:: PredictBias ::

A) Input parameters
Genome	NC_013853.gbk	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in NC_013853 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

smi_2089

smi_2062

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_2089	1	26	-7.401874	chromosome partitioning protein
smi_2088	1	25	-7.741181	serine protease
smi_2087	1	28	-6.878174	23S rRNA
smi_2085	1	26	-7.612612	*competence stimulating peptide (CSP) precursor
smi_2084	1	22	-6.596533	histidine kinase
smi_2083	0	21	-5.693325	response regulator
smi_2080	-1	22	-4.451299	**hypothetical protein
smi_2079	-1	21	-4.542491	hypothetical protein
smi_2078	-1	19	-4.425593	hypothetical protein
smi_2077	-1	19	-2.997563	ABC-F family ATPase
smi_2076	-2	23	-3.996426	tryptophanyl-tRNA synthetase
smi_2075	-1	23	-4.253430	inosine monophosphate dehydrogenase
smi_2074	-2	26	-5.581987	recombination protein recF
smi_2073	-3	25	-4.662048	hypothetical protein
smi_2072	-3	26	-3.998724	zinc-dependent protease
smi_2071	-4	26	-3.439908	zinc-dependent protease
smi_2070	-3	25	-2.719896	hypothetical protein
smi_2069	-2	26	-3.213465	phosphatidylglycerophosphate synthase
smi_2068	0	25	-2.846390	ABCtransporter ATP-binding protein cobalt
smi_2067	2	22	-2.500861	cobalt ABC transporter ATP-binding protein
smi_2066	5	24	-2.363636	ABC transporter permease, cobalt transport
smi_2065	6	29	-1.754955	cell shape determining protein MreC
smi_2064	6	30	-1.299065	cell shape determining protein MreD
smi_2063	5	25	-0.544395	general stress protein GSP-781 amidase
smi_2062	2	20	-0.577264	30S ribosomal protein S2

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2088	V8PROTEASE	62	1e-12	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 61.6 bits (149), Expect = 1e-12 Identities = 31/165 (18%), Positives = 58/165 (35%), Gaps = 34/165 (20%) Query: 117 IVTNNHVINGASKVDIRLS------------DGTKVPGEIVGADTFSDIAVVKISSEKVT 164 ++TN HV++ L +G +I D+A+VK S + Sbjct: 114 LLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQN 173 Query: 165 -------TVAEFGDSSKLTVGETAIAIGSPLG-SEYANTVTQGIVSSLNRNVSLKSEDGQ 216 A ++++ V + G P ++G ++ Sbjct: 174 KHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITY------------- 220 Query: 217 AISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSV 261 + +A+Q D + GNSG P+ N + +VIGI + +V Sbjct: 221 -LKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAV 264
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2087	56KDTSANTIGN	27	0.037	Rickettsia 56kDa type-specific antigen protein sign...
>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein signature. Length = 533 Score = 27.2 bits (60), Expect = 0.037 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%) Query: 14 KYLKDGIAEYSKRISRFAKLEMIELADEKTPDRASESENQ-KILEI 58 K L D I + I FA + I + D P+ AS + Q KI E+ Sbjct: 262 KVLSDKIIQIYSDIKPFADIAGINVPDTGLPNSASIEQIQSKIQEL 307
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2080	HTHTETR	49	1e-09	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 49.2 bits (117), Expect = 1e-09 Identities = 22/104 (21%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 6 KRLKTKRTIENAMVQLLMEQPFDQISTVKLAEKAGISRSSFYTHYKDKYDMIEHYQSKLF 65 + +T++ I + ++L +Q S ++A+ AG++R + Y H+KDK D+ Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67 Query: 66 HTF-EYIFQKHAHHK-------RDAILEVFEYLESEPLLAALLS 101 E + A R+ ++ V E +E L+ Sbjct: 68 SNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLME 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2079	ICENUCLEATIN	34	0.003	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 34.0 bits (77), Expect = 0.003 Identities = 59/181 (32%), Positives = 74/181 (40%), Gaps = 33/181 (18%) Query: 479 STSLTGLSSGLTEIQGTLTSKLVPASQSITSGVNAY-TAGVDK---VSQGASQLSEKNST 534 ST G S LT G+ ++ S ++T+G + TAG D G+S S S Sbjct: 982 STQTAGYQSTLTAGYGS--TQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSF 1039 Query: 535 LTGSLDQLVSGSTTLTQKSSKLTAGVGQLVEKTPELVSGIEKLST---GSNQLNQKSQEL 591 LT GST ++ S LTAG G L+SG T GSNQ+ L Sbjct: 1040 LTAGY-----GSTLISGLRSVLTAGYGS------SLISGRRSSLTAGYGSNQIASHRSSL 1088 Query: 592 IAGVDKLQ-----------SGSSQLADKSSQLISGAS--QLESGANKLADGAGKLAEGGT 638 IAG + Q GSSQ A S LISGA Q+ KL GA G Sbjct: 1089 IAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGADSVQMAGERGKLIAGADSTQTAGD 1148 Query: 639 K 639 + Sbjct: 1149 R 1149
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2077	PF05272	32	0.009	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 31.6 bits (71), Expect = 0.009 Identities = 11/30 (36%), Positives = 14/30 (46%) Query: 32 LIGANGAGKSTFLKILAGDIEPTTGHISLG 61 L G G GKST + L G + H +G Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIG 630
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2063	GPOSANCHOR	51	5e-09	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 50.8 bits (121), Expect = 5e-09 Identities = 40/230 (17%), Positives = 76/230 (33%), Gaps = 9/230 (3%) Query: 27 AETTDDKIAAQDNKISNLTAQQQEAQKQVDQVQEQVSAIQTEQSNLQSENDRLQAESKKL 86 D ++ + +KI L A++ + +K ++ +A + L++E L A L Sbjct: 101 LRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADL 160 Query: 87 EGEITELSKNIVSRNDSLQ-----KQARSAQTNGAATNYINTIVNSKSITEAISRVAAMS 141 E + + + ++ K A A+ + S + + I + A Sbjct: 161 EKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEK 220 Query: 142 EIVSANNKMLEQQKADKKAISEKQVANNDAINTVIA----NQQKLADDAQSLTTKQAELK 197 ++A LE+ S A + A Q +L + Sbjct: 221 AALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADS 280 Query: 198 AAELNLAAEKATAEDEKASLLEKKAAAEAEAKAAAEAEAAYKAKQASQQQ 247 A L AEKA E EKA L + A ++ A + + + Sbjct: 281 AKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEA 330 Score = 33.9 bits (77), Expect = 0.001 Identities = 24/199 (12%), Positives = 67/199 (33%), Gaps = 5/199 (2%) Query: 31 DDKIAAQDNKISNLTAQQQEAQKQVDQVQEQVSAIQTEQSNLQSENDRLQAESKKLEGEI 90 + + A + + ++ ++ + +A+ +++L+ + S +I Sbjct: 189 EARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKI 248 Query: 91 TELSKNIVSRNDSLQKQARSAQTNGAATNYINTIVNSKSITEAISRVAAMSEIVSANNKM 150 L + + ++ + + + I + AA+ + Sbjct: 249 KTLEAEKAALEARQAELEKALEGAMNFSTADS-----AKIKTLEAEKAALEAEKADLEHQ 303 Query: 151 LEQQKADKKAISEKQVANNDAINTVIANQQKLADDAQSLTTKQAELKAAELNLAAEKATA 210 + A+++++ A+ +A + A QKL + + + L+ K Sbjct: 304 SQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL 363 Query: 211 EDEKASLLEKKAAAEAEAK 229 E E L E+ +EA + Sbjct: 364 EAEHQKLEEQNKISEASRQ 382 Score = 31.6 bits (71), Expect = 0.006 Identities = 40/219 (18%), Positives = 93/219 (42%), Gaps = 18/219 (8%) Query: 28 ETTDDKIAAQDNKISNLTAQQQEAQKQVDQVQEQVSAIQTEQSNLQSENDRLQAESKKLE 87 +T + + AA + + + L + A ++ ++ E++ L++E L+ +S+ L Sbjct: 249 KTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLN 308 Query: 88 GEITELSKNIVSRNDSLQKQARSAQTNGAATNYINTIVNSKSITEAISRVAAMSEIVSAN 147 L +++ + ++ ++ Q + I+EA SR + ++ ++ Sbjct: 309 ANRQSLRRDLDASREAKKQLEAEHQK----------LEEQNKISEA-SRQSLRRDLDASR 357 Query: 148 NKMLEQQKADKKAISEKQVANNDAINTVIANQQKLADDAQSLTTKQAELKAAELNLAA-E 206 + + +K + +++ + ++ L ++ + L+ A LAA E Sbjct: 358 EAKKQLEAEHQKLEEQNKISEASRQSL----RRDLDASREAKKQVEKALEEANSKLAALE 413 Query: 207 KATAEDE--KASLLEKKAAAEAEAKAAAEAEAAYKAKQA 243 K E E K ++KA +A+ +A A+A AKQA Sbjct: 414 KLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQA 452

smi_2023

smi_1999

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_2023	-1	19	3.883916	ABC zinc transporter, metal-binding lipoprotein
smi_2022	0	21	3.750263	5'-nucleotidase
smi_2021	1	19	2.208754	hypothetical protein
smi_2020	2	19	0.894402	beta-galactosidase/beta-glucuronidase,
smi_2019	2	19	0.187820	hypothetical protein
smi_2018	2	20	-0.760186	fucosidase
smi_2017	1	23	-4.738319	sugar hydrolase
smi_2016	1	25	-4.948848	phage-related integrase, recombinase
smi_2015	0	30	-2.335535	transcriptional regulator
smi_2014	0	33	-1.538167	transcriptional regulator
smi_2013	0	35	-0.510626	transcriptional regulator
smi_2012	1	36	-1.574555	hypothetical protein
smi_2011	3	40	1.160970	hypothetical protein
smi_2010	5	34	0.207017	hypothetical protein
smi_2009	4	32	-0.170821	hypothetical protein
smi_2008	4	30	0.460313	hypothetical protein
smi_2007	4	27	-0.116118	hypothetical protein
smi_2006	4	26	-0.456731	hypothetical protein
smi_2005	3	26	-1.234779	replication protein
smi_2004	-1	22	1.336338	phage-related DNA primase
smi_2003	-1	17	2.581933	hypothetical protein
smi_2002	-2	17	3.255289	hypothetical protein
smi_2001	-1	20	4.375784	hypothetical protein
smi_2000	-1	22	4.132716	hypothetical protein
smi_1999	-2	21	3.882675	transcriptional regulator

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2001	PF07520	26	0.022	Virulence protein SrfB
>PF07520#Virulence protein SrfB Length = 1041 Score = 25.7 bits (56), Expect = 0.022 Identities = 10/37 (27%), Positives = 19/37 (51%), Gaps = 5/37 (13%) Query: 9 IEARGEGDSPHISLWFDKQLRDVFISLISLSFCKQLR 45 +EA +G++ + LW L+++F L F + R Sbjct: 195 LEADEDGNAVDLQLWVSDWLKEMF-----LDFKRAER 226

smi_1989

smi_1948

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1989	3	20	1.528992	hypothetical protein
smi_1988	2	19	1.788097	hypothetical protein
smi_1987	-1	18	2.273785	hypothetical protein
smi_1986	-1	19	2.996632	hypothetical protein
smi_1985	-1	20	3.664141	hypothetical protein
smi_1984	-1	20	3.560401	transcriptional regulator
smi_1983	-1	15	3.030792	cyanate permease
smi_1982	-2	17	2.279071	glycosyl transferase
smi_1981	-1	19	2.022990	nucleoside-diphosphate sugar isomerase
smi_1980	0	20	-1.078808	phosphatase
smi_1979	1	23	-5.331153	L-serine dehydratase, alpha subunit
smi_1978	2	29	-7.181608	L-serine dehydratase, beta subunit
smi_1977	3	32	-8.472029	LysM domain protein
smi_1976	1	27	-7.217743	transcriptional regulator
smi_1975	1	26	-6.725331	hypothetical protein
smi_1974	1	21	-5.013221	hypothetical protein
smi_1973	0	18	-2.799903	hypothetical protein
smi_1972	-1	17	-1.427660	ABC transporter ATP-binding protein
smi_1971	-1	16	-1.112825	hypothetical protein
smi_1970	-1	17	-0.918945	hypothetical protein
smi_1969	-1	15	0.397008	ABC transporter ATP-binding protein
smi_1968	-3	14	1.996409	ABC transporter substrate-binding protein
smi_1967	-2	17	2.357403	argininosuccinate synthase
smi_1966	-1	21	4.013185	argininosuccinate lyase
smi_1965	0	23	4.718173	hypothetical protein
smi_1964	0	25	5.296387	hypothetical protein
smi_1963	0	22	4.874741	transposase, ISSmi1
smi_1962	-1	22	3.680596	tRNA methyl transferase
smi_1961	0	23	3.412692	hypothetical protein
smi_1960	-1	24	1.412294	NAD/FAD-binding enzyme GidA
smi_1959	1	25	0.909829	hydrolase
smi_1958	-2	25	1.745105	hypothetical protein
smi_1957	0	25	2.769628	hypothetical protein
smi_1956	0	25	4.945641	cibC
smi_1955	1	23	3.886840	excreted peptide
smi_1954	-1	22	3.094094	excreted peptide
smi_1953	1	20	3.083492	transposase, ISSmi1
smi_1952	0	20	2.654015	molecular chaperone
smi_1951	0	14	1.797807	acetyltransferase
smi_1950	1	13	-0.206031	metal-dependent protease
smi_1949	4	25	0.213897	transcriptional regulator
smi_1948	2	20	2.126485	metal-dependent CAAX amino terminal membrane

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1988	IGASERPTASE	42	4e-06	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 42.0 bits (98), Expect = 4e-06 Identities = 29/165 (17%), Positives = 58/165 (35%), Gaps = 14/165 (8%) Query: 7 ESNDFVKTSSKNKPDEQAQDGADKAEETIPDLDTPIEKNTQLEKEVSQAEAELESQQEEK 66 ++ + K + N + ++ + T K T ++ +A+ E E QE Sbjct: 1064 QNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVP 1123 Query: 67 IETPEDS--EAKTETEEKKALDSTEEEPDLSKETEKVTKAEENQEALSQQKTTTKEPLLL 124 T + S + ++ET + +A + E +P +E Q + T + Sbjct: 1124 KVTSQVSPKQEQSETVQPQAEPARENDP--------TVNIKEPQSQTNTTADTEQPAKET 1175 Query: 125 SKSLESPYIPDQAQKSTDRWKEQVLDFWSWLVEALKSPTSKLETS 169 S ++E P + + E + A PT E+S Sbjct: 1176 SSNVEQPVTESTTVNTGNSVVENPEN----TTPATTQPTVNSESS 1216 Score = 36.6 bits (84), Expect = 2e-04 Identities = 26/133 (19%), Positives = 51/133 (38%), Gaps = 2/133 (1%) Query: 9 NDFVKTSSKNKP-DEQAQDGADKAE-ETIPDLDTPIEKNTQLEKEVSQAEAELESQQEEK 66 N V T++ P + QA + + E I +D E E+ ++E Sbjct: 989 NQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQES 1048 Query: 67 IETPEDSEAKTETEEKKALDSTEEEPDLSKETEKVTKAEENQEALSQQKTTTKEPLLLSK 126 ++ + TET + + E + ++ T+ A+ E Q T TKE + K Sbjct: 1049 KTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEK 1108 Query: 127 SLESPYIPDQAQK 139 ++ ++ Q+ Sbjct: 1109 EEKAKVETEKTQE 1121 Score = 33.5 bits (76), Expect = 0.002 Identities = 32/159 (20%), Positives = 57/159 (35%), Gaps = 21/159 (13%) Query: 20 PDEQAQDGADKAEETIPDLDTPIEKNTQ--LEKEVSQAEAELESQQEEKIETPEDSEAKT 77 P E + E +EKN Q E E E++ K T + A++ Sbjct: 1033 PSETTE----TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQS 1088 Query: 78 ETEEKKALDSTEEEPDLSKETEKVTKAEENQEALSQQKTTTKEPLLLSKSLESPYIPDQA 137 +E K+ + +KET V K EE + +++ T + P + S+ + Sbjct: 1089 GSETKET------QTTETKETATVEK-EEKAKVETEK--TQEVPKVTSQVSPKQEQSETV 1139 Query: 138 QKSTDRWKEQVLDFWSWLVEALKSPTSKLETSSTHSYTA 176 Q + +E +K P S+ T++ A Sbjct: 1140 QPQAEPAREND------PTVNIKEPQSQTNTTADTEQPA 1172
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1986	TCRTETB	28	0.025	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 27.9 bits (62), Expect = 0.025 Identities = 19/103 (18%), Positives = 36/103 (34%), Gaps = 4/103 (3%) Query: 76 NRQILHIALLAL---LAAPIGIPLGIAILVSL-FAILVAALTVILAFFAVSILGIIGGFL 131 N +L+++L + P + L F+I A + + L + G + Sbjct: 29 NEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIII 88 Query: 132 FLVESFTVLAQAKSAFILIFGSGLLAIGASSLVLLGISYVARF 174 S +LI + GA++ L + VAR+ Sbjct: 89 NCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARY 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1984	TCRTETA	44	5e-07	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 44.4 bits (105), Expect = 5e-07 Identities = 64/360 (17%), Positives = 118/360 (32%), Gaps = 17/360 (4%) Query: 6 LFFVPGIILIGVSLRTPFTVLPIILGDISQGLGVEVSSLGVLTSLPLLMFTLFSLFSTRL 65 + + +G+ L P VLP +L D+ + G+L +L LM + L Sbjct: 10 ILSTVALDAVGIGLIMP--VLPGLLRDLVHS-NDVTAHYGILLALYALMQFACAPVLGAL 66 Query: 66 AQKIGLEHFFTYSLFFLTIGSLIRLI--NLPLLYLGTL---MVGASIAVINVLLPSLIQA 120 + + G SL + I L +LY+G + + GA+ AV + + Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDG 126 Query: 121 NQ-PKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGLILLLTLLCLATFLVWLP-- 177 ++ + GF++ + M L + A + L FL+ Sbjct: 127 DERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHK 186 Query: 178 -NHRYNHRLAPQTKQKSQTKVMHNKQVWAVIVFAGFQSLLFYTAMTWLPTMAIHAGLSSH 236 R R A + + VF Q + A W+ + Sbjct: 187 GERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDAT 246 Query: 237 EAGLLTSIFSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGMVGISMLFFPVGNFFYW 296 G+ + F ++ I + R LML + +A G +L F W Sbjct: 247 TIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGM--IADGTGYILLAFATR---GW 301 Query: 297 LAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSFDL 356 +A +++ A+ + + + E+ QL G + + GP LF + Sbjct: 302 MAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAA 361
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1983	BCTERIALGSPF	38	5e-05	Bacterial general secretion pathway protein F signa...
>BCTERIALGSPF#Bacterial general secretion pathway protein F signature. Length = 408 Score = 37.5 bits (87), Expect = 5e-05 Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 10/85 (11%) Query: 186 DLLWLNMIATGAKTGNLDQILCQVRVGAGMFERRGGLRYLKLYRQARQRMLKRGQISYME 245 + L+ M+A G +G+LD +L ++ A E+R +R + +Q M+ Sbjct: 132 ERLYCAMVAAGETSGHLDAVLNRL---ADYTEQRQQMR-----SRIQQAMIY--PCVLTV 181 Query: 246 YAKSVAIQMVVALCPGFVRQFIFMK 270 A +V ++ + P V QFI MK Sbjct: 182 VAIAVVSILLSVVVPKVVEQFIHMK 206
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1982	NUCEPIMERASE	81	9e-19	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 81.0 bits (200), Expect = 9e-19 Identities = 50/284 (17%), Positives = 91/284 (32%), Gaps = 57/284 (20%) Query: 294 TILVTGAGGSIGSEICRQ----------VSRFNPERIVLLGHGENSIYLVYHELIRKFQG 343 LVTGA G IG + ++ + N V L EL+ + Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQAR-------LELLAQ--- 51 Query: 344 IDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAR 403 + D+ D + + +F V+ + V NP +N+ G N+ Sbjct: 52 PGFQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILE 111 Query: 404 AVDEAKVPKMVMIST---------------DKAVNPPNVMGATKRVAELIVTGFNQRSQS 448 K+ ++ S+ D +P ++ ATK+ EL+ ++ Sbjct: 112 GCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL 171 Query: 449 TYCAVRFGNVLGSRGS---VIPVFERQIAEGGPVTV-TDFRMTRYFMTI----------- 493 +RF V G G + F + + EG + V +M R F I Sbjct: 172 PATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQ 231 Query: 494 -------PEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKM 530 + + A V+ + PV++ D + + Sbjct: 232 DVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQAL 275
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1978	PF03544	30	0.004	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 30.3 bits (68), Expect = 0.004 Identities = 19/64 (29%), Positives = 24/64 (37%), Gaps = 3/64 (4%) Query: 66 VHMIYVGQELVIDGPAAPVAPASTTYEAPAAQDE--AVSATVAETIEVEEETPAASGTVA 123 ++Y VI+ PA P P S T APA + AV +E E E Sbjct: 30 AGLLYTSVHQVIELPA-PAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPK 88 Query: 124 EETV 127 E V Sbjct: 89 EAPV 92
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1959	BACINVASINB	25	0.036	Salmonella/Shigella invasin protein B signature.
>BACINVASINB#Salmonella/Shigella invasin protein B signature. Length = 593 Score = 25.1 bits (54), Expect = 0.036 Identities = 12/43 (27%), Positives = 23/43 (53%) Query: 31 SELEGRIAARQLVEENRPEYNIEYIELLSNKLLDYEKETGAFE 73 S+LE R+A Q + E++ E I+ + L + ++ T +E Sbjct: 102 SQLESRLAVWQAMIESQKEMGIQVSKEFQTALGEAQEATDLYE 144
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1952	SACTRNSFRASE	31	0.001	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 31.1 bits (70), Expect = 0.001 Identities = 22/75 (29%), Positives = 34/75 (45%), Gaps = 7/75 (9%) Query: 48 LAYDGTEVIGFLAVQENIFE-AEVLQIAVKGAYQGKGIASAL------FAQLSTDKEIFL 100 L Y IG + ++ N A + IAV Y+ KG+ +AL +A+ + + L Sbjct: 69 LYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLML 128 Query: 101 EVRKSNQRAQAFYKK 115 E + N A FY K Sbjct: 129 ETQDINISACHFYAK 143

smi_1935

smi_1929

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1935	0	27	3.687606	hypothetical protein
smi_1934	0	27	4.394234	NrdI family protein
smi_1933	0	26	3.796113	hypothetical protein
smi_1932	0	22	3.949044	transcriptional regulator
smi_1931	-1	23	3.675898	hypothetical protein
smi_1930	-1	22	3.859950	DNA mismatch repair protein hexB
smi_1929	0	21	3.917429	holiday junction DNA helicase RuvA

smi_1870

smi_1847

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1870	2	20	-0.446760	leucyl-tRNA synthetase
smi_1869	1	21	-0.559854	ABC transporter ATP-binding protein
smi_1868	0	22	0.026253	ABC transporter permease
smi_1867	1	21	1.971492	transcriptional regulator
smi_1866	1	22	0.864457	hypothetical protein
smi_1863	2	27	0.885174	acetyltransferase
smi_1862	1	27	2.389119	acetyltransferase
smi_1861	0	24	2.720502	transposase, ISSmi3
smi_1860	-1	21	2.368507	hypothetical protein
smi_1859	0	19	1.665815	hypothetical protein
smi_1857	-2	15	2.617859	holliday junction DNA helicase RuvB
smi_1856	-2	14	3.027769	hypothetical protein
smi_1855	-1	17	3.039246	transposase, ISSmi3
smi_1854	-1	15	3.242181	transposase, ISSmi1
smi_1853	-2	15	3.189829	UDP diphosphate synthase
smi_1852	0	22	3.467002	phosphatidate cytidylyltransferase
smi_1851	1	26	3.904113	metallo protease
smi_1850	2	28	3.920771	prolyl-tRNA synthetase
smi_1849	1	26	3.300477	glycosyl hydrolase
smi_1848	2	28	2.928953	glutamine--fructose-6-phosphate transaminase
smi_1847	4	30	2.979686	oxidoreductase

smi_1806

smi_1775

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1806	0	18	3.059690	rRNA methylase
smi_1805	0	18	3.032441	FMN riboswitch (RFN element)
smi_1804	0	16	3.071918	hypothetical protein
smi_1803	0	16	3.385421	phosphatase
smi_1802	0	15	2.778375	hypothetical protein
smi_1801	0	18	3.211196	hypothetical protein
smi_1800	-1	14	0.111257	T-box leader
smi_1799	-1	16	-0.718524	phenylalanyl-tRNA synthetase, alpha chain
smi_1798	1	22	-2.951108	hypothetical protein
smi_1797	1	30	-6.808120	phenylalanyl-tRNA synthetase, beta chain
smi_1796	0	30	-7.997393	Na+ ABC transporter ATP-binding protein
smi_1795	1	33	-9.348456	ABC transporter permease, Na+ export
smi_1794	2	37	-10.159646	transposase, ISSmi1
smi_1793	2	37	-10.148300	50S ribosomal protein L13
smi_1792	2	35	-10.169653	30S ribosomal protein S9
smi_1791	1	33	-9.813166	integrase
smi_1790	1	34	-9.554792	hypothetical protein
smi_1789	1	32	-9.582399	hypothetical protein
smi_1788	0	28	-8.246200	serine/threonine metallophosphatase
smi_1787	1	27	-8.191826	Ser/Thr protein phosphatase
smi_1786	0	30	-7.310126	hypothetical protein
smi_1785.1	2	30	-7.298191	FtsK/SpoIIIE family protein
smi_1785	1	28	-7.220958	hypothetical protein
smi_1784	3	22	-5.100402	hypothetical protein
smi_1783	2	19	-2.782786	hypothetical protein
smi_1782	2	19	-0.572307	hypothetical protein
smi_1781	3	19	-0.381251	hypothetical protein
smi_1780	2	20	-0.373276	drug transporter, major facilitator superfamily
smi_1779	2	22	1.515985	transcriptional regulator
smi_1778	3	26	2.141485	integrase/recombinase, phage integrase family,
smi_1777	3	24	0.738007	integrase/recombinase, phage integrase family,
smi_1776	3	20	-1.543398	ATPase
smi_1775	3	23	-1.425953	oligopeptide permease

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1803	GPOSANCHOR	29	0.033	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 29.3 bits (65), Expect = 0.033 Identities = 19/89 (21%), Positives = 35/89 (39%), Gaps = 8/89 (8%) Query: 32 EEQLKALREETLASLKQIT-AENEKEMQDLRVSVLGKKGSLTEI--LKGMKDVSAEMRPI 88 E + L + Q+ A + +DL S KK E L+ +S R Sbjct: 294 EAEKADLEHQ-----SQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQS 348 Query: 89 IGKHVNEARDVLTAAFEETAKLLEEKKVA 117 + + ++ +R+ E KL E+ K++ Sbjct: 349 LRRDLDASREAKKQLEAEHQKLEEQNKIS 377
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1794	PF08280	27	0.013	M protein trans-acting positive regulator
>PF08280#M protein trans-acting positive regulator Length = 530 Score = 26.7 bits (59), Expect = 0.013 Identities = 8/49 (16%), Positives = 21/49 (42%), Gaps = 2/49 (4%) Query: 15 SMITNLLQNEISHFKE--DLGLDSPYLNKGQTCKYLGISNNTLDGWIQK 61 S+I L++ I + L + L + + G++ L+ + ++ Sbjct: 33 SLIEKYLESSIESKCQLVVLFFKTSSLPITEVAEKTGLTFLQLNHYCEE 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1784	TCRTETB	113	2e-29	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 113 bits (283), Expect = 2e-29 Identities = 95/409 (23%), Positives = 177/409 (43%), Gaps = 22/409 (5%) Query: 12 LLFILSLGYIMAVLDTTGVVLAVPHIETAMSVSLEQSIWIINAYTLALGSLLLLSGNLTT 71 +L L + +VL+ + +++P I + + W+ A+ L + G L+ Sbjct: 15 ILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD 74 Query: 72 KYGAKRILLIGMTIFTLASLGCSFSPNIETLIIL-RFIQGFGTSLFMPSSLALLFISYPD 130 + G KR+LL G+ I S+ + +L+I+ RFIQG G + F P+ + ++ Y Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAF-PALVMVVVARYIP 133 Query: 131 STKRARMLGIWTAIISVATGTGSFIGGLIINYFGWRGIFLVNIPFGILTVISILLLVK-- 188 R + G+ +I+++ G G IGG+I +Y W +L+ IP ++T+I++ L+K Sbjct: 134 KENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIP--MITIITVPFLMKLL 189 Query: 189 NNIANLKTRIDIFSNIFLVTTIGSLVIYLVEGNQYGYSNSNLLLFLLLFILFAIGLVVHD 248 +K DI I + I ++ ++ S + FL++ +L + V H Sbjct: 190 KKEVRIKGHFDIKGIILMSVGIVFFML---------FTTSYSISFLIVSVLSFLIFVKHI 240 Query: 249 KKSKTPIVPHQLLKNAKFIISNLLGLVVNISLYGIVLVLGLYFQTYLNLSSMVSG-LLIL 307 +K P V L KN F+I L G ++ ++ G V ++ + LS+ G ++I Sbjct: 241 RKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIF 300 Query: 308 PGMIVLIIGNLFYARAVKRFSVGSLATVSIIFAIVGAAGIFGIGVLFHEIQLYILIPLFS 367 PG + +II V R + + + F V L ++ I + Sbjct: 301 PGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV---SFLTASFLLETTSWFMTIIIVF 357 Query: 368 LMSLGIGVLTPATTTILMEAAGQELSGIAGATLNANKQIGGLFGTTIMG 416 ++ T +T + QE +G + LN + G I+G Sbjct: 358 VLGGLSFTKTVISTIVSSSLKQQE-AGAGMSLLNFTSFLSEGTGIAIVG 405

smi_1749

smi_1693

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1749	-1	19	3.225657	hypothetical protein
smi_1748	-1	20	2.589239	DNA methylase
smi_1747	0	23	2.846766	hypothetical protein
smi_1746	0	24	2.795198	6-phosphogluconate dehydrogenase,
smi_1745	1	23	2.252566	response regulator
smi_1744	1	16	1.843439	choline binding protein CbpF
smi_1743	-1	20	2.331833	mevalonate kinase
smi_1742	-1	21	2.824403	mevalonate pyrophosphate decarboxylase
smi_1741	-1	23	3.081643	phosphomevalonate kinase
smi_1740	0	23	2.515333	isopentenyl-diphosphate:dimethylallyl
smi_1739	-1	23	2.693162	hypothetical protein
smi_1738	-1	23	3.834116	histidine kinase
smi_1737	0	22	2.458974	response regulator
smi_1736	0	19	1.167561	DNA alkylation repair enzyme, truncation
smi_1735	-2	15	-0.520821	FKBP-type peptidyl-prolyl cis-trans isomerase
smi_1734	-1	17	-2.072519	ATP-dependent exoDNAse (exonuclease V), alpha
smi_1733	0	18	-2.982784	signal peptidase I
smi_1732	1	28	-6.644192	ribonuclease HIII
smi_1731	-1	34	-7.695244	hypothetical protein
smi_1730	0	29	-6.311857	hypothetical protein
smi_1729	2	25	-6.621971	mismatch repair ATPase (MutS family)
smi_1728	3	21	-4.500714	transcriptional regulator
smi_1727	3	16	-2.396231	hypothetical protein
smi_1726	3	14	-1.432721	HSP70 family protein
smi_1725	2	12	0.205923	dnaJ domain protein
smi_1724	2	13	-0.766823	grpE domain protein
smi_1723	1	15	-0.718017	hypothetical protein
smi_1722	4	20	-3.582772	hypothetical protein
smi_1721	4	25	-6.104479	choline binding domain Cbp6
smi_1720	5	28	-6.708890	choline binding protein cbp12
smi_1719	4	27	-6.385581	hypothetical protein
smi_1718	5	26	-5.618107	Na+/alanine symporter
smi_1717	5	25	-5.031705	hypothetical protein
smi_1716	5	27	-2.488440	exfoliative toxin A
smi_1714	6	27	-2.347161	TetR family transcriptional regulator
smi_1713	6	28	-1.634419	ABC transporter ABC nucleotide-binding domain
smi_1712	9	32	-2.204298	hypothetical protein
smi_1711	8	33	-1.394015	site specific recombinase, authentic frame
smi_1710	9	34	-2.582102	hypothetical protein
smi_1709	8	35	-7.375020	site-specific recombinase
smi_1708	7	37	-7.844115	nucleotidyltransferase
smi_1707	9	40	-9.824502	methyltransferase
smi_1706	7	42	-11.190171	streptomycin aminoglycoside 6-adenyltransferase
smi_1705	6	45	-11.834782	streptothricin acetyltransferase
smi_1704	4	44	-12.161064	aminoglycoside 3'-phosphotransferase
smi_1703	4	30	-7.544278	hypothetical protein
smi_1702	4	34	-8.904986	hypothetical protein
smi_1701	2	31	-7.738366	acetyl transferase
smi_1700	4	27	-5.358287	aminoglycoside acetyltransferase and
smi_1699	4	26	-3.936117	hypothetical protein
smi_1698	3	25	-3.216219	hypothetical protein
smi_1697	4	25	-1.622462	hypothetical protein
smi_1696	3	26	0.377914	hypothetical protein
smi_1695	2	25	-0.137701	hypothetical protein
smi_1694	3	24	-1.431943	multidrug ABC transporter ATPase/permease
smi_1693	-1	25	-3.288356	AraC family transcriptional regulator

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1749	HTHFIS	51	1e-09	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 50.6 bits (121), Expect = 1e-09 Identities = 21/111 (18%), Positives = 45/111 (40%) Query: 4 RILLLEKERNLAHFLSLELQKEQYRVDQVEEGQKALSMALQTDYDLILLNARLGDMTAQD 63 IL+ + + + L+ L + Y V D DL++ + + D A D Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 64 FADKLSRTKPASVIMVLDHREELQGQIETIQRFAVSYIYKPVIIENLVARI 114 ++ + +P ++V+ + I+ ++ A Y+ KP + L+ I Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGII 115
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1742	PF06580	36	2e-04	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 36.0 bits (83), Expect = 2e-04 Identities = 18/91 (19%), Positives = 43/91 (47%), Gaps = 9/91 (9%) Query: 244 ILQELISNTLRHA-----QASCLDVYLYQTDVELQLKVVDNGIGFQLGSFDELSYGLRNI 298 ++Q L+ N ++H Q + + + + + L+V + G + + GL+N+ Sbjct: 259 LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQNV 318 Query: 299 KERVEDMAG---TVQLLTAPKQGLAVDIRIP 326 +ER++ + G ++L + A+ + IP Sbjct: 319 RERLQMLYGTEAQIKLSEKQGKVNAM-VLIP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1741	HTHFIS	66	4e-15	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 66.4 bits (162), Expect = 4e-15 Identities = 31/161 (19%), Positives = 61/161 (37%), Gaps = 14/161 (8%) Query: 2 KILLVDDHEMVRLGLKSYFDLQD-DVEVVGEAANGSQGIDLALELRPDVIVMDIVMPEMN 60 IL+ DD +R L DV + N + D++V D+VMP+ N Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITS---NAATLWRWIAAGDGDLVVTDVVMPDEN 61 Query: 61 GIDATLAILKEWPEAKILIVTSYLDNEKIMPVLNAGAKGYMLKTSSADELLHAVRKVAAG 120 D I K P+ +L++++ + GA Y+ K EL+ + + Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR---- 117 Query: 121 KLAIEQEVSKKVEYHRNHIELHEDLTARE---RDVLQLIAK 158 E ++ + + L R +++ +++A+ Sbjct: 118 ---ALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLAR 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1730	SHAPEPROTEIN	129	9e-36	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 129 bits (327), Expect = 9e-36 Identities = 82/364 (22%), Positives = 142/364 (39%), Gaps = 51/364 (14%) Query: 2 SKAIGIDLGTTYSAVSVLSETGQPQILLNQDGENLTPSVVFFQDFDGKDEP----LVGIQ 57 S + IDLGT + + V + I+LN+ PSVV D P VG Sbjct: 10 SNDLSIDLGTANTLIYVKGQG----IVLNE------PSVVAI-RQDRAGSPKSVAAVGHD 58 Query: 58 AKNLAASSPEAVVQYVKRQMGNPNWKFDSPSDTVYTAEEISAIILKRLKEGAENALGDKV 117 AK + +P + R M D + E++ +K++ N+ Sbjct: 59 AKQMLGRTPGNIA--AIRPMK------DGVIADFFVTEKMLQHFIKQVHS---NSFMRPS 107 Query: 118 EDVVITVPAYFDDARRTATKHAGEIAGLNVLRVLNEPTAAALAYGISAEKNETVLVYDLG 177 V++ VP R A + + + AG + ++ EP AAA+ G+ + +V D+G Sbjct: 108 PRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIG 167 Query: 178 GGTFDVTLMKIKDGEFDVIATDGDRNLGGFDFDNALSMIIAEKME----EQGAEDIYTDE 233 GGT +V ++ + + +GG FD A+ + E AE I E Sbjct: 168 GGTTEVAVI-----SLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKH-E 221 Query: 234 HFTALLREKSENTK-RGLTTVEKTNVFLDYKGKSYKIPITRVEFEEATKSLMNRTEELLD 292 +A ++ + RG E G + E EA + + + Sbjct: 222 IGSAYPGDEVREIEVRGRNLAE---------GVPRGFTLNSNEILEALQEPLTGIVSAVM 272 Query: 293 DVVEESG--MSWDEIDQ-VLLIGGSTRMPMVQRKLEEKIGKKIVYSINPDEAVAQGAAIQ 349 +E+ ++ D ++ ++L GG + + R L E+ G +V + +P VA+G Sbjct: 273 VALEQCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGK- 331 Query: 350 AALE 353 ALE Sbjct: 332 -ALE 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1727	60KDINNERMP	30	0.011	60kDa inner membrane protein signature.
>60KDINNERMP#60kDa inner membrane protein signature. Length = 548 Score = 29.9 bits (67), Expect = 0.011 Identities = 13/57 (22%), Positives = 23/57 (40%), Gaps = 9/57 (15%) Query: 11 RRNILYFILGFL-------WGRRQNAKISPEPPTLSTPK--HSELPSISKATHNGKM 58 +RN+L L F+ W + +N + + T +T S A+ GK+ Sbjct: 4 QRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKL 60
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1722	ACRIFLAVINRP	31	0.014	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 30.6 bits (69), Expect = 0.014 Identities = 37/262 (14%), Positives = 85/262 (32%), Gaps = 56/262 (21%) Query: 161 TFTQLNAITESIQNTTTISPAITALVLSVLVAIAVFGGLKSISKISTAVVPFMAI-IYIL 219 + + SI + A++L LV +++ ++P +A+ + +L Sbjct: 326 PYDTTPFVQLSIH--EVVKTLFEAIMLVFLVMYLFLQNMRA------TLIPTIAVPVVLL 377 Query: 220 GTLTVIFFNVGKLPATIALILTSAFSPVAAVGGFAGASIRMAIQNGVARGVFSNESGLGS 279 GT ++ ++ + F V A+G +I + ++N V R + + Sbjct: 378 GTFAILAAF------GYSINTLTMFGMVLAIGLLVDDAIVV-VEN-VERVMMED----KL 425 Query: 280 APIAAAAAKTNEPVEQGLISMTGTFIDTLI-ICTLTGLTILVTGVWSGDLNGVALTQSAF 338 P A ++ ++ L+ + I + G T G Q + Sbjct: 426 PPKEATEKSMSQ-IQGALVGIAMVLSAVFIPMAFFGGST------------GAIYRQFSI 472 Query: 339 STVFSHFGPALLTIFLVLFAFTTIL------------GWNYYGERCFEFLFG-------- 378 + V + L+ + L T+L G+ + F+ Sbjct: 473 TIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGK 532 Query: 379 -VRFIWLYRVVFVLMVLLGGFI 399 + Y +++ L+V + Sbjct: 533 ILGSTGRYLLIYALIVAGMVVL 554
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1719	HTHTETR	70	3e-17	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 70.4 bits (172), Expect = 3e-17 Identities = 29/161 (18%), Positives = 63/161 (39%), Gaps = 5/161 (3%) Query: 10 RRAEIMDAAMILFMEKGYTNTTTQDIVDKVNISRGLLYYHFKNKEDILYCLVEQYSDRLL 69 R I+D A+ LF ++G ++T+ +I ++RG +Y+HFK+K D+ + E + Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71 Query: 70 KDIYIIAYDEDKTAIEKIRS----FIDVTIISSENISAEGTVLQKTIDLKENQYMIDKLS 125 + + +R ++ T+ + K + E ++ + Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMA-VVQQAQ 130 Query: 126 HKLVEKLTIYFEKILNQGIMERVFSVKYPLETAELLMTAYV 166 L + E+ L I ++ A ++M Y+ Sbjct: 131 RNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYI 171
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1714	PF03627	28	0.013	PapG
>PF03627#PapG Length = 336 Score = 28.0 bits (62), Expect = 0.013 Identities = 16/73 (21%), Positives = 28/73 (38%), Gaps = 11/73 (15%) Query: 74 DEETFEKAR-----EEKRKRAEKLG----RIREPKDEPKTDYPVKFKAKPLVQKYEDPYK 124 DE F+ E + EK ++ P D P DY V +Q++ Y Sbjct: 128 DERAFDAGNLCQKPGETTRLTEKFDDIIFKVALPADLPLGDYSVTIPYTSGMQRHFASYL 187 Query: 125 QAEY--AYSLIES 135 A + Y++ ++ Sbjct: 188 GARFKIPYNVAKT 200
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1709	SACTRNSFRASE	289	e-103	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 289 bits (740), Expect = e-103 Identities = 87/180 (48%), Positives = 124/180 (68%), Gaps = 7/180 (3%) Query: 1 MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEAPYLKSYQDEEDEEDEE 60 MI +M ++KD +KP+EPF V G++IP +EN WT+TE + PY K Y+D++ + Sbjct: 1 MIMKMTHLNMKDFNKPNEPFVVFGRMIPAFENGVWTYTEERFSKPYFKQYEDDDMD---- 56 Query: 61 ADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDIAVCKDFRGQGIGSALINI 120 + Y++ K +LYY ++ C+G++K+R NWN YA IEDIAV KD+R +G+G+AL++ Sbjct: 57 ---VSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHK 113 Query: 121 SIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDTMLYANFENNFEKAVFWYLRF 180 +IEWAK + GLMLETQD N+ AC FY F IG+VDTMLY+NF E A+FWY +F Sbjct: 114 AIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAVDTMLYSNFPTANEIAIFWYYKF 173
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1705	SACTRNSFRASE	44	4e-08	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 44.2 bits (104), Expect = 4e-08 Identities = 25/98 (25%), Positives = 39/98 (39%), Gaps = 4/98 (4%) Query: 59 DIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKTMFYLHSIGMLPNYQDKGYG 118 D D ++ + F+ N IG +R + + I + +Y+ KG G Sbjct: 52 DDDMDVSYVEEEGKAAFLYYLENNCIG----RIKIRSNWNGYALIEDIAVAKDYRKKGVG 107 Query: 119 SKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLG 156 + LL E++KE + L T N ACH Y K Sbjct: 108 TALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHH 145
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1704	SACTRNSFRASE	34	8e-04	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 33.8 bits (77), Expect = 8e-04 Identities = 15/51 (29%), Positives = 23/51 (45%), Gaps = 1/51 (1%) Query: 105 PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRI 155 +Y KG+GT + E+ KE + ++L+ N A Y K F I Sbjct: 99 KDYRKKGVGTALLHKAIEW-AKENHFCGLMLETQDINISACHFYAKHHFII 148

smi_1682

smi_1653

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1682	2	16	2.773313	aspartate kinase
smi_1681	1	16	2.829929	enoyl-CoA hydratase/carnithine racemase
smi_1680	1	16	2.709516	transcription regulator, MarR family
smi_1679	0	16	3.201981	3-oxoacyl-ACP synthase III
smi_1678	-1	17	3.443406	acyl carrier protein
smi_1677	-2	16	1.849852	enoyl-ACP reductase II
smi_1676	-2	16	1.417677	ACP S-malonyltransferase
smi_1675	-2	16	0.626585	3-oxoacyl-ACP reductase
smi_1674	0	23	-0.369699	3-oxoacyl-ACP synthase
smi_1673	1	25	-2.303923	acetyl-CoA carboxylase
smi_1672	-1	26	0.294190	3-hydroxymyristoyl/3-hydroxydecanoyl-ACP
smi_1671	0	32	2.832906	acetyl-CoA carboxylase biotin carboxylase
smi_1670	0	33	3.095890	acetyl-CoA carboxylase subunit beta
smi_1669	0	30	2.700789	acetyl-CoA carboxylase carboxyl transferase
smi_1668	-2	26	3.669119	excreted peptide
smi_1667	3	16	4.324376	metal-dependent CAAX amino terminal membrane
smi_1666	2	11	2.137293	transcription termination factor nusB
smi_1665	2	14	1.143569	hypothetical protein
smi_1664	1	15	0.549571	translation elongation factor P
smi_1663	0	16	-0.461728	hypothetical protein
smi_1662	1	19	-1.822378	glutamyl tRNA-Gln amidotransferase chain B
smi_1661	1	23	-6.119770	glutamyl-tRNA(Gln) amidotransferase, A subunit
smi_1660	1	27	-6.269558	glutamyl tRNA-Gln amidotransferase C subunit
smi_1659	1	27	-6.626119	hypothetical protein
smi_1658	2	26	-6.519852	peptide chain release factor 3
smi_1657	2	27	-6.608404	cell wall surface anchor family protein, Ser
smi_1656	2	27	-5.669813	glycosyl transferase
smi_1655	3	27	-5.351194	hypothetical protein
smi_1654	2	21	-3.200447	hypothetical protein
smi_1653	2	20	-2.323959	glycosyl transferase family 8

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1680	DHBDHDRGNASE	124	9e-37	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 124 bits (312), Expect = 9e-37 Identities = 79/254 (31%), Positives = 131/254 (51%), Gaps = 13/254 (5%) Query: 3 LENKNIFITGSSRGIGLAIAHKFAQAGANIV-LNSRGAISEELLAEFSNYGVKVVPISGD 61 +E K FITG+++GIG A+A A GA+I ++ E++++ D Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 62 VSDFADAKRMVEQAIAELGSIDVLVNNAGITQDTLMLKMTEADFEKVLKVNLTGAFNMTQ 121 V D A + + E+G ID+LVN AG+ + L+ +++ ++E VN TG FN ++ Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125 Query: 122 SVLKPMIKAREGAIINMSSVVGLMGNIGQANYAASKAGLIGFTKSVAREVANRNIRVNAI 181 SV K M+ R G+I+ + S + A YA+SKA + FTK + E+A NIR N + Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185 Query: 182 APGMIESDMTAVL------SDKVKDAMLAQ----IPMKEFGQAEQVADLTVFLAGQD--Y 229 +PG E+DM L +++V L IP+K+ + +AD +FL + Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245 Query: 230 LTGQVVAIDGGLSM 243 +T + +DGG ++ Sbjct: 246 ITMHNLCVDGGATL 259
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1664	CARBMTKINASE	28	0.003	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 28.3 bits (63), Expect = 0.003 Identities = 9/28 (32%), Positives = 17/28 (60%), Gaps = 1/28 (3%) Query: 7 VTNLEVTKDD-IYKNPSNPILRMYDDDE 33 +T V K+D ++NP+ P+ YD++ Sbjct: 114 ITQTIVDKNDPAFQNPTKPVGPFYDEET 141
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1663	TCRTETOQM	232	4e-71	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 232 bits (593), Expect = 4e-71 Identities = 108/451 (23%), Positives = 206/451 (45%), Gaps = 41/451 (9%) Query: 9 KRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGTFAKSDWMDIEKQRGISVT 68 K +++H DAGKTT+TE LLY G I E G+V GT ++D +E+QRGI++ Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVD---KGT-TRTDNTLLERQRGITIQ 57 Query: 69 SSVMQFDYDDKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVK 128 + + F +++ +VNI+DTPGH DF + YR+L +D A++++ + G++AQT+ LF ++ Sbjct: 58 TGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117 Query: 129 HRGIPVFTFMNKLDRDGREPLDLLQELEEVLGIASYPMNWPIGMGKAFEGLYDLYNQRLE 188 GIP F+NK+D++G + + Q+++E L + + N + Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIK----------QKVELYPNMCVT 167 Query: 189 LYKGDERFASLEDGDKLFGSNPFYEQVKDDIELLNEAGNEFSEEAILAGELTPVFFGSAL 248 + E++ ++ +G+ + E+ L + L PV+ GSA Sbjct: 168 NFTESEQWDTVIEGN-----DDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAK 222 Query: 249 TNFGVQTFLETFLKFAPEPHGHKKTDGEIVDPYDKDFSGFVFKIQANMDPRHRDRIAFVR 308 N G+ +E + G VFKI+ + R R+A++R Sbjct: 223 NNIGIDNLIEVITNKFYSS----------THRGQSELCGKVFKIE--YSEK-RQRLAYIR 269 Query: 309 IVSGEFERGMSVNLPRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTG---TYQVG 365 + SG SV + K K++ + + + A +G+I+ + + +G Sbjct: 270 LYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLG 328 Query: 366 DTLTVGKNKFEFEPLPTFTPEIFMKVSAKNVMKQKSFHKGIEQLVQEG-AIQLYKNYQTG 424 DT + + + PLP + V +++ + ++ ++ Y + T Sbjct: 329 DTKLLPQRERIENPLPL----LQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATH 384 Query: 425 EYMLGAVGQLQFEVFKHRMEGEYNAEVVMSP 455 E +L +G++Q EV ++ +Y+ E+ + Sbjct: 385 EIILSFLGKVQMEVTCALLQEKYHVEIEIKE 415
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1662	ICENUCLEATIN	92	2e-20	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 91.7 bits (227), Expect = 2e-20 Identities = 133/570 (23%), Positives = 247/570 (43%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S++S + + A ++++ S++ A ++ +A ++ A ++Q+A +S+ Sbjct: 166 STLSGTHQSQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQM 225 Query: 1024 ASTSASQSAS-----TSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1078 A ++Q+ T+ S T+ S+ + S T+ S+ T+ S T+ Sbjct: 226 AGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 285 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S T+ S T+ + S+ + S T+ +S T+ S T+ S T+ S T Sbjct: 286 SDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGT 345 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S+ + S T+ S+ T+ S T+ S T+ S T+ + S+ + Sbjct: 346 AGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYG 405 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ +S T+ S T+ S T+ S T+ S+ + S T+ S+ T Sbjct: 406 STQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLT 465 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S ST+ +S+ + S T+ S T+ S T+ +E Sbjct: 466 AGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNE 525 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S + S ST+ + S+ + S T++ S T+ S T+ S T+ S T Sbjct: 526 SDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGT 585 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S+S+ + S T++ S+ T+ S T+ +S T+ S ST+ ++S+ + Sbjct: 586 AGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYG 645 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ S T+ S T+ S T+ S ST+ ++S+ + S T+ S T Sbjct: 646 STQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 705 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S T+ S TS S ST+ + S+ Sbjct: 706 AGYGSTQTAQEGSDLTSGYGSTSTAGADSS 735 Score = 91.7 bits (227), Expect = 2e-20 Identities = 133/570 (23%), Positives = 258/570 (45%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A +S+ A ++++ + +A ++ +A +S A ++Q+A +S + Sbjct: 214 STQTAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLT 273 Query: 1024 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAS-- 1081 A ++Q+A + +A ++ +A +S A ++Q+A ++++A ++Q+A Sbjct: 274 AGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKG 333 Query: 1082 ---TSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 T+ S T+ S+ + S T+ S+ T+ S T+ S T+ S T Sbjct: 334 SDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGT 393 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 + S+ + S T+ +S T+ S T+ S T+ S T+ S+ + Sbjct: 394 AGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYG 453 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S+ T+ S T+ S T+ S ST+ +S+ + S T+ S T Sbjct: 454 STQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLT 513 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ ++S + S ST+ + S+ + S T++ S T+ S T+ Sbjct: 514 AGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREG 573 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S T+ S+S+ + S T++ S+ T+ S T+ +S T+ S ST Sbjct: 574 SDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTST 633 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + ++S+ + S T+ S T+ S T+ S T+ S ST+ ++S+ + Sbjct: 634 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYG 693 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ S T+ S T+ S TS S ST+ ++S+ + S T++ S+ T Sbjct: 694 STQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLT 753 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S T+ +S T+ S ST+ + S+ Sbjct: 754 AGYGSTQTAREQSVLTTGYGSTSTAGADSS 783 Score = 91.4 bits (226), Expect = 2e-20 Identities = 132/570 (23%), Positives = 264/570 (46%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A +S +A ++++ + +A ++ +A +S A ++Q+A ++++ Sbjct: 262 STQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQT 321 Query: 1024 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTS 1083 A ++Q+A + +A ++ +A +S A ++Q+A +S +A ++Q+A Sbjct: 322 AGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 381 Query: 1084 ASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAS-----TSASKSAST 1138 + +A ++ +A +S A ++Q+A ++++A ++Q+A T+ S T Sbjct: 382 SDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGT 441 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S+ + S T+ S+ T+ S T+ S T+ S ST+ +S+ + Sbjct: 442 AGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYG 501 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S T+ ++S + S ST+ + S+ + S T++ S T Sbjct: 502 STQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLT 561 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S T+ S S+ + S T++ S+ T+ S T+ + Sbjct: 562 AGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQ 621 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S ST+ ++S+ + S T+ S T+ S T+ S T+ S ST Sbjct: 622 SVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTST 681 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + ++S+ + S T+ S T+ S T+ S TS S ST+ ++S+ + Sbjct: 682 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYG 741 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T++ S+ T+ S T+ +S T+ S ST+ ++S+ + S T+ S T Sbjct: 742 STQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILT 801 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S T+ S T+ S ST+ + S+ Sbjct: 802 AGYGSTQTAQERSDLTTGYGSTSTAGADSS 831 Score = 91.0 bits (225), Expect = 3e-20 Identities = 143/570 (25%), Positives = 254/570 (44%), Gaps = 8/570 (1%) Query: 967 SASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1026 S T+ S+ T+ ST + S T+ S ST+ +S+ + S T+ S T Sbjct: 454 STQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLT 513 Query: 1027 SASQSASTSASKSASTSASQSASTSASKS---ASTSASQSASTSASKSASTSASQSAS-- 1081 + S T+ ++S + S ST+ + S A ++Q+AS ++ +A ++Q+A Sbjct: 514 AGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREG 573 Query: 1082 ---TSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 T+ S T+ S S+ + S T++ S+ T+ S T+ QS T+ S ST Sbjct: 574 SDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTST 633 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 + S+ + S T+ S T+ S T+ S T+ S ST+ + S+ + Sbjct: 634 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYG 693 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S T+ S TS S ST+ + S+ + S T++ S+ T Sbjct: 694 STQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLT 753 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ +S T+ S ST+ + S+ + S T+ S T+ S T+ Sbjct: 754 AGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQER 813 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S ST+ ++S+ + S T+ S T+ S T+ S T+ S ST Sbjct: 814 SDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTST 873 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + +S+ + S T+ S T+ S T+ S T+ S ST+ ES+ + Sbjct: 874 AGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYG 933 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T++ +S + S+ T+ +S+ T+ S S + +S+ + S T+ +S T Sbjct: 934 STQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLT 993 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S T+ S T+ S +T+ + S+ Sbjct: 994 AGYGSTQTAEHSSTLTAGYGSTATAGADSS 1023 Score = 91.0 bits (225), Expect = 3e-20 Identities = 143/570 (25%), Positives = 254/570 (44%), Gaps = 8/570 (1%) Query: 967 SASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1026 S T+ S+ + ST + S+ T+ S T+ S T+ S ST+ +S+ Sbjct: 438 STGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLI 497 Query: 1027 SASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKS---ASTSASQSASTS 1083 + S T+ S T+ S T+ ++S + S ST+ + S A ++Q+AS + Sbjct: 498 AGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYN 557 Query: 1084 ASKSASTSASQSAS-----TSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 + +A ++Q+A T+ S T+ S S+ + S T++ S+ T+ S T Sbjct: 558 SVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQT 617 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 QS T+ S ST+ + S+ + S T+ S T+ S T+ S T+ Sbjct: 618 AREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYG 677 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S ST+ + S+ + S T+ S T+ S T+ S TS S ST+ + S+ Sbjct: 678 STSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLI 737 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T++ S+ T+ S T+ +S T+ S ST+ ++S+ + S T+ Sbjct: 738 AGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYH 797 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S T+ S T+ S ST+ ++S+ + S T+ S T+ S T Sbjct: 798 SILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQT 857 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S T+ S ST+ +S+ + S T+ S T+ S T+ S T+ Sbjct: 858 AQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYG 917 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S ST+ ES+ + S T++ +S + S+ T+ +S+ T+ S S + +S+ Sbjct: 918 STSTAGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLI 977 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S T+ +S T+ S T+ +ST Sbjct: 978 AGYGSTQTAGYQSTLTAGYGSTQTAEHSST 1007 Score = 90.6 bits (224), Expect = 4e-20 Identities = 130/570 (22%), Positives = 255/570 (44%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A ++ A ++++ +S+ A ++Q+ + +A ++ +A +S Sbjct: 198 STGTAGADSTLVAGYGSTQTAGEESSQMAGYGSTQTGMKGSDLTAGYGSTGTAGDDSSLI 257 Query: 1024 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTS 1083 A ++Q+A +S +A ++Q+A + +A ++ +A +S A ++Q+A Sbjct: 258 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 317 Query: 1084 ASKSASTSASQSAS-----TSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 ++++A ++Q+A T+ S T+ S+ + S T+ S+ T+ S T Sbjct: 318 STQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQT 377 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S T+ S T+ + S+ + S T+ +S T+ S T+ S T+ Sbjct: 378 AQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYG 437 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S+ + S T+ S+ T+ S T+ S T+ S ST+ +S+ Sbjct: 438 STGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLI 497 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S T+ ++S + S ST+ + S+ + S T++ Sbjct: 498 AGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYN 557 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S T+ S T+ S T+ S+S+ + S T++ S+ T+ S T Sbjct: 558 SVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQT 617 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + +S T+ S ST+ ++S+ + S T+ S T+ S T+ S T+ Sbjct: 618 AREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYG 677 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S ST+ ++S+ + S T+ S T+ S T+ S TS S ST+ ++S+ Sbjct: 678 STSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLI 737 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S T++ S+ T+ S T+ S Sbjct: 738 AGYGSTQTASYHSSLTAGYGSTQTAREQSV 767 Score = 90.2 bits (223), Expect = 4e-20 Identities = 132/570 (23%), Positives = 261/570 (45%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A + +A ++ + +S A ++Q+A ++++A ++Q+A + + Sbjct: 278 STQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLT 337 Query: 1024 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTS 1083 A ++ +A +S A ++Q+A +S +A ++Q+A + +A ++ +A Sbjct: 338 AGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGAD 397 Query: 1084 ASKSASTSASQSASTSASKSASTSASQSAS-----TSASKSASTSASQSASTSASKSAST 1138 +S A ++Q+A ++++A ++Q+A T+ S T+ S+ + S T Sbjct: 398 SSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQT 457 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S+ T+ S T+ S T+ S ST+ +S+ + S T+ S T+ Sbjct: 458 AGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYG 517 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ ++S + S ST+ + S+ + S T++ S T+ S T+ S T Sbjct: 518 STQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLT 577 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S S+ + S T++ S+ T+ S T+ +S T+ S ST+ ++ Sbjct: 578 AGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGAD 637 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S+ + S T+ S T+ S T+ S T+ S ST+ ++S+ + S T Sbjct: 638 SSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQT 697 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S T+ S T+ S TS S ST+ ++S+ + S T++ S+ T+ Sbjct: 698 AGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYG 757 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ +S T+ S ST+ ++S+ + S T+ S T+ S T+ S T Sbjct: 758 STQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLT 817 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S ST+ ++S+ + S T+ S Sbjct: 818 TGYGSTSTAGADSSLIAGYGSTQTAGYNSI 847 Score = 89.4 bits (221), Expect = 7e-20 Identities = 137/577 (23%), Positives = 259/577 (44%), Gaps = 5/577 (0%) Query: 956 ASTSLSKSSSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQS 1015 +S + S+ +A + +A ++ + +S A ++Q+A ++ +A ++Q+ Sbjct: 462 SSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQT 521 Query: 1016 ASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAS-----TSASK 1070 A + ++ +A ++S A ++Q+AS ++ +A ++Q+A T+ Sbjct: 522 AQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYG 581 Query: 1071 SASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAST 1130 S T+ S S+ + S T++ S+ T+ S T+ QS T+ S ST+ + S+ Sbjct: 582 STGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLI 641 Query: 1131 SASKSASTKASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASK 1190 + S T S T+ S T+ S T+ S ST+ + S+ + S T+ Sbjct: 642 AGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYN 701 Query: 1191 SASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAST 1250 S T+ S T+ S TS S ST+ + S+ + S T++ S+ T+ S T Sbjct: 702 SILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQT 761 Query: 1251 SASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASA 1310 + +S T+ S ST+ + S+ + S T+ S T+ S T+ S T+ Sbjct: 762 AREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYG 821 Query: 1311 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESAST 1370 S ST+ ++S+ + S T+ S T+ S T+ S T+ S ST+ +S+ Sbjct: 822 STSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLI 881 Query: 1371 SASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASE 1430 + S T+ S T+ S T+ S T+ S ST+ ES+ + S T++ + Sbjct: 882 AGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFK 941 Query: 1431 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESAST 1490 S + S+ T+ +S+ T+ S S + +S+ + S T+ +S T+ S T Sbjct: 942 STLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQT 1001 Query: 1491 SASESASTSASASASTSASESASTSASASASTSASAS 1527 + S T+ S +T+ ++S+ + S+ TS S Sbjct: 1002 AEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRS 1038 Score = 89.4 bits (221), Expect = 7e-20 Identities = 137/570 (24%), Positives = 253/570 (44%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A ++ +A ++++ + ++ +A ++S A ++Q+AS ++ + Sbjct: 502 STQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLT 561 Query: 1024 ASTSASQSAS-----TSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1078 A ++Q+A T+ S T+ S S+ + S T++ S+ T+ S T+ Q Sbjct: 562 AGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQ 621 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S T+ S ST+ + S+ + S T+ S T+ S T+ S T+ S ST Sbjct: 622 SVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTST 681 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 + S+ + S T+ S T+ S T+ S TS S ST+ + S+ + Sbjct: 682 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYG 741 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T++ S+ T+ S T+ +S T+ S ST+ + S+ + S T+ S T Sbjct: 742 STQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILT 801 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ +S T+ S ST+ + S+ + S T+ S T+ S T+ Sbjct: 802 AGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEN 861 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S ST+ +S+ + S T+ S T+ S T+ S T+ S ST Sbjct: 862 SDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTST 921 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + ES+ + S T++ +S + S+ T+ +S+ T+ S S + +S+ + Sbjct: 922 AGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYG 981 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ +S T+ S T+ S T+ S +T+ ++S+ + S+ TS S T Sbjct: 982 STQTAGYQSTLTAGYGSTQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLT 1041 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S S S T+ S+ S S+ Sbjct: 1042 AGYGSTLISGLRSVLTAGYGSSLISGRRSS 1071 Score = 89.4 bits (221), Expect = 8e-20 Identities = 129/569 (22%), Positives = 265/569 (46%), Gaps = 5/569 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ + + +A ++ + +S A ++Q+A +S +A ++Q+A + + Sbjct: 230 STQTGMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLT 289 Query: 1024 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTS 1083 A ++ +A +S A ++Q+A ++++A ++Q+A + +A ++ +A Sbjct: 290 AGYGSTGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDD 349 Query: 1084 ASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTKASQS 1143 +S A ++Q+A +S +A ++Q+A + +A ++ +A +S A ++Q+ Sbjct: 350 SSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQT 409 Query: 1144 ASTSASQSASTSASKSAS-----TSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 A ++Q+A ++++A T+ S T+ S+ + S T+ S+ T+ Sbjct: 410 AGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYG 469 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S ST+ +S+ + S T+ S T+ S T+ ++S Sbjct: 470 STQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLI 529 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S ST+ + S+ + S T++ S T+ S T+ S T+ S T+ S+ Sbjct: 530 TGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSD 589 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S+ + S T++ S+ T+ S T+ +S T+ S ST+ ++S+ + S T Sbjct: 590 SSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQT 649 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S T+ S T+ S T+ S ST+ ++S+ + S T+ S T+ Sbjct: 650 AGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYG 709 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ S TS S ST+ ++S+ + S T++ S+ T+ S T+ +S T Sbjct: 710 STQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLT 769 Query: 1499 SASASASTSASESASTSASASASTSASAS 1527 + S ST+ ++S+ + S T+ S Sbjct: 770 TGYGSTSTAGADSSLIAGYGSTQTAGYHS 798 Score = 89.4 bits (221), Expect = 9e-20 Identities = 141/573 (24%), Positives = 249/573 (43%), Gaps = 6/573 (1%) Query: 959 SLSKSSSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSAST 1018 S + S+ T+ S T+ S + S T+ + S+ + S T+ +S T Sbjct: 358 STQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEESTQT 417 Query: 1019 SASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1078 + S T+ S T+ S T+ S+ + S T+ S+ T+ S T+ Sbjct: 418 AGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKG 477 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S T+ S ST+ +S+ + S T+ S T+ S T+ ++S + S ST Sbjct: 478 SDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTST 537 Query: 1139 KASQS---ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTS 1195 + S A ++Q+AS ++ +A ++Q+A S T+ S T+ S S+ + Sbjct: 538 AGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREG---SDLTAGYGSTGTAGSDSSIIA 594 Query: 1196 ASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1255 S T++ S+ T+ S T+ +S T+ S ST+ + S+ + S T+ S Sbjct: 595 GYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNS 654 Query: 1256 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTS 1315 T+ S T+ S T+ S ST+ + S+ + S T+ S T+ S T+ Sbjct: 655 ILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTA 714 Query: 1316 ASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASAS 1375 S TS S ST+ ++S+ + S T++ S+ T+ S T+ +S T+ S Sbjct: 715 QEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGS 774 Query: 1376 ASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTS 1435 ST+ ++S+ + S T+ S T+ S T+ S T+ S ST+ ++S+ + Sbjct: 775 TSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIA 834 Query: 1436 ASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASES 1495 S T+ S T+ S T+ S T+ S ST+ +S+ + S T+ S Sbjct: 835 GYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNS 894 Query: 1496 ASTSASASASTSASESASTSASASASTSASAST 1528 T+ S T+ S T+ S ST+ S+ Sbjct: 895 ILTAGYGSTQTAQENSDLTTGYGSTSTAGYESS 927 Score = 89.4 bits (221), Expect = 9e-20 Identities = 137/570 (24%), Positives = 255/570 (44%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A +S A ++++ ++ +A ++Q+A + ++ +A ++S Sbjct: 486 STSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGANSSLI 545 Query: 1024 ASTSASQSASTSASKSASTSASQSAS-----TSASKSASTSASQSASTSASKSASTSASQ 1078 A ++Q+AS ++ +A ++Q+A T+ S T+ S S+ + S T++ Sbjct: 546 AGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYH 605 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S+ T+ S T+ QS T+ S ST+ + S+ + S T+ S T+ S T Sbjct: 606 SSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQT 665 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S T+ S ST+ + S+ + S T+ S T+ S T+ S TS Sbjct: 666 AQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYG 725 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S ST+ + S+ + S T++ S+ T+ S T+ +S T+ S ST+ + S+ Sbjct: 726 STSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLI 785 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S T+ +S T+ S ST+ ++S+ + S T+ Sbjct: 786 AGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYN 845 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S T+ S T+ S ST+ +S+ + S T+ S T+ S T Sbjct: 846 SILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQT 905 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S T+ S ST+ ES+ + S T++ +S + S+ T+ +S+ T+ Sbjct: 906 AQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYG 965 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S S + +S+ + S T+ +S T+ S T+ S T+ S +T+ ++S+ Sbjct: 966 STSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQTAEHSSTLTAGYGSTATAGADSSLI 1025 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S+ TS S T+ S S S Sbjct: 1026 AGYGSSLTSGIRSFLTAGYGSTLISGLRSV 1055 Score = 89.0 bits (220), Expect = 1e-19 Identities = 138/562 (24%), Positives = 247/562 (43%), Gaps = 6/562 (1%) Query: 970 TSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSAS 1029 T+ S T+ S+ ++ S T+ S+ T+ S T+ S T+ S T+ + Sbjct: 337 TAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGA 396 Query: 1030 QSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAS 1089 S+ + S T+ +S T+ S T+ S T+ S T+ S+ + S Sbjct: 397 DSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQ 456 Query: 1090 TSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTKASQSASTSAS 1149 T+ S+ T+ S T+ S T+ S ST+ +S+ + S T S T+ Sbjct: 457 TAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGY 516 Query: 1150 QSASTSASKSASTSASQSASTSASKS---ASTSASQSASTSASKSASTSASQSASTSASK 1206 S T+ ++S + S ST+ + S A ++Q+AS ++ +A ++Q+A Sbjct: 517 GSTQTAQNESDLITGYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREG--- 573 Query: 1207 SASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAST 1266 S T+ S T+ S S+ + S T++ S+ T+ S T+ +S T+ S ST Sbjct: 574 SDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTST 633 Query: 1267 SASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASESASTSASA 1326 + + S+ + S T+ S T+ S T+ S T+ S ST+ ++S+ + Sbjct: 634 AGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYG 693 Query: 1327 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESAST 1386 S T+ S T+ S T+ S TS S ST+ ++S+ + S T++ S+ T Sbjct: 694 STQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLT 753 Query: 1387 SASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASE 1446 + S T+ +S T+ S ST+ ++S+ + S T+ S T+ S T+ Sbjct: 754 AGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQER 813 Query: 1447 SASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESASTSASASAST 1506 S T+ S ST+ ++S+ + S T+ S T+ S T+ S T+ S ST Sbjct: 814 SDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTST 873 Query: 1507 SASESASTSASASASTSASAST 1528 + +S+ + S T+ S Sbjct: 874 AGYDSSLIAGYGSTQTAGYNSI 895 Score = 88.7 bits (219), Expect = 1e-19 Identities = 142/570 (24%), Positives = 251/570 (44%), Gaps = 8/570 (1%) Query: 967 SASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1026 S T+ S T+ ST + S+ + S T+ S+ T+ S T+ S T Sbjct: 422 STQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLT 481 Query: 1027 SASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASK 1086 + S ST+ +S+ + S T+ S T+ S T+ ++S + S ST+ + Sbjct: 482 AGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYGSTSTAGAN 541 Query: 1087 SASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKS---ASTKASQS 1143 S+ + S T++ S T+ S T+ S T+ S T+ S S A ++Q+ Sbjct: 542 SSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQT 601 Query: 1144 ASTSASQSASTSASKSAS-----TSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 AS +S +A ++++A T+ S ST+ + S+ + S T+ S T+ Sbjct: 602 ASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYG 661 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S ST+ + S+ + S T+ S T+ S T+ S T Sbjct: 662 STQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLT 721 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 S S ST+ + S+ + S T++ S+ T+ S T+ +S T+ S ST+ ++ Sbjct: 722 SGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGAD 781 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S+ + S T+ S T+ S T+ S T+ S ST+ ++S+ + S T Sbjct: 782 SSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQT 841 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S T+ S T+ S T+ S ST+ +S+ + S T+ S T+ Sbjct: 842 AGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYG 901 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ S T+ S ST+ ES+ + S T++ +S + S+ T+ +S+ T Sbjct: 902 STQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLT 961 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S S + +S+ + S T+ ST Sbjct: 962 AGYGSTSMAGYDSSLIAGYGSTQTAGYQST 991 Score = 88.7 bits (219), Expect = 1e-19 Identities = 132/577 (22%), Positives = 252/577 (43%), Gaps = 7/577 (1%) Query: 956 ASTSLSKSSSISASTSASKSASTSASKSTSVSAS-KSASTSASQSASTSASKSASTSASQ 1014 A T + TS K + S + + A+ +S ST +Q+ + S + Q Sbjct: 114 ACTEMQAGPGSPDVTSEVKVGNRSLPVTDDIDATIESGSTQPTQTIEIATYGSTLSGTHQ 173 Query: 1015 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1074 S + S T+ S + S T+ + S + S T+ +S+ + S T Sbjct: 174 SQLIAGYGSTETAGDSSTLIAGYGSTGTAGADSTLVAGYGSTQTAGEESSQMAGYGSTQT 233 Query: 1075 SASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASK 1134 S T+ S T+ S+ + S T+ S+ T+ S T+ S T+ Sbjct: 234 GMKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYG 293 Query: 1135 SASTKASQS---ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1191 S T + S A ++Q+A ++++A ++Q+A + +A ++ +A +S Sbjct: 294 STGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLI 353 Query: 1192 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTS 1251 A ++Q+A +S +A ++Q+A + +A ++ +A +S A ++Q+A Sbjct: 354 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 413 Query: 1252 ASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASAS 1311 ++++A ++Q+A S T+ S T+ S+ + S T+ +S+ T+ S Sbjct: 414 STQTAGYGSTQTAQKG---SDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGS 470 Query: 1312 ASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTS 1371 T+ S T+ S ST+ ES+ + S T+ S T+ S T+ +ES + Sbjct: 471 TQTAQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLIT 530 Query: 1372 ASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASES 1431 S ST+ + S+ + S T++ S T+ S T+ S T+ S T+ S+S Sbjct: 531 GYGSTSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDS 590 Query: 1432 ASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTS 1491 + + S T++ S+ T+ S T+ +S T+ S ST+ ++S+ + S T+ Sbjct: 591 SIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTA 650 Query: 1492 ASESASTSASASASTSASESASTSASASASTSASAST 1528 S T+ S T+ S T+ S ST+ + S+ Sbjct: 651 GYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSS 687 Score = 87.1 bits (215), Expect = 4e-19 Identities = 139/572 (24%), Positives = 250/572 (43%), Gaps = 6/572 (1%) Query: 959 SLSKSSSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSAST 1018 S + + S+ + S T+ +ST + S T+ S T+ S T+ S+ Sbjct: 294 STGTAGADSSLIAGYGSTQTAGEESTQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLI 353 Query: 1019 SASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1078 + S T+ S+ T+ S T+ S T+ S T+ + S+ + S T+ + Sbjct: 354 AGYGSTQTAGEDSSLTAGYGSTQTAQKGSDLTAGYGSTGTAGADSSLIAGYGSTQTAGEE 413 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S T+ S T+ S T+ S T+ S+ + S T+ S+ T+ S T Sbjct: 414 STQTAGYGSTQTAQKGSDLTAGYGSTGTAGDDSSLIAGYGSTQTAGEDSSLTAGYGSTQT 473 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S T+ S ST+ +S+ + S T+ S T+ S T+ ++S + Sbjct: 474 AQKGSDLTAGYGSTSTAGYESSLIAGYGSTQTAGYGSTLTAGYGSTQTAQNESDLITGYG 533 Query: 1199 SASTSASKS---ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1255 S ST+ + S A ++Q+AS ++ +A ++Q+A S T+ S T+ S S Sbjct: 534 STSTAGANSSLIAGYGSTQTASYNSVLTAGYGSTQTAREG---SDLTAGYGSTGTAGSDS 590 Query: 1256 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTS 1315 + + S T++ S+ T+ S T+ +S T+ S ST+ ++S+ + S T+ Sbjct: 591 SIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTA 650 Query: 1316 ASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASAS 1375 S T+ S T+ S T+ S ST+ ++S+ + S T+ S T+ S Sbjct: 651 GYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGS 710 Query: 1376 ASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTS 1435 T+ S TS S ST+ ++S+ + S T++ S+ T+ S T+ +S T+ Sbjct: 711 TQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTT 770 Query: 1436 ASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASES 1495 S ST+ ++S+ + S T+ S T+ S T+ S T+ S ST+ ++S Sbjct: 771 GYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADS 830 Query: 1496 ASTSASASASTSASESASTSASASASTSASAS 1527 + + S T+ S T+ S T+ S Sbjct: 831 SLIAGYGSTQTAGYNSILTAGYGSTQTAQENS 862 Score = 84.8 bits (209), Expect = 2e-18 Identities = 136/570 (23%), Positives = 252/570 (44%), Gaps = 5/570 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +AS ++ +A ++++ + +A ++ +A + +S A ++Q+AS +S + Sbjct: 550 STQTASYNSVLTAGYGSTQTAREGSDLTAGYGSTGTAGSDSSIIAGYGSTQTASYHSSLT 609 Query: 1024 ASTSASQSAS-----TSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1078 A ++Q+A T+ S ST+ + S+ + S T+ S T+ S T+ Sbjct: 610 AGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEG 669 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S T+ S ST+ + S+ + S T+ S T+ S T+ S TS S ST Sbjct: 670 SDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTSGYGSTST 729 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 + S+ + S T++ S+ T+ S T+ +S T+ S ST+ + S+ + Sbjct: 730 AGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYG 789 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S T+ +S T+ S ST+ + S+ + S T+ S T Sbjct: 790 STQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 849 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S ST+ S+ + S T+ S T+ S T+ Sbjct: 850 AGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEN 909 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S T+ S ST+ ES+ + S T++ +S + S+ T+ +S+ T+ S S Sbjct: 910 SDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLTAGYGSTSM 969 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + +S+ + S T+ +S T+ S T+ S T+ S +T+ ++S+ + Sbjct: 970 AGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQTAEHSSTLTAGYGSTATAGADSSLIAGYG 1029 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S+ TS S T+ S S S T+ S+ S S+ T+ S ++ S+ Sbjct: 1030 SSLTSGIRSFLTAGYGSTLISGLRSVLTAGYGSSLISGRRSSLTAGYGSNQIASHRSSLI 1089 Query: 1499 SASASASTSASESASTSASASASTSASAST 1528 + S + + S + S+ T+ ST Sbjct: 1090 AGPESTQITGNRSMLIAGKGSSQTAGYRST 1119 Score = 80.2 bits (197), Expect = 5e-17 Identities = 137/569 (24%), Positives = 244/569 (42%), Gaps = 8/569 (1%) Query: 967 SASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1026 S T+ S S+ + ST ++ S+ T+ S T+ +S T+ S ST+ + S+ Sbjct: 582 STGTAGSDSSIIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLI 641 Query: 1027 SASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASK 1086 + S T+ S T+ S T+ S T+ S ST+ + S+ + S T+ Sbjct: 642 AGYGSTQTAGYNSILTAGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYN 701 Query: 1087 SASTSASQSASTSASKSASTSASQSASTSASKS---ASTSASQSASTSASKSASTKASQS 1143 S T+ S T+ S TS S ST+ + S A ++Q+AS +S +A ++Q+ Sbjct: 702 SILTAGYGSTQTAQEGSDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQT 761 Query: 1144 AS-----TSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 A T+ S ST+ + S+ + S T+ S T+ S T+ +S T+ Sbjct: 762 AREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYG 821 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S ST+ + S+ + S T+ S T+ S T+ S T+ S ST+ S+ Sbjct: 822 STSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLI 881 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S T+ S T+ S ST+ ES+ + S T++ + Sbjct: 882 AGYGSTQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFK 941 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S + S+ T+ +S+ T+ S S + +S+ + S T+ +S T+ S T Sbjct: 942 STLMAGYGSSQTAREQSSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQT 1001 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + S T+ S +T+ ++S+ + S+ TS S T+ S S S T+ Sbjct: 1002 AEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGYG 1061 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S+ S S+ T+ S ++ S+ + S + + S + S+ T+ S Sbjct: 1062 SSLISGRRSSLTAGYGSNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTLI 1121 Query: 1499 SASASASTSASESASTSASASASTSASAS 1527 S + S + + + S T+ S Sbjct: 1122 SGADSVQMAGERGKLIAGADSTQTAGDRS 1150 Score = 78.6 bits (193), Expect = 2e-16 Identities = 135/569 (23%), Positives = 243/569 (42%), Gaps = 8/569 (1%) Query: 967 SASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1026 S T++ S+ T+ ST + +S T+ S ST+ + S+ + S T+ S T Sbjct: 598 STQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGADSSLIAGYGSTQTAGYNSILT 657 Query: 1027 SASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASK 1086 + S T+ S T+ S ST+ + S+ + S T+ S T+ S T+ Sbjct: 658 AGYGSTQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEG 717 Query: 1087 SASTSASQSASTSASKS---ASTSASQSASTSASKSASTSASQSAS-----TSASKSAST 1138 S TS S ST+ + S A ++Q+AS +S +A ++Q+A T+ S ST Sbjct: 718 SDLTSGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTST 777 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 + S+ + S T+ S T+ S T+ +S T+ S ST+ + S+ + Sbjct: 778 AGADSSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYG 837 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S T+ S T+ S ST+ S+ + S T+ S T Sbjct: 838 STQTAGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILT 897 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S T+ S T+ S ST+ +S+ + S T++ +S + S+ T+ + Sbjct: 898 AGYGSTQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQ 957 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S+ T+ S S + +S+ + S T+ +S T+ S T+ S T+ S +T Sbjct: 958 SSLTAGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQTAEHSSTLTAGYGSTAT 1017 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 + ++S+ + S+ TS S T+ S S S T+ S+ S S+ T+ Sbjct: 1018 AGADSSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGYGSSLISGRRSSLTAGYG 1077 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S ++ S+ + S + + S + S+ T+ S S ++S + Sbjct: 1078 SNQIASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGADSVQMAGERGKLI 1137 Query: 1499 SASASASTSASESASTSASASASTSASAS 1527 + + S T+ S + + S T+ S Sbjct: 1138 AGADSTQTAGDRSKLLAGNNSYLTAGDRS 1166 Score = 70.2 bits (171), Expect = 6e-14 Identities = 120/553 (21%), Positives = 233/553 (42%), Gaps = 5/553 (0%) Query: 964 SSISASTSASKSASTSASKSTSVSASKSASTSASQSASTSASKSASTSASQSASTSASKS 1023 S+ +A + +A ++ + +S A ++Q+A ++ +A ++Q+A + + Sbjct: 662 STQTAQEGSDLTAGYGSTSTAGADSSLIAGYGSTQTAGYNSILTAGYGSTQTAQEGSDLT 721 Query: 1024 ASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSAS-----TSASKSASTSASQ 1078 + ++ +A +S A ++Q+AS +S +A ++Q+A T+ S ST+ + Sbjct: 722 SGYGSTSTAGADSSLIAGYGSTQTASYHSSLTAGYGSTQTAREQSVLTTGYGSTSTAGAD 781 Query: 1079 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1138 S+ + S T+ S T+ S T+ +S T+ S ST+ + S+ + S T Sbjct: 782 SSLIAGYGSTQTAGYHSILTAGYGSTQTAQERSDLTTGYGSTSTAGADSSLIAGYGSTQT 841 Query: 1139 KASQSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQ 1198 S T+ S T+ S T+ S ST+ S+ + S T+ S T+ Sbjct: 842 AGYNSILTAGYGSTQTAQENSDLTTGYGSTSTAGYDSSLIAGYGSTQTAGYNSILTAGYG 901 Query: 1199 SASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSASTSASQSASTSASKSAST 1258 S T+ S T+ S ST+ +S+ + S T++ KS + S+ T+ +S+ T Sbjct: 902 STQTAQENSDLTTGYGSTSTAGYESSLIAGYGSTQTASFKSTLMAGYGSSQTAREQSSLT 961 Query: 1259 SASQSASTSASKSASTSASQSASTSASKSASTSASASASTSASESASTSASASASTSASE 1318 + S S + S+ + S T+ +S T+ S T+ S T+ S +T+ ++ Sbjct: 962 AGYGSTSMAGYDSSLIAGYGSTQTAGYQSTLTAGYGSTQTAEHSSTLTAGYGSTATAGAD 1021 Query: 1319 SASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASASAST 1378 S+ + S+ TS S T+ S S S T+ S+ S S+ T+ S Sbjct: 1022 SSLIAGYGSSLTSGIRSFLTAGYGSTLISGLRSVLTAGYGSSLISGRRSSLTAGYGSNQI 1081 Query: 1379 SASESASTSASASASTSASESASTSASASASTSASESASTSASASASTSASESASTSASA 1438 ++ S+ + S + + S + S+ T+ S S + S + + + Sbjct: 1082 ASHRSSLIAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGADSVQMAGERGKLIAGAD 1141 Query: 1439 SASTSASESASTSASASASTSASESASTSASASASTSASESASTSASESASTSASESAST 1498 S T+ S + + S T+ S T+ + + S T+ S T+ S Sbjct: 1142 STQTAGDRSKLLAGNNSYLTAGDRSKLTAGNDCILMAGDRSKLTAGINSILTAGCRSKLI 1201 Query: 1499 SASASASTSASES 1511 ++ S T+ S Sbjct: 1202 GSNGSTLTAGENS 1214
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1653	SECYTRNLCASE	139	3e-39	Preprotein translocase SecY subunit signature.
>SECYTRNLCASE#Preprotein translocase SecY subunit signature. Length = 437 Score = 139 bits (352), Expect = 3e-39 Identities = 85/387 (21%), Positives = 175/387 (45%), Gaps = 40/387 (10%) Query: 11 KKGLYTLFLLFIYVLGSRITLPFVDLNSKDFL----GGSTAYLAFSAALTGGNLRSLSIF 66 KK L+TL ++ +Y +G+ I +P VD + G+ +GG L ++IF Sbjct: 16 KKLLFTLAIIVVYRVGTHIPIPGVDYKNVQQCVREASGNQGLFGLVNMFSGGALLQITIF 75 Query: 67 SVGLSPWMSAMILWQMFSYS----KKLGLSSTAIEIQDRRKM-----YLTLLIAVIQSLA 117 ++G+ P+++A I+ Q+ + + L A K+ YLT+ +A++Q Sbjct: 76 ALGIMPYITASIILQLLTVVIPRLEALKKEGQA----GTAKITQYTRYLTVALAILQGTG 131 Query: 118 VSLSLPVQSSY------------SAILVVLMNTLLLIAGTFFLVWLSDLNASMGIG-GSI 164 + + + +I + + + AGT ++WL +L GIG G Sbjct: 132 LVATARSAPLFGRCSVGGQIVPDQSIFTTITMVICMTAGTCVVMWLGELITDRGIGNGMS 191 Query: 165 VILLSSIVLNIPQDVIETFQTVHIPTGIIVLLALLTLVFSYLLAIMY--RARYLVPVN-- 220 +++ SI P + + + G I ++ + + +++ +A+ +PV Sbjct: 192 ILMFISIAATFPSALWAIKKQGTLAGGWIEFGTVIAVGLIMVALVVFVEQAQRRIPVQYA 251 Query: 221 --KIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHAGLAALSK 278 IG + +Y+ + +N AG +P ++ S L +PA + F N + + + Sbjct: 252 KRMIGRRSYGGTSTYIPLKVNQAGVIPVIFASSLLYIPALV---AQFAGGNSGWKSWVEQ 308 Query: 279 EFMVG-KPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGEDTSRFIN 337 G P+++ Y ++ F+ + ++ N EE+AD MKK G +I GI G T+ +++ Sbjct: 309 NLTKGDHPIYIVTYFLLIVFFAFFYVAISFNPEEVADNMKKYGGFIPGIRAGRPTAEYLS 368 Query: 338 GLVLRFSVIGGLFNVIMAGGPMLFVLF 364 ++ R + G L+ ++A P + ++ Sbjct: 369 YVLNRITWPGSLYLGLIALVPTMALVG 395

smi_1637

smi_1598

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1637	0	25	3.162604	hypothetical protein
smi_1636	2	22	-2.763350	CorA-like Mg2+ transporter protein
smi_1635	0	16	-1.685108	hypothetical protein
smi_1634	0	12	-1.786964	hypothetical protein
smi_1633	-1	12	-1.529383	hypothetical protein
smi_1632	0	12	-1.999832	nicotinic acid mononucleotide
smi_1631	0	11	-0.811149	hypothetical protein
smi_1630	0	11	3.074233	hypothetical protein
smi_1629	-1	11	2.904468	hypothetical protein
smi_1628	-2	13	3.677080	hypothetical protein
smi_1627	-3	14	3.713905	hypothetical protein
smi_1626	-2	17	3.758009	hypothetical protein
smi_1625	-3	16	3.064860	hypothetical protein
smi_1624	-2	18	2.578073	hypothetical protein
smi_1623	-2	19	3.041796	guanylate kinase
smi_1622	-2	19	3.309399	DNA-dependent RNA Polymerase, omega subunit
smi_1621	-1	17	2.744314	primosomal protein N'
smi_1620	0	19	2.942857	methionyl-tRNA formyltransferase
smi_1619	0	22	3.173042	16S rRNA (cytosine(967)-C(5))-methyltransferase
smi_1618	0	23	3.494819	serine/threonine phosphatase
smi_1617	1	27	3.130412	serine/threonine protein kinase
smi_1616	1	22	0.334981	hypothetical protein
smi_1615	1	20	-2.183703	hypothetical protein
smi_1614	-1	13	-4.032451	3-hydroxy-3-methylglutaryl CoA synthase
smi_1613	0	14	-4.356129	3-hydroxy-3-methylglutaryl-coenzyme a reductase
smi_1612	0	15	-5.404927	transcriptional regulator
smi_1611	0	18	-6.031821	glycosyl hydrolases family 32
smi_1610	0	15	-5.011349	PTS system
smi_1609	-1	14	-3.620554	transcriptional regulator/sugar kinase
smi_1608	0	15	-3.566224	hypothetical protein
smi_1607	-2	17	-3.660064	hypothetical protein
smi_1606	-2	15	-2.737186	hypothetical protein
smi_1605	-1	12	-1.029643	hypothetical protein
smi_1604	-1	14	-0.123035	type I restriction-modification system DNA
smi_1603	0	24	2.720857	hypothetical protein
smi_1602	0	23	3.067510	restriction endonuclease S subunit
smi_1601	0	20	2.404796	restriction endonuclease S subunit
smi_1600	1	21	3.337072	type I restriction-modification system
smi_1599	0	18	3.708720	type I restriction-modification system, helicase
smi_1598	-1	19	3.576812	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1637	LPSBIOSNTHSS	28	0.019	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 27.9 bits (62), Expect = 0.019 Identities = 16/72 (22%), Positives = 32/72 (44%), Gaps = 5/72 (6%) Query: 27 GILGGNFNPVHNAHLVVADQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAI 86 I G+F+P+ HL + ++ + DQV + P +K+ RL+ + AI Sbjct: 3 AIYPGSFDPITFGHLDIIERGCRL--FDQVYVAVLRNP---NKQPMFSVQERLEQIAKAI 57 Query: 87 EGIEGLSIETIE 98 + +++ E Sbjct: 58 AHLPNAQVDSFE 69
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1630	RTXTOXIND	41	7e-06	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 41.4 bits (97), Expect = 7e-06 Identities = 21/192 (10%), Positives = 56/192 (29%), Gaps = 18/192 (9%) Query: 38 NAEQEATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQE 97 + R Q + L + + + +E R + +F + + + Sbjct: 143 LLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLR-LTSLIKEQFSTWQNQ 201 Query: 98 LKQIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAK----------NLDAREEQL 147 Q E L ++ + E ++ + D + + +E + Sbjct: 202 KYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKY 261 Query: 148 EEVERQKEAELERIG----ALSQAEARDIILAQTEENLTKEIASRIREAEQEVKERSDKM 203 E + ++ + A+ + EI ++R+ + + ++ Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEE---YQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318 Query: 204 AKDILVQAMQRI 215 AK+ Q I Sbjct: 319 AKNEERQQASVI 330
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1609	BONTOXILYSIN	31	0.017	Bontoxilysin signature.
>BONTOXILYSIN#Bontoxilysin signature. Length = 1196 Score = 30.6 bits (69), Expect = 0.017 Identities = 15/77 (19%), Positives = 27/77 (35%), Gaps = 1/77 (1%) Query: 404 TTILVLAKNKLENKTLFIDAS-KEFKKETNNNVLTDSNIEHIVELFSNYQNVDYKSALVG 462 T I K+ L N F K ++ N + + E+ S YQ + + S Sbjct: 787 TNINDNEKSILINSYTFKTIDFKFLDIQSIKNFFNSQVEQVMKEILSPYQLLLFASKGPN 846 Query: 463 NDVIGSEQDYNLSVSTY 479 +++I N + Sbjct: 847 SNIIEDISGKNTLIQYT 863
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1608	YERSSTKINASE	30	0.019	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 29.7 bits (66), Expect = 0.019 Identities = 42/172 (24%), Positives = 74/172 (43%), Gaps = 32/172 (18%) Query: 146 IRSSEKVFYRQVLDLFATSSDYNANSPEAKKFFATVQNKMHYAIHHNTASELIYNRIDSE 205 I E + R + D+ S+D +S EA ++H + T E +S Sbjct: 374 IAGVETAYTRFITDILGVSADSRPDSNEA---------RLHEFLSDGTIDE------ESA 418 Query: 206 KEFLGLTTFKGDLPTLSEAKVAKNYLTEKELRGLNQLVSGYLDFAERQAEREEVMTMADW 265 K+ L T G++ LS +T K+LR L+ L+ +L A + + M Sbjct: 419 KQILK-DTLTGEMSPLS---TDVRRITPKKLRELSDLLRTHLSSAATKQ-----LDMGGV 469 Query: 266 VTHVDRILLATGEDLLDNSGSISREQMEHKVDKEYKSYQAKTLSQVEKDYLK 317 ++ +D +L+A D + G + ++Q+ K + S KT +E DY+K Sbjct: 470 LSDLDTMLVAL--DKAEREGGVDKDQL-----KSFNSLILKTYRVIE-DYVK 513

smi_1560

smi_1525

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1560	1	21	3.004932	hypothetical protein
smi_1559	0	21	3.400308	triose phosphate isomerase
smi_1558	-1	20	3.835755	choline-binding protein LytC,
smi_1557	-2	19	3.400308	DPS family peroxide resistance protein
smi_1556	-1	18	3.044201	dihydrofolate reductase
smi_1555	-1	16	3.140598	hypothetical protein
smi_1554	-2	14	2.417391	ATP-dependent Clp protease ATP-binding subunit
smi_1553	-1	13	1.526376	hypothetical protein
smi_1552	-1	15	1.546870	hypothetical protein
smi_1551	-2	13	1.746548	P-loop-containing kinase
smi_1550	-1	15	2.686701	hypothetical protein
smi_1549	0	20	2.398753	hypothetical protein
smi_1548	-2	17	3.046467	thioredoxin reductase
smi_1547	-1	18	3.553946	hypothetical protein
smi_1546	-2	19	3.238602	hypothetical protein
smi_1545	-2	20	3.249563	phosphoglucosamine mutase
smi_1544	-2	19	2.795771	hypothetical protein
smi_1543	1	17	1.565527	hypothetical protein
smi_1542	2	20	0.934557	4-hydroxy-tetrahydrodipicolinate reductase
smi_1541	2	19	0.786079	tRNA nucleotidyltransferase
smi_1540	2	18	0.361601	ATPase component of ABC transporter with
smi_1539	2	16	0.261605	cation efflux family protein
smi_1538	2	16	0.277201	yybP-ykoY element
smi_1537	1	14	-0.326347	cation-transporting ATPase, E1-E2 family
smi_1536	1	17	0.535620	polypeptide deformylase
smi_1535	1	19	0.694375	hypothetical protein
smi_1534	1	19	0.847732	hypothetical protein
smi_1533	2	23	1.316970	hypothetical protein
smi_1532	2	22	1.684477	cell wall surface anchor family protein
smi_1531	2	23	2.587496	N-acetyl-beta-hexosaminidase
smi_1530	0	14	2.525274	transposase
smi_1529	-1	17	2.955842	transposase, IS1167
smi_1528	-1	18	3.666721	beta-galactosidase
smi_1527	-1	19	3.887869	hypothetical protein
smi_1526	3	23	2.965771	transcriptional regulator
smi_1525	2	19	2.446255	cell wall surface anchor family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1555	FERRIBNDNGPP	29	0.019	Ferrichrome-binding periplasmic protein signature.
>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature. Length = 296 Score = 29.1 bits (65), Expect = 0.019 Identities = 18/65 (27%), Positives = 25/65 (38%), Gaps = 3/65 (4%) Query: 141 GTEVAGESHIADHPGMIDHVYVTNTLNDDTPLASRRVVQTILESDMIVLGPGSLFTSILP 200 + A E+H+A + I + PL + I M+V GP SLF IL Sbjct: 146 NLQSAAETHLAQYEDFIRSMKPRFVKRGARPLL---LTTLIDPRHMLVFGPNSLFQEILD 202 Query: 201 NIVIK 205 I Sbjct: 203 EYGIP 207
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1540	FLGMRINGFLIF	30	3e-04	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 29.9 bits (67), Expect = 3e-04 Identities = 9/28 (32%), Positives = 14/28 (50%) Query: 32 KKDKFLSILTSLAGIALVLVAVWLGWPK 59 ++ F+ L + LVLV W+ W K Sbjct: 450 QQQSFIDQLLAAGRWLLVLVVAWILWRK 477
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1538	V8PROTEASE	74	1e-15	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 74.3 bits (182), Expect = 1e-15 Identities = 68/344 (19%), Positives = 127/344 (36%), Gaps = 40/344 (11%) Query: 86 PKTEEELLAKEKETATSSAVSDTLPEELRGKLNKAEENGRTASKEELEKEDK--SLVPED 143 T L++ A SS D P++ + + + + + + LE+ + ++P + Sbjct: 15 TLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNN 74 Query: 144 ----VAKTKNGVLNYGATVEIKSSAG----LGSGIVIGENLVLSVSHNFIKDVPDGNNRK 195 + T NG +Y I+ A + SG+V+G++ +L+ H D + Sbjct: 75 DRHQITDTTNG--HYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPH-AL 131 Query: 196 VADNVESDGDVYTVSYKGAPDVKFSKNDVKHWDREGFLKGYKNDLAIVKL------RTPL 249 A + D Y P+ F+ + + EG DLAIVK + Sbjct: 132 KAFPSAINQDNY-------PNGGFTAEQITKYSGEG-------DLAIVKFSPNEQNKHIG 177 Query: 250 ANAPVEVIDKPSTIKVGDKVHVFGYPKGELDPILNTTVEDINNHGEGVRGISYQGS-EPG 308 + + +V + V GYP + + + I + Y S G Sbjct: 178 EVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKIT--YLKGEAMQYDLSTTGG 235 Query: 309 ASGGGIFDENGKLIGIHQNGVSGKRSGGILFSPAQLEWIQNYIKGIETTKPAGLDALDKQ 368 SG +F+E ++IGIH GV + +G + + ++N++K D Sbjct: 236 NSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINEN----VRNFLKQNIEDIHFANDDQPNN 291 Query: 369 VEDKEEKPKEDKPQEEKPADNKPAENKPADNKPAENKPADNKPA 412 ++ + D P +N N P + +N +DN A Sbjct: 292 PDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDA 335
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1537	GPOSANCHOR	39	4e-04	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 38.5 bits (89), Expect = 4e-04 Identities = 17/74 (22%), Positives = 29/74 (39%), Gaps = 4/74 (5%) Query: 11 HYSIRKFTIGAASVMIGASIFGAGMVQA----AETEGTAETEGTVTQAQPLDKLPADIAA 66 HYS+RK G ASV + ++ GAG+V + ++T+ + DK + Sbjct: 9 HYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADKFEIENNT 68 Query: 67 AIEKAEASAGTVDG 80 K + Sbjct: 69 LKLKNSDLSFNNKA 82
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1534	GPOSANCHOR	50	1e-07	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 49.7 bits (118), Expect = 1e-07 Identities = 39/203 (19%), Positives = 72/203 (35%), Gaps = 5/203 (2%) Query: 11 DKRCHYSIRKFAIGVASVMIGASIFGIS-AVQAEEAASSNTQTEETTVHQAQP-LDKLPD 68 + HYS+RK G ASV + ++ G V E ++ T+++ T+ + Q DK Sbjct: 5 NTNRHYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADKFEI 64 Query: 69 DVAAAIAKADENGGR-EFVKPKSELAEDKVTKDTETTRPANDGSHELASPKVETPNKVEE 127 + K + + +K ++ ++++ E R + E AS E + + Sbjct: 65 ENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKAD 124 Query: 128 GNKAEDKQKSEEANPKPVESAVTAGTEVRDDAKKTSEKDQVKQTTDIKSSSEKTQALSKE 187 KA + + + A K EK + S K + L E Sbjct: 125 LEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAE 184 Query: 188 SSKADVEKEKQLLSDRKQDFNKD 210 KA +E + L + Sbjct: 185 --KAALEARQAELEKALEGAMNF 205
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1532	PF05043	292	1e-95	Transcriptional activator
>PF05043#Transcriptional activator Length = 493 Score = 292 bits (748), Expect = 1e-95 Identities = 201/488 (41%), Positives = 299/488 (61%), Gaps = 2/488 (0%) Query: 1 MRNLLSTKDQRQLRLMETLIQNRNWLRLHELADKLGCTERILKSDLNELRTAFPTIDIQS 60 MR+LLS K RQL L+E L +++ W ELA+ L CTER +K DL+ +++AFP + S Sbjct: 1 MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHS 60 Query: 61 SINGIMIDLNMQTSVEDVYQHFLAHSQSFQLLEYLFFNEGLPIYRTLENLHSSRANLYRL 120 S NGI I + +E VY HF HS F +LE++FFNEG + + S ++LYR+ Sbjct: 61 STNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRI 120 Query: 121 GRNITKTLSTQFQIELSFTPSEIRGNEIDIRYFYAQYFSERYYFLDWPFPYIPEEDLTEF 180 I K + QFQ E+S TP +I GNE DIRYF+AQYFSE+YYFL+WPF E L++ Sbjct: 121 ISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQL 180 Query: 181 ADFFYKITNYPMHFSIYRMYKLMLAISIYRIKNGHFIDLPNH-FYDEYYPLLMGIPNFEE 239 + YK T++PM+ S +RM KL+L ++YRIK GHF+++ F D+ LM E Sbjct: 181 LELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEG 240 Query: 240 TLVYFSEKLGLEITPDIIAQIFISFIQNNLFLDPQEFLNSLEENSEARYSYQLLSQILER 299 F + + + +++ Q+F+S+ Q F+D F+ ++++S SY LLS +++ Sbjct: 241 VAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQ 300 Query: 300 LSKQYQITFTNHDELIWHLHNTAFFESQEIFSTPILFEQKTLTIKKFEVYFPDFMASARQ 359 +S +YQI N D LIWHLHNTA QE+F+ ILF+QK TI+ F+ FP F++ ++ Sbjct: 301 ISVKYQIEIENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDVKK 360 Query: 360 ELAQYRQAIGKHDHPEQLEHLMYTILTHAENLSTLLLENRPPIKVLIISNFDHALSLTFV 419 EL+ Y + + + HL YT +TH ++L LL+N+P +KVL++SNFD + Sbjct: 361 ELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVA 420 Query: 420 DMLSYYCNNRFIFDIWDELKTSPEILNQTDYDIIVSNFYIPGI-TKKFICRNHLSIMDLV 478 + LSYYC+N F ++W EL+ S E L + YDII+SNF IP I K+ I N+++ + L+ Sbjct: 421 ETLSYYCSNNFELEVWTELELSKESLEDSPYDIIISNFIIPPIENKRLIYSNNINTVSLI 480 Query: 479 NHLNTLSN 486 LN + Sbjct: 481 YLLNAMMF 488
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1531	TONBPROTEIN	51	2e-08	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 51.1 bits (122), Expect = 2e-08 Identities = 26/81 (32%), Positives = 31/81 (38%), Gaps = 4/81 (4%) Query: 2882 PAQPTPNVPIPEVPVK-PVPAQPTPNVPTPEVPVQPTPVVPTPEVPVKPVPAVPEQP--- 2937 +P V P PV P P P E PV P P+ KPV V EQP Sbjct: 54 DLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRD 113 Query: 2938 VVPTPAQPATPVNANPVVSTT 2958 V P ++PA+P T Sbjct: 114 VKPVESRPASPFENTAPARLT 134 Score = 41.1 bits (96), Expect = 3e-05 Identities = 22/78 (28%), Positives = 26/78 (33%), Gaps = 1/78 (1%) Query: 2884 QPTPNVPIPEVPVKPVPAQPTPNVPTPEVP-VQPTPVVPTPEVPVKPVPAVPEQPVVPTP 2942 P P PI V P +P V P P V+P P P K P V E+P Sbjct: 38 LPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPK 97 Query: 2943 AQPATPVNANPVVSTTVK 2960 +P VK Sbjct: 98 PKPKPVKKVQEQPKRDVK 115 Score = 39.6 bits (92), Expect = 1e-04 Identities = 26/100 (26%), Positives = 31/100 (31%), Gaps = 9/100 (9%) Query: 2868 PEVPEVPRLDIPTVPAQPTPNVPIPEVPV---KPVPAQPTPNVPTPEVPVQPTPVVPTPE 2924 V P + P P E PV KP P P +V QP V Sbjct: 59 QAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVK--- 115 Query: 2925 VPVKPVPAVPEQPVVPTPAQPATPVNA--NPVVSTTVKEN 2962 PV+ PA P + P +T A PV S Sbjct: 116 -PVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPR 154 Score = 33.8 bits (77), Expect = 0.007 Identities = 16/52 (30%), Positives = 19/52 (36%), Gaps = 1/52 (1%) Query: 2893 EVPVKPVPAQPTPNVPTPEVPVQPTPVVPTPEVPVK-PVPAVPEQPVVPTPA 2943 +V P PAQP ++P V P PV P P P P A Sbjct: 34 QVIELPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEA 85 Score = 32.7 bits (74), Expect = 0.016 Identities = 30/135 (22%), Positives = 38/135 (28%), Gaps = 17/135 (12%) Query: 2811 VTPSNDKPVPPTPNMPEGPKFAMPEPPVHELPEFNGGVPGMPEVHELPEFNSGVPGMPEV 2870 VTP++ +P PE PEP P P V E P+ P Sbjct: 50 VTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPP-----KEAPVVIEKPKPKPKPKPKPV- 103 Query: 2871 PEVPRLDIPTVPAQPTPNV-PIPEVPVKPVPAQPTPNVPTPEVPVQPTPVVPTPEVPVKP 2929 V QP +V P+ P P + + P V P Sbjct: 104 --------KKVQEQPKRDVKPVESRPASPFENTAPARLTSS--TATAATSKPVTSVASGP 153 Query: 2930 VPAVPEQPVVPTPAQ 2944 QP P AQ Sbjct: 154 RALSRNQPQYPARAQ 168
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1529	TCRTETA	34	0.001	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 34.0 bits (78), Expect = 0.001 Identities = 48/313 (15%), Positives = 106/313 (33%), Gaps = 14/313 (4%) Query: 43 GLLESIFHTTSLLCEIPSGMLADRYSYKTNLYLSRIAGIVSSILMLAGQGNFWIYALAMA 102 G+L +++ C G L+DR+ + L +S V +M W+ + Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATA-PFLWVLYIGRI 104 Query: 103 VSALSYNFDSGTSAAMVYDSAVEAGLKERYLSISSFLSGVSEGTQSLGTVLAGFFVHGQL 162 V+ ++ +G A + + R+ F+S G VL G Sbjct: 105 VAGITGA--TGAVAGAYIADITDGDERARHF---GFMSACFGFGMVAGPVLGGLMGGFSP 159 Query: 163 HLTYYIMIATSIIVLFLIWMLKEPSVKVEKADSVTMKQIMWTVKDELKRN-----PMLFN 217 H ++ A + + L S K E+ + + + R ++ Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPESHKGER-RPLRREALNPLASFRWARGMTVVAALMAV 218 Query: 218 WMILSQIVGVLMCMFYFYYQNQLPDLSGWQISAVMLLGSLLNIVA-VYLASKIGKNYAAL 276 + I+ + V ++ + +++ I + +L+ +A + + Sbjct: 219 FFIMQLVGQVPAALWVIFGEDRF-HWDATTIGISLAAFGILHSLAQAMITGPVAARLGER 277 Query: 277 RLFPILVLLTGVTYMLSYFGTPLIYILIYLISNALHALFQPIFDNDLQGRLPSEVRATML 336 R + ++ G Y+L F T ++ A + P L ++ E + + Sbjct: 278 RALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQ 337 Query: 337 SVYSMMFSLSMIV 349 + + SL+ IV Sbjct: 338 GSLAALTSLTSIV 350
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1527	FLGPRINGFLGI	29	0.027	Flagellar P-ring protein signature.
>FLGPRINGFLGI#Flagellar P-ring protein signature. Length = 373 Score = 29.1 bits (65), Expect = 0.027 Identities = 8/21 (38%), Positives = 10/21 (47%) Query: 31 DILSLTLGEPDFTTPKNIQDA 51 L L L PDF+T + D Sbjct: 191 VNLVLQLRNPDFSTAVRVADV 211

smi_1479

smi_1457

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1479	-2	15	3.309143	response regulator
smi_1478	-2	16	3.299106	histidine kinase
smi_1477	-2	21	3.516994	hypothetical protein
smi_1476	-1	22	3.716560	zinc metalloprotease
smi_1475	-1	20	2.033424	chorismate binding enzyme para-aminobenzoate
smi_1474	-1	19	1.274010	hypothetical protein
smi_1473	-1	16	-1.012285	choline binding protein Cbp11
smi_1472	-1	16	-1.645420	glucose kinase
smi_1471	0	20	-4.075066	thymidylate synthase
smi_1470	0	26	-6.404221	hypothetical protein
smi_1469	2	28	-7.078677	tRNA isopentenylpyrophosphate transferase
smi_1468	1	28	-7.108413	hypothetical protein
smi_1467	3	28	-6.483487	hypothetical protein
smi_1466	2	24	-5.705602	hydrolase
smi_1465	2	21	-5.133764	dehydrogenase
smi_1464	1	18	-4.333784	transcriptional regulator
smi_1463	-1	16	-4.217960	hypothetical protein
smi_1462	-1	17	-3.108281	hypothetical protein
smi_1461	-3	17	-2.814981	choline binding protein Cbp10
smi_1460	-3	17	-3.628577	hypothetical protein
smi_1459	-2	16	-2.854791	hypothetical protein
smi_1458	1	14	-0.817238	hypothetical protein
smi_1457	2	19	0.086535	ATPases of the AAA+ class

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1478	PF03309	35	3e-04	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 35.1 bits (81), Expect = 3e-04 Identities = 25/126 (19%), Positives = 46/126 (36%), Gaps = 14/126 (11%) Query: 5 IIGIDLGGTSIKFAILTTAGEIQE---KWSIKTNILDEGSHIVDDMIESIQHRLDLLGVA 61 ++ ID+ T +++ +G+ + +W I+T D++ +I L+G Sbjct: 2 LLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTA----DELALTI---DGLIGDD 54 Query: 62 AADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKEKIEKALGIPFFIDNDANVAALG 121 A G S V V W + + + GIP +DN V A Sbjct: 55 AERLTGASGLS--TVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGA-- 110 Query: 122 ERWMGA 127 +R + Sbjct: 111 DRIVNC 116
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1471	DHBDHDRGNASE	82	1e-20	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 81.6 bits (201), Expect = 1e-20 Identities = 50/182 (27%), Positives = 88/182 (48%), Gaps = 6/182 (3%) Query: 4 ILITGASGGLAQEMVKLLPND--QLILLGRNKEKLAQLYGKHP----HAEWIEIDITDDS 57 ITGA+ G+ + + + L + + + N EKL ++ HAE D+ D + Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 58 ALETLVADLYLRYGKIDVLINNAGYGIFEEFDQISDQDIHQMFEVNTFALMNLSRCLAAR 117 A++ + A + G ID+L+N AG +SD++ F VN+ + N SR ++ Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130 Query: 118 MKESRKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRLELMPHGVYVTTVNPGPI 177 M + R G I+ + S + + Y+++K AA+ F+ L LEL + + V+PG Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGST 190 Query: 178 RT 179 T Sbjct: 191 ET 192
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1463	HTHFIS	36	8e-04	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 35.6 bits (82), Expect = 8e-04 Identities = 33/127 (25%), Positives = 45/127 (35%), Gaps = 28/127 (22%) Query: 391 LNNVKKEVQKLLRTVEFNQKRLSEGLPIQEQ----------SLHSVFTGNPGTGKTTVAR 440 L K+ KL + + +QE L + TG GTGK VAR Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVAR 178 Query: 441 LLGR---------VLFDRGVLPGDEFKFIEVSESDLIATHIGE--TAVQTQA-ILEKAKG 488 L V + +P D + ES+L G A E+A+G Sbjct: 179 ALHDYGKRRNGPFVAINMAAIPRD------LIESELFGHEKGAFTGAQTRSTGRFEQAEG 232 Query: 489 GILFIDE 495 G LF+DE Sbjct: 233 GTLFLDE 239 Score = 32.5 bits (74), Expect = 0.008 Identities = 28/155 (18%), Positives = 55/155 (35%), Gaps = 45/155 (29%) Query: 638 DIDDVL------LQGTQQPEENQKDALEQLQNLIG----IEKVKKQVEQFISLAELNKKR 687 D+ +++ L ++ +D + L+G ++++ + + + Sbjct: 107 DLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLAR----------- 155 Query: 688 EEQGAAVSEFSLHSLFLGNPGTGKTTVARIVGKILYQKGIIPQNKFIEVS---------R 738 + + L + G GTGK VAR L+ G F+ ++ Sbjct: 156 ------LMQTDLTLMITGESGTGKELVAR----ALHDYGKRRNGPFVAINMAAIPRDLIE 205 Query: 739 SDLVAGYVG---QTAIKTQE-VLKSALGGVLFIDE 769 S+L G+ A + A GG LF+DE Sbjct: 206 SELF-GHEKGAFTGAQTRSTGRFEQAEGGTLFLDE 239
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1458	PF06580	33	0.002	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 33.3 bits (76), Expect = 0.002 Identities = 23/90 (25%), Positives = 42/90 (46%), Gaps = 16/90 (17%) Query: 435 LVEELATNAVKYA-----SGGRISLKIDVHDDAIFIISENN----CLQTEKSLGYGLKNM 485 LVE N +K+ GG+I LK + + + EN T++S G GL+N+ Sbjct: 263 LVE----NGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQNV 318 Query: 486 SNKISVLGG---QMQIFENNKRFRVEITLP 512 ++ +L G Q+++ E + + +P Sbjct: 319 RERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1457	HTHFIS	48	9e-09	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 47.5 bits (113), Expect = 9e-09 Identities = 25/118 (21%), Positives = 47/118 (39%), Gaps = 6/118 (5%) Query: 2 KILLIDDHKLFSQSIKMILELSENIKKVQLVDNFST-ISEIAFNDYDIILIDINLTSLYQ 60 IL+ DD + L + V++ N +T IA D D+++ D+ + Sbjct: 5 TILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMP---D 59 Query: 61 TDGLTLAQEIIDNGCSSKVVILTGYSKKMYEHRAKVMGVYGFLDKSMDPDELVKKLEK 118 + L I V++++ + M +A G Y +L K D EL+ + + Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117

smi_1447

smi_1437

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1447	3	17	-1.643935	membrane GTPase TypA
smi_1446	3	20	-3.506396	transposase, IS1167
smi_1445	4	26	-6.093241	hypothetical protein
smi_1444	2	23	-6.264924	ABC transporter ATP-binding protein
smi_1443	2	20	-5.288748	MurD D-glutamic acid adding enzyme
smi_1442	2	17	-3.863414	MurG UDP-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc
smi_1441	1	18	-0.968917	cell division protein DivIB
smi_1440	-1	16	1.304566	orotidine-5'-phosphate decarboxylase
smi_1439	-1	19	2.790052	orotate phosphoribosyltransferase
smi_1438	-2	17	3.040697	hypothetical protein
smi_1437	-2	14	3.203147	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1447	IGASERPTASE	35	5e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 35.0 bits (80), Expect = 5e-04 Identities = 14/53 (26%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Query: 351 ADKLIMEAEEKAKQEAKEAEKKQEEERKRLEEEKKKQEEESNRNQTSQRSSRR 403 + E +E E KE ++EE+ ++E E K QE +Q S + + Sbjct: 1085 VAQSGSETKETQTTETKETATVEKEEKAKVETE-KTQEVPKVTSQVSPKQEQS 1136 Score = 35.0 bits (80), Expect = 6e-04 Identities = 20/120 (16%), Positives = 46/120 (38%), Gaps = 5/120 (4%) Query: 2 SKDKKNEGKEILEEFKELSEWQKRNQEYLKKKAE-EEAALAEEKEKERQARMASKSEESD 60 + + + +E+ +E K + + E + +E +E E KE + E++ Sbjct: 1058 ATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETE 1117 Query: 61 ETGDRESESNPEDPESAKGESEEKVESSEGDKEEEEIEESGSKEKEEQDKNLAKKEKATK 120 +T + ++ P+ + E+ + + + E KE + Q A E+ K Sbjct: 1118 KTQEVPKVTSQVSPKQEQSETVQP----QAEPARENDPTVNIKEPQSQTNTTADTEQPAK 1173
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1438	PF05272	33	0.001	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 32.7 bits (74), Expect = 0.001 Identities = 15/57 (26%), Positives = 22/57 (38%), Gaps = 6/57 (10%) Query: 30 KGEVVVIL-GPSGCGKSTLLRCLNGLESIQGGDILLYGQSIVENKKDFHLVRQKIGM 85 K + V+L G G GKSTL+ L GL+ I K + + + Sbjct: 594 KFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHF-----DIGTGKDSYEQIAGIVAY 645

smi_1342

smi_1306

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1342	2	17	-3.273359	transcriptional regulator
smi_1341	1	25	-6.529954	choline-binding protein Cbp9
smi_1340	3	30	-5.550120	glycerol uptake facilitator
smi_1339	2	30	-5.250257	hypothetical protein
smi_1338	1	26	-5.687656	hypothetical protein
smi_1337	2	25	-4.211988	hypothetical protein
smi_1336	1	25	-3.381216	transcriptional regulator
smi_1335	1	25	-3.239257	GMP-synthase
smi_1334	1	25	-3.188345	site-specific recombinase, phage integrase
smi_1333	1	27	-3.291936	hypothetical protein
smi_1332	1	27	-2.921303	polyribonucleotide nucleotidyltransferase
smi_1331	0	26	-4.349071	hypothetical protein
smi_1330	1	26	-5.360203	hypothetical protein
smi_1329	1	25	-5.946201	transcriptional regulator, Tn916
smi_1328	0	24	-5.267512	hypothetical protein
smi_1327	0	22	-4.320340	tetracycline resistance protein TetM
smi_1326	0	22	-4.092280	hypothetical protein
smi_1325	1	23	-2.795174	cell wall hydrolase, lytic transglycosylase
smi_1324	1	23	-5.491881	transmembrane amino acid transporter protein
smi_1323	0	26	-7.584042	ATP/GTP-binding protein
smi_1322	1	27	-7.307942	hypothetical protein
smi_1321	1	24	-6.062223	hypothetical protein
smi_1320	1	25	-6.315676	hypothetical protein
smi_1319	0	24	-6.273675	hypothetical protein
smi_1318	0	22	-4.710326	hypothetical protein
smi_1317	2	18	-2.651348	transcriptional regulator, Tn916
smi_1316	1	19	-0.018441	hypothetical protein
smi_1315	1	22	-1.052314	hypothetical protein
smi_1314	1	24	0.359088	hypothetical protein
smi_1313	2	25	1.214939	hypothetical protein
smi_1312	2	23	1.615374	hypothetical protein
smi_1311	4	27	-0.363697	hypothetical protein
smi_1310	5	26	-1.130941	hypothetical protein
smi_1309	5	25	-0.991858	peptidase
smi_1308	4	22	-0.960358	proton-translocating ATPase, F0 sector, subunit
smi_1307	4	22	-1.481720	proton-translocating ATPase, F0 sector, subunit
smi_1306	3	21	-1.605186	proton-translocating ATPase, F0 sector, subunit

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1334	TCRTETOQM	1101	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 1101 bits (2849), Expect = 0.0 Identities = 603/639 (94%), Positives = 622/639 (97%) Query: 1 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI Sbjct: 1 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60 Query: 61 TSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMG 120 TSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMG Sbjct: 61 TSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMG 120 Query: 121 IPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNVCVTNFTESEQWDTVIE 180 IPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPN+CVTNFTESEQWDTVIE Sbjct: 121 IPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIE 180 Query: 181 GNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYS 240 GNDDLLEKYMSGKSLEALELEQEESIRF NCSLFP+YHGSAK+NIGIDNLIEVITNKFYS Sbjct: 181 GNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYS 240 Query: 241 STHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSING 300 STHRG SELCG VFKIEY++KRQRLAYIRLYSGVLHLRDSVR+SEKEKIK+TEMYTSING Sbjct: 241 STHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSING 300 Query: 301 ELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREM 360 ELCKID+AYSGEIVILQNEFLKLNSVLGDTKLLPQR++IENP PLLQTTVEPSKP+QREM Sbjct: 301 ELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREM 360 Query: 361 LLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIY 420 LLDALLEISDSDPLLRYYVDS THEIILSFLGKVQMEV ALLQEKYHVEIE+KEPTVIY Sbjct: 361 LLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIY 420 Query: 421 MERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEG 480 MERPLK AEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEG Sbjct: 421 MERPLKKAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEG 480 Query: 481 IRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQAFRKAGTELLEPYL 540 IRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQ +KAGTELLEPYL Sbjct: 481 IRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYL 540 Query: 541 SFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGL 600 SFK+YAPQEYLSRAY DAPKYCANIV+TQLKNNEVI+ GEIPARCIQ+YR+DLTFFTNG Sbjct: 541 SFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGR 600 Query: 601 SVCLAELKGYQVTTGEPVCQTRRLNSQIDKVRYMFNKIT 639 SVCL ELKGY VTTGEPVCQ RR NS+IDKVRYMFNKIT Sbjct: 601 SVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT 639
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1306	GPOSANCHOR	46	2e-06	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 45.8 bits (108), Expect = 2e-06 Identities = 63/351 (17%), Positives = 116/351 (33%), Gaps = 15/351 (4%) Query: 1084 ALTAKNQEIDDRKDLTQAEKDAAKAEAKKLADAELTKVNAQPDNAETAEAAAAAQKLVND 1143 + + +AEK A +A +L + L A+K Sbjct: 166 GAMNFSTADSAKIKTLEAEKAALEARQAEL-EKALEGAMNFSTADSAKIKTLEAEKAALA 224 Query: 1144 AEDKGVADVTSVYPIAKEEAKKAVADELAKKEKELDDRKDLTTEEKAAAKKEAKDLAKKA 1203 A + E + + K K L+ K +A +K + + Sbjct: 225 ARKADLE--------KALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFS 276 Query: 1204 TDAINAQPAIADTSDKATAAQQAVDTAKTTGVAEVKAVNPEAVKKNVAKKAIE---DALT 1260 T + A + ++ A +++ + AKK +E L Sbjct: 277 TADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLE 336 Query: 1261 AKNNAIDA-RDDLTPEQKTAAKEAAKAKADAAKDAIDKATTDADVDQAKTDGETAVANVT 1319 +N +A R L + + + + +A+ K ++A + D + + Sbjct: 337 EQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKK 396 Query: 1320 PVAK-KPAKQAIAKALEDKNAEIDKRTDLTEEEKATAKKEAKDKADAQLAEIAKQ-PDVA 1377 V K + ALE N E+++ LTE+EKA + + + +A A ++AKQ ++A Sbjct: 397 QVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQAEELA 456 Query: 1378 DTPEAAQTAQTAVDAAKKTGVDEVTAVNPTAVTKPEAKKAIDAKLAEQLKT 1428 + DA P A TKP KA + QL + Sbjct: 457 KLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPS 507 Score = 41.2 bits (96), Expect = 5e-05 Identities = 83/458 (18%), Positives = 162/458 (35%), Gaps = 49/458 (10%) Query: 1073 AKQNAKDAIGNALTAKNQEIDDRKDLTQAEKDAAKAEAKKLADAELTKVNAQPDNAETAE 1132 + +L+ K +I + + + A +A + A T +A+ E + Sbjct: 96 NAKEKLRKNDKSLSEKASKIQELEA-----RKADLEKALEGAMNFSTADSAKIKTLEAEK 150 Query: 1133 AAAAAQKLVNDAEDKGVADVTSVYPIAKEEAKKAVADELAKKEKELDDRKDLTTEEKAAA 1192 AA AA+K + +G + + A A L ++ L+ R+ + A Sbjct: 151 AALAARKADLEKALEGAMNFS--------TADSAKIKTLEAEKAALEARQAELEKALEGA 202 Query: 1193 KKEAKDLAKKATDAINAQPAIADTSDKATAAQQAVDTAKTTGVAEVKAVNPEAVKKNVAK 1252 + + K + A+A A + T A++K + E + Sbjct: 203 MNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQ 262 Query: 1253 KAIEDALTAKNNAIDARDDLTPEQKTAAKEAAKAKADAAKDAIDKATTDADVDQAKTDGE 1312 +E AL N A + KT E A +A+ A +A+ + D + Sbjct: 263 AELEKALEGAMNFSTADSA---KIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLD 319 Query: 1313 TAVANVTPVAKKPAKQAIAKALEDKNAEIDKRTDLTEEEKATAKKEAKDKADAQLAEIAK 1372 + AKK + K E R L A +EAK + +A+ ++ + Sbjct: 320 ASRE-----AKKQLEAEHQKLEEQNKISEASRQSL--RRDLDASREAKKQLEAEHQKLEE 372 Query: 1373 QPDVADTPEAAQTAQTAVDAAKKTGVDEVTAVNPTAVTKPEAKKAIDAKLAEQLKTIEST 1432 Q +++ + Q+ + +DA++ EAKK ++ L E + + Sbjct: 373 QNKISEA--SRQSLRRDLDASR------------------EAKKQVEKALEEANSKLAAL 412 Query: 1433 PDATDDEKKVAADAAKALAAKAKAEIDKAGTDADVKALEDEAKAEIEKSLPLVEDKPNAR 1492 + + +K L K KAE+ +A +A+ KAL+++ + E+ L K + Sbjct: 413 EKLNKEL-----EESKKLTEKEKAEL-QAKLEAEAKALKEKLAKQAEELAKLRAGKASDS 466 Query: 1493 KAIDEEATAKKAAIDSRTDLTPKAKEDLKAQVDKIAEQ 1530 + D + K + KA + + Q Sbjct: 467 QTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQ 504

smi_1269

smi_1248

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1269	2	16	-0.608690	permease
smi_1268	1	20	-3.098610	Sec-independent protein translocase tatC
smi_1267	2	24	-4.878594	Sec-independent protein translocase tatA
smi_1266	0	33	-7.268945	hypothetical protein
smi_1265	2	35	-8.472738	hypothetical protein
smi_1263	5	39	-10.920528	50S ribosomal protein L31 type B
smi_1262	5	38	-11.789238	hypothetical protein
smi_1261	1	26	-8.729746	flavodoxin
smi_1260	0	19	-5.251309	chorismate mutase/prephenate dehydratase
smi_1259	0	20	-4.752939	CrcB protein
smi_1258	1	18	-3.615846	CrcB protein
smi_1257	-1	16	-0.881615	50S ribosomal protein L19
smi_1256	1	12	-1.569591	*hypothetical protein
smi_1255	0	14	-2.921513	hypothetical protein
smi_1254	-1	17	-3.884528	hypothetical protein
smi_1253	-1	18	-4.636048	site-specific recombinase, phage integrase
smi_1252	-2	17	-4.446842	transcriptional regulator
smi_1251	0	20	-5.498925	hypothetical protein
smi_1250	-1	19	-4.939629	permease
smi_1249	-3	15	-3.346766	hydrolase
smi_1248	-4	14	-3.206823	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1265	FLGMOTORFLIM	26	0.041	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 26.0 bits (57), Expect = 0.041 Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 8/63 (12%) Query: 3 PLIQSLTEGQLR-TDIPSFRPGDTVRVHAKVVE-------GNRERIQIFEGVVIARKGAG 54 ++ + +L DI R GD +R+H V GNR++ GVV + A Sbjct: 260 DVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIGNRKKFLCQPGVVGKKIAAQ 319 Query: 55 ISE 57 I E Sbjct: 320 ILE 322
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1257	TCRTETB	29	0.024	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 29.5 bits (66), Expect = 0.024 Identities = 40/214 (18%), Positives = 91/214 (42%), Gaps = 14/214 (6%) Query: 153 IAGVLMLKFNINSIFFINSASFLLLFLSILISYIPDQEHKENGNDDIKEIFVGFKENFTN 212 +LM + + F S S L +S+L I + ++ + + N Sbjct: 202 KGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVD------PGLGKN 255 Query: 213 IKLRISLLFTIVVNIAMLGFNATIIYYLQDQLKLSNSLVGIVYSIAGFGSLIAVTFLSTF 272 I I +L ++ + GF + + Y ++D +LS + +G V G S+I ++ Sbjct: 256 IPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGI 315 Query: 273 L-NKKDTFILMNISMATIPLVIMVSGIVEN----WIFFGICYSI--LSGLITIASVSITT 325 L +++ ++NI + + + + + + ++ I + + LS T+ S +++ Sbjct: 316 LVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSS 375 Query: 326 IQQQESTEYNIGKILSSSFV-IATIFAPFGGILA 358 +Q+ + + +SF+ T A GG+L+ Sbjct: 376 SLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409

smi_1120

smi_1088

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1120	-3	15	-3.613981	peptide chain release factor I
smi_1119	0	23	-6.225900	protoporphyrinogen oxidase
smi_1118	2	32	-8.702822	hypothetical protein
smi_1117	1	32	-9.309906	hypothetical protein
smi_1116	0	32	-9.299492	serine hydroxymethyltransferase
smi_1115	2	30	-9.379495	hypothetical protein
smi_1114	1	31	-9.589132	hypothetical protein
smi_1113	0	32	-9.183339	hypothetical protein
smi_1112	2	29	-8.317920	23S rRNA (uracil-5-)-methyltransferase RumA
smi_1111	1	25	-6.205421	hypothetical protein
smi_1110	0	21	-5.348113	hypothetical protein
smi_1109	0	19	-4.061156	hypothetical protein
smi_1108	0	18	-3.004241	hypothetical protein
smi_1107	0	18	-3.206193	hypothetical protein
smi_1106	0	21	-3.517255	hypothetical protein
smi_1105	-1	21	-4.903908	hypothetical protein
smi_1104	0	25	-6.071906	aminoglycoside 6'-N-acetyltransferase
smi_1103	1	34	-8.822737	hypothetical protein
smi_1102	0	34	-9.536990	hypothetical protein
smi_1101	-1	33	-9.562776	hypothetical protein
smi_1100	-1	34	-11.105468	hypothetical protein
smi_1099	-1	34	-11.119460	hydrolase
smi_1098	-1	35	-11.348625	hypothetical protein
smi_1097	-1	32	-9.478299	neopullulanase
smi_1096	-1	32	-9.045788	hypothetical protein
smi_1095	0	31	-9.007378	transcriptional regulator
smi_1094	1	29	-8.043716	hypothetical protein
smi_1093	2	28	-7.698883	hypothetical protein
smi_1092	2	22	-6.175238	hypothetical protein
smi_1091	2	19	-6.132692	hypothetical protein
smi_1090	1	15	-5.109094	hypothetical protein
smi_1089	1	18	0.381750	hypothetical protein
smi_1088	2	22	0.545161	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1120	THERMOLYSIN	28	0.043	Thermolysin metalloprotease (M4) family signature.
>THERMOLYSIN#Thermolysin metalloprotease (M4) family signature. Length = 544 Score = 28.4 bits (63), Expect = 0.043 Identities = 23/180 (12%), Positives = 51/180 (28%), Gaps = 14/180 (7%) Query: 1 MRKKLFLTSAAVLWAVTAVNSVHAATDVQKVIDETYVQPEYVLGSSLSEDQ--------- 51 M K+ L + + + + A +A V +E + P +V GS L Sbjct: 1 MNKRAMLGAIGLAFGLMAWPFGASAKGKSMVWNEQWKTPSFVSGSLLGRCSQELVYRYLD 60 Query: 52 KNQTLKKLGYNASTDTKELKTMTPDIYSKIMNVANDSS-LQLYSSAKIQKLGDKSPLEVK 110 + + +LG A + ++ +M + + + + D + Sbjct: 61 QEKNTFQLGGQARERLSLIGNKLDELGHTVMRFEQAIAASLCMGAVLVAHVNDGELSSLS 120 Query: 111 IETPENIT----KVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSLEANGAK 166 N+ K + A + + V P E + + + Sbjct: 121 GTLIPNLDKRTLKTEAAISIQQAEMIAKQDVADRVTKERPAAEEGKPTRLVIYPDEETPR 180
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1112	V8PROTEASE	34	0.001	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 33.8 bits (77), Expect = 0.001 Identities = 7/63 (11%), Positives = 19/63 (30%), Gaps = 2/63 (3%) Query: 38 DEEKSNFGNIGKSFSYGKFNLIGLGDYQKV--WVIDKNTVSAKDNFLPIARIINYDLNIF 95 +E+ + G + K + + V + DK + ++ I + + Sbjct: 170 NEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYD 229 Query: 96 TYI 98 Sbjct: 230 LST 232
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1111	SACTRNSFRASE	38	3e-06	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 38.0 bits (88), Expect = 3e-06 Identities = 24/111 (21%), Positives = 37/111 (33%), Gaps = 10/111 (9%) Query: 20 PQLTDKEAIDEVKRYTNGKNTAIFTEVENDTIVG-LALCSLRFDYVEGCKYSPVGFLEGI 78 P E D Y + A F + +G + + S Y +E I Sbjct: 45 PYFKQYEDDDMDVSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYA---------LIEDI 95 Query: 79 IVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTLTNTNSIRFHLNIGF 129 V ++YR K + L K EWAK + N ++ F+ F Sbjct: 96 AVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1100	PF05211	32	0.002	Neuraminyllactose-binding hemagglutinin
>PF05211#Neuraminyllactose-binding hemagglutinin Length = 260 Score = 32.3 bits (73), Expect = 0.002 Identities = 23/108 (21%), Positives = 44/108 (40%), Gaps = 4/108 (3%) Query: 51 TEKDDKTALHLNFYPQQFVGDIKNADVIILAKNPGYSDEYEKLYNNNKDYQQTCLDSLQ- 109 + ++ AL LN++P + +++L YSD K Y N ++ ++ Sbjct: 32 IIETNEVALKLNYHPASEKVQALDEKILLLRPAFQYSDNIAKEYENK--FKNQTTLKVEQ 89 Query: 110 -LKKVGFHAFELDEGDKLGFTAAKFKFWFEDAGMGDLFKTNDDYKKHV 156 L+ G+ +D DK F+ A+ K + M D K+ + Sbjct: 90 ILQNQGYKVINVDSSDKDDFSFAQKKEGYLAVAMNGEIVLRPDPKRTI 137

smi_1046

smi_1028

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1046	2	34	0.578459	hypothetical protein
smi_1045	1	31	-0.605813	dihydroxyacetone kinase
smi_1044	1	28	-0.725685	dihydroxyacetone kinase
smi_1043	0	28	-0.776146	transcriptional regulator
smi_1042	-3	23	-1.507439	dihydroxyacetone kinase
smi_1041	-3	18	-2.612899	hypothetical protein
smi_1040	-2	18	-3.278328	hypothetical protein
smi_1039	-2	18	-3.282259	phosphoenolpyruvate-protein phosphotransferase,
smi_1038	-3	18	-4.169488	phosphocarrier protein HPr
smi_1037	1	25	-5.775747	glutaredoxin-like protein
smi_1036	1	25	-5.902393	ribonucleoside-diphosphate reductase 2, alpha
smi_1035	-1	25	-5.363419	ribonucleoside-diphosphate reductase 2, beta
smi_1034	-2	21	-3.053667	s system repressor
smi_1033	-1	22	-1.965965	hypothetical protein
smi_1032	1	29	-1.296817	6-phospho-beta-galactosidase
smi_1031	3	32	-0.477964	PTS system, lactose-specific IIBC component
smi_1030	2	27	-0.747468	PTS system, lactose-specific IIA component
smi_1029	2	36	-0.400925	transcription antiterminator LacT
smi_1028	2	34	-0.611815	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1046	PHPHTRNFRASE	806	0.0	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 806 bits (2083), Expect = 0.0 Identities = 335/573 (58%), Positives = 443/573 (77%), Gaps = 4/573 (0%) Query: 1 MTEMLKGIAASDGVAVAKAYLLVQPDLSFETISVEDTNAEEARLDVALEASQNELSLIRE 60 M + GIAAS GVA+AKA++ ++P++ E S+ D + E +L ALE S+ EL I++ Sbjct: 1 MHHKITGIAASSGVAIAKAFIHLEPNVDIEKTSITDVSTEIEKLTAALEKSKEELRAIKD 60 Query: 61 KAVGTLGEEAAQVFDAHLMVLSDPELIGQIKETIRAKKVNAEAGLKEVTDMFITIFEGME 120 + ++G + A++F AHL+VL DPEL+ IK I +++NAE LKEV+DMF+++FE M Sbjct: 61 QTEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESM- 119 Query: 121 DNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIVIAHDLTPSDTAQLDKNFVKA 180 DN YM+ERAADIRDV+KRVL +L+G + + A+I EE ++IA DLTPSDTAQL+K FVK Sbjct: 120 DNEYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKG 179 Query: 181 FVTNIGGRTSHSAIMARTLEIAAVLGTNNITEVVKDGDILAVNGITGEVIISPTDEQAAE 240 F T+IGGRTSHSAIM+R+LEI AV+GT +TE ++ GD++ V+GI G VI++PT+E+ Sbjct: 180 FATDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKA 239 Query: 241 FKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTE 300 ++ A+ KQK EWA L + T DG H ELAANIGTPKDV+GV NG E +GLYRTE Sbjct: 240 YEEKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTE 299 Query: 301 FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFR 360 FLYMD PTE+EQ+EAYK V++ M+GKPVV+RT+DIGGDKEL Y +P E+NPFLGFR Sbjct: 300 FLYMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFR 359 Query: 361 ALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLKEFRAAKAVYEEEKANLLAE 420 A+R+ + + +FRTQ+RALLRAS +G L++MFPM+A L+E R AKA+ +EEK LL+E Sbjct: 360 AIRLCLEKQD--IFRTQLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSE 417 Query: 421 GVAVADDIQVGIMIEIPAAAMLADQFAKEVDFMSIGTNDLIQYSMAADRMNEQVSYLYQP 480 GV V+D I+VGIM+EIP+ A+ A+ FAKEVDF SIGTNDLIQY+MAADRMNE+VSYLYQP Sbjct: 418 GVDVSDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQP 477 Query: 481 YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQKAVPLLVGMGLDEFSMSATSVLRTRSL 540 Y+P+ILRL++ VIKAAH+EGKW GMCGEMAGD+ A+PLL+G+GLDEFSMSATS+L RS Sbjct: 478 YHPAILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQ 537 Query: 541 MKKLDTAKMEEYANRALTECSTMEEVLELQKEY 573 + KL +++ +A +AL T EEV +L K+ Sbjct: 538 LLKLSKEELKPFAQKALM-LDTAEEVEQLVKKT 569
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1031	FERRIBNDNGPP	29	0.007	Ferrichrome-binding periplasmic protein signature.
>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature. Length = 296 Score = 28.8 bits (64), Expect = 0.007 Identities = 13/41 (31%), Positives = 17/41 (41%) Query: 33 NSTQGSADFVDSTVNAITEFRKYKQARAILFDKFGHSFMPA 73 N+ QG +F ST +I YK + FD M A Sbjct: 208 NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDA 248
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1028	BINARYTOXINA	28	0.033	Clostridial binary toxin A signature.
>BINARYTOXINA#Clostridial binary toxin A signature. Length = 454 Score = 28.5 bits (63), Expect = 0.033 Identities = 20/57 (35%), Positives = 27/57 (47%), Gaps = 5/57 (8%) Query: 171 VLESPHKPTVIKPNNEELSQLLGREVS-EDLDELKEVLQEPLFAGIEWIIVSLGANG 226 ESP K N+E+ E+S E +ELKE +Q+ LF + VSL G Sbjct: 134 YFESPEKFAF----NKEIRTENQNEISLEKFNELKETIQDKLFKQDGFKDVSLYEPG 186

smi_1010

smi_1002

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1010	4	25	0.424922	hypothetical protein
smi_1009	4	29	0.563716	farnesyl diphosphate synthase
smi_1008	0	19	-0.220416	exodeoxyribonuclease VII, small subunit
smi_1007	4	29	-1.135161	exodeoxyribonuclease VII, large subunit
smi_1006	4	29	-1.460073	Uridine kinase
smi_1005	3	28	-1.586088	tRNA pseudouridine synthase B
smi_1004	3	26	-1.670179	hypothetical protein
smi_1003	3	25	-1.596494	O-acetylhomoserine sulfhydrylase
smi_1002	5	31	-1.540100	formate/nitrate transporter

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1002	GPOSANCHOR	57	2e-09	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 56.6 bits (136), Expect = 2e-09 Identities = 39/242 (16%), Positives = 76/242 (31%), Gaps = 14/242 (5%) Query: 19 QRFSIRKYHFGAASVLLGTALILG--AAQTTAKAEETATENKTEAVASAPKDDKASENVT 76 + +S+RK G ASV + ++ T + + DK Sbjct: 8 RHYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADKFEIENN 67 Query: 77 NVTTP-ALSATTEAAVVENPTLSDEEVAKLAAEASKKDDKASETA-TTEKTEAADKEKAT 134 + + + A+ ++ EE++ + K D SE A ++ EA + Sbjct: 68 TLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEK 127 Query: 135 LTAPLTDKKADKAVDEKADKKDEKKAENPITATK-----TVLEQLTSEAEVLNTTASNFA 189 + A + K +AE A + LE + + + Sbjct: 128 AL-----EGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLE 182 Query: 190 DKKAEDKAGKEAIATAVASAKIQIEASKKTLAAGEITKDELDAQLQRISSAIEAVYDEMK 249 +KA +A + + A+ A A + E K L A+ + A+E + Sbjct: 183 AEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFST 242 Query: 250 RA 251 Sbjct: 243 AD 244 Score = 39.7 bits (92), Expect = 3e-04 Identities = 34/196 (17%), Positives = 67/196 (34%), Gaps = 7/196 (3%) Query: 40 ILGAAQTTAKAEETATENKTEAVASAPKDDKASENVTNVTTPALSATTEAAVVENPTLSD 99 L A + + N + A ++ K +A + L E A+ + S Sbjct: 152 ALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSA 211 Query: 100 EEVAKLAAEASKKDDKASETATTEKTEAADKEKATLTAPLTDKKADKAVDEKADKKDEKK 159 + A +A+ KA E + L +KA + +K + Sbjct: 212 KIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEG 271 Query: 160 AENPITATKTVLEQLTSEAEVLNTTASNFADKKAEDKAGKEAIATAVASAKIQIEASKKT 219 A N TA ++ L +E L +KA+ + + + S + ++AS++ Sbjct: 272 AMNFSTADSAKIKTLEAEKAALEA-------EKADLEHQSQVLNANRQSLRRDLDASREA 324 Query: 220 LAAGEITKDELDAQLQ 235 E +L+ Q + Sbjct: 325 KKQLEAEHQKLEEQNK 340 Score = 38.9 bits (90), Expect = 5e-04 Identities = 30/195 (15%), Positives = 55/195 (28%), Gaps = 5/195 (2%) Query: 45 QTTAKAEETATENKTEAVASAPKDDKASENVTNVTTPALSATTEAAVVENPTLSDEEVAK 104 +AK + E A A +KA E N +T + + + + + Sbjct: 138 ADSAKIKTLEAEKAALAARKA-DLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELE 196 Query: 105 LAAEASKKDDKASETATTEKTEAADKEKATLTAPLTDKKADKAVDEKADKKDEKKAENPI 164 A E + A A + K + E Sbjct: 197 KALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTL-EAEK 255 Query: 165 TATKTVLEQLTSEAEVLNTTASNFADKKAEDKAGKEAIATAVASAKIQI---EASKKTLA 221 A + +L E ++ + K +A K A+ A + Q A++++L Sbjct: 256 AALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLR 315 Query: 222 AGEITKDELDAQLQR 236 E QL+ Sbjct: 316 RDLDASREAKKQLEA 330

smi_0984

smi_0979

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0984	4	21	-0.718091	DNA primase
smi_0983	5	21	-0.355852	ABC transporter ATP-binding protein
smi_0982	4	20	0.309734	ABC transporter, membrane-spanning permease
smi_0981	2	15	0.699328	ABC transporter substrate-binding protein
smi_0980	3	18	0.645930	T-box leader
smi_0979	3	18	0.838604	transposase, ISSmi1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0984	VACCYTOTOXIN	31	0.009	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 31.2 bits (70), Expect = 0.009 Identities = 24/100 (24%), Positives = 46/100 (46%), Gaps = 15/100 (15%) Query: 257 ANSYFAMVNGG----WFGLGLGNSIEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALVF 312 +N A+ NG F +E R Y + ++ + V++EF G+S ++L Sbjct: 1177 SNQKVALKNGASSQHLFNASAN--VEARYYYGDTSYFYMNAGVLQEFANFGSSNAVSLNT 1234 Query: 313 FLILRIILVGIRAKNPFNSMMAIGVGGMMLV--QVFVNIG 350 F + +NP N+ + +GG + + +VF+N+G Sbjct: 1235 FKVNA-------TRNPLNTHARVMMGGELKLAKEVFLNLG 1267
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0980	DPTHRIATOXIN	30	0.019	Diphtheria toxin signature.
>DPTHRIATOXIN#Diphtheria toxin signature. Length = 567 Score = 29.7 bits (66), Expect = 0.019 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Query: 59 NESPEHLTNKEVLYQWLKKETEVQLEHP-LPELKQIAD---VFVNGNLA 103 +ESP ++E Q+L++ + LEHP L ELK + VF N A Sbjct: 263 SESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYA 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0979	FLAGELLIN	34	0.003	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 34.2 bits (78), Expect = 0.003 Identities = 57/378 (15%), Positives = 97/378 (25%), Gaps = 20/378 (5%) Query: 693 DAAKQAAQDAATKANQAIDAATDNA--GVATAQT-----DGIAAIEAVTPTVAVKAAA-- 743 DAA QA + T + + A+ NA G++ AQT + I ++V+A Sbjct: 43 DAAGQAIANRFTSNIKGLTQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGT 102 Query: 744 ---------KAEVAKKLAEKLTALEGTPNATKEEKDAAKQAAQDAATKANQAIDAATDNA 794 + E+ ++L E T + Q + I Sbjct: 103 NSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKI 162 Query: 795 GVATAQTDGIAAIEAVTPTV-AVKAAAKAEVAKKLAEKLTALEGTPNATKEEKDAAKQAA 853 V + DG TV +K++ K + Sbjct: 163 DVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPT 222 Query: 854 QDAATKANQAIDAATDNAGVATAQTDGIAAIEAVTPTVAVKAAAKAEVAKKLAEKLTALE 913 N A T + D ++ T KA A A K + Sbjct: 223 VPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKG 282 Query: 914 GTPNATKEEKDAAKQAAQDAATKANQAIDAATDNAGVATAQTDGIAAIEAVTPTVAVKAA 973 T + + + A AG A + + + V +V Sbjct: 283 VTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQF 342 Query: 974 AKAEVAKKLAEKLTALEGTPNATKEEKDAAKQAAQDAATKA-NQAIDAATDNAGVATAQT 1032 + K + KL+ LE E K A A + T + Sbjct: 343 TFDDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGV 402 Query: 1033 DGIAAIEAVTPTVAVKAA 1050 + +A + Sbjct: 403 STLINEDAAAAKKSTANP 420

smi_0849

smi_0835

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0849	3	24	-2.713720	4-methyl-5(b-hydroxyethyl)-thiazole
smi_0848	0	23	-3.621852	rod shape determining protein RodA
smi_0847	0	12	-0.066503	ATP-dependent helicase DinG
smi_0846	0	11	1.128540	protease
smi_0845	-1	10	1.163135	hypothetical protein
smi_0844	-1	11	1.123099	histidine kinase
smi_0843	-1	13	1.380259	response regulator
smi_0842	-1	14	1.458731	aminopeptidase N
smi_0841	-1	13	-0.189462	hypothetical protein
smi_0840	-2	16	-3.510256	hypothetical protein
smi_0839	-1	18	-3.309274	hypothetical protein
smi_0838	-2	20	-4.741512	hypothetical protein
smi_0837	-1	23	-7.065851	short chain dehydrogenase
smi_0836	-2	20	-6.282632	hypothetical protein
smi_0835	-2	15	-3.959357	oxidoreductase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0849	UREASE	25	0.043	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 25.5 bits (56), Expect = 0.043 Identities = 10/26 (38%), Positives = 13/26 (50%) Query: 13 INPSIGDEIDAWAFGVEPDLLADLVL 38 INP+I + +E ADLVL Sbjct: 411 INPAIAHGLSHEIGSLEVGKRADLVL 436
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0845	DHBDHDRGNASE	92	2e-24	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 91.7 bits (227), Expect = 2e-24 Identities = 63/252 (25%), Positives = 105/252 (41%), Gaps = 24/252 (9%) Query: 3 RRVLITGVSSGIGLAQARLFLEKSYQVYGVDQGENPLL-----EGDFHFLQRDLTLDL-- 55 + ITG + GIG A AR + + VD L D+ Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 56 -EPIFDWCPR-------VDVLCNTAGVLDDYKPLLEQTAQEIQEIFEINYMTPVELTRYY 107 I + R +D+L N AGVL + + +E + F +N +R Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLR-PGLIHSLSDEEWEATFSVNSTGVFNASRSV 127 Query: 108 LTQMLENKKGTIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLAIDYAEAGIQIFGIAP 167 M++ + G+I+ + S + + AY SSK A FTK L ++ AE I+ ++P Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 168 GAVKTAMT--------AADFEPGGLADWVASETPIKRWIEPEEVAEVSLFLASGKVSAMQ 219 G+ +T M A+ G + + P+K+ +P ++A+ LFL SG+ + Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHIT 247 Query: 220 GQILTIDGGWSL 231 L +DGG +L Sbjct: 248 MHNLCVDGGATL 259
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0839	RTXTOXIND	44	5e-07	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 44.4 bits (105), Expect = 5e-07 Identities = 27/154 (17%), Positives = 53/154 (34%), Gaps = 8/154 (5%) Query: 47 LVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVKYSSSE 106 +++ G V +G +T + EI+V G+ V +G L+K ++ Sbjct: 72 FILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALG 131 Query: 107 AQAAYDSASRAVAKADRHINELNQARNEAASAPAP--QLPASAV-GEGVAAQAPAPVSGN 163 A+A ++ +A L Q R + S +LP + E Sbjct: 132 AEADTLKTQSSLLQA-----RLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLR 186 Query: 164 SVSSIDAQLGDARDARADAAAQLSKAQSQLDAMT 197 S I Q ++ + L K +++ + Sbjct: 187 LTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVL 220

smi_0815

smi_0771

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0815	3	21	-0.649626	hypothetical protein
smi_0814	5	27	-1.395518	peptidyl-prolyl cis-trans isomerase
smi_0813	6	34	-2.562873	ABC transporter ATP-binding protein
smi_0812	5	33	-3.279120	VanZ-related protein
smi_0811	3	33	-3.465171	23S rRNA (adenine(2503)-C(2))-methyltransferase
smi_0810	1	31	-2.884379	hypothetical protein
smi_0809	1	30	-4.289602	superoxide dismutase
smi_0808	1	34	-5.602535	DNA polymerase III, delta subunit
smi_0807	1	34	-6.262555	dihydroorotate dehydrogenase
smi_0806	1	34	-5.405487	glutamate dehydrogenase
smi_0805	1	35	-6.887442	transposase, ISSsu4
smi_0804	2	37	-9.336770	transposase, ISSsu4, authentic frameshift
smi_0803	1	36	-8.978302	transposase, ISSsu4, authentic frameshift
smi_0802	0	31	-7.107284	hypothetical protein
smi_0801	0	32	-6.871439	transposase, ISSsu4, authentic frameshift
smi_0800	0	29	-6.742910	transposase, ISSsu4, authentic frameshift
smi_0799	2	31	-5.592841	ATP synthase, subunit D
smi_0798	3	30	-5.306820	V-type H+-ATPase, subunit B
smi_0797	3	30	-5.077358	V-type H+-ATPase, subunit A
smi_0796	2	28	-5.889335	V-type H+-ATPase, subunit F
smi_0795	2	27	-5.358423	V-type H+-ATPase, subunit C
smi_0794	1	29	-6.212384	V-type H+-ATPase, subunit E
smi_0793	0	29	-6.696806	V-type H+-ATPase, subunit K
smi_0792	1	28	-5.147210	V-type H+-ATPase, subunit I
smi_0791	4	30	-4.970973	hypothetical protein
smi_0790	4	28	-4.759206	transcriptional regulator
smi_0789	2	28	-4.292278	oxidoreductase, GFO/IDH/MOCA family
smi_0788	1	28	-2.716651	hypothetical protein
smi_0787	2	20	-0.044194	sodium:solute symporter family protein
smi_0786	2	28	2.100305	N-acetylneuraminate lyase
smi_0785	3	34	4.215659	N-acetylmannosamine-6-phosphate epimerase
smi_0784	3	37	4.710840	transcriptional regulator
smi_0783	3	35	4.407620	metal-dependent CAAX amino terminal membrane
smi_0782	1	34	4.045445	hypothetical protein
smi_0781	1	29	3.489720	hypothetical protein
smi_0780	0	24	2.993975	hypothetical protein
smi_0779	-1	20	2.966106	methyl transferase
smi_0778	-1	15	2.480649	hypothetical protein
smi_0777	0	15	2.223458	hypothetical protein
smi_0776	-1	13	0.523113	50S ribosomal protein L7/L12
smi_0775	-2	14	-1.176463	50S ribosomal protein L10
smi_0774	-1	12	-2.115848	transposase, ISSmi1
smi_0773	-1	14	-3.119019	transposase, ISSmi1
smi_0772	1	17	-3.734184	transposase, ISSmi1
smi_0771	0	16	-3.010651	transposase, ISSmi1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0810	IGASERPTASE	34	0.003	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 34.3 bits (78), Expect = 0.003 Identities = 42/285 (14%), Positives = 81/285 (28%), Gaps = 16/285 (5%) Query: 679 PTTATSKDAAKQEITNAAEAKKSAIDGQAGLTSEEKAAAKAAVDTEAEKAKAAIDKATDQ 738 D N A+ + + + +++K + +Q Sbjct: 999 TPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTV--EKNEQ 1056 Query: 739 AGVDSAKAAGKQAIENVPTNGTNATTAKEAAKKAIIDAANKKKAEIDANPALTKEEKEAA 798 ++ + A E N T + A + TKE Sbjct: 1057 DATETTAQNREVAKEAKSNVKANTQTNE---------VAQSGSETKETQTTETKETATVE 1107 Query: 799 KKAVDAEAEKAKQEIDKATDQAGIDKAKNDGLTAIENVPTNGTNATTAKEAAKKAITDAA 858 K+ + QE+ K T Q + +++ + KE + T A Sbjct: 1108 KEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTAD 1167 Query: 859 NKKKA-ATDKATDQAGVDSAKVAGKKVIENVPTTATSKDAAKQEITNAAEAKKSAIDGQA 917 ++ A T +Q +S V + P T A Q N+ + K + Sbjct: 1168 TEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTP--ATTQPTVNSESSNKPKNRHRR 1225 Query: 918 GLTAEEKAAAKAAVDETNSRKVATELPNTGTTDSTVAVLAAVASA 962 + + A + VA L + +T++ + A A A Sbjct: 1226 SVRSVPHNVEPATTSSNDRSTVA--LCDLTSTNTNAVLSDARAKA 1268
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0778	UREASE	37	9e-05	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 37.0 bits (86), Expect = 9e-05 Identities = 23/65 (35%), Positives = 32/65 (49%), Gaps = 9/65 (13%) Query: 219 RTAALLQKMK---------SGDASQFPIETALKALTIEGAKVLGMDEQIGSLEVGKQADF 269 RT KMK +GD F ++ + TI A G+ +IGSLEVGK+AD Sbjct: 375 RTWQTADKMKRQRGRLKEETGDNDNFRVKRYIAKYTINPAIAHGLSHEIGSLEVGKRADL 434 Query: 270 LVIQP 274 ++ P Sbjct: 435 VLWNP 439
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0772	BACINVASINB	28	0.033	Salmonella/Shigella invasin protein B signature.
>BACINVASINB#Salmonella/Shigella invasin protein B signature. Length = 593 Score = 28.2 bits (62), Expect = 0.033 Identities = 15/63 (23%), Positives = 35/63 (55%), Gaps = 4/63 (6%) Query: 87 EDLSDLPDMEELAQMSPDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAE 146 ++LS++ + L M FI+ + K+ + ++D+ +L++ E E++ AE + E Sbjct: 248 DNLSNVARLTMLMAM----FIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEFQEE 303 Query: 147 SKK 149 ++K Sbjct: 304 TRK 306

smi_0760

smi_0733

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0760	0	18	3.879508	glycosyl transferase
smi_0759	-1	18	3.478067	glycosyl transferase
smi_0758	-1	16	2.630077	phosphorylcholine transferase LicD3
smi_0757	2	18	1.240516	transcriptional regulator
smi_0756	2	18	0.737224	prephenate dehydratase
smi_0755	0	18	1.145738	shikimate kinase
smi_0754	-2	16	1.581913	3-phosphoshikimate 1-carboxyvinyltransferase
smi_0753	-2	18	1.756355	hypothetical protein
smi_0752	-2	17	2.487738	hypothetical protein
smi_0751	-2	19	3.215075	prephenate dehydrogenase
smi_0750	-2	22	3.313421	chorismate synthase,
smi_0749	-1	23	3.133977	3-dehydroquinate synthase
smi_0748	0	30	3.083543	shikimate 5-dehydrogenase
smi_0747	-2	27	3.336312	3-dehydroquinase
smi_0746	-2	23	2.450330	hypothetical protein
smi_0745	-1	23	2.966359	phosphoglycerol transferase and related
smi_0744	-1	22	3.184171	hypothetical protein
smi_0743	-2	16	2.723737	ABC transporter ATP-binding protein
smi_0742	-1	15	2.103088	alpha-amylase
smi_0741	0	16	1.922665	alanyl-tRNA synthetase
smi_0740	0	18	2.225096	hypothetical protein
smi_0739	0	18	2.248942	transposase, ISSmi1
smi_0738	-2	18	2.191880	ABC transporter, spermidine/putrescine-binding
smi_0737	-1	22	3.007476	ABC-transporter, spermidine/putrescine transport
smi_0736	0	19	3.669271	ABC-transporter, spermidine/putrescine transport
smi_0735	2	18	3.803411	ABC transporter, ATP-binding
smi_0734	3	17	3.943809	UDP-N-acetylenolpyruvoylglucosamine reductase
smi_0733	2	19	3.224332	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0757	UREASE	29	0.035	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 28.9 bits (65), Expect = 0.035 Identities = 20/83 (24%), Positives = 31/83 (37%), Gaps = 16/83 (19%) Query: 122 IHFV---QIPTSLTAQVDSSIGGKTGVN--------TPFAKNMVGTFAQPDGVLIDPLVL 170 IHF+ QI +L + + +GG TG TP ++ D P+ L Sbjct: 137 IHFICPQQIEEALMSGLTCMLGGGTGPAHGTLATTCTPGPWHIARMIEAADAF---PMNL 193 Query: 171 GTLGK--RELIEGMGEVIKYGLI 191 GK L + E++ G Sbjct: 194 AFAGKGNASLPGALVEMVLGGAT 216
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0746	MYCMG045	51	4e-09	Hypothetical mycoplasma lipoprotein (MG045) signature.
>MYCMG045#Hypothetical mycoplasma lipoprotein (MG045) signature. Length = 483 Score = 50.9 bits (121), Expect = 4e-09 Identities = 78/331 (23%), Positives = 136/331 (41%), Gaps = 65/331 (19%) Query: 25 LDSKINSRDSQKLVIYNWGDYIDPELLEQFTEETGIQVQYETFDSNEAMYTKIKQGGTTY 84 L S ++S S V+ N+ YI P LLE+ E+ + + T+ SNE + TY Sbjct: 16 LSSILSSCGSTTFVLANFESYISPLLLERVQEKH--PLTFLTYPSNEKLINGF--ANNTY 71 Query: 85 DIAIPSEYMINKMKDEDLLVPLDYSK-----------------------IEGLENIGPEF 121 +A+ S Y ++++ + DLL P+D+S+ I+ ++ I + Sbjct: 72 SVAVASTYAVSELIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLFIDSIKEISQQT 131 Query: 122 LNQSFDPGNKFSIPYFWGTLGIVYNETMVEEAPEH---WDDLWKPEYK-------NSIML 171 + + +++PYF L VY + E + W D+ K K N ++ Sbjct: 132 KDSKNNELLHWAVPYFLQNLVFVYRGEKISELEQENVSWTDVIKAIVKHKDRFNDNRLVF 191 Query: 172 FDGAREVLGLG---------------LNSLGYSLNSKDS-QQLEETVDKLYKLTPNIKA- 214 D AR + L + +GY N +S Q+L T L + N + Sbjct: 192 IDDARTIFSLANIVNTNNNSADVNPKEDGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSN 251 Query: 215 IVADEM-----KGYMIQNNAAIGVTFSGEASQMLEKNE----NLRYVVPTEASNLWFDNM 265 IV +E+ +G ++ N A+ G+ L + + N ++V + S + D + Sbjct: 252 IVINELASGRRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLL 311 Query: 266 VIPKTVKN-QDAAYAFINFMLKPENALKNAE 295 VI K N Q A+ I F L + A + E Sbjct: 312 VINKQQSNFQKEAHEII-FDLALDGADQTKE 341
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0738	ADHESNFAMILY	31	0.005	Adhesin family signature.
>ADHESNFAMILY#Adhesin family signature. Length = 309 Score = 31.0 bits (70), Expect = 0.005 Identities = 17/45 (37%), Positives = 21/45 (46%), Gaps = 5/45 (11%) Query: 4 KKWIFVLCSFLATFFLVACQSGSNGSQSAVEAIKQKGKLVVATSP 48 KK +L FL+ LVAC SG + S QK K+V S Sbjct: 2 KKLGTLLVLFLSAIILVACASGKKDTTS-----GQKLKVVATNSI 41

smi_0710

smi_0694

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0710	1	21	-5.051344	hypothetical protein
smi_0709	2	19	-4.698536	prolipoprotein diacylglycerol transferase
smi_0708	2	18	-4.663615	Hpr(Ser) kinase/phosphatase
smi_0707	3	17	-4.561706	30S ribosomal protein S21
smi_0706	3	19	-4.834799	N-acetylglucosamine-6-phosphate isomerase
smi_0705	3	18	-4.651990	S-adenosylmethionine:tRNA
smi_0703	1	22	-1.327614	ribonuclease BN
smi_0702	0	23	-1.936325	Na+/H+ antiporter
smi_0701	2	24	-5.665095	hypothetical protein
smi_0700	0	26	-6.164653	hypothetical protein
smi_0699	-1	15	-3.262285	hypothetical protein
smi_0698	-1	14	-2.631693	hypothetical protein
smi_0697	-2	11	-1.023950	hypothetical protein
smi_0696	-1	10	-0.742847	cell wall-associated serine proteinase
smi_0695	1	14	1.466468	*ABC transporter permease
smi_0694	2	15	1.162095	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0705	SUBTILISIN	93	5e-22	Subtilisin serine protease family (S8) signature.
>SUBTILISIN#Subtilisin serine protease family (S8) signature. Length = 326 Score = 93.0 bits (231), Expect = 5e-22 Identities = 49/233 (21%), Positives = 85/233 (36%), Gaps = 57/233 (24%) Query: 214 LKAINAQF-GKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKGSMRFKKEDLKGTDKNFWL 272 ++ I A GRG+ ++ +DTG D H DLK Sbjct: 26 VEMIQAPAVWNQTRGRGVKVAVLDTGCDADH-----------------PDLKA------- 61 Query: 273 SDKIPHAFNYYNGGKITVEKADDGSDYFDPHGMHIAGILAGNDTEKDIKNFNGIDGIAPN 332 +I N+ + + E D + HG H+AG +A + N NG+ G+AP Sbjct: 62 --RIIGGRNFTDDDEGDPEIFKDY----NGHGTHVAGTIAATE------NENGVVGVAPE 109 Query: 333 AQIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL 392 A + K+ + GSG + I +I+ VD++S+S G +A++ Sbjct: 110 ADLLIIKVLNKQGSGQYDW--IIQGIYYAIEQKVDIISMSLGGPEDVPELH---EAVKKA 164 Query: 393 RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVAS 445 + I ++ A GN T + + + I+V + Sbjct: 165 VASQILVMCAAGNEGDGDDR---------------TDELGYPGCYNEVISVGA 202 Score = 59.9 bits (145), Expect = 3e-11 Identities = 38/139 (27%), Positives = 56/139 (40%), Gaps = 32/139 (23%) Query: 665 PDVSAPGKNIKSTLNVINGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLE 724 D+ APG++I ST+ Y SGTSMATP VA + LI+ ER Sbjct: 219 VDLVAPGEDILSTVP----GGKYATFSGTSMATPHVAGALALIKQLANASFER------- 267 Query: 725 GDDKIDLTSLT-KIALQNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVAT 783 DLT L P+ + SP+ +G GL+ + E+ Sbjct: 268 -----DLTEPELYAQLIKRTIPLGN------------SPKMEGNGLLYLTAV--EELSRI 308 Query: 784 FKNKDSKGLVNSYGSISLK 802 F + G++ S S+ +K Sbjct: 309 FDTQRVAGIL-STASLKVK 326
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0698	TOXICSSTOXIN	25	0.035	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 25.4 bits (55), Expect = 0.035 Identities = 6/25 (24%), Positives = 13/25 (52%) Query: 45 LLNLDIKVRRLLVKNYSVFYRFDKD 69 + LD ++R L + + ++ DK Sbjct: 166 ISTLDFEIRHQLTQIHGLYRSSDKT 190
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0694	PF06580	31	0.009	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 31.0 bits (70), Expect = 0.009 Identities = 22/102 (21%), Positives = 43/102 (42%), Gaps = 15/102 (14%) Query: 288 ILSLSSV--QELRDDREEIDLLQMTQSLVKDYTLLAKKRELQIDNSLTYQ----QAYLNP 341 + SLS + LR L ++V Y LA + ++ L ++ A ++ Sbjct: 197 LTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQ---FEDRLQFENQINPAIMDV 253 Query: 342 SVMKLILSNLISNAIKHSVL----GGLVRIG--EREGELFIE 377 V +++ L+ N IKH + GG + + + G + +E Sbjct: 254 QVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLE 295

smi_0669

smi_0638

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0669	0	14	3.519478	phosphomethylpyrimidine kinase
smi_0668	0	18	4.294587	tRNA pseudouridine synthase A
smi_0667	0	25	4.751553	major facilitator superfamily
smi_0666	0	24	4.421457	hypothetical protein
smi_0665	0	23	4.035315	PhnA protein, required for expression of the
smi_0664	-1	20	3.064741	cytidylate kinase
smi_0663	0	21	3.062048	hypothetical protein
smi_0662	0	19	2.476938	ferredoxin
smi_0661	-2	14	1.655356	glycosyl transferase
smi_0660	0	16	1.423613	UDP-glucose 4-epimerase
smi_0659	-1	16	0.843866	hypothetical protein
smi_0658	0	24	2.981652	hypothetical protein
smi_0657	0	21	1.265585	hypothetical protein
smi_0656	2	20	-1.348647	Bcl-2 family protein
smi_0655	0	14	-1.276810	P-type ATPase-metal cation transport
smi_0654	0	11	-0.823792	transposase, ISSmi1
smi_0653	-1	13	-0.220408	1-acylglycerol-3-phosphate O-acyltransferase
smi_0652	-1	17	-1.381947	esterase of alpha/beta hydrolase superfamily
smi_0651	0	19	0.068352	iron(III) dicitrate-binding lipoprotein
smi_0650	2	25	2.565777	ABC-transporter, Fe3+-siderophore, ATP-binding
smi_0649	3	26	2.859146	ABC-transporter Fe3+-siderophore, permease
smi_0648	4	25	2.860733	30S ribosomal protein S15
smi_0647	1	23	3.754021	hypothetical protein
smi_0646	0	22	3.433048	hypothetical protein
smi_0645	0	24	3.481573	Threonyl-tRNA synthetase, threonine-tRNA ligase
smi_0644	-2	18	3.516139	hypothetical protein
smi_0643	-2	13	1.820450	ABC transporter ATP-binding protein
smi_0642	0	11	-0.051182	histidine kinase
smi_0641	0	18	-1.735400	response regulator
smi_0640	1	17	-1.284393	hypothetical protein
smi_0639	0	18	-2.116779	hypothetical protein
smi_0638	0	23	-3.690473	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0668	NUCEPIMERASE	183	2e-57	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 183 bits (465), Expect = 2e-57 Identities = 87/350 (24%), Positives = 149/350 (42%), Gaps = 48/350 (13%) Query: 4 KILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLEV--VERITGVEIPFYEADI 61 K LVTG AGFIG H L++AGHQVV +DNL + SL+ +E + F++ D+ Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61 Query: 62 RDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNNIAGTVSLLKAMEEANCKNI 121 D + + D+F V AV S P AY D+N+ G +++L+ +++ Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121 Query: 122 IFSSSATVYGDPHTVPILE----DFPLSVTNPYGRTKLMLEEI---LTDIYKADSEWNVV 174 +++SS++VYG +P D P+S Y TK E + + +Y Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVS---LYAATKKANELMAHTYSHLYGLP----AT 174 Query: 175 LLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQVFGDDYDTEDGTGVRDY 234 LR+F G P G P+ L T+ A+ + + + V+ G RD+ Sbjct: 175 GLRFFTVYG----------PWGRPDMALFKFTK-AMLEGKSIDVYN------YGKMKRDF 217 Query: 235 IHVVDLAKGHVAALKKIQKGSG---------------LNVYNLGTGKGYSVLEIIQNMEK 279 ++ D+A+ + I VYN+G +++ IQ +E Sbjct: 218 TYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALED 277 Query: 280 AVGRPIPYRIVDRRPGDIAACYSDPAKAKGELGWEAELGITQMCEDAWRW 329 A+G ++ +PGD+ +D +G+ E + ++ W Sbjct: 278 ALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNW 327
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0659	ADHESNFAMILY	31	0.008	Adhesin family signature.
>ADHESNFAMILY#Adhesin family signature. Length = 309 Score = 30.6 bits (69), Expect = 0.008 Identities = 11/68 (16%), Positives = 25/68 (36%), Gaps = 7/68 (10%) Query: 1 MKKILSILLVTVATLTMAACGNTTTEKATTQSSTETSQKASTETTYPLTVKTYDAKGNEV 60 MKK+ ++L++ ++ + + AC + T + QK T + + Sbjct: 1 MKKLGTLLVLFLSAIILVACASGK-------KDTTSGQKLKVVATNSIIADITKNIAGDK 53 Query: 61 EQVFDKAP 68 + P Sbjct: 54 IDLHSIVP 61
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0649	HTHFIS	79	4e-19	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 78.7 bits (194), Expect = 4e-19 Identities = 31/142 (21%), Positives = 66/142 (46%), Gaps = 3/142 (2%) Query: 3 KILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQSEPHLVLMDIGLPLFNGYH 62 IL+ +DD IR + + LS G++V + + + + LV+ D+ +P N + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 63 WCQEIRKI-SKVPIMFLSSRDQAMDIVMAINMGADDFVTKPFDQQVLLAKVQGLL--RRS 119 I+K +P++ +S+++ M + A GA D++ KPFD L+ + L + Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124 Query: 120 YEFGRDESLLEYAGVILNTKSM 141 ++ + ++ + +M Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAM 146

smi_0599

smi_0586

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0599	-1	18	3.101904	hypothetical protein
smi_0598	-1	20	3.586812	hypothetical protein
smi_0597	0	19	3.804014	ABC transporter-sugar transport,
smi_0596	-1	19	4.215648	ABC transporter permease-sugar transport,
smi_0594	-2	16	3.844625	ABC transporter, sugar transporter, sugar
smi_0593	-2	18	3.008093	N-acetylmannosamine-6-phosphate 2-epimerase 2
smi_0592	-1	19	1.848860	sialidase A (neuraminidase A)
smi_0591	0	20	2.122443	acetyl xylan esterase
smi_0590	1	19	2.274947	hypothetical protein
smi_0589	1	19	2.426495	hypothetical protein
smi_0588	-1	17	2.177011	transposase
smi_0587	-2	16	2.450016	transposase
smi_0586	-2	17	3.059058	branch migration of Holliday junctions,

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0593	ALARACEMASE	354	e-123	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 354 bits (910), Expect = e-123 Identities = 130/365 (35%), Positives = 185/365 (50%), Gaps = 17/365 (4%) Query: 7 RPTKALIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAYGHGAVTVAKAIQDDVDGFCVSN 66 RP +A + L A++QN+ + + W+VVKANAYGHG + AI DGF + N Sbjct: 3 RPIQASLDLQALKQNLSIVRQAATHARV-WSVVKANAYGHGIERIWSAI-GATDGFALLN 60 Query: 67 IDEAIELRQAGLSKKILIL-GVSEIEAVSLAKEYDITLTVAGLEWIQALLDKEADLTGLT 125 ++EAI LR+ G IL+L G + + + ++ +T V ++AL + L Sbjct: 61 LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAP-LD 119 Query: 126 VHLKIDSGMGRIGFREAGEAEQAQDLLQQHGAYVEGIFTHFATADEESDTYFNTQLERFK 185 ++LK++SGM R+GF+ Q L + +HFA A+ + + R + Sbjct: 120 IYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPD--GISGAMARIE 177 Query: 186 TILESMKGLPELVHASNSATTLWHAETIFNAVRMGDAMYGLNPSGEVLDL-PYGLTPALT 244 E GL SNSA TLWH E F+ VR G +YG +PSG+ D+ GL P +T Sbjct: 178 QAAE---GLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMT 234 Query: 245 LESALVHVKTVPVGACMGYGATYQADSEQVIATVPIGYADGWTRDMQN-FSVLVDGQACP 303 L S ++ V+T+ G +GYG Y A EQ I V GYADG+ R VLVDG Sbjct: 235 LSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTM 294 Query: 304 IVGRVSMDQITIRLPKL--YPLGTKVTLIGSNGDKEITATQVATYRGTINYEVVCLLSDR 361 VG VSMD + + L +GT V L G KEI VA GT+ YE++C L+ R Sbjct: 295 TVGTVSMDMLAVDLTPCPQAGIGTPVELWG----KEIKIDDVAAAAGTVGYELMCALALR 350 Query: 362 IPREY 366 +P Sbjct: 351 VPVVT 355
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0589	SECA	1055	0.0	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 1055 bits (2729), Expect = 0.0 Identities = 391/905 (43%), Positives = 560/905 (61%), Gaps = 73/905 (8%) Query: 1 MANILKTIIENDKG-EIRRLEKMADKVFKYEDQMAALTDDQLKAKTVEFKERYQNGESLD 59 + +L + + +RR+ K+ + + E +M L+D++LK KT EF+ R + GE L+ Sbjct: 2 LIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLE 61 Query: 60 SLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRTGEGKTLTATMPVYLNAL 119 +L+ EAFAVVRE +KRV G+ + VQ++GG+VL+ + EMRTGEGKTLTAT+P YLNAL Sbjct: 62 NLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNAL 121 Query: 120 SGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLAAKSPMEKKEAYECDITYSTNSE 179 +GKGVHVVTVN+YL++RDA L+ +LGL+VGINL K+EAY DITY TN+E Sbjct: 122 TGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNE 181 Query: 180 IGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEARTPLIVSGANAVETSQLYHMAD 239 GFDYLRDNM E VQR L+YALVDEVDSILIDEARTPLI+SG + ++Y + Sbjct: 182 YGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSS-EMYKRVN 240 Query: 240 HYVKSLDKD------------DYIIDVQSKTIGLSDSGIDKAESYF-------KLENLYD 280 + L + + +D +S+ + L++ G+ E + E+LY Sbjct: 241 KIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYS 300 Query: 281 IENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGLHQAIEA 340 N+ L H + ALRA+ + D+DY+V ++ E++IVD+ TGRTM+GRR+SDGLHQA+EA Sbjct: 301 PANIMLMHHVTAALRAHALFTRDVDYIV-KDGEVIIVDEHTGRTMQGRRWSDGLHQAVEA 359 Query: 341 KEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIPIPTNRPVQ 400 KEGV IQ+E +T ASIT+QN FR+Y+KL+GMTGT TE EF IY + + +PTNRP+ Sbjct: 360 KEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMI 419 Query: 401 RIDHSDLLYASIEAKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKLVAAGVPHEVL 460 R D DL+Y + K +A++ED+K R KGQPVLVGT+++E S+ +S +L AG+ H VL Sbjct: 420 RKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVL 479 Query: 461 NAKNHYKEAQIIMNAGQRGAVTIATNMAGRGTDIKLG----------------------- 497 NAK H EA I+ AG AVTIATNMAGRGTDI LG Sbjct: 480 NAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKA 539 Query: 498 ------EGVRELGGLCVIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFG 551 + V E GGL +IGTERHESRRIDNQLRGRSGRQGD G S+FYLS+ED LM+ F Sbjct: 540 DWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFA 599 Query: 552 SERLKGIFERLNMSE-EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREII 610 S+R+ G+ +L M EAIE +T+ + AQ++VE N+D RKQ+L+YDDV +QR I Sbjct: 600 SDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAI 659 Query: 611 YAQRYDVITADRDLAPEIQAMIKRTIERVVDGHARAKQDEK---LEAILNFAKFNLLPED 667 Y+QR +++ D++ I ++ + + +D + + E+ + + K N D Sbjct: 660 YSQRNELLDVS-DVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLK-NDFDLD 717 Query: 668 SITMDDLSGLPD---KTIKEELFQRALQVYDSQVSKLRDEDAVKEFQKVLILRVVDNKWT 724 + L P+ +T++E + ++++VY + + + ++ F+K ++L+ +D+ W Sbjct: 718 LPIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVG-AEMMRHFEKGVMLQTLDSLWK 776 Query: 725 DHIDALDQLRNAVGLRGYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIH---- 780 +H+ A+D LR + LRGYAQ +P EY+ E F MF M+ S++++V + K Q+ Sbjct: 777 EHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEE 836 Query: 781 -----EQERPQAEHHISTTATRNIAAHQA---NIPEDLDLSQIGRNELCPCGSGKKFKNC 832 +Q R +AE + A + ++GRN+ CPCGSGKK+K C Sbjct: 837 VEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQC 896 Query: 833 HGKRQ 837 HG+ Q Sbjct: 897 HGRLQ 901
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0586	TCRTETOQM	38	9e-05	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 37.5 bits (87), Expect = 9e-05 Identities = 30/139 (21%), Positives = 53/139 (38%), Gaps = 25/139 (17%) Query: 1 MALPTIAIVGRPNVGKSTLFNRI-----AGERISIV------------EDVEGVTRDRIY 43 M + I ++ + GK+TL + A + V E G+T Sbjct: 1 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60 Query: 44 ATGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEYV 103 + +W N ++IDT G D A + ++ D + ++S K+G+ + Sbjct: 61 TSFQWENTKVNIIDTPGHMDFLA--------EVYRSLSVLDGAILLISAKDGVQAQTRIL 112 Query: 104 ARKLYKTHKPVILAVNKVD 122 L K P I +NK+D Sbjct: 113 FHALRKMGIPTIFFINKID 131

smi_0575

smi_0539

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0575	-1	28	4.167568	chromosome replication initiation
smi_0574	0	31	4.039224	transcriptional regulator
smi_0573	1	26	4.426997	GntR family transcriptional regulator
smi_0572	1	25	4.364787	ABC transporter, ATP binding domain, ABC-type
smi_0571	1	18	2.424394	ABC transporter permease
smi_0570	-2	14	3.031388	ABC exporter ATP binding/membrane-spanning
smi_0569	0	14	2.654457	LrgB family protein
smi_0568	-1	14	3.559959	effector of murein hydrolase, LrgA family
smi_0567	-2	13	3.313414	transcriptional regulator
smi_0566	0	16	3.094759	hypothetical protein
smi_0565	1	21	4.977967	thioredoxin
smi_0564	0	20	4.224746	7-cyano-7-deazaguanine synthase QueC
smi_0563	-1	18	3.651414	6-pyruvoyl-tetrahydropterin synthase
smi_0562	-1	20	2.828902	7-cyano-7-deazaguanosine (preQ0) biosynthesis
smi_0561	0	18	-0.756838	7-cyano-7-deazaguanine reductase
smi_0560	0	17	-0.722363	aquaporin Z-water channel protein
smi_0558	-1	18	-4.607675	acetoin reductase
smi_0557	-2	17	-4.706852	hypothetical protein
smi_0556	-2	22	-6.632028	oligoendopeptidase F
smi_0555	-1	25	-7.209634	16S rRNA (uracil(1498)-N(3))-methyltransferase
smi_0554	-1	32	-4.182156	ribosomal protein methyltransferase
smi_0553	3	23	-2.730230	7,8-dihydro-8-oxoguanine-triphosphatase
smi_0552	1	21	0.136633	hypothetical protein
smi_0551	0	17	2.417525	chromosome segregation helicase, ATPase, AAA
smi_0550	1	15	3.603304	*transcriptional regulator
smi_0549	0	16	4.041745	NADPH:quinone reductase and related Zn-dependent
smi_0548	0	15	4.217338	hypothetical protein
smi_0547	0	15	4.112622	transcriptional activator, Mga-like regulatory
smi_0546	-1	14	3.759992	hypothetical protein
smi_0545	-1	16	2.817723	hypothetical protein
smi_0544	-2	14	2.730638	hypothetical protein
smi_0543	-2	15	2.781774	general stress protein 24
smi_0542	-2	18	2.878904	hypothetical protein
smi_0541	-2	21	3.284686	acetyltransferase, GNAT family
smi_0540	-2	22	3.296445	type II secretory pathway, prepilin signal
smi_0539	-2	22	3.077725	tryptophan synthase alpha chain

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0567	DHBDHDRGNASE	133	5e-40	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 133 bits (335), Expect = 5e-40 Identities = 81/251 (32%), Positives = 129/251 (51%), Gaps = 6/251 (2%) Query: 3 KVAIVTGAGQGIGFAIAKRLVQDGFKVGVLDYNPETAEKAVAELSAE--NAFAVVADVSK 60 K+A +TGA QGIG A+A+ L G + +DYNPE EK V+ L AE +A A ADV Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 61 QAEVAQAFQKVVDHFGDLNVVVNNAGVAPTTPLDTITEEQFTRTFGINVGGVIWGSQAAQ 120 A + + ++ G ++++VN AGV + ++++E++ TF +N GV S++ Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 121 AQFKALGHGGKIINATSQAGVVGNPNLTVYGGTKFAVRGITQTLARDLADSGITVNAYAP 180 G I+ S V ++ Y +K A T+ L +LA+ I N +P Sbjct: 129 KYMMD-RRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 181 GIVKTPMMYDIAHEVGKNAGKDDEWG-MQTFAKDITLKRLSEPEDVAAAVSFLAGPDSNY 239 G +T M + + +N + G ++TF I LK+L++P D+A AV FL + + Sbjct: 188 GSTETDMQWSLW--ADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245 Query: 240 ITGQTIIVDGG 250 IT + VDGG Sbjct: 246 ITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0560	PF05272	30	0.015	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.4 bits (68), Expect = 0.015 Identities = 12/56 (21%), Positives = 25/56 (44%), Gaps = 7/56 (12%) Query: 42 MILYGPPGIGKTSIASAIAGTTKY--AFRTFNATVDSKKRLQ-----EIAEEAKFS 90 ++L G GIGK+++ + + G + DS +++ E++E F Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSEMTAFR 654
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0555	PF05043	611	0.0	Transcriptional activator
>PF05043#Transcriptional activator Length = 493 Score = 611 bits (1577), Expect = 0.0 Identities = 467/492 (94%), Positives = 482/492 (97%) Query: 1 MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHS 60 MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHS Sbjct: 1 MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHS 60 Query: 61 STNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCDADSICKEFYISSSSLYRI 120 STNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGC A+SICKEFYISSSSLYRI Sbjct: 61 STNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRI 120 Query: 121 ISQINKVIKKQFQFEISLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSVEPLSQL 180 ISQINKVIK+QFQFE+SLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFS EPLSQL Sbjct: 121 ISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQL 180 Query: 181 LELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLNALMQAEGIEG 240 LELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSL+ LMQAEGIEG Sbjct: 181 LELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEG 240 Query: 241 VAQSFESEYNLSLDEEVVCQLFASYFQKMFFIDENLFLKSVKRDSYVGKSYHLLSDFIDQ 300 VAQSFESEYN+SLDEEVVCQLF SYFQKMFFIDE+LF+K VK+DSYV KSYHLLSDFIDQ Sbjct: 241 VAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQ 300 Query: 301 ISVKYQIEIENKDSLIWHLHNTAHLYRQELSTEFILFDQKGNTIRNFQNIFPKFVSDVKK 360 ISVKYQIEIENKD+LIWHLHNTAHLYRQEL TEFILFDQKGNTIRNFQNIFPKFVSDVKK Sbjct: 301 ISVKYQIEIENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDVKK 360 Query: 361 ELSHYLETLELCSSSMMVNHLSYTFITHTKHLVLNLLQNQPKLKVLVMSNFDQYHAKSVA 420 ELSHYLETLE+CSSSMMVNHLSYTFITHTKHLV+NLLQNQPKLKVLVMSNFDQYHAK VA Sbjct: 361 ELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVA 420 Query: 421 ETLSYYCSNNFELEVWSELELSLESLKDSPYDIIISNFIIPPIENKRLIYSNNVNTVALI 480 ETLSYYCSNNFELEVW+ELELS ESL+DSPYDIIISNFIIPPIENKRLIYSNN+NTV+LI Sbjct: 421 ETLSYYCSNNFELEVWTELELSKESLEDSPYDIIISNFIIPPIENKRLIYSNNINTVSLI 480 Query: 481 SLLNAMMFIRLD 492 LLNAMMFIRLD Sbjct: 481 YLLNAMMFIRLD 492
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0548	PREPILNPTASE	74	4e-18	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 74.5 bits (183), Expect = 4e-18 Identities = 62/264 (23%), Positives = 95/264 (35%), Gaps = 62/264 (23%) Query: 6 FFLVGSILASFLGLVIDRFP-------------------------EQSIISPASHCDSCQ 40 FL ++ SFL +VI R P +++ P S C C Sbjct: 19 VFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGVDEPPYNLMVPRSCCPHCN 78 Query: 41 TPLRPLDLIPILSQVLNRFRCRYCKAPYPVWYALFELSLGLIFLLYFWELL----SLSQV 96 P+ L+ IP+LS + R RCR C+AP Y L EL L+ + L +L+ + Sbjct: 79 HPITALENIPLLSWLWLRGRCRGCQAPISARYPLVELLTALLSVAVAMTLAPGWGTLAAL 138 Query: 97 ILITAGLTLGIYDFRHQEYP-------LLVWVVFHLLLMV--------------CSDWNL 135 +L + L D P L ++F+LL W+L Sbjct: 139 LLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMAGYLVLWSL 198 Query: 136 VMVFFLVLGILAHFIDIRMGAGDFLFLASCALVFSATELLILIQFASATGILAFLLQKKK 195 F L+ G MG GDF LA+ L I++ +S G + Sbjct: 199 YWAFKLLTGKEG------MGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMGIGLILL 252 Query: 196 ER------LPFVPFLLLAACVIIF 213 +PF P+L +A + + Sbjct: 253 RNHHQSKPIPFGPYLAIAGWIALL 276
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0541	PERTACTIN	31	0.010	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 31.2 bits (70), Expect = 0.010 Identities = 15/39 (38%), Positives = 23/39 (58%) Query: 41 ILAYNPVFEIKFENGVLYQNGQVIDRDPLDFLYEVTHKS 79 +L NP E++F+NG + +GQ+ D FL VT K+ Sbjct: 79 VLLENPAAELRFQNGSVTSSGQLFDEGVRRFLGTVTVKA 117

smi_0529

smi_0521

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0529	0	18	-3.537611	ABC transporter, ATP-binding domain/permease,
smi_0528	0	20	-4.544978	ABC transporter, ATP-binding domain/permease,
smi_0527	0	22	-4.821200	hypothetical protein
smi_0526	0	21	-4.244369	hypothetical protein
smi_0525	-2	16	-2.245160	hypothetical protein
smi_0524	-3	12	0.586289	transcriptional regulator, ArsR family
smi_0523	-3	14	1.567384	metal-dependent membrane protease, CAAX amino
smi_0522	-3	18	3.178423	exodeoxyribonuclease III
smi_0521	-2	19	3.113154	hypothetical protein

smi_0477

smi_0411

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0477	3	19	0.390115	hypothetical protein
smi_0476	1	18	-0.701398	transcriptional regulators, MutR family
smi_0475	2	22	-0.250987	transcriptional regulatory protein
smi_0474	2	19	0.226769	hypothetical protein
smi_0473	3	19	0.028487	CH60_SMI 60 kDa chaperonin (protein Cpn60)
smi_0472	4	19	0.569932	CH10_SMI 10 kDa chaperonin (protein Cpn10)
smi_0471	4	18	0.255112	single-stranded DNA-binding protein
smi_0470	3	18	0.765016	transposase, ISSmi2
smi_0469	1	16	0.113723	N-acetylmuramoyl-L-alanine amidase LytA
smi_0468	0	15	-0.488728	antiholin
smi_0467	0	16	0.867006	hypothetical protein
smi_0466	-1	16	0.827893	hypothetical protein
smi_0465	-1	16	0.914588	hypothetical protein
smi_0464	0	15	1.005983	hypothetical protein
smi_0463	2	15	1.288824	hypothetical protein
smi_0462	3	15	1.172484	hypothetical protein
smi_0461	1	19	0.340761	structural phage protein
smi_0460	2	20	0.142181	tail fiber protein
smi_0459	2	20	0.548936	hypothetical protein
smi_0458	2	22	0.477730	phage Mu protein gp47
smi_0457	4	19	0.060979	hypothetical protein
smi_0456	4	20	0.866471	hypothetical protein
smi_0455	5	22	-0.508811	hypothetical protein
smi_0454	5	25	-1.084036	LysM
smi_0453	5	27	-2.636271	tail length tape measure protein
smi_0452	5	25	-2.894041	hypothetical protein
smi_0451	1	23	-4.527524	core tail protein
smi_0450	1	21	-3.300151	sheath tail protein
smi_0449	2	20	-2.285935	hypothetical protein
smi_0448	2	18	-1.047858	hypothetical protein
smi_0447	3	18	-0.767661	prophage pi2 protein
smi_0446	3	19	-0.613281	hypothetical protein
smi_0445	3	19	-0.095533	hypothetical protein
smi_0444	3	19	0.408396	hypothetical protein
smi_0443	3	22	1.454217	main capsid protein Gp34-like protein
smi_0442	1	20	-0.248617	methyl-accepting chemotaxis protein scaffolding
smi_0440	0	21	-0.756838	hypothetical protein
smi_0439	3	21	-0.186663	hypothetical protein
smi_0438	2	18	-1.903335	hypothetical protein
smi_0437	2	19	-4.743976	hypothetical protein
smi_0436	1	24	-5.658502	hypothetical protein
smi_0435	2	23	-4.897569	NAD+-asparagine ADP-ribosyltransferase
smi_0433	2	27	-4.654435	serine protein kinase
smi_0432	2	31	-5.773340	terminase large subunit
smi_0431	2	30	-4.290063	small terminase
smi_0430	4	22	-2.263248	*hypothetical protein
smi_0429	4	20	-1.718807	transcriptional regulator, AbrB family
smi_0428	3	19	-1.839748	transcriptional regulator
smi_0427	3	20	-1.448662	hypothetical protein
smi_0426	4	21	-1.148773	hypothetical protein
smi_0425	4	20	-1.264879	DNA-binding protein
smi_0424	2	21	-1.103714	hypothetical protein
smi_0423	4	23	-1.106528	hypothetical protein
smi_0422	4	21	-0.284862	hypothetical protein
smi_0421	2	21	-1.135941	prophage Pi3 protein
smi_0420	1	20	-2.193511	hypothetical protein
smi_0419	0	26	-2.796054	hypothetical protein
smi_0418	0	26	-5.154001	hypothetical protein
smi_0417	0	25	-4.793535	hypothetical protein
smi_0416	0	26	-5.504119	hypothetical protein
smi_0415	0	29	-5.038068	hypothetical protein
smi_0414	0	26	-4.660824	hypothetical protein
smi_0413	0	26	-4.038088	Helicase
smi_0412	3	27	-3.268380	ATPase involved in DNA replication initiation
smi_0411	2	20	-2.082502	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0473	BCTERIALGSPD	26	0.031	Bacterial general secretion pathway protein D signa...
>BCTERIALGSPD#Bacterial general secretion pathway protein D signature. Length = 660 Score = 25.6 bits (56), Expect = 0.031 Identities = 14/58 (24%), Positives = 27/58 (46%), Gaps = 2/58 (3%) Query: 12 FLSLIPVIGLYFSMKDRATKQENRLTVLE-KDIENLNEFKR-SANKRLDNHDEQNKAI 67 L IPVIG F + + N + + I + +E+++ S+ + +D Q+K Sbjct: 562 LLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRDRDEYRQASSGQYTAFNDAQSKQR 619
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0462	cloacin	35	8e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.5 bits (81), Expect = 8e-04 Identities = 28/86 (32%), Positives = 35/86 (40%), Gaps = 10/86 (11%) Query: 665 GSVGEAFNNGYKFGQG-IDKAVGGFFKGAGDSNGAG----NNFLGD-QGTTPYELSPANS 718 G G N G G I+ G G G S+G+G NN G G+ + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW----GG 58 Query: 719 APGQGDGGQGGGGGGHNPTGGKLDEV 744 G G+GG G GG + TGG L V Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAV 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0445	SALSPVBPROT	46	3e-07	Salmonella virulence plasmid 65kDa B protein signature.
>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature. Length = 591 Score = 45.9 bits (108), Expect = 3e-07 Identities = 47/180 (26%), Positives = 81/180 (45%), Gaps = 26/180 (14%) Query: 399 KWLKNLSSYEVDSIHEYTTAMYEDYNHVLR-------EGKQGFLDKITGGSSQKLSDETK 451 KW S ++ ++ Y+ Y N LR + K+ L + +++ +E K Sbjct: 385 KWAIVEESKQIQALRYYSAQGYSVINKYLRGDDYPETQAKETLLSRDYLSTNEPSDEEFK 444 Query: 452 K----WYNDIEKKSEHIISAISTYKAEKTFKTYRLFNQLEDDFL--VNAVGKTLVIDKGF 505 + NDI + +S++ ++ +L D L +G ++IDK F Sbjct: 445 NAMSVYINDIAEG----LSSLPETDHRVVYRGLKLDKPALSDVLKEYTTIG-NIIIDKAF 499 Query: 506 MSTSLDKAVIEEFGGGDVEIQLNILIKKGQSVGAYIGELSNYANEKEFIIKPNTRFKILS 565 MSTS DKA I + LNI ++KG G +G+++++ E E + PNT+ KI S Sbjct: 500 MSTSPDKAWIN-------DTILNIYLEKGHK-GRILGDVAHFKGEAEMLFPPNTKLKIES 551
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0422	SECA	33	0.003	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 33.3 bits (76), Expect = 0.003 Identities = 20/63 (31%), Positives = 31/63 (49%) Query: 210 YERLAKGKQAIVYTHSVEYAERVAKRFIEQGYQSAVVSGKTPQSERESHMQAFREGELTI 269 ER AKG+ +V T S+E +E V+ + G + V++ K +E QA +TI Sbjct: 443 KERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTI 502 Query: 270 MVN 272 N Sbjct: 503 ATN 505
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0418	CHLAMIDIAOMP	28	9e-04	Chlamydia major outer membrane protein signature.
>CHLAMIDIAOMP#Chlamydia major outer membrane protein signature. Length = 393 Score = 28.0 bits (62), Expect = 9e-04 Identities = 9/23 (39%), Positives = 13/23 (56%) Query: 3 LWKCTCADCGREFDWYDNYPPLE 25 LW+C CA G F + + P +E Sbjct: 201 LWECGCATLGASFQYAQSKPKVE 223
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0414	PF05932	27	0.003	Tir chaperone protein (CesT)
>PF05932#Tir chaperone protein (CesT) Length = 127 Score = 26.7 bits (59), Expect = 0.003 Identities = 5/29 (17%), Positives = 10/29 (34%) Query: 7 RLNVEVSGIEELKEACKEVSKKAEELQEA 35 + E + LK + + +EA Sbjct: 97 SIPREKLSVPTLKREMAGLLEWMRGWREA 125

smi_0396

smi_0366

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0396	1	25	-5.016410	phenylalanyl-tRNA synthetase, beta subunit
smi_0395	1	27	-6.136453	hypothetical protein
smi_0394	-1	14	-2.849884	hypothetical protein
smi_0393	0	14	-3.159218	choline-binding protein Cbp5
smi_0392	0	15	-2.508402	CTP synthase (UTP--ammonia ligase)
smi_0391	-1	13	-1.504010	DNA-dependent RNA Polymerase, delta subunit
smi_0390	0	15	1.681199	transposase, ISSmi1
smi_0389	2	26	3.986292	ABC transporter permease
smi_0388	0	18	2.212123	ABC transporter ATP-binding protein
smi_0387	-1	20	3.439591	TetR family transcriptional regulator
smi_0386	-2	18	2.267978	hypothetical protein
smi_0385	-1	19	2.453039	DNA or RNA helicases of superfamily II
smi_0384	2	21	3.874945	cell filamentation protein Fic-related protein
smi_0383	1	18	3.854113	hypothetical protein
smi_0382	4	23	3.575535	molecular chaperone, HSP90 family
smi_0381	5	26	3.332367	type II DNA modification enzyme
smi_0380	4	26	3.519821	valyl-tRNA synthetase
smi_0379	3	25	3.427973	shikimate kinase
smi_0378	-2	23	2.076770	acetyltransferase, GNAT family
smi_0377	-2	19	1.580036	hypothetical protein
smi_0376	-2	18	1.351118	Helicase
smi_0375	0	17	-0.548702	hypothetical protein
smi_0374	1	18	-1.771050	hypothetical protein
smi_0373	3	20	-3.042552	hypothetical protein
smi_0372	6	23	-4.542846	hypothetical protein
smi_0371	6	22	-3.970513	ribosome-binding factor A
smi_0370	7	24	-2.824793	Translation initiation factor
smi_0369	6	25	-2.265484	L7A family ribosomal protein
smi_0368	6	26	-0.958119	nucleic-acid-binding protein implicated in
smi_0367	4	27	0.405506	transcription termination-antitermination
smi_0366	3	26	1.102602	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0396	HTHTETR	44	7e-08	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 44.2 bits (104), Expect = 7e-08 Identities = 15/76 (19%), Positives = 38/76 (50%) Query: 1 MPPKVKFSKEAIIGTALQLVREEGMASLTARALAEQLGATPRVIFGQFANMAELQAEVIS 60 + + +++ I+ AL+L ++G++S + +A+ G T I+ F + ++L +E+ Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 61 AAEMVVVEYIRKALED 76 +E + E + Sbjct: 65 LSESNIGELELEYQAK 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0391	GPOSANCHOR	32	0.010	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 32.0 bits (72), Expect = 0.010 Identities = 15/101 (14%), Positives = 25/101 (24%), Gaps = 5/101 (4%) Query: 391 LINILKPIISKLISERNKIATQISAEDHMEDERRLEIEREERRKEREARRQEEREKRIAE 450 + L +S + K +S + E E+ E Sbjct: 86 HNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALE-----GAMNFSTADS 140 Query: 451 QKAEILEQKNEHLIETNAQLETQNTLQKVMLQEKDPEKQEL 491 K + LE + L A LE + + L Sbjct: 141 AKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTL 181
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0389	RTXTOXIND	32	0.012	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 31.7 bits (72), Expect = 0.012 Identities = 11/73 (15%), Positives = 26/73 (35%), Gaps = 6/73 (8%) Query: 806 YLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQKERDKQADYQ 865 + +L E + EL ++ +L+ + ++ +AK E + + + Sbjct: 248 AIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEI------LSAKEEYQLVTQLFKNEIL 301 Query: 866 AKYDATVARIDEM 878 K T I + Sbjct: 302 DKLRQTTDNIGLL 314
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0379	TCRTETOQM	86	3e-19	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 85.7 bits (212), Expect = 3e-19 Identities = 45/139 (32%), Positives = 63/139 (45%), Gaps = 18/139 (12%) Query: 442 IMGHVDHGKTTLLDTLRNSRVATGEAG------------------GITQHIGAYQIVENG 483 ++ HVD GKTTL ++L + A E G GIT G Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67 Query: 484 KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIVAI 543 K+ +DTPGH F + R SV D IL+++A DGV QT + + +P I I Sbjct: 68 TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFI 127 Query: 544 NKIDKPGANPERVIGELAE 562 NKID+ G + V ++ E Sbjct: 128 NKIDQNGIDLSTVYQDIKE 146

smi_0340

smi_0334

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0340	1	29	3.552810	glutamine synthetase type 1
smi_0339	0	28	3.812151	transcriptional repressor of the glutamine
smi_0338	2	23	3.187106	hypothetical protein
smi_0337	0	24	3.806062	phosphoglycerate kinase
smi_0336	0	19	3.455939	endo-beta-N-acetylglucosaminidase D
smi_0335	1	19	3.317940	Na/Pi cotransporter II-related protein
smi_0334	1	17	3.125380	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0340	BONTOXILYSIN	26	0.015	Bontoxilysin signature.
>BONTOXILYSIN#Bontoxilysin signature. Length = 1196 Score = 26.0 bits (57), Expect = 0.015 Identities = 11/57 (19%), Positives = 23/57 (40%), Gaps = 7/57 (12%) Query: 1 MKRILIAPVRFY---QRFISPAFPPSCRFEPTCSNYMIEAIEKHGF-KGVLMGLARI 53 I +AP R+Y ++ N++ + E+ F + +++ L RI Sbjct: 36 APNIWVAPERYYGEPLDIAEEYKLDGGIYDS---NFLSQDSERENFLQAIIILLKRI 89

smi_0318

smi_0277

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0318	-2	16	3.393856	ABC transporter permease
smi_0317	-3	15	3.376018	ABC transporter substrate-binding protein
smi_0316	-2	16	2.364678	peptide ABC transporter ATP-binding protein
smi_0315	-3	14	2.544488	peptide ABC transporter ATP-binding protein
smi_0314	-3	13	0.609622	peptide ABC transporter permease
smi_0313	-4	13	-1.014021	peptide ABC transporter permease
smi_0312	-1	17	-3.108028	ABC transporter substrate-binding
smi_0311	-1	19	-4.739360	*CBS domain membrane protein
smi_0310	0	24	-5.957843	DNA-entry nuclease (competence-specific
smi_0309	1	25	-6.153048	hypothetical protein
smi_0308	1	17	-3.261889	UDP-N-acetylglucosamine-1-
smi_0307	0	14	-1.845033	hypothetical protein
smi_0306	-2	14	0.485488	phosphopantetheine adenylyltransferase
smi_0305	-1	16	2.147974	methyltransferase
smi_0304	-1	23	3.910465	aspartate--ammonia ligase (asparagine synthetase
smi_0303	-2	23	4.121211	hypothetical protein
smi_0302	-1	24	4.311557	rRNA/tRNA methylase
smi_0301	-2	23	4.490175	oxaA2
smi_0300	-3	22	4.490668	histidine kinase
smi_0299	-3	23	4.949926	response regulator
smi_0298	-3	22	4.458582	ABC transporter permease
smi_0297	-3	20	4.307540	ABC transporter permease
smi_0296	-2	17	3.127220	ABC transporter ATP-binding protein
smi_0295	-2	17	3.330232	pyruvate-formate lyase activating enzyme
smi_0294	-2	16	2.764245	diaminopimelate decarboxylase
smi_0293	-2	16	2.125524	repressor of purine biosynthetic genes
smi_0292	-2	19	2.097889	transposase, ISSmi1
smi_0291	-3	14	1.569169	cmp-binding factor 1
smi_0290	-2	16	3.074476	hypothetical protein
smi_0289	-2	15	2.494006	thiamine pyrophosphokinase
smi_0288	-1	16	2.267728	pentose-5-phosphate-3-epimerase
smi_0287	-2	17	2.859324	hypothetical protein
smi_0286	-2	15	2.730890	16S rRNA
smi_0285	-2	16	3.393272	hypothetical protein
smi_0284	-2	16	3.177984	hypothetical protein
smi_0283	-2	20	2.788441	amino acid permease
smi_0282	-1	16	2.815581	substrate-binding lipoprotein, oligopeptide
smi_0281	-1	13	2.330480	50S ribosomal protein L34
smi_0280	-2	13	2.757672	aspartate aminotransferase
smi_0279	-1	17	2.129341	hypothetical protein
smi_0278	0	25	2.375316	hypothetical protein
smi_0277	0	35	3.184841	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0315	LPSBIOSNTHSS	150	2e-49	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 150 bits (381), Expect = 2e-49 Identities = 55/155 (35%), Positives = 96/155 (61%), Gaps = 1/155 (0%) Query: 5 IGLFTGSFDPMTNGHLDMIERASKLFDKLYVGIFFNPHKQGFLPLENRKRGLEKAVKHLE 64 ++ GSFDP+T GHLD+IER +LFD++YV + NP+KQ ++ R + KA+ HL Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61 Query: 65 NVKVVSSHDELVVDVAKRLGATCLVRGLRNAADLQYEASFDYYNHQLSPNIETIYLHSRS 124 N +V S L V+ A++ A ++RGLR +D + E N L+ ++ET++L + + Sbjct: 62 NAQVDSFEG-LTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTST 120 Query: 125 EHLYISSSGVRELLKFGQDIACYVPESILEEIRNE 159 E+ ++SSS V+E+ +FG ++ +VP + + ++ Sbjct: 121 EYSFLSSSLVKEVARFGGNVEHFVPSHVAAALYDQ 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0310	60KDINNERMP	114	1e-30	60kDa inner membrane protein signature.
>60KDINNERMP#60kDa inner membrane protein signature. Length = 548 Score = 114 bits (287), Expect = 1e-30 Identities = 60/225 (26%), Positives = 108/225 (48%), Gaps = 21/225 (9%) Query: 35 GFIWNTIGAPMAEAIKYFANDKGLGFGVGIIIVTIIVRLIILPLGIYQSWKATLHSEKMN 94 G++W I P+ + +K+ + G +G III+T IVR I+ PL + + KM Sbjct: 331 GWLW-FISQPLFKLLKWIHSFVG-NWGFSIIIITFIVRGIMYPLT-KAQYTSMA---KMR 384 Query: 95 ALKHVLEPHQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGIGCFPVLLQMPFFSAIY 154 L +P ++E +++ Q + A K ++ GG CFP+L+QMP F A+Y Sbjct: 385 ML----QPKIQAMRERLGDDKQ-RISQEMMALYKAEKVNPLGG--CFPLLIQMPIFLALY 437 Query: 155 FAAQHTEGVAQASYLG----IPLGSPSMILVAFAGILYYLQSLLSLHGVEDEMQREQIKK 210 + + + QA + + P IL G+ + +S V D MQ +K Sbjct: 438 YMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQ----QK 493 Query: 211 MIYMSPLMIVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPK 255 ++ P++ VF L+ P+ + LY++V + I+QQ ++ + + Sbjct: 494 IMTFMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKR 538
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0308	HTHFIS	76	3e-18	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 76.0 bits (187), Expect = 3e-18 Identities = 31/120 (25%), Positives = 58/120 (48%), Gaps = 3/120 (2%) Query: 4 KILVVDDDLAILRLIKKVLEYEKYEVVIRNKIEE-IDLCDFIGFDLILLDIMMP-VSGLE 61 ILV DDD AI ++ + L Y+V I + DL++ D++MP + + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 62 ICQMIRE-QITVPICFITAKDMDEDLVAGINAGADDYIMKPFSMQELLARVKMHLRREER 120 + I++ + +P+ ++A++ + GA DY+ KPF + EL+ + L +R Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0305	PF05272	32	0.003	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 31.6 bits (71), Expect = 0.003 Identities = 12/39 (30%), Positives = 16/39 (41%) Query: 27 AKAGRVTAFLGPNGAGKSSTLRILLGLDKATSGLTKIGD 65 K G G GKS+ + L+GLD + IG Sbjct: 593 CKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGT 631
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0297	FLGHOOKAP1	28	0.047	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 27.6 bits (61), Expect = 0.047 Identities = 8/31 (25%), Positives = 14/31 (45%) Query: 11 LAADYANFEREIKRLEATGAEYAHIDIMDGH 41 A A+ +I RL GA + +++D Sbjct: 171 YAKQIASLNDQISRLTGVGAGASPNNLLDQR 201

smi_0268

smi_0263

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0268	-1	18	3.362565	xanthine/uracil permease
smi_0267	0	19	3.502603	hypothetical protein
smi_0266	1	20	3.319739	alcohol dehydrogenase, propanol-preferring,
smi_0265	1	20	3.456677	phosphotransferase system, mannose-specific
smi_0264	1	19	3.709522	phosphotransferase system, mannose-specific,
smi_0263	4	25	3.001735	mannose-specific phosphotransferase system

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0263	TCRTETOQM	620	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 620 bits (1600), Expect = 0.0 Identities = 181/667 (27%), Positives = 296/667 (44%), Gaps = 57/667 (8%) Query: 9 KTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETHEGASQMDWMEQEQERGITITSAAT 68 K NIG++AHVDAGKTT TE +LY +G I ++G +G ++ D E++RGITI + T Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 69 TAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGAVTVLDSQSGVEPQTETVWRQATEYGV 128 + QW N +VNIIDTPGH+DF EV RSL VLDGA+ ++ ++ GV+ QT ++ + G+ Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121 Query: 129 PRIVFANKMDKIGADFLYSVSTLHDRLQANAHPIQLPIGSEDDFRGIIDLIKMKAEIYTN 188 P I F NK+D+ G D + ++L A +IK K E+Y N Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEI------------------VIKQKVELYPN 163 Query: 189 DLGTDILEEDIPAEYLDQAQEYREKLVEAVAETDEELMMKYLEGEEITNEELKAGIRKAT 248 T+ E + + V E +++L+ KY+ G+ + EL+ Sbjct: 164 MCVTNFTESE---------------QWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRF 208 Query: 249 INVEFFPVLCGSAFKNKGVQLMLDAVIDYLPSPLDIPAIKGINPDTDEEETRPASDEEPF 308 N FPV GSA N G+ +++ + + S + Sbjct: 209 HNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH-------------------RGQSEL 249 Query: 309 AALAFKIMTDPFVGRLTFFRVYSGVLQSGSYVLNTSKGKRERIGRILQMHANSRQEIDTV 368 FKI RL + R+YSGVL V + K K +I + +ID Sbjct: 250 CGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKIDKA 308 Query: 369 YSGDIAAAVGLKDTTTGDSLTDEKAKIILESINVPEPVIQLMVEPKSKADQDKMGIALQK 428 YSG+I + L D K E I P P++Q VEP ++ + AL + Sbjct: 309 YSGEIVILQN-EFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLE 367 Query: 429 LAEEDPTFRVETNVETGETVISGMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRAS 488 +++ DP R + T E ++S +G++ ++V ++ ++ VE + P V Y E R Sbjct: 368 ISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYME--RPL 425 Query: 489 TQARGFFKRQSGGKGQFGDVWIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESM 548 +A + + + + +P G G ++E+++ G + + F AV +G+ Sbjct: 426 KKAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGC 485 Query: 549 ANGVLAGYPMVDVKAKLYDGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTI 608 G L G+ + D K G Y+ S+ F++ A + L++ K A +LEP + I Sbjct: 486 EQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKI 544 Query: 609 TVPEENLGDVMGHVTARRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFM 668 P+E L + + N I+ +P + Y + L + GR + Sbjct: 545 YAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCL 604 Query: 669 MVFDHYE 675 Y Sbjct: 605 TELKGYH 611

smi_0236

smi_0231

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0236	3	23	1.483579	histidine kinase
smi_0235	3	22	1.327547	hypothetical protein
smi_0234	3	25	0.567543	ABC transporter ATP-binding protein
smi_0233	5	35	0.620050	hypothetical protein
smi_0232	5	37	1.862735	hypothetical protein
smi_0231	3	30	1.520649	**********transposase, ISSmi1

smi_0195

smi_0180

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0195	2	23	-0.497862	hypothetical protein
smi_0194	1	20	-1.543537	alcohol dehydrogenase
smi_0193	-2	21	-4.150947	N-acetylglucosamine-6-phosphate deacetylase
smi_0192	0	25	-7.157900	transposase, ISSmi1
smi_0191	3	31	-7.685199	hypothetical protein
smi_0190	2	32	-7.647648	tRNA-guanine transglycosylase (guanine insertion
smi_0189	1	30	-7.090474	hypothetical protein
smi_0188	1	30	-7.285077	pyrrolidone-carboxylate peptidase
smi_0187	1	28	-7.343887	hypothetical protein
smi_0186	1	26	-6.784016	hypothetical protein
smi_0185	1	27	-7.563796	multi antimicrobial extrusion (MATE) family
smi_0184	1	28	-7.721850	threonine synthase
smi_0183	2	29	-8.153022	hypothetical protein
smi_0182	3	29	-8.557292	hypothetical protein
smi_0181	2	30	-7.612184	hypothetical protein
smi_0180	-2	24	-5.169730	hypothetical protein

smi_0115

smi_0100

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0115	2	18	-1.012525	ABC transporter ATP-binding protein
smi_0114	2	19	-1.133506	ABC transporter ATP-binding protein
smi_0113	2	24	-3.246360	hypothetical protein
smi_0112	2	33	-6.150295	hypothetical protein
smi_0111	1	26	-4.112246	transcriptional regulator
smi_0110	-1	23	-3.645215	hypothetical protein
smi_0109	0	20	-3.291068	histidyl-tRNA synthetase
smi_0108	-1	19	-3.627783	hypothetical protein
smi_0107.1	-1	23	-0.945162	positive transcriptional regulator of
smi_0107	-2	17	0.302049	hypothetical protein
smi_0106	0	13	-1.139842	hypothetical protein
smi_0105	0	13	-1.435481	dihydroxyacid dehydratase
smi_0104	1	14	-1.607318	transketolase c-terminal section
smi_0103	0	15	-0.921592	transketolase n-terminal section
smi_0102	0	16	-0.267541	PTS system, IIC component
smi_0101	2	18	-2.821181	PTS system, IIB component
smi_0100	3	30	-2.724533	transcriptional regulator, BglG family

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0101	RTXTOXIND	29	0.029	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 28.6 bits (64), Expect = 0.029 Identities = 33/201 (16%), Positives = 66/201 (32%), Gaps = 28/201 (13%) Query: 74 KDIQQLEGTLVEK--GSESYKSLANQVLIELREIHQEADRLKS----------------- 114 K+I+ +E ++V++ E VL++L + EAD LK+ Sbjct: 97 KEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQIL 156 Query: 115 --YIDADVYNRIDKKVRTVRANI---DVQLERLDRESQVDLENAETEELAPELSQTLANI 169 I+ + + N+ +V + Q + + L + A Sbjct: 157 SRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAER 216 Query: 170 AIDHQAILDKIATSAEGDKEELTAIHSLKMEKF---KTILEGYLKIKANPKNYNRAEERL 226 A +++ + +K L SL ++ +LE K + +L Sbjct: 217 LT-VLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQL 275 Query: 227 EQAKAAIEQFDLELDQVLREL 247 EQ ++ I E V + Sbjct: 276 EQIESEILSAKEEYQLVTQLF 296

smi_2088

smi_2077

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_2088	1	25	-7.741181	serine protease
smi_2087	1	28	-6.878174	23S rRNA
smi_2085	1	26	-7.612612	*competence stimulating peptide (CSP) precursor
smi_2084	1	22	-6.596533	histidine kinase
smi_2083	0	21	-5.693325	response regulator
smi_2080	-1	22	-4.451299	**hypothetical protein
smi_2079	-1	21	-4.542491	hypothetical protein
smi_2078	-1	19	-4.425593	hypothetical protein
smi_2077	-1	19	-2.997563	ABC-F family ATPase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2088	V8PROTEASE	62	1e-12	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 61.6 bits (149), Expect = 1e-12 Identities = 31/165 (18%), Positives = 58/165 (35%), Gaps = 34/165 (20%) Query: 117 IVTNNHVINGASKVDIRLS------------DGTKVPGEIVGADTFSDIAVVKISSEKVT 164 ++TN HV++ L +G +I D+A+VK S + Sbjct: 114 LLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQN 173 Query: 165 -------TVAEFGDSSKLTVGETAIAIGSPLG-SEYANTVTQGIVSSLNRNVSLKSEDGQ 216 A ++++ V + G P ++G ++ Sbjct: 174 KHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITY------------- 220 Query: 217 AISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSV 261 + +A+Q D + GNSG P+ N + +VIGI + +V Sbjct: 221 -LKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAV 264
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2087	56KDTSANTIGN	27	0.037	Rickettsia 56kDa type-specific antigen protein sign...
>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein signature. Length = 533 Score = 27.2 bits (60), Expect = 0.037 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%) Query: 14 KYLKDGIAEYSKRISRFAKLEMIELADEKTPDRASESENQ-KILEI 58 K L D I + I FA + I + D P+ AS + Q KI E+ Sbjct: 262 KVLSDKIIQIYSDIKPFADIAGINVPDTGLPNSASIEQIQSKIQEL 307
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2080	HTHTETR	49	1e-09	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 49.2 bits (117), Expect = 1e-09 Identities = 22/104 (21%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 6 KRLKTKRTIENAMVQLLMEQPFDQISTVKLAEKAGISRSSFYTHYKDKYDMIEHYQSKLF 65 + +T++ I + ++L +Q S ++A+ AG++R + Y H+KDK D+ Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67 Query: 66 HTF-EYIFQKHAHHK-------RDAILEVFEYLESEPLLAALLS 101 E + A R+ ++ V E +E L+ Sbjct: 68 SNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLME 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2079	ICENUCLEATIN	34	0.003	Ice nucleation protein signature.
>ICENUCLEATIN#Ice nucleation protein signature. Length = 1258 Score = 34.0 bits (77), Expect = 0.003 Identities = 59/181 (32%), Positives = 74/181 (40%), Gaps = 33/181 (18%) Query: 479 STSLTGLSSGLTEIQGTLTSKLVPASQSITSGVNAY-TAGVDK---VSQGASQLSEKNST 534 ST G S LT G+ ++ S ++T+G + TAG D G+S S S Sbjct: 982 STQTAGYQSTLTAGYGS--TQTAEHSSTLTAGYGSTATAGADSSLIAGYGSSLTSGIRSF 1039 Query: 535 LTGSLDQLVSGSTTLTQKSSKLTAGVGQLVEKTPELVSGIEKLST---GSNQLNQKSQEL 591 LT GST ++ S LTAG G L+SG T GSNQ+ L Sbjct: 1040 LTAGY-----GSTLISGLRSVLTAGYGS------SLISGRRSSLTAGYGSNQIASHRSSL 1088 Query: 592 IAGVDKLQ-----------SGSSQLADKSSQLISGAS--QLESGANKLADGAGKLAEGGT 638 IAG + Q GSSQ A S LISGA Q+ KL GA G Sbjct: 1089 IAGPESTQITGNRSMLIAGKGSSQTAGYRSTLISGADSVQMAGERGKLIAGADSTQTAGD 1148 Query: 639 K 639 + Sbjct: 1149 R 1149
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_2077	PF05272	32	0.009	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 31.6 bits (71), Expect = 0.009 Identities = 11/30 (36%), Positives = 14/30 (46%) Query: 32 LIGANGAGKSTFLKILAGDIEPTTGHISLG 61 L G G GKST + L G + H +G Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIG 630

smi_1988

smi_1978

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1988	2	19	1.788097	hypothetical protein
smi_1987	-1	18	2.273785	hypothetical protein
smi_1986	-1	19	2.996632	hypothetical protein
smi_1985	-1	20	3.664141	hypothetical protein
smi_1984	-1	20	3.560401	transcriptional regulator
smi_1983	-1	15	3.030792	cyanate permease
smi_1982	-2	17	2.279071	glycosyl transferase
smi_1981	-1	19	2.022990	nucleoside-diphosphate sugar isomerase
smi_1980	0	20	-1.078808	phosphatase
smi_1979	1	23	-5.331153	L-serine dehydratase, alpha subunit
smi_1978	2	29	-7.181608	L-serine dehydratase, beta subunit

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1988	IGASERPTASE	42	4e-06	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 42.0 bits (98), Expect = 4e-06 Identities = 29/165 (17%), Positives = 58/165 (35%), Gaps = 14/165 (8%) Query: 7 ESNDFVKTSSKNKPDEQAQDGADKAEETIPDLDTPIEKNTQLEKEVSQAEAELESQQEEK 66 ++ + K + N + ++ + T K T ++ +A+ E E QE Sbjct: 1064 QNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVP 1123 Query: 67 IETPEDS--EAKTETEEKKALDSTEEEPDLSKETEKVTKAEENQEALSQQKTTTKEPLLL 124 T + S + ++ET + +A + E +P +E Q + T + Sbjct: 1124 KVTSQVSPKQEQSETVQPQAEPARENDP--------TVNIKEPQSQTNTTADTEQPAKET 1175 Query: 125 SKSLESPYIPDQAQKSTDRWKEQVLDFWSWLVEALKSPTSKLETS 169 S ++E P + + E + A PT E+S Sbjct: 1176 SSNVEQPVTESTTVNTGNSVVENPEN----TTPATTQPTVNSESS 1216 Score = 36.6 bits (84), Expect = 2e-04 Identities = 26/133 (19%), Positives = 51/133 (38%), Gaps = 2/133 (1%) Query: 9 NDFVKTSSKNKP-DEQAQDGADKAE-ETIPDLDTPIEKNTQLEKEVSQAEAELESQQEEK 66 N V T++ P + QA + + E I +D E E+ ++E Sbjct: 989 NQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQES 1048 Query: 67 IETPEDSEAKTETEEKKALDSTEEEPDLSKETEKVTKAEENQEALSQQKTTTKEPLLLSK 126 ++ + TET + + E + ++ T+ A+ E Q T TKE + K Sbjct: 1049 KTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEK 1108 Query: 127 SLESPYIPDQAQK 139 ++ ++ Q+ Sbjct: 1109 EEKAKVETEKTQE 1121 Score = 33.5 bits (76), Expect = 0.002 Identities = 32/159 (20%), Positives = 57/159 (35%), Gaps = 21/159 (13%) Query: 20 PDEQAQDGADKAEETIPDLDTPIEKNTQ--LEKEVSQAEAELESQQEEKIETPEDSEAKT 77 P E + E +EKN Q E E E++ K T + A++ Sbjct: 1033 PSETTE----TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQS 1088 Query: 78 ETEEKKALDSTEEEPDLSKETEKVTKAEENQEALSQQKTTTKEPLLLSKSLESPYIPDQA 137 +E K+ + +KET V K EE + +++ T + P + S+ + Sbjct: 1089 GSETKET------QTTETKETATVEK-EEKAKVETEK--TQEVPKVTSQVSPKQEQSETV 1139 Query: 138 QKSTDRWKEQVLDFWSWLVEALKSPTSKLETSSTHSYTA 176 Q + +E +K P S+ T++ A Sbjct: 1140 QPQAEPAREND------PTVNIKEPQSQTNTTADTEQPA 1172
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1986	TCRTETB	28	0.025	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 27.9 bits (62), Expect = 0.025 Identities = 19/103 (18%), Positives = 36/103 (34%), Gaps = 4/103 (3%) Query: 76 NRQILHIALLAL---LAAPIGIPLGIAILVSL-FAILVAALTVILAFFAVSILGIIGGFL 131 N +L+++L + P + L F+I A + + L + G + Sbjct: 29 NEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIII 88 Query: 132 FLVESFTVLAQAKSAFILIFGSGLLAIGASSLVLLGISYVARF 174 S +LI + GA++ L + VAR+ Sbjct: 89 NCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARY 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1984	TCRTETA	44	5e-07	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 44.4 bits (105), Expect = 5e-07 Identities = 64/360 (17%), Positives = 118/360 (32%), Gaps = 17/360 (4%) Query: 6 LFFVPGIILIGVSLRTPFTVLPIILGDISQGLGVEVSSLGVLTSLPLLMFTLFSLFSTRL 65 + + +G+ L P VLP +L D+ + G+L +L LM + L Sbjct: 10 ILSTVALDAVGIGLIMP--VLPGLLRDLVHS-NDVTAHYGILLALYALMQFACAPVLGAL 66 Query: 66 AQKIGLEHFFTYSLFFLTIGSLIRLI--NLPLLYLGTL---MVGASIAVINVLLPSLIQA 120 + + G SL + I L +LY+G + + GA+ AV + + Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDG 126 Query: 121 NQ-PKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGLILLLTLLCLATFLVWLP-- 177 ++ + GF++ + M L + A + L FL+ Sbjct: 127 DERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHK 186 Query: 178 -NHRYNHRLAPQTKQKSQTKVMHNKQVWAVIVFAGFQSLLFYTAMTWLPTMAIHAGLSSH 236 R R A + + VF Q + A W+ + Sbjct: 187 GERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDAT 246 Query: 237 EAGLLTSIFSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGMVGISMLFFPVGNFFYW 296 G+ + F ++ I + R LML + +A G +L F W Sbjct: 247 TIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGM--IADGTGYILLAFATR---GW 301 Query: 297 LAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSFDL 356 +A +++ A+ + + + E+ QL G + + GP LF + Sbjct: 302 MAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAA 361
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1983	BCTERIALGSPF	38	5e-05	Bacterial general secretion pathway protein F signa...
>BCTERIALGSPF#Bacterial general secretion pathway protein F signature. Length = 408 Score = 37.5 bits (87), Expect = 5e-05 Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 10/85 (11%) Query: 186 DLLWLNMIATGAKTGNLDQILCQVRVGAGMFERRGGLRYLKLYRQARQRMLKRGQISYME 245 + L+ M+A G +G+LD +L ++ A E+R +R + +Q M+ Sbjct: 132 ERLYCAMVAAGETSGHLDAVLNRL---ADYTEQRQQMR-----SRIQQAMIY--PCVLTV 181 Query: 246 YAKSVAIQMVVALCPGFVRQFIFMK 270 A +V ++ + P V QFI MK Sbjct: 182 VAIAVVSILLSVVVPKVVEQFIHMK 206
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1982	NUCEPIMERASE	81	9e-19	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 81.0 bits (200), Expect = 9e-19 Identities = 50/284 (17%), Positives = 91/284 (32%), Gaps = 57/284 (20%) Query: 294 TILVTGAGGSIGSEICRQ----------VSRFNPERIVLLGHGENSIYLVYHELIRKFQG 343 LVTGA G IG + ++ + N V L EL+ + Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQAR-------LELLAQ--- 51 Query: 344 IDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAR 403 + D+ D + + +F V+ + V NP +N+ G N+ Sbjct: 52 PGFQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILE 111 Query: 404 AVDEAKVPKMVMIST---------------DKAVNPPNVMGATKRVAELIVTGFNQRSQS 448 K+ ++ S+ D +P ++ ATK+ EL+ ++ Sbjct: 112 GCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL 171 Query: 449 TYCAVRFGNVLGSRGS---VIPVFERQIAEGGPVTV-TDFRMTRYFMTI----------- 493 +RF V G G + F + + EG + V +M R F I Sbjct: 172 PATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQ 231 Query: 494 -------PEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKM 530 + + A V+ + PV++ D + + Sbjct: 232 DVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQAL 275
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1978	PF03544	30	0.004	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 30.3 bits (68), Expect = 0.004 Identities = 19/64 (29%), Positives = 24/64 (37%), Gaps = 3/64 (4%) Query: 66 VHMIYVGQELVIDGPAAPVAPASTTYEAPAAQDE--AVSATVAETIEVEEETPAASGTVA 123 ++Y VI+ PA P P S T APA + AV +E E E Sbjct: 30 AGLLYTSVHQVIELPA-PAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPK 88 Query: 124 EETV 127 E V Sbjct: 89 EAPV 92

smi_1540

smi_1527

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1540	2	18	0.361601	ATPase component of ABC transporter with
smi_1539	2	16	0.261605	cation efflux family protein
smi_1538	2	16	0.277201	yybP-ykoY element
smi_1537	1	14	-0.326347	cation-transporting ATPase, E1-E2 family
smi_1536	1	17	0.535620	polypeptide deformylase
smi_1535	1	19	0.694375	hypothetical protein
smi_1534	1	19	0.847732	hypothetical protein
smi_1533	2	23	1.316970	hypothetical protein
smi_1532	2	22	1.684477	cell wall surface anchor family protein
smi_1531	2	23	2.587496	N-acetyl-beta-hexosaminidase
smi_1530	0	14	2.525274	transposase
smi_1529	-1	17	2.955842	transposase, IS1167
smi_1528	-1	18	3.666721	beta-galactosidase
smi_1527	-1	19	3.887869	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1540	FLGMRINGFLIF	30	3e-04	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 29.9 bits (67), Expect = 3e-04 Identities = 9/28 (32%), Positives = 14/28 (50%) Query: 32 KKDKFLSILTSLAGIALVLVAVWLGWPK 59 ++ F+ L + LVLV W+ W K Sbjct: 450 QQQSFIDQLLAAGRWLLVLVVAWILWRK 477
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1538	V8PROTEASE	74	1e-15	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 74.3 bits (182), Expect = 1e-15 Identities = 68/344 (19%), Positives = 127/344 (36%), Gaps = 40/344 (11%) Query: 86 PKTEEELLAKEKETATSSAVSDTLPEELRGKLNKAEENGRTASKEELEKEDK--SLVPED 143 T L++ A SS D P++ + + + + + + LE+ + ++P + Sbjct: 15 TLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNN 74 Query: 144 ----VAKTKNGVLNYGATVEIKSSAG----LGSGIVIGENLVLSVSHNFIKDVPDGNNRK 195 + T NG +Y I+ A + SG+V+G++ +L+ H D + Sbjct: 75 DRHQITDTTNG--HYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPH-AL 131 Query: 196 VADNVESDGDVYTVSYKGAPDVKFSKNDVKHWDREGFLKGYKNDLAIVKL------RTPL 249 A + D Y P+ F+ + + EG DLAIVK + Sbjct: 132 KAFPSAINQDNY-------PNGGFTAEQITKYSGEG-------DLAIVKFSPNEQNKHIG 177 Query: 250 ANAPVEVIDKPSTIKVGDKVHVFGYPKGELDPILNTTVEDINNHGEGVRGISYQGS-EPG 308 + + +V + V GYP + + + I + Y S G Sbjct: 178 EVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKIT--YLKGEAMQYDLSTTGG 235 Query: 309 ASGGGIFDENGKLIGIHQNGVSGKRSGGILFSPAQLEWIQNYIKGIETTKPAGLDALDKQ 368 SG +F+E ++IGIH GV + +G + + ++N++K D Sbjct: 236 NSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINEN----VRNFLKQNIEDIHFANDDQPNN 291 Query: 369 VEDKEEKPKEDKPQEEKPADNKPAENKPADNKPAENKPADNKPA 412 ++ + D P +N N P + +N +DN A Sbjct: 292 PDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDA 335
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1537	GPOSANCHOR	39	4e-04	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 38.5 bits (89), Expect = 4e-04 Identities = 17/74 (22%), Positives = 29/74 (39%), Gaps = 4/74 (5%) Query: 11 HYSIRKFTIGAASVMIGASIFGAGMVQA----AETEGTAETEGTVTQAQPLDKLPADIAA 66 HYS+RK G ASV + ++ GAG+V + ++T+ + DK + Sbjct: 9 HYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADKFEIENNT 68 Query: 67 AIEKAEASAGTVDG 80 K + Sbjct: 69 LKLKNSDLSFNNKA 82
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1534	GPOSANCHOR	50	1e-07	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 49.7 bits (118), Expect = 1e-07 Identities = 39/203 (19%), Positives = 72/203 (35%), Gaps = 5/203 (2%) Query: 11 DKRCHYSIRKFAIGVASVMIGASIFGIS-AVQAEEAASSNTQTEETTVHQAQP-LDKLPD 68 + HYS+RK G ASV + ++ G V E ++ T+++ T+ + Q DK Sbjct: 5 NTNRHYSLRKLKTGTASVAVALTVLGAGLVVNTNEVSAVATRSQTDTLEKVQERADKFEI 64 Query: 69 DVAAAIAKADENGGR-EFVKPKSELAEDKVTKDTETTRPANDGSHELASPKVETPNKVEE 127 + K + + +K ++ ++++ E R + E AS E + + Sbjct: 65 ENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKAD 124 Query: 128 GNKAEDKQKSEEANPKPVESAVTAGTEVRDDAKKTSEKDQVKQTTDIKSSSEKTQALSKE 187 KA + + + A K EK + S K + L E Sbjct: 125 LEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAE 184 Query: 188 SSKADVEKEKQLLSDRKQDFNKD 210 KA +E + L + Sbjct: 185 --KAALEARQAELEKALEGAMNF 205
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1532	PF05043	292	1e-95	Transcriptional activator
>PF05043#Transcriptional activator Length = 493 Score = 292 bits (748), Expect = 1e-95 Identities = 201/488 (41%), Positives = 299/488 (61%), Gaps = 2/488 (0%) Query: 1 MRNLLSTKDQRQLRLMETLIQNRNWLRLHELADKLGCTERILKSDLNELRTAFPTIDIQS 60 MR+LLS K RQL L+E L +++ W ELA+ L CTER +K DL+ +++AFP + S Sbjct: 1 MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHS 60 Query: 61 SINGIMIDLNMQTSVEDVYQHFLAHSQSFQLLEYLFFNEGLPIYRTLENLHSSRANLYRL 120 S NGI I + +E VY HF HS F +LE++FFNEG + + S ++LYR+ Sbjct: 61 STNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRI 120 Query: 121 GRNITKTLSTQFQIELSFTPSEIRGNEIDIRYFYAQYFSERYYFLDWPFPYIPEEDLTEF 180 I K + QFQ E+S TP +I GNE DIRYF+AQYFSE+YYFL+WPF E L++ Sbjct: 121 ISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQL 180 Query: 181 ADFFYKITNYPMHFSIYRMYKLMLAISIYRIKNGHFIDLPNH-FYDEYYPLLMGIPNFEE 239 + YK T++PM+ S +RM KL+L ++YRIK GHF+++ F D+ LM E Sbjct: 181 LELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGIEG 240 Query: 240 TLVYFSEKLGLEITPDIIAQIFISFIQNNLFLDPQEFLNSLEENSEARYSYQLLSQILER 299 F + + + +++ Q+F+S+ Q F+D F+ ++++S SY LLS +++ Sbjct: 241 VAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSDFIDQ 300 Query: 300 LSKQYQITFTNHDELIWHLHNTAFFESQEIFSTPILFEQKTLTIKKFEVYFPDFMASARQ 359 +S +YQI N D LIWHLHNTA QE+F+ ILF+QK TI+ F+ FP F++ ++ Sbjct: 301 ISVKYQIEIENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKFVSDVKK 360 Query: 360 ELAQYRQAIGKHDHPEQLEHLMYTILTHAENLSTLLLENRPPIKVLIISNFDHALSLTFV 419 EL+ Y + + + HL YT +TH ++L LL+N+P +KVL++SNFD + Sbjct: 361 ELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFDQYHAKFVA 420 Query: 420 DMLSYYCNNRFIFDIWDELKTSPEILNQTDYDIIVSNFYIPGI-TKKFICRNHLSIMDLV 478 + LSYYC+N F ++W EL+ S E L + YDII+SNF IP I K+ I N+++ + L+ Sbjct: 421 ETLSYYCSNNFELEVWTELELSKESLEDSPYDIIISNFIIPPIENKRLIYSNNINTVSLI 480 Query: 479 NHLNTLSN 486 LN + Sbjct: 481 YLLNAMMF 488
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1531	TONBPROTEIN	51	2e-08	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 51.1 bits (122), Expect = 2e-08 Identities = 26/81 (32%), Positives = 31/81 (38%), Gaps = 4/81 (4%) Query: 2882 PAQPTPNVPIPEVPVK-PVPAQPTPNVPTPEVPVQPTPVVPTPEVPVKPVPAVPEQP--- 2937 +P V P PV P P P E PV P P+ KPV V EQP Sbjct: 54 DLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRD 113 Query: 2938 VVPTPAQPATPVNANPVVSTT 2958 V P ++PA+P T Sbjct: 114 VKPVESRPASPFENTAPARLT 134 Score = 41.1 bits (96), Expect = 3e-05 Identities = 22/78 (28%), Positives = 26/78 (33%), Gaps = 1/78 (1%) Query: 2884 QPTPNVPIPEVPVKPVPAQPTPNVPTPEVP-VQPTPVVPTPEVPVKPVPAVPEQPVVPTP 2942 P P PI V P +P V P P V+P P P K P V E+P Sbjct: 38 LPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPK 97 Query: 2943 AQPATPVNANPVVSTTVK 2960 +P VK Sbjct: 98 PKPKPVKKVQEQPKRDVK 115 Score = 39.6 bits (92), Expect = 1e-04 Identities = 26/100 (26%), Positives = 31/100 (31%), Gaps = 9/100 (9%) Query: 2868 PEVPEVPRLDIPTVPAQPTPNVPIPEVPV---KPVPAQPTPNVPTPEVPVQPTPVVPTPE 2924 V P + P P E PV KP P P +V QP V Sbjct: 59 QAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVK--- 115 Query: 2925 VPVKPVPAVPEQPVVPTPAQPATPVNA--NPVVSTTVKEN 2962 PV+ PA P + P +T A PV S Sbjct: 116 -PVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPR 154 Score = 33.8 bits (77), Expect = 0.007 Identities = 16/52 (30%), Positives = 19/52 (36%), Gaps = 1/52 (1%) Query: 2893 EVPVKPVPAQPTPNVPTPEVPVQPTPVVPTPEVPVK-PVPAVPEQPVVPTPA 2943 +V P PAQP ++P V P PV P P P P A Sbjct: 34 QVIELPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEA 85 Score = 32.7 bits (74), Expect = 0.016 Identities = 30/135 (22%), Positives = 38/135 (28%), Gaps = 17/135 (12%) Query: 2811 VTPSNDKPVPPTPNMPEGPKFAMPEPPVHELPEFNGGVPGMPEVHELPEFNSGVPGMPEV 2870 VTP++ +P PE PEP P P V E P+ P Sbjct: 50 VTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPP-----KEAPVVIEKPKPKPKPKPKPV- 103 Query: 2871 PEVPRLDIPTVPAQPTPNV-PIPEVPVKPVPAQPTPNVPTPEVPVQPTPVVPTPEVPVKP 2929 V QP +V P+ P P + + P V P Sbjct: 104 --------KKVQEQPKRDVKPVESRPASPFENTAPARLTSS--TATAATSKPVTSVASGP 153 Query: 2930 VPAVPEQPVVPTPAQ 2944 QP P AQ Sbjct: 154 RALSRNQPQYPARAQ 168
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1529	TCRTETA	34	0.001	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 34.0 bits (78), Expect = 0.001 Identities = 48/313 (15%), Positives = 106/313 (33%), Gaps = 14/313 (4%) Query: 43 GLLESIFHTTSLLCEIPSGMLADRYSYKTNLYLSRIAGIVSSILMLAGQGNFWIYALAMA 102 G+L +++ C G L+DR+ + L +S V +M W+ + Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATA-PFLWVLYIGRI 104 Query: 103 VSALSYNFDSGTSAAMVYDSAVEAGLKERYLSISSFLSGVSEGTQSLGTVLAGFFVHGQL 162 V+ ++ +G A + + R+ F+S G VL G Sbjct: 105 VAGITGA--TGAVAGAYIADITDGDERARHF---GFMSACFGFGMVAGPVLGGLMGGFSP 159 Query: 163 HLTYYIMIATSIIVLFLIWMLKEPSVKVEKADSVTMKQIMWTVKDELKRN-----PMLFN 217 H ++ A + + L S K E+ + + + R ++ Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPESHKGER-RPLRREALNPLASFRWARGMTVVAALMAV 218 Query: 218 WMILSQIVGVLMCMFYFYYQNQLPDLSGWQISAVMLLGSLLNIVA-VYLASKIGKNYAAL 276 + I+ + V ++ + +++ I + +L+ +A + + Sbjct: 219 FFIMQLVGQVPAALWVIFGEDRF-HWDATTIGISLAAFGILHSLAQAMITGPVAARLGER 277 Query: 277 RLFPILVLLTGVTYMLSYFGTPLIYILIYLISNALHALFQPIFDNDLQGRLPSEVRATML 336 R + ++ G Y+L F T ++ A + P L ++ E + + Sbjct: 278 RALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQ 337 Query: 337 SVYSMMFSLSMIV 349 + + SL+ IV Sbjct: 338 GSLAALTSLTSIV 350
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1527	FLGPRINGFLGI	29	0.027	Flagellar P-ring protein signature.
>FLGPRINGFLGI#Flagellar P-ring protein signature. Length = 373 Score = 29.1 bits (65), Expect = 0.027 Identities = 8/21 (38%), Positives = 10/21 (47%) Query: 31 DILSLTLGEPDFTTPKNIQDA 51 L L L PDF+T + D Sbjct: 191 VNLVLQLRNPDFSTAVRVADV 211

smi_1503

smi_1496

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1503	-2	13	0.133613	hypothetical protein
smi_1502	-2	12	0.862748	cystathionine gamma-synthase
smi_1501	0	11	0.670862	MalY, bifunctional PLP-dependent enzyme
smi_1500	-2	11	0.539942	abortive infection bacteriophage resistance
smi_1499	-1	11	0.333900	hypothetical protein
smi_1498	2	13	0.155235	HepA, superfamily II DNA/RNA helicases, SNF2
smi_1497	1	18	1.772748	hypothetical protein
smi_1496	-1	18	1.975310	UDP-N-acetylmuramate-alanine ligase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1503	BLACTAMASEA	29	0.016	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 28.6 bits (64), Expect = 0.016 Identities = 15/54 (27%), Positives = 31/54 (57%), Gaps = 1/54 (1%) Query: 3 EERFPLVSDDEIMLTEMPVMDLYDESDFISNIKGEYRDKNYLEWAPITEEKPAK 56 +ERFP++S +++L V+ D D K YR ++ ++++P++E+ A Sbjct: 59 DERFPMMSTFKVVLC-GAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLAD 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1501	SACTRNSFRASE	35	3e-05	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 35.3 bits (81), Expect = 3e-05 Identities = 19/76 (25%), Positives = 33/76 (43%), Gaps = 4/76 (5%) Query: 39 ESIRKCADTFLLARDENKLLGYI-LSSPQSDNPQCLKIHSLVIEADHQRQGLGTLLLAAL 97 + + L EN +G I + S + I + + D++++G+GT LL Sbjct: 58 SYVEEEGKAAFLYYLENNCIGRIKIRSNWNGY---ALIEDIAVAKDYRKKGVGTALLHKA 114 Query: 98 KEVAVELDYKGFRLES 113 E A E + G LE+ Sbjct: 115 IEWAKENHFCGLMLET 130
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1500	PHPHTRNFRASE	28	0.025	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 27.8 bits (62), Expect = 0.025 Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 3/51 (5%) Query: 4 RKARLEDLDRIVEIELENFSAEEAIPRSIFEAHLREIQTSFLVAEKEGRIM 54 + E+L I + + A++A IF AHL + LV +G+I Sbjct: 48 LEKSKEELRAIKDQTEASMGADKA---EIFAAHLLVLDDPELVDGIKGKIE 95
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1499	PF04647	30	0.015	Accessory gene regulator B
>PF04647#Accessory gene regulator B Length = 212 Score = 30.1 bits (68), Expect = 0.015 Identities = 10/46 (21%), Positives = 19/46 (41%) Query: 178 SRRETVKPVKKKKSHLKAFFISLLIFLALISAGGYFGYQYVQSSLL 223 R ++K LK + +++F I A + +Q + LL Sbjct: 127 PRNLISNTEQRKTLKLKTSMVLMVLFGGSIGAYRLYTHQIALAILL 172
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1496	SACTRNSFRASE	33	2e-04	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 32.6 bits (74), Expect = 2e-04 Identities = 16/66 (24%), Positives = 28/66 (42%), Gaps = 2/66 (3%) Query: 52 IAETFGNWLEIEYLFVTEELRGQGTGSKLLQQAESEAKNRNCRFAFVNTYQFQAP--DFY 109 I + + IE + V ++ R +G G+ LL +A AK + + T FY Sbjct: 82 IRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFY 141 Query: 110 KRHGYK 115 +H + Sbjct: 142 AKHHFI 147

smi_1485

smi_1478

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1485	0	11	-0.135444	NAD+ synthetase
smi_1484	0	12	-0.234315	acetyltransferases, including N-acetylases of
smi_1483	0	14	-0.151128	hypothetical protein
smi_1482	0	13	-0.109187	cytochrome c biogenesis protein CcdA
smi_1481	-2	14	2.138330	hypothetical protein
smi_1480	-2	15	2.213263	peptide methionine sulfoxide reductase
smi_1479	-2	15	3.309143	response regulator
smi_1478	-2	16	3.299106	histidine kinase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1485	HTHFIS	95	2e-24	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 94.9 bits (236), Expect = 2e-24 Identities = 35/129 (27%), Positives = 65/129 (50%), Gaps = 6/129 (4%) Query: 4 TILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWDLIQKQVPDIILTDINMPQLN 63 TIL+ +D+ +R L + ++ A YD+ N W I D+++TD+ MP N Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVR---ITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61 Query: 64 GIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKIKQK 123 L +++ P + ++ ++ + F A+ A + G DYL KPF D+ E++G I + Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF---DLTELIGIIGRA 118 Query: 124 LDKEEKEEQ 132 L + ++ Sbjct: 119 LAEPKRRPS 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1484	PF06580	208	2e-64	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 208 bits (530), Expect = 2e-64 Identities = 58/214 (27%), Positives = 104/214 (48%), Gaps = 13/214 (6%) Query: 341 NAMLDQIDQLMTAIRKQEETTRQYELQALSSQINPHFLYNTLDTIIWMAEFQDSQRVVQV 400 N +IDQ K ++ +L AL +QINPHF++N L+ I + +D + ++ Sbjct: 143 NYKQAEIDQW-----KMASMAQEAQLMALKAQINPHFMFNALNNIRALIL-EDPTKAREM 196 Query: 401 TKSLATYFRLAL-NQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEIDEDSTFDNLVLP 459 SL+ R +L + L+DE+ V YL + ++ D+L++E + ++ +P Sbjct: 197 LTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVP 256 Query: 460 KLVLQPLVENALYHGIKEKEGQGHIRVSVQKQDSELVIRIEDDGVGFQDVGDSSQSQLKR 519 +++Q LVEN + HGI + G I + K + + + +E+ G S Sbjct: 257 PMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKES------ 310 Query: 520 GGVGLQNVDQRLKLHFGEHYQMKIDSIPSKGTTV 553 G GLQNV +RL++ +G Q+K+ K + Sbjct: 311 TGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM 344
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1482	IGASERPTASE	39	2e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 39.3 bits (91), Expect = 2e-04 Identities = 56/349 (16%), Positives = 110/349 (31%), Gaps = 24/349 (6%) Query: 198 PSRPDSAEQPQLPVEKQSANEIEK--TAVVQE----RPAVAPETIVENPVVETPVAPAVE 251 + Q +P + EI + A V P+ ET+ EN E+ E Sbjct: 996 NITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNE 1055 Query: 252 ENPVSEKPQTRQ--EESLVEIPFETVTSPDANLAEGQTRIVTAGVKGQRRLVTKVSMVNG 309 ++ Q R+ +E+ + T T+ A + Q + + V Sbjct: 1056 QDATETTAQNREVAKEAKSNVKANTQTNEVAQSGS-------ETKETQTTETKETATVEK 1108 Query: 310 QEVREVVEDQVVQNP--VSQVIAVGTKKE-VQPAPTPTPQAEPTHQVAKGTQEEGKTGQ- 365 +E +V ++ + P SQV + E VQP P + +PT + + + T Sbjct: 1109 EEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADT 1168 Query: 366 ---AITQPTLPEAPVETKGTQEEGKAGQAITQPTLPDAPVEVKGTQEEGKAGQALTQPEL 422 A + E PV T G + + T P + E + + + Sbjct: 1169 EQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPAT-TQPTVNSESSNKPKNRHRRSV 1227 Query: 423 PEAPIEVKGTQEEGKAGQALVQEQLPEYKVTEGTLVETSTTDLDYKTETTEDPTKYTDEE 482 P V+ + L T L + + +++ + Sbjct: 1228 RSVPHNVEPATTSSNDRSTVALCDLTS-TNTNAVLSDARAKAQFVALNVGKAVSQHISQL 1286 Query: 483 TVVRNGEKGSQVTKTTYKTVEGVKTDQVLSTSTEVTKEPVNQQVSRGTK 531 + G+ V+ T+ + S+ + T+ +Q +S + Sbjct: 1287 EMNNEGQYNVWVSNTSMNKNYSSSQYRRFSSKSTQTQLGWDQTISNNVQ 1335
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1478	PF03309	35	3e-04	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 35.1 bits (81), Expect = 3e-04 Identities = 25/126 (19%), Positives = 46/126 (36%), Gaps = 14/126 (11%) Query: 5 IIGIDLGGTSIKFAILTTAGEIQE---KWSIKTNILDEGSHIVDDMIESIQHRLDLLGVA 61 ++ ID+ T +++ +G+ + +W I+T D++ +I L+G Sbjct: 2 LLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTA----DELALTI---DGLIGDD 54 Query: 62 AADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKEKIEKALGIPFFIDNDANVAALG 121 A G S V V W + + + GIP +DN V A Sbjct: 55 AERLTGASGLS--TVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGA-- 110 Query: 122 ERWMGA 127 +R + Sbjct: 111 DRIVNC 116

smi_1362

smi_1355

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_1362	-2	9	1.449979	platelet activating factor
smi_1361	-2	10	1.977891	hypothetical protein
smi_1359	-1	12	2.846260	hypothetical protein
smi_1358	-1	12	2.825177	cI-like repressor, S. pneumoniae bacteriophage
smi_1357	-1	13	2.900305	hypothetical protein
smi_1356	-3	15	2.732511	transposase orfB, ISSmu1
smi_1355	-3	15	1.999537	transposase orfA, ISSmu1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1362	MALTOSEBP	28	0.048	Maltose binding protein signature.
>MALTOSEBP#Maltose binding protein signature. Length = 396 Score = 28.2 bits (62), Expect = 0.048 Identities = 14/37 (37%), Positives = 22/37 (59%) Query: 132 EIGYYSIKDVKLDESGSSATVTFTSKKLHSKGLASST 168 E G Y IKDV +D +G+ A +TF + +K + + T Sbjct: 198 ENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADT 234
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1359	ARGREPRESSOR	36	4e-05	Bacterial arginine repressor signature.
>ARGREPRESSOR#Bacterial arginine repressor signature. Length = 149 Score = 36.0 bits (83), Expect = 4e-05 Identities = 22/98 (22%), Positives = 45/98 (45%), Gaps = 12/98 (12%) Query: 1 MLKTERKQLILEELNQHHVVSLEKLVNLLE-----TSESTVRRDLDELEAENKLRRVHG- 54 M K +R I E + + + + ++LV++L+ +++TV RD+ EL +V Sbjct: 1 MNKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKELHL----VKVPTN 56 Query: 55 GAELPHSLQEEETIQ--EKSVKNLQEKKLLAQKAASLI 90 +SL ++ K ++L + + A+ LI Sbjct: 57 NGSYKYSLPADQRFNPLSKLKRSLMDAFVKIDSASHLI 94
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1358	LCRVANTIGEN	30	0.016	Low calcium response V antigen signature.
>LCRVANTIGEN#Low calcium response V antigen signature. Length = 326 Score = 29.7 bits (66), Expect = 0.016 Identities = 21/78 (26%), Positives = 31/78 (39%) Query: 80 FVQVAEDTRINVKIKADQETEINGTGPTVEPAQLEELKAILSSLTAEDTVVFAGSSAKNL 139 VQ+ +D I++ IK D + V +E LK IL+ ED ++ G L Sbjct: 35 LVQLVKDKNIDISIKYDPRKDSEVFANRVITDDIELLKKILAYFLPEDAILKGGHYDNQL 94 Query: 140 GNVIYKDLIALTRQTGAQ 157 N I + L Q Sbjct: 95 QNGIKRVKEFLESSPNTQ 112
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1356	TYPE3IMSPROT	34	0.002	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 34.0 bits (78), Expect = 0.002 Identities = 13/70 (18%), Positives = 28/70 (40%), Gaps = 1/70 (1%) Query: 37 LIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGILLYLFFFKWIRKQEGLL-SGFFTIFA 95 + + K+ I ++ +I+ + +L L GI I +Q ++ + F + + Sbjct: 140 FLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQILRQLMVICTVGFVVIS 199 Query: 96 GLLLIFEAYL 105 FE Y Sbjct: 200 IADYAFEYYQ 209
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_1355	IGASERPTASE	30	0.003	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.003 Identities = 20/76 (26%), Positives = 30/76 (39%) Query: 65 VKEIQKESPKENTSPTKETNTSQEKAQQEETPKASVKEEKKEEQKASTLDSSTPAPTPSK 124 KE Q KE + KE E + +E PK + + K+EQ + + PA Sbjct: 1092 TKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP 1151 Query: 125 PAADNEKQSNNTPTSE 140 E QS T++ Sbjct: 1152 TVNIKEPQSQTNTTAD 1167

smi_0980

smi_0966

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0980	3	18	0.645930	T-box leader
smi_0979	3	18	0.838604	transposase, ISSmi1
smi_0978	-3	17	2.035185	transposase, ISSmi1
smi_0977	-2	19	2.097453	phosphoenolpyruvate carboxylase
smi_0976	-1	19	2.283967	cell division protein FtsW
smi_0975	0	20	2.282753	peptidylprolyl isomerase
smi_0974	0	20	2.363103	O-methyltransferase
smi_0973	-1	18	2.452202	oligoendopeptidase F
smi_0972	-1	17	2.185182	competence protein CoiA
smi_0971	0	14	0.959929	hypothetical protein
smi_0970	0	13	0.786918	tellurite resistance protein TehB
smi_0969	2	14	1.072247	tmRNA(SsrA)-binding protein
smi_0968	1	15	1.950573	exoribonuclease R
smi_0967	1	16	1.950087	membrane protein involved in protein export
smi_0966	1	12	2.070993	50S ribosomal protein L33

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0980	DPTHRIATOXIN	30	0.019	Diphtheria toxin signature.
>DPTHRIATOXIN#Diphtheria toxin signature. Length = 567 Score = 29.7 bits (66), Expect = 0.019 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 4/49 (8%) Query: 59 NESPEHLTNKEVLYQWLKKETEVQLEHP-LPELKQIAD---VFVNGNLA 103 +ESP ++E Q+L++ + LEHP L ELK + VF N A Sbjct: 263 SESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYA 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0979	FLAGELLIN	34	0.003	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 34.2 bits (78), Expect = 0.003 Identities = 57/378 (15%), Positives = 97/378 (25%), Gaps = 20/378 (5%) Query: 693 DAAKQAAQDAATKANQAIDAATDNA--GVATAQT-----DGIAAIEAVTPTVAVKAAA-- 743 DAA QA + T + + A+ NA G++ AQT + I ++V+A Sbjct: 43 DAAGQAIANRFTSNIKGLTQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGT 102 Query: 744 ---------KAEVAKKLAEKLTALEGTPNATKEEKDAAKQAAQDAATKANQAIDAATDNA 794 + E+ ++L E T + Q + I Sbjct: 103 NSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKI 162 Query: 795 GVATAQTDGIAAIEAVTPTV-AVKAAAKAEVAKKLAEKLTALEGTPNATKEEKDAAKQAA 853 V + DG TV +K++ K + Sbjct: 163 DVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPT 222 Query: 854 QDAATKANQAIDAATDNAGVATAQTDGIAAIEAVTPTVAVKAAAKAEVAKKLAEKLTALE 913 N A T + D ++ T KA A A K + Sbjct: 223 VPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKG 282 Query: 914 GTPNATKEEKDAAKQAAQDAATKANQAIDAATDNAGVATAQTDGIAAIEAVTPTVAVKAA 973 T + + + A AG A + + + V +V Sbjct: 283 VTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQF 342 Query: 974 AKAEVAKKLAEKLTALEGTPNATKEEKDAAKQAAQDAATKA-NQAIDAATDNAGVATAQT 1032 + K + KL+ LE E K A A + T + Sbjct: 343 TFDDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGV 402 Query: 1033 DGIAAIEAVTPTVAVKAA 1050 + +A + Sbjct: 403 STLINEDAAAAKKSTANP 420
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0975	SECGEXPORT	30	3e-04	Protein-export SecG membrane protein signature.
>SECGEXPORT#Protein-export SecG membrane protein signature. Length = 110 Score = 29.9 bits (67), Expect = 3e-04 Identities = 22/78 (28%), Positives = 40/78 (51%), Gaps = 5/78 (6%) Query: 1 MYNLLLTILLVLSVVIVIAIFMQPTK--NQSSNVFDASSGDLFERSKARGFEAVMQRLTG 58 MY LL + L++++ +V I +Q K + ++ +S LF S + F M R+T Sbjct: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNF---MTRMTA 57 Query: 59 ILVFFWLAIALALTVLSS 76 +L + I+L L ++S Sbjct: 58 LLATLFFIISLVLGNINS 75
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0973	TCRTETA	108	2e-28	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 108 bits (272), Expect = 2e-28 Identities = 70/357 (19%), Positives = 144/357 (40%), Gaps = 9/357 (2%) Query: 10 LRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQVAFYAGLAISVSAISAALFSPIWGI 69 L + L I L++P +P + +L V S V + G+ +++ A+ +P+ G Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDL-VHSNDVTAHYGILLALYALMQFACAPVLGA 65 Query: 70 LADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVP 129 L+D++GR+P+++ + + +A P ++ L R++ G+ A A IA Sbjct: 66 LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITD 125 Query: 130 KEKSGSVLGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSLLFLAAVLTILFIKEN 189 ++ G +S G + GP +GG + F F +L L + + E+ Sbjct: 126 GDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLPES 184 Query: 190 FQPVAKEKAIPTKELFTSVKYPYL---LVNLFLTSFVIQFSAQSIGPILALYVRDLGQTE 246 + + S ++ + L F++Q Q + ++ D + Sbjct: 185 HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWD 244 Query: 247 NLLFVSGLIVSSMG-FSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQ 305 G+ +++ G S+ A + G + ++G R L++ Y+L A A+ Sbjct: 245 ATTI--GISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWM 302 Query: 306 LGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRIFAFNQVFFYLGGVVGPMAGSAV 362 L G G +P + A+LS+ + ++ L +VGP+ +A+ Sbjct: 303 AFPIMVLLASG-GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAI 358 Score = 58.7 bits (142), Expect = 1e-11 Identities = 44/178 (24%), Positives = 76/178 (42%), Gaps = 2/178 (1%) Query: 214 LVNLFLTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKL 273 L+ + T + I P+L +RDL + ++ G++++ A V+G L Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66 Query: 274 GDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTP 333 D+ G +L+V+ + + Y + A A L + R + G+ TGA A ++ +T Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITD 125 Query: 334 KAGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLLQFR 391 +R F F F G V GP+ G + G F HA F+A + + L Sbjct: 126 GDERARHFGFMSACFGFGMVAGPVLG-GLMGGFSPHAPFFAAAALNGLNFLTGCFLLP 182
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0970	TCRTETOQM	36	1e-04	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 36.4 bits (84), Expect = 1e-04 Identities = 42/204 (20%), Positives = 79/204 (38%), Gaps = 28/204 (13%) Query: 3 FKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMGIYTTDKEQIVFIDTPG 62 + SG + LG + G + N ++ ++ I T+ + + ++ IDTPG Sbjct: 25 YNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS-------FQWENTKVNIIDTPG 77 Query: 63 IHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGDDMIIERLKAAKVPVILVVNK 122 H DF+ E Y +L +D + ++ A + ++ L+ +P I +NK Sbjct: 78 -HM------DFLAE-VYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINK 129 Query: 123 IDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSRLVDILSENLDEGFQYFPADQIT 182 ID+ + ID D KE + + V ++ N E Q+ D + Sbjct: 130 IDQ-------NGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQW---DTVI 179 Query: 183 DHPERFLVSEMIREKVLHLTREEI 206 + + L M + L E+ Sbjct: 180 EGNDDLLEKYMSGK---SLEALEL 200
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0967	FbpA_PF05833	685	0.0	Fibronectin-binding protein
>FbpA_PF05833#Fibronectin-binding protein Length = 577 Score = 685 bits (1769), Expect = 0.0 Identities = 195/577 (33%), Positives = 321/577 (55%), Gaps = 31/577 (5%) Query: 1 MSFDGFFLHHMVEELRRELVNGRIQKINQPFEQELVLQIRSNRQSHRLLLSAHPVFGRIQ 60 M+ DG FL+ +++EL+ ++NG+I K+NQP + E++L IR R S +LL+S+ + RI Sbjct: 1 MALDGIFLYSIIDELKNTIINGKIDKVNQPEKDEIILNIRKGRLSFKLLISSSSNYPRIH 60 Query: 61 LTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEMTVSNKNEIGDHIQATLII 120 LT T NP + F MVLRKY+ A I I Q+ DRIV + + +E+G + +LII Sbjct: 61 LTDLTKPNPIKAPMFCMVLRKYISNAKIVDIHQINQDRIVVIDFESTDELGFNSIYSLII 120 Query: 121 EIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGSTYIAPPSTESLNPFTIKDEK 180 EIMG+HSN+ L+ K + I++ IKH+ N+YR++ PG Y+ PP + LNPF + Sbjct: 121 EIMGRHSNMTLIRKRDNIIMDSIKHITPDINTYRSIYPGIEYVYPPKSPKLNPFDFSYDM 180 Query: 181 LFEILQ--TQETTAKNLQSLFQGLGRDTANELENILVSDKL---------------STFR 223 + + + + +F G+ + ++E+ L ++ + F+ Sbjct: 181 IENFTKENSLQLNDNIFSKIFTGVSKTLSSEICFRLKNNSIDLSLSNLKEIVEVCKDLFK 240 Query: 224 NFFNQETKPCLTETSFSPVPFA--------NQVGEPFASLSNLLDTYYKDKAERDRVKQQ 275 + + + + S V F + + S S LL+ +Y K + DR+K + Sbjct: 241 EIQSNKFEFNCYTKNNSFVGFYCLNLMSKEDYKKIQYDSSSKLLENFYYAKDKSDRLKSK 300 Query: 276 ASELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILDN 335 +S+L + V N + + K K L ++ + F+ GELLT ++ + + L N Sbjct: 301 SSDLQKIVMNNINRCTKKDKILNNTLKKCEDKDIFKLYGELLTANIYALKKGLSHIELAN 360 Query: 336 YYTNL--PITIALNKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKVTILYLESVETV 393 YY+ + I L++ TP+QN Q Y+K+Y KLK++ + + + + + + YL SV T Sbjct: 361 YYSENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAANEQLLQNEEELNYLYSVLTN 420 Query: 394 LNQA-GLEEIAEIREELIQTGFIRRRQ--REKIQKRKKPEQYLASDGKTIIYVGRNNLQN 450 +N A +EI EI++ELI+TG+I+ ++ + K K KP +++ DG IYVG+NN+QN Sbjct: 421 INNADNYDEIEEIKKELIETGYIKFKKIYKSKKSKTSKPMHFISKDGID-IYVGKNNIQN 479 Query: 451 EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDEVKTDAAELAAYFSQGRLSNLVQVD 510 + LT K A K ++WFH K+IPGSHV++ +D + +AA LAAY+S+ + S+ V VD Sbjct: 480 DYLTLKFANKHDIWFHTKNIPGSHVIVKNIMDIPESTLLEAANLAAYYSKSQNSSNVPVD 539 Query: 511 MIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDPKKISS 547 EVK + KP G KPG V Y+ +T+ VTP + + Sbjct: 540 YTEVKNVKKPNGAKPGMVIYSTNQTIYVTPTNPNLKN 576
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0966	FLGFLGJ	30	0.035	Flagellar protein FlgJ signature.
>FLGFLGJ#Flagellar protein FlgJ signature. Length = 313 Score = 29.7 bits (66), Expect = 0.035 Identities = 24/123 (19%), Positives = 48/123 (39%), Gaps = 27/123 (21%) Query: 469 LLAHSALESDWGRSKIAKDK----NNFFGI----------TAYDTTPYLSA--------- 505 +LA +ALES WG+ +I ++ N FG+ T TT Y + Sbjct: 174 ILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKF 233 Query: 506 KTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGM----NVEYASDPYWGEKIASVMMK 561 + + + + + N T + G + YA+DP++ K+ +++ + Sbjct: 234 RVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQ 293 Query: 562 INE 564 + Sbjct: 294 MKS 296

smi_0852

smi_0845

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0852	-1	11	-1.543063	DNA gyrase, B subunit
smi_0851	-1	13	-1.349179	phosphatase
smi_0850	0	13	-0.212237	transposase, ISSmi1
smi_0849	3	24	-2.713720	4-methyl-5(b-hydroxyethyl)-thiazole
smi_0848	0	23	-3.621852	rod shape determining protein RodA
smi_0847	0	12	-0.066503	ATP-dependent helicase DinG
smi_0846	0	11	1.128540	protease
smi_0845	-1	10	1.163135	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0852	PF06580	36	3e-04	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 36.0 bits (83), Expect = 3e-04 Identities = 13/76 (17%), Positives = 30/76 (39%), Gaps = 9/76 (11%) Query: 314 FRFENRIHRTIVTDQLLLKQL---MTI--LFDNAVKY----TEEDGEIDFLISATDRNLY 364 +FE+R+ + ++ M + L +N +K+ + G+I + + + Sbjct: 234 IQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVT 293 Query: 365 LLVSDNGVGISTEDKK 380 L V + G K+ Sbjct: 294 LEVENTGSLALKNTKE 309
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0851	HTHFIS	86	2e-21	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 85.7 bits (212), Expect = 2e-21 Identities = 34/118 (28%), Positives = 55/118 (46%), Gaps = 1/118 (0%) Query: 2 IKILLVEDDLGLSNSVFDFLDD-FADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGF 60 IL+ +DD + + L DV + +G DL++ D+++P++N F Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63 Query: 61 QVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKR 118 +L +++ PVL+M+A+ + E GA DYL KPF L EL I L Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0849	UREASE	25	0.043	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 25.5 bits (56), Expect = 0.043 Identities = 10/26 (38%), Positives = 13/26 (50%) Query: 13 INPSIGDEIDAWAFGVEPDLLADLVL 38 INP+I + +E ADLVL Sbjct: 411 INPAIAHGLSHEIGSLEVGKRADLVL 436
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0845	DHBDHDRGNASE	92	2e-24	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 91.7 bits (227), Expect = 2e-24 Identities = 63/252 (25%), Positives = 105/252 (41%), Gaps = 24/252 (9%) Query: 3 RRVLITGVSSGIGLAQARLFLEKSYQVYGVDQGENPLL-----EGDFHFLQRDLTLDL-- 55 + ITG + GIG A AR + + VD L D+ Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 56 -EPIFDWCPR-------VDVLCNTAGVLDDYKPLLEQTAQEIQEIFEINYMTPVELTRYY 107 I + R +D+L N AGVL + + +E + F +N +R Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLR-PGLIHSLSDEEWEATFSVNSTGVFNASRSV 127 Query: 108 LTQMLENKKGTIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLAIDYAEAGIQIFGIAP 167 M++ + G+I+ + S + + AY SSK A FTK L ++ AE I+ ++P Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 168 GAVKTAMT--------AADFEPGGLADWVASETPIKRWIEPEEVAEVSLFLASGKVSAMQ 219 G+ +T M A+ G + + P+K+ +P ++A+ LFL SG+ + Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHIT 247 Query: 220 GQILTIDGGWSL 231 L +DGG +L Sbjct: 248 MHNLCVDGGATL 259

smi_0698

smi_0688

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0698	-1	14	-2.631693	hypothetical protein
smi_0697	-2	11	-1.023950	hypothetical protein
smi_0696	-1	10	-0.742847	cell wall-associated serine proteinase
smi_0695	1	14	1.466468	*ABC transporter permease
smi_0694	2	15	1.162095	hypothetical protein
smi_0693	1	12	0.745138	ABC transporter ATP-binding protein
smi_0692	0	16	1.420734	fructose-2,6-bisphosphatase
smi_0691	0	12	1.346673	hypothetical protein
smi_0690	0	13	1.583141	plasmid stabilization system toxin protein,
smi_0688	-2	14	2.477834	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0698	TOXICSSTOXIN	25	0.035	Staphylococcal toxic shock syndrome toxin signature.
>TOXICSSTOXIN#Staphylococcal toxic shock syndrome toxin signature. Length = 234 Score = 25.4 bits (55), Expect = 0.035 Identities = 6/25 (24%), Positives = 13/25 (52%) Query: 45 LLNLDIKVRRLLVKNYSVFYRFDKD 69 + LD ++R L + + ++ DK Sbjct: 166 ISTLDFEIRHQLTQIHGLYRSSDKT 190
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0694	PF06580	31	0.009	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 31.0 bits (70), Expect = 0.009 Identities = 22/102 (21%), Positives = 43/102 (42%), Gaps = 15/102 (14%) Query: 288 ILSLSSV--QELRDDREEIDLLQMTQSLVKDYTLLAKKRELQIDNSLTYQ----QAYLNP 341 + SLS + LR L ++V Y LA + ++ L ++ A ++ Sbjct: 197 LTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQ---FEDRLQFENQINPAIMDV 253 Query: 342 SVMKLILSNLISNAIKHSVL----GGLVRIG--EREGELFIE 377 V +++ L+ N IKH + GG + + + G + +E Sbjct: 254 QVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLE 295
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0693	HTHFIS	86	1e-21	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 85.7 bits (212), Expect = 1e-21 Identities = 29/104 (27%), Positives = 51/104 (49%), Gaps = 1/104 (0%) Query: 2 KILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEKFSSYEVALVLLDIQMPKLNGLE 61 IL+ +D+ IR ++ L+ GY+ ++ ++ + LV+ D+ MP N + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 62 VLAEIRKT-SQVPVLMLTAFQDEEYKMSAFASLADGYLEKPFSL 104 +L I+K +PVL+++A + A A YL KPF L Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDL 108
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0691	PF05272	32	0.002	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 32.0 bits (72), Expect = 0.002 Identities = 18/41 (43%), Positives = 23/41 (56%), Gaps = 4/41 (9%) Query: 28 FEPG-KF-YSII--GESGAGKSTLLSLLAGLDSPVEGSILF 64 EPG KF YS++ G G GKSTL++ L GLD + Sbjct: 589 MEPGCKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDI 629
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0688	ISCHRISMTASE	51	4e-10	Isochorismatase signature.
>ISCHRISMTASE#Isochorismatase signature. Length = 312 Score = 51.2 bits (122), Expect = 4e-10 Identities = 27/88 (30%), Positives = 41/88 (46%), Gaps = 1/88 (1%) Query: 103 KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVASIW 162 K YSAF T+L +R+ +I+TG+ I L TA +A+ V AVA Sbjct: 123 KWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVADFS 182 Query: 163 PENHQFALDHFKNTLGA-KLVDENLNEI 189 E HQ AL++ + D L+++ Sbjct: 183 LEKHQMALEYAAGRCAFTVMTDSLLDQL 210

smi_0637

smi_0631

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0637	0	16	-0.463957	iron-dependent transcriptional regulator
smi_0636	-2	16	1.772436	hypothetical protein
smi_0635	0	16	1.929102	D-tyrosyl-tRNA(Tyr) deacylase
smi_0634	-2	12	1.923938	GTP pyrophosphokinase, stringent response
smi_0633	-1	14	2.378034	hypothetical protein
smi_0632	-2	10	2.145923	metallo-beta-lactamase superfamily protein
smi_0631	-3	14	1.795543	peptidase M13 superfamily

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0637	TCRTETA	38	5e-05	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 38.3 bits (89), Expect = 5e-05 Identities = 53/371 (14%), Positives = 131/371 (35%), Gaps = 13/371 (3%) Query: 2 NRNYKILWLTFLVSNFGDWLRKLALPLLVFEKTGS---PFHMATLYGISFLPWILFSLVG 58 NR ++ T + G L LP L+ + S H L + L + V Sbjct: 4 NRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVL 63 Query: 59 GILADKFKKVYIISICHLISLVILSLLIISFKNDNVLLLLIYILTFLLSSTEPLVHPAFQ 118 G L+D+F + ++ L+SL ++ L +L + +++ Sbjct: 64 GALSDRFGRRPVL----LVSLAGAAVDYAIMATAPFLWVL--YIGRIVAGITGATGAVAG 117 Query: 119 SLLPQIVTDNQLSKANSGIQLIDNTLNLIGPMISGSVLLLINPAKVLWVNALTFLIASVL 178 + + I ++ ++ + + GP++ G ++ +P + A + + Sbjct: 118 AYIADITDGDERARHFGFMSACFGFGMVAGPVL-GGLMGGFSPHAPFFAAAALNGLNFLT 176 Query: 179 ILCIKTPENTLVNISKKESLSKTISIGLHYVKNDKIILSGAILFFGTNFAIHIFQANLVY 238 C PE+ + + + ++ + +FF + A V Sbjct: 177 G-CFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVI 235 Query: 239 YITDVLGYTSFHYGLILSIAGV-GAILGAILAPELIKKFRYGKILSISTMLAGLSTMLLS 297 + D + + G+ L+ G+ ++ A++ + + + L + M+A + +L Sbjct: 236 FGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLG-MIADGTGYILL 294 Query: 298 INTNYIYMGVFLGLSNMFGNINAITYFTLRQKVVKKEMLGRVVSITRMISFASIPLGAYL 357 +M + + G I + + V +E G++ ++ + +G L Sbjct: 295 AFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLL 354 Query: 358 GGILVSHNLTI 368 + + ++T Sbjct: 355 FTAIYAASITT 365
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0634	ADHESNFAMILY	442	e-160	Adhesin family signature.
>ADHESNFAMILY#Adhesin family signature. Length = 309 Score = 442 bits (1138), Expect = e-160 Identities = 296/309 (95%), Positives = 302/309 (97%) Query: 1 MKKLGTLFVLFLSVIALVACASGKKDAASGHKLKVVATNSIIADITKNIAGDKIDLHSIV 60 MKKLGTL VLFLS I LVACASGKKD SG KLKVVATNSIIADITKNIAGDKIDLHSIV Sbjct: 1 MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIADITKNIAGDKIDLHSIV 60 Query: 61 PVGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFSKLVENAKKTENKDYFAVSE 120 P+GQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWF+KLVENAKKTENKDYFAVS+ Sbjct: 61 PIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTKLVENAKKTENKDYFAVSD 120 Query: 121 GVEVIYLEGKNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKL 180 GV+VIYLEG+NEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKL Sbjct: 121 GVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKL 180 Query: 181 DKLDKESKDKFNNIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLV 240 DKLDKESKDKFN IPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLV Sbjct: 181 DKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLV 240 Query: 241 EKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSIAEQGKEGDSYYNMMKYNL 300 EKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSIAEQGKEGDSYY+MMKYNL Sbjct: 241 EKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSIAEQGKEGDSYYSMMKYNL 300 Query: 301 DKIAEGLAK 309 DKIAEGLAK Sbjct: 301 DKIAEGLAK 309
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0632	RTXTOXIND	48	2e-07	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 47.5 bits (113), Expect = 2e-07 Identities = 23/137 (16%), Positives = 53/137 (38%), Gaps = 13/137 (9%) Query: 198 SQFDQKVYNIARLKYQDLAGLNAFSAAYEEKSKQHQEDLE--QALSDNGK-ARLQLLKKE 254 +Q QK N+ + + + + A YE S+ + L+ +L A+ +L++E Sbjct: 200 NQKYQKELNLDKKR-AERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQE 258 Query: 255 GQESLDKGQETLDKSETNLQEGKRRLAAAQARIQVQESQLDLLPQAQREQASAQLTQAKQ 314 + ++ L+ K +L ++ I + + L+ Q + + +L Q Sbjct: 259 NK---------YVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTD 309 Query: 315 ELSKEEDRLKQAEQNLA 331 + L + E+ Sbjct: 310 NIGLLTLELAKNEERQQ 326 Score = 36.0 bits (83), Expect = 7e-04 Identities = 22/129 (17%), Positives = 44/129 (34%), Gaps = 2/129 (1%) Query: 231 QHQEDLEQALSDNGKARLQLLKKEG-QESLDKGQETLDKSETNLQEGKRRLAAAQARIQV 289 + D + S +ARL+ + + S++ + K + Sbjct: 131 GAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSL 190 Query: 290 QESQLDLLPQAQREQASAQLTQAKQELSKEEDRLKQAEQNLAQEKEKLEKHQRVLDDLAE 349 + Q Q Q+ Q L + + E R+ + E EK +L+ +L A Sbjct: 191 IKEQFSTW-QNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAI 249 Query: 350 PKYQVYNRQ 358 K+ V ++ Sbjct: 250 AKHAVLEQE 258
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0631	PF05272	30	0.006	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.4 bits (68), Expect = 0.006 Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 1/27 (3%) Query: 32 KGELVIIL-GASGAGKSTVLNLLGGMD 57 K + ++L G G GKST++N L G+D Sbjct: 594 KFDYSVVLEGTGGIGKSTLINTLVGLD 620

smi_0263

smi_0256

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0263	4	25	3.001735	mannose-specific phosphotransferase system
smi_0262	-1	18	2.692360	hypothetical protein
smi_0261	-2	20	2.388817	hypothetical protein
smi_0260	-1	24	2.292489	transposase, ISSmi5
smi_0259	-1	18	0.855891	aminopeptidase C
smi_0258	0	23	1.886437	16S rRNA pseudouridine(516) synthase
smi_0257	0	25	1.463561	aminopeptidase
smi_0256	-1	19	0.388995	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0263	TCRTETOQM	620	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 620 bits (1600), Expect = 0.0 Identities = 181/667 (27%), Positives = 296/667 (44%), Gaps = 57/667 (8%) Query: 9 KTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETHEGASQMDWMEQEQERGITITSAAT 68 K NIG++AHVDAGKTT TE +LY +G I ++G +G ++ D E++RGITI + T Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 69 TAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGAVTVLDSQSGVEPQTETVWRQATEYGV 128 + QW N +VNIIDTPGH+DF EV RSL VLDGA+ ++ ++ GV+ QT ++ + G+ Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121 Query: 129 PRIVFANKMDKIGADFLYSVSTLHDRLQANAHPIQLPIGSEDDFRGIIDLIKMKAEIYTN 188 P I F NK+D+ G D + ++L A +IK K E+Y N Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEI------------------VIKQKVELYPN 163 Query: 189 DLGTDILEEDIPAEYLDQAQEYREKLVEAVAETDEELMMKYLEGEEITNEELKAGIRKAT 248 T+ E + + V E +++L+ KY+ G+ + EL+ Sbjct: 164 MCVTNFTESE---------------QWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRF 208 Query: 249 INVEFFPVLCGSAFKNKGVQLMLDAVIDYLPSPLDIPAIKGINPDTDEEETRPASDEEPF 308 N FPV GSA N G+ +++ + + S + Sbjct: 209 HNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH-------------------RGQSEL 249 Query: 309 AALAFKIMTDPFVGRLTFFRVYSGVLQSGSYVLNTSKGKRERIGRILQMHANSRQEIDTV 368 FKI RL + R+YSGVL V + K K +I + +ID Sbjct: 250 CGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKIDKA 308 Query: 369 YSGDIAAAVGLKDTTTGDSLTDEKAKIILESINVPEPVIQLMVEPKSKADQDKMGIALQK 428 YSG+I + L D K E I P P++Q VEP ++ + AL + Sbjct: 309 YSGEIVILQN-EFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLE 367 Query: 429 LAEEDPTFRVETNVETGETVISGMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRAS 488 +++ DP R + T E ++S +G++ ++V ++ ++ VE + P V Y E R Sbjct: 368 ISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYME--RPL 425 Query: 489 TQARGFFKRQSGGKGQFGDVWIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESM 548 +A + + + + +P G G ++E+++ G + + F AV +G+ Sbjct: 426 KKAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGC 485 Query: 549 ANGVLAGYPMVDVKAKLYDGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTI 608 G L G+ + D K G Y+ S+ F++ A + L++ K A +LEP + I Sbjct: 486 EQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKI 544 Query: 609 TVPEENLGDVMGHVTARRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFM 668 P+E L + + N I+ +P + Y + L + GR + Sbjct: 545 YAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCL 604 Query: 669 MVFDHYE 675 Y Sbjct: 605 TELKGYH 611
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0259	HTHFIS	73	2e-17	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 72.9 bits (179), Expect = 2e-17 Identities = 24/122 (19%), Positives = 51/122 (41%), Gaps = 2/122 (1%) Query: 2 KLLVAEDQSMLRDAMCQLLTLQPDVESVFQAKNGQEAIQLLEKESVNIAILDVEMPVKTG 61 +LVA+D + +R + Q L+ V N + + ++ + DV MP + Sbjct: 5 TILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62 Query: 62 LEVLEWIRAENLETKVVVVTTFKRPGYFERAVKAGVDAYVLKERNIADLMQTLHTVLEGR 121 ++L I+ + V+V++ +A + G Y+ K ++ +L+ + L Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122 Query: 122 KE 123 K Sbjct: 123 KR 124
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0258	PF06580	40	9e-06	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 40.2 bits (94), Expect = 9e-06 Identities = 66/376 (17%), Positives = 119/376 (31%), Gaps = 67/376 (17%) Query: 1 MLERLKSIHYMFWASLIFMLFPILPVVTGWLSAWHLLIDILFVVAYLGVLTTKSQHLSWL 60 L L M+F I + G + AY + + WL Sbjct: 24 TLTGFGFASLYGSPKLHSMIFNIAISLMGLV----------LTHAYRSFI----KRQGWL 69 Query: 61 YWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKSLHVWTFLLAQVLVVGQLL 120 + + A V + +WF S F + + L VV Sbjct: 70 KLNMGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPLALSIIFNVVVVTF 129 Query: 121 IFQRIEVESLFYLLVILAFVDLMTFGMVRIRIVEDLKEAQAKQNAQINLLLAENERSRIG 180 ++ + F+ + ++ K A Q AQ+ L +++I Sbjct: 130 MWSLLYFGWHFFK-------------NYKQAEIDQWKMASMAQEAQLMAL-----KAQIN 171 Query: 181 QDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELKEIHQISKDSMNEVRTIVENLKS 240 + + + +E + + L + ++ + S+ + Sbjct: 172 PHF---MFNALNNIRALI--------LEDPTKAREMLTSLSELMRYSLRYSNA-----RQ 215 Query: 241 RTLASELETVKKMLEIAGI----EVQVENQLDKASLTQDVESTAAMVLLELATNIIKHAR 296 +LA EL V L++A I +Q ENQ++ A + V M++ L N IKH Sbjct: 216 VSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPP---MLVQTLVENGIKHGI 272 Query: 297 AEKA-----YLKLERTDQELVLTVRDDGKGFA----TVKGNELHTVRDRAATFSG---QV 344 A+ LK + + + L V + G G L VR+R G Q+ Sbjct: 273 AQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQNVRERLQMLYGTEAQI 332 Query: 345 ELVSLKDPTEVRVHLP 360 +L + V +P Sbjct: 333 KLSEKQGKVNAMVLIP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0256	PF05272	29	0.025	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 28.9 bits (64), Expect = 0.025 Identities = 11/32 (34%), Positives = 16/32 (50%) Query: 31 CVALIGPNGAGKTTLLDCLLGDKRITSGQVSI 62 V L G G GK+TL++ L+G + I Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDI 629

smi_0073

smi_0064

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0073	0	22	5.963823	PTS system, IID component
smi_0072	1	21	5.343425	PTS system, IIC component
smi_0071	0	19	4.075012	phosphotransferase system sugar-specific EIIB
smi_0070	-1	19	4.300556	beta-galactosidase 3
smi_0069	-2	21	4.028032	hypothetical protein
smi_0068	-3	20	3.438264	transposase, IS1167
smi_0067	-3	19	2.679636	adenylosuccinate lyase
smi_0066	-1	25	0.940058	5-(carboxyamino)imidazole ribonucleotide
smi_0065	0	26	1.271134	5-(carboxyamino)imidazole ribonucleotide mutase
smi_0064	2	27	0.033474	phosphoribosylglycinamide synthetase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0073	ARGDEIMINASE	30	0.023	Bacterial arginine deiminase signature.
>ARGDEIMINASE#Bacterial arginine deiminase signature. Length = 409 Score = 29.8 bits (67), Expect = 0.023 Identities = 16/94 (17%), Positives = 35/94 (37%), Gaps = 14/94 (14%) Query: 146 DGLALGKGVVVAETVEQAVEAAHEMLLDNKFGDSGA--RVVIEEF--------LDGEEFS 195 D L L KG++V E+ + E L + F + + ++ + LD + Sbjct: 220 DELVLNKGLLVIGISERTEAKSVEKLAISLFKNKTSFDTILAFQIPKNRSYMHLD----T 275 Query: 196 LFAFVNGDKFYIMPTAQDHKRAYDGNKGPNTGGM 229 +F ++ F + + Y P++ + Sbjct: 276 VFTQIDYSVFTSFTSDDMYFSIYVLTYNPSSSKI 309
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0069	BINARYTOXINA	30	0.012	Clostridial binary toxin A signature.
>BINARYTOXINA#Clostridial binary toxin A signature. Length = 454 Score = 30.4 bits (68), Expect = 0.012 Identities = 11/33 (33%), Positives = 15/33 (45%) Query: 193 YSLVRRVFADYTGEEVLPELEGKQLKEVLLEPT 225 YS R+ F DY E E E K L+ + + Sbjct: 93 YSQTRQYFYDYQIESNPREKEYKNLRNAISKNK 125
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0067	FLGMRINGFLIF	32	0.014	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 32.2 bits (73), Expect = 0.014 Identities = 29/127 (22%), Positives = 41/127 (32%), Gaps = 35/127 (27%) Query: 1100 ASSTSPTLFYNDANQHVAKMVETRITNTNSPWLAGVQVGDIHAIPVSHGEGKFV--VTAE 1157 S + NDA A VE+RI L+ + G G VTA+ Sbjct: 220 TQSNTSGRDLNDAQLKFANDVESRIQRRIEAILSPIV-----------GNGNVHAQVTAQ 268 Query: 1158 EFAELRDNGQIFSQYVDFDGKPSMDSKYNPNGSVHAIEGITSKNGQIIGKMGHSERYEDG 1217 +DF K + Y+PNG SK ++ SE+ G Sbjct: 269 ---------------LDFANKEQTEEHYSPNGDA-------SKATLRSRQLNISEQVGAG 306 Query: 1218 LFQNIPG 1224 +PG Sbjct: 307 YPGGVPG 313
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0066	RTXTOXINA	30	0.011	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 30.3 bits (68), Expect = 0.011 Identities = 19/81 (23%), Positives = 32/81 (39%), Gaps = 4/81 (4%) Query: 172 SIVEFYYKNDDLDDPFINDEHVKFLQIADDQQISYLKEETRRINE----LLKAWFAEIGL 227 I + K D L I+ V F + +D + + I + WF + Sbjct: 861 IIDDDGGKEDKLSLADIDFRDVAFKREGNDLIMYKGEGNVLSIGHKNGITFRNWFEKESG 920 Query: 228 KLIDFKLEFGFDKDGKIILAD 248 + + ++E FDK G+II D Sbjct: 921 DISNHEIEQIFDKSGRIITPD 941
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0065	RTXTOXIND	67	3e-14	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 67.2 bits (164), Expect = 3e-14 Identities = 78/465 (16%), Positives = 150/465 (32%), Gaps = 54/465 (11%) Query: 4 EFLESA-EFYNRRYHNFSSRVIVPMSLLLVFLLGFATFAEKEMSLSTRATVEPSRILANI 62 EFL + E V + LV + + E+ + + S I Sbjct: 40 EFLPAHLELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEI 99 Query: 63 QSTSN---NRILVNHLEENKLVKKGELLLQYQEGAEGVQAEAYASQLDMLKDQKKQLEYL 119 + N I+V +E + V+KG++LL+ A G +A+ +Q +L+ + +Q Y Sbjct: 100 KPIENSIVKEIIV---KEGESVRKGDVLLKLT--ALGAEADTLKTQSSLLQARLEQTRYQ 154 Query: 120 QKSLQEGTDYFPEEDKFGYQATFRDYISQAGSLRASTSQQNETIASQNAAASQTQAEIGN 179 S + PE D + I Q + + + Sbjct: 155 ILSRSIELNKLPE-------LKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKEL 207 Query: 180 LISQAEAKIRDYQTAKSAIETGASLGNQNLAYSLYQSYKSQGEENPQAKAQAVAQVEAQL 239 + + A+ + E + + L + S + AV + E + Sbjct: 208 NLDKKRAERLTVLARINRYENLSRVEKSRL--DDFSSLLHKQ----AIAKHAVLEQENKY 261 Query: 240 SQLESSLATYRVQYAGSGSQQAYASGLSSQL-ESLKSQHLAKVGQELTLLDQKILEVESG 298 + + L Y+ Q S+ A + + K++ L K+ Q + LE+ Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKN 321 Query: 299 KKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEGALLAQLYPS---LEKEGKAKLTAY 355 ++ Q + I A + ++ +V L + P LE Sbjct: 322 EERQQASV----IRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTAL------ 371 Query: 356 LSSKDVARIKVGDSVR-------YTTTHDAKNQLFLDSTITSIDATATKTEKGNFF---- 404 + +KD+ I VG + YT L + +I+ A + ++ Sbjct: 372 VQNKDIGFINVGQNAIIKVEAFPYTRYGY------LVGKVKNINLDAIEDQRLGLVFNVI 425 Query: 405 -KIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNK 448 IE T + L G+ ++ TG +S + Y L Sbjct: 426 ISIEENCLSTGNKNIPLSSGMAVTAEIKTGMRSVISYLLSPLEES 470
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0064	ANTHRAXTOXNA	30	0.042	Anthrax toxin LF subunit signature.
>ANTHRAXTOXNA#Anthrax toxin LF subunit signature. Length = 800 Score = 30.1 bits (67), Expect = 0.042 Identities = 34/209 (16%), Positives = 76/209 (36%), Gaps = 26/209 (12%) Query: 306 NLFFMTLLALPIYTVIIFAFMKPFEKMNRDTMEANAVLSSSIIEDINGIETIKSLTSESQ 365 N F ++ ++V++FA + +E NA+ DI + +E + Sbjct: 4 NKFIPNKFSIISFSVLLFAIS------SSQAIEVNAMNEHYTESDIKRNHKTEKNKTEKE 57 Query: 366 RYQKIDKEFVDYLKKSFTYSRAESQQKALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLI 425 +++ V + T + + Q LKK+ +L + G + D + Sbjct: 58 KFKDSINNLVKTEFTNETLDKIQQTQDLLKKIPKDVLEIYSELGGEIYFTDID------L 111 Query: 426 TYNTLLVYFTNPLENIINLQTKLQTAQVANNRLNEVYLVASEFEEKKTV---EDLSLMKG 482 + L + +N +N + + ++ + E K + +D ++ Sbjct: 112 VEHKELQDLSEEEKNSMNSRGE-------KVPFASRFVFEKKRETPKLIINIKDYAINSE 164 Query: 483 EMTFKQVSYKYGYG--RDVLSDINLTIPQ 509 + K+V Y+ G G D++S P+ Sbjct: 165 QS--KEVYYEIGKGISLDIISKDKSLDPE 191

smi_0038

smi_0031

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
smi_0038	3	19	0.488298	hypothetical protein
smi_0037	0	12	0.768431	transposase, ISSpnII
smi_0036	-2	16	2.170512	transposase, ISSpnII
smi_0034	-2	15	2.033314	hypothetical protein
smi_0033	-2	19	2.913574	hypothetical protein
smi_0032	-2	20	2.455321	transposase, ISSmi1
smi_0031	-2	20	1.983152	transposase, ISSmi4

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0038	PF01540	39	4e-05	Adhesin lipoprotein
>PF01540#Adhesin lipoprotein Length = 475 Score = 38.9 bits (90), Expect = 4e-05 Identities = 71/271 (26%), Positives = 110/271 (40%), Gaps = 45/271 (16%) Query: 44 NYDKALEEAVKALEAAKTNSPEEAKAKAD---TIAALKAASDATRKEIVEGFKKGLTVEA 100 +Y K LE K + A T S +EA + D I+ L AA + + E Sbjct: 60 DYSKILETLNKEI-AEATKSFKEAGSYGDYPAIISKLSAAVENAKSE------------- 105 Query: 101 AEKLINQANEKAAEEDKAAAAKEKAAQEEFLANYDQALKDA----IAELEKADTKNDPEL 156 ++ ++QAN+K A+E+ KE A + L+ Q+ D I +LE + D Sbjct: 106 -QQKVDQANKKIADEN--LKIKEGAKELLKLSEKIQSFADTIALTITKLEGKKFQIDETF 162 Query: 157 EKQKKETVAALKAASVQTRS-EIVEGFKQGLTAKQAEGLID------EANKKAAEEDKEA 209 +KQ T+ L S + ++ V K+ + E + E EE K+A Sbjct: 163 KKQLISTIELLNKKSAEVKTFATVNTIKKDFLLSELESFKEFNTSWLEKIVSEWEEVKKA 222 Query: 210 AAKE----EAAQEEFLANYDAALKAAIAELEKAETNSPEEAKAKADTIAALKAASDKTRA 265 +KE +A ++ LA + +K EL K A A TI L + + Sbjct: 223 WSKELAEIKAEDDKKLAEENQKIKEGAKELLKLSEKIQSFADTIALTITKL-----ERKF 277 Query: 266 EIVEGFKQGLTAKQAEKFIDELNKKAAEEDK 296 +I E FK KQ I+ LNKK+ E Sbjct: 278 QIDEKFK-----KQLISTIELLNKKSVEVKT 303 Score = 30.9 bits (69), Expect = 0.013 Identities = 51/180 (28%), Positives = 73/180 (40%), Gaps = 35/180 (19%) Query: 123 EKAAQEEFLANYDQALK--DAIAELEKADTKNDPELEKQKKETVAALK----AASVQTRS 176 +K A++ D ALK +A+AE K + LE KE A K A S Sbjct: 30 DKLAEKNGKEKADAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYGDYP 89 Query: 177 EIVEGFKQGL-TAKQAEGLIDEANKKAAEEDKEAAAKEEAAQEEFLANYDAALKAAIAEL 235 I+ + AK + +D+ANKK A+E+ +K EL Sbjct: 90 AIISKLSAAVENAKSEQQKVDQANKKIADEN-------------------LKIKEGAKEL 130 Query: 236 EKAETNSPEEAKAKADTIAALKAASDKTRAEIVEGFKQGLTAKQAEKFIDELNKKAAEED 295 K E+ ++ ADTIA + + +I E FK KQ I+ LNKK+AE Sbjct: 131 LKLS----EKIQSFADTIALTITKLEGKKFQIDETFK-----KQLISTIELLNKKSAEVK 181
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0037	CHANLCOLICIN	29	0.038	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 29.3 bits (65), Expect = 0.038 Identities = 46/256 (17%), Positives = 91/256 (35%), Gaps = 34/256 (13%) Query: 41 KAKADAQAVAQHIYDVQDQQL--------SILTPAYNLYKEKDAAYNTL-----SARVKE 87 KAKA+ A+ Q + D+ ++ L S A+ A L + ++ Sbjct: 82 KAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARK 141 Query: 88 AYSNLEKLQQEKIRTADLIGDGTALVRKADELKSARTEAAQREAKFTDDVESYENEVNTA 147 EK QE + I A +LK A E +R A +++ ++ E Sbjct: 142 EAEAAEKAFQEAEQRRKEIEREKA--ETERQLKLAEAE-EKRLAALSEEAKAVEIAQKKL 198 Query: 148 KQARVDAVKVTAAAEAAFQAALTSGDDTAIAQTRSNLALAKGRQNAAVKAEKEANEKLAK 207 A+ + VK+ + + L+S R A +K +LA+ Sbjct: 199 SAAQSEVVKMDGEIKTL-NSRLSS--------------SIHARD-AEMKTLAGKRNELAQ 242 Query: 208 AEAELQKAVFDQARIEAALQYMDALETGNDYKITQAAYNLDKKIENAKETISTLESEVEV 267 A A+ ++ D+ + + + D L+ ++ T+ K E ++ ++ E+ + Sbjct: 243 ASAKYKE--LDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINR 300 Query: 268 ARTDRDNALLALENAE 283 D A+ Sbjct: 301 INADITQIQKAISQVS 316
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0036	FLGFLIH	31	4e-04	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 30.9 bits (69), Expect = 4e-04 Identities = 15/35 (42%), Positives = 25/35 (71%) Query: 22 QAEEKGLERGLERGRAEGIEKGREEGIEEGLKVGL 56 QA E+G + G+ GR +G ++G +EG+ +GL+ GL Sbjct: 50 QAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGL 84 Score = 28.6 bits (63), Expect = 0.002 Identities = 15/44 (34%), Positives = 26/44 (59%) Query: 9 EEQALLAQDYALEQAEEKGLERGLERGRAEGIEKGREEGIEEGL 52 E+Q Q A EQ + G+ G ++G +G ++G +G+E+GL Sbjct: 41 EQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGL 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0034	FLGFLIH	40	5e-06	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 40.2 bits (93), Expect = 5e-06 Identities = 28/83 (33%), Positives = 45/83 (54%), Gaps = 8/83 (9%) Query: 215 LDYKSWSEED----RKMFSQLRMREEQALLAQDYALETARAE----GIEQGLERGLERGR 266 L +K+W+ +D + F + EE + + +LE A+ EQG + G+ GR Sbjct: 5 LPWKTWTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQAHEQGYQAGIAEGR 64 Query: 267 AEGIEKGREEGLEQGLERGKAEG 289 +G ++G +EGL QGLE+G AE Sbjct: 65 QQGHKQGYQEGLAQGLEQGLAEA 87 Score = 34.0 bits (77), Expect = 5e-04 Identities = 14/40 (35%), Positives = 25/40 (62%) Query: 249 ARAEGIEQGLERGLERGRAEGIEKGREEGLEQGLERGKAE 288 A +G + G+ G ++G +G ++G +GLEQGL K++ Sbjct: 51 AHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQ 90
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
smi_0031	FLGMOTORFLIM	28	0.034	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 28.3 bits (63), Expect = 0.034 Identities = 18/79 (22%), Positives = 35/79 (44%), Gaps = 7/79 (8%) Query: 119 EEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDI-GYIRTDLPEGFDFT---- 173 EE ++ +P LA+I + LK +L + + F +I+ + G D T Sbjct: 91 EEFIRSIPTPSTLAVITMDPLKGNAVLEVDPSITFSIIDRLFGGTGQAAKVQRDLTDIEN 150 Query: 174 --ISRILERIISGIASHWT 190 + ++ RI++ + WT Sbjct: 151 SVMEGVIVRILANVRESWT 169