein
KPHS_22730423-3.446893putative bacteriophage protein
KPHS_22720126-3.506260hypothetical protein
KPHS_22710129-3.457973putative bacteriophage protein
KPHS_22700128-3.574831putative bacteriophage protein
KPHS_22690026-4.165567hypothetical protein
KPHS_22680026-4.178433putative bacteriophage protein
KPHS_22670-128-4.452277Putative bacteriophage protein
KPHS_22660-127-4.782687hypothetical protein
KPHS_22650027-5.398407phage-associated protein, family
KPHS_22640029-5.687069putative bacteriophage protein
KPHS_22630231-2.825525putative bacteriophage protein
KPHS_22620230-2.736211hypothetical protein
KPHS_22610230-2.803032hypothetical protein
KPHS_22600229-2.646142hypothetical protein
KPHS_22590324-2.476185hypothetical protein
KPHS_22580324-4.162394hypothetical protein
KPHS_22570330-7.331400hypothetical protein
KPHS_22560327-6.808203hypothetical protein
KPHS_22550226-6.909059hypothetical protein
KPHS_22540129-8.206438putative antitermination protein
KPHS_22530032-9.020794hypothetical protein
KPHS_22520031-8.633197putative phage DNA primase
KPHS_22510131-9.625029putative helicase
KPHS_22500239-11.633920hypothetical protein
KPHS_22490142-11.611920hypothetical protein
KPHS_22480331-7.099136hypothetical protein
KPHS_22470227-6.702438hypothetical protein
KPHS_22460327-6.634625hypothetical protein
KPHS_22450327-6.842563hypothetical protein
KPHS_22440327-7.591530hypothetical protein
KPHS_22430331-7.131867gp20
KPHS_22420234-7.351146hypothetical protein
KPHS_22410238-8.460981DNA-binding protein Roi
KPHS_22400036-5.977404hypothetical protein
KPHS_22390036-10.241740hypothetical protein
KPHS_22380-132-8.207616hypothetical protein
KPHS_22370-127-5.928344hypothetical protein
KPHS_22360027-5.636291hypothetical protein
KPHS_22350022-4.151608putative intracellular protease/amidase
KPHS_22340024-5.339784putative metal-dependent phosphohydrolase with
KPHS_22330-1200.639356short chain dehydrogenase/reductase family
KPHS_22320020-0.662183LysR family transcriptional regulator
KPHS_22310122-2.156628hypothetical protein
KPHS_22300229-5.584055glycosyl hydrolase, family 2
KPHS_22290229-5.943205putative aldo/keto reductase family
KPHS_22280321-4.435605putative MFS-family transport protein
KPHS_22270421-6.191967hypothetical protein
KPHS_22260317-4.631438putative LysR-family transcriptional regulator
KPHS_22250114-2.616779hypothetical protein
KPHS_22240-1111.757319hypothetical protein
KPHS_22230-1133.129644periplasmic murein tripeptide
KPHS_222202154.274879hypothetical protein
KPHS_222102133.898838murein peptide amidase A
KPHS_222002123.200136L-Ala-D/L-Glu epimerase, a muconate lactonizing
KPHS_221901133.145018thiol peroxidase
KPHS_221801153.253163putative transport protein
KPHS_221701143.088243DNA-binding transcriptional regulator TyrR
KPHS_221600152.733930putative alpha-galactosidase/6-phospho-beta-
KPHS_221501193.299311putative LysR family regulatory protein
KPHS_221404192.872015hypothetical protein
KPHS_221304152.076464hypothetical protein
KPHS_221204162.585999hypothetical protein
KPHS_221102162.810831DNA-binding transcriptional activator PspC
KPHS_221003174.199840phage shock protein B
KPHS_220902164.569737phage shock protein PspA
KPHS_220802175.389429phage shock protein operon transcriptional
KPHS_220701155.230189major facilitator family transporter
KPHS_220601155.087196putative amino acid amidohydrolase
KPHS_220500144.654474putative helix-turn-helix AraC-type
KPHS_22040-1154.131315hypothetical protein
KPHS_220300163.384495hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23330HTHTETR593e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.9 bits (142), Expect = 3e-13
Identities = 28/166 (16%), Positives = 58/166 (34%), Gaps = 12/166 (7%)

Query: 1 MRADARKNYDLLIEVARDVFVEQGAEA-SLRDIARRAGVGMGTLYRHFPNRDSLLEAVLR 59
+ +A++ +++VA +F +QG + SL +IA+ AGV G +Y HF ++ L +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 60 SRFAALTARAESLL------LAADPAAALLEWLAESVAFTHQHRGIIAPLMSAIDDPESA 113
+ + + L+ L +V + + E A
Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMA 124

Query: 114 L-----HSACVALRAAGTSLLTRAQQAGLARPDLSGEELFDLIAAL 154
+ + C+ L +A + DL ++
Sbjct: 125 VVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGY 170


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23320NUCEPIMERASE406e-06 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 40.2 bits (94), Expect = 6e-06
Identities = 29/129 (22%), Positives = 47/129 (36%), Gaps = 19/129 (14%)

Query: 6 TVLVFGATGQQGGSVARALLHRGWRVRALVRDPFSAG---------AAALAARGAELVVG 56
LV GA G G V++ LL G +V + D + LA G +
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGI--DNLNDYYDVSLKQARLELLAQPGFQFHKI 59

Query: 57 TFEDRAAMRSAMA--GVDGVF------SVQPSSPGGTVTDEQEVRYGITIADLAVECGVK 108
DR M A + VF +V+ S + + + I + ++
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 109 HLVYSSGSA 117
HL+Y+S S+
Sbjct: 120 HLLYASSSS 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23300PF05043344e-04 Transcriptional activator
		>PF05043#Transcriptional activator

Length = 493

Score = 33.8 bits (77), Expect = 4e-04
Identities = 17/66 (25%), Positives = 29/66 (43%), Gaps = 1/66 (1%)

Query: 1 MNKIIENDFSRIDLNLLTVLMVLYREGSVTRTAEVLHLGQPAISGALKRLREMFDDPLFV 60
M ++ R L LL +L R + AE+L+ + A+ L ++ F D +F
Sbjct: 1 MRDLLSKKSHR-QLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFH 59

Query: 61 RSARGM 66
S G+
Sbjct: 60 SSTNGI 65


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23240DHBDHDRGNASE1036e-29 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 103 bits (259), Expect = 6e-29
Identities = 67/258 (25%), Positives = 110/258 (42%), Gaps = 16/258 (6%)

Query: 3 KIALITGANRGLGRQTALDIARQGGDVIVTYRGSLEQAEAVVADIRALGRKAIALPLDMA 62
KIA ITGA +G+G A +A QG + + E+ E VV+ ++A R A A P D+
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHI-AAVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 63 QTASFPAFADSLGSALASVWGRATFDHLINNAGHGEFAPLAETREAQFDGLFNVHVKGVF 122
+A+ + + D L+N AG + + +++ F+V+ GVF
Sbjct: 68 DSAAIDEITARIEREMGP------IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVF 121

Query: 123 FLVQTLLPLLAD--GGRIVNFSSGLTRVSYPGFSAYAAAKAAVEMLSVYMARELGGRGIT 180
+++ + D G IV S V +AYA++KAA M + + EL I
Sbjct: 122 NASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181

Query: 181 VNTIAPGAIATDFGGGL-VRDDAEVN------AQFAAMTALGRVGVPEDIGPMIASLLRD 233
N ++PG+ TD L ++ F L ++ P DI + L+
Sbjct: 182 CNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 234 DNRWVTAQRIEVSGGQTI 251
+T + V GG T+
Sbjct: 242 QAGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23230HTHTETR456e-08 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 44.6 bits (105), Expect = 6e-08
Identities = 19/123 (15%), Positives = 47/123 (38%), Gaps = 2/123 (1%)

Query: 6 PGRTPGRPRQFDAEQAIETAQRLFHARGYDAVSVADLTHAFGINPPSFYAAFGNKLGLYT 65
+T ++ + ++ A RLF +G + S+ ++ A G+ + Y F +K L++
Sbjct: 2 ARKTKQEAQE-TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 66 RVLQR-YSQTGAIPIDALLRDDQPVAASLIAVLQEAARRYVADPAAAGCLVLEGVHCQDA 124
+ + S G + ++ + + L +L V + + + C+
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 125 DAR 127

Sbjct: 121 GEM 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23220DHBDHDRGNASE812e-20 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 80.9 bits (199), Expect = 2e-20
Identities = 65/249 (26%), Positives = 111/249 (44%), Gaps = 24/249 (9%)

Query: 7 KSVLVLGGSRGIGAAIVRRFVADGASVVFSYSGSPEAAERLAAETGSTA-----VQADSA 61
K + G ++GIG A+ R + GA + + +PE E++ + + A AD
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 62 DRDAVISLV----RDSGPLDVLVVNAGIALFGDALEQDSDAIDRLFRINIHAPYHASVEA 117
D A+ + R+ GP+D+LV AG+ G + + F +N ++AS
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 118 ARRMP--EGGRIIVIGSVNGDRMPLPGMAAYALSKSALQGLARGLARDFGPRGITVNVVQ 175
++ M G I+ +GS N +P MAAYA SK+A + L + I N+V
Sbjct: 128 SKYMMDRRSGSIVTVGS-NPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 176 PGPIDTDA--------NPGNGPMKELMHSF---MAIKRHGRPEEVAGMVAWLAGPEASFV 224
PG +TD N +K + +F + +K+ +P ++A V +L +A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 225 TGAMHTIDG 233
T +DG
Sbjct: 247 TMHNLCVDG 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23030HTHFIS320.015 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.7 bits (72), Expect = 0.015
Identities = 30/105 (28%), Positives = 42/105 (40%), Gaps = 17/105 (16%)

Query: 580 GKRVVGQEAALSAIARRL-RAAKTGLTPENGPQGVFLLVGPSGTGKTETALALADALFGG 638
G +VG+ AA+ I R L R +T LT ++ G SGTGK A AL D
Sbjct: 136 GMPLVGRSAAMQEIYRVLARLMQTDLT--------LMITGESGTGKELVARALHDYGKRR 187

Query: 639 EKALITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAVRKRP 683
+ IN++ S+L G + G T A +
Sbjct: 188 NGPFVAINMAAIPRDLIESELFGH--------EKGAFTGAQTRST 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_23010OMPADOMAIN931e-22 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 92.7 bits (230), Expect = 1e-22
Identities = 45/146 (30%), Positives = 63/146 (43%), Gaps = 12/146 (8%)

Query: 416 PPPPPRPVQRVAPDVIRLDSMSLFDTGKWVLKPGSTKRL--VSSLMDIKARPGWLIVVAG 473
P P P V L S LF+ K LKP L + S + +VV G
Sbjct: 200 VAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLG 259

Query: 474 HTDSVGEEKANQLLSLKRAESVRDWMRDTGDVPDSCFAVQGYGESRPIATNDT------- 526
+TD +G + NQ LS +RA+SV D++ G +P + +G GES P+ N
Sbjct: 260 YTDRIGSDAYNQGLSERRAQSVVDYLISKG-IPADKISARGMGESNPVTGNTCDNVKQRA 318

Query: 527 --PEGRALNRRVEISLVPQVDACRLP 550
+ A +RRVEI + D P
Sbjct: 319 ALIDCLAPDRRVEIEVKGIKDVVTQP 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22330DHBDHDRGNASE732e-17 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 73.2 bits (179), Expect = 2e-17
Identities = 64/254 (25%), Positives = 102/254 (40%), Gaps = 15/254 (5%)

Query: 6 KIALVTGGSRGLGRATVEALAQRGVNVVLTYKTRLAEANEVVTRVEALGARAIALPFSAG 65
KIA +TG ++G+G A LA +G ++ + +VV+ ++A A A P
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHI-AAVDYNPEKLEKVVSSLKAEARHAEAFP---- 63

Query: 66 EIDTFDAFVSAFQGALTELGADKFDYLVNNAGNASGMGFLNATEAEFDALYCIHVKSVFF 125
D D+ A E D LVN AG + ++ E++A + ++ VF
Sbjct: 64 -ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122

Query: 126 LSQKLLPLLAD--GGRIVNVSSGLTRIVMANRAPYAIMKSAVETLTRYMAFELGSRGITV 183
S+ + + D G IV V S + + A YA K+A T+ + EL I
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 184 NCVAPGAIATDFSGGVVRDNPQVAQAVANMTA-------LGRPGLPEDIGPMIASLLSDD 236
N V+PG+ TD + D Q + L + P DI + L+S
Sbjct: 183 NIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 237 HRWVNAQRIEVSGG 250
+ + V GG
Sbjct: 243 AGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22320SURFACELAYER310.005 Lactobacillus surface layer protein signature.
		>SURFACELAYER#Lactobacillus surface layer protein signature.

Length = 439

Score = 31.2 bits (70), Expect = 0.005
Identities = 22/90 (24%), Positives = 40/90 (44%), Gaps = 10/90 (11%)

Query: 11 VRIVERGSFSAAAADLGVSRPVATAAIKALEVSLGARLLHRTTRHVRPTAEGSLYYQRCV 70
+RIV SAAAA L P+A A+ V+ + + + A+ + +
Sbjct: 5 LRIV-----SAAAAALLAVAPIAAT---AMPVNAATTINADSAINANTNAKYDVDVTPSI 56

Query: 71 SILAALEEANRSAG--GSISGTIRVDVAGN 98
S +AA+ +++ GS++G+I G
Sbjct: 57 SAIAAVAKSDTMPAIPGSLTGSISASYNGK 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22280TCRTETB330.002 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 33.3 bits (76), Expect = 0.002
Identities = 21/113 (18%), Positives = 45/113 (39%), Gaps = 2/113 (1%)

Query: 76 RKWLLLGLTALMAASGVIIALASSFPVYMLGRALIGIVIGGFWSMSAATAIRLVPQRQVP 135
++ LL G+ S + S F + ++ R + G F ++ R +P+
Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138

Query: 136 RALAIFNGGNALATVVAAPLGSYLGATVGWRGAFLYLVPLAVLAFVWQCISLP 188
+A + A+ V +G + + W ++L L+P+ + V + L
Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYIHW--SYLLLIPMITIITVPFLMKLL 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22170HTHFIS308e-101 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 308 bits (790), Expect = e-101
Identities = 112/377 (29%), Positives = 172/377 (45%), Gaps = 38/377 (10%)

Query: 174 VLTGAVAMLRSTVRMGRQLQTMTSQDTSAFSQILAVGPKMRHVVEQARKLAMLSAPLLIV 233
LT + ++ + ++ + D+ ++ M+ + +L L+I
Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMIT 166

Query: 234 GDTGTGKDLLAHACHLASPRAGKPYLALNCGSIPEDAVESELFG-------DALQGKKGF 286
G++GTGK+L+A A H R P++A+N +IP D +ESELFG A G
Sbjct: 167 GESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGR 226

Query: 287 FEQANGGSVLLDEIGEMSPRMQTKLLRFLNDGTFRRVGEDHEVHVDVRVICATQKNLIEL 346
FEQA GG++ LDEIG+M QT+LLR L G + VG + DVR++ AT K+L +
Sbjct: 227 FEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQS 286

Query: 347 VQKGLFREDLYYRLNVLTLYLPPLRDCPQDIMPLTELFVARFADEQGIPRPKLSADLSTV 406
+ +GLFREDLYYRLNV+ L LPPLRD +DI L FV + E G+ + + +
Sbjct: 287 INQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALEL 345

Query: 407 LTRYSWPGNVRQLKNAVYRALTQLEGFELRPQDILLP---------------DHDVASLP 451
+ + WPGNVR+L+N V R + + I S+
Sbjct: 346 MKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSIS 405

Query: 452 VGEEAM--------------EGSLDDITRRFERSVLTQ-LYRSYPSTRKLAKRLGVSHTA 496
E G D + E ++ L + + K A LG++
Sbjct: 406 QAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNT 465

Query: 497 IANKLREYGLSQKKGDE 513
+ K+RE G+S +
Sbjct: 466 LRKKIRELGVSVYRSSR 482


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22080HTHFIS344e-118 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 344 bits (884), Expect = e-118
Identities = 127/346 (36%), Positives = 183/346 (52%), Gaps = 26/346 (7%)

Query: 7 AQYKDNLLGEANSFLEVLEQVSRLAPLDKPVLVIGERGTGKELIANRLHYLSSRWQGPFI 66
+Q L+G + + E+ ++RL D +++ GE GTGKEL+A LH R GPF+
Sbjct: 133 SQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFV 192

Query: 67 SLNCAALNDNLLDSELFGHEAGAFTGASKRHPGRFERADGGTLFLDELATAPMLVQEKLL 126
++N AA+ +L++SELFGHE GAFTGA R GRFE+A+GGTLFLDE+ PM Q +LL
Sbjct: 193 AINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLL 252

Query: 127 RVIEYGELERVGGSQPLQVNVRLVCATNADLPQMVEEGHFRADLLDRLAFDVVQLPPLRD 186
RV++ GE VGG P++ +VR+V ATN DL Q + +G FR DL RL ++LPPLRD
Sbjct: 253 RVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRD 312

Query: 187 RQSDIMLLANQFAIQMCRELGLPLFPGFSERATATLLGYRWPGNIRELKNVVERSVYRHG 246
R DI L F Q +E F + A + + WPGN+REL+N+V R +
Sbjct: 313 RAEDIPDLVRHFVQQAEKEGLDVK--RFDQEALELMKAHPWPGNVRELENLVRRLTALYP 370

Query: 247 DSE--------HELDAIIINPFRQSPG---------------SPPEAAPGDELPALPLDL 283
I +P ++ A+ GD LP L
Sbjct: 371 QDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGL-Y 429

Query: 284 RDFQLQQEKRLLQRSLEQAKYHQKQAAELLGLTYHQLRALLKKHQL 329
+ E L+ +L + +Q +AA+LLGL + LR +++ +
Sbjct: 430 DRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGV 475


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22070TCRTETB894e-21 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 88.8 bits (220), Expect = 4e-21
Identities = 81/415 (19%), Positives = 161/415 (38%), Gaps = 34/415 (8%)

Query: 28 FWLFAQSVINVVPAMISSLDISLETLTLAVSLS-----------ALFSGCFVVASGGLAD 76
WL S +V+ M+ L++SL + + L G L+D
Sbjct: 17 IWLCILSFFSVLNEMV--LNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD 74

Query: 77 KFGRMRMTTIGLGLSIVGSAMLVVAQGP-GLFLAGRVLQGLSAACIMPATLALIKTWYEG 135
+ G R+ G+ ++ GS + V L + R +QG AA + ++ +
Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK 134

Query: 136 RARQRAVSFWVIGSWGGSGLCSFVGGAIATGLGWRWIFVFSIAVALLALFLLRGTPESRS 195
R +A G G+ +GG IA + W ++ + + + FL++ +
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKK--E 192

Query: 196 ASASQHKLDVGGLLSLIVALVLVNLFISKGHGWGWSSPLSLTMLAGALAAGTIFIRNGMR 255
H D+ G++ + V +V LF + + + L+ L IF+++ +R
Sbjct: 193 VRIKGH-FDIKGIILMSVGIVFFMLF-TTSYSISFLIVSVLSFL--------IFVKH-IR 241

Query: 256 KGEAALIDFALFRNRAYGAAVLSNFLLNGAI-GTMMIASIWLQQGHHLTPLESGMMTLGY 314
K +D L +N + VL ++ G + G + + ++ H L+ E G + + +
Sbjct: 242 KVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVII-F 300

Query: 315 LVTVLAMIR--VGEKLLQRYGARLPMMAGPVLTAIAIALISCTFLEKALYIGVVFASNVL 372
T+ +I +G L+ R G + G +++ L + LE + + +
Sbjct: 301 PGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSF-LTASFLLETTSWF-MTIIIVFV 358

Query: 373 FGLGLGCYATPSTDTAVANAPENKIGVASGIYKMGSSLGGAMGIAVTASLFALFL 427
G GL T + ++ + + G + S L GIA+ L ++ L
Sbjct: 359 LG-GLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPL 412


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_22030NUCEPIMERASE473e-08 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 47.5 bits (113), Expect = 3e-08
Identities = 21/83 (25%), Positives = 39/83 (46%), Gaps = 11/83 (13%)

Query: 2 MRIFLTGASGFIGSRILPALQASGHKVIGL---------ARSESTAQALKAAGAEVHRGT 52
M+ +TGA+GFIG + L +GH+V+G+ + ++ + L G + H+
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 53 LDAPESL--LAGVGNADAVIHTA 73
L E + L G+ + V +
Sbjct: 61 LADREGMTDLFASGHFERVFISP 83


51KPHS_21780KPHS_21630Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_21780214-3.778828hypothetical protein
KPHS_21770013-2.510748hypothetical protein
KPHS_21760113-2.445404transcriptional regulator CysB
KPHS_21750212-1.512696DNA topoisomerase I
KPHS_21740013-1.177480hypothetical protein
KPHS_21730012-0.036151hypothetical protein
KPHS_21720-1101.452446Peptidase S49 domain protein
KPHS_21710-1102.098682hypothetical protein
KPHS_21700-2102.097104short chain dehydrogenase
KPHS_21690-3122.390836cob(I)yrinic acid a,c-diamide
KPHS_21680-392.78768223S rRNA pseudouridylate synthase B
KPHS_21670-1103.351299putative LysR family regulatory protein
KPHS_21660-2123.114488putative metabolite transporter
KPHS_21650-2163.128648hypothetical protein
KPHS_21640-1183.795723putative enzyme of the trp operon
KPHS_21630-2133.343159anthranilate synthase component I
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_21700DHBDHDRGNASE943e-25 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 94.3 bits (234), Expect = 3e-25
Identities = 62/239 (25%), Positives = 102/239 (42%), Gaps = 22/239 (9%)

Query: 13 RIILVTGASDGIGREAALTYARYSASVILLGRNDDKLRTVAQEIEREGGIPPRWFTLDLL 72
+I +TGA+ GIG A T A A + + N +KL V ++ E F D+
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEA-FPADV- 66

Query: 73 TCTPQACQQLAQQISMHYPRLDGVLHNAGLLGDICPMEEQKPEVWQQVMQVNVNGTFMLT 132
A ++ +I +D +++ AG+L + E W+ VN G F +
Sbjct: 67 -RDSAAIDEITARIEREMGPIDILVNVAGVL-RPGLIHSLSDEEWEATFSVNSTGVFNAS 124

Query: 133 QALLPLLLRSESGSLVFTSSSVGRQGRANWGAYAVSKFATEGMMQVLADEYQSRHLRVNC 192
+++ ++ SGS+V S+ R + AYA SK A + L E ++R N
Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 193 INPGGTRTGMRASAFPTED-----------------PL-KLKTPADIMPVYLWLMGDDS 233
++PG T T M+ S + E+ PL KL P+DI L+L+ +
Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243


52KPHS_21350KPHS_21090Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_21350-211-3.256507hypothetical protein
KPHS_21340-113-4.8866206-phospho-beta-glucosidase
KPHS_21330118-6.276126DNA-binding transcriptional regulator ChbR
KPHS_21320019-4.421620PTS system N,N'-diacetylchitobiose-specific
KPHS_21310118-4.328339PEP-dependent phosphotransferase enzyme II
KPHS_21300119-2.563833PEP-dependent phosphotransferase enzyme II
KPHS_21290-117-1.983451hypothetical protein
KPHS_21280017-1.906289PTS system, N,N'-diacetylchitobiose-specific IIB
KPHS_212700141.042003DNA-binding transcriptional activator OsmE
KPHS_212600162.925245NAD synthetase
KPHS_21250-1185.301540nucleotide excision repair endonuclease
KPHS_212400174.983121hypothetical protein
KPHS_21230-1175.605106hypothetical protein
KPHS_21220-1155.134292succinylglutamate desuccinylase
KPHS_21210-1154.835141succinylarginine dihydrolase
KPHS_21200-1174.439631aldehyde dehydrogenase
KPHS_211900203.557852arginine N-succinyltransferase
KPHS_21180-1193.701879bifunctional
KPHS_211700184.939130exodeoxyribonuclease III
KPHS_211604178.577421hypothetical protein
KPHS_211503168.877860hypothetical protein
KPHS_211404168.624786hypothetical protein
KPHS_211304168.462139hypothetical protein
KPHS_211204178.614251hypothetical protein
KPHS_211105167.947058putative ABC transporter permease
KPHS_211000154.817095ATPase
KPHS_21090-1143.861588putative thiosulfate sulfur transferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_21100PF05272280.029 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.1 bits (62), Expect = 0.029
Identities = 15/44 (34%), Positives = 21/44 (47%), Gaps = 4/44 (9%)

Query: 11 VRRQPLLQEVAFSVAPG----EVLTLMGPSGSGKSTLFAWMIGA 50
V + L+ VA + PG + L G G GKSTL ++G
Sbjct: 576 VGKYILMGHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLVGL 619


53KPHS_20960KPHS_20490Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_20960-112-3.359700signal peptide peptidase SppA
KPHS_20950122-5.884598L-asparaginase I
KPHS_20940327-7.412361nicotinamidase/pyrazinamidase
KPHS_20930430-8.540464metabolite transport protein
KPHS_20920431-8.599183hypothetical protein
KPHS_20910328-7.494903putative oxidoreductase
KPHS_20900529-7.869547putative sugar kinase
KPHS_20890422-5.855419putative aldolase
KPHS_20880322-5.347370putative oxidoreductase, Zn-dependent and
KPHS_20870120-4.982626metabolite transporter YdjK
KPHS_20860115-3.401838putative oxidoreductase, Zn-dependent and
KPHS_20850224-3.500747zinc-binding dehydrogenase family
KPHS_20840222-2.438613putative chitinase II
KPHS_20830121-1.926370hypothetical protein
KPHS_20820120-1.828749hypothetical protein
KPHS_20810118-2.600054methionine sulfoxide reductase B
KPHS_20800117-2.667416glyceraldehyde-3-phosphate dehydrogenase
KPHS_20790-110-2.334048hypothetical protein
KPHS_20780011-2.414292putative an aldehyde reductase
KPHS_20770111-3.121460scaffolding protein for murein-synthesising
KPHS_20760013-3.247927hypothetical protein
KPHS_20750015-0.169548hypothetical protein
KPHS_207400163.199717hypothetical protein
KPHS_20730-1135.297746putative glutathione peroxidase
KPHS_20720-1155.526306hypothetical protein
KPHS_207100165.019078hypothetical protein
KPHS_207000174.836116putative LysR-family transcriptional regulator
KPHS_20690-3183.188893sugar efflux transporter
KPHS_20680-2211.981824oxidoreductase
KPHS_20670-2190.029807putative LysR-family transcriptional regulator
KPHS_20660-321-1.329588DNA polymerase V subunit UmuC
KPHS_20650-221-1.801765DNA polymerase V subunit UmuD
KPHS_20640-121-1.483147putative reverse transcriptase/maturase
KPHS_20630-119-0.231245putative DinI-like protein
KPHS_206201202.715331putative glycosyl transferase
KPHS_206102195.406081hypothetical protein
KPHS_206002186.650646hypothetical protein
KPHS_205903196.695086hypothetical protein
KPHS_205804187.127528putative ARAC-type regulatory protein
KPHS_205701165.616551putative amino acid/amine transport protein
KPHS_20560-2193.500409CTP synthase domain protein
KPHS_20550-2181.949819hypothetical protein
KPHS_20540-219-0.170208hypothetical protein
KPHS_20530-118-1.425432hypothetical protein
KPHS_20520-117-1.947150hypothetical protein
KPHS_20510319-4.209605hypothetical protein
KPHS_20500420-4.984548hypothetical protein
KPHS_20490519-3.183866hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20930TCRTETB394e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 38.7 bits (90), Expect = 4e-05
Identities = 25/118 (21%), Positives = 48/118 (40%), Gaps = 1/118 (0%)

Query: 65 ALMFGYFIGSLTGGFIGDYLGRRKAFRLNLLIVGISACVATFVPNMY-WLIFCRCLMGCG 123
A M + IG+ G + D LG ++ ++I + + + + LI R + G G
Sbjct: 57 AFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAG 116

Query: 124 MGALIMIGYASFTEFIPPAVRGKWSARLSFVGNWSPMLSAAIGIVVIAFLSWRIMFLL 181
A + +IP RGK + + + AIG ++ ++ W + L+
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLI 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20870TCRTETB310.007 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.4 bits (71), Expect = 0.007
Identities = 26/102 (25%), Positives = 43/102 (42%), Gaps = 4/102 (3%)

Query: 25 LKELGWTDNSTTATFSAITTAGMFLGALGG---GIIGDKIGRKNAFILYEAIHIIAMVVG 81
L ++ N A+ + + TA M ++G G + D++G K + I+ V+G
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 82 AFSPNMTF-LIACRFVMGVGLGALLVTLFAGFTEYMPGRNRG 122
+ LI RF+ G G A + Y+P NRG
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20690TCRTETB479e-08 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 46.8 bits (111), Expect = 9e-08
Identities = 34/142 (23%), Positives = 61/142 (42%), Gaps = 1/142 (0%)

Query: 40 LSALAADFHQTESGVGLAVTAYGWVGALAALLSGAMPARISRKALLVGLMLILAFSCLAA 99
L +A DF++ + TA+ ++ + G + ++ K LL+ ++I F +
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 100 TRSYSMFA-LMSARMIGALAHGAFWALIGIVAAQLVPPHRLGLATAIIFGGVSAASVVGV 158
+S F+ L+ AR I AF AL+ +V A+ +P G A +I V+ VG
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 159 PLASFIATLAGWRLAFMSMALL 180
+ IA W + +
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMIT 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20680DHBDHDRGNASE1228e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 122 bits (306), Expect = 8e-36
Identities = 70/254 (27%), Positives = 120/254 (47%), Gaps = 10/254 (3%)

Query: 2 SKKLADKVALVTGGSAGIGLASAKALAEQGAKVY---ITGRRQEELDAAVRFIGPAARGI 58
+K + K+A +TG + GIG A A+ LA QGA + + E++ ++++ A
Sbjct: 3 AKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF 62

Query: 59 RADAAVLSDLDAVFATIAEESGRLDVLFANAGGGDMLPLSAITEAHVDRIFATNVRGVVF 118
AD + +D + A I E G +D+L AG + ++++ + F+ N GV
Sbjct: 63 PADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122

Query: 119 TVQKALPLLAD--GASVILTGSTAAVKGTANFSIYSASKAAVRSLARSWALEVSDRGIRI 176
+ + D S++ GS A + + Y++SKAA + LE+++ IR
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 177 NVVSPGPVRTPGLGGLVAEADRQ-----GLFDALAAGVPLGRLGEPEEIGRTVVFLASDE 231
N+VSPG T L A+ + G + G+PL +L +P +I V+FL S +
Sbjct: 183 NIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 232 SSFINAAEIYVDGG 245
+ I + VDGG
Sbjct: 243 AGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20590PRTACTNFAMLY270.045 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 26.6 bits (58), Expect = 0.045
Identities = 17/64 (26%), Positives = 26/64 (40%)

Query: 46 VEKQGLTVGIIILTIGVMAPIASGTLPPSTLIHSFMNWKSLLAIAVGVFVSWLGGRGVSL 105
V Q + L IG + + LPPS ++ N ++ A VS LG ++L
Sbjct: 171 VTVQRSAIVDGGLHIGALQSLQPEDLPPSRVVLRDTNVTAVPASGAPAAVSVLGASELTL 230

Query: 106 MGSQ 109
G
Sbjct: 231 DGGH 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20530BORPETOXINB250.026 Bordetella pertussis toxin B subunit signature.
		>BORPETOXINB#Bordetella pertussis toxin B subunit signature.

Length = 226

Score = 25.4 bits (55), Expect = 0.026
Identities = 13/38 (34%), Positives = 17/38 (44%), Gaps = 3/38 (7%)

Query: 4 LLAILPLALAGCTQPQPTAPTKTIGMPNPAAVYCQQSG 41
LL++LPLAL G + + P I P Q G
Sbjct: 11 LLSVLPLALLGSHVARASTPGIVI---PPQEQITQHGG 45


54KPHS_20400KPHS_20290Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_20400219-1.100575putative carboxylyase-related protein
KPHS_20390119-2.161381hypothetical protein
KPHS_20380016-1.522251hypothetical protein
KPHS_20370-2120.113848hypothetical protein
KPHS_20360-211-0.678402hypothetical protein
KPHS_20350-113-2.670309hypothetical protein
KPHS_20340-112-2.482134cyanide-insensitive oxidase CioB
KPHS_20330-117-4.059684cytochrome oxidase bd-II subunit I
KPHS_20320121-4.066589hypothetical protein
KPHS_20310224-4.574087hypothetical protein
KPHS_20300426-4.002382putative structural protein
KPHS_20290225-1.773160hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20340PF06580300.011 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.2 bits (68), Expect = 0.011
Identities = 26/137 (18%), Positives = 43/137 (31%), Gaps = 12/137 (8%)

Query: 110 SHRIFWDYAFAGGSLLATFSQGIVVGAFINGFAVADRRFAGSTLDWLTPFNLFCGLGLVV 169
+++ +W G + G A S + GL L
Sbjct: 8 ANKYYWYCQGIGWGVYTLTGFGFASLYGSPKLHSMIFNIAISLM----------GLVLTH 57

Query: 170 AYLLLGTTWLIMKSEGALQQRMRELTRKVLLALMAVIAVVSVWTPLGWRYVAERWFTLPN 229
AY +K Q +R L V++ ++ +A S+W L + FTLP
Sbjct: 58 AYRSFIKRQGWLKL-NMGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPL 116

Query: 230 FF-WFVPVPILVLALSL 245
V ++ SL
Sbjct: 117 ALSIIFNVVVVTFMWSL 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_20290PF03309270.003 Bvg accessory factor
		>PF03309#Bvg accessory factor

Length = 271

Score = 27.4 bits (61), Expect = 0.003
Identities = 13/61 (21%), Positives = 22/61 (36%), Gaps = 5/61 (8%)

Query: 2 VVPVI---LLKTVKKLLKQVVKVVSTAAGTLKM-IRSVRPKRVKKADRIATAVAASPIIP 57
VP + + +++ V V+ + + PK V ADRI +AA
Sbjct: 66 TVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVG-ADRIVNCLAAYHKYG 124

Query: 58 D 58

Sbjct: 125 T 125


55KPHS_19940KPHS_19810Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_19940120-3.854962hypothetical protein
KPHS_19930149-17.126020hypothetical protein
KPHS_19920124-9.376440hypothetical protein
KPHS_19910021-7.845469putative TetR/AcrR family transcriptional
KPHS_19900-118-7.045895hypothetical protein
KPHS_19890-114-6.386383hypothetical protein
KPHS_19880-113-4.882467transposase
KPHS_19870-1100.163749putative TonB-dependent receptor
KPHS_198600120.820153hypothetical protein
KPHS_198500121.204618respiratory NADH dehydrogenase
KPHS_198400151.398906hypothetical protein
KPHS_198300160.753280beta-hexosaminidase
KPHS_198202170.532903putative beta-glucosidase
KPHS_198103170.843927putative fibronectin-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19910HTHTETR674e-16 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 67.0 bits (163), Expect = 4e-16
Identities = 31/167 (18%), Positives = 53/167 (31%), Gaps = 1/167 (0%)

Query: 3 DRDAALDKAMTLFWQHGYEATSLADLVEATGAKAPTLYAEFVNKEGLFRAVLDRYISRFA 62
R LD A+ LF Q G +TSL ++ +A G +Y F +K LF + + S
Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71

Query: 63 AKHEAVLFAEGKSVDRALRDYFTAVATCFTSKETPAGCFIINTSPALAASS-TDIANTIK 121
LR+ V ++E I + +
Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQR 131

Query: 122 SRHAMQEQALTQFLQQRQAQGELPAGRDVAQLAQFLNCVLQGMSISA 168
+ + Q L+ LPA + A + + G+ +
Sbjct: 132 NLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENW 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19810PERTACTIN280.027 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 28.1 bits (62), Expect = 0.027
Identities = 33/130 (25%), Positives = 45/130 (34%), Gaps = 21/130 (16%)

Query: 16 GCAGLREQPAPVEEAKPQPQQPAQPQPTVPTVPAVPSVPAQPGPIEHQDQQSGQPAPRVR 75
G + PAP +P PQ QP P P P P P + +Q PAP
Sbjct: 561 SLVGAKAPPAPKPAPQPGPQPGPQP-------PQPPQPPQPPQPPQPPQRQPEAPAP--- 610

Query: 76 HYDWNGAVQPLVGQMLQA---SGVNAGSILLVDSVNNRTNGSLNAGEATTALRSALAGNG 132
QP G+ L A + VN G + L ++ + +L+ L G
Sbjct: 611 --------QPPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRLNPDAGGAW 662

Query: 133 KFTLVSAQQL 142
QQL
Sbjct: 663 GRGFAQRQQL 672


56KPHS_19720KPHS_19600Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_19720219-0.7180603-oxoacyl-(acyl carrier protein) synthase II
KPHS_19710421-1.087977hypothetical protein
KPHS_19700420-1.4490813-ketoacyl-ACP reductase
KPHS_19690318-1.055170acyl carrier protein S-malonyltransferase
KPHS_19680216-1.8658183-oxoacyl-ACP synthase
KPHS_196704160.150808fatty acid/phospholipid synthesis protein
KPHS_196604170.76107250S ribosomal protein L32
KPHS_196502131.156409hypothetical protein
KPHS_196400122.751680putative inhibitor of septum formation
KPHS_19630-1122.79198723S rRNA pseudouridylate synthase C
KPHS_19620-2113.140040RNase E
KPHS_196102133.049547ribonuclease E
KPHS_196002152.137322putative LysR-family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19710TYPE4SSCAGA280.005 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 27.7 bits (61), Expect = 0.005
Identities = 16/48 (33%), Positives = 25/48 (52%)

Query: 10 KIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPD 57
++ G Q + QEE+ N F+E L ++ L +E+F TEI D
Sbjct: 380 QLTGSQRALSQEEIQNKIDFMEFLAQNNAKLDNLSEKEKEKFRTEIKD 427


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19700DHBDHDRGNASE1564e-49 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 156 bits (395), Expect = 4e-49
Identities = 91/250 (36%), Positives = 136/250 (54%), Gaps = 13/250 (5%)

Query: 4 EGKIALVTGASRGIGRAIAETLVARGAKVIGTATSESGAQAISDYLGANGK---GLMLNV 60
EGKIA +TGA++GIG A+A TL ++GA + + + + L A + +V
Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADV 66

Query: 61 TDPASIESVLENVRAEFGEVDILVNNAGITRDNLLMRMKDDEWNDIIETNLSSVFRLSKA 120
D A+I+ + + E G +DILVN AG+ R L+ + D+EW N + VF S++
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 121 VMRAMMKKRHGRIITIGSVVGTMGNAGQANYAAAKAGLIGFSKSLAREVASRGITVNVVA 180
V + MM +R G I+T+GS + A YA++KA + F+K L E+A I N+V+
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 181 PGFIETDMTRAL-----TDEQR-AGTLA----AVPAGRLGTPNEIASAVAFLASDEASYI 230
PG ETDM +L EQ G+L +P +L P++IA AV FL S +A +I
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 231 TGETLHVNGG 240
T L V+GG
Sbjct: 247 TMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19620IGASERPTASE498e-08 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 49.3 bits (117), Expect = 8e-08
Identities = 36/246 (14%), Positives = 67/246 (27%), Gaps = 8/246 (3%)

Query: 550 PVAAAAPVAAAAPAQPGLLSRFFSALKNIFSGAEEAKPAEVQIEKKAEEKPERQQERRKP 609
P + S + A PA + E E ++ K
Sbjct: 993 DTTNITTPNNIQADVPSVPSN--NEEIARVDEAPVPPPAPATPSETTETVAENSKQESKT 1050

Query: 610 RANNRRDRNDRRDNRDNRDNRDNRDNRDTRADNAEGREPRESREENRRNRREKPSQNVEA 669
N +D + + + N + E++E +E + +
Sbjct: 1051 VEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVE-KE 1109

Query: 670 RDVRQTSGDDAEKAKSRDEQQPRRERTRRRNDDKRQAQQEAKAQTREEPVVQETEQEERV 729
+ + E K + P++E++ A++ +EP Q +
Sbjct: 1110 EKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTE 1169

Query: 730 QTLPR-----RKPRQLAQKVRVESAVVEPVAEIVPEAVVAEVIAPHSEPVKAELPAGVES 784
Q +P + V ++VVE P V + S K V S
Sbjct: 1170 QPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRS 1229

Query: 785 VADQDE 790
V E
Sbjct: 1230 VPHNVE 1235



Score = 45.8 bits (108), Expect = 9e-07
Identities = 46/281 (16%), Positives = 80/281 (28%), Gaps = 30/281 (10%)

Query: 691 PRRERTRRRNDDKRQAQQEAKAQTREEPVVQETEQEERVQTLPRRKPRQLAQKVRVESAV 750
P E+ R + D Q V E+ RV P P S
Sbjct: 983 PEVEK-RNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAP-----ATPSET 1036

Query: 751 VEPVAEIVPEAVVAEVIAPHSEPVKAELPAGVESVADQDENGESREANGMPR------RS 804
E VAE + V + + + + + + N + + +
Sbjct: 1037 TETVAENSKQ-ESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKET 1095

Query: 805 RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPEMASGKVWIRYPVVRPQDQQPEE 864
+ + + ++ + + E TQ P++ S V P+ +Q E
Sbjct: 1096 QTTETKETATVEKEEKAKVETEKTQE---------VPKVTSQ--------VSPKQEQSET 1138

Query: 865 VQVQDASVAKTVEAVAAPVAVVETVTAAPVTVEPATMEPVTAEPVVVEPVAAAEPLVVDA 924
VQ Q + V +T T A +PV VV+
Sbjct: 1139 VQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVEN 1198

Query: 925 AEVVAPAAVEPAPQEPVTEAPAVEAPQAIAPVTLDPEPVVV 965
E PA +P + P +++ V + EP
Sbjct: 1199 PENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATT 1239



Score = 33.9 bits (77), Expect = 0.005
Identities = 44/271 (16%), Positives = 78/271 (28%), Gaps = 23/271 (8%)

Query: 724 EQEERVQTLPRRKPRQLAQKVRVESAVVEPVAEIVPEAVVAEVIAPHSEPVKAELPAGVE 783
E E+R QT+ +V EI A V E AP P A E
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEI---ARVDE--APVPPPAPATPSETTE 1038

Query: 784 SVADQDENGESREANGMPRRSRRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPEM 843
+VA+ + + + ++ V+ + + + VA + E
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKAN------TQTNEVAQSGSET 1092

Query: 844 ASGKVWIRYPVVRPQDQQPEEVQVQDASVAKTVEAVAAPVAVVETVTAAPVTVEPATMEP 903
+ + E+ + KT E + V TV+P EP
Sbjct: 1093 KETQ-----TTETKETATVEKEEKAKVETEKTQEV-PKVTSQVSPKQEQSETVQPQA-EP 1145

Query: 904 VTAEPVVVEPVAAAEPLVVDAAEVVAPAAVEPAPQEPVTEAPAVEAPQAIAPVTLDPEPV 963
V A ++PVTE+ V ++ + P
Sbjct: 1146 ARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPA 1205

Query: 964 VVEPEALER-----RLSLQRQLKPSPRSQKP 989
+P + +R ++ P + +P
Sbjct: 1206 TTQPTVNSESSNKPKNRHRRSVRSVPHNVEP 1236


57KPHS_19210KPHS_19080Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_19210-1153.269355choline dehydrogenase
KPHS_19200-1143.025368hypothetical protein
KPHS_19190-2153.019068major sodium/proline symporter
KPHS_19180-2173.852183proline
KPHS_19170-2152.743207trifunctional transcriptional regulator/proline
KPHS_19160-1132.401680hypothetical protein
KPHS_19150-1143.240262transcriptional regulator
KPHS_191400173.842518hypothetical protein
KPHS_19130-2164.906390pyrimidine utilization protein B
KPHS_191201195.905222hypothetical protein
KPHS_191100186.187017putative hydrolase
KPHS_191001165.295464putative hydrolase
KPHS_190902154.236001hypothetical protein
KPHS_190801143.669040putative flavin:NADH reductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19150HTHTETR654e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 65.0 bits (158), Expect = 4e-15
Identities = 30/166 (18%), Positives = 62/166 (37%), Gaps = 10/166 (6%)

Query: 10 GKRSQAVSAKKEAILAAALEAFSQFGIHGTRLEQVAERAGVSKTNLLYYYPSKEALYVAV 69
K Q ++ IL AL FSQ G+ T L ++A+ AGV++ + +++ K L+ +
Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62

Query: 70 LQQILAIWLAPLKAFREDI--SPLVAIREYIRLKLEVSRDHPQASKLF------CLEMLQ 121
+ + ++ PL +RE + LE + + +L E +
Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTV-TEERRRLLMEIIFHKCEFVG 121

Query: 122 GAPLLMGELTGDLKALVDEKSAIVSGWIDRGKL-APVDPQHLIFMI 166
++ D + I+ L A + + ++
Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_19130ISCHRISMTASE724e-17 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 72.4 bits (177), Expect = 4e-17
Identities = 44/184 (23%), Positives = 72/184 (39%), Gaps = 23/184 (12%)

Query: 4 LPARPESLTFAPQQSALIVVDMQNAYASQGGYLDLAGFDVSATRPVIDNINTAVAAARAA 63
+P S P ++ L++ DMQN + +D S + NI
Sbjct: 17 MPQNKVSWVPDPNRAVLLIHDMQNYF------VDAFTAGASPVTELSANIRKLKNQCVQL 70

Query: 64 GMLIIWFQNGWDDQYVEAGGPGSPNYHKSNALKTMRQRPELQGKLLAKGGWDYQLVDELT 123
G+ +++ PGS N L G L G ++ +++ EL
Sbjct: 71 GIPVVY-----------TAQPGSQNPDDRALLTDF------WGPGLNSGPYEEKIITELA 113

Query: 124 PQEGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGIV 183
P++ D+VL K RYS F T L ++R G L+ TGI ++ T + F +
Sbjct: 114 PEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFF 173

Query: 184 LEDA 187
+ DA
Sbjct: 174 VGDA 177


58KPHS_18470KPHS_18110Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_18470118-3.661583hypothetical protein
KPHS_18460020-4.186736nicotinate phosphoribosyltransferase
KPHS_18450024-4.839857putative peptidase
KPHS_18440-117-4.035184allantoate amidohydrolase
KPHS_18430018-4.268369putative transmembrane amino acid transporter
KPHS_18420221-3.699864diaminopropionate ammonia-lyase
KPHS_18410425-3.109662regulator for leucine (or lrp) regulon and
KPHS_18400225-1.611320asparaginyl-tRNA synthetase
KPHS_18390222-2.704785asparaginyl-tRNA synthetase
KPHS_18380016-2.291507OmpK35 protein
KPHS_183702140.053897outer membrane protein 1A/OmpK35 porin
KPHS_183601140.273385aromatic amino acid aminotransferase
KPHS_183502160.376683hypothetical protein
KPHS_183402150.212741hypothetical protein
KPHS_183302150.517238putative amidase
KPHS_183203151.062197condesin subunit B
KPHS_183100150.048511condesin subunit E
KPHS_183001160.809052chromosome segregation and condensation protein
KPHS_182901180.835878condesin subunit F
KPHS_182800161.868642putative metallothionein SmtA
KPHS_18270-1153.435051hypothetical protein
KPHS_182600141.730439chromosome partition protein MukF
KPHS_182501142.5059023-deoxy-manno-octulosonate cytidylyltransferase
KPHS_182401141.925345hypothetical protein
KPHS_182300160.387201hypothetical protein
KPHS_18220018-0.267337tetraacyldisaccharide 4'-kinase
KPHS_18210-117-0.503268lipid transporter ATP-binding protein/permease
KPHS_18200021-0.139542putative recombination protein with
KPHS_18190324-2.249664integration host factor subunit beta
KPHS_18180323-1.45477430S ribosomal protein S1
KPHS_18170-112-0.822159cytidylate kinase
KPHS_18160124-2.1447433-phosphoshikimate 1-carboxyvinyltransferase
KPHS_18150226-3.239258phosphoserine transaminase
KPHS_18140124-2.921774hypothetical protein
KPHS_18130126-3.351456hypothetical protein
KPHS_18120225-3.336937putative formate transporter
KPHS_18110224-2.411205formate acetyltransferase 1
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_18380ECOLIPORIN2017e-67 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 201 bits (512), Expect = 7e-67
Identities = 88/133 (66%), Positives = 104/133 (78%), Gaps = 2/133 (1%)

Query: 2 MKRNILAVVIPALLVAGAANAAEIYNKNGNKLDFYGKMVGEHVWTTNGDTSSDDTTYARI 61
MKR +LA+VIPALL AGAA+AAEIYNK+GNKLD YGK+ G H ++ + + D TY R+
Sbjct: 1 MKRKVLALVIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDD-SSKDGDQTYMRV 59

Query: 62 GLKGETQINDQLIGYGQWEYNMDASNVEGSQT-TKTRLAFAGLKAGEYGSFDYGRNYGAI 120
G KGETQINDQL GYGQWEYN+ A+ EG + TRLAFAGLK G+YGSFDYGRNYG +
Sbjct: 60 GFKGETQINDQLTGYGQWEYNVQANTTEGEGANSWTRLAFAGLKFGDYGSFDYGRNYGVL 119

Query: 121 YDVEAATDMLVEW 133
YDVE TDML E+
Sbjct: 120 YDVEGWTDMLPEF 132


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_18370ECOLIPORIN301e-104 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 301 bits (772), Expect = e-104
Identities = 134/252 (53%), Positives = 162/252 (64%), Gaps = 27/252 (10%)

Query: 2 GGDGWNYTDNYMTGRTNGVATYRNSDFFGLVDGLSFALQYQGKNDHDRA----------- 50
GGD + Y DNYMTGR NGVATYRN+DFFGLVDGL+FALQYQGKN+ A
Sbjct: 133 GGDSYTYADNYMTGRANGVATYRNTDFFGLVDGLNFALQYQGKNESQSADDVNIGTNNRN 192

Query: 51 ----IRKQNGDGFSTAATYAFDNGIALSAGYSSSNRSVDQKA----DGNGDKAEAWATSA 102
IR NGDGF + TY G + A Y++S+R+ +Q GDKA+AW
Sbjct: 193 NGDDIRYDNGDGFGISTTYDIGMGFSAGAAYTTSDRTNEQVNAGGTIAGGDKADAWTAGL 252

Query: 103 KYDANNIYAAVMYSQTYNMTP------EEDNHFAGKTQNFEAVVQYQFDFGLRPSIGYVQ 156
KYDANNIY A MYS+T NMTP D A KTQNFE QYQFDFGLRP++ ++
Sbjct: 253 KYDANNIYLATMYSETRNMTPYGKTDKGYDGGVANKTQNFEVTAQYQFDFGLRPAVSFLM 312

Query: 157 TKGKDLQSRAGFSGGDADLVKYIEVGTWYYFNKNMNVYAAYKFNQLDDND-YTKAAGVAT 215
+KGKDL + +G D DLVKY +VG YYFNKN + Y YK N LDD+D + K AG++T
Sbjct: 313 SKGKDL-TYNNVNGDDKDLVKYADVGATYYFNKNFSTYVDYKINLLDDDDPFYKDAGIST 371

Query: 216 DDQAAVGIVYQF 227
DD A+G+VYQF
Sbjct: 372 DDIVALGMVYQF 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_18320GPOSANCHOR474e-07 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 47.4 bits (112), Expect = 4e-07
Identities = 45/281 (16%), Positives = 95/281 (33%), Gaps = 20/281 (7%)

Query: 347 QEKIERYEADLDELQIRLEEQNEVVAEAVDRQEENEARAEAAELEVDELKSQLADYQQAL 406
+ K + L+ +E E ++ A ++ +N+ ++ EL+++ AD ++AL
Sbjct: 70 KLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKAL 129

Query: 407 DVQQTRAIQYNQALQALERAKALCHLPDLTPESADEWLETFQAKEQEATEKMLSLEQKMS 466
+ + + ++ LE KA L AD + + A + K+
Sbjct: 130 EGAMNFSTADSAKIKTLEAEKA-----ALAARKADL-----EKALEGAMNFSTADSAKIK 179

Query: 467 VAQTAHSQFEQAYQLVAAINGPLARNEAWDVARELLRDGVNQRHQAEQAQGLRSRLNELE 526
+ + E + L + A + A A+ +LE
Sbjct: 180 TLEAEKAALEARQA---ELEKALEGAMNFSTADSAKIKTLEAEKAALAAR-----KADLE 231

Query: 527 QRLREQQDAERQLAEFCKRQGKRYDIDDLETLHQELEARIASLADSVSNAQEQRMALRQE 586
+ L + + K + + LE ELE + + + + L E
Sbjct: 232 KALEGAMNFSTADSA--KIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAE 289

Query: 587 LEQLQSRTQTLMRRAPVWLAAQNSLNQLCEQSGEQFASGQE 627
L++ L ++ V A + SL + + S E +
Sbjct: 290 KAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEA 330



Score = 38.1 bits (88), Expect = 3e-04
Identities = 61/363 (16%), Positives = 117/363 (32%), Gaps = 29/363 (7%)

Query: 261 HLISEATNYVAADYMRHANERRIHLDKALEYRRDLFTSRSQLAAEQYKHVDMARELQEHN 320
+ E + + + + +L+ + K + L E
Sbjct: 53 EKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKA 112

Query: 321 GAEGDLEADY----QAASDHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAVD 376
+LEA +A +N + + +E +A L + LE+ E
Sbjct: 113 SKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFST 172

Query: 377 RQEENEARAEAAELEVDELKSQLADYQQALDVQQTRAIQYNQALQA-LERAKALCHLPDL 435
EA + ++ +++L + T + L+A A +
Sbjct: 173 ADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEK 232

Query: 436 TPESADEWLETFQAKEQEATEKMLSLEQKM-SVAQTAHSQFEQAYQLVAAINGPLARNEA 494
E A + AK + + +LE + + + + A I A A
Sbjct: 233 ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAA 292

Query: 495 WDVARELLRDGVNQRHQAEQAQGLRSRLNELEQRLREQQDAERQLAEFCK------RQGK 548
+ + L +Q A + Q LR L + + ++Q +AE Q E RQ
Sbjct: 293 LEAEKADLEH-QSQVLNANR-QSLRRDL-DASREAKKQLEAEHQKLEEQNKISEASRQSL 349

Query: 549 RYDID--------------DLETLHQELEARIASLADSVSNAQEQRMALRQELEQLQSRT 594
R D+D LE ++ EA SL + ++E + + + LE+ S+
Sbjct: 350 RRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKL 409

Query: 595 QTL 597
L
Sbjct: 410 AAL 412



Score = 37.4 bits (86), Expect = 5e-04
Identities = 42/307 (13%), Positives = 102/307 (33%), Gaps = 18/307 (5%)

Query: 935 QFEQLKEDYAYAQQTQRDARQQAFALAEVVQRRAHFSYSDSAEMLSGNSDLNEKLRQRLE 994
++ +Q + Q+ E+ SD + D N++L + L
Sbjct: 36 NTNEVSAVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELS 95

Query: 995 QAESERSRARDALRAHAAQLSQYNQVLASLKSSYDTKKELLNDLYKELQDIGVRADAGAE 1054
A+ + + +L A+++ + A L+ + + +++ + A A
Sbjct: 96 NAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAA 155

Query: 1055 ERA--RARRDELHMQLSNNRSRRNQLEKALTFCEAEMDNLTRKLRKLERDY-------CE 1105
+A + + + ++ LE EA L + L
Sbjct: 156 RKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKT 215

Query: 1106 MREQVVTAKAGWCAVMRLVKDNGVERRLHRRELAYLSAD------ELRSMSDKALGALRL 1159
+ + A + + ++ ++ L A+ + GA+
Sbjct: 216 LEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNF 275

Query: 1160 AVADNEHLRDVLRISEDPKRPERKIQFFVAVYQHLRERIRQDIIRTDDPVEAIEQMEIEL 1219
+ AD+ ++ + + + ++ V R+ +R+D+ D EA +Q+E E
Sbjct: 276 STADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL---DASREAKKQLEAEH 332

Query: 1220 SRLTEEL 1226
+L E+
Sbjct: 333 QKLEEQN 339



Score = 36.2 bits (83), Expect = 0.001
Identities = 51/327 (15%), Positives = 96/327 (29%), Gaps = 15/327 (4%)

Query: 782 ARENRIETLHAERESLSERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFEDDPEEEIRKL 841
A ++ + L E + E+ + + Q + E
Sbjct: 82 ALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADL------EKALEGAMNF 135

Query: 842 NSRRGELERALSAHESDNQQNRVQYEQAKEGVSALNRLLPRLNLLADDTLADRVDEIQER 901
++ + L A ++ + E+A EG + + A E
Sbjct: 136 STADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAEL 195

Query: 902 LDEAQEAARFIQQHGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYAQQTQRDARQQAFALA 961
+ A F ++ LE + L + E+ E + A
Sbjct: 196 EKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEK 255

Query: 962 EVVQRRAHFSYSDSAEMLSGNSDLNEKLRQRLEQAESERSRARDALRAHAAQLSQYNQVL 1021
++ R ++ + L G + + +++ E+E++ Q N
Sbjct: 256 AALEAR----QAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANR 311

Query: 1022 ASLKSSYDTKKELLNDLYKELQDIGVRADAGAEERARARRDELHMQLSNNRSRRNQLEKA 1081
SL+ D +E L E Q + + R RRD L +R + QLE
Sbjct: 312 QSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRD-----LDASREAKKQLEAE 366

Query: 1082 LTFCEAEMDNLTRKLRKLERDYCEMRE 1108
E + + L RD RE
Sbjct: 367 HQKLEEQNKISEASRQSLRRDLDASRE 393


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_18210ACRIFLAVINRP300.030 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 30.2 bits (68), Expect = 0.030
Identities = 14/74 (18%), Positives = 29/74 (39%), Gaps = 1/74 (1%)

Query: 128 ITYDSEQVASSSSSALITVVREGASIIGLFVMMFYYSWQLSLILIVLAPIVSVAIRVVSK 187
YD+ S ++ + E ++ L + +F + + +LI + P+V + +
Sbjct: 325 YPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILA 384

Query: 188 RFRNISKNMQNTMG 201
F S N G
Sbjct: 385 AF-GYSINTLTMFG 397


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_18190DNABINDINGHU1165e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 116 bits (293), Expect = 5e-38
Identities = 33/88 (37%), Positives = 57/88 (64%), Gaps = 1/88 (1%)

Query: 2 TKSELIERLASQQSHIPAKAVEDAVKEMLEHMASTLAQGERIEIRGFGSFSLHYRAPRTG 61
K +LI ++A + + + K AV + ++S LA+GE++++ GFG+F + RA R G
Sbjct: 3 NKQDLIAKVA-EATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKG 61

Query: 62 RNPKTGDKVELEGKYVPHFKPGKELRDR 89
RNP+TG++++++ VP FK GK L+D
Sbjct: 62 RNPQTGEEIKIKASKVPAFKAGKALKDA 89


59KPHS_17590KPHS_17120Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_17590123-3.178652oxygen-insensitive NADPH nitroreductase
KPHS_17580-121-0.107344oxygen-insensitive NADPH nitroreductase
KPHS_17570021-0.810826hypothetical protein
KPHS_17560122-1.657816glutaredoxin
KPHS_17550-117-1.860523hypothetical protein
KPHS_17540-114-2.286713hypothetical protein
KPHS_17530114-1.442444putative transport protein
KPHS_17520324-3.282980hypothetical protein
KPHS_17510223-2.788448prophage P2 Ogr protein
KPHS_17500123-2.245045Late control gene D protein (GpD)
KPHS_17490120-2.070618putative bacteriophage tail protein
KPHS_17480118-1.586072phage tail tape measure protein, family
KPHS_17470420-3.117936hypothetical protein
KPHS_17460421-3.704882hypothetical protein
KPHS_17450528-4.996833phage tail protein E
KPHS_17440434-7.393028phage major tail tube protein
KPHS_17430434-7.408585phage tail sheath protein
KPHS_17420338-7.731456hypothetical protein
KPHS_17410238-7.408993hypothetical protein
KPHS_17400334-6.569239hypothetical protein
KPHS_17390233-6.835283hypothetical protein
KPHS_17380035-7.190889tail protein I
KPHS_17370136-7.248582baseplate assembly protein J
KPHS_17360237-8.959994baseplate assembly protein W
KPHS_17350138-10.022086baseplate assembly protein V
KPHS_17330142-10.740477hypothetical protein
KPHS_17340138-8.481485hypothetical protein
KPHS_17320027-2.586383phage virion morphogenesis protein
KPHS_17310227-2.778617P2 phage tail completion protein R
KPHS_17300427-1.754890hypothetical protein
KPHS_17290426-1.341530hypothetical protein
KPHS_17280520-0.361592hypothetical protein
KPHS_17270619-0.565564hypothetical protein
KPHS_17260725-0.919578hypothetical protein
KPHS_17250623-1.002675hypothetical protein
KPHS_17240421-2.497382Head completion/stabilization protein (GpL)
KPHS_17230226-7.376135terminase, endonuclease subunit
KPHS_17220229-8.768332minor capsid protein H1 and H2, major capsid
KPHS_17210234-9.961389Presumed capsid scaffolding protein (GpO)
KPHS_17200138-11.446299terminase, ATPase subunit
KPHS_17190345-11.555209putative phage portal protein
KPHS_17180549-12.003522hypothetical protein
KPHS_17170429-5.039489hypothetical protein
KPHS_17160120-1.410617putative DinI-like damage-inducible protein
KPHS_17150018-1.034877hypothetical protein
KPHS_17140-114-0.100882putative reverse transcriptase/maturase
KPHS_171300100.479646integrase family protein
KPHS_171202152.671487putative DEOR-type transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17530TCRTETA320.003 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.5 bits (74), Expect = 0.003
Identities = 22/106 (20%), Positives = 35/106 (33%), Gaps = 6/106 (5%)

Query: 301 LMIGMITFQFSSFSFGIGNAAGLLFAGIML-GFLRANHPTFG-YIPQ--GALNMVKEFGL 356
L++ + +L+ G ++ G A G YI + FG
Sbjct: 76 LLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGF 135

Query: 357 MVFMAGVGLSAGAGINNGLGAVGGQM--LAAGLIVSLVPVVICFLF 400
M G G+ AG + +G AA + L + CFL
Sbjct: 136 MSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17480PREPILNPTASE320.005 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 32.5 bits (74), Expect = 0.005
Identities = 32/121 (26%), Positives = 44/121 (36%), Gaps = 29/121 (23%)

Query: 446 WLGNLASALDGFVKRHPQLTAALFKIAAVFAVVATAAGVVSLA------------LASIL 493
W G L + L GFV L A+ A + V+ + L L + L
Sbjct: 167 WGGLLFNLLGGFVS----LGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAAL 222

Query: 494 GPMAVLRVSAGILQLIFASAFGLVTRVIGGAGQAVIWLGRLMMANPI-----LAIVGLIA 548
G A L A + L+ +S G G +I L + PI LAI G IA
Sbjct: 223 G--AWLGWQALPIVLLLSSLVGAF------MGIGLILLRNHHQSKPIPFGPYLAIAGWIA 274

Query: 549 M 549
+
Sbjct: 275 L 275


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17120HTHTETR504e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 50.4 bits (120), Expect = 4e-10
Identities = 17/76 (22%), Positives = 33/76 (43%), Gaps = 2/76 (2%)

Query: 1 MAR--RPNDPQRRERILQATLDTIAAHGIHAVTHRKIATCANVPLGSLTYYFSGIEALIE 58
MAR + + R+ IL L + G+ + + +IA A V G++ ++F L
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 59 EAFSLFTAEMSAQYQQ 74
E + L + + +
Sbjct: 61 EIWELSESNIGELELE 76


60KPHS_16600KPHS_16550Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_16600312-0.059343ATP-dependent RNA helicase RhlE
KPHS_16590614-1.983675putative transketolase
KPHS_16580515-3.172406putative transketolase
KPHS_16570615-3.054348putative transketolase
KPHS_16560517-4.049535putative short-chain dehydrogenase/reductase
KPHS_16550317-2.847359putative general substrate transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16600SECA300.026 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.026
Identities = 20/67 (29%), Positives = 34/67 (50%), Gaps = 4/67 (5%)

Query: 246 QQVLVFTRTKHGANHLAEQLNKDGIRSAAIHG-NKSQGARTRALADFKSGGIRVLVATDI 304
Q VLV T + + ++ +L K GI+ ++ + A A A + + V +AT++
Sbjct: 450 QPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAA---VTIATNM 506

Query: 305 AARGLDI 311
A RG DI
Sbjct: 507 AGRGTDI 513


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16560DHBDHDRGNASE1182e-34 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 118 bits (296), Expect = 2e-34
Identities = 74/255 (29%), Positives = 119/255 (46%), Gaps = 16/255 (6%)

Query: 3 LKDKVAIITGAASARGLGFATAKLFAENGAKVVIIDLNGEAS---KTAAAALGEGHLGLA 59
++ K+A ITGAA +G+G A A+ A GA + +D N E ++ A
Sbjct: 6 IEGKIAFITGAA--QGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 60 ANVADEVQVQAAIEQILAKYGRVDVLVNNAGITQPLKLMDIKRANYDAVLDVSLRGTLLM 119
A+V D + +I + G +D+LVN AG+ +P + + ++A V+ G
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 120 SQAVIPTMRAQKSGSIVCISSVSAQRGGGIFGGPHYSAAKAGVLGLARAMARELGPDNVR 179
S++V M ++SGSIV + S A G Y+++KA + + + EL N+R
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181

Query: 180 VNCITPGLIQTDITAGKLTDD---------MTANILAGIPMNRLGDAIDIARAALFLGSD 230
N ++PG +TD+ D+ GIP+ +L DIA A LFL S
Sbjct: 182 CNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 231 LSSYSTGITLDVNGG 245
+ + T L V+GG
Sbjct: 242 QAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16550TCRTETA441e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 43.7 bits (103), Expect = 1e-06
Identities = 57/400 (14%), Positives = 128/400 (32%), Gaps = 50/400 (12%)

Query: 22 LTMIFLVYAINYADRTNIGAVLPFIIDEFHINNFEAGAIASMFFLGYAVSQIP----AGF 77
L +I A++ I VLP ++ + +N + L YA+ Q G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLAL-YALMQFACAPVLGA 65

Query: 78 FIAKRGTRGLVSLSIFGFSAFTWLMGTVSSVFGLKLVRLGLGLSEGPCPVGLASTINNWF 137
+ G R ++ +S+ G + +M T ++ L + R+ G++ V + I +
Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAV-AGAYIADIT 124

Query: 138 PPKEKATATGVYIAATMFAPIIVPPLAVWIAVTWGWRWVFFSFAIPGIVAAIAWYLLVKS 197
E+A G +++A ++ P+ + + FF+ A + + L+
Sbjct: 125 DGDERARHFG-FMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL-- 181

Query: 198 KPAESGFVSQSELAEINAGRESHNNSVR-ENILIAERFTWLDKIIRVKKMAPIDTAKGLF 256
ESH R + +A + +
Sbjct: 182 -------------------PESHKGERRPLRREALNPLASFRWARGMTVVAAL-----MA 217

Query: 257 TSKNILGDCLAYFMMVSVLYGLLTWIPLYLVKERGFDVMSMGFVASMPCIGGFIGAIGGG 316
+F+M V ++ +D ++G + G + ++
Sbjct: 218 V----------FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLA---AFGILHSLAQA 264

Query: 317 WVSDKLLGR-RRKPTMMFTAVSTVVMMLIMLNIPASTLAVCIGLFFVGFCLNIGWPAFTA 375
++ + R + +M ++ +++ +A I + IG PA A
Sbjct: 265 MITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASG--GIGMPALQA 322

Query: 376 YGMAVSDSKTYPIASSIINSGGNLGGFVAPMAAGFLLDKT 415
D + + + +L V P+ + +
Sbjct: 323 MLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAAS 362


61KPHS_16330KPHS_16030Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_163300144.678106sensor protein for basR
KPHS_163200144.212787excinuclease ABC subunit B
KPHS_16310-1125.144193hypothetical protein
KPHS_16300-1125.284899dithiobiotin synthetase
KPHS_162900134.497867biotin biosynthesis protein BioC
KPHS_16280-2124.2535518-amino-7-oxononanoate synthase
KPHS_16270-1153.359868biotin synthesis, sulfur insertion
KPHS_16260-1153.778485adenosylmethionine-8-amino-7-oxononanoate
KPHS_16250-1163.485666putative kinase inhibitor protein
KPHS_16240-1144.087567putative amino acid permease
KPHS_16230-2134.417269histidine ammonia lyase
KPHS_16220-1123.551115putative urocanase
KPHS_16210-1113.004101putative urocanase
KPHS_16200-291.270700histidine utilization repressor
KPHS_16190-1100.163099formimidoylglutamase
KPHS_16180-110-1.530800imidazolonepropionase
KPHS_16170-111-4.312209putative pectinesterase
KPHS_16160018-8.887373putative cation transport protein
KPHS_16150020-8.130114hypothetical protein
KPHS_16140124-7.965343hypothetical protein
KPHS_16130127-7.667946hypothetical protein
KPHS_16120126-7.649070hypothetical protein
KPHS_16110023-6.947804LuxR transcriptional regulator
KPHS_16100-118-3.955556putative regulatory protein MerR
KPHS_16090-117-3.444138hypothetical protein
KPHS_160800130.091635hypothetical protein
KPHS_160700131.267301hypothetical protein
KPHS_160600131.692536hypothetical protein
KPHS_160500131.8837376-phosphogluconolactonase
KPHS_160400132.450662putative phosphatase
KPHS_160301123.208603molybdenum transport ATP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16180UREASE371e-04 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 37.4 bits (87), Expect = 1e-04
Identities = 25/86 (29%), Positives = 37/86 (43%), Gaps = 16/86 (18%)

Query: 71 VRDGRIAAIVA------EDDV-----PSGRSIDLEGRLVTPGLIDCHTHLVFGGSRAQEW 119
++DGRIAAI + V P I EG++VT G +D H H + Q
Sbjct: 90 LKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEGKIVTAGGMDSHIHFI---CPQQIE 146

Query: 120 EQRLNGVSYQTISASGGGINSTVRAT 145
E ++G++ + G G AT
Sbjct: 147 EALMSGLT--CMLGGGTGPAHGTLAT 170


62KPHS_15860KPHS_15370Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_15860321-1.259342quinolinate synthetase
KPHS_15850425-2.021901hypothetical protein
KPHS_15840324-2.168191*******tol-pal system protein YbgF
KPHS_15830427-1.971017peptidoglycan-associated lipoprotein
KPHS_15820423-1.672970translocation protein TolB precursor
KPHS_15810924-2.366793TolA colicin import membrane protein
KPHS_15790023-2.472639colicin uptake protein TolR
KPHS_15780022-2.353547protein TolQ
KPHS_15800123-2.267456hypothetical protein
KPHS_15770-126-1.746245hypothetical protein
KPHS_15760029-1.393333riboflavin synthase subunit beta
KPHS_15750131-0.843431cytochrome d terminal oxidase polypeptide
KPHS_15740129-0.971026cytochrome d terminal oxidase, polypeptide
KPHS_15730228-0.851573hypothetical protein
KPHS_15720229-0.246559succinyl-CoA synthetase subunit alpha
KPHS_15710226-0.303095succinyl-CoA synthetase subunit beta
KPHS_15700223-0.495007dihydrolipoamide acetyltransferase
KPHS_15690120-1.066581alpha-ketoglutarate decarboxylase
KPHS_15680323-2.184851succinate dehydrogenase iron-sulfur subunit
KPHS_15660326-2.226947succinate dehydrogenase flavoprotein subunit
KPHS_15670019-3.782576hypothetical protein
KPHS_15650-115-3.010185succinate dehydrogenase cytochrome b556 small
KPHS_15640-115-2.217322hypothetical protein
KPHS_15630-114-1.095343type II citrate synthase
KPHS_156202151.137776hypothetical protein
KPHS_156102161.499144endonuclease 8
KPHS_156002162.141106pyroglutamyl-peptidase I
KPHS_155901152.429303hypothetical protein
KPHS_155800132.998452hypothetical protein
KPHS_15570-1123.250136hypothetical protein
KPHS_15560-1122.709650putative carboxylase
KPHS_15550-1123.204884putative carboxylase
KPHS_15540-1173.496981putative hydrolase-oxidase
KPHS_15530-1163.690321putative transport protein
KPHS_155200174.794978deoxyribodipyrimidine photolyase
KPHS_155100194.721707hypothetical protein
KPHS_155001184.518856K+-transporting ATPase
KPHS_15490-1163.993817potassium-transporting ATPase subunit B
KPHS_15480-2123.147625potassium-transporting ATPase subunit C
KPHS_15470-1122.731555sensor histidine kinase KdpD
KPHS_15460-2130.883711KDP operon transcriptional regulatory protein
KPHS_15450-2150.179370putative arginine/ornithine antiporter
KPHS_15440116-0.020570phosphoglucomutase
KPHS_15430217-1.962772replication initiation regulator SeqA
KPHS_15410117-1.905181hypothetical protein
KPHS_15420-115-2.255919esterase YbfF
KPHS_15400-117-5.925616LexA regulated protein
KPHS_15390017-4.737922flavodoxin
KPHS_15380020-2.957874ferric uptake regulator
KPHS_15370021-3.124002hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15840FLGMRINGFLIF280.048 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 28.0 bits (62), Expect = 0.048
Identities = 17/85 (20%), Positives = 30/85 (35%), Gaps = 8/85 (9%)

Query: 67 QTDIDSLRGQIQENQYQLNQIVER------QKQILLQIDSLSSGG--GAASGAQAPSSSG 118
+D + E Y N + Q I Q+ + GG GA S AP +
Sbjct: 266 TAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGALSNQPAPPNEA 325

Query: 119 DQSAAATSAAPAATSGAPAMTGDAN 143
+ T+ A + + + ++N
Sbjct: 326 PIATPPTNQQNAQNTPQTSTSTNSN 350


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15830OMPADOMAIN1167e-34 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 116 bits (291), Expect = 7e-34
Identities = 36/119 (30%), Positives = 55/119 (46%), Gaps = 4/119 (3%)

Query: 56 EEQARLQMQQLQQNNIVYFDLDKYDIRSDFAAMLDAHANFLRSN--PSYKVTVEGHADER 113
+Q + + V F+ +K ++ + A LD + L + V V G+ D
Sbjct: 205 APAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRI 264

Query: 114 GTPEYNIALGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDEAAYAKNRRAVL 172
G+ YN L ERRA +V YL KG+ AD+IS G+ P V G+ K R A++
Sbjct: 265 GSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNP-VTGN-TCDNVKQRAALI 321


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15810IGASERPTASE653e-13 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 65.1 bits (158), Expect = 3e-13
Identities = 29/233 (12%), Positives = 75/233 (32%), Gaps = 8/233 (3%)

Query: 68 QQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQ----Q 123
+ A + + AE +++ ++ + + Q +E AKEAK
Sbjct: 1021 DEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANT 1080

Query: 124 KQAEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADA-K 182
+ E A + + + + E KA E + + ++ + + ++ +
Sbjct: 1081 QTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQ 1140

Query: 183 KQAEAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAE 242
QAE A K+ +++ A Q E ++ + + ++ + E
Sbjct: 1141 PQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVE---QPVTESTTVNTGNSVVE 1197

Query: 243 KAAAEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAA 295
A + + + + ++ A ++ D++ A
Sbjct: 1198 NPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALC 1250



Score = 57.0 bits (137), Expect = 1e-10
Identities = 35/276 (12%), Positives = 72/276 (26%), Gaps = 7/276 (2%)

Query: 66 QQQQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQKQ 125
+Q++ EQ + Q E A E + + + + ++ E KE Q
Sbjct: 1043 NSKQESKTVEKNEQDATETTAQNRE----VAKEAKSNVKANTQTNEVAQSGSETKETQTT 1098

Query: 126 AEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKKQA 185
+ A KAK + E + K ++ K + QA D
Sbjct: 1099 ETKETATVEKEEKAKVET---EKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNI 1155

Query: 186 EAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAEKAA 245
+ ++ A + A+ + E + A Q +
Sbjct: 1156 KEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSES 1215

Query: 246 AEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKAAAAKKAA 305
+ K + + ++ + + A AKA
Sbjct: 1216 SNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNV 1275

Query: 306 AAKEADGVDNLLGDLSSGKNAPKTGGGAKGNNASAA 341
+ + L + N + N +S+
Sbjct: 1276 GKAVSQHISQLEMNNEGQYNVWVSNTSMNKNYSSSQ 1311



Score = 56.2 bits (135), Expect = 2e-10
Identities = 38/273 (13%), Positives = 81/273 (29%), Gaps = 7/273 (2%)

Query: 85 QQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQKQAEEAAAKAAAAAKAKADAQ 144
++ + + Q + + + ++ A A + + A+
Sbjct: 985 VEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAE-N 1043

Query: 145 AKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKKQAEAAAAKAAAEAKKQAEAEA 204
+K+ + K +A + A++A + A+ + A + E + E
Sbjct: 1044 SKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKET 1103

Query: 205 AKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAE--KAAAEKAAEKAAAQKAAAEK 262
A E + K E QE K Q + KQ +E + AE A E +
Sbjct: 1104 ATVEKEEKAKVETEK----TQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQ 1159

Query: 263 AAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKAAAAKKAAAAKEADGVDNLLGDLSS 322
+ A E+ A ++ E+ + + N
Sbjct: 1160 SQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKP 1219

Query: 323 GKNAPKTGGGAKGNNASAAGSGNTKNSASGADI 355
++ N A S N +++ + D+
Sbjct: 1220 KNRHRRSVRSVPHNVEPATTSSNDRSTVALCDL 1252



Score = 54.3 bits (130), Expect = 6e-10
Identities = 29/235 (12%), Positives = 71/235 (30%), Gaps = 4/235 (1%)

Query: 64 NRQQQQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQ 123
NR+ ++A + A + + Q E +E Q E + +E+E E K + +
Sbjct: 1065 NREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPK 1124

Query: 124 KQAEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKK 183
++ + + + QA+ A+E + EQ A + +++ ++
Sbjct: 1125 VTSQVSPKQEQSETVQP---QAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQ 1181

Query: 184 QAEAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAEK 243
+ + + A + +E++ K + ++ + A
Sbjct: 1182 PVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSS 1241

Query: 244 AAAEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKA 298
A ++ A A+ A A + +
Sbjct: 1242 NDRSTVAL-CDLTSTNTNAVLSDARAKAQFVALNVGKAVSQHISQLEMNNEGQYN 1295


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15680TCRTETOQM310.003 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 31.4 bits (71), Expect = 0.003
Identities = 11/41 (26%), Positives = 23/41 (56%), Gaps = 1/41 (2%)

Query: 14 VDDAPHMQDYTLEAEEGRDM-MLLDALIQLKEKDPSLSFRR 53
+++ + T+E + + MLLDAL+++ + DP L +
Sbjct: 339 IENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYV 379


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15530BACINVASINB290.036 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 29.3 bits (65), Expect = 0.036
Identities = 34/115 (29%), Positives = 58/115 (50%), Gaps = 4/115 (3%)

Query: 361 ILTLSARWSAAY-GQSSMPLMVLGLAVMGFAELFIDPVAMSQITRIEIPGVTGVLTGIYM 419
+LT+ + +A + G +S+ L +GLAVM E+ +S I + P + VL +
Sbjct: 324 LLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAATGVSFIQQALNPIMEHVLKPLME 383

Query: 420 LLSGAIANYLAGVIAD-QTSQASFDAAGAVNYSID--AYITVFSQITWGALACVG 471
L+ AI L G+ D +T++ + GA+ +I A I V + + GA A +G
Sbjct: 384 LIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMVAVIVVVAVVGKGAAAKLG 438


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15470PF06580300.047 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.8 bits (67), Expect = 0.047
Identities = 24/130 (18%), Positives = 48/130 (36%), Gaps = 28/130 (21%)

Query: 760 HIQLDLPDPLQLVHVDGPLFERVLINLLENAHKYAGAR----ASIGIRAEADARQLSLEV 815
+ + + V V P ++ L+EN K+ A+ I ++ D ++LEV
Sbjct: 241 QFENQINPAIMDVQV--PPM--LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEV 296

Query: 816 WDNGPGIPAGQEQTIFDKFARGNKESAIPGVGLGLA-ICQAIVDVHGG--TISASNRPEG 872
+ G ++ G GL + + + ++G I S + +G
Sbjct: 297 ENTGS----------------LALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEK-QG 339

Query: 873 GASFRVTLPG 882
+ V +PG
Sbjct: 340 KVNAMVLIPG 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_15460HTHFIS882e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.3 bits (219), Expect = 2e-22
Identities = 35/122 (28%), Positives = 57/122 (46%), Gaps = 1/122 (0%)

Query: 4 VLIIEDEHAIRRFLRTALEADGMRVFEAETLQRGLIEAATRKPDLAILDLGLPDGDGIDF 63
+L+ +D+ AIR L AL G V A DL + D+ +PD + D
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 64 IRDLRQ-WSQMPIIVLSARSEEHDKIAALDAGADDYLSKPFGIGELQARLRVALRRHGAA 122
+ +++ +P++V+SA++ I A + GA DYL KPF + EL + AL
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 123 QA 124
+
Sbjct: 126 PS 127


63KPHS_14910KPHS_14390Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_14910-312-3.937302nucleoside diphosphate kinase regulator
KPHS_14900-211-4.297302hypothetical protein
KPHS_14890-211-3.813282alkyl hydroperoxide reductase FAD/NAD(P)-binding
KPHS_14880-213-2.988651alkyl hydroperoxide reductase subunit C
KPHS_14870-114-2.413458thiol:disulfide interchange protein DsbG
KPHS_14860-214-1.902614putative LysR-family transcriptional regulator
KPHS_14850-3132.100748hypothetical protein
KPHS_14840-3122.268820hypothetical protein
KPHS_14830-1143.405002putative aminotransferase
KPHS_14820-1143.8013582,3-diketo-5-methylthio-1-phosphopentane
KPHS_148100154.5986211,2-dihydroxy-3-keto-5-methylthiopentene
KPHS_148001165.319372biotin sulfoxide reductase
KPHS_147900165.386457sugar ABC transporter periplasmic sugar-binding
KPHS_147802197.271565putative ABC-type sugar transport system ATPase
KPHS_147702196.785788hypothetical protein
KPHS_147601186.157977putative inner-membrane translocator
KPHS_147502185.889309putative kinase
KPHS_147403195.589122D-isomer specific 2-hydroxyacid dehydrogenase
KPHS_147302205.546347putative carbohydrate kinase
KPHS_147202214.955539hypothetical protein
KPHS_147101224.738048cytosine permease
KPHS_147001205.061442DNA-binding protein
KPHS_14690-1185.449723putative cytosine deaminase-related
KPHS_14680-2185.358946putative periplasmic binding protein/LacI
KPHS_14670-2163.787383putative inositol monophosphatase
KPHS_14660-2132.291377hypothetical protein
KPHS_14650-1132.262967putative 3-phytase
KPHS_14640-2131.257289S-methyl-5-thioribose-1-phosphate isomerase
KPHS_14630-2140.499930methylthioribose kinase
KPHS_14620-1150.693270hypothetical protein
KPHS_14610-1152.675214hypothetical protein
KPHS_146000173.983485hypothetical protein
KPHS_14590-1163.316654putative periplasmic binding protein/LacI
KPHS_14580-2142.897638inner-membrane translocator
KPHS_14570-2132.611499putative ABC transport system ATPase component
KPHS_145602141.758960putative alcohol dehydrogenase
KPHS_145501161.079033putative short-chain alcohol dehydrogenase
KPHS_14540-1171.314197hypothetical protein
KPHS_14530-1192.368705Xre family transcriptional regulator
KPHS_14520-2192.977858hypothetical protein
KPHS_14510-2183.693192carbon starvation protein
KPHS_14500-1184.530174hypothetical protein
KPHS_14490-1185.0004092,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
KPHS_14480-1185.5794822,3-dihydro-2,3-dihydroxybenzoate synthetase
KPHS_144700186.459117enterobactin synthase subunit E
KPHS_144601187.353198isochorismate synthase
KPHS_144503218.450136iron-enterobactin transporter periplasmic
KPHS_144404218.735094enterobactin exporter EntS
KPHS_144303208.923347ferric enterobactin transport protein
KPHS_144200216.916631iron complex transport system permease
KPHS_144100206.635728iron-enterobactin transporter ATP-binding
KPHS_14400-1196.338181ATP-dependent serine activating enzyme
KPHS_14390-1153.524933invasin-like protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14700HTHFIS371e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 36.7 bits (85), Expect = 1e-04
Identities = 15/32 (46%), Positives = 22/32 (68%)

Query: 345 LETLLQENGNVVRAADRLGLHRNTLHQRIQRI 376
L L GN ++AAD LGL+RNTL ++I+ +
Sbjct: 442 LAALTATRGNQIKAADLLGLNRNTLRKKIREL 473


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14660TCRTETA371e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 36.7 bits (85), Expect = 1e-04
Identities = 53/313 (16%), Positives = 106/313 (33%), Gaps = 26/313 (8%)

Query: 99 LGLLLSAGMNLMMGMTTNALLLAIFWGINGWAQSMGVGPCAVSLARWYGVKERGTFYGIW 158
+ L +A +M +L I + G + G A +A ER +G
Sbjct: 78 VSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAY-IADITDGDERARHFGFM 136

Query: 159 STAHNIGEAVTYMVIAAVIAGFGWQMGYLSTAALGAAGVVLLVLFMHDSPQSSGFPSINV 218
S G V V+ ++ GF + + AAL + + +S + P
Sbjct: 137 SACFGFG-MVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRP---- 191

Query: 219 IRDEPQEEVEARGSVFKNQLLALRNPALWTLALASAFMYIDRYAVNSWGIFFLEQDKAYS 278
EA + + +A+ + + W I F E +
Sbjct: 192 ------LRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVI-FGEDRFHWD 244

Query: 279 TLEASGIIGVN-AIAGIAGTIIAGMLSDRF---FPRNRSVMAGFISLLNTAGFALMLWSP 334
IG++ A GI ++ M++ R++M G I+ + G+ L+ ++
Sbjct: 245 A----TTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIA--DGTGYILLAFAT 298

Query: 335 HNYYTDILAMIIFGATIGALTCFLGGLIAVDISSRKAAGAALGTIGIASYAGAGLGEFLT 394
+ M++ A+ G L +++ + + G G++ + + +G L
Sbjct: 299 R-GWMAFPIMVLL-ASGGIGMPALQAMLSRQVDEER-QGQLQGSLAALTSLTSIVGPLLF 355

Query: 395 GIIIDKTAILENG 407
I + NG
Sbjct: 356 TAIYAASITTWNG 368


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14550DHBDHDRGNASE1355e-41 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 135 bits (341), Expect = 5e-41
Identities = 87/253 (34%), Positives = 130/253 (51%), Gaps = 15/253 (5%)

Query: 5 LTGKKALVTGASRGLGRAIALSLARAGADVVITYEKSVDKAQAVADEIKALGRYGEAVQA 64
+ GK A +TGA++G+G A+A +LA GA + + + +K + V +KA R+ EA A
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPA 64

Query: 65 DSASAQAIQDAVTHAARSLGGLDILVNNAGIARGGPLESMTLADIDALINVNIRGVVIAT 124
D + AI + R +G +DILVN AG+ R G + S++ + +A +VN GV A+
Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNAS 124

Query: 125 QEALVHMAD--GGRIINIGSCLANRVAMPGIAVYAMTKSALNALTRGLARDLGPRGITVN 182
+ +M D G I+ +GS A +A YA +K+A T+ L +L I N
Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRT-SMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 183 LVHPGPTNSDMN-----PEDGEQ------AEAQRQMIAVGHYGQPEDIAAAVTFLASPAA 231
+V PG T +DM E+G + E + I + +P DIA AV FL S A
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 232 GQISGTGLDVDGG 244
G I+ L VDGG
Sbjct: 244 GHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14510ACRIFLAVINRP310.014 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 31.3 bits (71), Expect = 0.014
Identities = 9/60 (15%), Positives = 25/60 (41%), Gaps = 1/60 (1%)

Query: 172 VIILAVLAMIVVKALTHSPWG-TYTVAFTIPLAIFMGIYIRYLRPGRIGEVSVIGLVMLV 230
++ ++ + + + A + W +V +PL I + L + ++GL+ +
Sbjct: 875 LVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTI 934


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14490DHBDHDRGNASE340e-121 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 340 bits (873), Expect = e-121
Identities = 109/265 (41%), Positives = 151/265 (56%), Gaps = 20/265 (7%)

Query: 1 MAALDFRGQTVWVTGAGKGIGYATALAFVEAGANVTGFD---------------LAFDGE 45
M A G+ ++TGA +GIG A A GA++ D A E
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 46 SYPFATETLDVADADQVREACSRLLANTERLDVLVNAAGILRMGATDQLSAEDWQQTFAV 105
++P DV D+ + E +R+ +D+LVN AG+LR G LS E+W+ TF+V
Sbjct: 61 AFP-----ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSV 115

Query: 106 NVGGAFNLFQQTMAQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALTVGLEL 165
N G FN + +R G+IVTV S+ A PR M+AY +SKAA +GLEL
Sbjct: 116 NSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLEL 175

Query: 166 AGSGVRCNLVSPGSTDTDMQRTLWVSDDAEQQRIRGFGEQFKLGIPLGKIARPQEIANTI 225
A +RCN+VSPGST+TDMQ +LW ++ +Q I+G E FK GIPL K+A+P +IA+ +
Sbjct: 176 AEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAV 235

Query: 226 LFLASSHASHITLQDIVVDGGSTLG 250
LFL S A HIT+ ++ VDGG+TLG
Sbjct: 236 LFLVSGQAGHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14480ISCHRISMTASE426e-154 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 426 bits (1096), Expect = e-154
Identities = 152/303 (50%), Positives = 201/303 (66%), Gaps = 20/303 (6%)

Query: 1 MAIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANI 60
MAIP +Q Y +P ASD+P NKV+W +P+RA LLIHDMQ YF++ + ++ + ++ ANI
Sbjct: 1 MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI 60

Query: 61 AALRDFCKQNGIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTV 120
L++ C Q GIPV YTAQP Q+ +DRALL D WGPGL P ++++I LAP++DD V
Sbjct: 61 RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV 120

Query: 121 LVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTTATDAFMRDIKPFFVADALAD 180
L KWRYSAF R+ L EM+++ GRDQLIITG+YAHIGC+ TA +AFM DIK FFV DA+AD
Sbjct: 121 LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD 180

Query: 181 FSREEHLMALKYVAGRSGRVVMTEELL--------PIPVSKA-----------ALRALIL 221
FS E+H MAL+Y AGR VMT+ LL + + A +R I
Sbjct: 181 FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA 240

Query: 222 PLLDESDEPLD-DENLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLTR 280
LL E+ E + E+L+D GLDSVR+M L +WR+ ++ FV LA+ PTI+ W LLT
Sbjct: 241 ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTT 300

Query: 281 EVQ 283
Q
Sbjct: 301 RSQ 303


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14450FERRIBNDNGPP511e-09 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 51.5 bits (123), Expect = 1e-09
Identities = 59/288 (20%), Positives = 99/288 (34%), Gaps = 31/288 (10%)

Query: 40 HTLPSQPLRIVSTSVTLTGSLLAIDAPVVASGATTPNNRVADSQGFLRQWSEVAKARKLA 99
H P RIV+ LLA+ VAD+ + SE +
Sbjct: 29 HAAAIDPNRIVALEWLPVELLLALGIVPYG---------VADTINYRLWVSEPPLPDSV- 78

Query: 100 RLYIG---EPSAEAVAAQMPDLILVSATGGDSALPLYDQLKTIAPTLVINYDDKS----- 151
+ +G EP+ E + P ++ SA G P + L IAP N+ D
Sbjct: 79 -IDVGLRTEPNLELLTEMKPSFMVWSAGYG----PSPEMLARIAPGRGFNFSDGKQPLAM 133

Query: 152 WQTLLTQLGQITGHEQQASARIADFNKQLVSLKEKMKLPPQPVTALVYTAAAHSANIWTP 211
+ LT++ + + A +A + + S+K + L ++ P
Sbjct: 134 ARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGP 193

Query: 212 ESAQGQMLQQLGFSLATLPGGLPASHSQGKRHDIVQLGGENLAAGLNGQSLFLFAGDQKD 271
S ++L + G A + + + LAA + L + KD
Sbjct: 194 NSLFQEILDEYGIP--------NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKD 245

Query: 272 ADAIYANPLLAHLPAVAGKRVYPLGTETFRLDYYSALLVLQRLSSLFG 319
DA+ A PL +P V R + F SA+ ++ L + G
Sbjct: 246 MDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVLDNAIG 293


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14440TCRTETB354e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 35.2 bits (81), Expect = 4e-04
Identities = 39/187 (20%), Positives = 71/187 (37%), Gaps = 8/187 (4%)

Query: 24 IARFISILSLGLLGVAIPVQIQMMTHSTWQVGLSVTLTGASMFVGLMVGGVLADRYKRKR 83
I F S+L+ +L V++P T + +G V G L+D+ KR
Sbjct: 21 ILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKR 80

Query: 84 LILLARGTCGVGFVGLCLNALLPEPSLAAIYLLGIWDGFFASLGVTALLAATPALVGREN 143
L+L G V + + A ++ G F +L ++ + +EN
Sbjct: 81 LLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPAL----VMVVVARYIPKEN 136

Query: 144 LMQAGAITMLTVRLGSVISPMIGGLLLATGGVAWNFGLAAAGTFITTLTLLRLPQLPPPP 203
+A + V +G + P IGG++ + W++ L IT +T+ L +L
Sbjct: 137 RGKAFGLIGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIP--MITIITVPFLMKLLKKE 192

Query: 204 QPREHPL 210
+
Sbjct: 193 VRIKGHF 199


64KPHS_14270KPHS_13940Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_142700193.927814ribose transport system permease
KPHS_142601182.985993short-chain dehydrogenase/reductase SDR
KPHS_142501171.3833593-oxoacyl-(acyl-carrier-protein) reductase
KPHS_14240-1180.818858putative S-transferase
KPHS_14230-1170.522330hypothetical protein
KPHS_14220-1171.189437glycine cleavage system transcriptional
KPHS_142100160.784077small-conductance mechanosensitive ion channel
KPHS_14200-1171.891927small-conductance mechanosensitive ion channel
KPHS_14190-1203.194994inhibitor of vertebrate lysozyme precursor
KPHS_14180-2192.961819choline transport protein BetT
KPHS_14170-2183.898882transcriptional regulator BetI
KPHS_14160-2184.452202NAD+-dependent betaine aldehyde dehydrogenase
KPHS_14150-1184.999049choline dehydrogenase
KPHS_14140-1175.120334putative GCN5-related N-acetyltransferase
KPHS_141300175.252428putative ABC transport system periplasmic
KPHS_141202196.577059putative ABC transporter permease
KPHS_141102206.495675putative ABC transport system permease
KPHS_141001195.019168putative ABC transport system oligopeptide
KPHS_14090-2202.523639peptide/opine/nickel ABC superfamily ATP binding
KPHS_14080-2182.002386hypothetical protein
KPHS_14070-1180.670286hypothetical protein
KPHS_14060-1170.772744putative oxidoreductase
KPHS_14050-2161.618507putative ABC transporter
KPHS_14040-1152.460873putative binding-protein-dependent transport
KPHS_140300143.712482putative binding-protein-dependent transport
KPHS_14020-1153.780812amino acid ABC transporter substrate-binding
KPHS_140100143.467909GntR family transcriptional regulator
KPHS_14000-2161.648911putative aminotransferase
KPHS_13990-1183.107637hypothetical protein
KPHS_13980-1163.414090carboxylate-amine ligase
KPHS_13970-1173.607699hypothetical protein
KPHS_13960-1173.213326hypothetical protein
KPHS_13950-1174.163163hypothetical protein
KPHS_139401204.819512putative acridine efflux pump
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14260DHBDHDRGNASE1322e-39 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 132 bits (332), Expect = 2e-39
Identities = 75/262 (28%), Positives = 118/262 (45%), Gaps = 12/262 (4%)

Query: 3 RDFQNKTVVITGACRGIGAGIAERFARDGANLVMV---SNAERVHETAETLRQRYQADIL 59
+ + K ITGA +GIG +A A GA++ V ++ R+
Sbjct: 4 KGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE--- 60

Query: 60 SLQVDVTDEAQVQGLYEQAAARFGTIDVSIQNAGVITIDYYDRMPKADFEKVLAVNTTGV 119
+ DV D A + + + G ID+ + AGV+ + ++E +VN+TGV
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 120 WLCCREAAKYMVKQNHGSLINTSSGQGRQGFIYTPHYAASKMGVIGITQSLAHELAPWNI 179
+ R +KYM+ + GS++ S YA+SK + T+ L ELA +NI
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 180 TVNAFCPGIIESEMWDYNDRVWGEILSTEQKRYGKGELMAEWVEGIPMKRAGKPEDVAGL 239
N PG E++M +W + EQ G E + GIP+K+ KP D+A
Sbjct: 181 RCNIVSPGSTETDM---QWSLWADENGAEQVIKGSLE---TFKTGIPLKKLAKPSDIADA 234

Query: 240 VAFLASDDARYLTGQTINIDGG 261
V FL S A ++T + +DGG
Sbjct: 235 VLFLVSGQAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14250DHBDHDRGNASE1309e-39 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 130 bits (327), Expect = 9e-39
Identities = 78/251 (31%), Positives = 129/251 (51%), Gaps = 12/251 (4%)

Query: 16 RVAFVTGAGSGIGQTIACSLASAGARVVCFDLRDDGGLAETVSHIESIGGQACSYNGDVR 75
++AF+TGA GIG+ +A +LAS GA + D + L + VS +++ A ++ DVR
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEK-LEKVVSSLKAEARHAEAFPADVR 67

Query: 76 QIADLRAAVALAKSRYGRLDIAVNAAGIANANPALEMESEQWQRVIDINLTGVWNSCKAE 135
A + A + G +DI VN AG+ + E+W+ +N TGV+N+ ++
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 136 AELMLESGGGSIINIASMSGIIVNRGLDQAHYNCSKAGVIHLSKSLAMEWVGKGIRVNSI 195
++ M++ GSI+ + S + + A Y SKA + +K L +E IR N +
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSM--AAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 196 SPGYTATPM--------NTRPEMVHQTRE-FESQTPMQRMAKVEEMAGPALFLASDAASF 246
SPG T T M N +++ + E F++ P++++AK ++A LFL S A
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 247 CTGVDLVVDGG 257
T +L VDGG
Sbjct: 246 ITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14170HTHTETR645e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 64.3 bits (156), Expect = 5e-15
Identities = 31/172 (18%), Positives = 58/172 (33%), Gaps = 15/172 (8%)

Query: 10 RRRQLIDATLDAINEVGMHDATIAQIARRAGVSTGIISHYFKDKNGLLEATMRDITSQLR 69
R+ ++D L ++ G+ ++ +IA+ AGV+ G I +FKDK+ L S +
Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71

Query: 70 DAVLNRLHALPDGSASQRLQAIVGGNFDETQISSAAMKAWLAFWASSMHQP-------ML 122
+ L P L+ I+ + T ++ + +
Sbjct: 72 ELELEYQAKFPGDPL-SVLREILIHVLEST-VTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 123 YRLQQVSSRRLLSNLVYEFRRE---LPREQAQEAGYGLAALIDGL---WLRA 168
R + S + + + A + I GL WL A
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFA 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14060DHBDHDRGNASE842e-21 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 83.9 bits (207), Expect = 2e-21
Identities = 52/187 (27%), Positives = 84/187 (44%), Gaps = 5/187 (2%)

Query: 6 VVFITGATSGFGEAAAQVFADAGWSLVLSGRRYPRLKALQ--DRLAARVPVHIIELDVRD 63
+ FITGA G GEA A+ A G + +L+ + + AR DVRD
Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHA-EAFPADVRD 68

Query: 64 SEAVAAAVASLPADFADITTLINNAGLALSPLPAQEVALEDWKTMIDTNVTGLVTVTHAL 123
S A+ A + + I L+N AG+ L P ++ E+W+ N TG+ + ++
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGV-LRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 124 LPTLIRHGAGASIINIGSIAGQWPYPGSHVYGASKAFVKQFSYNLRCDLLGTGVRVTDLA 183
++ +G SI+ +GS P Y +SKA F+ L +L +R ++
Sbjct: 128 SKYMMDRRSG-SIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 184 PGIAETE 190
PG ET+
Sbjct: 187 PGSTETD 193


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13940ACRIFLAVINRP9140.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 914 bits (2363), Expect = 0.0
Identities = 416/1034 (40%), Positives = 607/1034 (58%), Gaps = 17/1034 (1%)

Query: 1 MLTFFIRRPRFAMVIALLLTFVGAVSLKLIPVEQYPAITPPVVNVSASWPGASASDVAEA 60
M FFIRRP FA V+A++L GA+++ +PV QYP I PP V+VSA++PGA A V +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 IAAPLETQLNGVDHMLYMESTSSDEGTYRLSITFAAGTDADLAAIDVQNRVAQALAQLPA 120
+ +E +NG+D+++YM STS G+ +++TF +GTD D+A + VQN++ A LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQNGVQVRKRASNLLMGVSLYSPLGTLTPLFVSNYASTQVREALARLPGVGEVQMFGA 180
EVQQ G+ V K +S+ LM S T +S+Y ++ V++ L+RL GVG+VQ+FGA
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 RDYSMRIWLRPDRMNALNITTDDVAQALREQNVQGAAGQVGTPPVFNGQQQTLTINGLGR 240
+ Y+MRIWL D +N +T DV L+ QN Q AAGQ+G P GQQ +I R
Sbjct: 181 Q-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 241 LNEAASFGEIIIRRGAQGQLVRLADVATIELGARSYSSGAQLNGKASAYLGIYPTPTANA 300
FG++ +R + G +VRL DVA +ELG +Y+ A++NGK +A LGI ANA
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 301 LQVASAVRAELNRLHTRFPADLTWEVKFDTTRFVAATIKEIGVSLALTLLAVVVVVSLFL 360
L A A++A+L L FP + +DTT FV +I E+ +L ++ V +V+ LFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 361 QSWRATLIVVLAIPVSLIGTFAVLYLLGYSANTLSLFAIILALTMVVDDAIVVVENVETK 420
Q+ RATLI +A+PV L+GTFA+L GYS NTL++F ++LA+ ++VDDAIVVVENVE
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 421 MAE-GLDRLQATAQALRQIAGPVIATTLVLLAVFVPVALLPGIVGELYRQFAVTLSTAVA 479
M E L +AT +++ QI G ++ +VL AVF+P+A G G +YRQF++T+ +A+A
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 480 LSSLVALTLTPALCALLLRPRPARP----AAVWRAFNRLLDGTRDGYGRLVGRMNRRPWL 535
LS LVAL LTPALCA LL+P A + FN D + + Y VG++
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 536 ALAATVAAGALVAFSFTSMPKGFLPQEDQGYLFASVQLPEAASLERTEAVMAQARKLLMA 595
L A + F +P FLP+EDQG +QLP A+ ERT+ V+ Q +
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 596 NPA--VEDVIQVSGFNILNGTSASNGGFISVMLKDWHQRPP----LDAVMADIQRQLLSL 649
N VE V V+GF+ A N G V LK W +R +AV+ + +L +
Sbjct: 600 NEKANVESVFTVNGFSF--SGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKI 657

Query: 650 PEATIMTFAPPTLPGLGNASGFDLRILAQAGQSSAELEQVTREILQLANQH-SQLSRVFT 708
+ ++ F P + LG A+GFD ++ QAG L Q ++L +A QH + L V
Sbjct: 658 RDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRP 717

Query: 709 TWSSNVPQLTLTVDRDRAALLDVPVAQIFSSLQTAFGGTRAGDFSRNNRVYHVVMQNEMQ 768
+ Q L VD+++A L V ++ I ++ TA GGT DF RV + +Q + +
Sbjct: 718 NGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAK 777

Query: 769 WRERAEQISELYVRSRDGERVRLSNLVTITPTVGAPFIQQYNQFPSVSVSGSAAEGVSSR 828
+R E + +LYVRS +GE V S T G+P +++YN PS+ + G AA G SS
Sbjct: 778 FRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG 837

Query: 829 TAMAAMEQILQAHLPPGYDYAWSGISWQEQQTGNQAVWIVLAAVAMAWLFLVAQYESWTL 888
AMA ME + LP G Y W+G+S+QE+ +GNQA +V + + +L L A YESW++
Sbjct: 838 DAMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSI 896

Query: 889 PASVMLSVLFAIGGALLWLWTAGYANDVYVQIGLVLLIALAAKNAILIVEFARSRRE-EG 947
P SVML V I G LL NDVY +GL+ I L+AKNAILIVEFA+ E EG
Sbjct: 897 PVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 948 LSIVDAAREGATRRFRAVMMTAVSFIIGIMPMMLATGAGAQSRRIIGTTVFSGMLVATMV 1007
+V+A R R ++MT+++FI+G++P+ ++ GAG+ ++ +G V GM+ AT++
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 1008 GILFIPSLYVLFQR 1021
I F+P +V+ +R
Sbjct: 1017 AIFFVPVFFVVIRR 1030



Score = 75.6 bits (186), Expect = 7e-16
Identities = 88/521 (16%), Positives = 180/521 (34%), Gaps = 43/521 (8%)

Query: 531 RRPWLALAATVAAGALVAFSFTSMPKGFLPQEDQGYLFASVQLPEAASLERTEAVMAQAR 590
RRP A + A + +P P + S P A + + V
Sbjct: 7 RRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV----- 61

Query: 591 KLLMANPAVEDVIQVSGFNILNGTSASNGGFISVMLKDWHQRPPLDA---VMADIQRQLL 647
+++ + ++ TS S G +++ L P A V +Q
Sbjct: 62 ----TQVIEQNMNGIDNLMYMSSTSDSAGS-VTITLTFQSGTDPDIAQVQVQNKLQLATP 116

Query: 648 SLPEATIMTFAPPTLPGLGNASGFDLRILAQAGQSSAELEQVTREILQLANQHSQLSRV- 706
LP+ + ++S + + + + Q +N LSR+
Sbjct: 117 LLPQE----VQQQGISVEKSSSSYLMVAGFVS--DNPGTTQDDISDYVASNVKDTLSRLN 170

Query: 707 ----FTTWSSNVPQLTLTVDRDRAALLDVPVAQIFSSLQTAFGGTRAGDF------SRNN 756
+ + + + +D D + + + L+ AG
Sbjct: 171 GVGDVQLFGAQY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQ 229

Query: 757 RVYHVVMQNEMQWRERAEQISELYVR-SRDGERVRLSNLVTITPTVGA-PFIQQYNQFPS 814
++ Q + E+ ++ +R + DG VRL ++ + I + N P+
Sbjct: 230 LNASIIAQTRFK---NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPA 286

Query: 815 VSVSGSAAEGVSSR-TAMAAMEQI--LQAHLPPG--YDYAWSGISWQEQQTGNQAVWIVL 869
+ A G ++ TA A ++ LQ P G Y + + + ++ V +
Sbjct: 287 AGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSI-HEVVKTLF 345

Query: 870 AAVAMAWLFLVAQYESWTLPASVMLSVLFAIGGALLWLWTAGYANDVYVQIGLVLLIALA 929
A+ + +L + ++ ++V + G L GY+ + G+VL I L
Sbjct: 346 EAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLL 405

Query: 930 AKNAILIVE-FARSRREEGLSIVDAAREGATRRFRAVMMTAVSFIIGIMPMMLATGAGAQ 988
+AI++VE R E+ L +A + ++ A++ A+ +PM G+
Sbjct: 406 VDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGA 465

Query: 989 SRRIIGTTVFSGMLVATMVGILFIPSLYVLFQRMREWAHRR 1029
R T+ S M ++ +V ++ P+L + H
Sbjct: 466 IYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHE 506


65KPHS_13840KPHS_13650Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_13840019-3.450940putative transport protein, PTS system
KPHS_13830121-5.126537putative transport protein, PTS system
KPHS_13820121-4.688806putative inner membrane protein
KPHS_13810118-3.684918putative transport protein, PTS system
KPHS_13800116-2.538267putative inner membrane protein
KPHS_13790112-0.456684putative phosphosugar isomerase
KPHS_13780013-0.359613putative inner membrane protein
KPHS_137701163.352761putative inner membrane protein
KPHS_137600152.911723putative permease
KPHS_137500142.303377hypothetical protein
KPHS_13740011-1.380495major facilitator superfamily transporter MFS_1
KPHS_13730-217-4.936503putative transport
KPHS_13720026-6.984198putative LacI family regulatory protein
KPHS_13710128-9.430693NUDIX hydrolase
KPHS_13700224-8.795167hypothetical protein
KPHS_13690225-8.878842RmbA
KPHS_13680223-8.302071hypothetical protein
KPHS_13670225-8.930816hypothetical protein
KPHS_13660222-7.988088hypothetical protein
KPHS_13650-120-5.012762hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13780HTHFIS691e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 69.5 bits (170), Expect = 1e-14
Identities = 34/136 (25%), Positives = 66/136 (48%), Gaps = 10/136 (7%)

Query: 2 LFIDEVHRLPPEGQEKLFHFMDNGSWRRLGESADERSATVRLIFASTEDLEK-----HFL 56
LF+DE+ +P + Q +L + G + +G + VR++ A+ +DL++ F
Sbjct: 235 LFLDEIGDMPMDAQTRLLRVLQQGEYTTVG-GRTPIRSDVRIVAATNKDLKQSINQGLFR 293

Query: 57 ATFIRRIPVI-VKILPIAERGQFERLAFIHHFFRREAQRLNHD-LALDGEIVSQLMRETL 114
R+ V+ +++ P+ +R E + + F ++A++ D D E + +
Sbjct: 294 EDLYYRLNVVPLRLPPLRDRA--EDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPW 351

Query: 115 EGNVGGLENLIRNICA 130
GNV LENL+R + A
Sbjct: 352 PGNVRELENLVRRLTA 367


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13770HTHFIS260.047 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.047
Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 4/51 (7%)

Query: 89 QALCADRQDSLAQLIGAQGSLQEALRQCKAAISYPGAGLPLLLRGPTGTGK 139
L D QD + L+G ++QE R + L L++ G +GTGK
Sbjct: 127 SKLEDDSQDGMP-LVGRSAAMQEIYRVLARLMQTD---LTLMITGESGTGK 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13750CARBMTKINASE300.012 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 29.8 bits (67), Expect = 0.012
Identities = 18/87 (20%), Positives = 32/87 (36%), Gaps = 10/87 (11%)

Query: 199 AMAEHRGDPAWENKLARFFAASSEFEALWHQRYEVRGVENQIKHFNHPQLGRFSLQQMYW 258
A+ + ++E + + + + + YEV I H N PQ+G L
Sbjct: 13 ALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEV-----VITHGNGPQVGSLLLHMDAG 67

Query: 259 YSAPRNGSRLLVYLPMDEAGEQALAWL 285
+ + PMD AG + W+
Sbjct: 68 QATYGIPA-----QPMDVAGAMSQGWI 89


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13740TCRTETB1044e-26 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 104 bits (260), Expect = 4e-26
Identities = 77/409 (18%), Positives = 161/409 (39%), Gaps = 29/409 (7%)

Query: 21 MLPLIDTSITNVALDAITHTLAASATQLELIVALYGVAFAVCLAMGSKLGDNYGRRRLFM 80
+++ + NV+L I + + + + F++ A+ KL D G +RL +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 81 WGVALFGIASLLCGMANSIGALL-AARTLQGAGAALIVPQILATLHVTLKGPAH-ARAIS 138
+G+ + S++ + +S +LL AR +QGAGAA P ++ + + +A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAF-PALVMVVVARYIPKENRGKAFG 142

Query: 139 LYGGIGGIAFIVGQMGGGWLVSADIAGLGWRNAFFINVPICLLVLALSRRYVPETRRETP 198
L G I + VG GG + + W ++ + +P+ ++ + +
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHY----IHW--SYLLLIPMITIITVPFLMKLLKKEVRIK 196

Query: 199 SRIDWQGTLYL-ALILCCLLFPMALGPELHWPLWLQLMLVAVLPLLFAMRQSALRQQQRG 257
D +G + + I+ +LF + L++ + L+F ++ ++
Sbjct: 197 GHFDIKGIILMSVGIVFFMLFTTSY-------SISFLIVSVLSFLIF------VKHIRKV 243

Query: 258 DHPLLPPRLLQLTSIRFGMAIALLFFGAWSGFMFCMALTMQEGLGMAPWQSGNSFIALG- 316
P + P L + G+ + FG +GF+ + M++ ++ + G+ I G
Sbjct: 244 TDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGT 303

Query: 317 VAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCATFSRFGVATNALTLVPATALI 376
++ I L+ R +L G+ L F + T + + +
Sbjct: 304 MSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTAS-----FLLETTSWFMTIIIVFV 358

Query: 377 GYGQALIVNSFYRIGMRDISASDAGAGSAILSTLQQATLGLGPAILGSL 425
G + I + +AGAG ++L+ + G G AI+G L
Sbjct: 359 LGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGL 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13660PF00577672e-13 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 66.8 bits (163), Expect = 2e-13
Identities = 106/699 (15%), Positives = 207/699 (29%), Gaps = 99/699 (14%)

Query: 125 KDASLSLDTRSFYLELTVNRAAMQAAILPRTNMLGESTAQN--LSSVLNYSMGSYYNKYE 182
DA+ LD L LT+ +A M + + +LNY+ +
Sbjct: 143 HDATAQLDVGQQRLNLTIPQAFMS---NRARGYIPPELWDPGINAGLLNYNFSGNSVQNR 199

Query: 183 ---NTDNASSYLTL-DNT--WSLR-EHHLNFNGSLYGIGTGNQESKLYRSMYERDYQGRR 235
N+ A L N W LR ++N S G+ N+ + ERD R
Sbjct: 200 IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTW-LERDIIPLR 258

Query: 236 --LAMGMVDTWNLQSIASMSALNSSRIYGVSYGNKSSSQTQDNTLALVPVTVFLPA---- 289
L +G + G + D+ + F P
Sbjct: 259 SRLTLG-------DGYTQGDIFDGINFRGAQLAS-------DDNMLPDSQRGFAPVIHGI 304

Query: 290 ---AGEVHVYRDGKLLSIQNFSMGSYELDTSRLPFGIYNVDIQVVV---NGRVVSSRTAN 343
+V + ++G + G + ++ + + D+QV + +G
Sbjct: 305 ARGTAQVTIKQNGYDIYNSTVPPGPFTIND--IYAAGNSGDLQVTIKEADGSTQI----- 357

Query: 344 INKTFARKSSVT--GDLSWQTFGGSLEYNKMDYRHKY----NINYGTKNTWIAGIAAATS 397
++ + G + G + +G W +
Sbjct: 358 FTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA 417

Query: 398 QPWLS---GVNLKTTLYG---FDT--------NGVNETEANVIFNDAFSFNQQGLLATDG 443
+ + G+ G D + +V F S N+ G
Sbjct: 418 DRYRAFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLV 477

Query: 444 SWQ-STSTFNMSLPDGYG--NLWGSRQYSSIGNALPMQQNDYVTIGAN------ANLRKI 494
++ STS + + D + + + + DY + N + +
Sbjct: 478 GYRYSTSGY-FNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQ 536

Query: 495 APFLGTLSVSRTNNKYTGSTYTNVDYDQSLLAN-RYATVSLRAGIQNYQYNNHENLRDKY 553
TL +S ++ Y G++ + + L +L + N + RD+
Sbjct: 537 LGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSY---SLTKNAWQKGRDQM 593

Query: 554 VNIDVSIPFSTWLSTGVSSQNGNMLANATLRKSFDDSAITQVGAS--------VSKQIKQ 605
+ ++V+IPFS WL + SQ + A+ ++ + G +S ++
Sbjct: 594 LALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQT 653

Query: 606 NKNDDSRYRSDDYAANGYVSYDTKYNAGTVSVSRSSQHSSNYSLSSQGSLAWTEKNVYVG 665
S ++Y Y + S S G + V +G
Sbjct: 654 GYAGGGDGNSGST-GYATLNYRGGYGNANIGYSHSDDIKQ-LYYGVSGGVLAHANGVTLG 711

Query: 666 KGTQTAGLVVNTNFSGKGRMMAQINGQNYPLT---GKSNFISLPPYAEYKVELMNDKNSE 722
+ ++V G A++ Q T G + Y E +V L +
Sbjct: 712 QPLNDTVVLVKA----PGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL-DTNTLA 766

Query: 723 DSVDIVNGRRNKVVLYPGNVSVINPEIKQLVTVFGRVKD 761
D+VD+ N VV G + + + + + +
Sbjct: 767 DNVDLDNAVA-NVVPTRGAIVRAEFKARVGIKLLMTLTH 804


66KPHS_13540KPHS_13410Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_13540-2163.325372putative aldehyde dehydrogenase
KPHS_13530-2164.017605hypothetical protein
KPHS_13520-1133.744863hypothetical protein
KPHS_135100143.876795putative amino acid/amine transport protein
KPHS_135001123.148279hypothetical protein
KPHS_134900134.076635putative deacetylase
KPHS_134800153.811668transporter
KPHS_134700142.910285regulatory protein
KPHS_134601143.099365putative iron-sulfur modifier protein
KPHS_134501142.367930putative arylsulfatase
KPHS_134402132.575380putative 3-ketoacyl-(acyl-carrier-protein)
KPHS_134302110.771745molybdenum cofactor biosynthesis protein F
KPHS_134202120.953580putative aldo/keto reductase
KPHS_13410214-0.577098amino acid ABC transporter substrate-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13440DHBDHDRGNASE886e-23 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 88.2 bits (218), Expect = 6e-23
Identities = 62/253 (24%), Positives = 101/253 (39%), Gaps = 8/253 (3%)

Query: 3 RVVVITGGGTGIGAACPRLMRAAGDRVFITGRREAPLQAVADETGATA-----LVGDAAD 57
++ ITG GIG A R + + G + L+ V A A D D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 58 GEVWRQRLLPAILDQTGGIDVLICSAGGMGNSPAAETSDRQWREALDGNLTSAFASVRAC 117
+ + I + G ID+L+ AG + SD +W N T F + R+
Sbjct: 69 SAAIDE-ITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 118 LPSLIARR-GNVLFVASIASLAAGPQACGYVTAKHALIGLMRSVARDYGPQGVRANAVCP 176
++ RR G+++ V S + Y ++K A + + + + +R N V P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 177 GWVTTPMADEEMRPLMQAEGLSLTEAYQRVCRDVPLRRPASPEEIAQASQFLCSPQAAII 236
G T M + + + + +PL++ A P +IA A FL S QA I
Sbjct: 188 GSTETDM-QWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 237 SGATLVADGGASI 249
+ L DGGA++
Sbjct: 247 TMHNLCVDGGATL 259


67KPHS_13280KPHS_13080Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_13280-1224.172162hypothetical protein
KPHS_132700214.755741fructokinase
KPHS_132600204.098284sucrose porin
KPHS_132501184.125672sucrose-specific enzyme II
KPHS_132401172.845540beta-fructofuranosidase, sucrose-6-phosphate
KPHS_132302160.576822putative LacI family regulatory protein
KPHS_132202171.070592hypothetical protein
KPHS_132102171.441830hypothetical protein
KPHS_132002182.180112sugar ABC transport system permease component
KPHS_131901172.658583putative ABC transporter
KPHS_131801194.150613putative periplasmic binding protein
KPHS_131700195.022717hypothetical protein
KPHS_13160-2143.048277putative oxidoreductase
KPHS_13150-2142.518651putative oxidoreductase
KPHS_13140-118-0.215029gfo/Idh/MocA family oxidoreductase
KPHS_13130-120-1.462952hypothetical protein
KPHS_13120018-2.400902hypothetical protein
KPHS_13110019-2.556746lysyl-tRNA synthetase
KPHS_13100-116-2.391291di-/tripeptide transport protein
KPHS_13090116-4.408538lysine decarboxylase 1
KPHS_13080114-3.700798arginine/agmatine antiporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_1326056KDTSANTIGN300.022 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 30.3 bits (68), Expect = 0.022
Identities = 10/23 (43%), Positives = 13/23 (56%)

Query: 57 AEQKVQQLTQQQQQTQATTQQVA 79
+ + QQ QQQQ QAT Q+
Sbjct: 338 PQAQQQQGQGQQQQAQATAQEAV 360


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13130PF08280310.009 M protein trans-acting positive regulator
		>PF08280#M protein trans-acting positive regulator

Length = 530

Score = 30.6 bits (69), Expect = 0.009
Identities = 23/130 (17%), Positives = 45/130 (34%), Gaps = 21/130 (16%)

Query: 114 GETPLDEPISLSPPLSRVSLAAYCHKLNTFADLLLR------------DYDLQLAYHHHL 161
P+ E ++ L+ + L YC +LN F L + + Y + L
Sbjct: 57 SSLPITE-VAEKTGLTFLQLNHYCEELNAFFPDSLSMTIQKRMISCQFTHPSKETYLYQL 115

Query: 162 ----MMLVEHDDELERFLSHTHDNVGLAFDTGHAFVAGVEIPRVLHKYGHRIRHLHLKDV 217
+L L + + + L F++ R+ +R+ LK
Sbjct: 116 YASSNVL----QLLAFLIKNGSHSRPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLS 171

Query: 218 RPQVLGRLYR 227
+ +++G YR
Sbjct: 172 KNKIVGEEYR 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13100TCRTETA290.030 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 29.4 bits (66), Expect = 0.030
Identities = 41/208 (19%), Positives = 76/208 (36%), Gaps = 14/208 (6%)

Query: 44 SHAISLFSAYA-SLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGVESTSAWSL 102
+H L + YA P+LG +DR G R ++ + + ++ + W L
Sbjct: 43 AHYGILLALYALMQFACAPVLGAL-SDRF-GRRPVLLVSLAGAAVDYAIMAT-APFLWVL 99

Query: 103 YVALAIIICGY-GLFKSNISCLLGELYAHDDPRRDGGFSLLYAAGNVGSIAAPIACGLAA 161
Y + I+ G G + + ++ D+ R F + A G +A P+ GL
Sbjct: 100 Y--IGRIVAGITGATGAVAGAYIADITDGDE--RARHFGFMSACFGFGMVAGPVLGGLMG 155

Query: 162 QWYGWHIGFALAGIGMFIGLMIFLSGSRHFRHT-RGVDKPALRAVKFVLPTWGWLLVMLC 220
+ H F A + + FL+G + +G +P R L ++ W M
Sbjct: 156 G-FSPHAPFFAAA---ALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTV 211

Query: 221 LAPVFFTLLLQNNWSGYLLAIVCLFAAQ 248
+A + + A+ +F
Sbjct: 212 VAALMAVFFIMQLVGQVPAALWVIFGED 239


68KPHS_12980KPHS_11880Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_12980127-4.140834hypothetical protein
KPHS_12970131-5.065103putative citrate synthase
KPHS_12960235-6.612548hypothetical protein
KPHS_12950233-6.054437hypothetical protein
KPHS_12940333-5.977451hypothetical protein
KPHS_12930232-6.067752hypothetical protein
KPHS_12920132-6.494704hypothetical protein
KPHS_12910229-5.553028hypothetical protein
KPHS_12900233-6.690940hypothetical protein
KPHS_12890338-7.911456hypothetical protein
KPHS_12880539-8.353477antirepressor protein
KPHS_12870439-7.901745hypothetical protein
KPHS_12860438-7.979835hypothetical protein
KPHS_12850336-7.449226hypothetical protein
KPHS_12840231-5.363469transglycosylase
KPHS_12830127-4.463112hypothetical protein
KPHS_12820129-4.403146hypothetical protein
KPHS_12810129-4.642587hypothetical protein
KPHS_12800126-3.651238hypothetical protein
KPHS_12790125-3.165005hypothetical protein
KPHS_12780325-2.942140hypothetical protein
KPHS_12770326-2.404241hypothetical protein
KPHS_12760327-2.870714hypothetical protein
KPHS_12750231-8.837557hypothetical protein
KPHS_12740133-9.990340hypothetical protein
KPHS_12730141-11.390991hypothetical protein
KPHS_12720244-13.538219hypothetical protein
KPHS_12710143-12.636523transposase InsC for insertion sequence IS903
KPHS_12700138-11.353214transposase
KPHS_12690129-5.106627hypothetical protein
KPHS_12680229-4.305389hypothetical protein
KPHS_12670228-3.393972hypothetical protein
KPHS_12660228-2.911804putative phage head morphogenesis protein
KPHS_12650229-2.866001hypothetical protein
KPHS_12640228-2.702809hypothetical protein
KPHS_12630230-3.540002hypothetical protein
KPHS_12620331-3.759903hypothetical protein
KPHS_12610232-3.207406hypothetical protein
KPHS_12600131-3.524319hypothetical protein
KPHS_12590128-3.653522hypothetical protein
KPHS_12580326-3.432939hypothetical protein
KPHS_12570427-3.379756**hypothetical protein
KPHS_12560428-3.830937putative ninG protein
KPHS_12550631-4.690188hypothetical protein
KPHS_12540533-4.032895Eaa1
KPHS_12530634-4.789028RelE family toxin-antitoxin system
KPHS_12520534-5.198311hypothetical protein
KPHS_12510433-5.142796hypothetical protein
KPHS_12500431-5.008632hypothetical protein
KPHS_12490531-4.829857replicative DNA helicase
KPHS_12480429-6.649683hypothetical protein
KPHS_12470234-6.863650hypothetical protein
KPHS_12460236-6.452857bacteriophage CII family protein
KPHS_12450240-7.165928hypothetical protein
KPHS_12440236-7.206614regulatory protein CI bacteriophage origin
KPHS_12430338-7.037452hypothetical protein
KPHS_12420135-6.129620hypothetical protein
KPHS_12410035-4.038011hypothetical protein
KPHS_12400134-3.751042hypothetical protein
KPHS_12390234-3.563123hypothetical protein
KPHS_12380026-2.350223putative phage-like protein
KPHS_12370021-1.729765phage N-6-adenine-methyltransferase
KPHS_12360216-0.911733hypothetical protein
KPHS_12350415-1.710829phage/conjugal plasmid C-4 type zinc finger
KPHS_12340314-1.496233site-specific recombinase, phage integrase
KPHS_12330217-1.163647*bifunctional 5,10-methylene-tetrahydrofolate
KPHS_12320117-1.240944hypothetical protein
KPHS_123100170.203344cysteinyl-tRNA synthetase
KPHS_12300-1171.195863peptidyl-prolyl cis-trans isomerase B
KPHS_12290-1150.342744UDP-2,3-diacylglucosamine hydrolase
KPHS_12280015-0.076544hypothetical protein
KPHS_12270-1122.725413phosphoribosylaminoimidazole carboxylase
KPHS_12260-1123.069585phosphoribosylaminoimidazole carboxylase ATPase
KPHS_122500112.475165tRNA 2-selenouridine synthase
KPHS_122400112.184608putative outer membrane protein
KPHS_122304133.546640hypothetical protein
KPHS_122202152.996217putative oxidoreductase
KPHS_12200116-0.215347putative ABC transporter ATP-binding protein
KPHS_12210014-1.135339acyl-CoA thioesterase I
KPHS_12190015-0.516386short chain dehydrogenase/reductase family
KPHS_12180017-0.653549putative thioredoxin protein
KPHS_12170-1141.262091putative metal resistance protein
KPHS_12160-1132.477914putative ABC transporter ATP-binding protein
KPHS_12150-1133.289602putative protease
KPHS_12140-1152.411555hypothetical protein
KPHS_121300141.315674copper responsive transcription regulator
KPHS_121200131.111634copper exporting ATPase
KPHS_12110214-1.063658hypothetical protein
KPHS_12100113-0.736462hypothetical protein
KPHS_12090013-0.451677bifunctional UDP-sugar hydrolase/5'-nucleotidase
KPHS_120800140.445093fosmidomycin resistance protein
KPHS_12070-2130.761872hypothetical protein
KPHS_12060-2150.170689hypothetical protein
KPHS_12050-1150.948192putative cation:proton antiport protein
KPHS_12040-1200.298181inosine-guanosine kinase
KPHS_120302190.619437ferrochelatase
KPHS_120202171.639996adenylate kinase
KPHS_120102161.776227heat shock protein 90
KPHS_120001163.170661recombination protein RecR
KPHS_119904163.204161hypothetical protein
KPHS_119804183.126385DNA polymerase III subunits gamma and tau
KPHS_119703162.625170DNA polymerase III subunits gamma and tau
KPHS_119603130.006259adenine phosphoribosyltransferase
KPHS_11950312-0.730538hypothetical protein
KPHS_11940211-0.973153primosomal replication protein N''
KPHS_11930113-1.675474hypothetical protein
KPHS_11920112-2.034133hypothetical protein
KPHS_11910013-2.734383potassium efflux protein KefA
KPHS_11900117-3.846914DNA-binding transcriptional repressor AcrR
KPHS_11890117-3.520668acridine efflux pump
KPHS_11880216-3.917750acridine efflux pump
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12930PYOCINKILLER340.004 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 34.4 bits (78), Expect = 0.004
Identities = 29/129 (22%), Positives = 44/129 (34%), Gaps = 7/129 (5%)

Query: 10 AQFAAEAAVSAAEAKQYLIEVQQGYQDISATTQEAINAATAAEAAKSAAETAEQNSSTSA 69
AA+A++ AA A + +Q + +E A AA + A A N S A
Sbjct: 206 TLTAAKASIEAAAANK---AREQAAAEAKRKAEEQARQQAAIRAANTYAMPA--NGSVVA 260

Query: 70 AASSESATAAAGSAAQAEEYADNASDYAQNKFTFYKTASDPDGTIAGLAATTDGQSFWVA 129
A+ A AA + +A S A L ++ W
Sbjct: 261 TAAGRGLIQVAQGAASLAQAISDAIAVLGR--VLASAPSVMAVGFASLTYSSRTAEQWQD 318

Query: 130 QGPDALSAA 138
Q PD++ A
Sbjct: 319 QTPDSVRYA 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12910TCRTETB300.005 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 30.2 bits (68), Expect = 0.005
Identities = 17/53 (32%), Positives = 28/53 (52%), Gaps = 9/53 (16%)

Query: 17 SLRSLIDTFNASVAPEDWIDTFYDLVFNIETCGDYGLMCWGKIVD---VERLL 66
SL + + FN A +W++T + L F+I G +GK+ D ++RLL
Sbjct: 36 SLPDIANDFNKPPASTNWVNTAFMLTFSI------GTAVYGKLSDQLGIKRLL 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12800THERMOLYSIN280.020 Thermolysin metalloprotease (M4) family signature.
		>THERMOLYSIN#Thermolysin metalloprotease (M4) family signature.

Length = 544

Score = 27.7 bits (61), Expect = 0.020
Identities = 10/37 (27%), Positives = 19/37 (51%)

Query: 57 GFVYGDLPWTFHLAASSPSIKYIDNWQTTQMTTRSVL 93
G +G + W F +A S+ + + W+T + S+L
Sbjct: 11 GLAFGLMAWPFGASAKGKSMVWNEQWKTPSFVSGSLL 47


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12730CHLAMIDIAOM6290.010 Chlamydia cysteine-rich outer membrane protein 6 si...
		>CHLAMIDIAOM6#Chlamydia cysteine-rich outer membrane protein 6

signature.
Length = 547

Score = 28.9 bits (64), Expect = 0.010
Identities = 35/101 (34%), Positives = 47/101 (46%), Gaps = 14/101 (13%)

Query: 63 VPSGFVHRDGQASITIWLGQASMLIQPG--REITLMVAGDFWAKTSTAAT----RGQKVF 116
VP G+ H GQ +T LG +QPG R IT+ + + AT G K
Sbjct: 250 VPDGYAHSSGQRVLTFTLGD----MQPGEHRTITVEFCPLKRGRATNIATVSYCGGHKNT 305

Query: 117 ASLTT--GE--VQVAAAGATVAGFIETAFYAASDCDAGELV 153
AS+TT E VQV+ AGA + + Y S + G+LV
Sbjct: 306 ASVTTVINEPCVQVSIAGADWSYVCKPVEYVISVSNPGDLV 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12680BINARYTOXINA320.003 Clostridial binary toxin A signature.
		>BINARYTOXINA#Clostridial binary toxin A signature.

Length = 454

Score = 32.3 bits (73), Expect = 0.003
Identities = 18/70 (25%), Positives = 31/70 (44%)

Query: 2 VVSDPAFLSTSKEKKIAGMFSIGGVMLQIETNKGDKGLDVTGLSSNKHEDETLLPRNAKM 61
V++ P F+STS F+ ++L+I K G ++ + E E LL +K
Sbjct: 371 VITYPNFISTSIGSVNMSAFAKRKIILRINIPKDSPGAYLSAIPGYAGEYEVLLNHGSKF 430

Query: 62 EVIGVHPPKS 71
++ V K
Sbjct: 431 KINKVDSYKD 440


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12630HTHFIS270.029 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.7 bits (59), Expect = 0.029
Identities = 11/21 (52%), Positives = 15/21 (71%)

Query: 34 FLNEISDIPIANKAYRLRVLQ 54
FL+EI D+P+ + LRVLQ
Sbjct: 236 FLDEIGDMPMDAQTRLLRVLQ 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12580ARGDEIMINASE314e-04 Bacterial arginine deiminase signature.
		>ARGDEIMINASE#Bacterial arginine deiminase signature.

Length = 409

Score = 30.6 bits (69), Expect = 4e-04
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 6/52 (11%)

Query: 30 WFHGLDWNFIALASGVIIGVA-TYLTNLYFKRRWTKMYQ---QSLDRGYGGP 77
W G N +A+A G II + ++TN F+ K+++ L RG GGP
Sbjct: 348 WNDGA--NVLAIAPGEIIAYSRNHVTNKLFEENGIKVHRIPSSELSRGRGGP 397


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12240ECOLNEIPORIN290.025 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 29.4 bits (66), Expect = 0.025
Identities = 20/95 (21%), Positives = 34/95 (35%), Gaps = 14/95 (14%)

Query: 60 GDGDKGSYKRNG---FDGGTRFRFAADYYLFDDISWISYYELGVNIPALFDWDNHYAEGA 116
+G + + G D G++ F L + + I E +I A
Sbjct: 39 HNGAQAASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASI----------AGTD 88

Query: 117 NNTTRRMLYTGLKSDTWGTLTYGQQNSIYYDVVGV 151
+ R + GLK +G L G+ NS+ D +
Sbjct: 89 SGWGNRQSFIGLKGG-FGKLRVGRLNSVLKDTGDI 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12200PF05272280.025 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.025
Identities = 13/28 (46%), Positives = 16/28 (57%)

Query: 33 VKPRQTIALIGESGSGKSTLLAILAGLD 60
K ++ L G G GKSTL+ L GLD
Sbjct: 593 CKFDYSVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12190DHBDHDRGNASE792e-19 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 78.9 bits (194), Expect = 2e-19
Identities = 47/212 (22%), Positives = 79/212 (37%), Gaps = 7/212 (3%)

Query: 16 KTVLVTGCSSGIGLESALDLTRQGFRVLAA-CRKAEDVARMQELGLTG-----ILLDLDD 69
K +TG + GIG A L QG + A + + L D+ D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 70 PQSVERAAAEVIALTDNRLYGLFNNAGYGVYGPLNTISRQQMEQQFSANFFGAHQLTMLL 129
+++ A + + L N AG G ++++S ++ E FS N G + +
Sbjct: 69 SAAIDEITARIEREMG-PIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 130 LPAMTPHGEGRIVMTSSVMGLIASPGRGAYAASKYALEAWSDALRMELRHSGIQVSLIEP 189
M G IV S + AYA+SK A ++ L +EL I+ +++ P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 190 GPIRTRFTDNVNQTQSDKPVENPGIAARFTLG 221
G T ++ ++ G F G
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTG 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12080TCRTETA384e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 38.3 bits (89), Expect = 4e-05
Identities = 38/143 (26%), Positives = 54/143 (37%), Gaps = 5/143 (3%)

Query: 55 FSLTFVQIGMITLTFQLTSSLFQPVI-GYITDKRSMPWSLPVGMCFTLCGLILLALAGSF 113
F IG+ F + SL Q +I G + + +L +GM G ILLA A
Sbjct: 241 FHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRG 300

Query: 114 GMVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSLFQVGGNFGSSLGPLLAAVIIA 173
M L+ +G + ++R R G Q + S +GPLL I A
Sbjct: 301 WMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYA 360

Query: 174 ---PYGKGNVAWFVLAALLAIVV 193
G AW AAL + +
Sbjct: 361 ASITTWNG-WAWIAGAALYLLCL 382



Score = 32.1 bits (73), Expect = 0.004
Identities = 33/133 (24%), Positives = 51/133 (38%), Gaps = 2/133 (1%)

Query: 267 LHLFAFLFAVAAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLVLPYASLEWTGILTVIIG 326
L L+A + A + G + D+ GR+ V+ S+ G A ++ A W + I+
Sbjct: 49 LALYALMQFACAP--VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVA 106

Query: 327 FILASAFSAILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLLADHTSIDLVYKIC 386
I + + Y ++ G F FG G + VLG L S +
Sbjct: 107 GITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAA 166

Query: 387 AFLPLLGFLTIFL 399
A L L FLT
Sbjct: 167 AALNGLNFLTGCF 179


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_12010DNABINDINGHU300.005 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 30.4 bits (69), Expect = 0.005
Identities = 8/39 (20%), Positives = 22/39 (56%), Gaps = 2/39 (5%)

Query: 488 ESLDKLADEVDESTKEAEKALEPFVERVKNLL--GDRVK 524
+ + K+A+ + + K++ A++ V + L G++V+
Sbjct: 6 DLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQ 44


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11910GPOSANCHOR474e-07 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 46.6 bits (110), Expect = 4e-07
Identities = 42/282 (14%), Positives = 95/282 (33%), Gaps = 4/282 (1%)

Query: 31 RAADLPDRAEVQSQLNTLNKQKELTPQDKLVQQDLTQTLETLDKIERIKSETAQLRQQVE 90
+ +DL + N +EL+ + ++++ E KI+ +++ A L + +E
Sbjct: 72 KNSDLSFNNKALKDHND-ELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALE 130

Query: 91 QAPAKLRQAVESLNNLSDVPNDDATRKTLSTLSLRQLESRVTQTLDDLQNAQNDLATYNS 150
A + L A RK +L + T ++ + + A +
Sbjct: 131 GAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEA 190

Query: 151 QLVSLQTQPERVQNAMFNASQQLQQIRNRLNGTSVGD---ETLRPTQQVLLQAQQALLNA 207
+ L+ E N S +++ + + E A A +
Sbjct: 191 RQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKT 250

Query: 208 QIEQQRKSLEGNTILQDTLQKQRDYVTAWSNRLEHQLQLLQEAVNSKRLTLTEKTAQEAV 267
++ L+ L+ ++ TA S +++ K + A
Sbjct: 251 LEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNAN 310

Query: 268 TPDETARIQANPLVKQELDINHQLSEKLIQATENGNQLVQRN 309
+ A+ K++L+ HQ E+ + +E Q ++R+
Sbjct: 311 RQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRD 352


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11900HTHTETR1852e-61 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 185 bits (470), Expect = 2e-61
Identities = 170/213 (79%), Positives = 194/213 (91%)

Query: 1 MARKTKQQARETRQLILDVALRLFSQQGVSSTSLATIAKAAGVTRGAIYWHFKNKSDLFN 60
MARKTKQ+A+ETRQ ILDVALRLFSQQGVSSTSL IAKAAGVTRGAIYWHFK+KSDLF+
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EIWELSDASISDLEIEYRAKFPNDPLSVIREILVYVLEATVTEERRRLMMEIIYHKCEFV 120
EIWELS+++I +LE+EY+AKFP DPLSV+REIL++VLE+TVTEERRRL+MEII+HKCEFV
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 121 GEMTVVQQAQRQLSLASYERIEQTLKECIAAKLLPANLLTRRAAVLMRSYLSGLMENWLF 180
GEM VVQQAQR L L SY+RIEQTLK CI AK+LPA+L+TRRAA++MR Y+SGLMENWLF
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 181 APDSFDLHAEARDYVAILLEMYQFCPTLRGPES 213
AP SFDL EARDYVAILLEMY CPTLR P +
Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPAT 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11890RTXTOXIND424e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 4e-06
Identities = 29/210 (13%), Positives = 74/210 (35%), Gaps = 19/210 (9%)

Query: 100 TYQASYDSAKGDLAKAQAAANMDQLTVKRYQKLLGTKYISQQDYDTAVATA-QQSNAAVV 158
+ Y A +L ++ + + ++ Q + + +Q+ +
Sbjct: 256 EQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLV---TQLFKNEILDKLRQTTDNIG 312

Query: 159 AAKAAVETARINLAYTKVTSPISGRIGK-STVTEGALVQNGQTTALATVQQLDPIYVDVT 217
+ + + +P+S ++ + TEG +V +T + V + D + V
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETL-MVIVPEDDTLEVTAL 371

Query: 218 QSSNDFLRLKQEL-ADGRLKQENGK------AKVELVTNDGLKYPQSGTLEFSDVTVDQT 270
+ D + A +++ KV+ + D ++ + G + +++++
Sbjct: 372 VQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEEN 431

Query: 271 TGSITLRAIFPNPDHTLLPGMFVRARLEEG 300
S + I L GM V A ++ G
Sbjct: 432 CLSTGNKNIP------LSSGMAVTAEIKTG 455



Score = 29.0 bits (65), Expect = 0.039
Identities = 17/78 (21%), Positives = 33/78 (42%), Gaps = 3/78 (3%)

Query: 48 APLQITTELPGR-TSAYRIAEVRPQVSGIILKRNFV-EGSDIQAGVSLYQIDPATYQASY 105
++I G+ T + R E++P + I+ K V EG ++ G L ++ +A
Sbjct: 78 GQVEIVATANGKLTHSGRSKEIKPIENSIV-KEIIVKEGESVRKGDVLLKLTALGAEADT 136

Query: 106 DSAKGDLAKAQAAANMDQ 123
+ L +A+ Q
Sbjct: 137 LKTQSSLLQARLEQTRYQ 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11880ACRIFLAVINRP13650.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1365 bits (3535), Expect = 0.0
Identities = 805/1032 (78%), Positives = 911/1032 (88%)

Query: 1 MPNFFIDRPIFAWVIAIIIMLAGGLSILKLPVAQYPTIAPPAISITAMYPGADAETVQNT 60
M NFFI RPIFAWV+AII+M+AG L+IL+LPVAQYPTIAPPA+S++A YPGADA+TVQ+T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDHLMYMSSNGDSTGTATITLTFESGTDPDIAQVQVQNKLALATPLLPQ 120
VTQVIEQNMNGID+LMYMSS DS G+ TITLTF+SGTDPDIAQVQVQNKL LATPLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGISVEKASSSFLMVVGVINTNGTMNQDDISDYVAANMKDPISRTSGVGDVQLFGS 180
EVQQQGISVEK+SSS+LMV G ++ N QDDISDYVA+N+KD +SR +GVGDVQLFG+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWMDPNKLNNFQLTPVDVISALKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRL 240
QYAMRIW+D + LN ++LTPVDVI+ LK QN Q+AAGQLGGTP + GQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 TNTEEFGNILLKVNQDGSQVRLRDVAKIELGGESYDVVAKFNGQPASGLGIKLATGANAL 300
N EEFG + L+VN DGS VRL+DVA++ELGGE+Y+V+A+ NG+PA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTANAIRAELAKMEPFFPSGMKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ 360
DTA AI+A+LA+++PFFP GMK++YPYDTTPFV++SIHEVVKTL EAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
N RATLIPTIAVPVVLLGTFA+LAAFG+SINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 AEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480
E+ LPPKEAT KSM QIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATMLKPIQKGSHGATTGFFGWFNRMFDKSTHHYTDSVGNILRSTGRY 540
SVLVALILTPALCAT+LKP+ H GFFGWFN FD S +HYT+SVG IL STGRY
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 541 LVLYLIIVVGMAWLFVRLPSSFLPDEDQGVFLSMAQLPAGATQERTQKVLDEMTNYYLTK 600
L++Y +IV GM LF+RLPSSFLP+EDQGVFL+M QLPAGATQERTQKVLD++T+YYL
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 601 EKDNVESVFAVNGFGFAGRGQNTGIAFVSLKDWSQRPGEENKVEAITARAMGYFSQIKDA 660
EK NVESVF VNGF F+G+ QN G+AFVSLK W +R G+EN EA+ RA +I+D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 661 MVFAFNLPAIVELGTATGFDFELIDQGGLGHEKLTQARNQLFGMVAQHPDVLTGVRPNGL 720
V FN+PAIVELGTATGFDFELIDQ GLGH+ LTQARNQL GM AQHP L VRPNGL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 721 EDTPQFKIDIDQEKAQALGVSISDINTTLGAAWGGSYVNDFIDRGRVKKVYIMSEAKYRM 780
EDT QFK+++DQEKAQALGVS+SDIN T+ A GG+YVNDFIDRGRVKK+Y+ ++AK+RM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 781 LPEDIGKWYVRGSDGQMVPFSAFSTSRWEYGSPRLERYNGLPSLEILGQAAPGKSTGEAM 840
LPED+ K YVR ++G+MVPFSAF+TS W YGSPRLERYNGLPS+EI G+AAPG S+G+AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 841 ALMEELAGKLPSGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFSV 900
ALME LA KLP+GIGYDWTGMSYQERLSGNQAPAL AIS +VVFLCLAALYESWSIP SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 901 MLVVPLGVVGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGLI 960
MLVVPLG+VG LLAAT NDVYF VGLLTTIGLSAKNAILIVEFAKDLMEKEGKG++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 961 EATLEAVRMRLRPILMTSLAFILGVMPLVISSGAGSGAQNAVGTGVMGGMVTATILAIFF 1020
EATL AVRMRLRPILMTSLAFILGV+PL IS+GAGSGAQNAVG GVMGGMV+AT+LAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1021 VPVFFVVVRRRF 1032
VPVFFVV+RR F
Sbjct: 1021 VPVFFVVIRRCF 1032


69KPHS_11550KPHS_11240Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_115502131.701101nitrogen regulatory protein P-II 2
KPHS_115403112.521866putative multidrug transporter
KPHS_115302101.701827ATP-binding component of a transport system
KPHS_115201100.771009putative LRP-like transcriptional regulator
KPHS_115101111.136918putative cysteine synthase/cystathionine
KPHS_115002110.347716cof family hydrolase
KPHS_114902110.085330ABC transporter substrate-binding protein
KPHS_11480116-1.971661queuosine biosynthesis protein QueC
KPHS_11470118-2.310056hypothetical protein
KPHS_11460218-2.159368hypothetical protein
KPHS_11450120-2.331334peptidyl-prolyl cis-trans isomerase
KPHS_11440526-3.307091transcriptional regulator HU subunit beta
KPHS_11420524-3.141766DNA-binding ATP-dependent protease La
KPHS_11430525-3.751307hypothetical protein
KPHS_11410525-3.345189ATP-dependent protease ATP-binding subunit
KPHS_11400320-1.838065ATP-dependent Clp protease proteolytic subunit
KPHS_11390321-2.799328trigger factor
KPHS_11380-120-2.909856hypothetical protein
KPHS_11370122-2.891487transcriptional regulator BolA
KPHS_11360223-2.990564hypothetical protein
KPHS_11350222-2.989502beta-lactamase synthesis regulator/muropeptide
KPHS_11340428-4.598967cytochrome o ubiquinol oxidase subunit II
KPHS_11330322-3.208280cytochrome o ubiquinol oxidase subunit I
KPHS_11320113-0.558156cytochrome o ubiquinol oxidase subunit III
KPHS_11310-1132.273436cytochrome o ubiquinol oxidase subunit IV
KPHS_11300-2132.783470protoheme IX farnesyltransferase
KPHS_11290-1145.234896hypothetical protein
KPHS_11280-2146.064966regulatory protein, IclR
KPHS_11270-2145.825174shikimate/quinate 5-dehydrogenase
KPHS_11260-2145.200207putative electron transfer
KPHS_11250-2154.313298NIPSNAP protein
KPHS_11240-2143.884872hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11540ACRIFLAVINRP310.018 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 31.0 bits (70), Expect = 0.018
Identities = 13/65 (20%), Positives = 27/65 (41%), Gaps = 1/65 (1%)

Query: 120 PVGQLISRVTNDTEVIRDLYVTVVATVLRSAALIGAMLVAMFSLDWRMALVAIAIFPAVL 179
P G + + T ++ VV T+ + L+ +++ +F + R L+ P VL
Sbjct: 318 PQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLV-FLVMYLFLQNMRATLIPTIAVPVVL 376

Query: 180 IVMII 184
+
Sbjct: 377 LGTFA 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11500HTHFIS290.024 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 28.6 bits (64), Expect = 0.024
Identities = 11/64 (17%), Positives = 23/64 (35%), Gaps = 10/64 (15%)

Query: 193 LAVLSQHLGFTLQECMAFGDAMNDREMLGSVGRGFIMGN----------AMPQLKAELPH 242
VL+Q L + +A + + ++ + +P++K P
Sbjct: 16 RTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRIKKARPD 75

Query: 243 LPVI 246
LPV+
Sbjct: 76 LPVL 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11440DNABINDINGHU1164e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 116 bits (293), Expect = 4e-38
Identities = 49/88 (55%), Positives = 65/88 (73%)

Query: 2 NKSQLIDKIAAGADISKAAAGRALDALIASVTESLQAGDDVALVGFGTFAVKERAARTGR 61
NK LI K+A +++K + A+DA+ ++V+ L G+ V L+GFG F V+ERAAR GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEITIAAAKVPGFRAGKALKDAV 89
NPQTG+EI I A+KVP F+AGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11420GPOSANCHOR330.004 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 33.1 bits (75), Expect = 0.004
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 12/144 (8%)

Query: 137 KQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQK 196
++ + L A QV R +++ ++ S+ +Q++A +
Sbjct: 277 TADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAK---KQLEAEHQ 333

Query: 197 ELGEMDDAPD-ENEALKRKIDAAKMPKEAKEKTEAELQKLKMMSPMS-AEATVVRGYIDW 254
+L E + + ++L+R +DA++ EAK++ EAE QKL+ + +S A +R +D
Sbjct: 334 KLEEQNKISEASRQSLRRDLDASR---EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDA 390

Query: 255 MVQVPWNARSKVKKDLRQAQEILD 278
+ A+ +V+K L +A L
Sbjct: 391 SRE----AKKQVEKALEEANSKLA 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11350TCRTETB385e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 38.3 bits (89), Expect = 5e-05
Identities = 42/196 (21%), Positives = 75/196 (38%), Gaps = 15/196 (7%)

Query: 221 RNNAWLI-LLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGMVNKTLGLFATILGALYG 279
R+N LI L ++ + + + ++++ + VN L +I A+YG
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 280 GVLMQRLTLFRALLIFGLLQGVSNAGYWLLSITDKHLYSMATAVFFENLCGGMGTAAFVA 339
L +L + R LL ++ S+ +S + + G G AAF A
Sbjct: 71 K-LSDQLGIKRLLLFGIIINC-------FGSVIGFVGHSFFSLLIMARFIQGAGAAAFPA 122

Query: 340 LLM----TLCNKSFSATQFALLSALSAVGRVYVGP-IAGWFVEAHGWSTFYLFSVVAAVP 394
L+M K F L+ ++ A+G VGP I G WS L ++ +
Sbjct: 123 LVMVVVARYIPKENRGKAFGLIGSIVAMG-EGVGPAIGGMIAHYIHWSYLLLIPMITIIT 181

Query: 395 GIALLLLCRQTLEHTQ 410
L+ L ++ +
Sbjct: 182 VPFLMKLLKKEVRIKG 197


70KPHS_11040KPHS_10920Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_11040218-0.309344hypothetical protein
KPHS_11030221-0.965938nucleoside channel, receptor of phage T6 and
KPHS_11020218-0.836587DeoR family transcriptional regulator
KPHS_11010219-0.788506hypothetical protein
KPHS_11000219-1.119385preprotein translocase subunit SecF
KPHS_10990217-0.696689preprotein translocase subunit SecD
KPHS_10980115-1.240303preprotein translocase auxillary subunit
KPHS_10970114-1.739940queuine tRNA-ribosyltransferase
KPHS_10960013-2.372707S-adenosylmethionine--tRNA
KPHS_10950321-5.499682hypothetical protein
KPHS_10940218-4.391034phosphodiesterase
KPHS_10930320-6.378143putative thiol-alkyl hydroperoxide reductase
KPHS_10920221-5.664827hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11030CHANNELTSX5370.0 Nucleoside-specific channel-forming protein Tsx signa...
		>CHANNELTSX#Nucleoside-specific channel-forming protein Tsx

signature.
Length = 294

Score = 537 bits (1384), Expect = 0.0
Identities = 294/294 (100%), Positives = 294/294 (100%)

Query: 1 MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60
MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE
Sbjct: 1 MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60

Query: 61 YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP 120
YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP
Sbjct: 61 YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP 120

Query: 121 FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN 180
FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN
Sbjct: 121 FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN 180

Query: 181 EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH 240
EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH
Sbjct: 181 EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH 240

Query: 241 ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF 294
ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF
Sbjct: 241 ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11020ARGREPRESSOR336e-04 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 32.5 bits (74), Expect = 6e-04
Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 7/66 (10%)

Query: 3 RRADRLFQIVQILRGRRLTT-----AALLAERLGVSERTVYRDIRDLSLSGVPVEGEAGS 57
+ R +I +I+ + T L + V++ TV RDI++L L V V GS
Sbjct: 2 NKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKELHL--VKVPTNNGS 59

Query: 58 GYRLLA 63
L
Sbjct: 60 YKYSLP 65


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11000SECFTRNLCASE342e-120 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 342 bits (880), Expect = e-120
Identities = 101/308 (32%), Positives = 172/308 (55%), Gaps = 12/308 (3%)

Query: 18 DFMRWDYWAFGISGFLLIVSIAIIGVRGFNWGLDFTGGTVIEITLEKPVDLDQMRDSLQK 77
DF RW + FG + ++I S+ + V G N+G+DF GGT I +D+ R +L+
Sbjct: 15 DFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAALEP 74

Query: 78 AGFEEPQVQNFGSSR------DIMVRMPPVHDANGSQELGSKVVTVINE------STSQN 125
+ + M+R+ D G++ G++ ++N+ +
Sbjct: 75 LELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAVDPA 134

Query: 126 AAVKRIEFVGPSVGADLAQTGALALIAALVCILIYVGFRFEWRLAAGVVIALAHDVVITM 185
+ E VGP V +L T +L+AA V I+ Y+ RFEW+ A G V+AL HDV++T+
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 186 GVLSLFHIEIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRGTPYEIFNVSLTQTL 245
G+ ++ ++ DLT VA+L+++ GYS+ND++VV DR+REN K + ++ N+S+ +TL
Sbjct: 195 GLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNETL 254

Query: 246 HRTLITSGTTLMVILMLFLFGGPILEGFSLTMLIGVSIGTASSIYVASALALKLGMKREH 305
RT++T TTL+ ++ + ++GG ++ GF M+ GV GT SS+YVA + L +G+ R
Sbjct: 255 SRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIVLFIGLDRNK 314

Query: 306 LIQQKVEK 313
+ +K
Sbjct: 315 EKKDPSDK 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10990SECFTRNLCASE695e-15 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 69.5 bits (170), Expect = 5e-15
Identities = 37/183 (20%), Positives = 88/183 (48%), Gaps = 5/183 (2%)

Query: 422 IQIVEERTIGPTLGMQNIKQGLEACLAGLVVSILFMIL-FYKKFGLIATSALIANLILIV 480
++I ++GP + + + + + LA VV + ++ + F +F L A AL+ +++L V
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 481 GIMSLIPGATLTMPGIAGIVLTLAVAVDANVLINERIKEEL--SNGRTVQQAIDEGYRGA 538
G+ +++ + +A ++ +++ V++ +R++E L ++ ++
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 539 FSSIFDANVTTLIKVIILYAVGTGAIKGFAITTGIGIATSMFTAIVGTRAIVNLLYGGKR 598
S +TTL+ ++ + G I+GF G+ T ++++ + IV L G R
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIV-LFIGLDR 312

Query: 599 VKK 601
K+
Sbjct: 313 NKE 315


71KPHS_10630KPHS_10340Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_106302151.401368putative permease
KPHS_106200140.829169putative inner membrane protein
KPHS_10610-112-0.026365D-alanyl-alanine synthetase A
KPHS_10600-2130.803485hypothetical protein
KPHS_10590-2130.893318hypothetical protein
KPHS_10580-3121.402645hypothetical protein
KPHS_10570-2131.450641transporter
KPHS_10560-2132.758999isochorismatase family protein
KPHS_10550-2143.067580beta-lactamase/D-alanine carboxypeptidase
KPHS_10540-3132.955665delta-aminolevulinic acid dehydratase
KPHS_10530-1164.289449acyltransferase
KPHS_10520-1184.314318taurine dioxygenase
KPHS_105100194.892941taurine transport system permease component
KPHS_105000184.916866taurine transporter ATP-binding subunit
KPHS_10490-1174.825862taurine ABC transporter substrate-binding
KPHS_104800185.391396homocysteine methyltransferase
KPHS_10470-1164.074778S-methylmethionine transporter
KPHS_10460-1183.689174hypothetical protein
KPHS_10450-1193.153708putative amino acid/amine transport protein
KPHS_10440-1192.313328ethanolamine ammonia-lyase, heavy chain
KPHS_104300211.711268ethanolamine ammonia-lyase small subunit
KPHS_104200221.046290hypothetical protein
KPHS_10410-1231.585312high-affinity branched-chain amino acid ABC
KPHS_10400-1232.039418high-affinity branched-chain amino acid ABC
KPHS_10390-1232.812024high-affinity branched-chain amino acid
KPHS_10380-1213.650800ATP-binding component of high-affinity
KPHS_10370-1214.725777ATP-binding component of leucine transport
KPHS_103600214.803496hypothetical protein
KPHS_10350-2203.202552cysteine transport system periplasmic binding
KPHS_10340-1193.313830glutamate/aspartate transport system ATP-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10630TCRTETA1268e-35 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 126 bits (318), Expect = 8e-35
Identities = 88/384 (22%), Positives = 157/384 (40%), Gaps = 15/384 (3%)

Query: 8 LISVWFGCFFTGLAISQILPFLPLYVSQLGVTSHEALSMWSGLTFSVTFLVSAIVSPMWG 67
LI + + I I+P LP + L ++ G+ ++ L+ +P+ G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY--GILLALYALMQFACAPVLG 64

Query: 68 SLADRKGRKLMLLRASLGMAIAILLQAFATNVWQLFILRAIMGLTSGYIPNAMALVASQV 127
+L+DR GR+ +LL + G A+ + A A +W L+I R + G+T A A +A
Sbjct: 65 ALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADIT 124

Query: 128 PRERSGWALSTLSTAQISGVIGGPLLGGFLADHVGLRMVFFITAILLTISFLVTLFLIKE 187
+ +S G++ GP+LGG + FF A L ++FL FL+ E
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 188 GVRPQVSKSERLTGKQVFASLPYPGL---VISLFFTTLVIQLCNGSIGPILALF-IKSMA 243
+ + + R AS + V +L ++QL + +F
Sbjct: 184 SHKGE-RRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFH 242

Query: 244 PDSNNIAFLAGMIAAVPGVSALISAPRLGKLGDRIGTSRILLATLCCAVVMFFAMSFVTT 303
D+ I +AA + +L A G + R+G R L+ + + ++F T
Sbjct: 243 WDATTIGI---SLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATR 299

Query: 304 PLQLGTLRFLLGFADGAMLPAVQTLLLKYSSDSVTGRIFGYNQSFMYLGNVAGPLIGA-- 361
+ LL G +PA+Q +L + + G++ G + L ++ GPL+
Sbjct: 300 GWMAFPIMVLLASG-GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAI 358

Query: 362 -SVSAMAGFRWVFIATAIIVFINL 384
+ S W +IA A + + L
Sbjct: 359 YAASITTWNGWAWIAGAALYLLCL 382


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10610SSPANPROTEIN310.006 Salmonella invasion protein InvJ signature.
		>SSPANPROTEIN#Salmonella invasion protein InvJ signature.

Length = 336

Score = 31.2 bits (70), Expect = 0.006
Identities = 18/56 (32%), Positives = 32/56 (57%), Gaps = 5/56 (8%)

Query: 73 QDPARIALRPSTVT-LAQIPGREAQQLINAESGQPLAAIDVIFPIVHGTLGEDGSL 127
+D +++ L+P+T+ L+Q+ G + + + A+S + IFP G GED SL
Sbjct: 212 KDVSQLPLQPTTIADLSQLTGGDEKMPLAAQSKPMMT----IFPTADGVKGEDSSL 263


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10560ISCHRISMTASE300.005 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 29.6 bits (66), Expect = 0.005
Identities = 15/53 (28%), Positives = 25/53 (47%)

Query: 44 GLEAGSEGFALLPELEQPAGALYVTKTACDAFYHTSLAQVLDEHDIQQFVICG 96
GL +G ++ EL L +TK AF T+L +++ + Q +I G
Sbjct: 98 GLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQLIITG 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10540BINARYTOXINB310.008 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 30.8 bits (69), Expect = 0.008
Identities = 18/69 (26%), Positives = 29/69 (42%)

Query: 254 DILRDIRERSDLPLGAYQVSGEYAMIKFAAQAGAIDEEKVVLESLGAIKRAGADLIFSYF 313
+ ++ + L L QV G A F +D E L I+ A +IF+
Sbjct: 466 NQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGK 525

Query: 314 ALDLAEKKI 322
L+L E++I
Sbjct: 526 DLNLVERRI 534


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10510SALSPVBPROT300.014 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein

signature.
Length = 591

Score = 29.7 bits (66), Expect = 0.014
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 4/38 (10%)

Query: 39 WAVAALQLISPLFLPPPGQVLQKLITIAGPQGFMDATL 76
++ A L LI+P FLP G+ L + +GP G TL
Sbjct: 7 FSSATLALITPPFLPKGGKALSQ----SGPDGLASITL 40


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10400FERRIBNDNGPP290.018 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 29.1 bits (65), Expect = 0.018
Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 2/57 (3%)

Query: 159 LWLLYRTRY--GMAIRAVAFDVNTVRLMGIDANRIISLVFALGSSLAALGGVFYSIS 213
L L+ R R MA+ + + +NT ID NRI++L + L ALG V Y ++
Sbjct: 4 LPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVA 60


72KPHS_10240KPHS_10080Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_102401183.640882phosphonate ABC superfamily ATP binding cassette
KPHS_102302204.157501putative inner membrane permease of ABC
KPHS_10220-1182.266540phosphonate ABC superfamily ATP binding cassette
KPHS_10210-2170.453721putative LysR-family transcriptional regulator
KPHS_10200-2151.120396family IB HAD-hydrolase
KPHS_10190-3171.234033hypothetical protein
KPHS_10180-115-2.813091lipoprotein
KPHS_10170-121-6.201907outer membrane pore protein E
KPHS_10160-129-8.346011hypothetical protein
KPHS_10150-127-7.204105gamma-glutamyl kinase
KPHS_10140-134-9.506983gamma-glutamyl phosphate reductase
KPHS_10130040-11.000276*putative site specific integrase
KPHS_10120138-10.720607cobyrinic acid a,c-diamide synthase
KPHS_10110026-4.477026hypothetical protein
KPHS_10100-1230.781029phage polarity suppression protein
KPHS_100903261.344127phage transcriptional activator, Ogr/delta
KPHS_100802271.834448hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10170ECOLIPORIN5360.0 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 536 bits (1381), Expect = 0.0
Identities = 225/384 (58%), Positives = 263/384 (68%), Gaps = 35/384 (9%)

Query: 1 MKKSTLALMMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFG 60
MK+ LAL++ +A+ A AAE+YNK+ NKLD+YGK+ +HYFSD SKDGDQTY+R G
Sbjct: 1 MKRKVLALVIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDDSSKDGDQTYMRVG 60

Query: 61 IKGETQINDDLTGYGRWESEFSGNKTESDSSQ-KTRLAFAGVKLKNYGSFDYGRNLGALY 119
KGETQIND LTGYG+WE N TE + + TRLAFAG+K +YGSFDYGRN G LY
Sbjct: 61 FKGETQINDQLTGYGQWEYNVQANTTEGEGANSWTRLAFAGLKFGDYGSFDYGRNYGVLY 120

Query: 120 DVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLTLQYQGKNE--- 176
DVE WTDM PEFGGDS DN+MT RA+G+ATYRNTDFFGLVDGL+ LQYQGKNE
Sbjct: 121 DVEGWTDMLPEFGGDSYTYADNYMTGRANGVATYRNTDFFGLVDGLNFALQYQGKNESQS 180

Query: 177 -------------GREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLAR-- 221
G + + NGDG G S +YD G F+ AAYT+SDRTN+Q
Sbjct: 181 ADDVNIGTNNRNNGDDIRYDNGDGFGISTTYDI-GMGFSAGAAYTTSDRTNEQVNAGGTI 239

Query: 222 GQGSKAEAWATGLKYDANNIYLATMYSETRKMTP-------ISGGFANKAQNFEAVAQYQ 274
G KA+AW GLKYDANNIYLATMYSETR MTP GG ANK QNFE AQYQ
Sbjct: 240 AGGDKADAWTAGLKYDANNIYLATMYSETRNMTPYGKTDKGYDGGVANKTQNFEVTAQYQ 299

Query: 275 FDFGLRPSLGYVLSKGKDIE----GVGSEDLVNYIDVGLTYYFNKNMNAFVDYKINQLKS 330
FDFGLRP++ +++SKGKD+ +DLV Y DVG TYYFNKN + +VDYKIN L
Sbjct: 300 FDFGLRPAVSFLMSKGKDLTYNNVNGDDKDLVKYADVGATYYFNKNFSTYVDYKINLLDD 359

Query: 331 DNKL----GINDDDIVALGMTYQF 350
D+ GI+ DDIVALGM YQF
Sbjct: 360 DDPFYKDAGISTDDIVALGMVYQF 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10150CARBMTKINASE384e-05 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 37.9 bits (88), Expect = 4e-05
Identities = 31/140 (22%), Positives = 53/140 (37%), Gaps = 20/140 (14%)

Query: 119 DTLRALLDNSI---------VPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLT 169
+T++ L++ + VPVI E+ + E V D D A AD ++LT
Sbjct: 177 ETIKKLVERGVIVIASGGGGVPVILEDGEIKGVE-AVIDKDLAGEKLAEEVNADIFMILT 235

Query: 170 DQPGLFTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMGTKLQAA-DVACRAG 228
D G + + +++V +++ + G MG K+ AA G
Sbjct: 236 DVNGAALY--YGTEKEQWLREV-KVEELRKYYEEG---HFKAGSMGPKVLAAIRFIEWGG 289

Query: 229 IDTIIAAGNRPDVIGHAMAG 248
IIA + A+ G
Sbjct: 290 ERAIIAH---LEKAVEALEG 306



Score = 29.0 bits (65), Expect = 0.032
Identities = 16/76 (21%), Positives = 33/76 (43%), Gaps = 13/76 (17%)

Query: 4 SQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQ----LHAMGHRIVIVTSG-------- 51
+ +V+ LG + L ++ + +++ VR+ A+ + A G+ +VI
Sbjct: 2 GKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSLL 61

Query: 52 -AIAAGREHLGYPELP 66
+ AG+ G P P
Sbjct: 62 LHMDAGQATYGIPAQP 77


73KPHS_09960KPHS_09860Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_09960-120-3.182724dihydrolipoamide acetyltransferase
KPHS_09950020-5.101882dihydrolipoamide dehydrogenase
KPHS_09940122-6.973745hypothetical protein
KPHS_09930123-7.599999putative phospho-2-dehydro-3-deoxyheptonate
KPHS_09920121-6.659627transcriptional antiterminator of glycerol
KPHS_09910017-4.506453putative L-xylulose kinase
KPHS_09900-117-3.7917233-oxoacyl-ACP reductase
KPHS_09890013-2.752636putative oxidoreductase protein
KPHS_09880-112-0.299864hypothetical protein
KPHS_09870-1182.923845glutathione S-transferase family protein
KPHS_098600213.470669KpLE2 phage-like element, putative
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_09960RTXTOXIND310.009 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.009
Identities = 14/56 (25%), Positives = 29/56 (51%), Gaps = 4/56 (7%)

Query: 44 SKIVNVLEAPFAGTLRRILAREGETLQVGAVLALAADASVSDAELDEFVARLATAK 99
SK + +E ++ I+ +EGE+++ G VL A ++A+ + + L A+
Sbjct: 96 SKEIKPIEN---SIVKEIIVKEGESVRKGDVLLK-LTALGAEADTLKTQSSLLQAR 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_09900DHBDHDRGNASE1022e-28 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 102 bits (255), Expect = 2e-28
Identities = 73/253 (28%), Positives = 124/253 (49%), Gaps = 16/253 (6%)

Query: 8 RTAIVTGGATGLGREFVLSLAKEGVNIC-FTYMREEEHPERLIETVKTSANVEIIAVKTD 66
+ A +TG A G+G +LA +G +I Y E+ E+++ ++K A A D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKL--EKVVSSLKAEARHAE-AFPAD 65

Query: 67 LSDEQSRENLFATCIDRLGKADILVNNAGIWLSGYVTEISPQDWDLVMNVNLKAIFHLSQ 126
+ D + + + A +G DILVN AG+ G + +S ++W+ +VN +F+ S+
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 127 LFVNHCLQHDQMGSILNITSQAAFHGSTTGHAHYAASKAGLVAFAISLAREVAKQKINVN 186
+ + + GSI+ + S A T A YA+SKA V F L E+A+ I N
Sbjct: 126 SVSKY-MMDRRSGSIVTVGSNPA-GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 187 NIAVGIMDTAMIRKN-IEQNPDSYVSR---------IPVGRVAQPQEIADIGVFMVSPKT 236
++ G +T M ++N V + IP+ ++A+P +IAD +F+VS +
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 237 SYMTGATLDVTGG 249
++T L V GG
Sbjct: 244 GHITMHNLCVDGG 256


74KPHS_09590KPHS_09440Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_09590019-4.436028neutral amino-acid efflux protein
KPHS_09580017-4.063835LysR family transcriptional regulator
KPHS_09570019-4.006063*DNA polymerase III
KPHS_09560120-3.276845RNase HI
KPHS_09550018-2.365309putative methyltransferase
KPHS_09540-117-2.097965hydroxyacylglutathione hydrolase
KPHS_09530-113-0.453682membrane-bound lytic murein transglycosylase D
KPHS_09520-212-0.622476UbiE/COQ5 family methyltransferase
KPHS_09510-212-1.158678endonuclease/exonuclease/phosphatase
KPHS_09500-215-1.396100putative drug efflux protein
KPHS_09490-216-2.847468LysR family transcriptional regulator
KPHS_09480-220-3.1485632,5-diketo-D-gluconate reductase B
KPHS_09470-118-2.658354***D,D-heptose 1,7-bisphosphate phosphatase
KPHS_09460017-1.728165DL-methionine transporter ATP-binding subunit
KPHS_09450115-1.189349DL-methionine transporter permease
KPHS_09440213-1.109163DL-methionine transporter substrate-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_09500TCRTETA638e-13 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 62.5 bits (152), Expect = 8e-13
Identities = 79/388 (20%), Positives = 145/388 (37%), Gaps = 26/388 (6%)

Query: 3 LALFALTIGAFAIGTTEFVIVGLVPTIAQQLSISLPSA---GLLVSIYALGVAIGAPVLT 59
+ L + + A IG +I+ ++P + + L S G+L+++YAL APVL
Sbjct: 9 VILSTVALDAVGIG----LIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLG 64

Query: 60 ALTGRMPRKQLLLALMVLFTAGNVLAWQAPGYETLILARLLTGLAHGVFFSIGSTIATSL 119
AL+ R R+ +LL + + AP L + R++ G+ G+ IA
Sbjct: 65 ALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADIT 124

Query: 120 VAKEKAASAIAIMFGGLTVALVTGVPFGTFIGQHFGWRETFLAVSILGVIALISSLILVP 179
E+A M +V G G +G F F A + L + ++ L+P
Sbjct: 125 DGDERAR-HFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLP 182

Query: 180 NNIPGRASASLRDQMKVLTHPRLLMIYTITALGYGGVFTAF-------TFLAPMMQELAG 232
+ G R+ + L R T+ A F ++
Sbjct: 183 ESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFH 242

Query: 233 FSPSAVSWILLGYGVSVAIGNVW-GGKLADKHGAVSALKFIFAALVVLLLVFQLTASVHY 291
+ + + L +G+ ++ G +A + G AL A + A+ +
Sbjct: 243 WDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGT-GYILLAFATRGW 301

Query: 292 AALATVLVMGIFAFGNVPGLQVYVVQKAEQYTPGAVDVASGLNIAAFNVGIALGSIVGGQ 351
A ++++ G +P LQ + ++ ++ G G A ++ +G ++
Sbjct: 302 MAFPIMVLLASGGIG-MPALQAMLSRQVDEERQGQ---LQGSLAALTSLTSIVGPLLFTA 357

Query: 352 TVERYGLAQTPWIG-AMIVLVALLLVVL 378
Y + T W G A I AL L+ L
Sbjct: 358 I---YAASITTWNGWAWIAGAALYLLCL 382


75KPHS_09290KPHS_09170Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_09290221-2.835753lipid-A-disaccharide synthase
KPHS_09280220-3.679069UDP-N-acetylglucosamine acyltransferase
KPHS_09270118-3.314692(3R)-hydroxymyristoyl-ACP dehydratase
KPHS_09260118-3.351812UDP-3-O-[3-hydroxymyristoyl] glucosamine
KPHS_09250016-2.603930chaperone protein Skp
KPHS_09240017-2.511174outer membrane protein assembly complex, YaeT
KPHS_09230-118-2.066987zinc metallopeptidase
KPHS_09220121-2.501311CDP-diglyceride synthase
KPHS_09210329-3.443638undecaprenyl pyrophosphate synthase
KPHS_09200230-3.4422791-deoxy-D-xylulose 5-phosphate reductoisomerase
KPHS_09190324-2.437232ribosome recycling factor
KPHS_09180223-2.084755uridylate kinase
KPHS_09170322-2.364975elongation factor Ts
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_09180CARBMTKINASE300.009 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 29.8 bits (67), Expect = 0.009
Identities = 18/66 (27%), Positives = 24/66 (36%), Gaps = 14/66 (21%)

Query: 120 AEAI-SLLRNNRVVILSAGTGNPFFTT-------------DSAACLRGIEIEADVVLKAT 165
AE I L+ +VI S G G P D A E+ AD+ + T
Sbjct: 176 AETIKKLVERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILT 235

Query: 166 KVDGVF 171
V+G
Sbjct: 236 DVNGAA 241


76KPHS_09070KPHS_08750Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_090700143.740772vitamin B12-transporter protein BtuF
KPHS_090600143.979823hypothetical protein
KPHS_090501164.274292hypothetical protein
KPHS_09040-1153.705372H(+)/Cl(-) exchange transporter ClcA
KPHS_090300163.993066glutamate-1-semialdehyde aminotransferase
KPHS_090200173.585888hydroxamate-dependent iron transport system
KPHS_090101151.851760iron-hydroxamate transporter substrate-binding
KPHS_090001140.742330iron-hydroxamate transporter ATP-binding
KPHS_08990214-0.340558ferrichrome outer membrane transporter
KPHS_08980-117-0.104008hypothetical protein
KPHS_08970-217-1.124546penicillin-binding protein 1b
KPHS_08960134-6.841920hypothetical protein
KPHS_08950013-1.482572hypothetical protein
KPHS_08940013-1.386820pullulanase secretion protein PulS
KPHS_08930-114-1.511909general secretion pathway protein B
KPHS_08920-113-1.563928hypothetical protein
KPHS_08910-1130.535566hypothetical protein
KPHS_08900-1152.314627pullulanase
KPHS_08890-2182.480652hypothetical protein
KPHS_08880-2183.501374pullulanase-specific type II secretion system
KPHS_088700204.910557pullulanase D protein
KPHS_088603237.035855pullulanase E protein
KPHS_088502246.854398pullulanase-specific type II secretion system
KPHS_088402237.045815pullulanase G protein
KPHS_088305237.838964pullulanase H protein
KPHS_088205228.033470pullulanase I protein
KPHS_088105217.907920general secretion pathway protein J
KPHS_088007198.562810pullulanase-specific type II secretion system
KPHS_087907198.663010pullulanase L protein
KPHS_087804186.791777general secretion pathway protein M
KPHS_087702145.624890pullulanase N protein
KPHS_087601134.993799pullulanase Q protein
KPHS_087501114.306958ATP-dependent helicase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_09070FERRIBNDNGPP481e-08 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 48.0 bits (114), Expect = 1e-08
Identities = 33/174 (18%), Positives = 61/174 (35%), Gaps = 9/174 (5%)

Query: 23 APRVITLSPANTELAFAAGITPVGVSSYSDY------PSQAKTIEQVASWQGMNLERIVA 76
R++ L EL A GI P GV+ +Y P ++ V NLE +
Sbjct: 35 PNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTE 94

Query: 77 LKPDVVLAWRG-GNAERQVNQLQSLGIHVLWVQTSTIEEIIATLRELAQWSPQPEKAQQA 135
+KP ++ G G + + ++ + +L E+A A+
Sbjct: 95 MKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETH 154

Query: 136 AQAMQQEYDALKARYANAPKKRVFL-QFGSAP-LFTSGPGSIQDQVLRLCGGEN 187
+ ++K R+ + + L + GP S+ ++L G N
Sbjct: 155 LAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPN 208


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_09010FERRIBNDNGPP432e-156 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 432 bits (1112), Expect = e-156
Identities = 214/291 (73%), Positives = 244/291 (83%)

Query: 6 LITRRRLLIAMALSPLLWQMRGAQAADVDPQRVVALEWLPAELLLALGVTPYGVADIPNY 65
LI+RRRLL AMALSPLLWQM A AA +DP R+VALEWLP ELLLALG+ PYGVAD NY
Sbjct: 6 LISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVADTINY 65

Query: 66 RLWVNEPALPDSVIDVGLRTEPNLELLTQMKPSFIVWSAGYGPSPEKLARIAPGRGFTFS 125
RLWV+EP LPDSVIDVGLRTEPNLELLT+MKPSF+VWSAGYGPSPE LARIAPGRGF FS
Sbjct: 66 RLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFS 125

Query: 126 DGKRPLAMAQRSLLEMADLLGKTQQAKRHLAEFDALMESLRPRFAGRGDRPLLMISLLDP 185
DGK+PLAMA++SL EMADLL A+ HLA+++ + S++PRF RG RPLL+ +L+DP
Sbjct: 126 DGKQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDP 185

Query: 186 RHVLVFGENCLFQEVLDRFGIKNAWHGEAAFWGSVSVGIDRLAAFNEADVICFDHGNERD 245
RH+LVFG N LFQE+LD +GI NAW GE FWGS +V IDRLAA+ + DV+CFDH N +D
Sbjct: 186 RHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKD 245

Query: 246 MAQLLATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFARVLADAQGSPA 296
M L+ATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHF RVL +A G A
Sbjct: 246 MDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVLDNAIGGKA 296


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08880BCTERIALGSPC2144e-71 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 214 bits (546), Expect = 4e-71
Identities = 100/266 (37%), Positives = 159/266 (59%), Gaps = 7/266 (2%)

Query: 17 KLLPQIVTLIILITAIPQLAKLTWRVVFPVSPEDISALPLTMPPAADPELKNVRPAFTLF 76
++ +I+ ++++ QLA + WR+ P ++ + + PA + FTLF
Sbjct: 12 SVIRRILFYLLMLLFCQQLAMIFWRIGLP---DNAPVSSVQITPAQARQQPVTLNDFTLF 68

Query: 77 GLAV-KNSPTPTDAASLNQVPVSSLKLRLAGLLASSNPARSIAIIEKGNQQVSLSTGDPL 135
G++ KN DA+ ++ +P S+L L L G++A + +RSIAII K N+Q S + +
Sbjct: 69 GVSPEKNKAGALDASQMSNLPPSTLNLSLTGVMAGDDDSRSIAIISKDNEQFSRGVNEEV 128

Query: 136 PGYDARIAAILPDRIIVNYQGRKEAILLFNDSRAPSPPPTAAGNPPLVKRLREQPQNILT 195
PGY+A+I +I PDR+++ YQGR E + L++ + S G + + +
Sbjct: 129 PGYNAKIVSIRPDRVVLQYQGRYEVLGLYSQEDSGSDGVP--GAQVNEQLQQRASTTMSD 186

Query: 196 YLSISPVLSGDKLLGYRLNPGKDASLFRQSGLQANDLAIALNGIDLRDQEQAQQALQNLA 255
Y+S SP+++ +KL GYRLNPG + F + GLQ ND+A+ALNG+DLRD EQA++A++ +A
Sbjct: 187 YVSFSPIMNDNKLQGYRLNPGPKSDSFYRVGLQDNDMAVALNGLDLRDAEQAKKAMERMA 246

Query: 256 DMTEITLTVEREGQRHDIAFAL-GDE 280
D+ TLTVER+GQR DI GDE
Sbjct: 247 DVHNFTLTVERDGQRQDIYMEFGGDE 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08870BCTERIALGSPD8380.0 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 838 bits (2167), Expect = 0.0
Identities = 606/646 (93%), Positives = 631/646 (97%)

Query: 10 ALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML 69
LLI LLF PAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML
Sbjct: 13 TLLIFAALLFRPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML 72

Query: 70 NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGEGDEVVTRVV 129
NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVR+KDAKT+AVPVAS AAPG GDEVVTRVV
Sbjct: 73 NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVV 132

Query: 130 PLTNVAARDLAPLLRQLNDNAGAGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR 189
PLTNVAARDLAPLLRQLNDNAG GSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR
Sbjct: 133 PLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR 192

Query: 190 SVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRI 249
SVVTVPLSWASAA+VVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRI
Sbjct: 193 SVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRI 252

Query: 250 IAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPVAAIDKNIII 309
IAMIKQLDRQQA QGNTKVIYLKYAKA+DLVEVLTGISS++QS+KQ+A+PVAA+DKNIII
Sbjct: 253 IAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKNIII 312

Query: 310 KAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKN 369
KAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKN
Sbjct: 313 KAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKN 372

Query: 370 AGMTQFTNSGLPISTAIAGANQYNKDGTISSSLASALGSFNGIAAGFYQGNWAMLLTALS 429
AGMTQFTNSGLPISTAIAGANQYNKDGT+SSSLASAL SFNGIAAGFYQGNWAMLLTALS
Sbjct: 373 AGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGNWAMLLTALS 432

Query: 430 SSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKP 489
SSTKNDILATPSIVTLDNM+ATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKP
Sbjct: 433 SSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKP 492

Query: 490 QINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKT 549
QINEGD+VLLEIEQEVSSVAD+ASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDK+
Sbjct: 493 QINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKS 552

Query: 550 VTDTADKVPLLGDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQASSGQYTAFN 609
V+DTADKVPLLGDIPVIGALFRS SKKVSKRNLMLFIRPT+IRDRDEYRQASSGQYTAFN
Sbjct: 553 VSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRDRDEYRQASSGQYTAFN 612

Query: 610 NAQTKQRGKESSEASLSNDLLHIYPQQETQAFRQVSAAIDAFNLGG 655
+AQ+KQRGKE+++A L+ DLL IYP+Q+T AFRQVSAAIDAFNLGG
Sbjct: 613 DAQSKQRGKENNDAMLNQDLLEIYPRQDTAAFRQVSAAIDAFNLGG 658


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08850BCTERIALGSPF5080.0 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 508 bits (1311), Expect = 0.0
Identities = 277/407 (68%), Positives = 334/407 (82%), Gaps = 4/407 (0%)

Query: 1 MALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLT-- 58
MA + YQALDAQGK RG Q+ADSAR ARQLLR++G + L V ++ L+
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 59 --RRTSAGDLALLTRQLATLVAAGIPLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSL 116
R S DLALLTRQLATLVAA +PLE+ALDAVA+Q EKP L LMA VRSKV+EGHSL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 117 AEAMRAYPACFDGLFYAMVAAGETSGHLDGVLNRLADYTEQRQQLRARLLQAMIYPIVLT 176
A+AM+ +P F+ L+ AMVAAGETSGHLD VLNRLADYTEQRQQ+R+R+ QAMIYP VLT
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 177 LVAISVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLA 236
+VAI+V++ILLS VVPKVVEQF+H+KQALP STR+LM +SD VR+ GPW+ L L +A
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 237 LRYLLRQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADV 296
R +LRQ RR+++ R LL LP+IGR+AR +N+ARYARTLSILNASAVPLL +MRIS DV
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 297 LSNAWARSQLAAASESVREGVSLHRALESTALFPPMMRYMIASGEQSGELTAMLERAAEN 356
+SN +AR +L+ A+++VREGVSLH+ALE TALFPPMMR+MIASGE+SGEL +MLERAA+N
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 357 QDRELSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNTLMS 403
QDRE S+Q+ +AL LFEPLLVV+MA +VLFIVLAILQPILQLNTLMS
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLMS 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08840BCTERIALGSPG2432e-86 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 243 bits (621), Expect = 2e-86
Identities = 98/140 (70%), Positives = 112/140 (80%)

Query: 1 MQRQRGFTLLEIMVVIVILGILASLVVPNLMGNKEKADRQKVVSDLVALEGALDMYKLDN 60
+QRGFTLLEIMVVIVI+G+LASLVVPNLMGNKEKAD+QK VSD+VALE ALDMYKLDN
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDN 63

Query: 61 SRYPNTEQGLQALVTAPAAEPHARNYPEGGYIRRLPQDPWGNEYQLLSPGQHGAIDVFSV 120
YP T QGL++LV AP P A NY + GYI+RLP DPWGN+Y L++PG+HGA D+ S
Sbjct: 64 HHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLLSA 123

Query: 121 GPDGMPDTNDDIGNWTLGKK 140
GPDG T DDI NW L KK
Sbjct: 124 GPDGEMGTEDDITNWGLSKK 143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08830BCTERIALGSPH1786e-60 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 178 bits (452), Expect = 6e-60
Identities = 99/164 (60%), Positives = 126/164 (76%)

Query: 1 MSQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQ 60
M QRGFTLLEMML+LLL+GVSA MVLLAFP++R A Q LARF+AQL FV++RG QTGQ
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQ 60

Query: 61 LFGIIIHPERWQFMRLQPADDSAPAAADDRWGNAQWLPLQAGRVTTAETLPRARLTLRFP 120
FG+ +HP+RWQF+ L+ D + PA ADD W +WLPL+AGRV T+ ++ +L L F
Sbjct: 61 FFGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAGRVATSGSIAGGKLNLAFA 120

Query: 121 DGQAWTPGEQPDVLIFPGGEVTPFQLRIDAAMGINVDAQGDSQP 164
G+AWTPG+ PDVLIFPGGE+TPF+L + A GI +A+G+S P
Sbjct: 121 QGEAWTPGDNPDVLIFPGGEMTPFRLTLGEAPGIAFNARGESLP 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08820BCTERIALGSPG322e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.2 bits (73), Expect = 2e-04
Identities = 22/99 (22%), Positives = 36/99 (36%), Gaps = 8/99 (8%)

Query: 1 MKREAGMTLIEVMVALVIF-ALAGLAV---MQSTLQQTRQLGRMEEKILASWLADNQLVQ 56
++ G TL+E+MV +VI LA L V M + + +Q + L + L +L
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDN 63

Query: 57 LRLEKRWPALS--WSETTVEAAGTRWFVRWQGVETALPQ 93
L T+ + +G LP
Sbjct: 64 HHYPTTNQGLESLVEAPTLPPLAANY--NKEGYIKRLPA 100


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08810BCTERIALGSPG334e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.6 bits (74), Expect = 4e-04
Identities = 20/61 (32%), Positives = 34/61 (55%), Gaps = 5/61 (8%)

Query: 6 RGFTLIETLLALAILAVLSAAAV-MVLQNVIRADGLTREKSQ-QIAALQRAFRQIADDVT 63
RGFTL+E ++ + I+ VL++ V ++ N +AD ++K+ I AL+ A D
Sbjct: 8 RGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKAD---KQKAVSDIVALENALDMYKLDNH 64

Query: 64 H 64
H
Sbjct: 65 H 65


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08760PREPILNPTASE2712e-93 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 271 bits (694), Expect = 2e-93
Identities = 138/277 (49%), Positives = 167/277 (60%), Gaps = 15/277 (5%)

Query: 1 MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRML---------------TQTA 45
M L L+ P++++ + LF L +GSF NVVI+RLP ML +
Sbjct: 1 MALLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGV 60

Query: 46 DDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWLGRRARCCQAPIAWSYPLTELATGLL 105
D+ L P S CP C PI +NIPLLS+LWL R R CQAPI+ YPL EL T LL
Sbjct: 61 DEPPYNLMVPRSCCPHCNHPITALENIPLLSWLWLRGRCRGCQAPISARYPLVELLTALL 120

Query: 106 FILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLNEVYIA 165
+ LAPG L+L L+ L ID LLPD+LTLPLLW GLLFNL +++
Sbjct: 121 SVAVAMTLAPGWGTLAALLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVS 180

Query: 166 LPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASA 225
L DAV GAMAGYL LWS+YW F+LLTGKE +GYGDFKLLAALGAW GWQ LP VLLL+S
Sbjct: 181 LGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL 240

Query: 226 SGLVWTLLQRLWTRQSLQQPLAFGPWLALAGGSIFLW 262
G + L +P+ FGP+LA+AG LW
Sbjct: 241 VGAFMGIGLILLRNHHQSKPIPFGPYLAIAGWIALLW 277


77KPHS_08640KPHS_08510Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_08640213-2.588596putative PTS enzyme II B component
KPHS_08630016-1.055743ABC transporter permease
KPHS_086200130.003093ATPase
KPHS_08610014-0.006374carbonate dehydratase
KPHS_08600-115-0.216807hypoxanthine-guanine phosphoribosyltransferase
KPHS_08590-215-0.578708hypothetical protein
KPHS_08580-114-1.004200glucose dehydrogenase
KPHS_08570119-2.384690multicopper oxidase
KPHS_08560633-5.752320hypothetical protein
KPHS_08550533-5.108705hypothetical protein
KPHS_08540429-4.072427hypothetical protein
KPHS_08530122-3.551896transposase InsC for insertion sequence IS903
KPHS_08520-118-4.166812hypothetical protein
KPHS_08510-216-4.194105transposase IS3/IS911 family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08630ABC2TRNSPORT725e-17 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 71.9 bits (176), Expect = 5e-17
Identities = 53/246 (21%), Positives = 108/246 (43%), Gaps = 4/246 (1%)

Query: 5 WVALKSIWTKEIHRFMRIWVQTLVPPVITMTLYFVIFGNLIGSRIGEMHGFTYMQFIVPG 64
W+A +W + + + + +L+ + +Y G +G +G + G +Y F+ G
Sbjct: 16 WIA---VWRRNYIAWKKAALASLLGHLAEPLIYLFGLGAGLGVMVGRVGGVSYTAFLAAG 72

Query: 65 LIMMAVITNA-YANVASSFFSAKFQRNIEELLVAPVPTHVIIAGYVGGGVARGLCVGILV 123
++ + +T A + + ++F + QR E +L + I+ G + + G +
Sbjct: 73 MVATSAMTAATFETIYAAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGI 132

Query: 124 TAVSLFFVPFQVHSWLFVALTLLLTAVLFSLAGLLNAVFAKTFDDISLIPTFVLTPLTYL 183
V+ Q S L+ + LT + F+ G++ A ++D T V+TP+ +L
Sbjct: 133 GVVAAALGYTQWLSLLYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFL 192

Query: 184 GGVFYSLTLLPPFWQGLSHLNPIVYMISGFRFGFLGINDVPLATTFAVLVVFIVAFYLLC 243
G + + LP +Q + P+ + I R LG V + L ++IV + L
Sbjct: 193 SGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLS 252

Query: 244 WSLIQR 249
+L++R
Sbjct: 253 TALLRR 258


78KPHS_07920KPHS_07750Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_07920012-3.972180isopropylmalate isomerase small subunit
KPHS_07910013-4.5000902,4-dienoyl-CoA reductase
KPHS_07900-110-3.703846shikimate 5-dehydrogenase
KPHS_07890012-4.288120MFS family transporter
KPHS_07880-115-4.1060343-dehydroquinate dehydratase
KPHS_07870013-2.444848putative LysR-family transcriptional regulator
KPHS_07860110-0.647849major facilitator superfamily permease
KPHS_078503122.425831sugar efflux protein
KPHS_078403133.011677hypothetical protein
KPHS_078304133.249677hypothetical protein
KPHS_078203143.297669transcriptional regulator SgrR
KPHS_078103143.271565thiamin/thiamine pyrophosphate ABC superfamily
KPHS_078003144.469420thiamine/thiamine pyrophosphate ABC superfamily
KPHS_077901143.191364thiamine transport system ATP-binding component
KPHS_07780-2142.589142hypothetical protein
KPHS_07760-3162.734184DNA-binding transcriptional regulator AraC
KPHS_077700202.905265hypothetical protein
KPHS_077500203.039099ribulokinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07890TCRTETA393e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 38.7 bits (90), Expect = 3e-05
Identities = 57/343 (16%), Positives = 112/343 (32%), Gaps = 8/343 (2%)

Query: 60 VTGFLSDRFGRKPFIYLGILSYLIFFVGILLTKNIYLAYVFGIMAGLANSFLDSGTYPAL 119
V G LSDRFGR+P + + + + + + +++ Y+ I+AG+ +
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA 121

Query: 120 MESFPHSASRANVLIKAFVSAGQFLLPFIISFLIWANLWFGWSFVIAAALFVLSGIYLLK 179
+ +R + A G P + + F AAAL L+ +
Sbjct: 122 DITDGDERARHFGFMSACFGFGMVAGPVLGGLM--GGFSPHAPFFAAAALNGLNFLTGCF 179

Query: 180 MPFPDSQAAKKEEAPTAQAETAVRPQANK-LDMVIFTLYGYIGMATFYLVSQWL-AQYGQ 237
+ P+S ++ + + + +V + + M V L +G+
Sbjct: 180 L-LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGE 238

Query: 238 FVVGL-PYASAIKLLSIYTVGSLVCVFVTAAFVKEVFSSAIAMIIYTGLSMISLLLVCLF 296
I L + + SL +T + M+ +LL
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFAT 298

Query: 297 PTPMMVTGFAFVIGFAAAGGVLQLGATIMAMSFPNGKGKATGIFYTAGSIASFTIPLITA 356
M + LQ A + +G+ G S+ S PL+
Sbjct: 299 RGWMAFPIMVLLASGGIGMPALQ--AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFT 356

Query: 357 KLSQISIASIMWFDFLIAVIGFVIALYIGYRQLQARAAQKVSR 399
+ SI + + ++ +++ L R L + A Q+ R
Sbjct: 357 AIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR 399


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07860TCRTETA613e-12 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 61.0 bits (148), Expect = 3e-12
Identities = 71/415 (17%), Positives = 132/415 (31%), Gaps = 46/415 (11%)

Query: 30 LSVGTMINYLDRTILGI---VAPQLSKEIHID---PAMMGIIFSAFAWTYALAQIPGGMF 83
L V LD +G+ V P L +++ A GI+ + +A G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 84 LDRFGNKVTYALSIFFWSLFTLLQSFTLGLKSLLLLRLGLGVSEAPCFPANSRIVSTWFP 143
DRFG + +S+ ++ + + L L + R+ G++ A ++
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGAT-GAVAGAYIADITD 125

Query: 144 QHERARA----TATYTVGEYIGLAAFSPLLFLILEHHGWRTLFFLTGGLGILFTLVWWRF 199
ERAR +A + G G P+L ++ FF L L L
Sbjct: 126 GDERARHFGFMSACFGFGMVAG-----PVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFL 180

Query: 200 YHEPHESRTANQAELEYIGASSINNKIQNVPFNWRDARRLLGCRQILGASLGQFAGNTTL 259
E H+ +N F W ++ + +
Sbjct: 181 LPESHKGERRPLRREA------LNPLAS---FRWARGMTVVAALMAVFFIMQLVGQ---- 227

Query: 260 VFFLTWFPSYLANERHLPWLHVGFFATWPFLAAAIGILFGGWISDRLLKRTGSVNISRKL 319
+ + + H +G + L I+ + R G
Sbjct: 228 -VPAALWVIFGEDRFHWDATTIGISLA---AFGILHSLAQAMITGPVAARLGERRA---- 279

Query: 320 PIISGLLLSSC--IIAANWVSANSTVIIIMSVAFFGQGMVGLGWTLISDIAPENMAGLTG 377
++ G++ I+ A I++ +A G GM L ++S E G
Sbjct: 280 -LMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQ-AMLSRQVDEERQGQLQ 337

Query: 378 GIFNFCANMASIIAPLIIGVIISATGNFFYALIYVGLTALIGVIAYIFIIGDIKR 432
G ++ SI+ PL+ I +A+ + G + G Y+ + ++R
Sbjct: 338 GSLAALTSLTSIVGPLLFTAIYAAS-----ITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07850TCRTETA330.002 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.9 bits (75), Expect = 0.002
Identities = 41/187 (21%), Positives = 67/187 (35%), Gaps = 17/187 (9%)

Query: 16 AAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAIAGILVSLWLAKRSDS 75
AA M V F+M + G + A +F +G+ I L + +
Sbjct: 213 AALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAA 272

Query: 76 RGDRRRLIMFCCLMAVGNALLFAFNRHYLTLITCGVMLASIANAAMPQLFALAREYADSS 135
R RR +M + +L AF V+LAS MP L A+ D
Sbjct: 273 RLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGG-IGMPALQAMLSRQVDEE 331

Query: 136 AREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTTMFSIAAG-----IFVISLALIAI 190
+ + S SL ++GP L FT +++ + ++ AL +
Sbjct: 332 RQGQLQGSLAALT--SLTSIVGPLL---------FTAIYAASITTWNGWAWIAGAALYLL 380

Query: 191 KLPSVPR 197
LP++ R
Sbjct: 381 CLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07820NEISSPPORIN372e-04 Neisseria sp. porin signature.
		>NEISSPPORIN#Neisseria sp. porin signature.

Length = 348

Score = 36.5 bits (84), Expect = 2e-04
Identities = 17/81 (20%), Positives = 30/81 (37%), Gaps = 2/81 (2%)

Query: 367 YHAGEHYQ-GNWFPAYGLLPRWHHASNHACEKPAGLETVTLTYYRDHVEHRVIGGIMRDL 425
YH G +YQ +F Y L + + E ++ + HR++GG +
Sbjct: 180 YHVGLNYQNSGFFAQYAGLFQRYGEGTKKIEYDDQTYSIPSLFVEKLQVHRLVGGYDNNA 239

Query: 426 LTAHQVKLEIQELEYDAWHRG 446
L V + Q+ + G
Sbjct: 240 LYV-SVAAQQQDAKLYGAMSG 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07780PHPHTRNFRASE280.048 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 27.8 bits (62), Expect = 0.048
Identities = 19/76 (25%), Positives = 33/76 (43%), Gaps = 2/76 (2%)

Query: 95 RALLEKTEHALHQHSMITILIGRFVGPTRPLVPMVAGMLDLPVAKFVLPNIIGCLLWPPL 154
R LEK + Q + +L G + + PM+A + +L AK ++ LL +
Sbjct: 362 RLCLEKQDIFRTQ--LRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGV 419

Query: 155 YFLPGILAGAAIDIPA 170
I G ++IP+
Sbjct: 420 DVSDSIEVGIMVEIPS 435


79KPHS_06820KPHS_06560Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_06820020-3.400128ribosomal-protein-alanine N-acetyltransferase
KPHS_06810018-2.784543DNA polymerase III subunit psi
KPHS_06800014-3.47624616S ribosomal RNA m2G1207 methyltransferase
KPHS_06790122-5.411704tryptophan-specific transport protein
KPHS_06780024-5.901870hypothetical protein
KPHS_06770-223-5.327658putative transmembrane protein
KPHS_06760-224-4.404954ferric hydroximate transport ferric iron
KPHS_06750-222-3.621807chitibiose transporter ChbC
KPHS_06740027-4.637096hypothetical protein
KPHS_06730-123-3.9746712-component transcriptional regulator
KPHS_06720-121-3.006568hypothetical protein
KPHS_06710217-1.302835putative AraC-type regulatory protein
KPHS_06700214-1.1858424-hydroxybenzoate 3-monooxygenase
KPHS_06690416-2.713172hypothetical protein
KPHS_06680-110-2.167923paral putative membrane protein
KPHS_06670-111-2.274868hypothetical protein
KPHS_06660-211-2.667760primosomal protein I
KPHS_06650-211-2.929833DNA replication protein DnaC
KPHS_06640-210-2.681890putative outer membrane protein
KPHS_06630-210-2.525447phosphoglycerol transferase I
KPHS_06620-115-3.409608putative cytoplasmic protein
KPHS_06610015-4.664943putative glucosamine-fructose-6-phosphate
KPHS_06600-117-3.345189putative glucosamine-fructose-6-phosphate
KPHS_06590-118-3.438777PTS system mannose/fructose/sorbose transporter
KPHS_06580-120-3.921951putative PTS permease
KPHS_06570-120-4.558807putative PTS permease
KPHS_06560-217-4.064829putative PTS permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06820SACTRNSFRASE481e-09 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 47.6 bits (113), Expect = 1e-09
Identities = 22/80 (27%), Positives = 35/80 (43%), Gaps = 1/80 (1%)

Query: 62 DEATLFNIAVDPAYQRRGLGRALLEHVIDEVEKLGVVTLWLEVRASNVAAIALYESVGFN 121
A + +IAV Y+++G+G ALL I+ ++ L LE + N++A Y F
Sbjct: 88 GYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI 147

Query: 122 EATIRRNYYP-TADGREDAI 140
+ Y E AI
Sbjct: 148 IGAVDTMLYSNFPTANEIAI 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_067602FE2SRDCTASE372e-133 Ferric iron reductase signature.
		>2FE2SRDCTASE#Ferric iron reductase signature.

Length = 262

Score = 372 bits (955), Expect = e-133
Identities = 171/262 (65%), Positives = 209/262 (79%)

Query: 1 MAWRSLPLSDELIWRAPLPTAEHALAESIREKIATLRPHLLDFLRLDEPAPRHALTLAEW 60
MA+RS PL +++IWR L + LA+++R IA R HLL+F+RLDEPAP +A+TLA+W
Sbjct: 1 MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFIRLDEPAPLNAMTLAQW 60

Query: 61 SQPIALRSLLATWSDHIYRHQPTLPREQKPLLSLWAQWYIGLLVPPLMLALLNEPQGLSL 120
S P L SLLA +SDHIYR+QP + RE KPL+SLWAQWYIGL+VPPLMLALL + + L +
Sbjct: 61 SSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGLMVPPLMLALLTQEKALDV 120

Query: 121 APEHFHVEFHESGRAACFWIDVHSDADIERLSPQARMDALVTRTLQPVVEALAATGEINS 180
+PEHFH EFHE+GR ACFW+DV D + SPQ RM+ L+++ L PVV+AL ATGEIN
Sbjct: 121 SPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQHRMETLISQALVPVVQALEATGEING 180

Query: 181 KLIWSNTGYLINWYLGEMRALLGDERLAALRQHCFFEKQLADGQDNPLWRTVMLREGQLV 240
KLIWSNTGYLINWYL EM+ LLG+ + +LR FFEK L +G+DNPLWRTV+LR+G LV
Sbjct: 181 KLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTNGEDNPLWRTVVLRDGLLV 240

Query: 241 RRTCCQRYRLPDVQQCGDCTLK 262
RRTCCQRYRLPDVQQCGDCTLK
Sbjct: 241 RRTCCQRYRLPDVQQCGDCTLK 262


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06620FLGFLIH352e-04 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 35.2 bits (80), Expect = 2e-04
Identities = 15/38 (39%), Positives = 23/38 (60%)

Query: 239 PQYEETLMSIAQKLKQEGRQQGRLEGREEGHLEGLQEG 276
P E+ L + + ++G Q G EGR++GH +G QEG
Sbjct: 38 PSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEG 75



Score = 29.0 bits (64), Expect = 0.023
Identities = 12/22 (54%), Positives = 17/22 (77%)

Query: 255 EGRQQGRLEGREEGHLEGLQEG 276
EGRQQG +G +EG +GL++G
Sbjct: 62 EGRQQGHKQGYQEGLAQGLEQG 83


80KPHS_06470KPHS_06240Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_064702190.072070keto-hydroxyglutarate-aldolase/keto-deoxy-
KPHS_064602160.5625132-oxo-3-deoxygalactonate kinase
KPHS_064502161.220436sodium/panthothenate symporter
KPHS_064402162.300136hypothetical protein
KPHS_064302163.278004hypothetical protein
KPHS_064204193.059648hypothetical protein
KPHS_064103193.446850hypothetical protein
KPHS_064004213.624299putative dehydrogenase
KPHS_063901171.529405hypothetical protein
KPHS_063801161.903504hypothetical protein
KPHS_063701160.5952624-hydroxyphenylacetate catabolism
KPHS_063601200.678117hypothetical protein
KPHS_063502222.0636254-hydroxyphenylacetate catabolism
KPHS_063400242.2183704-hydroxyphenylacetate degradation bifunctional
KPHS_06330-1211.9632394-hydroxyphenylacetate catabolism
KPHS_06320-1171.4728734-hydroxyphenylacetate catabolism
KPHS_063100191.6325034-hydroxyphenylacetate catabolism
KPHS_06300-1171.5219992-oxo-hepta-3-ene-1,7-dioic acid hydratase
KPHS_06290-113-0.5869254-hydroxyphenylacetate catabolism
KPHS_06280010-3.2719534-hydroxyphenylacetate permease
KPHS_06270010-2.5507554-hydroxyphenylacetate catabolism regulatory
KPHS_06260014-3.3609114-hydroxyphenylacetate catabolism
KPHS_06250014-4.2339724-hydroxyphenylacetate catabolism
KPHS_06240115-4.329326ascBF operon repressor
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06430UREASE502e-08 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 49.7 bits (119), Expect = 2e-08
Identities = 29/106 (27%), Positives = 49/106 (46%), Gaps = 17/106 (16%)

Query: 3 VDWLFKNVTVIDGSGGPQYRADVAVKGDRIMAIAPA--------LDV---AAEQVIDGQG 51
VD + N ++D G +AD+ +K RI AI A + + +VI G+G
Sbjct: 68 VDTVITNALILDHWG--IVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEG 125

Query: 52 RVLAPGFIDVHTHDDINVIRMPEYLPKLSQGVTTVIVGNCGISAAT 97
+++ G +D H H I ++ E L G+T ++ G G + T
Sbjct: 126 KIVTAGGMDSHIH-FICPQQIEE---ALMSGLTCMLGGGTGPAHGT 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06290PHPHTRNFRASE310.004 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 31.3 bits (71), Expect = 0.004
Identities = 30/173 (17%), Positives = 56/173 (32%), Gaps = 39/173 (22%)

Query: 78 QIKQLLDVGAQT---LLVPMVQNAEEARLAVRATRYPPAGIRGVGSALARASRWNRVPDY 134
Q++ LL ++ PM+ EE R A + + G ++ +
Sbjct: 374 QLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDVSDSIEVG----- 428

Query: 135 IHRANDAMCVLVQIETREALKNLPQILDVDGVDGVFIGPADL----------SADMGHGG 184
+ +E VD IG DL + + +
Sbjct: 429 -----------IMVEIPSTAVAANLFAKE--VDFFSIGTNDLIQYTMAADRMNERVSYLY 475

Query: 185 NPQHPEVQAAIEDAIQQIRQAGKAPGIL--MANEQLAKRYLELGALFVAVGVD 235
P HP + ++ I+ GK G+ MA +++A L + +G+D
Sbjct: 476 QPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIP------LLLGLGLD 522


81KPHS_05840KPHS_05260Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_05840-1213.023507copper/silver efflux system outer membrane
KPHS_058300233.542969copper-binding protein
KPHS_058200233.930667RND family efflux transporter MFP subunit
KPHS_058101234.551387hypothetical protein
KPHS_058001256.518318cyanate transport
KPHS_057902266.511088lipoprotein Blc
KPHS_057802277.031111MerR family transcriptional regulator
KPHS_057703277.827071hypothetical protein
KPHS_057604278.114523oxidoreductase
KPHS_057503277.362188L-aspartate oxidase
KPHS_057403276.780337plasmid partition ParA protein
KPHS_057300275.320061cyclopropane-fatty-acyl-phospholipid synthase
KPHS_05710-1254.101455hypothetical protein
KPHS_05720-2211.710187hypothetical protein
KPHS_05700-117-0.822619putative lipoprotein
KPHS_05690216-1.000113hypothetical protein
KPHS_05680313-1.835752hypothetical protein
KPHS_05670111-2.175998hypothetical protein
KPHS_05660011-2.305735xylose isomerase
KPHS_05650313-4.651057hypothetical protein
KPHS_05640418-5.868692putative beta-1,4-xylosidase, XynB
KPHS_05630436-9.810270hypothetical protein
KPHS_05620337-12.363356hypothetical protein
KPHS_05610652-16.051037hypothetical protein
KPHS_05600651-15.456782hypothetical protein
KPHS_05590341-12.969480hypothetical protein
KPHS_05580119-2.452811Hcp1 family type VI secretion system effector
KPHS_05570020-0.999811phospholipase D
KPHS_05560-120-0.154592IS3 family element, transposase orfB
KPHS_05550-2211.092596hypothetical protein
KPHS_055400201.305895IS3 family element, transposase orfA
KPHS_055300212.939176nucleoside triphosphatase, D5 family
KPHS_055203253.923194P4 phage protein
KPHS_055100212.634094hypothetical protein
KPHS_05500-2212.809244phage immunity repressor protein
KPHS_05490021-0.001941phage DNA binding protein
KPHS_05480028-6.926530hypothetical protein
KPHS_05470139-10.616562phage transcriptional activator, Ogr/delta
KPHS_05460349-14.916929phage polarity suppression protein
KPHS_05450254-15.620335hypothetical protein
KPHS_05440047-13.009907transposase InsC for insertion sequence IS903
KPHS_05430134-9.015827hypothetical protein
KPHS_05420-118-3.569337hypothetical protein
KPHS_05410-115-2.266688hypothetical protein
KPHS_054000152.153012putative P4-type integrase
KPHS_053903285.312027putative carbonic anhdrase
KPHS_053802264.657942putative sulfate transporter
KPHS_053700263.235868putative alcohol dehydrogenase
KPHS_053600252.424087putative SN-glycerol-3-phosphate transport
KPHS_05350-1241.710165sn-glycerol 3-phosphate transport system
KPHS_05340-1231.643055putative phosphohydrolase
KPHS_05330-1242.545750sugar ABC transporter
KPHS_05320-1231.804595ABC transporter substrate-binding protein
KPHS_05310-1222.825136putative sugar phosphate isomerase/epimerase
KPHS_05300-1202.940467protein IolH
KPHS_05290-1203.540262putative NADH-dependent dehydrogenase
KPHS_05280-1193.625182putative acetolactate synthase large subunit
KPHS_05270-1152.845723hypothetical protein
KPHS_05260-1183.6897495-dehydro-2-deoxygluconokinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05840GPOSANCHOR310.009 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 31.2 bits (70), Expect = 0.009
Identities = 29/166 (17%), Positives = 58/166 (34%), Gaps = 9/166 (5%)

Query: 140 RLKNLSEADRQNFFASEEARRAVHILLIANVSQSYFNQRLAAAQLQVANDTLQNYQQSYA 199
A + A + A A L + + +A+++ + A
Sbjct: 204 NFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQA 263

Query: 200 FVEKQLLTGSTTVLALEQARSMIESTRADIAKRQGQLAQANNALQLLLGSYQHLPDDSAS 259
+EK L + + I++ A+ A + + A + Q+L + Q L D +
Sbjct: 264 ELEKAL---EGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDA 320

Query: 260 SAVDLQGVTLPPSLSSAILLQRPDILEAEHSLQAANANIGAARAAF 305
S + + L ++ I EA S Q+ ++ A+R A
Sbjct: 321 SREAKKQL----EAEHQKLEEQNKISEA--SRQSLRRDLDASREAK 360


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05810ACRIFLAVINRP6880.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 688 bits (1777), Expect = 0.0
Identities = 224/1059 (21%), Positives = 437/1059 (41%), Gaps = 54/1059 (5%)

Query: 1 MIEWIIRRSVANRFLVMMAALFLSIWGTWTIIHTPVDALPDLSDVQVIVKTRYPGQAPQI 60
M + IRR + A+ L + G I+ PV P ++ V V YPG Q
Sbjct: 1 MANFFIRR----PIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQT 56

Query: 61 VENQVTWPLTTTMLSVPGARTVRGFSQ-FGDSYVYVIFEDGTDPYWARSRVLEYLNQVQG 119
V++ VT + M + + S G + + F+ GTDP A+ +V L
Sbjct: 57 VQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATP 116

Query: 120 KLPAGVSAEMGP-DATGVGWVFEYALVDRSGKHDLAELRSLQDWFLKYELKTIPNVSEVA 178
LP V + + + ++ V + ++ +K L + V +V
Sbjct: 117 LLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQ 176

Query: 179 SVGGVVKEYQIVVDPMKLTQYGISLGEVKSALDASNQEAGGSSVELA------EAEYMVR 232
G +I +D L +Y ++ +V + L N + + + +
Sbjct: 177 LFGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASII 235

Query: 233 ASGYLQTLDDFKNIVLKTGDNGVPVYLGDVARVQIGPEMRRGIAELNGEGEVAGGVVILR 292
A + ++F + L+ +G V L DVARV++G E IA +NG+ AG + L
Sbjct: 236 AQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGK-PAAGLGIKLA 294

Query: 293 SGKNAREVISAVKEKLASLQSSLPEGVEVVTTYDRSQLIDRAIDNLSYKLLEEFIVVALV 352
+G NA + A+K KLA LQ P+G++V+ YD + + +I + L E ++V LV
Sbjct: 295 TGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLV 354

Query: 353 CALFLWHVRSALVAIISLPLGLCFAFIMMHFQGLNANIMSLGGIAIAVGAMVDAAIVMIE 412
LFL ++R+ L+ I++P+ L F ++ G + N +++ G+ +A+G +VD AIV++E
Sbjct: 355 MYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVE 414

Query: 413 NAHKRLEEWEHQHPGEKLSNDTRWKIITEASVEVGPALFISLLIITLSFIPIFTLEGQEG 472
N + + E + P + ++ ++ AL ++++ FIP+ G G
Sbjct: 415 NVERVMME-DKLPP---------KEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTG 464

Query: 473 KLFGPLAFTKTWSMAGAALLAIVAIPILMGFWIRGRIPAENSNPLNRF----------LI 522
++ + T +MA + L+A++ P L ++ AE+ F +
Sbjct: 465 AIYRQFSITIVSAMALSVLVALILTPALCATLLKPV-SAEHHENKGGFFGWFNTTFDHSV 523

Query: 523 RIYHPLLLKVLHWPKTTLLIALLSILTVVWPLNRVGGEFLPQINEGDLLYMPSTLPGISA 582
Y + K+L LLI L + +V R+ FLP+ ++G L M G +
Sbjct: 524 NHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQ 583

Query: 583 AQAADMLQKTDKLIMA--VPEVARVFGKTGKAETATDSAPLEMVETTIQLKPQDQW-RPG 639
+ +L + + V VF G + + + LKP ++
Sbjct: 584 ERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQN---AGMAFVSLKPWEERNGDE 640

Query: 640 MTMEKIVDELDKTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTNLADIDAIAGQ 699
+ E ++ + + + +++ + I +G + Q
Sbjct: 641 NSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQ 700

Query: 700 IEGVARSVPG-VTSALAERLVGGRYLDIDIQREKAARYGMTVGDVQLFVSSAIGGAMVGE 758
+ G+A P + S L +++ +EKA G+++ D+ +S+A+GG V +
Sbjct: 701 LLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVND 760

Query: 759 TVEGVERYPINIRYPQSYRDSPETLRQLPILTPLKQQIVLGDVAEVKVVTGPSMLKTENA 818
++ + ++ +R PE + +L + + + + V G L+ N
Sbjct: 761 FIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNG 820

Query: 819 RPTSWIYIDARDRDMVSVVHDLQQAIGKEVKLKPGISVSYSGQFELLERANQKLKLMVPM 878
P+ I +A L + + KL GI ++G + + +V +
Sbjct: 821 LPSMEIQGEAAPGTSSGDAMALMENL--ASKLPAGIGYDWTGMSYQERLSGNQAPALVAI 878

Query: 879 TLMIIFVLLYLAFRRVGEALLIITSVPFALVGGIWFLYWMGFHLSVATGTGFIALAGVAA 938
+ +++F+ L + + ++ VP +VG + V G + G++A
Sbjct: 879 SFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSA 938

Query: 939 EFGVVMLMYLRHAIEAEPSLENPQTFSVDKLDEALYQGAVLRVRPKAMTVAVIIAGLLPI 998
+ ++++ + + +E E + EA +R+RP MT I G+LP+
Sbjct: 939 KNAILIVEFAKDLMEKEGK----------GVVEATLMAVRMRLRPILMTSLAFILGVLPL 988

Query: 999 LWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKL 1037
GAGS + + ++GGM++A LL++F +P + +
Sbjct: 989 AISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVV 1027


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05790BCTLIPOCALIN2331e-81 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 233 bits (595), Expect = 1e-81
Identities = 85/151 (56%), Positives = 111/151 (73%), Gaps = 1/151 (0%)

Query: 25 PKGVQPISGFDASRYLGKWYEVARLENRFERGLEQVTATYGARSDGGISVVNRGYDPVKK 84
P+ V+P+S F+ + YLGKWYEVARL++ FERGL QVTA Y R+DGGISV+NRGY K
Sbjct: 20 PESVKPVSDFELNNYLGKWYEVARLDHSFERGLSQVTAEYRVRNDGGISVLNRGYSEEKG 79

Query: 85 RWNESDGKAYFTGAPTTAALKVSFFGPFYGGYNVIRLD-DDYQYALVSGPNRDYLWILSR 143
W E++GKAYF T LKVSFFGPFYG Y V LD ++Y YA VSGPN +YLW+LSR
Sbjct: 80 EWKEAEGKAYFVNGSTDGYLKVSFFGPFYGSYVVFELDRENYSYAFVSGPNTEYLWLLSR 139

Query: 144 TPTIPAAVKQDYLNTARELGFDVDRLVWIRQ 174
TPT+ + ++ ++E GFD +RL++++Q
Sbjct: 140 TPTVERGILDKFIEMSKERGFDTNRLIYVQQ 170


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05760DHBDHDRGNASE622e-13 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 61.6 bits (149), Expect = 2e-13
Identities = 51/233 (21%), Positives = 85/233 (36%), Gaps = 16/233 (6%)

Query: 4 VLITGASSGIGAGLAKSFAADGHLVIACGRDASRLAALQQLSPNISVRL-----FDMTDR 58
ITGA+ GIG +A++ A+ G + A + +L + S R D+ D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVS-SLKAEARHAEAFPADVRDS 69

Query: 59 DACRQALTGCFA-----DLIILCAGTCEYLDHGQVDAALVERVMATNFLGPVNCLAALQT 113
A + D+++ AG + E + N G N ++
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129

Query: 114 QLEA--GDRVVLVSSMAHWLPFPRAEAYGASKAALTWFANSLRLDWEPKGVAVTVVSPGF 171
+ +V V S +P AY +SKAA F L L+ + +VSPG
Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189

Query: 172 VDTPLTRKNDFAMPGRVSVDRAVAA-IRHGLAKGKNHIAFPTGFSLALRLLAS 223
+T + G V + + G+ K +A P+ + A+ L S
Sbjct: 190 TETDMQWSLWADENGAEQVIKGSLETFKTGIPLKK--LAKPSDIADAVLFLVS 240


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_055202FE2SRDCTASE260.037 Ferric iron reductase signature.
		>2FE2SRDCTASE#Ferric iron reductase signature.

Length = 262

Score = 25.8 bits (56), Expect = 0.037
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 2/49 (4%)

Query: 18 VACAWLTLCERQHRYPHLTLDALESAIATELEGFY--LRQHGEEKGRLI 64
VAC W+ +CE ++ PH +E+ I+ L L GE G+LI
Sbjct: 135 VACFWVDVCEDKNATPHSPQHRMETLISQALVPVVQALEATGEINGKLI 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05330PF05272356e-04 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 34.7 bits (79), Expect = 6e-04
Identities = 14/33 (42%), Positives = 18/33 (54%)

Query: 30 VVLVGPSGCGKSTLLRLLAGLEPVSEGQIWLHD 62
VVL G G GKSTL+ L GL+ S+ +
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGT 631


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05320MALTOSEBP409e-06 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 40.5 bits (94), Expect = 9e-06
Identities = 85/307 (27%), Positives = 133/307 (43%), Gaps = 36/307 (11%)

Query: 120 LQKEFWPAMHKNAQVMGTTYAIPFHNSTPILYYNKTMFDQAGIKQPPQTWAELLADAKKL 179
Q + +P + G A P L YNK + + PP+TW E+ A K+L
Sbjct: 111 FQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDL-----LPNPPKTWEEIPALDKEL 165

Query: 180 TDESKGQWGIMLPSTNDDYGGWIFSALVRANGG---KYFNEDYP-GEVYYNSPTAIGALR 235
++KG+ +M + + Y W L+ A+GG KY N Y +V ++ A L
Sbjct: 166 --KAKGKSALMF-NLQEPYFTW---PLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLT 219

Query: 236 FWQDLIYKDKVMPSGVLNSKQISAAFFSGKLGMAMLSTGALGFMRENSKDFELGVAMLPA 295
F DLI K+K M + + AAF G+ M + G + ++ GV +LP
Sbjct: 220 FLVDLI-KNKHMNADT-DYSIAEAAFNKGETAMTI--NGPWAWSNIDTSKVNYGVTVLPT 275

Query: 296 -KEQRAVPIGGASLVSFKGINEA--QKKAAYQFL-TYLVSPEVNGAWSRFTGYFSPRKAS 351
K Q + P G V GIN A K+ A +FL YL++ E A ++ + S
Sbjct: 276 FKGQPSKPFVG---VLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKS 332

Query: 352 YDTPEMKAYLQQDPRAAIALEQLKYAHPWYSTWETVAVRKAMENQLAAVVNDA--KVTPE 409
Y + L +DPR A +E + + + A A+ AV+N A + T +
Sbjct: 333 Y-----EEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVR---TAVINAASGRQTVD 384

Query: 410 AAVQAAQ 416
A++ AQ
Sbjct: 385 EALKDAQ 391


82KPHS_05140KPHS_05070Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_05140-220-4.618969hydroxylase for synthesis of
KPHS_05130-327-6.020132hypothetical protein
KPHS_05120-230-6.685942ornithine carbamoyltransferase
KPHS_05110-139-8.411919GntR family transcriptional regulator
KPHS_05100-135-7.238108C4-dicarboxylate anaerobic carrier
KPHS_05090-227-5.986559hypothetical protein
KPHS_05080-217-5.320977ribokinase-like domain-containing protein
KPHS_05070-117-3.984468sugar isomerase (SIS)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05090UREASE372e-04 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 36.6 bits (85), Expect = 2e-04
Identities = 14/30 (46%), Positives = 21/30 (70%)

Query: 462 WTLNSARHHGMEEMTGSLEPGKRADIAVFD 491
+T+N A HG+ GSLE GKRAD+ +++
Sbjct: 409 YTINPAIAHGLSHEIGSLEVGKRADLVLWN 438


83KPHS_04310KPHS_04230Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_043102133.077597transcriptional repressor UlaR
KPHS_043001102.620084putative hydrolase
KPHS_04290091.918052hypothetical protein
KPHS_042802121.484537hypothetical protein
KPHS_042701121.497814isovaleryl CoA dehydrogenase
KPHS_04260316-0.79794823S rRNA (guanosine-2'-O-)-methyltransferase
KPHS_04250217-1.197942ribonuclease R
KPHS_04240424-3.216530transcriptional repressor NsrR
KPHS_04230224-1.473321adenylosuccinate synthetase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_04270ACRIFLAVINRP300.037 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.8 bits (67), Expect = 0.037
Identities = 25/80 (31%), Positives = 31/80 (38%), Gaps = 11/80 (13%)

Query: 11 QPTPLNNSNLFLSD--TALREAVVREGAGWDGDLLASIGQQLGTAESLELGRLANSNPPE 68
LN L D L+ + AG G A GQQL A + R NP E
Sbjct: 189 DADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQL-NASIIAQTRFK--NPEE 245

Query: 69 L----LRYDATGA--RLDDV 82
LR ++ G+ RL DV
Sbjct: 246 FGKVTLRVNSDGSVVRLKDV 265


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_04250IGASERPTASE330.008 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.7 bits (74), Expect = 0.008
Identities = 27/103 (26%), Positives = 47/103 (45%), Gaps = 2/103 (1%)

Query: 709 RVEAVNMDERKIDFTLISSERAPRNVGKTAREKAKKSTSGKPGGRRRQVGKQVNFEPDSA 768
E V + ++ T+ +E+ RE AK++ S + Q E
Sbjct: 1036 TTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKET 1095

Query: 769 FRKE-KETARPKKEKKAKKPSAKTQKIAAATKAKRAAKKKIAE 810
E KETA +KE+KAK + KTQ++ T ++ + K++ +E
Sbjct: 1096 QTTETKETATVEKEEKAKVETEKTQEVPKVT-SQVSPKQEQSE 1137


84KPHS_03990KPHS_03810Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_03990218-0.601068fumarate reductase iron-sulfur subunit
KPHS_03980320-0.657574fumarate reductase, cytochrome b subunit
KPHS_03970121-1.490340fumarate reductase subunit D
KPHS_03960017-0.797797outer membrane lipoprotein Blc
KPHS_03950021-4.989618quarternary ammonium compound transport protein
KPHS_03940128-6.148108entericidin B membrane lipoprotein
KPHS_03930029-7.171702entericidin A
KPHS_03920224-6.364026elongation factor P
KPHS_03910332-8.544758putative aminomutase
KPHS_03900539-9.913880hypothetical protein
KPHS_03890544-11.442903type 1 fimbrial protein
KPHS_03880243-11.981868fimbrial biogenesis periplasmic chaperone
KPHS_03870242-12.014024putative fimbrial biogenesis outer membrane
KPHS_03860250-14.089250hypothetical protein
KPHS_03850040-10.545272hypothetical protein
KPHS_03840040-10.794536microcin H47 secretion protein
KPHS_03830135-8.356954microcin H47 ABC transporter,
KPHS_03820226-3.223937hypothetical protein
KPHS_03810229-1.295068putative lipoprotein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03960BCTLIPOCALIN2502e-88 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 250 bits (640), Expect = 2e-88
Identities = 71/152 (46%), Positives = 104/152 (68%), Gaps = 1/152 (0%)

Query: 25 PPGVTVVSPFDVQRYLGTWYEIARFDHPFESGLEKVTIAWHPRDDGGLDVVNKGYNPDRG 84
P V VS F++ YLG WYE+AR DH FE GL +VT + R+DGG+ V+N+GY+ ++G
Sbjct: 20 PESVKPVSDFELNNYLGKWYEVARLDHSFERGLSQVTAEYRVRNDGGISVLNRGYSEEKG 79

Query: 85 MWQKTDGVAYFTGEPSRAALKISFFGPFYGSYNVIALDKE-YRYALVCGPDRDYLWLLAR 143
W++ +G AYF + LK+SFFGPFYGSY V LD+E Y YA V GP+ +YLWLL+R
Sbjct: 80 EWKEAEGKAYFVNGSTDGYLKVSFFGPFYGSYVVFELDRENYSYAFVSGPNTEYLWLLSR 139

Query: 144 APTIAPEVRQQMLDIATRQGFDVGKLVWVNQR 175
PT+ + + ++++ +GFD +L++V Q+
Sbjct: 140 TPTVERGILDKFIEMSKERGFDTNRLIYVQQQ 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03870PF005776970.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 697 bits (1800), Expect = 0.0
Identities = 298/812 (36%), Positives = 447/812 (55%), Gaps = 58/812 (7%)

Query: 3 NGRYLDTRTIKFVANNRASSDNREPALVPCLSLKALAEYGVRIKAFPELA-EDQNGCANF 61
N Y+ TR + F + +VPCL+ LA G+ + + + C
Sbjct: 85 NNGYMATRDVTFNTGDSEQG------IVPCLTRAQLASMGLNTASVSGMNLLADDACVPL 138

Query: 62 -SVIPDTKADFDFTAQRLNISIPQAALSTTAQGYIPPDQFDDGINALLVNYQFSGS---N 117
S+I D A D QRLN++IPQA +S A+GYIPP+ +D GINA L+NY FSG+ N
Sbjct: 139 TSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN 198

Query: 118 DMQANDEYYSLNLQSGLNVGPWRIRNLSTWNKNNS-----GAGDWDSAYLYMQRSIRSIN 172
+ N Y LNLQSGLN+G WR+R+ +TW+ N+S W +++R I +
Sbjct: 199 RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLR 258

Query: 173 SNLVMGESSSLNGIFDSVPFTGIQLATDTTMLPESMRGYAPIIRGIARTNARVIIKQNGY 232
S L +G+ + IFD + F G QLA+D MLP+S RG+AP+I GIAR A+V IKQNGY
Sbjct: 259 SRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGY 318

Query: 233 QVYQTYVAPGAFEITDMYPSGGSGDLYVTVEESDGSKQEFVVPFATLPVMVREDQLEYEI 292
+Y + V PG F I D+Y +G SGDL VT++E+DGS Q F VP++++P++ RE Y I
Sbjct: 319 DIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSI 378

Query: 293 TSGKYRPYDGGVDETPFTQATATYGVSSSLTLYGGMQAASRYQALSTGLGYNLGELGAAS 352
T+G+YR + ++ F Q+T +G+ + T+YGG Q A RY+A + G+G N+G LGA S
Sbjct: 379 TAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALS 438

Query: 353 ADVTQAWSKKKEDEKTSGQSWRVRYGKNIVETGTNVTIAGYRYSTRGFNTLSEVLDSYSN 412
D+TQA S +D + GQS R Y K++ E+GTN+ + GYRYST G+ ++ S N
Sbjct: 439 VDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMN 498

Query: 413 DGS------------------YTSRSLRNRTNLTLNQSLGKGLGSLSISGLIEDYWDDKR 454
+ + + R + LT+ Q LG+ +L +SG + YW
Sbjct: 499 GYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGR-TSTLYLSGSHQTYWGTSN 557

Query: 455 TNKSISVGYNGGFRNVNYYLGYSYNRYTWSGDNSGKDAQDDQRITLTVTLPLSNWLPG-- 512
++ G N F ++N+ L YS + W DQ + L V +P S+WL
Sbjct: 558 VDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR-------DQMLALNVNIPFSHWLRSDS 610

Query: 513 ------TYTSYQLTNSNPGSTDQSVSIGGNALENDSLEWSLHQGYSNRE----YYSGDMR 562
SY +++ G + G LE+++L +S+ GY+ +G
Sbjct: 611 KSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYAT 670

Query: 563 ATYNGARGSVNAGYSYDNNSQRIDYGANGSILAHADGITLGQDITDAAVLVKAPGLDDVR 622
Y G G+ N GYS+ ++ +++ YG +G +LAHA+G+TLGQ + D VLVKAPG D +
Sbjct: 671 LNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAK 730

Query: 623 LANDNTISTDYRGYAIVPYVTPYRRTDITLDSTTLGEDMELPETTKSVVPTRGAIVRASY 682
+ N + TD+RGYA++PY T YR + LD+ TL ++++L +VVPTRGAIVRA +
Sbjct: 731 VENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEF 790

Query: 683 DGNIGQRAFVHLKTASGQDVPYGAMVLLVGDSKSQPSIVSDAGMVYMSGLQQTGILNVQW 742
+G + + L T + + +P+GAMV IV+D G VY+SG+ G + V+W
Sbjct: 791 KARVGIKLLMTL-THNNKPLPFGAMVTSESS--QSSGIVADNGQVYLSGMPLAGKVQVKW 847

Query: 743 GKSAAQQCNASFTLPTREGKASGIRQIETICR 774
G+ C A++ LP + + Q+ CR
Sbjct: 848 GEEENAHCVANYQLPPESQQQ-LLTQLSAECR 878


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03840RTXTOXIND1183e-31 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 118 bits (297), Expect = 3e-31
Identities = 72/430 (16%), Positives = 149/430 (34%), Gaps = 62/430 (14%)

Query: 29 PAWLVTCLSLLFLCALICALIFCKFTQRIDVKGEVITLPHSVNVFSPQQGFVVNQYVQIG 88
P + + + A I + + + G++ S + + V V+ G
Sbjct: 57 PRLVAYFIMGFLVIAFILS-VLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEG 115

Query: 89 DVVKKGQTLYELDVSRNTTTGNVSAAQIEVINEKIAN----------------------- 125
+ V+KG L +L + Q ++ ++
Sbjct: 116 ESVRKGDVLLKLTALG--AEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 126 ------SEAIIKKLTHNKNETLIALDAQLKNARNSLNET-------VRMLANTQQGLSKM 172
SE + +LT E Q +L++ + + +
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 173 HENLSSYDKYLKEGLITKDQYNYQHSLYFQQQSAYQSLISQKMQLETQLTQLSSDKVTKA 232
L + L + I K Q + Y + + + SQ Q+E+++ +
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 233 ADFDNQISSQYNQTND----YKNQLVESNAN-GNIIIKATTEGRIESLAV-TKGQMVDKG 286
F N+I + QT D +L ++ +I+A +++ L V T+G +V
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 287 SSLAQIKPIGNIEYYLILWLPNNSIPYVKVGDTINIRYDAFPSDKFGQFPGEIISISSLP 346
+L I P + + + N I ++ VG I+ +AFP ++G G++ +I+
Sbjct: 354 ETLMVIVPEDD-TLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINL-- 410

Query: 347 ASRQEMSEYTNVNDGTNQQELAL-YKAIVKIRDKKFNYDGKELSLSNGLKAQAVVFLEER 405
D Q L L + I+ I + + K + LS+G+ A + R
Sbjct: 411 -------------DAIEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMR 457

Query: 406 PLYMWMFTPV 415
+ ++ +P+
Sbjct: 458 SVISYLLSPL 467


85KPHS_03450KPHS_03320Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_03450-1233.938466putative alkylphosphonate uptake protein in
KPHS_03440-1234.644035hypothetical protein
KPHS_034301235.013411putative transcriptional regulator
KPHS_034200224.845856phosphonate C-P lyase system protein
KPHS_034102224.723777carbon-phosphorus lyase complex subunit
KPHS_034000224.571551phosphonate metabolism protein
KPHS_033901204.053684protein in phn operon
KPHS_033802184.131260phosphonates transport ATP-binding protein
KPHS_033703195.334975phosphonate transport ATP-binding protein
KPHS_033604195.345054phosphonate metabolism protein PhnM
KPHS_033503205.133578phosphonate transport ATP-binding protein
KPHS_033402204.826940carbon-phosphorus lyase complex accessory
KPHS_033302195.065818hypothetical protein
KPHS_033200143.550801putative histidine protein kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03370PF05272280.027 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.027
Identities = 14/42 (33%), Positives = 19/42 (45%)

Query: 36 CVVLHGHSGSGKSTLLRSLYANYLPDSGHIHIRHGDEWVDLV 77
VVL G G GKSTL+ +L H I G + + +
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQI 639


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03330RTXTOXIND300.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.8 bits (67), Expect = 0.002
Identities = 24/115 (20%), Positives = 47/115 (40%), Gaps = 12/115 (10%)

Query: 13 LSLTSLAARADIIDDAIGNIQQAINDAYNPGSSRSDDDDRYDDDGRYDDGRYQGS----- 67
L LT+L A AD + +Q + SRS + ++ + D+ +Q
Sbjct: 125 LKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEV 184

Query: 68 -------RQQSRDSQRQYDERQRQLDERRRQLDERQRQLDRDRRQLESDQRRLDD 115
++Q Q Q +++ LD++R + +++R ++ RLDD
Sbjct: 185 LRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDD 239


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03320HTHFIS564e-10 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 55.6 bits (134), Expect = 4e-10
Identities = 28/121 (23%), Positives = 58/121 (47%), Gaps = 8/121 (6%)

Query: 646 LVLEDEEDVRQTLCEQLHQLGWLTLETASGEEALQLLEASPDIALLISDLMLPGALSGAD 705
LV +D+ +R L + L + G+ T++ + + A L+++D+++P + D
Sbjct: 7 LVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD-GDLVVTDVVMPDE-NAFD 64

Query: 706 VIHTARRRFPALPVLLISGQDLRPAQNPALPE--VEWLRKPF----TRAQLAQALSAAYA 759
++ ++ P LPVL++S Q+ A + ++L KPF + +AL+
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 760 R 760
R
Sbjct: 125 R 125


86KPHS_03170KPHS_03120Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_03170113-3.036584hypothetical protein
KPHS_03160213-3.300160acetate permease
KPHS_03150322-5.073420putative adhesin
KPHS_03140221-4.542004fimbrial morphology protein
KPHS_03130220-4.525066putative outer membrane usher protein
KPHS_03120-121-4.459077putative chaperone
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03150BCTERIALGSPD290.042 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 28.7 bits (64), Expect = 0.042
Identities = 26/120 (21%), Positives = 44/120 (36%), Gaps = 12/120 (10%)

Query: 155 QLQLVATGGAIASKSQLSFSDPVATVSAKDKKGTIAISQLHISGTTSIQLIPMGCIVGSN 214
Q G + S L S +A + +K GT++ S S G G
Sbjct: 367 QWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVS------SSLASALSSFNGIAAGFY 420

Query: 215 NLSFSMGSINASEFNTATKVGSARQSLSLSCEP-----GTNVSMRVAAASASGDNPDNTV 269
+++M + A +T + + ++L G V + + + SGDN NTV
Sbjct: 421 QGNWAM-LLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTV 479


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03130PF005776560.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 656 bits (1695), Expect = 0.0
Identities = 286/780 (36%), Positives = 432/780 (55%), Gaps = 60/780 (7%)

Query: 2 INTTAYPQL-FEAGETCARL-SAIPGMTFSVSLAQQRIDFTVPQAAMLNRPRDYIPESQW 59
+NT + + A + C L S I T + + QQR++ T+PQA M NR R YIP W
Sbjct: 119 LNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELW 178

Query: 60 QQGINAGLLNYSVTGQRNAPRHNGATIDSQFVSLQPGLNLGPWRLRNYSTYSHSDNNS-- 117
GINAGLLNY+ +G R G +++LQ GLN+G WRLR+ +T+S++ ++S
Sbjct: 179 DPGINAGLLNYNFSGNSVQNR-IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSS 237

Query: 118 ----RWESVYSYLARDIHTLRSQLVVGNTYTSSGIFDSLSFTGLQLSSDKEMLPDSLHGF 173
+W+ + ++L RDI LRS+L +G+ YT IFD ++F G QL+SD MLPDS GF
Sbjct: 238 GSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGF 297

Query: 174 APTIRGIARTTAEVSVYQNGYSIYKTTVAPGAFEINDLYATGSAGDLYVNIKESDGSEQN 233
AP I GIAR TA+V++ QNGY IY +TV PG F IND+YA G++GDL V IKE+DGS Q
Sbjct: 298 APVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQI 357

Query: 234 FVVPFASLAILQREGQLDYALSSGRTRSGSSDDKEYNFIQSSLAYGATSNITLYTGFQQA 293
F VP++S+ +LQREG Y++++G RSG++ ++ F QS+L +G + T+Y G Q A
Sbjct: 358 FTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA 417

Query: 294 EDKYTNLLLGAGFNLGTIGALSFDGSQSWADVKTSDTASSTSKEQGQSYRVRFSKSFLQT 353
+ Y G G N+G +GALS D +Q+ + + S+ GQS R ++KS ++
Sbjct: 418 DR-YRAFNFGIGKNMGALGALSVDMTQANSTLP------DDSQHDGQSVRFLYNKSLNES 470

Query: 354 GTSFSVAGYRYSTSGYYSFQDFVD----------------NSSTQRDCCTQSGRTKGRFD 397
GT+ + GYRYSTSGY++F D D + +G+
Sbjct: 471 GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQ 530

Query: 398 ASLSQTLFGYGSLSLSLVNETYWDSS-RMESVGVGYSGSIGKASYFINYSYNRNVQSTDD 456
+++Q L +L LS ++TYW +S E G + + ++ ++YS +N
Sbjct: 531 LTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNA----- 585

Query: 457 SSNNRPSSDTVVSLTLSIPLGETL-----------SANYTLNHGRHNDTTHSVGLNGSAF 505
+ D +++L ++IP L SA+Y+++H + T+ G+ G+
Sbjct: 586 ---WQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLL 642

Query: 506 EDRSLNWSVLEGYNTQDKSTSGN---LSVNYQGSKGDVAGGYGYDRYSNHYNYSLRGGMV 562
ED +L++SV GY SG+ ++NY+G G+ GY + Y + GG++
Sbjct: 643 EDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL 702

Query: 563 AHAGGLTLSRFLGESSALVETPGVSDVTVRGQTNVTTDAAGYAVVPYVRPYHRNSLALDE 622
AHA G+TL + L ++ LV+ PG D V QT V TD GYAV+PY Y N +ALD
Sbjct: 703 AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDT 762

Query: 623 QEIP-GAEVDNVARTVVPTRNAIVKVKYDTRIGYKAMLTLRTRNGVVPFGALVTLDNDHG 681
+ ++DN VVPTR AIV+ ++ R+G K ++TL N +PFGA+VT ++
Sbjct: 763 NTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQ- 821

Query: 682 SASRSNIVGDEGQVYLTGLQKKGQLLARWGEKSSEQCTVHYDFSGMALGDDILFYQAECR 741
S IV D GQVYL+G+ G++ +WGE+ + C +Y + + AECR
Sbjct: 822 ---SSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR 878


87KPHS_02590KPHS_02530Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_02590015-3.248821maltose/maltodextrin transporter ATP-binding
KPHS_02580117-3.853603maltose ABC transporter periplasmic protein
KPHS_02570116-4.044401maltose transporter membrane protein
KPHS_02560221-4.753053maltose transporter permease
KPHS_02550424-5.852741phosphate-starvation-inducible protein PsiE
KPHS_02540427-5.144100hypothetical protein
KPHS_02530321-2.979931putative amino acid transport protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_02590PF05272340.001 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 33.5 bits (76), Expect = 0.001
Identities = 13/35 (37%), Positives = 18/35 (51%)

Query: 32 VVFVGPSGCGKSTLLRMIAGLETITSGDLFIGDTR 66
VV G G GKSTL+ + GL+ + IG +
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGK 633


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_02580MALTOSEBP7560.0 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 756 bits (1952), Expect = 0.0
Identities = 378/396 (95%), Positives = 391/396 (98%)

Query: 1 MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK 60
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK
Sbjct: 1 MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK 60

Query: 61 VSVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW 120
V+VEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW
Sbjct: 61 VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW 120

Query: 121 DAVRYNGKLIAYPIAVEALSLIYNKDLVPNPPKTWEEIPALDKELKAKGKSALMFNLQEP 180
DAVRYNGKLIAYPIAVEALSLIYNKDL+PNPPKTWEEIPALDKELKAKGKSALMFNLQEP
Sbjct: 121 DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP 180

Query: 181 YFTWPLIAADGGYAFKFENGKYDVKDVGVDSAGAKAGLTFLVDLIKNKHMNADTDYSIAE 240
YFTWPLIAADGGYAFK+ENGKYD+KDVGVD+AGAKAGLTFLVDLIKNKHMNADTDYSIAE
Sbjct: 181 YFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE 240

Query: 241 AAFNKGETAMTINGPWAWSNIDKSKVNYGVTLLPTFKGKPSKPFVGVLSAGINAASPNKE 300
AAFNKGETAMTINGPWAWSNID SKVNYGVT+LPTFKG+PSKPFVGVLSAGINAASPNKE
Sbjct: 241 AAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE 300

Query: 301 LAKEFLENYLMTDQGLEAVNNDKPLGAVALKSFQEKLEKDPRIAATMANAQKGEIMPNIP 360
LAKEFLENYL+TD+GLEAVN DKPLGAVALKS++E+L KDPRIAATM NAQKGEIMPNIP
Sbjct: 301 LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP 360

Query: 361 QMSAFWYAVRTAVINAASGRQTVDAALKDAQSRITK 396
QMSAFWYAVRTAVINAASGRQTVD ALKDAQ+RITK
Sbjct: 361 QMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK 396


88KPHS_00550KPHS_00430Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_00550-1123.818910putative glycoprotein
KPHS_005402172.883498galETK operon repressor
KPHS_005304200.910963putative helix-turn-helix regulatory protein
KPHS_00520420-2.038709hypothetical protein
KPHS_00510321-4.007187putative inner membrane protein
KPHS_00500324-5.759634putative serine protease
KPHS_00490331-8.929757PTS family protein IIA component
KPHS_00480332-9.084325hypothetical protein
KPHS_00470228-6.778907putative ketose-bisphosphate aldolase, class-II
KPHS_00460225-5.588102putative sugar phosphotransferase component IIC
KPHS_00450117-3.707043PTS family protein II component
KPHS_00440115-2.308454putative 6-phosphofructokinase
KPHS_00430216-0.496573hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_00530HTHTETR300.006 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 29.6 bits (66), Expect = 0.006
Identities = 8/29 (27%), Positives = 16/29 (55%)

Query: 27 AKSLVRERARTGLSLAEVARRAGIAKSTL 55
A L ++ + SL E+A+ AG+ + +
Sbjct: 20 ALRLFSQQGVSSTSLGEIAKAAGVTRGAI 48


89KPHS_00330KPHS_00170Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_00330118-3.200416putative acetyltransferase
KPHS_00320328-3.751233D-tyrosyl-tRNA deacylase
KPHS_00310125-3.275147ribonuclease BN
KPHS_00300121-2.468389phosphatase
KPHS_00290018-2.395299GTP-binding protein
KPHS_00280-213-2.096752hypothetical protein
KPHS_00270-214-1.850616glutamine synthetase
KPHS_00260013-1.374606nitrogen regulation protein NR(II)
KPHS_00250014-2.189108nitrogen regulation protein NR(I)
KPHS_00240014-3.042904coproporphyrinogen III oxidase
KPHS_00230014-3.088173hypothetical protein
KPHS_00220015-3.553597GTP-binding protein
KPHS_00210-115-3.518996DNA polymerase I
KPHS_00200-221-5.584101putative endonuclease
KPHS_00190024-5.174591protein disulfide isomerase I
KPHS_00180-123-4.157264serine/threonine protein kinase RdoA
KPHS_00170022-3.490276hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_00290TCRTETOQM1811e-51 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 181 bits (462), Expect = 1e-51
Identities = 100/448 (22%), Positives = 172/448 (38%), Gaps = 87/448 (19%)

Query: 4 NLRNIAIIAHVDHGKTTLVDKLLQQSGTFD--ARTEAQERVMDSNDLEKERGITILAKNT 61
+ NI ++AHVD GKTTL + LL SG + D+ LE++RGITI T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 62 AIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAFDGPMPQTRFVTKKAFAHGL 121
+ +W + ++NI+DTPGH DF EV R +S++D +L++ A DG QTR + G+
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 122 KPIVVINKVDRPGARPDWVVDQVFD-------------LFVNLDATDEQLD--------- 159
I INK+D+ G V + + L+ N+ T+
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 160 --------------------------------FPIVYASALNGIAGLDHEDMADDMTPLY 187
FP+ + SA N I G+D+ L
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNI-GIDN---------LI 231

Query: 188 QAIVDRVPAPDVDLDGPLQMQISQLDYNNYVGVIGIGRIKRGKVKPNQQVTIIDSEGKTR 247
+ I ++ + L ++ +++Y+ + R+ G + V I + E
Sbjct: 232 EVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-- 289

Query: 248 NGKVGKVLTHLGLERIESDVAEAGDIIAITGLG-ELN--ISDTICDPQNVEALPALSVDE 304
K+ ++ T + E + D A +G+I+ + +LN + DT PQ +
Sbjct: 290 --KITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQR----ERIENPL 343

Query: 305 PTVSMFFNVNTSPFCGKEGKFVTSRQILDRLNKELVHNVALRVEETEDADAFRVSGRGEL 364
P + + + D L LR +S G++
Sbjct: 344 PLLQTTVEPSKPQQREMLLDALLEISDSDPL---------LRYYVDSATHEIILSFLGKV 394

Query: 365 HLSVLIENMRRE-GFEMAVSRPKVIFRE 391
+ V ++ + E+ + P VI+ E
Sbjct: 395 QMEVTCALLQEKYHVEIEIKEPTVIYME 422



Score = 30.6 bits (69), Expect = 0.019
Identities = 12/75 (16%), Positives = 29/75 (38%), Gaps = 1/75 (1%)

Query: 398 EPFENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIGFRSEFMT 457
EP+ + + +++ + ++ + V L IP+R + +RS+
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKN-NEVILSGEIPARCIQEYRSDLTF 595

Query: 458 MTSGTGLLYSTFSHY 472
T+G + + Y
Sbjct: 596 FTNGRSVCLTELKGY 610


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_00250HTHFIS6000.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 600 bits (1549), Expect = 0.0
Identities = 203/478 (42%), Positives = 297/478 (62%), Gaps = 11/478 (2%)

Query: 1 MQRGIAWIVDDDSSIRWVLERALTGAGLSCTTFESGNEVLDALTTKTPDVLLSDIRMPGM 60
M + DDD++IR VL +AL+ AG + + + D++++D+ MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVDRAIS 120
+ LL +IK+ P LPV++M+A + A+ A ++GA+DYLPKPFD+ E + ++ RA++
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 HYQEQQQPRNAPISSPTADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA 180
+ + ++G + AMQ+++R++ RL ++ ++++I GESGTGKELVA A
Sbjct: 121 EPKRRPSKLEDDSQDG-MPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARA 179

Query: 181 LHRHSPRSKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTVRQGRFEQADGGTLFLDE 240
LH + R PF+A+NMAAIP+DLIESELFGHEKGAFTGA T GRFEQA+GGTLFLDE
Sbjct: 180 LHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDE 239

Query: 241 IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHR 300
IGDMP+D QTRLLRVL G++ VGG P++ DVRI+AAT+++L+Q + +G FREDL++R
Sbjct: 240 IGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYR 299

Query: 301 LNVIRVHLPPLRERREDIPRLARHFLQIAARELGVEAKQLHPETETALTRLAWPGNVRQL 360
LNV+ + LPPLR+R EDIP L RHF+Q A +E G++ K+ E + WPGNVR+L
Sbjct: 300 LNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALELMKAHPWPGNVREL 358

Query: 361 ENTCRWLTVMAAGQEVLTQDLPSELFETTIPDSPTQMQPDSWATLLGQWADRALRS---- 416
EN R LT + + + + +EL + S + + Q + +R
Sbjct: 359 ENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFAS 418

Query: 417 -----GHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME 469
L EME L+ AL T+G++ +AA LLG RNTL +K++ELG+
Sbjct: 419 FGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_00230SECA280.022 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 27.9 bits (62), Expect = 0.022
Identities = 12/57 (21%), Positives = 23/57 (40%)

Query: 15 KSREELNQEARDRKRQKKHRGHAAGSRANGGDAASAGKKQRQAQDPRVGSKKPIPLG 71
+ EE+ + + R+ + + D+A+A Q + +VG P P G
Sbjct: 832 RMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCG 888


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_00200PRPHPHLPASEC290.024 Prokaryotic zinc-dependent phospholipase C signature.
		>PRPHPHLPASEC#Prokaryotic zinc-dependent phospholipase C signature.

Length = 398

Score = 28.8 bits (64), Expect = 0.024
Identities = 11/31 (35%), Positives = 13/31 (41%)

Query: 69 NPWLKWDVQGLEGLNKKNWYLLISNHHSWAD 99
N W K +G K +Y S HSW D
Sbjct: 214 NAWSKEYARGFAKTGKSIYYSHASMSHSWDD 244


90KPHS_52880KPHS_52810N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_528800110.969978putative membrane / transport protein
KPHS_528702121.909392putative oxidoreductase, flavoprotein
KPHS_528600131.148332hypothetical protein
KPHS_52850-1131.187592LysR family transcriptional regulator
KPHS_52840-1141.081149putative membrane transport protein
KPHS_52830-1160.331905hypothetical protein
KPHS_52820-117-0.207222tRNA modification GTPase
KPHS_52810017-1.414044putative inner membrane protein translocase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52880TYPE3IMSPROT310.013 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 30.5 bits (69), Expect = 0.013
Identities = 22/114 (19%), Positives = 43/114 (37%), Gaps = 3/114 (2%)

Query: 331 LTAVVVGILFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVKWSDLTEAVPA--- 387
L+ VV +L PL + A A ++ G L++ + + A
Sbjct: 72 LSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKKINPIEGAKRI 131

Query: 388 FITAVMMPFSFSITEGIALGFISYCVMKIGTGRLRELSPCVIIVSLLFVLKIVF 441
F ++ F SI + + L + + ++K L +L C I + +I+
Sbjct: 132 FSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQILR 185


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52840TCRTETA582e-11 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 58.3 bits (141), Expect = 2e-11
Identities = 66/311 (21%), Positives = 118/311 (37%), Gaps = 14/311 (4%)

Query: 5 LLCSFALVLLYPSGIDMYLVGLPRIAQDLGASEAQLHIAFSVYLAGMASAML----FAGR 60
L+ + V L GI + + LP + +DL S + + + LA A G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSN-DVTAHYGILLALYALMQFACAPVLGA 65

Query: 61 IADRSGRKPVAIVGAAIFVIASLLCAQAHTSSHFLIGRFIQGIGAGSCYVVAFAILRDTL 120
++DR GR+PV +V A + + A A IGR + GI G+ VA A + D
Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADIT 124

Query: 121 DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKYPWQSLFYTMTGMGVMVAVLSVFILRE 180
D RA+ ++ V PVLG L M + + F+ + + + F+L E
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGL-MGGFSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 181 TRPTAPPQAASPQHDAGESLLNRFFLSRLLITTLSVTVILTYVNVSPVLMMEEMGFDRGT 240
+ + S + ++ ++V I+ V P + G DR
Sbjct: 184 SHKGERRPLRREALNPLASFRWARGM-TVVAALMAVFFIMQLVGQVPAALWVIFGEDRFH 242

Query: 241 YSMAM------ALMAMISMAVSFSTPFALSLFNPRTLMLTSQVLFLAAGVTLSLATRQAV 294
+ A + S+A + T + R ++ + + L+ ATR +
Sbjct: 243 WDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWM 302

Query: 295 TLIGLGMICAG 305
+ ++ +G
Sbjct: 303 AFPIMVLLASG 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52830SACTRNSFRASE383e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.0 bits (88), Expect = 3e-06
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 3/55 (5%)

Query: 69 IVDVAVDPAHQGKGLGRLVMEKLVAWLDANAFDGAYV-TLVADVP--ELYAKFGF 120
I D+AV ++ KG+G ++ K + W N F G + T ++ YAK F
Sbjct: 92 IEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_5281060KDINNERMP7870.0 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 787 bits (2034), Expect = 0.0
Identities = 461/532 (86%), Positives = 493/532 (92%), Gaps = 2/532 (0%)

Query: 1 MIWQAWEQDKNPQPQ-QQTTQTTTTAAGSAADQGVPASGQGKLITVKTDVLELTINTNGG 59
MIWQAWEQDKNPQPQ QQTTQTTTTAAGSAADQGVPASGQGKLI+VKTDVL+LTINT GG
Sbjct: 18 MIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKLISVKTDVLDLTINTRGG 77

Query: 60 DIEQALLLAYPKTLKSTEPFQLLETTPQFVYQAQSGLTGRDGPDNPANGPRPLYNVDKEA 119
D+EQALL AYPK L ST+PFQLLET+PQF+YQAQSGLTGRDGPDNPANGPRPLYNV+K+A
Sbjct: 78 DVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDA 137

Query: 120 FVLADGQDELVIPLTYTDKAGNVFTKTFTLKRGGYAVNVGYSVQNASEKPLEVSTFGQLK 179
+VLA+GQ+EL +P+TYTD AGN FTKTF LKRG YAVNV Y+VQNA EKPLE+S+FGQLK
Sbjct: 138 YVLAEGQNELQVPMTYTDAAGNTFTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLK 197

Query: 180 QTAALPTSRDTQTGGLSTMHTFRGAAFSTADSKYEKYKFDTILDNENLNVSTKNGWVAML 239
Q+ LP DT + + +HTFRGAA+ST D KYEKYKFDTI DNENLN+S+K GWVAML
Sbjct: 198 QSITLPPHLDTGSSNFA-LHTFRGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAML 256

Query: 240 QQYFTTAWVPRNNGTNNFYTANLGNGIVAIGYKSQPVLVQPGQTDKLQSTLWVGPAIQDK 299
QQYF TAW+P N+GTNNFYTANLGNGI AIGYKSQPVLVQPGQT + STLWVGP IQDK
Sbjct: 257 QQYFATAWIPHNDGTNNFYTANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDK 316

Query: 300 MAAVAPHLDLTVDYGWLWFISQPLFKLLKFIHSFLGNWGFSIIVITFIVRGIMYPLTKAQ 359
MAAVAPHLDLTVDYGWLWFISQPLFKLLK+IHSF+GNWGFSII+ITFIVRGIMYPLTKAQ
Sbjct: 317 MAAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQ 376

Query: 360 YTSMAKMRMLQPKIQAMRERLGDDKQRQSQEMMALYKAEKVNPLGGCFPLIIQMPIFLAL 419
YTSMAKMRMLQPKIQAMRERLGDDKQR SQEMMALYKAEKVNPLGGCFPL+IQMPIFLAL
Sbjct: 377 YTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLAL 436

Query: 420 YYMLSASVELRHAPFILWIHDLSAQDPYYILPIIMGATMFFIQKMSPTTVTDPMQQKIMT 479
YYML SVELR APF LWIHDLSAQDPYYILPI+MG TMFFIQKMSPTTVTDPMQQKIMT
Sbjct: 437 YYMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMT 496

Query: 480 FMPVIFTVFFLWFPSGLVVYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS 531
FMPVIFTVFFLWFPSGLV+YYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS
Sbjct: 497 FMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS 548


91KPHS_52430KPHS_52360N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_52430-2132.094142DNA-binding transcriptional activator UhpA
KPHS_52420-1120.626062two-component regulatory system sensor histidine
KPHS_52410-112-0.663060regulatory protein UhpC
KPHS_52400-210-0.733715hexose phosphate transport protein
KPHS_52390-112-0.402298hypothetical protein
KPHS_52380-1120.017165putative helix-turn-helix protein
KPHS_52370-1100.882374RelE family toxin-antitoxin system
KPHS_52360-1132.307189putative inner membrane transport protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52430HTHFIS642e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.5 bits (157), Expect = 2e-14
Identities = 24/116 (20%), Positives = 46/116 (39%), Gaps = 5/116 (4%)

Query: 2 TTIALIDDHLIVRSGFAQLLGLEADFQVVAEFGSGREALTGLPGRGVQVCICDISMPDIS 61
TI + DD +R+ Q L + V + + + + D+ MPD +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRA-GYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 62 GLELLSQLPK---GMATIMLSVHDSPALIEQALNAGARGFLSKRCSPDELIAAVRT 114
+LL ++ K + +++S ++ +A GA +L K ELI +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52410TCRTETB371e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 36.8 bits (85), Expect = 1e-04
Identities = 65/407 (15%), Positives = 130/407 (31%), Gaps = 58/407 (14%)

Query: 29 RHILLTIWLGYALFY--FTRKSFNAAVPEILASNVLTRSDIGLLATLFYITYGLSKFFSG 86
RH + IWL F+ N ++P+I + + T F +T+ + G
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 87 IVSDRSDARYFMGLGLIATGVVNILFGFSSSLWAFALLWALNAFFQGWGS---PVCARLL 143
+SD+ + + G+I +++ S F L + F QG G+ P ++
Sbjct: 71 KLSDQLGIKRLLLFGIIINCFGSVIGFVGHS---FFSLLIMARFIQGAGAAAFPALVMVV 127

Query: 144 TAWY-SRTERGGWWALWNTAHNVGGALIPMVVGAAALHYGWRAGMTIAGCLAILAGLYLC 202
A Y + RG + L + +G + P + G A + W + I I
Sbjct: 128 VARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITV----- 182

Query: 203 WRLRDRPQAVGLPAVGDWRHDALEIAQQQEGAGMSRKAILTRYVLANPYIWLLSLCYVLV 262
P + L +I G + I+ + Y + VL
Sbjct: 183 ------PFLMKLLKKEVRIKGHFDIK----GIILMSVGIVFFMLFTTSYSISFLIVSVLS 232

Query: 263 YVV-----RAAINDWGNLYMSETLGVDLVTANSAVTMFELGGFI-----------GALVA 306
+++ R + + + + + + + + + GF+ A
Sbjct: 233 FLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA 292

Query: 307 GWGSDKLFNGNRGPMNLIFAAGILLSVGGLWLMPFASYVMQAACFFTTGFFVFGPQMLI- 365
GS +F G + + GIL+ G + + F T F + +
Sbjct: 293 EIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMT 352

Query: 366 --------GMAAAECS---------HKEAAGAATGFVGLFAYLGASL 395
G++ + ++ AGA + ++L
Sbjct: 353 IIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT 399


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52400TCRTETB357e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.9 bits (80), Expect = 7e-04
Identities = 27/168 (16%), Positives = 62/168 (36%), Gaps = 17/168 (10%)

Query: 49 FNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFMLILSAIC 108
N++ D+ + + + F +T+ +G + +D K+ L F +I++
Sbjct: 33 LNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIIN--- 89

Query: 109 MLGFSASMGAGSTSLFLMIAFYALSGFFQSTGGSCSYSTI----TKWTPRRKRGTFLGFW 164
F + +G S F ++ + F Q G + + + ++ P+ RG G
Sbjct: 90 --CFGSVIGFVGHSFFSLLIM---ARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLI 144

Query: 165 NISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRY 212
+G + A+Y+ + + + P + I+ L
Sbjct: 145 GSIVAMGEGVGPAIGGMIAHYIHWSY---LLLIP--MITIITVPFLMK 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52360TCRTETA320.003 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.5 bits (74), Expect = 0.003
Identities = 27/151 (17%), Positives = 53/151 (35%), Gaps = 3/151 (1%)

Query: 65 AFVAMFSSLFITTVIGKTDRRYVVILFSLLLTLSCLLVSFADSFTLLLLGRACLGLALGG 124
A + + + + + RR V+++ + +++ A +L +GR G+ G
Sbjct: 53 ALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGIT-GA 111

Query: 125 FWAMSASLTMRLVPMRVVPKALSIIFGAVSIALVIAAPLGSFLGGLIGWRNVFNGAAVMG 184
A++ + + + + +V LG +GG F AA +
Sbjct: 112 TGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALN 170

Query: 185 VLCTLWVLKALP-SLPGESASQQQNMFGLLK 214
L L LP S GE ++ L
Sbjct: 171 GLNFLTGCFLLPESHKGERRPLRREALNPLA 201


92KPHS_52110KPHS_52020N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_52110-1214.482745putative general substrate transporter
KPHS_52100-2203.742880multidrug efflux transport outer membrane
KPHS_52090-2203.286118multidrug efflux permease EefB
KPHS_52080-1180.557979putative secretion protein
KPHS_520702190.702192hypothetical protein
KPHS_520602192.831771hypothetical protein
KPHS_520503193.592142putative acetyltransferase
KPHS_520402183.947597hypothetical protein
KPHS_520302183.737828putative TetR family transcriptional regulator
KPHS_520203184.598279multidrug resistance protein membrane
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52110TCRTETA694e-15 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 69.5 bits (170), Expect = 4e-15
Identities = 80/339 (23%), Positives = 132/339 (38%), Gaps = 25/339 (7%)

Query: 27 LPALPEITQQLQATSTQTQLSLTAALIGLGLGQLFFGP----LSDRIGRLKPLALSLLLF 82
+P LP + + L S L L Q P LSDR GR L +SL
Sbjct: 25 MPVLPGLLRDLVH-SNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGA 83

Query: 83 IFSSAMCALTRDINMLIVWRFLQGFAGAGGSVLSRSIARDKYQGTLLTQFFALLMTVNGI 142
A+ A + +L + R + G GA G+V IA D G + F + G
Sbjct: 84 AVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA-DITDGDERARHFGFMSACFGF 142

Query: 143 APVLSPVLGGYVITAFDWRILFWTMAAIGGVLLVMSLAILRETRPATAAHASRQRPGQPV 202
V PVLGG + F F+ AA+ G+ + +L E+ R+
Sbjct: 143 GMVAGPVLGGL-MGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNP-- 199

Query: 203 LKNRRFLRFCLIQAFMMA-----GLFSYIGSSSFVMQSE--YGMSAMQFSLLFGLNGI-G 254
L + R+ R + A +MA L + ++ +V+ E + A + GI
Sbjct: 200 LASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILH 259

Query: 255 LIIAAMIFSRLARRFSAESLLRGGLTLAVSCAAIMLLFA---WLHLPVLALVGL--FFTV 309
+ AMI +A R L G+ +A I+L FA W+ P++ L+
Sbjct: 260 SLAQAMITGPVAARLGERRALMLGM-IADGTGYILLAFATRGWMAFPIMVLLASGGIGMP 318

Query: 310 SLMSGISTVAGAEAMSAVDAAQSG--TASALMGTLMFVF 346
+L + +S E + + + + ++++G L+F
Sbjct: 319 ALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTA 357


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52090ACRIFLAVINRP11450.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1145 bits (2963), Expect = 0.0
Identities = 583/1031 (56%), Positives = 754/1031 (73%), Gaps = 6/1031 (0%)

Query: 3 SRFFVRRPVFAWVIAILIMLAGVLAIRTLPVGQYPDVAPPAVKISATYTGASAETLENSV 62
+ FF+RRP+FAWV+AI++M+AG LAI LPV QYP +APPAV +SA Y GA A+T++++V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 63 TQVIEQQLTGLDHLLYFSSTSSSDGSVSITVTFEQGTDPDTAQVQVQNKVQQAESRLPSE 122
TQVIEQ + G+D+L+Y SSTS S GSV+IT+TF+ GTDPD AQVQVQNK+Q A LP E
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 123 VQQSGVTVEKSQSSFLLILAVYDKTNRATSSDISDWLVSNMQDPLARVEGVGSLQVFGAE 182
VQQ G++VEKS SS+L++ T DISD++ SN++D L+R+ GVG +Q+FGA+
Sbjct: 122 VQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ 181

Query: 183 YAMRVWMDPTKLASYSLMPSDVQSAIEAQNVQVSAGKIGALPSSNAQQLTATVRAQSRLQ 242
YAMR+W+D L Y L P DV + ++ QN Q++AG++G P+ QQL A++ AQ+R +
Sbjct: 182 YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 243 TPDQFKAIIVKSQADGSVVRLSDVARVEMGSEDYTATANLNGHPAAGIAVMMAPGANALD 302
P++F + ++ +DGSVVRL DVARVE+G E+Y A +NG PAAG+ + +A GANALD
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 303 TATLVKSKIAEFQRQMPQGYDIAYPKDSTEFIKISVEDVIQTLFEAIILVVCVMYLFLQN 362
TA +K+K+AE Q PQG + YP D+T F+++S+ +V++TLFEAI+LV VMYLFLQN
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 363 FRATLIPAVAVPVVLLGTFGVLALFGYSINTLTLFAMVLAIGLLVDDAIVVVENVERIMR 422
RATLIP +AVPVVLLGTF +LA FGYSINTLT+F MVLAIGLLVDDAIVVVENVER+M
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 423 DEGLPAREATEKSMGEISGALVAIALVLSAVFLPMAFFGGSTGVIYRQFSVTIISAMMLS 482
++ LP +EATEKSM +I GALV IA+VLSAVF+PMAFFGGSTG IYRQFS+TI+SAM LS
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 483 VVVALTLTPALCGALL----SHSKPHTKGFFGAFNRLWGRTEAGYQRRVLGGLRRGAVMM 538
V+VAL LTPALC LL + + GFFG FN + + Y V L +
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 539 GAYALICGAMALAMWKLPGSFLPVEDQGEIMVQYTLPAGATAVRTAEVRRQVTDWFLTKE 598
YALI M + +LP SFLP EDQG + LPAGAT RT +V QVTD++L E
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 599 KANTDVIFTVDGFSFSGSGQNAGMAFVSLKNWSQRKGDDNTAQAIALRATKELGTIRDAT 658
KAN + +FTV+GFSFSG QNAGMAFVSLK W +R GD+N+A+A+ RA ELG IRD
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 659 LFAMTPPSVDGLGQSNGFTFELMASGGTDRDSLMKLRSQLLAAANQS-SELQSVRANDLP 717
+ P++ LG + GF FEL+ G D+L + R+QLL A Q + L SVR N L
Sbjct: 662 VIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLE 721

Query: 718 QMPQLQVDIDNNKAVSLGLSLSDVTDTLSSAWGGTYVNDFIDRGRVKKVYIQGESDARAV 777
Q ++++D KA +LG+SLSD+ T+S+A GGTYVNDFIDRGRVKK+Y+Q ++ R +
Sbjct: 722 DTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRML 781

Query: 778 PSDLGKWFVRGSDNSMTPFSAFATTHWQYGPESLVRYNGSAAFEIQGENAAGFSSGAAMD 837
P D+ K +VR ++ M PFSAF T+HW YG L RYNG + EIQGE A G SSG AM
Sbjct: 782 PEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMA 841

Query: 838 KMEKLADSLPAGSTWAWSGISLQEKLASGQAMSLYAISILVVFLCLAALYESWSVPFSVI 897
ME LA LPAG + W+G+S QE+L+ QA +L AIS +VVFLCLAALYESWS+P SV+
Sbjct: 842 LMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVM 901

Query: 898 MVIPLGLLGAALAATLRGLSNDVYFQVALLTTIGLSSKNAILIVEFAESAVD-EGYSLSR 956
+V+PLG++G LAATL NDVYF V LLTTIGLS+KNAILIVEFA+ ++ EG +
Sbjct: 902 LVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVE 961

Query: 957 AAIRAAQTRLRPIVMTSLAFIAGVLPLAIATGAGANSRVAIGTGIIGGTLTATLLAVFFV 1016
A + A + RLRPI+MTSLAFI GVLPLAI+ GAG+ ++ A+G G++GG ++ATLLA+FFV
Sbjct: 962 ATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFV 1021

Query: 1017 PLFFVLVKRLF 1027
P+FFV+++R F
Sbjct: 1022 PVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52080RTXTOXIND422e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 42.1 bits (99), Expect = 2e-06
Identities = 24/133 (18%), Positives = 51/133 (38%), Gaps = 10/133 (7%)

Query: 8 PVPVVSQLTGRTTAS-LSAEVRPQVGGIIQKRLFTEGDMVKAGQALYQIDPSSYRATWNE 66
V +V+ G+ T S S E++P I+++ + EG+ V+ G L ++ A +
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 67 AAAALKQAQALVASDCQKAQRYASLVRDNGVSRQDADDAASTCAQDKASV--------ES 118
++L QA+ Q R L + + D + ++ + +
Sbjct: 139 TQSSLLQARLEQTRY-QILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFST 197

Query: 119 KKAALESARINLN 131
+ +NL+
Sbjct: 198 WQNQKYQKELNLD 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52050SACTRNSFRASE361e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.5 bits (84), Expect = 1e-05
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 6/72 (8%)

Query: 51 GLIAKRKGNW---LCIEYLWVSETTRGRGLGSELMQEAEQQAQAQGCSHLLVDTFSFQ-- 105
G I R NW IE + V++ R +G+G+ L+ +A + A+ L+++T
Sbjct: 78 GRIKIRS-NWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINIS 136

Query: 106 ALPFYQKLGYQL 117
A FY K + +
Sbjct: 137 ACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52030HTHTETR734e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 73.1 bits (179), Expect = 4e-18
Identities = 33/175 (18%), Positives = 70/175 (40%), Gaps = 10/175 (5%)

Query: 12 RPGRPRGKKPGTANREQLMDIALTLFARDGAGRVSLNAIAKEAGVTPAMLHYYFSSRDAL 71
R + ++ R+ ++D+AL LF++ G SL IAK AGVT ++++F + L
Sbjct: 3 RKTKQEAQE----TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDL 58

Query: 72 VTQLIEERFMPLRNHISRIFVDHPQDPVL----ALTMMVETLGHMAEKNAWFAPLWM-QE 126
+++ E + P DP+ L ++E+ + ++ E
Sbjct: 59 FSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCE 118

Query: 127 IIGEMPILRQHMDARFGEERFQVMLGTVRRWQQEGKINPALAPELLFTTVISLVL 181
+GEM +++Q E + + T++ + + L + +
Sbjct: 119 FVGEMAVVQQ-AQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYIS 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_52020TCRTETB1384e-38 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 138 bits (350), Expect = 4e-38
Identities = 94/418 (22%), Positives = 178/418 (42%), Gaps = 19/418 (4%)

Query: 20 LLLVMLLSALDQTIVSTALPTIVGELGGL-DKLSWVVTAYILSSTIAVPLYGKFGDLFGR 78
L ++ S L++ +++ +LP I + +WV TA++L+ +I +YGK D G
Sbjct: 19 LCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGI 78

Query: 79 KIVLQVAIGLFLVGSALCGLAQNMTQLVLM-RGLQGLGGGGLMVISMAAVADVIPPANRG 137
K +L I + GS + + + L++M R +QG G + M VA IP NRG
Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138

Query: 138 RYQGLFGGVFGLATVIGPLIGGFLVQHASWRWIFYINLPLGLFALLVIGAVFHSSNKRSQ 197
+ GL G + + +GP IGG + + W ++ +P+ + R +
Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPMITIITVPFLMKLLKKEVRIK 196

Query: 198 HQIDWLGAIYLSMALLCIILFTSEGGSVHAWNDPQLWCILAFGIVGIIGFIYEERMAAEP 257
D G I +S+ ++ +LFT+ L ++ + F+ R +P
Sbjct: 197 GHFDIKGIILMSVGIVFFMLFTTS----------YSISFLIVSVLSFLIFVKHIRKVTDP 246

Query: 258 IIPLALFRNRSFLLCSLIGFVIGMSLFGSVTFLPLYLQVVKEATPTEAGLQLI-PLMGGL 316
+ L +N F++ L G +I ++ G V+ +P ++ V + + E G +I P +
Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSV 306

Query: 317 LLTSIISGRIISRTGKYRLFPILGTLLGVTGMVLLTRITIHSPLWQLYLFTGVLGAGLGL 376
++ I G ++ R G +G + + + + + + VLG GL
Sbjct: 307 IIFGYIGGILVDRRGP-LYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSF 364

Query: 377 VMQVLVLAVQNAMPAQMYGVATSGATLFRSIGGSIGVALFGAVFTHVLQSNLQQLLPE 434
V+ V +++ Q G S + G+A+ G + + + Q+LLP
Sbjct: 365 TKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS--IPLLDQRLLPM 420


93KPHS_50680KPHS_50620N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_50680-1101.918052putative outer membrane lipoprotein
KPHS_50670-1100.991216biotin sulfoxide reductase
KPHS_50660-1130.244354GNAT family acetyltransferase
KPHS_50650-213-0.7402263-methyladenine DNA glycosylase
KPHS_50640-214-1.439361putative lipase
KPHS_50630012-2.240121MFS superfamily oxalate/formate antiporter
KPHS_50620011-2.062266putative transmembrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50680OMPADOMAIN1186e-34 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 118 bits (296), Expect = 6e-34
Identities = 43/124 (34%), Positives = 65/124 (52%), Gaps = 11/124 (8%)

Query: 108 LNMPNNVTFDSNSANLKPAGANTLTGVAMVLKEYEKT--AVNVVGYTDSTGSKDLNMRLS 165
+ ++V F+ N A LKP G L + L + +V V+GYTD GS N LS
Sbjct: 215 FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS 274

Query: 166 QQRADSVASALITQGVAANRIRTTGMGPANPIASNSTAEGK---------AQNRRVEITL 216
++RA SV LI++G+ A++I GMG +NP+ N+ K A +RRVEI +
Sbjct: 275 ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 217 SPLQ 220
++
Sbjct: 335 KGIK 338


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50660SACTRNSFRASE331e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 33.4 bits (76), Expect = 1e-04
Identities = 18/52 (34%), Positives = 24/52 (46%), Gaps = 5/52 (9%)

Query: 76 VAPGATRQGIGRALLDEIKQ-----HYAWLSLEVYQKNESAVSFYHAQGFRI 122
VA ++G+G ALL + + H+ L LE N SA FY F I
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50630TCRTETA414e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 41.3 bits (97), Expect = 4e-06
Identities = 48/276 (17%), Positives = 92/276 (33%), Gaps = 33/276 (11%)

Query: 44 PVSQVAFSFGLLSLGLALS----SSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSSSL 99
+ V +G+L AL + V G L +RFG + V + S + + + A + L
Sbjct: 37 HSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFL 96

Query: 100 MMLWLS---AGVLVGLADGAGYLL----TLSNCVKWFPERKGLISAFSIGSYGLGSLGFK 152
+L++ AG+ AG + + F + LG
Sbjct: 97 WVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGG---- 152

Query: 153 FIDSHLLATVGLEKTFVIWGAIVLVMIVFGATLMKDAPNHPAATAANGVVENDFTLAESM 212
L+ F A+ + + G L+ + + N
Sbjct: 153 -----LMGGFSPHAPFFAAAALNGLNFLTGCFLLPE-SHKGERRPLRREALNPLASFRWA 206

Query: 213 R--KPQYWMLAVMFLTACMSG----LYVIGVAKDIAQGMVHLDVATAANAVTVISIAN-L 265
R ++AV F+ + L+VI + H D T ++ I + L
Sbjct: 207 RGMTVVAALMAVFFIMQLVGQVPAALWVI-----FGEDRFHWDATTIGISLAAFGILHSL 261

Query: 266 SGRLVLGILSDKISRIRVITIGQVVSLVGMAALLFA 301
+ ++ G ++ ++ R + +G + G L FA
Sbjct: 262 AQAMITGPVAARLGERRALMLGMIADGTGYILLAFA 297



Score = 34.0 bits (78), Expect = 9e-04
Identities = 31/119 (26%), Positives = 51/119 (42%), Gaps = 2/119 (1%)

Query: 270 VLGILSDKISRIRVITIGQVVSLVGMAALLFAPLNALTFFAAIACVAFNFGGTITVFPSL 329
VLG LSD+ R V+ + + V A + AP + + I VA G T V +
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRI--VAGITGATGAVAGAY 119

Query: 330 VSEFFGLNNLAKNYGVIYLGFGIGSICGSLIASLFGGFYVTFCVIFALLILSLALSTTI 388
+++ + A+++G + FG G + G ++ L GGF A + L T
Sbjct: 120 IADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGC 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50620PF06580290.049 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.1 bits (65), Expect = 0.049
Identities = 23/137 (16%), Positives = 43/137 (31%), Gaps = 23/137 (16%)

Query: 5 RTMTQQKLSFWLALYIGWFMNVAVFFRRFDGYAQEFTFWKGLSGVVELVATVFVTFFLLR 64
T Q +W IGW + F G+A + K S + + ++
Sbjct: 3 STHRQANKYYWYCQGIGWGVYTLTGF----GFASLYGSPKLHSMIFNIAISLMGLVLTHA 58

Query: 65 LLSLFGRRIWRILATLIVLFSAAASYYMTFLNVVIGYGIIASVMTTDIDLSKEVIGWHLI 124
S R+ W L ++ + + G++ V T I W L+
Sbjct: 59 YRSFIKRQGWLKLNMGQIILRVLPA--------CVVIGMVWFVANTSI--------WRLL 102

Query: 125 LWLVAVSAPPLLFIWSN 141
++ + P+ F
Sbjct: 103 AFI---NTKPVAFTLPL 116


94KPHS_50310KPHS_50260N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_503100152.339292antibiotic biosynthesis monooxygenase
KPHS_50300-1163.336500hypothetical protein
KPHS_50290-1163.195540metabolite/H+ symporter, major facilitator
KPHS_502800154.200717hypothetical protein
KPHS_502700164.215866putative LysR-family transcriptional regulator
KPHS_50260-2163.778847oxidoreductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50310FLGMOTORFLIG270.004 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 27.1 bits (60), Expect = 0.004
Identities = 10/47 (21%), Positives = 19/47 (40%), Gaps = 5/47 (10%)

Query: 1 MHHVSPEYSQKAAFILEMRFSSHYARQVKK-----IMERCFNMIDQQ 42
M SPE ++ +LE + +S + + NM D++
Sbjct: 174 MDRTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRK 220


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50290TCRTETB310.008 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.4 bits (71), Expect = 0.008
Identities = 79/365 (21%), Positives = 126/365 (34%), Gaps = 59/365 (16%)

Query: 79 IGSALFGHFGDRVGRKVTLVASLLTMGISTVVIGLLPGYEIIGIVAPMLLALARFGQGLG 138
IG+A++G D++G K LL GI G + G+ +G LL +ARF QG G
Sbjct: 64 IGTAVYGKLSDQLGIK-----RLLLFGIIINCFGSVIGF--VGHSFFSLLIMARFIQGAG 116

Query: 139 LGGEWGGAALLATENAPARKR----ALYGSFPQLGAPIGFFFANGTFLLLSW-------- 186
++ P R L GS +G +G + W
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM 176

Query: 187 -----LLTDQQFMEWGWRV--PF-IFSAVLVIIG-------------LYVRVSLHETPVF 225
+ + ++ R+ F I +L+ +G ++ VS+ +F
Sbjct: 177 ITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIF 236

Query: 226 AKVAAAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFSTGAAPNGLGL 285
K + G + VL I+ T F M Y M + +G
Sbjct: 237 VKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIG- 295

Query: 286 PRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMII-ITTMIILFALFAFKPLLGSGN 344
+ +++ M+VI FG + G+L D G + I +T + + F +F S
Sbjct: 296 --SVIIFPGTMSVIIFG---YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWF 350

Query: 345 PLLVFAFLLLGLSLMGL---TFGPMGALLPELFPTEVRYTGASFS-YNVSSILGASVAPY 400
++ F+L GLS T L E GA S N +S L
Sbjct: 351 MTIIIVFVLGGLSFTKTVISTIVSSS-----LKQQEA---GAGMSLLNFTSFLSEGTGIA 402

Query: 401 IAAWL 405
I L
Sbjct: 403 IVGGL 407



Score = 29.5 bits (66), Expect = 0.026
Identities = 19/101 (18%), Positives = 38/101 (37%), Gaps = 2/101 (1%)

Query: 255 FIMLATYTLFYIMTVYSMTFSTGAAPNGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADA 314
I L + F ++ + S N P W+ ++ F + V G L+D
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 315 FGRRKSMIIITTMIILFALFAFKPLLGSGNPLLVFAFLLLG 355
G ++ ++ + ++ F + S LL+ A + G
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGF--VGHSFFSLLIMARFIQG 114


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50280DPTHRIATOXIN300.015 Diphtheria toxin signature.
		>DPTHRIATOXIN#Diphtheria toxin signature.

Length = 567

Score = 30.1 bits (67), Expect = 0.015
Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 6/40 (15%)

Query: 19 EKSKSTLEALNDTAVGQKASQALKTVTGTAAKVQRNPVIA 58
EK+K LE + TA+ LKTVTGT NPV A
Sbjct: 273 EKAKQYLEEFHQTALEHPELSELKTVTGT------NPVFA 306


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_50260DHBDHDRGNASE857e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 85.1 bits (210), Expect = 7e-22
Identities = 67/258 (25%), Positives = 113/258 (43%), Gaps = 16/258 (6%)

Query: 4 RIALVTGGSRGLGKNAALKLAAKGTDILLTYHSNRQAALDVVAEIEQKGVKAAALALNVG 63
+IA +TG ++G+G+ A LA++G I N + VV+ ++ + A A +V
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 64 DSTTFDAFASEVAQVLAQKWGRTTFDYLLNNAGIGLNAPFAETSEAQFDELMNIQFKGPF 123
DS D E+ + ++ G D L+N AG+ S+ +++ ++ G F
Sbjct: 68 DSAAID----EITARIEREMGP--IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVF 121

Query: 124 FLTQRLLPLLQD--GGRILNVSSGLARFALPGYAAYAAMKGAMEVLTRYQAKELGGRGIS 181
++ + + D G I+ V S A AAYA+ K A + T+ EL I
Sbjct: 122 NASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181

Query: 182 VNIIAPGAIETDFGGG-EVRDNAE--VNRHIAAQTALG----RVGLPDDIGDAIAALLSD 234
NI++PG+ ETD +N V + G ++ P DI DA+ L+S
Sbjct: 182 CNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 235 ELAWMNAQRVEVSGGMFL 252
+ + + V GG L
Sbjct: 242 QAGHITMHNLCVDGGATL 259


95KPHS_49660KPHS_49590N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_49660-3150.548902glycerol-3-phosphate transporter periplasmic
KPHS_49650-1130.960603glycerol-3-phosphate transporter permease
KPHS_496400140.959267sn-glycerol 3-phosphate transport membrane
KPHS_49630-1131.114853glycerol-3-phosphate transporter ATP-binding
KPHS_49620-3130.082584glycerophosphodiester phosphodiesterase
KPHS_49610-313-0.640103hypothetical protein
KPHS_49600-111-0.308795gamma-glutamyltranspeptidase
KPHS_49590-113-1.047069putative acyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49660MALTOSEBP386e-05 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 38.2 bits (88), Expect = 6e-05
Identities = 44/175 (25%), Positives = 73/175 (41%), Gaps = 17/175 (9%)

Query: 134 GHLLSQPFNSSTPVLYYNKDAFKKAGLDPDQPPKTWQDLAAYTAKLKAAGMKCGYASGWQ 193
G L++ P L YNKD L P+ PPKTW+++ A +LKA G + +
Sbjct: 127 GKLIAYPIAVEALSLIYNKD------LLPN-PPKTWEEIPALDKELKAKGKSALMFNLQE 179

Query: 194 GWIQIENFSAWHGLPVATKNNGFDGTDAVLEF--NKPEQVKHIALLEEMNKKGDFSYFGR 251
+ +A G +N +D D ++ K + L++ + D Y
Sbjct: 180 PYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDY--- 236

Query: 252 KDESTEKFYNGDCAITTASSGSLADIRQYAKFNYGVGMMPYDADVKGAPQNAIIG 306
+ F G+ A+T + ++I +K NYGV ++P KG P +G
Sbjct: 237 -SIAEAAFNKGETAMTINGPWAWSNIDT-SKVNYGVTVLP---TFKGQPSKPFVG 286


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49630PF05272290.037 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.9 bits (64), Expect = 0.037
Identities = 10/29 (34%), Positives = 16/29 (55%)

Query: 33 IVMVGPSGCGKSTLLRMVAGLERVTSGDI 61
+V+ G G GKSTL+ + GL+ +
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHF 627


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49620PF04619280.017 Dr-family adhesin
		>PF04619#Dr-family adhesin

Length = 160

Score = 28.4 bits (63), Expect = 0.017
Identities = 14/60 (23%), Positives = 23/60 (38%), Gaps = 4/60 (6%)

Query: 29 VGARYGHTMIEFDAKLSKDGQIFLLHDDNLERTSNGWGVAGELAW----DDLLKVDAGSW 84
+G ++ D + G+ FL+ D+N ++ AW K D GSW
Sbjct: 70 LGCDARQVALKADTDNFEQGKFFLISDNNRDKLYVNIRPTDNSAWTTDNGVFYKNDVGSW 129


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49600NAFLGMOTY330.003 Sodium-type flagellar protein MotY precursor signature.
		>NAFLGMOTY#Sodium-type flagellar protein MotY precursor signature.

Length = 293

Score = 33.2 bits (75), Expect = 0.003
Identities = 27/80 (33%), Positives = 36/80 (45%), Gaps = 13/80 (16%)

Query: 276 RTPVSGEYRGYEVYSMPPPSSGGIHIVQILNILENFDMQKYGF-GSADAMQVMAEAEKHA 334
R P+ GE R + SMPPP G H +I N+ F Q G+ G A +++E EK
Sbjct: 77 RRPM-GETRNVSLISMPPPWRPGEHADRITNL--KFFKQFDGYVGGQTAWGILSELEKGR 133

Query: 335 YADRSEYLGDPDFVNVPWQA 354
Y P F WQ+
Sbjct: 134 Y---------PTFSYQDWQS 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49590SACTRNSFRASE371e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.8 bits (85), Expect = 1e-05
Identities = 17/63 (26%), Positives = 27/63 (42%), Gaps = 13/63 (20%)

Query: 80 MAVAAGHQGCGIGSALMREMID------LCDNWLRVERIELTVFADNAPAIAVYKKYGFE 133
+AVA ++ G+G+AL+ + I+ C L + I N A Y K+ F
Sbjct: 95 IAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDI-------NISACHFYAKHHFI 147

Query: 134 IEG 136
I
Sbjct: 148 IGA 150


96KPHS_49160KPHS_49110N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_491601162.112020hypothetical protein
KPHS_491501160.218296hypothetical protein
KPHS_49140016-0.735594outer membrane porin
KPHS_49130119-1.459068shikimate kinase I
KPHS_49120116-1.4181903-dehydroquinate synthase
KPHS_49110014-0.527366hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49160PYOCINKILLER335e-04 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 32.8 bits (74), Expect = 5e-04
Identities = 28/118 (23%), Positives = 43/118 (36%), Gaps = 16/118 (13%)

Query: 37 LLVSRTARLQRDFLATLHTTADAQLLASLKQREQAMREAWQQHQRQRQQYQRRSAIAAWQ 96
L + LQ A + A+ K REQA EA +++ + Q R A
Sbjct: 192 LFTEAISSLQIRMNTLTAAKASIEAAAANKAREQAAAEA-----KRKAEEQARQQAAIRA 246

Query: 97 PRLQVLAAD----LPAQAWLTRLEYQGVLLTLDGLALNLQALTSVEAALTRVAGFAPA 150
+ A+ A +G++ G A QA++ A L RV AP+
Sbjct: 247 ANTYAMPANGSVVATAAG-------RGLIQVAQGAASLAQAISDAIAVLGRVLASAPS 297


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49140TYPE3OMGPROT2263e-70 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 226 bits (578), Expect = 3e-70
Identities = 77/282 (27%), Positives = 122/282 (43%), Gaps = 17/282 (6%)

Query: 138 GGKLLSARGHLMADKRTNRLLIRDDARHLPALKAWAQEMDLPVGQVELAAHIVSMSETSL 197
SA+ + AD N +++RD +P + +D P ++E+A IV ++ L
Sbjct: 237 AATRASAQARVEADPSLNAIIVRDSPERMPMYQRLIHALDKPSARIEVALSIVDINADQL 296

Query: 198 RELGVKWRLAEAGSPPGSGQITTLSSDVSVNDASTRAGFNIGKINGRLLEL---ELSALE 254
ELGV WR+ I T ++ G ++ R L+ ++ LE
Sbjct: 297 TELGVDWRVGIRTGNNHQVVIKTTGDQSNIAS----NGALGSLVDARGLDYLLARVNLLE 352

Query: 255 RKQQVEIIASPRLLASHMQPASIKQGSEIPYQVSSGESGATSVEFKEAVLG--MEVTPTV 312
+ ++++ P LL A I SE Y +G+ A E K G + +TP V
Sbjct: 353 NEGSAQVVSRPTLLTQENAQAVIDH-SETYYVKVTGKEVA---ELKGITYGTMLRMTPRV 408

Query: 313 LQQG---RVRLKLRISENTPGQVLKQENGEALAIDKQEIETLVEVRSGETLALGGIFSQK 369
L QG + L L I + I + ++T+ V G++L +GGI+ +
Sbjct: 409 LTQGDKSEISLNLHIEDGNQKPNS-SGIEGIPTISRTVVDTVARVGHGQSLIIGGIYRDE 467

Query: 370 NKTARDSVPLLGDIPVLGRLFRRDGKDNERRELVVFITPRIL 411
A VPLLGDIP +G LFRR + R + I PRI+
Sbjct: 468 LSVALSKVPLLGDIPYIGALFRRKSELTRRTVRLFIIEPRII 509


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49130CARBMTKINASE310.002 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 30.6 bits (69), Expect = 0.002
Identities = 27/91 (29%), Positives = 40/91 (43%), Gaps = 18/91 (19%)

Query: 20 FYDSDQEIEKRTGADVGWVFDVEGEEGFRD----------REEKIINELTEKQGIVLATG 69
FYD + KR + GW+ + G+R E + I +L E+ IV+A+G
Sbjct: 136 FYDEETA--KRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETIKKLVERGVIVIASG 193

Query: 70 GGSVKSRETRNRLSARGVVVYLETTIEKQLA 100
GG V + +GV E I+K LA
Sbjct: 194 GGGVPVILEDGEI--KGV----EAVIDKDLA 218


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_49110IGASERPTASE356e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 35.0 bits (80), Expect = 6e-04
Identities = 38/211 (18%), Positives = 63/211 (29%), Gaps = 18/211 (8%)

Query: 125 SSSQQTASGEKSINLSDDQSASMPAAGQDQTAAANSTSQQDVTVPPIAANPTQGQAAAAP 184
S + A + +T A NS + Q A
Sbjct: 1009 SVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTV-------EKNEQDATET 1061

Query: 185 QGQQRIEVQGDLNN--ALTQQ----QGQLDGAVANSTLPTEPATVAPIRNGANGTAAPRQ 238
Q R + +N A TQ Q + +T E ATV T ++
Sbjct: 1062 TAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQE 1121

Query: 239 ATERQTAATPRPAERKHTVIEAKPQSKPQAVAKTPVESKPVQPKHVESTATTAPAKTSVS 298
+ + +P+ + + +PQ++P V K Q + + T PAK + S
Sbjct: 1122 VPKVTSQVSPKQEQSE----TVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSS 1177

Query: 299 ESKPVATAQSKPTTTTAAPAATAAAAAPAAK 329
+ T +S T + PA
Sbjct: 1178 NVEQPVT-ESTTVNTGNSVVENPENTTPATT 1207


97KPHS_48750KPHS_48710N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_48750549-6.117474elongation factor G
KPHS_48740237-6.205375elongation factor Tu
KPHS_48730132-6.759998bacterioferritin-associated ferredoxin
KPHS_48720232-5.845391bacterioferritin
KPHS_48710640-4.989385leader peptidase HopD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48750TCRTETOQM6130.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 613 bits (1583), Expect = 0.0
Identities = 179/698 (25%), Positives = 304/698 (43%), Gaps = 81/698 (11%)

Query: 9 RYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAAT 68
+ NIG+ AH+DAGKTT TE +L+ +G ++G V G D E++RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 69 TAFWSGMAKQYEPHRVNIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWR 128
+ W +VNIIDTPGH+DF EV RS+ VLDGA+++ A GVQ Q+ ++
Sbjct: 62 SFQWEN-------TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFH 114

Query: 129 QANKYKVPRIAFVNKMDRMGANFLKVVGQIKTRLGANPVPLQLAIGAEEGFTGVVDLVKM 188
K +P I F+NK+D+ G + V IK +L A V Q V M
Sbjct: 115 ALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ----------KVELYPNM 164

Query: 189 KAINWNDADQGVTFEYEDIPADMQDLADEWHQNLIESAAEASEELMEKYLGGEELTEEEI 248
N+ +++Q ++ E +++L+EKY+ G+ L E+
Sbjct: 165 CVTNFTESEQ------------------------WDTVIEGNDDLLEKYMSGKSLEALEL 200

Query: 249 KKALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDDGKDTPAE 308
++ R N + V GSA N G+ +++ + + S
Sbjct: 201 EQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH----------------- 243

Query: 309 RHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHA 368
FKI L + R+YSGV++ D+V S K + + +
Sbjct: 244 ---RGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEK-EKIKITEMYTSIN 299

Query: 369 NKREEIKEVRAGDIAAAIG----LKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKT 424
+ +I + +G+I L V GDT P ER+E P P++ VEP
Sbjct: 300 GELCKIDKAYSGEIVILQNEFLKLNSV-LGDTKLLPQR----ERIENPLPLLQTTVEPSK 354

Query: 425 KADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVEANVG 484
+E + AL ++ DP R + D +++ I++ +G++ +++ ++ +++VE +
Sbjct: 355 PQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIK 414

Query: 485 KPQVAYREAIRAKVTDIEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGV 544
+P V Y E K E + + + + + PL GS G ++ + + G
Sbjct: 415 EPTVIYMERPLKKA---EYTIHIEVPPNPFWASIGLSVSPLPLGS---GMQYESSVSLGY 468

Query: 545 IPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEG 604
+ + AV +GI+ + G L G+ V D I +G Y+ S+ F++ A I ++
Sbjct: 469 LNQSFQNAVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQV 527

Query: 605 FKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLRGQESEVTGVKIHAEVPLSEMF 664
KKA LLEP + ++ P+E D + + + + V + E+P +
Sbjct: 528 LKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQ 587

Query: 665 GYATQLRSLTKGRASYTMEFLKYDDAPNNVAQAVIEAR 702
Y + L T GR+ E Y + V + R
Sbjct: 588 EYRSDLTFFTNGRSVCLTELKGYHVT---TGEPVCQPR 622


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48740TCRTETOQM804e-18 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 79.5 bits (196), Expect = 4e-18
Identities = 53/154 (34%), Positives = 78/154 (50%), Gaps = 13/154 (8%)

Query: 13 VNVGTIGHVDHGKTTLTAAI------TTVLAKTYGGSARAFDQIDNAPEEKARGITINTS 66
+N+G + HVD GKTTLT ++ T L G+ R DN E+ RGITI T
Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRT----DNTLLERQRGITIQTG 59

Query: 67 HVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQV 126
+ +D PGH D++ + + +DGAIL+++A DG QTR R++
Sbjct: 60 ITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKM 119

Query: 127 GVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ 160
G+P I F+NK D + L V +++E LS
Sbjct: 120 GIP-TIFFINKIDQNGID--LSTVYQDIKEKLSA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48720HELNAPAPROT379e-06 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 36.8 bits (85), Expect = 9e-06
Identities = 18/103 (17%), Positives = 42/103 (40%), Gaps = 10/103 (9%)

Query: 44 EYHESIDEMKHADKYIERILFLEGIPN--LQDLGKL------GIGEDVEEMLRSDLRLEL 95
E ++ E D ER+L + G P +++ + G EM+++ +
Sbjct: 52 ELYDHAAE--TVDTIAERLLAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYK 109

Query: 96 EGAQNLREAIAYADSVHDYVSRDMMIEILADEEGHIDWLETEL 138
+ + + I A+ D + D+ + ++ + E + L + L
Sbjct: 110 QISSESKFVIGLAEENQDNATADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48710PREPILNPTASE1225e-37 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 122 bits (308), Expect = 5e-37
Identities = 67/150 (44%), Positives = 85/150 (56%), Gaps = 7/150 (4%)

Query: 2 LAALPFLLCYSGLTVALCHQDLRHGLLPDRYTCPLLWSGLLFYLCLAPHQLHDAVWGAIT 61
L LL L VAL DL LLPD+ T PLLW GLLF L L DAV GA+
Sbjct: 132 WGTLAALLLTWVL-VALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMA 190

Query: 62 GYLSLAAIYWLYRGIRGYEGLGYGDIKYLAALGAWHGWRLLPQLVLVASLLAGIAWAGAG 121
GYL L ++YW ++ + G EG+GYGD K LAALGAW GW+ LP ++L++SL+
Sbjct: 191 GYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFM---GI 247

Query: 122 LYASCGRKSKWGRSNPLPFGPFLAAAGFWC 151
+S P+PFGP+LA AG+
Sbjct: 248 GLILLRNHH---QSKPIPFGPYLAIAGWIA 274


98KPHS_48280KPHS_48250N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_48280-125-6.130797*hypothetical protein
KPHS_48270-126-6.390130inner membrane multidrug efflux protein BpeB
KPHS_48260239-8.802351transmembrane protein
KPHS_48250346-10.662717DNA-binding transcriptional regulator EnvR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48280PF06291270.005 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 26.5 bits (58), Expect = 0.005
Identities = 20/65 (30%), Positives = 27/65 (41%), Gaps = 3/65 (4%)

Query: 1 MKKYLIVALLASLLAGCAHDSPCV---PVYDSQGRLVHTNTCMKGTTEDNWETAGAIAGG 57
MKK L A LA L+ GCA + V P + + + + G + A I GG
Sbjct: 6 MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVSGIGQKKTVDAAKICGG 65

Query: 58 AAAVA 62
A V
Sbjct: 66 AENVV 70


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48270ACRIFLAVINRP13720.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1372 bits (3554), Expect = 0.0
Identities = 892/1032 (86%), Positives = 958/1032 (92%), Gaps = 1/1032 (0%)

Query: 1 MSKFFIHRPVFAWVLAIIMMIAGGLAILQLPIAQYPTIAPPAVAISATYPGADAQTVQDT 60
M+ FFI RP+FAWVLAII+M+AG LAILQLP+AQYPTIAPPAV++SA YPGADAQTVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFKSGTDPDIAQVQVQNKLQLATPLLPQ 120
VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTF+SGTDPDIAQVQVQNKLQLATPLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGISVEKSSSSFLLVAGFISDNPTTTQDDISDYVASNVKDPISRLNGVGDVQLFGA 180
EVQQQGISVEKSSSS+L+VAGF+SDNP TTQDDISDYVASNVKD +SRLNGVGDVQLFGA
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRVWLDGNLLNKYNLTPVDVINALQVQNDQIAAGQLGGTPALKGQQLNASIIAQTRL 240
QYAMR+WLD +LLNKY LTPVDVIN L+VQNDQIAAGQLGGTPAL GQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 KDPQEFGKVTLRVNADGSVVHLKDVARIELGGENYNVVARINGKPASGLGIKLATGANAL 300
K+P+EFGKVTLRVN+DGSVV LKDVAR+ELGGENYNV+ARINGKPA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTATAIKAKLAELQPYFPQGMKVVYPYDTTPFVKISIHEVVKTLFEAIILVFLVMYLFLQ 360
DTA AIKAKLAELQP+FPQGMKV+YPYDTTPFV++SIHEVVKTLFEAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NMRATLIPTIAVPVVLLGTFAVLSMFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
NMRATLIPTIAVPVVLLGTFA+L+ FGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 VEEKLSPKEATEKSMSQIQGALVGIAMVLSAVFVPMAFFGGSTGAIYRQFSITIVSAMAL 480
+E+KL PKEATEKSMSQIQGALVGIAMVLSAVF+PMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALVLTPALCATLLKPASAEHHE-KKGFFGWFNARFDQSVNHYTNSVSGILRGTGRY 539
SVLVAL+LTPALCATLLKP SAEHHE K GFFGWFN FD SVNHYTNSV IL TGRY
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 540 LVIYLLIVVGMAVLFMRLHTSFLPDEDQGVFLTMIQLPSGATQERTQKVLDTVTDYYLHN 599
L+IY LIV GM VLF+RL +SFLP+EDQGVFLTMIQLP+GATQERTQKVLD VTDYYL N
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 600 EKANVESVFTVNGFSFSGQGQNSGMAFVSLKPWEARSGDENSVESIIKRATVAFSQIKDA 659
EKANVESVFTVNGFSFSGQ QN+GMAFVSLKPWE R+GDENS E++I RA + +I+D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 660 MVFPFNMPAIIELGTATGFDFELIDQGGLGHTALTQARNQLLGMVKQHPDQLVRVRPNGL 719
V PFNMPAI+ELGTATGFDFELIDQ GLGH ALTQARNQLLGM QHP LV VRPNGL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 720 EDTPQFKLDVDQEKAQALGVSLSDINETISAALGGYYVNDFIDRGRVKKVYVQADAHFRM 779
EDT QFKL+VDQEKAQALGVSLSDIN+TIS ALGG YVNDFIDRGRVKK+YVQADA FRM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 780 LPSDINNMYVRSANGEMVPFSAFVTSRWIYGSPRLERYNGLPSMEILGEASPGKSTGEAM 839
LP D++ +YVRSANGEMVPFSAF TS W+YGSPRLERYNGLPSMEI GEA+PG S+G+AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 840 ALMETLASKLPSGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFSV 899
ALME LASKLP+GIGYDWTGMSYQERLSGNQAPAL AIS +VVFLCLAALYESWSIP SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 900 MLVVPLGVIGALLAATLRGLNNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGII 959
MLVVPLG++G LLAATL NDVYF VGLLTTIGLSAKNAILIVEFAKDLMEKEGKG++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 960 EATLEASRMRLRPILMTSLAFILGVMPLVISHGAGSGAQNAVGTGVMGGMLTATLLAIFF 1019
EATL A RMRLRPILMTSLAFILGV+PL IS+GAGSGAQNAVG GVMGGM++ATLLAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1020 VPVFFVVVRRRF 1031
VPVFFVV+RR F
Sbjct: 1021 VPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48260RTXTOXIND371e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.7 bits (85), Expect = 1e-04
Identities = 34/211 (16%), Positives = 67/211 (31%), Gaps = 30/211 (14%)

Query: 97 ATYQAAWNSAKGDEAKAEAAAAIAHLTVKRYVPLLGTKYISQQEYDQAVATA-RQADADV 155
K + E+ A + Q + + RQ ++
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLV----------TQLFKNEILDKLRQTTDNI 311

Query: 156 IATKAAVETARINLAYTKVTSPISGRIGKSSV-TEGALVTNGQSDALATVQQLDPIYVDV 214
+ + + +P+S ++ + V TEG +VT ++ + V + D + V
Sbjct: 312 GLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAET-LMVIVPEDDTLEVTA 370

Query: 215 TESSNDFMRLKQESLQRGGDTKSVELVMENGQAYP-LKGSLQ--FSDVTVDESTG----- 266
+ D + G +++ Y L G ++ D D+ G
Sbjct: 371 LVQNKDIGFINV------GQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNV 424

Query: 267 --SITLRAIFPNPQHV-LLPGMFVRARIDEG 294
SI + +++ L GM V A I G
Sbjct: 425 IISIEENCLSTGNKNIPLSSGMAVTAEIKTG 455



Score = 35.2 bits (81), Expect = 3e-04
Identities = 23/127 (18%), Positives = 41/127 (32%), Gaps = 15/127 (11%)

Query: 46 APLSVTTELPGR-TSAFRVAEVRPQVSGIILKRNFV-EGSDVEAGQSLYQIDPATYQAAW 103
+ + G+ T + R E++P + I+ K V EG V G L ++ +A
Sbjct: 78 GQVEIVATANGKLTHSGRSKEIKPIENSIV-KEIIVKEGESVRKGDVLLKLTALGAEA-- 134

Query: 104 NSAKGDEAKAEAAAAIAHLTVKRYVPLLGTKYISQQEYDQAVATARQADADVIATKAAVE 163
D K +++ A L RY L E ++ +
Sbjct: 135 -----DTLKTQSSLLQARLEQTRYQILS-----RSIELNKLPELKLPDEPYFQNVSEEEV 184

Query: 164 TARINLA 170
+L
Sbjct: 185 LRLTSLI 191


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_48250HTHTETR1182e-35 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 118 bits (297), Expect = 2e-35
Identities = 77/201 (38%), Positives = 124/201 (61%), Gaps = 3/201 (1%)

Query: 1 MARKTKEEAQRTRQLLIESAIQQFALRGVTNTTLTDIADAAGVTRGAVYWHFASKTELFN 60
MARKTK+EAQ TRQ +++ A++ F+ +GV++T+L +IA AAGVTRGA+YWHF K++LF+
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EMW-QQQPPLRDLIQPSQAIEYEHEPLNALRERFIAGLRYIAANPRQRALMQILYQRCEF 119
E+W + + +L QA ++ +PL+ LRE I L R+R LM+I++ +CEF
Sbjct: 61 EIWELSESNIGELELEYQA-KFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEF 119

Query: 120 SSDMLSEYEIRQRIGF-NYSLIGGILQCCVRNNILPAETNIEMILIVLHSAFSGLIKNWL 178
+M + ++ + +Y I L+ C+ +LPA+ I++ SGL++NWL
Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179

Query: 179 LDPQRFDLYQQAPALVDNIMA 199
PQ FDL ++A V ++
Sbjct: 180 FAPQSFDLKKEARDYVAILLE 200


99KPHS_44360KPHS_44290N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_443601161.539248short chain dehydrogenase
KPHS_443501151.911394putative regulatory protein IclR
KPHS_443401132.191922putative glyoxalase/bleomycin resistance
KPHS_443301152.803974putative ferredoxin reductase subunit
KPHS_443202142.612106major facilitator superfamily transporter MFS_1
KPHS_443101143.128062putative cupin domain protein
KPHS_443002132.579737putative hydrolase
KPHS_442901131.975603short chain dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44360DHBDHDRGNASE1003e-27 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 99.7 bits (248), Expect = 3e-27
Identities = 72/256 (28%), Positives = 122/256 (47%), Gaps = 20/256 (7%)

Query: 5 LNGKRIVVTGAARGLGYHFAEACAAQGATVVMCDILQGELAESAHRLQRKGYQVESHAID 64
+ GK +TGAA+G+G A A+QGA + D +L + L+ + E+ D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 65 LASQASIEQVFSAIGAQ-GSIDGLVNNAAMATGVGGKNMIDYDPDLWDRVMTVNVKGTWL 123
+ A+I+++ + I + G ID LVN A + ++ D + W+ +VN G +
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEE---WEATFSVNSTGVFN 122

Query: 124 VTRAAVPLL--REGAAIVNVASDTALWGAPR--LMAYVASKGAVIAMTRSMARELGEKRI 179
+R+ + R +IV V S+ A G PR + AY +SK A + T+ + EL E I
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 180 RINAIAPGLTRVE----------ATEYVPAERHQLYENGRALSGAQQPEDVTGSVVWLLS 229
R N ++PG T + E V + ++ G L +P D+ +V++L+S
Sbjct: 181 RCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240

Query: 230 DLSRFITGQLIPVNGG 245
+ IT + V+GG
Sbjct: 241 GQAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44320TCRTETB424e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 41.8 bits (98), Expect = 4e-06
Identities = 26/132 (19%), Positives = 57/132 (43%), Gaps = 3/132 (2%)

Query: 75 AFLLSYGFSSVLLSGLGDRIAPLRLLTGMMVVWCILMVIMGFTHNYALMVTLRILLGIAE 134
AF+L++ + + L D++ RLL +++ C VI H++ ++ + + A
Sbjct: 57 AFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAG 116

Query: 135 GPLFPLAFAVVRHTF-PQRLQARATMLWLLGTPVGAALGFPLSIWLLNTFGWQSTFFVM- 192
FP VV + P+ + +A L +G +G + + + W +
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM 176

Query: 193 -AMLTIPVLIFV 203
++T+P L+ +
Sbjct: 177 ITIITVPFLMKL 188


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44300PF06057290.016 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 29.0 bits (65), Expect = 0.016
Identities = 21/88 (23%), Positives = 33/88 (37%), Gaps = 8/88 (9%)

Query: 28 KQMALPGYRVLAWDMPGYGESPMLAATPAD-AGDYADALARMLDRAGVEQTILVGHSLGA 86
+ G+ V+ W Y P D D + + G ++ IL+G+S GA
Sbjct: 72 GILQQQGWPVVGWSSLKYYWK---QKDPKDVTQDTLAIIDKYQAEFGTQKVILIGYSFGA 128

Query: 87 LVAAAFAAKYP----QRVLYLVLADVAQ 110
V + P + VL VL +Q
Sbjct: 129 EVIPFVLNEMPARYRKNVLGAVLLSPSQ 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_44290DHBDHDRGNASE1081e-30 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 108 bits (272), Expect = 1e-30
Identities = 77/262 (29%), Positives = 118/262 (45%), Gaps = 9/262 (3%)

Query: 1 MNAQ-IEGRVAVVTGGSSGIGFETLRLLLGEGAKVAFCGRNPDRLASAHAALQNE--YPE 57
MNA+ IEG++A +TG + GIG R L +GA +A NP++L ++L+ E + E
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 58 GEVFSWRCDVLNEAEVEAFAAAVAARFGGVDMLINNAGQGYVAHFADTPREAWLHEAELK 117
++ DV + A ++ A + G +D+L+N AG E W +
Sbjct: 61 ----AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVN 116

Query: 118 LFGVINPVKAFQSLLEASDIASITCVNSLLALQPEEHMIATSAARAALLNMTLTLSKELV 177
GV N ++ + SI V S A P M A ++++AA + T L EL
Sbjct: 117 STGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 178 DKGIRVNSILLGMVESGQWQRRFESRSDKSQSWQQWTADIARKRGIPMARLGKPQEPAQA 237
+ IR N + G E+ + + Q + K GIP+ +L KP + A A
Sbjct: 177 EYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETF--KTGIPLKKLAKPSDIADA 234

Query: 238 LLFLASPLASFTTGAALDVSGG 259
+LFL S A T L V GG
Sbjct: 235 VLFLVSGQAGHITMHNLCVDGG 256


100KPHS_43700KPHS_43630N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_43700-1144.073225di-/tripeptide transporter
KPHS_43690-1154.513683hypothetical protein
KPHS_43680-1154.285143putative outer membrane efflux protein MdtP
KPHS_43670-1133.271565multidrug efflux system protein MdtO
KPHS_43660-2151.455168multidrug resistance protein MdtN
KPHS_436500140.817850hypothetical protein
KPHS_436400141.356246hypothetical protein
KPHS_436302162.456170acyltransferase domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43700TCRTETB310.010 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.0 bits (70), Expect = 0.010
Identities = 33/188 (17%), Positives = 73/188 (38%), Gaps = 12/188 (6%)

Query: 33 SFYGIRPLLILFMAATVYDGGMGLARENASAIVGIFAGSMYLAALPGGWLADNWLGQQRA 92
SF+ + ++L ++ + + + F + + G L+D LG +R
Sbjct: 23 SFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ-LGIKRL 81

Query: 93 VWYGSILIALGHLSIALSAWLGNDLFFIGLMFIVL---GSGLFKTCISVMVGTLYKKGDA 149
+ +G I+ G ++ ++G+ F + +M + G+ F + V+V K
Sbjct: 82 LLFGIIINCFG----SVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK--E 135

Query: 150 RRDGGFSLFYMGINIGSFIAPLISGWLIKSHGWHWGFGIGGIGMLVALIIFRVFAVPSMK 209
R F L + +G + P I G + +H HW + + + + + F + +
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGG--MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEV 193

Query: 210 RYDAEVGL 217
R +
Sbjct: 194 RIKGHFDI 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43670GPOSANCHOR300.033 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 30.0 bits (67), Expect = 0.033
Identities = 33/148 (22%), Positives = 50/148 (33%), Gaps = 30/148 (20%)

Query: 489 DSEAPQLKTRLAALYRRLADCLAAPKEAVPLAPLLVAFTDSEALIHRVRAEPLGTYAHPW 548
+ + ++LAAL + + + K L A ++EA + E L
Sbjct: 399 EKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEA---KALKEKL------- 448

Query: 549 PQAKDWPMRATLAQAEEIARLSEGYRLNAAPGDPTLAR-------CAEQLRRYAERIEQE 601
AK QAEE+A+L G ++ D A Q + +
Sbjct: 449 --AK---------QAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAP 497

Query: 602 ATAPGEQL--TAELTNPFGPALAAALAA 627
QL T E NPF A A + A
Sbjct: 498 MKETKRQLPSTGETANPFFTAAALTVMA 525


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43660RTXTOXIND672e-14 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 66.8 bits (163), Expect = 2e-14
Identities = 50/362 (13%), Positives = 106/362 (29%), Gaps = 81/362 (22%)

Query: 11 KKWPLLALVLAAILALILVIWQL-----QTSPETNDAYVYADTIDVVPEVSGRIVEMPIR 65
+ P L +I I + + + ++ P + + E+ ++
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 66 DNQRVRKGDLLFRIDPRP---------------------YQAMLDDA------------- 91
+ + VRKGD+L ++ YQ +
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 92 ------------------KARLTTLDAQIMLTQRTIKAQEYNAQSVAAAVERARALVKQT 133
K + +T Q + + + +V A + R L +
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 134 TSTRIRLEPLVPQGFASQEDLDQARTAEKAARAELEATLLQAKQASAAVTGVDAMVAQRA 193
S L+ + ++ + + A EL Q +Q + +
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 194 GVL-------------------AQIALAELHLEFTEVRAPFNGVVVALKT-TVGQYASAL 233
+ ++A E + + +RAP + V LK T G +
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 234 KPVFTLL-DDDRWYVIANFRETDLNNVRPGVAARITVMT-NHNRT--FNGVVDSVGSGVL 289
+ + ++ +DD V A + D+ + G A I V + R G V ++ +
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAI 413

Query: 290 PE 291
+
Sbjct: 414 ED 415


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43630SACTRNSFRASE280.002 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.4 bits (63), Expect = 0.002
Identities = 11/55 (20%), Positives = 23/55 (41%)

Query: 11 YVNDAQGNQVAEIVFVPTGEHLSIIEHTDVDPSLKGQGVGKQLVAKVVEKMRQEQ 65
++ + N + I ++IE V + +GVG L+ K +E ++
Sbjct: 68 FLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENH 122


101KPHS_43310KPHS_43230N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_433100141.0238132-deoxy-D-gluconate 3-dehydrogenase
KPHS_433000130.185868low-affinity L-arabinose transport system proton
KPHS_43290-211-0.136770LacI transcriptional regulator
KPHS_43280-213-1.175054hypothetical protein
KPHS_43270-214-0.824530galactoside permease
KPHS_43260-113-1.283834glycoside hydrolase
KPHS_43250013-2.578775glycoporin RafY
KPHS_43240016-1.699864putative ABC transporter periplasmic binding
KPHS_43230-2150.058010ATPase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43310DHBDHDRGNASE1102e-31 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 110 bits (276), Expect = 2e-31
Identities = 72/257 (28%), Positives = 132/257 (51%), Gaps = 11/257 (4%)

Query: 3 LDAFSLQGKVAVVSGCDTGLGQGMALGLAEAGCDIVGI--NIVEPVETIERVTALGRRFL 60
++A ++GK+A ++G G+G+ +A LA G I + N + + + + A R
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 61 SLTADLRQIDGIPQLLERAVAEFGHIDILVNNAGLIRREDALAFSEKDWDDVMNLNIKSV 120
+ AD+R I ++ R E G IDILVN AG++R + S+++W+ ++N V
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 121 FFMSQAAAKHFIAQGSGGKIINIASMLSFQGGIRVPSYTASKSAVMGVTRLLANEWAKHN 180
F S++ +K+ + + G I+ + S + + +Y +SK+A + T+ L E A++N
Sbjct: 121 FNASRSVSKYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYN 179

Query: 181 INVNAIAPGYMATNNTQQLRADEQRSSEILD--------RIPAGRWGLPADLMGPVVFLA 232
I N ++PG T+ L ADE + +++ IP + P+D+ V+FL
Sbjct: 180 IRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

Query: 233 SSASDYINGYTVAVDGG 249
S + +I + + VDGG
Sbjct: 240 SGQAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43300TCRTETB553e-10 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 55.3 bits (133), Expect = 3e-10
Identities = 66/371 (17%), Positives = 131/371 (35%), Gaps = 34/371 (9%)

Query: 43 LDIGVISGALPFITDHFTLSSQLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAV 102
L+ V++ +LP I + F WV ++ ML +IG G LS +LG K L+ G +
Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87

Query: 103 LFVAGSIGSAFAAS-VEVLLVARVVLGVAVGIASYTAPLYLSEMASENVRGKMISMYQLM 161
+ GS+ S +L++AR + G + ++ + RGK + +
Sbjct: 88 INCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSI 147

Query: 162 VTLGIVLAFLSDTAFSYSGNWRAMLGVLALPAVILIILVVFLPNSPRWLAEKGRHIEAEE 221
V +G + ++ +W L L +I II V FL + H + +
Sbjct: 148 VAMGEGVGPAIGGMIAHYIHW----SYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKG 203

Query: 222 VLRMLRDTSEKARDELNEIRESLKLKQGGWALFKV----------------NRNVRRAVF 265
++ M + L + + +F N V
Sbjct: 204 IILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVL 263

Query: 266 LGMLLQAMQQFTGMNIIMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDK 325
G + G ++ Y + + +T E + ++ + +I VD+
Sbjct: 264 CGGI--IFGTVAGFVSMVPYMMK--DVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDR 319

Query: 326 AGRKPALKIGFSVMALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVV 385
G L IG + +++ L + T SW + + + G + + +
Sbjct: 320 RGPLYVLNIGVTFLSVSFLTASF----LLETT-----SWFMTIIIVFVLGGLSFTKTVIS 370

Query: 386 WILCSEIQPLK 396
I+ S ++ +
Sbjct: 371 TIVSSSLKQQE 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43270TCRTETA290.036 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 29.0 bits (65), Expect = 0.036
Identities = 31/142 (21%), Positives = 48/142 (33%), Gaps = 8/142 (5%)

Query: 43 AGDTGIIYAVLSVSALFAQVCYGFIQDKLGLRKHLLWYITALLILSGPAYLLFGHLLKIN 102
GI+ A+ ++ G + D+ G R LL L + Y + +
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLL----VSLAGAAVDYAIMATAPFLW 97

Query: 103 VL-LGSIFGGIYIGLTFNGGIGVLESYTERVARQSQFEFGRARMWGSLGWAVATFFAGLL 161
VL +G I GI G T + T+ R F F A G GL+
Sbjct: 98 VLYIGRIVAGI-TGATGAVAGAYIADITDGDERARHFGFMSACF--GFGMVAGPVLGGLM 154

Query: 162 FNINPQLNFLVASCSGLVFFIL 183
+P F A+ + F+
Sbjct: 155 GGFSPHAPFFAAAALNGLNFLT 176


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43240MALTOSEBP300.024 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 29.7 bits (66), Expect = 0.024
Identities = 72/298 (24%), Positives = 118/298 (39%), Gaps = 41/298 (13%)

Query: 128 NGKLNGIPISVTARVFYFNDEAWKKAGIPFPKTWDELMAAGKTFESKLGKQYYPVVLEHQ 187
NGKL PI+V A +N + PKTW+E+ A K ++K GK L+
Sbjct: 126 NGKLIAYPIAVEALSLIYNKDLLPNP----PKTWEEIPALDKELKAK-GKSALMFNLQEP 180

Query: 188 ----DVLALLNSYMVQKYNQPAIDEKGRKFSYSKAQWADFFGMYKKLIDSHVMPDTRYYA 243
++A Y KY D K + A+ F + + + H+ DT Y
Sbjct: 181 YFTWPLIAADGGYAF-KYENGKYDIKDVGVDNAGAKAGLTF-LVDLIKNKHMNADTDYSI 238

Query: 244 SFGKSNMYEMKPWIQGEWGGTYMWNSTINKYSDNLKPPAKLVLGEYPMLP--GATDAGLF 301
+ N E I G W + + S +N Y + P K P P G AG
Sbjct: 239 AEAAFNKGETAMTINGPWAWSNIDTSKVN-YGVTVLPTFK----GQPSKPFVGVLSAG-- 291

Query: 302 FKPAQMLSIGKSTKNPQAAAKVINFLLNSKEGVDILGLERGVPLSKAAVTYLTEDGVIKA 361
I ++ N + A + + L + EG++ + ++ PL A+ E+ A
Sbjct: 292 --------INAASPNKELAKEFLENYLLTDEGLEAVNKDK--PLGAVALKSYEEE---LA 338

Query: 362 DDPAVSGLKLAQSLPTALPVSPYFDDPQIVA---QFGTTLQYIDYGKKSVEEAAEDFQ 416
DP ++A ++ A + PQ+ A T + G+++V+EA +D Q
Sbjct: 339 KDP-----RIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQ 391


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_43230BACINVASINB363e-04 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 35.5 bits (81), Expect = 3e-04
Identities = 22/95 (23%), Positives = 44/95 (46%), Gaps = 10/95 (10%)

Query: 60 EVRIGDKIVNNLAPKSRGIAM-VFQNYALYPHMTVRENLAFGLKLSKLPKAQIDRQVEEA 118
+V +G ++ N A + G+A VF A E LA L++ QI + ++++
Sbjct: 504 KVALGMEVTNTAAQSAGGVAEGVFIKNA-------SEALA-DFMLARFAMDQIQQWLKQS 555

Query: 119 AKIL-ELEELLDRLPRQLSGGQAQRVAVGRAIVKK 152
+I E +++ L + +S Q R I+++
Sbjct: 556 VEIFGENQKVTAELQKAMSSAVQQNADASRFILRQ 590


102KPHS_35950KPHS_35800N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_35950-2132.147259putative dipeptide ABC transport system
KPHS_359400162.906421peptide ABC transporter ATP-binding protein
KPHS_359301223.901700lipid kinase
KPHS_359203274.670622putative protease
KPHS_359104275.492323two-component regulatory system response
KPHS_359004275.267218two-component regulatory system sensor protein
KPHS_358904254.323536multidrug efflux system protein MdtE
KPHS_358803243.789888multidrug efflux system subunit MdtC
KPHS_358702203.168221multidrug efflux system subunit MdtB
KPHS_358600141.999583multidrug efflux system subunit MdtA
KPHS_358501151.863718glutamate-aspartate symport protein
KPHS_358401143.169760putative positive transcription regulator
KPHS_358301143.372828hypothetical protein
KPHS_358201144.072260putative response regulator receiver
KPHS_358102154.022923putative signal transduction histidine kinase
KPHS_358002132.696675putative chaperone
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35950HTHFIS290.028 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 28.6 bits (64), Expect = 0.028
Identities = 11/25 (44%), Positives = 14/25 (56%)

Query: 47 IVGESGSGKSTVGRALLQLHPKKAR 71
I GESG+GK V RAL ++
Sbjct: 165 ITGESGTGKELVARALHDYGKRRNG 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35940PF05272300.008 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.008
Identities = 19/93 (20%), Positives = 28/93 (30%), Gaps = 35/93 (37%)

Query: 36 LVGESGSGKTTVLKCLAGLFTHWQGELTI---------------------------DAQP 68
L G G GK+T++ L GL I DA+
Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSEMTAFRRADAEA 660

Query: 69 LGHEISRERCRQVQMVFQDPYGSL---HPRHTI 98
+ S + R ++ YG HPR +
Sbjct: 661 VKAFFSSRKDR-----YRGAYGRYVQDHPRQVV 688


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35910HTHFIS764e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.4 bits (188), Expect = 4e-18
Identities = 31/148 (20%), Positives = 67/148 (45%), Gaps = 3/148 (2%)

Query: 11 PRILIVEDEPKLGQLLIDYLQAAGYAPALINHGDKVLPYVRQTPPHLILLDLMLPGTDGL 70
IL+ +D+ + +L L AGY + ++ + ++ L++ D+++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 71 TLCREIR-RFSDVPVVMVTAKIEEIDRLLGLEIGADDYICKPYSPREVVARVKTIL--RR 127
L I+ D+PV++++A+ + + E GA DY+ KP+ E++ + L +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 128 CKPQRDLQALDAQSPLIVDEGRFQASWR 155
+P + PL+ Q +R
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYR 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35900BCTERIALGSPF362e-04 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 36.3 bits (84), Expect = 2e-04
Identities = 28/93 (30%), Positives = 36/93 (38%), Gaps = 21/93 (22%)

Query: 198 LATLLAA-------------LATFPLARGLLAPVKRLVEGTHKLAA------GDFST--R 236
LATL+AA + P L+A V+ V H LA G F
Sbjct: 77 LATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLADAMKCFPGSFERLYC 136

Query: 237 VTVTGGDELGRLAQDFNQLASTLERNQQMRRDL 269
V G+ G L N+LA E+ QQMR +
Sbjct: 137 AMVAAGETSGHLDAVLNRLADYTEQRQQMRSRI 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35890TCRTETB1235e-33 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 123 bits (311), Expect = 5e-33
Identities = 93/435 (21%), Positives = 186/435 (42%), Gaps = 17/435 (3%)

Query: 20 FMQSLDTTIVNTALPSMAKSLGESPLHMHMIIVSYVLTVAVMLPASGWLADRVGVRNIFF 79
F L+ ++N +LP +A + P + + +++LT ++ G L+D++G++ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 80 TAIVLFTAGSLFCAQA-STLDQLVMARVLQGVGGAMMVPVGRLTVMKIVPRDQYMAAMTF 138
I++ GS+ S L+MAR +QG G A + + V + +P++ A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 139 VTLPGQVGPLLGPALGGVLVEYASWHWIFLINIP-VGIVGAIATLCLMPNYTMQTRRFDL 197
+ +G +GPA+GG++ Y HW +L+ IP + I+ + L+ FD+
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 198 SGFLLLAAGMATLTLALDGQKGLGISPAWLAGLVAVGLCALLLYLWHARGNARALFSLNL 257
G +L++ G+ L + ++ + V + + L+++ H R L
Sbjct: 202 KGIILMSVGIVFFMLF---------TTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGL 252

Query: 258 FRNRTFSLGLGGSFAGRIGSGMLPFMTPVFLQIGLGFSPFHAG-LMMIPMVLGSMGMKRI 316
+N F +G+ M P ++ S G +++ P + + I
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312

Query: 317 VVQVVNRFGYRRVLVASTLGLAAVSLLFMFSALAGWYYVLPLVLFLQGMINASRFSSMNT 376
+V+R G VL L+ L F +++ +++F+ G ++ ++ + ++T
Sbjct: 313 GGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTK-TVIST 371

Query: 377 LTLKDLPDDLASSGNSLLSMVMQLSMSIGVTIAGLLLGLYGQQHMSLDAASTHQVFLYT- 435
+ L A +G SLL+ LS G+ I G LL + L +LY+
Sbjct: 372 IVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSN 431

Query: 436 -YLSMAAIIALPALI 449
L + II + L+
Sbjct: 432 LLLLFSGIIVISWLV 446


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35880ACRIFLAVINRP8900.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 890 bits (2302), Expect = 0.0
Identities = 281/1035 (27%), Positives = 502/1035 (48%), Gaps = 36/1035 (3%)

Query: 6 LFIYRPVATILISLAITLCGILGFRLLPVAPLPQVDFPVIMVSASLPGASPETMASSVAT 65
FI RP+ ++++ + + G L LPVA P + P + VSA+ PGA +T+ +V
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 66 PLERSLGRIAGVNEMTSSS-SLGSTRIILEFNFDRDINGAARDVQAAINAAQSLLPSGMP 124
+E+++ I + M+S+S S GS I L F D + A VQ + A LLP +
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 125 SRPTYRKANPSDAPIMILTLTSDT--YSQGELYDFASTQLAQTIAQIDGVGDVDVGGSSL 182
+ S + +M+ SD +Q ++ D+ ++ + T+++++GVGDV + G+
Sbjct: 124 -QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY 182

Query: 183 PAVRVDLNPQALFNQGVSLDAVRTAISDANVRKPQG------ALEDSAHRWQVQTNDELK 236
A+R+ L+ L ++ V + N + G AL + K
Sbjct: 183 -AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 237 TAADYQPLIVHY-QNGAAVRLGDVATVSDSVQDVRNAGMTNAKPAILLMIRKLPEANIIQ 295
++ + + +G+ VRL DVA V ++ N KPA L I+ AN +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 296 TVDSIRARLPELQQTIPAAIDLQIAQDRSPTIRASLEEVEQTLVISVALVILVVFLFLRS 355
T +I+A+L ELQ P + + D +P ++ S+ EV +TL ++ LV LV++LFL++
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 356 GRATLIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMALTIATGFVVDDAIVVLENISRHL- 414
RATLIP +AVPV L+GTFA + G+S+N L++ + +A G +VDDAIVV+EN+ R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 EAGMKPLQAALQGSREVGFTVLSMSLSLVAVFLPLLLMGGLPGRLLREFAVTLSVAIGIS 474
E + P +A + ++ ++ +++ L AVF+P+ GG G + R+F++T+ A+ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 475 LAVSLTLTPMMCGWLLKSGKPHQPTRNRGFG----RLLVAVQGGYGKSLKWVLKHSRLTG 530
+ V+L LTP +C LLK GF Y S+ +L +
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 531 LVVLGTIALSVWLYISIPKTFFPEQDTGVLMGGIQADQSISFQ----AMRGKLQDFMKII 586
L+ +A V L++ +P +F PE+D GV + IQ + + + ++K
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 587 R-EDPAVDNVTGFT-GGSRVNSGMMFITLKPRDQRH---ETAQQVIDRLRKKLANEPGAN 641
+ +V V GF+ G N+GM F++LKP ++R+ +A+ VI R + +L
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 642 LFLMAVQDIRVGGRQSNASYQYTLLSDDLSALREWEPKIRKALA-----VLPELADVNSD 696
+ + I G + ++ L D + + R L L V +
Sbjct: 662 VIPFNMPAIVELGTATGFDFE---LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 697 QQDNGAEMDLVYDRDTMSRLGISVQDANNLLNNAFGQRQISTIYQPLNQYKVVMEVDPAY 756
++ A+ L D++ LG+S+ D N ++ A G ++ K+ ++ D +
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 757 TQDVSALDKMFVINSDGKPIPLAYFAKWQPANAPLSVNHQGLSAASTISFNLPTGRSLSE 816
+DK++V +++G+ +P + F + + I G S +
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 817 ASEAIDRAMTQLGVPSSVRGSFAGTAQVFQQTMNAQVILILAAIATVYIVLGVLYESYVH 876
A ++ ++L P+ + + G + + + N L+ + V++ L LYES+
Sbjct: 839 AMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSI 896

Query: 877 PLTILSTLPSAGVGALLALEIFDAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRNGN 936
P++++ +P VG LLA +F+ + ++G++ IG+ KNAI++V+FA +
Sbjct: 897 PVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 937 LTPEEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLGITIVGGLVMSQLL 996
EA A +R RPI+MT+LA + G LPL +S G GS + +GI ++GG+V + LL
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 997 TLYTTPVVYLFFDRL 1011
++ PV ++ R
Sbjct: 1017 AIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35870ACRIFLAVINRP8990.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 899 bits (2324), Expect = 0.0
Identities = 294/1036 (28%), Positives = 508/1036 (49%), Gaps = 29/1036 (2%)

Query: 13 SRLFIMRPVATTLLMVAILLAGIIGYRFLPVSALPEVDYPTIQVVTLYPGASPDVVTSAI 72
+ FI RP+ +L + +++AG + LPV+ P + P + V YPGA V +
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 73 TAPLERQFGQMSGLKQMSSQS-SGGASVVTLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131
T +E+ + L MSS S S G+ +TL FQ D+A+ +VQ + AT LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 132 LPNPPVYSKVNPADPPIMTLAVTSSAIPMTQVE--DMVETRVAQKISQVSGVGLVTLAGG 189
+ + S + +M S TQ + D V + V +S+++GVG V L G
Sbjct: 122 VQQQGI-SVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 190 QRPAVRVKLNAQAIAALGLTSETVRTAITSANVNSAKGSLDGP------ARAVTLSANDQ 243
Q A+R+ L+A + LT V + N A G L G ++ A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 244 MQSAEDYRRLII-AYQNGAPIRLGDVASVEQGAENSWLGAWANQQRAIVMNVQRQPGANI 302
++ E++ ++ + +G+ +RL DVA VE G EN + A N + A + ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 303 IDTADSIRQMLPQLTESLPKSVKVQVLSDRTTNIRASVRDTQFELMLAIALVVMIIYLFL 362
+DTA +I+ L +L P+ +KV D T ++ S+ + L AI LV +++YLFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 363 RNVPATIIPGVAVPLSLVGTFAVMVFLDFSINNLTLMALTIATGFVVDDAIVVIENISRY 422
+N+ AT+IP +AVP+ L+GTFA++ +SIN LT+ + +A G +VDDAIVV+EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 423 I-EKGEKPLAAALKGAGEIGFTIISLTFSLIAVLIPLLFMGDIVGRLFREFAVTLAVAIL 481
+ E P A K +I ++ + L AV IP+ F G G ++R+F++T+ A+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 482 ISAVVSLTLTPMMCARML---SHESLRKQNRFSRASERFFERVIAIYGRWLSRVLNHPWL 538
+S +V+L LTP +CA +L S E + F F+ + Y + ++L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 539 TLGVALSTLALSIILWVFIPKGFFPIQDNGIIQGTLQAPQSVSFASMAERQRQVASIILK 598
L + +A ++L++ +P F P +D G+ +Q P + + QV LK
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 599 DPA--VESLTSFVGVDGTNPALNSARLQINLKPLDERDDR---VQTVISRLQQAVDGVPG 653
+ VES+ + G + A N+ ++LKP +ER+ + VI R + + +
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 654 VALYLQPTQDLTIDTTVSRTQYQFTLQ---ANSLEALSTWVPPLLSRLQAQP-QLADVSS 709
++ P I + T + F L +AL+ LL P L V
Sbjct: 660 G--FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRP 717

Query: 710 DWQDKGLAAYIKVDRDSASRLGISMADVDNALYNAFGQRLISTIYTQANQYRVVLEQDTE 769
+ + ++VD++ A LG+S++D++ + A G ++ + ++ ++ D +
Sbjct: 718 NGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAK 777

Query: 770 ATPGLAALENIRLTSSDGGIVPLTAIATVEQRFTPLSVNHLDQFPVTTISFNVPDNYSLG 829
++ + + S++G +VP +A T + + + P I S G
Sbjct: 778 FRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG 837

Query: 830 EAVEAILAAEQSLDFPTDIRTQFQGSSLAFQSALGSTVWLVVAAVVAMYIVLGVLYESFI 889
+A+ + L P I + G S + + LV + V +++ L LYES+
Sbjct: 838 DAMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWS 895

Query: 890 HPITILSTLPTAGVGALLALWLAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQ 949
P++++ +P VG LLA L + DV ++G++ IG+ KNAI++++FA ++
Sbjct: 896 IPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKE 955

Query: 950 GMPPREAIYQACLLRFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLMLSQV 1009
G EA A +R RPILMT+LA +LG LPL +S G G+ + +GIG++GG++ + +
Sbjct: 956 GKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATL 1015

Query: 1010 LTLFTTPVIYLLFDRL 1025
L +F PV +++ R
Sbjct: 1016 LAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35860RTXTOXIND446e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.4 bits (105), Expect = 6e-07
Identities = 26/139 (18%), Positives = 52/139 (37%), Gaps = 16/139 (11%)

Query: 55 GAALAPVQAATATEEAVPRYLTGLGTVTAA-NTVTVRSRVDGQLLSLHFQEGQQVKAGDL 113
+A + + E V T G +T + + ++ + + + +EG+ V+ GD+
Sbjct: 67 FLVIAFILSVLGQVEIV---ATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDV 123

Query: 114 LAQIDPSQFKVALAQAQGQLAKDQATLANARRDLARYQQLVKTNLVSRQELDTQQSLVVE 173
L ++ A+ K Q++L AR + RYQ L ++ EL+ L +
Sbjct: 124 LLKLTAL-------GAEADTLKTQSSLLQARLEQTRYQILSRS-----IELNKLPELKLP 171

Query: 174 SAGTVKADEAAVASAQLQL 192
+ L
Sbjct: 172 DEPYFQNVSEEEVLRLTSL 190



Score = 36.3 bits (84), Expect = 2e-04
Identities = 26/170 (15%), Positives = 63/170 (37%), Gaps = 17/170 (10%)

Query: 125 ALAQAQGQLAKDQATLANARRDLARYQQLVKTNLVSRQELDTQQSLVVESAGTVKADEAA 184
+A +L ++ L ++ ++ + ++ +++ +
Sbjct: 260 KYVEAVNELRVYKSQLEQIESEILSAKEEY------QLVTQLFKNEILDKLRQTTDNIGL 313

Query: 185 V----ASAQLQLDWTRITAPIDGRV-GLKQVDIGNQISSGDTTGIVVLTQTHPIDVVFTL 239
+ A + + + I AP+ +V LK G +++ +T ++V ++V +
Sbjct: 314 LTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPED-DTLEVTALV 372

Query: 240 PESSIATVVQAQKAGKALSVEAWDRTNKQKISVGE--LLSLDNQIDATTG 287
I + Q A + VEA+ T + G+ ++LD D G
Sbjct: 373 QNKDIGFINVGQNA--IIKVEAFPYTRYGYLV-GKVKNINLDAIEDQRLG 419


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35820HTHFIS661e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 1e-14
Identities = 33/141 (23%), Positives = 62/141 (43%), Gaps = 6/141 (4%)

Query: 4 RLAIIEDNADLLDELLAWLGYRGFEVWGTRSAEAFWRQLHSHPVDIVLVDIGLPGEDGFS 63
+ + +D+A + L L G++V T +A WR + + D+V+ D+ +P E+ F
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 VLNYLHELGHY-GLVVVSARGQQQDKLQALSLGADAYLIKPVNFAH-LAETLTALGARLR 121
+L + + ++V+SA+ ++A GA YL KP + + AL R
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 122 QDRPAAPPAQ----AIGTPPA 138
+ +Q +G A
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAA 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_35800SHAPEPROTEIN461e-07 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 46.3 bits (110), Expect = 1e-07
Identities = 34/129 (26%), Positives = 57/129 (44%), Gaps = 20/129 (15%)

Query: 165 AMMLH-IKLQAESQLPEQIDQAVIGRPINFQGLGGDEANAQAQGILERAAHRAGFRDVVF 223
M+ H IK + + ++ P+ + E A + +A AG R+V
Sbjct: 89 KMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQV---ERRA-----IRESAQGAGAREVFL 140

Query: 224 QFEPVAAGLDFEATLSEEKRVLVVDIGGGTTDCSLLLMGPQWRERADRQQSLLGHSGCRI 283
EP+AA + +SE +VVDIGGGTT+ +++ + ++ S RI
Sbjct: 141 IEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN-----------GVVYSSSVRI 189

Query: 284 GGNDLDIAL 292
GG+ D A+
Sbjct: 190 GGDRFDEAI 198



Score = 35.1 bits (81), Expect = 4e-04
Identities = 30/121 (24%), Positives = 50/121 (41%), Gaps = 22/121 (18%)

Query: 365 RLSYRLV---RSAEESKIALSSA--ASVETALPFIQDDLATA------IAQQGLEAALDQ 413
R +Y + +AE K + SA + +LA + + AL +
Sbjct: 203 RRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQE 262

Query: 414 PLTRIMEQVRLALDSSQTTPDV--------IYLTGGSARSPLIKKALAAQLPGIPLAGGD 465
PLT I+ V +AL+ Q P++ + LTGG A + + L + GIP+ +
Sbjct: 263 PLTGIVSAVMVALE--QCPPELASDISERGMVLTGGGALLRNLDRLLMEET-GIPVVVAE 319

Query: 466 D 466
D
Sbjct: 320 D 320


103KPHS_32880KPHS_32800N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_32880-1173.527753putative acetyltransferase
KPHS_32870-3172.481645transcriptional regulator LysR
KPHS_32860-2172.354799major facilitator superfamily permease
KPHS_32850-3171.858844major facilitator superfamily permease
KPHS_32840-2171.885610molybdate ABC transporter periplasmic
KPHS_32830017-0.978068LysR family transcriptional regulator
KPHS_32820-1130.358031cold shock protein E
KPHS_328100142.508463putative ribosomal large subunit pseudouridine
KPHS_32800-1133.631353putative nucleoprotein/polynucleotide-associated
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32880SACTRNSFRASE443e-08 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 44.2 bits (104), Expect = 3e-08
Identities = 21/64 (32%), Positives = 26/64 (40%), Gaps = 2/64 (3%)

Query: 73 STWLGRNGIYMEDLYVTPDYRGIGAGKALLKTIAQYAVQRQCGRLEWSVLDWNQPAIDFY 132
S W G +ED+ V DYR G G ALL ++A + L D N A FY
Sbjct: 84 SNWNGY--ALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFY 141

Query: 133 LSIG 136

Sbjct: 142 AKHH 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32860TCRTETA320.001 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.5 bits (74), Expect = 0.001
Identities = 31/134 (23%), Positives = 53/134 (39%), Gaps = 18/134 (13%)

Query: 99 LMRPIGAIVLGAYIDKVGRRKGLIVTLSIMATGTFLIVLIPSYQTIGLWAPLLVLIGRLL 158
LM+ A VLGA D+ GRR L+V+L+ A ++ P LW ++ IGR++
Sbjct: 54 LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPF-----LW---VLYIGRIV 105

Query: 159 QGFSAGAELGGVSVYLAEIATSGRKGFYTSWQSGSQQVAIMVAAAMGFALNAVLEPSAIS 218
G + GA Y+A+I + + + S ++ +G +
Sbjct: 106 AGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGF------- 157

Query: 219 DWGWRIPFLFGCLI 232
PF +
Sbjct: 158 --SPHAPFFAAAAL 169


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32850TCRTETA351e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 35.2 bits (81), Expect = 1e-04
Identities = 39/157 (24%), Positives = 62/157 (39%), Gaps = 20/157 (12%)

Query: 48 ASDSLLVTLLVAISNFFWLPVGGALSDRFGRRSVLIAMTLLALATAWPALTMLANAPSFL 107
+ ++ L A+ F PV GALSDRFGRR VL L++LA A ++A AP
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVL----LVSLAGAAVDYAIMATAPFLW 97

Query: 108 MMLSVLLWLSFIYGMYNGAMIPALT----EIMPAEVRVAGFSLAYSLATAVFGGFT--PV 161
+ L++ I GA +I + R F ++ G PV
Sbjct: 98 V-----LYIGRIVAGITGATGAVAGAYIADITDGDERARHFGF---MSACFGFGMVAGPV 149

Query: 162 ISTALIEYTGDKASPGYWMSFAAICGLLATCYLYRRS 198
+ + ++ +P + + L C+L S
Sbjct: 150 LGGLMGGFS--PHAPFFAAAALNGLNFLTGCFLLPES 184


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32820PF05272290.011 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.9 bits (64), Expect = 0.011
Identities = 14/76 (18%), Positives = 32/76 (42%), Gaps = 15/76 (19%)

Query: 18 FIKDENGENRYFHVIKVANPDLIKKDAAVTFEPTTNNKGLSAYAVKVIPESKYIYIAGER 77
++ D G R++ V+ +L+ L + ++ E+ ++Y+AGER
Sbjct: 698 YLFDITGNRRFWPVLVPGRANLV---------------WLQKFRGQLFAEALHLYLAGER 742

Query: 78 LKLTSIKSYVVYREEE 93
+ + +R E+
Sbjct: 743 YFPSPEDEEIYFRPEQ 758


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32800TRNSINTIMINR290.013 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 28.9 bits (64), Expect = 0.013
Identities = 14/56 (25%), Positives = 32/56 (57%), Gaps = 2/56 (3%)

Query: 11 LKAGLVSSKKMAKVQRTAKKSRVQAREAREAVEENKKAQLERDKQLSEQQKQAVLA 66
+ +G + + ++ + AK++ AR+ +AVE N +AQ + Q + +Q++ L+
Sbjct: 308 IPSGELKDDIVEQIAQQAKEAGEVARQ--QAVESNAQAQQRYEDQHARRQEELQLS 361


104KPHS_32140KPHS_32110N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_32140-1140.735574hypothetical protein
KPHS_32130-1180.240134transcriptional regulator NarL
KPHS_321200170.409184two-component nitrate/nitrite sensory histidine
KPHS_321100200.116191nitrite extrusion protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32140INTIMIN2281e-68 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 228 bits (583), Expect = 1e-68
Identities = 116/408 (28%), Positives = 198/408 (48%), Gaps = 15/408 (3%)

Query: 31 PDMGIAPQVDDDARHFAEVAKKFGEASMSDNGLTAGEQAQLFAISKIGNEVSHQLESWLS 90
PD+ + DD A ++A + + L G+ A+ A+ GN+ S QL++WL
Sbjct: 150 PDVTKSNMTDDKALNYAAQQAASLGSQLQSRSLN-GDYAKDTALGIAGNQASSQLQAWLQ 208

Query: 91 PWGNANVDLLVDKEGKFTGSKGSWFVPLQDNDRYLTWNQYSVTRREHDLVGNIGLGQRWR 150
+G A V+L F GS + +P D+++ L + Q + N+G GQR+
Sbjct: 209 HYGTAEVNLQSGN--NFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFF 266

Query: 151 VGGWLLGYNSFYDKVLSESLARGSVGAEAWGEYLRLSANYYHPLGDW-QLRDNQTQEQRM 209
+ +LGYN F D+ S R +G E W +Y + S N Y + W + + + ++R
Sbjct: 267 LPENMLGYNVFIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERP 326

Query: 210 AAGYDVTAQARLPFYQHINTSVSVEQYFGDSVDLFHSGTGYHNPVAVSVGLNYTPVPLVT 269
A G+D+ LP Y + + EQY+GD+V LF+S NP A +VG+NYTP+PLVT
Sbjct: 327 ANGFDIRFNGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVT 386

Query: 270 VTAKHKQGENGVSQNNVGLKLNYRFGVPLKQQLAADEVAISNSLRGSRFDSPERDNLPVV 329
+ ++ G + ++ Y+F P QQ+ V +L GSR+D +R+N ++
Sbjct: 387 MGIDYRHGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIIL 446

Query: 330 EYRQRKNLTVYLATP-PWDLQSGETVQLKLQIHSLHGIKALHWQGDTQALSLTPPVDASS 388
EY +K + L P + T +++L + S +G+ + W D+ S + S
Sbjct: 447 EY--KKQDILSLNIPHDINGTERSTQKIQLIVKSKYGLDRIVWD-DSALRSQGGQIQHSG 503

Query: 389 PDG---WSIIMPVWNSEPGAANRWRLSVVVEDKQGQRVSSNEIALALT 433
+ I+P + G +N ++++ D+ G SSN + L +T
Sbjct: 504 SQSAQDYQAILPAYV--QGGSNVYKVTARAYDRNGN--SSNNVLLTIT 547


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32130HTHFIS748e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 74.5 bits (183), Expect = 8e-18
Identities = 35/118 (29%), Positives = 57/118 (48%), Gaps = 2/118 (1%)

Query: 6 RATILLIDDHPMLRTGVKQLISMAPDIQVIGEASNGAQGIELAESLDPDLILLDLNMPGM 65
ATIL+ DD +RT + Q +S A V SN A + D DL++ D+ MP
Sbjct: 3 GATILVADDDAAIRTVLNQALSRA-GYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 66 NGLETLDKLREKSLSGRVVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALQQA 123
N + L ++++ V+V S N + A ++GA YL K + +L+ + +A
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32120PF06580485e-08 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 48.3 bits (115), Expect = 5e-08
Identities = 28/116 (24%), Positives = 49/116 (42%), Gaps = 9/116 (7%)

Query: 476 FGFTVQLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASAT-----EVTVTVSQRDNQV 530
F +Q + Q+ P + L+Q E N +KH A ++ + ++ + V
Sbjct: 236 FEDRLQFENQINPAIMDVQVPPMLVQTLVE---NGIKHGIAQLPQGGKILLKGTKDNGTV 292

Query: 531 RLVVADNGRGVPDHAERSNHYGLIIMRDRAQSLRG-DCQVRRRETGGTEVIVTFIP 585
L V + G + + S GL +R+R Q L G + Q++ E G + IP
Sbjct: 293 TLEVENTGSLALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_32110TCRTETB310.011 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.0 bits (70), Expect = 0.011
Identities = 52/282 (18%), Positives = 93/282 (32%), Gaps = 49/282 (17%)

Query: 128 TPFSIFVIISLLCGFAGANF-ASSMANISFFFPKAKQGGALGVNGGLGNMGVSVMQLVAP 186
+ FS+ ++ + G A F A M ++ + PK +G A G+ G + MG V +
Sbjct: 101 SFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG 160

Query: 187 ------------LVVSISIFAVFGGNGSEQPDGS--------------------MLYLEN 214
L+ I+I V + + ML+ +
Sbjct: 161 MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTS 220

Query: 215 AAWIWVPFLIIFTLAAWFFMNDLSASK-----ASLSEQLPVLKRLHLWIMALLYLATFGS 269
+ FLI+ L+ F+ + L + +P + + + +A F S
Sbjct: 221 YSIS---FLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS 277

Query: 270 FIGFSAGFAM-LSKTQFPDVQILHYAFFGPFIGALARSMGGAISDRLGGTRVTLVNFVVM 328
+ + LS + V I G + GG + DR G V + +
Sbjct: 278 MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI----GGILVDRRGPLYVLNIGVTFL 333

Query: 329 AVFCALLFLTLPTNGQGGNFIAFFAVFMVLFLTAGLGSASTF 370
+V L T F+ VF++ L+ ST
Sbjct: 334 SVSFLTASFLLETTSW---FMTIIIVFVLGGLSFTKTVISTI 372


105KPHS_31430KPHS_31320N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_314300163.641023permease protein
KPHS_314200163.695863iron ABC transporter ATP-binding protein
KPHS_31410-1153.802936iron(III) dicitrate transport ATP-binding
KPHS_314000165.422597iron compound ABC superfamily ATP binding
KPHS_313900154.868830iron ABC transporter periplasmic iron-binding
KPHS_313801165.646134LysR family transcriptional regulator
KPHS_313700155.359390NAD dependent epimerase/dehydratase
KPHS_313601164.610979putative TetR family transcriptional regulator
KPHS_313501154.723020HlyD family secretion protein
KPHS_313402162.947522putative efflux protein
KPHS_31330219-0.084750L-lactate dehydrogenase
KPHS_31320341-13.631379hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31430TCRTETA667e-14 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 65.6 bits (160), Expect = 7e-14
Identities = 77/373 (20%), Positives = 136/373 (36%), Gaps = 30/373 (8%)

Query: 1 MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLI---GLILGLRTFSQQGMFILGGTLA 57
++L + + +G ++P L LRD ++ S + G++L L Q + G L+
Sbjct: 9 VILSTVALDAVGIGLIMPVLPGLLRD-LVHSNDVTAHYGILLALYALMQFACAPVLGALS 67

Query: 58 DRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGALFSPSIEALLARAGTH 117
DR+G + ++L + ++A LW + +G + G+ GA +
Sbjct: 68 DRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGA-------VAGAYI 120

Query: 118 SQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIALAGAGIFLLALAVLFFC 177
+ RA F + C G V GPV GG++ G A A + L F
Sbjct: 121 ADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFL 180

Query: 178 LPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSY------NQLYLALPV--EIQR 229
LP R PL R+ + ++ + + Q+ AL V R
Sbjct: 181 LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDR 240

Query: 230 SGGREQDLAPLFMLASLLIITLQLPLA-RFARRMGAVRILPVGFLLLSASFASVALFAAA 288
+ +L Q + A R+G R L +G + + +A
Sbjct: 241 FHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFA--- 297

Query: 289 PPAEGWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAHYGALATAGGCAVLA 348
GW+ P + LL G + + PA + ++ +E G G+LA +
Sbjct: 298 --TRGWM-AFPI---MVLLASGGIGM-PALQAMLSRQVDEERQGQLQGSLAALTSLTSIV 350

Query: 349 GNLLLGHLLDLAL 361
G LL + ++
Sbjct: 351 GPLLFTAIYAASI 363


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31410PF05272280.015 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 27.7 bits (61), Expect = 0.015
Identities = 10/20 (50%), Positives = 12/20 (60%)

Query: 33 LLGPNGCGKSSLLRVLAGLR 52
L G G GKS+L+ L GL
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31370NUCEPIMERASE376e-05 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 37.1 bits (86), Expect = 6e-05
Identities = 36/162 (22%), Positives = 60/162 (37%), Gaps = 28/162 (17%)

Query: 6 KVLILGASGGIGGEVARRLVADNWQVRA-----------LKRGAQMRDPEDGIQWIAGDA 54
K L+ GA+G IG V++RL+ QV LK+ + G Q+ D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 LDGGQVAA--AAAGCDVIVH-----AV-----NPPGYRHWRQQVLPMLRNTLQAAERQR- 101
D + A+ + + AV NP Y + L N L+ +
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYAD--SNLTGFL-NILEGCRHNKI 118

Query: 102 ALVVLPGTVYNYGPDA-FPLIAEEAAQQPVTRKGAIRVAMEL 142
++ + YG + P +++ PV+ A + A EL
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31360HTHTETR593e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.9 bits (142), Expect = 3e-13
Identities = 31/183 (16%), Positives = 52/183 (28%), Gaps = 10/183 (5%)

Query: 4 FSRYGYEKTTVTDLAKAIGFSKAYIYKFFDSKQAIGEAICASRLEKIMVAVSEAIADAPS 63
FS+ G T++ ++AKA G ++ IY F K + I I E A P
Sbjct: 24 FSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPG 83

Query: 64 ASEK-----LRRLFR-ALTEAGSELFFE--DRKLYDIAAVAARDKWPSTEQYAGHLQQLI 115
L + +TE L E K + +A + I
Sbjct: 84 DPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA--QRNLCLESYDRI 141

Query: 116 GQILVEGRQAGEFERKTPLDEATLAVYMVMCPFINPVQLQYNLDTAPTAAVLLASLILRS 175
Q L +A A + + + + A +++L
Sbjct: 142 EQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEM 201

Query: 176 LSP 178

Sbjct: 202 YLL 204


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31350RTXTOXIND423e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 3e-06
Identities = 19/92 (20%), Positives = 36/92 (39%), Gaps = 9/92 (9%)

Query: 70 GKVLERRVETGQSVKRGQLLLRLDPADLALQAQSQQRAVDAARARAKKAANDLARYRGLV 129
V E V+ G+SV++G +LL+L Q ++ AR L + R +
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQAR---------LEQTRYQI 155

Query: 130 ASGAISAAEFDQINAAAEAARADLSAAQAQAN 161
S +I + ++ E ++S +
Sbjct: 156 LSRSIELNKLPELKLPDEPYFQNVSEEEVLRL 187



Score = 31.3 bits (71), Expect = 0.005
Identities = 18/84 (21%), Positives = 32/84 (38%), Gaps = 4/84 (4%)

Query: 178 GVVVETLAEPGQVVSAGQVVIRLARAGQREARVQLPETLRPAVGSEALATRYGSESQPV- 236
+V E + + G+ V G V+++L G ++ +L A TRY S+ +
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQA---RLEQTRYQILSRSIE 161

Query: 237 TATLRLLSDAADATTRTFEARYVL 260
L L + + VL
Sbjct: 162 LNKLPELKLPDEPYFQNVSEEEVL 185



Score = 30.2 bits (68), Expect = 0.015
Identities = 12/128 (9%), Positives = 37/128 (28%), Gaps = 15/128 (11%)

Query: 103 SQQRAVDAARARAKKAANDLARYRGLVAS--GAISAAEFDQINAAAEA----------AR 150
++ ++ + A N+L Y+ + I +A+ +
Sbjct: 250 AKHAVLEQENKYVE-AVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTT 308

Query: 151 ADLSAAQAQANVAQNATGYAGLLADADGVVVE-TLAEPGQVVSAGQVVIRLARAGQR-EA 208
++ + + + + A V + + G VV+ + ++ + E
Sbjct: 309 DNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEV 368

Query: 209 RVQLPETL 216
+
Sbjct: 369 TALVQNKD 376


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31340ACRIFLAVINRP438e-139 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 438 bits (1128), Expect = e-139
Identities = 233/1055 (22%), Positives = 422/1055 (40%), Gaps = 71/1055 (6%)

Query: 8 LSALAVRERSVTLFLIILISVAGLVAFFGLGRAEDPPFTVKQMTVITVWPGATAQEMQDQ 67
++ +R L I++ +AG +A L A+ P ++V +PGA AQ +QD
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 68 VAEPLEKRLQELKWYDRTETYT-RPGMALITLSLQDQTPP----SEVPEQFYQARKKLGD 122
V + +E+ + + + + G ITL+ Q T P +V + A L
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLL-- 118

Query: 123 EAKNLPAGVSGPMMNDEFADVTFALFAL--KARGEPPRQLVRD--AEALRQQLLHVPGVK 178
P V ++ E + ++ + A + + D A ++ L + GV
Sbjct: 119 -----PQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVG 173

Query: 179 KVNILGEQ-AERIYLSFSHDRLATLGLSPEAIFAALNSQNVLTAAGAI---ETRGGQIF- 233
V + G Q A RI+L D L L+P + L QN AAG + GQ
Sbjct: 174 DVQLFGAQYAMRIWLDA--DLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLN 231

Query: 234 --IRLDGAFDRLQQIRDTPIIAG--GRTLKLADVATVERGYEDPATFLIRNQGEPALLLG 289
I F ++ + G ++L DVA VE G E+ R G+PA LG
Sbjct: 232 ASIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIA-RINGKPAAGLG 290

Query: 290 VVMREGWNGLALGKALDAETTSINQSLPLGMSLTKVTDQSVNISAAVDEFMIKFFVALLV 349
+ + G N L KA+ A+ + P GM + D + + ++ E + F A+++
Sbjct: 291 IKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIML 350

Query: 350 VMSVCFVSMG-WRVGVVVAAAVPLTLAVVFVVMEATGKNFDRITLGSLILALGLLVDDAI 408
V V ++ + R ++ AVP+ L F ++ A G + + +T+ ++LA+GLLVDDAI
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAI 410

Query: 409 IAIEMMV-VKMEEGYDRLKASAYAWSHTAAPMLAGTLVTAVGFMPNGFAQSTAGEYASNV 467
+ +E + V ME+ +A+ + S ++ +V + F+P F + G
Sbjct: 411 VVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQF 470

Query: 468 FWIVGIALIASWIVAVIFTPWLGVHLLPNRKPAAAGHAALYDT----------PRYQHFR 517
+ A+ S +VA+I TP L LL KP +A H H+
Sbjct: 471 SITIVSAMALSVLVALILTPALCATLL---KPVSAEHHENKGGFFGWFNTTFDHSVNHYT 527

Query: 518 RLLTRVIAHKWRVAAGVVALFIVAILGMSVVKKQFFPTSDRPEVLVEVQLPYGSSISQTS 577
+ +++ R + ++ + F P D+ L +QLP G++ +T
Sbjct: 528 NSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQ 587

Query: 578 AAAAKIEHWLHLQPEVKIVTSYIGQGAPRFYLAMAPELPDP--SFAKLMVLTDGQGARE- 634
++ + L+ E V S + + + + +F L + G
Sbjct: 588 KVLDQVTDYY-LKNEKANVESVFTVNG----FSFSGQAQNAGMAFVSLKPWEERNGDENS 642

Query: 635 --ALKRRLREAV-----ANGLAPEARVRVTQLVFGPYSPYPVAWRVMGPDPHALLDIAER 687
A+ R + + + V + + +G D AL +
Sbjct: 643 AEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHD--ALTQARNQ 700

Query: 688 VKSVLQASPL-MRTVNTDWGSRVPVMHFSLNQDRLQASGLSSQSVAQQLQFLLSGIPITT 746
+ + P + +V + ++Q++ QA G+S + Q + L G +
Sbjct: 701 LLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVND 760

Query: 747 VREDIRAVQVIGRAAGDIRLDPAKIADFTLVGSGGQRVPLSQIGDVSIRMEDPLLRRRDR 806
+ R ++ +A R+ P + + + G+ VP S P L R +
Sbjct: 761 FIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNG 820

Query: 807 TPTITVRGDVAENLQPPDVSTALMKPLQPIIDSLPPGYRIETAGSIEESGKATRAMVPLF 866
P++ ++G+ A P S M ++ + LP G + G + + L
Sbjct: 821 LPSMEIQGEAA----PGTSSGDAMALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALV 876

Query: 867 PIMIALTLLIIILQVRSLSAMVMVFLTAPLGLIGVVPTLLLFNQPFGINALVGLIALSGI 926
I + L + S S V V L PLG++GV+ LFNQ + +VGL+ G+
Sbjct: 877 AISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGL 936

Query: 927 LMRNTLILIGQIHHNQQA-GLDPFHAVVEATVQRARPVLLTALAAILAFIPLTHSVFWGT 985
+N ++++ + G A + A R RP+L+T+LA IL +PL S G+
Sbjct: 937 SAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGS 996

Query: 986 -----LAYTLIGGTLGGTIMTLIFLPAMYAIWFRI 1015
+ ++GG + T++ + F+P + + R
Sbjct: 997 GAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031



Score = 78.7 bits (194), Expect = 8e-17
Identities = 87/510 (17%), Positives = 181/510 (35%), Gaps = 36/510 (7%)

Query: 529 RVAAGVVALFIVA--ILGMSVVKKQFFPTSDRPEVLVEVQLPYGSSISQTSAAAAKIEHW 586
+ A V+A+ ++ L + + +PT P V V P + + IE
Sbjct: 9 PIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQN 68

Query: 587 LHLQPEVKIVTSY-IGQGAPRFYLAMAPELPDPSFAKLMVLTDGQGAREALKRRLREAVA 645
++ + ++S G+ L DP A++ V Q + L + V
Sbjct: 69 MNGIDNLMYMSSTSDSAGSVTITLTFQSGT-DPDIAQVQV----QNKLQLATPLLPQEVQ 123

Query: 646 NGLAPEARVRVTQLVFGPYSPYPVAWRVMGPDPHALLDIAER-VKSVLQASPLMRTVNTD 704
V + + + + D + D VK L + V
Sbjct: 124 Q---QGISVEKSSSSYLMVAGFVSDNPGTTQD--DISDYVASNVKDTLSRLNGVGDVQL- 177

Query: 705 WGSRVPVMHFSLNQDRLQASGLSSQSVAQQLQF----LLSGIPITTVREDIRAVQVIGRA 760
+G++ M L+ D L L+ V QL+ + +G T + + A
Sbjct: 178 FGAQY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 761 AGDIRLDPAKIADFTLVGSG-GQRVPLSQIGDVSIRMED-PLLRRRDRTPTITVRGDVAE 818
+ +P + TL + G V L + V + E+ ++ R + P + +A
Sbjct: 237 QTRFK-NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLAT 295

Query: 819 NLQPPDVSTALMKPLQPIIDSLPPGYRIE----TAGSIEESGKATRAMVPLFPIMIALTL 874
D + A+ L + P G ++ T ++ S +V I L
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLS---IHEVVKTLFEAIMLVF 352

Query: 875 LIIILQVRSLSAMVMVFLTAPLGLIGVVPTLLLFNQPFGINALVGLIALSGILMRNTLIL 934
L++ L ++++ A ++ + P+ L+G L F + G++ G+L+ + +++
Sbjct: 353 LVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVV 412

Query: 935 IGQIH-HNQQAGLDPFHAVVEATVQRARPVLLTALAAILAFIPL-----THSVFWGTLAY 988
+ + + L P A ++ Q ++ A+ FIP+ + + +
Sbjct: 413 VENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSI 472

Query: 989 TLIGGTLGGTIMTLIFLPAMYAIWFRIRPE 1018
T++ ++ LI PA+ A +
Sbjct: 473 TIVSAMALSVLVALILTPALCATLLKPVSA 502


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_31320HTHTETR647e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 64.3 bits (156), Expect = 7e-15
Identities = 34/172 (19%), Positives = 62/172 (36%), Gaps = 6/172 (3%)

Query: 7 QKGRPKDPLKTQAILQAARKLFLEQGLE-VTTAEIARVAGVAKATLYANFSDKEHLIEAV 65
+K + + Q IL A +LF +QG+ + EIA+ AGV + +Y +F DK L +
Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62

Query: 66 LRQESD--LTISDHDFAQRHHLPLIEVLTAFGYRFVRFINQRELTGWDRLIASAAVRHPD 123
+ A+ PL + + + + +I +
Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122

Query: 124 LPG--RFYAAGPGRAQQMLEAIIAEAIEAGTLRA-CDPQEAADELAGLWLGM 172
+ + + +E + IEA L A + AA + G G+
Sbjct: 123 MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGL 174


106KPHS_30430KPHS_30360N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_30430-118-3.142091acridine efflux pump
KPHS_30420-2171.935909putative acetyl transferase
KPHS_30410-1224.301412short chain dehydrogenase
KPHS_304001225.417869acetolactate synthase large subunit
KPHS_303901245.934344acetolactate decarboxylase
KPHS_303802257.114604LysR family transcriptional regulator
KPHS_303701257.5027493-hydroxybutyrate dehydrogenase
KPHS_30360-1247.330409citrate transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30430ACRIFLAVINRP10600.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1060 bits (2744), Expect = 0.0
Identities = 502/1031 (48%), Positives = 690/1031 (66%), Gaps = 7/1031 (0%)

Query: 1 MPHFFIERPIFAWVIALFIVLTGLLSIPRLPVAQYPEVAPPGIIISVSYPGASPEVMNTS 60
M +FFI RPIFAWV+A+ +++ G L+I +LPVAQYP +APP + +S +YPGA + + +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VVSLIEREISSVDNLLYFESSSDTTGMASITVTFKPGTDIKLAQMDLQNQIKIVESRLPQ 120
V +IE+ ++ +DNL+Y S+SD+ G +IT+TF+ GTD +AQ+ +QN++++ LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 SVRQNGINVEAANSGFLMMVGLKSPSGAYQEADLSDYFARNVTDELRRVPGVGKVQLFGG 180
V+Q GI+VE ++S +LM+ G S + + D+SDY A NV D L R+ GVG VQLFG
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 EKALRIWLDPMKLHSYGLSVTDVLSAISQQNVIVSPGRTGDEPATSSQEVTYPITVKGQL 240
+ A+RIWLD L+ Y L+ DV++ + QN ++ G+ G PA Q++ I + +
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 SSVEEFRNITIKSQVSAARVTLADVARVESGLQSYAFGIRENGVPATAAAIQLSPGANAI 300
+ EEF +T++ + V L DVARVE G ++Y R NG PA I+L+ GANA+
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 STASGIRARLTELSGVLPEGMTFTVPFDTAPFVKLSILKVVETFVEAMVLVFFVMLLFLH 360
TA I+A+L EL P+GM P+DT PFV+LSI +VV+T EA++LVF VM LFL
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 KIRCTLIPAIVAPVALLGTFTVMLLSGYSINILTMFGMILAIGIIVDDAIVVVENVERLM 420
+R TLIP I PV LLGTF ++ GYSIN LTMFGM+LAIG++VDDAIVVVENVER+M
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 EDKKMSPQDATREAMREITPAIIGITLVLTAVFIPMAFASGSVGIIYRQFSISMAISILL 480
+ K+ P++AT ++M +I A++GI +VL+AVFIPMAF GS G IYRQFSI++ ++ L
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SAFLALTLTPALCATLLKP-HGIHQGKSSVFSAWFNAHFHRLTSFYATGLGFVLKRTGRM 539
S +AL LTPALCATLLKP H F WFN F + Y +G +L TGR
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 540 MMIYAALCLALFAGLSTLPSSFLPDEDQGYFMSSIQLPSDATMQRTLKVVDTFEEEI--A 597
++IYA + + LPSSFLP+EDQG F++ IQLP+ AT +RT KV+D +
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 598 HRQAVESNIMILGFGFSGSGQNSAMAFTTLKDWRQRKGT--TAQEEADHIRSQMANVPDA 655
+ VES + GF FSG QN+ MAF +LK W +R G +A+ + ++ + D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 656 VTMSLLPPAISDMGTSSGFTYYLQDRGGKGYQALKKAADELIVQANHNP-HLADVYIDGL 714
+ PAI ++GT++GF + L D+ G G+ AL +A ++L+ A +P L V +GL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 715 GEGTSLSLHVDREKAEAMGVSFDEINQTISVAAGSNYVNDYTNNGRVQQVIVQADAPYRM 774
+ L VD+EKA+A+GVS +INQTIS A G YVND+ + GRV+++ VQADA +RM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 775 QPEQLLALSVKNRLGQMLPLSTFVTLSWNVAPQQLIRYQGYPAIRITGSAAQGKSSGTAM 834
PE + L V++ G+M+P S F T W +L RY G P++ I G AA G SSG AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 835 AAMDNLAKHLPPGFAGEWAGSSLQEKESASQLPGLIVLSVLVVFMVLAALYESWSVPFAV 894
A M+NLA LP G +W G S QE+ S +Q P L+ +S +VVF+ LAALYESWS+P +V
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 895 MLVVPLGLLGAVLAVSVTNMTNDVFFKVGLITLIGLSAKNAILIIEFARQLM-KEGKSLI 953
MLVVPLG++G +LA ++ N NDV+F VGL+T IGLSAKNAILI+EFA+ LM KEGK ++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 954 DATLTAAKLRLRPILMTSLAFTLGVVPLMLASGASDSTQHAIGTGVFGGMISGTLLAIFF 1013
+ATL A ++RLRPILMTSLAF LGV+PL +++GA Q+A+G GV GGM+S TLLAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1014 VPVFFVTITRF 1024
VPVFFV I R
Sbjct: 1021 VPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30410DHBDHDRGNASE1241e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (312), Expect = 1e-36
Identities = 74/254 (29%), Positives = 114/254 (44%), Gaps = 10/254 (3%)

Query: 3 KVALVTGAGQGIGKAIALRLVKDGFAVAIADYNDATAKAVASEINQAGGRAMAVKVDVSD 62
K+A +TGA QGIG+A+A L G +A DYN + V S + A A DV D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 63 RDQVFAAVEQARKTLGGFDVIVNNAGVAPSTPIESITPEIVDKVYNINVKGVIWGIQAAV 122
+ + + +G D++VN AGV I S++ E + +++N GV ++
Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128

Query: 123 EAFKKEGHGGKIINACSQAGHVGNPELAVYSSSKFAVRGLTQTAARDLAPLGITVNGYCP 182
+ G I+ S V +A Y+SSK A T+ +LA I N P
Sbjct: 129 KYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 183 GIVKTPM----WAEIDRQVSEAAGKPLGYGTAEFAKRITLGRLSEPEDVAACVSYLASPD 238
G +T M WA+ + G F I L +L++P D+A V +L S
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGS-----LETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 239 SDYMTGQSLLIDGG 252
+ ++T +L +DGG
Sbjct: 243 AGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30370DHBDHDRGNASE1154e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 115 bits (288), Expect = 4e-33
Identities = 66/255 (25%), Positives = 105/255 (41%), Gaps = 9/255 (3%)

Query: 3 LHGKTALVTGSTSGIGLGIAKVLAQAGAQLVLNGFGDSSHARAE--VAALGKIPGYHDAD 60
+ GK A +TG+ GIG +A+ LA GA + + + + A + AD
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 61 LRDVGQIEAMMRYAESTFGGVDIVINNAGIQHVAPVEQFPVDKWNDILAINLSSVFHTTR 120
+RD I+ + E G +DI++N AG+ + ++W ++N + VF+ +R
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 121 LALPGMRQRNWGRIINIASVHGLVASKEKSAYVAAKHAVVGLTKTVALETARSGITCNAI 180
M R G I+ + S V +AY ++K A V TK + LE A I CN +
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 181 CPGWVLTPLVQQQIDKRIAEGVDPEQASAQLLAEKQ---PSGEFVTPQQLGEMALFLCSD 237
PG T + EQ L + P + P + + LFL S
Sbjct: 186 SPGSTETDMQWSLWADENGA----EQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 238 AAAQVRGAAWNMDGG 252
A + +DGG
Sbjct: 242 QAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30360ABC2TRNSPORT300.028 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 29.5 bits (66), Expect = 0.028
Identities = 32/129 (24%), Positives = 47/129 (36%), Gaps = 15/129 (11%)

Query: 7 ALAALALLMLAAYRGY----SVILFAPIAALGAVLLTDPGAVGPA----------FTGLF 52
ALA + ++AA GY S++ P+ AL + G V A + L
Sbjct: 126 ALAGAGIGVVAAALGYTQWLSLLYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLV 185

Query: 53 MEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAIRILGRRHAIPVIVLVCALLTY 112
+ ++ FPV L VF S SI +LG + V V AL Y
Sbjct: 186 ITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIMLGHP-VVDVCQHVGALCIY 244

Query: 113 GGVSLFVVA 121
+ F+
Sbjct: 245 IVIPFFLST 253


107KPHS_30090KPHS_30040N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_300900141.826391major facilitator superfamily protein
KPHS_300801142.923805putative transport protein
KPHS_300702153.254582hypothetical protein
KPHS_300602153.300833hypothetical protein
KPHS_30050-1152.816934hypothetical protein
KPHS_300400163.139036oligopeptide ABC transporter ATP-binding
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30090TCRTETB673e-15 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 66.8 bits (163), Expect = 3e-15
Identities = 41/163 (25%), Positives = 72/163 (44%), Gaps = 3/163 (1%)

Query: 39 PAIQQSLGGSPAALSWLTNGFMLTFGSFLLAAGVTADAIDRKRIFIAGAALLCLSSLLFC 98
P I PA+ +W+ FMLTF G +D + KR+ + G + C S++
Sbjct: 38 PDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGF 97

Query: 99 LTHNLFLSGVL-RALQGLAAAMILASGSAALAQLYDGAQRTRAFSILGTVFGIGLAFGPL 157
+ H+ F ++ R +QG AA A +A+ R +AF ++G++ +G GP
Sbjct: 98 VGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPA 157

Query: 158 LIGFMIDAVGWRGVYALFALLSAGVLLIGLVSLPATEKASRGH 200
+ G + + W + + + V L+ L E +GH
Sbjct: 158 IGGMIAHYIHWSYLLLIPMITIITVPF--LMKLLKKEVRIKGH 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30080TCRTETB452e-07 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 44.9 bits (106), Expect = 2e-07
Identities = 36/195 (18%), Positives = 76/195 (38%), Gaps = 10/195 (5%)

Query: 14 GFLSLTTLALLIASGGLFVAFVVRCRRVNNPVLELSLLRHPRFVGVLLLPVATCCCYVVL 73
FL ++ L+ LI FV R+V +P ++ L ++ F+ +L
Sbjct: 224 SFLIVSVLSFLI--------FVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGF 275

Query: 74 LIIVPLHFMGGEGMSESQ-SALYLMALTTPMLVFPSVAALLTRWFSPGQVSTAGLMMASV 132
+ +VP +S ++ ++ + T +++F + +L P V G+ SV
Sbjct: 276 VSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV 335

Query: 133 GLLLLGDAFHSNHLPQLVLALILCGAGAALPWGLMDGLAISAVPVAKAGMAAGLFNTVRV 192
L + + ++ G + ++ + S++ +AG L N
Sbjct: 336 SFLTASFLLETTSWFM-TIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSF 394

Query: 193 AGEGIALAVVSAVLT 207
EG +A+V +L+
Sbjct: 395 LSEGTGIAIVGGLLS 409



Score = 28.3 bits (63), Expect = 0.036
Identities = 20/89 (22%), Positives = 37/89 (41%), Gaps = 3/89 (3%)

Query: 121 QVSTAGLMMASVGLLLLGD---AFHSNHLPQLVLALILCGAGAALPWGLMDGLAISAVPV 177
Q+ L++ + + G + L++A + GAGAA L+ + +P
Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK 134

Query: 178 AKAGMAAGLFNTVRVAGEGIALAVVSAVL 206
G A GL ++ GEG+ A+ +
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIA 163


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30050cloacin310.002 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 30.8 bits (69), Expect = 0.002
Identities = 15/44 (34%), Positives = 20/44 (45%)

Query: 23 PAYANPGNGNGNGGGNHGNSGNHGNSGNHGNNGNSGDHGNKGQN 66
+++ N G G G+ + G GN G NGNSG G N
Sbjct: 37 SGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80



Score = 29.7 bits (66), Expect = 0.006
Identities = 13/39 (33%), Positives = 16/39 (41%)

Query: 27 NPGNGNGNGGGNHGNSGNHGNSGNHGNNGNSGDHGNKGQ 65
NP G G + G HGN G +GN+G G
Sbjct: 44 NPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82



Score = 29.7 bits (66), Expect = 0.007
Identities = 11/32 (34%), Positives = 16/32 (50%)

Query: 26 ANPGNGNGNGGGNHGNSGNHGNSGNHGNNGNS 57
+ G G+G GN G +GN G G N ++
Sbjct: 52 SGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_30040PF05272280.038 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.038
Identities = 16/49 (32%), Positives = 22/49 (44%), Gaps = 4/49 (8%)

Query: 33 VGGVSFNIRPGTIFG----LVGESGSGKTTVGRTLLGLYEKSAGSVKFH 77
+G V+ + PG F L G G GK+T+ TL+GL S
Sbjct: 582 MGHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIG 630


108KPHS_26350KPHS_26280N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_263502151.1781923-oxoacyl-(acyl-carrier-protein) reductase
KPHS_263401132.072093putative acetyl-CoA acetyltransferase
KPHS_263300140.282710major facilitator family transporter
KPHS_26320-1131.208590major facilitator family transporter
KPHS_26310-1141.981460putative gluconolactonase
KPHS_263001142.171956putative general substrate transporter
KPHS_262902162.255210glycerate kinase
KPHS_262800150.705225putative sugar diacid utilization regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26350DHBDHDRGNASE1241e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (311), Expect = 1e-36
Identities = 74/254 (29%), Positives = 121/254 (47%), Gaps = 12/254 (4%)

Query: 3 LASKTAIVTGAARGIGFGIAQVLAREGARVIIADRDAHG-EAAAASLRESGAQALFISCN 61
+ K A +TGAA+GIG +A+ LA +GA + D + E +SL+ A +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 62 IAEKTQVEALFSQAEEAFGPVDILVNNAGINRDAMLHKLTEADWDTVIDVNLKGTFLCMQ 121
+ + ++ + ++ E GP+DILVN AG+ R ++H L++ +W+ VN G F +
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 122 QAAIRMRERGAGRIINIAS-ASWLGNVGQTNYSASKAGVVGMTKTACRELAKKGVTVNAI 180
+ M +R +G I+ + S + + Y++SKA V TK ELA+ + N +
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 181 CPGFIDTDMTRG--VPENVWQIMIS--------KIPAGYAGEAKDVGECVAFLASDGARY 230
PG +TDM EN + +I IP + D+ + V FL S A +
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 231 INGEVINVGGGMVL 244
I + V GG L
Sbjct: 246 ITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26330VACCYTOTOXIN260.045 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 26.1 bits (57), Expect = 0.045
Identities = 10/24 (41%), Positives = 18/24 (75%)

Query: 13 QLQRTHKKIYRHLMPLLIVAYIIS 36
++Q+TH+KI R L+ L +V ++S
Sbjct: 2 EIQQTHRKINRPLVSLALVGALVS 25


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26320TCRTETB300.016 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.8 bits (67), Expect = 0.016
Identities = 26/157 (16%), Positives = 48/157 (30%), Gaps = 13/157 (8%)

Query: 131 STRVFLLLVLIYFTHQFSVYGLSYFLPGIIGSWGQLTPLQVGLLTAIP-----WIAAAAG 185
F++ VL +V G +P ++ QL+ ++G + P I G
Sbjct: 254 KNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIG 313

Query: 186 GILLPRFARTEQRSRSMLMAGYLVMATGMAIGAIAGHG---VALLGFSLAAFMFFAMQSI 242
GIL+ R +L G ++ + + +++
Sbjct: 314 GILVDRRG-----PLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTV 368

Query: 243 IFNWLPSIMSGHMLAGSFGLLNCLGLCGGFLGPFILG 279
I + S + LLN G I+G
Sbjct: 369 ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVG 405


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26300TCRTETB392e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 39.5 bits (92), Expect = 2e-05
Identities = 71/399 (17%), Positives = 134/399 (33%), Gaps = 51/399 (12%)

Query: 53 EMILRLG-PVISKEFSLSPEQWGNIVALIMVALAVLDIPGSIWSDRYGSGWKRARFQVPL 111
EM+L + P I+ +F+ P + M+ ++ SD+ G + L
Sbjct: 30 EMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLG-------IKRLL 82

Query: 112 VLGYTALSFISGIKAISHGLTAFVLL-RVGVNLGAGWGEPVGVSNTAEWWPKEKRGFALG 170
+ G F S I + H + +++ R GA + + A + PKE RG A G
Sbjct: 83 LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFG 142

Query: 171 VHHTGYPIGALLSGVVASLVLATFGEGSWRYCFLL--ALLVAIPLMIFWAKYSTADRINT 228
+ + +G + + ++ W Y L+ ++ +P ++ K RI
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIH---WSYLLLIPMITIITVPFLMKLLK--KEVRIK- 196

Query: 229 LYQHIDSQG----------LTRPATQES---------------SHVAKGEGMKTFLRTLR 263
H D +G T S H+ K +
Sbjct: 197 --GHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGK 254

Query: 264 NRNISLTAGNTLLTQIVYMGINVVLPPYLYHVSGLSLAASAGLSIIF--TLTGTLGQVIW 321
N + + G ++P + V LS A G IIF T++ + I
Sbjct: 255 NIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAE-IGSVIIFPGTMSVIIFGYIG 313

Query: 322 PWLSDSFGRKRTLIVCGLWMSIG---IALFYFATNMPRLIAIQLFFGLVANAVWPIYYAM 378
L D G L + ++S+ + T+ I I G ++ + +
Sbjct: 314 GILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLS-FTKTVISTI 372

Query: 379 ASDSAEERATSTANGIITTAMFIGGGISPLLMGWLIQFG 417
S S +++ ++ F+ G ++G L+
Sbjct: 373 VSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIP 411


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_26280DNABINDNGFIS290.012 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 28.8 bits (64), Expect = 0.012
Identities = 13/50 (26%), Positives = 30/50 (60%), Gaps = 2/50 (4%)

Query: 296 INNVRQLLEHDSGEVLLDTLSSFIANNAEPGKTSLLLGIHRNTLTYRLQQ 345
+N++ +L+ + + LLD + + N + +L++GI+R TL +L++
Sbjct: 47 VNDLYELVLAEVEQPLLDMVMQYTRGNQT--RAALMMGINRGTLRKKLKK 94


109KPHS_17120KPHS_17060N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_171202152.671487putative DEOR-type transcriptional regulator
KPHS_171100141.625201putative DEOR-type transcriptional regulator
KPHS_171000140.191576putative hydrolase
KPHS_170900140.816748major facilitator superfamily protein
KPHS_17080-1111.384894undecaprenyl pyrophosphate phosphatase
KPHS_17070-1130.174970DNA-binding transcriptional repressor DeoR
KPHS_17060013-0.997414D-alanyl-D-alanine carboxypeptidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17120HTHTETR504e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 50.4 bits (120), Expect = 4e-10
Identities = 17/76 (22%), Positives = 33/76 (43%), Gaps = 2/76 (2%)

Query: 1 MAR--RPNDPQRRERILQATLDTIAAHGIHAVTHRKIATCANVPLGSLTYYFSGIEALIE 58
MAR + + R+ IL L + G+ + + +IA A V G++ ++F L
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 59 EAFSLFTAEMSAQYQQ 74
E + L + + +
Sbjct: 61 EIWELSESNIGELELE 76


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17110TCRTETB348e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.1 bits (78), Expect = 8e-04
Identities = 34/155 (21%), Positives = 62/155 (40%), Gaps = 19/155 (12%)

Query: 17 LFMFFFIPGLLMASWATRTPAIRDLLALSTAEMGVVLFGLSVGSMSGILCS---AWLVKR 73
+ I G + + ++D+ LSTAE+G V+ + G+MS I+ LV R
Sbjct: 262 VLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVI--IFPGTMSVIIFGYIGGILVDR 319

Query: 74 FGTRKVIRTTM-----SFAVLGMLVLSLALWVTSAPLFAFGLAIFGASFGSAEVAINVEG 128
G V+ + SF L+ + + ++T +F G F + S V+ +++
Sbjct: 320 RGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQ 379

Query: 129 AAIEREMNKTVLPMMHGFYSFGTLFGAGVGMAVTG 163
M +F + G G+A+ G
Sbjct: 380 QEAGAGM---------SLLNFTSFLSEGTGIAIVG 405



Score = 30.2 bits (68), Expect = 0.016
Identities = 30/150 (20%), Positives = 63/150 (42%), Gaps = 6/150 (4%)

Query: 218 LLIGVIVLAMAFAEGSANDWL-PLLMVDGHGFSP-TSGSLIYAGFTLGMTLGRFTGGWFI 275
+IGV+ + F + + P +M D H S GS+I T+ + + + GG +
Sbjct: 258 FMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILV 317

Query: 276 DRYSRVAVVRGSAV---MGALGIGLIIFVDNPWVAGISVLLWGIGASLGFPLTISAASDT 332
DR + V+ + L ++ + ++ I V + G + ++ +S
Sbjct: 318 DRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSL 377

Query: 333 GP-DAPKRVSVVAITGYLAFLVGPPLLGFL 361
+A +S++ T +L+ G ++G L
Sbjct: 378 KQQEAGAGMSLLNFTSFLSEGTGIAIVGGL 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17090TCRTETA454e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 44.8 bits (106), Expect = 4e-07
Identities = 61/267 (22%), Positives = 106/267 (39%), Gaps = 19/267 (7%)

Query: 71 LLGPLSDRIGRRPVMLTGVVWFIVTCLATLLAQTIEQFTLLRFLQGISLCFIGAVGYAAI 130
+LG LSDR GRRPV+L + V A + + R + GI+ GAV A I
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGA-TGAVAGAYI 120

Query: 131 QESFEEAVCIKITALMANVALIAPLLGPLVGAAWVHVLPWEMMFVLFAVLAAISFFGLQR 190
+ + + M+ + GP++G P F A L ++F
Sbjct: 121 ADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSP-HAPFFAAAALNGLNFLTGCF 179

Query: 191 AMPET--ATRLGEKLSVKELGRDYRLVLKNLRFVAGALATGFVSLPLLAWIAQSP--VII 246
+PE+ R + +R + + VA +A F ++ + Q P + +
Sbjct: 180 LLPESHKGERRPLRREALNPLASFRWA-RGMTVVAALMAVFF----IMQLVGQVPAALWV 234

Query: 247 ISGEQATSYEYGMLQVPI--FGAL--IAGNLVLARLTARRTVRSLIIMGGWPIMFGLILS 302
I GE ++ + + + FG L +A ++ + AR R +++G G IL
Sbjct: 235 IFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILL 294

Query: 303 AAATVVSSHAYLWMTAGLSFYAFGIGL 329
A AT ++ + + GIG+
Sbjct: 295 AFAT----RGWMAFPIMVLLASGGIGM 317


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_17060BLACTAMASEA354e-04 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 34.8 bits (80), Expect = 4e-04
Identities = 44/192 (22%), Positives = 70/192 (36%), Gaps = 24/192 (12%)

Query: 15 CALLFLVAPAV-QAAEQLPDAPS-IDAR-AWILMDYASGKVLSEGNADEKLDPASLTKIM 71
A L L A Q EQ+ + S + R I MD ASG+ L+ ADE+ S K++
Sbjct: 12 LATLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTFKVV 71

Query: 72 TSYVVGQAIKAGKIKLTDMVTVGRDAWATGNPALRGSSVMFLKPGMQVSVEDLNKGVIIQ 131
V + AG +L + + +P L GM +V +L I
Sbjct: 72 LCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSE----KHLADGM--TVGELCAAAITM 125

Query: 132 SGNDASIAIADYVAGSQDAFVSLMNGYAKKMGLTNTTFMTVH-----GLDAPGQF---ST 183
S N A+ + V G + + +++G + PG +T
Sbjct: 126 SDNSAANLLLATVGGPAG-----LTAFLRQIG--DNVTRLDRWETELNEALPGDARDTTT 178

Query: 184 ARDMALLTKALI 195
MA + L+
Sbjct: 179 PASMAATLRKLL 190


110KPHS_16560KPHS_16470N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_16560517-4.049535putative short-chain dehydrogenase/reductase
KPHS_16550317-2.847359putative general substrate transporter
KPHS_165400150.703275hypothetical protein
KPHS_16530-1151.014961transcriptional regulator LysR
KPHS_16520-2161.260420putative DNA-binding transcriptional regulator
KPHS_16510-1151.321356putative DNA-binding transcriptional regulator
KPHS_16500-2151.468061hypothetical protein
KPHS_16490-3141.018404putative ABC-type transport system ATP-binding
KPHS_16480-1150.405745ABC transporter membrane protein
KPHS_16470-1140.004837putative ABC-type transport system inner
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16560DHBDHDRGNASE1182e-34 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 118 bits (296), Expect = 2e-34
Identities = 74/255 (29%), Positives = 119/255 (46%), Gaps = 16/255 (6%)

Query: 3 LKDKVAIITGAASARGLGFATAKLFAENGAKVVIIDLNGEAS---KTAAAALGEGHLGLA 59
++ K+A ITGAA +G+G A A+ A GA + +D N E ++ A
Sbjct: 6 IEGKIAFITGAA--QGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 60 ANVADEVQVQAAIEQILAKYGRVDVLVNNAGITQPLKLMDIKRANYDAVLDVSLRGTLLM 119
A+V D + +I + G +D+LVN AG+ +P + + ++A V+ G
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 120 SQAVIPTMRAQKSGSIVCISSVSAQRGGGIFGGPHYSAAKAGVLGLARAMARELGPDNVR 179
S++V M ++SGSIV + S A G Y+++KA + + + EL N+R
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181

Query: 180 VNCITPGLIQTDITAGKLTDD---------MTANILAGIPMNRLGDAIDIARAALFLGSD 230
N ++PG +TD+ D+ GIP+ +L DIA A LFL S
Sbjct: 182 CNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241

Query: 231 LSSYSTGITLDVNGG 245
+ + T L V+GG
Sbjct: 242 QAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16550TCRTETA441e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 43.7 bits (103), Expect = 1e-06
Identities = 57/400 (14%), Positives = 128/400 (32%), Gaps = 50/400 (12%)

Query: 22 LTMIFLVYAINYADRTNIGAVLPFIIDEFHINNFEAGAIASMFFLGYAVSQIP----AGF 77
L +I A++ I VLP ++ + +N + L YA+ Q G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLAL-YALMQFACAPVLGA 65

Query: 78 FIAKRGTRGLVSLSIFGFSAFTWLMGTVSSVFGLKLVRLGLGLSEGPCPVGLASTINNWF 137
+ G R ++ +S+ G + +M T ++ L + R+ G++ V + I +
Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAV-AGAYIADIT 124

Query: 138 PPKEKATATGVYIAATMFAPIIVPPLAVWIAVTWGWRWVFFSFAIPGIVAAIAWYLLVKS 197
E+A G +++A ++ P+ + + FF+ A + + L+
Sbjct: 125 DGDERARHFG-FMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL-- 181

Query: 198 KPAESGFVSQSELAEINAGRESHNNSVR-ENILIAERFTWLDKIIRVKKMAPIDTAKGLF 256
ESH R + +A + +
Sbjct: 182 -------------------PESHKGERRPLRREALNPLASFRWARGMTVVAAL-----MA 217

Query: 257 TSKNILGDCLAYFMMVSVLYGLLTWIPLYLVKERGFDVMSMGFVASMPCIGGFIGAIGGG 316
+F+M V ++ +D ++G + G + ++
Sbjct: 218 V----------FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLA---AFGILHSLAQA 264

Query: 317 WVSDKLLGR-RRKPTMMFTAVSTVVMMLIMLNIPASTLAVCIGLFFVGFCLNIGWPAFTA 375
++ + R + +M ++ +++ +A I + IG PA A
Sbjct: 265 MITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASG--GIGMPALQA 322

Query: 376 YGMAVSDSKTYPIASSIINSGGNLGGFVAPMAAGFLLDKT 415
D + + + +L V P+ + +
Sbjct: 323 MLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAAS 362


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16520HTHTETR585e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.5 bits (141), Expect = 5e-14
Identities = 16/74 (21%), Positives = 33/74 (44%), Gaps = 1/74 (1%)

Query: 4 KGEQAKNQLIAAAIAQFGEYGQHATT-RDIAAQAGQNIAAITYYFGSKDDLYLACAQWIA 62
+ ++ + ++ A+ F + G +T+ +IA AG AI ++F K DL+ +
Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67

Query: 63 DFIGDNFARRPKRR 76
IG+ +
Sbjct: 68 SNIGELELEYQAKF 81


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16500RTXTOXIND761e-17 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 76.4 bits (188), Expect = 1e-17
Identities = 49/290 (16%), Positives = 107/290 (36%), Gaps = 26/290 (8%)

Query: 55 ASLTVDEGDSIRAGQTLGELDRAPYENALLQAQANVSTAQAQYDLMMAGYRAEEIAQAAA 114
V E + +R + E + ++N Q + N+ +A+ ++A E
Sbjct: 175 YFQNVSEEEVLRLTSLIKE-QFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 115 AVKQAQAAYDYAQNFYQRQ--LGLRASSAISANDLENARSSRDQAQATLKSAQDKLRQYR 172
+ + + + L + N+L +S +Q ++ + SA+++ +
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 173 AGNRPQ---EIAQAKASLEQAQAALAQAKLDLHDTVLTAPSDGTLMTRAV-EPGTMLNAG 228
+ + ++ Q ++ LA+ + +V+ AP + V G ++
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 229 GTVLTLSLT-HPVWVRAYVDEKNLGQAQPGQEVLLYTDSRPDKPYH---GKIGFVSPSAE 284
T++ + + V A V K++G GQ ++ ++ P Y GK+ ++ A
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA- 412

Query: 285 FTPKTVETPDLRTDLVYRLRIVVTDADGA-------LRQGMPVTISFSHG 327
D R LV+ + I + + + L GM VT G
Sbjct: 413 -------IEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTG 455


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16490PF05272310.019 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.019
Identities = 11/27 (40%), Positives = 14/27 (51%)

Query: 30 IRAGYVTGLVGPDGAGKTTLMRMLAGL 56
+ Y L G G GK+TL+ L GL
Sbjct: 593 CKFDYSVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_16470ABC2TRNSPORT452e-07 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 45.3 bits (107), Expect = 2e-07
Identities = 31/137 (22%), Positives = 56/137 (40%), Gaps = 1/137 (0%)

Query: 197 AREREQGTLDQLLVSPLATWQIFVGKAVPALIVATLQATIVLAIGIWAYQIPFAGSLLLF 256
R Q T + +L + L I +G+ A A L + + + SLL
Sbjct: 92 GRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWL-SLLYA 150

Query: 257 YFTMVIYGLSLVGFGLLISSLCATQQQAFIGVFVFMMPAILLSGYVSPVENMPQWLQNLT 316
+ + GL+ G+++++L + + + P + LSG V PV+ +P Q
Sbjct: 151 LPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQTAA 210

Query: 317 WINPIRHFTDITKQIYL 333
P+ H D+ + I L
Sbjct: 211 RFLPLSHSIDLIRPIML 227


111KPHS_14550KPHS_14440N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_145501161.079033putative short-chain alcohol dehydrogenase
KPHS_14540-1171.314197hypothetical protein
KPHS_14530-1192.368705Xre family transcriptional regulator
KPHS_14520-2192.977858hypothetical protein
KPHS_14510-2183.693192carbon starvation protein
KPHS_14500-1184.530174hypothetical protein
KPHS_14490-1185.0004092,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
KPHS_14480-1185.5794822,3-dihydro-2,3-dihydroxybenzoate synthetase
KPHS_144700186.459117enterobactin synthase subunit E
KPHS_144601187.353198isochorismate synthase
KPHS_144503218.450136iron-enterobactin transporter periplasmic
KPHS_144404218.735094enterobactin exporter EntS
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14550DHBDHDRGNASE1355e-41 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 135 bits (341), Expect = 5e-41
Identities = 87/253 (34%), Positives = 130/253 (51%), Gaps = 15/253 (5%)

Query: 5 LTGKKALVTGASRGLGRAIALSLARAGADVVITYEKSVDKAQAVADEIKALGRYGEAVQA 64
+ GK A +TGA++G+G A+A +LA GA + + + +K + V +KA R+ EA A
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPA 64

Query: 65 DSASAQAIQDAVTHAARSLGGLDILVNNAGIARGGPLESMTLADIDALINVNIRGVVIAT 124
D + AI + R +G +DILVN AG+ R G + S++ + +A +VN GV A+
Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNAS 124

Query: 125 QEALVHMAD--GGRIINIGSCLANRVAMPGIAVYAMTKSALNALTRGLARDLGPRGITVN 182
+ +M D G I+ +GS A +A YA +K+A T+ L +L I N
Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRT-SMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 183 LVHPGPTNSDMN-----PEDGEQ------AEAQRQMIAVGHYGQPEDIAAAVTFLASPAA 231
+V PG T +DM E+G + E + I + +P DIA AV FL S A
Sbjct: 184 IVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243

Query: 232 GQISGTGLDVDGG 244
G I+ L VDGG
Sbjct: 244 GHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14510ACRIFLAVINRP310.014 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 31.3 bits (71), Expect = 0.014
Identities = 9/60 (15%), Positives = 25/60 (41%), Gaps = 1/60 (1%)

Query: 172 VIILAVLAMIVVKALTHSPWG-TYTVAFTIPLAIFMGIYIRYLRPGRIGEVSVIGLVMLV 230
++ ++ + + + A + W +V +PL I + L + ++GL+ +
Sbjct: 875 LVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTI 934


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14490DHBDHDRGNASE340e-121 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 340 bits (873), Expect = e-121
Identities = 109/265 (41%), Positives = 151/265 (56%), Gaps = 20/265 (7%)

Query: 1 MAALDFRGQTVWVTGAGKGIGYATALAFVEAGANVTGFD---------------LAFDGE 45
M A G+ ++TGA +GIG A A GA++ D A E
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 46 SYPFATETLDVADADQVREACSRLLANTERLDVLVNAAGILRMGATDQLSAEDWQQTFAV 105
++P DV D+ + E +R+ +D+LVN AG+LR G LS E+W+ TF+V
Sbjct: 61 AFP-----ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSV 115

Query: 106 NVGGAFNLFQQTMAQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALTVGLEL 165
N G FN + +R G+IVTV S+ A PR M+AY +SKAA +GLEL
Sbjct: 116 NSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLEL 175

Query: 166 AGSGVRCNLVSPGSTDTDMQRTLWVSDDAEQQRIRGFGEQFKLGIPLGKIARPQEIANTI 225
A +RCN+VSPGST+TDMQ +LW ++ +Q I+G E FK GIPL K+A+P +IA+ +
Sbjct: 176 AEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAV 235

Query: 226 LFLASSHASHITLQDIVVDGGSTLG 250
LFL S A HIT+ ++ VDGG+TLG
Sbjct: 236 LFLVSGQAGHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14480ISCHRISMTASE426e-154 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 426 bits (1096), Expect = e-154
Identities = 152/303 (50%), Positives = 201/303 (66%), Gaps = 20/303 (6%)

Query: 1 MAIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANI 60
MAIP +Q Y +P ASD+P NKV+W +P+RA LLIHDMQ YF++ + ++ + ++ ANI
Sbjct: 1 MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI 60

Query: 61 AALRDFCKQNGIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTV 120
L++ C Q GIPV YTAQP Q+ +DRALL D WGPGL P ++++I LAP++DD V
Sbjct: 61 RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV 120

Query: 121 LVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTTATDAFMRDIKPFFVADALAD 180
L KWRYSAF R+ L EM+++ GRDQLIITG+YAHIGC+ TA +AFM DIK FFV DA+AD
Sbjct: 121 LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD 180

Query: 181 FSREEHLMALKYVAGRSGRVVMTEELL--------PIPVSKA-----------ALRALIL 221
FS E+H MAL+Y AGR VMT+ LL + + A +R I
Sbjct: 181 FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA 240

Query: 222 PLLDESDEPLD-DENLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLTR 280
LL E+ E + E+L+D GLDSVR+M L +WR+ ++ FV LA+ PTI+ W LLT
Sbjct: 241 ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLTT 300

Query: 281 EVQ 283
Q
Sbjct: 301 RSQ 303


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14450FERRIBNDNGPP511e-09 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 51.5 bits (123), Expect = 1e-09
Identities = 59/288 (20%), Positives = 99/288 (34%), Gaps = 31/288 (10%)

Query: 40 HTLPSQPLRIVSTSVTLTGSLLAIDAPVVASGATTPNNRVADSQGFLRQWSEVAKARKLA 99
H P RIV+ LLA+ VAD+ + SE +
Sbjct: 29 HAAAIDPNRIVALEWLPVELLLALGIVPYG---------VADTINYRLWVSEPPLPDSV- 78

Query: 100 RLYIG---EPSAEAVAAQMPDLILVSATGGDSALPLYDQLKTIAPTLVINYDDKS----- 151
+ +G EP+ E + P ++ SA G P + L IAP N+ D
Sbjct: 79 -IDVGLRTEPNLELLTEMKPSFMVWSAGYG----PSPEMLARIAPGRGFNFSDGKQPLAM 133

Query: 152 WQTLLTQLGQITGHEQQASARIADFNKQLVSLKEKMKLPPQPVTALVYTAAAHSANIWTP 211
+ LT++ + + A +A + + S+K + L ++ P
Sbjct: 134 ARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGP 193

Query: 212 ESAQGQMLQQLGFSLATLPGGLPASHSQGKRHDIVQLGGENLAAGLNGQSLFLFAGDQKD 271
S ++L + G A + + + LAA + L + KD
Sbjct: 194 NSLFQEILDEYGIP--------NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKD 245

Query: 272 ADAIYANPLLAHLPAVAGKRVYPLGTETFRLDYYSALLVLQRLSSLFG 319
DA+ A PL +P V R + F SA+ ++ L + G
Sbjct: 246 MDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVLDNAIG 293


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_14440TCRTETB354e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 35.2 bits (81), Expect = 4e-04
Identities = 39/187 (20%), Positives = 71/187 (37%), Gaps = 8/187 (4%)

Query: 24 IARFISILSLGLLGVAIPVQIQMMTHSTWQVGLSVTLTGASMFVGLMVGGVLADRYKRKR 83
I F S+L+ +L V++P T + +G V G L+D+ KR
Sbjct: 21 ILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKR 80

Query: 84 LILLARGTCGVGFVGLCLNALLPEPSLAAIYLLGIWDGFFASLGVTALLAATPALVGREN 143
L+L G V + + A ++ G F +L ++ + +EN
Sbjct: 81 LLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPAL----VMVVVARYIPKEN 136

Query: 144 LMQAGAITMLTVRLGSVISPMIGGLLLATGGVAWNFGLAAAGTFITTLTLLRLPQLPPPP 203
+A + V +G + P IGG++ + W++ L IT +T+ L +L
Sbjct: 137 RGKAFGLIGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIP--MITIITVPFLMKLLKKE 192

Query: 204 QPREHPL 210
+
Sbjct: 193 VRIKGHF 199


112KPHS_13780KPHS_13740N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_13780013-0.359613putative inner membrane protein
KPHS_137701163.352761putative inner membrane protein
KPHS_137600152.911723putative permease
KPHS_137500142.303377hypothetical protein
KPHS_13740011-1.380495major facilitator superfamily transporter MFS_1
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13780HTHFIS691e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 69.5 bits (170), Expect = 1e-14
Identities = 34/136 (25%), Positives = 66/136 (48%), Gaps = 10/136 (7%)

Query: 2 LFIDEVHRLPPEGQEKLFHFMDNGSWRRLGESADERSATVRLIFASTEDLEK-----HFL 56
LF+DE+ +P + Q +L + G + +G + VR++ A+ +DL++ F
Sbjct: 235 LFLDEIGDMPMDAQTRLLRVLQQGEYTTVG-GRTPIRSDVRIVAATNKDLKQSINQGLFR 293

Query: 57 ATFIRRIPVI-VKILPIAERGQFERLAFIHHFFRREAQRLNHD-LALDGEIVSQLMRETL 114
R+ V+ +++ P+ +R E + + F ++A++ D D E + +
Sbjct: 294 EDLYYRLNVVPLRLPPLRDRA--EDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPW 351

Query: 115 EGNVGGLENLIRNICA 130
GNV LENL+R + A
Sbjct: 352 PGNVRELENLVRRLTA 367


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13770HTHFIS260.047 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.3 bits (58), Expect = 0.047
Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 4/51 (7%)

Query: 89 QALCADRQDSLAQLIGAQGSLQEALRQCKAAISYPGAGLPLLLRGPTGTGK 139
L D QD + L+G ++QE R + L L++ G +GTGK
Sbjct: 127 SKLEDDSQDGMP-LVGRSAAMQEIYRVLARLMQTD---LTLMITGESGTGK 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13750CARBMTKINASE300.012 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 29.8 bits (67), Expect = 0.012
Identities = 18/87 (20%), Positives = 32/87 (36%), Gaps = 10/87 (11%)

Query: 199 AMAEHRGDPAWENKLARFFAASSEFEALWHQRYEVRGVENQIKHFNHPQLGRFSLQQMYW 258
A+ + ++E + + + + + YEV I H N PQ+G L
Sbjct: 13 ALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEV-----VITHGNGPQVGSLLLHMDAG 67

Query: 259 YSAPRNGSRLLVYLPMDEAGEQALAWL 285
+ + PMD AG + W+
Sbjct: 68 QATYGIPA-----QPMDVAGAMSQGWI 89


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_13740TCRTETB1044e-26 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 104 bits (260), Expect = 4e-26
Identities = 77/409 (18%), Positives = 161/409 (39%), Gaps = 29/409 (7%)

Query: 21 MLPLIDTSITNVALDAITHTLAASATQLELIVALYGVAFAVCLAMGSKLGDNYGRRRLFM 80
+++ + NV+L I + + + + F++ A+ KL D G +RL +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 81 WGVALFGIASLLCGMANSIGALL-AARTLQGAGAALIVPQILATLHVTLKGPAH-ARAIS 138
+G+ + S++ + +S +LL AR +QGAGAA P ++ + + +A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAF-PALVMVVVARYIPKENRGKAFG 142

Query: 139 LYGGIGGIAFIVGQMGGGWLVSADIAGLGWRNAFFINVPICLLVLALSRRYVPETRRETP 198
L G I + VG GG + + W ++ + +P+ ++ + +
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHY----IHW--SYLLLIPMITIITVPFLMKLLKKEVRIK 196

Query: 199 SRIDWQGTLYL-ALILCCLLFPMALGPELHWPLWLQLMLVAVLPLLFAMRQSALRQQQRG 257
D +G + + I+ +LF + L++ + L+F ++ ++
Sbjct: 197 GHFDIKGIILMSVGIVFFMLFTTSY-------SISFLIVSVLSFLIF------VKHIRKV 243

Query: 258 DHPLLPPRLLQLTSIRFGMAIALLFFGAWSGFMFCMALTMQEGLGMAPWQSGNSFIALG- 316
P + P L + G+ + FG +GF+ + M++ ++ + G+ I G
Sbjct: 244 TDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGT 303

Query: 317 VAYFISALYAPRLIARYSMGRILLTGLAVQIAGLLLLCATFSRFGVATNALTLVPATALI 376
++ I L+ R +L G+ L F + T + + +
Sbjct: 304 MSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTAS-----FLLETTSWFMTIIIVFV 358

Query: 377 GYGQALIVNSFYRIGMRDISASDAGAGSAILSTLQQATLGLGPAILGSL 425
G + I + +AGAG ++L+ + G G AI+G L
Sbjct: 359 LGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGL 407


113KPHS_11910KPHS_11880N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_11910013-2.734383potassium efflux protein KefA
KPHS_11900117-3.846914DNA-binding transcriptional repressor AcrR
KPHS_11890117-3.520668acridine efflux pump
KPHS_11880216-3.917750acridine efflux pump
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11910GPOSANCHOR474e-07 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 46.6 bits (110), Expect = 4e-07
Identities = 42/282 (14%), Positives = 95/282 (33%), Gaps = 4/282 (1%)

Query: 31 RAADLPDRAEVQSQLNTLNKQKELTPQDKLVQQDLTQTLETLDKIERIKSETAQLRQQVE 90
+ +DL + N +EL+ + ++++ E KI+ +++ A L + +E
Sbjct: 72 KNSDLSFNNKALKDHND-ELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALE 130

Query: 91 QAPAKLRQAVESLNNLSDVPNDDATRKTLSTLSLRQLESRVTQTLDDLQNAQNDLATYNS 150
A + L A RK +L + T ++ + + A +
Sbjct: 131 GAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEA 190

Query: 151 QLVSLQTQPERVQNAMFNASQQLQQIRNRLNGTSVGD---ETLRPTQQVLLQAQQALLNA 207
+ L+ E N S +++ + + E A A +
Sbjct: 191 RQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKT 250

Query: 208 QIEQQRKSLEGNTILQDTLQKQRDYVTAWSNRLEHQLQLLQEAVNSKRLTLTEKTAQEAV 267
++ L+ L+ ++ TA S +++ K + A
Sbjct: 251 LEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNAN 310

Query: 268 TPDETARIQANPLVKQELDINHQLSEKLIQATENGNQLVQRN 309
+ A+ K++L+ HQ E+ + +E Q ++R+
Sbjct: 311 RQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRD 352


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11900HTHTETR1852e-61 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 185 bits (470), Expect = 2e-61
Identities = 170/213 (79%), Positives = 194/213 (91%)

Query: 1 MARKTKQQARETRQLILDVALRLFSQQGVSSTSLATIAKAAGVTRGAIYWHFKNKSDLFN 60
MARKTKQ+A+ETRQ ILDVALRLFSQQGVSSTSL IAKAAGVTRGAIYWHFK+KSDLF+
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EIWELSDASISDLEIEYRAKFPNDPLSVIREILVYVLEATVTEERRRLMMEIIYHKCEFV 120
EIWELS+++I +LE+EY+AKFP DPLSV+REIL++VLE+TVTEERRRL+MEII+HKCEFV
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 121 GEMTVVQQAQRQLSLASYERIEQTLKECIAAKLLPANLLTRRAAVLMRSYLSGLMENWLF 180
GEM VVQQAQR L L SY+RIEQTLK CI AK+LPA+L+TRRAA++MR Y+SGLMENWLF
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 181 APDSFDLHAEARDYVAILLEMYQFCPTLRGPES 213
AP SFDL EARDYVAILLEMY CPTLR P +
Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPAT 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11890RTXTOXIND424e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 4e-06
Identities = 29/210 (13%), Positives = 74/210 (35%), Gaps = 19/210 (9%)

Query: 100 TYQASYDSAKGDLAKAQAAANMDQLTVKRYQKLLGTKYISQQDYDTAVATA-QQSNAAVV 158
+ Y A +L ++ + + ++ Q + + +Q+ +
Sbjct: 256 EQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLV---TQLFKNEILDKLRQTTDNIG 312

Query: 159 AAKAAVETARINLAYTKVTSPISGRIGK-STVTEGALVQNGQTTALATVQQLDPIYVDVT 217
+ + + +P+S ++ + TEG +V +T + V + D + V
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETL-MVIVPEDDTLEVTAL 371

Query: 218 QSSNDFLRLKQEL-ADGRLKQENGK------AKVELVTNDGLKYPQSGTLEFSDVTVDQT 270
+ D + A +++ KV+ + D ++ + G + +++++
Sbjct: 372 VQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEEN 431

Query: 271 TGSITLRAIFPNPDHTLLPGMFVRARLEEG 300
S + I L GM V A ++ G
Sbjct: 432 CLSTGNKNIP------LSSGMAVTAEIKTG 455



Score = 29.0 bits (65), Expect = 0.039
Identities = 17/78 (21%), Positives = 33/78 (42%), Gaps = 3/78 (3%)

Query: 48 APLQITTELPGR-TSAYRIAEVRPQVSGIILKRNFV-EGSDIQAGVSLYQIDPATYQASY 105
++I G+ T + R E++P + I+ K V EG ++ G L ++ +A
Sbjct: 78 GQVEIVATANGKLTHSGRSKEIKPIENSIV-KEIIVKEGESVRKGDVLLKLTALGAEADT 136

Query: 106 DSAKGDLAKAQAAANMDQ 123
+ L +A+ Q
Sbjct: 137 LKTQSSLLQARLEQTRYQ 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11880ACRIFLAVINRP13650.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1365 bits (3535), Expect = 0.0
Identities = 805/1032 (78%), Positives = 911/1032 (88%)

Query: 1 MPNFFIDRPIFAWVIAIIIMLAGGLSILKLPVAQYPTIAPPAISITAMYPGADAETVQNT 60
M NFFI RPIFAWV+AII+M+AG L+IL+LPVAQYPTIAPPA+S++A YPGADA+TVQ+T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDHLMYMSSNGDSTGTATITLTFESGTDPDIAQVQVQNKLALATPLLPQ 120
VTQVIEQNMNGID+LMYMSS DS G+ TITLTF+SGTDPDIAQVQVQNKL LATPLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGISVEKASSSFLMVVGVINTNGTMNQDDISDYVAANMKDPISRTSGVGDVQLFGS 180
EVQQQGISVEK+SSS+LMV G ++ N QDDISDYVA+N+KD +SR +GVGDVQLFG+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWMDPNKLNNFQLTPVDVISALKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRL 240
QYAMRIW+D + LN ++LTPVDVI+ LK QN Q+AAGQLGGTP + GQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 TNTEEFGNILLKVNQDGSQVRLRDVAKIELGGESYDVVAKFNGQPASGLGIKLATGANAL 300
N EEFG + L+VN DGS VRL+DVA++ELGGE+Y+V+A+ NG+PA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTANAIRAELAKMEPFFPSGMKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ 360
DTA AI+A+LA+++PFFP GMK++YPYDTTPFV++SIHEVVKTL EAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
N RATLIPTIAVPVVLLGTFA+LAAFG+SINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 AEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480
E+ LPPKEAT KSM QIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATMLKPIQKGSHGATTGFFGWFNRMFDKSTHHYTDSVGNILRSTGRY 540
SVLVALILTPALCAT+LKP+ H GFFGWFN FD S +HYT+SVG IL STGRY
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 541 LVLYLIIVVGMAWLFVRLPSSFLPDEDQGVFLSMAQLPAGATQERTQKVLDEMTNYYLTK 600
L++Y +IV GM LF+RLPSSFLP+EDQGVFL+M QLPAGATQERTQKVLD++T+YYL
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 601 EKDNVESVFAVNGFGFAGRGQNTGIAFVSLKDWSQRPGEENKVEAITARAMGYFSQIKDA 660
EK NVESVF VNGF F+G+ QN G+AFVSLK W +R G+EN EA+ RA +I+D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 661 MVFAFNLPAIVELGTATGFDFELIDQGGLGHEKLTQARNQLFGMVAQHPDVLTGVRPNGL 720
V FN+PAIVELGTATGFDFELIDQ GLGH+ LTQARNQL GM AQHP L VRPNGL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 721 EDTPQFKIDIDQEKAQALGVSISDINTTLGAAWGGSYVNDFIDRGRVKKVYIMSEAKYRM 780
EDT QFK+++DQEKAQALGVS+SDIN T+ A GG+YVNDFIDRGRVKK+Y+ ++AK+RM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 781 LPEDIGKWYVRGSDGQMVPFSAFSTSRWEYGSPRLERYNGLPSLEILGQAAPGKSTGEAM 840
LPED+ K YVR ++G+MVPFSAF+TS W YGSPRLERYNGLPS+EI G+AAPG S+G+AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 841 ALMEELAGKLPSGIGYDWTGMSYQERLSGNQAPALYAISLIVVFLCLAALYESWSIPFSV 900
ALME LA KLP+GIGYDWTGMSYQERLSGNQAPAL AIS +VVFLCLAALYESWSIP SV
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 901 MLVVPLGVVGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGLI 960
MLVVPLG+VG LLAAT NDVYF VGLLTTIGLSAKNAILIVEFAKDLMEKEGKG++
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 961 EATLEAVRMRLRPILMTSLAFILGVMPLVISSGAGSGAQNAVGTGVMGGMVTATILAIFF 1020
EATL AVRMRLRPILMTSLAFILGV+PL IS+GAGSGAQNAVG GVMGGMV+AT+LAIFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1021 VPVFFVVVRRRF 1032
VPVFFVV+RR F
Sbjct: 1021 VPVFFVVIRRCF 1032


114KPHS_11030KPHS_10990N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_11030221-0.965938nucleoside channel, receptor of phage T6 and
KPHS_11020218-0.836587DeoR family transcriptional regulator
KPHS_11010219-0.788506hypothetical protein
KPHS_11000219-1.119385preprotein translocase subunit SecF
KPHS_10990217-0.696689preprotein translocase subunit SecD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11030CHANNELTSX5370.0 Nucleoside-specific channel-forming protein Tsx signa...
		>CHANNELTSX#Nucleoside-specific channel-forming protein Tsx

signature.
Length = 294

Score = 537 bits (1384), Expect = 0.0
Identities = 294/294 (100%), Positives = 294/294 (100%)

Query: 1 MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60
MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE
Sbjct: 1 MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60

Query: 61 YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP 120
YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP
Sbjct: 61 YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP 120

Query: 121 FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN 180
FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN
Sbjct: 121 FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN 180

Query: 181 EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH 240
EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH
Sbjct: 181 EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH 240

Query: 241 ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF 294
ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF
Sbjct: 241 ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11020ARGREPRESSOR336e-04 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 32.5 bits (74), Expect = 6e-04
Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 7/66 (10%)

Query: 3 RRADRLFQIVQILRGRRLTT-----AALLAERLGVSERTVYRDIRDLSLSGVPVEGEAGS 57
+ R +I +I+ + T L + V++ TV RDI++L L V V GS
Sbjct: 2 NKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKELHL--VKVPTNNGS 59

Query: 58 GYRLLA 63
L
Sbjct: 60 YKYSLP 65


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_11000SECFTRNLCASE342e-120 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 342 bits (880), Expect = e-120
Identities = 101/308 (32%), Positives = 172/308 (55%), Gaps = 12/308 (3%)

Query: 18 DFMRWDYWAFGISGFLLIVSIAIIGVRGFNWGLDFTGGTVIEITLEKPVDLDQMRDSLQK 77
DF RW + FG + ++I S+ + V G N+G+DF GGT I +D+ R +L+
Sbjct: 15 DFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAALEP 74

Query: 78 AGFEEPQVQNFGSSR------DIMVRMPPVHDANGSQELGSKVVTVINE------STSQN 125
+ + M+R+ D G++ G++ ++N+ +
Sbjct: 75 LELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAVDPA 134

Query: 126 AAVKRIEFVGPSVGADLAQTGALALIAALVCILIYVGFRFEWRLAAGVVIALAHDVVITM 185
+ E VGP V +L T +L+AA V I+ Y+ RFEW+ A G V+AL HDV++T+
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 186 GVLSLFHIEIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRGTPYEIFNVSLTQTL 245
G+ ++ ++ DLT VA+L+++ GYS+ND++VV DR+REN K + ++ N+S+ +TL
Sbjct: 195 GLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNETL 254

Query: 246 HRTLITSGTTLMVILMLFLFGGPILEGFSLTMLIGVSIGTASSIYVASALALKLGMKREH 305
RT++T TTL+ ++ + ++GG ++ GF M+ GV GT SS+YVA + L +G+ R
Sbjct: 255 SRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIVLFIGLDRNK 314

Query: 306 LIQQKVEK 313
+ +K
Sbjct: 315 EKKDPSDK 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10990SECFTRNLCASE695e-15 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 69.5 bits (170), Expect = 5e-15
Identities = 37/183 (20%), Positives = 88/183 (48%), Gaps = 5/183 (2%)

Query: 422 IQIVEERTIGPTLGMQNIKQGLEACLAGLVVSILFMIL-FYKKFGLIATSALIANLILIV 480
++I ++GP + + + + + LA VV + ++ + F +F L A AL+ +++L V
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 481 GIMSLIPGATLTMPGIAGIVLTLAVAVDANVLINERIKEEL--SNGRTVQQAIDEGYRGA 538
G+ +++ + +A ++ +++ V++ +R++E L ++ ++
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 539 FSSIFDANVTTLIKVIILYAVGTGAIKGFAITTGIGIATSMFTAIVGTRAIVNLLYGGKR 598
S +TTL+ ++ + G I+GF G+ T ++++ + IV L G R
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIV-LFIGLDR 312

Query: 599 VKK 601
K+
Sbjct: 313 NKE 315


115KPHS_10860KPHS_10750N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_10860-1152.077094hypothetical protein
KPHS_10850-1121.672725putative transport protein
KPHS_10840-2131.491922putative cysteine hydrolase
KPHS_10830-2111.399231putative LysR-family transcriptional regulator
KPHS_10820-2141.815920maltodextrin glucosidase
KPHS_108101122.492690proline permease
KPHS_108001123.059739branched chain amino acid transport system II
KPHS_107903122.786443positive and negative sensor protein for pho
KPHS_107804112.925717transcriptional regulator PhoB
KPHS_107703113.031368ATP-dependent dsDNA exonuclease
KPHS_107604112.644937ATP-dependent dsDNA exonuclease
KPHS_10750-2150.482508putative NAGC-like transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10860UREASE300.033 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 30.1 bits (68), Expect = 0.033
Identities = 22/76 (28%), Positives = 31/76 (40%), Gaps = 14/76 (18%)

Query: 4 TATLILTHGQIHTLDRANPLAEAVAIADGKIVATGS------HDRIMSFAAEGTQIVDLK 57
T LIL H I D + + DG+I A G + GT+++ +
Sbjct: 73 TNALILDHWGIVKAD--------IGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGE 124

Query: 58 GHTVIPGLNDSHLHLI 73
G V G DSH+H I
Sbjct: 125 GKIVTAGGMDSHIHFI 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10840ISCHRISMTASE366e-05 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 36.1 bits (83), Expect = 6e-05
Identities = 35/186 (18%), Positives = 67/186 (36%), Gaps = 21/186 (11%)

Query: 7 LDPTNSALIFIDHQPQM--SFGVANIDRQTLKNNTVALAKAGKIFNVPVIYT------SV 58
DP + L+ D Q +F L N L +PV+YT +
Sbjct: 26 PDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCVQLGIPVVYTAQPGSQNP 85

Query: 59 ETKSFSGYIW-PELLAVHPDVKPIERTS-------MNSWEDDAF-----VAAVKATGRKK 105
+ ++ W P L + + K I + + W AF + ++ GR +
Sbjct: 86 DDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLVLTKWRYSAFKRTNLLEMMRKEGRDQ 145

Query: 106 LVISALWTEVCLTFPALMALEAGYEVYVVTDTSGGTSVDAHERSIDRMVQAGAVPVTWQQ 165
L+I+ ++ + A A + + V D S++ H+ +++ A V
Sbjct: 146 LIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFSLEKHQMALEYAAGRCAFTVMTDS 205

Query: 166 VLLEYQ 171
+L + Q
Sbjct: 206 LLDQLQ 211


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10810TCRTETA290.030 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 29.4 bits (66), Expect = 0.030
Identities = 17/75 (22%), Positives = 29/75 (38%)

Query: 357 FLVIASLATFATVWVWIMILLSQIAFRRRLSPEEVKALKFKVPGGVVTTVIGLLFLAFII 416
F A+L + ++ S RR L E + L +T V L+ + FI+
Sbjct: 163 FFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM 222

Query: 417 ALIGYHPDTRISLYV 431
L+G P ++
Sbjct: 223 QLVGQVPAALWVIFG 237


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10790PF06580310.006 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 31.4 bits (71), Expect = 0.006
Identities = 16/98 (16%), Positives = 30/98 (30%), Gaps = 25/98 (25%)

Query: 325 LVYNAVNH----TPPGTEIRVSWQRTPQGALFSVEDNGPGIAPEHIPRLTERFYRVDKAR 380
LV N + H P G +I + + VE+ G
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKN---------------- 306

Query: 381 SRQTGGSGLGLAIVKHAVNH---HDSRLEIDSTVGKGT 415
+G GL V+ + ++++++ GK
Sbjct: 307 --TKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVN 342


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10780HTHFIS965e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 95.7 bits (238), Expect = 5e-25
Identities = 34/149 (22%), Positives = 63/149 (42%), Gaps = 9/149 (6%)

Query: 4 RILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGLQ 63
ILV +D+A IR ++ L + G+ + + + DL++ D ++P +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 FIKLLKREAMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRI 123
+ +K+ D+PV++++A+ ++ E GA DY+ KPF EL+ I +
Sbjct: 65 LLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA-- 120

Query: 124 SPMAVEEVIEMQGLSLDPSSHRVMTGDSP 152
E L D + G S
Sbjct: 121 -----EPKRRPSKLEDDSQDGMPLVGRSA 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10760RTXTOXIND374e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 37.1 bits (86), Expect = 4e-04
Identities = 26/207 (12%), Positives = 54/207 (26%), Gaps = 23/207 (11%)

Query: 196 ARHALEKFEAQAAGIVLLTEAQQQALQESLQVLTDEEKALLAQQQSQQQQLQWLTRRDEL 255
A K ++ L + + Q L S+++ E L + Q + + R L
Sbjct: 132 AEADTLKTQSSLL-QARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSL 190

Query: 256 AQQQQQAATRQQ-QARQALADAAPALAKLE------------LAQPAAQLRPLWERQQEQ 302
++Q Q+ Q L + L +Q
Sbjct: 191 IKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIA 250

Query: 303 TAGLAQTRQRISEVNARLLASTALRARIRQGALRAQQQRQAELADLAQWLAAHERFRLWG 362
+ + + E L + +I L A+++ Q L
Sbjct: 251 KHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQ---------LVTQLFKNEIL 301

Query: 363 QEIAGWRAQFSQLTRDKQQLTAQSTRL 389
++ LT + + +
Sbjct: 302 DKLRQTTDNIGLLTLELAKNEERQQAS 328



Score = 34.8 bits (80), Expect = 0.002
Identities = 26/204 (12%), Positives = 64/204 (31%), Gaps = 23/204 (11%)

Query: 308 QTRQRISEVNARLLASTALRARIRQGALRAQQQRQAELADLAQWLAAHERFRLWGQEIAG 367
+ + LL + + R + + + + EL + + + +
Sbjct: 131 GAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSL 190

Query: 368 WRAQFSQLTRDKQQLTAQSTRLAALRQKLATLPASPLTLSADDVAAAIEQQTQS--RPL- 424
+ QFS K Q L R + T+ A ++ + + +E+ L
Sbjct: 191 IKEQFSTWQNQKYQKELN---LDKKRAERLTVLA---RINRYENLSRVEKSRLDDFSSLL 244

Query: 425 ------RQRLLSLHEQHQLLRKRLRQNADSVQQAQAEQVKLNATLTLRREQYKDKNQHYL 478
+ +L ++ LR ++Q ++E + L + +K+
Sbjct: 245 HKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKN------ 298

Query: 479 DLKALCQREETIKDLESYRDRLEA 502
+ L + +T ++ L
Sbjct: 299 --EILDKLRQTTDNIGLLTLELAK 320



Score = 32.5 bits (74), Expect = 0.009
Identities = 37/294 (12%), Positives = 88/294 (29%), Gaps = 52/294 (17%)

Query: 418 QTQSRPLRQRLLSLHEQHQLLRKRLRQNADSVQQAQAEQVKLNATLTLRREQYKDKNQHY 477
+ + S Q +L + R + + S++ + ++KL + ++ +
Sbjct: 129 ALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLT 188

Query: 478 LDLKALCQREETIKDLESYRDRLEAGKPCPLCGACEHPAIEQYASLTLTDNQRRRDALEK 537
+K +++++ + L L + R +
Sbjct: 189 SLIKE---------QFSTWQNQKYQKE------------------LNLDKKRAERLTVLA 221

Query: 538 EVAALKEEGLLILGQVKALTQQLQRDTEAAGRLAEEEQALTKAWQETCASLHITRDIAQE 597
+ + + ++ + L + A + E+E +A E L + + ++
Sbjct: 222 RINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNE----LRVYKSQLEQ 277

Query: 598 INDWMQEQERYEQQLYQLSQRLMLQSQLNDQQALERQAEQQLAATRQGLESALQALALSL 657
I + + Q + QL + +L +L +LA + QA +
Sbjct: 278 IESEILSAKEEYQLVTQLFKNEIL-DKLRQTTDNIGLLTLELAKNEE----RQQASVIRA 332

Query: 658 PAEGTEAAWLHARESEFAQWQAQQTQHDAIQQQIAALRPLLETLPTSDETEVEA 711
P QQ + + L+ +P D EV A
Sbjct: 333 PVSVK----------------VQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTA 370



Score = 31.7 bits (72), Expect = 0.018
Identities = 40/206 (19%), Positives = 80/206 (38%), Gaps = 22/206 (10%)

Query: 675 AQWQAQQTQHDAIQQQIAALRPLLETLPTSDETEVEAESAIPD-------NWREIHEECL 727
A+ +TQ +Q ++ R + L S E E +PD + E+
Sbjct: 132 AEADTLKTQSSLLQARLEQTR--YQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTS 189

Query: 728 SLHSQLVAQQQQETQEKARLDQSQAQFTSALAASRFSDREAFLAALLDDETAQRLTQLKQ 787
+ Q Q Q+ Q++ LD+ +A+ + LA + + + RL
Sbjct: 190 LIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE-------KSRLDDFSS 242

Query: 788 TLEQQLQQAAALCEQATRQHEAHLALRPQGVDADVPTLQTQLHALAQRLRDNT-TRQGEI 846
L +Q A+ EQ + EA LR + + +++++ + + + T + EI
Sbjct: 243 LLHKQAIAKHAVLEQENKYVEAVNELRVY--KSQLEQIESEILSAKEEYQLVTQLFKNEI 300

Query: 847 RQQLRQDAESRQQQQALGQQIAEAAQ 872
+LRQ + L ++A+ +
Sbjct: 301 LDKLRQ---TTDNIGLLTLELAKNEE 323


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_10750ACETATEKNASE348e-04 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 33.6 bits (77), Expect = 8e-04
Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 1/40 (2%)

Query: 216 EGDEKAELALSRYEQRLAKSLAHVVNILDP-DVIVLGGGM 254
GD++A+LAL+ + R+ K++ + DVIV G+
Sbjct: 293 NGDKRAQLALNVFAYRVKKTIGSYAAAMGGVDVIVFTAGI 332


116KPHS_08880KPHS_08760N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_08880-2183.501374pullulanase-specific type II secretion system
KPHS_088700204.910557pullulanase D protein
KPHS_088603237.035855pullulanase E protein
KPHS_088502246.854398pullulanase-specific type II secretion system
KPHS_088402237.045815pullulanase G protein
KPHS_088305237.838964pullulanase H protein
KPHS_088205228.033470pullulanase I protein
KPHS_088105217.907920general secretion pathway protein J
KPHS_088007198.562810pullulanase-specific type II secretion system
KPHS_087907198.663010pullulanase L protein
KPHS_087804186.791777general secretion pathway protein M
KPHS_087702145.624890pullulanase N protein
KPHS_087601134.993799pullulanase Q protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08880BCTERIALGSPC2144e-71 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 214 bits (546), Expect = 4e-71
Identities = 100/266 (37%), Positives = 159/266 (59%), Gaps = 7/266 (2%)

Query: 17 KLLPQIVTLIILITAIPQLAKLTWRVVFPVSPEDISALPLTMPPAADPELKNVRPAFTLF 76
++ +I+ ++++ QLA + WR+ P ++ + + PA + FTLF
Sbjct: 12 SVIRRILFYLLMLLFCQQLAMIFWRIGLP---DNAPVSSVQITPAQARQQPVTLNDFTLF 68

Query: 77 GLAV-KNSPTPTDAASLNQVPVSSLKLRLAGLLASSNPARSIAIIEKGNQQVSLSTGDPL 135
G++ KN DA+ ++ +P S+L L L G++A + +RSIAII K N+Q S + +
Sbjct: 69 GVSPEKNKAGALDASQMSNLPPSTLNLSLTGVMAGDDDSRSIAIISKDNEQFSRGVNEEV 128

Query: 136 PGYDARIAAILPDRIIVNYQGRKEAILLFNDSRAPSPPPTAAGNPPLVKRLREQPQNILT 195
PGY+A+I +I PDR+++ YQGR E + L++ + S G + + +
Sbjct: 129 PGYNAKIVSIRPDRVVLQYQGRYEVLGLYSQEDSGSDGVP--GAQVNEQLQQRASTTMSD 186

Query: 196 YLSISPVLSGDKLLGYRLNPGKDASLFRQSGLQANDLAIALNGIDLRDQEQAQQALQNLA 255
Y+S SP+++ +KL GYRLNPG + F + GLQ ND+A+ALNG+DLRD EQA++A++ +A
Sbjct: 187 YVSFSPIMNDNKLQGYRLNPGPKSDSFYRVGLQDNDMAVALNGLDLRDAEQAKKAMERMA 246

Query: 256 DMTEITLTVEREGQRHDIAFAL-GDE 280
D+ TLTVER+GQR DI GDE
Sbjct: 247 DVHNFTLTVERDGQRQDIYMEFGGDE 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08870BCTERIALGSPD8380.0 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 838 bits (2167), Expect = 0.0
Identities = 606/646 (93%), Positives = 631/646 (97%)

Query: 10 ALLILTPLLFSPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML 69
LLI LLF PAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML
Sbjct: 13 TLLIFAALLFRPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML 72

Query: 70 NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRAKDAKTSAVPVASAAAPGEGDEVVTRVV 129
NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVR+KDAKT+AVPVAS AAPG GDEVVTRVV
Sbjct: 73 NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVV 132

Query: 130 PLTNVAARDLAPLLRQLNDNAGAGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR 189
PLTNVAARDLAPLLRQLNDNAG GSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR
Sbjct: 133 PLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDR 192

Query: 190 SVVTVPLSWASAAEVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRI 249
SVVTVPLSWASAA+VVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRI
Sbjct: 193 SVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRI 252

Query: 250 IAMIKQLDRQQAVQGNTKVIYLKYAKAADLVEVLTGISSSLQSDKQSARPVAAIDKNIII 309
IAMIKQLDRQQA QGNTKVIYLKYAKA+DLVEVLTGISS++QS+KQ+A+PVAA+DKNIII
Sbjct: 253 IAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKNIII 312

Query: 310 KAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKN 369
KAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKN
Sbjct: 313 KAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWANKN 372

Query: 370 AGMTQFTNSGLPISTAIAGANQYNKDGTISSSLASALGSFNGIAAGFYQGNWAMLLTALS 429
AGMTQFTNSGLPISTAIAGANQYNKDGT+SSSLASAL SFNGIAAGFYQGNWAMLLTALS
Sbjct: 373 AGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGNWAMLLTALS 432

Query: 430 SSTKNDILATPSIVTLDNMQATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKP 489
SSTKNDILATPSIVTLDNM+ATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKP
Sbjct: 433 SSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKP 492

Query: 490 QINEGDAVLLEIEQEVSSVADSASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKT 549
QINEGD+VLLEIEQEVSSVAD+ASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDK+
Sbjct: 493 QINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGSGETVVVGGLLDKS 552

Query: 550 VTDTADKVPLLGDIPVIGALFRSDSKKVSKRNLMLFIRPTIIRDRDEYRQASSGQYTAFN 609
V+DTADKVPLLGDIPVIGALFRS SKKVSKRNLMLFIRPT+IRDRDEYRQASSGQYTAFN
Sbjct: 553 VSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRDRDEYRQASSGQYTAFN 612

Query: 610 NAQTKQRGKESSEASLSNDLLHIYPQQETQAFRQVSAAIDAFNLGG 655
+AQ+KQRGKE+++A L+ DLL IYP+Q+T AFRQVSAAIDAFNLGG
Sbjct: 613 DAQSKQRGKENNDAMLNQDLLEIYPRQDTAAFRQVSAAIDAFNLGG 658


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08850BCTERIALGSPF5080.0 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 508 bits (1311), Expect = 0.0
Identities = 277/407 (68%), Positives = 334/407 (82%), Gaps = 4/407 (0%)

Query: 1 MALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLT-- 58
MA + YQALDAQGK RG Q+ADSAR ARQLLR++G + L V ++ L+
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 59 --RRTSAGDLALLTRQLATLVAAGIPLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSL 116
R S DLALLTRQLATLVAA +PLE+ALDAVA+Q EKP L LMA VRSKV+EGHSL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 117 AEAMRAYPACFDGLFYAMVAAGETSGHLDGVLNRLADYTEQRQQLRARLLQAMIYPIVLT 176
A+AM+ +P F+ L+ AMVAAGETSGHLD VLNRLADYTEQRQQ+R+R+ QAMIYP VLT
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 177 LVAISVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLA 236
+VAI+V++ILLS VVPKVVEQF+H+KQALP STR+LM +SD VR+ GPW+ L L +A
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 237 LRYLLRQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADV 296
R +LRQ RR+++ R LL LP+IGR+AR +N+ARYARTLSILNASAVPLL +MRIS DV
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 297 LSNAWARSQLAAASESVREGVSLHRALESTALFPPMMRYMIASGEQSGELTAMLERAAEN 356
+SN +AR +L+ A+++VREGVSLH+ALE TALFPPMMR+MIASGE+SGEL +MLERAA+N
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 357 QDRELSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNTLMS 403
QDRE S+Q+ +AL LFEPLLVV+MA +VLFIVLAILQPILQLNTLMS
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLMS 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08840BCTERIALGSPG2432e-86 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 243 bits (621), Expect = 2e-86
Identities = 98/140 (70%), Positives = 112/140 (80%)

Query: 1 MQRQRGFTLLEIMVVIVILGILASLVVPNLMGNKEKADRQKVVSDLVALEGALDMYKLDN 60
+QRGFTLLEIMVVIVI+G+LASLVVPNLMGNKEKAD+QK VSD+VALE ALDMYKLDN
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDN 63

Query: 61 SRYPNTEQGLQALVTAPAAEPHARNYPEGGYIRRLPQDPWGNEYQLLSPGQHGAIDVFSV 120
YP T QGL++LV AP P A NY + GYI+RLP DPWGN+Y L++PG+HGA D+ S
Sbjct: 64 HHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLLSA 123

Query: 121 GPDGMPDTNDDIGNWTLGKK 140
GPDG T DDI NW L KK
Sbjct: 124 GPDGEMGTEDDITNWGLSKK 143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08830BCTERIALGSPH1786e-60 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 178 bits (452), Expect = 6e-60
Identities = 99/164 (60%), Positives = 126/164 (76%)

Query: 1 MSQRGFTLLEMMLVLLLIGVSASMVLLAFPSARTQEATQILARFQAQLDFVRERGQQTGQ 60
M QRGFTLLEMML+LLL+GVSA MVLLAFP++R A Q LARF+AQL FV++RG QTGQ
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQ 60

Query: 61 LFGIIIHPERWQFMRLQPADDSAPAAADDRWGNAQWLPLQAGRVTTAETLPRARLTLRFP 120
FG+ +HP+RWQF+ L+ D + PA ADD W +WLPL+AGRV T+ ++ +L L F
Sbjct: 61 FFGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAGRVATSGSIAGGKLNLAFA 120

Query: 121 DGQAWTPGEQPDVLIFPGGEVTPFQLRIDAAMGINVDAQGDSQP 164
G+AWTPG+ PDVLIFPGGE+TPF+L + A GI +A+G+S P
Sbjct: 121 QGEAWTPGDNPDVLIFPGGEMTPFRLTLGEAPGIAFNARGESLP 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08820BCTERIALGSPG322e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.2 bits (73), Expect = 2e-04
Identities = 22/99 (22%), Positives = 36/99 (36%), Gaps = 8/99 (8%)

Query: 1 MKREAGMTLIEVMVALVIF-ALAGLAV---MQSTLQQTRQLGRMEEKILASWLADNQLVQ 56
++ G TL+E+MV +VI LA L V M + + +Q + L + L +L
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDN 63

Query: 57 LRLEKRWPALS--WSETTVEAAGTRWFVRWQGVETALPQ 93
L T+ + +G LP
Sbjct: 64 HHYPTTNQGLESLVEAPTLPPLAANY--NKEGYIKRLPA 100


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08810BCTERIALGSPG334e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 32.6 bits (74), Expect = 4e-04
Identities = 20/61 (32%), Positives = 34/61 (55%), Gaps = 5/61 (8%)

Query: 6 RGFTLIETLLALAILAVLSAAAV-MVLQNVIRADGLTREKSQ-QIAALQRAFRQIADDVT 63
RGFTL+E ++ + I+ VL++ V ++ N +AD ++K+ I AL+ A D
Sbjct: 8 RGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKAD---KQKAVSDIVALENALDMYKLDNH 64

Query: 64 H 64
H
Sbjct: 65 H 65


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_08760PREPILNPTASE2712e-93 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 271 bits (694), Expect = 2e-93
Identities = 138/277 (49%), Positives = 167/277 (60%), Gaps = 15/277 (5%)

Query: 1 MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRML---------------TQTA 45
M L L+ P++++ + LF L +GSF NVVI+RLP ML +
Sbjct: 1 MALLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGV 60

Query: 46 DDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWLGRRARCCQAPIAWSYPLTELATGLL 105
D+ L P S CP C PI +NIPLLS+LWL R R CQAPI+ YPL EL T LL
Sbjct: 61 DEPPYNLMVPRSCCPHCNHPITALENIPLLSWLWLRGRCRGCQAPISARYPLVELLTALL 120

Query: 106 FILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLNEVYIA 165
+ LAPG L+L L+ L ID LLPD+LTLPLLW GLLFNL +++
Sbjct: 121 SVAVAMTLAPGWGTLAALLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVS 180

Query: 166 LPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASA 225
L DAV GAMAGYL LWS+YW F+LLTGKE +GYGDFKLLAALGAW GWQ LP VLLL+S
Sbjct: 181 LGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL 240

Query: 226 SGLVWTLLQRLWTRQSLQQPLAFGPWLALAGGSIFLW 262
G + L +P+ FGP+LA+AG LW
Sbjct: 241 VGAFMGIGLILLRNHHQSKPIPFGPYLAIAGWIALLW 277


117KPHS_07890KPHS_07820N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_07890012-4.288120MFS family transporter
KPHS_07880-115-4.1060343-dehydroquinate dehydratase
KPHS_07870013-2.444848putative LysR-family transcriptional regulator
KPHS_07860110-0.647849major facilitator superfamily permease
KPHS_078503122.425831sugar efflux protein
KPHS_078403133.011677hypothetical protein
KPHS_078304133.249677hypothetical protein
KPHS_078203143.297669transcriptional regulator SgrR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07890TCRTETA393e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 38.7 bits (90), Expect = 3e-05
Identities = 57/343 (16%), Positives = 112/343 (32%), Gaps = 8/343 (2%)

Query: 60 VTGFLSDRFGRKPFIYLGILSYLIFFVGILLTKNIYLAYVFGIMAGLANSFLDSGTYPAL 119
V G LSDRFGR+P + + + + + + +++ Y+ I+AG+ +
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA 121

Query: 120 MESFPHSASRANVLIKAFVSAGQFLLPFIISFLIWANLWFGWSFVIAAALFVLSGIYLLK 179
+ +R + A G P + + F AAAL L+ +
Sbjct: 122 DITDGDERARHFGFMSACFGFGMVAGPVLGGLM--GGFSPHAPFFAAAALNGLNFLTGCF 179

Query: 180 MPFPDSQAAKKEEAPTAQAETAVRPQANK-LDMVIFTLYGYIGMATFYLVSQWL-AQYGQ 237
+ P+S ++ + + + +V + + M V L +G+
Sbjct: 180 L-LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGE 238

Query: 238 FVVGL-PYASAIKLLSIYTVGSLVCVFVTAAFVKEVFSSAIAMIIYTGLSMISLLLVCLF 296
I L + + SL +T + M+ +LL
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFAT 298

Query: 297 PTPMMVTGFAFVIGFAAAGGVLQLGATIMAMSFPNGKGKATGIFYTAGSIASFTIPLITA 356
M + LQ A + +G+ G S+ S PL+
Sbjct: 299 RGWMAFPIMVLLASGGIGMPALQ--AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFT 356

Query: 357 KLSQISIASIMWFDFLIAVIGFVIALYIGYRQLQARAAQKVSR 399
+ SI + + ++ +++ L R L + A Q+ R
Sbjct: 357 AIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR 399


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07860TCRTETA613e-12 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 61.0 bits (148), Expect = 3e-12
Identities = 71/415 (17%), Positives = 132/415 (31%), Gaps = 46/415 (11%)

Query: 30 LSVGTMINYLDRTILGI---VAPQLSKEIHID---PAMMGIIFSAFAWTYALAQIPGGMF 83
L V LD +G+ V P L +++ A GI+ + +A G
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 84 LDRFGNKVTYALSIFFWSLFTLLQSFTLGLKSLLLLRLGLGVSEAPCFPANSRIVSTWFP 143
DRFG + +S+ ++ + + L L + R+ G++ A ++
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGAT-GAVAGAYIADITD 125

Query: 144 QHERARA----TATYTVGEYIGLAAFSPLLFLILEHHGWRTLFFLTGGLGILFTLVWWRF 199
ERAR +A + G G P+L ++ FF L L L
Sbjct: 126 GDERARHFGFMSACFGFGMVAG-----PVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFL 180

Query: 200 YHEPHESRTANQAELEYIGASSINNKIQNVPFNWRDARRLLGCRQILGASLGQFAGNTTL 259
E H+ +N F W ++ + +
Sbjct: 181 LPESHKGERRPLRREA------LNPLAS---FRWARGMTVVAALMAVFFIMQLVGQ---- 227

Query: 260 VFFLTWFPSYLANERHLPWLHVGFFATWPFLAAAIGILFGGWISDRLLKRTGSVNISRKL 319
+ + + H +G + L I+ + R G
Sbjct: 228 -VPAALWVIFGEDRFHWDATTIGISLA---AFGILHSLAQAMITGPVAARLGERRA---- 279

Query: 320 PIISGLLLSSC--IIAANWVSANSTVIIIMSVAFFGQGMVGLGWTLISDIAPENMAGLTG 377
++ G++ I+ A I++ +A G GM L ++S E G
Sbjct: 280 -LMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQ-AMLSRQVDEERQGQLQ 337

Query: 378 GIFNFCANMASIIAPLIIGVIISATGNFFYALIYVGLTALIGVIAYIFIIGDIKR 432
G ++ SI+ PL+ I +A+ + G + G Y+ + ++R
Sbjct: 338 GSLAALTSLTSIVGPLLFTAIYAAS-----ITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07850TCRTETA330.002 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.9 bits (75), Expect = 0.002
Identities = 41/187 (21%), Positives = 67/187 (35%), Gaps = 17/187 (9%)

Query: 16 AAFMLVAFMMGVAGALQAPTLSLFLSREVGAQPFWVGLFYTVNAIAGILVSLWLAKRSDS 75
AA M V F+M + G + A +F +G+ I L + +
Sbjct: 213 AALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAA 272

Query: 76 RGDRRRLIMFCCLMAVGNALLFAFNRHYLTLITCGVMLASIANAAMPQLFALAREYADSS 135
R RR +M + +L AF V+LAS MP L A+ D
Sbjct: 273 RLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGG-IGMPALQAMLSRQVDEE 331

Query: 136 AREVVMFSSVMRAQLSLAWVIGPPLAFMLALNYGFTTMFSIAAG-----IFVISLALIAI 190
+ + S SL ++GP L FT +++ + ++ AL +
Sbjct: 332 RQGQLQGSLAALT--SLTSIVGPLL---------FTAIYAASITTWNGWAWIAGAALYLL 380

Query: 191 KLPSVPR 197
LP++ R
Sbjct: 381 CLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07820NEISSPPORIN372e-04 Neisseria sp. porin signature.
		>NEISSPPORIN#Neisseria sp. porin signature.

Length = 348

Score = 36.5 bits (84), Expect = 2e-04
Identities = 17/81 (20%), Positives = 30/81 (37%), Gaps = 2/81 (2%)

Query: 367 YHAGEHYQ-GNWFPAYGLLPRWHHASNHACEKPAGLETVTLTYYRDHVEHRVIGGIMRDL 425
YH G +YQ +F Y L + + E ++ + HR++GG +
Sbjct: 180 YHVGLNYQNSGFFAQYAGLFQRYGEGTKKIEYDDQTYSIPSLFVEKLQVHRLVGGYDNNA 239

Query: 426 LTAHQVKLEIQELEYDAWHRG 446
L V + Q+ + G
Sbjct: 240 LYV-SVAAQQQDAKLYGAMSG 259


118KPHS_07120KPHS_07040N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_07120-2100.039971bifunctional aspartokinase I/homoserine
KPHS_07110-1150.005442hypothetical protein
KPHS_07100-115-0.000910putative RNA methyltransferase
KPHS_07090-114-0.277280two-component response regulator
KPHS_070801160.971633hypothetical protein
KPHS_070701141.544177catabolite repression sensor kinase
KPHS_070601130.890613DNA-binding response regulator CreB
KPHS_070501121.186736hypothetical protein
KPHS_070400121.182548right origin-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07120CARBMTKINASE310.025 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 30.6 bits (69), Expect = 0.025
Identities = 22/90 (24%), Positives = 35/90 (38%), Gaps = 11/90 (12%)

Query: 208 DYSAAVLAACLRADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEA---MELSYFGAKV 264
D + LA + AD I TDV+G + L+ + +E E +F A
Sbjct: 216 DLAGEKLAEEVNADIFMILTDVNGAAL-YYGT-EKEQWLREVKVEELRKYYEEGHFKAGS 273

Query: 265 LHPRTIAPIAQFQIPCLIKNTGNPQAPGTL 294
+ P+ +A I +F I+ G L
Sbjct: 274 MGPKVLAAI-RF-----IEWGGERAIIAHL 297


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07090HTHFIS832e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 83.0 bits (205), Expect = 2e-20
Identities = 31/122 (25%), Positives = 60/122 (49%), Gaps = 1/122 (0%)

Query: 1 MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSENDINLVIMDINLPGK 60
M IL+ +D+ R L GYDV ++ A + + ++ D +LV+ D+ +P +
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 NGLLLARELRE-QADVALMFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLS 119
N L +++ + D+ ++ ++ ++ + I E GA DY+ KPF+ EL L+
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 120 RT 121

Sbjct: 121 EP 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07070PF06580389e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 37.5 bits (87), Expect = 9e-05
Identities = 28/94 (29%), Positives = 41/94 (43%), Gaps = 20/94 (21%)

Query: 379 AIDFTPQGGEIALAAEKRNEEVQLSVIDNGCGIPDYALERIFERFYSLPREDGHKSSGLG 438
I PQGG+I L K N V L V + G SL ++ +S+G G
Sbjct: 271 GIAQLPQGGKILLKGTKDNGTVTLEVENTG----------------SLALKNTKESTGTG 314

Query: 439 LAFVREVARLHHGD---INLHNRPEGGVVATLRL 469
L VRE ++ +G I L + +G V A + +
Sbjct: 315 LQNVRERLQMLYGTEAQIKLSEK-QGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07060HTHFIS994e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 98.8 bits (246), Expect = 4e-26
Identities = 36/146 (24%), Positives = 64/146 (43%)

Query: 1 MQQPRIWLVEDEQSIADTLVYMLQQEGFQVSVFGRGLPALEAAAHQAPDVAILDVGLPDI 60
M I + +D+ +I L L + G+ V + A D+ + DV +PD
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 SGFELCRRLLTRYPALPVLFLTARSDEVDKLLGLEIGADDYIAKPFSPREVCARVRTVLR 120
+ F+L R+ P LPVL ++A++ + + E GA DY+ KPF E+ + L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 RLQKFAAPSPVVRVGEFVLDEQAAAI 146
++ + L ++AA+
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAM 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_07040DPTHRIATOXIN290.020 Diphtheria toxin signature.
		>DPTHRIATOXIN#Diphtheria toxin signature.

Length = 567

Score = 29.3 bits (65), Expect = 0.020
Identities = 20/97 (20%), Positives = 44/97 (45%), Gaps = 3/97 (3%)

Query: 139 LGVTQSYTCKLEEISDFRNQMRVQFWRDFLGNSPS-IPPVLYGLHEPRPSLEK--DDEQE 195
+G S +++ D ++ + + G P + + G+ +P+ + DD+ +
Sbjct: 24 IGAPPSAHAGADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWK 83

Query: 196 VFYTTALTPEMANGHLQHAHPVTLEGGEYVMFTYEGL 232
FY+T + A + + +P++ + G V TY GL
Sbjct: 84 GFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGL 120


119KPHS_06550KPHS_06490N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_06550-217-2.710332sigma-54 dependent transcriptional regulator
KPHS_06540-117-2.587445mannitol dehydrogenase domain-containing
KPHS_06530-216-2.114598alcohol dehydrogenase GroES domain-containing
KPHS_06520-116-0.998788GntR family transcriptional regulator
KPHS_065100160.799092major facilitator family transporter
KPHS_065001161.763033putative N-acetyltransferase
KPHS_064901160.967224putative N-acetyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06550HTHFIS1591e-43 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 159 bits (403), Expect = 1e-43
Identities = 79/301 (26%), Positives = 132/301 (43%), Gaps = 27/301 (8%)

Query: 86 MAELLAESDRQPEQADHFSLLTGHDGSLRKPIEQMKTALFYPNGGLPLLITGDSGTGKSY 145
+AE + + + L G ++++ + + L L+ITG+SGTGK
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLM---QTDLTLMITGESGTGKEL 175

Query: 146 MAELMHEFAIAQGLLAPDAPFVSFNCAQYASNPELLAANLFGYVKGAFTGAQSDKAGAFE 205
+A +H++ + + PFV+ N A A +L+ + LFG+ KGAFTGAQ+ G FE
Sbjct: 176 VARALHDYGKRR-----NGPFVAINMA--AIPRDLIESELFGHEKGAFTGAQTRSTGRFE 228

Query: 206 AANGGMLFLDEVHRLDAQGQEKLFTWLDRKEIYRVGETAQGLPISLRLVFATTEDIHS-- 263
A GG LFLDE+ + Q +L L + E VG + +R+V AT +D+
Sbjct: 229 QAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGR-TPIRSDVRIVAATNKDLKQSI 287

Query: 264 ---TFLTTFLRRIPIL-VSLPDLQHRSREEKEALTLQFFWQEARTLAAR-LQLTPRLLQV 318
F R+ ++ + LP L R R E ++ F Q+A + L++
Sbjct: 288 NQGLFREDLYYRLNVVPLRLPPL--RDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALEL 345

Query: 319 LTQYVYRGNVGELKNVVKYAVASAWARSPGREMLTVTLHDLPENVMAATPALSEAMGQQE 378
+ + + GNV EL+N+V+ A P +T + + + P
Sbjct: 346 MKAHPWPGNVRELENLVRRLT----ALYPQD---VITREIIENELRSEIPDSPIEKAAAR 398

Query: 379 P 379

Sbjct: 399 S 399


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06510TCRTETB492e-08 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 48.7 bits (116), Expect = 2e-08
Identities = 71/402 (17%), Positives = 147/402 (36%), Gaps = 39/402 (9%)

Query: 44 MILLFLAAVINYLDRSSLSVANLTIRQELGLNATEIGALLSVFSLAYGIAQLPCGPLLDR 103
+I L + + + L+ L+V+ I + + + F L + I G L D+
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 104 KGPRIMLGLGMFFWSLFQALSGMVHSFTQF-VLVRIGMGIGEAPMNPCGVKVINDWFNIK 162
G + +L G+ + + HSF ++ R G G A + V+ + +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 163 ERGRPMGFFNAASTIGVAVSPPILAAMMLMMGWRWMFIT--------------------- 201
RG+ G + +G V P I + + W ++ +
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRI 195

Query: 202 ------IGILGIFIAIGWYMLYRNREDLPLTADEQAYLNAGSVNVRRDPLSFAEWRSLFK 255
GI+ + + I ++ML+ + ++R+ F + L K
Sbjct: 196 KGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPG-LGK 254

Query: 256 NKT-MWGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGFMAAIPFLFGAAGMLING 314
N M G++ G I T +++ +P ++ + L G + P G ++I G
Sbjct: 255 NIPFMIGVLCGGI-IFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFP---GTMSVIIFG 310

Query: 315 YVTDWLV-KGGMAPIKSRKICIIAGMFCSAAFTLIVPHATTSFAAVLLIGMALFCIHFAG 373
Y+ LV + G + + + ++ F +A+F L + V ++G F
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVIS 370

Query: 374 TSCWGLIHVAVASRMTASVGSIQNFASFICASFAPVVTGFIV 415
T + ++ + + S+ NF SF+ + G ++
Sbjct: 371 TI----VSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06500SACTRNSFRASE364e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.5 bits (84), Expect = 4e-05
Identities = 12/60 (20%), Positives = 23/60 (38%), Gaps = 4/60 (6%)

Query: 80 ALRPAWRGKGLGRKLMQELLMLLQQQGIETVFLEVIRDNHAAVALYQSLGFTRRYGLCGY 139
A+ +R KG+G L+ + + ++ + LE N +A Y F +
Sbjct: 96 AVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI----IGAV 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_06490SACTRNSFRASE341e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 34.1 bits (78), Expect = 1e-04
Identities = 21/93 (22%), Positives = 44/93 (47%), Gaps = 10/93 (10%)

Query: 74 VGFI-LTEPLDDALFIVEVAVHQAWQQQGIGRMLLERVIESARQMGYPAVTLTTFREVPW 132
+G I + + I ++AV + ++++G+G LL + IE A++ + + L T +++
Sbjct: 77 IGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLET-QDI-- 133

Query: 133 NAP---FYTRLGFAM--LDELTLPAGLAAKREQ 160
N FY + F + +D L + E
Sbjct: 134 NISACHFYAKHHFIIGAVD-TMLYSNFPTANEI 165


120KPHS_05860KPHS_05790N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_058600212.494655transcriptional activator protein Irlr
KPHS_05850-1202.666366hypothetical protein
KPHS_05840-1213.023507copper/silver efflux system outer membrane
KPHS_058300233.542969copper-binding protein
KPHS_058200233.930667RND family efflux transporter MFP subunit
KPHS_058101234.551387hypothetical protein
KPHS_058001256.518318cyanate transport
KPHS_057902266.511088lipoprotein Blc
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05860HTHFIS831e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 83.0 bits (205), Expect = 1e-20
Identities = 35/117 (29%), Positives = 61/117 (52%)

Query: 2 KILIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTSDYDLLILDIMLPDVNGWD 61
IL+ +D+ L + L+ AG+ V + N + D DL++ D+++PD N +D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 IVRMLRAAGKGMPILLLTALGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRR 118
++ ++ A +P+L+++A T +K E GA DYL KPF EL+ + L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05840GPOSANCHOR310.009 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 31.2 bits (70), Expect = 0.009
Identities = 29/166 (17%), Positives = 58/166 (34%), Gaps = 9/166 (5%)

Query: 140 RLKNLSEADRQNFFASEEARRAVHILLIANVSQSYFNQRLAAAQLQVANDTLQNYQQSYA 199
A + A + A A L + + +A+++ + A
Sbjct: 204 NFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQA 263

Query: 200 FVEKQLLTGSTTVLALEQARSMIESTRADIAKRQGQLAQANNALQLLLGSYQHLPDDSAS 259
+EK L + + I++ A+ A + + A + Q+L + Q L D +
Sbjct: 264 ELEKAL---EGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDA 320

Query: 260 SAVDLQGVTLPPSLSSAILLQRPDILEAEHSLQAANANIGAARAAF 305
S + + L ++ I EA S Q+ ++ A+R A
Sbjct: 321 SREAKKQL----EAEHQKLEEQNKISEA--SRQSLRRDLDASREAK 360


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05810ACRIFLAVINRP6880.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 688 bits (1777), Expect = 0.0
Identities = 224/1059 (21%), Positives = 437/1059 (41%), Gaps = 54/1059 (5%)

Query: 1 MIEWIIRRSVANRFLVMMAALFLSIWGTWTIIHTPVDALPDLSDVQVIVKTRYPGQAPQI 60
M + IRR + A+ L + G I+ PV P ++ V V YPG Q
Sbjct: 1 MANFFIRR----PIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQT 56

Query: 61 VENQVTWPLTTTMLSVPGARTVRGFSQ-FGDSYVYVIFEDGTDPYWARSRVLEYLNQVQG 119
V++ VT + M + + S G + + F+ GTDP A+ +V L
Sbjct: 57 VQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATP 116

Query: 120 KLPAGVSAEMGP-DATGVGWVFEYALVDRSGKHDLAELRSLQDWFLKYELKTIPNVSEVA 178
LP V + + + ++ V + ++ +K L + V +V
Sbjct: 117 LLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQ 176

Query: 179 SVGGVVKEYQIVVDPMKLTQYGISLGEVKSALDASNQEAGGSSVELA------EAEYMVR 232
G +I +D L +Y ++ +V + L N + + + +
Sbjct: 177 LFGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASII 235

Query: 233 ASGYLQTLDDFKNIVLKTGDNGVPVYLGDVARVQIGPEMRRGIAELNGEGEVAGGVVILR 292
A + ++F + L+ +G V L DVARV++G E IA +NG+ AG + L
Sbjct: 236 AQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGK-PAAGLGIKLA 294

Query: 293 SGKNAREVISAVKEKLASLQSSLPEGVEVVTTYDRSQLIDRAIDNLSYKLLEEFIVVALV 352
+G NA + A+K KLA LQ P+G++V+ YD + + +I + L E ++V LV
Sbjct: 295 TGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLV 354

Query: 353 CALFLWHVRSALVAIISLPLGLCFAFIMMHFQGLNANIMSLGGIAIAVGAMVDAAIVMIE 412
LFL ++R+ L+ I++P+ L F ++ G + N +++ G+ +A+G +VD AIV++E
Sbjct: 355 MYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVE 414

Query: 413 NAHKRLEEWEHQHPGEKLSNDTRWKIITEASVEVGPALFISLLIITLSFIPIFTLEGQEG 472
N + + E + P + ++ ++ AL ++++ FIP+ G G
Sbjct: 415 NVERVMME-DKLPP---------KEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTG 464

Query: 473 KLFGPLAFTKTWSMAGAALLAIVAIPILMGFWIRGRIPAENSNPLNRF----------LI 522
++ + T +MA + L+A++ P L ++ AE+ F +
Sbjct: 465 AIYRQFSITIVSAMALSVLVALILTPALCATLLKPV-SAEHHENKGGFFGWFNTTFDHSV 523

Query: 523 RIYHPLLLKVLHWPKTTLLIALLSILTVVWPLNRVGGEFLPQINEGDLLYMPSTLPGISA 582
Y + K+L LLI L + +V R+ FLP+ ++G L M G +
Sbjct: 524 NHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQ 583

Query: 583 AQAADMLQKTDKLIMA--VPEVARVFGKTGKAETATDSAPLEMVETTIQLKPQDQW-RPG 639
+ +L + + V VF G + + + LKP ++
Sbjct: 584 ERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQN---AGMAFVSLKPWEERNGDE 640

Query: 640 MTMEKIVDELDKTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTNLADIDAIAGQ 699
+ E ++ + + + +++ + I +G + Q
Sbjct: 641 NSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQ 700

Query: 700 IEGVARSVPG-VTSALAERLVGGRYLDIDIQREKAARYGMTVGDVQLFVSSAIGGAMVGE 758
+ G+A P + S L +++ +EKA G+++ D+ +S+A+GG V +
Sbjct: 701 LLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVND 760

Query: 759 TVEGVERYPINIRYPQSYRDSPETLRQLPILTPLKQQIVLGDVAEVKVVTGPSMLKTENA 818
++ + ++ +R PE + +L + + + + V G L+ N
Sbjct: 761 FIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNG 820

Query: 819 RPTSWIYIDARDRDMVSVVHDLQQAIGKEVKLKPGISVSYSGQFELLERANQKLKLMVPM 878
P+ I +A L + + KL GI ++G + + +V +
Sbjct: 821 LPSMEIQGEAAPGTSSGDAMALMENL--ASKLPAGIGYDWTGMSYQERLSGNQAPALVAI 878

Query: 879 TLMIIFVLLYLAFRRVGEALLIITSVPFALVGGIWFLYWMGFHLSVATGTGFIALAGVAA 938
+ +++F+ L + + ++ VP +VG + V G + G++A
Sbjct: 879 SFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSA 938

Query: 939 EFGVVMLMYLRHAIEAEPSLENPQTFSVDKLDEALYQGAVLRVRPKAMTVAVIIAGLLPI 998
+ ++++ + + +E E + EA +R+RP MT I G+LP+
Sbjct: 939 KNAILIVEFAKDLMEKEGK----------GVVEATLMAVRMRLRPILMTSLAFILGVLPL 988

Query: 999 LWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKL 1037
GAGS + + ++GGM++A LL++F +P + +
Sbjct: 989 AISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVV 1027


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_05790BCTLIPOCALIN2331e-81 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 233 bits (595), Expect = 1e-81
Identities = 85/151 (56%), Positives = 111/151 (73%), Gaps = 1/151 (0%)

Query: 25 PKGVQPISGFDASRYLGKWYEVARLENRFERGLEQVTATYGARSDGGISVVNRGYDPVKK 84
P+ V+P+S F+ + YLGKWYEVARL++ FERGL QVTA Y R+DGGISV+NRGY K
Sbjct: 20 PESVKPVSDFELNNYLGKWYEVARLDHSFERGLSQVTAEYRVRNDGGISVLNRGYSEEKG 79

Query: 85 RWNESDGKAYFTGAPTTAALKVSFFGPFYGGYNVIRLD-DDYQYALVSGPNRDYLWILSR 143
W E++GKAYF T LKVSFFGPFYG Y V LD ++Y YA VSGPN +YLW+LSR
Sbjct: 80 EWKEAEGKAYFVNGSTDGYLKVSFFGPFYGSYVVFELDRENYSYAFVSGPNTEYLWLLSR 139

Query: 144 TPTIPAAVKQDYLNTARELGFDVDRLVWIRQ 174
TPT+ + ++ ++E GFD +RL++++Q
Sbjct: 140 TPTVERGILDKFIEMSKERGFDTNRLIYVQQ 170


121KPHS_03370KPHS_03250N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_033703195.334975phosphonate transport ATP-binding protein
KPHS_033604195.345054phosphonate metabolism protein PhnM
KPHS_033503205.133578phosphonate transport ATP-binding protein
KPHS_033402204.826940carbon-phosphorus lyase complex accessory
KPHS_033302195.065818hypothetical protein
KPHS_033200143.550801putative histidine protein kinase
KPHS_03310-2150.517608putative ABC transporter
KPHS_03300-215-0.201824ribose ABC superfamily ATP binding cassette
KPHS_03290-217-1.306090ribose ABC transporter ATP-binding protein
KPHS_03280-119-2.205524putative carbohydrate kinase
KPHS_03270-117-2.898314hypothetical protein
KPHS_03260-117-1.092436hypothetical protein
KPHS_03250016-0.214368putative response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03370PF05272280.027 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.027
Identities = 14/42 (33%), Positives = 19/42 (45%)

Query: 36 CVVLHGHSGSGKSTLLRSLYANYLPDSGHIHIRHGDEWVDLV 77
VVL G G GKSTL+ +L H I G + + +
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQI 639


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03330RTXTOXIND300.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.8 bits (67), Expect = 0.002
Identities = 24/115 (20%), Positives = 47/115 (40%), Gaps = 12/115 (10%)

Query: 13 LSLTSLAARADIIDDAIGNIQQAINDAYNPGSSRSDDDDRYDDDGRYDDGRYQGS----- 67
L LT+L A AD + +Q + SRS + ++ + D+ +Q
Sbjct: 125 LKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEV 184

Query: 68 -------RQQSRDSQRQYDERQRQLDERRRQLDERQRQLDRDRRQLESDQRRLDD 115
++Q Q Q +++ LD++R + +++R ++ RLDD
Sbjct: 185 LRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDD 239


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03320HTHFIS564e-10 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 55.6 bits (134), Expect = 4e-10
Identities = 28/121 (23%), Positives = 58/121 (47%), Gaps = 8/121 (6%)

Query: 646 LVLEDEEDVRQTLCEQLHQLGWLTLETASGEEALQLLEASPDIALLISDLMLPGALSGAD 705
LV +D+ +R L + L + G+ T++ + + A L+++D+++P + D
Sbjct: 7 LVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD-GDLVVTDVVMPDE-NAFD 64

Query: 706 VIHTARRRFPALPVLLISGQDLRPAQNPALPE--VEWLRKPF----TRAQLAQALSAAYA 759
++ ++ P LPVL++S Q+ A + ++L KPF + +AL+
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 760 R 760
R
Sbjct: 125 R 125


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03300PF00577290.043 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 28.7 bits (64), Expect = 0.043
Identities = 10/40 (25%), Positives = 19/40 (47%), Gaps = 2/40 (5%)

Query: 227 FVYGMSGLLSGLGGVMSASRLYSANGNLGVGYELDAIAAV 266
++G+ + GG A R + N G+G + A+ A+
Sbjct: 400 LLHGLPAGWTIYGGTQLADRYRAF--NFGIGKNMGALGAL 437


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03290SUBTILISIN290.019 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 29.4 bits (66), Expect = 0.019
Identities = 16/65 (24%), Positives = 25/65 (38%), Gaps = 5/65 (7%)

Query: 55 KLAGDNVKVTLVSSGYDLGQQVAQIDNFIAAKVDMIIL---NAADSKGIGPAVKRAKEAG 111
L +KV + I I KVD+I + D + AVK+A +
Sbjct: 111 DLLI--IKVLNKQGSGQYDWIIQGIYYAIEQKVDIISMSLGGPEDVPELHEAVKKAVASQ 168

Query: 112 IVVVA 116
I+V+
Sbjct: 169 ILVMC 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_03250HTHFIS909e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.5 bits (222), Expect = 9e-23
Identities = 33/132 (25%), Positives = 62/132 (46%), Gaps = 1/132 (0%)

Query: 2 KPVILVVDDDRAMGELLSDVLGAHAFEVLVSQTGNDALTTVAQRADIALVLLDMILPDTH 61
ILV DDD A+ +L+ L ++V ++ +A D LV+ D+++PD +
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIA-AGDGDLVVTDVVMPDEN 61

Query: 62 GLQVLQQLQRTRPELPVVMLSGLGSESDVVVGLEMGADDYIAKPFSSRVVVARVKAVLRR 121
+L ++++ RP+LPV+++S + + E GA DY+ KPF ++ + L
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 122 SGALAGEASGAG 133
+
Sbjct: 122 PKRRPSKLEDDS 133


122KPHS_02490KPHS_02410N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
KPHS_02490-114-0.285354hypothetical protein
KPHS_02480016-0.620550UDP-3-O-(3-hydroxymyristoyl)-glucosamine
KPHS_02470-115-1.083056glucose-6-phosphate isomerase
KPHS_02460-114-1.432503aspartate kinase III
KPHS_02450-113-2.284552putative cytochrome oxidase
KPHS_02440015-4.433722hypothetical protein
KPHS_02430-114-3.42342323S rRNA pseudouridine synthase F
KPHS_02420116-2.510034SorC family transcriptional regulator
KPHS_02410115-1.493469sorbitol-6-phosphate 2-dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_02490SACTRNSFRASE270.035 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 26.8 bits (59), Expect = 0.035
Identities = 11/43 (25%), Positives = 14/43 (32%)

Query: 102 GHRYGEHIFHAVETRAKTAGESWLWLEVLAANPAARRFYERQG 144
G + H AK L LE N +A FY +
Sbjct: 103 KKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHH 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_02470BCTERIALGSPD320.007 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 31.8 bits (72), Expect = 0.007
Identities = 17/79 (21%), Positives = 34/79 (43%), Gaps = 13/79 (16%)

Query: 69 LAKETDLAGAIKSMFSGEKINR-------TEDRAVLHVALRNRSNTPIVVDGKDVMPEVN 121
AK +DL + + S + + D+ ++ + ++N IV DVM ++
Sbjct: 276 YAKASDLVEVLTGISSTMQSEKQAAKPVAALDKNII-IKAHGQTNALIVTAAPDVMNDLE 334

Query: 122 AVLEKM-----KTFSEAII 135
V+ ++ + EAII
Sbjct: 335 RVIAQLDIRRPQVLVEAII 353


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_0244056KDTSANTIGN250.038 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 25.3 bits (55), Expect = 0.038
Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 5/75 (6%)

Query: 2 ALPRITQKEMTEREQRELKTLLDRARIAHGRPLSNAETNSVKKEYIDKLMAQREAEAKKA 61
LP E + + +EL L+ R + ++NA N + ++ AQ++
Sbjct: 290 GLPNSASIEQIQSKIQELGDTLEELRDSFDGYINNAFVNQIHLNFVMPPQAQQQQG---- 345

Query: 62 RQVRKQQAYKTDKEA 76
Q ++QQA T +EA
Sbjct: 346 -QGQQQQAQATAQEA 359


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_02420HTHFIS280.045 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 28.3 bits (63), Expect = 0.045
Identities = 6/21 (28%), Positives = 12/21 (57%)

Query: 26 QAQIARELGIYRTTISRLLKR 46
Q + A LG+ R T+ + ++
Sbjct: 452 QIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
KPHS_02410DHBDHDRGNASE1154e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 115 bits (289), Expect = 4e-33
Identities = 79/271 (29%), Positives = 126/271 (46%), Gaps = 26/271 (9%)

Query: 7 LKDNVIIVTGGASGIGLAIVDELLSQGAHVQMIDIHGGDRHHNGDNYHF-------WPTD 59
++ + +TG A GIG A+ L SQGAH+ +D + + +P D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 60 ISSATEVQQTIDAIIQRWSRIDGLVNNAGVNFPRLLVDEKAPAGRYELNEAAFEKMVNIN 119
+ + + + I + ID LVN AGV P L+ + L++ +E ++N
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLI---------HSLSDEEWEATFSVN 116

Query: 120 QKGVFFMSQAVARQMVKQRAGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKELG 179
GVF S++V++ M+ +R+G IV V S + YA++KAA FT+ EL
Sbjct: 117 STGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 180 KYGIRVVGVAPGILEKTGLRTPEYEEALAWTRNITVEQLREGYT---KNAIPIGRAGKLS 236
+Y IR V+PG E + W EQ+ +G K IP+ + K S
Sbjct: 177 EYNIRCNIVSPGSTETDMQWS-------LWADENGAEQVIKGSLETFKTGIPLKKLAKPS 229

Query: 237 EVADFVCYLLSARASYITGVTTNIAGGKTRG 267
++AD V +L+S +A +IT + GG T G
Sbjct: 230 DIADAVLFLVSGQAGHITMHNLCVDGGATLG 260



 
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