PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genome2252.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_004347 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1SO_0084SO_0106Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0084016-3.6352992,3-diketo-5-methylthio-1-phosphopentane
SO_0085023-5.129704predicted inner membrane protein
SO_0086126-6.267401predicted membrane protein
SO_0088122-4.781978predicted periplasmic protein
SO_0090019-3.156048periplasmic protein of unknown function DUF442
SO_0091016-0.360607cyclic nucleotide binding domain protein
SO_0092-1162.200241purine nucleoside phosphorylase DeoD
SO_0093-2163.058608Na+ dependent nucleoside transporter NupC
SO_0095-2204.756813imidazolonepropionase HutI
SO_0096-1234.683178transcriptional repressor of histidine
SO_00970234.788165urocanate hydratase HutU
SO_00980224.716398histidine ammonia-lyase HutH
SO_01011205.073944nitrate-inducible formate dehydrogenase
SO_01021236.024166nitrate-inducible formate dehydrogenase
SO_0103-1225.753515nitrate-inducible formate dehydrogenase
SO_0104-1225.892406nitrate-inducible formate dehydrogenase
SO_0105-1205.070974L-seryl-tRNA selenium transferase SelA
SO_0106-2184.003569selenocysteine-specific translation elongation
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0095UREASE432e-06 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 42.8 bits (101), Expect = 2e-06
Identities = 24/56 (42%), Positives = 32/56 (57%), Gaps = 8/56 (14%)

Query: 348 LAGLTLNAAKALGIEDKVGSLVVGKQADFCLWNIATPAQLAYSYGVNPCKDVVKNG 403
+A T+N A A G+ ++GSL VGK+AD LWN PA +GV P V+ G
Sbjct: 406 IAKYTINPAIAHGLSHEIGSLEVGKRADLVLWN---PAF----FGVKP-DMVLLGG 453



Score = 31.2 bits (71), Expect = 0.008
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 6/61 (9%)

Query: 23 YGAITHAAIAVKDGKIAWLGPRSE---LPAFDVL---SIPVYRGKGGWITPGLIDAHTHL 76
+ I A I +KDG+IA +G P ++ V G+G +T G +D+H H
Sbjct: 80 HWGIVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEGKIVTAGGMDSHIHF 139

Query: 77 V 77
+
Sbjct: 140 I 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0106TCRTETOQM502e-08 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 50.2 bits (120), Expect = 2e-08
Identities = 32/101 (31%), Positives = 48/101 (47%), Gaps = 16/101 (15%)

Query: 6 HVDHGKSTLIRALT---------------GMNTDRLPEEKRRGMTIDLGYAFMPLRDGTR 50
HVD GK+TL +L TD E++RG+TI G + T+
Sbjct: 11 HVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSF-QWENTK 69

Query: 51 LAFIDVPGHEKFINNMLVGVSHVRHALLVLACDDGVMPQTR 91
+ ID PGH F+ + +S + A+L+++ DGV QTR
Sbjct: 70 VNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTR 110


2SO_0375SO_0415Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0375017-3.311014ISSod1 transposase TnpA_ISSod1
SO_4817-114-3.305949hypothetical protein
SO_0377015-4.109454class I glutamine amidotransferase-like domain
SO_0378-113-3.222859ISSod4 transposase TnpA_ISSod4
SO_0379015-3.235293protein of unknown function DUF45
SO_0380-115-3.296681type I restriction-modification system
SO_0381016-3.264650type I RM locus Fic family protein
SO_0382118-4.059786type I restriction-modification system
SO_0383017-1.852934type I restriction-modification system
SO_0384220-3.684723protein of unknown function DUF3296
SO_4831223-3.512609transcriptional regulator AlpA family
SO_0386222-3.360231excisionase/response regulator inhibitor-like
SO_0387224-3.613031hypothetical protein
SO_0388227-3.656236integrase bacteriophage P4 family
SO_0389228-5.140550hypothetical protein
SO_0390124-2.610128excisionase/response regulator inhibitor-like
SO_4762020-1.417995hypothetical protein
SO_0391019-0.406266hypothetical protein
SO_03921180.792724predicted lipoprotein
SO_0393-1153.239055global transcriptional activator Fis
SO_0394-1143.422828tRNA-dihydrouridine synthase DusB
SO_0395-1153.401254ribosomal protein L11 methyltransferase PrmA
SO_03960173.134097quinol:fumarate reductase menaquinol-oxidizing
SO_03970153.040787quinol:fumarate reductase menaquinol-oxidizing
SO_0398-1142.114610quinol:fumarate reductase FAD-binding subunit
SO_0399-211-0.588638quinol:fumarate reductase FeS subunit FrdB
SO_0400010-0.982404putative quinol monooxygenase
SO_0401110-1.050102putative NADPH-dependent quinone oxidoreductase
SO_0402211-1.387723transcriptional regulator LysR family
SO_0403113-1.717202predicted outer membrane protein
SO_0404213-1.579604zinc dependent metalloprotease domain
SO_0405317-1.192827transcription termination factor Rho
SO_0406116-1.336637thioredoxin 1 TrxA
SO_0407014-1.076307ATP-dependent RNA helicase RhlB
SO_0408019-1.066144guanosine pentaphosphate phosphatase GppA
SO_0409119-0.971282thioredoxin family protein
SO_0410216-0.3280077,8-dihydro-8-oxoguanine-triphosphatase MutT
SO_04113200.355472putative zinc-binding protein of unknown
SO_04122180.267168protein of unknown function DUF1342 YacF
SO_04132210.653798dephospho-CoA kinase CoaE
SO_04142190.798166type IV prepilin peptidase PilD
SO_04152190.619343type IV pilus inner membrane protein PilC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0382ALARACEMASE290.029 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 29.4 bits (66), Expect = 0.029
Identities = 12/51 (23%), Positives = 14/51 (27%), Gaps = 7/51 (13%)

Query: 290 GYAEWTNRAIPSLGDVLFTREAPAGESCLVPENTKVCMGQRMVLLRPDANV 340
GYA+ R P+ VL V M V L P
Sbjct: 271 GYADGYPRHAPTGTPVLVDGV-------RTMTVGTVSMDMLAVDLTPCPQA 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4831HTHFIS313e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 3e-04
Identities = 6/22 (27%), Positives = 13/22 (59%)

Query: 24 RITEMCQLLGIDRTTLYRRVKR 45
+ LLG++R TL ++++
Sbjct: 451 NQIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0393DNABINDNGFIS1181e-38 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 118 bits (297), Expect = 1e-38
Identities = 61/101 (60%), Positives = 83/101 (82%), Gaps = 3/101 (2%)

Query: 1 MFDQTTNTEVHQLTVGKIETANGTIKPQLLRDAVKRAVTNFFAQLDGQEAQEVYEMVLSE 60
MF+Q N++V LTV + + + + + LRD+VK+A+ N+FAQL+GQ+ ++YE+VL+E
Sbjct: 1 MFEQRVNSDV--LTVSTVNSQDQVTQ-KPLRDSVKQALKNYFAQLNGQDVNDLYELVLAE 57

Query: 61 VEAPLLDIIMQHTRGNQTRAANMLGINRGTLRKKLKKYGMN 101
VE PLLD++MQ+TRGNQTRAA M+GINRGTLRKKLKKYGMN
Sbjct: 58 VEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN 98


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0414PREPILNPTASE332e-117 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 332 bits (853), Expect = e-117
Identities = 171/303 (56%), Positives = 205/303 (67%), Gaps = 15/303 (4%)

Query: 6 TLLGHTFDQAPWLFISLSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHADIV 65
LL PWL+ SL F+F+ IGSFLNVVIHR P+M++REWQ E Y +
Sbjct: 2 ALLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFNPDDEGVD 61

Query: 66 KQVGIDKLSKAIDHYPPKYNLVVPGSACPKCKTAIKPWHNLPMLGWLMLRGKCAACSAPI 125
P YNL+VP S CP C I N+P+L WL LRG+C C API
Sbjct: 62 ---------------EPPYNLMVPRSCCPHCNHPITALENIPLLSWLWLRGRCRGCQAPI 106

Query: 126 SARYPIIELITGLLVATLAWHFGPSWQFVFAAILTFVLIALTGIDLDEMLLPDQMTLPLL 185
SARYP++EL+T LL +A P W + A +LT+VL+ALT IDLD+MLLPDQ+TLPLL
Sbjct: 107 SARYPLVELLTALLSVAVAMTLAPGWGTLAALLLTWVLVALTFIDLDKMLLPDQLTLPLL 166

Query: 186 WLGLLINLNHTFTSPADAMIGAAAGYLSLWSIFWLFKLLTGKEGMGYGDFKLLAVFGAWL 245
W GLL NL F S DA+IGA AGYL LWS++W FKLLTGKEGMGYGDFKLLA GAWL
Sbjct: 167 WGGLLFNLLGGFVSLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWL 226

Query: 246 GWQMLPLVILLSSLVGAFVGITLIVTKRNQLANPIPFGPYIAAAGWIALIWGQPIVDWYL 305
GWQ LP+V+LLSSLVGAF+GI LI+ + + + PIPFGPY+A AGWIAL+WG I WYL
Sbjct: 227 GWQALPIVLLLSSLVGAFMGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYL 286

Query: 306 STL 308
+
Sbjct: 287 TNF 289


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0415BCTERIALGSPF388e-135 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 388 bits (997), Expect = e-135
Identities = 119/405 (29%), Positives = 213/405 (52%), Gaps = 9/405 (2%)

Query: 25 TFEWKGVNRDGQKTSGELRGASAAEIRSQLKSQGVNP--------KTVRKQSAALFKLGD 76
+ ++ ++ G+K G SA + R L+ +G+ P + S L
Sbjct: 3 QYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLRRK 62

Query: 77 PKITPMDIAMVTRQIATMLAAGVPLVTTIELLGRGHEKVKMRELLATILSEIQSGIPLSD 136
+++ D+A++TRQ+AT++AA +PL ++ + + EK + +L+A + S++ G L+D
Sbjct: 63 IRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLAD 122

Query: 137 ALRPHRRYFDDLYVDLVAAGEHSGSLDVVFDRIATYREKSEALKSKIKKAMFYPAAVVIV 196
A++ F+ LY +VAAGE SG LD V +R+A Y E+ + ++S+I++AM YP + +V
Sbjct: 123 AMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLTVV 182

Query: 197 AILVTALLLLFVVPQFEDIFKGFGAELPAFTQLVLQISRGLQSSWYIFLGAIVAGVFLFV 256
AI V ++LL VVP+ + F LP T++++ +S +++ L A++AG F
Sbjct: 183 AIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMAF- 241

Query: 257 RAHRNSQIVRDRVDEAVLKIPAIGPILHKGAMARFARTLATTFAAGVPLIDGLESAAGAS 316
R + R +L +P IG I AR+ARTL+ A+ VPL+ + +
Sbjct: 242 RVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDVM 301

Query: 317 GNAVYRKAILKIRQEVMAGMQMNVAMRTTGLFPDMLIQMVMIGEESGSLDNMLNKVSTIY 376
N R + V G+ ++ A+ T LFP M+ M+ GE SG LD+ML + +
Sbjct: 302 SNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADNQ 361

Query: 377 EMQVDDAVDGLSSLIEPIMMVVIGTVVGGLIVAMYLPIFQMGKVV 421
+ + + L EP+++V + VV +++A+ PI Q+ ++
Sbjct: 362 DREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLM 406


3SO_0438SO_0454Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0438-1163.728830oxidoreductase short chain
SO_0439-1153.946235hypothetical protein
SO_0440-2153.870839ImpA-like cell surface immunomodulating
SO_0441-2154.138494phosphoribosylamine-glycine ligase PurD
SO_0442-2163.372608bifunctional IMP
SO_04430182.577465zinc and cadmium (II) responsive transcriptional
SO_04441152.171554zinc/cadmium-responsive efflux pump
SO_04452170.981209putative negative regulator of univalent cation
SO_04473170.596424iron-regulated inner membrane protein
SO_04483171.208286iron-regulated inner membrane protein
SO_04493131.705326iron-regulated inner membrane protein
SO_04502121.834256transporter MFS superfamily
SO_04522141.585400thioredoxin 2 TrxC
SO_04531152.174327peptidyl-prolyl cis-trans isomerase FklB
SO_04542152.329522protein of unknown function DUF1850
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0438DHBDHDRGNASE791e-19 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 79.3 bits (195), Expect = 1e-19
Identities = 51/184 (27%), Positives = 82/184 (44%), Gaps = 2/184 (1%)

Query: 3 GLTGKVVIITGASEGIGRALAIAMARIGCQLVLSARNETRLASLALEVANYGPTPFVFAA 62
G+ GK+ ITGA++GIG A+A +A G + N +L + + F A
Sbjct: 5 GIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPA 64

Query: 63 DVSSASQCEDLIHATIAHYGRIDILVNNAGMTMWSRFDELTQLSVLEDIMRVNYLGPAYL 122
DV ++ +++ G IDILVN AG+ L+ E VN G
Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEW-EATFSVNSTGVFNA 123

Query: 123 THAALPYLKSSQ-GQVVIVASVAGLTGVPTRSGYAASKHAVIGFFDSLRIELADDNVAVT 181
+ + Y+ + G +V V S + + YA+SK A + F L +ELA+ N+
Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCN 183

Query: 182 VICP 185
++ P
Sbjct: 184 IVSP 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0450TCRTETA409e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 40.2 bits (94), Expect = 9e-06
Identities = 35/172 (20%), Positives = 59/172 (34%), Gaps = 7/172 (4%)

Query: 215 GLLLGPIYGLLPIYVSQDMGFAQQ---TGQFMALIILGGMIVQPLVSYLSPRIQKSVLMI 271
+ +G I +LP + + G +AL L P++ LS R + ++
Sbjct: 18 AVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRR-PVL 76

Query: 272 AFCLVGAAALLLLMQTSLVGLWLGFV--LLGACAFALYPIAISLACDHLPSSQIVSATQI 329
L GAA +M T+ LW+ ++ ++ A +A + D +
Sbjct: 77 LVSLAGAAVDYAIMATAPF-LWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGF 135

Query: 330 MLLSYSVGSVIGPVAASRFDDIEHGLPLYLAASFLMTACYLSAHLLARSKAR 381
M + G V GPV P + AA+ LL S
Sbjct: 136 MSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKG 187



Score = 30.9 bits (70), Expect = 0.009
Identities = 33/151 (21%), Positives = 64/151 (42%), Gaps = 6/151 (3%)

Query: 12 LFVPVAGLSLFALASGYLMSLIPLSLTYFDLSLDLAP---WLASIFYLGLLLGAPCIAPI 68
L V ++ ++L A+ G +M ++P L S D+ L +++ L AP + +
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 69 VSRIGHSKAFILFLNILLCSVVVMVLLPQTSIWLASRLIAGLAVAGIFVVVESWLLMADT 128
R G ++ L +M P + R++AG+ A V +++
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGA-TGAVAGAYIADITD 125

Query: 129 QKQRAKRLGLYMTALYG-GTAIGQLAVDYLG 158
+RA+ G +M+A +G G G + +G
Sbjct: 126 GDERARHFG-FMSACFGFGMVAGPVLGGLMG 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0453INFPOTNTIATR691e-17 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 68.9 bits (168), Expect = 1e-17
Identities = 38/96 (39%), Positives = 50/96 (52%), Gaps = 2/96 (2%)

Query: 12 GDGKEAVKGALITTQYRGFLQDGTQFDSSYDRGQAFQCVIGTGRVIKGWDQGIMGMKVGG 71
G G + K +T +Y G L DGT FDS+ G+ +VI GW + + M G
Sbjct: 136 GTGAKPGKSDTVTVEYTGTLIDGTVFDSTEKAGKP--ATFQVSQVIPGWTEALQLMPAGS 193

Query: 72 KRKLLVPAHLAYGERQVGAHIKPNSDLTFEIELLEV 107
++ VPA LAYG R VG I PN L F+I L+ V
Sbjct: 194 TWEVFVPADLAYGPRSVGGPIGPNETLIFKIHLISV 229


4SO_4763SO_0486Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_47632141.388201toxin module of toxin-antitoxin system YafO
SO_04673152.436301DNA helicase II UvrD
SO_04681140.7585124-hydroxybenzoate octaprenyltransferase UbiA
SO_4764-1130.467799predicted lipoprotein
SO_0470-1151.150849hypothetical protein
SO_04711121.755963FMN-dependent nitronate monooxygenase
SO_0472213-0.097127inner membrane protein of unknown function
SO_0474216-0.427223protein of unknown function DUF2166
SO_0476216-0.529558cytochrome c maturation system periplasmic
SO_04771160.008384cytochrome c maturation system haem lyase
SO_04782160.151545cytochrome c maturation system haem lyase
SO_0479221-0.032181sulfite reductase octaheme cytochrome c SirA
SO_04800150.804535sulfurtransferase SirB
SO_0481-1151.262809cytochrome c maturation system peptidyl-prolyl
SO_04820151.965481cytochrome c maturation system haem lyase
SO_04830161.6731114Fe-4S ferredoxin SirC
SO_0484-1161.129169menaquinol oxidase SirD
SO_0485-1162.956245ABC-type copper uptake system periplasmic
SO_0486-1173.232317ABC-type copper transport system
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0481INFPOTNTIATR1814e-59 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 181 bits (461), Expect = 4e-59
Identities = 99/227 (43%), Positives = 135/227 (59%), Gaps = 9/227 (3%)

Query: 21 ALFVSMASFAAPSLKTDADKASYSIGASVGNYISGQVYNQVELGAEVNVDLVVQGFVDAL 80
A+ +MA+ A SL TD DK SYSIGA +G Q G ++N D++ +G D +
Sbjct: 14 AMSTAMAATDATSLTTDKDKLSYSIGADLGKNFKNQ-------GIDINPDVLAKGMQDGM 66

Query: 81 K-KQQQLTDEEVVTYLNQRAEELNQVRKANAEKLAAENIKAGEAFLAENKKKAGVTVTES 139
Q LT+E++ L++ ++L R A K A EN G+AFL+ NK K G+ V S
Sbjct: 67 SGAQLILTEEQMKDVLSKFQKDLMAKRSAEFNKKAEENKAKGDAFLSANKSKPGIVVLPS 126

Query: 140 GLQYEVLTKGTGNKPNPEDVVTVEYVGKLIDGTEFENTVGRKEPTRFALMTVIPGWEEGL 199
GLQY+++ GTG KP D VTVEY G LIDGT F++T +P F + VIPGW E L
Sbjct: 127 GLQYKIIDAGTGAKPGKSDTVTVEYTGTLIDGTVFDSTEKAGKPATFQVSQVIPGWTEAL 186

Query: 200 KLMPMGSKYRFVIPANLAYGNEFV-GEIPPQSTLIFEIELKNIEKPS 245
+LMP GS + +PA+LAYG V G I P TLIF+I L +++K +
Sbjct: 187 QLMPAGSTWEVFVPADLAYGPRSVGGPIGPNETLIFKIHLISVKKAA 233


5SO_0506SO_0520Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_05060163.0407623-octaprenyl-4-hydroxybenzoate carboxy-lyase
SO_05081162.909541hypothetical protein
SO_05103182.540210oxidoreductase short-chain
SO_05114171.265284acetyl-CoA carboxylase biotin carboxyl carrier
SO_05124161.7724493-dehydroquinate dehydratase type II AroQ
SO_05134172.619412stop codon-independent peptidyl-tRNA hydrolyzing
SO_05140184.164433protein of unknown function DUF3478
SO_0515-1184.128026DUF3012 domain-containing lipoprotein
SO_0516-1173.997306hypothetical protein
SO_0518-2183.700046heavy metal efflux pump secretin component CzcC
SO_0519-2193.619294heavy metal efflux pump MFP component CzcB
SO_0520-2193.248531heavy metal efflux pump permease component CzcA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0508IGASERPTASE310.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 31.2 bits (70), Expect = 0.004
Identities = 16/78 (20%), Positives = 26/78 (33%)

Query: 143 PTGYDDTPVAISAPVRVTTSMQYSPSEGRMVSNMPSNSATVISAASTARASTVSAEQTVA 202
P +T P + T+S P N ++ + A ++
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSN 1217

Query: 203 VPRARAARSVSSLPSNAR 220
P+ R RSV S+P N
Sbjct: 1218 KPKNRHRRSVRSVPHNVE 1235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0510DHBDHDRGNASE495e-09 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 48.5 bits (115), Expect = 5e-09
Identities = 41/183 (22%), Positives = 80/183 (43%), Gaps = 5/183 (2%)

Query: 2 ILITGASSGLGAALAALYAKDNQALTLTGRDANRLHTVANALSPFSQQAITAIAADLSDE 61
ITGA+ G+G A+A A + + +L V ++L ++ A A AD+ D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHA-EAFPADVRDS 69

Query: 62 ASVEALFDGLTDSPA---TVIHCAGSGYFGALENQGAREIKTLLNNNVTSTILLVRELVK 118
A+++ + + +++ AG G + + E + + N T R + K
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129

Query: 119 RYKNQ-AITVVVVMSTAALAAKAGESTYCAAKWAVRGFIESVRLELKQSPMKLIAVYPGG 177
++ + ++V V S A + + Y ++K A F + + LEL + ++ V PG
Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189

Query: 178 MDT 180
+T
Sbjct: 190 TET 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0511RTXTOXIND280.014 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.3 bits (63), Expect = 0.014
Identities = 9/23 (39%), Positives = 11/23 (47%)

Query: 126 GIIGAIWVKEGDEVAFDQPLFTL 148
I+ I VKEG+ V L L
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKL 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0512TRNSINTIMINR270.022 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 27.4 bits (60), Expect = 0.022
Identities = 11/27 (40%), Positives = 18/27 (66%), Gaps = 2/27 (7%)

Query: 31 DIVAQLNEQAQAAGVQL--EHIQSNAE 55
DIV Q+ +QA+ AG + ++SNA+
Sbjct: 316 DIVEQIAQQAKEAGEVARQQAVESNAQ 342


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0518RTXTOXIND320.004 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.1 bits (73), Expect = 0.004
Identities = 16/160 (10%), Positives = 48/160 (30%), Gaps = 10/160 (6%)

Query: 76 EVQAQIARQQQAELAIAAADRAVYNPEL-GLNYQNSETDTYTVGLSQTLDWGDKRGVATR 134
+ + QA L + EL L + Y +S+ + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 135 LAQLEAQILLADIRLERSQMLAERLLALAEQAQGQKALTFAEQQLRFTQAQLNIAEQRFA 194
+ + Q ++ L++ + AE+ + E R +++L+
Sbjct: 195 FSTWQNQKYQKELNLDKKR---------AERLTVLARINRYENLSRVEKSRLDDFSSLLH 245

Query: 195 AGDLSDVELQLLKLELASNTADYALAEQAALVAEGKVIEL 234
++ + + + + + + E +++
Sbjct: 246 KQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSA 285


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0519RTXTOXIND553e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 54.8 bits (132), Expect = 3e-10
Identities = 29/138 (21%), Positives = 56/138 (40%), Gaps = 9/138 (6%)

Query: 161 EVAKAQAEYINAAAEWSRVRR---MSEGAVSVSRRMQAQVDAELKRAILEAIKMTSEQIR 217
V + + +Y+ A E + E + ++ V K IL+ ++ T++ I
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 218 TLESKPEA----IGSYQLLAPIDGRVQQ-DIAMLGQVFSAGTPLMQLT-DESHLWVEAQL 271
L + + + AP+ +VQQ + G V + LM + ++ L V A +
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 272 TPAQAVNVKVGAPALIQV 289
+ VG A+I+V
Sbjct: 373 QNKDIGFINVGQNAIIKV 390



Score = 42.5 bits (100), Expect = 2e-06
Identities = 26/149 (17%), Positives = 54/149 (36%), Gaps = 5/149 (3%)

Query: 105 SLTNLNLDVRATATLVVDRDKTATLAPQLDVRVQARHVVPGQEVKKGEPLLTLGG----A 160
L + + A L ++ + P + V+ V G+ V+KG+ LL L A
Sbjct: 76 VLGQVEIVATANGKLTHS-GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 161 EVAKAQAEYINAAAEWSRVRRMSEGAVSVSRRMQAQVDAELKRAILEAIKMTSEQIRTLE 220
+ K Q+ + A E +R + +S D + + E + + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 221 SKPEAIGSYQLLAPIDGRVQQDIAMLGQV 249
YQ +D + + + +L ++
Sbjct: 195 FSTWQNQKYQKELNLDKKRAERLTVLARI 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0520ACRIFLAVINRP6560.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 656 bits (1693), Expect = 0.0
Identities = 224/1082 (20%), Positives = 434/1082 (40%), Gaps = 74/1082 (6%)

Query: 9 AIKNRLLVVLALLAAVAASVAMLPKLNLDAFPDVTNVQVTINTEAEGLAAEEVEKLISYP 68
I+ + + + + A + +L + +P + V+++ G A+ V+ ++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 69 VESAMYALPAVTEVRSLS-RTGLSIVTVVFAEGTDIYFARQQVFEQLQAAREMIPSGVGV 127
+E M + + + S S G +T+ F GTD A+ QV +LQ A ++P V
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 128 PEIGPNTSGLGQIYQYILRADPSAGINAAELRSLNDYLVKLILMPVGGVTDVLSFGGEVR 187
I S + +D + G ++ VK L + GV DV FG +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSD-NPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ-Y 182

Query: 188 QYQVQVDPNKLRAYGLSMAQVSEALESNNRNAGGWFMDQGQEQLVVRGYGMLPAGEAGLA 247
++ +D + L Y L+ V L+ N + G L + +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLG-GTPALPGQQLNASIIAQTRFK 241

Query: 248 SIAQIPLTEVR----GTPVRVGDIAKVDFGSEIRVGAVTMTRRDEAGQAQALGEVVAGVV 303
+ + +R G+ VR+ D+A+V+ G E + G+ +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARIN-----GK-----PAAGLGI 291

Query: 304 LKRMGANTKATIDDIDARINLIEQALPKGVSFEVFYDQADLVDKAVATVRDALLMAFVFI 363
GAN T I A++ ++ P+G+ YD V ++ V L A + +
Sbjct: 292 KLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLV 351

Query: 364 VVILALFLVNIRATLLVLLSIPVSIGLALMVMSYYGLSANLMSLGGLAVAIGMLVDGSVV 423
+++ LFL N+RATL+ +++PV + +++ +G S N +++ G+ +AIG+LVD ++V
Sbjct: 352 FLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIV 411

Query: 424 MVENIFKHLTQPDRRHLTQARARADGEADPYHGDEDGGVNADDDDHQGNMAVRIMLAAKE 483
+VEN+ R + + P + +
Sbjct: 412 VVENV--------------ERVMMEDKLPPKEA--------------------TEKSMSQ 437

Query: 484 VCSPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMISALLVALIAVPALAVYLF 543
+ + ++ VF P+ G G +++ +++I+ AM ++LVALI PAL L
Sbjct: 438 IQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLL 497

Query: 544 K----------RGVVLKESVILRPLDNAYRKLLSATLARPKMVVLSAVIMFVMSMALLPR 593
K G + N Y + L +L ++ + L R
Sbjct: 498 KPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLR 557

Query: 594 LGTEFVPELEEGTINLRVTLAPTASLGTSLDVAPKLEALLLQFPEVEYALSRIGAPELGG 653
L + F+PE ++G + L A+ + V ++ L+ + S
Sbjct: 558 LPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVE-SVFTVNGFSF 616

Query: 654 DPEPVSNIEIYIGLKPIEEWQSASSRLA--LQRLMEEKLSVFPGLLLTFSQPIATRVDEL 711
+ + ++ LKP EE + + R E + G ++ F+ P + EL
Sbjct: 617 SGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMP---AIVEL 673

Query: 712 LSGVKAQLA-IKLFGPDLAVLSDKGQVLTDLVAKIPGAV-DVSLEQVSGEAQLVVRPDRS 769
+ I G L+ L + A+ P ++ V + AQ + D+
Sbjct: 674 GTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQE 733

Query: 770 QLARYGISVDQVMTLVSQGIGGASAGQVIDGNARYDINLRLAAEFRSSPDVIKDLLLSGT 829
+ G+S+ + +S +GG ID + ++ A+FR P+ + L +
Sbjct: 734 KAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSA 793

Query: 830 NGAIVRLGEVASVEVEMAPPNIRRDDVQRRVVVQANVA-GRDMGSIVNDIYALVPKADLP 888
NG +V + P + R + + +Q A G G + + L K LP
Sbjct: 794 NGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASK--LP 851

Query: 889 AGYTVIVGGQYENQQRAQQKLMLVVPVSIALIALLLYFSFGAVKQVLLIMANVPLALIGG 948
AG G ++ + + +V +S ++ L L + + + +M VPL ++G
Sbjct: 852 AGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGV 911

Query: 949 IVALFVSGTYLSVPSSIGFITLFGVAVLNGVVLVDSINQ-RRQSGESLYDSVYEGTVGRL 1007
++A + V +G +T G++ N +++V+ + G+ + ++ RL
Sbjct: 912 LLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRL 971

Query: 1008 RPVLMTALTSALGLIPILVSSGVGSEIQKPLAVVIIGGLFSSTALTLLVLPTLYRWLYRR 1067
RP+LMT+L LG++P+ +S+G GS Q + + ++GG+ S+T L + +P + + R
Sbjct: 972 RPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031

Query: 1068 DK 1069
K
Sbjct: 1032 FK 1033



Score = 99.1 bits (247), Expect = 4e-23
Identities = 92/512 (17%), Positives = 192/512 (37%), Gaps = 37/512 (7%)

Query: 575 MVVLSAVIMFVMSMALLPRLGTEFVPELEEGTINLRVTLAPTASLGTSLD-VAPKLEALL 633
VL+ ++M ++A+L +L P + +++ P A T D V +E +
Sbjct: 12 AWVLAIILMMAGALAIL-QLPVAQYPTIAPPAVSVSAN-YPGADAQTVQDTVTQVIEQNM 69

Query: 634 LQFPEVEYALSRIGAPELGGDPEPVSNIEIYIGLKPIEEWQSASSRLALQRLMEEKLSVF 693
+ Y S + ++ I + + +A ++ KL +
Sbjct: 70 NGIDNLMYMSST---------SDSAGSVTITLTFQS-----GTDPDIAQVQVQN-KLQLA 114

Query: 694 PGLLLTFSQPIATRVDELLSGVKAQLAIKLFGPDL--AVLSDKGQ-VLTDLVAKIPGAVD 750
LL Q V++ S P +SD + D ++++ G D
Sbjct: 115 TPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGD 174

Query: 751 VSLEQVSGEAQLVVRPDRSQLARYGISVDQVMTLVSQGIGGASAGQVIDGNARYDINLRL 810
V L + + + D L +Y ++ V+ + +AGQ+ A L
Sbjct: 175 VQL--FGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNA 232

Query: 811 A----AEFRSSPDVIKDLLLSGTNGAIVRLGEVASVEVEMAPPNIR-RDDVQRRVVVQAN 865
+ F++ + K L ++G++VRL +VA VE+ N+ R + + +
Sbjct: 233 SIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIK 292

Query: 866 VA-GRDMGSIVNDIYALVP--KADLPAGYTVIVGGQYENQQRAQQKLMLVVP---VSIAL 919
+A G + I A + + P G V+ Y+ Q + VV +I L
Sbjct: 293 LATGANALDTAKAIKAKLAELQPFFPQGMKVLY--PYDTTPFVQLSIHEVVKTLFEAIML 350

Query: 920 IALLLYFSFGAVKQVLLIMANVPLALIGGIVALFVSGTYLSVPSSIGFITLFGVAVLNGV 979
+ L++Y ++ L+ VP+ L+G L G ++ + G + G+ V + +
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAI 410

Query: 980 VLVDSINQRRQS-GESLYDSVYEGTVGRLRPVLMTALTSALGLIPILVSSGVGSEIQKPL 1038
V+V+++ + ++ + ++ A+ + IP+ G I +
Sbjct: 411 VVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQF 470

Query: 1039 AVVIIGGLFSSTALTLLVLPTLYRWLYRRDKR 1070
++ I+ + S + L++ P L L +
Sbjct: 471 SITIVSAMALSVLVALILTPALCATLLKPVSA 502


6SO_0602SO_0608Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0602220-0.429194tRNA dimethylallyltransferase MiaA
SO_0603324-0.597033RNA-binding pleiotrophic regulator Hfq
SO_0604323-0.65279450S ribosome assembly GTPase HflX
SO_0605327-1.267683membrane anchored FtsH modulator component 1
SO_0606328-1.631771membrane anchored FtsH modulator component 2
SO_0608226-0.829696ubiquinol-cytochrome c reductase FeS subunit
7SO_0643SO_0678Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0643-1213.278895Mu phage transposase OrfA TnpA_MuSo1a
SO_06440212.953188Mu phage transposase OrfB TnpA_MuSo1b
SO_06450233.424086Mu phage uncharacterized protein
SO_06460263.577221Mu Lambda phage repressor-like DNA binding
SO_06471293.150615Mu phage protein Kil
SO_0648020-0.388491Mu phage protein of unknown function DUF3164
SO_0649120-2.068633Mu phage uncharacterized protein
SO_0650121-3.867021Mu phage uncharacterized protein
SO_0651024-3.783251Mu phage host gene modulation protein GemA
SO_0652124-4.018355Mu phage middle operon regulator Mor
SO_0653123-3.542605Mu phage uncharacterized protein
SO_0654221-0.725603Mu phage uncharacterized protein
SO_06553200.244590Mu phage protein
SO_06563201.920079ISSod1 transposase TnpA_ISSod1
SO_06585193.437105Mu phage uncharacterized protein E18
SO_06595192.983192Mu phage lysozyme
SO_06604173.005326Mu phage conserved phage protein
SO_06611193.529435Mu phage mom transcriptional regulator Com
SO_0662-1214.718524Mu phage protein of unknown function DUF2730
SO_0663-1205.418757Mu phage uncharacterized protein Gp26
SO_0664-1225.685348Mu phage uncharacterized protein
SO_0665-1236.055492Mu phage small terminase subunit GpD
SO_0666-1214.344612Mu phage large terminase subunit GpE
SO_06671203.758987Mu phage portal protein
SO_06681200.612654Mu phage minor capsid protein GpF
SO_4832428-3.880565Mu phage protein
SO_0669122-1.660473Mu phage tail completion protein GpG
SO_0670221-1.624304Mu phage uncharacterized protein
SO_0671122-0.073093Mu phage uncharacterized protein
SO_06721220.739653Mu phage signal peptidase I domain protein
SO_06731211.889925Mu phage uncharacterized protein
SO_06741214.755926Mu phage peptidase
SO_06753234.658278Mu phage major head subunit
SO_06763224.590386Mu phage uncharacterized protein
SO_06771204.186896Mu phage protein of unknown function DUF1320
SO_06781183.256967Mu phage uncharacterized protein
8SO_0703SO_0710Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0703022-3.84816210 kDa chaperonin GroES
SO_0704-122-4.12679560 kDa chaperonin GroEL
SO_0705-117-5.794975toxin-antitoxin system antidote transcriptional
SO_0706-115-4.302854toxin-antitoxin system toxin HipA family
SO_0708-116-3.970555ISSod18 transposase TnpA_ISSod18
SO_0710018-5.324315P-loop containing nucleoside triphosphate
9SO_0773SO_0795Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_07731173.855988ISSod6 transposase TnpA_ISSod6
SO_07740184.9966935-formyltetrahydrofolate cyclo-ligase YgfA
SO_07750204.771743cell division protein interacting with FtsZ
SO_07761284.857178YecA family protein
SO_07770263.0454222-octaprenyl-6-methoxyphenol hydroxylase UbiH
SO_0778-122-1.123448FAD-dependent hydroxylase VisC
SO_0779126-3.616756aminomethyltransferase GcvT
SO_0780127-5.202559glycine cleavage system carrier of aminomethyl
SO_0781026-5.536615glycine dehydrogenase (decarboxylating) GcvP
SO_0782333-11.095551hypothetical protein
SO_0783227-8.626376superfamily I DNA and RNA helicase
SO_0786112-2.105785hypothetical protein
SO_0787114-0.906451hypothetical protein
SO_07881160.289737protein of unknown function DUF3297
SO_07891170.906231short-chain dehydrogenase/reductase family
SO_07922170.901178ribosome recycling factor Rrf
SO_07952181.441730protein involved in RimO-mediated
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0789DHBDHDRGNASE448e-08 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 44.3 bits (104), Expect = 8e-08
Identities = 37/177 (20%), Positives = 62/177 (35%), Gaps = 25/177 (14%)

Query: 3 ILVVGAAGNIGQAVTRLLKAEGHQVIQV-----------------GRTRGDLLMDICDPN 45
+ GAA IG+AV R L ++G + V R D+ D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70

Query: 46 SLQQGFNQL----GKVDAIIAAMGDVAFKPFVQLDQADWQKGIQSKLLGQIQLVQIGSQF 101
++ + ++ G +D ++ G + L +W+ G + S++
Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130

Query: 102 LNA--GGSFTLTSGIIADVPVKDGVSAATINGALEHFVAAVANELPQQ--RINIVSP 154
+ GS A VP + A+ A F + EL + R NIVSP
Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187


10SO_0831SO_0844Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_08312161.873026ATP-dependent glutathione synthetase GshB
SO_08320151.40468616S rRNA (uracil1498-N3)-methyltransferase RsmE
SO_0833-1160.386278endonuclease I EndA
SO_0834013-0.142997protein of unknown function DUF335 SprT
SO_0835222-0.289777putative periplasmic peptidase C15 superfamily
SO_0837323-0.645468class D carbapenem-hydrolyzing beta-lactamase
SO_0839325-0.675747transcriptional regulator LysR family
SO_0840327-0.703594acetyl-CoA carboxylase multifunctional enzyme
SO_0841120-0.308127bifunctional periplasmic substrate binding
SO_08421251.411287translation elongation factor G FusA-like
SO_0843-1153.058069transcriptional regulator LysR family
SO_0844-1173.388355hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0842TCRTETOQM5500.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 550 bits (1420), Expect = 0.0
Identities = 185/689 (26%), Positives = 300/689 (43%), Gaps = 70/689 (10%)

Query: 6 KYRNIGIFAHVDAGKTTTTERILKLTGKIHKIGEVHDGESTTDFMVQEAERGITIQSAAV 65
K NIG+ AHVDAGKTT TE +L +G I ++G V G + TD + E +RGITIQ+
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 66 SCFWKDHRFNVIDTPGHVDFTVEVYRSLKVLDGGIAVFCGSGGVEPQSETNWRYANESEV 125
S W++ + N+IDTPGH+DF EVYRSL VLDG I + GV+ Q+ + + +
Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 126 ARIIFVNKLDRMGADFLRVVKQTKDVLAANPLVMVLPIGIEDEFCGVVDLLTRKAYVWDD 185
I F+NK+D+ G D V + K+ L+A ++
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ------------------------- 156

Query: 186 SGIPENFEVKDVPANMVDLVEEYREMLIETAVEQDDDLLEAYMEGEEPSIEDLKRCIRKG 245
+V+ P V E + +T +E +DDLLE YM G+ +L++
Sbjct: 157 -------KVELYPNMCVTNFTESEQ--WDTVIEGNDDLLEKYMSGKSLEALELEQEESIR 207

Query: 246 TRTMAFFPTFCGSAFKNKGMQLVLDAVVDYLPAPDEVDPQPLTDEEGNETGEYAIVSADE 305
+ FP + GSA N G+ +++ + + + T +E
Sbjct: 208 FHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSS--------THRGQSE----------- 248

Query: 306 SLKALAFKI-MDDRFGALTFVRIYAGRLKKGDTILNSATGKTERIGRMCEMYANDRIEIE 364
L FKI ++ L ++R+Y+G L D++ S K +I M + +I+
Sbjct: 249 -LCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKID 306

Query: 365 SAEAGDIIAIVGMKNVQTGHTLCDVKHPCTLEAMVFPEPVISIAVAPKDKGGSEKMAIAI 424
A +G+I+ + + ++ L D K E + P P++ V P E + A+
Sbjct: 307 KAYSGEIVILQN-EFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDAL 365

Query: 425 GKMIAEDPSFRVETDEDSGETILKGMGELHLDIKVDILKRTYGVELIVGEPQVAYRETIT 484
++ DP R D + E IL +G++ +++ +L+ Y VE+ + EP V Y E
Sbjct: 366 LEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYMERPL 425

Query: 485 AMVEDQYTHKKQSGGSGQFGKIEYIIRPGEPNSGFVFKSSVVGGSVPKEFWPAVEKGFAS 544
E YT + + + I + P SG ++SSV G + + F AV +G
Sbjct: 426 KKAE--YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRY 483

Query: 545 MMNTGTIAGFPVLDVEFELTDGAYHAVDSSAIAFEIAAKAAFRQSIAKAKPQLLEPIMKV 604
G + G+ V D + G Y++ S+ F + A Q + KA +LLEP +
Sbjct: 484 GCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSF 542

Query: 605 DVFSPDDNVGDVIGDLNRRRGMIKDQVAGITGVRVKADVPLSEMFGYIGSLRTMTSGRGQ 664
+++P + + D + I D V + ++P + Y L T+GR
Sbjct: 543 KIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSV 602

Query: 665 FSMEFSHYSPC----------PNSVADKV 683
E Y PNS DKV
Sbjct: 603 CLTELKGYHVTTGEPVCQPRRPNSRIDKV 631


11SO_1003SO_1035Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_10030243.657598outer membrane morn variant repeat-containing
SO_10042356.676474putative inner membrane lipoprotein
SO_100654810.505326dienelactone hydrolase family protein YghX
SO_100765211.214086lysine transporter LysW
SO_1008105812.320166ISSod20 transposase TnpA_ISSod20
SO_1009126614.417241NADH-ubiquinone oxidoreductase subunit N NuoN
SO_1010106413.567241NADH-ubiquinone oxidoreductase subunit M NuoM
SO_101155613.428287NADH-ubiquinone oxidoreductase subunit L NuoL
SO_101235212.860465NADH-ubiquinone oxidoreductase subunit K NuoK
SO_101335112.710815NADH-ubiquinone oxidoreductase subunit J NuoJ
SO_101435512.752098NADH-ubiquinone oxidoreductase subunit I NuoI
SO_101535412.766086NADH-ubiquinone oxidoreductase subunit H NuoH
SO_101625212.541443NADH-ubiquinone oxidoreductase subunit G NuoG
SO_10171459.155954NADH-ubiquinone oxidoreductase subunit F NuoF
SO_10180345.491514NADH-ubiquinone oxidoreductase subunit E NuoE
SO_10190324.506935NADH-ubiquinone oxidoreductase subunit CD NuoCD
SO_1020-119-0.106396NADH-ubiquinone oxidoreductase subunit B NuoB
SO_1021018-3.996541NADH-ubiquinone oxidoreductase subunit A NuoA
SO_10250160.610920ISSod1 transposase TnpA_ISSod1
SO_10261171.993180ISSod4 transposase TnpA_ISSod4
SO_10271193.028723predicted periplasmic protein
SO_10281193.234207hypothetical protein
SO_10291193.645157predicted lipoprotein
SO_10301214.389759B12-dependent
SO_10314223.478273alpha-ribazole-5-phosphate phosphatase CobC
SO_10333222.914702ABC-type cobalamin uptake system ATPase
SO_10342212.935737ABC-type cobalamin uptake system permease
SO_10352181.472307nicotinate-nucleotide--dimethylbenzimidazole
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1030BCTERIALGSPD310.026 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 31.4 bits (71), Expect = 0.026
Identities = 14/71 (19%), Positives = 31/71 (43%), Gaps = 5/71 (7%)

Query: 354 AGLEPLTIDAQTLFVNVGERTN---VTGSAKFLKLIKEGKFEQALDVAREQVESGAQIID 410
+P+ + + + +TN VT + + ++ + LD+ R QV A I +
Sbjct: 298 QAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLE--RVIAQLDIRRPQVLVEAIIAE 355

Query: 411 INMDEGMLDGV 421
+ +G+ G+
Sbjct: 356 VQDADGLNLGI 366


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1031RTXTOXINA290.022 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 28.8 bits (64), Expect = 0.022
Identities = 15/70 (21%), Positives = 25/70 (35%), Gaps = 4/70 (5%)

Query: 159 AIAAILEQAFACLPLGDKPADAAADTAANIWVVTH--GGVIRHLMARALGAVKAVGFYSQ 216
++ IL A L + AD AA + + T G V + + + A G
Sbjct: 244 TVSGILSAISASFILSNADADTRTKAAAGVELTTKVLGNVGKGISQYIIAQRAAQGL--S 301

Query: 217 LTLPVAALVT 226
+ A L+
Sbjct: 302 TSAAAAGLIA 311


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1033PF05272300.012 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.012
Identities = 11/54 (20%), Positives = 20/54 (37%), Gaps = 4/54 (7%)

Query: 29 DVALNVSQLSWTIEGKTILSGVNFALQRG----EMLGLIGPNGAGKSSLLRCLY 78
D + + ++ V ++ G + L G G GKS+L+ L
Sbjct: 564 DYKPRRLRYLQLVGKYILMGHVARVMEPGCKFDYSVVLEGTGGIGKSTLINTLV 617


12SO_1123SO_1139Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_11232200.217222predicted inner membrane protein
SO_11243180.031156aminoacyl-tRNA deacylase YbaK
SO_1125418-1.03133310 TMS drug/metabolite efflux pump (DME) family
SO_11263170.033067chaperone protein DnaK
SO_11270150.729784chaperone protein DnaJ
SO_1130119-0.218037ISSod1 transposase TnpA_ISSod1
SO_1133-1160.663531ISSod1 transposase TnpA_ISSod1
SO_1135-2141.280572protein of unknown function DUF3037
SO_11361223.061781ATP-dependent RNA helicase DEAD box family
SO_11372212.457659Zn-dependent peptidase M48 family
SO_11393221.285232peptidyl-prolyl cis-trans isomerase FklB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1126SHAPEPROTEIN1406e-39 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 140 bits (354), Expect = 6e-39
Identities = 80/386 (20%), Positives = 146/386 (37%), Gaps = 81/386 (20%)

Query: 5 IGIDLGTTNSCVAVLDGGK-----ARVLENAEGDRTTPSIIAYTDDETIVGQPAKRQAVT 59
+ IDLGT N+ + V G + V + + S+ A VG AK+
Sbjct: 13 LSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA-------VGHDAKQMLGR 65

Query: 60 NPNNTFFAIKRLIGRRFKDDEVQRDVNIMPFKIIAADNGDAWVESRGNKMAPPQVSAEIL 119
P N AI+ + +D I F V+ ++L
Sbjct: 66 TPGN-IAAIRPM-----------KDGVIADFF----------------------VTEKML 91

Query: 120 KK-MKKTAEDFLGEEVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAAALAY 178
+ +K+ + ++ VP +R+A +++ + AG +I EP AAA+
Sbjct: 92 QHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGA 151

Query: 179 GIDKKQGDNIVAVYDLGGGTFDISIIEIDSNDGDQTFEVLATNGDTHLGGEDFDNRLINY 238
G+ + + V D+GGGT ++++I ++ + + +GG+ FD +INY
Sbjct: 152 GLPVSEATGSM-VVDIGGGTTEVAVISLNG---------VVYSSSVRIGGDRFDEAIINY 201

Query: 239 LADEFKKEQGLDLRKDPLAMQRLKEAAEKAKIELSST----NQTEVNLPYITADATGPKH 294
+ + G + AE+ K E+ S E+ + P+
Sbjct: 202 VRRNYGSLIG-------------EATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRG 248

Query: 295 LVVKITRAKLESLVEDLIIRTLEPLKVALADA--DLSVSDINE--VILVGGQTRMPKVQE 350
+ + LE+L E + + + VAL +L+ SDI+E ++L GG + +
Sbjct: 249 FTLN-SNEILEALQEP-LTGIVSAVMVALEQCPPELA-SDISERGMVLTGGGALLRNLDR 305

Query: 351 AVTNFFGKEPRKDVNPDEAVAVGAAI 376
+ G +P VA G
Sbjct: 306 LLMEETGIPVVVAEDPLTCVARGGGK 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1139INFPOTNTIATR1609e-52 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 160 bits (407), Expect = 9e-52
Identities = 79/205 (38%), Positives = 124/205 (60%), Gaps = 9/205 (4%)

Query: 6 STVEQQASYGVGRQMGEQLAANSFEGVDI-PAVQA-GLADAFAGLESAVSMQELQVAFTE 63
+T + + SY +G +G+ +G+DI P V A G+ D +G + ++ ++++ ++
Sbjct: 28 TTDKDKLSYSIGADLGKNFKN---QGIDINPDVLAKGMQDGMSGAQLILTEEQMKDVLSK 84

Query: 64 ISRRIQAAQ----EQAAAEASAEGEAFLVENANREGVIVTESGLQYEVLVQGNGAKPTYE 119
+ + A + + A E A+G+AFL N ++ G++V SGLQY+++ G GAKP
Sbjct: 85 FQKDLMAKRSAEFNKKAEENKAKGDAFLSANKSKPGIVVLPSGLQYKIIDAGTGAKPGKS 144

Query: 120 DTVRTHYHGSFINGDVFDSSVVRGQPAEFPVSGVIAGWTEALQLMPVGTKLKLYVPHHLA 179
DTV Y G+ I+G VFDS+ G+PA F VS VI GWTEALQLMP G+ +++VP LA
Sbjct: 145 DTVTVEYTGTLIDGTVFDSTEKAGKPATFQVSQVIPGWTEALQLMPAGSTWEVFVPADLA 204

Query: 180 YGERGAGASIPPYSTLVFEVELLDI 204
YG R G I P TL+F++ L+ +
Sbjct: 205 YGPRSVGGPIGPNETLIFKIHLISV 229


13SO_1404SO_1448Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_14042130.669534endoribonuclease L-PSP
SO_14052140.395481periplasmic transglutaminase family protein
SO_14062170.679163Hg(II) uptake system permease component MerT
SO_14071190.047745Hg(II) uptake system substrate-binding component
SO_14082190.213005helicase
SO_14091250.419278SAM-dependent methyltransferase
SO_1410221-0.622490periplasmic RmlC-type Cupin domain family
SO_1411119-0.408109predicted outer membrane protein
SO_1412017-0.823503outer membrane beta barrel protein
SO_1413015-1.970519flavocytochrome c heme submit
SO_1414011-2.689292flavocytochrome c flavin subunit
SO_1415114-4.135401transcriptional repressor of flavocytochrome c
SO_1416216-3.940743two component signal transduction system
SO_1417218-4.964608two component signal transduction system
SO_1418118-4.959537ApbE family protein
SO_1419015-3.887814predicted FMN-binding domain-containing
SO_1420016-3.534904metal-induced outer membrane porin IfcO family
SO_1421-114-2.543322periplasmic tetraheme flavocytochrome IfcA
SO_1422-113-1.729085Fe(III)-induced transcriptional regulator IfcR
SO_1424012-0.964811predicted outer membrane lipoprotein
SO_1425013-1.227199outer membrane morn variant repeat-containing
SO_1427015-1.632714periplasmic decaheme cytochrome c DmsE
SO_1428-114-2.655170extracellular dimethyl sulfoxide/manganese oxide
SO_1429-121-4.065162extracellular dimethyl sulfoxide/manganese oxide
SO_1430021-5.769893extracellular dimethyl sulfoxide/manganese oxide
SO_1431120-5.569872extracellular dimethyl sulfoxide/manganese oxide
SO_1432124-7.296511extracellular dimethyl sulfoxide/manganese oxide
SO_1434124-6.898800chemotaxis signal transduction system methyl
SO_1436228-7.832850ISSod11 transposase TnpA_ISSod11
SO_1438227-8.050230ISSod4 transposase TnpA_ISSod4
SO_1440229-8.473187bifunctional toxin-antitoxin system HepN family
SO_1441131-9.083266putative cytoplasmic protein
SO_4821123-4.873708Mu phage lytic protein AlpA
SO_1442020-3.416352hypothetical protein
SO_14433200.873738protein of unknown function DUF3296 YagK
SO_14445283.281569toxin module of toxin-antitoxin system RelE/StbE
SO_14454283.506041transcriptional regulator CopG family
SO_14473293.356203retinol acyltransferase domain protein
SO_14482313.639697site-specific recombinase phage integrase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1415HTHTETR518e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 50.8 bits (121), Expect = 8e-10
Identities = 28/169 (16%), Positives = 57/169 (33%), Gaps = 12/169 (7%)

Query: 15 QILDAAEKLIESQGVVSFKFSQLAHEVGCSTGTLYKFFERKEDVLVCLFLR-----SATS 69
ILD A +L QGV S ++A G + G +Y F+ K D+ ++
Sbjct: 15 HILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELE 74

Query: 70 NHLPIFIHKNPELTAQEKVLLPILFTFETIKRSSSFFTLRSVSVNTMVWKLASDEKVERF 129
+P +E ++ + T +R + V++
Sbjct: 75 LEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEM-----AVVQQA 129

Query: 130 KKRIN-AFWSWFTDSLHLAVENGELVATPLQIKELVQGITFYLTGSLTQ 177
++ + + +L +E L A L + + Y++G +
Sbjct: 130 QRNLCLESYDRIEQTLKHCIEAKMLPA-DLMTRRAAIIMRGYISGLMEN 177


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1416HTHFIS983e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 98.0 bits (244), Expect = 3e-26
Identities = 24/120 (20%), Positives = 58/120 (48%)

Query: 4 LYLVDDDQDVLDSLSWMLEGMGLNCKGFNAADAFLKSVDIKQPAVLLLDIKMPGMDGVAL 63
+ + DDD + L+ L G + + + A + + +++ D+ MP + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 64 LNHLNQAQSAISVIMLTGHGTIAMAVDCIQQGALNFLEKPVDGEKLFQLLTQAQQHTEQK 123
L + +A+ + V++++ T A+ ++GA ++L KP D +L ++ +A +++
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1420ECOLNEIPORIN692e-15 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 69.4 bits (170), Expect = 2e-15
Identities = 87/358 (24%), Positives = 129/358 (36%), Gaps = 40/358 (11%)

Query: 1 MNKSFAISLLTLALCATQAVADEHKFYGRIDYSVTHSDS----GSATHKNKSGTILENNF 56
M KS L A A YG I V S S G+ ++GT + +
Sbjct: 1 MKKSLIALTLAALPVAAMADVT---LYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLG 57

Query: 57 SRFGIKGSSQLTDSTSLFYQIEVGVNGESQDSGDKPFSARPTFIGVKHSTYGALAVGRID 116
S+ G KG L + +Q+E + DSG R +FIG+K +G L VGR++
Sbjct: 58 SKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWG---NRQSFIGLK-GGFGKLRVGRLN 113

Query: 117 PVFKMAKGMSDAMDNYSLKHDRLFAGDKRWGDSFEYKSAHWNKLQLGVSYLLEDNHYSNN 176
V K ++ A + S Y S + L V Y L DN +
Sbjct: 114 SVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGSVQYALNDN--AGR 171

Query: 177 DNRRDNG---NYQ---LAVTYGDKFFKTSDVYAAVAYSDGVEDIEAYRGVIQYKWDKW-- 228
N NY+ V YG + + V V +E + +R V Y D
Sbjct: 172 HNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQENV----NIEKYQIHRLVSGYDNDALYA 227

Query: 229 QFATMLQHSQLVNTDKTDWQQREGDGVIVSAKYQLGQLSLNAQYGFDNSGTGLIANRIYA 288
A Q ++LV + + Q E V + Y+ G ++ Y G +
Sbjct: 228 SVAVQQQDAKLVEENYSHNSQTE---VAATLAYRFGNVTPRVSY-----AHGFKGSFDAT 279

Query: 289 SKNTLIDEVPEISQWAIGAEYKLSKSTRLHTEIGQFDVKQY-DDFDDTIVSLGMRYDF 345
+ N D+V +GAEY SK T G + F T +G+R+ F
Sbjct: 280 NYNNDYDQV------VVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1424ACETATEKNASE320.012 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 31.7 bits (72), Expect = 0.012
Identities = 13/67 (19%), Positives = 26/67 (38%), Gaps = 6/67 (8%)

Query: 121 GSVKEFSALNKPLWQAQLAKRGIEVEQELKKLFSSSAFINTIPAKVGAVVTLPAYQGHTN 180
+ E + L G ++++E K+ A I+T +KV +V TN
Sbjct: 330 AGIGENGPEIREFILDGLEFLGFKLDKEKNKVRGEEAIISTADSKVNVMVV------PTN 383

Query: 181 AKLTVLQ 187
+ + +
Sbjct: 384 EEYMIAK 390


14SO_1459SO_1474Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1459017-3.025648concanavalin A-like lectin/glucanase domain
SO_1460117-3.094001type I restriction-modification system
SO_1461016-3.166076serine/threonine protein kinase
SO_1462225-4.171330cytoplasmic protein in type I
SO_1464225-3.446632ISSod1 transposase TnpA_ISSod1
SO_1468221-3.698794ADP-ribose binding domain-containing protein
SO_1469220-3.871338transcriptional regulator Cro/CI family
SO_4773120-3.349382predicted membrane protein
SO_1470016-2.310885hypothetical protein
SO_1471115-1.786670CP4-57-like prophage integrase IntA
SO_1473216-1.152302SsrA-binding protein SmpB
SO_1474-121-3.009434lipid binding/transport family protein YfjG
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1461YERSSTKINASE383e-04 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 38.2 bits (88), Expect = 3e-04
Identities = 28/88 (31%), Positives = 45/88 (51%), Gaps = 11/88 (12%)

Query: 303 KRIVNDLLLALAHLCRNGVVHRNITPEHILMG-ADGQPRLIDFD-YARIGAENTTTIADE 360
K I + LL HL + GVVH +I P +++ A G+P +ID ++R G E
Sbjct: 248 KFIAHRLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSG---------E 298

Query: 361 VQQRISNRYKAPELWADSHAASCATDIY 388
+ + +KAPEL + AS +D++
Sbjct: 299 QPKGFTESFKAPELGVGNLGASEKSDVF 326



Score = 32.0 bits (72), Expect = 0.022
Identities = 30/119 (25%), Positives = 54/119 (45%), Gaps = 18/119 (15%)

Query: 544 LERLKQEYRTLVKLPEHPYVVKVYDADVLP--NQGPPYIVFEYLEGLDVSELI------- 594
LE K Y+T K HP + V+ V+P N+ ++ + ++G S+ +
Sbjct: 177 LEAYKHIYKTAGK---HPNLANVHGMAVVPYGNRKEEALLMDEVDGWRCSDTLRTLADSW 233

Query: 595 QQRSLTAHEVWTMAKQVAEGL----QHLHEHNIFHCDIKPQNLMWK--DGKVRIIDFNV 647
+Q + + W K +A L HL + + H DIKP N+++ G+ +ID +
Sbjct: 234 KQGKINSEAYWGTIKFIAHRLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGL 292


15SO_1586SO_1602Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1586018-3.085244cyclic nucleotide-binding domain protein
SO_1587115-0.288220predicted membrane protein
SO_15881162.980096hypothetical protein
SO_15892152.976239hypothetical protein
SO_15902164.022590protein of unknown function DUF3224
SO_15952163.905736hypothetical protein
SO_15972174.451789omega-3 polyunsaturated fatty acid synthase
SO_15993164.202258multi-domain beta-ketoacyl synthase PfaC
SO_16002143.155100omega-3 polyunsaturated fatty acid synthase
SO_16020133.040121omega-3 polyunsaturated fatty acid synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1602PF03544330.008 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 33.4 bits (76), Expect = 0.008
Identities = 20/85 (23%), Positives = 28/85 (32%), Gaps = 2/85 (2%)

Query: 1141 PASQVQAPIQAAA-PVAVAVTKPVVPAQAPVVQGLAAEPKVTAVPVSEPTVQQPQVALAQ 1199
P VQ P + P P P +APVV P V+QP+ +
Sbjct: 62 PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK-PKPVKKVEQPKRDVKP 120

Query: 1200 VAQTKVTQPPLAQPQVQTVAAQTSA 1224
V + P T + T+A
Sbjct: 121 VESRPASPFENTAPARPTSSTATAA 145


16SO_1613SO_1629Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1613216-3.114156protein of unknown function DUF2789
SO_1614216-2.705268acteyltransferase GNAT family
SO_1615115-2.181498inner membrane protein of unknown function
SO_1616115-2.338185ISSod4 transposase TnpA_ISSod4
SO_1617215-1.537281protein of unknown function DUF3301
SO_1618116-0.211863protein of unknown function DUF3549
SO_1619018-0.030431tRNA pseudouridine synthase C-associated protein
SO_16200150.168674tRNA pseudouridine65 synthase TruC
SO_16210130.793488hypothetical protein
SO_16220120.750007tRNA pseudouridine synthase C-associated
SO_16230110.790396phosphotransferase system (PTS) glucose-specific
SO_16242200.245050formyltetrahydrofolate deformylase PurU
SO_16252240.6199912,3,4,5-tetrahydropyridine-2,6-carboxylate
SO_16263230.302191protein-P-II uridylyltransferase GlnD
SO_1627331-0.577229methionine aminopeptidase Map
SO_1629227-0.57394530S ribosomal protein S2 RpsB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1614SACTRNSFRASE280.016 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.4 bits (63), Expect = 0.016
Identities = 14/53 (26%), Positives = 25/53 (47%), Gaps = 3/53 (5%)

Query: 138 IALAPNEQGKGLGHQLVQAVVSWCDEQPNLEGIGVFTTQE--AHTHLFKQHDF 188
IA+A + + KG+G L+ + W E G+ + T + H + +H F
Sbjct: 95 IAVAKDYRKKGVGTALLHKAIEWAKENH-FCGLMLETQDINISACHFYAKHHF 146


17SO_1674SO_1679Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1674-1153.809346short chain dehydrogenase family protein
SO_1675-2164.188659predicted lipoprotein
SO_1676-2154.084677homoserine O-succinyltransferase MetA
SO_1677-2154.5327293-ketoacyl-CoA thiolase IvdA
SO_1678-2164.254125methylmalonate-semialdehyde dehydrogenase IvdB
SO_1679-1143.3854782-methylbutanoyl-CoA dehydrogenase IvdC
18SO_1691SO_1711Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1691220-2.950858lipocalin family lipoprotein Blc
SO_1692117-1.73730910 TMS drug/metabolite efflux pump (DME) family
SO_1694118-0.682903FAD-binding protein
SO_16951201.085672diguanylate cyclase with PAS sensory domain
SO_16971200.892454protein of unknown function DUF1568
SO_16983192.376131autocatalytic aspartic peptidase
SO_16993203.450545transmembrane transcriptional regulator
SO_17002235.087552putative lipoprotein
SO_17013224.095001secreted protein of unknown function
SO_17022243.347012hypothetical protein
SO_17050191.655233ABC-type drugE1 family efflux system MFP
SO_1706-2150.023033ABC-type drugE1 family efflux system ATPase
SO_1707-214-1.059512ABC-type drugE1 family efflux system permease
SO_1708-313-2.088532acetyl-CoA hydrolase/transferase
SO_1711-217-3.216064hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1691BCTLIPOCALIN2544e-90 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 254 bits (651), Expect = 4e-90
Identities = 112/171 (65%), Positives = 145/171 (84%)

Query: 1 MKKLLLMISVLVLTGCLGMPNYVEPVKDFELDRYLGKWYEIARLDHSFERGLTQVTAEYS 60
M+ + L++ ++L GCLGMP V+PV DFEL+ YLGKWYE+ARLDHSFERGL+QVTAEY
Sbjct: 1 MRAIFLILCSVLLNGCLGMPESVKPVSDFELNNYLGKWYEVARLDHSFERGLSQVTAEYR 60

Query: 61 LKPDGGVKVINRGYSAAKQQWKEAEGKAYFVNGENEAYLKVSFFGPFYGAYVVFGLDQQN 120
++ DGG+ V+NRGYS K +WKEAEGKAYFVNG + YLKVSFFGPFYG+YVVF LD++N
Sbjct: 61 VRNDGGISVLNRGYSEEKGEWKEAEGKAYFVNGSTDGYLKVSFFGPFYGSYVVFELDREN 120

Query: 121 YQYAFISGPDTDYLWLLARTPTVSPEVIQQFVDMAKAKGFDTDSLIYVEQK 171
Y YAF+SGP+T+YLWLL+RTPTV ++ +F++M+K +GFDT+ LIYV+Q+
Sbjct: 121 YSYAFVSGPNTEYLWLLSRTPTVERGILDKFIEMSKERGFDTNRLIYVQQQ 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1705RTXTOXIND561e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 55.6 bits (134), Expect = 1e-10
Identities = 30/144 (20%), Positives = 49/144 (34%), Gaps = 9/144 (6%)

Query: 49 TVERDRLTLTAPVGELIHKINVVEGQQVKAGEVLLELDSTAVNARLAQRQAELKQA---- 104
T + ++ +I V EG+ V+ G+VLL+L + A + Q+ L QA
Sbjct: 91 THSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ 150

Query: 105 ---QAKFDEAVTGARFEDIDKARAVLNGANASVKEAKQSFERTQR--LFKTKVLSQADLD 159
Q E + S + Q K + +LD
Sbjct: 151 TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLD 210

Query: 160 AARAAKDTSLAKQAEAEQSLRLLQ 183
RA + T LA+ E R+ +
Sbjct: 211 KKRAERLTVLARINRYENLSRVEK 234



Score = 45.6 bits (108), Expect = 2e-07
Identities = 36/290 (12%), Positives = 87/290 (30%), Gaps = 46/290 (15%)

Query: 71 VEGQQVKAGEVLLELDSTAVNARLAQRQAELKQAQAKFDEAVTGARFEDIDKARAVLNGA 130
V ++V L++ + + Q++ L + +A + A +N
Sbjct: 179 VSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRA------------ERLTVLARINRY 226

Query: 131 NASVKEAKQSFERTQRLFKTKVLS--------------QADLDAARAAKDTSLAKQAEAE 176
+ K + L + ++ +L ++ + ++ A+
Sbjct: 227 ENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAK 286

Query: 177 QSLRLLQNGTRSEQIEQARSAVDVAIAAVALEQKALKDLSLVAAKH----AVVDTLPWRV 232
+ +L+ ++E +++ R D K + + V
Sbjct: 287 EEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTE 346

Query: 233 GDRVAAGSQLIGLLAIERPF-VRLYLPATWLDRVKAGSHVDILVDG----RAAPIAGTVR 287
G V L+ ++ + V + + + G + I V+ R + G V+
Sbjct: 347 GGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVK 406

Query: 288 NI-----RSQPAYTPFYALNERDRARLMYLTDIDIAEEGQSLATGMPLEV 332
NI Q F + + L + L++GM +
Sbjct: 407 NINLDAIEDQRLGLVFNVIISIEENCLS------TGNKNIPLSSGMAVTA 450


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1707ABC2TRNSPORT375e-05 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 37.2 bits (86), Expect = 5e-05
Identities = 42/166 (25%), Positives = 77/166 (46%), Gaps = 23/166 (13%)

Query: 186 GVILTMTMVMFT----SAAIVREREQGNMEFLITTPVRPLELMLGKI----TPYVLVGFV 237
G++ T M T AA R Q E ++ T +R +++LG++ T L G
Sbjct: 72 GMVATSAMTAATFETIYAAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAG 131

Query: 238 QVTIILSAGH-----LLFDVPIRGGIDSIAFAAMLFICASLTLGLVISTIAKTQLQSMQM 292
+ + G+ LL+ +P+ IA + F +LG+V++ +A + +
Sbjct: 132 IGVVAAALGYTQWLSLLYALPV------IALTGLAFA----SLGMVVTALAPSYDYFIFY 181

Query: 293 TVFILLPSILLSGFMFPYEAMPVAAQWIAEALPATHFMRMSRAIVL 338
++ P + LSG +FP + +P+ Q A LP +H + + R I+L
Sbjct: 182 QTLVITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIML 227


19SO_1788SO_1797Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1788220-0.685344tRNA-(MS(2)IO(6)A)-hydroxylase MiaE
SO_1789125-0.816449UDP-23-diacylglucosamine hydrolase LpxH
SO_1790226-0.935602cytoplasmic peptidyl-prolyl cis-trans isomerase
SO_17912210.039224cysteinyl-tRNA synthetase CysS
SO_1792323-0.205271bifunctional methylenetetrahydrofolate
SO_1793423-0.868829***trigger factor peptidyl-prolyl cis-trans
SO_1794218-0.661341ATP-dependent Clp protease proteolytic subunit
SO_1795318-0.400290ATP-dependent Clp protease ATP-binding subunit
SO_1796318-0.450414ATP-dependent protease La Lon
SO_1797217-0.649563histone-like DNA-binding protein HupB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1795HTHFIS310.009 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.009
Identities = 14/70 (20%), Positives = 28/70 (40%), Gaps = 13/70 (18%)

Query: 64 KLPTPHELRAHLDDYVIGQDRAKKVLSVAVYNHYKRLKNSSPKDGVELGKSNILLIGPTG 123
+ P+ E + ++G+ A +Y RL + +++ G +G
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAA----MQEIYRVLARLMQT---------DLTLMITGESG 170

Query: 124 SGKTLLAETL 133
+GK L+A L
Sbjct: 171 TGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1796HTHFIS330.006 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 32.9 bits (75), Expect = 0.006
Identities = 40/211 (18%), Positives = 77/211 (36%), Gaps = 37/211 (17%)

Query: 262 NMPSDAKEKAVAELNKLRMMSP---MSAEATV---VRSY----VDWMTSVPWSQRSKIKR 311
MP + + + K R P MSA+ T +++ D++ P+ I
Sbjct: 56 VMPDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPK-PFDLTELIGI 114

Query: 312 DLAKAQEVLDTDHYGLEKVKDRILEYLAVQSRVRQLKGPI---------LCLVGPPGVGK 362
+A LE + + + ++++ + L + G G GK
Sbjct: 115 I-GRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGK 173

Query: 363 TSLGQSIAKATGRK---YVRVALGGVRD---EAEIRGHRRTYIGSMPGKVIQKMAKVGVK 416
+ +++ R+ +V + + + E+E+ GH + G+ G + +
Sbjct: 174 ELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEK---GAFTGAQTRSTGRFEQA 230

Query: 417 N--PLFLLDEIDKMSSDMRGDPASALLEVLD 445
LFL DEI M D + + LL VL
Sbjct: 231 EGGTLFL-DEIGDMPMDAQ----TRLLRVLQ 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1797DNABINDINGHU1194e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 119 bits (300), Expect = 4e-39
Identities = 53/88 (60%), Positives = 69/88 (78%)

Query: 2 NKSELIEKIASGADISKAAAGRALDSFIAAVTEGLKEGDKISLVGFGTFEVRERAERTGR 61
NK +LI K+A +++K + A+D+ +AV+ L +G+K+ L+GFG FEVRERA R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGEEIKIAAAKIPAFKAGKALKDAV 89
NPQTGEEIKI A+K+PAFKAGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


20SO_1812SO_1825Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1812313-1.094235methionine gamma-lyase MdeA
SO_1813315-1.317676DNA competence protein ComEA
SO_1814113-0.290867late competence development protein ComFB
SO_1815011-0.479341histone deacetylase superfamily protein
SO_1816-210-0.916619periplasmic protein of unknown function DUF2057
SO_1817-314-2.107796primosomal replication protein N'-like protein
SO_1818-217-2.1163834-toluene sulfonate uptake permease family
SO_1819-120-2.738076ATP-dependent helicase DinG
SO_1820025-3.377886DNA polymerase II PolB
SO_1821231-4.000324outer membrane porin
SO_1822334-3.472313TonB-dependent receptor
SO_1824227-3.264901TonB2 energy transduction system periplasmic
SO_1825123-3.877568TonB2 energy transduction system inner membrane
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1821ECOLIPORIN771e-17 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 77.3 bits (190), Expect = 1e-17
Identities = 104/414 (25%), Positives = 164/414 (39%), Gaps = 51/414 (12%)

Query: 1 MNKTIVATALAALFLAPTVSAIEIYKDDKNAVEIGGFIDARVINTQGETEVVNG-ASRIN 59
M + ++A + AL A A EIY D N +++ G +D + ++ +G + +
Sbjct: 1 MKRKVLALVIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDDSSK--DGDQTYMR 58

Query: 60 FGFSRE--LSDGWNAFAKLEWGVNPVGNSDIVYSNRFESVQDEFFYNRLGYAGLSHDKYG 117
GF E ++D + + E+ V +N E + RL +AGL YG
Sbjct: 59 VGFKGETQINDQLTGYGQWEYNVQ---------ANTTEGEGANSW-TRLAFAGLKFGDYG 108

Query: 118 TLTIGKQWGAWYDVVYGTNYGFVWDGNTAGVYTFNKDDGAVNGVGRGDKTVQYRNT--FG 175
+ G+ +G YDV T+ + G++ Y N G NGV YRNT FG
Sbjct: 109 SFDYGRNYGVLYDVEGWTDMLPEFGGDSYT-YADNYMTGRANGV------ATYRNTDFFG 161

Query: 176 DV---SIAAQVQLKNSSFYTCDSVPDQDDCKK----LWETGDRSAQQVDYDETYGFAVTY 228
V + A Q Q KN S D ++ ++ GD YD GF+
Sbjct: 162 LVDGLNFALQYQGKNESQSADDVNIGTNNRNNGDDIRYDNGDGFGISTTYDIGMGFSAGA 221

Query: 229 KATDKLTLTAGVNRGEFDVSFGNGESTTAVDLIYGAGIMWGGFDANGWYAAA------NI 282
T VN G + G+ A + AG+ +DAN Y A N+
Sbjct: 222 AYTTSDRTNEQVNAG---GTIAGGDKADA----WTAGLK---YDANNIYLATMYSETRNM 271

Query: 283 NKQENHDTDNLGRLIKEAVGAETLLSYKFDNGLRYFVSYNILDAGKDYVIQPNLPIYAND 342
D G + + E Y+FD GLR VS+ ++ GKD + N+ D
Sbjct: 272 TPYGKTDKGYDGGVANKTQNFEVTAQYQFDFGLRPAVSF-LMSKGKD-LTYNNVNGDDKD 329

Query: 343 VFKRQFVVAGVHYLMDANTVIYLEGRKDFSDFTGVDEAAMALSEDDGIAIGIRY 396
+ K + G Y + N Y++ + + D +S DD +A+G+ Y
Sbjct: 330 LVK--YADVGATYYFNKNFSTYVDYKINLLDDDDPFYKDAGISTDDIVALGMVY 381


21SO_1924SO_1931Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_19242170.210142RND superfamily efflux pump permease component
SO_1925426-0.432118RND superfamily efflux pump MFP component
SO_19265310.589274citrate synthase GltA
SO_19276350.715302succinate dehydrogenase cytochrome b556 subunit
SO_47786370.754763succinate dehydrogenase membrane anchor subunit
SO_19286391.004857succinate dehydrogenase flavoprotein subunit
SO_19295350.883606succinate dehydrogenase iron-sulfur protein
SO_19304331.0064762-oxoglutarate dehydrogenase complex
SO_19314320.0486902-oxoglutarate dehydrogenase complex
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1924ACRIFLAVINRP6620.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 662 bits (1709), Expect = 0.0
Identities = 257/1090 (23%), Positives = 471/1090 (43%), Gaps = 92/1090 (8%)

Query: 7 SVKRPVTVWMFMLAIMLFGMVGFSRLAVKLLPDLSYPTLTIRTMYDGAAPVEVEQLVSKP 66
++RP+ W+ + +M+ G + +L V P ++ P +++ Y GA V+ V++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 67 IEEAVGVVKGLRKISSISRS-GMSDVVLEFEWGTTMDMASLDVREKLDTI--ALPLDVKK 123
IE+ + + L +SS S S G + L F+ GT D+A + V+ KL LP +V++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 124 PLLLRFNPNLDPIMRLALSVPNASEAELKQMRTYAEEELKRRLEALSGVAAVRLSGGLEQ 183
+ + +M N + Y +K L L+GV V+L G +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSDNPGT-TQDDISDYVASNVKDTLSRLNGVGDVQLFGA-QY 182

Query: 184 EVHIQLNQEKLSQLNLNADDIKRRINEENINLSAGKVIQGD------REYLVRTLNQFNS 237
+ I L+ + L++ L D+ ++ +N ++AG++ + +F +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKN 242

Query: 238 LDELGQVIVYRDAQ-TLVRLFEVATITDAYKERSDITRIGSQESIELAIYKEGDANTVAV 296
+E G+V + ++ ++VRL +VA + + + I RI + + L I AN +
Sbjct: 243 PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDT 302

Query: 297 AKKLRDELVKINKD-PQQNKLEVIYDQSEFIESAVSEVTSSALMGSVLAMLVIYLFLRNI 355
AK ++ +L ++ PQ K+ YD + F++ ++ EV + +L LV+YLFL+N+
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 356 IPTLIISISIPFSVIATFNMMYFADISLNIMSLGGIALAIGLLVDNAIVVLENIDRC-RS 414
TLI +I++P ++ TF ++ S+N +++ G+ LAIGLLVD+AIVV+EN++R
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 415 EGMSKLDAAVTGTKEVAGAIFASTMTTLAVFVPLVFVDGIAGALFSDQALTVTFALLASL 474
+ + +A ++ GA+ M AVF+P+ F G GA++ ++T+ A+ S+
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 475 LVALTSIPMLASREGFKILPELMKKTPKEKPTTKLGKLKHYSATVFSFPIVLLFNYLPSA 534
LVAL P L + L+K E K
Sbjct: 483 LVALILTPALCAT--------LLKPVSAEHHENK-------------------------- 508

Query: 535 LLTFVLIIGRFFSWLLGLIMRPLSSGFNFVYHSTESIYHKLLAIALRKQLATLLLTTGIT 594
G FF W FN + + + Y + L LL+ I
Sbjct: 509 --------GGFFGW------------FNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIV 548

Query: 595 GACISLLPRLGMELIPPMNQGEFYVEILLPPGTAVGETDRILQQLALSI--KDRPEVKHA 652
+ L RL +P +QG F I LP G T ++L Q+ ++ V+
Sbjct: 549 AGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESV 608

Query: 653 YSQAGSGGLMTSDTARGGENWGRLQVELVDHSAYHQVTQVLRDTARRIPALEAKIEQPEL 712
++ G + +N G V L + R KI +
Sbjct: 609 FTVNGFS------FSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFV 662

Query: 713 FSFKTPLEIEL---TGYDLALLKRSADSLVNALSASDRFA-----------DINTSLRDG 758
F P +EL TG+D L+ ++ A ++ + + +
Sbjct: 663 IPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLED 722

Query: 759 QPELSIRFDHARLAALGMDAPTVANRIAQRVGGTVASQYTVRDRKIDILVRSQLDERDQI 818
+ + D + ALG+ + I+ +GGT + + R R + V++ R
Sbjct: 723 TAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLP 782

Query: 819 SDIDTLIINPDSSQPIALSAVAEVSLQLGPSAINRISQQRVALVSANLAYGDLSDAVADA 878
D+D L + + + + SA G + R + + A G S
Sbjct: 783 EDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMAL 842

Query: 879 QQILAAQVLPTSVQARFGGQNEEMEHSFQSLKIALILAVFLVYLVMASQFESLLHPLLIL 938
+ LA++ LP + + G + + S + ++ +V+L +A+ +ES P+ ++
Sbjct: 843 MENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVM 901

Query: 939 VAVPMALAGSVLGLYITQTHLSVVVFIGLIMLAGIVVNNAIVLVDRINQL-RSEGVEKLE 997
+ VP+ + G +L + V +GL+ G+ NAI++V+ L EG +E
Sbjct: 902 LVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVE 961

Query: 998 AIRVAAKSRLRPIMMTTLTTVLGLLPMALGLGDGAEVRAPMAITVIFGLSLSTLLTLIVI 1057
A +A + RLRPI+MT+L +LG+LP+A+ G G+ + + I V+ G+ +TLL + +
Sbjct: 962 ATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFV 1021

Query: 1058 PVLYAMFDRK 1067
PV + + R
Sbjct: 1022 PVFFVVIRRC 1031



Score = 114 bits (286), Expect = 1e-27
Identities = 95/520 (18%), Positives = 195/520 (37%), Gaps = 38/520 (7%)

Query: 578 IALRKQLATLLLTTGITGACISLLPRLGMELIPPMNQGEFYVEILLPPGTAVGETDRILQ 637
+R+ + +L + A + +L + P + V P A D + Q
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 638 QLALSIKDRPEVKHAYSQAGSGGLMTSDTARGGENWGRLQVELVDHSAYHQVTQVLRDTA 697
+ ++ + + S + S G +T L + QV
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTIT----------LTFQS-GTDPDIAQVQVQNKLQ 112

Query: 698 RRIPALEAKIEQPELFSFKTP----LEIELTGYDLALLKRS-----ADSLVNALSASDRF 748
P L +++Q + K+ + + + A ++ + LS +
Sbjct: 113 LATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGV 172

Query: 749 ADINTSLRDGQPELSIRFDHARLAALGMDAPTVANRI----AQRVGGTVASQYTVRDRKI 804
D+ Q + I D L + V N++ Q G + + +++
Sbjct: 173 GDVQLF--GAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQL 230

Query: 805 D--ILVRSQLDERDQISDIDTLIINPDSSQPIALSAVAEVSLQLGPSAIN-RISQQRVAL 861
+ I+ +++ ++ + TL +N D S + L VA V L + RI+ + A
Sbjct: 231 NASIIAQTRFKNPEEFGKV-TLRVNSDGS-VVRLKDVARVELGGENYNVIARINGKPAAG 288

Query: 862 VSANLAYGDLSDAVADA-QQILA--AQVLPTSVQA-RFGGQNEEMEHSFQSLKIALILAV 917
+ LA G + A A + LA P ++ ++ S + L A+
Sbjct: 289 LGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAI 348

Query: 918 FLVYLVMASQFESLLHPLLILVAVPMALAGSVLGLYITQTHLSVVVFIGLIMLAGIVVNN 977
LV+LVM +++ L+ +AVP+ L G+ L ++ + G+++ G++V++
Sbjct: 349 MLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDD 408

Query: 978 AIVLVDRINQ-LRSEGVEKLEAIRVAAKSRLRPIMMTTLTTVLGLLPMALGLGDGAEVRA 1036
AIV+V+ + + + + + EA + ++ + +PMA G +
Sbjct: 409 AIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYR 468

Query: 1037 PMAITVIFGLSLSTLLTLIVIPVLYAMF--DRKKFDHTNI 1074
+IT++ ++LS L+ LI+ P L A H N
Sbjct: 469 QFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1925RTXTOXIND476e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.1 bits (112), Expect = 6e-08
Identities = 30/195 (15%), Positives = 65/195 (33%), Gaps = 15/195 (7%)

Query: 89 QSLAIIDAKRQQYDLDRSEAEVKIIEQELNRLKKMNNKEFISADSMAKLEYNLQAAIARR 148
+ + Y + E +I+ + + D + + N+
Sbjct: 259 NKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318

Query: 149 DLAELQVKESHVVSPIDGIIAKRYVKAGNMAKEFGD-LFYIV-NQDELHGIVHLPEQQLT 206
E + + S + +P+ + + V + L IV D L + + +
Sbjct: 319 AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIG 378

Query: 207 SLRLGQEAQV-FS--NQQSKNAINAKVLRISP--VVDPQSGT-FKVTLAVP-------NQ 253
+ +GQ A + + KV I+ + D + G F V +++ N+
Sbjct: 379 FINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNK 438

Query: 254 DARLKAGMFTRVELK 268
+ L +GM E+K
Sbjct: 439 NIPLSSGMAVTAEIK 453



Score = 40.6 bits (95), Expect = 6e-06
Identities = 19/84 (22%), Positives = 34/84 (40%), Gaps = 9/84 (10%)

Query: 37 PVETTTVIQGNVSSFYSTTATLEAPQEANVVSRIAGLIEVINVEEGDRVKKGQSLAIIDA 96
VE G ++ S + P E ++V I V+EG+ V+KG L + A
Sbjct: 79 QVEIVATANGKLTH--SGRSKEIKPIENSIVKEII-------VKEGESVRKGDVLLKLTA 129

Query: 97 KRQQYDLDRSEAEVKIIEQELNRL 120
+ D ++++ + E R
Sbjct: 130 LGAEADTLKTQSSLLQARLEQTRY 153


22SO_1942SO_1955Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1942-117-3.241351cyclic di-GMP hydrolase
SO_1944326-4.4940562OG-Fe(II) oxygenase family protein
SO_1945329-4.816338two component signal transduction system
SO_1946327-3.781409two component signal transduction system
SO_1947224-3.254680hypothetical protein
SO_1948223-3.172120glutamate/aspartate:proton symporter GltP
SO_1949224-3.327894invasin domain protein
SO_1950115-1.253738ISSod11 transposase TnpA_ISSod11
SO_1952114-0.018799gamma-glutamyltransferase GgtA
SO_1953120-1.161341protein of unknown function DUF482
SO_1954220-1.531514homogentisate export protein
SO_1955222-1.983588hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1945PF06580347e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 34.5 bits (79), Expect = 7e-04
Identities = 19/102 (18%), Positives = 34/102 (33%), Gaps = 23/102 (22%)

Query: 356 LMENAFRLCISQ------VQVTAHFNEQGDFELIVEDDGPGVEEQLRQKIIQRGVRADTQ 409
L+EN + I+Q + + + G L VE+ G + ++
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKG-TKDNGTVTLEVENTGSLALKNTKE------------ 309

Query: 410 SPGQGIGLA-VCDEIVSSYGGSLKIE-ESHLEGALFRITIPA 449
G GL V + + YG +I+ + IP
Sbjct: 310 --STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIPG 349


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1946HTHFIS772e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.8 bits (189), Expect = 2e-18
Identities = 31/120 (25%), Positives = 57/120 (47%), Gaps = 1/120 (0%)

Query: 2 RILVVEDDLILSHHLKVQLSDLGNQVQVALTAKEGFFQATNYPIDVAIVDLGLPDQDGIS 61
ILV +DD + L LS G V++ A + D+ + D+ +PD++
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 LIQQLREEGVKAPILILTARVNWQDKVEGLNAGADDYLVKPFQKEELVARLD-ALVRRSA 120
L+ ++++ P+L+++A+ + ++ GA DYL KPF EL+ + AL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1949INTIMIN451e-06 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 45.4 bits (107), Expect = 1e-06
Identities = 57/290 (19%), Positives = 99/290 (34%), Gaps = 28/290 (9%)

Query: 384 TTNISANQPAKVTVTL-VDKDSIPLSGKVVSFASSLGNFLPSKGTALTDSIGRASITLTA 442
T+ A+ +T T V K+ + + VSF G + S +A T+ G+A++TL +
Sbjct: 567 KTSAKADGTEAITYTATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVTLKS 626

Query: 443 GSIEGAGEITATYGTAKAIIGFATAGDEIDPVEASPEITFDIYNCNGVAAWDKTLKNFEV 502
G++ + TA + + A+ I D E+
Sbjct: 627 DKP---GQVVVSAKTA----------EMTSALNANAVIFVD----------QTKASITEI 663

Query: 503 CQPTDNITNDKPGIIGAKVTRSGSTQPLQQVLVTAATTLGAISPNSGTAITNADGKAILD 562
+ I V +P+ VT TTLG ++ T T+ +G A +
Sbjct: 664 KADKTTAVANGQDAITYTVKVMKGDKPVSNQEVTFTTTLGK--LSNSTEKTDTNGYAKVT 721

Query: 563 LYANGSVGAGEVSLKVKDVTSTKAFEIGRVNISLKLETSLGTNLLPAGGSTILDVTVLNP 622
L + + G VS +V DV +L ++ + + V +
Sbjct: 722 LTST-TPGKSLVSARVSDVAVDVKAPEVEFFTTLTIDDGNIEIVGTGVKGKLPTVWLQYG 780

Query: 623 DGSL-ATGQPFTLVFTSECQASNKAIIDSPVITNGGKGYATYRSTGCETQ 671
+L A+G + S A S +T KG T + Q
Sbjct: 781 QVNLKASGGNGKYTWRSANPAIASVDASSGQVTLKEKGTTTISVISSDNQ 830



Score = 44.3 bits (104), Expect = 3e-06
Identities = 67/344 (19%), Positives = 126/344 (36%), Gaps = 39/344 (11%)

Query: 55 ATPAEVKATVVDSKTGPKAGVVVTFKLDNASLGIFTPATGTQLTDSSGVATIKLETATLA 114
ATV + +A V V+F + + G + + T+ SG AT+ L++
Sbjct: 575 TEAITYTATVKKNGV-AQANVPVSFNIVS---GTAVLSANSANTNGSGKATVTLKSDKP- 629

Query: 115 GAGNVTASIATGESATKGFFSKGDGVTNPGTGNKLALSLVNQQQQVITGISAAVRGIIKA 174
G V+A A SA + T + + + + A+ +K
Sbjct: 630 GQVVVSAKTAEMTSALNANAV----IFVDQTKASIT-EIKADKTTAVANGQDAITYTVKV 684

Query: 175 SYTNSLNEPLVGKVVVFNSTLGKLSPESGTALTNSQGIAEISITAGTIAGAGKITAKVDG 234
++P+ + V F +TLGKLS + T T++ G A++++T+ T G ++A+V
Sbjct: 685 MKG---DKPVSNQEVTFTTTLGKLS--NSTEKTDTNGYAKVTLTSTT-PGKSLVSARVSD 738

Query: 235 TEAEPLGFNTLGDEVVVKPIDSYAITLNIQDSQGNNLRKISHSVPGSVVATLLKDG---- 290
+ V P + TL I N+ + V G + L+ G
Sbjct: 739 VAVD-----------VKAPEVEFFTTLTI---DDGNIEIVGTGVKGKLPTVWLQYGQVNL 784

Query: 291 -VPASYQKISFNL--NGEGILNPSSGTALTDLSGRALVTLVTGTNAGAATVTASFSLDND 347
K ++ ++ SSG G +++++ N AT T + + ++
Sbjct: 785 KASGGNGKYTWRSANPAIASVDASSGQVTLKEKGTTTISVISSDNQ-TATYTIA-TPNSL 842

Query: 348 IITDSFNAEVAGDAPGGNGEANSLSIQLTNSLTGLSTTNISANQ 391
I+ + DA N L + +AN+
Sbjct: 843 IVPNMSKRVTYNDAVNTCKNFGGKLPSSQNELENVFKAWGAANK 886


23SO_2065SO_2072Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_20650233.574623tyrosine transporter TyrP
SO_20671244.146609bifunctional phosphoribosyl-AMP
SO_20682234.575689imidazole glycerol phosphate synthase cyclase
SO_20692223.9275261-(5-phosphoribosyl)-5-[(5-
SO_20702212.674966imidazole glycerol phosphate synthase glutamine
SO_20712191.911713bifunctional imidazoleglycerol-phosphate
SO_20722160.413496histidinol-phosphate aminotransferase HisC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2070cdtoxinb280.037 Cytolethal distending toxin B signature.
		>cdtoxinb#Cytolethal distending toxin B signature.

Length = 269

Score = 27.7 bits (61), Expect = 0.037
Identities = 12/31 (38%), Positives = 18/31 (58%)

Query: 131 GLPLPHMGWNQLTFSNPSQVHPLFAGVEAGS 161
G+P+ + WN T S P QV+ F+ V+A
Sbjct: 81 GIPVRELIWNLSTNSRPQQVYIYFSAVDALG 111


24SO_2114SO_2124Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2114214-2.615162outer membrane protein Omp85 family
SO_2115116-3.241219bifunctional isoaspartyl dipeptidase /
SO_2116317-3.953654acetyl-CoA synthetase acetylase YfiQ
SO_2117325-6.628302chemotaxis signal transduction system methyl
SO_2118223-5.472183chemotaxis locus anti-sigma factor antagonist
SO_2119222-5.097546protein phosphatase with response regulator
SO_2120119-3.424137chemotaxis signal transduction system response
SO_2121118-2.925922chemotaxis signal transduction system histidine
SO_2122018-3.154094chemotaxis signal transduction system adaptor
SO_2123-118-3.239740chemotaxis signal transduction system methyl
SO_2124019-3.369902chemotaxis signal transduction system MCP
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2119HTHFIS639e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.3 bits (154), Expect = 9e-13
Identities = 26/122 (21%), Positives = 52/122 (42%)

Query: 11 ILIVDNDAIASQSISDFIHGKGYNVIICDNLEDAFFEVSLNKIDLILVNYFQPDGTALTL 70
IL+ D+DA ++ + GY+V I N + ++ DL++ + PD A L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 71 LAHLDSLSKEIPVVVINDKKEPQAFLDCFKMGVLDFIVKPINVEVFWYKAEILLTRIKLQ 130
L + ++PV+V++ + + + G D++ KP ++ L K +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 131 RK 132

Sbjct: 126 PS 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2120HTHFIS872e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 2e-23
Identities = 30/122 (24%), Positives = 56/122 (45%), Gaps = 3/122 (2%)

Query: 1 MSK-KILIVDDSAAIRQMVEATLKSANYQVTLAKDGREALDLCSSQRFDFILTDQNMPRM 59
M+ IL+ DD AAIR ++ L A Y V + + ++ D ++TD MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 DGLTLIKSLRGMSAFMRTPIIMLTTEAGDDMKAQGKAVGATGWMVKPFDPQKLLAITAKV 119
+ L+ ++ A P+++++ + + GA ++ KPFD +L+ I +
Sbjct: 61 NAFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 120 LG 121
L
Sbjct: 119 LA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2121PF06580358e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 35.2 bits (81), Expect = 8e-04
Identities = 13/66 (19%), Positives = 31/66 (46%), Gaps = 10/66 (15%)

Query: 418 EIDKGMIEKLVDPLT--HLVRNSLDHGIEKPEVRRLLGKAEIAQLSLRASQRGGNIVIAV 475
+I+ +++ V P+ LV N + HGI + + ++ L+ ++ G + + V
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQ--------LPQGGKILLKGTKDNGTVTLEV 296

Query: 476 HDDGAG 481
+ G+
Sbjct: 297 ENTGSL 302


25SO_2311SO_2324Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2311015-3.075211hypothetical protein
SO_2312116-3.895269serine protease inhibitor ecotin EcoT
SO_2314117-4.122026ISSod1 transposase TnpA_ISSod1
SO_2318018-5.251025chemotaxis signal transduction system response
SO_2319019-5.553233chemotaxis locus antisigma factor antogonist
SO_2321-120-4.451452ISSod4 transposase TnpA_ISSod4
SO_2323-120-4.449154chemotaxis signal transduction system methyl
SO_2324016-3.127340chemotaxis signal transduction system adaptor
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2318HTHFIS995e-28 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 99 bits (249), Expect = 5e-28
Identities = 30/115 (26%), Positives = 56/115 (48%), Gaps = 2/115 (1%)

Query: 3 KILVVDDSASIRHVVSIALRGAGYEVIDACDGKDALSKLNGDKINLIISDVNMPNMDGIS 62
ILV DD A+IR V++ AL AGY+V + + +L+++DV MP+ +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 FLKEVKKHPRYKFTPVIMLTTEAGRDKMEEGRMAGAKAWVVKPFQPPQMLDAVAK 117
L +KK PV++++ + + GA ++ KPF +++ + +
Sbjct: 65 LLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2323SECFTRNLCASE359e-04 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 34.8 bits (80), Expect = 9e-04
Identities = 22/109 (20%), Positives = 42/109 (38%), Gaps = 1/109 (0%)

Query: 343 GDLTIELKQREQAT-GVYKAMIDMIDSLTSVIAQVRSGADNLSSASSQVSSTAQSLSQGA 401
G TI + GVY+A ++ ++ +I++VR + + + Q QGA
Sbjct: 51 GGTTIRTESTTAIDVGVYRAALEPLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGA 110

Query: 402 TEQAASVEETTSAVEELNASVQQNSENARVTNNMATVAAEEARQGGIAV 450
Q A +E + VE +V + + V+ E ++
Sbjct: 111 EGQGAQGQELVNKVETALTAVDPALKITSFESVGPKVSGELVWTAVWSL 159


26SO_2360SO_2380Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2360427-2.598122Cbb3-type cytochrome oxidase assembly protein
SO_2361427-3.232823Cbb3-type cytochrome c oxidase subunit III CcoP
SO_2362428-3.822382Cbb3-type cytochrome c oxidase subunit IV CcoQ
SO_2363423-3.394239Cbb3-type cytochrome c oxidase subunit II CcoO
SO_2364218-3.819865Cbb3-type cytochrome c oxidase subunit I CcoN
SO_2365019-4.058829predicted lipoprotein
SO_2366-117-3.795226two component signal transduction system
SO_4819-116-2.800567hypothetical protein
SO_2368-115-2.906160ISSod1 transposase TnpA_ISSod1
SO_2373-115-3.188106*drug:H+ antiporter DHA1 family
SO_2374-118-2.141232transcriptional regulator YdhB
SO_2375-215-3.200880integral membrane FtsH interacting protein YccA
SO_2376-217-3.696831tRNA 2-thiouridine synthesizing protein D
SO_2377-218-4.678829tRNA 2-thiouridine synthesizing protein C
SO_2378-120-5.002184tRNA 2-thiouridine synthesizing protein B
SO_2379-119-3.777159tRNA 2-thiouridine synthesizing protein sulfur
SO_2380-219-3.423245ATP-dependent DNA helicase RecQ family
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2366HTHFIS486e-08 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 47.9 bits (114), Expect = 6e-08
Identities = 23/90 (25%), Positives = 38/90 (42%), Gaps = 9/90 (10%)

Query: 28 KILLVDDEPDVHTVTKLALSRFKLDGRALSFINAYSAEQAKEFLINEQDLAIAFIDVVME 87
IL+ DD+ + TV ALSR D R +A I D + DVVM
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRI-----TSNAATLWR-WIAAGDGDLVVTDVVMP 58

Query: 88 TDHAGLELVKWIREDHKNKTIRLILRTGQP 117
D +L+ I++ + + +++ + Q
Sbjct: 59 -DENAFDLLPRIKKARPD--LPVLVMSAQN 85


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2373TCRTETB673e-14 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 67.2 bits (164), Expect = 3e-14
Identities = 77/388 (19%), Positives = 140/388 (36%), Gaps = 51/388 (13%)

Query: 10 NMKFFIFLLYLALLSMLGFIATDMYLPAFKAIESSLNSSPSQVAMSLTCFLAGLALGQLI 69
N++ L++L +LS + + + I + N P+ T F+ ++G +
Sbjct: 9 NLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAV 68

Query: 70 YGPLVSKLGKRYALLIGLAVFALSSVAIANSDSVMMLNI-ARFFQAIGACSAGVIWQAIV 128
YG L +LG + LL G+ + SV S L I ARF Q GA + + +V
Sbjct: 69 YGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVV 128

Query: 129 VEQYDAEKAQGIFSNIMPLVALSPALAPILGAYILNEFGWRAIFISLCVIAFLLVLMTLY 188
E F I +VA+ + P +G I + W + + + +I + V +
Sbjct: 129 ARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLL-IPMITIITVPFLMK 187

Query: 189 FVPGKSKHQD----------------------------IKPSAVSYG------------- 207
+ + + + + S +S+
Sbjct: 188 LLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPF 247

Query: 208 ---QILKNTRYLGNVVIFGACSGAFFAYLTVWPIVMEQ-HGYQATEIGLSFI-PQTIMFI 262
+ KN ++ V+ G G ++++ P +M+ H EIG I P T+ I
Sbjct: 248 VDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVI 307

Query: 263 VGGYASKLLIKRIGADKTLNVLLSIFGICVVSIVLFTLLFKTITIFPLLISFSILAAANG 322
+ GY +L+ R G LN+ ++ + ++ ++ L+
Sbjct: 308 IFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKT 367

Query: 323 AIYPIVVNSALQQFTQHASKAAGLQNFL 350
I IV +S Q Q A L NF
Sbjct: 368 VISTIVSSSLKQ---QEAGAGMSLLNFT 392


27SO_2537SO_2550Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2537-115-3.311042sodium:proton antiporter CPA1 family
SO_2538020-5.744344two component signal transduction system
SO_2539027-8.552542two component signal transduction system
SO_2540131-9.627803two component signal transduction system
SO_2541130-9.670411two component signal transduction system
SO_2542232-10.429789signaling protein with FIST domain
SO_2543335-11.700556two component signal transduction system
SO_2544434-11.469040two component signal transduction system hybrid
SO_2545123-7.327424two component signal transduction system
SO_2546121-4.467126chemotaxis signal transduction system inhibitor
SO_2547022-4.912725chemotaxis signal transduction system response
SO_4827127-6.365743protein of unknown function DUF3309
SO_2549-121-3.802808osmotic shock-inducible lipoprotein OsmY
SO_2550-118-3.072866cAMP binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2537IGASERPTASE373e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 37.0 bits (85), Expect = 3e-04
Identities = 20/77 (25%), Positives = 33/77 (42%), Gaps = 9/77 (11%)

Query: 594 ALEEAKIQQMIAEQEAIAAQTKAAEEATLAKAKAEEKAEVERQRLDQQAQM----KAKQS 649
+ ++ Q E QT +E A + EEKA+VE ++ + ++ KQ
Sbjct: 1079 NTQTNEVAQS--GSETKETQTTETKET--ATVEKEEKAKVETEKTQEVPKVTSQVSPKQE 1134

Query: 650 QSEHE-PQDAIDRSDET 665
QSE PQ R ++
Sbjct: 1135 QSETVQPQAEPARENDP 1151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2538HTHFIS492e-08 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 49.1 bits (117), Expect = 2e-08
Identities = 32/155 (20%), Positives = 63/155 (40%), Gaps = 12/155 (7%)

Query: 4 VLFVDDDSFMLRALLRLAKRLRPEWQ-FWTEEDGLNWAKSIPHNVNIDLIVCDYLMPDIN 62
+L DDD+ + L + R + + W + DL+V D +MPD N
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD----GDLVVTDVVMPDEN 61

Query: 63 GDSVLIEASKHFPLAIRALLTGDTTEEVVCKAGKA-AHFVLSKPFNEQDIVQLL-TCIER 120
+L K P +++ T KA + A+ L KPF+ +++ ++ +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 121 IHKLPFTHEVRA-----MLGASALLLPLPDIVQRV 150
+ P E + ++G SA + + ++ R+
Sbjct: 122 PKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2539HTHFIS1024e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 102 bits (256), Expect = 4e-25
Identities = 39/134 (29%), Positives = 64/134 (47%), Gaps = 2/134 (1%)

Query: 1 MDK-SLLLVDDDVGILKALTRLLTRSGYSVKTAQSGEEALTLLLNYDCKVVLTDFRMPYM 59
M ++L+ DDD I L + L+R+GY V+ + + D +V+TD MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 DGGQLLSKIKRLYPDIVSLVISGYSDFESVKSLLNAGSAYRFLQKPWEDDELLGEIANAF 119
+ LL +IK+ PD+ LV+S + F + G AY +L KP++ EL+G I A
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKG-AYDYLPKPFDLTELIGIIGRAL 119

Query: 120 THYAKHLFQHQSQK 133
+ + +
Sbjct: 120 AEPKRRPSKLEDDS 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2540HTHFIS895e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.7 bits (220), Expect = 5e-23
Identities = 28/116 (24%), Positives = 58/116 (50%), Gaps = 3/116 (2%)

Query: 73 DKKSILIIDDELSMRNALRRALQSTPFTILTAQDGFQAGVKVIAEKPDLILLDLSLPGLD 132
+IL+ DD+ ++R L +AL + + + + A DL++ D+ +P +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 133 GFEVIQFIRQRPDLAKLKILVLSGLSSIELA-ESIRLGADDAIAKPFDNHDLLDRV 187
F+++ I++ L +LV+S ++ A ++ GA D + KPFD +L+ +
Sbjct: 62 AFDLLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGII 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2541HTHFIS886e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.0 bits (218), Expect = 6e-21
Identities = 31/159 (19%), Positives = 67/159 (42%), Gaps = 5/159 (3%)

Query: 12 ILCVDDEASILKSLQRLFIGKDLQILLADSGSKALELMLEHRVNVIITDMRMPNMTGAEF 71
IL DD+A+I L + + + + + + ++++TD+ MP+ +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 72 LAKAAILQPDAYRILMTGYADLASTVSAINLGKIHRYVQKPWDNQELLTVVDEGLALCHL 131
L + +PD ++M+ + + A G + Y+ KP+D EL+ ++ AL
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKG-AYDYLPKPFDLTELIGIIGR--ALAEP 122

Query: 132 IRQNKQLTAKVATQNKQLKELNSSLEETVLKRTEQLKQT 170
R+ +L + S+ + + + +L QT
Sbjct: 123 KRRPSKLEDDSQDGMPLVGR--SAAMQEIYRVLARLMQT 159


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2543PF06580364e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 35.6 bits (82), Expect = 4e-04
Identities = 31/180 (17%), Positives = 69/180 (38%), Gaps = 28/180 (15%)

Query: 283 LAGARRARDIIKNL-----RNFSHPDENTISTINILELITDTVRIANTQVKKHARIKINH 337
L +AR+++ +L + + + +S + L ++ +++A ++ R++ +
Sbjct: 187 LEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLA--SIQFEDRLQFEN 244

Query: 338 DLHHAFTQGNATQLSQVILNLINNA-HHSIKH--QHGLIEISINKFNNWINIEIEDNGCG 394
++ A + ++ L+ N H I Q G I + K N + +E+E+ G
Sbjct: 245 QINPAI--MDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSL 302

Query: 395 IDDTDIPHIFEPFFTTKEIGQGTGLGLSISRAIIEQHNGCIALVHTGLK--GTKFVISLP 452
K + TG GL R ++ G A + K ++ +P
Sbjct: 303 A--------------LKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2544HTHFIS672e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.2 bits (164), Expect = 2e-13
Identities = 26/112 (23%), Positives = 45/112 (40%), Gaps = 2/112 (1%)

Query: 807 HVLIVDDVEDIRELIDIYLKDTEIAVDFAQNGQQAIQLVEKSHYDLVILDQQMPIMDGFT 866
+L+ DD IR +++ L V N + + DLV+ D MP + F
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 867 AAKAIREFNKSIPLLLLSA--DILDTEPHQKSPFNKTIAKPFTKNQLIETIR 916
I++ +P+L++SA + + + KPF +LI I
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2545PF06580290.038 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.1 bits (65), Expect = 0.038
Identities = 19/96 (19%), Positives = 34/96 (35%), Gaps = 17/96 (17%)

Query: 327 NLLVNAAQAIEERGEISIDVSASDAEFIIVIRDTGSGIAASDLRKIFEPFYTTKLVGTGT 386
N + + + + G+I + + + + + +TGS K T
Sbjct: 266 NGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLAL--------------KNTKEST 311

Query: 387 GLGLSLSYSIVQKHKGE---IKVSSVLGEGTAFTVI 419
G GL +Q G IK+S G+ A +I
Sbjct: 312 GTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2547HTHFIS613e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 61.4 bits (149), Expect = 3e-14
Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 5/112 (4%)

Query: 5 VTIADDSLMSRKAVRRALPEDWDVEITEACNGKEALEAANSGKAEVLFLDLTMPELDGFG 64
+ +ADD R + +AL ++ N +G +++ D+ MP+ + F
Sbjct: 6 ILVADDDAAIRTVLNQAL-SRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 65 VLKYLHEQQSKTVVIVISADIQPEAKLLVDSL--GAFRFLQKPLQPAQLREA 114
+L + + + V+V+SA Q + + GA+ +L KP +L
Sbjct: 65 LLPRIKKARPDLPVLVMSA--QNTFMTAIKASEKGAYDYLPKPFDLTELIGI 114


28SO_2563SO_2569Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2563019-3.177041hydroxyacylglutathione hydrolase GloB
SO_2564-116-3.966846membrane-bound lytic peptidoglycan
SO_2565015-3.310139intracellular proteinase inhibitor domain
SO_2566015-3.722865outer membrane protein assembly protein AsmA
SO_2567-114-3.296348Rra-like regulator of RNAse E
SO_2569-114-3.245992putative cytoplasmic protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2565TYPE3IMSPROT270.048 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 27.4 bits (61), Expect = 0.048
Identities = 9/36 (25%), Positives = 14/36 (38%), Gaps = 1/36 (2%)

Query: 109 NAKLVIHNPAPHAISLRYHSG-MTADLVLTTEQGQR 143
+ +V+ NP AI + Y G LV +
Sbjct: 256 RSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQ 291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2569TACYTOLYSIN260.036 Bacterial thiol-activated pore-forming cytolysin sig...
		>TACYTOLYSIN#Bacterial thiol-activated pore-forming cytolysin

signature.
Length = 574

Score = 26.1 bits (57), Expect = 0.036
Identities = 17/76 (22%), Positives = 37/76 (48%), Gaps = 3/76 (3%)

Query: 14 LDEYGYEKKASTDLEQA--RNKQQMGKYIKSLDYSLRRLLILQ-ETVNELVEEKKHQLSQ 70
L+ E+K S D +++ + +++ I SL+Y+ +L ET+ V ++ + +
Sbjct: 88 LESAEKEEKKSEDNKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKAD 147

Query: 71 QENIQTYKTKIINLSR 86
+ + K K IN +
Sbjct: 148 KFIVIERKKKNINTTP 163


29SO_2644SO_2698Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2644217-0.904139phosphoenolpyruvate synthase PpsA
SO_2645013-1.986214putative phosphotransferase YdiA
SO_2646-115-2.078329phospho-2-dehydro-3-deoxyheptonate aldolase
SO_2647-117-2.9078084-hydroxybenzoyl-CoA thioesterase family
SO_2648016-0.809489two component signal transduction system
SO_26491160.019727transcriptional activator of cys regulon CysB
SO_26501200.472154hypothetical protein
SO_26522251.355271Mu phage transcriptional regulator Cro/CI family
SO_26534272.129450Mu phage transcriptional regulator Ner
SO_26544272.296626Mu phage transposase OrfA TnpA_MuSo2
SO_26553301.691970Mu phage transposase OrfB TnpB_MuSo2
SO_26564320.652676Mu phage uncharacterized protein
SO_26573341.431113Mu phage uncharacterized protein
SO_26583331.421858Mu phage uncharacterized protein
SO_2659026-0.267322Mu phage protein Kil
SO_2660224-1.292920Mu phage protein of unknown function DUF3164
SO_4787426-1.402603Mu phage uncharacterized protein
SO_2661423-3.801549Mu phage uncharacterized protein
SO_4788420-3.174897Mu phage uncharacterized protein
SO_2663224-4.406348Mu phage uncharacterized protein
SO_2664322-3.703535Mu phage uncharacterized protein
SO_2665224-3.445577Mu phage uncharacterized protein
SO_2666227-6.059715Mu phage uncharacterized protein
SO_2667123-3.274867Mu phage host gene modulation protein GemA
SO_4789323-3.575407Mu phage uncharacterized protein
SO_2668320-1.264646Mu phage middle operon regulator Mor
SO_2669321-1.026584Mu phage protein of unknown function
SO_2670320-0.682354Mu phage periplasmic protein of unknown
SO_26712201.794406Mu phage endolysin Lys
SO_26722182.076944Mu phage uncharacterized protein E18
SO_26733180.956146Mu phage uncharacterized protein
SO_26742181.464176Mu phage Mom translational regulator Com
SO_26751192.549467Mu phage protein of unknown function DUF2730
SO_26761202.945012Mu phage uncharacterized protein Gp26
SO_26771202.466379Mu phage small terminase subunit GpD
SO_26781202.183498Mu phage uncharacterized protein
SO_26791202.836407Mu phage large terminase subunit GpE
SO_26801222.598299Mu phage portal protein GpH
SO_26812221.677364Mu phage uncharacterized protein GpF
SO_26824231.057689Mu phage uncharacterized protein
SO_26835232.077839Mu phage uncharacterized protein
SO_26845211.862317Mu phage protease GpI
SO_26854221.842535Mu phage major head subunit GpT
SO_26864182.430613Mu phage uncharacterized protein
SO_26874182.346765Mu phage uncharacterized protein
SO_26882203.649324Mu phage uncharacterized protein
SO_26892213.620539Mu phage protein of unknown function DUF1320
SO_26901213.005326Mu phage tail completion protein GpG
SO_26910190.274507Mu phage uncharacterized protein
SO_26921190.627049Mu phage uncharacterized protein Gp38
SO_26930181.078867Mu phage tail sheath protein GpL
SO_2694018-0.361212Mu phage tail tube protein GpM
SO_2695019-0.386041Mu phage uncharacterized protein Gp41
SO_26971180.658814Mu phage tape measure protein Gp42
SO_26983182.334772Mu phage DNA circulation protein GpN
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2644PHPHTRNFRASE3031e-95 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 303 bits (778), Expect = 1e-95
Identities = 111/418 (26%), Positives = 187/418 (44%), Gaps = 65/418 (15%)

Query: 384 QPGDVLVTDMTDPDWEPIMK-RASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTDRIK 442
+ ++ D+T D + K T+ GGRT H+AI++R L +PAVVG +VT++I+
Sbjct: 155 EETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQ 214

Query: 443 NGQWVTVSCAEG---------DTGFIYEGKQEFEVVSNRVDALPALP--------MKIMM 485
+G V V EG + E + FE L P +++
Sbjct: 215 HGDMVIVDGIEGIVIVNPTEEEVKAYEEKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAA 274

Query: 486 NVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNQQDAALQEEINEMIAGY 545
N+G P EG+GL R EF+ + +D EE E Y
Sbjct: 275 NIGTPKDVDGVLANGGEGIGLYRTEFL--------------YMDRDQLPTEE--EQFEAY 318

Query: 546 DSPVEFYIARLVEGIASIGSAFYPKKVIVRMSDFKSNEYANLVGGDRYEPEEENPMLGFR 605
V+ K V++R D ++ + + P+E NP LGFR
Sbjct: 319 KEVVQ---------------RMDGKPVVIRTLDIGGDKELSYL----QLPKELNPFLGFR 359

Query: 606 GASRYISESFRDCFALECEAIKRVRNEMGLKNVEVMIPFVRTVKEAEQVIELLKEQGLER 665
+ + +D F + A+ R N++VM P + T++E Q +++E+ +
Sbjct: 360 AIRLCLEK--QDIFRTQLRALLRAS---TYGNLKVMFPMIATLEELRQAKAIMQEEKDKL 414

Query: 666 GKDG------LRVIMMCEVPSNALLADQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHL 719
+G + V +M E+PS A+ A+ F + D FSIG+NDL Q T+ DR + +S+L
Sbjct: 415 LSEGVDVSDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYL 474

Query: 720 FDERNEAVKILLSMAIKAAKAKGAYIGICGQGPSDHADFAAWLVEQGIDTVSLNPDTV 777
+ + A+ L+ M IKAA ++G ++G+CG+ D L+ G+D S++ ++
Sbjct: 475 YQPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGDE-VAIPLLLGLGLDEFSMSATSI 531


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2647TYPE3OMGPROT290.007 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 28.7 bits (64), Expect = 0.007
Identities = 21/97 (21%), Positives = 41/97 (42%), Gaps = 21/97 (21%)

Query: 15 AIEQRINQSEARVIKAVFPSITNHHNTLFGGEALAWMDETAFIAATRFCRKTLVTVSSDR 74
+E + A+V+ P++ N +A+ ET + V V+
Sbjct: 350 LLEN---EGSAQVVSR--PTLLTQENA----QAVIDHSETYY-----------VKVTGKE 389

Query: 75 IDFKKPIPAGTLAELIARVIHVGNTSLKVEVNIFVED 111
+ K I GT+ + RV+ G+ S ++ +N+ +ED
Sbjct: 390 VAELKGITYGTMLRMTPRVLTQGDKS-EISLNLHIED 425


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2648HTHFIS771e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 77.2 bits (190), Expect = 1e-18
Identities = 34/110 (30%), Positives = 51/110 (46%), Gaps = 4/110 (3%)

Query: 8 IIIADDHPLFRNALRQALSSAFEHTQWFEADSADALQSVLDSPTVSYDLVLLDLQMPGSH 67
I++ADD R L QALS A +A L + + DLV+ D+ MP +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDV--RITSNAATLWRWIAA--GDGDLVVTDVVMPDEN 61

Query: 68 GYSTLIHLRSHYPDLPVIVISAHEDINTISRAIHYGGSGFIPKSASMETL 117
+ L ++ PDLPV+V+SA T +A G ++PK + L
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTEL 111


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2653HTHFIS290.001 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.001
Identities = 12/43 (27%), Positives = 18/43 (41%), Gaps = 4/43 (9%)

Query: 1 MERFDRDWHKADIKAALEKAGTNYEKLAEEHGIAGSTLRNALR 43
+ + I AAL N K A+ G+ +TLR +R
Sbjct: 433 LAEMEYPL----ILAALTATRGNQIKAADLLGLNRNTLRKKIR 471


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2664FIMREGULATRY306e-04 Escherichia coli: P pili regulatory PapB protein si...
		>FIMREGULATRY#Escherichia coli: P pili regulatory PapB protein

signature.
Length = 104

Score = 29.9 bits (67), Expect = 6e-04
Identities = 15/62 (24%), Positives = 27/62 (43%)

Query: 4 LLRGAESPERITLMLKMTGIRSPEIIAAIYEHLQFGMREKHAAIKHGVEQQNLNRALNTL 63
LL G+ S L++ ++ I S +I A+ ++L G K K+ + + L L
Sbjct: 23 LLPGSMSEMHFFLLIGISSIHSDRVILAMKDYLVGGHSRKEVCEKYQMNNGYFSTTLGRL 82

Query: 64 NE 65

Sbjct: 83 IR 84


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2688IGASERPTASE337e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 33.5 bits (76), Expect = 7e-04
Identities = 18/84 (21%), Positives = 32/84 (38%), Gaps = 7/84 (8%)

Query: 33 QTQKTESQTVVNSDADKATADAEAKAKLEADTLA------KEQAEQAAKQAAEQQAKDEA 86
+T + Q + A EAK+ ++A+T + ++ ++ A E
Sbjct: 1049 KTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEK 1108

Query: 87 EAKAKADEQDKLNPTNQDGSNEKP 110
E KAK E +K + S P
Sbjct: 1109 EEKAKV-ETEKTQEVPKVTSQVSP 1131



Score = 28.5 bits (63), Expect = 0.022
Identities = 21/120 (17%), Positives = 47/120 (39%), Gaps = 8/120 (6%)

Query: 2 SEPQAKTQTRSRKNAGQSVSTTATVAQDPLLQTQKTESQTVVNSDADKATADAEAKAKLE 61
+ Q + + K+ ++ + T VAQ ++ E+QT + + +AK + E
Sbjct: 1061 TTAQNREVAKEAKSNVKANTQTNEVAQ---SGSETKETQTTETKETATVEKEEKAKVETE 1117

Query: 62 -----ADTLAKEQAEQAAKQAAEQQAKDEAEAKAKADEQDKLNPTNQDGSNEKPLSKNPS 116
++ +Q + + QA+ E + ++ + TN E+P + S
Sbjct: 1118 KTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSS 1177


30SO_2768SO_2784Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_27683141.756218acyl-CoA dehydrogenase
SO_27693171.065618aldose 1-epimerase
SO_27712170.7112832-hydroxy-3-oxopropionate reductase GarR
SO_27721170.778099acyl-CoA thioesterase YciA
SO_27730171.106309predicted inner membrane protein
SO_27740151.0777563-oxoacyl-(acyl-carrier-protein) synthase II
SO_27750150.522533acyl carrier protein AcpP
SO_27761130.7685743-oxoacyl-(acyl-carrier-protein) reductase FabG
SO_2777-1140.576755malonyl CoA-acyl carrier protein transacylase
SO_2778016-0.4083293-oxoacyl-(acyl-carrier-protein) synthase III
SO_2779316-0.052778phosphate:acyl-ACP acyltransferase PlsX
SO_2780521-0.27207350S ribosomal protein L32 RpmF
SO_2781418-0.701868protein of unknown function DUF17
SO_2782318-1.071088Maf septum formation family protein YceF
SO_2784215-0.90876523S rRNA pseudouridine955/2504/2580 synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2775ACRIFLAVINRP250.035 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 25.2 bits (55), Expect = 0.035
Identities = 13/48 (27%), Positives = 18/48 (37%), Gaps = 5/48 (10%)

Query: 34 GADSLDTVELVMALEEEFDTEIPDEEAEKIT-----TVQAAIDYVSKN 76
GA++LDT + + A E P VQ +I V K
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKT 343


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2776DHBDHDRGNASE1421e-43 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 142 bits (358), Expect = 1e-43
Identities = 82/251 (32%), Positives = 131/251 (52%), Gaps = 13/251 (5%)

Query: 7 LAGKVALVTGASRGIGRAIAETLVEAGAVVIGTATSEKGAAAIQEYLGDKGF---GLVLN 63
+ GK+A +TGA++GIG A+A TL GA + + + + L + +
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 64 VTDSQSVTDLFDSIKEKAGDVDILVNNAGITRDNLLMRMKDDEWNDIIDTNLTSLFRLSK 123
V DS ++ ++ I+ + G +DILVN AG+ R L+ + D+EW N T +F S+
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 124 PVMRTMMKKRFGRIINIGSVVGTMGNAGQVNYSAAKAGLIGFTKSLAREVASRQITVNAI 183
V + MM +R G I+ +GS + Y+++KA + FTK L E+A I N +
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 184 APGFIQTDM-----TDELTEDQQ-KAIMSQ----VPMERLGQAQEIANAVLFLASDSAAY 233
+PG +TDM DE +Q K + +P+++L + +IA+AVLFL S A +
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245

Query: 234 ITGETLHVNGG 244
IT L V+GG
Sbjct: 246 ITMHNLCVDGG 256


31SO_2807SO_2819Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2807-115-3.218786c-di-GMP-binding protein
SO_2808014-3.382500protein with CobQ/CobB/MinD/ParA nucleotide
SO_2809018-4.431903ribbon-helix-helix domain protein
SO_2811019-4.092299ISSod4 transposase TnpA_ISSod4
SO_2813015-3.458583oxidoreductase short chain
SO_2815016-4.095555transporter-like protein HCC family
SO_2817-116-3.425728ISSod4 transposase TnpA_ISSod4
SO_2819-118-3.152866ISSod11 transposase TnpA_ISSod11
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2813DHBDHDRGNASE1299e-39 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 129 bits (326), Expect = 9e-39
Identities = 85/257 (33%), Positives = 130/257 (50%), Gaps = 15/257 (5%)

Query: 3 SSNNLQGKVAFVQGGSRGIGAAIVKRLASEGAAVAFTYVSSEAQSQLLVDEVIAQGGKAI 62
++ ++GK+AF+ G ++GIG A+ + LAS+GA +A + E + +V + A+ A
Sbjct: 2 NAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKL-EKVVSSLKAEARHAE 60

Query: 63 AIKADSTEPEAIRRAIRETKAHLGGLDIVVNNAGILIWDSIENLTLEDWERIVNTNVRSV 122
A AD + AI + +G +DI+VN AG+L I +L+ E+WE + N V
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 123 FVASQEAALHMND--GGRIINIGSTNAERIPFVGGAIYGMSKSALVGLAKGLARDLGPRA 180
F AS+ + +M D G I+ +GS N +P A Y SK+A V K L +L
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGS-NPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYN 179

Query: 181 ITVNNIQPGPVDTDMN-----PDNGD------SSEPIKAIGVLGRYGKAEEIASFVAFIA 229
I N + PG +TDM +NG S E K L + K +IA V F+
Sbjct: 180 IRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

Query: 230 GPEAGYITGASLMIDGG 246
+AG+IT +L +DGG
Sbjct: 240 SGQAGHITMHNLCVDGG 256


32SO_2883SO_2894Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2883-113-3.381646protein of unknown function DUF444
SO_2884013-3.730531SpoVR family protein
SO_2885116-4.114493transcriptional regulator of fatty acid
SO_2886215-4.510366sodium:proton antiporter NhaB
SO_2887117-4.350012disulfide bond formation protein DsbB
SO_2889115-3.978137two component signal transduction system
SO_2890-116-0.287820protein of unknown function DUF3478
SO_28910130.131763hypothetical protein
SO_28932131.361791base-induced periplasmic protein YceI
SO_28942141.603568protein of unknown function DUF188 YaiI
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2883CABNDNGRPT330.002 NodO calcium binding signature.
		>CABNDNGRPT#NodO calcium binding signature.

Length = 479

Score = 33.0 bits (75), Expect = 0.002
Identities = 18/51 (35%), Positives = 22/51 (43%), Gaps = 6/51 (11%)

Query: 78 GNDQFTRGDKIDRPQGGSG-----GGAGKGDASDSGEGNDDFVFEISKDEY 123
GND + QGG+G GGAG D G G D FV+ +D
Sbjct: 348 GNDILVGNSADNILQGGAGNDVLYGGAG-ADTLYGGAGRDTFVYGSGQDST 397


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2889RTXTOXIND310.018 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.018
Identities = 9/88 (10%), Positives = 39/88 (44%), Gaps = 8/88 (9%)

Query: 601 RQEKEQEKLLQYQLNQLKD-------QQHKTQLAQQEIAQLRAQLTDANDEIN-LQTQLN 652
+ + ++ + +L+ +H + + + +L ++ +++++
Sbjct: 224 NRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEIL 283

Query: 653 RNKQQSQKLMQHFLSDVMNQMMQEQDRL 680
K++ Q + Q F +++++++ Q D +
Sbjct: 284 SAKEEYQLVTQLFKNEILDKLRQTTDNI 311


33SO_2936SO_3008Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_29362160.321483sialidase superfamily protein
SO_29372180.58619823S rRNA pseudouridine2605 synthase RluB
SO_2938119-1.177882Lambda phage encoded lipoprotein
SO_2939220-0.865313Lambda phage uncharacterized protein
SO_4790221-0.951687Lambda phage uncharacterized protein
SO_2940221-0.941115Lambda phage tail fiber protein J
SO_2941426-2.550230Lambda phage tail assembly protein I
SO_2942424-2.226872Lambda phage transcriptional regulator
SO_2944520-0.252068Lambda phage structural protein
SO_29453180.030891Lambda phage tail fiber protein
SO_2946320-1.457437Lambda phage protein with carbohydrate-binding
SO_2947321-1.036963Lambda phage protein of known function
SO_29482170.634164Lambda phage tail assembly protein K
SO_29492180.191666Lambda phage minor tail protein L
SO_29502190.068672Lambda phage uncharacterized protein
SO_29512190.298773Lambda phage protein of known function
SO_29522242.961581Lambda phage minor tail protein M
SO_29533232.835041Lambda phage tail length tape meausure protein
SO_29543231.133180Lambda phage uncharacterized protein
SO_29553231.255326Lambda phage minor tail protein G
SO_29563230.780076Lambda phage major tail protein V
SO_2957424-0.447232Lambda phage protein of unknown function
SO_2958624-1.104021Lambda phage protein HK97-gp10 family
SO_2959424-0.619209Lambda phage uncharacterized protein
SO_2960323-0.094907Lambda phage phage head-tail joining protein
SO_2961324-0.137252Lambda phage head-tail connector protein
SO_29622231.532045Lambda phage helical domain protein
SO_29631231.914777Lambda phage major capsid protein
SO_29641242.152999Lambda phage head maturation protease
SO_29651241.646396Lambda phage portal protein B
SO_29663251.710660Lambda phage uncharacterized protein
SO_29682262.115224Lambda phage terminase A
SO_2969227-1.061871Lambda phage endonuclease HNH family
SO_2970428-2.730167Lambda phage uncharacterized protein
SO_2971429-3.247516Lambda phage holin S
SO_2972326-1.810475Lambda phage uncharacterized protein
SO_2973326-2.449219Lambda phage lysozyme R
SO_2974427-4.411777Lambda phage pyridoxal phosphate dependent
SO_4791225-1.382621Lambda phage conserved protein
SO_2975225-0.132326Lambda phage uncharacterized protein
SO_29763290.986547Lambda phage uncharacterized protein
SO_47923270.372224Lambda phage uncharacterized protein
SO_29773280.989197Lambda phage uncharacterized protein
SO_29783271.567875Lambda phage integrase
SO_29796262.136301Lambda phage uncharacterized protein
SO_29806221.610796Lambda phage protein
SO_29815211.163221Lambda phage uncharacterized protein
SO_29825231.454171Lambda phage uncharacterized protein
SO_29835251.847919Lambda phage uncharacterized protein
SO_29844241.169020Lambda phage replication protein P
SO_2985220-0.315174Lambda phage replication protein O
SO_2986219-0.336108Lambda phage uncharacterized protein
SO_2987122-0.843928Lambda phage uncharacterized protein
SO_2988121-1.338108Lambda phage phage regulatory protein CII
SO_2989023-2.144176Lambda phage transcriptional repressor of early
SO_2990024-2.250633Lambda phage lytic gene repressor CI
SO_2991126-0.755120Lambda phage lipoprotein
SO_2992329-0.596264Lambda phage protein of unknown function
SO_47931300.708317Lambda phage uncharacterized protein
SO_29931280.517435Lambda phage type II DNA modification
SO_29953340.631826Lambda phage uncharacterized protein
SO_29975290.356979Lambda phage uncharacterized protein
SO_2998629-0.491607Lambda phage uncharacterized protein
SO_29994260.081069Lambda phage protein of unknown function
SO_3000325-0.431993Lambda phage uncharacterized protein
SO_30012261.730040Lambda phage uncharacterized protein
SO_30022262.040951Lambda phage uncharacterized protein
SO_30033312.655676Lambda phage uncharacterized protein
SO_30042292.126793Lambda phage type II restriction-modification
SO_30051270.765598Lambda phage uncharacterized protein
SO_30061260.828455Lambda phage type II restriction modification
SO_4794427-4.986477Lambda phage uncharacterized protein
SO_3007428-6.238371Lambda phage uncharacterized protein
SO_3008427-3.473987Lambda phage uncharacterized protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2940IGASERPTASE360.001 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 35.8 bits (82), Expect = 0.001
Identities = 33/217 (15%), Positives = 59/217 (27%), Gaps = 7/217 (3%)

Query: 1002 ADEALAKSIETVAATIDKNAAAIITEQMARATADESLAKQIISISATVNGNAALIKQEQT 1061
K TV T+++ + T+ S KQ S + A
Sbjct: 1095 TQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVS-PKQEQSETVQPQAEPARENDPTV 1153

Query: 1062 ARADADSALGQRIDTVQA---TTGANTAAIQQEQTARANADSALGQRIDTVQATTGANTA 1118
+ S DT Q T+ + + T T T +
Sbjct: 1154 NIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNS 1213

Query: 1119 AIQQEQTARANADSALGQRIDTVQATTGANTAAIQQEQTARANADSALGQRIDTVQATAG 1178
+ R T+ + + + N ++ L Q A
Sbjct: 1214 ESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVAL 1273

Query: 1179 ANTAAVQQTSTALAELDGKLQAMYSIKVGVTADGKYY 1215
AV Q +++L+ + Y++ V T+ K Y
Sbjct: 1274 NVGKAVSQ---HISQLEMNNEGQYNVWVSNTSMNKNY 1307


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2948PF07520290.023 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 28.8 bits (64), Expect = 0.023
Identities = 20/74 (27%), Positives = 27/74 (36%), Gaps = 14/74 (18%)

Query: 72 PDASSKPSD---RDRAMCEASGLPWHILSWPDGDLRTIVPTGERKSLLNRPFVHGVWDCY 128
P A+S R R + A L +L DG V +P + WD
Sbjct: 503 PTATSVQEQAMIRSR-VSGALTLVKEMLGTKDGTSTIAVEG--------KPELLVDWDEA 553

Query: 129 SCVR--DWYSEVQQ 140
SC + YSE+ Q
Sbjct: 554 SCTQLVYLYSELTQ 567


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2953TYPE4SSCAGX330.006 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 32.8 bits (74), Expect = 0.006
Identities = 43/205 (20%), Positives = 92/205 (44%), Gaps = 21/205 (10%)

Query: 400 EELKALEAERDSVMALMKTERDRSAEQAKRAKLNQDSIEAQRAIAKVTEETLTNEQKRNK 459
E+ KALE E+++ K ++D+ ++ + N+ ++E A + L+N + ++
Sbjct: 143 EQKKALEKEKEAKEQAQKAQKDKREKRKEERAKNRANLE-NLTNAMSNPQNLSNNKNLSE 201

Query: 460 AIKEYNDN-------IEKVRKADPNSALLNADKIKRDLA--SIEEKFKDSAKTTKAFADD 510
IK+ +N +E +++ +AL +++ + A ++ ++ KD +
Sbjct: 202 LIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVRQRAKDKISIKTDKSQK 261

Query: 511 AATSYLMRLRETQAGLQGQLDRNTKLTQSQKELLQFEQQIADIKNKDVLTAQQKSLLAEQ 570
+ + L + + + L + ++ K L QF I I KD + ++ E
Sbjct: 262 SPEDNSIELSPSDSAWRTNL-----VVRTNKALYQF---ILRIAQKDNFASAYLTVKLEY 313

Query: 571 SVIRAQLEKNVALDEELKKRNEALR 595
+ E + ++EELKKR EA R
Sbjct: 314 P---QRHEVSSVIEEELKKREEAKR 335


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2962IGASERPTASE270.010 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 27.3 bits (60), Expect = 0.010
Identities = 11/54 (20%), Positives = 23/54 (42%)

Query: 41 LVELAPEQATEADAKADAEAKEKEEADAKAAAEAKEKEEADAKAAAAAAKKAKA 94
V +Q ++ K + +A E + + A EAK +A+ + A ++
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSET 1092


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2963GPOSANCHOR290.039 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 28.9 bits (64), Expect = 0.039
Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 1/59 (1%)

Query: 22 DQIKSAAEETNKQIKASGEMHAETRDKVDKLLSEQGALQARLQEAEQKLLKGAQSNQQE 80
Q++ A EE N ++ A +++ E + E+ LQA+L EAE K LK + Q E
Sbjct: 396 KQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKL-EAEAKALKEKLAKQAE 453


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2970MALTOSEBP290.006 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 28.5 bits (63), Expect = 0.006
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 1/42 (2%)

Query: 6 VIFNGKIVSVP-AVESYIDNGEKKLVPLVPADWVEVTALNAA 46
V +NGK+++ P AVE+ K L+P P W E+ AL+
Sbjct: 123 VRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKE 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2973CHLAMIDIAOMP300.004 Chlamydia major outer membrane protein signature.
		>CHLAMIDIAOMP#Chlamydia major outer membrane protein signature.

Length = 393

Score = 30.0 bits (67), Expect = 0.004
Identities = 9/25 (36%), Positives = 17/25 (68%)

Query: 83 SLPNVELNQASYDLYIDWTYQYGIG 107
S+PN+ L+Q+ +LY D + + +G
Sbjct: 172 SVPNMSLDQSVVELYTDTAFSWSVG 196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4792ACETATEKNASE270.010 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 26.7 bits (59), Expect = 0.010
Identities = 7/15 (46%), Positives = 12/15 (80%)

Query: 5 VKCGSSSIEFQFVED 19
+ CGSSS+++Q +E
Sbjct: 6 INCGSSSLKYQLIES 20


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2987PREPILNPTASE290.001 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 28.6 bits (64), Expect = 0.001
Identities = 9/32 (28%), Positives = 17/32 (53%), Gaps = 5/32 (15%)

Query: 15 HIKAALSVRQ--PTLTF---TGKCHYCKAPVS 41
H ++ + P L++ G+C C+AP+S
Sbjct: 76 HCNHPITALENIPLLSWLWLRGRCRGCQAPIS 107


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2991PF00577280.012 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 28.3 bits (63), Expect = 0.012
Identities = 22/93 (23%), Positives = 35/93 (37%), Gaps = 15/93 (16%)

Query: 1 MKLITASLLLALLTGCATSAVTIDKAKPAPAERV-----LIGNTDSADAKITIIRDSGFM 55
K A + L CA +A P + + + + A A ++ + +
Sbjct: 19 RKHRLAGFFVRLFVACAFAA-----QAPLSSAELYFNPRFLADDPQAVADLSRFENGQEL 73

Query: 56 GGGCY-VDVYVNDALAAKLDTAEKVTFNVRSGE 87
G Y VD+Y+N+ A D VTFN E
Sbjct: 74 PPGTYRVDIYLNNGYMATRD----VTFNTGDSE 102


34SO_3073SO_3091Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_30730173.341947bifunctional 5-methylaminomethyl-2-thiouridine
SO_30750173.005326RNA methyltransferase TrmH family
SO_30761172.694888protein YfcL
SO_30771162.490634ATP-NAD kinase
SO_3078-211-0.493674transporter major facilitator superfamily
SO_3079017-5.780933chorismate synthase AroC
SO_3080019-6.72341750S subunit L3 protein glutamine
SO_3081221-7.472006UPF0115 family protein YfcN
SO_3082223-7.841047phosphohistidine phosphatase SixA
SO_3083020-6.381761Zn-dependent peptidase subfamily M16A
SO_3084-121-5.676682bifunctional diguanylate
SO_30850160.964510predicted periplasmic protein
SO_3086092.048682hypothetical protein
SO_3087092.341251predicted membrane protein
SO_30880102.554101anaerobic fatty oxidation complex alpha subunit
SO_30890123.244353anaerobic fatty oxidation complex beta subunit
SO_30900153.322130MoxR-like ATPase in aerotolerance operon
SO_30911153.129106protein of unknown function DUF58 in
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3078TCRTETA424e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 41.7 bits (98), Expect = 4e-06
Identities = 60/352 (17%), Positives = 112/352 (31%), Gaps = 40/352 (11%)

Query: 47 GFLLAILMATRIVAPNVWAKVADRTGMRSELIKMGAGAAMLAYLSFFYHGGFVYMALSLA 106
G LLA+ + V ++DR G R L+ AGAA+ + MA +
Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI----------MATAPF 95

Query: 107 LYTFFWNAILAQLEVITLETLGENASRYGQIRSFGS----IGYICLVVGAGF----AIGQ 158
L+ + I+A IT T + I G++ G G +G
Sbjct: 96 LWVLYIGRIVAG---ITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGG 152

Query: 159 WGTEVLPYIGLVLFTGMLVCALPLPANRAVRPQGQERQPLKWT--------------KPI 204
P+ + ER+PL+ +
Sbjct: 153 LMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVV 212

Query: 205 IWFMISAMLLQMSAGPFYGFFVLYLKQA-GYTEAAAGI-FVALGAMAEIVMFMFAPRLLG 262
M ++Q+ +V++ + + GI A G + + M +
Sbjct: 213 AALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAA 272

Query: 263 RYGVNTLLVVSIAMTAVRWLLVAFGVESMLLLGLSQVLHAFTFGLTHAASIQFVHRHFDA 322
R G L++ + ++L+AF + + +L + G+ A + R D
Sbjct: 273 RLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGM--PALQAMLSRQVDE 330

Query: 323 SHRSQGQALYASLSFGVGGALGTWICGYIWGDGSGAVWSWVFAATCAFAAML 374
+ Q Q A+L+ + +G + I+ W + A A +
Sbjct: 331 ERQGQLQGSLAALT-SLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLC 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3090HTHFIS346e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 6e-04
Identities = 35/139 (25%), Positives = 53/139 (38%), Gaps = 19/139 (13%)

Query: 28 LIALIANG--HLLVEGPPGLAKT---RAVKALCDGVEGDFHRIQ---FTPDLLPADLTG- 78
++A + L++ G G K RA+ G F I DL+ ++L G
Sbjct: 152 VLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGH 211

Query: 79 -----TDIYRSQTGTFEFEAGPIFHNLILADEINRAPAKVQSALLEAMAEGQVT-VGKNS 132
T TG FE G + DEI P Q+ LL + +G+ T VG +
Sbjct: 212 EKGAFTGAQTRSTGRFEQAEG----GTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRT 267

Query: 133 YKLPPLFLVMATQNPLENE 151
+ +V AT L+
Sbjct: 268 PIRSDVRIVAATNKDLKQS 286


35SO_4798SO_3204Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4798-118-3.413873low complexity protein of unknown function
SO_3157-117-3.646897polysaccharide biosynthesis lipoprotein WbfD
SO_3158-117-3.467320polysaccharide synthesis-related protein of
SO_3159-217-3.310108outer membrane protein of unknown function
SO_3160017-3.572621dTDP-4-keto-L-rhamnose-35-epimerase RfbC
SO_3162-117-3.541891two component signal transduction system
SO_3163117-4.044160outer membrane lipoprotein NlpE
SO_3164018-4.324206putative histidine degradation protein HutD
SO_3165-122-3.700850toxin-antitoxin system antidote Mnt family
SO_3166-124-3.503363toxin-antitoxin system toxin HepN family
SO_3167-226-3.778516dTDP-glucose-4,6-dehydratase RffG
SO_3168024-3.604950DnaJ domain protein
SO_3169027-3.913362toxin-antitoxin system antidote transcriptional
SO_3170-129-4.822475toxin-antitoxin system toxin HipA family
SO_3171-130-6.151822polysaccharide biosynthesis protein
SO_3172-133-7.883149galactosyl transferase WbfU
SO_3173-134-8.659883NAD-dependent epimerase/dehydratase family
SO_3174038-11.127228O-antigen biosynthesis glycosyl transferase
SO_3175139-11.655871asparagine synthase glutamine-hydrolyzing WbpQ
SO_3176141-13.499091O-antigen biosynthesis glycosyl transferase
SO_3177142-13.846807formyltransferase domain protein
SO_3178144-14.232973polysaccharide deacetylase
SO_3179144-14.666546O-antigen polymerase Wzy
SO_4799244-14.415309O-antigen biosynthesis acetyltransferase WbnJ
SO_3180242-14.180775glycosyl transferase family 2 WbnI
SO_3181240-13.125722O-antigen flippase Wzx
SO_3182135-11.287226O-antigen biosynthesis acetyltransferase WbnH
SO_3183137-10.618535perosamine synthetase-related protein
SO_3184132-8.667035putative glycine transferase in O-antigen
SO_3185130-7.331437enzyme for biosynthesis of dTDP-Qui4N
SO_3186129-6.650271glucose-1-phosphate-thymidylyltransferase RmlA
SO_3188025-5.003160dTDP-glucose-4,6-dehydratase RfbB
SO_3189-124-5.201471UDP-GlkcNAc C4 epimerase WbpP
SO_3190-122-4.060259UDP-N-acetyl-d-glucosamine 6-dehydrogenase WbpA
SO_3191-120-3.713987polysaccharide chain length determinant Wzz
SO_3192-118-3.510214hypothetical protein
SO_3193-118-3.295415outer membrane polysaccharide export channel
SO_3194119-3.532244transcriptional antiterminator RfaH
SO_3195118-3.307805proton:peptide symporter POT family
SO_3196019-3.665396two component signal transduction system
SO_3197-122-3.638467phospholipid transport-associated protein MlaA
SO_3198022-3.297893putative cytoplasmic protein in chemotaxis
SO_3199220-2.425386FlhB domain protein
SO_3200225-1.718257putative membrane anchored protein of unknown
SO_3202321-0.976831chemotaxis signal transduction system adaptor
SO_3203321-1.083313chemotaxis signal transduction system adaptor
SO_3204220-0.961289ParA family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4798OMPADOMAIN250.044 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 24.9 bits (54), Expect = 0.044
Identities = 13/37 (35%), Positives = 20/37 (54%)

Query: 1 MKKTSLALIAAMSMLGSVAANANTTGTTTGGAGAGGA 37
MKKT++A+ A++ +VA A T GA G +
Sbjct: 1 MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWS 37


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3162PF06580514e-09 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 51.0 bits (122), Expect = 4e-09
Identities = 35/198 (17%), Positives = 74/198 (37%), Gaps = 38/198 (19%)

Query: 266 NTMQDGLGLIERNLTRAAELV--------HNFKRTAADQSVLERERFNLKAYLFQIFSSL 317
N + + LI + T+A E++ ++ + + A Q L E + +YL + S
Sbjct: 177 NALNNIRALILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQ-LAS-- 233

Query: 318 RPLMR-KKNIALTVELDEQIFIESYPGAIAQIFTNLVANSFRHGFPEHFTGDKKITIRVE 376
++ + + +++ I P + Q LV N +HG KI ++
Sbjct: 234 ---IQFEDRLQFENQINPAIMDVQVPPMLVQT---LVENGIKHGI-AQLPQGGKILLKGT 286

Query: 377 KQGSNICMQYQDNGVGMTDEVKIKAFEPFFTTARKDGGTGLGMSIIYNLVTQKLHG---S 433
K + ++ ++ G K TG G+ + + Q L+G
Sbjct: 287 KDNGTVTLEVENTGSLALKNTK--------------ESTGTGLQNVRERL-QMLYGTEAQ 331

Query: 434 ILLTSQPNQGVTIDIQLP 451
I L+ + + + + +P
Sbjct: 332 IKLSEKQGKVNAM-VLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3167NUCEPIMERASE1874e-59 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 187 bits (477), Expect = 4e-59
Identities = 79/355 (22%), Positives = 137/355 (38%), Gaps = 51/355 (14%)

Query: 1 MRILVTGGAGFIGSALVRMLIEQTESVVL--NFDKLTYASHPESLAGVADNERYHFVQAD 58
M+ LVTG AGFIG + + L+E VV N + S ++ + + F + D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 59 ICDRARLEQVLQQFQPDLMMHLAAESHVDRSIDGPAEFIQTNIVGTYTLLEACRSYYQTL 118
+ DR + + + + V S++ P + +N+ G +LE CR
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ- 119

Query: 119 GQAQQRRFRLHHISTDEVFGSLTETGLFSETSAYD-PSSPYSASKASADHLVRAWHRTYA 177
L + S+ V+G L FS + D P S Y+A+K + + + + Y
Sbjct: 120 --------HLLYASSSSVYG-LNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170

Query: 178 LPIVITNCSNNYGPFQYPEKLIPLMVSNALQSKPLPIYGNGQQVRDWLYVDDHVKALYLV 237
LP YGP+ P+ + L+ K + +Y G+ RD+ Y+DD +A+ +
Sbjct: 171 LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRL 230

Query: 238 ------------------ATQGQLGQTYNIGGSCEQTNLTVVRHICSLLEELVPTHPQSL 279
A + YNIG S + + LE+ +
Sbjct: 231 QDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYI----QALEDAL------- 279

Query: 280 AMGNAGFADLIQYVVDRPGHDVR--YAIDASKIQRELGWRPQESFESGLRKTVEW 332
G + +PG DV A D + +G+ P+ + + G++ V W
Sbjct: 280 -----GIEAKKNMLPLQPG-DVLETSA-DTKALYEVIGFTPETTVKDGVKNFVNW 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3171NUCEPIMERASE523e-09 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 52.1 bits (125), Expect = 3e-09
Identities = 47/298 (15%), Positives = 101/298 (33%), Gaps = 44/298 (14%)

Query: 283 VMVTGAGGSIGSELCRQILKQLPKQLVLFELSEFALYSIERELSATATELGIDVEIVPIM 342
+VTGA G IG + +++L+ + + + L+++ S+++ + G
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQF----HK 58

Query: 343 GSVQRENRVQAVMQAFKVQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYTARAAIAAKV 402
+ + + + + V+ + V N +N+ G L K+
Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118

Query: 403 ETFVLVST---------------DKAVRPTNVMGTTKRMAELALQALAKENHHTRFCMVR 447
+ + S+ D P ++ TK+ EL + + +R
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYS-HLYGLPATGLR 177

Query: 448 FGNVLGSSGS---VVPLFRKQIANGGPVTV-THPEITRFFMTIPEASQLVIQA------- 496
F V G G + F K + G + V + ++ R F I + ++ +I+
Sbjct: 178 FFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHA 237

Query: 497 -----------GAMGKGGDVFVLDMGKSVKIIDLAAKMIRLSGYDVKDEANP--NGDI 541
A V+ + V+++D + G + K P GD+
Sbjct: 238 DTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGDV 295


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3173NUCEPIMERASE692e-15 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 68.7 bits (168), Expect = 2e-15
Identities = 63/347 (18%), Positives = 116/347 (33%), Gaps = 65/347 (18%)

Query: 5 SILLTGATGFVGQQILRQLPQDT-RVFG----------RTKPAR-------DCHFFAGEL 46
L+TGA GF+G + ++L + +V G K AR F +L
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 47 TANTDYRSAL---SGVDVVIHCAARAHVMNETANNAAQLYQEVNTLVTLALAEQAAAAGV 103
A+ + + L + V R V N A Y + N L + E +
Sbjct: 62 -ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHA--YADSNLTGFLNILEGCRHNKI 118

Query: 104 KRFIFISTIKVNGEATIAGQLFRASD-ARQPLDHYGESKAKAEIGLFDIARKTEIEVVII 162
+ ++ S+ V G F D P+ Y +K E+ + + +
Sbjct: 119 QHLLYASSSSVYGLNRKMP--FSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGL 176

Query: 163 RPPLVYGPNVKANFATMLNLAKKNL----PLPFGAIHNKRSMVALDNLVDLIVTCIEHPN 218
R VYGP + + A + K L + KR +D++ + I+ +
Sbjct: 177 RFFTVYGPWGRPDMA-LFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIP 235

Query: 219 AANQ-----------------IFLVSDDQDVSTTELLKLMTGAAGKKPRLLPVPMAWLIL 261
A+ ++ + + V + ++ + A G + + +P+
Sbjct: 236 HADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQ---- 291

Query: 262 AGKVTGNQAIIDRLCGNLQVDITHTKNTLSWQPPITVEEGVRRCFVK 308
G V A D + + P TV++GV+ FV
Sbjct: 292 PGDVLETSA-----------DTKALYEVIGFTPETTVKDGVKN-FVN 326


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3182SACTRNSFRASE383e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 38.4 bits (89), Expect = 3e-06
Identities = 18/59 (30%), Positives = 27/59 (45%)

Query: 70 AYLSMLAVDPEYRGKGFAKKLILDMESTVRDNGFKTIRLEVYKTNEGALSMYLKLNYII 128
A + +AV +YR KG L+ ++N F + LE N A Y K ++II
Sbjct: 90 ALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3188NUCEPIMERASE1754e-54 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 175 bits (446), Expect = 4e-54
Identities = 82/376 (21%), Positives = 140/376 (37%), Gaps = 66/376 (17%)

Query: 1 MKILVTGGAGFIGSAVVRHIIGNTQDCVVNVDKLT--YAGNLESLT-SVADSPRYTFEKV 57
MK LVTG AGFIG V + ++ VV +D L Y +L+ + P + F K+
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQ-VVGIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59

Query: 58 DICDRTELERVFSLHQPDAVMHLAAESHVDRSITGSADFIQTNIVGTYTLLEAARHYWMQ 117
D+ DR + +F+ + V V S+ + +N+ G +LE RH +Q
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 118 LNTERKSAFRFHHISTDEVYGDLPHPDEINVECSMLNDECKDHSTLNIQHSTLPLFTETT 177
+ S+ VYG +P T+ +
Sbjct: 120 ---------HLLYASSSSVYGLNRK---------------------------MPFSTDDS 143

Query: 178 PYTPSSPYSASKASSDHLVRAWLRTYGFPTIVTNCSNNYGPYHFPEKLIPLVILNALEGK 237
P S Y+A+K +++ + + YG P YGP+ P+ + LEGK
Sbjct: 144 VDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGK 203

Query: 238 PLPIYGKGDQIRDWLYVEDHARALFKVV------------------TEGKVGETYNIGGH 279
+ +Y G RD+ Y++D A A+ ++ YNIG
Sbjct: 204 SIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNS 263

Query: 280 NEKRNLEVVQTICSILDSLVPKNTPYAEQIAYVADRPGHDRRYAIDATKMSAELDWQPQE 339
+ ++ +Q + L KN + +PG + D + + + P+
Sbjct: 264 SPVELMDYIQALEDALGIEAKKN--------MLPLQPGDVLETSADTKALYEVIGFTPET 315

Query: 340 TFETGLRKTVEWYLAN 355
T + G++ V WY
Sbjct: 316 TVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3189NUCEPIMERASE2595e-87 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 259 bits (663), Expect = 5e-87
Identities = 94/339 (27%), Positives = 160/339 (47%), Gaps = 30/339 (8%)

Query: 19 LITGVAGFIGSNLLEQLLKLNQTVIGLDNFATGRQHNLDEVQSLVTSEQWMRFSFINGDI 78
L+TG AGFIG ++ ++LL+ V+G+DN +L + + + ++ F F D+
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQP--GFQFHKIDL 61

Query: 79 RDYAICEAVV--NGVDYVLHQAALGSVPRSIADPITTNAANITGFLNMLQAAKEAEVKSF 136
D + + V +V S+ +P +N+TGFLN+L+ + +++
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 137 TYAASSSTYGDHPALP-KVEQNIGNPLSPYAVTKYVNELYASVYARTYGFETIGLRYFNV 195
YA+SSS YG + +P + ++ +P+S YA TK NEL A Y+ YG GLR+F V
Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFTV 181

Query: 196 FGRRQDPNGAYAAVIPKWTSSMIKGEDVFINGDGETSRDFCYIDNVVQMNILAA------ 249
+G P+ A K+T +M++G+ + + G+ RDF YID++ + I
Sbjct: 182 YGPWGRPDMAL----FKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHA 237

Query: 250 ----------TAASEAKNEVYNVAVGDRTTLNDLYFAIKDSLNANGINVNQNPNYRDFRA 299
AAS A VYN+ L D A++D+L + N +
Sbjct: 238 DTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDAL-----GIEAKKNMLPLQP 292

Query: 300 GDVRHSQADVSKAVTRLGYQYTHKILEGISEAMPWYKEF 338
GDV + AD +G+ + +G+ + WY++F
Sbjct: 293 GDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3196HTHFIS944e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 93.7 bits (233), Expect = 4e-23
Identities = 36/154 (23%), Positives = 62/154 (40%), Gaps = 2/154 (1%)

Query: 7 SILWVEDDPVFRQIVATFLSGRGAQVVQAGDGEQGLIHFKQQRFDIILADLSMPKLGGLD 66
+IL +DD R ++ LS G V + D+++ D+ MP D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 67 MLKEMSKLEPLVPSIVISGNNVMADVVEALRIGACDYLVKPVADLFIIEQAIQQGLQRHQ 126
+L + K P +P +V+S N ++A GA DYL KP DL + I + L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF-DLTELIGIIGRALAEPK 123

Query: 127 LDDISQTDLDVLSHQELSDNLTILEQSVEAAKQV 160
S+ + D L +++ ++
Sbjct: 124 R-RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3197VACJLIPOPROT2292e-77 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 229 bits (584), Expect = 2e-77
Identities = 88/224 (39%), Positives = 128/224 (57%), Gaps = 4/224 (1%)

Query: 42 EDPRDPFEGFNRVMWDFNYLYLDRYLYRPVAHGYNDYIPLPAKMGVNNFLQNLEEPSSVV 101
+ DP EGFNR M++FN+ LD Y+ RPVA + DY+P PA+ G++NF NLEEP+ +V
Sbjct: 26 QGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMV 85

Query: 102 NNLLQGKWGWAANAGGRFTVNTTIGLLGVIDVADMMGMTRKQDE---FNEVLGYYGVPNG 158
N LQG RF +NT +G+ G IDVA M ++ E F LG+YGV G
Sbjct: 86 NYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYG 145

Query: 159 PYFMAPFAGPYIVRELASDWVDGLYFPLSELTMWQSVLKWGLKSLHARASAIDQERLVDN 218
PY PF G + +R+ D D LY LS LT SV KW L+ + RA +D + L+
Sbjct: 146 PYVQLPFYGSFTLRDDGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQ 205

Query: 219 ALDPYAFVKDAYIQHMDYKVYDGNV-PQKQEDDELLDQYMQELE 261
+ DPY V++AY Q D+ G + PQ+ + + + +++++
Sbjct: 206 SSDPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQDDLKDID 249


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3199TYPE3IMSPROT561e-12 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 55.5 bits (134), Expect = 1e-12
Identities = 18/87 (20%), Positives = 32/87 (36%), Gaps = 3/87 (3%)

Query: 7 TQQAVALSYD-GKH-APKVVASGEGLVADEIIALAKASGVYIHQDPHLSNFL-RLLELGE 63
T A+ + Y G+ P V + +A+ GV I Q L+ L +
Sbjct: 265 THIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDH 324

Query: 64 EIPRELYLLIAELIAFVYMLDGKFPEQ 90
IP E AE++ ++ + +
Sbjct: 325 YIPAEQIEATAEVLRWLERQNIEKQHS 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3203PF03544290.018 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 29.2 bits (65), Expect = 0.018
Identities = 16/88 (18%), Positives = 28/88 (31%)

Query: 79 KSIVTVSTKENAEPLVNKQALERLLAPVLKTQAPDIPKPTELNEQPLPLPKPVEAIAVTN 138
S+ V+ + P + E ++ P + + P P PKP
Sbjct: 50 ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVK 109

Query: 139 VVKPADVESQQVEIISTAPETQVGFAPP 166
V+ + + VE +P A P
Sbjct: 110 KVEQPKRDVKPVESRPASPFENTAPARP 137


36SO_3244SO_3272Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3244219-0.919513flagellar component of cell-distal portion of
SO_3245218-1.618188flagellar component of cell-proximal portion of
SO_3247118-2.832775flagellar hook protein FlgE
SO_3248-120-4.214846flagellar hook assembly protein FlgD
SO_3249-120-4.399000flagellar basal-body rod protein FlgC
SO_3250122-4.698685flagellar basal-body rod protein FlgB
SO_3251122-4.453690chemotaxis signal transduction system MCP
SO_3252122-3.976461chemotaxis signal transduction system response
SO_3253023-3.349009assembly protein for flagellar basal-body P ring
SO_3254118-3.319697flagellar biosynthesis anti-sigma factor FlgM
SO_3255117-3.103060secretion chaperone for FlgK and FlgL FlgN
SO_3256016-2.937990outer membrane lipoprotein required for
SO_3257118-3.103572outer membrane lipoprotein required for
SO_3258220-3.230391flagella assembly protein FlgT
SO_3259218-3.348535*motility accessory factor Maf
SO_3260220-2.671422hypothetical protein
SO_3261222-3.953437polysaccharide biosynthesis related-protein
SO_3262323-4.220931TPP-dependent enzyme involved in flagella
SO_3263321-3.9866123-oxoacyl-(acyl-carrier-protein) reductase
SO_3264321-4.106111SAM-dependent methyltransferase in
SO_3265424-4.130513hypothetical protein
SO_3266322-4.179128SAM-dependent methyltransferase in
SO_3267319-3.252219SAM-dependent methyltransferase
SO_3268218-3.841101flagellin modification glycoside hydrolase
SO_3269218-4.235598flagellin modification protein
SO_3270016-4.052855C4 aminotransferase for PseB product PseC
SO_3271014-3.324298bifunctional UDP GlcNAc C6 dehydratase/C5
SO_3272-114-3.397008ISSod6 transposase TnpA_ISSod6
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3244FLGHOOKAP1452e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.6 bits (105), Expect = 2e-07
Identities = 19/119 (15%), Positives = 41/119 (34%), Gaps = 4/119 (3%)

Query: 145 EDATSITVSAEGEVSVKTPGAAENQVVGQLSMTDFINPSGLDPMGQNLYTETG---ASGT 201
D I +++E + + + Q + + +L ++ G A+
Sbjct: 427 TDEAKIAMASEEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLK 486

Query: 202 PIQGTASLDGMGAIRQGALETSNVNVTEELVNLIESQRIYEMNSKVISAVDQMLAYVNQ 260
T + + S VN+ EE NL Q+ Y N++V+ + + +
Sbjct: 487 TSSATQGNV-VTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544



Score = 36.5 bits (84), Expect = 9e-05
Identities = 9/36 (25%), Positives = 21/36 (58%)

Query: 5 LWISKTGLDAQQTDISVISNNVANASTVGYKKSRAV 40
+ + +GL+A Q ++ SNN+++ + GY + +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI 39


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3247FLGHOOKAP1401e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 40.3 bits (94), Expect = 1e-05
Identities = 15/35 (42%), Positives = 22/35 (62%)

Query: 2 SFNIALSGISAAQKDLNTTANNIANANTIGFKESR 36
N A+SG++AAQ LNT +NNI++ N G+
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37



Score = 37.2 bits (86), Expect = 1e-04
Identities = 13/49 (26%), Positives = 25/49 (51%)

Query: 405 SLSSSALEQSNIDLTTELVDLISAQRNFQANSRTLEVNNTLQQTVLQIR 453
LS+ S ++L E +L Q+ + AN++ L+ N + ++ IR
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3249FLGHOOKAP1333e-04 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 33.0 bits (75), Expect = 3e-04
Identities = 9/38 (23%), Positives = 18/38 (47%)

Query: 99 NVNVMEEMADMISASRSYQMNVQVAEAAKSMLQQTLGM 136
VN+ EE ++ + Y N QV + A ++ + +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 28.4 bits (63), Expect = 0.010
Identities = 15/64 (23%), Positives = 27/64 (42%), Gaps = 6/64 (9%)

Query: 8 DVAGSGMSAQSLRLNTTASNIANADSVSSSIDKTYRSRHPIFEAEMAKAQSQQQASQGVA 67
+ A SG++A LNT ++NI++ + Y + I + + GV
Sbjct: 5 NNAMSGLNAAQAALNTASNNISSYN------VAGYTRQTTIMAQANSTLGAGGWVGNGVY 58

Query: 68 VKGI 71
V G+
Sbjct: 59 VSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3252HTHFIS612e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 60.6 bits (147), Expect = 2e-12
Identities = 23/128 (17%), Positives = 53/128 (41%), Gaps = 12/128 (9%)

Query: 180 HIMVIDDSAVARKQIIRSLESLNLQIDTAKDGREALDKLKAIASEMNNVADEIPLIISDI 239
I+V DD A R + ++L + + + A + L+++D+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAA---------GDGDLVVTDV 55

Query: 240 EMPEMDGYTLTAEIRDDPKLKNIKVVLHTSLSGVFNQAMVQKVGANDFIAK-FNPDELAA 298
MP+ + + L I+ ++ V++ ++ + + GA D++ K F+ EL
Sbjct: 56 VMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG 113

Query: 299 AVNKHLSL 306
+ + L+
Sbjct: 114 IIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3256FRAGILYSIN270.029 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 27.0 bits (59), Expect = 0.029
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)

Query: 1 MKRYLLVIAALLLTGCAAK-DKYVEWEDVPPTSFPKLTAIGYAPLATQ 47
+K L++ A LL C+ + D D P T+ L ++ Y LATQ
Sbjct: 12 VKLLLMLGTAALLAACSNEADSLTTSIDAPVTASIDLQSVSYTDLATQ 59


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3263DHBDHDRGNASE913e-24 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 91.3 bits (226), Expect = 3e-24
Identities = 59/253 (23%), Positives = 105/253 (41%), Gaps = 12/253 (4%)

Query: 3 KLVLITGGSRGIGAGIAKAFAEAGYWVAITYLNH--QDKAVSLANILGDKVAAFALDQSK 60
K+ ITG ++GIG +A+ A G +A N +K VS AF D
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 61 PESIKQCITEVEKYFNRSIDVLINNGAIAQEKPFSDITADDFTTMLNTNLRGPFLLAQAC 120
+I + +E+ ID+L+N + + ++ +++ + N G F +++
Sbjct: 69 SAAIDEITARIEREMGP-IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 121 IPAMQQHGFGRIINIGSIGGQWGGYNQVHYAAAKAGLINLSQSIAKIYSRDGIRTNTIAI 180
M G I+ +GS + YA++KA + ++ + + IR N ++
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 181 GLVATEMTEHELTTEAGKQKAAA---------IPVGRLGKVEDIASIALFLASQDSDYLS 231
G T+M E G ++ IP+ +L K DIA LFL S + +++
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHIT 247

Query: 232 GQTLNANGGMYFG 244
L +GG G
Sbjct: 248 MHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3264LPSBIOSNTHSS300.008 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 30.2 bits (68), Expect = 0.008
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 7/68 (10%)

Query: 59 IAVYTNPGRQGLPSSAKIRDLDEQIQFIKRGIGHLPKSAMQIGSSDGYTLSRFRQAGVEL 118
+AV NP +Q + S + E+++ I + I HLP + + +R RQAG +
Sbjct: 32 VAVLRNPNKQPMFS------VQERLEQIAKAIAHLPNAQVDSFEGLTVNYARQRQAGA-I 84

Query: 119 VMGVEPGS 126
+ G+ S
Sbjct: 85 LRGLRVLS 92


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3271NUCEPIMERASE803e-19 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 80.2 bits (198), Expect = 3e-19
Identities = 43/245 (17%), Positives = 86/245 (35%), Gaps = 54/245 (22%)

Query: 6 TILITGGTGSFGQKYTKTILERY-----------------KPKRLIIFSRDELKQYEMQQ 48
L+TG G G +K +LE K RL + ++ + ++
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHK--- 58

Query: 49 VFNAPCMRYFIGDVRDGERLKQAFKDVDF--VIHAAALKQVPAAEYNPMECIKTNIHGAE 106
D+ D E + F F V + V + NP +N+ G
Sbjct: 59 -----------IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFL 107

Query: 107 NVIRAAISNNVKKVIALST---------------DKAASPINLYGATKLASDKLFVAANN 151
N++ N ++ ++ S+ D P++LY ATK A++ + ++
Sbjct: 108 NILEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH 167

Query: 152 VVGDGKTRFAAVRYGNVVGSRGS---VVPFFKQLIANGATSLPITHPDMTRFWITLQDGV 208
+ G +R+ V G G + F + + G + + M R + + D
Sbjct: 168 LYG---LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIA 224

Query: 209 DFVLK 213
+ +++
Sbjct: 225 EAIIR 229


37SO_3293SO_3340Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3293-118-3.933450inosine-5-monophosphate dehydrogenase GuaB
SO_3294-123-5.703527exodeoxyribonuclease VII large subunit XseA
SO_3296031-7.119285ISSod12 transposase TnpA_ISSod12
SO_3297131-6.524589transcriptional regulator LysR family
SO_3298229-5.585786outer membrane beta barrel protein
SO_3299230-5.569519phenylalanine/histidine ammonia-lyase family
SO_3300329-5.885998flavocytochrome c heme submit
SO_3301225-4.720252flavocytochrome c flavin subunit
SO_3302222-4.171342extracellular peptidase family S8A
SO_3303219-4.052005cell surface protein
SO_3305116-3.083532two component signal transduction system
SO_3306114-1.987596two component signal transduction system hybrid
SO_33082161.53300650S ribosome assembly GTPase Der
SO_33091130.773840beta barrel protein translocation lipoprotein
SO_33102120.815605membrane anchored protein with DUF2133 domain
SO_33112111.196065histidyl-tRNA synthetase HisS
SO_33121101.6331134-hydroxy-3-methylbut-2-en-1-yl diphosphate
SO_3313-1101.868627transmembrane cell shape protein RodZ
SO_3314-1121.946734outer membrane PilQ pilotin PilF
SO_3315-2110.80919123S rRNA (adenine2503-C2)-methyltransferase
SO_3316-1120.659647protein of unknown function DUF21 with CBS
SO_3317015-0.0534985'-nucleotidase
SO_3318019-1.719407transcriptional regulator LysR family
SO_3319-121-3.449005membrane protein DoxX family
SO_3321-124-4.405807ISSod11 transposase TnpA_ISSod11
SO_3323-218-2.870742acteyltransferase GNAT family
SO_3324018-2.973747acteyltransferase GNAT family
SO_3325017-2.272452periplasmic protein of unknown function NrfJ
SO_3326018-3.107776hypothetical protein
SO_3328019-2.893202acteyltransferase GNAT family
SO_3331021-2.602394protein with CobQ/CobB/MinD/ParA nucleotide
SO_3332018-2.824898transcriptional regulator CopG family
SO_3333-116-2.536039periplasmic substrate binding protein family 1
SO_3334-117-3.099325diguanylate cyclase with HAMP domain
SO_3335-117-2.707611periplasmic protein of unknown function DUF980
SO_3336-215-3.096570predicted secreted protein
SO_3337-215-2.926877signal transduction protein with HDOD/GAF
SO_3338-117-3.023043threonine aldolase YbjU
SO_3339-117-4.117302outer membrane porin OmpA family
SO_3340-118-3.447273small conductance mechanosensitive ion channel
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3301PF07520310.013 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 31.1 bits (70), Expect = 0.013
Identities = 14/68 (20%), Positives = 29/68 (42%), Gaps = 2/68 (2%)

Query: 330 AQLAVLASGTKEKPNMPFVFCGEATANHAEGFKAAYRDGAIKKSETLEELAKRYDVDINA 389
Q+A+ + + + + +V A + F+ GA+ S L+ L D +
Sbjct: 148 VQIALDTALSDQDQSAHYVAPERADSEKPREFRLVSDPGAM--SWFLQRLEADEDGNAVD 205

Query: 390 LQNSINEW 397
LQ +++W
Sbjct: 206 LQLWVSDW 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3302SUBTILISIN1073e-27 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 107 bits (269), Expect = 3e-27
Identities = 60/278 (21%), Positives = 99/278 (35%), Gaps = 76/278 (27%)

Query: 161 KVIKETPTELQTDNGPQLIGASNLWDGNATGLAAKGDGIIIGILDTGINTDNRAFSAVGD 220
+VIK+ + G ++I A +W+ +G G+ + +LDTG + D+
Sbjct: 11 QVIKQEQQVNEIPRGVEMIQAPAVWNQT------RGRGVKVAVLDTGCDADHPDLK---- 60

Query: 221 DGHNIINPLGSGNYLGDCVKDATLCNDKLIGVYSFPLVTDEYNGLRPANGEDYNGHGSHT 280
++IG +F + + P +DYNGHG+H
Sbjct: 61 --------------------------ARIIGGRNF----TDDDEGDPEIFKDYNGHGTHV 90

Query: 281 ASTAAGNALVNVPVLMPNIGEEVGDGIETGTVLSNISGVAPHANIISYQVCDQSGCYP-S 339
A T A G + GVAP A+++ +V ++ G
Sbjct: 91 AGTIAATE--------NENG---------------VVGVAPEADLLIIKVLNKQGSGQYD 127

Query: 340 LTIASVELAIKAGVDVLNYSIGPRGGVQNDPWNTASDIAFLSAREAGIFVAMAAGNAGPD 399
I + AI+ VD+++ S+G V A A + I V AAGN G
Sbjct: 128 WIIQGIYYAIEQKVDIISMSLGGPEDV------PELHEAVKKAVASQILVMCAAGNEGDG 181

Query: 400 AETVGNV-----APWAISVAASSHQRVWSHVLS-GSGV 431
+ + ISV A + R S + + V
Sbjct: 182 DDRTDELGYPGCYNEVISVGAINFDRHASEFSNSNNEV 219



Score = 71.0 bits (174), Expect = 5e-15
Identities = 32/132 (24%), Positives = 51/132 (38%), Gaps = 33/132 (25%)

Query: 578 DILADFSSRGPYKWQTELMVPHIAAPGVDIYAAYADEMPFTSVNDAAPSDFAFLSGTSMA 637
++FS+ + APG DI + +A SGTSMA
Sbjct: 207 RHASEFSNSNNE--------VDLVAPGEDILSTVPG------------GKYATFSGTSMA 246

Query: 638 SPHVAGSAALLRQL-----HPDWTPAEIQSAMMLTATTNVLKEDGKTPAGIFDIGSGRLQ 692
+PHVAG+ AL++QL D T E+ + ++ G +P G+G L
Sbjct: 247 TPHVAGALALIKQLANASFERDLTEPELYAQLIKRTIP-----LGNSP---KMEGNGLLY 298

Query: 693 IDKAAQAGLVMD 704
+ + + D
Sbjct: 299 LTAVEELSRIFD 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3305HTHFIS493e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 49.4 bits (118), Expect = 3e-09
Identities = 26/120 (21%), Positives = 53/120 (44%), Gaps = 5/120 (4%)

Query: 6 HVMIADDHPLYLDALVNGLVSHLPGTQVSQANNYIELFDSLYLQVEEIDLLIMDLFMPGS 65
+++ADD L L G V +N L+ ++ + DL++ D+ MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRA--GYDVRITSNAATLWR--WIAAGDGDLVVTDVVMPDE 60

Query: 66 SGYAGLSFLRTQFPTLPIVVISALDDLIARSQCIQHGA-AFISKSTAPTNIFKQVEQILD 124
+ + L ++ P LP++V+SA + + + + GA ++ K T + + + L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3306PF06580310.035 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.6 bits (69), Expect = 0.035
Identities = 15/107 (14%), Positives = 38/107 (35%), Gaps = 18/107 (16%)

Query: 935 SLLLRRVIDNILSNAIKISDPDTSVSLSVCQERQHAVIEVIDQGPGMTQQMQAELFTPFK 994
+L++ +++N + + I + L ++ +EV + G + +
Sbjct: 257 PMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK-------- 308

Query: 995 RWTSRYQGSGLGLS-VVKGIADLLG--ISLSIRSTLGEGTQFTLKLP 1038
+ +G GL V + + L G + + G + +P
Sbjct: 309 ------ESTGTGLQNVRERLQMLYGTEAQIKLSEKQG-KVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3308TCRTETOQM330.003 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 32.9 bits (75), Expect = 0.003
Identities = 38/159 (23%), Positives = 67/159 (42%), Gaps = 35/159 (22%)

Query: 199 IKLAIIGKPNVGKSTLTNRIL----GEERVVVYDEPGTTRDSIYIPMER----------- 243
I + ++ + GK+TLT +L + D+ T D+ + +R
Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSF 63

Query: 244 --DGREYVIIDTAGVRRRSKVHEVIEKFSVIKTLKAVEDANVVLLIIDAREGVAEQDLGL 301
+ + IIDT G + + E V ++L ++ A +L+I A++GV Q L
Sbjct: 64 QWENTKVNIIDTPG-----HMDFLAE---VYRSLSVLDGA---ILLISAKDGVQAQTRIL 112

Query: 302 LGFALNAGRALVIAVNKWD--GID-----QGIKDRVKSE 333
G + +NK D GID Q IK+++ +E
Sbjct: 113 FHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAE 151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3316RTXTOXINA300.018 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 29.9 bits (67), Expect = 0.018
Identities = 23/102 (22%), Positives = 46/102 (45%), Gaps = 19/102 (18%)

Query: 23 AVLLSVTP-SYIATLDQTDSAA-----AARLRKLKENIEAPLV-----------SILTLN 65
AV L+++P S+++ D+ A + R +KL + ++ L S+ T++
Sbjct: 313 AVTLAISPLSFLSIADKFKRANKIEEYSQRFKKLGYDGDSLLAAFHKETGAIDASLTTIS 372

Query: 66 TVAHTVGAAVAGAQAAKVFGDDMLGVFSGVLTFI--ILFFSE 105
TV +V + ++ A + G + + V I IL S+
Sbjct: 373 TVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASK 414


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3324SACTRNSFRASE280.012 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 28.4 bits (63), Expect = 0.012
Identities = 15/79 (18%), Positives = 35/79 (44%), Gaps = 3/79 (3%)

Query: 75 GASLTKICAGFINVETDFRHRGYIDSLYIHPDWQRQGLGELAYRQLEQWARAQGYSQL-- 132
L C G I + +++ I+ + + D++++G+G + +WA+ + L
Sbjct: 69 LYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLML 128

Query: 133 -STDASYLSRGLFIKLGFI 150
+ D + + + K FI
Sbjct: 129 ETQDINISACHFYAKHHFI 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3339OMPADOMAIN731e-17 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 73.0 bits (179), Expect = 1e-17
Identities = 32/118 (27%), Positives = 51/118 (43%), Gaps = 12/118 (10%)

Query: 90 VYFEFAIAEVDLSQWKALALVKSFLEAN--TETKLTLVGHTDIVGTPEFNYQLSLQRAQN 147
V F F A + AL + S L + + ++G+TD +G+ +N LS +RAQ+
Sbjct: 221 VLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQS 280

Query: 148 VKRILVEDYGFNPNRFTVVGKGISEPVADNRSSEGRGL---------NRRVQFIVNNI 196
V L+ G ++ + G G S PV N + +RRV+ V I
Sbjct: 281 VVDYLISK-GIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGI 337


38SO_3368SO_3374Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3368-119-3.944608adenine glycosylase MutY
SO_3369-126-6.491881Fe(II) trafficking protein YggX
SO_3370-225-5.689428UPF0312 family alkali-inducible periplasmic
SO_3371-123-5.641291cytochrome B561 YceJ
SO_3373-120-5.338359****hypothetical protein
SO_3374-116-3.477749membrane protein of unknown function DUF3634
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3369adhesinmafb270.016 Neisseria meningitidis: adhesin MafB signature.
		>adhesinmafb#Neisseria meningitidis: adhesin MafB signature.

Length = 467

Score = 26.6 bits (58), Expect = 0.016
Identities = 23/80 (28%), Positives = 35/80 (43%), Gaps = 12/80 (15%)

Query: 12 KEADGLDFQLYPGDLGKRIFDNISKEAWG----------LWQKKQTMLINEKKLNMMNVD 61
K + LD DL +R D SK G L Q K+T+ +K N +N
Sbjct: 362 KYREALDIHY--EDLIRRKTDGSSKFINGREIDAVTNDALIQAKRTISAIDKPKNFLNQK 419

Query: 62 DRKFLEAQMTSFLFEGKDVE 81
+RK ++A + + +GK E
Sbjct: 420 NRKQIKATIEAANQQGKRAE 439


39SO_3507SO_3526Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3507-219-3.195732N-acetylglucosamine kinase NagK
SO_3509-120-4.034422beta-N-acetylhexosaminidase HexB
SO_3512-123-5.672360SapC family protein
SO_3513022-5.109203flavin-dependent tryptophan halogenase
SO_3514022-5.059652TonB-dependent chitooligosaccharide receptor
SO_3516221-4.931672transcriptional repressor of N-acetylglucosamine
SO_3517318-4.678592respiratory NADH dehydrogenase II Ndh
SO_3518219-5.186347ISSod4 transposase TnpA_ISSod4
SO_3519223-5.781993regulatory protein for nitrogen assimilation by
SO_3520429-6.196793type IV minor pilin protein FimT
SO_3521330-5.807115type IV pilus biogenesis protein FimU
SO_3522329-5.844037ISSod4 transposase TnpA_ISSod4
SO_3523433-6.022452type IV pilin system protein
SO_3524331-5.185526type IV minor pilin protein PilE
SO_3525230-4.767600type IV pili system adhesin PilY
SO_3526024-3.470422type IV minor pilin protein PilX
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3514ACRIFLAVINRP310.034 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 30.6 bits (69), Expect = 0.034
Identities = 33/168 (19%), Positives = 65/168 (38%), Gaps = 31/168 (18%)

Query: 32 AEATAAAPENIEKIEVRGMRASMKASVNDKRFSDSVVDAVTAEDIGKFPDGDVGESLARI 91
AT P+ +++ + ++S + SD+ T +DI + +V ++L+R+
Sbjct: 112 QLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDN--PGTTQDDISDYVASNVKDTLSRL 169

Query: 92 PGVAVNRQFGQGQQVSIRGASNQLTRTLLNGHTVASTGWFDQQAIDRSFNYSLLPPELVG 151
GV + FG + I W D + Y L P +++
Sbjct: 170 NGVGDVQLFGAQYAMRI---------------------WLDADLL---NKYKLTPVDVIN 205

Query: 152 GILVNKSSQADIAEGGVGGTITVK-TRKPLDLEANSLFLSAKGDYGTV 198
+ K IA G +GGT + + + A + F + + ++G V
Sbjct: 206 QL---KVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNPE-EFGKV 249


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3520BCTERIALGSPG290.006 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 28.7 bits (64), Expect = 0.006
Identities = 13/49 (26%), Positives = 25/49 (51%), Gaps = 3/49 (6%)

Query: 5 QYGFSLIELITTLSISTLLISIGAPTYT---DITDHIRADSNIKTIQQT 50
Q GF+L+E++ + I +L S+ P + D +A S+I ++
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENA 55


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3521BCTERIALGSPG347e-05 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 34.5 bits (79), Expect = 7e-05
Identities = 13/27 (48%), Positives = 19/27 (70%)

Query: 6 KGFTLVELMVTIAIAALLLSVGVPSFT 32
+GFTL+E+MV I I +L S+ VP+
Sbjct: 8 RGFTLLEIMVVIVIIGVLASLVVPNLM 34


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3524BCTERIALGSPG589e-14 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 57.6 bits (139), Expect = 9e-14
Identities = 23/63 (36%), Positives = 41/63 (65%)

Query: 4 KDKGFTLIEVMIVVVIIGILSAIAYPSYTRYVAQSTRAEGLSALMKLANLQEQYYLDNRK 63
K +GFTL+E+M+V+VIIG+L+++ P+ ++ + + +S ++ L N + Y LDN
Sbjct: 6 KQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHH 65

Query: 64 YAT 66
Y T
Sbjct: 66 YPT 68


40SO_3537SO_3546Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3537-222-3.15822530S ribosomal protein S20 RpsT
SO_3538-217-2.727028transcriptional activator HlyU
SO_3539-114-1.822219peptidase M28D family
SO_3540119-1.864562protein of unknown function DUF328
SO_3541221-1.774754alanine/glycine:cation symporter (AGCS) family
SO_3542323-1.980429D-xylulose 5-phosphate/D-fructose 6-phosphate
SO_3543421-1.551778ISSod13 transposase TnpA_ISSod13
SO_3544321-2.474723ISSod7 transposase TnpA_ISSod7
SO_3545425-3.130950outer membrane porin
SO_3546216-2.849951transaldolase B TalB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3545OMPADOMAIN1576e-47 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 157 bits (397), Expect = 6e-47
Identities = 86/376 (22%), Positives = 142/376 (37%), Gaps = 56/376 (14%)

Query: 2 MKNTLK--VVLLTSMLPLAASASQELTPWYVGAGLGVNNYEHIATDNGD----DNPYAWD 55
MK T V L +A +A ++ T WY GA LG + Y N + +N
Sbjct: 1 MKKTAIAIAVALAGFATVAQAAPKDNT-WYTGAKLGWSQYHDTGFINNNGPTHENQLGAG 59

Query: 56 IFAGYMFNDYFGAEIGYRDLGSADWTTGGISNDAGVKGATLGLVGVWPLGNRWSLSAEAG 115
F GY N Y G E+GY LG + + +G L +P+ + + G
Sbjct: 60 AFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLG 119

Query: 116 AMYYTLENSQHTGTTSSSYSSNDFAPYVGAGVGYNFTDNLKLQAKYRRYENLDDTDFNTI 175
M + + + +P GV Y T + + +Y+ N+ D
Sbjct: 120 GMVWRADTKSNV---YGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGT 176

Query: 176 EADSNYWGLELSYRFGTPAAAAPVAAAVVAAAPVDSDNDGVYDDKDECPATPATHKVDSV 235
D+ L +SYRFG AA VA A A V + + + D
Sbjct: 177 RPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSD---------------- 220

Query: 236 GCTLYENVKKQEDVGSIQFANDSAVVKKEYYKDIERLANYM--NKNPEFTVEIAGHASNV 293
+ F + A +K E +++L + + + +V + G+ +
Sbjct: 221 ----------------VLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRI 264

Query: 294 GKPEYNMVLSDKRADAVAKILVEKYGISQSRVTSNGYGITKPLVAGNS----------KE 343
G YN LS++RA +V L+ K GI ++++ G G + P V GN+ +
Sbjct: 265 GSDAYNQGLSERRAQSVVDYLISK-GIPADKISARGMGESNP-VTGNTCDNVKQRAALID 322

Query: 344 AHAANRRIEAIVTTTE 359
A +RR+E V +
Sbjct: 323 CLAPDRRVEIEVKGIK 338


41SO_3576SO_3587Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3576214-0.751819predicted periplasmic protein
SO_3577215-0.686401stress-induced multi-chaperone system component
SO_3578213-1.483612uncharacterized protein YfiH
SO_3579213-1.60861023S rRNA pseudouridine1911/1915/1917 synthase
SO_3580214-2.367965beta barrel protein translocation lipoprotein
SO_3582218-2.415821***methyl-accepting chemotaxis sensory transducer
SO_3583013-1.83622316S rRNA pseudouridine synthase RsuA family
SO_3584012-1.822378superoxide-responsive transcriptional repressor
SO_3585119-3.043823NADPH-dependent azoreductase Azr
SO_3586120-3.738704glyoxalase family protein
SO_3587017-3.263787hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3577HTHFIS443e-06 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 43.7 bits (103), Expect = 3e-06
Identities = 35/180 (19%), Positives = 66/180 (36%), Gaps = 30/180 (16%)

Query: 552 LEGEREKLLQMEVALHER--VIGQNEAVDAVANAIRRSRAGLADPNRPIGSFLFLGPTGV 609
L + + ++E + ++G++ A+ + + R L + + + G +G
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLAR----LMQTDLTL---MITGESGT 171

Query: 610 GKTELCKSLARFLFDSESALVRIDMSEFMEKHAVSRLVGAPPGYVGYEEGGYLTEAVRRK 669
GK + ++L + V I+M+ S L G +E+G + T A R
Sbjct: 172 GKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFG-------HEKGAF-TGAQTRS 223

Query: 670 PYSV-------ILLDEVEKAHPDVFNILLQVLDDG---RLTDGQGRTVDFRNTVIIMTSN 719
+ LDE+ D LL+VL G + D R I+ +N
Sbjct: 224 TGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN 280



Score = 30.2 bits (68), Expect = 0.040
Identities = 14/68 (20%), Positives = 29/68 (42%), Gaps = 3/68 (4%)

Query: 151 DPNAEDQRQALKKFTVDLTERAEQG-KLDPVIGRDDEIRRTIQVLQRRTKNN-PVLI-GE 207
+AL + ++ + P++GR ++ +VL R + + ++I GE
Sbjct: 109 TELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGE 168

Query: 208 PGVGKTAI 215
G GK +
Sbjct: 169 SGTGKELV 176


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3582CHANLCOLICIN310.016 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 30.8 bits (69), Expect = 0.016
Identities = 29/241 (12%), Positives = 81/241 (33%), Gaps = 8/241 (3%)

Query: 266 IAEAANTVTSSATELSSFTQETNKRMQQQQAETEQTATAMNEMTATVAEVAQSTSAAANS 325
++ A + V E+ + + + + AE + A NE+ A+ +
Sbjct: 198 LSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKL 257

Query: 326 AKDADTYAA-----NGNRIVIDSISSMSQLSEQIQKTAQVIGFLSNESQNIGRVLDVIKS 380
+ A+ R + + + +Q+ + I ++ + I + + + +
Sbjct: 258 SPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSN 317

Query: 381 IAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRTLAQRTQKSTQEIEAMIATLQQGVKQ 440
+ ++ A E + + + + D V Q T++ + + Q +
Sbjct: 318 NRNAG-IARVHEAEENLKKAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQEL-- 374

Query: 441 AVSAMEVGIKQVDDANNKANQAGQALKEIVASVDNIAELNTHIATAAEEQSSVAENINRS 500
A + I V++A + L + + D A N + ++ + + +
Sbjct: 375 ADKSKGKKIGNVNEALAAFEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKY 434

Query: 501 I 501
+
Sbjct: 435 L 435


42SO_3617SO_3627Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3617116-3.381735hypothetical protein
SO_3618018-4.810932hypothetical protein
SO_3621019-4.869580periplasmic protein of unknown function DUF3299
SO_3622-121-5.533769periplasmic RmlC-type Cupin domain family
SO_3623-220-4.273387flavocytochrome c heme submit
SO_3625-218-3.672717ISSod3 transposase TnpA_ISSod3
SO_3627018-3.633626transcriptional repressor of flavocytochrome c
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3627HTHTETR562e-11 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 55.8 bits (134), Expect = 2e-11
Identities = 24/143 (16%), Positives = 53/143 (37%), Gaps = 3/143 (2%)

Query: 15 QLLDTAEQLIDEQGVVSFRFAQIAKKSECSTNTLYKYFESKEDVL-ACLFLRNTTSIQIP 73
+LD A +L +QGV S +IAK + + +Y +F+ K D+ L + ++
Sbjct: 15 HILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELE 74

Query: 74 IFINENPNLTIHEHTLLPILFTFEAIKRSPIFNVLRVVSINSMFWQLASTQKIDVLKNRV 133
+ ++ E+ +L + + + + + +
Sbjct: 75 LEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCE-FVGEMAVVQQAQRNL 133

Query: 134 NL-FWSRIKTPLEDAVKEGELKA 155
L + RI+ L+ ++ L A
Sbjct: 134 CLESYDRIEQTLKHCIEAKMLPA 156


43SO_3670SO_3676Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_36702151.950735TonB1 energy transduction system for heme uptake
SO_36712152.028988TonB1 energy transduction system for heme uptake
SO_36722162.332566TonB1 energy transduction system for heme uptake
SO_36733132.753868ABC-type hemin uptake system substrate-binding
SO_36742142.513448ABC-type hemin uptake system permease component
SO_36752152.003216ABC-type hemin uptake system ATPase component
SO_36762191.463476protein of unknown function DUF2956
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3670PF03544585e-12 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 57.7 bits (139), Expect = 5e-12
Identities = 16/79 (20%), Positives = 34/79 (43%), Gaps = 4/79 (5%)

Query: 196 PQPTYPRMARKKGLEGTATIEVMFNEFGQQLALTLVKSSGVSLLDQAALEAVETWQFEAP 255
QP YP A+ +EG ++ G+ + ++ + ++ ++ A+ W++E
Sbjct: 163 NQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG 222

Query: 256 SPKLASHYKVRVPIRFALN 274
P + V I F +N
Sbjct: 223 KP----GSGIVVNILFKIN 237


44SO_3865SO_3890Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3865225-5.581071ABC-type molybdate uptake system ATPase
SO_3866432-7.926574site-specific recombinase phage integrase
SO_3867540-9.719764transcriptional regulator Cro/CI family
SO_3870434-8.722449disulfide bond formation protein DsbB family
SO_3871325-7.147579thiol:disulfide interchange protein DsbA family
SO_3872118-5.654453arylsulfate sulfotransferase AssT
SO_3873013-4.321112nucleoside-specifc outer membrane porin Tsx
SO_3874-111-2.209679transcriptional regulator LysR family
SO_38761140.128147ISSod4 transposase TnpA_ISSod4
SO_38781161.061607ISSod5 transposase TnpA_ISSod5
SO_38803170.994822ISSod13 transposase TnpA_ISSod13
SO_38822180.934760ISSod4 transposase TnpA_ISSod4
SO_38842171.082249site-specific recombinase phage integrase
SO_38851160.138700AAA ATPase family protein
SO_3886-215-2.051171hypothetical protein
SO_3887-213-3.669844protein of unknown function DUF2787
SO_3888-113-3.138119protein of unknown function DUF1508
SO_3890-116-3.695354*energy taxis-modulating methyl accepting sensory
45SO_3925SO_3937Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_39252241.579887Fe hydrogenase maturation rSAM protein HydE
SO_3926224-0.799022Fe hydrogenase maturation GTPase HydF
SO_3927127-0.68273750S ribosomal protein L9 RplI
SO_39280160.08865930S ribosomal protein S18 RpsR
SO_39290101.026522primosomal replication protein N PriB
SO_3930090.87700030S ribosomal protein S6 RpsF
SO_39311110.920278outer membrane protein of unknown function
SO_39331111.706143transport protein MFS superfamily
SO_39342181.12035323S rRNA (guanosine2251-2'-O)-methyltransferase
SO_39353210.7515703'-5' exoribonuclease R Rnr
SO_3936225-0.735169flagellar rotation associated protein MotX
SO_39372250.480708adenylosuccinate synthetase PurA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3933TCRTETB462e-07 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 46.4 bits (110), Expect = 2e-07
Identities = 30/162 (18%), Positives = 56/162 (34%), Gaps = 1/162 (0%)

Query: 221 APAYASNLGLPPEKVATYMTATILAGLLAQWPMGKLSDIMSRSRLIRINCVLLGILALGI 280
P A++ PP TA +L + GKLSD + RL+ ++ ++
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 281 ALTPYHPTVSLVMTFLFGILGFTFYPLATALANSRVEQSERVGLSATILLTFGMGASIGP 340
+ ++ ++ F+ G F L + + + R I MG +GP
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 341 LIASTLMQWFGNSMLYGFMSACTVILFLRLRYVHSQQKAETN 382
I + + S L T+I L + ++
Sbjct: 157 AIGGMIAHYIHWSYLLLIPMI-TIITVPFLMKLLKKEVRIKG 197



Score = 31.0 bits (70), Expect = 0.010
Identities = 25/137 (18%), Positives = 55/137 (40%), Gaps = 12/137 (8%)

Query: 213 IVGSFYGLAPAYASNLGLPPEKVATYMTATILAGLLAQWPMGKLSDIMSRSRLIRINCVL 272
++ + L+ A ++ + P + I+ G + G L D ++ I
Sbjct: 282 MMKDVHQLSTAEIGSVIIFPG-----TMSVIIFGYIG----GILVDRRGPLYVLNIGVTF 332

Query: 273 LGILALGIALTPYHPTV--SLVMTFLFGILGFTFYPLATALANSRVEQSERVGLSATILL 330
L + L + + ++++ F+ G L FT ++T +++S +Q G+S
Sbjct: 333 LSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT 392

Query: 331 TFGMGASIGPLIASTLM 347
+F + G I L+
Sbjct: 393 SF-LSEGTGIAIVGGLL 408


46SO_3987SO_4008Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3987112-4.268909protein of unknown function DUF3293
SO_3988012-4.219851two component signal transduction system
SO_3990012-4.624643periplasmic dipeptidylpeptidase IV Dpp4
SO_3991118-6.229527fructose-16-bisphosphatase Fbp
SO_3993119-4.290901hypothetical protein
SO_3994116-2.681927hypothetical protein
SO_3996014-0.691493predicted inner membrane protein
SO_3997-114-0.484210membrane protein UPF0114 family
SO_4000-1140.183234ISSod3 transposase TnpA_ISSod3
SO_4002-1150.433279two component signal transduction system hybrid
SO_40033202.982416metal dependent phosphohydrolase with response
SO_40042202.777999proton/sodium:glutamate symporter DAACS family
SO_40054212.896660protein of unknown function DUF2220
SO_40064192.842930hypothetical protein
SO_40072192.129686hypothetical protein
SO_40082171.862760putative recombination regulator protein DUF3584
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3988HTHFIS832e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 82.6 bits (204), Expect = 2e-20
Identities = 31/131 (23%), Positives = 61/131 (46%), Gaps = 1/131 (0%)

Query: 1 MQNPHILIVEDEAVTRNTLRSIFEAEGYVVTEANDGAEMHKAMQENKINLVVMDINLPGK 60
M IL+ +D+A R L GY V ++ A + + + +LVV D+ +P +
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 NGLLLARELREIN-NIGLIFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLT 119
N L +++ ++ ++ ++ ++ + I E GA DY+ KPF+ EL L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 120 RVNSAGNEVEE 130
+++E+
Sbjct: 121 EPKRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4002HTHFIS696e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 69.5 bits (170), Expect = 6e-14
Identities = 26/132 (19%), Positives = 55/132 (41%), Gaps = 2/132 (1%)

Query: 1352 LLLVEDNYLNQELAVELLRQAGARVTVAQHGQEALTLLAQQSFDCVLMDGQMPVMDGYEA 1411
+L+ +D+ + + + L +AG V + + +A D V+ D MP + ++
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 1412 TRLIRAQPQFADLPIIAMTANAMDSDRERALAAGMNAQINKPFQVQQLYSTIAQHVSVHS 1471
I+ DLP++ M+A +A G + KPF + +L I + ++
Sbjct: 66 LPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 1472 VQSLPESPEAHD 1483
+ ++ D
Sbjct: 124 RRPSKLEDDSQD 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4003HTHFIS763e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.6 bits (186), Expect = 3e-17
Identities = 40/161 (24%), Positives = 69/161 (42%), Gaps = 16/161 (9%)

Query: 6 TKPIVLVVDDSADNIQILHGLLSD-KYSIRAATSGAKALALAAIEPMPDLILLDVMMPEM 64
T +LV DD A +L+ LS Y +R ++ A A DL++ DV+MP+
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD-GDLVVTDVVMPDE 60

Query: 65 DGFQTCIRLK-HNPLTRHIPIIFVTAKTDIVDERTGFELGAVDYISKPVKPAILEARVKT 123
+ F R+K P +P++ ++A+ + E GA DY+ KP
Sbjct: 61 NAFDLLPRIKKARP---DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDL-------TE 110

Query: 124 HLTLASRANQLESLVQLRTQELESARYKIIHKLGRAAEFRD 164
+ + RA + R +LE + +GR+A ++
Sbjct: 111 LIGIIGRALAEP---KRRPSKLEDDSQDGMPLVGRSAAMQE 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4008GPOSANCHOR528e-09 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 52.4 bits (125), Expect = 8e-09
Identities = 53/316 (16%), Positives = 102/316 (32%), Gaps = 10/316 (3%)

Query: 598 EYAASEQELRIRLSKAEEALQSAQELQAEAESQLVAINGELDKLSRELTFARTAYKNSRD 657
EL LS A+E L+ + +E S++ + L + L A
Sbjct: 82 ALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSA 141

Query: 658 DLRRLFDEKRSEQDKINKALAERKAFAQQRLTQLDGELKQLKHQHQLWLEEQKEQALEAR 717
++ L EK + KA K + + E ++ LE
Sbjct: 142 KIKTLEAEK---AALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 198

Query: 718 MEKQAYWQEVIGALDNQLGQIKATIDARRESAKAEQKACETWYKNELKSRGVDEDNILKL 777
+E + A L KA + AR+ + + + + E L
Sbjct: 199 LEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 258

Query: 778 KQQIRELETKISRAEQRRSEVLRFDDWY-----QHTWLIRKPKLQTQLSDVKR-AASEID 831
+ + ELE + A + + Q+Q+ + R +
Sbjct: 259 EARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL 318

Query: 832 QQLKAKTQEVKTRRQQLETERKASDAAQIEASENLTKLRAVMRKLAELKLPANNEEAQGS 891
+ ++++ Q+LE + K S+A++ +L R ++L E + E+ + S
Sbjct: 319 DASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL-EAEHQKLEEQNKIS 377

Query: 892 LGERLRQGEDLLLKRD 907
R DL R+
Sbjct: 378 EASRQSLRRDLDASRE 393



Score = 34.7 bits (79), Expect = 0.002
Identities = 38/348 (10%), Positives = 104/348 (29%), Gaps = 27/348 (7%)

Query: 374 QTEKHQDIEAAYNARRSKIGEQLNRELEGLHSEQDKQREARDKQREVARGDLDALEAQWR 433
E + K+ E+ ++ ++ + K + + L+
Sbjct: 37 TNEVSAVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKA--------LKDHND 88

Query: 434 SQMDAGKASFSEQEYQFKLNAAELKLRVDGVTYTEEEKLNLAIFDERIHRADEEQESCNA 493
+ + + K + + + + + L + ++ A
Sbjct: 89 ELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEA 148

Query: 494 KVERLTSDERKLRAKRDQANEALRIASLRVNERQTALDEL---------HHMLFPQSHTL 544
+ L + + L + A S ++ + L T
Sbjct: 149 EKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTA 208

Query: 545 LEFLRKEAQGWEQSLGKVIAPELLHRTDLHPSVTGESDTVFGVNLDLKAID--------- 595
K + + +L A T +S + + + A++
Sbjct: 209 DSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 268

Query: 596 VPEYAASEQELRIRLSKAEEALQSAQELQAEAESQLVAINGELDKLSRELTFARTAYKNS 655
+ ++ E + + +A+ E Q +N L R+L +R A K
Sbjct: 269 LEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQL 328

Query: 656 RDDLRRLFDEKRSEQDKINKALAERKAFAQQRLTQLDGELKQLKHQHQ 703
+ ++L ++ + + ++L +++ QL+ E ++L+ Q++
Sbjct: 329 EAEHQKLEEQNKI-SEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375


47SO_4028SO_4068Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_40280143.115136single-strand binding protein Ssb
SO_40290142.692786drug:H+ antiporter DHA1 family
SO_40300183.033889excinuclease ABC A subunit UvrA
SO_4031-1161.411872predicted transmembrane protein
SO_4032-1171.250393M28 family peptidase
SO_40340171.521308ATP-dependent RNA helicase DeaD
SO_40350150.378280predicted membrane protein
SO_40360140.700361hypothetical protein
SO_40373130.188891predicted lipoprotein
SO_40383151.092758hypothetical protein
SO_40393140.311842hydrolase haloacid dehalogenase-like family
SO_4040213-1.58614210 TMS drug/metabolite efflux pump (DME) family
SO_4042213-0.824461hydratase/decarboxylase family protein
SO_4043111-0.442095TonB mediated energy transduction system energy
SO_40440140.765387hypothetical protein
SO_4045-1141.472636hypothetical protein
SO_4046-1152.533331hypothetical protein
SO_4047-1163.646040SoxA-like diheme cytochrome c
SO_40480183.446772diheme cytochrome c4
SO_4050-1183.623087putative DUF2955 domain transport system
SO_40510193.857276putative DUF2955 domain transport system MFP
SO_40520203.787012transcriptional regulator SlyA
SO_4053-1203.879687methyl-accepting chemotaxis protein
SO_4054-1234.0402355,10-methylenetetrahydrofolate reductase MetF
SO_4055-1234.298640bifunctional aspartokinase II/homoserine
SO_40561214.065631cystathionine gamma-synthase MetB
SO_40570213.186880transcriptional repressor of methionine
SO_40581202.9054174-toluene sulfonate uptake permease family
SO_40600212.604644sulfur reductase membrane anchoring component
SO_4061017-2.934352sulfur reductase FeS subunit PhsB
SO_4062015-3.589597sulfur reductase molybdoperterin-binding subunit
SO_4064118-6.160020pentapeptide repeat protein McbG
SO_4814122-4.342843hypothetical protein
SO_4066121-4.313650phosphoribosylaminoimidazole-succinocarboxamide
SO_4068020-5.141015hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4028PERTACTIN343e-04 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 34.3 bits (78), Expect = 3e-04
Identities = 25/74 (33%), Positives = 30/74 (40%), Gaps = 4/74 (5%)

Query: 140 AAPAQNQYAPAPQAAPAYQAPAPQPQSGYNQPPAQQSYGQQQAQPHVQPHAQPQQGGYAP 199
AA Q++ AP PAPQP P Q Q QP P QP+ AP
Sbjct: 553 AANGNGQWSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPE----AP 608

Query: 200 KPAAPAYQAPAAPA 213
P PA + +A A
Sbjct: 609 APQPPAGRELSAAA 622


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4029TCRTETA795e-18 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 78.7 bits (194), Expect = 5e-18
Identities = 75/368 (20%), Positives = 135/368 (36%), Gaps = 29/368 (7%)

Query: 11 KKVAFSLASVFGLRMMGLFMIMPV--FALYGQHLEGFSPLWVGIAIGAYGLTQAVLQIPM 68
+ + L++V L +G+ +IMPV L GI + Y L Q +
Sbjct: 5 RPLIVILSTVA-LDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVL 63

Query: 69 GILSDKYGRKPVILAGLVVFAIGSVIAANAETIYGVVFGRAVQGM-GAIAAAVLALAADL 127
G LSD++GR+PV+L L A+ I A A ++ + GR V G+ GA A A AD+
Sbjct: 64 GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADI 123

Query: 128 TRDEQRTKVMAIIGMCIGLSFALSLLMGPIVAQHLGLTGLFWLTALLAILGMLLIQFLVP 187
T ++R + + C G ++G ++ F+ A L L L FL+P
Sbjct: 124 TDGDERARHFGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLP 182

Query: 188 NPITQAPKGDTLATPAKLKRMFFEPQLFRLNAGIFILHLVLTAVFVALPLDLVDAGL--- 244
+ L FR G+ ++ ++ F+ + V A L
Sbjct: 183 ESHKGERRPLRREALNPLAS-------FRWARGMTVVAALMAVFFIMQLVGQVPAALWVI 235

Query: 245 --VKEKHW-------MLYFPAFVGAFF-LMVPLIIIGVKRKNTKAMFQIALVIMMFALAA 294
HW L + + M+ + + M + + L A
Sbjct: 236 FGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLA 295

Query: 295 MAIFASNLWVLSVAVLLFFTGFNYLEASLPSLIAKFCPVGEKGSAMGVYSTSQFLGAFCG 354
FA+ W+ +++ +L +++++ +G G + L + G
Sbjct: 296 ---FATRGWMA-FPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVG 351

Query: 355 GMLGGGAF 362
+L +
Sbjct: 352 PLLFTAIY 359


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4043PF03544684e-15 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 67.7 bits (165), Expect = 4e-15
Identities = 28/85 (32%), Positives = 40/85 (47%), Gaps = 5/85 (5%)

Query: 285 QPLFRTAPDYPMSYARQAKNGWVQLKFTVDEHGFVKNTEILASKGGALFEKESIEALNKW 344
+ L R P YP G V++KF V G V N +IL++K +FE+E A+ +W
Sbjct: 158 RALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRW 217

Query: 345 RYAPKFENGKAVEAQTSVQMDYTIN 369
RY P V V + + IN
Sbjct: 218 RYEPGKPGSGIV-----VNILFKIN 237


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4051RTXTOXIND561e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 56.0 bits (135), Expect = 1e-10
Identities = 31/220 (14%), Positives = 71/220 (32%), Gaps = 25/220 (11%)

Query: 80 FELAVSHAKLALEQVRQDNAELDASLLAAKAEVNASATTAQQKRREAKRLDALYVTHGVS 139
+ S + Q + + A L A +N ++ ++ +L ++
Sbjct: 191 IKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIA 250

Query: 140 -------QQLRDQADSDAAAAEANLLAANARLEKLKVSRGFYGED------------NLR 180
+ +A ++ ++ L + + K +
Sbjct: 251 KHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDN 310

Query: 181 VRQAMNALAQAELNLSYTQIRADQDGVVTNLQL-EVGSFAAVGQPLLALV--SDKLDIIA 237
+ LA+ E + IRA V L++ G + L+ +V D L++ A
Sbjct: 311 IGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTA 370

Query: 238 DFREKTLRGVNAAYPALIAFDGEPGRLYH---AQVSSVDA 274
+ K + +N A+I + P Y +V +++
Sbjct: 371 LVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINL 410



Score = 54.8 bits (132), Expect = 2e-10
Identities = 31/197 (15%), Positives = 66/197 (33%), Gaps = 18/197 (9%)

Query: 1 MTPDQQFARLVKIAMLGFVAV-FGYFMFADTMMPLTPQAMATRVVT------KVTPQISG 53
TP + RLV ++GF+ + F + + A A +T ++ P +
Sbjct: 50 ETPVSRRPRLVAYFIMGFLVIAFILSVLG----QVEIVATANGKLTHSGRSKEIKPIENS 105

Query: 54 KIQSINVNNNQVVAKGDLLFQVDPAPFELAVSHAKLALEQVRQDNAELDASLLAA-KAEV 112
++ I V + V KGD+L ++ E + +L Q R + + ++
Sbjct: 106 IVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKL 165

Query: 113 NASATTAQQKRREAKRLDALYVTHGVSQQL------RDQADSDAAAAEANLLAANARLEK 166
+ + + L +T + +Q + Q + + A L AR+ +
Sbjct: 166 PELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINR 225

Query: 167 LKVSRGFYGEDNLRVRQ 183
+
Sbjct: 226 YENLSRVEKSRLDDFSS 242


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4814PYOCINKILLER270.013 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 27.1 bits (59), Expect = 0.013
Identities = 10/37 (27%), Positives = 18/37 (48%)

Query: 37 PPLLSPTPENPLFALRLTFTKALRKLSNKLLVNNKKV 73
P + P + + AL++ + KLL+N KK+
Sbjct: 105 GPAKNLAPLDVINRSLTIVGNALQQKNQKLLLNQKKI 141


48SO_4144SO_4150Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_41443347.817823octaheme tetrathionate reductase Otr
SO_41453348.176379signalling protein with EAL and C2 domains
SO_41464358.677463type I secretion system ATPase subunit HlyB
SO_41474379.224512type I secretion system ATPase and inner
SO_414853910.000756type I protein secretion system MFP component
SO_414954110.672745secreted VCBS domain protein
SO_41502153.439588sulphate transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4148RTXTOXIND2748e-90 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 274 bits (703), Expect = 8e-90
Identities = 117/447 (26%), Positives = 209/447 (46%), Gaps = 14/447 (3%)

Query: 8 HRSLPEHEFTQAIEAPAEKRIIKQ---ITYFIAGSVFIMFVWSLFTNIEEIAKAKGQVIP 64
R E+EF A E + ++ + YFI G + I F+ S+ +E +A A G++
Sbjct: 33 VREKDENEFLPAHLELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTH 92

Query: 65 LGHQQVIQSFSGGTLASILVSEGDLVKKGDVLANFIAIDSQAAAEELESKQANLVLKIER 124
G + I+ + I+V EG+ V+KGDVL A+ ++A + +S L+ R
Sbjct: 93 SGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTR 152

Query: 125 YSAFIESREANFASYLESHPNLVKGHISDLERM----------NNEKQAIIQSSLAEIAK 174
Y S E N L+ ++S+ E + + + Q L + K
Sbjct: 153 YQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELN-LDK 211

Query: 175 SKAELASLDQEIPPLKHQITSAQQTINMMESIKESQAVSKLTMLESQQKLDSYIRELKSM 234
+AE ++ I ++ + ++ S+ QA++K +LE + K + EL+
Sbjct: 212 KRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVY 271

Query: 235 EGKQQVLARDIENLQRQLEQKQATLLKEVGEARTDAQAELLGITARLKSSDSQVQQNTIT 294
+ + + + +I + + + + E+ + + +T L ++ + Q + I
Sbjct: 272 KSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIR 331

Query: 295 SPVDGIIQSIPNTSSGSVIQPGGTVAVIVPTTPTALLEAKLSPRDIGFVSVGQKARIKID 354
+PV +Q + + G V+ T+ VIVP T + A + +DIGF++VGQ A IK++
Sbjct: 332 APVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVE 391

Query: 355 AFDYSRYGALDGIVKRISPSTDADEKGGVFYKVQISIDKPYFGDQPDKLELIPGMTGEAD 414
AF Y+RYG L G VK I+ D++ G+ + V ISI++ + L GM A+
Sbjct: 392 AFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAE 451

Query: 415 IVTGDKTVFQYLWKPVFTNVTEAFGER 441
I TG ++V YL P+ +VTE+ ER
Sbjct: 452 IKTGMRSVISYLLSPLEESVTESLRER 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4149MICOLLPTASE370.002 Microbial collagenase metalloprotease (M9) signature.
		>MICOLLPTASE#Microbial collagenase metalloprotease (M9) signature.

Length = 1104

Score = 37.4 bits (86), Expect = 0.002
Identities = 37/197 (18%), Positives = 67/197 (34%), Gaps = 31/197 (15%)

Query: 2116 TFSSAQSVDGFTLNADG---SYSFD-----PSHASYQHLAAGQTQTLTIPVTVTDSEGAT 2167
F +S D DG +Y +D S+ + +T + +TVTD+ G
Sbjct: 792 NFDGTESKD-----EDGEIKAYEWDFGDGEKSNEAKATHKYNKTGEYEVKLTVTDNNGGI 846

Query: 2168 STQNLTIRLTGTNDAPHI------SGADVGRVVEDQTLSVSGKLAISDADDGQAHFIAQT 2221
+T++ I++ I + + + + V G L+ D D +A+
Sbjct: 847 NTESKKIKVVEDKPVEVINESEPNNDFEKANQIAKSNMLVKGTLSEEDYSDKYYFDVAKK 906

Query: 2222 ATTGSYGSLTIGEDGQWQ---------YQLDNTKPEVQALKSTETATDT---FTVHSADG 2269
+ W Y L T + LK +T +V++ D
Sbjct: 907 GNVKITLNNLNSVGITWTLYKEGDLNNYVLYATGNDGTVLKGEKTLEPGRYYLSVYTYDN 966

Query: 2270 SSHNITITIQGQRDNVV 2286
S T+ ++G N V
Sbjct: 967 QSGTYTVNVKGNLKNEV 983


49SO_4207SO_4219Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4207217-0.8111747TM intracellular signalling protein with GGDEF
SO_42083230.706962delta-aminolevulinic acid dehydratase HemB
SO_42114221.057807**preprotein translocase ATPase subunit SecA
SO_42122161.620310peptidase M23 family
SO_42131172.181243protein of unknown function DUF721
SO_42141172.646582UDP-3-0-acyl N-acetylglucosamine deacetylase
SO_42152183.069787cell division filament FtsZ
SO_42161173.068590cell division protein FtsA
SO_42171182.588659cell division protein FtsQ
SO_42180172.793518UDP-N-acetylmuramate--alanine ligase MurC
SO_42190163.203581undecaprenyldiphospho-muramoylpentapeptide
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4207PF02370320.005 M protein repeat
		>PF02370#M protein repeat

Length = 168

Score = 31.6 bits (71), Expect = 0.005
Identities = 13/69 (18%), Positives = 32/69 (46%)

Query: 388 DERKAKQRIQQEALKQAQKIRSAREEALKVEAETNERLEQKVQERTLELEITLRELHEVN 447
D RK + + Q + + ++ + +E + E + ++ QE+ + + ++L
Sbjct: 59 DLRKREGQYQDKIEELEKERKEKQERPERREKFERQHQDKHYQEQQKKHQQEQQQLEAEK 118

Query: 448 QKLTEQSTI 456
QKL ++ I
Sbjct: 119 QKLAKEKQI 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4211SECA13240.0 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 1324 bits (3429), Expect = 0.0
Identities = 660/907 (72%), Positives = 767/907 (84%), Gaps = 7/907 (0%)

Query: 1 MFGKLLTKVFGSRNDRTLKGLQKVVNKINALEADYEKLTDEQLKAKTAEFRERLAAGASL 60
M KLLTKVFGSRNDRTL+ ++KVVN INA+E + EKL+DE+LK KTAEFR RL G L
Sbjct: 1 MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVL 60

Query: 61 DSIMAEAFATVREASKRVFEMRHFDVQLLGGMVLDSNRIAEMRTGEGKTLTATLPAYLNA 120
++++ EAFA VREASKRVF MRHFDVQLLGGMVL+ IAEMRTGEGKTLTATLPAYLNA
Sbjct: 61 ENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNA 120

Query: 121 LTGKGVHVITVNDYLARRDAENNRPLFEFLGLTVGINVAGLGQQDKKDAYNADITYGTNN 180
LTGKGVHV+TVNDYLA+RDAENNRPLFEFLGLTVGIN+ G+ K++AY ADITYGTNN
Sbjct: 121 LTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNN 180

Query: 181 EFGFDYLRDNMAFSPQERVQRPLHYALIDEVDSILIDEARTPLIISGAAEDSSELYIKIN 240
E+GFDYLRDNMAFSP+ERVQR LHYAL+DEVDSILIDEARTPLIISG AEDSSE+Y ++N
Sbjct: 181 EYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVN 240

Query: 241 TLIPNLIRQDKEDSEEYVGEGDYTIDEKAKQVHFTERGQEKVENLLIERGMLAEGDSLYS 300
+IP+LIRQ+KEDSE + GEG +++DEK++QV+ TERG +E LL++ G++ EG+SLYS
Sbjct: 241 KIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYS 300

Query: 301 AANISLLHHVNAALRAHTLFERDVDYIVQDGEVIIVDEHTGRTMPGRRWSEGLHQAVEAK 360
ANI L+HHV AALRAH LF RDVDYIV+DGEVIIVDEHTGRTM GRRWS+GLHQAVEAK
Sbjct: 301 PANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAK 360

Query: 361 EGVRIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFQHIYGLDTVVVPTNRPMVR 420
EGV+IQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEF IY LDTVVVPTNRPM+R
Sbjct: 361 EGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIR 420

Query: 421 KDMADLVYLTANEKYQAIIKDIKDCRERGQPVLVGTVSIEQSELLARLMVKEKIPHQVLN 480
KD+ DLVY+T EK QAII+DIK+ +GQPVLVGT+SIE+SEL++ + K I H VLN
Sbjct: 421 KDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLN 480

Query: 481 AKFHEKEAEIVAQAGRTGAVTIATNMAGRGTDIVLGGNWNMEIDALENPTPEQKAKIKAD 540
AKFH EA IVAQAG AVTIATNMAGRGTDIVLGG+W E+ ALENPT EQ KIKAD
Sbjct: 481 AKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKAD 540

Query: 541 WQLRHDAVVAAGGLHILGTERHESRRIDNQLRGRAGRQGDAGSSRFYLSMEDSLMRIFAS 600
WQ+RHDAV+ AGGLHI+GTERHESRRIDNQLRGR+GRQGDAGSSRFYLSMED+LMRIFAS
Sbjct: 541 WQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFAS 600

Query: 601 DRVSGMMKKLGMEEGEAIEHPWVSRAIENAQRKVEARNFDIRKQLLEFDDVANDQRQVVY 660
DRVSGMM+KLGM+ GEAIEHPWV++AI NAQRKVE+RNFDIRKQLLE+DDVANDQR+ +Y
Sbjct: 601 DRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIY 660

Query: 661 AQRNELMDAESIADTIQNIQDDVISAVIDQYIPPQSVEELWDVPGLEQRLHQEFMLKLPI 720
+QRNEL+D +++TI +I++DV A ID YIPPQS+EE+WD+PGL++RL +F L LPI
Sbjct: 661 SQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPI 720

Query: 721 QEWLDKEDDLHEESLRERIITSWSDAYKAKEEMVGASVLRQFEKAVMLQTLDGLWKEHLA 780
EWLDKE +LHEE+LRERI+ + Y+ KEE+VGA ++R FEK VMLQTLD LWKEHLA
Sbjct: 721 AEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLA 780

Query: 781 AMDHLRQGIHLRGYAQKNPKQEYKRESFELFQQLLNTLKHDVISVLSKVQVQAQSDVEEM 840
AMD+LRQGIHLRGYAQK+PKQEYKRESF +F +L +LK++VIS LSKVQV+ +VEE+
Sbjct: 781 AMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEEL 840

Query: 841 EARRREEDAKIQRDYQHAAAEALVGGSDEDDAIAAHTPMIRDGDKVGRNDPCPCGSGRKY 900
E +RR E A + D+D A AA KVGRNDPCPCGSG+KY
Sbjct: 841 EQQRRMEAE-------RLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKY 893

Query: 901 KQCHGKL 907
KQCHG+L
Sbjct: 894 KQCHGRL 900


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4216SHAPEPROTEIN696e-15 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 68.6 bits (168), Expect = 6e-15
Identities = 51/221 (23%), Positives = 91/221 (41%), Gaps = 20/221 (9%)

Query: 150 SGMRMEAKVHIVTC----ANDMAKNITK-SVERCGLKVDDLVFSGIASADAVLTFDEKDL 204
S M ++ C A + + + S + G + L+ +A+A +
Sbjct: 100 SNSFMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEAT 159

Query: 205 GVCIVDIGGGTTDIAVYTNGALRHCAVVPVAGNQVTNDIAKIFR------TPSSHAEQIK 258
G +VDIGGGTT++AV + + + + V + G++ I R + AE+IK
Sbjct: 160 GSMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIK 219

Query: 259 VQFACARSSMVSREDSIEVPS---VGGRPSR-SMSRHTLAEVVEPRYQELFELVLKELKD 314
+ A IEV G P +++ + + E ++ + V+ L+
Sbjct: 220 HEIGSAYPG--DEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQ 277

Query: 315 SGLE---DQIAAGIVLTGGTASIQGVVDIAEATFGMPVRVA 352
E D G+VLTGG A ++ + + G+PV VA
Sbjct: 278 CPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVA 318


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4219LIPPROTEIN48290.033 Mycoplasma P48 major surface lipoprotein signature.
		>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature.

Length = 428

Score = 28.8 bits (64), Expect = 0.033
Identities = 24/125 (19%), Positives = 42/125 (33%), Gaps = 25/125 (20%)

Query: 111 GVAAKLAGVPLVLHEQNAIPGMTNKLLSRIASQVLCAFKNTFTQVKAKVVGNPIRRELIA 170
G++ A +P V G ++ + + + T K V
Sbjct: 10 GLSPIAAILPAVA----VSCGNNDESNISFKEKDISKYTTTNANGKQVV----------- 54

Query: 171 LGGEPKQTADEALKVLVV--GGSLGAKVFNDLMPEVVAALSKQQSITVWHQVGKDNLAGV 228
K LK +++ G + K FN E + A++KQ I + + N
Sbjct: 55 -----KNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINNVEPSSNF--- 106

Query: 229 KSAYQ 233
+SAY
Sbjct: 107 ESAYN 111


50SO_4257SO_4289Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4257017-3.899832protein of unknown function TIGR00255
SO_4259014-3.205098ISSod4 transposase TnpA_ISSod4
SO_4261013-2.500847hypothetical protein
SO_4262-111-1.716062phage P1-related protein in restrction
SO_4263-111-1.598081phage P1-related protein in restrction
SO_4264-110-1.458044type I restriction-modification system
SO_4265-110-2.048746type I restriction-modification system
SO_4266-112-3.047203Fic family protein MloA
SO_4267-113-3.409573type I restriction-modification system
SO_4268-223-4.781958ISSod2 transposase TnpA_ISSod2
SO_4270023-4.941622hypothetical protein
SO_4274124-4.417506undecaprenol diphosphatase UppP
SO_4825221-3.303621redox-active disulfide protein
SO_4278023-5.190908ISSod1 transposase TnpA_ISSod1
SO_4279230-7.344054tellurium ion resistance family protein
SO_4280026-6.478269CBS domain containing protein
SO_4844029-7.615861hypothetical protein
SO_4281030-8.160223sodium-dependent potassium uptake system NAD
SO_4282129-7.907680sodium-dependent potassium uptake system
SO_4283124-6.879067ApbE family lipoprotein
SO_4284022-5.547306ISSod5 transposase TnpA_ISSod5
SO_4286130-6.939543stator-force generator of H+ coupled flagellar
SO_4287129-6.083648stator-force generator of H+ coupled flagellar
SO_4288023-4.909958exopolysaccharide synthesis protein
SO_4289-119-3.619029ABC-type phosphate uptake system permease ATPase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4286OMPADOMAIN587e-12 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 58.0 bits (140), Expect = 7e-12
Identities = 28/121 (23%), Positives = 55/121 (45%), Gaps = 16/121 (13%)

Query: 154 NNTFFASGSAFIQPKFIPLIDKIGEVIASV---PGRVVIAGHTDATLPMEIYADNWDLSS 210
++ F A ++P+ +D++ ++++ G VV+ G+TD N LS
Sbjct: 219 SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAY---NQGLSE 275

Query: 211 LRATAVVRIMTKNKGVNPSRIIVQGLADTQPRFQNDTPEHRQK---------NRRIEIIL 261
RA +VV + KG+ +I +G+ ++ P N +Q+ +RR+EI +
Sbjct: 276 RRAQSVVDYLIS-KGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 262 N 262

Sbjct: 335 K 335


51SO_4304SO_4317Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_43040193.319791nickel uptake transporter HupE
SO_43050192.939363HAD-superfamily hydrolase subfamily IA variant 1
SO_43060182.824821tyrosine recombinase XerC
SO_4307-2181.155441protein of unknown function DUF484
SO_4308-2141.710338diaminopimelate epimerase DapF
SO_4309-2131.477762diaminopimelate decarboxylase LysA
SO_4310-1120.624374putative lipoprotein
SO_43110110.406878iron donor for FeS cluster assembly CyaY
SO_43122152.093657adenylate cyclase CyaA
SO_43132172.024127hydroxymethylbilane synthase HemC
SO_43142161.521034uroporphyrinogen-III synthase HemD
SO_43152161.218588uroporphyrin-III C-methyltransferase HemX
SO_43163161.086335protoheme synthesis protein HemY
SO_43173171.015736biofilm-promoting protein BpfA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4317CABNDNGRPT752e-15 NodO calcium binding signature.
		>CABNDNGRPT#NodO calcium binding signature.

Length = 479

Score = 74.6 bits (183), Expect = 2e-15
Identities = 40/124 (32%), Positives = 55/124 (44%), Gaps = 9/124 (7%)

Query: 2580 DTVNLGSGDDTVNGGQGSQLVYGGSGDDLLIGGEGIDGLRGGDGNDTLIGGLGDDVLRGD 2639
++ G + GG G+ ++ G S D++L GG G D L GG G DTL GG G D
Sbjct: 332 VSIAHGVTIENAIGGSGNDILVGNSADNILQGGAGNDVLYGGAGADTLYGGAGRDTFVYG 391

Query: 2640 SGADTFVWRYADADKGTDHIMDFKVGEDKLDLSDLLQGETANTLESYLKFSLNNGSTVID 2699
SG D+ V D I DF+ G DK+DLS +F+ ++
Sbjct: 392 SGQDSTV-------AAYDWIADFQKGIDKIDLSAF--RNEGQLSFVQDQFTGKGQEVMLQ 442

Query: 2700 IDAN 2703
DA
Sbjct: 443 WDAA 446


52SO_4340SO_4399Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_43401133.364715putative transport protein with Tim44-like
SO_43410143.508377hypothetical protein
SO_43421195.977585predicted lipoprotein
SO_43431196.037681serine-pyruvate aminotransferase AgxT
SO_43440205.337229threonine dehydratase IlvA
SO_43451185.053519dihydroxy-acid dehydratase IlvD
SO_43461152.301349acetolactate synthase II small subunit IlvM
SO_43470152.164269acetolactate synthase II large subunit IlvG
SO_4348-110-1.471216ilvGM operon attenuation leader peptide IlvL
SO_4349-111-2.540436ketol-acid reductoisomerase IlvC
SO_4350016-3.587341acetohydroxybutyrate/acetolactate-responsive
SO_4351121-4.499242hemolysin HlyA
SO_4354124-5.965333protein of unknown function UPF0153
SO_4355225-6.251415cAMP-binding regulator
SO_4356226-5.747588predicted lipoprotein
SO_4357326-6.491842extracellular oxidoreductase FeS binding
SO_4358219-4.175311extracellular oxidoreductase
SO_4359116-3.878422outer membrane protein MtrB family
SO_4360015-1.221978periplasmic decaheme cytochrome c MtrA family
SO_4361-1140.491751extracellular oxidoreductase associated protein
SO_4362-2161.240406extracellular oxidoreductase chaperone protein
SO_4364-1172.738896ATP-dependent DNA helicase RecG
SO_4365-2181.395157protein of unknown function DUF3014
SO_43660222.419437hypothetical protein
SO_4367-1252.525216acyltransferase family protein
SO_4368-2253.482136acyl carrier protein
SO_4369-1234.414609acyl carrier protein
SO_4370-1244.486262membrane protein
SO_43710234.499147AMP-dependent synthetase and ligase family
SO_43721234.472205thioester dehydrase family protein
SO_43730224.558048glycosyl transferase family 2
SO_43741214.052482phenylalanine/tyrosine ammonia-lyase
SO_43752234.132506acyl-CoA thioester hydrolase, YbgC/YbaW family
SO_43762234.283081hypothetical protein
SO_43772234.606800MMPL family efflux pump permease component
SO_43781215.378907FAD-binding protein
SO_43792204.005898fatty acid biosynthesis locus lipoprotein of
SO_43802171.620711beta-ketoacyl synthase
SO_4381114-0.533663thioester dehydrase family protein
SO_4382014-1.5127463-oxoacyl-(acyl-carrier-protein) reductase FabG
SO_4383014-3.4650673-oxoacyl-(acyl-carrier-protein) synthase II
SO_4384015-5.280170hypothetical protein
SO_4385-118-5.114865von Willebrand factor type A domain protein
SO_4386019-3.876929ISSod4 transposase TnpA_ISSod4
SO_4388-121-3.037171two component signal transduction system
SO_4391-118-1.477682protein of unknown function DUF2971
SO_4393-1181.858725acteyltransferase GNAT family YsnE
SO_43940161.958814cytoplasmic rhodanese domain protein
SO_4395-1162.133052hypothetical protein
SO_43961182.077281FMN-dependent NADH-azoreductase AzoR
SO_43972212.358895thioesterase domain protein YiiD
SO_43981192.157419D-tyrosyl-tRNA(Tyr) deacylase Dtd
SO_43992212.686715hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4340TCRTETB290.017 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.5 bits (66), Expect = 0.017
Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 6/83 (7%)

Query: 67 MMGGMLGGLLAGGLLAALFMGEGFENIQFMDILIIALLAFVLFKIVRTVMASKASAQPRP 126
+ G+ L+ L A F+ E FM I+I+ +L + F +TV+++ S+ +
Sbjct: 326 LNIGVT--FLSVSFLTASFLLET--TSWFMTIIIVFVLGGLSF--TKTVISTIVSSSLKQ 379

Query: 127 AYAGAGQPNPNLQRQQAEQTGFA 149
AGAG N +E TG A
Sbjct: 380 QEAGAGMSLLNFTSFLSEGTGIA 402


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4357PF03627280.037 PapG
		>PF03627#PapG

Length = 336

Score = 27.6 bits (61), Expect = 0.037
Identities = 9/26 (34%), Positives = 15/26 (57%)

Query: 41 TYEYCGGNWTADGQGAYHQDVFAYYI 66
T+ C G ADG AY+++ A+ +
Sbjct: 60 TWNQCNGPGFADGSWAYYREYIAWVV 85


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4359PF00577310.016 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 31.0 bits (70), Expect = 0.016
Identities = 11/64 (17%), Positives = 29/64 (45%), Gaps = 3/64 (4%)

Query: 567 DYRYSESDSQSRIQPRASADY---FNFTHQFDISLNYELSKSDSLSLSYRYERYFDTDAA 623
Y D +++P+ + Y +N + +++ +L ++ +L LS ++ Y+ T
Sbjct: 499 GYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNV 558

Query: 624 NVDI 627
+
Sbjct: 559 DEQF 562


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4364SECA403e-05 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 40.2 bits (94), Expect = 3e-05
Identities = 31/84 (36%), Positives = 39/84 (46%), Gaps = 8/84 (9%)

Query: 289 MRLVQGDV-----GSGKTLVAALAA-LQAIENGYQVAMMAPTELLAEQHAANFAAWFEPL 342
M L + + G GKTL A L A L A+ G V ++ + LA++ A N FE L
Sbjct: 92 MVLNERCIAEMRTGEGKTLTATLPAYLNALT-GKGVHVVTVNDYLAQRDAENNRPLFEFL 150

Query: 343 GLKVGW-LAGKLKGKARAQSLADI 365
GL VG L G R ADI
Sbjct: 151 GLTVGINLPGMPAPAKREAYAADI 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4369FRAGILYSIN250.033 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 25.0 bits (54), Expect = 0.033
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 4/74 (5%)

Query: 1 MQNREQILAMLTTILVDEFEIDADAITP--DANLYEELDLDSIDAVDLVIKLQQL--TGK 56
M+N + +L + T L+ +AD++T DA + +DL S+ DL +L + GK
Sbjct: 9 MKNVKLLLMLGTAALLAACSNEADSLTTSIDAPVTASIDLQSVSYTDLATQLNDVSDFGK 68

Query: 57 KIQPDEFKSVRTVN 70
I + R V+
Sbjct: 69 MIILKDNGFNRQVH 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4377ACRIFLAVINRP367e-04 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 36.0 bits (83), Expect = 7e-04
Identities = 26/147 (17%), Positives = 52/147 (35%), Gaps = 21/147 (14%)

Query: 689 LLGLALVIALLLFSLSFGLKKATVVVAVPALAAVLTLAILGLVGSPLSLFHALALILIFG 748
+ LV ++ L +AVP + + T AIL G ++ ++L G
Sbjct: 345 FEAIMLVFLVMYLFLQNMRATLIPTIAVP-VVLLGTFAILAAFGYSINTLTMFGMVLAIG 403

Query: 749 IGIDYSL----------------FFASVEQHGKAVMMAVFMSACSTLLAFGLLAFSQTQA 792
+ +D ++ + E+ + A+ A F +AF
Sbjct: 404 LLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGST 463

Query: 793 ---IHYFGLTLSLGIGFTFVLSPLILT 816
F +T+ + + +++ LILT
Sbjct: 464 GAIYRQFSITIVSAMALSVLVA-LILT 489



Score = 34.4 bits (79), Expect = 0.002
Identities = 35/195 (17%), Positives = 72/195 (36%), Gaps = 18/195 (9%)

Query: 276 LGLASLLGVVLLVWLAFRSVMPLLLAIVTISSGLLLAVTFTLSVFGELHLLTLVFGTSLI 335
L A +L V L+++L +++ L+ + + LL + ++ LT+ I
Sbjct: 344 LFEAIML-VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAI 402

Query: 336 GIAIDYSFHFY--CERLNEQHHSAQATVAYI------FPTVSLAFITSALAYVGIGLAPF 387
G+ +D + ER+ + V +A + SA V I +A F
Sbjct: 403 GLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSA---VFIPMAFF 459

Query: 388 PG-----MQQVAIFCASGLLGAYLTLVLAYPLLAGSKL-PSGEQPLNLAQAYLARMAQFS 441
G +Q +I S + + L ++ P L + L P + +
Sbjct: 460 GGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTF 519

Query: 442 NKLVSPWGLSLFTLI 456
+ V+ + S+ ++
Sbjct: 520 DHSVNHYTNSVGKIL 534



Score = 34.0 bits (78), Expect = 0.003
Identities = 23/117 (19%), Positives = 43/117 (36%), Gaps = 17/117 (14%)

Query: 689 LLGLA-LVIALLLFSLSFGLKKATVVVAVPALAAVLTLAILGLVGSPLSLFHALALILIF 747
L+ ++ +V+ L L +L V+ V L V L L ++ + L+
Sbjct: 875 LVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTI 934

Query: 748 GIGIDYSLFFASV-----EQHGKAVMMAV-----------FMSACSTLLAFGLLAFS 788
G+ ++ E+ GK V+ A M++ + +L LA S
Sbjct: 935 GLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAIS 991


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4382DHBDHDRGNASE1073e-30 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 107 bits (267), Expect = 3e-30
Identities = 71/248 (28%), Positives = 114/248 (45%), Gaps = 15/248 (6%)

Query: 5 VLVTGSSRGIGKAIALKLAAAGHDIALHYHSNQAAADASAAELRALGVNVSLLKFDVADR 64
+TG+++GIG+A+A LA+ G IA N + + L+A + DV D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIA-AVDYNPEKLEKVVSSLKAEARHAEAFPADVRDS 69

Query: 65 VAVRAALEADIEANGAYYGVVLNAGINRDNAFPAMSEAEWDSVIHTNLDGFYNVIHPCVM 124
A+ G +V AG+ R ++S+ EW++ N G +N V
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS-VS 128

Query: 125 PMVQARKGGRIITLASVSGIAGNRGQVNYSASKAGLIGATKALSLELAKRKITVNCIAPG 184
+ R+ G I+T+ S Y++SKA + TK L LELA+ I N ++PG
Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188

Query: 185 LIETDM----------VADIPKDMVEQL---VPMRRMGKPNEIAALAAFLMSDDAAYITR 231
ETDM + K +E +P++++ KP++IA FL+S A +IT
Sbjct: 189 STETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITM 248

Query: 232 QVISVNGG 239
+ V+GG
Sbjct: 249 HNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4388HTHFIS1022e-27 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 102 bits (255), Expect = 2e-27
Identities = 38/128 (29%), Positives = 60/128 (46%), Gaps = 4/128 (3%)

Query: 2 KILIAEDDIHIRQGLADMLSREGYSVLLADNGKVALLKYQQEQPDFIILDIMMPELDGYS 61
IL+A+DD IR L LSR GY V + N D ++ D++MP+ + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VCKEIRKHDEQTPVIFLSAKGEELDKVLGLELGADDYINKPFGIHEVRARIKTIARRCLK 121
+ I+K PV+ +SA+ + + E GA DY+ KPF + E+ I R L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGII----GRALA 120

Query: 122 AKQNSPDQ 129
+ P +
Sbjct: 121 EPKRRPSK 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4393SACTRNSFRASE386e-06 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 37.6 bits (87), Expect = 6e-06
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 2/80 (2%)

Query: 61 DRNLAGCGALKWLDAEHAEIKSMRTAATYKQQGVASQILQHLINDAKAAGVQRLSLETGS 120
+ N G ++ +A I+ + A Y+++GV + +L I AK L LET
Sbjct: 73 ENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQD 132

Query: 121 MTFFQPARSLYAKFGFELCG 140
+ A YAK F +
Sbjct: 133 INI--SACHFYAKHHFIIGA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4396cdtoxinb280.034 Cytolethal distending toxin B signature.
		>cdtoxinb#Cytolethal distending toxin B signature.

Length = 269

Score = 27.7 bits (61), Expect = 0.034
Identities = 16/53 (30%), Positives = 25/53 (47%), Gaps = 1/53 (1%)

Query: 1 MSKVLVLKSSILGGYSQSALLVDYLIGKWEKQGATITVRDLAGKDVLPMVDGE 53
M K ++ L Y+Q A L D+ + W QGA+ T +V ++ GE
Sbjct: 1 MKKYIISLIVFLSFYAQ-ADLTDFRVATWNLQGASATTESKWNINVRQLISGE 52


53SO_4583SO_4591Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_45832110.790453RNA polymerase sigma-32 factor RpoH
SO_45842101.053726ABC-type cell division-associated export system
SO_4585290.788787ABC-type cell division-associated export system
SO_45864100.062858signal recognition particle docking protein
SO_4587114-1.24999316S rRNA (guanine966-N2)-methyltransferase RsmD
SO_4588216-2.163847membrane protein of unknown function DUF1145
SO_4589216-1.258240transcriptional regulator AraC family
SO_4590416-1.418778isochorismatase family protein
SO_4591216-0.731030membrane anchored tetraheme cytochrome c CymA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4584PF07201280.040 Hypersensitivity response secretion protein HrpJ
		>PF07201#Hypersensitivity response secretion protein HrpJ

Length = 293

Score = 28.3 bits (63), Expect = 0.040
Identities = 19/92 (20%), Positives = 37/92 (40%), Gaps = 6/92 (6%)

Query: 50 LGVSLSLPAA-LQVLVKNAETITSSWNSAAEISL-----FIDENRSEQTIQSLLTRIRTY 103
G S+ + + LQ + AE +T ++ E+SL + R + + +
Sbjct: 35 RGESVQIVSGTLQSIADMAEEVTFVFSERKELSLDKRKLSDSQARVSDVEEQVNQYLSKV 94

Query: 104 PEVEKVQYIDRNQALEEFQRLSGFGEALAYLD 135
PE+E+ Q + +L + AYL+
Sbjct: 95 PELEQKQNVSELLSLLSNSPNISLSQLKAYLE 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4586IGASERPTASE588e-11 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 57.8 bits (139), Expect = 8e-11
Identities = 38/198 (19%), Positives = 70/198 (35%), Gaps = 14/198 (7%)

Query: 17 DEVVEQTPV-VTPS-QTEQDEALAEQQAEAAR---------LAAEKIAAENAEAERIAAE 65
DE P TPS TE ++Q+++ A + A+ A++ A
Sbjct: 1021 DEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANT 1080

Query: 66 QATKAQVEALRLAEAEEQAIKAQAAAERFVEQQAAETARLAAEQALAAQLAAEKAESERI 125
Q + E + K A E+ E+ ET + + +Q++ ++ +SE +
Sbjct: 1081 QTNEVAQSGSETKETQTTETKETATVEK-EEKAKVETEKTQEVPKVTSQVSPKQEQSETV 1139

Query: 126 ATEQAAKAQAEVEALRLAEEQAEVARLAEQ-QAAEATRLAAEQALAEQLAAEKAEAEQIQ 184
QA A+ + + E Q++ A+ Q A+ T EQ + E +
Sbjct: 1140 -QPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVEN 1198

Query: 185 VEQPLEQQPEPQAKPAKE 202
E +P
Sbjct: 1199 PENTTPATTQPTVNSESS 1216



Score = 53.9 bits (129), Expect = 1e-09
Identities = 33/194 (17%), Positives = 71/194 (36%), Gaps = 40/194 (20%)

Query: 17 DEVVEQTPVVTPSQTEQDEALAEQQAEAARLAAEKI-------AAENAEAERIAAEQATK 69
++ V+ T + TP+ + D + ++ A + E +A +
Sbjct: 989 NQTVDTTNITTPNNIQADVP-SVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQE 1047

Query: 70 AQVEALRLAEAEEQAIKAQAAAERFVEQQAAETARLAAEQALAAQLAAEKAESERIATEQ 129
++ +A E + + A+ +A + + AQ +E E++ T++
Sbjct: 1048 SKTVEKNEQDATETTAQNREVAK-----EAKSNVKANTQTNEVAQSGSETKETQTTETKE 1102

Query: 130 AAKAQAEVEALRLAEEQAEVARLAEQQAAEATRLAAEQALAEQLAAEKAEAEQIQVEQPL 189
A + E +A E+ EV ++ Q + + E++E Q
Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQ---------------EQSETVQ------- 1140

Query: 190 EQQPEPQAKPAKES 203
PQA+PA+E+
Sbjct: 1141 -----PQAEPAREN 1149


54SO_4603SO_4643Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_46032141.809237bifunctional transcriptional repressor of
SO_46043121.553199UV induced cell division inhibitor SulA
SO_46062110.994744aa3-type cytochrome c oxidase subunit II CoxB
SO_46072130.459327aa3 type cytochrome c oxidase subunit I CoxA
SO_4608214-0.013820aa3 type cytochrome c oxidase assembly protein
SO_46092130.354587aa3 type cytochrome c oxidase subunit III CoxC
SO_46102150.061322hypothetical protein
SO_46110140.684651aa3 type cytochrome c oxidase biogenesis protein
SO_46120151.432313hypothetical protein
SO_4613-1140.893876heme A synthase CtaA
SO_4614-1120.589084protoheme IX farnesyltransferase (heme O
SO_4615-19-0.319814cytochrome c oxidase biogenesis protein SenC
SO_4616-19-1.466874carbohydrate esterase family 4
SO_4617-110-2.471706DNA damage-inducible multidrug and toxin efflux
SO_4618-212-3.029777subfamily S9C unassigned peptidase
SO_4619-219-4.112730iron-sulfur cluster biogenesis scaffold protein
SO_4620-119-3.404930FAD-dependent oxidoreductase
SO_4621017-2.752987nucleoside-specifc outer membrane porin Tsx
SO_4622116-0.366175two component signal transduction system
SO_4623-2131.218630two component signal transduction system
SO_4624-2152.010092transcriptional regulator LuxR family
SO_4625-2152.751372predicted phosphoribosyltransferase ComF family
SO_4626-1153.113880biotin biosynthesis carboxylesterase BioH
SO_4627-1153.220553glutamine rich protein
SO_4628-1153.073031membrane sulfatase HI1246 family
SO_4629-1163.170828transcriptional accessory protein Tex
SO_46310182.564899transcription elongation factor GreB
SO_46330192.041139osmolarity-responsive two component signal
SO_4634-1191.358879osmolarity-responsive two component signal
SO_46350160.835701chemotaxis signal transduction system methyl
SO_4636-114-0.450373predicted lipoprotein
SO_4637116-1.194497two component transcriptional regulator Winged
SO_4638114-1.546082two component signal transduction system
SO_4639420-1.307417periplasmic OB fold (BOF) protein
SO_4640218-0.294043antioxidant AhpC/Tsa family
SO_4641420-0.049173toxin-antitoxin system toxin RelE family
SO_46424170.317898toxin-antitoxin system antidote Phd family
SO_46433151.039137hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4623HTHFIS756e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.3 bits (185), Expect = 6e-18
Identities = 29/124 (23%), Positives = 61/124 (49%), Gaps = 1/124 (0%)

Query: 3 VLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQAEMLISQQQFDAIILDLNLPDGDGIEA 62
+L+ +D+ + + Q L +G + N A I+ D ++ D+ +PD + +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 63 CERWKAQCFSSPIIMLTARSSLNERLAGFAVGADDYLIKPFAMEELVARL-KVVAQRRPA 121
R K P+++++A+++ + GA DYL KPF + EL+ + + +A+ +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 122 PQRL 125
P +L
Sbjct: 126 PSKL 129


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_462756KDTSANTIGN335e-04 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 33.0 bits (75), Expect = 5e-04
Identities = 15/34 (44%), Positives = 17/34 (50%)

Query: 73 QQKQQQQQSSQQQSQQQQEKHAPAVAAERALPKN 106
Q QQQQ QQQ Q + A A AA R L +
Sbjct: 338 PQAQQQQGQGQQQQAQATAQEAVAAAAVRLLNGS 371



Score = 28.0 bits (62), Expect = 0.021
Identities = 10/33 (30%), Positives = 14/33 (42%)

Query: 70 QQVQQKQQQQQSSQQQSQQQQEKHAPAVAAERA 102
Q+ +QQQ Q Q++ A A A E
Sbjct: 328 NQIHLNFVMPPQAQQQQGQGQQQQAQATAQEAV 360


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4633HTHFIS1019e-27 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 101 bits (252), Expect = 9e-27
Identities = 46/175 (26%), Positives = 84/175 (48%), Gaps = 4/175 (2%)

Query: 6 SKILVVDDDMRLRALLERYLMEQGYQVRSAANAEQMDRLLERENFHLIVLDLMLPGEDGL 65
+ ILV DDD +R +L + L GY VR +NA + R + + L+V D+++P E+
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 SICRRLRQQGSTIPIVMLTAKGDEVDRIIGLELGADDYLPKPFNPRELLARI-KAVMRRQ 124
+ R+++ +P+++++A+ + I E GA DYLPKPF+ EL+ I +A+ +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 125 TQDVPGAPAQQEAEIRFGEFSLDLATREMYH---GDEAIVLTSGEFAVLKVLVTH 176
+ Q+ G + + + ++ +GE K LV
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVAR 178


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4634PF06580461e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 46.0 bits (109), Expect = 1e-07
Identities = 19/120 (15%), Positives = 40/120 (33%), Gaps = 24/120 (20%)

Query: 325 DCPEALFQGLAIKRVLSNLVENAFRYG------SGWVRISSQFDGKRIGFSVEDNGPGID 378
A+ ++ LVEN ++G G + + D + VE+ G
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLAL 304

Query: 379 ESQIPTLFQPFTQGDIARGSVGSGLGLA-IIKRIIDRHQGQITLS-NRAAGGLKAQVWLP 436
++ +G GL + +R+ + + + + G + A V +P
Sbjct: 305 KNTKE----------------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4637HTHFIS772e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.8 bits (189), Expect = 2e-18
Identities = 30/168 (17%), Positives = 68/168 (40%), Gaps = 13/168 (7%)

Query: 2 KVLIVDDNHDVIETIMDYLTLEGIIADCAYHGESAINLIQQNHYDVIIMDIMMPKLDGIR 61
+L+ DD+ + + L+ G + + I D+++ D++MP +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 TVKKLREELFCNTPILFLTARDSLQDKIDSFTSGGDDFLNKPF------AMEELCLRLRS 115
+ ++++ + P+L ++A+++ I + G D+L KPF + L
Sbjct: 65 LLPRIKKAR-PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 116 LHNRGPRLDVGILRFGEISLNVATQQACRAGKEIKLSKIQRTILTLLL 163
D + + A Q+ R L+++ +T LTL++
Sbjct: 124 R-RPSKLEDDSQDGMPLVGRSAAMQEIYR-----VLARLMQTDLTLMI 165


55SO_0165SO_0172N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_01651171.163221T2aSS secretion system protein GspC
SO_01661181.540824T2aSS secretion system secretin GspD
SO_01671171.900969T2aSS secretion system assembly ATPase GspE
SO_0168-1161.036462T2aSS secretion system inner membrane platform
SO_0169-1140.881596T2aSS secretion system pseudopilus protein GspG
SO_0170-1151.610722T2aSS secretion system pseudopilus protein GspH
SO_0171-2161.789021T2aSS secretion system pseudopilus protein GspI
SO_0172-1151.471457T2aSS secretion system protein GspJ
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0165BCTERIALGSPC1794e-57 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 179 bits (456), Expect = 4e-57
Identities = 69/286 (24%), Positives = 132/286 (46%), Gaps = 33/286 (11%)

Query: 17 KPLSQVVFWCGFILSLLLAAQITWKLVPTSSSPTAWSPSAVTTTGKGAGQIDLDGLQQLA 76
+ +++F+ +L A I W++ ++P S+V T A Q + L
Sbjct: 12 SVIRRILFYLLMLLFCQQLAMIFWRIGLPDNAPV----SSVQITPAQARQQPV-TLNDFT 66

Query: 77 LFGRADAKTDKPKAEVVETVTDAPKTSLSIQLTGVVASTADQKGLAIIESSGSQETYSLG 136
LFG + K + +++ P ++L++ LTGV+A D + +AII Q + +
Sbjct: 67 LFGVSPEKNKAGALDA-SQMSNLPPSTLNLSLTGVMAGDDDSRSIAIISKDNEQFSRGVN 125

Query: 137 DKIKGTSASLKEVYADRIIITNAGRYETLMLDGLVYTSQSPANQQLQKAKSGKPEVVSRV 196
+++ G +A + + DR+++ GRYE L L +
Sbjct: 126 EEVPGYNAKIVSIRPDRVVLQYQGRYEVLGLYSQEDSGSDGVPG---------------- 169

Query: 197 DQRKNAEISQELAESRTELLADPSKITDYIAISPVKQGESVVGYRLNPGKDVNLFKQAGF 256
A+++++L + + ++DY++ SP+ + GYRLNPG + F + G
Sbjct: 170 -----AQVNEQLQQR------ASTTMSDYVSFSPIMNDNKLQGYRLNPGPKSDSFYRVGL 218

Query: 257 KPNDLAKTINGYDLTVMSQALEMMSQLPELTEVSIMVEREGQLVEI 302
+ ND+A +NG DL QA + M ++ ++ ++ VER+GQ +I
Sbjct: 219 QDNDMAVALNGLDLRDAEQAKKAMERMADVHNFTLTVERDGQRQDI 264


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0166BCTERIALGSPD5930.0 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 593 bits (1529), Expect = 0.0
Identities = 325/681 (47%), Positives = 448/681 (65%), Gaps = 33/681 (4%)

Query: 6 IRRKLIAGVVAGATMLTSQFVWSEQYAANFKGTDIQEFINIVGKNLNKTIIVDPTIRGKI 65
IR + ++ A + +E+++A+FKGTDIQEFIN V KNLNKT+I+DP++RG I
Sbjct: 7 IRSFSLTLLIFAALLFRP--AAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTI 64

Query: 66 NVRSYDLLNDEQYYQFFLNVLQVYGYAIVEMENNVIKVIKDKDAKTAAIRVANDNDPGLG 125
VRSYD+LN+EQYYQFFL+VL VYG+A++ M N V+KV++ KDAKTAA+ VA+D PG+G
Sbjct: 65 TVRSYDMLNEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIG 124

Query: 126 DEMVTRIVALYNTEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLSGRAAVVNKLVEIIR 185
DE+VTR+V L N A+ LAPLLRQLNDNAG G+VV+Y+PSNVL+++GRAAV+ +L+ I+
Sbjct: 125 DEVVTRVVPLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVE 184

Query: 186 RVDKQGDTSVQVVPLEYASAGEMVRIIDTLYRATANQSQLPGQAPKVVADERINAVVVSG 245
RVD GD SV VPL +ASA ++V+++ L + T+ + VVADER NAV+VSG
Sbjct: 185 RVDNAGDRSVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSG 244

Query: 246 DEKSRQRVVELIHRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLESEKDPSAQAA 305
+ SRQR++ +I +LD +QA+ GNTKV YL+YAKA DLVEVLTG + ++SEK A
Sbjct: 245 EPNSRQRIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEK---QAAK 301

Query: 306 GGGKRRNEINIMAHTDTNALVISAEPDQMRTIESVINQLDIRRAQVLVEAIIVEVAEGDN 365
I I AH TNAL+++A PD M +E VI QLDIRR QVLVEAII EV + D
Sbjct: 302 PVAALDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADG 361

Query: 366 VGFGVQWAAKAGGGTQFNNLGPTIGEIGAGIWQAQDKEGTYITNPSTGEVIGQNPKTKGD 425
+ G+QWA K G TQF N G I AG Q +K+GT ++
Sbjct: 362 LNLGIQWANKNAGMTQFTNSGLPISTAIAGANQ-YNKDGTVSSS---------------- 404

Query: 426 VTLLAQALGKVNGMAWGVAMGDFGALVQAVSADTNSNVLATPSITTLDNQEASFIVGDEV 485
LA AL NG+A G G++ L+ A+S+ T +++LATPSI TLDN EA+F VG EV
Sbjct: 405 ---LASALSSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEV 461

Query: 486 PILTGSTASSNNSNPFQTVERKEVGVKLKVVPQINEGNAVKLAIEQEVSGVNG-----NT 540
P+LTGS +++ N F TVERK VG+KLKV PQINEG++V L IEQEVS V ++
Sbjct: 462 PVLTGSQ-TTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSS 520

Query: 541 GVDISFATRRLTTTVMADSGQIVVLGGLINEEVQESIQKVPFLGDIPILGHLFKSSSSKK 600
+ +F TR + V+ SG+ VV+GGL+++ V ++ KVP LGDIP++G LF+S+S K
Sbjct: 521 DLGATFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKV 580

Query: 601 TKKNLMIFIKPTIIRDGVTMEGIAGRKYNYFRALQLEQQ-ERGVNLMPDTKVPVLDEWNQ 659
+K+NLM+FI+PT+IRD + +Y F Q +Q+ + + M + + + Q
Sbjct: 581 SKRNLMLFIRPTVIRDRDEYRQASSGQYTAFNDAQSKQRGKENNDAMLNQDLLEIYP-RQ 639

Query: 660 SEYLPPEVNDILERYKEGKGL 680
+V+ ++ + G L
Sbjct: 640 DTAAFRQVSAAIDAFNLGGNL 660


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0168BCTERIALGSPF5060.0 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 506 bits (1304), Expect = 0.0
Identities = 232/407 (57%), Positives = 308/407 (75%), Gaps = 1/407 (0%)

Query: 1 MPAFEYKALDAKGKQLKGVIEADTARHARSQLRDQRLMPLDILPVTEKEAKAKSSGFAL- 59
M + Y+ALDA+GK+ +G EAD+AR AR LR++ L+PL + + K+ S+G +L
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 60 FKRGISVAELALITRQIATLVAAGLPIEESLKAVGQQCEKDRLASMIMAVRSRVVEGYSL 119
K +S ++LAL+TRQ+ATLVAA +P+EE+L AV +Q EK L+ ++ AVRS+V+EG+SL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 120 ADSLAEFPHIFDDLYRAMVASGEKSGHLEVVLNRLADYTERRQQLKSKLQQAMIYPIMLT 179
AD++ FP F+ LY AMVA+GE SGHL+ VLNRLADYTE+RQQ++S++QQAMIYP +LT
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 180 LVAIGVVSVLLAAVVPKVVGQFEHMGAELPATTRFLIAASDFVQNYGLLVVLVLGILLVV 239
+VAI VVS+LL+ VVPKVV QF HM LP +TR L+ SD V+ +G ++L L +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 240 FQRLLKSPIFKMKFHTFLLKMPVIGRVSKGLNTARFARTLSILSASSVPLLDGMRIASEV 299
F+ +L+ ++ FH LL +P+IGR+++GLNTAR+ARTLSIL+AS+VPLL MRI+ +V
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 300 LQNMRVRAAVDDATARVREGTSLSTALTNTKLFPAMMLYMIASGEKSGQLEDMLERAADN 359
+ N R + AT VREG SL AL T LFP MM +MIASGE+SG+L+ MLERAADN
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 360 QDREFESNVTLALGVFEPMLVVSMAGVVLFIVMAILQPILALNNLIS 406
QDREF S +TLALG+FEP+LVVSMA VVLFIV+AILQPIL LN L+S
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLMS 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0169BCTERIALGSPG2294e-81 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 229 bits (586), Expect = 4e-81
Identities = 97/144 (67%), Positives = 118/144 (81%)

Query: 1 MQMNKKHKGFTLLEVMVVIVILGILASMVVPNLMGNKDKADQQKAVSDIVALENALDMYK 60
M+ K +GFTLLE+MVVIVI+G+LAS+VVPNLMGNK+KAD+QKAVSDIVALENALDMYK
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYK 60

Query: 61 LDNGVYPTTEQGLEALVQKPTISPEPRNYREEGYVKRLPQDPWRNNYLLLSPGENSKLDI 120
LDN YPTT QGLE+LV+ PT+ P NY +EGY+KRLP DPW N+Y+L++PGE+ D+
Sbjct: 61 LDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDL 120

Query: 121 FSAGPDSQPGTEDDIGNWNLQNFQ 144
SAGPD + GTEDDI NW L +
Sbjct: 121 LSAGPDGEMGTEDDITNWGLSKKK 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0170BCTERIALGSPH831e-22 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 83.1 bits (205), Expect = 1e-22
Identities = 45/171 (26%), Positives = 71/171 (41%), Gaps = 39/171 (22%)

Query: 4 LRHAGFTLMEVMLVILLMGLTAAGVTMSIGNSGPQQALEKTAQQFIAATELVLDETVLSG 63
+R GFTL+E+ML++LLMG++A V ++ S A + T +F A V + +G
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQ-TLARFEAQLRFVQQRGLQTG 59

Query: 64 QFIGIVVEKTSYQFVFYKDG---------------KWNPLEKDRILSEKQMEPGVVINLV 108
QF G+ V +QF+ + +W PL R+ +
Sbjct: 60 QFFGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAGRVATSGS---------- 109

Query: 109 LDGLPLVQEDEQDESWFEEPLIEPSSEDKKKHPEPQILLFPSGEMSAFELS 159
+ G L Q E+W P +L+FP GEM+ F L+
Sbjct: 110 IAGGKLNLAFAQGEAW-------------TPGDNPDVLIFPGGEMTPFRLT 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0171PilS_PF08805300.001 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 30.3 bits (68), Expect = 0.001
Identities = 12/32 (37%), Positives = 18/32 (56%)

Query: 5 RGMTLLEVIVALAVFAVAAVSITKSLSEQMAN 36
+G TL+EV++ + V V A S K S +N
Sbjct: 26 KGATLMEVLLVVGVIVVLAASAYKLYSMVQSN 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0172BCTERIALGSPG365e-05 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 35.6 bits (82), Expect = 5e-05
Identities = 15/40 (37%), Positives = 26/40 (65%), Gaps = 3/40 (7%)

Query: 4 KRTNAHRGFTLLEMLIAIAIFAMLGLAANAVLSTVLTNDE 43
+ T+ RGFTLLE+++ I I +G+ A+ V+ ++ N E
Sbjct: 2 RATDKQRGFTLLEIMVVIVI---IGVLASLVVPNLMGNKE 38


56SO_0281SO_0289N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0281221-4.020544type IV pilus biogenesis protein PilM
SO_0282220-3.722759type IV pilus biogenesis protein PilN
SO_0283019-2.824379type IV pilus biogenesis protein PilO
SO_0284017-2.290624PilQ chaperone PilP
SO_0285117-2.035725type IV pilus secretin PilQ
SO_0286117-0.891516shikimate kinase AroK
SO_0287015-0.7312513-dehydroquinate synthase AroB
SO_0288114-0.006433cell division protein DamX
SO_0289-2160.673858N6-adenine DNA methyltransferase Dam
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0281SHAPEPROTEIN431e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 42.8 bits (101), Expect = 1e-06
Identities = 32/156 (20%), Positives = 57/156 (36%), Gaps = 34/156 (21%)

Query: 199 VDIGANMTTFCVVESGETTFIREQAFGGELFTQSILSFYGMSY------EQAEKAKIE-- 250
VDIG T V+ + GG+ F ++I+++ +Y AE+ K E
Sbjct: 164 VDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIG 223

Query: 251 -------------------GDLPRNY------MFEVLSPFQTQLLQQIKRTLQIYCTSSG 285
+PR + + E L T ++ + L+
Sbjct: 224 SAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELA 283

Query: 286 KDKVDY-LVLCGGTSKLEGMANLLTNELGVHTIIAD 320
D + +VL GG + L + LL E G+ ++A+
Sbjct: 284 SDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAE 319


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0285BCTERIALGSPD2478e-75 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 247 bits (632), Expect = 8e-75
Identities = 99/411 (24%), Positives = 187/411 (45%), Gaps = 38/411 (9%)

Query: 306 GDITLRLDDVPWDQALDLILQTKGLDKRIEGNILMVAPSEELAIRESQNLKNKQEVKELA 365
GD ++ + W A D++ L+K + L + + E N
Sbjct: 190 GDRSVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSR 249

Query: 366 PLYSEYLQ----------------INYAKATDIAELLKGADSSLLSPRG----------- 398
++ + YAKA+D+ E+L G S++ S +
Sbjct: 250 QRIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKN 309

Query: 399 -SVAVDERTNTVLVKDTAEIIENIHRLVEVLDIPIRQVLIESRMVTVKDDVSEDLGIRWG 457
+ +TN ++V +++ ++ R++ LDI QVL+E+ + V+D +LGI+W
Sbjct: 310 IIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWA 369

Query: 458 VTDQQGNKGTSGSLEGAGDIANGKVPSLDNRLNVNLPAAVTNPTSIAFHVAKLADGTILD 517
+ + T+ L + IA + D ++ +L +A+++ IA +
Sbjct: 370 NKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGN----WA 425

Query: 518 LELSALEQENKGEIIASPRITTSNQKAAYIEQGVEIPYV-----QSTSSGATSVTFKKAV 572
+ L+AL K +I+A+P I T + A G E+P + S + +V K
Sbjct: 426 MLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVG 485

Query: 573 LSLRVTPQITPDNRVILDLEITQDSQGKT-VDTPTGPAVAIDTQRIGTQVLVDNGETIVL 631
+ L+V PQI + V+L++E S T + +T+ + VLV +GET+V+
Sbjct: 486 IKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGSGETVVV 545

Query: 632 GGIYQQNLISRVSKVPILGDIPLVGFLFRNTTDKNERQELLIFVTPKIVNE 682
GG+ +++ KVP+LGDIP++G LFR+T+ K ++ L++F+ P ++ +
Sbjct: 546 GGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRD 596



Score = 47.6 bits (113), Expect = 1e-07
Identities = 34/175 (19%), Positives = 75/175 (42%), Gaps = 14/175 (8%)

Query: 275 SLNFQNISVRTVLQIIADYNNFNLVTSDSVEGDITLR-LDDVPWDQALDLILQTKGLDKR 333
S +F+ ++ + ++ N ++ SV G IT+R D + +Q L
Sbjct: 31 SASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVL----D 86

Query: 334 IEGNILMVAPSEELAIRESQNLKNKQEVK--ELAP-----LYSEYLQINYAKATDIAELL 386
+ G ++ + L + S++ K + AP + + + + A D+A LL
Sbjct: 87 VYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVVPLTNVAARDLAPLL 146

Query: 387 KGADSSLLSPRGSVAVDERTNTVLVKDTAEIIENIHRLVEVLDIPIRQVLIESRM 441
+ + + + GSV E +N +L+ A +I+ + +VE +D + ++ +
Sbjct: 147 RQLNDN--AGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSVVTVPL 199


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0286PF05272310.002 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 31.2 bits (70), Expect = 0.002
Identities = 16/64 (25%), Positives = 25/64 (39%), Gaps = 8/64 (12%)

Query: 9 LVGPMGAGKSTIGRHLAQML-----HLEFHDSDQEIEQRTGADIAWVFDVEGEEGFRRRE 63
L G G GKST+ L + H + EQ G +++ FRR +
Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAG---IVAYELSEMTAFRRAD 657

Query: 64 AQVI 67
A+ +
Sbjct: 658 AEAV 661


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0288PF05272340.002 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 33.5 bits (76), Expect = 0.002
Identities = 15/53 (28%), Positives = 22/53 (41%)

Query: 26 SDQLLVLVGAQGSGKTTLLTALATDVDDSNTALVICPMHADNAEIRRKILVQL 78
D +VL G G GK+TL+ L S+T I +I + +L
Sbjct: 595 FDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYEL 647


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0289TYPE3IMSPROT344e-04 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 34.4 bits (79), Expect = 4e-04
Identities = 20/90 (22%), Positives = 31/90 (34%), Gaps = 17/90 (18%)

Query: 164 ISYEKAFEQIRAGDVIYCDPP-------YAPLSTTASFTTYVGAGFSLDDQALLARYSRH 216
I E ++ V+ +P Y T T+ D Q R
Sbjct: 245 IQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYT----DAQVQ---TVRK 297

Query: 217 MALEQRIPVVISNHDIPLTRELYRGAHLAK 246
+A E+ +P++ IPL R LY A +
Sbjct: 298 IAEEEGVPIL---QRIPLARALYWDALVDH 324


57SO_0502SO_0526N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_05020120.447278transcriptional regulator ArsR family
SO_0503-1121.235414permease of unknown function DUF318 ArsP
SO_05040122.318684NAD(P)H-flavin reductase Fre
SO_0505-1142.059855Sel1 repeat protein
SO_05060163.0407623-octaprenyl-4-hydroxybenzoate carboxy-lyase
SO_05081162.909541hypothetical protein
SO_05103182.540210oxidoreductase short-chain
SO_05114171.265284acetyl-CoA carboxylase biotin carboxyl carrier
SO_05124161.7724493-dehydroquinate dehydratase type II AroQ
SO_05134172.619412stop codon-independent peptidyl-tRNA hydrolyzing
SO_05140184.164433protein of unknown function DUF3478
SO_0515-1184.128026DUF3012 domain-containing lipoprotein
SO_0516-1173.997306hypothetical protein
SO_0518-2183.700046heavy metal efflux pump secretin component CzcC
SO_0519-2193.619294heavy metal efflux pump MFP component CzcB
SO_0520-2193.248531heavy metal efflux pump permease component CzcA
SO_0521-1151.354556monooxygenase domain protein
SO_0522-1151.404208large conductance mechanosensitive ion channel
SO_0523-2161.454712transcriptional regulator LysR family
SO_0525-2170.945401multidrug efflux pump permease component RmrB
SO_0526-1180.017504acteyltransferase GNAT family ElaA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0502HTHTETR280.008 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 27.7 bits (61), Expect = 0.008
Identities = 9/50 (18%), Positives = 20/50 (40%), Gaps = 4/50 (8%)

Query: 15 AKVLKELGHPTRLALF----RILVKGGYEGVAVGQLQEELQVPGSTLSHH 60
A+ K+ TR + R+ + G ++G++ + V + H
Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWH 51


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0508IGASERPTASE310.004 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 31.2 bits (70), Expect = 0.004
Identities = 16/78 (20%), Positives = 26/78 (33%)

Query: 143 PTGYDDTPVAISAPVRVTTSMQYSPSEGRMVSNMPSNSATVISAASTARASTVSAEQTVA 202
P +T P + T+S P N ++ + A ++
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSN 1217

Query: 203 VPRARAARSVSSLPSNAR 220
P+ R RSV S+P N
Sbjct: 1218 KPKNRHRRSVRSVPHNVE 1235


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0510DHBDHDRGNASE495e-09 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 48.5 bits (115), Expect = 5e-09
Identities = 41/183 (22%), Positives = 80/183 (43%), Gaps = 5/183 (2%)

Query: 2 ILITGASSGLGAALAALYAKDNQALTLTGRDANRLHTVANALSPFSQQAITAIAADLSDE 61
ITGA+ G+G A+A A + + +L V ++L ++ A A AD+ D
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHA-EAFPADVRDS 69

Query: 62 ASVEALFDGLTDSPA---TVIHCAGSGYFGALENQGAREIKTLLNNNVTSTILLVRELVK 118
A+++ + + +++ AG G + + E + + N T R + K
Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129

Query: 119 RYKNQ-AITVVVVMSTAALAAKAGESTYCAAKWAVRGFIESVRLELKQSPMKLIAVYPGG 177
++ + ++V V S A + + Y ++K A F + + LEL + ++ V PG
Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189

Query: 178 MDT 180
+T
Sbjct: 190 TET 192


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0511RTXTOXIND280.014 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 28.3 bits (63), Expect = 0.014
Identities = 9/23 (39%), Positives = 11/23 (47%)

Query: 126 GIIGAIWVKEGDEVAFDQPLFTL 148
I+ I VKEG+ V L L
Sbjct: 105 SIVKEIIVKEGESVRKGDVLLKL 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0512TRNSINTIMINR270.022 Translocated intimin receptor (Tir) signature.
		>TRNSINTIMINR#Translocated intimin receptor (Tir) signature.

Length = 549

Score = 27.4 bits (60), Expect = 0.022
Identities = 11/27 (40%), Positives = 18/27 (66%), Gaps = 2/27 (7%)

Query: 31 DIVAQLNEQAQAAGVQL--EHIQSNAE 55
DIV Q+ +QA+ AG + ++SNA+
Sbjct: 316 DIVEQIAQQAKEAGEVARQQAVESNAQ 342


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0518RTXTOXIND320.004 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.1 bits (73), Expect = 0.004
Identities = 16/160 (10%), Positives = 48/160 (30%), Gaps = 10/160 (6%)

Query: 76 EVQAQIARQQQAELAIAAADRAVYNPEL-GLNYQNSETDTYTVGLSQTLDWGDKRGVATR 134
+ + QA L + EL L + Y +S+ + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 135 LAQLEAQILLADIRLERSQMLAERLLALAEQAQGQKALTFAEQQLRFTQAQLNIAEQRFA 194
+ + Q ++ L++ + AE+ + E R +++L+
Sbjct: 195 FSTWQNQKYQKELNLDKKR---------AERLTVLARINRYENLSRVEKSRLDDFSSLLH 245

Query: 195 AGDLSDVELQLLKLELASNTADYALAEQAALVAEGKVIEL 234
++ + + + + + + E +++
Sbjct: 246 KQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSA 285


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0519RTXTOXIND553e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 54.8 bits (132), Expect = 3e-10
Identities = 29/138 (21%), Positives = 56/138 (40%), Gaps = 9/138 (6%)

Query: 161 EVAKAQAEYINAAAEWSRVRR---MSEGAVSVSRRMQAQVDAELKRAILEAIKMTSEQIR 217
V + + +Y+ A E + E + ++ V K IL+ ++ T++ I
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 218 TLESKPEA----IGSYQLLAPIDGRVQQ-DIAMLGQVFSAGTPLMQLT-DESHLWVEAQL 271
L + + + AP+ +VQQ + G V + LM + ++ L V A +
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 272 TPAQAVNVKVGAPALIQV 289
+ VG A+I+V
Sbjct: 373 QNKDIGFINVGQNAIIKV 390



Score = 42.5 bits (100), Expect = 2e-06
Identities = 26/149 (17%), Positives = 54/149 (36%), Gaps = 5/149 (3%)

Query: 105 SLTNLNLDVRATATLVVDRDKTATLAPQLDVRVQARHVVPGQEVKKGEPLLTLGG----A 160
L + + A L ++ + P + V+ V G+ V+KG+ LL L A
Sbjct: 76 VLGQVEIVATANGKLTHS-GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 161 EVAKAQAEYINAAAEWSRVRRMSEGAVSVSRRMQAQVDAELKRAILEAIKMTSEQIRTLE 220
+ K Q+ + A E +R + +S D + + E + + +
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQ 194

Query: 221 SKPEAIGSYQLLAPIDGRVQQDIAMLGQV 249
YQ +D + + + +L ++
Sbjct: 195 FSTWQNQKYQKELNLDKKRAERLTVLARI 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0520ACRIFLAVINRP6560.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 656 bits (1693), Expect = 0.0
Identities = 224/1082 (20%), Positives = 434/1082 (40%), Gaps = 74/1082 (6%)

Query: 9 AIKNRLLVVLALLAAVAASVAMLPKLNLDAFPDVTNVQVTINTEAEGLAAEEVEKLISYP 68
I+ + + + + A + +L + +P + V+++ G A+ V+ ++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 69 VESAMYALPAVTEVRSLS-RTGLSIVTVVFAEGTDIYFARQQVFEQLQAAREMIPSGVGV 127
+E M + + + S S G +T+ F GTD A+ QV +LQ A ++P V
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 128 PEIGPNTSGLGQIYQYILRADPSAGINAAELRSLNDYLVKLILMPVGGVTDVLSFGGEVR 187
I S + +D + G ++ VK L + GV DV FG +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSD-NPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ-Y 182

Query: 188 QYQVQVDPNKLRAYGLSMAQVSEALESNNRNAGGWFMDQGQEQLVVRGYGMLPAGEAGLA 247
++ +D + L Y L+ V L+ N + G L + +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLG-GTPALPGQQLNASIIAQTRFK 241

Query: 248 SIAQIPLTEVR----GTPVRVGDIAKVDFGSEIRVGAVTMTRRDEAGQAQALGEVVAGVV 303
+ + +R G+ VR+ D+A+V+ G E + G+ +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARIN-----GK-----PAAGLGI 291

Query: 304 LKRMGANTKATIDDIDARINLIEQALPKGVSFEVFYDQADLVDKAVATVRDALLMAFVFI 363
GAN T I A++ ++ P+G+ YD V ++ V L A + +
Sbjct: 292 KLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLV 351

Query: 364 VVILALFLVNIRATLLVLLSIPVSIGLALMVMSYYGLSANLMSLGGLAVAIGMLVDGSVV 423
+++ LFL N+RATL+ +++PV + +++ +G S N +++ G+ +AIG+LVD ++V
Sbjct: 352 FLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIV 411

Query: 424 MVENIFKHLTQPDRRHLTQARARADGEADPYHGDEDGGVNADDDDHQGNMAVRIMLAAKE 483
+VEN+ R + + P + +
Sbjct: 412 VVENV--------------ERVMMEDKLPPKEA--------------------TEKSMSQ 437

Query: 484 VCSPIFFATAIIIVVFAPLFALEGVEGKLFQPMAVSIILAMISALLVALIAVPALAVYLF 543
+ + ++ VF P+ G G +++ +++I+ AM ++LVALI PAL L
Sbjct: 438 IQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLL 497

Query: 544 K----------RGVVLKESVILRPLDNAYRKLLSATLARPKMVVLSAVIMFVMSMALLPR 593
K G + N Y + L +L ++ + L R
Sbjct: 498 KPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLR 557

Query: 594 LGTEFVPELEEGTINLRVTLAPTASLGTSLDVAPKLEALLLQFPEVEYALSRIGAPELGG 653
L + F+PE ++G + L A+ + V ++ L+ + S
Sbjct: 558 LPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVE-SVFTVNGFSF 616

Query: 654 DPEPVSNIEIYIGLKPIEEWQSASSRLA--LQRLMEEKLSVFPGLLLTFSQPIATRVDEL 711
+ + ++ LKP EE + + R E + G ++ F+ P + EL
Sbjct: 617 SGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMP---AIVEL 673

Query: 712 LSGVKAQLA-IKLFGPDLAVLSDKGQVLTDLVAKIPGAV-DVSLEQVSGEAQLVVRPDRS 769
+ I G L+ L + A+ P ++ V + AQ + D+
Sbjct: 674 GTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQE 733

Query: 770 QLARYGISVDQVMTLVSQGIGGASAGQVIDGNARYDINLRLAAEFRSSPDVIKDLLLSGT 829
+ G+S+ + +S +GG ID + ++ A+FR P+ + L +
Sbjct: 734 KAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSA 793

Query: 830 NGAIVRLGEVASVEVEMAPPNIRRDDVQRRVVVQANVA-GRDMGSIVNDIYALVPKADLP 888
NG +V + P + R + + +Q A G G + + L K LP
Sbjct: 794 NGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASK--LP 851

Query: 889 AGYTVIVGGQYENQQRAQQKLMLVVPVSIALIALLLYFSFGAVKQVLLIMANVPLALIGG 948
AG G ++ + + +V +S ++ L L + + + +M VPL ++G
Sbjct: 852 AGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGV 911

Query: 949 IVALFVSGTYLSVPSSIGFITLFGVAVLNGVVLVDSINQ-RRQSGESLYDSVYEGTVGRL 1007
++A + V +G +T G++ N +++V+ + G+ + ++ RL
Sbjct: 912 LLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRL 971

Query: 1008 RPVLMTALTSALGLIPILVSSGVGSEIQKPLAVVIIGGLFSSTALTLLVLPTLYRWLYRR 1067
RP+LMT+L LG++P+ +S+G GS Q + + ++GG+ S+T L + +P + + R
Sbjct: 972 RPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031

Query: 1068 DK 1069
K
Sbjct: 1032 FK 1033



Score = 99.1 bits (247), Expect = 4e-23
Identities = 92/512 (17%), Positives = 192/512 (37%), Gaps = 37/512 (7%)

Query: 575 MVVLSAVIMFVMSMALLPRLGTEFVPELEEGTINLRVTLAPTASLGTSLD-VAPKLEALL 633
VL+ ++M ++A+L +L P + +++ P A T D V +E +
Sbjct: 12 AWVLAIILMMAGALAIL-QLPVAQYPTIAPPAVSVSAN-YPGADAQTVQDTVTQVIEQNM 69

Query: 634 LQFPEVEYALSRIGAPELGGDPEPVSNIEIYIGLKPIEEWQSASSRLALQRLMEEKLSVF 693
+ Y S + ++ I + + +A ++ KL +
Sbjct: 70 NGIDNLMYMSST---------SDSAGSVTITLTFQS-----GTDPDIAQVQVQN-KLQLA 114

Query: 694 PGLLLTFSQPIATRVDELLSGVKAQLAIKLFGPDL--AVLSDKGQ-VLTDLVAKIPGAVD 750
LL Q V++ S P +SD + D ++++ G D
Sbjct: 115 TPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGD 174

Query: 751 VSLEQVSGEAQLVVRPDRSQLARYGISVDQVMTLVSQGIGGASAGQVIDGNARYDINLRL 810
V L + + + D L +Y ++ V+ + +AGQ+ A L
Sbjct: 175 VQL--FGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNA 232

Query: 811 A----AEFRSSPDVIKDLLLSGTNGAIVRLGEVASVEVEMAPPNIR-RDDVQRRVVVQAN 865
+ F++ + K L ++G++VRL +VA VE+ N+ R + + +
Sbjct: 233 SIIAQTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIK 292

Query: 866 VA-GRDMGSIVNDIYALVP--KADLPAGYTVIVGGQYENQQRAQQKLMLVVP---VSIAL 919
+A G + I A + + P G V+ Y+ Q + VV +I L
Sbjct: 293 LATGANALDTAKAIKAKLAELQPFFPQGMKVLY--PYDTTPFVQLSIHEVVKTLFEAIML 350

Query: 920 IALLLYFSFGAVKQVLLIMANVPLALIGGIVALFVSGTYLSVPSSIGFITLFGVAVLNGV 979
+ L++Y ++ L+ VP+ L+G L G ++ + G + G+ V + +
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAI 410

Query: 980 VLVDSINQRRQS-GESLYDSVYEGTVGRLRPVLMTALTSALGLIPILVSSGVGSEIQKPL 1038
V+V+++ + ++ + ++ A+ + IP+ G I +
Sbjct: 411 VVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQF 470

Query: 1039 AVVIIGGLFSSTALTLLVLPTLYRWLYRRDKR 1070
++ I+ + S + L++ P L L +
Sbjct: 471 SITIVSAMALSVLVALILTPALCATLLKPVSA 502


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0522MECHCHANNEL1691e-57 Bacterial mechano-sensitive ion channel signature.
		>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature.

Length = 136

Score = 169 bits (430), Expect = 1e-57
Identities = 89/136 (65%), Positives = 111/136 (81%), Gaps = 1/136 (0%)

Query: 1 MSLIQEFKAFASRGNVIDMAVGIIIGAAFGKIVSSFVADIIMPPIGIILGGVNFSDLSVV 60
MS+I+EF+ FA RGNV+D+AVG+IIGAAFGKIVSS VADIIMPP+G+++GG++F +V
Sbjct: 1 MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVT 60

Query: 61 LLAAQGDAPAVVIAYGKFIQTVIDFTIIAFAIFMGLKAINSLKRKQEEAPPASPAPTKDQ 120
L AQGD PAVV+ YG FIQ V DF I+AFAIFM +K IN L RK+EE P A+PAPTK++
Sbjct: 61 LRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEE-PAAAPAPTKEE 119

Query: 121 ELLSEIRDLLKAQQEK 136
LL+EIRDLLK Q +
Sbjct: 120 VLLTEIRDLLKEQNNR 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0525TCRTETB1232e-32 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 123 bits (310), Expect = 2e-32
Identities = 86/400 (21%), Positives = 168/400 (42%), Gaps = 17/400 (4%)

Query: 42 AFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIAIPLSGWLSTGLSVRRYL 101
+F ++L+ + N S+ +I +W++TA+++ I + G LS L ++R L
Sbjct: 23 SFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLL 82

Query: 102 LWTTAAFIFASILCSISWNLES-MIAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMA 160
L+ F S++ + + S +I R +QG A L ++ ++P+ R
Sbjct: 83 LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFG 142

Query: 161 LFGVTATFAPSIGPTLGGWLTEHFSWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKN 220
L G +GP +GG + + W YL +P ++ L K+ V +
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPMITIITVPFLMKLLKKEVRIKG--H 198

Query: 221 ADLAGIVTMALGMGCLEVVLEEGNRKDWFGSDLIRNLAIIAAVNLVLFVWIQLNRKDPLV 280
D+ GI+ M++G+ + F + + I++ ++ ++FV DP V
Sbjct: 199 FDIKGIILMSVGIVFFML----------FTTSYSISFLIVSVLSFLIFVKHIRKVTDPFV 248

Query: 281 NLRLLGKRDFVLSTVAYFLLGMALFGAIYLIPLYLSQVHDYTPLEIGEVIMWMGFPQLLV 340
+ L F++ + ++ + G + ++P + VH + EIG VI++ G +++
Sbjct: 249 DPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVII 308

Query: 341 L-PLVPRLMQRFDGRYLAAFGFFMFALSYYMNSQMTADYAGQQMIASQVVRALGQPFILV 399
+ L+ R Y+ G ++S+ S + M V G F
Sbjct: 309 FGYIGGILVDRRGPLYVLNIGVTFLSVSFLTAS-FLLETTSWFMTIIIVFVLGGLSFTKT 367

Query: 400 PIGMLATAHLKPHENPSASTVLNVMRNLGGAFGIALVATL 439
I + ++ LK E + ++LN L GIA+V L
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGL 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0526SACTRNSFRASE371e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.8 bits (85), Expect = 1e-05
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 7/73 (9%)

Query: 81 ASIGRVVISPAGRGQGLATPLMQQAIEAALTAWPEAGIQIGAQEYLNA----FYQKLGFH 136
A I + ++ R +G+ T L+ +AIE A G+ + Q+ +N FY K F
Sbjct: 90 ALIEDIAVAKDYRKKGVGTALLHKAIEWAKEN-HFCGLMLETQD-INISACHFYAKHHFI 147

Query: 137 ACS-EVYLEDGIP 148
+ + L P
Sbjct: 148 IGAVDTMLYSNFP 160


58SO_4768SO_0549N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4768113-2.557135two component signal transduction system
SO_0545013-2.354808two component signal transduction system
SO_0546-115-1.484012ribosomal protein S6 glutaminyl transferase
SO_0547-216-1.585996putative outer membrane protein TIGR02001
SO_0548-215-2.073855histone-like DNA-binding protein
SO_0549-215-2.468099two component signal transduction system
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4768HTHFIS444e-08 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 44.1 bits (104), Expect = 4e-08
Identities = 17/73 (23%), Positives = 29/73 (39%), Gaps = 6/73 (8%)

Query: 11 TILLVDDDDVDYIAVQRAMKRLRLLNPLVRARDGLEALTILTNTDAIKGAYLILLDLNMP 70
TIL+ DDD + +A+ R + + + A L++ D+ MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGY--DVRITSNAATLWRWI----AAGDGDLVVTDVVMP 58

Query: 71 RMNGFEFLEHIRS 83
N F+ L I+
Sbjct: 59 DENAFDLLPRIKK 71


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0545HTHFIS631e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.9 bits (153), Expect = 1e-12
Identities = 26/102 (25%), Positives = 48/102 (47%), Gaps = 3/102 (2%)

Query: 3 LLLIDDDEVDRTAIIRALRQSKLAFNVVEANCAFDGLNLALQRHFDGILLDYLLPDANGL 62
+L+ DDD RT + +AL S+ ++V + A D ++ D ++PD N
Sbjct: 6 ILVADDDAAIRTVLNQAL--SRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 63 EVLIKLNSMTQEQTVVVMLSRYEDEKLAQRCIELGAQDFLLK 104
++L ++ + V+++S A + E GA D+L K
Sbjct: 64 DLLPRIKKA-RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPK 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0548DNABINDINGHU1102e-35 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 110 bits (276), Expect = 2e-35
Identities = 43/88 (48%), Positives = 64/88 (72%)

Query: 2 NKTELIAKIAENADITKAQATRALKSFEAAITESMKNGDKISIVGFGSFETTTRAARTGR 61
NK +LIAK+AE ++TK + A+ + +A++ + G+K+ ++GFG+FE RAAR GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEIQIAEATVPKFKAGKTLRDSV 89
NPQTG+EI+I + VP FKAGK L+D+V
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0549HTHFIS632e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.5 bits (152), Expect = 2e-13
Identities = 24/107 (22%), Positives = 44/107 (41%), Gaps = 3/107 (2%)

Query: 146 RVLVVDDSRMARNVIKRTIGNLGIKLITEAEDGAQAIELMRHNMFDLIITDYNMPSVDGL 205
+LV DD R V+ + + G + + A + DL++TD MP +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 206 ALTQFIRSESQQSHVPILMVSSEANDAHLSNVSQAGVNALCDKPFEP 252
L I+ + +P+L++S++ S+ G KPF+
Sbjct: 64 DLLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDL 108



Score = 47.9 bits (114), Expect = 2e-08
Identities = 30/155 (19%), Positives = 58/155 (37%), Gaps = 6/155 (3%)

Query: 10 SMLIVEPSETQRRIIIKCLQQEGIVSIEHAANIAEAKGLIARHKPDLIASAMHFEDGTAT 69
++L+ + R ++ + L + G + +N A IA DL+ + + D A
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGY-DVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 70 EFLSYLRSKSEYKDIQFMLVSSECRREQLESFRQSGVVAILPKPFNAEHLGKALNATIDL 129
+ L ++ D+ +++S++ + G LPKPF+ L + +
Sbjct: 64 DLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 130 LSHDELDLSHFDVHDVRVLVVDDSRM--ARNVIKR 162
L D D LV + M V+ R
Sbjct: 122 PKRRPSKLED-DSQDGMPLVGRSAAMQEIYRVLAR 155


59SO_0619SO_0627N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0619-2120.303652succinylglutamic semialdehyde dehydrogenase
SO_0620-112-0.756704putative hydrolase involved in arginine and
SO_0621-214-0.623893two component signal transduction system
SO_0622-116-0.756248two component signal transduction system
SO_0623-214-0.048695hypothetical protein
SO_0624-2151.979508cAMP-responsive regulator of catabolite
SO_0625-1162.913512periplasmic cyctochrome c oxidase regulatory
SO_0627-1172.9240752'-5' RNA ligase LigT
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0619DNABINDINGHU290.012 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 28.9 bits (65), Expect = 0.012
Identities = 9/32 (28%), Positives = 17/32 (53%)

Query: 74 ANKAELAETIAQETGKPQWETATEVAAMIGKI 105
ANK +L +A+ T + ++A V A+ +
Sbjct: 2 ANKQDLIAKVAEATELTKKDSAAAVDAVFSAV 33


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0621PF06580290.039 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.1 bits (65), Expect = 0.039
Identities = 19/123 (15%), Positives = 45/123 (36%), Gaps = 25/123 (20%)

Query: 326 NFELDPSIQRLPIHREDGMELLGNLLDNAFKWA------DSQVTVKLWKAQNKLYLSIDD 379
+++P+I + + L+ L++N K ++ +K K + L +++
Sbjct: 243 ENQINPAIMDVQVPPM----LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVEN 298

Query: 380 DGPGVADDELDKLTQRGTRLDESVMGHGLGLSIVKE-IAEQYGIELKFTHSRRLSGLCIE 438
G + + G GL V+E + YG E + S + +
Sbjct: 299 TGSLALKNTKE--------------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM 344

Query: 439 LVL 441
+++
Sbjct: 345 VLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0622HTHFIS845e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 84.1 bits (208), Expect = 5e-21
Identities = 34/118 (28%), Positives = 59/118 (50%), Gaps = 1/118 (0%)

Query: 2 KLLLVEDNPMLVSELEKQLKQAGYVTDITDKALEADYLVKETQYDCVILDIGLPDGNGLE 61
+L+ +D+ + + L + L +AGY IT A + D V+ D+ +PD N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 LLEGWRNQGISTPVIMLTARSQWHEKVEGFNAGADDYLGKPFHAQELLARI-QALIHR 118
LL + PV++++A++ + ++ GA DYL KPF EL+ I +AL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0627MPTASEINHBTR280.017 Metalloprotease inhibitor signature.
		>MPTASEINHBTR#Metalloprotease inhibitor signature.

Length = 122

Score = 27.7 bits (61), Expect = 0.017
Identities = 8/37 (21%), Positives = 13/37 (35%), Gaps = 5/37 (13%)

Query: 88 CCLKGDMVSPALALLASQ-----AQNLAQQLQLHQSE 119
C + VS +AS +A QL + +
Sbjct: 10 CVWQVLFVSAGAQAMASSFVVPSTAQMAGQLGIEATG 46


60SO_0853SO_0860N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_08530172.271112PilD processed protein
SO_0854-1110.602345PilD processed protein
SO_0855-1120.529275hydroxyneurosporene synthase AttH
SO_0856-1110.483862ABC-type export system permease component AttFG
SO_0857-2100.171376ABC-type export system ATPase component AttE
SO_0858-210-0.075786Na(+)-linked D-alanine glycine transporter GlyP
SO_0859-210-0.788130two component signal transduction system hybrid
SO_0860015-0.341361two component signal transduction system
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0853BCTERIALGSPG433e-08 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 43.3 bits (102), Expect = 3e-08
Identities = 17/25 (68%), Positives = 22/25 (88%)

Query: 8 GFTLVELMVVIAIIGILASLALPSY 32
GFTL+E+MVVI IIG+LASL +P+
Sbjct: 9 GFTLLEIMVVIVIIGVLASLVVPNL 33


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0854BCTERIALGSPG571e-13 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 57.2 bits (138), Expect = 1e-13
Identities = 28/105 (26%), Positives = 49/105 (46%), Gaps = 10/105 (9%)

Query: 5 QKGFTLIELMIAVAIIGILAAIAIPSFNEYLKQGRRFDAQQYLVTSAQALERHYSRNGLY 64
Q+GFTL+E+M+ + IIG+LA++ +P+ ++ + A +V AL+ + N Y
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLDNHHY 66

Query: 65 PASQ----SLANSPYYSFSYTPTADKFGFSLKAVPTNRQSDPCGT 105
P + SL +P +K +P +DP G
Sbjct: 67 PTTNQGLESLVEAPTL--PPLAANYNKEGYIKRLP----ADPWGN 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0859HTHFIS781e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 77.9 bits (192), Expect = 1e-16
Identities = 31/133 (23%), Positives = 57/133 (42%), Gaps = 1/133 (0%)

Query: 1279 LDGMSILVADDNATARDIMRTTLESMGFNVDTVRSGDEAIMRCSQQEYAVALIDWKMPNL 1338
+ G +ILVADD+A R ++ L G++V + + + + + D MP+
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 1339 DGIETAKQIKQQTKNAPRILMVSAHANQDFLTQIEQLGLAGYISKPISASRLLDGIMNAL 1398
+ + +IK+ + P ++M SA + + G Y+ KP + L+ I AL
Sbjct: 61 NAFDLLPRIKKARPDLPVLVM-SAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 1399 GRSGILPVRRHQD 1411
P + D
Sbjct: 120 AEPKRRPSKLEDD 132



Score = 68.3 bits (167), Expect = 1e-13
Identities = 25/103 (24%), Positives = 42/103 (40%), Gaps = 2/103 (1%)

Query: 1425 RILLVEDNEMNLEVATEFLEQVGIILSIATNGQIALDKLAQQSFDLVLMDCQMPVMDGYQ 1484
IL+ +D+ V + L + G + I +N +A DLV+ D MP + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 1485 ATKAIRQRPELAQLPVIAMTANAMAGDKEMCLRAGMNDHIAKP 1527
I++ LPV+ M+A G D++ KP
Sbjct: 65 LLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKP 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0860HTHFIS883e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 87.6 bits (217), Expect = 3e-21
Identities = 37/159 (23%), Positives = 63/159 (39%), Gaps = 6/159 (3%)

Query: 1 MDKATILVVDDTPENIDILVGILG-EDYKVKVAIDGPRALALVTKTLPDLILLDVMMPGM 59
M ATILV DD +L L Y V++ + + DL++ DV+MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 NGYEVCKLLKQEPLTCHIPVIFVTALSEVADEAQGFALGAVDYITKPVSAPVVKARVKTH 119
N +++ +K+ +PV+ ++A + + GA DY+ KP + +
Sbjct: 61 NAFDLLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 120 LALYDQKRLLEQQVKERTQEL--EETRF-EIIRRLGRAA 155
LA ++ + + L EI R L R
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLM 157


61SO_0867SO_0874N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_0867-1121.360639collagenolytic serine protease
SO_08680151.572173putative periplasmic protein of unknown
SO_08690152.486083pantoate--beta-alanine ligase PanC
SO_0870-1162.2715733-methyl-2-oxobutanoate hydroxymethyltransferase
SO_0871-1142.7965182-amino-4-hydroxy-6-
SO_0872-1132.835258polyA polymerase PcnB
SO_0873-2142.573110glutamyl-Q-tRNA-Asp synthetase YadB
SO_0874-2162.234966RNA polymerase-binding protein DksA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0867SUBTILISIN2033e-61 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 203 bits (519), Expect = 3e-61
Identities = 100/293 (34%), Positives = 132/293 (45%), Gaps = 42/293 (14%)

Query: 128 WGMNNTGQSGGTADADIDAPEAWEITTGSSDVVIGVIDTGVDYNHPDLQANMWVNAGEIA 187
N G I AP W T G V + V+DTG D +HPDL+A +
Sbjct: 16 EQQVNEIPRGVEM---IQAPAVWNQTRGR-GVKVAVLDTGCDADHPDLKARI-------- 63

Query: 188 GNGIDDDANGVIDDIHGYSAVNNNGN----PMDGNGHGTHVSGTIGAKGNNGVGVVGVNW 243
I G + +++ D NGHGTHV+GTI A N GVVGV
Sbjct: 64 --------------IGGRNFTDDDEGDPEIFKDYNGHGTHVAGTIAA-TENENGVVGVAP 108

Query: 244 DVKIAGCQFLDTDGYGSTAGAIACIDYFTNLKVNHGVDIKATNNSWGGGGFSQALKDAIE 303
+ + + L+ G G I I Y + VDI + S GG L +A++
Sbjct: 109 EADLLIIKVLNKQGSGQYDWIIQGIYYA----IEQKVDI--ISMSLGGPEDVPELHEAVK 162

Query: 304 AGGEAGILFVAAAGNDAVDND--ASPHYPSSYNSDVVFSIASTTRNDRMSDFSQWGLTSV 361
+ IL + AAGN+ +D YP YN V S+ + + S+FS V
Sbjct: 163 KAVASQILVMCAAGNEGDGDDRTDELGYPGCYNE--VISVGAINFDRHASEFSNSN-NEV 219

Query: 362 DMGAPGSAILSTVRGGGYATYSGTSMATPHVTGAAALVWALNPDLTPVEMKEL 414
D+ APG ILSTV GG YAT+SGTSMATPHV GA AL+ L ++ E
Sbjct: 220 DLVAPGEDILSTVPGGKYATFSGTSMATPHVAGALALIKQLANASFERDLTEP 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0869LPSBIOSNTHSS270.049 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 27.1 bits (60), Expect = 0.049
Identities = 6/26 (23%), Positives = 13/26 (50%)

Query: 34 HQGHITLVKEAAKKCDHVVASIFVNP 59
GH+ +++ + D V ++ NP
Sbjct: 13 TFGHLDIIERGCRLFDQVYVAVLRNP 38


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0873PF04605290.010 Virulence-associated protein D (VapD)
		>PF04605#Virulence-associated protein D (VapD)

Length = 125

Score = 29.1 bits (65), Expect = 0.010
Identities = 8/52 (15%), Positives = 17/52 (32%), Gaps = 2/52 (3%)

Query: 65 AADDILRTLEAYGFEWDDTVLYQSART--DAYQAKLDQLLAQDDAYFCQCSR 114
I + + GFE Y S + ++ L + + +C +
Sbjct: 27 PYSLIKKFMLENGFEHRQYSGYTSKEPINERRVIRIVNKLTKKFTWLGECVK 78


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_0874INVEPROTEIN270.031 Salmonella/Shigella invasion protein E (InvE) signat...
		>INVEPROTEIN#Salmonella/Shigella invasion protein E (InvE)

signature.
Length = 372

Score = 27.0 bits (59), Expect = 0.031
Identities = 22/81 (27%), Positives = 33/81 (40%), Gaps = 13/81 (16%)

Query: 24 GEEYMNAKQLGHFKTILEAWRNQLREEVDRTLSHMQDEAANFPDPVDRAAQEEEFSLELR 83
E AKQ+ ++ L + + + S FPDP D E LR
Sbjct: 88 DEALPKAKQILKLISVH---GGALEDFLRQARSL-------FPDPSDLVLVLREL---LR 134

Query: 84 ARDRERKLIKKIEKTLQKIEE 104
+D E + KK+E L+ +EE
Sbjct: 135 RKDLEEIVRKKLESLLKHVEE 155


62SO_1158SO_1165N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1158-2172.207204DNA-binding ferritin-like protein (oxidative
SO_1159-1181.532100DNA polymerase III psi subunit HolD
SO_11600160.984436ribosomal-protein-alanine acetyltransferase
SO_11610160.214161lipoic acid synthetase LipA
SO_1162016-0.609367lipoyl(octanoyl) transferase LipB
SO_1163115-0.483046protein lipolyation system protein YbeD
SO_11641150.039809D-alanyl-D-alanine carboxypeptidase DacA
SO_11652140.443255septal ring lipoprotein RlpA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1158HELNAPAPROT1372e-44 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 137 bits (346), Expect = 2e-44
Identities = 50/142 (35%), Positives = 74/142 (52%)

Query: 12 EEIAAGLNQLLADSYSLYLKTHSFHWNVTGPMFTSLHLLFEQQYTELALAVDLIAERVRA 71
+ LN L++ + LY K H FHW V GP F +LH FE+ Y A VD IAER+ A
Sbjct: 11 TLVENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLA 70

Query: 72 LGARALGSYSAYAKLTEIHEDQGVTKAETMIRELLSDQEVVIRNARALYPLVSQANDEAT 131
+G + + + Y + I + T A M++ L++D + + ++ + L + D AT
Sbjct: 71 IGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYKQISSESKFVIGLAEENQDNAT 130

Query: 132 ADLLTQRIQIHEKNAWMLRSLL 153
ADL I+ EK WML S L
Sbjct: 131 ADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1160SACTRNSFRASE482e-09 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 47.6 bits (113), Expect = 2e-09
Identities = 18/87 (20%), Positives = 32/87 (36%), Gaps = 10/87 (11%)

Query: 56 LTHTSGQLFGFAIIQQIVDEV----------TLLDICLVPAEQGQGFGRLLLDAIIEDAK 105
+ F + + + + + DI + + +G G LL IE AK
Sbjct: 60 VEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAK 119

Query: 106 NAGAVVVMLEVRESNLAARALYQNRGF 132
+MLE ++ N++A Y F
Sbjct: 120 ENHFCGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1164BLACTAMASEA290.029 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 29.0 bits (65), Expect = 0.029
Identities = 20/109 (18%), Positives = 39/109 (35%), Gaps = 3/109 (2%)

Query: 37 AAKAYVLMDYYSGQIIAEENAYESLNPASLTKMMTSYVIGQEIKAGNVSPDDDVTISKNA 96
+ MD SG+ + A E S K++ + + AG+ + + +
Sbjct: 38 GRVGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQD 97

Query: 97 WSKNFSDSSKMFIEVGKTVKVSDLNRGIIIQSGNDACVAMAEHIAGTEG 145
++S S+ + + V +L I S N A + + G G
Sbjct: 98 L-VDYSPVSEKH--LADGMTVGELCAAAITMSDNSAANLLLATVGGPAG 143


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1165adhesinb290.013 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 29.4 bits (66), Expect = 0.013
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 8/68 (11%)

Query: 10 LLMLSCCFVLAACSSSNSASASASNKNKNMEPNKGRYSL-KN---DKMPLN---PPNVD- 61
+L+L LAACSS S++ + S+K + N + KN DK+ L+ P D
Sbjct: 8 VLLLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSIVPVGQDP 67

Query: 62 HVPNATPK 69
H P+
Sbjct: 68 HEYEPLPE 75


63SO_1193SO_1204N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_11930130.631003preprotein translocase subunit SecD
SO_11940160.583530preprotein translocase subunit SecF
SO_11951160.780822putative RNA-binding protein YhbY
SO_11961170.84546223S rRNA (uridine2552-2'-O)-methyltransferase
SO_11970170.481667ATP-dependent zinc metalloprotease FtsH
SO_11981160.270430dihydropteroate synthase FolP
SO_11994260.820345phosphoglucosamine mutase GlmM
SO_12006290.844083triosephosphate isomeraseTpiA
SO_12016291.240991preprotein translocase subunit SecG
SO_12027311.429395**ribosome maturation factor RimP
SO_12035240.562743N utilization substance protein A NusA
SO_12045250.098829translation initiation factor IF-2 InfB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1193SECFTRNLCASE772e-17 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 77.2 bits (190), Expect = 2e-17
Identities = 31/172 (18%), Positives = 82/172 (47%), Gaps = 4/172 (2%)

Query: 436 VTIVEERTIGPTLGAENIENGFAALGLGMGITLLFMALWYR-RLGWVANVALISNMVILF 494
+ I ++GP + E + +L + + ++ + + + A VAL+ ++++
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 495 GLLALIPGAVLTLPGIAGLVLTVGMAVDTNVLIFERIKDKLKEGRSFALA--IDTGFDSA 552
GL A++ L +A L+ G +++ V++F+R+++ L + ++ L ++ +
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 553 FSTIFDANFTTMITAVVLYSIGNGPIQGFALTLGLGLLTSMFTGIFASRALI 604
S TT++ V + G I+GF + G+ T ++ ++ ++ ++
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIV 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1194SECFTRNLCASE2372e-79 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 237 bits (607), Expect = 2e-79
Identities = 91/299 (30%), Positives = 153/299 (51%), Gaps = 20/299 (6%)

Query: 2 KNINLTKWRYISSAISIFLMLASLTIVCVKGFNWGLDFTGGVVTEVQLDRKITSSELQPL 61
N + +W++ + +I +M+AS+ + V G N+G+DF GG + I +
Sbjct: 12 TNFDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAA 71

Query: 62 LNAAYQQDVSVISASEP--------------------GRWVLRYADTAQSHVDIAQTLAP 101
L DV + +P G + A
Sbjct: 72 LEPLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAV 131

Query: 102 LGEVQVLNTSVVGPQVGKELAEQGGLALLVAMLAILGYLSYRFEWRLASGALFALVHDVM 161
+++ + VGP+V EL +LL A + I+ Y+ RFEW+ A GA+ ALVHDV+
Sbjct: 132 DPALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVL 191

Query: 162 FVLAFFSLTQMEFNLTVLAAVLAILGYSLNDSIIIADRIRELLIAKPKLAIQEINNQAIV 221
+ F++ Q++F+LT +AA+L I GYS+ND++++ DR+RE LI + ++++ N ++
Sbjct: 192 LTVGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVN 251

Query: 222 ATFSRTMVTSGTTLMTVGALWIMGGGPLEGFSIAMFIGILTGTFSSISVGTSLPEFLGL 280
T SRT++T TTL+ + + I GG + GF AM G+ TGT+SS+ V ++ F+GL
Sbjct: 252 ETLSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIVLFIGL 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1197HTHFIS340.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 0.002
Identities = 23/82 (28%), Positives = 32/82 (39%), Gaps = 18/82 (21%)

Query: 193 VLMVGPPGTGKTLLAKAIAGESK---VPFFT-----ISGSDFVEMFVGV------GASRV 238
+++ G GTGK L+A+A+ K PF I G GA
Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTR 222

Query: 239 RD-MFEQAKKSAPCIIFIDEID 259
FEQA+ +F+DEI
Sbjct: 223 STGRFEQAEGGT---LFLDEIG 241


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1200adhesinb310.003 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 31.4 bits (71), Expect = 0.003
Identities = 18/95 (18%), Positives = 34/95 (35%), Gaps = 16/95 (16%)

Query: 142 REARRTFEVIAEELDIVIQKNGTMAFDNAIIAY----EPLWAVGTGKSATPEQAQEVHAF 197
+EA+ F I E +++ G F AY +W + T + TP+Q + +
Sbjct: 186 KEAKEKFNNIPGEKKMIVTSEG--CFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTLVEK 243

Query: 198 IRKRLSEVSPFIGENIRILYGGSVTPSNAADLFAQ 232
+RK + L+ S ++
Sbjct: 244 LRKT----------KVPSLFVESSVDDRPMKTVSK 268


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1201SECGEXPORT1182e-38 Protein-export SecG membrane protein signature.
		>SECGEXPORT#Protein-export SecG membrane protein signature.

Length = 110

Score = 118 bits (298), Expect = 2e-38
Identities = 63/111 (56%), Positives = 82/111 (73%), Gaps = 1/111 (0%)

Query: 1 MYEVLVVVYLLVALGLIGLILIQQGKGADMGASFGAGASGTLFGSSGSGNFLTRTTAILA 60
MYE L+VV+L+VA+GL+GLI++QQGKGADMGASFGAGAS TLFGSSGSGNF+TR TA+LA
Sbjct: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60

Query: 61 IAFFTLSLLIGNLSANHAKNEDAWKNLGSDTEQVTQPVEQGTEKSETKIPD 111
FF +SL++GN+++N W+NL S + Q K + IP+
Sbjct: 61 TLFFIISLVLGNINSNKTNKGSEWENL-SAPAKTEQTQPAAPAKPTSDIPN 110


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1204TCRTETOQM711e-14 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 71.0 bits (174), Expect = 1e-14
Identities = 51/202 (25%), Positives = 77/202 (38%), Gaps = 30/202 (14%)

Query: 392 IMGHVDHGKTSLLDYIRRAKVAAGEAG------------------GITQHIGAYHVETEN 433
++ HVD GKT+L + + A E G GIT G + EN
Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67

Query: 434 GMITFLDTPGHAAFTAMRARGAKATDIVVLVVAADDGVMPQTIEAIQHAKAGNVPLIVAV 493
+ +DTPGH F A R D +L+++A DGV QT + +P I +
Sbjct: 68 TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFI 127

Query: 494 NKMDKPEADIDRV----KSELSQHGVMS-------EDWGGDNMFAFVSAKTGAGVDDLLE 542
NK+D+ D+ V K +LS V+ + + G DDLLE
Sbjct: 128 NKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLE 187

Query: 543 GILLQAEVLELKAVRDGMAAGV 564
+ + LE + +
Sbjct: 188 K-YMSGKSLEALELEQEESIRF 208


64SO_1556SO_1559N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1556113-1.445646putative exonuclease RdgC
SO_1557116-1.568174outer membrane phosphoporin
SO_1558013-1.240428phosphate-responsive two component signal
SO_1559-113-0.980019phosphate-responsive two component signal
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1556SECA310.006 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 31.0 bits (70), Expect = 0.006
Identities = 10/41 (24%), Positives = 25/41 (60%), Gaps = 1/41 (2%)

Query: 81 ESLEEKVAQIEDEENRKLAKKEKDALKD-EIITSLLPRAFS 120
++E ++ ++ DEE + + + L+ E++ +L+P AF+
Sbjct: 29 NAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFA 69


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1557ECOLNEIPORIN828e-20 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 81.8 bits (202), Expect = 8e-20
Identities = 81/346 (23%), Positives = 131/346 (37%), Gaps = 47/346 (13%)

Query: 6 KTLLASALASATLASAYAAEPLTVYGKLNV---TAQSNDENGDAT------TTIQSNASR 56
K+L+A LA+ +A A +T+YG + T++S NG T I S+
Sbjct: 3 KSLIALTLAALPVA---AMADVTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLGSK 59

Query: 57 FGVKGDFELSSSLSAFYTVEYQVDTGAASSDNFTARNQFVGLKGDFGSFSVGRNDTLLKI 116
G KG +L + L A + VE + S R F+GLKG FG VGR +++LK
Sbjct: 60 IGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGN-RQSFIGLKGGFGKLRVGRLNSVLK- 117

Query: 117 SQGDVDQFNDLSGDLG--KLFKGEVRAAQTATYLTPSFGDFVFGVTYVAEGNDVKSQFAQ 174
GD++ ++ S LG K+ + E R Y +P F V Y A ++
Sbjct: 118 DTGDINPWDSKSDYLGVNKIAEPEARLIS-VRYDSPEFAGLSGSVQY-ALNDNAGRH-NS 174

Query: 175 DGFSVAAMY--GDAKLKKTAVYAALAYDSDVSG---YEVLRASLQAKLAGIKLGGMYQQQ 229
+ + Y G ++ Y + Y++ R + QQQ
Sbjct: 175 ESYHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIEKYQIHRLVSGYDNDALYASVAVQQQ 234

Query: 230 EQT-YKLDKTTSLPVEVTAESA---------TGYLLSAAYDINAVTLKAQFQDMEDLGDS 279
+ + + + + EV A A Y +A + + D
Sbjct: 235 DAKLVEENYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDAT-------NYNNDYDQ 287

Query: 280 WSVGADYNLGKPTKVFAFYTNRSLEANTDDDKYI----AIGLEHKF 321
VGA+Y+ K T L+ + K++ +GL HKF
Sbjct: 288 VVVGAEYDFSKRTSALVSA--GWLQEGKGESKFVSTAGGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1558HTHFIS911e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 91.4 bits (227), Expect = 1e-23
Identities = 34/130 (26%), Positives = 64/130 (49%), Gaps = 4/130 (3%)

Query: 3 ARILIVEDELAIREMLTFVMEQHGFTTSAAEDFDSAIALLKEPYPDLILLDWMFPGGSGI 62
A IL+ +D+ AIR +L + + G+ + + + DL++ D + P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 63 QLAKRLKQDEFTRQIPIIMLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVL-- 120
L R+K+ +P+++++A+ +K E GA DY+ KPF EL+ I L
Sbjct: 64 DLLPRIKKAR--PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 121 RRSAPTRLEE 130
+ P++LE+
Sbjct: 122 PKRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1559PF06580330.002 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.002
Identities = 31/189 (16%), Positives = 62/189 (32%), Gaps = 34/189 (17%)

Query: 246 ALMQQQTQRMQSMVEQLLVLSRIEDAADIDLENTVNMSQLMDVLK---EEAKALAKDKYE 302
AL+ + + + M+ L L R + V+++ + V+ + A +D+ +
Sbjct: 184 ALILEDPTKAREMLTSLSELMRY--SLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQ 241

Query: 303 LSFHCEPGLDSHGNELQLRSACSNLISNAIRY----TEPGGKISVQWRSVATGGLFSVAD 358
P + + L+ N I++ GGKI ++ V +
Sbjct: 242 FENQINPAIM---DVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVEN 298

Query: 359 TGEGIAPQHISRLTERFYRVDSARSRQTGGSGLGLAIVKHALSHHHSE---LNISSELGK 415
TG +G GL V+ L + + +S + GK
Sbjct: 299 TGSLALKN------------------TKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGK 340

Query: 416 GSTFSFVIP 424
+ +IP
Sbjct: 341 VNAM-VLIP 348


65SO_1795SO_1802N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1795318-0.400290ATP-dependent Clp protease ATP-binding subunit
SO_1796318-0.450414ATP-dependent protease La Lon
SO_1797217-0.649563histone-like DNA-binding protein HupB
SO_1798114-0.472133peptidyl-prolyl cis-trans isomerse PpiD
SO_1799-114-0.530618molybdenum-pterin binding protein MopI
SO_1800-214-0.722141enoyl acyl-carrier-protein reductase FabV
SO_1801-114-0.669888ABC-type peptide uptake system ATPase component
SO_1802013-0.650130ABC-type peptide uptake system ATPase component
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1795HTHFIS310.009 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.009
Identities = 14/70 (20%), Positives = 28/70 (40%), Gaps = 13/70 (18%)

Query: 64 KLPTPHELRAHLDDYVIGQDRAKKVLSVAVYNHYKRLKNSSPKDGVELGKSNILLIGPTG 123
+ P+ E + ++G+ A +Y RL + +++ G +G
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAA----MQEIYRVLARLMQT---------DLTLMITGESG 170

Query: 124 SGKTLLAETL 133
+GK L+A L
Sbjct: 171 TGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1796HTHFIS330.006 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 32.9 bits (75), Expect = 0.006
Identities = 40/211 (18%), Positives = 77/211 (36%), Gaps = 37/211 (17%)

Query: 262 NMPSDAKEKAVAELNKLRMMSP---MSAEATV---VRSY----VDWMTSVPWSQRSKIKR 311
MP + + + K R P MSA+ T +++ D++ P+ I
Sbjct: 56 VMPDENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPK-PFDLTELIGI 114

Query: 312 DLAKAQEVLDTDHYGLEKVKDRILEYLAVQSRVRQLKGPI---------LCLVGPPGVGK 362
+A LE + + + ++++ + L + G G GK
Sbjct: 115 I-GRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGK 173

Query: 363 TSLGQSIAKATGRK---YVRVALGGVRD---EAEIRGHRRTYIGSMPGKVIQKMAKVGVK 416
+ +++ R+ +V + + + E+E+ GH + G+ G + +
Sbjct: 174 ELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEK---GAFTGAQTRSTGRFEQA 230

Query: 417 N--PLFLLDEIDKMSSDMRGDPASALLEVLD 445
LFL DEI M D + + LL VL
Sbjct: 231 EGGTLFL-DEIGDMPMDAQ----TRLLRVLQ 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1797DNABINDINGHU1194e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 119 bits (300), Expect = 4e-39
Identities = 53/88 (60%), Positives = 69/88 (78%)

Query: 2 NKSELIEKIASGADISKAAAGRALDSFIAAVTEGLKEGDKISLVGFGTFEVRERAERTGR 61
NK +LI K+A +++K + A+D+ +AV+ L +G+K+ L+GFG FEVRERA R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGEEIKIAAAKIPAFKAGKALKDAV 89
NPQTGEEIKI A+K+PAFKAGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1801HTHFIS290.019 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.019
Identities = 13/28 (46%), Positives = 16/28 (57%)

Query: 42 TLAIVGEAGSGKSTLARILVGAEPRSGG 69
TL I GE+G+GK +AR L R G
Sbjct: 162 TLMITGESGTGKELVARALHDYGKRRNG 189


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1802HTHFIS310.009 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.009
Identities = 9/16 (56%), Positives = 14/16 (87%)

Query: 38 LVGESGSGRSLLARAI 53
+ GESG+G+ L+ARA+
Sbjct: 165 ITGESGTGKELVARAL 180


66SO_1915SO_1925N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_1915-114-0.362742extracellular serine protease subtilase family
SO_1916-114-0.186753DMSO-responsive transcriptional activator of
SO_1917-2110.004806major facilitator superfamily transporter
SO_1918-110-0.414973multidrug and toxin efflux protein MATE family
SO_1921010-0.642882protein of unknown function DUF2986
SO_1922010-0.653470putative RNA-binding protein YqfB
SO_1923010-0.508188RND superfamily efflux pump permease component
SO_19242170.210142RND superfamily efflux pump permease component
SO_1925426-0.432118RND superfamily efflux pump MFP component
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1915SUBTILISIN992e-24 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 99.1 bits (247), Expect = 2e-24
Identities = 54/284 (19%), Positives = 89/284 (31%), Gaps = 84/284 (29%)

Query: 194 LIGSPKIWDGSATGTKAMGEGVIVGIIDSGINSDHASFADIGGDGYDHSNPLGQGIYIGD 253
+I +P +W+ + G GV V ++D+G ++DH
Sbjct: 28 MIQAPAVWNQTR------GRGVKVAVLDTGCDADHPDLKA-------------------- 61

Query: 254 CKTDFTSMCNDKLIGVRSYSEITNNYDDAKVFGDKPPAKNGEDYGGHGSHVASTAAGNIL 313
++IG R++++ GD K DY GHG+HVA T A
Sbjct: 62 -----------RIIGGRNFTDDDE--------GDPEIFK---DYNGHGTHVAGTIAAT-- 97

Query: 314 LNVPYVQGEAGKLEAEGIPTDIKFAQISGVAPHANIIAYQICNPGNAGDTYSGCPTAPIL 373
GVAP A+++ ++ N Y I+
Sbjct: 98 --------------ENENGV-------VGVAPEADLLIIKVLN-KQGSGQYDW-----II 130

Query: 374 KAIDDSIKDGVDVLNFSISGGGNPWNSATEQGFLAARNAGIFTAVAAGNTRPATATSAAI 433
+ I +I+ VD+++ S+ G + + A + I AAGN +
Sbjct: 131 QGIYYAIEQKVDIISMSLGGPED--VPELHEAVKKAVASQILVMCAAGNEGDGDDRTD-- 186

Query: 434 TQTPYSTPKNAPWYTSVANSTHDRDIVSAVEFNGKNYSFTAGSG 477
P SV DR N + G
Sbjct: 187 ---ELGYPGCYNEVISVGAINFDRHASEFSNSNNEVDLVAPGED 227



Score = 64.5 bits (157), Expect = 8e-13
Identities = 28/112 (25%), Positives = 42/112 (37%), Gaps = 27/112 (24%)

Query: 622 VAAPGTDIYAAYADQQFGHDKTGTDPADFTLMSGTSMASPHVAGAGALLKSL-----HKD 676
+ APG DI + + SGTSMA+PHVAGA AL+K L +D
Sbjct: 221 LVAPGEDILSTVPGG------------KYATFSGTSMATPHVAGALALIKQLANASFERD 268

Query: 677 WTPDQIRSALMLTATTAQAMKKADAKTIADPFDVGAGRIRVDLAAKTGLVMD 728
T ++ + L+ P G G + + + + D
Sbjct: 269 LTEPELYAQLIKRTI----------PLGNSPKMEGNGLLYLTAVEELSRIFD 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1917TCRTETB613e-12 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 61.0 bits (148), Expect = 3e-12
Identities = 75/392 (19%), Positives = 148/392 (37%), Gaps = 37/392 (9%)

Query: 14 RDTRLMWALCVASVVVYINLYLMQGMLPLLAEHFSVPGSQATLILSVTSFSLAFSLLIYA 73
R +++ LC+ S +N ++ LP +A F+ P + + + + + +Y
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 74 VVSDRIGRHTPIVVSLWLLALSNLL-LIWVEDFNALVYVRFLQGILLAAVPAIAMAYFKE 132
+SD++G ++ + + +++ + F+ L+ RF+QG AA PA+ M
Sbjct: 71 KLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVAR 130

Query: 133 QLSPSTMLKAAGIYIMANSIGGIAGRLLGGVMSQFLSWQESMWLLFLVTLAGVALTNYLL 192
+ KA G+ ++G G +GG+++ ++ W + L+ ++T+ V LL
Sbjct: 131 YIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWS-YLLLIPMITIITVPFLMKLL 189

Query: 193 PSGADAK---------VMSGG------QTSSPSRSKRARLLQDFYGFSHHL---TDP--Q 232
K +MS G T+S S S + F F H+ TDP
Sbjct: 190 KKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVD 249

Query: 233 MRL--------AYAIGGITFMMMVNQFSFIQLHLMAAPYEWSRFQA--TLIFLCYSSGTV 282
L GGI F + S + +M ++ S + +IF S +
Sbjct: 250 PGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY-MMKDVHQLSTAEIGSVIIFPGTMSVII 308

Query: 283 ASYFTAKWLAKFGQHKLYQWSWCLMLLGSL---LTLFDTTLTICMGFLMTACGFFLTHSC 339
Y + + G + + + L L T+ + + + G T +
Sbjct: 309 FGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTV 368

Query: 340 CNSFVAMRAS-RDRAKATSLYLCCYYLGAALG 370
++ V+ ++ SL +L G
Sbjct: 369 ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTG 400


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1923ACRIFLAVINRP500e-162 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 500 bits (1288), Expect = e-162
Identities = 214/1049 (20%), Positives = 446/1049 (42%), Gaps = 75/1049 (7%)

Query: 3 LTRLAIKLPVTTSMFFFAILLFGLASSRLLPLEMFPGIDIPQIVVQVPYKGSTPAEVERD 62
+ I+ P+ + +++ G + LP+ +P I P + V Y G+ V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 ITKVLEESLATMGGIDELESESSQEG-AEIEINMKWGENVATKSLEAREKIDAVRHLLPR 121
+T+V+E+++ + + + S S G I + + G + ++ + K+ LLP+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 DVERVFIRQFSTADMPVLTIRISSDRELSGAFDLLD---KQLKRPLERVEGVSKVSLYGV 178
+V++ I ++ ++ SD + D+ D +K L R+ GV V L+G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 179 EQKQIEVRINANRLAASGFSATELQARLARENFVLSAGTL------RESNLVYQVSPKGE 232
Q + + ++A+ L + ++ +L +N ++AG L L + +
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 233 FRNLEDIKALVLVPGLT-----LGDVADVQFSLPERSEGRHLDQHYAVGLDVFKESGANL 287
F+N E+ + L L DVA V+ + ++ A GL + +GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 288 VEVSDRVLKVIEQAKQDQQFQGIRLFIMEDQASGVKSSLTDLLLSGLVGALLSFIILYLF 347
++ + + + + + QG+++ D V+ S+ +++ + +L F+++YLF
Sbjct: 300 LDTAKAIKAKLAELQPFFP-QGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLF 358

Query: 348 LRNFKMTMIVVSSVPISIGMTLAAMYLLGYSLNILSMMGLLLAVGMLIDNAVVVTESVLQ 407
L+N + T+I +VP+ + T A + GYS+N L+M G++LA+G+L+D+A+VV E+V +
Sbjct: 359 LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVER 418

Query: 408 EKQGNSVNSNPQDNENAVMTGVDKVSLAVLAGTLTTAIVFLPNIFGVKVQLTIFLEHVAI 467
+ P++ A + ++ A++ + + VF+P + +I
Sbjct: 419 VMMED--KLPPKE---ATEKSMSQIQGALVGIAMVLSAVFIP-MAFFGGSTGAIYRQFSI 472

Query: 468 AICISLAASLLVAKTLIPLMLTKFHFDIEPDNTTGK-------------LQNFYNRSLNW 514
I ++A S+LVA L P + + ++ K N Y S+
Sbjct: 473 TIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGK 532

Query: 515 VLIRPWRSGLISIAILASTALPISMVKQDQEDSQSKERIYINYQVEGRHNLNVTEAMVNQ 574
+L R LI I+A + + + + Q+ T+ +++Q
Sbjct: 533 ILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQ 592

Query: 575 MEDYLYKNKEQ--FHIDTVYSY----YAPDDASSVILLKKDLPMPLDELKQKIRSGFP-- 626
+ DY KN++ + TV + A + + + LK P +E S
Sbjct: 593 VTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLK-----PWEERNGDENSAEAVI 647

Query: 627 ---KYSIAKPQFGWGDDNSGVRVTLTGRST--------------SELIHLSEQVLPLL-K 668
K + K + G+ + + G +T L Q+L + +
Sbjct: 648 HRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQ 707

Query: 669 NIKGLVDVRSEVNGAQQEVVIRINRQMAARLDLKLNEVASSISMALRGTPLRSFRHDPSG 728
+ LV VR + + ++++ A L + L+++ +IS AL GT + F D
Sbjct: 708 HPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFI-DRGR 766

Query: 729 ELRIEMAYEKEWQKSLDKLKQLPVVRIDQRLYTLDNLASIEIQPRFDTIKHYNRQTSLSI 788
++ + + +++ + + +L V + + + ++ YN S+ I
Sbjct: 767 VKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEI 826

Query: 789 GANLDN-LTTEEAQTKIKQVMENVSFPDGYNYSLRGGFERQEEDQSIMVINMLLAIAMIY 847
++ +A ++ + + P G Y G ++ + + ++ +++
Sbjct: 827 QGEAAPGTSSGDAMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVF 884

Query: 848 IVMAALFESLLLPTAIITSILFSITGVFWALLLTGTPMSVMAMIGILILMGVVVNNGIVL 907
+ +AAL+ES +P +++ + I GV A L V M+G+L +G+ N I++
Sbjct: 885 LCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILI 944

Query: 908 VDQINQ-MTPELDKLSDTIREVCITRLRPVLMTVGTTVLGLVPLAMDDTQIGGGGPPYYP 966
V+ M E + + RLRP+LMT +LG++PLA+ G G
Sbjct: 945 VEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAIS---NGAGSGAQNA 1001

Query: 967 MAIAIIGGLSFSTLTSLYLVPLCYQLLYR 995
+ I ++GG+ +TL +++ VP+ + ++ R
Sbjct: 1002 VGIGVMGGMVSATLLAIFFVPVFFVVIRR 1030


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1924ACRIFLAVINRP6620.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 662 bits (1709), Expect = 0.0
Identities = 257/1090 (23%), Positives = 471/1090 (43%), Gaps = 92/1090 (8%)

Query: 7 SVKRPVTVWMFMLAIMLFGMVGFSRLAVKLLPDLSYPTLTIRTMYDGAAPVEVEQLVSKP 66
++RP+ W+ + +M+ G + +L V P ++ P +++ Y GA V+ V++
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 67 IEEAVGVVKGLRKISSISRS-GMSDVVLEFEWGTTMDMASLDVREKLDTI--ALPLDVKK 123
IE+ + + L +SS S S G + L F+ GT D+A + V+ KL LP +V++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 124 PLLLRFNPNLDPIMRLALSVPNASEAELKQMRTYAEEELKRRLEALSGVAAVRLSGGLEQ 183
+ + +M N + Y +K L L+GV V+L G +
Sbjct: 125 QGISVEKSSSSYLMVAGFVSDNPGT-TQDDISDYVASNVKDTLSRLNGVGDVQLFGA-QY 182

Query: 184 EVHIQLNQEKLSQLNLNADDIKRRINEENINLSAGKVIQGD------REYLVRTLNQFNS 237
+ I L+ + L++ L D+ ++ +N ++AG++ + +F +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKN 242

Query: 238 LDELGQVIVYRDAQ-TLVRLFEVATITDAYKERSDITRIGSQESIELAIYKEGDANTVAV 296
+E G+V + ++ ++VRL +VA + + + I RI + + L I AN +
Sbjct: 243 PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDT 302

Query: 297 AKKLRDELVKINKD-PQQNKLEVIYDQSEFIESAVSEVTSSALMGSVLAMLVIYLFLRNI 355
AK ++ +L ++ PQ K+ YD + F++ ++ EV + +L LV+YLFL+N+
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 356 IPTLIISISIPFSVIATFNMMYFADISLNIMSLGGIALAIGLLVDNAIVVLENIDRC-RS 414
TLI +I++P ++ TF ++ S+N +++ G+ LAIGLLVD+AIVV+EN++R
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 415 EGMSKLDAAVTGTKEVAGAIFASTMTTLAVFVPLVFVDGIAGALFSDQALTVTFALLASL 474
+ + +A ++ GA+ M AVF+P+ F G GA++ ++T+ A+ S+
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 475 LVALTSIPMLASREGFKILPELMKKTPKEKPTTKLGKLKHYSATVFSFPIVLLFNYLPSA 534
LVAL P L + L+K E K
Sbjct: 483 LVALILTPALCAT--------LLKPVSAEHHENK-------------------------- 508

Query: 535 LLTFVLIIGRFFSWLLGLIMRPLSSGFNFVYHSTESIYHKLLAIALRKQLATLLLTTGIT 594
G FF W FN + + + Y + L LL+ I
Sbjct: 509 --------GGFFGW------------FNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIV 548

Query: 595 GACISLLPRLGMELIPPMNQGEFYVEILLPPGTAVGETDRILQQLALSI--KDRPEVKHA 652
+ L RL +P +QG F I LP G T ++L Q+ ++ V+
Sbjct: 549 AGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESV 608

Query: 653 YSQAGSGGLMTSDTARGGENWGRLQVELVDHSAYHQVTQVLRDTARRIPALEAKIEQPEL 712
++ G + +N G V L + R KI +
Sbjct: 609 FTVNGFS------FSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFV 662

Query: 713 FSFKTPLEIEL---TGYDLALLKRSADSLVNALSASDRFA-----------DINTSLRDG 758
F P +EL TG+D L+ ++ A ++ + + +
Sbjct: 663 IPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLED 722

Query: 759 QPELSIRFDHARLAALGMDAPTVANRIAQRVGGTVASQYTVRDRKIDILVRSQLDERDQI 818
+ + D + ALG+ + I+ +GGT + + R R + V++ R
Sbjct: 723 TAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLP 782

Query: 819 SDIDTLIINPDSSQPIALSAVAEVSLQLGPSAINRISQQRVALVSANLAYGDLSDAVADA 878
D+D L + + + + SA G + R + + A G S
Sbjct: 783 EDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMAL 842

Query: 879 QQILAAQVLPTSVQARFGGQNEEMEHSFQSLKIALILAVFLVYLVMASQFESLLHPLLIL 938
+ LA++ LP + + G + + S + ++ +V+L +A+ +ES P+ ++
Sbjct: 843 MENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVM 901

Query: 939 VAVPMALAGSVLGLYITQTHLSVVVFIGLIMLAGIVVNNAIVLVDRINQL-RSEGVEKLE 997
+ VP+ + G +L + V +GL+ G+ NAI++V+ L EG +E
Sbjct: 902 LVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVE 961

Query: 998 AIRVAAKSRLRPIMMTTLTTVLGLLPMALGLGDGAEVRAPMAITVIFGLSLSTLLTLIVI 1057
A +A + RLRPI+MT+L +LG+LP+A+ G G+ + + I V+ G+ +TLL + +
Sbjct: 962 ATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFV 1021

Query: 1058 PVLYAMFDRK 1067
PV + + R
Sbjct: 1022 PVFFVVIRRC 1031



Score = 114 bits (286), Expect = 1e-27
Identities = 95/520 (18%), Positives = 195/520 (37%), Gaps = 38/520 (7%)

Query: 578 IALRKQLATLLLTTGITGACISLLPRLGMELIPPMNQGEFYVEILLPPGTAVGETDRILQ 637
+R+ + +L + A + +L + P + V P A D + Q
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 638 QLALSIKDRPEVKHAYSQAGSGGLMTSDTARGGENWGRLQVELVDHSAYHQVTQVLRDTA 697
+ ++ + + S + S G +T L + QV
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTIT----------LTFQS-GTDPDIAQVQVQNKLQ 112

Query: 698 RRIPALEAKIEQPELFSFKTP----LEIELTGYDLALLKRS-----ADSLVNALSASDRF 748
P L +++Q + K+ + + + A ++ + LS +
Sbjct: 113 LATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGV 172

Query: 749 ADINTSLRDGQPELSIRFDHARLAALGMDAPTVANRI----AQRVGGTVASQYTVRDRKI 804
D+ Q + I D L + V N++ Q G + + +++
Sbjct: 173 GDVQLF--GAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQL 230

Query: 805 D--ILVRSQLDERDQISDIDTLIINPDSSQPIALSAVAEVSLQLGPSAIN-RISQQRVAL 861
+ I+ +++ ++ + TL +N D S + L VA V L + RI+ + A
Sbjct: 231 NASIIAQTRFKNPEEFGKV-TLRVNSDGS-VVRLKDVARVELGGENYNVIARINGKPAAG 288

Query: 862 VSANLAYGDLSDAVADA-QQILA--AQVLPTSVQA-RFGGQNEEMEHSFQSLKIALILAV 917
+ LA G + A A + LA P ++ ++ S + L A+
Sbjct: 289 LGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAI 348

Query: 918 FLVYLVMASQFESLLHPLLILVAVPMALAGSVLGLYITQTHLSVVVFIGLIMLAGIVVNN 977
LV+LVM +++ L+ +AVP+ L G+ L ++ + G+++ G++V++
Sbjct: 349 MLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDD 408

Query: 978 AIVLVDRINQ-LRSEGVEKLEAIRVAAKSRLRPIMMTTLTTVLGLLPMALGLGDGAEVRA 1036
AIV+V+ + + + + + EA + ++ + +PMA G +
Sbjct: 409 AIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYR 468

Query: 1037 PMAITVIFGLSLSTLLTLIVIPVLYAMF--DRKKFDHTNI 1074
+IT++ ++LS L+ LI+ P L A H N
Sbjct: 469 QFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_1925RTXTOXIND476e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 47.1 bits (112), Expect = 6e-08
Identities = 30/195 (15%), Positives = 65/195 (33%), Gaps = 15/195 (7%)

Query: 89 QSLAIIDAKRQQYDLDRSEAEVKIIEQELNRLKKMNNKEFISADSMAKLEYNLQAAIARR 148
+ + Y + E +I+ + + D + + N+
Sbjct: 259 NKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318

Query: 149 DLAELQVKESHVVSPIDGIIAKRYVKAGNMAKEFGD-LFYIV-NQDELHGIVHLPEQQLT 206
E + + S + +P+ + + V + L IV D L + + +
Sbjct: 319 AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIG 378

Query: 207 SLRLGQEAQV-FS--NQQSKNAINAKVLRISP--VVDPQSGT-FKVTLAVP-------NQ 253
+ +GQ A + + KV I+ + D + G F V +++ N+
Sbjct: 379 FINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNK 438

Query: 254 DARLKAGMFTRVELK 268
+ L +GM E+K
Sbjct: 439 NIPLSSGMAVTAEIK 453



Score = 40.6 bits (95), Expect = 6e-06
Identities = 19/84 (22%), Positives = 34/84 (40%), Gaps = 9/84 (10%)

Query: 37 PVETTTVIQGNVSSFYSTTATLEAPQEANVVSRIAGLIEVINVEEGDRVKKGQSLAIIDA 96
VE G ++ S + P E ++V I V+EG+ V+KG L + A
Sbjct: 79 QVEIVATANGKLTH--SGRSKEIKPIENSIVKEII-------VKEGESVRKGDVLLKLTA 129

Query: 97 KRQQYDLDRSEAEVKIIEQELNRL 120
+ D ++++ + E R
Sbjct: 130 LGAEADTLKTQSSLLQARLEQTRY 153


67SO_2081SO_2088N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_20810100.001373ATP-dependent helicase DinG family
SO_2082111-0.744485rod shape-determining-related protein
SO_2083112-0.395877chemotaxis signal transduction system methyl
SO_20851140.173246phenylalanyl-tRNA synthetase alpha subunit PheS
SO_2086-1120.977969phenylalanyl-tRNA synthetase beta subunit PheT
SO_20870161.292398integration host factor alpha subunit IhfA
SO_20880171.237481KDO 2-(lauroyl)-lipid IVA acyltransferase LpxM
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2081SECA310.019 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 31.0 bits (70), Expect = 0.019
Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 4/45 (8%)

Query: 29 QIISNAIASKGNAVIEAGTGVGKTFAYLIPAM---LSGKQVIVST 70
Q++ + ++ + E TG GKT +PA L+GK V V T
Sbjct: 87 QLLGGMVLNERC-IAEMRTGEGKTLTATLPAYLNALTGKGVHVVT 130


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2082SHAPEPROTEIN611e-13 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 60.5 bits (147), Expect = 1e-13
Identities = 49/145 (33%), Positives = 71/145 (48%), Gaps = 14/145 (9%)

Query: 1 MFTWLKGLISKDLLIELSEAKITINAFGDKASIEYEPLLALVTEHNKMMIK---AIGHEA 57
M +G+ S DL I+L A I G + + EP + + + K A+GH+A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKG-QGIVLNEPSVVAIRQDRAGSPKSVAAVGHDA 59

Query: 58 STLDG---EGIRIVNPFSHPRMFVASFALAEKLLQYGISQLHS-SGFRAAPRVILHQLEK 113
+ G I + P +A F + EK+LQ+ I Q+HS S R +PRV++
Sbjct: 60 KQMLGRTPGNIAAIRPMKDG--VIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLV----C 113

Query: 114 TEGGLTDIEDRVLRELAMGAGAREV 138
G T +E R +RE A GAGAREV
Sbjct: 114 VPVGATQVERRAIRESAQGAGAREV 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2087DNABINDINGHU1147e-37 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 114 bits (286), Expect = 7e-37
Identities = 33/89 (37%), Positives = 54/89 (60%)

Query: 4 TKAEMAEHLFETLGINKRVAKEMVESFFEEIRGALESGEQVKLSGFGNFDLRDKNQRPGR 63
K ++ + E + K+ + V++ F + L GE+V+L GFGNF++R++ R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 64 NPKTGEDIPISARRVVTFRPGQKLKTRVE 92
NP+TGE+I I A +V F+ G+ LK V+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAVK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2088BORPETOXINA290.019 Bordetella pertussis toxin A subunit signature.
		>BORPETOXINA#Bordetella pertussis toxin A subunit signature.

Length = 269

Score = 29.4 bits (65), Expect = 0.019
Identities = 13/27 (48%), Positives = 15/27 (55%)

Query: 22 WVTWLAIGLLVIFGIMPAWLRDPIAKV 48
W+TWLAI + PAW DP A V
Sbjct: 15 WLTWLAILAVTAPVTSPAWADDPPATV 41


68SO_2119SO_2127N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2119222-5.097546protein phosphatase with response regulator
SO_2120119-3.424137chemotaxis signal transduction system response
SO_2121118-2.925922chemotaxis signal transduction system histidine
SO_2122018-3.154094chemotaxis signal transduction system adaptor
SO_2123-118-3.239740chemotaxis signal transduction system methyl
SO_2124019-3.369902chemotaxis signal transduction system MCP
SO_2125116-2.943780chemotaxis signal transduction system
SO_2126115-2.478545chemotaxis signal transduction system response
SO_2127-112-0.944041diguanylate cyclase with response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2119HTHFIS639e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.3 bits (154), Expect = 9e-13
Identities = 26/122 (21%), Positives = 52/122 (42%)

Query: 11 ILIVDNDAIASQSISDFIHGKGYNVIICDNLEDAFFEVSLNKIDLILVNYFQPDGTALTL 70
IL+ D+DA ++ + GY+V I N + ++ DL++ + PD A L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 71 LAHLDSLSKEIPVVVINDKKEPQAFLDCFKMGVLDFIVKPINVEVFWYKAEILLTRIKLQ 130
L + ++PV+V++ + + + G D++ KP ++ L K +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 131 RK 132

Sbjct: 126 PS 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2120HTHFIS872e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 2e-23
Identities = 30/122 (24%), Positives = 56/122 (45%), Gaps = 3/122 (2%)

Query: 1 MSK-KILIVDDSAAIRQMVEATLKSANYQVTLAKDGREALDLCSSQRFDFILTDQNMPRM 59
M+ IL+ DD AAIR ++ L A Y V + + ++ D ++TD MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 DGLTLIKSLRGMSAFMRTPIIMLTTEAGDDMKAQGKAVGATGWMVKPFDPQKLLAITAKV 119
+ L+ ++ A P+++++ + + GA ++ KPFD +L+ I +
Sbjct: 61 NAFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 120 LG 121
L
Sbjct: 119 LA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2121PF06580358e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 35.2 bits (81), Expect = 8e-04
Identities = 13/66 (19%), Positives = 31/66 (46%), Gaps = 10/66 (15%)

Query: 418 EIDKGMIEKLVDPLT--HLVRNSLDHGIEKPEVRRLLGKAEIAQLSLRASQRGGNIVIAV 475
+I+ +++ V P+ LV N + HGI + + ++ L+ ++ G + + V
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQ--------LPQGGKILLKGTKDNGTVTLEV 296

Query: 476 HDDGAG 481
+ G+
Sbjct: 297 ENTGSL 302


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2126HTHFIS682e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.5 bits (165), Expect = 2e-14
Identities = 28/107 (26%), Positives = 47/107 (43%), Gaps = 5/107 (4%)

Query: 2 AIKVLVVDDSTLIRSLLGKMIESDPELSLVGMAADAYMAKDMVNQFRPDVITLDIEMPKV 61
+LV DD IR++L + + + ++A + D++ D+ MP
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRI--TSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 62 DGLTFLDRLMKARPTA-VVMISALTEEGADATFNALALGAVDFIPKP 107
+ L R+ KARP V+++SA A GA D++PKP
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNT--FMTAIKASEKGAYDYLPKP 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2127HTHFIS768e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 76.0 bits (187), Expect = 8e-17
Identities = 34/201 (16%), Positives = 69/201 (34%), Gaps = 19/201 (9%)

Query: 255 KVLLVDDQQSMVDYFSSLLRSHGLMVRGMTKPEQVIPTLEQFEPDLFIFDLYMPGVNGLE 314
+L+ DD ++ + L G VR + + + + DL + D+ MP N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 315 LARMIRQLDKYTSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP--SLFVTQVISRAQ 372
L I++ P+LV+S+ +T + + G+ D + K + +
Sbjct: 65 LLPRIKKARPDL--PVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 373 RGHDIRSSASRDSLTGLLNHTQILVAARHCINLAKRANSSVCIAMLDLDHFKQVNDTYGH 432
+ + L+ + + + + + ++ I G
Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMIT--------------GE 168

Query: 433 SGGDKVLLAFAHLLQQSLRAA 453
SG K L+A A L R
Sbjct: 169 SGTGKELVARA-LHDYGKRRN 188



Score = 46.7 bits (111), Expect = 2e-07
Identities = 32/135 (23%), Positives = 61/135 (45%), Gaps = 4/135 (2%)

Query: 132 IAIIEDDNNVGAMITKQLHEFGFNVQHFLNFTDFIKVQYASPFDLILLDLILPDYTEDAL 191
I + +DD + ++ + L G++V+ N + A DL++ D+++PD E+A
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPD--ENAF 63

Query: 192 -FEAACEFEKNNTRVFVLSSRSDFDMRLLAIRANVSEYFVKPAETTLLVRKIHQWLKMSD 250
+ + + V V+S+++ F + A +Y KP + T L+ I + L
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 251 KPPLKVLLVDDQQSM 265
+ P L D Q M
Sbjct: 124 RRP-SKLEDDSQDGM 137


69SO_2185SO_2194N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2185-1131.363573exopolyphosphatase Ppx
SO_21870131.806130ISSod11 transposase TnpA_ISSod11
SO_21890163.445467hsp70 family protein YegD
SO_2190-1173.293133lipoprotein CreA
SO_2191-2163.118876cystathionine beta-lyase MetC
SO_2192-3152.386131two component signal transduction system
SO_2193-3121.531876two component signal transduction system
SO_2194-2120.725196sortase system OmpA family protein PdsO
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2185SHAPEPROTEIN290.039 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 29.3 bits (66), Expect = 0.039
Identities = 16/36 (44%), Positives = 24/36 (66%)

Query: 137 NIVIDIGGGSTEVVLGQKNTPTHLSSLRCGCVSFNE 172
++V+DIGGG+TEV + N + SS+R G F+E
Sbjct: 161 SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDE 196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2189SHAPEPROTEIN423e-06 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 42.4 bits (100), Expect = 3e-06
Identities = 27/83 (32%), Positives = 43/83 (51%), Gaps = 15/83 (18%)

Query: 191 AAKRAGFVDVAFLFEPLAAGMDYEAGLKADQTV--LVVDVGGGTTDCSVVKMGPSHKASF 248
+A+ AG +V + EP+AA + AGL + +VVD+GGGTT+ +V+ +
Sbjct: 129 SAQGAGAREVFLIEEPMAAAIG--AGLPVSEATGSMVVDIGGGTTEVAVISLN------- 179

Query: 249 DRSRDCLGHSGQRIGGNDLDIAL 271
+ S RIGG+ D A+
Sbjct: 180 ----GVVYSSSVRIGGDRFDEAI 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2193HTHFIS639e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.9 bits (153), Expect = 9e-14
Identities = 27/130 (20%), Positives = 53/130 (40%), Gaps = 2/130 (1%)

Query: 3 RIAIVEDEAAIRENYKDVLQQHGYSVQTYADRPSAMLAFNTRLPDLAIIDIGLGNEIDGG 62
I + +D+AAIR L + GY V+ ++ + DL + D+ + +E
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE--NA 62

Query: 63 FMLCQSLRAMSNTLPIIFLTARDSDFDTVCGLRLGADDYLSKEVSFPHLTARLAALFRRS 122
F L ++ LP++ ++A+++ + GA DYL K L +
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 123 ELAASQTPQE 132
+ S+ +
Sbjct: 123 KRRPSKLEDD 132


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2194OMPADOMAIN616e-13 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 61.1 bits (148), Expect = 6e-13
Identities = 27/125 (21%), Positives = 52/125 (41%), Gaps = 11/125 (8%)

Query: 147 ELALGMNVQFRTGSSELEADFLPQLDNVATVMKRSSESN--LELKGYAERRGDWRDNQSL 204
L +V F + L+ + LD + + + + + + GY +R G NQ L
Sbjct: 214 HFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGL 273

Query: 205 SEHRLLEVRDYLIQQGVAPARMTTQALG-----AAMSLNEQLDSESDVS----DRRVTLT 255
SE R V DYLI +G+ +++ + +G + + + + DRRV +
Sbjct: 274 SERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIE 333

Query: 256 IPPTQ 260
+ +
Sbjct: 334 VKGIK 338


70SO_2537SO_2547N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2537-115-3.311042sodium:proton antiporter CPA1 family
SO_2538020-5.744344two component signal transduction system
SO_2539027-8.552542two component signal transduction system
SO_2540131-9.627803two component signal transduction system
SO_2541130-9.670411two component signal transduction system
SO_2542232-10.429789signaling protein with FIST domain
SO_2543335-11.700556two component signal transduction system
SO_2544434-11.469040two component signal transduction system hybrid
SO_2545123-7.327424two component signal transduction system
SO_2546121-4.467126chemotaxis signal transduction system inhibitor
SO_2547022-4.912725chemotaxis signal transduction system response
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2537IGASERPTASE373e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 37.0 bits (85), Expect = 3e-04
Identities = 20/77 (25%), Positives = 33/77 (42%), Gaps = 9/77 (11%)

Query: 594 ALEEAKIQQMIAEQEAIAAQTKAAEEATLAKAKAEEKAEVERQRLDQQAQM----KAKQS 649
+ ++ Q E QT +E A + EEKA+VE ++ + ++ KQ
Sbjct: 1079 NTQTNEVAQS--GSETKETQTTETKET--ATVEKEEKAKVETEKTQEVPKVTSQVSPKQE 1134

Query: 650 QSEHE-PQDAIDRSDET 665
QSE PQ R ++
Sbjct: 1135 QSETVQPQAEPARENDP 1151


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2538HTHFIS492e-08 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 49.1 bits (117), Expect = 2e-08
Identities = 32/155 (20%), Positives = 63/155 (40%), Gaps = 12/155 (7%)

Query: 4 VLFVDDDSFMLRALLRLAKRLRPEWQ-FWTEEDGLNWAKSIPHNVNIDLIVCDYLMPDIN 62
+L DDD+ + L + R + + W + DL+V D +MPD N
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGD----GDLVVTDVVMPDEN 61

Query: 63 GDSVLIEASKHFPLAIRALLTGDTTEEVVCKAGKA-AHFVLSKPFNEQDIVQLL-TCIER 120
+L K P +++ T KA + A+ L KPF+ +++ ++ +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121

Query: 121 IHKLPFTHEVRA-----MLGASALLLPLPDIVQRV 150
+ P E + ++G SA + + ++ R+
Sbjct: 122 PKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2539HTHFIS1024e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 102 bits (256), Expect = 4e-25
Identities = 39/134 (29%), Positives = 64/134 (47%), Gaps = 2/134 (1%)

Query: 1 MDK-SLLLVDDDVGILKALTRLLTRSGYSVKTAQSGEEALTLLLNYDCKVVLTDFRMPYM 59
M ++L+ DDD I L + L+R+GY V+ + + D +V+TD MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 60 DGGQLLSKIKRLYPDIVSLVISGYSDFESVKSLLNAGSAYRFLQKPWEDDELLGEIANAF 119
+ LL +IK+ PD+ LV+S + F + G AY +L KP++ EL+G I A
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKG-AYDYLPKPFDLTELIGIIGRAL 119

Query: 120 THYAKHLFQHQSQK 133
+ + +
Sbjct: 120 AEPKRRPSKLEDDS 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2540HTHFIS895e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.7 bits (220), Expect = 5e-23
Identities = 28/116 (24%), Positives = 58/116 (50%), Gaps = 3/116 (2%)

Query: 73 DKKSILIIDDELSMRNALRRALQSTPFTILTAQDGFQAGVKVIAEKPDLILLDLSLPGLD 132
+IL+ DD+ ++R L +AL + + + + A DL++ D+ +P +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 133 GFEVIQFIRQRPDLAKLKILVLSGLSSIELA-ESIRLGADDAIAKPFDNHDLLDRV 187
F+++ I++ L +LV+S ++ A ++ GA D + KPFD +L+ +
Sbjct: 62 AFDLLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGII 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2541HTHFIS886e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.0 bits (218), Expect = 6e-21
Identities = 31/159 (19%), Positives = 67/159 (42%), Gaps = 5/159 (3%)

Query: 12 ILCVDDEASILKSLQRLFIGKDLQILLADSGSKALELMLEHRVNVIITDMRMPNMTGAEF 71
IL DD+A+I L + + + + + + ++++TD+ MP+ +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 72 LAKAAILQPDAYRILMTGYADLASTVSAINLGKIHRYVQKPWDNQELLTVVDEGLALCHL 131
L + +PD ++M+ + + A G + Y+ KP+D EL+ ++ AL
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKG-AYDYLPKPFDLTELIGIIGR--ALAEP 122

Query: 132 IRQNKQLTAKVATQNKQLKELNSSLEETVLKRTEQLKQT 170
R+ +L + S+ + + + +L QT
Sbjct: 123 KRRPSKLEDDSQDGMPLVGR--SAAMQEIYRVLARLMQT 159


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2543PF06580364e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 35.6 bits (82), Expect = 4e-04
Identities = 31/180 (17%), Positives = 69/180 (38%), Gaps = 28/180 (15%)

Query: 283 LAGARRARDIIKNL-----RNFSHPDENTISTINILELITDTVRIANTQVKKHARIKINH 337
L +AR+++ +L + + + +S + L ++ +++A ++ R++ +
Sbjct: 187 LEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLA--SIQFEDRLQFEN 244

Query: 338 DLHHAFTQGNATQLSQVILNLINNA-HHSIKH--QHGLIEISINKFNNWINIEIEDNGCG 394
++ A + ++ L+ N H I Q G I + K N + +E+E+ G
Sbjct: 245 QINPAI--MDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSL 302

Query: 395 IDDTDIPHIFEPFFTTKEIGQGTGLGLSISRAIIEQHNGCIALVHTGLK--GTKFVISLP 452
K + TG GL R ++ G A + K ++ +P
Sbjct: 303 A--------------LKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2544HTHFIS672e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.2 bits (164), Expect = 2e-13
Identities = 26/112 (23%), Positives = 45/112 (40%), Gaps = 2/112 (1%)

Query: 807 HVLIVDDVEDIRELIDIYLKDTEIAVDFAQNGQQAIQLVEKSHYDLVILDQQMPIMDGFT 866
+L+ DD IR +++ L V N + + DLV+ D MP + F
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 867 AAKAIREFNKSIPLLLLSA--DILDTEPHQKSPFNKTIAKPFTKNQLIETIR 916
I++ +P+L++SA + + + KPF +LI I
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2545PF06580290.038 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.1 bits (65), Expect = 0.038
Identities = 19/96 (19%), Positives = 34/96 (35%), Gaps = 17/96 (17%)

Query: 327 NLLVNAAQAIEERGEISIDVSASDAEFIIVIRDTGSGIAASDLRKIFEPFYTTKLVGTGT 386
N + + + + G+I + + + + + +TGS K T
Sbjct: 266 NGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLAL--------------KNTKEST 311

Query: 387 GLGLSLSYSIVQKHKGE---IKVSSVLGEGTAFTVI 419
G GL +Q G IK+S G+ A +I
Sbjct: 312 GTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2547HTHFIS613e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 61.4 bits (149), Expect = 3e-14
Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 5/112 (4%)

Query: 5 VTIADDSLMSRKAVRRALPEDWDVEITEACNGKEALEAANSGKAEVLFLDLTMPELDGFG 64
+ +ADD R + +AL ++ N +G +++ D+ MP+ + F
Sbjct: 6 ILVADDDAAIRTVLNQAL-SRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 65 VLKYLHEQQSKTVVIVISADIQPEAKLLVDSL--GAFRFLQKPLQPAQLREA 114
+L + + + V+V+SA Q + + GA+ +L KP +L
Sbjct: 65 LLPRIKKARPDLPVLVMSA--QNTFMTAIKASEKGAYDYLPKPFDLTELIGI 114


71SO_2813SO_2832N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_2813015-3.458583oxidoreductase short chain
SO_2815016-4.095555transporter-like protein HCC family
SO_2817-116-3.425728ISSod4 transposase TnpA_ISSod4
SO_2819-118-3.152866ISSod11 transposase TnpA_ISSod11
SO_2821-118-2.050343probable metal-binding protein YecH
SO_2822-116-2.256174two component signal transduction system
SO_2823-116-2.394965two component signal transduction system
SO_2827-219-1.985258protein of unknown function DUF2132
SO_2829-113-0.745688hypothetical protein
SO_2830-114-0.606915predicted outer membrane protein
SO_28310130.340204GTP cyclohydrolase II RibA
SO_28320120.419119protein of unknown function DUF2810
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2813DHBDHDRGNASE1299e-39 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 129 bits (326), Expect = 9e-39
Identities = 85/257 (33%), Positives = 130/257 (50%), Gaps = 15/257 (5%)

Query: 3 SSNNLQGKVAFVQGGSRGIGAAIVKRLASEGAAVAFTYVSSEAQSQLLVDEVIAQGGKAI 62
++ ++GK+AF+ G ++GIG A+ + LAS+GA +A + E + +V + A+ A
Sbjct: 2 NAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKL-EKVVSSLKAEARHAE 60

Query: 63 AIKADSTEPEAIRRAIRETKAHLGGLDIVVNNAGILIWDSIENLTLEDWERIVNTNVRSV 122
A AD + AI + +G +DI+VN AG+L I +L+ E+WE + N V
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 123 FVASQEAALHMND--GGRIINIGSTNAERIPFVGGAIYGMSKSALVGLAKGLARDLGPRA 180
F AS+ + +M D G I+ +GS N +P A Y SK+A V K L +L
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGS-NPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYN 179

Query: 181 ITVNNIQPGPVDTDMN-----PDNGD------SSEPIKAIGVLGRYGKAEEIASFVAFIA 229
I N + PG +TDM +NG S E K L + K +IA V F+
Sbjct: 180 IRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

Query: 230 GPEAGYITGASLMIDGG 246
+AG+IT +L +DGG
Sbjct: 240 SGQAGHITMHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2822PF065802283e-72 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 228 bits (584), Expect = 3e-72
Identities = 62/198 (31%), Positives = 114/198 (57%), Gaps = 3/198 (1%)

Query: 357 EQQQNLLTQAELKLLQAQVNPHFLFNALNTISAIIKRDPDMSKQLLQQLSQFLRINLKRT 416
+ ++ +A+L L+AQ+NPHF+FNALN I A+I DP ++++L LS+ +R +L+ +
Sbjct: 152 WKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKAREMLTSLSELMRYSLRYS 211

Query: 417 TG-LVTLGDELDHIASYLTIEKARFINKLQVNIAIPESLYHCKVPAFTLQPIIENAVKHG 475
V+L DEL + SYL + +F ++LQ I ++ +VP +Q ++EN +KHG
Sbjct: 212 NARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHG 271

Query: 476 TSHMIDQGQIKVTGRLDEHVLALEVIDNAGLYQPST-DSEGLGMNLVHKRIQNLFGEQYG 534
+ + G+I + G D + LEV + L +T +S G G+ V +R+Q L+G +
Sbjct: 272 IAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQNVRERLQMLYGTEAQ 331

Query: 535 LQVECEPDEYTKVIIRLP 552
+++ + + ++ +P
Sbjct: 332 IKLSEKQGKVN-AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2823HTHFIS682e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 67.9 bits (166), Expect = 2e-15
Identities = 33/115 (28%), Positives = 53/115 (46%), Gaps = 9/115 (7%)

Query: 5 LIVDDELFAREELADSLSQEADIEIIGQCSNAIEALQTITKEKPQLVFLDIQMPRISGME 64
L+ DD+ R L +LS+ + SNA + I LV D+ MP + +
Sbjct: 7 LVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 65 LIAML---DPDTLPKIVFVTAFDEF--AVKAFDNHAFDYLLKPIDADRLSKTLKR 114
L+ + PD ++ ++A + F A+KA + A+DYL KP D L + R
Sbjct: 65 LLPRIKKARPDL--PVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2827PF05616260.049 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 25.9 bits (56), Expect = 0.049
Identities = 14/47 (29%), Positives = 19/47 (40%), Gaps = 2/47 (4%)

Query: 69 NKLPLPAAKSIQAPAKEPSKKSAP--KPNSKPTQTKVVTPTPAVEGQ 113
N PLP + PA P+ P +PN +P P +GQ
Sbjct: 324 NAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTDGQ 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_2832OMADHESIN260.031 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

Length = 455

Score = 26.4 bits (57), Expect = 0.031
Identities = 14/38 (36%), Positives = 21/38 (55%)

Query: 11 NDKLDKFRRKLAAAEQRGDVVIVAQFKREIEAVTKQIN 48
++ L++ LAA + D V VAQ K+EIE + N
Sbjct: 189 HESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTN 226


72SO_3196SO_3252N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3196019-3.665396two component signal transduction system
SO_3197-122-3.638467phospholipid transport-associated protein MlaA
SO_3198022-3.297893putative cytoplasmic protein in chemotaxis
SO_3199220-2.425386FlhB domain protein
SO_3200225-1.718257putative membrane anchored protein of unknown
SO_3202321-0.976831chemotaxis signal transduction system adaptor
SO_3203321-1.083313chemotaxis signal transduction system adaptor
SO_3204220-0.961289ParA family protein
SO_3205120-1.154800putative membrane anchored protein in chemotaxis
SO_3206120-1.308399chemotaxis signal transduction system response
SO_3207117-1.263349chemotaxis signal transduction system histidine
SO_3208016-1.587087chemotaxis signal transduction system CheY
SO_3209-215-1.017139chemotaxis signal transduction system response
SO_3210-215-0.850715RNA polymerase sigma-28 factor for flagellar
SO_3211-315-0.654326flagellar polar localization control system FlhF
SO_3212-214-0.848014flagellar polar localization control system
SO_3213-116-1.046746flagellar export protein FlhA
SO_3215-118-1.003792flagellar export protein FlhB
SO_3216017-1.182002flagellar export protein FliR
SO_3217016-0.822496flagellar export protein FliQ
SO_3218014-0.944578flagellar export protein FliP
SO_3219115-0.726359flagellar export protein FliO
SO_32200130.853571flagellar motor switching and energizing
SO_32210120.741111flagellar motor switching and energizing
SO_32221120.534618flagellar basal body protein FliL
SO_32230130.832644flagellar hook-length control protein FliK
SO_3224-1121.218384flagellar chaperone escort protein FliJ
SO_3225-2121.360246flagellar protein export ATPase FliI
SO_3226-1100.235286flagellar assembly protein FliH
SO_3227-1120.204750flagellar motor switching and energizing
SO_3228-113-0.045871flagellar basal-body MS-ring and collar protein
SO_3229016-1.103012flagellar basal-body component FliE
SO_3230018-1.834466two component signal transduction system
SO_3231321-2.736635two component signal transduction system
SO_3232220-3.309243Sigma54-dependent transcriptional regulator for
SO_3233427-4.849915chaperone for FliC flagellin FliS
SO_3234424-3.989808flagella biosynthesis chaperone for FliD FliT
SO_3235220-2.693007flagellar filament capping protein FliD
SO_3236118-1.685387uncharacterized flagella locus protein FlaG
SO_3237016-1.150666flagellin FliC
SO_3238-114-0.772646flagellin FliC
SO_3239-212-0.184921flagellar hook-associated protein FlgL
SO_3240-2130.521389flagellar hook-filament junction protein FlgK
SO_32410150.418082flagellar rod cap protein and peptidoglycan
SO_3242116-0.063399flagellar P-ring protein FlgI
SO_3243118-0.487437flagellar L-ring protein FlgH
SO_3244219-0.919513flagellar component of cell-distal portion of
SO_3245218-1.618188flagellar component of cell-proximal portion of
SO_3247118-2.832775flagellar hook protein FlgE
SO_3248-120-4.214846flagellar hook assembly protein FlgD
SO_3249-120-4.399000flagellar basal-body rod protein FlgC
SO_3250122-4.698685flagellar basal-body rod protein FlgB
SO_3251122-4.453690chemotaxis signal transduction system MCP
SO_3252122-3.976461chemotaxis signal transduction system response
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3196HTHFIS944e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 93.7 bits (233), Expect = 4e-23
Identities = 36/154 (23%), Positives = 62/154 (40%), Gaps = 2/154 (1%)

Query: 7 SILWVEDDPVFRQIVATFLSGRGAQVVQAGDGEQGLIHFKQQRFDIILADLSMPKLGGLD 66
+IL +DD R ++ LS G V + D+++ D+ MP D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 67 MLKEMSKLEPLVPSIVISGNNVMADVVEALRIGACDYLVKPVADLFIIEQAIQQGLQRHQ 126
+L + K P +P +V+S N ++A GA DYL KP DL + I + L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF-DLTELIGIIGRALAEPK 123

Query: 127 LDDISQTDLDVLSHQELSDNLTILEQSVEAAKQV 160
S+ + D L +++ ++
Sbjct: 124 R-RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3197VACJLIPOPROT2292e-77 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 229 bits (584), Expect = 2e-77
Identities = 88/224 (39%), Positives = 128/224 (57%), Gaps = 4/224 (1%)

Query: 42 EDPRDPFEGFNRVMWDFNYLYLDRYLYRPVAHGYNDYIPLPAKMGVNNFLQNLEEPSSVV 101
+ DP EGFNR M++FN+ LD Y+ RPVA + DY+P PA+ G++NF NLEEP+ +V
Sbjct: 26 QGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMV 85

Query: 102 NNLLQGKWGWAANAGGRFTVNTTIGLLGVIDVADMMGMTRKQDE---FNEVLGYYGVPNG 158
N LQG RF +NT +G+ G IDVA M ++ E F LG+YGV G
Sbjct: 86 NYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYG 145

Query: 159 PYFMAPFAGPYIVRELASDWVDGLYFPLSELTMWQSVLKWGLKSLHARASAIDQERLVDN 218
PY PF G + +R+ D D LY LS LT SV KW L+ + RA +D + L+
Sbjct: 146 PYVQLPFYGSFTLRDDGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQ 205

Query: 219 ALDPYAFVKDAYIQHMDYKVYDGNV-PQKQEDDELLDQYMQELE 261
+ DPY V++AY Q D+ G + PQ+ + + + +++++
Sbjct: 206 SSDPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQDDLKDID 249


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3199TYPE3IMSPROT561e-12 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 55.5 bits (134), Expect = 1e-12
Identities = 18/87 (20%), Positives = 32/87 (36%), Gaps = 3/87 (3%)

Query: 7 TQQAVALSYD-GKH-APKVVASGEGLVADEIIALAKASGVYIHQDPHLSNFL-RLLELGE 63
T A+ + Y G+ P V + +A+ GV I Q L+ L +
Sbjct: 265 THIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDH 324

Query: 64 EIPRELYLLIAELIAFVYMLDGKFPEQ 90
IP E AE++ ++ + +
Sbjct: 325 YIPAEQIEATAEVLRWLERQNIEKQHS 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3203PF03544290.018 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 29.2 bits (65), Expect = 0.018
Identities = 16/88 (18%), Positives = 28/88 (31%)

Query: 79 KSIVTVSTKENAEPLVNKQALERLLAPVLKTQAPDIPKPTELNEQPLPLPKPVEAIAVTN 138
S+ V+ + P + E ++ P + + P P PKP
Sbjct: 50 ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVK 109

Query: 139 VVKPADVESQQVEIISTAPETQVGFAPP 166
V+ + + VE +P A P
Sbjct: 110 KVEQPKRDVKPVESRPASPFENTAPARP 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3206HTHFIS651e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.9 bits (158), Expect = 1e-13
Identities = 28/135 (20%), Positives = 56/135 (41%), Gaps = 7/135 (5%)

Query: 2 AIKVLVVDDSSFFRRRVSEIVNQDPELEVIATASNGAEAVKMTADLNPQVITMDIEMPVM 61
+LV DD + R +++ +++ + SN A + A + ++ D+ MP
Sbjct: 3 GATILVADDDAAIRTVLNQALSR--AGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 62 DGITAVREIMAKCP-TPILMFSSLTHDGAKATLDALDAGALDFLPKRF--EDIATNKDEA 118
+ + I P P+L+ S+ + + A + GA D+LPK F ++ A
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSA--QNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 119 ILLLQQRVKALGRRR 133
+ ++R L
Sbjct: 119 LAEPKRRPSKLEDDS 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3207PF06580432e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 43.3 bits (102), Expect = 2e-06
Identities = 19/105 (18%), Positives = 37/105 (35%), Gaps = 23/105 (21%)

Query: 450 TLNKEIDLVLV---------GEETDLDKNLVEALADPLVH------LVRNSVDHGIEMPN 494
+L E+ +V + + + A+ D V LV N + HGI
Sbjct: 217 SLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGIA--- 273

Query: 495 DREASGKPRTGVITLSASQEGDHILLKIEDDGAGMDPEKLKQIAI 539
P+ G I L +++ + L++E+ G+ +
Sbjct: 274 -----QLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGT 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3209HTHFIS903e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.9 bits (223), Expect = 3e-24
Identities = 33/105 (31%), Positives = 51/105 (48%), Gaps = 3/105 (2%)

Query: 6 KILIVDDFSTMRRIIKNLLRDLGFNNTQEADDGSTALPMLQKGDFDFVVTDWNMPGMQGI 65
IL+ DD + +R ++ L G++ + +T + GD D VVTD MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 DLLKAIRADDSLKHLPVLMVTAEAKREQIIAAAQAGVNGYVVKPF 110
DLL I+ LPVL+++A+ I A++ G Y+ KPF
Sbjct: 64 DLLPRIKKAR--PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3212PF05272310.012 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.012
Identities = 9/25 (36%), Positives = 12/25 (48%)

Query: 238 VKQGGVVALVGPTGVGKTTSLAKLA 262
K V L G G+GK+T + L
Sbjct: 593 CKFDYSVVLEGTGGIGKSTLINTLV 617


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3213HTHFIS310.013 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.3 bits (71), Expect = 0.013
Identities = 26/158 (16%), Positives = 52/158 (32%), Gaps = 19/158 (12%)

Query: 485 VVDAATVVATHISQILTNNAAKLLGYEEAQQLMDMLAKHSPKLVDGFIPDV-MPLGNVVK 543
V D + T ++Q L+ + A L +A LV + DV MP N
Sbjct: 8 VADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLV---VTDVVMPDENAFD 64

Query: 544 VMQNLLNEGVSVR--------DLRTIVQTL----LEYGTKSNDTEVLTAAVRIAL---KR 588
++ + + T ++ +Y K D L + AL KR
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 589 MIVQEISGPELEIPVITLAPELEQMLHQSMQATGGDGP 626
+ + +P++ + ++++ + D
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLT 162


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3215TYPE3IMSPROT335e-116 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 335 bits (861), Expect = e-116
Identities = 96/347 (27%), Positives = 178/347 (51%), Gaps = 2/347 (0%)

Query: 6 SGERSEEPTGRRLDQAREKGQVARSKELGTATVLLSAATGLYMLGPGIAKALSNVFERVF 65
SGE++E+PT +++ AR+KGQVA+SKE+ + ++++ + L L + S + +
Sbjct: 2 SGEKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLM--LI 59

Query: 66 TMDRAAIFDTNQMFNVWGVVGSEIGWPLLKIMLLIVVVAFIGNVSLGGMNFSTQAMMPKA 125
+++ + + + V V E + ++ + ++A +V G S +A+ P
Sbjct: 60 PAEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDI 119

Query: 126 SKMSPIAGFKRMFGVQALVELTKGIAKFSVVALASYLLLSHYFNDILLLSADHLPGNVYH 185
K++PI G KR+F +++LVE K I K ++++ ++++ +L L +
Sbjct: 120 KKINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPL 179

Query: 186 ALDLLVWMFILLCSSVLVIVVIDVPFQIWNHNKQLKMTKQEVKDEYKDTEGKPEVKGRVR 245
+L + ++ +VI + D F+ + + K+LKM+K E+K EYK+ EG PE+K + R
Sbjct: 180 LGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRR 239

Query: 246 QMQRELAQRRMMAEVPNADVIVVNPEHYAVAIKYDVKRSAAPFVIAKGVDEVAFKIREVA 305
Q +E+ R M V + V+V NP H A+ I Y + P V K D +R++A
Sbjct: 240 QFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIA 299

Query: 306 RAHNIAIVTAPPLARAIYHTTKLDQQVPEGLFTAVAQVLAYVFQLRQ 352
+ I+ PLARA+Y +D +P A A+VL ++ +
Sbjct: 300 EEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQNI 346


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3216TYPE3IMRPROT1198e-35 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 119 bits (301), Expect = 8e-35
Identities = 92/243 (37%), Positives = 141/243 (58%), Gaps = 1/243 (0%)

Query: 15 YMWPLFRVASMLMVMVVFGAATTPTRVRLLLAIAITLAIAPVLPPVKDAQLFSLSAVFIT 74
Y WPL RV +++ + + P RV+L LA+ IT AIAP LP +FS A+++
Sbjct: 16 YFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPANDVP-VFSFFALWLA 74

Query: 75 AQQIIIGVAMGFVTQMVMQTFVLTGQIIGMQTSLGFASMVDPSSGQQTPVIGNFFLLLAT 134
QQI+IG+A+GF Q G+IIG+Q L FA+ VDP+S PV+ +LA
Sbjct: 75 VQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIMDMLAL 134

Query: 135 LIFLAVDGHLLMIRMLVASFDTLPISNVGLTLTSYRSLAEWGSYMFGAALTMSLSAIIAL 194
L+FL +GHL +I +LV +F TLPI L ++ +L + GS +F L ++L I L
Sbjct: 135 LLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLALPLITLL 194

Query: 195 LLVNLSFGVMTRAAPQLNIFSIGFPITMIGGLLILWLTLTPVMAHFDEVWASAQLLLCDI 254
L +NL+ G++ R APQL+IF IGFP+T+ G+ ++ + + + +++ LL DI
Sbjct: 195 LTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADI 254

Query: 255 LGL 257
+
Sbjct: 255 ISE 257


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3217TYPE3IMQPROT471e-10 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 46.7 bits (111), Expect = 1e-10
Identities = 21/73 (28%), Positives = 39/73 (53%)

Query: 4 EALIDIFREALAVIVMMVSAIVLPGLGIGLIVAVFQAATSINEQTLSFLPRLIVTLLALM 63
+ L+ +AL +++++ + IGL+V +FQ T + EQTL F +L+ L L
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 LMGHWLVQTLMDF 76
L+ W + L+ +
Sbjct: 62 LLSGWYGEVLLSY 74


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3218FLGBIOSNFLIP2762e-96 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 276 bits (708), Expect = 2e-96
Identities = 120/240 (50%), Positives = 175/240 (72%)

Query: 8 FIGLSTLLFVTSAGAADGVLPAVTVKTAADGSTEYSVTMQILLLMTSLSFIPAMVIMLTS 67
+ ++ +L A LP +T + G +S+ +Q L+ +TSL+FIPA+++M+TS
Sbjct: 4 LLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMMTS 63

Query: 68 FTRIIVVLSILRQAIGLQQTPSNQVLIGMSLFMTFFIMAPVFDKIYDQGVKPYIDEQLTL 127
FTRII+V +LR A+G P NQVL+G++LF+TFFIM+PV DKIY +P+ +E++++
Sbjct: 64 FTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKISM 123

Query: 128 QQAFDKGKEPLRAFMLGQVRTTDLKTFIDISGYQNINSPEEAPMSVLVPAFITSELKTAF 187
Q+A +KG +PLR FML Q R DL F ++ + PE PM +L+PA++TSELKTAF
Sbjct: 124 QEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKTAF 183

Query: 188 QIGFMLFVPFLVLDLVVASILMAMGMMMLSPMIVSLPFKIMLFVLVDGWGLVMGTLANSF 247
QIGF +F+PFL++DLV+AS+LMA+GMMM+ P ++LPFK+MLFVLVDGW L++G+LA SF
Sbjct: 184 QIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3220FLGMOTORFLIN1124e-35 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 112 bits (281), Expect = 4e-35
Identities = 54/122 (44%), Positives = 81/122 (66%)

Query: 2 STDDDWAAAMAEQALEEANAVGLDELVDDSQPISKAEAAKLDTILDIPVTISMEVGRSFI 61
+ DD WA A+ EQ + +D I+DIPV +++E+GR+ +
Sbjct: 14 ALDDLWADALNEQKATTTKSAADAVFQQLGGGDVSGAMQDIDLIMDIPVKLTVELGRTRM 73

Query: 62 SIRNLLQLNQGSVVELDRVAGEPLDVMVNGTLIAHGEVVVVNDKFGIRLTDVISQTERIK 121
+I+ LL+L QGSVV LD +AGEPLD+++NG LIA GEVVVV DK+G+R+TD+I+ +ER++
Sbjct: 74 TIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSERMR 133

Query: 122 KL 123
+L
Sbjct: 134 RL 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3221FLGMOTORFLIM2451e-81 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 245 bits (628), Expect = 1e-81
Identities = 88/327 (26%), Positives = 164/327 (50%), Gaps = 12/327 (3%)

Query: 1 MSDLLSQDEIDALLHGVDDVEEDNELDAVGQEARS----YDFSSQDRIVRGRMPTLEIVN 56
M+++LSQDEID LL + + E DA YDF D+ + +M TL +++
Sbjct: 1 MTEVLSQDEIDQLLTAISSGDASIE-DARPISDTRKITLYDFRRPDKFSKEQMRTLSLMH 59

Query: 57 ERFARHLRISMFNMMRRAAEVSINGVQMLKFGEYVHTLFVPTSLNMVRFHPLKGTALITM 116
E FAR S+ +R V + V L + E++ ++ P++L ++ PLKG A++ +
Sbjct: 60 ETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEV 119

Query: 117 EARLVFILVDNFFGGDGRFHAKIEGREFTPTERRIVQLLLKIIFEDYKDAWAPVMDVEFD 176
+ + F ++D FGG G+ R+ T E +++ ++ I + +++W V+D+
Sbjct: 120 DPSITFSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPR 177

Query: 177 YLDSEVNPAMANIVSPTEVVVINSFHIEVDGGGGDFHITMPYSMIEPIRELLDAG--VQS 234
E NP A IV P+E+VV+ + +V G + +PY IEPI L + S
Sbjct: 178 LGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSS 237

Query: 235 DKQDTDMRWSQALHDEIMDVKVGFDATVVEHELTLKDVMNFKAGDIIPIE---LPEYIMM 291
++ + ++ L D++ V + A V L+++D++ + GDII + + + ++
Sbjct: 238 VRRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVL 297

Query: 292 RIEDLPTYRCKMGRSRDNLALKIYEKI 318
I + + C+ G +A +I E+I
Sbjct: 298 SIGNRKKFLCQPGVVGKKIAAQILERI 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3223FLGHOOKFLIK532e-09 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 52.5 bits (125), Expect = 2e-09
Identities = 36/132 (27%), Positives = 62/132 (46%), Gaps = 5/132 (3%)

Query: 401 MKQQLVTMVSQGIQQAEIRLDPPELGHMLVKIQVHGDQTQVQFHVAQSQTRDLVEQAIPR 460
+ Q + QG Q AE+RL P +LG + + ++V +Q Q+Q R +E A+P
Sbjct: 244 LSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPV 303

Query: 461 LRELLQEQGMQLADSHVSQGDQGQRREGGFGEAGGSSGGNVDDFSAEELD-----LGLNQ 515
LR L E G+QL S++S +++ + N + + E+ D + L
Sbjct: 304 LRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVSLQG 363

Query: 516 ATSLNSAIDYYA 527
+ NS +D +A
Sbjct: 364 RVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3224FLGFLIJ443e-08 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 43.7 bits (102), Expect = 3e-08
Identities = 37/145 (25%), Positives = 73/145 (50%)

Query: 1 MANTDPLLLVLKLALDAEEQAALLLKSAQLECQKRQNQLDALNNYRLDYMKQMQSQQGQA 60
MA L + LA E AA LL + CQ+ + QL L +Y+ +Y + S
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 ISASHYHQFHRFIRQIDEAIAQQQRVVADGEKQKNYRQHYWLEKQKKRKAVELLLDNKEK 120
I+++ + + +FI+ +++AI Q ++ + ++ + + W EK+++ +A + L + +
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 KRQAIELKKEQKMTDEFASQQFYRR 145
E + +QK DEFA + R+
Sbjct: 121 AALLAENRLDQKKMDEFAQRAAMRK 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3226FLGFLIH902e-23 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 89.9 bits (222), Expect = 2e-23
Identities = 61/202 (30%), Positives = 106/202 (52%), Gaps = 6/202 (2%)

Query: 54 APKAVAVETVAPPTMAEIEDIRAQAEEEGFNEGKTQGHIEGLEQGRLEGLEQGHKEGFTQ 113
AP + P IE+ E++ + + Q H +G + G EG +QGHK+G+ +
Sbjct: 16 APPQAEFVPIVEPEETIIEEAEPSLEQQ-LAQLQMQAHEQGYQAGIAEGRQQGHKQGYQE 74

Query: 114 GHEQGLETGLAEAKA----LLSRFEGLLCQFEKPLQLLDGDIEHTLMSLTMALAKSVIGH 169
G QGLE GLAEAK+ + +R + L+ +F+ L LD I LM + + A+ VIG
Sbjct: 75 GLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQ 134

Query: 170 ELKTHPEQILSALRLGVESLPIKEQTVNLRMHPDDVTLVETLYSSTQLTRNQWQLEADPS 229
++ ++ ++ P+ LR+HPDD+ V+ + +T L+ + W+L DP+
Sbjct: 135 TPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT-LSLHGWRLRGDPT 193

Query: 230 LTAGDCIISSQRSLVDLTLSSR 251
L G C +S+ +D ++++R
Sbjct: 194 LHPGGCKVSADEGDLDASVATR 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3227FLGMOTORFLIG2921e-99 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 292 bits (748), Expect = 1e-99
Identities = 110/348 (31%), Positives = 194/348 (55%), Gaps = 5/348 (1%)

Query: 1 MAENKSKDAAETPSFNVKDLSGIEKTAILLLSLSEADAASILKHLEPKQVQKVGMAMAAM 60
M E K K+ + V L+G +K AILL+S+ ++ + K+L ++++ + +A +
Sbjct: 1 MEEKKEKEILD-----VSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKL 55

Query: 61 EDFGQEKVIGVHKLFLDDIQKYSSIGFNSEEFVRKALTAALGEDKAGNLIEQIIMGSGAK 120
E E V F + + I ++ R+ L +LG KA ++I + ++
Sbjct: 56 ETITSELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSR 115

Query: 121 GLDSLKWMDARQVATIIQNEHPQIQTIVLSYLEPDQAAEIFGQFPENTRLDLMMRIANLE 180
+ ++ D + IQ EHPQ ++LSYL+P +A+ I P + ++ RIA ++
Sbjct: 116 PFEFVRRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMD 175

Query: 181 EVQPAALQELNDIMEKQFAGQGGAQAAKMGGLKAAANIMNYLDTGVESQLMETMRETDEE 240
P ++E+ ++EK+ A GG+ I+N D E ++E++ E D E
Sbjct: 176 RTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPE 235

Query: 241 MAQQIQDLMFVFENLIDVDDRGIQTLLREVQQDVLMKALKGADDQLKDKILGNMSKRAAE 300
+A++I+ MFVFE+++ +DDR IQ +LRE+ L KALK D +++KI NMSKRAA
Sbjct: 236 LAEEIKKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAAS 295

Query: 301 LLRDDLEAMGPIRISEVEIAQKEILSIARRLSDSGEIMLGGGGGDEFL 348
+L++D+E +GP R +VE +Q++I+S+ R+L + GEI++ GG ++ L
Sbjct: 296 MLKEDMEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVL 343


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3228FLGMRINGFLIF3012e-97 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 301 bits (772), Expect = 2e-97
Identities = 160/570 (28%), Positives = 265/570 (46%), Gaps = 56/570 (9%)

Query: 27 LGNLGGVDMMRQITMILALAICLALAVFVMIWAQEPEYRPL-GKMETQEMVQVLDVLDKN 85
L L + +I +I+A + +A+ V +++WA+ P+YR L + Q+ ++ L +
Sbjct: 13 LEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQM 72

Query: 86 KIKYQIDVD--VVKVPEDKYQEVKMMLSRAGIDSAATSKQDFLTQDSGFGVSQRMEQARL 143
I Y+ ++VP DK E+++ L++ G+ + L Q FG+SQ EQ
Sbjct: 73 NIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQ-EKFGISQFSEQVNY 131

Query: 144 KHSQEENLARAIEQLQSVSRAKVILALPKENVFARNTAQPSATVVINTRRG-GLGQGEVD 202
+ + E LAR IE L V A+V LA+PK ++F R PSA+V + G L +G++
Sbjct: 132 QRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQIS 191

Query: 203 AIVDIVASAVQGLEPSRVTVTDSNGRLLNSGSQDGVSARARRELELVQQKETEYRTKIDS 262
A+V +V+SAV GL P VT+ D +G LL + G +L+ E+ + +I++
Sbjct: 192 AVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDL-NDAQLKFANDVESRIQRRIEA 250

Query: 263 ILSPILGHDNFTSQVDVSMDFTAVEQTAKRFNPDLPSLRSEMTVENNST-----GGSTGG 317
ILSPI+G+ N +QV +DF EQT + ++P+ + ++ + + G GG
Sbjct: 251 ILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGG 310

Query: 318 IPGALSNQPP---------------MESNIPQEAATAATESVAAGNSHREATRNFELDTT 362
+PGALSNQP N PQ + + + S ++ R T N+E+D T
Sbjct: 311 VPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRT 370

Query: 363 ISHTRQQIGVVRRVSVSVAVDFKPGAAGENGQVARVARTEQELTNIRRLLEGAVGFSAQR 422
I HT+ +G + R+SV+V V++K A G+ + T ++ I L A+GFS +R
Sbjct: 371 IRHTKMNVGDIERLSVAVVVNYKTLADGKP-----LPLTADQMKQIEDLTREAMGFSDKR 425

Query: 423 GDVLEVVTVPFMDQLVEDVPAPELWEQPWFWRAVKLGVGALVILV----LILAVVRPMLK 478
GD L VV PF + W+Q F + L++LV L VRP L
Sbjct: 426 GDTLNVVNSPF-SAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVRPQLT 484

Query: 479 RLIYPDRVNMPEDGRLGNELAEIEDQYAADTLGMLNTKEAEYSYADDGSIL---IPNLHK 535
R + E E+ E + D +
Sbjct: 485 RRVE----EAKAAQEQAQVRQETEE-------------AVEVRLSKDEQLQQRRANQRLG 527

Query: 536 DDDMIKAIRALVANEPELSTQVVKNWLQDN 565
+ M + IR + N+P + V++ W+ ++
Sbjct: 528 AEVMSQRIREMSDNDPRVVALVIRQWMSND 557


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3229FLGHOOKFLIE595e-15 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 59.3 bits (143), Expect = 5e-15
Identities = 32/101 (31%), Positives = 55/101 (54%)

Query: 12 MQSLKGEVTPSFGISPNNIVQQVNNTSGADFGQLLSQAIGNVSGLQSTSSNLATRLDMGD 71
+Q ++G ++ + + Q+ F L A+ +S Q+ + A + +G+
Sbjct: 3 IQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGE 62

Query: 72 TTVSLSDSVIAREKASVAFEATIQVRNKLVEAYKEIMSMPV 112
V+L+D + +KASV+ + IQVRNKLV AY+E+MSM V
Sbjct: 63 PGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3230HTHFIS465e-164 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 465 bits (1198), Expect = e-164
Identities = 171/484 (35%), Positives = 249/484 (51%), Gaps = 43/484 (8%)

Query: 1 MSEAKLLLVEDDASLREALLDTLMLAQYDCIDVASGEEAILALKQHQFDLVISDVQMPGI 60
M+ A +L+ +DDA++R L L A YD ++ + DLV++DV MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 GGLGLLNYLQQHHPKLPVLLMTAYATIGSAVSAIKLGAVDYLAKPFAPEVLLNQVSRYLP 120
LL +++ P LPVL+M+A T +A+ A + GA DYL KPF L+ + R L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 LKQNRDQPVVAD-----------EKSLSLLSLAQRVAASDASVMILGPSGSGKEVLARYI 169
+ R + D + + R+ +D ++MI G SG+GKE++AR +
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180

Query: 170 HQHSSRAEEAFVAINCAAIPENMLEATLFGYEKGAFTGAYQACPGKFEQAQGGTLLLDEI 229
H + R FVAIN AAIP +++E+ LFG+EKGAFTGA G+FEQA+GGTL LDEI
Sbjct: 181 HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI 240

Query: 230 SEMDLGLQAKLLRVLQEREVERLGGRKTIKLDVRVLATSNRDLKAVVAAGQFREDLYYRI 289
+M + Q +LLRVLQ+ E +GGR I+ DVR++A +N+DLK + G FREDLYYR+
Sbjct: 241 GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300

Query: 290 NVFPLTWPALNQRPADILPLARHLLTKHAKALNVVDLPEFDDAACRRLLSHRWPGNVREL 349
NV PL P L R DI L RH + + K V FD A + +H WPGNVREL
Sbjct: 301 NVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDV--KRFDQEALELMKAHPWPGNVREL 358

Query: 350 DNVVQRALILRAGALITANDIIIDAQDVPLTSDD-------------------------- 383
+N+V+R L +IT I + + S
Sbjct: 359 ENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFAS 418

Query: 384 -AEYMSEPEGLGEELKAQEHVIILETLVQCQGSRKLVAEKLGISARTLRYKMARMRDMGI 442
+ + L E+ +IL L +G++ A+ LG++ TLR K +R++G+
Sbjct: 419 FGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKK---IRELGV 475

Query: 443 QLPS 446
+
Sbjct: 476 SVYR 479


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3232HTHFIS441e-154 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 441 bits (1135), Expect = e-154
Identities = 168/479 (35%), Positives = 260/479 (54%), Gaps = 17/479 (3%)

Query: 7 RILLVGTPSERLSRLCCIFEFLGEQIDII-TIEKLSTCLQETRFRALVISI---DNMSAD 62
IL+ + + L G + I L + +V + D + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 ALKLVASQYPWQPILLFGHVGELQV------SNVLGHIEEPLNYPQLTELLHFCQVYGQV 116
L + P P+L+ ++ +P + +L ++ +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 117 KRPQVPTSANQTKLFRSLVGRSDGIANVRHLISQVATSDATVLVLGQSGTGKEVVARNIH 176
+ ++ + LVGRS + + +++++ +D T+++ G+SGTGKE+VAR +H
Sbjct: 125 RPSKLEDDSQD---GMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALH 181

Query: 177 YLSERRDGPFIPVNCGAIPPELLESELFGHEKGSFTGAISSRKGRFELAEGGTLFLDEIG 236
+RR+GPF+ +N AIP +L+ESELFGHEKG+FTGA + GRFE AEGGTLFLDEIG
Sbjct: 182 DYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG 241

Query: 237 DMPLQMQVKLLRVLQERVFERVGGTKTINADVRVVAATHRDLETMISSNEFREDLYYRLN 296
DMP+ Q +LLRVLQ+ + VGG I +DVR+VAAT++DL+ I+ FREDLYYRLN
Sbjct: 242 DMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLN 301

Query: 297 VFPIEMPALSERKDDVPLLLQELVSRVYNEGRGKVRFTQRAIESLKEHAWSGNVRELSNL 356
V P+ +P L +R +D+P L++ V + EG RF Q A+E +K H W GNVREL NL
Sbjct: 302 VVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELENL 361

Query: 357 VERLTILYPGGLVDVNDLPIKYRHIDVPEYSVELSEEQQERDALASIFTSEEPVEIPETR 416
V RLT LYP ++ + + R ++P+ +E + + +++ EE +
Sbjct: 362 VRRLTALYPQDVITREIIENELRS-EIPDSPIEKAAARSGSLSISQAV--EENMRQYFAS 418

Query: 417 FPSELPPEGVNLKDLLAELEIDMIRQALEQQDNVVARAAEMLGIRRTTLVEKMRKYGMT 475
F LPP G+ +LAE+E +I AL +AA++LG+ R TL +K+R+ G++
Sbjct: 419 FGDALPPSGL-YDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3235INTIMIN340.002 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 33.5 bits (76), Expect = 0.002
Identities = 50/260 (19%), Positives = 78/260 (30%), Gaps = 27/260 (10%)

Query: 3 ISATGMGSGLDISNIVKVLVDAEKAPKEAMFNKTEDSIKAKVSAMGTLKSALSTFQDAVK 62
++A + SN V + + + D K SA A+ T+ VK
Sbjct: 527 VTARAYDRNGNSSNNVLLTITVLSNGQVVDQVGVTDFTADKTSAKADGTEAI-TYTATVK 585

Query: 63 KLQTGDALNQRKIS-VSTDAYLSAKAEKTAQAGSYGIKVEQVATNHKIAGTNVADATQPV 121
K A + VS A LSA + T +G + + + K V +
Sbjct: 586 KNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVTL----KSDKPGQVVV---SAKT 638

Query: 122 GEGSLAFGVNGKSFSVDIAATDSLAEIAKKVNEASDNVGVTAT--------------VIT 167
E + A N F A+ + + K A+ +T T V
Sbjct: 639 AEMTSALNANAVIFVDQTKASITEIKADKTTAVANGQDAITYTVKVMKGDKPVSNQEVTF 698

Query: 168 SDAGSRLVFSANKTGEDNQISLTAT-NTSGSG-VTDMFGSGNTSTLQDAKN--AVLYIDG 223
+ +L S KT + +T T T G V+ L ID
Sbjct: 699 TTTLGKLSNSTEKTDTNGYAKVTLTSTTPGKSLVSARVSDVAVDVKAPEVEFFTTLTIDD 758

Query: 224 QKVTSQSNEVKNAITGVTLN 243
+ VK + V L
Sbjct: 759 GNIEIVGTGVKGKLPTVWLQ 778


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3237FLAGELLIN1337e-38 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 133 bits (335), Expect = 7e-38
Identities = 93/271 (34%), Positives = 129/271 (47%), Gaps = 10/271 (3%)

Query: 2 AITVNTNVTSLKAQKNLNTSASDLATSMERLSSGLRINSAKDDAAGLAISNRLNSQVRGL 61
A +NTN SL Q NLN S S L++++ERLSSGLRINSAKDDAAG AI+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 EVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTVQSENGANSSADLSALKAEMDQLAN 121
RNAND ISIAQ EGA+ E N LQR+R+L+VQ+ NG NS +DL +++ E+ Q
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EIDEIGKTTAFGTTKLLAGGFSAGKNFQVGAQDGEDIKVTVKASNKSSLSVGSL------ 175
EID + T F K+L+ QVGA DGE I + ++ + SL +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQ--MKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPK 178

Query: 176 --GNTTSAARASSLKKIDAAIKTIDAQRADLGAIQNRLAHNISNSANTQANVADAKSRIV 233
+ ++ D + R D+ + + A
Sbjct: 179 EATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTT 238

Query: 234 DVDFAKETSQMTKNQVLQQTGSAMLAQANQL 264
D + K + A A +
Sbjct: 239 DDAENNTAVDLFKTTKSTAGTAEAKAIAGAI 269



Score = 85.9 bits (212), Expect = 4e-21
Identities = 61/213 (28%), Positives = 98/213 (46%), Gaps = 4/213 (1%)

Query: 60 GLEVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTVQSENGANSSADLSALKAEMDQL 119
+ + +++A I GA LQ +++ NG + D + ++
Sbjct: 298 KVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSD 357

Query: 120 ANEIDEIGKTTAFGTTKLLAGGFSAGKNFQVGAQDGEDIKVTVKASNKSSLSVGSLGNTT 179
+ + + +AG + + K ++ S +
Sbjct: 358 LEANNAVKGESKITVNGAEYTANAAGDKVTLAGK----TMFIDKTASGVSTLINEDAAAA 413

Query: 180 SAARASSLKKIDAAIKTIDAQRADLGAIQNRLAHNISNSANTQANVADAKSRIVDVDFAK 239
+ A+ L ID+A+ +DA R+ LGAIQNR I+N NT N+ A+SRI D D+A
Sbjct: 414 KKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYAT 473

Query: 240 ETSQMTKNQVLQQTGSAMLAQANQLPQVALSLL 272
E S M+K Q+LQQ G+++LAQANQ+PQ LSLL
Sbjct: 474 EVSNMSKAQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3238FLAGELLIN1335e-38 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 133 bits (336), Expect = 5e-38
Identities = 95/271 (35%), Positives = 128/271 (47%), Gaps = 10/271 (3%)

Query: 2 AITVNTNVTSMKAQKNLNTSNSGLSTSMERLSSGLRINSAKDDAAGLAISNRLNSQVRGL 61
A +NTN S+ Q NLN S S LS+++ERLSSGLRINSAKDDAAG AI+NR S ++GL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 EVGMRNANDAISIAQIAEGAMQEQTNMLQRMRDLTIQSENGANSTADLVSIKAEMDQLAT 121
RNAND ISIAQ EGA+ E N LQR+R+L++Q+ NG NS +DL SI+ E+ Q
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EIDSIGNSTAFGNTKLLTGTFSAGKVFQVGHQEGEDIKVTVKASNKTSLSVGALNNATSA 181
EID + N T F K+L+ QVG +GE I + ++ + SL + N
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQ--MKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPK 178

Query: 182 NRASSLAK--------IDAAIKTIDSQRADLGAVQNRLAHNISNSANTQANVADAKSRIV 233
K D + R D+ + + A
Sbjct: 179 EATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTT 238

Query: 234 DVDFAKETSAMTKYQVLQQTGSAMLAQANQL 264
D + K + A A +
Sbjct: 239 DDAENNTAVDLFKTTKSTAGTAEAKAIAGAI 269



Score = 87.0 bits (215), Expect = 1e-21
Identities = 52/146 (35%), Positives = 76/146 (52%)

Query: 127 GNSTAFGNTKLLTGTFSAGKVFQVGHQEGEDIKVTVKASNKTSLSVGALNNATSANRASS 186
N+ + + G K ++ S + A + A+
Sbjct: 361 NNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANP 420

Query: 187 LAKIDAAIKTIDSQRADLGAVQNRLAHNISNSANTQANVADAKSRIVDVDFAKETSAMTK 246
LA ID+A+ +D+ R+ LGA+QNR I+N NT N+ A+SRI D D+A E S M+K
Sbjct: 421 LASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSK 480

Query: 247 YQVLQQTGSAMLAQANQLPQVALSLL 272
Q+LQQ G+++LAQANQ+PQ LSLL
Sbjct: 481 AQILQQAGTSVLAQANQVPQNVLSLL 506


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3239FLAGELLIN552e-10 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 55.4 bits (133), Expect = 2e-10
Identities = 65/362 (17%), Positives = 118/362 (32%), Gaps = 16/362 (4%)

Query: 20 QTATSKVLEQLSSGKKVNTSGDDPVAALGIDNLNQRNALVDQFMKNIDYANNRLAVTESK 79
Q++ S +E+LSSG ++N++ DD + + Q +N + + TE
Sbjct: 21 QSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNANDGISIAQTTEGA 80

Query: 80 LGSAEDLTGSIREQLMRAVNGTLSDSERQMIADEMKDSLKELLSLANSKDESGNYLFSG- 138
L + +RE ++A NGT SDS+ + I DE++ L+E+ ++N +G + S
Sbjct: 81 LNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKVLSQD 140

Query: 139 FSTDKQPFAFDNST---PPKIVYSGDSGVRDSLVQSGVAIGTNIPGDQAFMKAPNGLGDY 195
Q A D T + + G+ V GD D
Sbjct: 141 NQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATV---GDLKSSFKNVTGYDT 197

Query: 196 SVNYLASQQGDFKVQTAKIADQATYVADTYTFNFSDNGAGGVNLQVLDSANNPVANVTNF 255
+ D A V D N ++ V F
Sbjct: 198 YAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTT-------DDAENNTAVDLF 250

Query: 256 DATTPVSFNGIEVKIDGKPSAGDSFSMEPQSEVSIFDTISNAIALIEDPNVANSPQGKAQ 315
T + I G G V+ TI + V+ + G+
Sbjct: 251 KTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTF--TIDTKTGNDGNGKVSTTINGEKV 308

Query: 316 LAQILNNIDSGVNQMSSARSVAGNNLKTVERYKDTHTEEKVVNTSALSLLEDLDYASAIT 375
+ + N ++ + N +V + T ++ ++ LS LE + +
Sbjct: 309 TLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGES 368

Query: 376 EF 377
+
Sbjct: 369 KI 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3240FLGHOOKAP12145e-64 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 214 bits (547), Expect = 5e-64
Identities = 123/455 (27%), Positives = 194/455 (42%), Gaps = 19/455 (4%)

Query: 4 DLLNIARTGVLASQSQLGVTSNNIANANTVGYHRQVATQTTLDSQRLGSSFYGTGTYVSD 63
L+N A +G+ A+Q+ L SNNI++ N GY RQ +S + G G YVS
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSG 61

Query: 64 VKRIYNDYAARELRIGQTTLSAAEASYGKLSELDQIFSQIGKVVPQSLTDLFAGLNSLAD 123
V+R Y+ + +LR QT S A Y ++S++D + S + + D F L +L
Sbjct: 62 VQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVS 121

Query: 124 LPADLGIRSSTLNDAKQLANSLNQMQSTLNGQLNQTNDQITGMTKRINEISTELANLNLE 183
D R + + ++ L N L Q Q N I +IN + ++A+LN +
Sbjct: 122 NAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLNDQ 181

Query: 184 LMKSPNQDPI-----LLDKQDALVQELSQYAQVNVIPLENGAKSIMLGGSIMLVSGEVA- 237
+ + LLD++D LV EL+Q V V + G +I + LV G A
Sbjct: 182 ISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTAR 241

Query: 238 -MSMSTATGDPFPNELQLMSSIGSQSVKADPSKLGGQLGALFEYRDQTLVPASLELDQLA 296
++ ++ DP + + + G LG + +R Q L L QLA
Sbjct: 242 QLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLA 301

Query: 297 LGVADSFNKLQAQGFDLNGQVGANMFKDINDPLMSLGRVGGFTDNTGNATLGVNIDDTSA 356
L A++FN GFD NG G + F + V T N G+ +G + D SA
Sbjct: 302 LAFAEAFNTQHKAGFDANGDAGEDFFA------IGKPAVLQNTKNKGDVAIGATVTDASA 355

Query: 357 LTGGSYELSF--TAPSTYELRDTQTGAITPLTLNGTKLEGGAGFSIDIKAGAMASGDRFS 414
+ Y++SF L T +TP +G G A D F+
Sbjct: 356 VLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFT----GTPAVNDSFT 411

Query: 415 IRPTAGAANGIEVVMTDPKGIAAAAPKITADTANS 449
++P + A ++V++TD IA A+ + D+ N
Sbjct: 412 LKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNR 446



Score = 83.1 bits (205), Expect = 8e-19
Identities = 38/103 (36%), Positives = 54/103 (52%)

Query: 535 AEGDNTNAVAMAKLSETKVMNGGKSTLADVFELTKQDIGSKTKAAEVRVGSAEAIYKQAY 594
+ DN N A+ L GG + D + DIG+KT + + + Q
Sbjct: 441 GDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLS 500

Query: 595 ARVESESGVNLDEEAANLMRFQQAYQASARIMSTAQQIFDSLL 637
+ +S SGVNLDEE NL RFQQ Y A+A+++ TA IFD+L+
Sbjct: 501 NQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALI 543


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3241FLGFLGJ1545e-46 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 154 bits (389), Expect = 5e-46
Identities = 66/160 (41%), Positives = 96/160 (60%), Gaps = 2/160 (1%)

Query: 214 RETQKTLKFGSREEFLATLYPHAEKAAKALGTKPEVLLAQSALETGWGQKIVRGHNGAPS 273
R +L S+ FLA L A+ A++ G ++LAQ+ALE+GWGQ+ +R NG PS
Sbjct: 139 RNYDDSLPGDSKA-FLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPS 197

Query: 274 HNLFNIKADRRWQGDKANVSTLEFEQGIAVRQKADFRVYNDFDHSFNDFVSFIADGERYQ 333
+NLF +KA W+G ++T E+E G A + KA FRVY+ + + +D+V + RY
Sbjct: 198 YNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYA 257

Query: 334 DAKKVAASPTQFIRALQEAGYATDPKYAEKVIKVMQSISE 373
A AAS Q +ALQ+AGYATDP YA K+ ++Q +
Sbjct: 258 -AVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKS 296



Score = 88.2 bits (218), Expect = 8e-22
Identities = 39/93 (41%), Positives = 61/93 (65%), Gaps = 3/93 (3%)

Query: 12 DLGGLDNLRAQAQKDEKGALKKVAQQFEGVFVQMLMKSMRDANAVFESDSPLNSQYTKFY 71
D L+ L+A+A +D ++ VA+Q EG+FVQM++KSMRDA D +S++T+ Y
Sbjct: 14 DAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALP---KDGLFSSEHTRLY 70

Query: 72 EQMRDQQLSVDLSDKGVLGLADMMVQQLSPESS 104
M DQQ++ ++ LGLA+MMV+Q++PE
Sbjct: 71 TSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQP 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3242FLGPRINGFLGI380e-133 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 380 bits (976), Expect = e-133
Identities = 157/362 (43%), Positives = 218/362 (60%), Gaps = 12/362 (3%)

Query: 8 AVFMLAFSMPSQAERIKDIANVQGVRNNQLIGYGLVVGLPGTGEK---TNYTEQTFTTML 64
F+ + RIKDIA++Q R+NQLIGYGLVVGL GTG+ + +TEQ+ ML
Sbjct: 16 LPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAML 75

Query: 65 KNFGINLPENFRPKIKNVAVVAVHADMPAFIKPGQELDVTVSSLGEAKSLRGGTLLQTFL 124
+N GI + KN+A V V A++P F PG +DVTVSSLG+A SLRGG L+ T L
Sbjct: 76 QNLGITTQGG-QSNAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSL 134

Query: 125 KGVDGNVYAIAQGSLVVSGFSADGLDGSKVIQNTPTVGRIPNGAIVERSVATPFSTGDYL 184
G DG +YA+AQG+L+V+GFSA G D + + Q T R+PNGAI+ER + + F L
Sbjct: 135 SGADGQIYAVAQGALIVNGFSAQG-DAATLTQGVTTSARVPNGAIIERELPSKFKDSVNL 193

Query: 185 TFNLRRSDFSTAQRMADAINDL----LGPDMARPLDATSIQVSAPRDVSQRVSFLATLEN 240
LR DFSTA R+AD +N G +A P D+ I V PR V+ +A +EN
Sbjct: 194 VLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPR-VADLTRLMAEIEN 252

Query: 241 IEVEPAEESAKVIVNSRTGTIVVGQNVKLLPAAVTHGGLTVTIAEATQVSQPNALANGQT 300
+ VE + AKV++N RTGTIV+G +V++ AV++G LTV + E+ QV QP + GQT
Sbjct: 253 LTVET-DTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQT 311

Query: 301 TVTSNSTINATESDRRMFMFNPGTTLDELVRAVNLVGAAPSDVLAILEALKVAGALHGEL 360
V + I A + ++ + G L LV +N +G ++AIL+ +K AGAL EL
Sbjct: 312 AVQPQTDIMAMQEGSKVAIVE-GPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370

Query: 361 II 362
++
Sbjct: 371 VL 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3243FLGLRINGFLGH1502e-47 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 150 bits (379), Expect = 2e-47
Identities = 76/215 (35%), Positives = 107/215 (49%), Gaps = 9/215 (4%)

Query: 11 LLLTACSSTPKKPIADDPFYAPVYPEAPPTKIAATGSIYQDSQAA-----SLYSDIRAHK 65
L LT C+ P P+ A P P A GSI+Q +Q L+ D R
Sbjct: 17 LSLTGCAWIPSTPLVQGATSAQPVPGPTP---VANGSIFQSAQPINYGYQPLFEDRRPRN 73

Query: 66 VGDIITIVLKEATQAKKSAGNQIKKGSDMTLDPVYAGGSNLSL-GGIPLDLRYKDSMNTK 124
+GD +TIVL+E A KS+ + L G D+
Sbjct: 74 IGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGGNTFN 133

Query: 125 RESDADQSNSLDGSISANIMQVLNNGNLVVRGEKWISINNGDEFIRVTGIVRSQDIKPDN 184
+ A+ SN+ G+++ + QVL NGNL V GEK I+IN G EFIR +G+V + I N
Sbjct: 134 GKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTISGSN 193

Query: 185 TIDSTRMANARIQYSGTGTFAEAQKVGWLSQFFMS 219
T+ ST++A+ARI+Y G G EAQ +GWL +FF++
Sbjct: 194 TVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLN 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3244FLGHOOKAP1452e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.6 bits (105), Expect = 2e-07
Identities = 19/119 (15%), Positives = 41/119 (34%), Gaps = 4/119 (3%)

Query: 145 EDATSITVSAEGEVSVKTPGAAENQVVGQLSMTDFINPSGLDPMGQNLYTETG---ASGT 201
D I +++E + + + Q + + +L ++ G A+
Sbjct: 427 TDEAKIAMASEEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLK 486

Query: 202 PIQGTASLDGMGAIRQGALETSNVNVTEELVNLIESQRIYEMNSKVISAVDQMLAYVNQ 260
T + + S VN+ EE NL Q+ Y N++V+ + + +
Sbjct: 487 TSSATQGNV-VTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544



Score = 36.5 bits (84), Expect = 9e-05
Identities = 9/36 (25%), Positives = 21/36 (58%)

Query: 5 LWISKTGLDAQQTDISVISNNVANASTVGYKKSRAV 40
+ + +GL+A Q ++ SNN+++ + GY + +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI 39


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3247FLGHOOKAP1401e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 40.3 bits (94), Expect = 1e-05
Identities = 15/35 (42%), Positives = 22/35 (62%)

Query: 2 SFNIALSGISAAQKDLNTTANNIANANTIGFKESR 36
N A+SG++AAQ LNT +NNI++ N G+
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQT 37



Score = 37.2 bits (86), Expect = 1e-04
Identities = 13/49 (26%), Positives = 25/49 (51%)

Query: 405 SLSSSALEQSNIDLTTELVDLISAQRNFQANSRTLEVNNTLQQTVLQIR 453
LS+ S ++L E +L Q+ + AN++ L+ N + ++ IR
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3249FLGHOOKAP1333e-04 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 33.0 bits (75), Expect = 3e-04
Identities = 9/38 (23%), Positives = 18/38 (47%)

Query: 99 NVNVMEEMADMISASRSYQMNVQVAEAAKSMLQQTLGM 136
VN+ EE ++ + Y N QV + A ++ + +
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 28.4 bits (63), Expect = 0.010
Identities = 15/64 (23%), Positives = 27/64 (42%), Gaps = 6/64 (9%)

Query: 8 DVAGSGMSAQSLRLNTTASNIANADSVSSSIDKTYRSRHPIFEAEMAKAQSQQQASQGVA 67
+ A SG++A LNT ++NI++ + Y + I + + GV
Sbjct: 5 NNAMSGLNAAQAALNTASNNISSYN------VAGYTRQTTIMAQANSTLGAGGWVGNGVY 58

Query: 68 VKGI 71
V G+
Sbjct: 59 VSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3252HTHFIS612e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 60.6 bits (147), Expect = 2e-12
Identities = 23/128 (17%), Positives = 53/128 (41%), Gaps = 12/128 (9%)

Query: 180 HIMVIDDSAVARKQIIRSLESLNLQIDTAKDGREALDKLKAIASEMNNVADEIPLIISDI 239
I+V DD A R + ++L + + + A + L+++D+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAA---------GDGDLVVTDV 55

Query: 240 EMPEMDGYTLTAEIRDDPKLKNIKVVLHTSLSGVFNQAMVQKVGANDFIAK-FNPDELAA 298
MP+ + + L I+ ++ V++ ++ + + GA D++ K F+ EL
Sbjct: 56 VMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIG 113

Query: 299 AVNKHLSL 306
+ + L+
Sbjct: 114 IIGRALAE 121


73SO_3271SO_3287N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3271014-3.324298bifunctional UDP GlcNAc C6 dehydratase/C5
SO_3272-114-3.397008ISSod6 transposase TnpA_ISSod6
SO_3273-213-2.534080motility accessory factor Maf family
SO_3274-2130.771346protein of unknown function DUF2947
SO_3275-2130.843987putative periplasmic protein
SO_3276-4140.478183outer membrane protein FadL family
SO_3277-3160.308634transcriptional repressor of RND efflux
SO_3278-2150.315063RND superfamily efflux pump MFP component
SO_3279-213-0.131568RND superfamily efflux pump permease component
SO_3280-1150.713799protein of unknown function DUF465
SO_3282-1140.731478energy taxis modulating methyl accepting sensory
SO_3284-1161.349697protein YbgT
SO_3285-1161.505525cytochrome d ubiquinol oxidase subunit II CydB
SO_32860171.749915cytochrome d ubiquinol oxidase subunit I CydA
SO_32870142.238817phosphoribosylformylglycinamidine synthase PurL
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3271NUCEPIMERASE803e-19 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 80.2 bits (198), Expect = 3e-19
Identities = 43/245 (17%), Positives = 86/245 (35%), Gaps = 54/245 (22%)

Query: 6 TILITGGTGSFGQKYTKTILERY-----------------KPKRLIIFSRDELKQYEMQQ 48
L+TG G G +K +LE K RL + ++ + ++
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHK--- 58

Query: 49 VFNAPCMRYFIGDVRDGERLKQAFKDVDF--VIHAAALKQVPAAEYNPMECIKTNIHGAE 106
D+ D E + F F V + V + NP +N+ G
Sbjct: 59 -----------IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFL 107

Query: 107 NVIRAAISNNVKKVIALST---------------DKAASPINLYGATKLASDKLFVAANN 151
N++ N ++ ++ S+ D P++LY ATK A++ + ++
Sbjct: 108 NILEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH 167

Query: 152 VVGDGKTRFAAVRYGNVVGSRGS---VVPFFKQLIANGATSLPITHPDMTRFWITLQDGV 208
+ G +R+ V G G + F + + G + + M R + + D
Sbjct: 168 LYG---LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIA 224

Query: 209 DFVLK 213
+ +++
Sbjct: 225 EAIIR 229


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3273SYCDCHAPRONE290.038 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 29.1 bits (65), Expect = 0.038
Identities = 20/91 (21%), Positives = 35/91 (38%), Gaps = 7/91 (7%)

Query: 728 TLVKILAYSDEYMPQ---YAHILKLCGKIQESVNVY--LDYLKKYASDTQTWIKLGLFMI 782
T+ + S + + Q A GK +++ V+ L L Y S ++ LG
Sbjct: 24 TIAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRF--FLGLGACRQ 81

Query: 783 ELGQAEGAHTAFSNALNADPSNQVAQHYVAE 813
+GQ + A ++S D + AE
Sbjct: 82 AMGQYDLAIHSYSYGAIMDIKEPRFPFHAAE 112


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3277HTHTETR706e-17 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 69.7 bits (170), Expect = 6e-17
Identities = 30/145 (20%), Positives = 54/145 (37%), Gaps = 5/145 (3%)

Query: 11 RSEQKKQQVLVAAIDLFCRQGFPHTSMDEVAKLAGVSKQTVYSHYGSKDELFVAAIE--S 68
+++ +Q +L A+ LF +QG TS+ E+AK AGV++ +Y H+ K +LF E
Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67

Query: 69 KCVGHNLNDDLLSDPSQPEATITEFALQFGEMIVSPEAITVFKACVAQSESHP---EVSR 125
+G + P P + + E + E V+ E + + V +
Sbjct: 68 SNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQ 127

Query: 126 LFFEAGPKHIVGLMADYLVAVEALG 150
+ L
Sbjct: 128 QAQRNLCLESYDRIEQTLKHCIEAK 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3278RTXTOXIND431e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 43.3 bits (102), Expect = 1e-06
Identities = 32/158 (20%), Positives = 53/158 (33%), Gaps = 18/158 (11%)

Query: 101 RLLEAERQ--EIQASLAQTQADVDLATSTL---KRNLELKKSGYVSEQL--LDETQTQLA 153
+LE E + E L ++ ++ S + K +L + +E L L +T +
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 154 SLEAAKKRLLASQHANQLKLDKSHLLAPFDGIISQRQ-HNLGEVVAAGSPAFTLVGSMNT 212
L N+ + S + AP + Q + H G VV +V +T
Sbjct: 313 LLTLELA-------KNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDT 365

Query: 213 -EAYIGVPVAIANQFSTGQNV--KVSVHNQPFTAKIAG 247
E V + GQN KV + G
Sbjct: 366 LEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVG 403



Score = 39.0 bits (91), Expect = 3e-05
Identities = 30/137 (21%), Positives = 52/137 (37%), Gaps = 14/137 (10%)

Query: 33 ATTAQLQNVITAPLRLSPSYQSEQVFTGTIRAGNTTGVGFELSGKLSELNVDSGANVHQG 92
+ Q++ V TA +L+ S G + + + + E+ V G +V +G
Sbjct: 75 SVLGQVEIVATANGKLTHS-------------GRSKEIKPIENSIVKEIIVKEGESVRKG 121

Query: 93 QVLAKLDTRLLEAERQEIQASLAQTQADVDLATSTLKRNLELKKSGYVSEQLLDETQTQL 152
VL KL EA+ + Q+SL Q + + L R++EL K + Q
Sbjct: 122 DVLLKLTALGAEADTLKTQSSLLQARLEQ-TRYQILSRSIELNKLPELKLPDEPYFQNVS 180

Query: 153 ASLEAAKKRLLASQHAN 169
L+ Q +
Sbjct: 181 EEEVLRLTSLIKEQFST 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3279ACRIFLAVINRP381e-118 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 381 bits (980), Expect = e-118
Identities = 213/1052 (20%), Positives = 428/1052 (40%), Gaps = 62/1052 (5%)

Query: 1 MIKAFVENGRLVSLVIALLLVAGFGAMSSLPRTEDPHITNRFASVITPYPGASAERVEAL 60
M F+ ++ +L++AG A+ LP + P I SV YPGA A+ V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTEVLENQLRRLEEIKLIQSTS-RPGISVIQLELKDTVTDTDPVWSR--ARDLLADARNS 117
VT+V+E + ++ + + STS G I L + TDP ++ ++ L A
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQS---GTDPDIAQVQVQNKLQLATPL 117

Query: 118 LPDDIQTPTL-DDQVGYAYTAILSLVWNDSSQPRVDMLNRYAKELQSRLRLLAGTDFVKL 176
LP ++Q + ++ +Y + V ++ + D+ + A ++ L L G V+L
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 177 YGAPEEEILVQLDGYKMSQLQLTPGTIANILRSADSKIAAGEINN------NHFRALVEV 230
+GA + + + LD +++ +LTP + N L+ + +IAAG++ A +
Sbjct: 178 FGA-QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 231 SGELDSQSRIRQVPLKVDSDGQIIRLGDIATISRQPKSPADSIGLVDGEQGVFVAARMLN 290
+ +V L+V+SDG ++RL D+A + + I ++G+ + ++
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARV-ELGGENYNVIARINGKPAAGLGIKLAT 295

Query: 291 NTRVDIWQGQVKQLVDEFNIDLPANIKVEWLLEQNSYTSERLGGLVINLIQGFVIILSVL 350
+K + E P +KV + + + + +V L + +++ V+
Sbjct: 296 GANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVM 355

Query: 351 LLTLG-LRNAVIVAISLPLTALFTLACMKYIGLPIHQMSVTGLVVALGIMVDNAIVIVDA 409
L L +R +I I++P+ L T A + G I+ +++ G+V+A+G++VD+AIV+V+
Sbjct: 356 YLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVEN 415

Query: 410 IAQRRQ-QGMSRLNAVTEALHHLWLPLAGSTITTILAFAPIVLMPGATGEFVGGIAMAVM 468
+ + + A +++ + L G + F P+ G+TG ++ ++
Sbjct: 416 VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIV 475

Query: 469 FALFGSYLISHTLIAGLAGRF-------SLEGKNPI--WYQHGINLPLVSGYFQASLRFA 519
A+ S L++ L L E K W+ + V+ Y S+
Sbjct: 476 SAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFD-HSVNHY-TNSVGKI 533

Query: 520 LNRPRLSAILIGTIPLLGFYASGKMTEQFFPPSDRDMFQIELYLAPHVSLENT---LSQV 576
L ++ I ++ F P D+ +F + L + E T L QV
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQV 593

Query: 577 --YLMDKQLHQIEGITQVDWV-VGGNTPSFYYNLTQRQQGATNYAQAMIK-----TIDFE 628
Y + + +E + V+ G + A +K D
Sbjct: 594 TDYYLKNEKANVESVFTVNGFSFSGQAQN------------AGMAFVSLKPWEERNGDEN 641

Query: 629 RANALIPVLQQRFDK---AFPEAQVLVRKLEQGPPFNAPVELM-IFGPDLETLRTLGDEI 684
A A+I + K F + +E G EL+ G + L +++
Sbjct: 642 SAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQL 701

Query: 685 RHILAATP-DVIHTRATLSAGSPKVLLQVNEDASLISGLSLTNIARQVQMSTTGVIGGSV 743
+ A P ++ R + + L+V+++ + G+SL++I + + + G
Sbjct: 702 LGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDF 761

Query: 744 LEQTESLPIRVRLGENSREQASRLSEIQLMTPSGTAVPLSALSHNEVQVSRGAIPRRNGQ 803
+++ + V+ R + ++ + + +G VP SA + + + R NG
Sbjct: 762 IDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGL 821

Query: 804 RVNTIEAYIVSGVLPAQVLNDVKDKVAAIKLPAGYRIEIGGESAKRNEAIGNLLSNIILV 863
I+ G + +++ A KLPAG + G S + + + + +
Sbjct: 822 PSMEIQGEAAPGTSSGDAMALMEN--LASKLPAGIGYDWTGMSYQERLSGNQAPALVAIS 879

Query: 864 VTLLLATVVLSFNSFRLTAIILLSALQSAGLGLLAVFVFGYPFGFPVIIALLGLMGLAIN 923
++ + + S+ + ++L LLA +F ++ LL +GL+
Sbjct: 880 FVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAK 939

Query: 924 AAIVILAELEELPAAR-MGDKAVIVSTVSSCGRHISSTTITTVGGFIPLII---AGGGFW 979
AI+I+ ++L G + V R I T++ + G +PL I AG G
Sbjct: 940 NAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQ 999

Query: 980 PPFAIAIAGGTLLTTLLSLVWVPTMYLLLMTK 1011
I + GG + TLL++ +VP ++++
Sbjct: 1000 NAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3282IGASERPTASE300.037 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.0 bits (67), Expect = 0.037
Identities = 37/236 (15%), Positives = 80/236 (33%), Gaps = 11/236 (4%)

Query: 393 QTSVQSIEQQASKAQRIAKQNGEEAQALMQQTDQIATAIEEMSTSIRDVANHAQEGANQN 452
+V+ EQ A++ ++ +EA++ ++ Q + S + +E A
Sbjct: 1048 SKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVE 1107

Query: 453 QQVDIAAKEGQQQQTRLVQDLLKLSQQLNNSHQAVDKVSQESEAISKVTEVINSIAEQTN 512
++ + + Q+ V + Q+ + + Q + ++E++ T I QTN
Sbjct: 1108 KEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDP----TVNIKEPQSQTN 1163

Query: 513 LLA--LNAAIEAARAGEQGRGFAVVADEVRTLAKRTQTSILEIGQTIDKLQTQVKTTTSQ 570
A A E + EQ V + + E T ++++
Sbjct: 1164 TTADTEQPAKETSSNVEQ-----PVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNK 1218

Query: 571 MAQSHQLGIASANQGEETGHQLEEINRRIAELAISSRNIASATEQQSSVAQEITHN 626
H+ + S E +A ++S N + + AQ + N
Sbjct: 1219 PKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALN 1274


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3287OMS28PORIN310.031 OMS28 porin signature.
		>OMS28PORIN#OMS28 porin signature.

Length = 257

Score = 30.9 bits (69), Expect = 0.031
Identities = 32/138 (23%), Positives = 61/138 (44%), Gaps = 11/138 (7%)

Query: 138 VLNDFAKADVLFKRTEPAPFKSVNVLAEGRRAL------EVANVEMGLALAEDEIDYLVE 191
+++D AK V+ + K ++AEG + V + +++A E +L+E
Sbjct: 102 LMSDVAKGTVVASQEATIVAKCSGMVAEGANKVVEMSKKAVQETQKAVSVA-GEATFLIE 160

Query: 192 NFVRLNRNPNDIELMMFAQ--ANSEHCRHKIFNADWTIDGKAQ-PKSLFKMIKNTFEVTP 248
+ LN++PN+ EL + + A E + + ++ +D Q + + M+
Sbjct: 161 KQIMLNKSPNNKELELTKEEFAKVEQVKETLMASERALDETVQEAQKVLNMVNGLNPSNK 220

Query: 249 DHVLSAYKDNAAVMEGSV 266
D VL A KD A + V
Sbjct: 221 DQVL-AKKDVAKAISNVV 237


74SO_3301SO_3308N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3301225-4.720252flavocytochrome c flavin subunit
SO_3302222-4.171342extracellular peptidase family S8A
SO_3303219-4.052005cell surface protein
SO_3305116-3.083532two component signal transduction system
SO_3306114-1.987596two component signal transduction system hybrid
SO_33082161.53300650S ribosome assembly GTPase Der
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3301PF07520310.013 Virulence protein SrfB
		>PF07520#Virulence protein SrfB

Length = 1041

Score = 31.1 bits (70), Expect = 0.013
Identities = 14/68 (20%), Positives = 29/68 (42%), Gaps = 2/68 (2%)

Query: 330 AQLAVLASGTKEKPNMPFVFCGEATANHAEGFKAAYRDGAIKKSETLEELAKRYDVDINA 389
Q+A+ + + + + +V A + F+ GA+ S L+ L D +
Sbjct: 148 VQIALDTALSDQDQSAHYVAPERADSEKPREFRLVSDPGAM--SWFLQRLEADEDGNAVD 205

Query: 390 LQNSINEW 397
LQ +++W
Sbjct: 206 LQLWVSDW 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3302SUBTILISIN1073e-27 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 107 bits (269), Expect = 3e-27
Identities = 60/278 (21%), Positives = 99/278 (35%), Gaps = 76/278 (27%)

Query: 161 KVIKETPTELQTDNGPQLIGASNLWDGNATGLAAKGDGIIIGILDTGINTDNRAFSAVGD 220
+VIK+ + G ++I A +W+ +G G+ + +LDTG + D+
Sbjct: 11 QVIKQEQQVNEIPRGVEMIQAPAVWNQT------RGRGVKVAVLDTGCDADHPDLK---- 60

Query: 221 DGHNIINPLGSGNYLGDCVKDATLCNDKLIGVYSFPLVTDEYNGLRPANGEDYNGHGSHT 280
++IG +F + + P +DYNGHG+H
Sbjct: 61 --------------------------ARIIGGRNF----TDDDEGDPEIFKDYNGHGTHV 90

Query: 281 ASTAAGNALVNVPVLMPNIGEEVGDGIETGTVLSNISGVAPHANIISYQVCDQSGCYP-S 339
A T A G + GVAP A+++ +V ++ G
Sbjct: 91 AGTIAATE--------NENG---------------VVGVAPEADLLIIKVLNKQGSGQYD 127

Query: 340 LTIASVELAIKAGVDVLNYSIGPRGGVQNDPWNTASDIAFLSAREAGIFVAMAAGNAGPD 399
I + AI+ VD+++ S+G V A A + I V AAGN G
Sbjct: 128 WIIQGIYYAIEQKVDIISMSLGGPEDV------PELHEAVKKAVASQILVMCAAGNEGDG 181

Query: 400 AETVGNV-----APWAISVAASSHQRVWSHVLS-GSGV 431
+ + ISV A + R S + + V
Sbjct: 182 DDRTDELGYPGCYNEVISVGAINFDRHASEFSNSNNEV 219



Score = 71.0 bits (174), Expect = 5e-15
Identities = 32/132 (24%), Positives = 51/132 (38%), Gaps = 33/132 (25%)

Query: 578 DILADFSSRGPYKWQTELMVPHIAAPGVDIYAAYADEMPFTSVNDAAPSDFAFLSGTSMA 637
++FS+ + APG DI + +A SGTSMA
Sbjct: 207 RHASEFSNSNNE--------VDLVAPGEDILSTVPG------------GKYATFSGTSMA 246

Query: 638 SPHVAGSAALLRQL-----HPDWTPAEIQSAMMLTATTNVLKEDGKTPAGIFDIGSGRLQ 692
+PHVAG+ AL++QL D T E+ + ++ G +P G+G L
Sbjct: 247 TPHVAGALALIKQLANASFERDLTEPELYAQLIKRTIP-----LGNSP---KMEGNGLLY 298

Query: 693 IDKAAQAGLVMD 704
+ + + D
Sbjct: 299 LTAVEELSRIFD 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3305HTHFIS493e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 49.4 bits (118), Expect = 3e-09
Identities = 26/120 (21%), Positives = 53/120 (44%), Gaps = 5/120 (4%)

Query: 6 HVMIADDHPLYLDALVNGLVSHLPGTQVSQANNYIELFDSLYLQVEEIDLLIMDLFMPGS 65
+++ADD L L G V +N L+ ++ + DL++ D+ MP
Sbjct: 5 TILVADDDAAIRTVLNQALSRA--GYDVRITSNAATLWR--WIAAGDGDLVVTDVVMPDE 60

Query: 66 SGYAGLSFLRTQFPTLPIVVISALDDLIARSQCIQHGA-AFISKSTAPTNIFKQVEQILD 124
+ + L ++ P LP++V+SA + + + + GA ++ K T + + + L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3306PF06580310.035 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 30.6 bits (69), Expect = 0.035
Identities = 15/107 (14%), Positives = 38/107 (35%), Gaps = 18/107 (16%)

Query: 935 SLLLRRVIDNILSNAIKISDPDTSVSLSVCQERQHAVIEVIDQGPGMTQQMQAELFTPFK 994
+L++ +++N + + I + L ++ +EV + G + +
Sbjct: 257 PMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK-------- 308

Query: 995 RWTSRYQGSGLGLS-VVKGIADLLG--ISLSIRSTLGEGTQFTLKLP 1038
+ +G GL V + + L G + + G + +P
Sbjct: 309 ------ESTGTGLQNVRERLQMLYGTEAQIKLSEKQG-KVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3308TCRTETOQM330.003 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 32.9 bits (75), Expect = 0.003
Identities = 38/159 (23%), Positives = 67/159 (42%), Gaps = 35/159 (22%)

Query: 199 IKLAIIGKPNVGKSTLTNRIL----GEERVVVYDEPGTTRDSIYIPMER----------- 243
I + ++ + GK+TLT +L + D+ T D+ + +R
Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSF 63

Query: 244 --DGREYVIIDTAGVRRRSKVHEVIEKFSVIKTLKAVEDANVVLLIIDAREGVAEQDLGL 301
+ + IIDT G + + E V ++L ++ A +L+I A++GV Q L
Sbjct: 64 QWENTKVNIIDTPG-----HMDFLAE---VYRSLSVLDGA---ILLISAKDGVQAQTRIL 112

Query: 302 LGFALNAGRALVIAVNKWD--GID-----QGIKDRVKSE 333
G + +NK D GID Q IK+++ +E
Sbjct: 113 FHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAE 151


75SO_3457SO_3464N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3457-213-0.810776two component signal transduction system hybrid
SO_3458-214-1.073784protein of unknown function DUF416
SO_3459-312-0.285789protein of unknown function DUF3319
SO_3460-311-0.175478transcriptional regulator of L-lactate
SO_3461-1140.108092transporter AEC family
SO_3462-1120.778526DNA repair protein RecN
SO_3463-1141.039514phosphatidylglycerophosphatase A PgpA
SO_3464-2131.951807thiamine-phosphate kinase ThiL
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3457HTHFIS641e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 64.1 bits (156), Expect = 1e-12
Identities = 27/124 (21%), Positives = 47/124 (37%), Gaps = 2/124 (1%)

Query: 680 QSLTVLAVDDNFANLKLIDTLLNELVTTVIAVNSGEEAVKQAKTRTFDLIFMDIQMPGTD 739
T+L DD+ A +++ L+ V ++ + DL+ D+ MP +
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 740 GISATKQIRQGSMNRNTPIIAVTAHAIAEERELILGSGMDGYLPKPIDEAALKAEINRWI 799
+I+ + P++ ++A G YLPKP D L I R +
Sbjct: 62 AFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 800 TRPK 803
PK
Sbjct: 120 AEPK 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3458FLAGELLIN290.010 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 29.2 bits (65), Expect = 0.010
Identities = 14/48 (29%), Positives = 22/48 (45%), Gaps = 1/48 (2%)

Query: 94 AMDAVVALSTLLGAIQTNLEEDITNISKLSSSTVANYIETISDVDLTD 141
A+ V A+ + LGAIQ + ITN+ ++ + I D D
Sbjct: 427 ALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSAR-SRIEDADYAT 473


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3462GPOSANCHOR389e-05 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 38.1 bits (88), Expect = 9e-05
Identities = 45/247 (18%), Positives = 81/247 (32%), Gaps = 19/247 (7%)

Query: 138 KSEHQLTLLDSYANHRLLIDTVTASYQRCKQIEAELKQLEASQHERIARKQLVQYQVEEL 197
SE + + A L + + A++K LEA + ARK ++ +E
Sbjct: 108 LSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA 167

Query: 198 DEFDLKVGEFEEIEQEHKRLANGTELVDSCQASLYLLTDGEESNIESLLNKVVSLAENLQ 257
+ K L +++ QA L + + + + L+
Sbjct: 168 MN------FSTADSAKIKTLEAEKAALEARQAEL----EKALEGAMNFSTADSAKIKTLE 217

Query: 258 SYDPALTNVSTMLNEALIQVQESAGELQHYLSKLELDPAYFAYLEERLSKAMQLARKHHV 317
+ AL L +AL + + LE A A LE R ++ +
Sbjct: 218 AEKAALAARKADLEKALEGAMNFSTADSAKIKTLE---AEKAALEARQAELEKALEGAMN 274

Query: 318 SPDKLAEHHLALKAELTTLDDDETKLEEIQLQVEASKSAYLANAQKLSQSRARYAK---E 374
+ L+AE L+ ++ LE Q + + + + L SR + E
Sbjct: 275 FSTADSAKIKTLEAEKAALEAEKADLEH---QSQVLNANRQSLRRDLDASREAKKQLEAE 331

Query: 375 LDKLVTQ 381
KL Q
Sbjct: 332 HQKLEEQ 338



Score = 33.9 bits (77), Expect = 0.002
Identities = 38/233 (16%), Positives = 79/233 (33%), Gaps = 7/233 (3%)

Query: 131 HAHHAMLKSEHQLTLLDSYANHRLLIDTVTASYQRCKQIEAELKQLEASQHE-RIARKQL 189
A A L++ + ++ TA + K +EAE L A + + A +
Sbjct: 182 EAEKAALEARQA----ELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA 237

Query: 190 VQYQVEELDEFDLKVGEFEEIEQEHKRLANGTELVDSCQASLYLLTDGEESNIESLLNKV 249
+ + + + E +E L E + + E+ +L +
Sbjct: 238 MNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEK 297

Query: 250 VSLAENLQSYDPALTNVSTMLNEALIQVQESAGELQHYLSKLELDPAYFAYLEERLSKAM 309
L Q + ++ L+ + ++ E Q + ++ A L L +
Sbjct: 298 ADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASR 357

Query: 310 QLARKHHVSPDKLAEHHLALKAELTTLDDDETKLEEIQLQVEASKSAYLANAQ 362
+ ++ KL E + +A +L D E + QVE + AN++
Sbjct: 358 EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEE--ANSK 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3464TYPE3IMQPROT270.025 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 27.4 bits (61), Expect = 0.025
Identities = 9/39 (23%), Positives = 16/39 (41%)

Query: 71 LSDLAAMGAEPAWMTLALTLPEVNETWLSGFSEGLFEAA 109
+ DL G + ++ L L+ + G GLF+
Sbjct: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTV 39


76SO_3483SO_3494N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3483-3120.400129HAE1 family efflux pump MFP component
SO_3484-311-0.353847HAE1 family efflux pump permease component
SO_3485-2150.118848multidrug efflux pump EmrD3
SO_3488-2140.624374transcriptional regulator AraC family
SO_3489-2131.053726diguanylate cyclase
SO_3490-1131.709339protein of unknown function DUF88
SO_3491-2122.108875cyclic di-GMP hydrolase
SO_3492-2113.096004HAE1 family efflux pump permease component MexF
SO_3493-292.106556HAE1 family efflux pump MFP component MexE
SO_3494-1111.617958transcriptional repressor of MexEF efflux pump
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3483RTXTOXIND513e-09 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 51.4 bits (123), Expect = 3e-09
Identities = 21/108 (19%), Positives = 47/108 (43%), Gaps = 3/108 (2%)

Query: 50 PLAQSVSLIGKLA-ADRAVVIAPQVTGKIKQIAVVSNQAVKKGQLLIELDDMKAQAAVAE 108
+ + GKL + R+ I P +K+I V ++V+KG +L++L + A+A +
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 109 ANAFLNDETRKLKEFEKLISRNAITQTEIDAQKASVDIARARLASAQA 156
+ L +L++ I +I ++ K + ++ +
Sbjct: 139 TQSSL--LQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEV 184



Score = 44.8 bits (106), Expect = 3e-07
Identities = 42/246 (17%), Positives = 88/246 (35%), Gaps = 33/246 (13%)

Query: 51 LAQSVSLIGKLAADRAVVIAPQVTGKIKQIAVVSNQ-----AVKKGQLLIELDDMKAQAA 105
Q + K A+R V+A ++ V ++ ++ Q + + ++ +
Sbjct: 202 KYQKELNLDKKRAERLTVLA-RINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENK 260

Query: 106 VAEANAFLNDETRKLKEFEKLISRNAITQTEIDAQ----------KASVDIA--RARLAS 153
EA L +L++ E I + + + +I LA
Sbjct: 261 YVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAK 320

Query: 154 AQADLHYHSLIAPFAGQT-GLINFSEGKMVSTGNELMTL-DDLSSMRLDLQVPEHFLSQI 211
+ + AP + + L +EG +V+T LM + + ++ + V + I
Sbjct: 321 NEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFI 380

Query: 212 SIGMPVSSTSRAWPGETF---IGKVVAIDP-RVNEETLNL--KIRVQFE-------NTDN 258
++G A+P + +GKV I+ + ++ L L + + E N +
Sbjct: 381 NVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNKNI 440

Query: 259 RLKPGM 264
L GM
Sbjct: 441 PLSSGM 446


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3484ACRIFLAVINRP7810.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 781 bits (2019), Expect = 0.0
Identities = 309/1032 (29%), Positives = 518/1032 (50%), Gaps = 28/1032 (2%)

Query: 3 LSDVSVKRPVVAIVLSLLLCVFGLVSFTKLSVREMPDVESPVVTVSTSYSGASAAIMESQ 62
+++ ++RP+ A VL+++L + G ++ +L V + P + P V+VS +Y GA A ++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 ITKTLEDELTGISGIDEITSTT-RNGSSRITVKFLLGWNLTEGVSDVRDAVARAQRRLPE 121
+T+ +E + GI + ++ST+ GS IT+ F G + V++ + A LP+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 DANDPVVSKDNGSGEPSVYVNLSSSVMDRTQ--LTDYAQRVLEDRFSLISGVSSISISGG 179
+ +S + S + S TQ ++DY ++D S ++GV + + G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 180 LYKVMYVKLRPEQMAGRNVTVTDITSALRKENIETPGGQVRNDTTV------MSVRTKRL 233
Y + + L + + +T D+ + L+ +N + GQ+ + S+ +
Sbjct: 181 QYAMR-IWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 234 YYTPKDFDYLVVRTASDGTPIYLKDVADVAVGAQNENSTFKSDGIVNLSLGVITQSDANP 293
+ P++F + +R SDG+ + LKDVA V +G +N N + +G LG+ + AN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 LVVAQEVHKEVDKIQDFLPEGTSLVVDFDSTVFIDRSINEVYNTLYVTGALVVLVLYIFI 353
L A+ + ++ ++Q F P+G ++ +D+T F+ SI+EV TL+ LV LV+Y+F+
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 GQVRATLIPAVTVPVSLISAFIAANMFGYSINLLTLMALILAIGLVVDDAIVVVENIFHH 413
+RATLIP + VPV L+ F FGYSIN LT+ ++LAIGL+VDDAIVVVEN+
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 I-EKGEEPLLAAYKGTREVGFAVVATTAVLVMVFLPISFMEGMVGLLFTEFSVMLAVSVL 472
+ E P A K ++ A+V VL VF+P++F G G ++ +FS+ + ++
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 FSSLIALTLTPVLSSKLLKANVK-----PNRFNRFVDRGFARMEKLYHVGVTHAIRFRLL 527
S L+AL LTP L + LLK F + + F Y V +
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 528 APLVILACIGGSVWLMQQVPAQLAPQEDRGVLYAFVKGAEGTSYNRMTANMDIVEDRLMP 587
L+ + G V L ++P+ P+ED+GV ++ G + R +D V D +
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 588 LLGQGVLRSFSVQAPAFGGRAGDQTGYVIMQLEDWEHRNVTAQQALGIISKA---LKDIP 644
V F+V +F G+ G + L+ WE RN A +I +A L I
Sbjct: 600 NEKANVESVFTVNGFSFSGQ-AQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 645 DVMVRPM-MPGFRGQ-SSEPVQFVL---GGSDYAELFKWAQVLKEQANASP-MMEGADLD 698
D V P MP ++ F L G + L + L A P + +
Sbjct: 659 DGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 699 YAETTPELIVTVDKERAAELGISVDDVSQTLEVMLGGRKETTYVDRGEEYDVYLRGDENS 758
E T + + VD+E+A LG+S+ D++QT+ LGG ++DRG +Y++ D
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 759 FNNVGDLSQIYMRSAKGELVTLDTVTHIEEVASAQRLSHTNKQKSITLKANISKGYTLGE 818
D+ ++Y+RSA GE+V T V + RL N S+ ++ + G + G+
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 819 ALTFLENKSIELLPKDISIGYTGESKDFKENQSSIFIVFGLALLVAYLVLAAQFESFINP 878
A+ +EN + + LP I +TG S + + + + ++ +V +L LAA +ES+ P
Sbjct: 839 AMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIP 897

Query: 879 LVVMFTVPMGVFGGFLGLLVTSQGINIYSQIGMIMLIGMVTKNGILIVEFANQLRDR-GL 937
+ VM VP+G+ G L + +Q ++Y +G++ IG+ KN ILIVEFA L ++ G
Sbjct: 898 VSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGK 957

Query: 938 ALDKAIIDASTRRLRPILMTAFTTLVGAIPLIFSTGAGSESRIAVGTVVFFGMAFATFVT 997
+ +A + A RLRPILMT+ ++G +PL S GAGS ++ AVG V GM AT +
Sbjct: 958 GVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLA 1017

Query: 998 LFVIPAMYRLIS 1009
+F +P + +I
Sbjct: 1018 IFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3485TCRTETB604e-12 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 60.3 bits (146), Expect = 4e-12
Identities = 60/263 (22%), Positives = 114/263 (43%), Gaps = 26/263 (9%)

Query: 7 LWLAVLLMMFPQIMETIYSPALPDIAEHFAVPISAAAQTLSVYFVAFAIGVFCWGRLADV 66
+WL +L + E + + +LPDIA F P ++ + + + F+IG +G+L+D
Sbjct: 17 IWLCILSFFSV-LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 67 IGRRKAMLAGLVCYAIGSAFALLV-SDFSLLLMARVLSAFGAA----VGSVITQTMMRDS 121
+G ++ +L G++ GS + S FSLL+MAR + GAA + V+ +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 122 YSGEELAKVFSVMGMSFGISPVIGLLLGSVLSAFWGYQGVFVVLMSSAIVLLFLSVKSLP 181
G+ + S++ M G+ P IG ++ + W Y +++ I+ + +K L
Sbjct: 136 NRGKAFGLIGSIVAMGEGVGPAIGGMIAHYI--HWSY---LLLIPMITIITVPFLMKLLK 190

Query: 182 ETKPAHTQTIAIGELAIKMLSDRGIIKNTLLVAAFNLMWFSYFSLAPFMFEAQ------- 234
+ I + + + GI+ L ++++ + L+ +F
Sbjct: 191 KEV-RIKGHFDIKGIILMSV---GIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDP 246

Query: 235 ----GLSTLIFGMSGLLLGFGAF 253
GL I M G+L G F
Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIF 269


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3492ACRIFLAVINRP10320.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1032 bits (2670), Expect = 0.0
Identities = 420/1043 (40%), Positives = 639/1043 (61%), Gaps = 18/1043 (1%)

Query: 2 LSQFFIKRPIFAAVLSLLFFITGAIAVWQLPITEYPEVVPPTVVVTANYPGANPKVIAET 61
++ FFI+RPIFA VL+++ + GA+A+ QLP+ +YP + PP V V+ANYPGA+ + + +T
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 62 VASPLEQEINGVEDMLYMSSQATSDGRMTLTITFAIGTDVDRAQTQVQSRVDRAMPRLPQ 121
V +EQ +NG+++++YMSS + S G +T+T+TF GTD D AQ QVQ+++ A P LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 EVQRLGIVTEKSSPDLTMVVHLLSPDNRYDMLYLSNYAALNVKDELARIKGVGAVRLFGA 181
EVQ+ GI EKSS MV +S + +S+Y A NVKD L+R+ GVG V+LFG
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG- 179

Query: 182 GEYSLRIWLDPNKVSALGMSPSDIIAAVREQNQQAAAGSLGAQPSGNA-DFQLLINVKGR 240
+Y++RIWLD + ++ ++P D+I ++ QN Q AAG LG P+ I + R
Sbjct: 180 AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 241 LTELSEFEDIIIKVGQNGEVIRLKDVARVELGAISYALRSLLDNKDAVAIPVFQASGSNA 300
EF + ++V +G V+RLKDVARVELG +Y + + ++ K A + + A+G+NA
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 301 IQISDDVRAEMARLAKSFPEGLQYEIVYDPTVFVRGSIEAVVKTLLEAVLLVVLVVVLFL 360
+ + ++A++A L FP+G++ YD T FV+ SI VVKTL EA++LV LV+ LFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 361 QTWRASIIPLVAVPVSLVGTFAFMHLMGFSLNALSLFGLVLAIGIVVDDAIVVVENVERN 420
Q RA++IP +AVPV L+GTFA + G+S+N L++FG+VLAIG++VDDAIVVVENVER
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 421 IAS-GLSPIAATQKAMKEVTGPIVATTLVLAAVFIPTAFMSGLTGQFYKQFALTITISTF 479
+ L P AT+K+M ++ G +V +VL+AVFIP AF G TG Y+QF++TI +
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 480 ISAINSLTLSPALSALLLKGHDAPKDALTRLMDKLFGGWLFTPFNRLFNRASEGYGYLVR 539
+S + +L L+PAL A LLK A G F FN F+ + Y V
Sbjct: 480 LSVLVALILTPALCATLLKPVSAE--------HHENKGGFFGWFNTTFDHSVNHYTNSVG 531

Query: 540 KVIRFGGIIGLVYLGMVALTGVQFVNTPTGYVPGQDKQYLVAFAQLPDAASLERTDVVIK 599
K++ G L+Y +VA V F+ P+ ++P +D+ + QLP A+ ERT V+
Sbjct: 532 KILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLD 591

Query: 600 KMSDIALNH--PGVAHSIAFPGLSINGFTNSPNSGVVFVALDDFELRKSPELSANAIAGQ 657
+++D L + V G S +G + N+G+ FV+L +E R E SA A+ +
Sbjct: 592 QVTDYYLKNEKANVESVFTVNGFSFSG--QAQNAGMAFVSLKPWEERNGDENSAEAVIHR 649

Query: 658 LNQQFAGIQDAFIAIFPPPPVQGLGTIGGFRLQIQDRANLGYEALYQVTQQVMYKAWADP 717
+ I+D F+ F P + LGT GF ++ D+A LG++AL Q Q++ A P
Sbjct: 650 AKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHP 709

Query: 718 -QLAGIFSSYQVNVPQLELDIDRTKAKQQAVSLDQIFQTLQTYMGSTYVNDFNRFGRTYQ 776
L + + + Q +L++D+ KA+ VSL I QT+ T +G TYVNDF GR +
Sbjct: 710 ASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKK 769

Query: 777 VNMQADEAFRQSPQQISQLKVPNVNGDMIPLGSFINVSQSAGPDRVMHYNGFTTAEINGG 836
+ +QAD FR P+ + +L V + NG+M+P +F G R+ YNG + EI G
Sbjct: 770 LYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGE 829

Query: 837 PAPGVSSGQAQAAIEKILAETLPIGMTYEWTELTYQQILAGNTGLLVFPLVIVLVFMVLA 896
APG SSG A A +E + ++ LP G+ Y+WT ++YQ+ L+GN + + V+VF+ LA
Sbjct: 830 AAPGTSSGDAMALMENLASK-LPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLA 888

Query: 897 AQYESLSLPLAIILIIPMTLLSALSGVLLYGGDNNIFTQIGLIVLVGLATKNAILIVEFA 956
A YES S+P++++L++P+ ++ L L+ N+++ +GL+ +GL+ KNAILIVEFA
Sbjct: 889 ALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFA 948

Query: 957 KEKQDH-GMEVMESILEAARLRLRPILMTSIAFIMGVVPMVFSTGAGAEMRQAMGVAVFA 1015
K+ + G V+E+ L A R+RLRPILMTS+AFI+GV+P+ S GAG+ + A+G+ V
Sbjct: 949 KDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMG 1008

Query: 1016 GMIGVTIFGLILTPLFYYSLVKR 1038
GM+ T+ + P+F+ + +
Sbjct: 1009 GMVSATLLAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3493RTXTOXIND462e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 46.0 bits (109), Expect = 2e-07
Identities = 34/181 (18%), Positives = 68/181 (37%), Gaps = 27/181 (14%)

Query: 6 TLRTLMLTTVAAFVLSACGEPEVLQGQAPAAPKVDVAQVLQERVTEWDEFTGRLQAPESV 65
+M V AF+LS G +V++ ++T +GR S
Sbjct: 60 VAYFIMGFLVIAFILSVLG-------------QVEIVATANGKLT----HSGR-----SK 97

Query: 66 TLVPRVSGYIASVNFKEGALVKKGDVLFRIDASVFEAEVARLKADLASALSA---DQLAT 122
+ P + + + KEG V+KGDVL ++ A EA+ + ++ L A Q+ +
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 123 NDLERARKLFVQKAVSAELLDTRESNKRQTTAAVASVKAALLR--AELDLDYTQVRAPID 180
+E + ++ + E + T+ + + + +L+ + RA
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 181 G 181

Sbjct: 218 T 218



Score = 39.0 bits (91), Expect = 2e-05
Identities = 21/102 (20%), Positives = 41/102 (40%), Gaps = 10/102 (9%)

Query: 101 EAEVARLKADLASALSADQLATNDLERARKLFVQKAVSAELLDTRESNKRQTTAAVASVK 160
E+ K+ L S A + + +LF + + +L RQTT + +
Sbjct: 265 VNELRVYKSQLEQIESEILSAKEEYQLVTQLF-KNEILDKL--------RQTTDNIGLLT 315

Query: 161 AALLRAELDLDYTQVRAPIDGRASYANV-TAGNYVSAGQSVL 201
L + E + +RAP+ + V T G V+ ++++
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLM 357


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3494HTHTETR587e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.5 bits (141), Expect = 7e-13
Identities = 35/160 (21%), Positives = 61/160 (38%), Gaps = 5/160 (3%)

Query: 23 LAKALEVFWRKGFEGTSLTDLTQAMGINKPSLYAAFGNKEQLFLKAIELYEQRPCAFFYP 82
L AL +F ++G TSL ++ +A G+ + ++Y F +K LF + EL E
Sbjct: 17 LDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELE 76

Query: 83 SLEK--ETAYQVVESMLLGAATSLVDENQPHGCLIVQGALACSEAGQAIKETLITRRRDG 140
K V+ +L+ S V E + + + C G+ R
Sbjct: 77 YQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHK-CEFVGEMAVVQQAQRNLCL 135

Query: 141 E--QALCERLQRAKDEGDLPADADPLLLARYVGTVLQGMA 178
E + + L+ + LPAD A + + G+
Sbjct: 136 ESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLM 175


77SO_3602SO_3615N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3602-1140.477313ABC-type sulfate/thiosulfate uptake system
SO_3604-1130.370340ISSod4 transposase TnpA_ISSod4
SO_3607-1161.863670ISSod1 transposase TnpA_ISSod1
SO_36090162.115062ISSod1 transposase TnpA_ISSod1
SO_36110162.020138AAA ATPase family protein
SO_36130151.481562phosphoribosylglycinamide formyltransferase 2
SO_3614-1130.200717protein of unknown function DUF3142
SO_3615-213-0.663037TPR repeat-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3602PF05272300.021 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.021
Identities = 11/34 (32%), Positives = 16/34 (47%)

Query: 30 MIGLLGPSGSGKTTLLRIIAGLEGADSGHIHFGN 63
+ L G G GK+TL+ + GL+ H G
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGT 631


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3613PF06057310.005 Type IV secretory pathway VirJ component
		>PF06057#Type IV secretory pathway VirJ component

Length = 243

Score = 31.3 bits (71), Expect = 0.005
Identities = 10/28 (35%), Positives = 13/28 (46%), Gaps = 2/28 (7%)

Query: 17 GCGELGKEVAIELQRLGVEVIGVD--RY 42
G L K V LQ+ G V+G +Y
Sbjct: 62 GWATLDKAVGGILQQQGWPVVGWSSLKY 89


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3614BCTERIALGSPC290.036 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 29.2 bits (65), Expect = 0.036
Identities = 30/137 (21%), Positives = 50/137 (36%), Gaps = 19/137 (13%)

Query: 322 PEVLQSFVKKLHTLADPNLRGMIWFRLPLEGDKRMWPLSTLIAVAKQQPLAPQVELQMVS 381
P V++ + L L MI++R+ L + + + A A+QQP+
Sbjct: 11 PSVIRRILFYLLMLLFCQQLAMIFWRIGLPDNAPVSSVQITPAQARQQPVTL-------- 62

Query: 382 QPMTELAQEPAPNAKQTDQQTKANKPPQTTLFQLVLVNKGNLAGKVPSQLSLAAQSCSGY 441
T P N ++ + P + L L G +AG S+ S+A S
Sbjct: 63 NDFTLFGVSPEKNKAGALDASQMSNLPPS---TLNLSLTGVMAGDDDSR-SIAIISKDNE 118

Query: 442 -------DAQNGYIAKL 451
+ GY AK+
Sbjct: 119 QFSRGVNEEVPGYNAKI 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3615SYCDCHAPRONE300.020 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 29.9 bits (67), Expect = 0.020
Identities = 32/174 (18%), Positives = 65/174 (37%), Gaps = 28/174 (16%)

Query: 103 ENSELSEAQLVLVNSLRDAPSLAQAEQIAAQLKDTLPPALTWYSLGAMAFDAKEYDKASN 162
E ++ E QL + + L+ ++A +I++ + L YSL + + +Y+ A
Sbjct: 4 ETTDTQEYQLAMESFLKGGGTIAMLNEISSDTLEQL------YSLAFNQYQSGKYEDAHK 57

Query: 163 YFKKVIVLPAS------------ERAGRSLWALYSLSRIELLQSK-PSPDNAHFAKANAY 209
F+ + VL + G+ A++S S ++ K P F A
Sbjct: 58 VFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRF---PFHAAECL 114

Query: 210 LMQLQAEVIQGAADPLRLSLA------SLGEQAYILLHQGQPSIEIAHEEYEAP 257
L + + + + +A L + +L + E+ HE + P
Sbjct: 115 LQKGELAEAESGLFLAQELIADKTEFKELSTRVSSMLEAIKLKKEMEHECVDNP 168


78SO_3969SO_3988N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_3969-113-1.052935putative outer membrane lipoprotein YiaD
SO_3972012-1.997349transposase domain protein
SO_3973-112-1.900554Ser/Thr protein kinase
SO_3974012-2.465688lipoprotein of unknown function DUF2799
SO_3975011-1.955092membrane protein PAP2 superfamily
SO_398009-1.240857ammonia-forming nitrite reductase NrfA
SO_3981-190.088659nitrate/nitrite-responsive two component signal
SO_3982-2110.524801nitrate/nitrite-responsive two component signal
SO_3983-2130.638760protein of unknown function UPF0231
SO_3984-2131.247116metal ion transporter MIT family
SO_3985-2161.786875dihydrodipicolinate synthase DapA
SO_3986-111-0.831815lysine-sensitive aspartokinase III LysC
SO_3987112-4.268909protein of unknown function DUF3293
SO_3988012-4.219851two component signal transduction system
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3969OMPADOMAIN974e-26 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 97.3 bits (242), Expect = 4e-26
Identities = 37/123 (30%), Positives = 62/123 (50%), Gaps = 11/123 (8%)

Query: 116 LNMPNEVTFGVDQTELSDGAKRVLNSVALVAKEYSKT--QLNVLGYTDSSGSDSYNLRLS 173
+ ++V F ++ L + L+ + + VLGYTD GSD+YN LS
Sbjct: 215 FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS 274

Query: 174 QVRASEVGNYLMSKGVASARVKSKGMGEASPIASNANAEGR---------AQNRRVEIVL 224
+ RA V +YL+SKG+ + ++ ++GMGE++P+ N + A +RRVEI +
Sbjct: 275 ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 225 TPT 227

Sbjct: 335 KGI 337


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3972PF04183260.014 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 26.0 bits (57), Expect = 0.014
Identities = 8/39 (20%), Positives = 15/39 (38%), Gaps = 3/39 (7%)

Query: 5 NHSLKPDVIFEVKVKGRLLSDVARQYGLSAKSVYQWVRE 43
H L+ V R +S + + G+ + YQ +
Sbjct: 477 IHDLQTGHFVTV---LRFISPLMVRLGVPERRFYQLLAA 512


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3981PF06580441e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 43.7 bits (103), Expect = 1e-06
Identities = 27/147 (18%), Positives = 56/147 (38%), Gaps = 17/147 (11%)

Query: 410 INEGVSTAYVQLRELLSTFRLTIKEPNLKN-AMEAMLEQLRANTDI-------KIHLDYK 461
I E + A L L R +++ N + ++ L + + + ++ + +
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQ 245

Query: 462 LSPQWLEAKQHIHILQITREATLNAIKHA-----NASHINIRCYKDDRGMVNISVSDNGV 516
++P ++ + ++Q E N IKH I ++ KD+ G V + V + G
Sbjct: 246 INPAIMDVQVPPMLVQTLVE---NGIKHGIAQLPQGGKILLKGTKDN-GTVTLEVENTGS 301

Query: 517 GIGHIKERDQHFGIGIMHERASKLDGE 543
+ G+ + ER L G
Sbjct: 302 LALKNTKESTGTGLQNVRERLQMLYGT 328


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3982HTHFIS607e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 59.8 bits (145), Expect = 7e-13
Identities = 26/159 (16%), Positives = 64/159 (40%), Gaps = 9/159 (5%)

Query: 6 SVLVVDDHPLLRKGICQLITSDPDFSLFGEVGSGLDALSSVATDEPDIVLLDLNMKGMTG 65
++LV DD +R + Q ++ + + + +A + D+V+ D+ M
Sbjct: 5 TILVADDDAAIRTVLNQALS-RAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 66 LDTLNAMRQEGVTSRIVILTVSDAKQDVIRLLRAGADGYLLKDTEPDLLLDKLKNTMSGH 125
D L +++ +++++ + I+ GA YL K + L+ +
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG------ 116

Query: 126 RVISEEVAEYLYELKNAADEQEWVSSLTPRELQILQQLA 164
R ++E +L++ + + + + +I + LA
Sbjct: 117 RALAEPKRR-PSKLEDDSQDGMPLVGRSAAMQEIYRVLA 154


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3984ACRIFLAVINRP300.016 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.8 bits (67), Expect = 0.016
Identities = 19/87 (21%), Positives = 37/87 (42%), Gaps = 9/87 (10%)

Query: 229 IRTIEDLDALRDRANVTQEELLSQQSEQLNKRLYFLSLV-SVIFLPLGFLTGLLGVNIGG 287
I +E+++ + + +E + Q+ L +++V S +F+P+ F G G
Sbjct: 410 IVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIY-- 467

Query: 288 IPGADNNFAFT-SFCIILVSLVALQMV 313
F+ T + L LVAL +
Sbjct: 468 -----RQFSITIVSAMALSVLVALILT 489


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3986CARBMTKINASE310.011 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 30.6 bits (69), Expect = 0.011
Identities = 18/81 (22%), Positives = 27/81 (33%), Gaps = 5/81 (6%)

Query: 202 DYSAALLAEALTASAVEIWTDVAGIYTTDPRLAPNAHPIAEISFNEAAEMATFGAKVLHP 261
D + LAE + A I TDV G + E+ E + G
Sbjct: 216 DLAGEKLAEEVNADIFMILTDVNGAALY--YGTEKEQWLREVKVEELRKYYEEGH--FKA 271

Query: 262 ATILPAVRQQIQVFVGSSKEP 282
++ P V I+ F+ E
Sbjct: 272 GSMGPKVLAAIR-FIEWGGER 291


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_3988HTHFIS832e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 82.6 bits (204), Expect = 2e-20
Identities = 31/131 (23%), Positives = 61/131 (46%), Gaps = 1/131 (0%)

Query: 1 MQNPHILIVEDEAVTRNTLRSIFEAEGYVVTEANDGAEMHKAMQENKINLVVMDINLPGK 60
M IL+ +D+A R L GY V ++ A + + + +LVV D+ +P +
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 NGLLLARELREIN-NIGLIFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLT 119
N L +++ ++ ++ ++ ++ + I E GA DY+ KPF+ EL L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 120 RVNSAGNEVEE 130
+++E+
Sbjct: 121 EPKRRPSKLED 131


79SO_4097SO_4112N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_40970150.609654cell wall structural complex component MreC
SO_4098117-0.160643cell wall structural complex component MreB
SO_4100120-1.033251MshA pili-associated adhesin MshQ
SO_4101221-1.017663MSHA secretion apparatus component MshP
SO_4102221-1.612463MshA minor pilin protein MshO
SO_4103218-1.093877MshA minor pilin protein MshD
SO_4104017-0.229679MSHA minor pilin protein MshC
SO_4105-2140.505750MSHA major pilin subunit MshA
SO_4106-2141.390891MSHA minor pilin protein MshB
SO_4107-2141.427377MshA biogenesis protein MshF
SO_4108-2151.548207MSHA biogenesis protein MshG
SO_4109-2151.257285MSHA pilus assembly ATPase MshE
SO_4110-2161.161587MSHA biogenesis protein MshN
SO_4111-2160.449573MSHA biogenesis protein MshM
SO_4112-316-0.361093MSHA system outer membrane secretin MshL
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4097PF05616290.030 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 28.9 bits (64), Expect = 0.030
Identities = 33/120 (27%), Positives = 46/120 (38%), Gaps = 26/120 (21%)

Query: 229 GYPVARVMKVFTEDGQSYTRVTAQPLAALDRIRYLLLIWPSPD---SGVTLPNQIPVP-- 283
G PV +V+ F D Q T V Q + P PD PN P+P
Sbjct: 286 GNPV-QVVATFGRDSQGNTTVDVQ-------------VIPRPDLTPGSAEAPNAQPLPEV 331

Query: 284 -AADHPVEEAKPDAVNGA-INPQGEAQGTNATVPNATVPNTTAPNATVPNATAAPRNANG 341
A++P P+ G NP+ + P+A P+T T P++ A P NG
Sbjct: 332 SPAENPANNPAPNENPGTRPNPEPDPDLN----PDAN-PDTDGQPGTRPDSPAVPDRPNG 386


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4098SHAPEPROTEIN5570.0 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 557 bits (1438), Expect = 0.0
Identities = 314/348 (90%), Positives = 332/348 (95%), Gaps = 1/348 (0%)

Query: 1 MFKKLRGIFSNDLSIDLGTANTLIYVRDEGIVLNEPSVVAIRGERNSSGQKSVAAVGTEA 60
M KK RG+FSNDLSIDLGTANTLIYV+ +GIVLNEPSVVAIR +R + KSVAAVG +A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDR-AGSPKSVAAVGHDA 59

Query: 61 KQMLGRTPGNIQAIRPMKDGVIADFYVTEKMLQHFIKQVHNNSFFRPSPRVLVCVPVGAT 120
KQMLGRTPGNI AIRPMKDGVIADF+VTEKMLQHFIKQVH+NSF RPSPRVLVCVPVGAT
Sbjct: 60 KQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGAT 119

Query: 121 QVERRAIRESAMGAGAREVYLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAIISLN 180
QVERRAIRESA GAGAREV+LIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVA+ISLN
Sbjct: 120 QVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN 179

Query: 181 GVVYSSSVRIGGDKFDDAIINYVRRNYGSLIGEATAERIKHTIGTAYPGDEVLEIEVRGR 240
GVVYSSSVRIGGD+FD+AIINYVRRNYGSLIGEATAERIKH IG+AYPGDEV EIEVRGR
Sbjct: 180 GVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGR 239

Query: 241 NLAEGVPRSFTLNSNEILEALQEPLSGIVSAVMVALEQSPPELASDISERGMVLTGGGAL 300
NLAEGVPR FTLNSNEILEALQEPL+GIVSAVMVALEQ PPELASDISERGMVLTGGGAL
Sbjct: 240 NLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL 299

Query: 301 LRDLDRLLMQETGIPVMVADDPLTCVARGGGKALEMIDMHGGDLFSEE 348
LR+LDRLLM+ETGIPV+VA+DPLTCVARGGGKALEMIDMHGGDLFSEE
Sbjct: 300 LRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSEE 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4102BCTERIALGSPG310.002 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 31.0 bits (70), Expect = 0.002
Identities = 11/24 (45%), Positives = 17/24 (70%)

Query: 8 RKHQCSRGFTLVEMVTAILILGIL 31
R RGFTL+E++ I+I+G+L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVL 25


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4103BCTERIALGSPH393e-06 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 38.8 bits (90), Expect = 3e-06
Identities = 16/59 (27%), Positives = 33/59 (55%), Gaps = 4/59 (6%)

Query: 25 IRQQGFTLIELVVGMLVIAIAIVM-LSSMLFPQADRAAKTLHRVKSA-ELA--HSVMNE 79
+RQ+GFTL+E+++ +L++ ++ M L + + D AA+TL R ++ +
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTG 59


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4104BCTERIALGSPH415e-07 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 40.7 bits (95), Expect = 5e-07
Identities = 26/82 (31%), Positives = 43/82 (52%), Gaps = 1/82 (1%)

Query: 3 KQAGFTLVELVTTIILIGILSVTVLPRLFSQSSYSAFSLRNEFMAELRQVQQKALNNTDR 62
+Q GFTL+E++ ++L+G+ + VL + SA F A+LR VQQ+ L +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQT-GQ 60

Query: 63 CFRVAVSTTGYQVSQFASRNSA 84
F V+V +Q +R+ A
Sbjct: 61 FFGVSVHPDRWQFLVLEARDGA 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4105BCTERIALGSPG502e-10 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 49.9 bits (119), Expect = 2e-10
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 4/53 (7%)

Query: 1 MKRQQGFTLIELVVVIIILGILAVTAAPKFINLQGDARA----SALQGLKGAI 49
+Q+GFTL+E++VVI+I+G+LA P + + A S + L+ A+
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENAL 56


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4106BCTERIALGSPG438e-08 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 43.3 bits (102), Expect = 8e-08
Identities = 15/36 (41%), Positives = 27/36 (75%)

Query: 4 KQNGFSLIELVIVIVILGLLAATAIPRFLNVTDDAE 39
KQ GF+L+E+++VIVI+G+LA+ +P + + A+
Sbjct: 6 KQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKAD 41


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4108BCTERIALGSPF306e-103 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 306 bits (786), Expect = e-103
Identities = 113/406 (27%), Positives = 201/406 (49%), Gaps = 4/406 (0%)

Query: 1 MPVYQYRGRSGQGQAVTGQLDAASESAAADMLLSRGIIPLEVKAAKVAK----SFSLTQL 56
M Y Y+ QG+ G +A S A +L RG++PL V + + S L+
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 57 FGSKVGLDELQIFTRQMYSLTRSGIPILRAIAGLSETAHSQRMKDALNDISEQLTAGRPL 116
++ +L + TRQ+ +L + +P+ A+ +++ + + + + ++ G L
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 117 SSAMNQHPDVFDSLFVSMVHVGENTGKLEDAFIQLSGYIEREQETRRRIKAAMRYPIFVL 176
+ AM P F+ L+ +MV GE +G L+ +L+ Y E+ Q+ R RI+ AM YP +
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 177 IAIGLAMVILNIMVIPKFAEMFSRFGADLPWATKVLIGTSNLFVNYWPLMLVMLLGAIIG 236
+ + IL +V+PK E F LP +T+VL+G S+ + P ML+ LL +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 237 IRYWHHTEKGEKQWDKWKLHIPAVGSIIERSTLARYCRSFSMMLSAGVPMTQALSLVADA 296
R EK + + LH+P +G I ARY R+ S++ ++ VP+ QA+ + D
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 297 VDNAYMHDKIVGMRRGIESGESMLRVSNQSKLFTPLVLQMVAVGEETGQIDQLLNDAADF 356
+ N Y ++ + G S+ + Q+ LF P++ M+A GE +G++D +L AAD
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 357 YEGEVDYDLKNLTAKLEPILIGIVAVIVLILALGIYLPMWDMLNVV 402
+ E + EP+L+ +A +VL + L I P+ + ++
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLM 406


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4112BCTERIALGSPD1862e-53 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 186 bits (473), Expect = 2e-53
Identities = 78/318 (24%), Positives = 144/318 (45%), Gaps = 28/318 (8%)

Query: 236 ELKETLTAIVGDTGGGRQVVVT--PQAGLVTIRAYPNELRQVRAFLSSAESHLQRQVILE 293
++ A + +++ Q + + A P+ + + ++ + + QV++E
Sbjct: 292 TMQSEKQAAKPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIR-RPQVLVE 350

Query: 294 AKILEVTLSDGYQQGIQWDNVLGHV---GNTNINFGTSAGTGLS----DKITSSLGGVTS 346
A I EV +DG GIQW N + N+ + T+ ++SSL S
Sbjct: 351 AIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALS 410

Query: 347 ------LSIKGSDFNTMISLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSST 400
++ +++ L + D+L++P + +N +A VG + +T S
Sbjct: 411 SFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLT--GSQ 468

Query: 401 TVAGTTPVTTPQVELTPFFSGIALDVTPQIDKDGNVLLHVHPSVIDVKEQTKDIKVSNES 460
T +G T + + GI L V PQI++ +VLL + V V + S+ S
Sbjct: 469 TTSGDNIFNTVERKTV----GIKLKVKPQINEGDSVLLEIEQEVSSVADAA-----SSTS 519

Query: 461 LELPLAQSEIRESDTVIRAASGDVVVIGGLMKSENIEVVSQVPLLGDIPFVGELFKNRSK 520
+L + R + + SG+ VV+GGL+ + +VPLLGDIP +G LF++ SK
Sbjct: 520 SDLGATFN-TRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSK 578

Query: 521 QKKKTELIIMLKPTVVGN 538
+ K L++ ++PTV+ +
Sbjct: 579 KVSKRNLMLFIRPTVIRD 596


80SO_4170SO_4178N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_41700182.565140cell-cell signaling protein C-factor
SO_41710182.775504hypothetical protein
SO_4172-1172.772691oxygen-responsive two component signal
SO_4173-1172.041382oxygen-responsive two component signal
SO_4174-2160.237210dTDP-4-dehydrorhamnose reductase family
SO_4176-215-0.148219metallophosphatase binding domain protein
SO_4177-219-0.825785periplasmic protein of expolysaccharide
SO_4178-216-1.070592membrane anchored protein MxdC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4170DHBDHDRGNASE442e-07 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 43.5 bits (102), Expect = 2e-07
Identities = 38/195 (19%), Positives = 74/195 (37%), Gaps = 29/195 (14%)

Query: 55 LEEEIKQLSQNIPQLDWLINCIGMLHTEDKGPEKSLQTLDGDFFLDNIKLNTLPSMLLAK 114
++E ++ + + +D L+N G+L G SL + + +N+ ++
Sbjct: 72 IDEITARIEREMGPIDILVNVAGVLRP---GLIHSLSDEE---WEATFSVNSTGVFNASR 125

Query: 115 HFSHALKQSNSARFAVISARVGSISDNRLGGWYSYRASKAALNMFLKTLSIEWQRSIKHC 174
S + S + + + + +Y +SKAA MF K L +E C
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMA---AYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 175 VVLSLHPGTTDTPLSKP------------------FQQSVPKDKLFTPEYVAQCLVGIIA 216
++S PG+T+T + F+ +P KL P +A ++ +++
Sbjct: 183 NIVS--PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240

Query: 217 NATPTQTGTFLAYDG 231
T L DG
Sbjct: 241 GQAGHITMHNLCVDG 255


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4172HTHFIS871e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 87.2 bits (216), Expect = 1e-22
Identities = 26/129 (20%), Positives = 63/129 (48%)

Query: 3 RLLIIEDDQALAGILARRLTRHGFECRLSHDASSALLVARECCPTHILLDMKLAEANGLS 62
+L+ +DD A+ +L + L+R G++ R++ +A++ ++ D+ + + N
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 LIVPLRNLLPKVVMVLLTGYASIATAVEAIRLGADNYLAKPVDTQTLLAAFELDVHSNNL 122
L+ ++ P + +++++ + TA++A GA +YL KP D L+ +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 123 QEDDVDDSP 131
+ ++D
Sbjct: 125 RPSKLEDDS 133



Score = 47.5 bits (113), Expect = 6e-09
Identities = 13/39 (33%), Positives = 22/39 (56%)

Query: 135 KRLEWEHIQQVLNANQGNVSATARQLGMHRRTLQRKLLK 173
+E+ I L A +GN A LG++R TL++K+ +
Sbjct: 434 AEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4174NUCEPIMERASE684e-15 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 67.9 bits (166), Expect = 4e-15
Identities = 37/158 (23%), Positives = 61/158 (38%), Gaps = 20/158 (12%)

Query: 3 NIMVTGATGLLGRAVVKQLTAAGHRVI---------------ATGFSCAEAN--IHKLDL 45
+VTGA G +G V K+L AGH+V+ A A+ HK+DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 46 TQAIAVEAFIARERPEVIVHCAAERRPDVSEQSPEQALALNLSASQTLAKAAKQHG-AWL 104
+ A E + S ++P NL+ + + + + L
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 105 LYISTDYVF-DGTTPPYAE-DAAPNPVNFYGESKLQGE 140
LY S+ V+ P++ D+ +PV+ Y +K E
Sbjct: 122 LYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANE 159


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4178RTXTOXIND452e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 45.2 bits (107), Expect = 2e-07
Identities = 37/202 (18%), Positives = 65/202 (32%), Gaps = 33/202 (16%)

Query: 35 RWYLLLTLVIAPVVVVG--WILLRP-HLFILASGIVTT--EPLEVRAPSAGDVAAIMVKR 89
R L+ I +V+ +L + A+G +T E++ V I+VK
Sbjct: 55 RRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKE 114

Query: 90 GDVLASGANILTLVDTQLGAQIQELEKQLSQLEFDHLSLNAEILTQLQQRIAVAAEGVTR 149
G+ + G +L L A + + L LQ R+ R
Sbjct: 115 GESVRKGDVLLKLTALGAEADTLKTQSSL-----------------LQARLEQT-----R 152

Query: 150 QDGLLDSFERYQRQGVVPTAD--MAAVLQAHTASKMALEQAKVDLMQARQGQKTELLAGA 207
L S E + + + V + +L + + Q ++ QK L
Sbjct: 153 YQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKK 212

Query: 208 IAQSKYNIELQLARLKAQESQL 229
A+ LAR+ E+
Sbjct: 213 RAE----RLTVLARINRYENLS 230


81SO_4321SO_4329N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4321-210-2.013613Pal-like T1SS-linked outer membrane lipoprotein
SO_4322-110-1.843463T1SS associated periplasmic
SO_4323-210-0.719438bifunctional diguanylate
SO_4324-211-0.115153****bifunctional diguanylate
SO_4325-2130.756004ATP-dependent DNA helicase Rep
SO_4326-2150.444585transcriptional repressor of multidrug/detergent
SO_4327-3150.814217RND-type multidrug/detergent efflux system MFP
SO_4328-2130.771469RND-type multidrug/detergent efflux system
SO_4329-119-0.099963protein of unknown function DUF526
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4321OMPADOMAIN922e-24 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 92.3 bits (229), Expect = 2e-24
Identities = 35/118 (29%), Positives = 54/118 (45%), Gaps = 12/118 (10%)

Query: 77 NVLFPNNSAYIAPEYYPQIEEVAIFLRQY--PTTKVTIEGHTSRTGTDETNKELSQERAN 134
+VLF N A + PE ++++ L V + G+T R G+D N+ LS+ RA
Sbjct: 220 DVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQ 279

Query: 135 AVTAVLAERFGIDRSRLTAIGYGSSRPIVLEQTPEAEIR---------NRRVVAEVTG 183
+V L + GI +++A G G S P+ + R +RRV EV G
Sbjct: 280 SVVDYLISK-GIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKG 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4326HTHTETR585e-13 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.5 bits (141), Expect = 5e-13
Identities = 34/141 (24%), Positives = 57/141 (40%), Gaps = 6/141 (4%)

Query: 2 DSKRDLILRSAEKIIATEGLHNLSMQKLAVDAGVAAGTIYRYFKDKEDLIIKLRKDVLQQ 61
R IL A ++ + +G+ + S+ ++A AGV G IY +FKDK DL ++ +
Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69

Query: 62 IASKLLENIDE--GSFDEKFRRLWFNIVELGREQSHANLSFAQYSHL---PGVDAPEHQA 116
I LE + G R + +++E + L H G A QA
Sbjct: 70 IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQA 129

Query: 117 FEREIFQPLHQLFEQAKGQGV 137
+R + + EQ +
Sbjct: 130 -QRNLCLESYDRIEQTLKHCI 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4327RTXTOXIND445e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.4 bits (105), Expect = 5e-07
Identities = 23/130 (17%), Positives = 33/130 (25%), Gaps = 35/130 (26%)

Query: 71 TISNELAGRVTSINFENGSRVEKGQLLAELDAKVERANLKSKMVQLPAAEADFKRLSKLY 130
I V I + G V KG +L +L A A+ L A + R L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 131 -----------------------------------AQKSVSKQDLDNSESKYLALQADIE 155
Q S + E +A+
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 156 SLKATIERRE 165
++ A I R E
Sbjct: 218 TVLARINRYE 227



Score = 43.7 bits (103), Expect = 8e-07
Identities = 37/253 (14%), Positives = 75/253 (29%), Gaps = 71/253 (28%)

Query: 88 GSRVEKGQLLAELDAKVERANLKSKMVQLPAAEADFKRLSKLYAQKSVSKQDLDNSESKY 147
+ + AE + R N ++ S L +++++K + E+KY
Sbjct: 204 QKELNLDKKRAERLTVLARINRYEN--LSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKY 261

Query: 148 LALQADIESLKATIER-------------------------------------------- 163
+ ++ K+ +E+
Sbjct: 262 VEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKN 321

Query: 164 ------REISAPFSGLVGIRNIN-LGEYLQPGT---DIVRLEDISTMKIRFTIPQTQLPR 213
I AP S V ++ G + IV +D T+++ + +
Sbjct: 322 EERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDD--TLEVTALVQNKDIGF 379

Query: 214 IAVGQKIHVFVDSYPEQ---PFEGEIAAIEP--------AVFYQSGLIQVQARIP--NDN 260
I VGQ + V+++P G++ I + + + + + N N
Sbjct: 380 INVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLGLVFNVIISIEENCLSTGNKN 439

Query: 261 AKLRSGMFARVSI 273
L SGM I
Sbjct: 440 IPLSSGMAVTAEI 452


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4328ACRIFLAVINRP7890.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 789 bits (2038), Expect = 0.0
Identities = 318/1037 (30%), Positives = 539/1037 (51%), Gaps = 34/1037 (3%)

Query: 5 DIFIRRPVLAASISFLLLLLGFNALNSMQVREYPKMTNTVVTVSTSYYGADANLIQGFIT 64
+ FIRRP+ A ++ +L++ G A+ + V +YP + V+VS +Y GADA +Q +T
Sbjct: 3 NFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVT 62

Query: 65 QPLEQALAQADNVDFMTSESF-LGTSKISVYMKLNTDPNGALADILAKVNSVRSQLPKEA 123
Q +EQ + DN+ +M+S S G+ I++ + TDP+ A + K+ LP+E
Sbjct: 63 QVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEV 122

Query: 124 EDPSVEMSTGSQTSVLYISFFSDQINSSQ--LTDYLERVVKPQLFTIDGVAKVNLYGGIK 181
+ + + S + ++ F SD ++Q ++DY+ VK L ++GV V L+G +
Sbjct: 123 QQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA-Q 181

Query: 182 YAMRIWLDPARMGAFNLSASDVMQVLQANNYQSAVGQTNSVYTL------FNGTADTQVA 235
YAMRIWLD + + L+ DV+ L+ N Q A GQ L + A T+
Sbjct: 182 YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 236 TIEELKRLVI-GSKDGLVVRLGDIADVSLEKSHDIYRALANGKEAVVIGLDVTPTANPLT 294
EE ++ + + DG VVRL D+A V L + A NGK A +G+ + AN L
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 295 VAADTRALLPEIERNLPPSIESSILYDSSLAIDESIKEVVKTIGEAAIIVIVVITLFLGS 354
A +A L E++ P ++ YD++ + SI EVVKT+ EA ++V +V+ LFL +
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 355 LRAVVIPIVTIPLSLIGVAIIMQMFGFTLNLMTLLAMVLAIGLVVDDAIVVVENVDRHIK 414
+RA +IP + +P+ L+G I+ FG+++N +T+ MVLAIGL+VDDAIVVVENV+R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 LGESPFRAAII-GTREIAVPVISMTITLAAVYAPIALMGGITGSLFKEFALTLAGSVFIS 473
+ P + A +I ++ + + L+AV+ P+A GG TG+++++F++T+ ++ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 474 GIVALTLSPMMCAKILKP-----HTTPNRFEMGVENFLTGLTRRYSNMLDAVMLHRPVIV 528
+VAL L+P +CA +LKP H F Y+N + ++ +
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 529 AFAIIVFASLPVLFKFIPSELAPNEDKGVVMMMGTAPSTANLDYIQANMGLVTDMIKAQP 588
++ A + VLF +PS P ED+GV + M P+ A + Q + VTD
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 589 ESAASLAF----VGVPSSSQAFGIA--PLVPWSERDKSQKQMQEFFAK---EVKRIPGMA 639
++ F +Q G+A L PW ER+ + + + E+ +I
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 640 ITTFQMPE--LPGASSGLPIQFVITTSNSFASLFQIGTSVLEKVQKSPLFVYSEI-NLKF 696
+ F MP G ++G + + +L Q +L + P + S N
Sbjct: 662 VIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLE 721

Query: 697 DSGTMKLHIKRDLAGTYGVTMQDIGITLATMMSDGYVNRINLDGRSYEVIPQVERKLRAN 756
D+ KL + ++ A GV++ DI T++T + YVN GR ++ Q + K R
Sbjct: 722 DTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRML 781

Query: 757 PESLANYYVKAADGKSIPLSSLVEIEMVAEPRSLPHFNQMNALTVGGVASPGVAIGDAIS 816
PE + YV++A+G+ +P S+ V L +N + ++ + G A+PG + GDA++
Sbjct: 782 PEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMA 841

Query: 817 FLQNIGDNELPKGYSYDFLGEARQFVTEGSALYATFLLAIAIIFLVLASQFESLKDPLVI 876
++N+ ++LP G YD+ G + Q G+ A ++ ++FL LA+ +ES P+ +
Sbjct: 842 LMENL-ASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 877 LVSVPLAISGALIVLGWTHVFGLAKINIYTQVGLITLVGLITKHGILMCEVAKEEQLHRG 936
++ VPL I G L+ + K ++Y VGL+T +GL K+ IL+ E AK+ G
Sbjct: 901 MLVVPLGIVGVLLAATLFNQ----KNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 937 LSKLEAIKLAATIRLRPILMTTAAMIAGLLPLLFASGAGAVARFNIGVVIVAGLSIGTIF 996
+EA +A +RLRPILMT+ A I G+LPL ++GAG+ A+ +G+ ++ G+ T+
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 997 TLFVLPVIYTYLAEKHE 1013
+F +PV + + +
Sbjct: 1017 AIFFVPVFFVVIRRCFK 1033


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4329PF06580250.049 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 24.8 bits (54), Expect = 0.049
Identities = 7/29 (24%), Positives = 14/29 (48%)

Query: 46 MVSREEFEVQQHVLLKTREKLEALQAQVN 74
+ E + + + +L AL+AQ+N
Sbjct: 143 NYKQAEIDQWKMASMAQEAQLMALKAQIN 171


82SO_4434SO_4446N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4434118-3.631750type I pilus assembly chaperone CooB
SO_4437114-2.402644ISSod3 transposase TnpA_ISSod3
SO_4439013-2.243118type I pilus outer membrane usher porin CooC
SO_4441-213-2.262554ISSod1 transposase TnpA_ISSod1
SO_4444-212-1.804801two component signal transduction system
SO_4445-210-0.795061two component signal transduction system hybrid
SO_4446-1193.415705ABC-type molybdate uptake system ATPase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4434TACYTOLYSIN310.003 Bacterial thiol-activated pore-forming cytolysin sig...
		>TACYTOLYSIN#Bacterial thiol-activated pore-forming cytolysin

signature.
Length = 574

Score = 31.1 bits (70), Expect = 0.003
Identities = 16/68 (23%), Positives = 29/68 (42%), Gaps = 3/68 (4%)

Query: 30 DYMPSNKTTYLKKIMNMGDSTAFI---KISINEIKYDENGQPYEVPEQEAENRALIASPS 86
+Y+ + T Y +N+ A++ +I +EI YD+ G+ + N SP
Sbjct: 454 EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTSPF 513

Query: 87 RLIIPAKG 94
+IP
Sbjct: 514 STVIPLGA 521


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4439PF00577541e-09 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 54.5 bits (131), Expect = 1e-09
Identities = 119/773 (15%), Positives = 229/773 (29%), Gaps = 137/773 (17%)

Query: 120 IEYNLENSQL--SILTANIEKQPSSSHYYALPEQGSYGALFRRQLNWSQSNESDYAGRY- 176
+ ++ +L +I A + + L + G L + + ++ G
Sbjct: 147 AQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSV-QNRIGGNSH 205

Query: 177 ----NLQAQASIGQWTTLLEGEVTQNNEETLNST-----IQQLYTERQNEGHFYRLGYFS 227
NLQ+ +IG W + N+ ++ + + + ER RL
Sbjct: 206 YAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGD 265

Query: 228 PYAQGLVRQPIIYSGSSQAVMGVMLGDSNQLLVD--QAYASA-TPIYVTPNRAGVVEIYQ 284
Y QG + G L + +L D + +A I + V I Q
Sbjct: 266 GYTQGDI-------FDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQ---VTIKQ 315

Query: 285 NGQLINSQQINAGLQAIDTKVLPSGIYEVEIRILE-DGQVTERRREIINKPIQWQNTEEP 343
NG I + + G I+ ++++ I E DG Q P
Sbjct: 316 NGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGST--------------QIFTVP 361

Query: 344 F------------RVNLFAGEKLQDITNWDD------ESRRGLSTGVLAN--YLLTPAII 383
+ R ++ AGE + GL G L
Sbjct: 362 YSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYR 421

Query: 384 LGAAAQFVDKGWHYSVSADWDW-----NQQLRFFGNWV------MNDQQGSDFDL----- 427
+ G ++S D + G V ++ G++ L
Sbjct: 422 AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRY 481

Query: 428 ---------QAVYSYRQGSFVLS----HQQQLSQSQSSDITNSNPKKTSASLQHNLGRGH 474
YS G + + Q + + ++ + K ++ LGR
Sbjct: 482 STSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTS 541

Query: 475 SVSAYLSHQSEQGQG-----IDLGWQYSGKLWGRQMSWSLNAFDRPGSINTSGQRDQGGA 529
++ SHQ+ G G + ++W+L+ + Q+ +
Sbjct: 542 TLYLSGSHQTYWGTSNVDEQFQAGL---NTAFED-INWTLSYS----LTKNAWQKGRDQM 593

Query: 530 LYFSMNLGDQKRRMGGGLGSRTSRSG-GQEQHAYVDYQQQLDWRGIETIGMGL------- 581
L ++N+ Q +HA Y D G T G+
Sbjct: 594 LALNVNI---------PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED 644

Query: 582 NTDSYGVGA-TANSRFSNNIVSGDAYAQSSSYNSGITGGINLDSMVALGQQGKLGISGQS 640
N SY V A N+ +G A G + +Q G+SG
Sbjct: 645 NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDD---IKQLYYGVSGGV 701

Query: 641 QNHDAGMIIDVMADTPDIILQAEDEHGGSVTLKP-------GRNLVP-VTAYRSGSVQLA 692
H G+ + + ++++A V + G ++P T YR V L
Sbjct: 702 LAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALD 761

Query: 693 IQDYDDTPSVIQPSVLHYHLNKGGVSYQQIRVMKTVTVIGRLVNQQGLPLKGALIANHAG 752
D + + +G + + + + ++ L + GA++ + +
Sbjct: 762 TNTLADNVDLDNAVA-NVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESS 820

Query: 753 RSLSEA--DGFFAVEMSERHPSLQVEY--QGVQECNLELDLHRVKREQNLLLV 801
+S +G + +QV++ + C L ++Q L +
Sbjct: 821 QSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQL 873


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4444HTHFIS562e-11 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 55.6 bits (134), Expect = 2e-11
Identities = 21/86 (24%), Positives = 46/86 (53%), Gaps = 6/86 (6%)

Query: 2 AFKIILADDHPIILTGVRSLMAEMQPSCDIVAEANNVSELWRTLEQHECDLLITDFSMPN 61
I++ADD I T + ++ I + N + LWR + + DL++TD MP+
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRITS---NAATLWRWIAAGDGDLVVTDVVMPD 59

Query: 62 DDNVDGMAMIKQLRRKYPNLPIIVLT 87
+ + ++ ++++ P+LP++V++
Sbjct: 60 E---NAFDLLPRIKKARPDLPVLVMS 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4445HTHFIS626e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.2 bits (151), Expect = 6e-12
Identities = 25/124 (20%), Positives = 49/124 (39%), Gaps = 7/124 (5%)

Query: 912 KILLIEDHQLNQILVQRQLKQLRLDCDIAEHGVQALDLMQQTHYDLILCDCRMPVMDGYT 971
IL+ +D + ++ + L + D I + + DL++ D MP + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 972 FTQQVRAQEIDGQHVPIIALTANVLNEQKQRCAAVEMD--DLLAKPLHLNELHEMLLKWL 1029
+++ +P++ ++A N A E D L KP L EL ++ + L
Sbjct: 65 LLPRIKKA---RPDLPVLVMSAQ--NTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 1030 PHSK 1033
K
Sbjct: 120 AEPK 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4446PF05272320.003 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 32.4 bits (73), Expect = 0.003
Identities = 11/39 (28%), Positives = 17/39 (43%)

Query: 26 CKAGEVLAVVGPSGGGKTTLLRMIAGLNHPDAGSIVFGE 64
CK + + G G GK+TL+ + GL+ G
Sbjct: 593 CKFDYSVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGT 631


83SO_4468SO_4479N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_4468-1140.890267transcriptional repressor of N-ethylmaleimide
SO_4469-2121.392997NADP-dependent alcohol dehydrogenase
SO_4470-1111.014220predicted periplasmic protein
SO_4471-2111.201404nitrogen-responsive two component signal
SO_4472-1110.997182nitrogen-responsive two component signal
SO_4473-2141.206765autotransporter
SO_44750132.386951cadmium and zinc efflux pump FieF
SO_44760132.216879periplasmic stress adaptor protein CpxP
SO_44770142.216185periplasmic stress-responsive two component
SO_4478-1162.605070periplasmic stress-responsive two component
SO_4479-1182.977189Sigma54 dependent transcriptional regulator with
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4468HTHTETR619e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 60.8 bits (147), Expect = 9e-14
Identities = 24/67 (35%), Positives = 32/67 (47%)

Query: 1 MKTETQSTRQHILDIGYSLIIKQGFSCLGLAQLLKAAEVPKGSFYHYFKSKEQFGEALLT 60
K E Q TRQHILD+ L +QG S L ++ KAA V +G+ Y +FK K +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64

Query: 61 GYFEQYQ 67

Sbjct: 65 LSESNIG 71


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4471PF06580392e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 38.7 bits (90), Expect = 2e-05
Identities = 35/187 (18%), Positives = 71/187 (37%), Gaps = 33/187 (17%)

Query: 167 LIIEQADRLRSLVDRL-------LGPQRPTQHSLHNIHQVVQKVYKLVEIALPANIQLKR 219
LI+E + R ++ L L Q SL + VV +L I +Q +
Sbjct: 185 LILEDPTKAREMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFEN 244

Query: 220 DYDPSIPDIEMDPDQMQQAVLNILQNAVQALEHTDGEILIRTRTQHQVTIGSQRHKLVLT 279
+P+I D+++ P +Q V N +++ + L G+IL++ + +T
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQ-GGKILLKGTKDNGT----------VT 293

Query: 280 LSIIDNGPGIPPELMDTLFYPMVTSREQGSGLGLSIAHNIARLHSG---RIDCVSSPGHT 336
L + + G + + ++ +G GL ++ G +I G
Sbjct: 294 LEVENTGSL------------ALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKV 341

Query: 337 EFIISLP 343
++ +P
Sbjct: 342 NAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4472HTHFIS5590.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 559 bits (1441), Expect = 0.0
Identities = 198/474 (41%), Positives = 294/474 (62%), Gaps = 12/474 (2%)

Query: 7 VWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTDGLTL 66
+ + DDD++IR VL +AL A + A +LW+ + ++V+D+ MP + L
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 67 LERLQIHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQ 126
L R++ P +PV++M+A + +A+ A + GA++YLPKPFD+ E I ++ RAL +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 127 SPTPVAQETQVKTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAGALHKH 186
++ ++G + AMQE++R + RL ++ ++++I G+SGTGKELVA ALH +
Sbjct: 126 PSKL--EDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY 183

Query: 187 SPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDEIGDM 246
R++ PF+A+NMAAIP+DLIESELFGHEKGAFTGA GRFEQA GGTLFLDEIGDM
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 247 PLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHRLNVI 306
P+D QTRLLRVL G++ VGG + ++ DVRI+AAT++DL+Q + +G FREDL++RLNV+
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 307 RIHLPPLSQRREDIPQLASHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQLENTC 366
+ LPPL R EDIP L HF+ A KE G++ K +E + PWPGNVR+LEN
Sbjct: 304 PLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 367 RWLTVMASGQEILPQDLPPELLKEPTSINPMAKGSQDWQSALTEWIDQKLSE-------- 418
R LT + I + + EL E ++ ++++ +++ + +
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 419 -GNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD 471
S L V E L+ AL T+G++ +AA LG RNTL +K++EL +
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4473OMPADOMAIN679e-16 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 67.3 bits (164), Expect = 9e-16
Identities = 46/202 (22%), Positives = 71/202 (35%), Gaps = 25/202 (12%)

Query: 1 MKKLSLVAGSLLSILVAGQALAATDTTGFYVGGAL-------NRVTVDVLDDAETGTGFG 53
MKK +A ++ A A AA +Y G L + E G G
Sbjct: 1 MKKT-AIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTHENQLGAG 59

Query: 54 VYGGYNFNEWFGLEANLFATGDL----GDKDVDISAGALTFTPKFTLQINDMFSAYAKVG 109
+GGY N + G E G + ++ A + T K I D Y ++G
Sbjct: 60 AFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLG 119

Query: 110 VASMAMNVDGDGFDEDF-TGFGWTYGVGVNAAVTERLNVRLSYDVTS--GDLDADHHYVN 166
+ + + ++ TG + GV A+T + RL Y T+ GD
Sbjct: 120 GMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTI----- 174

Query: 167 YVNVKDIDTDIKQLAIGVHYQF 188
D L++GV Y+F
Sbjct: 175 -----GTRPDNGMLSLGVSYRF 191


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4477HTHFIS951e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 94.9 bits (236), Expect = 1e-24
Identities = 45/163 (27%), Positives = 76/163 (46%), Gaps = 3/163 (1%)

Query: 2 SRILLIDDDLGLSELLGQLLELEGFKLTLAYDGKQGLELALATDYDLILLDVMLPKLNGF 61
+ IL+ DDD + +L Q L G+ + + + A D DL++ DV++P N F
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 EVLRALRQH-KQTPVLMLTARGDEIDRVVGLEIGADDYLPKPFNDRELIARIRAIIRRSH 120
++L +++ PVL+++A+ + + E GA DYLPKPF+ ELI I +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 121 LTAQEIHATPAQEFGDLRLDPSRQEAYCNEQLIILTGTEFTLL 163
++ + + QE Y L L T+ TL+
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIY--RVLARLMQTDLTLM 164


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4478PF06580394e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 38.7 bits (90), Expect = 4e-05
Identities = 25/126 (19%), Positives = 51/126 (40%), Gaps = 6/126 (4%)

Query: 274 IAYEAEQLEQLIAELLELSRVKLSTNETKVCLGLAESLSQVLDDAEFEADQQGKKIT--I 331
I + + +++ L EL R L + + LA+ L+ V + + Q ++
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYSNARQVS-LADELTVVDSYLQLASIQFEDRLQFEN 244

Query: 332 DIDEAIELSHYPK-SLSRAIENLLRNAIRYAKSD--IHLHASQTSGQVQITIKDDGPGID 388
I+ AI P + +EN +++ I I L ++ +G V + +++ G
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLAL 304

Query: 389 PAELES 394
ES
Sbjct: 305 KNTKES 310


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4479HTHFIS336e-111 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 336 bits (863), Expect = e-111
Identities = 119/375 (31%), Positives = 197/375 (52%), Gaps = 20/375 (5%)

Query: 259 FHRDLALHLHTQALGVTQTKSAKTIQDKPQSQLGVRFRDPLLERAWQQANKVITKQIPLL 318
F + + +AL + ++D Q + + R ++ ++ +++ + L+
Sbjct: 106 FDLTELIGIIGRALA-EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLM 164

Query: 319 VLGETGVGKEQFVKKLHAQSSRRAQPLVAVNCAALPAELVESELFGYQAGAFTGANRSGF 378
+ GE+G GKE + LH RR P VA+N AA+P +L+ESELFG++ GAFTGA
Sbjct: 165 ITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRS- 223

Query: 379 IGKIRQAHGGFLFLDEIGEMPQAAQSRLLRVLQEREVVPVGSNQSVKVDIQIIAATHMDL 438
G+ QA GG LFLDEIG+MP AQ+RLLRVLQ+ E VG ++ D++I+AAT+ DL
Sbjct: 224 TGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDL 283

Query: 439 ESLVAQGLFRQDLFYRLNGLQVRLPALRERQ-DIERIIH---KLHRRHRSGPQTLCTELL 494
+ + QGLFR+DL+YRLN + +RLP LR+R DI ++ + + + E L
Sbjct: 284 KQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEAL 343

Query: 495 GQLMRYDWPGNLRELDNLMQVACLMAEDDEVLARAHLPEHLAKKLVNKPLAVYERQHVEN 554
+ + WPGN+REL+NL++ + D V+ R + L ++ + P+ +
Sbjct: 344 ELMKAHPWPGNVRELENLVRRLTALYPQD-VITREIIENELRSEIPDSPIEKAAARSGSL 402

Query: 555 PQNPRDKDDIGRHSADSLHGAINQNVL-------------QAYRACEGNVSQCAKRLGIS 601
+ ++++ ++ A + A A GN + A LG++
Sbjct: 403 SISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLN 462

Query: 602 RNALYRKLKQLGIKD 616
RN L +K+++LG+
Sbjct: 463 RNTLRKKIRELGVSV 477


84SO_4686SO_4694N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
SO_46860131.592695UDP-glucuronate 4-epimerase WcvA
SO_4687-1141.621788UDP-glucose dehydrogenase WcvB
SO_46880141.876215glycosyl transferase family 2
SO_4689-1161.611297membrane protein COG0398 family
SO_4690-1151.374488glycosyl transferase family 83
SO_4692-2170.831413proton-coupled multidrug efflux pump permease
SO_46930140.550546proton-coupled multidrug efflux pump MFP
SO_46941140.186226TMAO reductase system outer membrane porin TorF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4686NUCEPIMERASE5650.0 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 565 bits (1457), Expect = 0.0
Identities = 233/333 (69%), Positives = 268/333 (80%), Gaps = 1/333 (0%)

Query: 1 MKYLVTGAAGFIGANVSKRLCAMGHEVVGIDNLNDYYDVALKLARLAPLEALSNFHFIKL 60
MKYLVTGAAGFIG +VSKRL GH+VVGIDNLNDYYDV+LK ARL L F F K+
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQ-PGFQFHKI 59

Query: 61 DLADREGIAKLFAQQGFQRVIHLAAQAGVRYSLDNPLAYADSNLVGHLTILEGCRHHKIE 120
DLADREG+ LFA F+RV + VRYSL+NP AYADSNL G L ILEGCRH+KI+
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119

Query: 121 HLVYASSSSVYGLNQKMPFSTEDSVDHPISLYAATKKANELMSHTYSHLYQLPTTGLRFF 180
HL+YASSSSVYGLN+KMPFST+DSVDHP+SLYAATKKANELM+HTYSHLY LP TGLRFF
Sbjct: 120 HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFF 179

Query: 181 TVYGPWGRPDMALFKFTKAILAGETIDVYNHGDLSRDFTYIDDIVEGIIRVQDKPPSPTP 240
TVYGPWGRPDMALFKFTKA+L G++IDVYN+G + RDFTYIDDI E IIR+QD P
Sbjct: 180 TVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADT 239

Query: 241 DWRVETGTPANSSAPYRVFNIGNGSPVQLLDFITALERALGIEAKKQFLPMQPGDVHATW 300
W VETGTPA S APYRV+NIGN SPV+L+D+I ALE ALGIEAKK LP+QPGDV T
Sbjct: 240 QWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGDVLETS 299

Query: 301 ADTEDLFKAVGYKSQVDIDTGVAKFVDWYRNFY 333
ADT+ L++ +G+ + + GV FV+WYR+FY
Sbjct: 300 ADTKALYEVIGFTPETTVKDGVKNFVNWYRDFY 332


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4692ACRIFLAVINRP12550.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1255 bits (3250), Expect = 0.0
Identities = 657/1032 (63%), Positives = 808/1032 (78%), Gaps = 4/1032 (0%)

Query: 1 MARFFIDRPIFAWVIALIIMLAGVLSIRTLPVSQYPSIAPPTVVISANYPGASAKIVEDS 60
MA FFI RPIFAWV+A+I+M+AG L+I LPV+QYP+IAPP V +SANYPGA A+ V+D+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQRMKGIDHLRYIASTSDSFGNAEITLTFNAEADPDIAQVQVQNKLQGAMTLLPQ 120
VTQVIEQ M GID+L Y++STSDS G+ ITLTF + DPDIAQVQVQNKLQ A LLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQAQGVDVNKSSSGFLMVLGFVSTDGSLDKGDIADYVGANVQDPMSRVPGVGEIQLFGA 180
EVQ QG+ V KSSS +LMV GFVS + + DI+DYV +NV+D +SR+ GVG++QLFGA
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWLDPLKLTQYNLTSLEVISAIRAQNAQVSAGQLGGTPSIQGQELNATVSAQSRL 240
QYAMRIWLD L +Y LT ++VI+ ++ QN Q++AGQLGGTP++ GQ+LNA++ AQ+R
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 QTPEEFRKIILKSDTSGANVFLGDVARVELGSESYAVVSFYNGKPATGLAIKLATGANAL 300
+ PEEF K+ L+ ++ G+ V L DVARVELG E+Y V++ NGKPA GL IKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTAEAVRDKVEELRPFFPQGLDVVYPYDTTPFVEKSIEGVVHTLLEAIVLVFVIMYLFLQ 360
DTA+A++ K+ EL+PFFPQG+ V+YPYDTTPFV+ SI VV TL EAI+LVF++MYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLLGTFAILSATGFSINTLTMFAMVLAIGLLVDDAIVVVENVERVM 420
N RATLIPTIAVPVVLLGTFAIL+A G+SINTLTMF MVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 SEEGLSPLEATRKSMDQITGALVGIGLTLSAVFVPMAFMSGSTGVIYRQFSITIVSAMAL 480
E+ L P EAT KSM QI GALVGI + LSAVF+PMAF GSTG IYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATMLKPVQKGHGHIETGFFGWFNRNFDRLTNRYESSVAGIVKRGFRV 540
SVLVALILTPALCAT+LKPV H + GFFGWFN FD N Y +SV I+ R
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 541 MMIYVALVVAVGWIFMRMPTAFLPDEDQGILFTQAILPTNSTQESTLKVLDKVSDHFMA- 599
++IY +V + +F+R+P++FLP+EDQG+ T LP +TQE T KVLD+V+D+++
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 600 -EEGVRSVFSVAGFSFAGQGQNMGIAFVGLKDWSEREAPGMDVQSIAGRAMGAFSQIKDA 658
+ V SVF+V GFSF+GQ QN G+AFV LK W ER +++ RA +I+D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 659 FVFAFVPPAVIELGTANGFDMYLQDKNGQGHDKLIAARNQLLGMAAQNP-NLMGVRPNGQ 717
FV F PA++ELGTA GFD L D+ G GHD L ARNQLLGMAAQ+P +L+ VRPNG
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 718 EDAPIYQLHIDHAKLSALGVDIANVNSVLATAWGGSYVNDFIDRGRVKKVFVQGDAQYRM 777
ED ++L +D K ALGV ++++N ++TA GG+YVNDFIDRGRVKK++VQ DA++RM
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 778 QPEDLNTWYVRNNKGDMVPFSAFATGSWEYGSPRLERFNGLPAVNIQGATAPGFSTGAAM 837
PED++ YVR+ G+MVPFSAF T W YGSPRLER+NGLP++ IQG APG S+G AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 838 TIMEDLVKQLPPGFGIEWNGLSYEERLSGNQAPALYALSILVVFLVLAALYESWSVPFAV 897
+ME+L +LP G G +W G+SY+ERLSGNQAPAL A+S +VVFL LAALYESWS+P +V
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 898 ILVVPLGIIGALLAMNGRGLPNDVFFQVGLLTTVGLATKNAILIVEFAKEFYEK-GAGLV 956
+LVVPLGI+G LLA NDV+F VGLLTT+GL+ KNAILIVEFAK+ EK G G+V
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 957 EATLHAVRVRLRPILMTSLAFGLGVVPLAISTGVGSGSQNAIGTGVLGGMMSSTFLGIFF 1016
EATL AVR+RLRPILMTSLAF LGV+PLAIS G GSG+QNA+G GV+GGM+S+T L IFF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1017 VPLFFVIVERIF 1028
VP+FFV++ R F
Sbjct: 1021 VPVFFVVIRRCF 1032



Score = 85.3 bits (211), Expect = 8e-19
Identities = 76/527 (14%), Positives = 164/527 (31%), Gaps = 44/527 (8%)

Query: 534 VKRGFRVMMIYVALVVAVGWIFMRMPTAFLPDEDQGILFTQAILPTNSTQESTLKVLDKV 593
++R ++ + L++A +++P A P + A P Q V +
Sbjct: 6 IRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQVI 65

Query: 594 SDHFMAEEGVRSVFSVAGFSFAGQGQNMGIAFVGLKDWSEREAPGMDVQSIAGRAMGAFS 653
+ + + + S S + + + F G D +
Sbjct: 66 EQNMNGIDNLMYMSST---SDSAGSVTITLTF----------QSGTDPDIAQVQVQNKLQ 112

Query: 654 QIKDAFVFAFVPPAVIELGTANGFDMYL----QDKNGQGHDKLIAARNQLLGMAAQNPNL 709
+ +++ + M + D + + ++ +
Sbjct: 113 LATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGV 172

Query: 710 MGVRPNGQEDAPIYQLHI--DHAKLSALGVDIANVNSVLATA----WGGSYVNDFIDRGR 763
V+ G + Y + I D L+ + +V + L G G+
Sbjct: 173 GDVQLFGAQ----YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQ 228

Query: 764 VKKVFVQGDAQYRMQPEDLNTWYVRNNK-GDMVPFSAFAT---GSWEYGSPRLERFNGLP 819
+ +++ PE+ +R N G +V A G Y + R NG P
Sbjct: 229 QLNASIIAQTRFK-NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNV--IARINGKP 285

Query: 820 AVNIQGATAPGFST----GAAMTIMEDLVKQLPPGFGIEW---NGLSYEERLSGNQAPAL 872
A + A G + A + +L P G + + + +
Sbjct: 286 AAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLF 345

Query: 873 YALSILVVFLVLAALYESWSVPFAVILVVPLGIIGALLAMNGRGLPNDVFFQVGLLTTVG 932
A I++VFLV+ ++ + VP+ ++G + G + G++ +G
Sbjct: 346 EA--IMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIG 403

Query: 933 LATKNAILIVE-FAKEFYEKGAGLVEATLHAVRVRLRPILMTSLAFGLGVVPLAISTGVG 991
L +AI++VE + E EAT ++ ++ ++ +P+A G
Sbjct: 404 LLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGST 463

Query: 992 SGSQNAIGTGVLGGMMSSTFLGIFFVPLFFVIVERIFSKRERKAKEK 1038
++ M S + + P + + S + K
Sbjct: 464 GAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGG 510


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4693RTXTOXIND409e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 40.2 bits (94), Expect = 9e-06
Identities = 38/211 (18%), Positives = 80/211 (37%), Gaps = 32/211 (15%)

Query: 99 TYKAALVSANADLARANASLASAKAKAARYQQLVKTNAISKQDFD-EADAAYKEALASVT 157
+ V A +L + L +++ ++ + Q F E ++ ++
Sbjct: 256 EQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLV---TQLFKNEILDKLRQTTDNIG 312

Query: 158 VAEAAINTAKINLEYTEVLAPISGRIGKSSV-TAGALVTANQSQTLATIQQLDPINVDIA 216
+ + + + + + AP+S ++ + V T G +VT ++TL + I
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTT--AETL----------MVIV 360

Query: 217 QSSAQLLRLKAKLKQ---GKLLAADNADVQLVLVDGTVYGH-TGKLQ--FAEVSVDQNTG 270
L + A ++ G + NA +++ T YG+ GK++ + DQ G
Sbjct: 361 PEDDTLE-VTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLG 419

Query: 271 SV--ILRA------EFPNPDGVLLPGMYVRA 293
V ++ + N + L GM V A
Sbjct: 420 LVFNVIISIEENCLSTGNKNIPLSSGMAVTA 450



Score = 37.9 bits (88), Expect = 5e-05
Identities = 36/174 (20%), Positives = 66/174 (37%), Gaps = 45/174 (25%)

Query: 57 GRSKAFLEAEVRPQVNGIITKRSFV-EGGNVKQGESLYQIDSATYKAALVSANADLARAN 115
GRSK E++P N I+ K V EG +V++G+ L ++ + + A AD +
Sbjct: 94 GRSK-----EIKPIENSIV-KEIIVKEGESVRKGDVLLKLTA-------LGAEADTLKTQ 140

Query: 116 ASLASAKAKAARYQQLVKTNAISKQDFDEADAAYKEALASVTVAEAAINTAKINLEYTEV 175
+SL A+ + RYQ L + +I E + +V+ E T+ I
Sbjct: 141 SSLLQARLEQTRYQILSR--SIELNKLPELKLPDEPYFQNVSEEEVLRLTSLI------- 191

Query: 176 LAPISGRIGKSSVTAGALVTANQSQTLATIQQLDPINVDIAQSSAQLLRLKAKL 229
+ Q Q +++ + A+ L + A++
Sbjct: 192 ----------------------KEQFSTWQNQKYQKELNLDKKRAERLTVLARI 223


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
SO_4694ECOLNEIPORIN280.038 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 27.8 bits (62), Expect = 0.038
Identities = 20/93 (21%), Positives = 32/93 (34%), Gaps = 7/93 (7%)

Query: 97 FIGKAGEFG--DSGFTYDVMLFSYLYPGASYSNYTELWLKVGKQFGRANLQLEITPTVDD 154
FIG G FG G V+ + + +L V K + + +
Sbjct: 97 FIGLKGGFGKLRVGRLNSVL---KDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPE 153

Query: 155 WFGVDGWHGVNYALHPSYSFDNGVKISASVGYQ 187
+ G+ G V YAL+ + N A Y+
Sbjct: 154 FAGLSG--SVQYALNDNAGRHNSESYHAGFNYK 184



 
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