PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
GenomeAklavik117.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in CP003483 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1HPAKL117_00215HPAKL117_00425Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_00215-116-3.566795hypothetical protein
HPAKL117_00220-218-4.429913adenine specific DNA methyltransferase
HPAKL117_00225-214-2.857983cytosine specific DNA methyltransferase
HPAKL117_00230-111-1.409679hypothetical protein
HPAKL117_00235-111-0.788266type II DNA modification enzyme
HPAKL117_00240112-0.848462type II restriction enzyme
HPAKL117_00245313-0.451234adenine/cytosine DNA methyltransferase
HPAKL117_00250312-0.505052proline permease (putP)
HPAKL117_00255313-1.018655Proline/pyrroline-5-carboxylate dehydrogenase
HPAKL117_00260516-2.119217hypothetical protein
HPAKL117_00265516-2.258050hypothetical protein
HPAKL117_00270314-1.415951hypothetical protein
HPAKL117_00275315-1.078075hypothetical protein
HPAKL117_00280314-0.782024hypothetical protein
HPAKL117_00285217-0.615652hypothetical protein
HPAKL117_002903140.108495hypothetical protein
HPAKL117_003052150.877059hypothetical protein
HPAKL117_003102131.259802hypothetical protein
HPAKL117_003150141.579458ATP-binding protein
HPAKL117_003201132.041378ATP-binding protein
HPAKL117_003255213.629466urease accessory protein UreH
HPAKL117_003305233.500216urease accessory protein
HPAKL117_003353212.680467urease accessory protein UreF
HPAKL117_003402182.516234urease accessory protein UreE
HPAKL117_003453192.553257urea transporter
HPAKL117_003500172.397740urease subunit beta
HPAKL117_00355-2101.500903urease subunit alpha
HPAKL117_003602141.809560*lipoprotein signal peptidase
HPAKL117_003652142.179815phosphoglucosamine mutase
HPAKL117_003702141.54161830S ribosomal protein S20
HPAKL117_003752141.666760peptide chain release factor 1
HPAKL117_003803151.418657reminiscent outer membrane protein HorA
HPAKL117_003853141.267724outer membrane protein HorA
HPAKL117_003900120.737428hypothetical protein
HPAKL117_00395-2110.229383methyl-accepting chemotaxis transducer (tlpC)
HPAKL117_004000130.37624930S ribosomal protein S9
HPAKL117_004051120.59397050S ribosomal protein L13
HPAKL117_004101120.948658hypothetical protein
HPAKL117_004150100.341767malate:quinone oxidoreductase
HPAKL117_004201100.271303hypothetical protein
HPAKL117_004252110.051184RNA polymerase sigma factor RpoD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00255ANTHRAXTOXNA310.037 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 30.9 bits (69), Expect = 0.037
Identities = 36/173 (20%), Positives = 71/173 (41%), Gaps = 19/173 (10%)

Query: 121 QEESQLKERILKRKNEKIILNVNFIGEEVLGEEEANARFEKY---SQALKSNYIQYISIK 177
Q+ S+ ++ + + EK+ F+ E+ + + Y S+ K Y +
Sbjct: 118 QDLSEEEKNSMNSRGEKVPFASRFVFEKKRETPKLIINIKDYAINSEQSKEVYYEIGKGI 177

Query: 178 ITTIFSQINILDFEY-----SKKEIVKRLDALYALALEEEKKQGMPKFINLDMEEFRDLE 232
I S+ LD E+ S + D L++ +E K + K I+++ ++
Sbjct: 178 SLDIISKDKSLDPEFLNLIKSLSDDSDSSDLLFSQKFKE-KLELNNKSIDINF-----IK 231

Query: 233 LTVESFMESIAK-----FDLNAGIVLQAYIPDSYEYLKKLHAFSKERVLKGLK 280
+ F + + F + VL+ Y PD +EY+ KL E++ + LK
Sbjct: 232 ENLTEFQHAFSLAFSYYFAPDHRTVLELYAPDMFEYMNKLEKGGFEKISESLK 284


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00265GPOSANCHOR623e-12 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 61.6 bits (149), Expect = 3e-12
Identities = 54/286 (18%), Positives = 105/286 (36%)

Query: 83 QENSKDNLTKEKDNLTKANTELTEKNKALTTEKDNLTTDLSNAKNQVIQANQEKDKLADK 142
Q ++ + + + D N L KN L+ L ++ A ++ K
Sbjct: 48 QTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKS 107

Query: 143 NKELDAQVGLLKGQIKSLEQSQQALEKENAELTKANADLKTEKDKLTTDLSNAKNQVIQA 202
E +++ L+ + LE++ + + + L+ EK L ++ + + A
Sbjct: 108 LSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA 167

Query: 203 NQEKDKLADKNKELDAQVGLLKGQIKSLEQSQQVLENEKAELTNKITDLSKEKENLTKEK 262
+ K K L+A+ L+ + LE++ + N + KI L EK L K
Sbjct: 168 MNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARK 227

Query: 263 ENLAKEKDNLTTDLSNAKSQVIQANQEKDKLADKNKELDAQVGLLKGQIKSLEQSQQALK 322
+L K + + +++ EK L + EL+ + + + L+
Sbjct: 228 ADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLE 287

Query: 323 NEKDELLREKDNLTTEKDNLTKEKTELTEKNQKLTTEKAELEQKHA 368
EK L EK +L + L + L K +LE +H
Sbjct: 288 AEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQ 333



Score = 59.3 bits (143), Expect = 2e-11
Identities = 54/296 (18%), Positives = 108/296 (36%)

Query: 78 SSNDTQENSKDNLTKEKDNLTKANTELTEKNKALTTEKDNLTTDLSNAKNQVIQANQEKD 137
+N ++ D LT+E N + + + ++ L ++ + + A
Sbjct: 78 FNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFST 137

Query: 138 KLADKNKELDAQVGLLKGQIKSLEQSQQALEKENAELTKANADLKTEKDKLTTDLSNAKN 197
+ K K L+A+ L + LE++ + + + L+ EK L + +
Sbjct: 138 ADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEK 197

Query: 198 QVIQANQEKDKLADKNKELDAQVGLLKGQIKSLEQSQQVLENEKAELTNKITDLSKEKEN 257
+ A + K K L+A+ L + LE++ + N + KI L EK
Sbjct: 198 ALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 257

Query: 258 LTKEKENLAKEKDNLTTDLSNAKSQVIQANQEKDKLADKNKELDAQVGLLKGQIKSLEQS 317
L + L K + + +++ EK L + +L+ Q +L +SL +
Sbjct: 258 LEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRD 317

Query: 318 QQALKNEKDELLREKDNLTTEKDNLTKEKTELTEKNQKLTTEKAELEQKHAPYKKL 373
A + K +L E L + + L K +LE +H ++
Sbjct: 318 LDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQ 373



Score = 32.7 bits (74), Expect = 0.003
Identities = 44/263 (16%), Positives = 102/263 (38%), Gaps = 2/263 (0%)

Query: 8 ENTNTEKNTNISNTTASTPPPNNEESLNNNKELLKSLKELTDRFKKLEVQLEDLEPLIKI 67
+ + T +L K+L+ + +++ LE
Sbjct: 198 ALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 257

Query: 68 SRFIGSFFKKSSNDTQENSKDNLTKEKDNLTKANTELTEKNKALTTEKDNLTTDLSNAKN 127
+ +K+ S + + L L + L + L + + +
Sbjct: 258 LEARQAELEKALEGAMNFST-ADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRR 316

Query: 128 QVIQANQEKDKLADKNKELDAQVGLLKGQIKSLEQSQQALEKENAELTKANADLKTEKDK 187
+ + + K +L ++++L+ Q + + +SL + A + +L + L+ +
Sbjct: 317 DLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKI 376

Query: 188 LTTDLSNAKNQVIQANQEKDKLADKNKELDAQVGLLKGQIKSLEQSQQVLENEKAELTNK 247
+ + + + + K ++ +E ++++ L+ K LE+S+++ E EKAEL K
Sbjct: 377 SEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAK 436

Query: 248 ITDLSK-EKENLTKEKENLAKEK 269
+ +K KE L K+ E LAK +
Sbjct: 437 LEAEAKALKEKLAKQAEELAKLR 459


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00270GPOSANCHOR551e-10 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 55.1 bits (132), Expect = 1e-10
Identities = 49/301 (16%), Positives = 92/301 (30%), Gaps = 3/301 (0%)

Query: 13 QVREELEARISELEDENENLTDENTRLLASKEWLTKENENLAVKISELENSNDQLWQARE 72
+ ++++ + EN L L+ N+ L EL +
Sbjct: 43 VATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLR 102

Query: 73 KLKTENAELLREKDNLAKENTRLLASKEWLTTEKIELIETNQNLAKANTELTKANADLTE 132
K +E + L L + E + L L ADL +
Sbjct: 103 KNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEK 162

Query: 133 KNQKLTTEKIELIETNQKLTKEKTELTEKNKTLTKEKDNLTKANADLKTEKDKINHQVIA 192
+ + L EK L + L K + + + + + A
Sbjct: 163 ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAA 222

Query: 193 LTKEQDSLKQERAQLQDAHGFLEELCANLEKDNQHLTDKLKKLESAQKNLENSNNQLLQA 252
L + L++ + LE + L + +LE A + N +
Sbjct: 223 LAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAK 282

Query: 253 KEKIAEEKIELEREMARLKSLESTDKSELDLQNRRF---KSAIEDLKRQNRKLEEENMAL 309
+ + EK LE E A L+ + R + A + L+ +++KLEE+N
Sbjct: 283 IKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKIS 342

Query: 310 K 310
+
Sbjct: 343 E 343



Score = 42.4 bits (99), Expect = 1e-06
Identities = 53/259 (20%), Positives = 88/259 (33%)

Query: 13 QVREELEARISELEDENENLTDENTRLLASKEWLTKENENLAVKISELENSNDQLWQARE 72
+ E + + + L E L A K L K E + L +
Sbjct: 127 KALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKA 186

Query: 73 KLKTENAELLREKDNLAKENTRLLASKEWLTTEKIELIETNQNLAKANTELTKANADLTE 132
L+ AEL + + +T A + L EK L +L KA + +
Sbjct: 187 ALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSA 246

Query: 133 KNQKLTTEKIELIETNQKLTKEKTELTEKNKTLTKEKDNLTKANADLKTEKDKINHQVIA 192
K + L EK L +L K + + + L A L+ EK + HQ
Sbjct: 247 KIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQV 306

Query: 193 LTKEQDSLKQERAQLQDAHGFLEELCANLEKDNQHLTDKLKKLESAQKNLENSNNQLLQA 252
L + SL+++ ++A LE LE+ N+ + L + QL
Sbjct: 307 LNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAE 366

Query: 253 KEKIAEEKIELEREMARLK 271
+K+ E+ E L+
Sbjct: 367 HQKLEEQNKISEASRQSLR 385


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00350UREASE10450.0 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 1045 bits (2703), Expect = 0.0
Identities = 354/569 (62%), Positives = 443/569 (77%), Gaps = 4/569 (0%)

Query: 3 KISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSN-NP 61
++SR Y +M+GPT GDKVRL DT+L EVE D+T +GEE+KFGGGK +R+GM QS
Sbjct: 4 RMSRAAYANMFGPTVGDKVRLADTELFIEVEKDFTTHGEEVKFGGGKVIRDGMGQSQVTR 63

Query: 62 SKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEAL 121
+D +ITNALI+D+ GI KADIG+KDG+IA IGK GN DMQ GV + VGP TE +
Sbjct: 64 EGGAVDTVITNALILDHWGIVKADIGLKDGRIAAIGKAGNPDMQPGV--TIIVGPGTEVI 121

Query: 122 AGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKW 181
AGEG IVTAGG+D+HIHFI PQQI A SG+T M+GGGTGPA GT ATT TPG ++
Sbjct: 122 AGEGKIVTAGGMDSHIHFICPQQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIAR 181

Query: 182 MLRAAEEYSMNLGFLAKGNASNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKY 241
M+ AA+ + MNL F KGNAS +L + + GA K+HEDWGTTP+AI+ L VAD+Y
Sbjct: 182 MIEAADAFPMNLAFAGKGNASLPGALVEMVLGGATSLKLHEDWGTTPAAIDCCLSVADEY 241

Query: 242 DVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTN 301
DVQV IHTDTLNE+G VEDT+AAI GRT+H +HTEGAGGGHAPDII++ G+ N++P+STN
Sbjct: 242 DVQVMIHTDTLNESGFVEDTIAAIKGRTIHAYHTEGAGGGHAPDIIRICGQPNVIPSSTN 301

Query: 302 PTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSS 361
PT P+TVNT AEH+DMLMVCHHL +I ED+ FA+SRIR +TIAAED LHD+G FSI SS
Sbjct: 302 PTRPYTVNTLAEHLDMLMVCHHLSPTIPEDIAFAESRIRKETIAAEDILHDIGAFSIISS 361

Query: 362 DSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISE 421
DSQAMGRVGEV RTWQTADK K++ GRLKEE GDNDNFR+KRY++KYTINPAIAHG+S
Sbjct: 362 DSQAMGRVGEVAIRTWQTADKMKRQRGRLKEETGDNDNFRVKRYIAKYTINPAIAHGLSH 421

Query: 422 YVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAH 481
+GS+EVGK ADLVLW+PAFFGVKP+M++ GG IA + MGD NASIPTPQPV+YR MF
Sbjct: 422 EIGSLEVGKRADLVLWNPAFFGVKPDMVLLGGTIAAAPMGDPNASIPTPQPVHYRPMFGA 481

Query: 482 HGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCR-NITKKDMQFNDTTAHIEVNP 540
+G+++ ++++TFVSQA+ D G+ LG+ ++++ V+N R I K M N T HIEV+P
Sbjct: 482 YGRSRTNSSVTFVSQASLDAGLAGRLGVAKELVAVQNTRGGIGKASMIHNSLTPHIEVDP 541

Query: 541 ETYHVFVDGKEVTSKPANKVSLAQLFSIF 569
ETY V DG+ +T +PA + +AQ + +F
Sbjct: 542 ETYEVRADGELLTCEPATVLPMAQRYFLF 570


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00385GPOSANCHOR399e-05 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 38.9 bits (90), Expect = 9e-05
Identities = 55/369 (14%), Positives = 126/369 (34%), Gaps = 13/369 (3%)

Query: 48 TNNSKESVLKESDQASQQQQAQKTATNDSTQASTAENTATTDNTTATTDKTYTDTKNNDT 107
N + + E + + + + S ++ + A +K N T
Sbjct: 79 NNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQEL-EARKADLEKALEGAMNFST 137

Query: 108 AVVDAAQQVESDNSAVQTAEKALEKAVDQVKADANATDFDETKFTADQAAEQKAETALKN 167
A + +E++ +A+ + LEKA++ + A A++AA + + L+
Sbjct: 138 ADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEK 197

Query: 168 AEDKLNTDQQTLETDLQQ-----QASQQQQAKTTATNDSTQASTAENTATTDNTTATTDK 222
A + ++ A ++A + + ++A A
Sbjct: 198 ALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 257

Query: 223 TYENSPDTTVA-----GAAKQVVADETTVQQDETALKNAVEKVQQDADVSATDFDETKFT 277
+ A + A T++ ++ AL+ ++ + V + +
Sbjct: 258 LEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLR-- 315

Query: 278 ADQAAEQKAETALKNAEDKLTTDQQTLETDLQQQAQTTATNDSTQAQTLENTAKTDSTTA 337
D A ++A+ L+ KL + E Q + + + Q K +
Sbjct: 316 RDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375

Query: 338 TTDETYTNTKNNDTAVVDAAQQVESDNSAVQTAEKALEKAVDQVKADANATDFDETKFTA 397
++ + + + + A +A +QVE + ALEK +++ T+ ++ + A
Sbjct: 376 ISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQA 435

Query: 398 DQAAEQKAE 406
AE KA
Sbjct: 436 KLEAEAKAL 444


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00425IGASERPTASE330.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 33.5 bits (76), Expect = 0.004
Identities = 30/172 (17%), Positives = 60/172 (34%), Gaps = 14/172 (8%)

Query: 2 KKKANEEKAPKRAKQEAKTEAAQENKA--KENNKAK-ESKTKESKIKEAKTKESKIKETK 58
+K + +E EA KA + N A+ S+TKE++ E K + KE K
Sbjct: 1052 EKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEK 1111

Query: 59 AK--------EPVPVKKLSFNEALEELF---ANSLSDCVSYESIIQISAKVPTLAQVKKI 107
AK P ++S + E A + +I + ++ T A ++
Sbjct: 1112 AKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQP 1171

Query: 108 KELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEKQKVLDEELEDGYDFLKEKD 159
+ ++ V+ S N++ + + + + K +
Sbjct: 1172 AKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRH 1223



Score = 33.5 bits (76), Expect = 0.004
Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 11/71 (15%)

Query: 8 EKAPKRAKQEAKTEAAQENKAKE-----NNKAKESK------TKESKIKEAKTKESKIKE 56
E + +KQE+KT E A E AKE+K T+ +++ ++ ++ + +
Sbjct: 1038 ETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQT 1097

Query: 57 TKAKEPVPVKK 67
T+ KE V+K
Sbjct: 1098 TETKETATVEK 1108


2HPAKL117_00920HPAKL117_01020Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_00920-2113.014533hypothetical protein
HPAKL117_00925-2122.915193hypothetical protein
HPAKL117_00940-2102.359547hypothetical protein
HPAKL117_00945-292.452030hypothetical protein
HPAKL117_00950-1103.331544fumarate reductase iron-sulfur subunit
HPAKL117_00955-1103.353835fumarate reductase flavoprotein subunit
HPAKL117_00960-2131.946936fumarate reductase cytochrome b-556 subunit
HPAKL117_00965-2141.969018triosephosphate isomerase
HPAKL117_00970-2153.218302enoyl-(acyl carrier protein) reductase
HPAKL117_00975-2153.441059UDP-3-O-[3-hydroxymyristoyl] glucosamine
HPAKL117_00980-2153.727527S-adenosylmethionine synthetase
HPAKL117_00985-1183.011637mulitfunctional nucleoside diphosphate
HPAKL117_00990-2192.316484hypothetical protein
HPAKL117_00995-1120.52310250S ribosomal protein L32
HPAKL117_01000-112-0.584766putative phosphate acyltransferase
HPAKL117_01005114-3.2973543-oxoacyl-(acyl carrier protein) synthase III
HPAKL117_01010419-5.633554hypothetical protein
HPAKL117_01015314-3.088144hypothetical protein
HPAKL117_01020112-3.210954hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00925IGASERPTASE300.011 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.0 bits (67), Expect = 0.011
Identities = 31/124 (25%), Positives = 47/124 (37%), Gaps = 10/124 (8%)

Query: 84 SKQESDLDKVADNVKKQESDAFKMPTQTNQTQTEMKTTEETQET-KKELKAVESTGAQKE 142
+ ++ V K+ K TQTN+ T+ETQ T KE VE K
Sbjct: 1055 EQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKV 1114

Query: 143 SQAVAKKEPPHKKPKVMPKD------KANGDKANKDKAKHATKEPKAK--KEAHKEVPKK 194
+ +E P +V PK + + A ++ KEP+++ A E P K
Sbjct: 1115 -ETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAK 1173

Query: 195 ANSK 198
S
Sbjct: 1174 ETSS 1177


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00970DHBDHDRGNASE592e-12 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 59.3 bits (143), Expect = 2e-12
Identities = 61/263 (23%), Positives = 109/263 (41%), Gaps = 29/263 (11%)

Query: 4 LKGKKGLIVGVANNKSIAYGIAQSCFNQGATL-AFTYLNESLEKRVRPIAQELDSPYVYE 62
++GK I G A + I +A++ +QGA + A Y E LEK V + E +
Sbjct: 6 IEGKIAFITGAA--QGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63

Query: 63 LDVSKEEHFKLLYDSVKKDLGSLDFIVHSVAF--------APKEALEGSLLETSKSAFNT 114
DV + +++++G +D +V+ E E + S FN
Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 115 AMEISVYSLIELTNTLKPLLNNGASVLTLSYLGSTKYMAHYNVMGLAKAALESAVRYLAV 174
+ +S Y + + ++ + +N A V S MA Y +KAA + L +
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTS-------MAAY---ASSKAAAVMFTKCLGL 173

Query: 175 DLGKHNIRVNALSAGPIRT-----LASSGIADFRMILKWNE---INAPLRKNVSLEEVGN 226
+L ++NIR N +S G T L + ++I E PL+K ++ +
Sbjct: 174 ELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIAD 233

Query: 227 AGMYLLSSLSSGVSGEVHFVDAG 249
A ++L+S + ++ VD G
Sbjct: 234 AVLFLVSGQAGHITMHNLCVDGG 256


3HPAKL117_01475HPAKL117_01620Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_014751143.43877650S ribosomal protein L21
HPAKL117_014801143.38688150S ribosomal protein L27
HPAKL117_014851133.551778periplasmic dipeptide-binding protein
HPAKL117_014901143.926079dipeptide permease
HPAKL117_01495-1123.267248dipeptide permease
HPAKL117_01500-2133.130313dipeptide ABC transporter
HPAKL117_01505-2142.945999dipeptide ABC transporter
HPAKL117_01510-1132.515842GTPase CgtA
HPAKL117_01515-1131.775920hypothetical protein
HPAKL117_015200162.476128hypothetical protein
HPAKL117_015251172.913886glutamate-1-semialdehyde aminotransferase
HPAKL117_015304162.107863hypothetical protein
HPAKL117_015354141.813201hypothetical protein
HPAKL117_015404132.159081hypothetical protein
HPAKL117_015451111.130760hypothetical protein
HPAKL117_015501120.214846hypothetical protein
HPAKL117_01555013-0.118883ATP-binding protein
HPAKL117_01560115-0.643159nitrite extrusion protein NarK
HPAKL117_01565216-1.250512putative heme iron utilization protein
HPAKL117_01570315-1.756634arginyl-tRNA synthetase
HPAKL117_01575216-1.384827Sec-independent protein translocase protein
HPAKL117_01580214-0.998270guanylate kinase
HPAKL117_01585012-1.172334poly E-rich protein
HPAKL117_01590-111-1.892213hypothetical protein
HPAKL117_01595-112-2.123228nuclease NucT
HPAKL117_01600112-1.915388outer membrane protein HorC
HPAKL117_01605213-2.111018flagellar basal body L-ring protein
HPAKL117_01610314-1.780206CMP-N-acetylneuraminic acid synthetase
HPAKL117_01615211-1.016493CMP-N-acetyl neuraminic acid synthetase
HPAKL117_01620211-0.473317flagellar biosynthesis protein G
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01560TCRTETA453e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 45.2 bits (107), Expect = 3e-07
Identities = 54/271 (19%), Positives = 102/271 (37%), Gaps = 16/271 (5%)

Query: 28 LILSGSLTPHQSFQLGIAVLMGYVFGSFLIQFLSPLMSLESIAKISFGLIALSFLICYFD 87
L+ S +T H L + LM + L LS + +S A+ + I
Sbjct: 35 LVHSNDVTAHYGILLALYALMQFACAPVLGA-LSDRFGRRPVLLVSLAGAAVDYAI--MA 91

Query: 88 SIPFFW-LWIWRFIAGVASSALMILVAPLSLPYVKENKRALVGGFIFSAVGIGSVFSGFV 146
+ PF W L+I R +AG+ + A + ++RA GF+ + G G V +
Sbjct: 92 TAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVL 150

Query: 147 LPWISSYNIKWAWIFLGGSCLIAFILSLIGLKN-HSLRKKSIKKEESAFKIPFHL----- 200
+ ++ + + F+ L H ++ +++E F
Sbjct: 151 GGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMT 210

Query: 201 ---WLLLISCALNAIGFLPHTLFWVDYLIRHLNISPAIAGTSWALFG-FGATLGSLISGP 256
L+ + + +G +P L WV + + G S A FG + ++I+GP
Sbjct: 211 VVAALMAVFFIMQLVGQVPAAL-WVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGP 269

Query: 257 MAQKLGAKNANIFILVLKSIACFLPIFFHQI 287
+A +LG + A + ++ L F +
Sbjct: 270 VAARLGERRALMLGMIADGTGYILLAFATRG 300


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01580PF05272290.011 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.011
Identities = 9/18 (50%), Positives = 11/18 (61%)

Query: 8 LILSGPSGAGKSTLTKYL 25
++L G G GKSTL L
Sbjct: 599 VVLEGTGGIGKSTLINTL 616


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01585IGASERPTASE653e-13 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 65.1 bits (158), Expect = 3e-13
Identities = 49/268 (18%), Positives = 91/268 (33%), Gaps = 25/268 (9%)

Query: 173 EIKEEVKETPQEEEKPKEDEIQESETQKSEEV--PKELETQEELEIPKEETQEQAKEQEP 230
E + + +T S +EE+ E ET E E
Sbjct: 986 EKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSK 1045

Query: 231 IKEETQE-------EVKEEMQEVKEEKQEKTQDSPSVQELEAMQELVKEIQENSNSQEDK 283
+ +T E E + +EV +E + + + E+ KE Q +
Sbjct: 1046 QESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETAT 1105

Query: 284 KETQESAETPQETEKQELETPQENAETPQEKTQENTETPQENPETPQEETQEKKAQEDHY 343
E +E A+ ETEK + E P+ ++ ++ Q T PQ P + T
Sbjct: 1106 VEKEEKAKV--ETEKTQ-EVPKVTSQVSPKQEQSETVQPQAEPARENDPT---------- 1152

Query: 344 ESIEDIPEPVMAQAMGEALPFLNESVAKTPNNENDTKTPKESVIKTPQEKEGSDKASSPL 403
+I++ A E S + P E+ T SV++ P+ +
Sbjct: 1153 VNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQP--- 1209

Query: 404 ELRLNLQDLLKSLNQESLKSLLENKTLS 431
+ + K+ ++ S++S+ N +
Sbjct: 1210 TVNSESSNKPKNRHRRSVRSVPHNVEPA 1237



Score = 63.5 bits (154), Expect = 1e-12
Identities = 49/214 (22%), Positives = 74/214 (34%), Gaps = 25/214 (11%)

Query: 162 LPTLNDQEVKEEIKEEVKETPQEEEKPKEDEIQESETQKSE--EVPKELETQE--ELEIP 217
P+ + V E K+E K + E+ E Q E K V +T E +
Sbjct: 1032 TPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE 1091

Query: 218 KEETQEQAKEQEPI--KEETQEEVKEEMQEV---------KEEKQEKTQDSPSVQELEAM 266
+ETQ ++ KEE + E+ QEV K+E+ E Q
Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP 1151

Query: 267 QELVKEIQENSNSQEDK---KETQESAETPQETEKQELETPQENAETPQEKTQENTETPQ 323
+KE Q +N+ D + S TE + T E P+ T T+ P
Sbjct: 1152 TVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQ-PT 1210

Query: 324 ENPETPQEETQEKKAQEDHYESIEDIPEPVMAQA 357
N E+ K + H S+ +P V
Sbjct: 1211 VNSES------SNKPKNRHRRSVRSVPHNVEPAT 1238



Score = 46.2 bits (109), Expect = 2e-07
Identities = 27/175 (15%), Positives = 55/175 (31%), Gaps = 10/175 (5%)

Query: 152 QEEPNNEEQLLPTLNDQEVKEEIKEEVKETPQE-----EEKPKEDEIQESETQKSEEVPK 206
E + E +E+ K E ++T + + PK++ Q Q E +
Sbjct: 1090 SETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQE--QSETVQPQAEPAR 1147

Query: 207 ELETQEELEIPKEETQEQAKEQEPIKEETQEEVKEEMQEVKEEKQEKTQDSPSVQELEAM 266
E + ++ P+ +T A ++P KE + + + ++P
Sbjct: 1148 ENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATT 1207

Query: 267 QELVKEIQENSNSQEDKKETQESAETPQETEKQELETPQENAETPQEKTQENTET 321
Q V N ++ + P E + + + T NT
Sbjct: 1208 QPTVNSESSNKPKNRHRRSVR---SVPHNVEPATTSSNDRSTVALCDLTSTNTNA 1259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01605FLGLRINGFLGH1912e-63 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 191 bits (487), Expect = 2e-63
Identities = 51/172 (29%), Positives = 84/172 (48%), Gaps = 18/172 (10%)

Query: 56 GERPLFADRRAMKPNDLITIIVSEKASANYSSS----KDYKSASGGNSTPPRLTYNGLDE 111
G +PLF DRR D +TI++ E SA+ SSS +D K+ G ++ P L GL
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYL--QGLFG 118

Query: 112 RKKQEAQYLDDKNNYNFTKSSNNTNFKGGGSQKKSEDLEIVLSARIIKVLENGNYFIYGN 171
+ + + S F G G S L+ + +VL NGN + G
Sbjct: 119 NARADVEA------------SGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGE 166

Query: 172 KEVLVDGEKQILKVSGVIRPYDIERNNTIQSKFLADAKIEYTNLGHLSDSNK 223
K++ ++ + ++ SGV+ P I +NT+ S +ADA+IEY G+++++
Sbjct: 167 KQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN 218


4HPAKL117_02135HPAKL117_02325Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_02135-116-3.304433polyphosphate kinase
HPAKL117_02140319-4.965952*hypothetical protein
HPAKL117_02145319-4.972620type I restriction enzyme specificity subunit
HPAKL117_02150418-5.343204type I restriction enzyme M protein
HPAKL117_02155721-6.469951type I restriction enzyme R protein
HPAKL117_02160822-6.760993hypothetical protein
HPAKL117_02165725-7.135441integrase/recombinase (xerD)
HPAKL117_02170625-6.880481hypothetical protein
HPAKL117_02175925-6.296969hypothetical protein
HPAKL117_02180927-5.883911hypothetical protein
HPAKL117_021851027-5.535339hypothetical protein
HPAKL117_021901027-5.228078hypothetical protein
HPAKL117_021951125-5.466848hypothetical protein
HPAKL117_022001226-5.204440hypothetical protein
HPAKL117_022051028-6.299730hypothetical protein
HPAKL117_022101229-6.892280hypothetical protein
HPAKL117_02215718-4.073219hypothetical protein
HPAKL117_02220618-4.162339hypothetical protein
HPAKL117_02225618-4.234531hypothetical protein
HPAKL117_02230718-4.185898topoisomerase I
HPAKL117_02235617-3.901635hypothetical protein
HPAKL117_02240617-3.963236hypothetical protein
HPAKL117_02245728-4.497967VirD4 coupling protein
HPAKL117_02250727-4.010306hypothetical protein
HPAKL117_02255827-4.040154VirB11 type IV secretion ATPase
HPAKL117_02260727-4.483599hypothetical protein
HPAKL117_02265625-5.161651hypothetical protein
HPAKL117_02270725-5.099534hypothetical protein
HPAKL117_02275623-6.306222VirB10 type IV secretion protein
HPAKL117_02280623-6.976206type IV secretion system protein VirB9
HPAKL117_02285723-7.426276VirB8 type IV secretion protein
HPAKL117_02290821-7.374608VirB4 type IV secretion ATPase
HPAKL117_02295622-6.042399hypothetical protein
HPAKL117_02300220-4.723996hypothetical protein
HPAKL117_02305114-3.859868hypothetical protein
HPAKL117_02310014-3.720238hypothetical protein
HPAKL117_02315014-3.167804hypothetical protein
HPAKL117_02320115-2.338840type I restriction enzyme R protein
HPAKL117_02325214-2.270259type I restriction enzyme R protein HsdR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02200IGASERPTASE382e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 37.7 bits (87), Expect = 2e-04
Identities = 41/191 (21%), Positives = 69/191 (36%), Gaps = 7/191 (3%)

Query: 477 KEQNKANENAQQETK----NSQAQETTSSQAYESVSQA--NTQATAQANESIKPQTNNTE 530
+ EN++QE+K N Q T++Q E +A N +A Q NE + + E
Sbjct: 1035 ETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKE 1094

Query: 531 TQQENAKESQAIE-QNRATQEPHAKEEPKKVSHPDEPWLDYDPKTHAGLKERQENQEKTP 589
TQ KE+ +E + +A E +E KV+ P + + +EN
Sbjct: 1095 TQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVN 1154

Query: 590 SKGNDEPWIEFAKLEQARAKSHYQAMVEKEKAKQLAQQNAKNTNSINTPNKEVEIIDYGY 649
K A EQ ++ ++ + N+ N NT +
Sbjct: 1155 IKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSE 1214

Query: 650 TQSAPAKNRRR 660
+ + P RR
Sbjct: 1215 SSNKPKNRHRR 1225


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02215adhesinb280.040 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 27.5 bits (61), Expect = 0.040
Identities = 9/45 (20%), Positives = 23/45 (51%), Gaps = 4/45 (8%)

Query: 147 PTLKERKALIDFIKENNPSDKITLLESSLSERIVYKRSVSEGLGV 191
T + K L++ +++ +ESS+ +R + ++VS+ +
Sbjct: 232 GTPDQIKTLVEKLRKTKV--PSLFVESSVDDRPM--KTVSKDTNI 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02245CHANLCOLICIN330.004 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 33.1 bits (75), Expect = 0.004
Identities = 32/205 (15%), Positives = 68/205 (33%), Gaps = 19/205 (9%)

Query: 530 EDAEIVSKEVGEFTRQSKNYSTEKGQLV---------FGGSSSYSHEGRNLLTAQDIMNI 580
++AE K E ++ K EK + ++ S E + + AQ ++
Sbjct: 141 KEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSA 200

Query: 581 ESDEVIVIVTGAKATPLKLKANYWFKDKELLKRANLPIDLEVERRRVEEPTQPTTEITTP 640
EV+ + K +L ++ +D E+ A +L + +E + +++
Sbjct: 201 AQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPR 260

Query: 641 PNQN-------KANLDQSNKGENTENKDNESKTNENNPTIPQKE---SENSNLTESEKDH 690
N +A + G+ E K + +E + + + S +
Sbjct: 261 ANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRN 320

Query: 691 ENPTTSQEKSKNENTEQENDETDEI 715
E +N Q N +I
Sbjct: 321 AGIARVHEAEENLKKAQNNLLNSQI 345


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02270cloacin373e-04 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 36.6 bits (84), Expect = 3e-04
Identities = 30/113 (26%), Positives = 38/113 (33%), Gaps = 30/113 (26%)

Query: 150 IAGSDGSSGANGSNGANGSSGAN---GSNGANGSNGTNSYQGVGSNPFPPIAGSGSGSSG 206
++G DG G N S+ N G G G + G S P GSGSG
Sbjct: 1 MSGGDG----RGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG--- 53

Query: 207 SSNSGYTPFISEGGGIGSMGGGFIPFPYSPGLQNGSGANGSNGTSGANGSNSA 259
I GGG G GG + G +GT G + +A
Sbjct: 54 ---------IHWGGGSGHGNGG-----------GNGNSGGGSGTGGNLSAVAA 86



Score = 36.2 bits (83), Expect = 5e-04
Identities = 20/81 (24%), Positives = 30/81 (37%), Gaps = 3/81 (3%)

Query: 133 NGKGFSGSGTSGTST--NGIAGSDGSSGANGSNGANGSSGANGSNGANGSNGTNSYQGVG 190
G GSG S + G +GS G +G NG N G+ ++
Sbjct: 30 GGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG-NGGGNGNSGGGSGTGGNLSAVAAPV 88

Query: 191 SNPFPPIAGSGSGSSGSSNSG 211
+ FP ++ G+G S S
Sbjct: 89 AFGFPALSTPGAGGLAVSISA 109



Score = 35.1 bits (80), Expect = 0.001
Identities = 23/83 (27%), Positives = 33/83 (39%), Gaps = 6/83 (7%)

Query: 133 NGKGFSGSGT-SGTSTNGIAGSDGSSGANGSNGANGSSGANGSNGANGSNGTNSYQGVGS 191
N S SG +G T G S G+ S+ N G +GS G + G
Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69

Query: 192 NPFPPIAGSGSGSSGSSNSGYTP 214
N +G GSG+ G+ ++ P
Sbjct: 70 N-----SGGGSGTGGNLSAVAAP 87



Score = 33.1 bits (75), Expect = 0.004
Identities = 25/89 (28%), Positives = 33/89 (37%), Gaps = 9/89 (10%)

Query: 140 SGTSGTSTNGIAGSDGSSGANGSNGANGSSGANGSNGANGSNGTNSYQGVGSNPFPPIAG 199
SG G N A S + G G GA+ +G + N G GS G
Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGI-HWGGG 59

Query: 200 SGSGSSGSSNSGYTPFISEGGGIGSMGGG 228
SG G+ G + + GGG G+ G
Sbjct: 60 SGHGNGGGNGNS-------GGGSGTGGNL 81



Score = 31.2 bits (70), Expect = 0.018
Identities = 22/82 (26%), Positives = 32/82 (39%), Gaps = 2/82 (2%)

Query: 130 SSKNGKGFSGSGTSGTSTNGIAGSDGSSGANGSNGANGSSGANGSNGANGSNGTNSYQGV 189
S +G+G + G TS N G G G++ +G S N G +G + G
Sbjct: 2 SGGDGRGHNT-GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60

Query: 190 GSNPFPPIAGSGSGSSGSSNSG 211
G SG G SG+ +
Sbjct: 61 GHGNGGGNGNSG-GGSGTGGNL 81


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02285PF04335891e-22 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 88.7 bits (220), Expect = 1e-22
Identities = 37/217 (17%), Positives = 74/217 (34%), Gaps = 15/217 (6%)

Query: 119 ESFKKDELDLSSVFEIQRKNTQMAYRLAIGGLIGVISLSVALAFLMPLKQIEPYFVDFAN 178
F++ ++ ++A+ +A + VA+A L PLK +EPY +
Sbjct: 12 AYFEEAASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDR 71

Query: 179 SDKHFAVVQ--KADTKINYGEAFSRNLVGSYIMGRETINHIDDKIRLNETIREQSSDEVW 236
+ ++ D I Y EA + + +Y+ RE + + + S+
Sbjct: 72 NTGEASIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYF-DAVMVMSARPEQ 130

Query: 237 KTLEQLVSGKG-----SIYSNSNMDREIKIINISIYKQGKQQNIAVADIMAKVFDKGYLI 291
+ +I +N D ++I +S Q V V
Sbjct: 131 DRWSRFYKTDNPQSPQNILANRT-DVFVEIKRVSFLGGNVAQ---VYFTKESVTGSN--S 184

Query: 292 SEKRYRVSLIYHFKPLIQFDYNSMPKNPTGFMVDKYS 328
++ ++ Y + + KNP G+ V+ Y
Sbjct: 185 TKTDAVATIKYKVDGTPSKEVDRF-KNPLGYQVESYR 220


5HPAKL117_02370HPAKL117_02465Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_02370012-3.465510molybdenum ABC transporter ModA
HPAKL117_02375-110-3.509214molybdenum ABC transporter ModB
HPAKL117_02380-17-1.846677molybdenum ABC transporter ModD
HPAKL117_02385-28-2.113486glutamyl-tRNA synthetase
HPAKL117_02390-29-2.213540outer membrane protein (omp22)
HPAKL117_02395010-0.517264type II adenine specific methyltransferase
HPAKL117_024003130.912338type II adenine specific methyltransferase
HPAKL117_024050130.391254GTP-binding protein TypA
HPAKL117_02410216-0.477664type II adenine specific DNA methyltransferase
HPAKL117_024251150.200567catalase-like protein
HPAKL117_024303160.328391outer membrane protein HofC
HPAKL117_02435315-0.929129hypothetical protein
HPAKL117_02440515-1.896410hypothetical protein
HPAKL117_02445013-0.497007hypothetical protein
HPAKL117_02450112-1.027458putative potassium channel protein
HPAKL117_02455312-1.87266950S ribosomal protein L28
HPAKL117_02460212-2.048030hypothetical protein
HPAKL117_02465211-2.050606neuraminyllactose-binding hemagglutinin
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02405TCRTETOQM1966e-57 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 196 bits (500), Expect = 6e-57
Identities = 115/461 (24%), Positives = 190/461 (41%), Gaps = 67/461 (14%)

Query: 3 NIRNIAVIAHVDHGKTTLVDGLLSQSGTFSEREKVDE--RVMDSNDLERERGITILSKNT 60
I NI V+AHVD GKTTL + LL SG +E VD+ D+ LER+RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 61 AIYYKDTKINIIDTPGHADFGGEVERVLKMVDGVLLLVDAQEGVMPQTKFVVKKALSFGI 120
+ +++TK+NIIDTPGH DF EV R L ++DG +LL+ A++GV QT+ + GI
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 121 CPIVVVNKIDKPAAEPDRVVDEVFDLF---------VAMGASDKQLDFPV-----VYAAA 166
I +NKID+ + V ++ + V + + +F
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 167 RDGYAMKSLDDE----------------------------KKNL--EPLFETILEHVPSP 196
D K + + K N+ + L E I S
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSS 241

Query: 197 SGSVDEPLQMQIFTLDYDNYVGKIGIARVFNGSVKKNESVLLMKSDGSKENGRITKLIGF 256
+ L ++F ++Y ++ R+++G + +SV + KE +IT++
Sbjct: 242 THRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRI----SEKEKIKITEMYTS 297

Query: 257 LGLARTEIENAYAGDIVAIAG--FNAMDV-GDSVVDPINPMPLDPMHLEEPTMSVYFAVN 313
+ +I+ AY+G+IV + V GD+ + P +P P + +
Sbjct: 298 INGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENP----LPLLQTTVEPS 353

Query: 314 DSPLAGLEGKHVTANKLKDRLLKEMQTNIAMKCEEMGEGKFKVSGRGELQITILAENLRR 373
+ + D LL+ + + +S G++Q+ + L+
Sbjct: 354 KPQQREMLLDALLEISDSDPLLRYYVDSAT--------HEIILSFLGKVQMEVTCALLQE 405

Query: 374 E-GFEFSISRPEVIIKEENGVKCEPFEHLVIDTPQDFSGAI 413
+ E I P VI E K E H+ + P F +I
Sbjct: 406 KYHVEIEIKEPTVIYMERPLKKAEYTIHIEVP-PNPFWASI 445



Score = 41.8 bits (98), Expect = 8e-06
Identities = 20/80 (25%), Positives = 30/80 (37%), Gaps = 1/80 (1%)

Query: 396 EPFEHLVIDTPQDFSGAIIERLGKRKAEMKAMNPMSDGYTRLEFEIPARGLIGYRSEFLT 455
EP+ I PQ++ K A + + + L EIPAR + YRS+
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCIQEYRSDLTF 595

Query: 456 DTKGEGVMNHSFLEFRPFSG 475
T G V + +G
Sbjct: 596 FTNGRSVCLTELKGYHVTTG 615


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02465PF052112568e-88 Neuraminyllactose-binding hemagglutinin
		>PF05211#Neuraminyllactose-binding hemagglutinin

Length = 260

Score = 256 bits (655), Expect = 8e-88
Identities = 61/266 (22%), Positives = 115/266 (43%), Gaps = 38/266 (14%)

Query: 18 KTLIALGLSSVLVGCAIKPVAEVKPQNQQEKPIQVNEKIQTTQKVTPLNFNYSLHVAQAA 77
K L+ + ++LVGC+ I+T + LN+ + A
Sbjct: 14 KCLLGASVVALLVGCS-------------------PHIIETNEVALKLNY-HPASEKVQA 53

Query: 78 QNYRLIGILAPRIQVSDNL-KPYIDKFQDALINQIQTIFEKRGYQTLFF--KDESALTLQ 134
+ + I +L P Q SDN+ K Y +KF++ +++ I + +GY+ + D+ +
Sbjct: 54 LDEK-ILLLRPAFQYSDNIAKEYENKFKNQTTLKVEQILQNQGYKVINVDSSDKDDFSFA 112

Query: 135 DKRKLFAVLDVKGWVGILEDLKMNLKDPNNTNALTTQ----------SSGSVWFSFYEPE 184
K++ + + + G + + D K ++ + L + +G V + EP
Sbjct: 113 QKKEGYLAVAMNGEIVLRPDPKRTIQKKSEPGLLFSTGLDKMEGVLIPAGFVKVTILEPM 172

Query: 185 SYRVISDFNVGLG--DFQAIVRAYNNNHSLGFNLKNNLFAGLEKNKQDAIHQILNKIYAL 242
S + F + L D Q + S L + + G + N DAI LNKI+A
Sbjct: 173 SGESLDSFTMDLSELDIQEKFLKTTH-SSHSGGLVSTMVKGTD-NSNDAIKSALNKIFAN 230

Query: 243 IMKKAVTELTEKNISQYKEAIDKMKG 268
IM++ +LT+KN+ Y++ ++KG
Sbjct: 231 IMQEIDKKLTQKNLESYQKDAKELKG 256


6HPAKL117_03145HPAKL117_03170Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_03145-115-3.942520chorismate synthase
HPAKL117_03150-115-5.378032hypothetical protein
HPAKL117_03155-114-4.057184coproporphyrinogen III oxidase
HPAKL117_03160-114-3.542342anaerobic glycerol-3-phosphate dehydrogenase
HPAKL117_03165320-5.372848hypothetical protein
HPAKL117_03170118-4.890349hypothetical protein
7HPAKL117_03220HPAKL117_03295Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_032204140.914776hypothetical protein
HPAKL117_032252100.610921hypothetical protein
HPAKL117_032300100.156931bifunctional N-acetylglucosamine-1-phosphate
HPAKL117_032350100.702531hypothetical protein
HPAKL117_032400101.210765flagellar biosynthesis protein FliP
HPAKL117_032450111.727223iron(III) dicitrate transport protein
HPAKL117_03250-1132.351555ferrous iron transport protein B
HPAKL117_03255-1153.005980hypothetical protein
HPAKL117_032601133.262046acetyl-CoA acetyltransferase
HPAKL117_032650123.509082succinyl-CoA-transferase subunit A
HPAKL117_032702143.755712succinyl-CoA-transferase subunit B
HPAKL117_032753143.595983hypothetical protein
HPAKL117_032801132.892781short-chain fatty acids transporter
HPAKL117_032852132.943693putative outer membrane protein
HPAKL117_032903112.977716hydantoin utilization protein A
HPAKL117_032952101.849937N-methyl hydantoinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_03225PHAGEIV270.015 Gene IV protein signature.
		>PHAGEIV#Gene IV protein signature.

Length = 426

Score = 27.2 bits (60), Expect = 0.015
Identities = 13/59 (22%), Positives = 23/59 (38%), Gaps = 1/59 (1%)

Query: 11 VVLCGAMANTAIADPRIEARGELGKLIGGGVGGFVGDKIGGAIGVPGGPVGIGFGRFVG 69
+++ G + D ++ G G GG D++ + GG GI G +G
Sbjct: 198 ILIEGLIFEVQQGDA-LDFSFAAGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLG 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_03240FLGBIOSNFLIP2762e-96 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 276 bits (708), Expect = 2e-96
Identities = 113/245 (46%), Positives = 162/245 (66%), Gaps = 2/245 (0%)

Query: 1 MRFFIFLILICPLICPLMSADSALPSVNLSLNAPNDPKQLVTTLNVIALLTLLVLAPSLI 60
MR + + + L A + LP + S P + + + +T L P+++
Sbjct: 1 MRRLLSVAPVL-LWLITPLAFAQLPGIT-SQPLPGGGQSWSLPVQTLVFITSLTFIPAIL 58

Query: 61 LVMTSFTRLIVVFSFLRTALGTQQTPPTQILVSLSLILTFFIMEPSLKKAYDTGIKPYMD 120
L+MTSFTR+I+VF LR ALGT PP Q+L+ L+L LTFFIM P + K Y +P+ +
Sbjct: 59 LMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSE 118

Query: 121 KKISYTEAFEKSALPFKEFMLKNTREKDLALFFRIRNLPNPKTPDEVSLSVLIPAFMISE 180
+KIS EA EK A P +EFML+ TRE DL LF R+ N + P+ V + +L+PA++ SE
Sbjct: 119 EKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSE 178

Query: 181 LKTAFQIGFLLYLPFLVIDMVISSILMAMGMMMLPPVMISLPFKILVFILVDGFNLLTEN 240
LKTAFQIGF +++PFL+ID+VI+S+LMA+GMMM+PP I+LPFK+++F+LVDG+ LL +
Sbjct: 179 LKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGS 238

Query: 241 LVASF 245
L SF
Sbjct: 239 LAQSF 243


8HPAKL117_03370HPAKL117_03455Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_03370414-0.755198RNA polymerase factor sigma-54
HPAKL117_033752130.269935putative abc transporter, ATP-binding protein
HPAKL117_03380213-0.519616hypothetical protein
HPAKL117_033852111.051313DNA polymerase III subunits gamma and tau
HPAKL117_033904112.880539hypothetical protein
HPAKL117_033953113.178757hypothetical protein
HPAKL117_034003142.938251hypothetical protein
HPAKL117_034051141.991839hypothetical protein
HPAKL117_034100121.394996L-asparaginase II
HPAKL117_034150130.228475anaerobic C4-dicarboxylate transporter
HPAKL117_03430114-1.900117outer membrane protein
HPAKL117_03435212-1.694128tRNA-dihydrouridine synthase B
HPAKL117_03440311-2.152704tRNA(Ile)-lysidine synthase
HPAKL117_03445211-1.059191hypothetical protein
HPAKL117_0345029-0.359680hypothetical protein
HPAKL117_0345529-0.934735hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_03385IGASERPTASE310.021 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.8 bits (69), Expect = 0.021
Identities = 23/79 (29%), Positives = 33/79 (41%), Gaps = 18/79 (22%)

Query: 501 SKPKPTTETTAET--KENDTKEAVEKETKEIK----------------ENDTKEAVEKKA 542
+ P TTET AE +E+ T E E++ E T E + +
Sbjct: 1031 ATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGS 1090

Query: 543 KEKEVQETQPKETPTALQE 561
+ KE Q T+ KET T +E
Sbjct: 1091 ETKETQTTETKETATVEKE 1109



Score = 30.4 bits (68), Expect = 0.025
Identities = 20/75 (26%), Positives = 29/75 (38%), Gaps = 3/75 (4%)

Query: 481 ALKNQNKSALEVVKEFKFPYSKPKPTTETTAETKENDTKEAVEKETKEIKENDTKEAVEK 540
+ A E K + ++ +ETKE T E E T E KE K EK
Sbjct: 1061 TTAQNREVAKEAKSNVKANTQTNE-VAQSGSETKETQTTETKETATVE-KEEKAKVETEK 1118

Query: 541 KAKEKEVQ-ETQPKE 554
+ +V + PK+
Sbjct: 1119 TQEVPKVTSQVSPKQ 1133


9HPAKL117_04160HPAKL117_04290Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_041602153.325184hydrogenase nickel incorporation protein
HPAKL117_041652153.304842flagellar hook protein FlgE
HPAKL117_041701152.742311CDP-diacylglycerol pyrophosphatase
HPAKL117_041751152.811924alkylphosphonate uptake protein
HPAKL117_041801152.533323hypothetical protein
HPAKL117_041852152.612835hypothetical protein
HPAKL117_041903162.553300catalase
HPAKL117_041952142.129151iron-regulated outer membrane protein
HPAKL117_04200419-1.946665Holliday junction resolvase
HPAKL117_04205622-3.081887hypothetical protein
HPAKL117_04210522-2.764700hypothetical protein
HPAKL117_04215517-2.537122hypothetical protein
HPAKL117_04220314-2.362527hypothetical protein
HPAKL117_04225312-2.062120hypothetical protein
HPAKL117_04230011-0.382712hypothetical protein
HPAKL117_04235-190.382081Holliday junction DNA helicase RuvA
HPAKL117_04240-190.838239hypothetical protein
HPAKL117_04245-191.536728virulence factor MviN
HPAKL117_042500112.286648cysteinyl-tRNA synthetase
HPAKL117_042551112.733306vacuolating cytotoxin
HPAKL117_04260-1141.828960iron(III) dicitrate transporter, ATP-binding
HPAKL117_042650143.934286putative ABC transporter permease
HPAKL117_042700152.629062short-chain oxidoreductase
HPAKL117_042750173.043951hypothetical protein
HPAKL117_04280-1182.798450hypothetical protein
HPAKL117_042850172.778141hypothetical protein
HPAKL117_04290-1183.187433blood group antigen-binding adhesin BabA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04165FLGHOOKAP1427e-06 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 42.3 bits (99), Expect = 7e-06
Identities = 13/49 (26%), Positives = 27/49 (55%)

Query: 669 SISGSKLESSNVDLSRSLTNLIVVQRGFQANSKAVTTSDQILNTLLNLK 717
+S + S V+L NL Q+ + AN++ + T++ I + L+N++
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 39.2 bits (91), Expect = 5e-05
Identities = 11/35 (31%), Positives = 20/35 (57%)

Query: 4 SLWSGVNGMQAHQIALDIESNNIANVNTTGFKYSR 38
+ + ++G+ A Q AL+ SNNI++ N G+
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04250OMS28PORIN300.019 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 29.8 bits (66), Expect = 0.019
Identities = 13/37 (35%), Positives = 25/37 (67%)

Query: 309 EEDLLVSKKRLDKIYRLKQRVLGTLGGINPNFKKEIL 345
+E L+ S++ LD+ + Q+VL + G+NP+ K ++L
Sbjct: 188 KETLMASERALDETVQEAQKVLNMVNGLNPSNKDQVL 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04255VACCYTOTOXIN18630.0 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 1863 bits (4827), Expect = 0.0
Identities = 1049/1333 (78%), Positives = 1137/1333 (85%), Gaps = 70/1333 (5%)

Query: 1 MELQQTHRKINRPIISLALVGALIGTELGANTPNDPIHSESRAFFTTVLIPAIVGGIATG 60
ME+QQTHRKINRP++SLALVGAL+ TP AFFTTV+IPAIVGGIATG
Sbjct: 1 MEIQQTHRKINRPLVSLALVGALVSI-----TP----QQSHAAFFTTVIIPAIVGGIATG 51

Query: 61 TAVGTVSGLLSWGLKQAEQANKTPDKPDKVWRIQAGRGFNNFPNKQYDLYKSLLSSKIDG 120
AVGTVSGLL WGLKQAE+ANKTPDKPDKVWRIQAG+GFN FPNK+YDLYKSLLSSKIDG
Sbjct: 52 AAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAGKGFNEFPNKEYDLYKSLLSSKIDG 111

Query: 121 GWDWGNAARHYWVKDGQWNKLEVGMQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFN 180
GWDWGNAARHYWVKDGQWNKLEV MQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFN
Sbjct: 112 GWDWGNAARHYWVKDGQWNKLEVDMQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFN 171

Query: 181 GNSFTSFKDSADRTTRVNFDAKNILIDNFLEINNRVGSGAGRKASSTVLTLKSSEKITSR 240
GNSFTS+KDSADRTTRV+F+AKNILIDNFLEINNRVGSGAGRKASSTVLTL++SE ITSR
Sbjct: 172 GNSFTSYKDSADRTTRVDFNAKNILIDNFLEINNRVGSGAGRKASSTVLTLQASEGITSR 231

Query: 241 ENAEISLYDGATLNLVSSSNHSVDLYGKVWMGRLQYVGAYLAPSYSTINTSKVVGEMNFR 300
ENAEISLYDGATLNL S+S V L G VWMGRLQYVGAYLAPSYSTINTSKV GE+NF
Sbjct: 232 ENAEISLYDGATLNLASNS---VKLMGNVWMGRLQYVGAYLAPSYSTINTSKVTGEVNFN 288

Query: 301 HLAVGDQNAAQAGIIANKKTNIGVLDLWQSAGLSIIAPPEGGYESKTKD----------- 349
HL VGD NAAQAGIIA+ KT+IG LDLWQSAGL+IIAPPEGGY+ K D
Sbjct: 289 HLTVGDHNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPNDKPSNTTQNNAK 348

Query: 350 ----------------NPKNDTQKTEIQPTQVIDGPFAGAKDTVVNIFRLNTKSDGTLKA 393
NP N QKTEIQPTQVIDGPFAG K+TVVNI R+NT +DGT++
Sbjct: 349 NDKQESSQNNSNTQVINPPNSAQKTEIQPTQVIDGPFAGGKNTVVNINRINTNADGTIRV 408

Query: 394 GGFKASLSTNAAHLNIGEGGINLSNQASGRSLLVENLTGNITVEGALRVNNQVGGSAVAG 453
GGFKASL+TNAAHL+IG+GGINLSNQASGRSLLVENLTGNITV+G LRVNNQVGG A+AG
Sbjct: 409 GGFKASLTTNAAHLHIGKGGINLSNQASGRSLLVENLTGNITVDGPLRVNNQVGGYALAG 468

Query: 454 SSANFEFKAGEDTNNATATFNNDIHLGKAVNLRVDAHTAYFNGNIYLGKSTNLKVNGHSA 513
SSANFEFKAG DT N TATFNNDI LG+ VNL+VDAHTA
Sbjct: 469 SSANFEFKAGTDTKNGTATFNNDISLGRFVNLKVDAHTA--------------------- 507

Query: 514 HFKNIDASKSDNGLNTSALDLSGVTDKVNINKLTTAATNVSIKNFDIKELVVTTRVQSFG 573
+FK ID + G NT LD SGVT+KVNINKL TA+TNV++KNF+I ELVV T S G
Sbjct: 508 NFKGIDTG--NGGFNT--LDFSGVTNKVNINKLITASTNVAVKNFNINELVVKTNGVSVG 563

Query: 574 QYTIFGENIGDKSRIGVVSLQTGYSPAYSGGVTFKSGKKLVIDEIYHAPWNYFDARNVTD 633
+YT F E+IG +SRI V L+TG YSGGV FK G+KLVI++ Y+APWNYFDARN+ +
Sbjct: 564 EYTHFSEDIGSQSRINTVRLETGTRSIYSGGVKFKGGEKLVINDFYYAPWNYFDARNIKN 623

Query: 634 VEINKKILFGAPGYIAGKTGLMFNNLTLNSNASMDYGKDLDLTIQGHFTNNQGVMNLFVQ 693
VEI K+ FG G G LMFNNLTL NA MDY + +LTIQG F NNQG +N V+
Sbjct: 624 VEITNKLAFGPQGSPWGTAKLMFNNLTLGQNAVMDYSQFSNLTIQGDFVNNQGTINYLVR 683

Query: 694 DGRVATLNAGHQASMIFNNVVDSATGFYQPLIKINNAQNLTKNKEHVLVKARNIDYNLVG 753
G+VATLN G+ A+M F+N VDSATGFYQPL+KIN+AQ+L KNKEHVL+KA+ I Y G
Sbjct: 684 GGQVATLNVGNAAAMFFSNNVDSATGFYQPLMKINSAQDLIKNKEHVLLKAKIIGY---G 740

Query: 754 VQGASYDNISASNTNLMEQFKERLALYNNNNRMDICVVRNTDDIKACGMAIGNQAMVNNP 813
A D+I+ N NL+EQFKERLALYNNNNRMDICVVRNTDDIKACG AIGNQ+MVNNP
Sbjct: 741 NVSAGTDSIA--NVNLIEQFKERLALYNNNNRMDICVVRNTDDIKACGTAIGNQSMVNNP 798

Query: 814 DSYKYLIGKAWKNTGISKTANNTTIAVNLGKNSTPTENGGNTTDLPKNTTNKARFASYAL 873
++YKYL GKAWKN GISKTAN + I+V+ NSTPTENGGNTT+LP NTTNK RFASYAL
Sbjct: 799 ENYKYLEGKAWKNIGISKTANGSKISVHYLGNSTPTENGGNTTNLPTNTTNKVRFASYAL 858

Query: 874 IKNAPFAHYNATPNLVAINKHDFGTIESVFELANRSKDIDTLYASSGAQGRDLLQTLLID 933
IKNAPFA Y+ATPNLVAIN+HDFGTIESVFELANRS DIDTLYA+SGAQGRDLLQTLLID
Sbjct: 859 IKNAPFARYSATPNLVAINQHDFGTIESVFELANRSNDIDTLYANSGAQGRDLLQTLLID 918

Query: 934 SHNAGYARTMIDATSANEITKQLNEANSALNDIASLDHKTSSLQTLSLSNAMILNSRLVN 993
SH+AGYARTMIDATSANEITKQLN A + LN+IASL+HKTS LQTLSLSNAMILNSRLVN
Sbjct: 919 SHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLSLSNAMILNSRLVN 978

Query: 994 LSRRHTNNIDSFAKRLQALKGQRFASLESAAEVLYQFAPKYEKPTNVWANAIGGASLNNG 1053
LSRRHTN+IDSFAKRLQALK QRFASLESAAEVLYQFAPKYEKPTNVWANAIGG SLN+G
Sbjct: 979 LSRRHTNHIDSFAKRLQALKDQRFASLESAAEVLYQFAPKYEKPTNVWANAIGGTSLNSG 1038

Query: 1054 GNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFNNQANSLNSGANNTNFGVYSRIFAN 1113
GNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSF+NQANSLNSGANNTNFGVYSRIFAN
Sbjct: 1039 GNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGANNTNFGVYSRIFAN 1098

Query: 1114 QHEFDFEAQGAIGSDQSSLNFKSALLQDLNQSYNYLAYSAAARASYGYDFAFFRNALVLK 1173
QHEFDFEAQGA+GSDQSSLNFKSALL+DLNQSYNYLAYSAA RASYGYDFAFFRNALVLK
Sbjct: 1099 QHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASYGYDFAFFRNALVLK 1158

Query: 1174 PSVGVSYNHLGSTNFESNS-QLVALKNGASSQHLFNASANVEARYYYGDTSYFYMNAGVL 1232
PSVGVSYNHLGSTNF+SNS Q VALKNGASSQHLFNASANVEARYYYGDTSYFYMNAGVL
Sbjct: 1159 PSVGVSYNHLGSTNFKSNSNQKVALKNGASSQHLFNASANVEARYYYGDTSYFYMNAGVL 1218

Query: 1233 QEFANFGSSNAVSLNTFRVNVARNPLNTHARVMMGGELQLAKEVFLNLGFIYAHNLISNA 1292
QEFANFGSSNAVSLNTF+VN RNPLNTHARVMMGGEL+LAKEVFLNLGF+Y HNLISN
Sbjct: 1219 QEFANFGSSNAVSLNTFKVNATRNPLNTHARVMMGGELKLAKEVFLNLGFVYLHNLISNI 1278

Query: 1293 GHFASNLGMRYSF 1305
GHFASNLGMRYSF
Sbjct: 1279 GHFASNLGMRYSF 1291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04270DHBDHDRGNASE865e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 86.3 bits (213), Expect = 5e-22
Identities = 59/243 (24%), Positives = 108/243 (44%), Gaps = 12/243 (4%)

Query: 1 MSEKKESQKVAIITGASSGIGLECALMLLDQGYKVYALSRHATLCVALNNALC------E 54
M+ K K+A ITGA+ GIG A L QG + A+ + + ++L E
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 55 SIDVDVSDSNALKEAFLNISAKEDHCDVLINSAGYGVFGSVEDTPIDEVKKQFGVNFFAL 114
+ DV DS A+ E I + D+L+N AG G + +E + F VN +
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 115 CEVVQFCLPLLKNKPYSKIFNLSSIAGRVSMLFLGHYSASKHALEAYSDALRLELKPFNI 174
+ + ++ I + S V + Y++SK A ++ L LEL +NI
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 175 QVCLIEPGPVKSNWEKTAFSVESFESENSLYALEVNAAKSFYSGV-YQKALSPKAVAQKI 233
+ ++ PG +++ + + ++ EN + + ++F +G+ +K P +A +
Sbjct: 181 RCNIVSPGSTETDMQWSLWA-----DENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAV 235

Query: 234 VFL 236
+FL
Sbjct: 236 LFL 238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04285SECA290.003 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.5 bits (66), Expect = 0.003
Identities = 13/69 (18%), Positives = 33/69 (47%), Gaps = 12/69 (17%)

Query: 4 SPTKKDYTQYSEKQLFNLINQLEQKIKKMQNDRASFKEKMAKELEKRDQNFKDKIDALNE 63
S + + +++ N+IN +E +++K+ ++ EL+ + F+ +++
Sbjct: 12 SRNDRTLRRM--RKVVNIINAMEPEMEKLSDE----------ELKGKTAEFRARLEKGEV 59

Query: 64 LLQKISQAF 72
L I +AF
Sbjct: 60 LENLIPEAF 68


10HPAKL117_04680HPAKL117_04785Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_04680317-0.813745cell division protein FtsA
HPAKL117_04685418-1.975279cell division protein FtsZ
HPAKL117_04690417-3.201725IS606 transposase
HPAKL117_04695316-3.075825transposase
HPAKL117_04700013-3.714348transposase A-OrfA
HPAKL117_04705113-3.651558hypothetical protein
HPAKL117_04710214-4.122030hypothetical protein
HPAKL117_04715215-4.694541exodeoxyribonuclease VII large subunit
HPAKL117_04730216-4.516936hypothetical protein
HPAKL117_04735316-5.074400hypothetical protein
HPAKL117_04740419-5.060942hypothetical protein
HPAKL117_04745320-5.435389hypothetical protein
HPAKL117_04750220-5.253584hypothetical protein
HPAKL117_04755220-4.739902hypothetical protein
HPAKL117_04760222-5.745425hypothetical protein
HPAKL117_04765325-4.984381serine/threonine kinase
HPAKL117_04770425-4.608683serine/threonine kinase
HPAKL117_04775326-5.897371protein phosphatase 2 C-like protein (ptc1)
HPAKL117_04780626-4.757686hypothetical protein
HPAKL117_04785428-2.634206hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04680SHAPEPROTEIN418e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 40.9 bits (96), Expect = 8e-06
Identities = 39/181 (21%), Positives = 69/181 (38%), Gaps = 13/181 (7%)

Query: 211 AASIATLSNDERELGVACVDIGGETCNITIYSGNSIRYNKYLPIGSHHLSTDLSSMLNTP 270
AA+I G VDIGG T + + S N + Y+ + IG + + +
Sbjct: 146 AAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRN 205

Query: 271 F------PYAEEVKIKYGDLSFESGIETPSQSVQIPTTGSDGNESHVVPLSKIQNIMRDR 324
+ AE +K + G S G E V+ + ++I +++
Sbjct: 206 YGSLIGEATAERIKHEIG--SAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEP 263

Query: 325 ALETFQIIHRSIQDSGLE---EHLGGGVVLTGGMALMKGIKELAKAHFTNYPVRLAA-PM 380
+ +++ E + G+VLTGG AL++ + L T PV +A P+
Sbjct: 264 LTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLL-MEETGIPVVVAEDPL 322

Query: 381 E 381

Sbjct: 323 T 323


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04740GPOSANCHOR542e-09 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 53.5 bits (128), Expect = 2e-09
Identities = 47/326 (14%), Positives = 111/326 (34%), Gaps = 13/326 (3%)

Query: 3 ENEELELETKVAELDKRAAELNEREKQLNKRQKQLKTDIDAFNQKKEVYLKNQKEFEDFM 62
+ + E + L + ++L+ K L +L ++ +K K+ E +
Sbjct: 56 QERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKI 115

Query: 63 EDREVLEKELDQKVKENLRTYEKEQKELILERIRKQLQEQQESLNKDYGKKLENIKEHSD 122
++ E + +L++ ++ + + ++ K +++ + + N
Sbjct: 116 QELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADS 175

Query: 123 RLENDLKT----------QFDAQLERWEQNFKGYEQQLTGILNDKALLDLDKECFNAQKQ 172
L+ + + LE ++ + +KA L K +
Sbjct: 176 AKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALE 235

Query: 173 AFEKKMKQREEEIKKENRNEIERLNKEKNGLHRQLDEMADETSDLRQKNRELREKIGFQK 232
+IK E L + L + L+ + ++ K + L +
Sbjct: 236 GAMNFSTADSAKIKTLEA-EKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKA-AL 293

Query: 233 DYDMEKLEEYNRVLEKCKEDLLAANENLKNKILELESEKNKLDP-RDERIKELEEKEREL 291
+ + LE ++VL ++ L + + +LE+E KL+ + R+L
Sbjct: 294 EAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDL 353

Query: 292 EGIRAQKEDAEQKCARLSAYNVSLEA 317
+ R K+ E + +L N EA
Sbjct: 354 DASREAKKQLEAEHQKLEEQNKISEA 379



Score = 39.7 bits (92), Expect = 3e-05
Identities = 53/293 (18%), Positives = 104/293 (35%), Gaps = 15/293 (5%)

Query: 3 ENEELELETKVAELDKRAAELNEREKQLNKRQKQLKTDIDAFNQKKEVYLKNQKEFEDFM 62
E E+ L + A+L+K + + K L+ + A ++ K + +F
Sbjct: 147 EAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFS 206

Query: 63 EDREVLEKEL-DQKVKENLRTYEKEQKELILERIRKQLQEQQESLNKDYGKKLENIKEHS 121
K L +K R + E+ + ++L + E
Sbjct: 207 TADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELE 266

Query: 122 DRLE--NDLKTQFDAQLERWEQNFKGYEQQLTGILNDKALLDLDKECFNAQKQAFEKKMK 179
LE + T A+++ E E + + + +L+ +++ A + K
Sbjct: 267 KALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKK 326

Query: 180 QREEEIKKENRNEIERLNKEKNGLHRQLDEMADETSDLRQKNRELREKIGFQKDYDMEKL 239
Q E E +K + + + L R LD + L ++++L E+ +
Sbjct: 327 QLEAEHQKL-EEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISE------- 378

Query: 240 EEYNRVLEKCKEDLLAANENLKNKILELESEKNKLDPRDERIKELEEKERELE 292
+ + DL A+ E K LE +KL ++ KELEE ++ E
Sbjct: 379 ----ASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKKLTE 427



Score = 37.0 bits (85), Expect = 2e-04
Identities = 36/260 (13%), Positives = 84/260 (32%), Gaps = 7/260 (2%)

Query: 74 QKVKENLRTYEKEQKELILE-RIRKQLQEQQESLNKDYGKKLENIKEHSDRLENDLKTQF 132
+KV+E +E E L L+ + + N + ++L N KE + + L +
Sbjct: 53 EKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEK- 111

Query: 133 DAQLERWEQNFKGYEQQLTGILNDKALLDLDKECFNAQKQAFEKKMKQREEEIKKENRNE 192
++++ E E+ L G +N + A+K A + E+ ++ N
Sbjct: 112 ASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALE-GAMNF 170

Query: 193 IERLNKEKNGLHRQLDEMADETSDLRQKNRELREKIGFQKDYDMEKLEEYNRVLEKCKED 252
+ + L + + ++L + K++
Sbjct: 171 STADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADS----AKIKTLEAEKAALAAR 226

Query: 253 LLAANENLKNKILELESEKNKLDPRDERIKELEEKERELEGIRAQKEDAEQKCARLSAYN 312
+ L+ + ++ K+ + LE ++ ELE + +
Sbjct: 227 KADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTL 286

Query: 313 VSLEADLNILNERFENLKNI 332
+ +A L E+ +
Sbjct: 287 EAEKAALEAEKADLEHQSQV 306


11HPAKL117_05115HPAKL117_05190Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_051152140.392118UDP-glucose 4-epimerase
HPAKL117_051252101.368765**putative outer membrane protein
HPAKL117_051301121.393389secreted protein
HPAKL117_05135191.283366cysteine-rich protein C
HPAKL117_05140182.190570hypothetical protein
HPAKL117_05145081.8991232-keto-3-deoxy-6-phosphogluconate aldolase
HPAKL117_05150-291.365453phosphogluconate dehydratase
HPAKL117_051551100.545144glucose-6-phosphate 1-dehydrogenase
HPAKL117_051602111.0956166-phosphogluconolactonase
HPAKL117_051653111.465659glucokinase
HPAKL117_051704120.663153NADP-dependent alcohol dehydrogenase
HPAKL117_051751111.662427lipopolysaccharide 1,3-galactosyltransferase
HPAKL117_051802112.719474hypothetical protein
HPAKL117_051850143.318931outer membrane protein
HPAKL117_05190-1133.087395pyruvate flavodoxin oxidoreductase subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_05115NUCEPIMERASE1194e-33 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 119 bits (299), Expect = 4e-33
Identities = 62/341 (18%), Positives = 132/341 (38%), Gaps = 48/341 (14%)

Query: 1 MALLFTGACGYIGSHTARAFLEKTKENIVIVDDLSTGF---LEHLKALEHYYPNRVVFVQ 57
M L TGA G+IG H ++ LE + +V +D+L+ + L+ + P F +
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQ-VVGIDNLNDYYDVSLKQARLELLAQPG-FQFHK 58

Query: 58 ANLNETQKLDAFLNKQQLKDPIEAILHFGAKISVEESTRLPLEYYTNNTLNTLELVKLCL 117
+L + + + E + +++V S P Y +N L +++ C
Sbjct: 59 IDLADREGMTDLFASGH----FERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCR 114

Query: 118 KHAIKRFIFSSTAVVYGESDS-SLNEESPLN-PINPYGASKMMSERILLDTSKIADFNCV 175
+ I+ +++S++ VYG + + + ++ P++ Y A+K +E + S +
Sbjct: 115 HNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPAT 174

Query: 176 ILRYFNVAGACMHNNYTTPYTLGQRTLNATHLIKIACECAVGKRKKMGIFGTNYPTRDGT 235
LR+F V G + + + L GK ++ G
Sbjct: 175 GLRFFTVYGPWGRPD-MALFKFTKAMLE-------------GKSID--VYN------YGK 212

Query: 236 CIRDYIHVDDLANAHLASYQTLLEKNKS---------------EIYNVGYNQGHSVKEVI 280
RD+ ++DD+A A + + + +YN+G + + + I
Sbjct: 213 MKRDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYI 272

Query: 281 EKVKEISNNDFLVEILDKRQGDPASLIANNAKILQNTSFKP 321
+ +++ + +L + GD A+ + + F P
Sbjct: 273 QALEDALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTP 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_05190YERSSTKINASE290.011 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 28.9 bits (64), Expect = 0.011
Identities = 13/33 (39%), Positives = 22/33 (66%)

Query: 80 IENIFANEKEDTTYIITSYLNKEELFEKKPELK 112
+ N+ A+EK D ++++ L+ E FEK PE+K
Sbjct: 314 VGNLGASEKSDVFLVVSTLLHCIEGFEKNPEIK 346


12HPAKL117_05275HPAKL117_05310Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_05275315-0.789532flgM protein
HPAKL117_05280313-1.881405hypothetical protein
HPAKL117_05285413-1.733335FKBP-type peptidyl-prolyl cis-trans isomerase
HPAKL117_05290314-2.445268hypothetical protein
HPAKL117_05295414-2.103278peptidoglycan-associated lipoprotein precursor
HPAKL117_053002150.046966translocation protein TolB
HPAKL117_053052160.031839hypothetical protein
HPAKL117_053102142.481643Biopolymer transport protein ExbD/TolR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_05290GPOSANCHOR320.002 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 32.3 bits (73), Expect = 0.002
Identities = 25/145 (17%), Positives = 49/145 (33%)

Query: 27 GATKKELKQLQINSKNFSNILTKIHSQVEANTQAQEGLRSVYEGQANKIKDLNNAILSQE 86
K + ++ +A EG + + KIK L + E
Sbjct: 200 EGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALE 259

Query: 87 ESLRALKALQEVQANTLKQQSQTLEDLRNEIHANQQAIQQLDKQNKEMSELLTKLSQDLV 146
L+ E N S ++ L E A + L+ Q++ ++ L +DL
Sbjct: 260 ARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLD 319

Query: 147 SQIALIQKALKEQEEKAEKSLKSNA 171
+ ++ E ++ E++ S A
Sbjct: 320 ASREAKKQLEAEHQKLEEQNKISEA 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_05295OMPADOMAIN1433e-44 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 143 bits (363), Expect = 3e-44
Identities = 44/172 (25%), Positives = 69/172 (40%), Gaps = 26/172 (15%)

Query: 22 NMDKETVAGDVSAKAVQSAPVSTETVQEKQEPKEEPKQEPAPVVEKKPAIESGTIIASIY 81
D ++ VS + Q P P PAP V+ K T+ + +
Sbjct: 177 RPDNGMLSLGVSYRFGQGEAA----------PVVAPAPAPAPEVQTK----HFTLKSDVL 222

Query: 82 FDFDKYEIKESDQETLDEIVQKAKE---NHMQVLLEGNTDEFGSSEYNQALGVKRTLSVK 138
F+F+K +K Q LD++ + V++ G TD GS YNQ L +R SV
Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVV 282

Query: 139 NALVIKGVEKDMIKTISFGEIKPKCTQKT---------KECYKENRRVDVKL 181
+ L+ KG+ D I GE P +C +RRV++++
Sbjct: 283 DYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_05305GPOSANCHOR290.025 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 28.9 bits (64), Expect = 0.025
Identities = 15/123 (12%), Positives = 34/123 (27%), Gaps = 6/123 (4%)

Query: 60 EKKEEKVAEKVEEKKEEKKEEKKDEKVVEKNATDKEGDFIDPKEQEESLENIFSSL-NDF 118
E ++ + + E ++ + E + ++ LE N
Sbjct: 182 EAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFS 241

Query: 119 QEKTDTNAQKDEQKNEQEEEQRRLKEQQRLRKNQKNQEM-----LKGLQQNLDQFAQKLE 173
+ + +K E Q L++ N + L+ + L+ LE
Sbjct: 242 TADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLE 301

Query: 174 SVK 176

Sbjct: 302 HQS 304


13HPAKL117_06590HPAKL117_06765Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_06590211-2.128868prephenate dehydrogenase
HPAKL117_06595614-6.057298putative endonuclease
HPAKL117_06600815-6.801203cag pathogenicity island protein Y
HPAKL117_06605816-7.063282chromosome partitioning protein
HPAKL117_06610617-6.519025hypothetical protein
HPAKL117_06615519-7.119889hypothetical protein
HPAKL117_06620423-8.124503hypothetical protein
HPAKL117_06625225-7.566875hypothetical protein
HPAKL117_06630326-7.629659hypothetical protein
HPAKL117_06635327-8.231318conjugal transfer protein TraG
HPAKL117_06640532-10.454939hypothetical protein
HPAKL117_06645530-9.691364hypothetical protein
HPAKL117_06650430-9.579058hypothetical protein
HPAKL117_06655426-7.776358VirB11 type IV secretion ATPase
HPAKL117_06660520-6.482173hypothetical protein
HPAKL117_06665421-6.238002hypothetical protein
HPAKL117_06670422-5.962313hypothetical protein
HPAKL117_06675522-6.653984hypothetical protein
HPAKL117_06680524-7.448148competence protein
HPAKL117_06685725-8.051256VirB9 type IV secretion protein
HPAKL117_06690729-8.745284hypothetical protein
HPAKL117_06695829-9.372855hypothetical protein
HPAKL117_06700828-9.256355topoisomerase I
HPAKL117_06705727-9.736376DNA transfer protein
HPAKL117_06710625-8.372850hypothetical protein
HPAKL117_06715524-6.810266hypothetical protein
HPAKL117_06720215-5.209552hypothetical protein
HPAKL117_06725015-1.904451hypothetical protein
HPAKL117_06730-115-0.652371hypothetical protein
HPAKL117_06735-116-0.035191hypothetical protein
HPAKL117_06740-117-0.418894hypothetical protein
HPAKL117_06745-116-0.820952hypothetical protein
HPAKL117_06750-114-2.107360hypothetical protein
HPAKL117_06755116-3.7834667-cyano-7-deazaguanine reductase
HPAKL117_06760-215-3.224476iojap-related protein
HPAKL117_06765-115-3.221533tRNA delta(2)-isopentenylpyrophosphate
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_06690PF04335982e-26 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 98.4 bits (245), Expect = 2e-26
Identities = 35/202 (17%), Positives = 75/202 (37%), Gaps = 18/202 (8%)

Query: 94 AERKIGDWIFSSAVFFFALALIEAIIIICLLPLKEKVPYLVTFSNATQNFAIVQR--ADK 151
+K+ + A ALA + + L PLK PY++T T +I + D
Sbjct: 30 RSKKLAWVV---AGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLHGDA 86

Query: 152 SIRANQALIRQLVASYVNNRE--NISNIKEQNEIAHETIRLQSAFEVWDFFEKLVSYEH- 208
+I ++A+ + +A+YV RE + +E + + + SA D + + ++
Sbjct: 87 TITYDEAVRKYFLATYVRYREGWIAAAREEY----FDAVMVMSARPEQDRWSRFYKTDNP 142

Query: 209 ----SIYTNINLTRKISIINIALISKTQANIEISAQLFNKEKLESEKRYRIIMTFEFEPI 264
+I N + I ++ + A + + + ++ + ++ +
Sbjct: 143 QSPQNILAN-RTDVFVEIKRVSFLGGNVAQVYFT-KESVTGSNSTKTDAVATIKYKVDGT 200

Query: 265 EIDTKSVPLNPTGFIVTGYDVT 286
NP G+ V Y
Sbjct: 201 PSKEVDRFKNPLGYQVESYRAD 222


14HPAKL117_07035HPAKL117_07120Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_07035290.343852ABC transporter permease protein
HPAKL117_0704029-0.446141outer membrane protein
HPAKL117_07045210-0.400618branched-chain amino acid aminotransferase
HPAKL117_07050211-1.099753outer membrane protein
HPAKL117_07055212-1.343896DNA polymerase I
HPAKL117_07060118-0.680340type II restriction enzyme
HPAKL117_07065218-0.081578type II restriction modification enzyme
HPAKL117_070703191.136175hypothetical protein
HPAKL117_070753120.600982thymidylate kinase
HPAKL117_070804120.205010phosphopantetheine adenylyltransferase
HPAKL117_070854110.3078703-octaprenyl-4-hydroxybenzoate carboxy-lyase
HPAKL117_070903120.213867hypothetical protein
HPAKL117_070953130.295811flagellar basal body P-ring biosynthesis protein
HPAKL117_071002120.409128DNA helicase II
HPAKL117_071052130.630470hypothetical protein
HPAKL117_071101131.074121seryl-tRNA synthetase
HPAKL117_071151150.450721hypothetical protein
HPAKL117_071202140.154556exodeoxyribonuclease VII small subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07060V8PROTEASE280.049 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 28.4 bits (63), Expect = 0.049
Identities = 12/43 (27%), Positives = 19/43 (44%), Gaps = 5/43 (11%)

Query: 226 NTIKIHDAQIENSYPYVVRAATNNGIKGFI---IDDPTFANEK 265
I IH + N + V N ++ F+ I+D FAN+
Sbjct: 247 EVIGIHWGGVPNEFNGAVF--INENVRNFLKQNIEDIHFANDD 287


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07080LPSBIOSNTHSS2233e-78 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 223 bits (570), Expect = 3e-78
Identities = 64/148 (43%), Positives = 94/148 (63%)

Query: 4 IGIYPGTFDPVTNGHIDIIHRSSELFEKLIVAVAHSSAKNPMFSLKERLEMMQLATKGFK 63
IYPG+FDP+T GH+DII R LF+++ VAV + K PMFS++ERLE + A
Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61

Query: 64 NVECVAFEGLLANLAKEYHCKVLVRGLRVVSDFEYELQMGYANKSLNHELETLYFMPTLQ 123
N + +FEGL N A++ ++RGLRV+SDFE ELQM NK+L +LET++ + +
Sbjct: 62 NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTSTE 121

Query: 124 NAFISSSIVRSIIAHKGDASHLVPKEIH 151
+F+SSS+V+ + G+ H VP +
Sbjct: 122 YSFLSSSLVKEVARFGGNVEHFVPSHVA 149


15HPAKL117_07185HPAKL117_07365Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_07185113-4.764269transaldolase
HPAKL117_07190214-3.69149750S ribosomal protein L25/general stress protein
HPAKL117_07195314-3.918057peptidyl-tRNA hydrolase
HPAKL117_07200112-2.980842hypothetical protein
HPAKL117_07205112-2.937195type II methylase
HPAKL117_07210211-1.533131DNA adenine methylase
HPAKL117_072252110.728443hypothetical protein
HPAKL117_072301110.581665hypothetical protein
HPAKL117_072350110.890425putative cation transporting P-type ATPase
HPAKL117_072401101.526453hypothetical protein
HPAKL117_072451111.502696riboflavin biosynthesis protein (ribG)
HPAKL117_072501111.535336sodium/glutamate symport carrier
HPAKL117_072552132.745603saccharopine dehydrogenase
HPAKL117_072600131.863077ferrodoxin-like protein
HPAKL117_07265-1111.626397putative glycerol-3-phosphate acyltransferase
HPAKL117_07270-2110.022178dihydroneopterin aldolase
HPAKL117_07275-28-0.553222FrpB-like protein
HPAKL117_07280-27-0.856732iron-regulated outer membrane protein
HPAKL117_0728508-3.441081selenocysteine synthase
HPAKL117_0729019-3.368020transcription elongation factor NusA
HPAKL117_0729519-3.822663putative type IIS restriction enzyme R and M
HPAKL117_07310110-2.143002type III R-M system restriction enzyme
HPAKL117_07315113-1.116725ATP-dependent DNA helicase RecG
HPAKL117_07320014-0.796402hypothetical protein
HPAKL117_07325013-0.704876outer membrane protein
HPAKL117_073300110.091149exodeoxyribonuclease III
HPAKL117_073351120.370728*periplasmic competence protein
HPAKL117_073402130.330160chromosomal replication initiation protein
HPAKL117_07345116-1.745045purine nucleoside phosphorylase
HPAKL117_07350113-1.701536hypothetical protein
HPAKL117_07355113-1.743874glucosamine--fructose-6-phosphate
HPAKL117_07360-114-3.160876FAD-dependent thymidylate synthase
HPAKL117_07365-114-3.398380hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07270ARGDEIMINASE260.034 Bacterial arginine deiminase signature.
		>ARGDEIMINASE#Bacterial arginine deiminase signature.

Length = 409

Score = 26.3 bits (58), Expect = 0.034
Identities = 12/60 (20%), Positives = 25/60 (41%), Gaps = 8/60 (13%)

Query: 7 VHIHNLVFETILGILEFERLKPQKISVNLDLFYTELPN-----KAYLDYIKIQELIQKMM 61
+I +L+ E ++ + L+ + IS + + K Y + I +I KM+
Sbjct: 70 EYIEDLISEVLVSSVA---LENKFISQFILEAEIKTDFTINLLKDYFSSLTIDNMISKMI 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07340HTHFIS363e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 35.6 bits (82), Expect = 3e-04
Identities = 9/51 (17%), Positives = 25/51 (49%), Gaps = 4/51 (7%)

Query: 125 TVYEIAKKVAQSDTPPYNPVLFYGGTGLGKTHILNAIGNHALQKHKKVVLV 175
+Y + ++ Q+D ++ G +G GK + A+ ++ +++ V +
Sbjct: 148 EIYRVLARLMQTDLT----LMITGESGTGKELVARALHDYGKRRNGPFVAI 194


16HPAKL117_07425HPAKL117_07540Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_07425310-3.022135putative inorganic polyphosphate/ATP-NAD kinase
HPAKL117_07430510-4.112703DNA repair protein (recN)
HPAKL117_07435314-5.901399fibronectin/fibrinogen-binding protein
HPAKL117_07440219-4.080352hypothetical protein
HPAKL117_07445418-4.543435hypothetical protein
HPAKL117_07450318-3.781861hypothetical protein
HPAKL117_07455019-0.879427hypothetical protein
HPAKL117_074601160.023151cag pathogenicity island protein Y VirB10-like
HPAKL117_07465214-1.657395putative type II methylase protein
HPAKL117_07470214-2.431436hypothetical protein
HPAKL117_07475316-3.741174fructose-1,6-bisphosphatase
HPAKL117_07480416-3.367380ribulose-phosphate 3-epimerase
HPAKL117_07485418-4.121125DNA polymerase III subunit epsilon
HPAKL117_07490521-4.949452hypothetical protein
HPAKL117_07495527-7.587840hypothetical protein
HPAKL117_07500424-6.830966hypothetical protein
HPAKL117_07505320-4.520281hypothetical protein
HPAKL117_07510222-5.871910hypothetical protein
HPAKL117_07515219-5.544398hypothetical protein
HPAKL117_07520117-4.788023integrase-recombinase protein
HPAKL117_07525014-3.070633putative ribonuclease N
HPAKL117_07530013-2.116371biotin synthase
HPAKL117_07535415-3.425162hypothetical protein
HPAKL117_07540211-2.139878hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07435FbpA_PF058331126e-29 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 112 bits (282), Expect = 6e-29
Identities = 73/361 (20%), Positives = 147/361 (40%), Gaps = 31/361 (8%)

Query: 97 AKDLAYKSENFILRLEMIPKKANLMILDKEKCVIEA--FRFNDRVAKNDILGALPPN-TY 153
+ ++ ++ +N + L + K + + I++ F FN N +G N
Sbjct: 209 SSEICFRLKNNSIDLSLSNLKEIVEVCKDLFKEIQSNKFEFNCYTKNNSFVGFYCLNLMS 268

Query: 154 EHQEEDLDFKGLLDILEKDFLSYQYKE----LEHKKNHIIKRLNMQKERLKEKLEKLEDP 209
+ + + + +LE +F Y K+ L+ K + + K + R +K + L +
Sbjct: 269 KEDYKKIQYDSSSKLLE-NF--YYAKDKSDRLKSKSSDLQKIVMNNINRCTKKDKILNNT 325

Query: 210 KNLQLEAKELQTQASLLLTYQHLINKHESRVVLKDFED---KECAIEIDKSMPLNAFINK 266
+ + LL + + K S + L ++ I +D++ + +
Sbjct: 326 LKKCEDKDIFKLYGELLTANIYALKKGLSHIELANYYSENYDTVKITLDENKTPSQNVQS 385

Query: 267 KFTLSKKKKQKSQFLYLEEENLKEKIAFKENQINYVKGA---------KEESVLEMFM-- 315
+ K K+ + + +E++ + + + + A K+E + ++
Sbjct: 386 YYKKYNKLKKSEEAANEQLLQNEEELNYLYSVLTNINNADNYDEIEEIKKELIETGYIKF 445

Query: 316 -PVKNSKTKRPMSGYEVLYYKDFKIGLGKNQKENIKL-LQDARANDLWMHVRDIPGSHLI 373
+ SK + + I +GKN +N L L+ A +D+W H ++IPGSH+I
Sbjct: 446 KKIYKSKKSKTSKPMHFISKDGIDIYVGKNNIQNDYLTLKFANKHDIWFHTKNIPGSHVI 505

Query: 374 VFCQKNAPKDEVIMELAKMLIKMQKDVFNS-YEIDYTQRKFVKIIKGAN---VIYSKYRT 429
V + P + ++E A + K +S +DYT+ K VK GA VIYS +T
Sbjct: 506 VKNIMDIP-ESTLLEAANLAAYYSKSQNSSNVPVDYTEVKNVKKPNGAKPGMVIYSTNQT 564

Query: 430 I 430
I
Sbjct: 565 I 565



Score = 35.6 bits (82), Expect = 4e-04
Identities = 20/104 (19%), Positives = 51/104 (49%), Gaps = 7/104 (6%)

Query: 36 KEKHAFVVDLHA--PYIGLSKKPPESVLKNTLALDFCLSKFTKNAKILQANIIDNDRI-- 91
+ ++ + P I L+ + +K + L K+ NAKI+ + I+ DRI
Sbjct: 43 RLSFKLLISSSSNYPRIHLTDLTKPNPIKAPMFCMV-LRKYISNAKIVDIHQINQDRIVV 101

Query: 92 LEITGAKDLAYKSENFILRLEMIPKKANLMILDK-EKCVIEAFR 134
++ +L + S + L +E++ + +N+ ++ K + ++++ +
Sbjct: 102 IDFESTDELGFNSI-YSLIIEIMGRHSNMTLIRKRDNIIMDSIK 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07445TCRTETB250.029 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 25.2 bits (55), Expect = 0.029
Identities = 9/42 (21%), Positives = 18/42 (42%)

Query: 25 ILGVMEELCDTLNDSLNFKKVVCMGIKVSIAFKFLIFCSQSF 66
+ + L+D L K+++ GI ++ + F SF
Sbjct: 61 TFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF 102


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07490FbpA_PF05833320.032 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 31.8 bits (72), Expect = 0.032
Identities = 29/169 (17%), Positives = 61/169 (36%), Gaps = 22/169 (13%)

Query: 118 RNKEFFSKEIEILTYTNSLEELKEQGLEIQLTHHHETHKKTLENGNEIAKEYDYLKDIYQ 177
R + L + K G E+ + + L+ G + +Y + Y
Sbjct: 314 RCTKKDKILNNTLKKCEDKDIFKLYG-ELLTANIYA-----LKKGLSHIELANYYSENYD 367

Query: 178 EVERTRDGELVREIIPSISSTEYFKLYNKLPFESINNENTKLNTNNTIKNTIETNTSIGN 237
V+ T D PS + Y+K YNKL + ++ N + E +
Sbjct: 368 TVKITLDENKT----PSQNVQSYYKKYNKL-------KKSEEAANEQLLQNEEELNYL-Y 415

Query: 238 NIIQNNDNNNPNLSIADLELEQQNLG----EQNGKERTNRADEPNRTRA 282
+++ N +N + I +++ E G ++ K + ++ +P +
Sbjct: 416 SVLTNINNADNYDEIEEIKKELIETGYIKFKKIYKSKKSKTSKPMHFIS 464



Score = 31.4 bits (71), Expect = 0.042
Identities = 35/174 (20%), Positives = 65/174 (37%), Gaps = 23/174 (13%)

Query: 1106 YKYELQALEDKSLDLSQALNQAIEKLPKGVYQYHKTTLKTDALIIDANNERYQEIQKLIK 1165
K + L+ KS DL + + I + K + T K + + + ++ +L+
Sbjct: 290 AKDKSDRLKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCE------DKDIFKLYGELLT 343

Query: 1166 ----NLERG-ELVKWDNLYFQLEQNNEMGIFLKPTKINSKVQDSRLKAYFKIKDALNDL- 1219
L++G ++ N Y E + + I L K S+ S K Y K+K +
Sbjct: 344 ANIYALKKGLSHIELANYY--SENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAAN 401

Query: 1220 -----TSAELSPLSS---DLELESKRVRLNLVYDEFVKKFGYLNENKNRKDIKQ 1265
EL+ L S ++ + + E ++ GY+ K K K
Sbjct: 402 EQLLQNEEELNYLYSVLTNINNADNYDEIEEIKKELIET-GYIKFKKIYKSKKS 454


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07505CABNDNGRPT404e-05 NodO calcium binding signature.
		>CABNDNGRPT#NodO calcium binding signature.

Length = 479

Score = 39.6 bits (92), Expect = 4e-05
Identities = 24/115 (20%), Positives = 36/115 (31%), Gaps = 9/115 (7%)

Query: 146 TNDPMYANTPFSNNPNSPNDNAINGKDGANGSNGYGINGNDGINGSSGSNGANGNNSNHN 205
N + P + AI GAN + + G N ++ + +S+
Sbjct: 232 NETGADYNGHYGGAPMIDDIAAIQRLYGANMTTR-TGDSVYGFNSNTDRDFYTATDSSKA 290

Query: 206 AVGS-----GIDTDGVLGVDG---VNGSSSSSGGSVGGYENNFTNHGSTNNNIGG 252
+ S G DT G +N + S G N HG T N G
Sbjct: 291 LIFSVWDAGGTDTFDFSGYSNNQRINLNEGSFSDVGGLKGNVSIAHGVTIENAIG 345


17HPAKL117_00175HPAKL117_00200N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_00175-2120.202294competence protein
HPAKL117_00180-2110.560110type IV secretion system protein VirB9
HPAKL117_00185-2111.292889comB10 competence protein
HPAKL117_00190-2121.158676mannose-1-phosphate guanyltransferase
HPAKL117_00195-1131.168343GDP-D-mannose dehydratase
HPAKL117_00200-1140.731383nodulation protein (nolK)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00175PF043351289e-39 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 128 bits (324), Expect = 9e-39
Identities = 39/203 (19%), Positives = 75/203 (36%), Gaps = 6/203 (2%)

Query: 40 QSVFRLERNRLKIAYKLLGLMSFIALVLAIVLISVLPLQKTEHHF--VDFLNQDKHYAII 97
+ K+A+ + G+ +A + + ++ PL+ E + VD + A
Sbjct: 22 RDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAK 81

Query: 98 QRADKSISSNEALARSLIGAYVLNRESINRIDDKSRYELVRLQSSSKVWQRFEDLIKTKN 157
D +I+ +EA+ + + YV RE + ++ V + S+ R+ KT N
Sbjct: 82 LHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARPEQDRWSRFYKTDN 141

Query: 158 SIYAQSHLEREVYI-VNIAIYQQDNNPIASVSIAAKLMNENKLVYEKRYKIA-LSYLFDT 215
Q+ L + V I +A V + + + K +A + Y D
Sbjct: 142 PQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNST--KTDAVATIKYKVDG 199

Query: 216 PDFDYASMPKNPTGFKITRYSIT 238
KNP G+++ Y
Sbjct: 200 TPSKEVDRFKNPLGYQVESYRAD 222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00180TYPE4SSCAGX310.010 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 30.5 bits (68), Expect = 0.010
Identities = 28/96 (29%), Positives = 43/96 (44%), Gaps = 10/96 (10%)

Query: 171 IKENTLEKAPTNNKPLKEEKEET----KEKEEETITIGDNTNAMKIVKKDIQKGYRALKS 226
IK+ L N+ + E+ K +EE+ I D A+ + Q + ALK
Sbjct: 340 IKQENLNTTAYINRVMMASNEQIINKEKIREEKQKIILDQAKAL-----ETQYVHNALKR 394

Query: 227 SQ-RKWYCLWICSKKSKLSLMPEEIFNDKQFTYFKF 261
+ + Y + +K +MP EIF+D FTYF F
Sbjct: 395 NPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGF 430


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00195NUCEPIMERASE882e-21 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 87.5 bits (217), Expect = 2e-21
Identities = 46/180 (25%), Positives = 72/180 (40%), Gaps = 19/180 (10%)

Query: 7 LITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGD 66
L+TG G G ++++ LL G++V G+ + + S E L F H D
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQP---GFQFHKID 60

Query: 67 MTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMRILGLEK 126
+ D + L A+ ++ + V+ S E P A+++ G L ILE R ++
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ- 119

Query: 127 KTRFYQASTSELYGEVLETPQNENTPF-------NPRSPYAVAKMYAFYITKNYREAYNL 179
AS+S +YG N PF +P S YA K + Y Y L
Sbjct: 120 --HLLYASSSSVYGL------NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_00200NUCEPIMERASE505e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 49.8 bits (119), Expect = 5e-09
Identities = 51/346 (14%), Positives = 106/346 (30%), Gaps = 54/346 (15%)

Query: 5 ILITGAYGMVGQNTALYFKKNKPDV-----------TLLTPKKSELY-----------LL 42
L+TGA G +G + + + V L + EL L
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDLA 62

Query: 43 DKDNVQAYLKEYKPTGIIHCAGRVGGIVANMNDLSTYMVENLLMGLYLFSSALDLGVKKA 102
D++ + + R + ++ + Y NL L + ++
Sbjct: 63 DREGMTDLFASGHFERVFISPHR-LAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 103 INLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLV 162
+ +SS Y P D ++ + YA K + S G+ L
Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVSL----YAATKKANELMAHTYSHLYGLPATGLR 177

Query: 163 PCNLYGEFDKFEEKIAHMIPGLIARMHTAKLKNEKEFVMWGDGTARREYLNAKDLARFIS 222
+YG + + P + T + K ++ G +R++ D+A I
Sbjct: 178 FFTVYGPWGR---------PDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAII 228

Query: 223 LAYENIASIPS-----------------VMNVGSGVDYSIEEYYKMVAQVLDYKGAFVKD 265
+ I + V N+G+ + +Y + + L +
Sbjct: 229 RLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNML 288

Query: 266 LSKPVGMQQKLMDISK-QKALKWELEIPLEQGIKEAYEYYLKLLEV 310
+P + + D + + + E ++ G+K +Y +V
Sbjct: 289 PLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDFYKV 334


18HPAKL117_01220HPAKL117_01255N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_01220-2141.380699neutrophil-activating protein A
HPAKL117_01225-3121.187288histidine kinase sensor protein
HPAKL117_01230-2122.012636hypothetical protein
HPAKL117_01235-2122.518553flagellar basal body P-ring protein
HPAKL117_01240-1132.275684ATP-dependent RNA helicase
HPAKL117_01245-1111.892878hypothetical protein
HPAKL117_01250-2111.311675hypothetical protein
HPAKL117_01255-3112.176525oligopeptide permease ATPase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01220HELNAPAPROT1493e-49 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 149 bits (378), Expect = 3e-49
Identities = 39/140 (27%), Positives = 74/140 (52%), Gaps = 1/140 (0%)

Query: 5 EILKHLQADAIVLFMKVHNFHWNVKGTDFFNVHKATEEIYEGFADMFDDLAERIAQLGHH 64
L ++ +L+ K+H FHW VKG FF +H+ EE+Y+ A+ D +AER+ +G
Sbjct: 15 NSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIGGQ 74

Query: 65 PLVTLSEALKLTRVKEETKTSFHSKDIFKEILEDYKHLEKEFKELSNTAEKEGDKVTVTY 124
P+ T+ E + + + + + ++ + ++ DYK + E K + AE+ D T
Sbjct: 75 PVATVKEYTEHASITDGGNET-SASEMVQALVNDYKQISSESKFVIGLAEENQDNATADL 133

Query: 125 ADDQLAKLQKSIWMLQAHLA 144
+ +++K +WML ++L
Sbjct: 134 FVGLIEEVEKQVWMLSSYLG 153


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01225PF06580300.015 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.8 bits (67), Expect = 0.015
Identities = 10/71 (14%), Positives = 25/71 (35%), Gaps = 13/71 (18%)

Query: 281 IVLQNFLYNAIDAIEALEESEQ-GQVKIEAFIQNEFIVFTIIDNGKEVENKSALFEPFET 339
+++Q + N I + + Q G++ ++ N + + + G +
Sbjct: 258 MLVQTLVENGI--KHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK------- 308

Query: 340 TKLKGNGLGLA 350
+ G GL
Sbjct: 309 ---ESTGTGLQ 316


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01235FLGPRINGFLGI360e-126 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 360 bits (926), Expect = e-126
Identities = 119/345 (34%), Positives = 191/345 (55%), Gaps = 26/345 (7%)

Query: 19 AEKIGDIASVVGVRDNQLIGYGLVIGLNGTGDK-SGSKFTMQSISNMLESVNVKISADDI 77
+I DIAS+ RDNQLIGYGLV+GL GTGD S FT QS+ ML+++ +
Sbjct: 28 TSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQS 87

Query: 78 KSKNVAAVMITASLPPFARQGDKIDVQISSIGDAKSIQGGTLVMTPLNAVDGNIYALAQG 137
+KN+AAVM+TA+LPPFA G ++DV +SS+GDA S++GG L+MT L+ DG IYA+AQG
Sbjct: 88 NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQG 147

Query: 138 AITSGN-----------SNNLLSANIINGATIEREVSYDLFHKNAMVLSLKNPNFKNAIQ 186
A+ SA + NGA IERE+ +VL L+NP+F A++
Sbjct: 148 ALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPDFSTAVR 207

Query: 187 VQNTLNKV----FGNKVAIALDPKTIQITRPERFSMVEFLALVQEIPINYSAKNKIIVDE 242
V + +N +G+ +A D + I + +P + +A ++ + + K++++E
Sbjct: 208 VADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPAKVVINE 267

Query: 243 KSGTIVSGVDIMVHPIVVTSQDITLKITKEPLDN--------SKNAQDLDNNMSLDTAHN 294
++GTIV G D+ + + V+ +T+++T+ P Q + M++
Sbjct: 268 RTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGSK 327

Query: 295 TLSSNGKNITIAGVVKALQKIGVSAKGMVSILQALKKSGAISAEM 339
G ++ +V L IG+ A G+++ILQ +K +GA+ AE+
Sbjct: 328 VAIVEGPDLR--TLVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01240SECA300.028 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.028
Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 2/63 (3%)

Query: 261 IVFTRTKKEADELHQFLASKNYKSTALHGDMDQRDRRASIMAFKKNDADVLVATDVASRG 320
+V T + ++++ + L K L+ + A+I+A A V +AT++A RG
Sbjct: 453 LVGTISIEKSELVSNELTKAGIKHNVLNAKFHANE--AAIVAQAGYPAAVTIATNMAGRG 510

Query: 321 LDI 323
DI
Sbjct: 511 TDI 513


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01255HTHFIS320.006 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.7 bits (72), Expect = 0.006
Identities = 16/50 (32%), Positives = 21/50 (42%), Gaps = 7/50 (14%)

Query: 30 VAIVGESGSGKSSIANIIMRLNPR----FKPHNGEVLFETTNLLKESEAF 75
+ I GESG+GK +A + R F N + L ESE F
Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRD---LIESELF 209


19HPAKL117_01675HPAKL117_01745N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_01675-2121.430043hypothetical protein
HPAKL117_01680-2111.331119ribosomal large subunit pseudouridine synthase
HPAKL117_01685-1100.769857single-stranded-DNA-specific exonuclease
HPAKL117_01690-2101.390639CTP synthetase
HPAKL117_01695-3111.505788hypothetical protein
HPAKL117_01700-3101.020295flagellar MS-ring protein
HPAKL117_01705-4121.299863flagellar motor switch protein G
HPAKL117_01710-3121.398666flagellar assembly protein H
HPAKL117_01715-281.8702191-deoxy-D-xylulose-5-phosphate synthase
HPAKL117_017200111.244158GTP-binding protein LepA
HPAKL117_01725012-0.726474DNA-cytosine methyltransferase
HPAKL117_01730-1120.930277hypothetical protein
HPAKL117_017350120.591494flagellar basal-body rod protein
HPAKL117_017401110.185614alpha-ketoglutarate permease
HPAKL117_01745013-0.058565cell division protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01675PREPILNPTASE290.014 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 28.6 bits (64), Expect = 0.014
Identities = 18/59 (30%), Positives = 25/59 (42%), Gaps = 8/59 (13%)

Query: 32 FVIVAWLFRF--KSIAFSILITLLVILVDIWVYSDVRQFLL-DTASSPILLLAALLIKW 87
V VA ++A +L +LV L I D+ + LL D + P LL LL
Sbjct: 121 SVAVAMTLAPGWGTLAALLLTWVLVALTFI----DLDKMLLPDQLTLP-LLWGGLLFNL 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01700FLGMRINGFLIF5510.0 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 551 bits (1422), Expect = 0.0
Identities = 178/582 (30%), Positives = 292/582 (50%), Gaps = 66/582 (11%)

Query: 11 VDFFIKLNKKQKIALIAAGVLITALLVFLLLYPFKEKDYTQGGYGVLFERLDSSDNALIL 70
+++ +L +I LI AG A++V ++L+ K DY LF L D I+
Sbjct: 13 LEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWA-KTPDYR-----TLFSNLSDQDGGAIV 66

Query: 71 QHLQQNQIPYKILKDD-TILIPKDKVYEERITLASQGIPKTSKVGFEIFDTKDFGATDFD 129
L Q IPY+ I +P DKV+E R+ LA QG+PK VGFE+ D + FG + F
Sbjct: 67 AQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFS 126

Query: 130 QNIKLIRAIEGELSRTIESLNPILKANVHIAIPKDSVFVAKEVPPSASVMLKLKPDMKLS 189
+ + RA+EGEL+RTIE+L P+ A VH+A+PK S+FV ++ PSASV + L+P L
Sbjct: 127 EQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALD 186

Query: 190 PTQILGIKNLIAAAVPKLTTENVKIVNENGESIGEGDILENSKELALEQLHYKQNFENIL 249
QI + +L+++AV L NV +V+++G + + + + ++L QL + + E+ +
Sbjct: 187 EGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNT--SGRDLNDAQLKFANDVESRI 244

Query: 250 ENKIVNILAPIVGGKNKVVARVNAEFDFSQKKSTKETFDPNN-----VVRSEQNLEEKKE 304
+ +I IL+PIVG N V A+V A+ DF+ K+ T+E + PN +RS Q ++
Sbjct: 245 QRRIEAILSPIVGNGN-VHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQV 303

Query: 305 GASKKQVGGVPGVVSN-IGPVQGLKDNKEPEKYEKSQN---------------------- 341
GA GGVPG +SN P P + +QN
Sbjct: 304 GAGYP--GGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNE 361

Query: 342 TTNYEVGKTISEIKGEFGTLVRLNAAVVVDGKYKIALKDGANTLEYEPLSDESLKKINAL 401
T+NYEV +TI K G + RL+ AVVV+ K L DG + PL+ + +K+I L
Sbjct: 362 TSNYEVDRTIRHTKMNVGDIERLSVAVVVNYK---TLADG----KPLPLTADQMKQIEDL 414

Query: 402 VKQTIGYNQNRGDDVAVSNFEFNPMAPMLDNATLSEKIMHKTQKVLGSFTPLIKYILVFI 461
++ +G++ RGD + V N F+ + T E + Q + +++LV +
Sbjct: 415 TREAMGFSDKRGDTLNVVNSPFSAVDN-----TGGELPFWQQQSFIDQLLAAGRWLLVLV 469

Query: 462 VLFIFYKKVIVPFSERMLEVVPDEDKEVKSMFEEMDEEEDELNKLGDLRKKVEDQLGLNA 521
V +I ++K + P R +E ++ + E + E L+K L+++ +Q
Sbjct: 470 VAWILWRKAVRPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQ----- 524

Query: 522 TFSEEEVRYEIVLEKIRGTLKERPDEIAMLFKLLIKDEISSD 563
+ E++ ++IR E D + L+I+ +S+D
Sbjct: 525 -----RLGAEVMSQRIR----EMSDNDPRVVALVIRQWMSND 557


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01705FLGMOTORFLIG349e-122 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 349 bits (898), Expect = e-122
Identities = 121/338 (35%), Positives = 208/338 (61%), Gaps = 4/338 (1%)

Query: 8 KQKAQLDELSMSEKIAILLIQVGEDTTGEILRHLDIDSITEISKQIVQLNGTDKQIGAAV 67
K+ + L+ +K AILL+ +G + + ++ ++L + I ++ +I +L ++ V
Sbjct: 7 KEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNV 66

Query: 68 LEEFFAIFQSNQYINTGGLEYARELLTRTLGSEEARKVMDKLTKSLQTQKNFAYLGKIKP 127
L EF + + ++I GG++YARELL ++LG+++A +++ L +LQ+ + F ++ + P
Sbjct: 67 LLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQS-RPFEFVRRADP 125

Query: 128 QQLADFIINEHPQTIALILAHMEAPNAAETLSYFPDEMKAEISIRMANLGEISPQVVKRV 187
+ +FI EHPQTIALIL++++ A+ LS P E++ ++ R+A + SP+VV+ V
Sbjct: 126 ANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREV 185

Query: 188 STVLENKLESLTSYK-IEVGGLRAVAEIFNRLGQKSAKTTLARIESVDNKLAGAIKEMMF 246
VLE KL SL+S GG+ V EI N +K+ K + +E D +LA IK+ MF
Sbjct: 186 ERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMF 245

Query: 247 TFEDISKLDNFAIREILKVADKKDLSLALKTSTQDLTDKFLNNMSSRAAEQFVEEMQYLG 306
FEDI LD+ +I+ +L+ D ++L+ ALK+ + +K NMS RAA E+M++LG
Sbjct: 246 VFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLG 305

Query: 307 AVKIKDVDVAQRKIIEIVQSLQEKG--VIQTGEEEDVI 342
+ KDV+ +Q+KI+ +++ L+E+G VI G EEDV+
Sbjct: 306 PTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVL 343



Score = 31.3 bits (71), Expect = 0.006
Identities = 20/103 (19%), Positives = 41/103 (39%), Gaps = 3/103 (2%)

Query: 4 KLTPKQKAQLDELSMSEKIAILLIQVGEDTTGEILRHLDIDSITEISKQIVQLNGTDKQI 63
+ P + + IA++L + IL L + T ++++I ++ T ++
Sbjct: 122 RADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEV 181

Query: 64 GAA---VLEEFFAIFQSNQYINTGGLEYARELLTRTLGSEEAR 103
VLE+ A S Y + GG++ E++ E
Sbjct: 182 VREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKF 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01710FLGFLIH381e-05 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 38.2 bits (88), Expect = 1e-05
Identities = 47/212 (22%), Positives = 95/212 (44%), Gaps = 17/212 (8%)

Query: 45 PNPEEPLEKKAIENDLIDCLLKKTDELSSHLVKLQMQFEKAQEES-KALIENAKNDGYKI 103
P E + E +I+ + L L +LQMQ A E+ +A I + G+K
Sbjct: 17 PPQAEFVPIVEPEETIIE---EAEPSLEQQLAQLQMQ---AHEQGYQAGIAEGRQQGHKQ 70

Query: 104 GFKEGEEKMRNELTHSVNEEKNQLLHAITALDEKMKKSQDHLMALE----KELSAIAIDI 159
G++EG + L + E K+Q + + + + Q L AL+ L +A++
Sbjct: 71 GYQEG---LAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEA 127

Query: 160 AKEVILKEVEDNSQKVALALAEELLKNVLDATDIHLKVNPLDYPYLNERLQNASKI---K 216
A++VI + ++ + + + L + L + L+V+P D +++ L + +
Sbjct: 128 ARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGATLSLHGWR 187

Query: 217 LESNEAISKGGVMITSSNGSLDGNLMERFKTL 248
L + + GG +++ G LD ++ R++ L
Sbjct: 188 LRGDPTLHPGGCKVSADEGDLDASVATRWQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01720TCRTETOQM1425e-38 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 142 bits (359), Expect = 5e-38
Identities = 99/437 (22%), Positives = 174/437 (39%), Gaps = 85/437 (19%)

Query: 3 NIRNFSIIAHIDHGKSTLADCLIAECNAIS---NREMTSQVMDTMDIEKERGITIKAQSV 59
I N ++AH+D GK+TL + L+ AI+ + + + D +E++RGITI+
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 60 RLNYTLKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANVYIAL 119
+ E+ +N+IDTPGH+DF EV RSL +GA+L++ A GV+AQT +
Sbjct: 62 SFQW----ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 120 DNNLEILPVINKIDLPNANVLEVKQDIEDTIGIDCSGANEVSAKARLGIKD--------- 170
+ + INKID ++ V QDI++ + + +V + + +
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDT 177

Query: 171 -------LLEKIITTIPAPSGDFNAPLKALIYD-------------------------SW 198
LLEK ++ + + ++ +
Sbjct: 178 VIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNK 237

Query: 199 F--------------------DNYLGALALVRIMDGSINTEQEILVMGTGKKHGVLGLYY 238
F LA +R+ G ++ + + K + +Y
Sbjct: 238 FYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYT 296

Query: 239 PNPLKKIPTKSLECGEIGIV---SLGLKSVTDIAVGDTLTDAKNPTPKPIEGFMPAKPFV 295
+ GEI I+ L L SV +GDT P + IE P +
Sbjct: 297 SINGELCKIDKAYSGEIVILQNEFLKLNSV----LGDTKLL---PQRERIEN---PLPLL 346

Query: 296 FAGLYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERL 355
+ P + + E L +ALL++ +D L + +S+ + FLG + MEV L
Sbjct: 347 QTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATH---EIILSFLGKVQMEVTCALL 403

Query: 356 EREFGLNLIATAPTVVY 372
+ ++ + + PTV+Y
Sbjct: 404 QEKYHVEIEIKEPTVIY 420



Score = 31.0 bits (70), Expect = 0.015
Identities = 15/75 (20%), Positives = 28/75 (37%), Gaps = 2/75 (2%)

Query: 399 IKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDK 458
+ EP++ I P E+L + L + V+L+ +P+ I ++
Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCI-QEYRSD 592

Query: 459 LKSCTKGYASFDYEP 473
L T G + E
Sbjct: 593 LTFFTNGRSVCLTEL 607


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01735FLGHOOKAP1300.007 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 30.3 bits (68), Expect = 0.007
Identities = 9/40 (22%), Positives = 16/40 (40%)

Query: 3 NGYYAATGAMATQFNRLDLTSNNLANLNTNGFKRDDAITG 42
+ A + L+ SNN+++ N G+ R I
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMA 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01740TCRTETB417e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 41.0 bits (96), Expect = 7e-06
Identities = 58/315 (18%), Positives = 105/315 (33%), Gaps = 67/315 (21%)

Query: 37 APYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGDKKGRKTSMVYSIILMA 96
P A +F T + +AF++ G+ +GKL D+ G K +++ II+
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSI------GTAVYGKLSDQLGIKRLLLFGIIINC 90

Query: 97 LGSFMLALLPTKEIVGEWAFLFLLLARLLQGFSVGGE------YGVVATYLSELGKNGKK 150
GS + VG F L++AR +QG G VVA Y+ + +
Sbjct: 91 FGSVIGF-------VGHSFFSLLIMARFIQG--AGAAAFPALVMVVVARYIPKENRGKAF 141

Query: 151 GFYGSFQYVTLVGGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIM 210
G GS + +G + I I+ W YL + I + FL ++
Sbjct: 142 GLIGS---IVAMGEGVGPAIGGMIAHYIH---------WSYLLLIPMITIITVPFLMKLL 189

Query: 211 EETMDSGITSKTTIKEETQRGSLKELLNHKKALM-------IVFGLTMGGSLCFYTFTVY 263
+ + +K + K ++ + T +
Sbjct: 190 K-----------------KEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLS 232

Query: 264 LKIFLTNSSSFSPK-------ESSFIMLLALSYFILLQPLCG---MLADKIKRTQMLMVF 313
IF+ + + ++ M+ L I+ + G M+ +K L
Sbjct: 233 FLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA 292

Query: 314 AIAGLIVTPVVFYGI 328
I +I+ P I
Sbjct: 293 EIGSVIIFPGTMSVI 307


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_01745IGASERPTASE350.002 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 34.7 bits (79), Expect = 0.002
Identities = 47/242 (19%), Positives = 76/242 (31%), Gaps = 40/242 (16%)

Query: 169 FNPKKEGFENAPSDIQKKETKNDKEKENLKKNPIDENHKTPNEESFLAIPTPY------N 222
+NP+ E N D T N+ + + P + +E+ + P P
Sbjct: 981 YNPEVEK-RNQTVDTTNITTPNNIQADV-PSVPSNNEEIARVDEAPVPPPAPATPSETTE 1038

Query: 223 TTLNPLEPQEGLVQISSNPPTHYTI----YPKKNRFDDLSNPTNPTLKEIKQETKEREPT 278
T + + V+ + T T K+ + + +N + + ETKE + T
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTT 1098

Query: 279 PKKETLTL---------------------------ATPKPIMPASAPNTENDNKTENHKA 311
KET T+ + + P + P END T N K
Sbjct: 1099 ETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAREND-PTVNIKE 1157

Query: 312 PNHPTKEESPQENAQKEMIEENIKENIKEEEKEAQNAPSFSPITPTSAKKPVMVKELSEN 371
P T + E KE + + N+ +P T A V S N
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSN 1217

Query: 372 KE 373
K
Sbjct: 1218 KP 1219


20HPAKL117_02735HPAKL117_02765N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_02735214-1.165267hypothetical protein
HPAKL117_02740215-0.460167hypothetical protein
HPAKL117_02745116-0.267306dihydroorotase
HPAKL117_02750116-2.401455hypothetical protein
HPAKL117_02755-215-2.625297hypothetical protein
HPAKL117_02760-114-1.880937flagellar motor switch protein
HPAKL117_02765-113-0.619227endonuclease III
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02735TYPE3IMSPROT310.003 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 30.5 bits (69), Expect = 0.003
Identities = 19/64 (29%), Positives = 30/64 (46%), Gaps = 4/64 (6%)

Query: 87 LQSYSVMLFFNLLLLIDILGFLPFSIYHHFMASLIFSALFCSSLFLSSPLLGVIALVALS 146
L Y F L+L+ +LPFS S + + +L PLL V AL+A++
Sbjct: 45 LSDYYFEHFSKLMLIPAEQSYLPFSQ----ALSYVVDNVLLEFFYLCFPLLTVAALMAIA 100

Query: 147 SSLL 150
S ++
Sbjct: 101 SHVV 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02750TONBPROTEIN503e-09 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 49.6 bits (118), Expect = 3e-09
Identities = 24/57 (42%), Positives = 28/57 (49%)

Query: 83 APKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPNHKHKALKKVE 139
P P +P P P P P IEKPKP+PKPKPKP K + +K VE
Sbjct: 62 QPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVE 118



Score = 45.4 bits (107), Expect = 9e-08
Identities = 38/228 (16%), Positives = 69/228 (30%), Gaps = 53/228 (23%)

Query: 84 PKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPNHKHKALKKVEKVEE 143
P + P +P P P P P P E P KPKPKP+PK K V+KV+E
Sbjct: 57 PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKP--------KPVKKVQE 108

Query: 144 KKVVEEKKEEKKIVEQKVEQKKVEEKKPVKKEFDPNQLSFLPKEVAPPRQENNKGLDNQT 203
+ + K E + ++ + + +
Sbjct: 109 QPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQPQ------ 162

Query: 204 RRDIDELYGEEFGDLGTAEKDFIRNNLRDIGRITQKYLEYPQVAAYLGQDGTNAVEFYLH 263
YP A L +G V+F +
Sbjct: 163 ---------------------------------------YPARAQALRIEGQVKVKFDVT 183

Query: 264 PNGDISDLKIIIGSEYKMLDDNTLKTIQIAYKDYPRPKTKTLIRIRVR 311
P+G + +++I+ M + ++ + +P + ++ I +
Sbjct: 184 PDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFK 231



Score = 39.2 bits (91), Expect = 1e-05
Identities = 25/72 (34%), Positives = 31/72 (43%), Gaps = 1/72 (1%)

Query: 87 TLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPNHKHKALKKVEKVEEKKV 146
T+ P P PP P E P+PEP P+P E K K K + KKV
Sbjct: 48 TMVTPADLEPPQAVQPPPEPVVEPE-PEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKV 106

Query: 147 VEEKKEEKKIVE 158
E+ K + K VE
Sbjct: 107 QEQPKRDVKPVE 118



Score = 38.0 bits (88), Expect = 2e-05
Identities = 16/54 (29%), Positives = 23/54 (42%)

Query: 74 QDPSKNTQGAPKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKK 127
Q + +P P P P+ PKP KPKP+P K + +PK+
Sbjct: 59 QAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKR 112



Score = 33.8 bits (77), Expect = 6e-04
Identities = 14/56 (25%), Positives = 23/56 (41%)

Query: 74 QDPSKNTQGAPKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPKKPN 129
+P + P+P P++ P P P PKP K + +PK +P +
Sbjct: 65 PEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESR 120



Score = 30.3 bits (68), Expect = 0.009
Identities = 12/52 (23%), Positives = 16/52 (30%)

Query: 75 DPSKNTQGAPKPTLAGPQKPPTPPTPPIPPTPPKPIEKPKPEPKPKPKPEPK 126
+P P + P P P P K E+PK + KP
Sbjct: 72 EPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPAS 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02760FLGMOTORFLIN992e-30 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 99 bits (249), Expect = 2e-30
Identities = 25/77 (32%), Positives = 47/77 (61%)

Query: 34 LICDYKNLLDMEIVFSAELGSTQIPLLQILRFEKGSVIDLQKPAGESVDTFVNGRVIGKG 93
+ D ++D+ + + ELG T++ + ++LR +GSV+ L AGE +D +NG +I +G
Sbjct: 50 AMQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQG 109

Query: 94 EVMVFERNLAIRLNEIL 110
EV+V +R+ +I+
Sbjct: 110 EVVVVADKYGVRITDII 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02765OMS28PORIN280.020 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 28.2 bits (62), Expect = 0.020
Identities = 21/77 (27%), Positives = 42/77 (54%), Gaps = 8/77 (10%)

Query: 60 LFEKYPSVKDLAL-----ASLEEVKEIIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQ 114
+ K P+ K+L L A +E+VKE + + +++ + AQKV+ G+ PS +
Sbjct: 164 MLNKSPNNKELELTKEEFAKVEQVKETLMASERALDET---VQEAQKVLNMVNGLNPSNK 220

Query: 115 KELMSLEGVGQKTANVV 131
++++ + V + +NVV
Sbjct: 221 DQVLAKKDVAKAISNVV 237


21HPAKL117_02835HPAKL117_02905N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_02835-3100.425908methyl-accepting chemotaxis protein McpA
HPAKL117_02840-3120.791853flagellin A
HPAKL117_02845-4120.9809603-methyladenine DNA glycosylase
HPAKL117_02850-2121.311681hypothetical protein
HPAKL117_028551101.070133uroporphyrinogen decarboxylase
HPAKL117_02860190.628386outer-membrane protein of the hefABC efflux
HPAKL117_02865290.400041membrane fusion protein of the hefABC efflux
HPAKL117_02870190.314062cytoplasmic pump protein of the hefABC efflux
HPAKL117_02875111-0.389269hypothetical protein
HPAKL117_02880010-0.441914putative vacuolating cytotoxin (VacA)-like
HPAKL117_02885-212-1.638967hypothetical protein
HPAKL117_02890-310-0.489110ABC transporter ATP-binding protein
HPAKL117_02895-211-0.015702hypothetical protein
HPAKL117_02900-211-0.444606NAD-dependent DNA ligase LigA
HPAKL117_02905-212-0.415555chemotaxis protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02835OMS28PORIN290.042 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 28.6 bits (63), Expect = 0.042
Identities = 25/102 (24%), Positives = 48/102 (47%), Gaps = 2/102 (1%)

Query: 143 NAAKNGEEHSTEGLGTVNKTGQDIESLYEKMQNATSLADSLNQRS--NEITQVISLIDDI 200
N + ++ + L T+NK +D+ S E ++ ++ N + +SL+ D+
Sbjct: 47 NKKLDQKDQVNQALDTINKVTEDVSSKLEGVRESSLELVESNDAGVVKKFVGSMSLMSDV 106

Query: 201 AEQTNLLALNAAIEAARAGEHGRGFAVVADEVRKLAEKTQKA 242
A+ T + + A I A +G G V + +K ++TQKA
Sbjct: 107 AKGTVVASQEATIVAKCSGMVAEGANKVVEMSKKAVQETQKA 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02840FLAGELLIN2432e-76 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 243 bits (620), Expect = 2e-76
Identities = 125/518 (24%), Positives = 209/518 (40%), Gaps = 22/518 (4%)

Query: 2 AFQVNTNINAMNAHVQSALTQNALKTSLERLSSGLRINKAADDASGMTVADSLRSQASSL 61
A +NTN ++ +Q++L +++ERLSSGLRIN A DDA+G +A+ S L
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 GQAIANTNDGMGIIQVADKAMDEQLKILDTVKVKATQAAQDGQTTESRKAIQSDIVRLIQ 121
QA N NDG+ I Q + A++E L V+ + QA + K+IQ +I + ++
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 GLDNIGNTTTYNGQALLSGQFTNKEFQVGAYSNQSIKASIGSTTSDKIGQVRI-ATGALI 180
+D + N T +NG +LS + QVGA ++I + +G G
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDN-QMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKE 179

Query: 181 TASGDISLTFKQVDGVNDVTLESVKISSSAGTGIGVLAEVINKNSNQTGVKAYANVITTS 240
GD+ +FK V G + + + K +G V ++ V A +TT
Sbjct: 180 ATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTD 239

Query: 241 DVAVQSGSLSNLTLNGIHLGNIADIKKNDSDGRLVAAINAVTSETGVEAYTDQNGRLNLR 300
D N + K A A+ + + + +
Sbjct: 240 DAE-----------NNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTID 288

Query: 301 SLDGRGIEIKTDSVSDGPSALTMVNGGQDLTKGSTNYGRLSLTRLDAKSINV------VS 354
+ G K + +G V S + +N +
Sbjct: 289 TKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKT 348

Query: 355 ASDSQHLGFTAIGFGESQVAETTVNLRDVTGNFNANVKSASGANYNAVIASGNQSL---G 411
++S L ++ TVN + T N + + +G + S
Sbjct: 349 KNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINE 408

Query: 412 AGVTTLRGAMVVIDIAESAMKMLDKVRSDLGSVQNQMISTVNNISITQVNVKAAESQIRD 471
+ + +SA+ +D VRS LG++QN+ S + N+ T N+ +A S+I D
Sbjct: 409 DAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468

Query: 472 VDFAEESANFNKNNILAQSGSYAMSQANTVQQNILRLL 509
D+A E +N +K IL Q+G+ ++QAN V QN+L LL
Sbjct: 469 ADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02845PF05272310.004 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.004
Identities = 14/106 (13%), Positives = 26/106 (24%), Gaps = 20/106 (18%)

Query: 50 KSLEN-LKNAFILENDDEANLKKIAYIEFSKLAECVRPSGFYNQKAKRLIDLSENILKDF 108
L+ A +A+ E + VR + +KA
Sbjct: 446 WLLKPRRAALIEALRSAPALAGCVAFDELREQPVAVRAFPW--RKAPGP----------- 492

Query: 109 QSFENFKQEATREWLLDQKGIGKESADAILCYVCAKEVMVVDKYSY 154
E+ +++ G G+ SA + D
Sbjct: 493 --LEDADVLRLADYVETTYGTGEASAQTTEQAINV----AADMNRV 532


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02865RTXTOXIND511e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.0 bits (122), Expect = 1e-09
Identities = 22/69 (31%), Positives = 34/69 (49%)

Query: 40 STGIVDSIKVTEGSVVKKGDVLLLLYNQDKQAQSDSTEQQLIFAKKQYQRYSKIGGAVDK 99
IV I V EG V+KGDVLL L +A + T+ L+ A+ + RY + +++
Sbjct: 103 ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIEL 162

Query: 100 NTLESYEFN 108
N L +
Sbjct: 163 NKLPELKLP 171



Score = 31.3 bits (71), Expect = 0.003
Identities = 21/152 (13%), Positives = 48/152 (31%), Gaps = 25/152 (16%)

Query: 70 QAQSDSTEQQLIFAKKQYQR--YSKIGGAVDKNTLESYEFNYRRLESDYAYSIAVLNKTI 127
+++ S +++ + ++ K+ D + + E ++
Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTTDN--IGLLTLELAKNEER-------QQASV 329

Query: 128 LRAPFDGVIASKNIQVGEGVSANNTVLLRLVSHARKLVIE--FDSKYINAVKVG------ 179
+RAP + + GV L+ +V L + +K I + VG
Sbjct: 330 IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIK 389

Query: 180 -DTYTYSIDGDSNQHEAKITKIYP--TVDENT 208
+ + Y+ G K+ I D+
Sbjct: 390 VEAFPYTRYGYL---VGKVKNINLDAIEDQRL 418


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02870ACRIFLAVINRP8940.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 894 bits (2313), Expect = 0.0
Identities = 287/1040 (27%), Positives = 517/1040 (49%), Gaps = 42/1040 (4%)

Query: 1 MYKTAINRPITTLMFALAIVFFGTMGFKKLSVALFPKIDMPTVVVTTTYPGASAEIIESK 60
M I RPI + A+ ++ G + +L VA +P I P V V+ YPGA A+ ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTDKIEEAVMGIDGIKKVTSTSSKNVSIVV-IEFELEKPNEEALNDVVNKISSVR-FDDS 118
VT IE+ + GID + ++STS S+ + + F+ + A V NK+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 119 NIQKPSINKFDTDSQAIISLFVSSSSVPAT--TLNDYAKNTIKPMLQKISGVGGVQLNGF 176
+Q+ I+ + S ++ S + T ++DY + +K L +++GVG VQL G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 177 RERQIRIYADPTLMNKYNLTYADLFSTLKAENVEIDGGRIVNS------QRELSILINAN 230
+ +RI+ D L+NKY LT D+ + LK +N +I G++ + Q SI+
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 231 SYSVADVEKIQV-----GNHVRLGDIAKIEIGLEEDNTFASFKDKPGVILEIQKIAGANE 285
+ + K+ + G+ VRL D+A++E+G E N A KP L I+ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 286 IEIVDRVYEALKHIQAISP-NYEIRPFLDTTGYIRTSIEDVKFDLVLGAILAVLVVFAFL 344
++ + L +Q P ++ DTT +++ SI +V L +L LV++ FL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 345 RNGTITLVSAISIPISIMGTFALIQWMGFSLNMLTMVALTLAIGIIIDDAIVVIENIHK- 403
+N TL+ I++P+ ++GTFA++ G+S+N LTM + LAIG+++DDAIVV+EN+ +
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 404 KLEMGMNKRKASYEGVREIGFALVAISAMLLSVFVPIGNMKGIIGRFFQSFGITVALAIA 463
+E + ++A+ + + +I ALV I+ +L +VF+P+ G G ++ F IT+ A+A
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 464 LSYVVVVTIIPMVSSVVVNPRHS-------RFYVWSEPFFKALESYYTRLLQWVLNHKLI 516
LS +V + + P + + ++ P + F+ W F ++YT + +L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 517 IFIAVVLVFVGSLFVASKIGMEFMLKEDRGRFLVWLKAKPGVSIDY----MTQKSKIFQK 572
+ L+ G + + ++ F+ +ED+G FL ++ G + + + Q + + K
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 573 AIEKHAEVEFTTLQVGY-GTTQNPFKAKIFVQLKPLKERKKERKLGQFELMSALKKELRS 631
+ + E FT + G QN FV LKP +ER + ++ K EL
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNA--GMAFVSLKPWEERNGDENS-AEAVIHRAKMELGK 656

Query: 632 MPEAKGLESINLSEVSLIGGGGDSSPFQTFVFSHSQEAVDKSVANLKKFLLESPELKGKV 691
+ + + N+ + G ++ F + + D + L + + +
Sbjct: 657 IRDGF-VIPFNMPAIV---ELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASL 712

Query: 692 ESYHTSTSESQPQLQLKILRQNANKYGVSAQTIGSVVSSAFSGTSQASVFKQDGKEYDMI 751
S + E Q +L++ ++ A GVS I +S+A G + + F G+ +
Sbjct: 713 VSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGG-TYVNDFIDRGRVKKLY 771

Query: 752 IRVPDNKRVSVEDIKRLQVRNKYDKLMFLDALVEITETKSPSSISRYNRQRSVTVLAEPN 811
++ R+ ED+ +L VR+ +++ A + RYN S+ + E
Sbjct: 772 VQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEA- 830

Query: 812 RNAGVSLGEILTQVSKNTKEWLVEGANYRFTGEADNAKESNGEFLIALATAFVLIYMILA 871
G S G+ + + +N L G Y +TG + + S + +A +FV++++ LA
Sbjct: 831 -APGTSSGDAMALM-ENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLA 888

Query: 872 ALYESILEPFIIMVTMPLSFSGAFFALGLVHQPLSMFSMIGLILLIGMVGKNATLLIDVA 931
ALYES P +M+ +PL G A L +Q ++ M+GL+ IG+ KNA L+++ A
Sbjct: 889 ALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFA 948

Query: 932 NE-ERKKGLNIQEAILFAGKTRLRPILMTTIAMVCGMLPLALASGDGAAMKSPIGIAMSG 990
+ K+G + EA L A + RLRPILMT++A + G+LPLA+++G G+ ++ +GI + G
Sbjct: 949 KDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008

Query: 991 GLMISMVLSLLIVPVFYRLL 1010
G++ + +L++ VPVF+ ++
Sbjct: 1009 GMVSATLLAIFFVPVFFVVI 1028


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02880VACCYTOTOXIN2788e-78 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 278 bits (713), Expect = 8e-78
Identities = 104/394 (26%), Positives = 185/394 (46%), Gaps = 14/394 (3%)

Query: 2796 NAVNWLNALFVAKGGNPLFAPYYLQDTPTKHIVTLMEDVSSALGMLTKPSLKNNSTDVLQ 2855
+ L L + + +A + T I + ++ L + K + L
Sbjct: 907 QGRDLLQTLLI-DSHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLS 965

Query: 2856 LNTYTQQMGRLAKLSSFASFDSTDFSERLSSLKNQRFADAIPNAMDVILKYSQRDKLKNN 2915
L+ RL LS + F++RL +LK+QRFA + +A +V+ +++ + + N
Sbjct: 966 LSNAMILNSRLVNLSRRHTNHIDSFAKRLQALKDQRFAS-LESAAEVLYQFAPKYEKPTN 1024

Query: 2916 LWATGVGGISFVENGSGTLYGINLGYDRFVRG---VIVGGYAAYGYSGFNSR--ITGSRS 2970
+WA +GG S G+ +LYG + G D ++ G IVGG+ +YGYS F+++ S +
Sbjct: 1025 VWANAIGGTSLNSGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGA 1084

Query: 2971 DNVNVGLYARAFIKKSELTFSVNETWGANKTQISSNDALLSMINQSYNYNTWTTNARVNY 3030
+N N G+Y+R F + E F G++++ ++ ALL +NQSYNY ++ R +Y
Sbjct: 1085 NNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASY 1144

Query: 3031 GYDFMFKNKSVIIKPQISLGYYYIGMTGLDGVMNNALYNQFKANADPSKKSVLTINFAIE 3090
GYDF F ++++KP + + Y ++G T + S + + + +E
Sbjct: 1145 GYDFAFFRNALVLKPSVGVSYNHLGSTNFKS----NSNQKVALKNGASSQHLFNASANVE 1200

Query: 3091 NRHYFNKNSYFYAISGISRDLLVRSMGDKLVRFIGDNTLSYRKGELYNTFASITTGGEIR 3150
R+Y+ SYFY +G+ ++ + V + R NT A + GGE++
Sbjct: 1201 ARYYYGDTSYFYMNAGVLQEFANFGSSNA-VSLNTFKVNATRNP--LNTHARVMMGGELK 1257

Query: 3151 LFKSFYANAGVGARFGLDYKMINITGNIGMRLAF 3184
L K + N G L + + N+GMR +F
Sbjct: 1258 LAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF 1291



Score = 32.3 bits (73), Expect = 0.044
Identities = 19/99 (19%), Positives = 30/99 (30%), Gaps = 13/99 (13%)

Query: 661 YKFQGPKNAYTFKNTNFLA-GNFKFQGATTIEKSVLNDASYTF---DGVNN-AFNE---- 711
Y N N L G+ A I + + + G+N A E
Sbjct: 273 YSTINTSKVTGEVNFNHLTVGDHNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYK 332

Query: 712 ----DKFNGGSFNFNHAEQTNAFNNNSFNGGSFSFNANQ 746
DK + + N ++ + NNS N+ Q
Sbjct: 333 DKPNDKPSNTTQNNAKNDKQESSQNNSNTQVINPPNSAQ 371


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02895LCRVANTIGEN300.001 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 30.4 bits (68), Expect = 0.001
Identities = 16/33 (48%), Positives = 20/33 (60%)

Query: 16 KRKKLLIELAELEAEIKVSSERKSSFNISLSPS 48
R KL ELAEL AE+K+ S ++ N LS S
Sbjct: 149 ARSKLREELAELTAELKIYSVIQAEINKHLSSS 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_02905HTHFIS542e-10 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 54.5 bits (131), Expect = 2e-10
Identities = 24/110 (21%), Positives = 44/110 (40%), Gaps = 6/110 (5%)

Query: 194 ILIAEDSLSALKTLEKIVQTLELRYLAFPNGRELLDYLYEKEHYQQVGVVITDLEMPVIS 253
IL+A+D + L + + N L ++ +V+TD+ MP +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAA----GDGDLVVTDVVMPDEN 61

Query: 254 GFEVLKTIKADSRTEHLPVIINSSMSSDSNRQLAQSLEADGFVVKSNILE 303
F++L IK LPV++ S+ ++ A A ++ K L
Sbjct: 62 AFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


22HPAKL117_04250HPAKL117_04285N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_042500112.286648cysteinyl-tRNA synthetase
HPAKL117_042551112.733306vacuolating cytotoxin
HPAKL117_04260-1141.828960iron(III) dicitrate transporter, ATP-binding
HPAKL117_042650143.934286putative ABC transporter permease
HPAKL117_042700152.629062short-chain oxidoreductase
HPAKL117_042750173.043951hypothetical protein
HPAKL117_04280-1182.798450hypothetical protein
HPAKL117_042850172.778141hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04250OMS28PORIN300.019 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 29.8 bits (66), Expect = 0.019
Identities = 13/37 (35%), Positives = 25/37 (67%)

Query: 309 EEDLLVSKKRLDKIYRLKQRVLGTLGGINPNFKKEIL 345
+E L+ S++ LD+ + Q+VL + G+NP+ K ++L
Sbjct: 188 KETLMASERALDETVQEAQKVLNMVNGLNPSNKDQVL 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04255VACCYTOTOXIN18630.0 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 1863 bits (4827), Expect = 0.0
Identities = 1049/1333 (78%), Positives = 1137/1333 (85%), Gaps = 70/1333 (5%)

Query: 1 MELQQTHRKINRPIISLALVGALIGTELGANTPNDPIHSESRAFFTTVLIPAIVGGIATG 60
ME+QQTHRKINRP++SLALVGAL+ TP AFFTTV+IPAIVGGIATG
Sbjct: 1 MEIQQTHRKINRPLVSLALVGALVSI-----TP----QQSHAAFFTTVIIPAIVGGIATG 51

Query: 61 TAVGTVSGLLSWGLKQAEQANKTPDKPDKVWRIQAGRGFNNFPNKQYDLYKSLLSSKIDG 120
AVGTVSGLL WGLKQAE+ANKTPDKPDKVWRIQAG+GFN FPNK+YDLYKSLLSSKIDG
Sbjct: 52 AAVGTVSGLLGWGLKQAEEANKTPDKPDKVWRIQAGKGFNEFPNKEYDLYKSLLSSKIDG 111

Query: 121 GWDWGNAARHYWVKDGQWNKLEVGMQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFN 180
GWDWGNAARHYWVKDGQWNKLEV MQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFN
Sbjct: 112 GWDWGNAARHYWVKDGQWNKLEVDMQNAVGTYNLSGLINFTGGDLDVNMQKATLRLGQFN 171

Query: 181 GNSFTSFKDSADRTTRVNFDAKNILIDNFLEINNRVGSGAGRKASSTVLTLKSSEKITSR 240
GNSFTS+KDSADRTTRV+F+AKNILIDNFLEINNRVGSGAGRKASSTVLTL++SE ITSR
Sbjct: 172 GNSFTSYKDSADRTTRVDFNAKNILIDNFLEINNRVGSGAGRKASSTVLTLQASEGITSR 231

Query: 241 ENAEISLYDGATLNLVSSSNHSVDLYGKVWMGRLQYVGAYLAPSYSTINTSKVVGEMNFR 300
ENAEISLYDGATLNL S+S V L G VWMGRLQYVGAYLAPSYSTINTSKV GE+NF
Sbjct: 232 ENAEISLYDGATLNLASNS---VKLMGNVWMGRLQYVGAYLAPSYSTINTSKVTGEVNFN 288

Query: 301 HLAVGDQNAAQAGIIANKKTNIGVLDLWQSAGLSIIAPPEGGYESKTKD----------- 349
HL VGD NAAQAGIIA+ KT+IG LDLWQSAGL+IIAPPEGGY+ K D
Sbjct: 289 HLTVGDHNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPNDKPSNTTQNNAK 348

Query: 350 ----------------NPKNDTQKTEIQPTQVIDGPFAGAKDTVVNIFRLNTKSDGTLKA 393
NP N QKTEIQPTQVIDGPFAG K+TVVNI R+NT +DGT++
Sbjct: 349 NDKQESSQNNSNTQVINPPNSAQKTEIQPTQVIDGPFAGGKNTVVNINRINTNADGTIRV 408

Query: 394 GGFKASLSTNAAHLNIGEGGINLSNQASGRSLLVENLTGNITVEGALRVNNQVGGSAVAG 453
GGFKASL+TNAAHL+IG+GGINLSNQASGRSLLVENLTGNITV+G LRVNNQVGG A+AG
Sbjct: 409 GGFKASLTTNAAHLHIGKGGINLSNQASGRSLLVENLTGNITVDGPLRVNNQVGGYALAG 468

Query: 454 SSANFEFKAGEDTNNATATFNNDIHLGKAVNLRVDAHTAYFNGNIYLGKSTNLKVNGHSA 513
SSANFEFKAG DT N TATFNNDI LG+ VNL+VDAHTA
Sbjct: 469 SSANFEFKAGTDTKNGTATFNNDISLGRFVNLKVDAHTA--------------------- 507

Query: 514 HFKNIDASKSDNGLNTSALDLSGVTDKVNINKLTTAATNVSIKNFDIKELVVTTRVQSFG 573
+FK ID + G NT LD SGVT+KVNINKL TA+TNV++KNF+I ELVV T S G
Sbjct: 508 NFKGIDTG--NGGFNT--LDFSGVTNKVNINKLITASTNVAVKNFNINELVVKTNGVSVG 563

Query: 574 QYTIFGENIGDKSRIGVVSLQTGYSPAYSGGVTFKSGKKLVIDEIYHAPWNYFDARNVTD 633
+YT F E+IG +SRI V L+TG YSGGV FK G+KLVI++ Y+APWNYFDARN+ +
Sbjct: 564 EYTHFSEDIGSQSRINTVRLETGTRSIYSGGVKFKGGEKLVINDFYYAPWNYFDARNIKN 623

Query: 634 VEINKKILFGAPGYIAGKTGLMFNNLTLNSNASMDYGKDLDLTIQGHFTNNQGVMNLFVQ 693
VEI K+ FG G G LMFNNLTL NA MDY + +LTIQG F NNQG +N V+
Sbjct: 624 VEITNKLAFGPQGSPWGTAKLMFNNLTLGQNAVMDYSQFSNLTIQGDFVNNQGTINYLVR 683

Query: 694 DGRVATLNAGHQASMIFNNVVDSATGFYQPLIKINNAQNLTKNKEHVLVKARNIDYNLVG 753
G+VATLN G+ A+M F+N VDSATGFYQPL+KIN+AQ+L KNKEHVL+KA+ I Y G
Sbjct: 684 GGQVATLNVGNAAAMFFSNNVDSATGFYQPLMKINSAQDLIKNKEHVLLKAKIIGY---G 740

Query: 754 VQGASYDNISASNTNLMEQFKERLALYNNNNRMDICVVRNTDDIKACGMAIGNQAMVNNP 813
A D+I+ N NL+EQFKERLALYNNNNRMDICVVRNTDDIKACG AIGNQ+MVNNP
Sbjct: 741 NVSAGTDSIA--NVNLIEQFKERLALYNNNNRMDICVVRNTDDIKACGTAIGNQSMVNNP 798

Query: 814 DSYKYLIGKAWKNTGISKTANNTTIAVNLGKNSTPTENGGNTTDLPKNTTNKARFASYAL 873
++YKYL GKAWKN GISKTAN + I+V+ NSTPTENGGNTT+LP NTTNK RFASYAL
Sbjct: 799 ENYKYLEGKAWKNIGISKTANGSKISVHYLGNSTPTENGGNTTNLPTNTTNKVRFASYAL 858

Query: 874 IKNAPFAHYNATPNLVAINKHDFGTIESVFELANRSKDIDTLYASSGAQGRDLLQTLLID 933
IKNAPFA Y+ATPNLVAIN+HDFGTIESVFELANRS DIDTLYA+SGAQGRDLLQTLLID
Sbjct: 859 IKNAPFARYSATPNLVAINQHDFGTIESVFELANRSNDIDTLYANSGAQGRDLLQTLLID 918

Query: 934 SHNAGYARTMIDATSANEITKQLNEANSALNDIASLDHKTSSLQTLSLSNAMILNSRLVN 993
SH+AGYARTMIDATSANEITKQLN A + LN+IASL+HKTS LQTLSLSNAMILNSRLVN
Sbjct: 919 SHDAGYARTMIDATSANEITKQLNTATTTLNNIASLEHKTSGLQTLSLSNAMILNSRLVN 978

Query: 994 LSRRHTNNIDSFAKRLQALKGQRFASLESAAEVLYQFAPKYEKPTNVWANAIGGASLNNG 1053
LSRRHTN+IDSFAKRLQALK QRFASLESAAEVLYQFAPKYEKPTNVWANAIGG SLN+G
Sbjct: 979 LSRRHTNHIDSFAKRLQALKDQRFASLESAAEVLYQFAPKYEKPTNVWANAIGGTSLNSG 1038

Query: 1054 GNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFNNQANSLNSGANNTNFGVYSRIFAN 1113
GNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSF+NQANSLNSGANNTNFGVYSRIFAN
Sbjct: 1039 GNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFSNQANSLNSGANNTNFGVYSRIFAN 1098

Query: 1114 QHEFDFEAQGAIGSDQSSLNFKSALLQDLNQSYNYLAYSAAARASYGYDFAFFRNALVLK 1173
QHEFDFEAQGA+GSDQSSLNFKSALL+DLNQSYNYLAYSAA RASYGYDFAFFRNALVLK
Sbjct: 1099 QHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASYGYDFAFFRNALVLK 1158

Query: 1174 PSVGVSYNHLGSTNFESNS-QLVALKNGASSQHLFNASANVEARYYYGDTSYFYMNAGVL 1232
PSVGVSYNHLGSTNF+SNS Q VALKNGASSQHLFNASANVEARYYYGDTSYFYMNAGVL
Sbjct: 1159 PSVGVSYNHLGSTNFKSNSNQKVALKNGASSQHLFNASANVEARYYYGDTSYFYMNAGVL 1218

Query: 1233 QEFANFGSSNAVSLNTFRVNVARNPLNTHARVMMGGELQLAKEVFLNLGFIYAHNLISNA 1292
QEFANFGSSNAVSLNTF+VN RNPLNTHARVMMGGEL+LAKEVFLNLGF+Y HNLISN
Sbjct: 1219 QEFANFGSSNAVSLNTFKVNATRNPLNTHARVMMGGELKLAKEVFLNLGFVYLHNLISNI 1278

Query: 1293 GHFASNLGMRYSF 1305
GHFASNLGMRYSF
Sbjct: 1279 GHFASNLGMRYSF 1291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04270DHBDHDRGNASE865e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 86.3 bits (213), Expect = 5e-22
Identities = 59/243 (24%), Positives = 108/243 (44%), Gaps = 12/243 (4%)

Query: 1 MSEKKESQKVAIITGASSGIGLECALMLLDQGYKVYALSRHATLCVALNNALC------E 54
M+ K K+A ITGA+ GIG A L QG + A+ + + ++L E
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60

Query: 55 SIDVDVSDSNALKEAFLNISAKEDHCDVLINSAGYGVFGSVEDTPIDEVKKQFGVNFFAL 114
+ DV DS A+ E I + D+L+N AG G + +E + F VN +
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 115 CEVVQFCLPLLKNKPYSKIFNLSSIAGRVSMLFLGHYSASKHALEAYSDALRLELKPFNI 174
+ + ++ I + S V + Y++SK A ++ L LEL +NI
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 175 QVCLIEPGPVKSNWEKTAFSVESFESENSLYALEVNAAKSFYSGV-YQKALSPKAVAQKI 233
+ ++ PG +++ + + ++ EN + + ++F +G+ +K P +A +
Sbjct: 181 RCNIVSPGSTETDMQWSLWA-----DENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAV 235

Query: 234 VFL 236
+FL
Sbjct: 236 LFL 238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_04285SECA290.003 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.5 bits (66), Expect = 0.003
Identities = 13/69 (18%), Positives = 33/69 (47%), Gaps = 12/69 (17%)

Query: 4 SPTKKDYTQYSEKQLFNLINQLEQKIKKMQNDRASFKEKMAKELEKRDQNFKDKIDALNE 63
S + + +++ N+IN +E +++K+ ++ EL+ + F+ +++
Sbjct: 12 SRNDRTLRRM--RKVVNIINAMEPEMEKLSDE----------ELKGKTAEFRARLEKGEV 59

Query: 64 LLQKISQAF 72
L I +AF
Sbjct: 60 LENLIPEAF 68


23HPAKL117_07655HPAKL117_07690N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
HPAKL117_07655-2122.596869flagellar hook-basal body protein FliE
HPAKL117_07660-1122.459754flagellar basal body rod protein FlgC
HPAKL117_076651122.050810flagellar basal body rod protein FlgB
HPAKL117_076702121.624968cell division protein FtsW
HPAKL117_076751130.534183iron(III) ABC transporter, periplasmic
HPAKL117_076802140.508426putative peroxidase
HPAKL117_07685113-0.069706outer membrane protein
HPAKL117_07690114-0.130074penicillin-binding protein 2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07655FLGHOOKFLIE776e-22 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 77.0 bits (189), Expect = 6e-22
Identities = 19/77 (24%), Positives = 40/77 (51%), Gaps = 1/77 (1%)

Query: 34 EQKGGEFSKLLKQSINELNNTQEQSDKALADMATGQIK-DLHQAAIAIGKAETSMKLMLE 92
Q F+ L +++ +++TQ + G+ L+ + KA SM++ ++
Sbjct: 27 PQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQ 86

Query: 93 VRNKAISAYKELLRTQI 109
VRNK ++AY+E++ Q+
Sbjct: 87 VRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07660FLGHOOKAP1290.011 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 28.8 bits (64), Expect = 0.011
Identities = 10/38 (26%), Positives = 15/38 (39%)

Query: 121 NVNAVVEMADLVEATRAYQANVAAFQSAKNMAQNAIGM 158
VN E +L + Y AN Q+A + I +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07675FERRIBNDNGPP361e-04 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 36.1 bits (83), Expect = 1e-04
Identities = 30/183 (16%), Positives = 78/183 (42%), Gaps = 10/183 (5%)

Query: 108 NVELLKKLSPDLVVTFVG-NPKAVEHAKKFGISFLSFQETT--IAEAMQAMQ--AQAAVL 162
N+ELL ++ P +V G P A+ +F + +A A +++ A L
Sbjct: 88 NLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNL 147

Query: 163 EIDASKKFAKMQETLDFIKERL-KDVKKKKGVELFHKAN--KISGHQAISSDILEKGGID 219
+ A A+ ++ + +K R K + + + G ++ +IL++ GI
Sbjct: 148 QSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIP 207

Query: 220 N-FGLKYVKFGRADVSVEKIVK-ENPEIIFIWWVSPLTPEDVLNNPKFSTIKAIKNKQVY 277
N + + +G VS++++ ++ +++ + + ++ P + + ++ +
Sbjct: 208 NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQ 267

Query: 278 KLP 280
++P
Sbjct: 268 RVP 270


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
HPAKL117_07690TYPE3IMPPROT290.029 Type III secretion system inner membrane P protein ...
		>TYPE3IMPPROT#Type III secretion system inner membrane P protein

family signature.
Length = 224

Score = 29.4 bits (66), Expect = 0.029
Identities = 9/23 (39%), Positives = 12/23 (52%)

Query: 4 LRYKLLLFVFIGFWGLLVLNLFI 26
KL+LFV + W LL L +
Sbjct: 195 TPIKLVLFVALDGWTLLSKGLIL 217



 
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