PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
GenomeY394.gbThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in CP020753 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1B7485_00275B7485_00315Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_00275-2203.057797molecular chaperone DjlA
B7485_00280-2193.755507bifunctional tRNA pseudouridine(32)
B7485_00285-2193.598711RNA polymerase-associated protein RapA
B7485_00290-2163.455206DNA polymerase II
B7485_00295-1163.366356L-ribulose-5-phosphate 4-epimerase
B7485_00300-1163.581351L-arabinose isomerase
B7485_003051174.456361ribulokinase
B7485_003100173.235992DNA-binding transcriptional regulator AraC
B7485_003150183.613964DedA family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_0027556KDTSANTIGN290.023 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 28.8 bits (64), Expect = 0.023
Identities = 32/120 (26%), Positives = 51/120 (42%), Gaps = 18/120 (15%)

Query: 157 IAEELGISRAQFD-----QFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLG 211
EEL R FD F+ + QQQ G G QQAQ T ++A
Sbjct: 310 TLEEL---RDSFDGYINNAFVNQIHLNFVMPPQAQQQQGQGQQQQAQ--ATAQEAVAAAA 364

Query: 212 VKPTDDATTIKRAYRKLMS-EHHPDKLVAKGLPPEMMEMAKQKAQEIQ-QAYELIKQQKG 269
V+ + + I + Y+ L+ + H G+ M ++A Q+ ++ + Q KQQ+G
Sbjct: 365 VRLLNGSDQIAQLYKDLVKLQRH------AGIRKAMEKLAAQQEEDAKNQGKGDCKQQQG 418


2B7485_00560B7485_00595Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_005602291.411554regulatory signaling modulator protein AmpE
B7485_005653371.444042aromatic amino acid transporter AroP
B7485_005705381.979532pyruvate dehydrogenase complex repressor
B7485_005755411.451984hypothetical protein
B7485_005803371.851120pyruvate dehydrogenase (acetyl-transferring),
B7485_005852292.018779pyruvate dehydrogenase complex
B7485_005901272.660532dihydrolipoyl dehydrogenase
B7485_005950203.007236hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_00585RTXTOXIND320.007 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.1 bits (73), Expect = 0.007
Identities = 15/60 (25%), Positives = 29/60 (48%), Gaps = 2/60 (3%)

Query: 119 EVTEILVKVGDKV-EAEQSLITVEGDKASMEVPAPFAGTVKEIKVN-VGDKVSTGSLIMV 176
E+ + L + D + L E + + + AP + V+++KV+ G V+T +MV
Sbjct: 299 EILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMV 358



Score = 32.1 bits (73), Expect = 0.008
Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)

Query: 26 DKVEAEQSLITVEGDKASMEVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAP 85
+ V +T G S E+ + IVKEI V G+ + G +++ + AD
Sbjct: 81 EIVATANGKLTHSGR--SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLK 138

Query: 86 AQA 88
Q+
Sbjct: 139 TQS 141



Score = 31.0 bits (70), Expect = 0.015
Identities = 17/106 (16%), Positives = 38/106 (35%), Gaps = 4/106 (3%)

Query: 230 DKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIMIFEVEGAAPAAAP 289
+ VA +T G S E+ +VKE+ V G+ V+ G + + ++ A
Sbjct: 81 EIVATANGKLTHSGR--SKEIKPIENSIVKEIIVKEGESVRKGDV--LLKLTALGAEADT 136

Query: 290 AKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLARE 335
K +++ + + + + P + ++ E
Sbjct: 137 LKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEE 182


3B7485_00720B7485_00775Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_007200143.085542tRNA glutamyl-Q(34) synthetase GluQRS
B7485_007251162.918368RNA polymerase-binding transcription factor
B7485_00730-1153.167900DNA/RNA nuclease SfsA
B7485_00740-1132.928367RNA 2',3'-cyclic phosphodiesterase
B7485_00745-2142.807310ATP-dependent helicase HrpB
B7485_00750-2173.224206hypothetical protein
B7485_00755-2173.358192penicillin-binding protein 1B
B7485_00760-1153.194730ferrichrome porin FhuA
B7485_007650174.256499iron-hydroxamate transporter ATP-binding
B7485_007701153.974470iron-hydroxamate transporter substrate-binding
B7485_007750143.822628Fe3+-hydroxamate ABC transporter permease FhuB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_00770FERRIBNDNGPP5070.0 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 507 bits (1308), Expect = 0.0
Identities = 292/296 (98%), Positives = 293/296 (98%)

Query: 1 MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVA 60
MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVA
Sbjct: 1 MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWLPVELLLALGIVPYGVA 60

Query: 61 DTINYRLWVSEPPLPDSVIDVGLRTEPNLELMTEMKPSFMVWSAGYGPSSEMLARIAPGR 120
DTINYRLWVSEPPLPDSVIDVGLRTEPNLEL+TEMKPSFMVWSAGYGPS EMLARIAPGR
Sbjct: 61 DTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGR 120

Query: 121 GFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLT 180
GFNFSDGK PLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLT
Sbjct: 121 GFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLT 180

Query: 181 TLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDH 240
TLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDH
Sbjct: 181 TLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDH 240

Query: 241 DNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYGATLSAMHFVRVLDNAIGGKA 296
DNSKDMDALMATPLWQAMPFVR GRFQRVPAVWFYGATLSAMHFVRVLDNAIGGKA
Sbjct: 241 DNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVLDNAIGGKA 296


4B7485_01090B7485_01205Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_01090-1154.638307SAM-dependent methyltransferase
B7485_01095-1185.452267ribonuclease HI
B7485_011000216.205836DNA polymerase III subunit epsilon
B7485_011101205.515387*cytoplasmic protein
B7485_011151194.301454type VI secretion system ImpG/VasA family
B7485_011202182.605545cytoplasmic protein
B7485_011254230.375899impE family protein
B7485_01130323-0.170135hypothetical protein
B7485_01135326-1.099114lysis protein
B7485_01140227-0.398565IS3 family transposase
B7485_01145326-0.947987terminase
B7485_01150326-0.426897hypothetical protein
B7485_01160428-0.114144portal protein
B7485_01165529-0.329982scaffolding protein
B7485_01170528-0.522533coat protein
B7485_01175628-0.706610hypothetical protein
B7485_01185628-1.294998recombinase RmuC
B7485_01195429-1.152430hypothetical protein
B7485_01200429-0.966716hypothetical protein
B7485_01205327-0.261219hypothetical protein
5B7485_01285B7485_01445Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_01285216-1.777896transcriptional regulator
B7485_01290625-4.146988outer membrane autotransporter barrel
B7485_01300523-1.201864transposase
B7485_01305524-1.967915hypothetical protein
B7485_01310424-3.551496delta-aminolevulinic acid dehydratase
B7485_01315320-2.185591taurine dioxygenase
B7485_01320014-0.448089taurine transporter subunit
B7485_013250130.329210taurine import ATP-binding protein TauB
B7485_013301160.957213taurine ABC transporter substrate-binding
B7485_013350172.507310regulator
B7485_013400193.689500hypothetical protein
B7485_01345-1213.682613universal stress protein
B7485_013500180.880490hypothetical protein
B7485_01355118-1.061988lactate utilization protein C
B7485_01360117-3.075911iron-sulfur cluster-binding protein
B7485_01365323-4.853627hypothetical protein
B7485_01375221-4.195564hypothetical protein
B7485_01380122-2.858941hypothetical protein
B7485_01390-122-3.032276hypothetical protein
B7485_01400217-2.704882type VI secretion protein Vgr
B7485_01410218-1.987578hypothetical protein
B7485_014203223.293785type IV secretion protein Rhs
B7485_014303314.730208type IV secretion protein Rhs
B7485_014353324.703570type IV secretion protein Rhs
B7485_014402294.736984hypothetical protein
B7485_014451284.364335amidohydrolase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01295PRTACTNFAMLY702e-16 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 70.1 bits (171), Expect = 2e-16
Identities = 41/176 (23%), Positives = 73/176 (41%), Gaps = 3/176 (1%)

Query: 6 LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDAGYVTPYGSISGLFQSG 65
L S ND S+G V G + G L+ G F D ++ P ++ G
Sbjct: 736 LRASRLENDFKVAGSDGYAVKGKYRTHGVGASLEAGRRFTHADGWFLEPQAELAVFRAGG 795

Query: 66 DDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNG 125
Y+ +N ++V + S+ LG++ G + + + PY K + + + + V+
Sbjct: 796 GAYRAANGLRVRDEGGSSVLGRLGLEVGKRIELAGGRQVQPYIKASVL-QEFDGAGTVHT 854

Query: 126 DSIDNGTEGSAVRV--GLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW 179
+ I + TE R GLG + + S Y Y G + W+ + G +Y+W
Sbjct: 855 NGIAHRTELRGTRAELGLGMAAALGRGHSLYASYEYSKGPKLAMPWTFHAGYRYSW 910


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01340BINARYTOXINB300.015 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 30.0 bits (67), Expect = 0.015
Identities = 19/69 (27%), Positives = 30/69 (43%)

Query: 254 DIVRELRERTELPIGAYQVSGEYAMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYF 313
+ EL + +L + QV G A F +D E L I+ A +IF+
Sbjct: 466 NQFLELEKTKQLRLDTDQVYGNIATYNFENGRVRVDTGSNWSEVLPQIQETTARIIFNGK 525

Query: 314 ALDLAEKKI 322
L+L E++I
Sbjct: 526 DLNLVERRI 534


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01425CHANLCOLICIN300.027 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 30.4 bits (68), Expect = 0.027
Identities = 31/101 (30%), Positives = 42/101 (41%), Gaps = 9/101 (8%)

Query: 10 PVGNGGPVITT-----PPIAGESGGMSTGSAVTDVSGAAEEMAEQAAADLFGALPEPSGL 64
P + G VI T P +G GG G + ++ S A A+ + A L E +
Sbjct: 13 PYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAAR 72

Query: 65 VKAAVAAAQAAAAA---AGISDMAGAVQDAAASLAAGAPGA 102
KAA A AQA A A A + V +A A+ P A
Sbjct: 73 AKAA-AEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSA 112


6B7485_01585B7485_01840Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_01585-320-3.129779hypothetical protein
B7485_01595133-6.312784glycosyltransferase
B7485_01600140-10.200049GtrA family protein
B7485_01605246-12.820999transposase
B7485_01610446-14.134103hypothetical protein
B7485_01615548-14.229626hypothetical protein
B7485_01625234-8.957916excisionase
B7485_01630326-3.042361integrase
B7485_01635326-1.487726transposase
B7485_01640225-1.266138integrase
B7485_01645325-1.422740acyltransferase
B7485_01650326-2.346108phage tail protein
B7485_01655330-2.371478IS3 family transposase
B7485_01660434-4.638194diguanylate cyclase AdrA
B7485_01665334-4.544528pyrroline-5-carboxylate reductase
B7485_01675141-8.316637DUF188 domain-containing protein
B7485_01685134-5.709255shikimate kinase
B7485_01690127-4.143934hypothetical protein
B7485_01695024-3.187578hypothetical protein
B7485_01700-1180.599360protein AroM
B7485_01705-2161.386640hypothetical protein
B7485_01710-1162.063957hypothetical protein
B7485_01720017-1.013061recombination-associated protein RdgC
B7485_01725-121-2.357033fructokinase
B7485_01730-124-4.495532MFS transporter AraJ
B7485_01735-130-5.599608exonuclease subunit SbcC
B7485_01740-131-6.279818exonuclease sbcCD subunit D
B7485_01750021-1.055263DNA-binding response regulator
B7485_01755221-1.161968PAS domain-containing sensor histidine kinase
B7485_017603170.613443branched-chain amino acid transport system II
B7485_017702151.446220proline-specific permease ProY
B7485_017801121.737019alpha-glycosidase
B7485_017850141.983046tRNA preQ1(34) S-adenosylmethionine
B7485_01790-1132.019125tRNA guanosine(34) transglycosylase Tgt
B7485_018000152.038877preprotein translocase subunit YajC
B7485_018100141.019649protein translocase subunit SecD
B7485_01815-1141.176757protein translocase subunit SecF
B7485_018251220.147319HNH endonuclease
B7485_01830228-0.458011nucleoside-specific channel-forming protein Tsx
B7485_01835227-0.785027hypothetical protein
B7485_01840224-0.719343transcriptional regulator NrdR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01775ACETATEKNASE290.016 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 29.4 bits (66), Expect = 0.016
Identities = 17/69 (24%), Positives = 29/69 (42%), Gaps = 10/69 (14%)

Query: 187 FISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVVNILDP- 245
+G ++D+R L A + D A+LAL + R+ K++ +
Sbjct: 273 VYGISGISSDFRDLEDAAF---------KNGDKRAQLALNVFAYRVKKTIGSYAAAMGGV 323

Query: 246 DVIVLGGGM 254
DVIV G+
Sbjct: 324 DVIVFTAGI 332


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01780TCRTETA531e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 52.5 bits (126), Expect = 1e-09
Identities = 73/356 (20%), Positives = 126/356 (35%), Gaps = 36/356 (10%)

Query: 5 ILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGH---MISYYALVVVVGAPIIALF 61
+ ++AL G+G+ IM VL L ++ S H +++ YAL+ AP++
Sbjct: 11 LSTVALDAVGIGL----IMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 62 SSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSGFPHGAFFGVGAIVLSKIIK 121
S R+ + +LL +A + A+ + +L IGR+V+G GA + I
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIAD--IT 124

Query: 122 PGKVTAAVAGMVSGMTVANLLGIP-LGTYLSQECWRYTFLLIAVFNIAVMASVYFWVPDI 180
G A G +S ++ P LG + F A N + F +P+
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPES 184

Query: 181 RDEAKGNLREQ----------FHFLRSPAPWLI--FAATMFGNAGVFAWFSYVKPYMMFI 228
+ LR + + A + F + G W +
Sbjct: 185 HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG------E 238

Query: 229 SGFSETAMTFIMMLVGLGM---VLGNMLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCG 285
F A T + L G+ + M++G ++ R R + ++L F
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFAT 298

Query: 286 GMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAF--NLGSAVG 339
I + G+ LQ +L + E G G +A +L S VG
Sbjct: 299 RGWMAFPIMVLLASGGIG--MPALQAMLSRQV-DEERQGQLQGSLAALTSLTSIVG 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01785RTXTOXIND397e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 39.4 bits (92), Expect = 7e-05
Identities = 34/199 (17%), Positives = 71/199 (35%), Gaps = 14/199 (7%)

Query: 671 QQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALH 730
+ + Q + Q R Q L+ +E + E + +V +
Sbjct: 133 EADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIK 192

Query: 731 SQQQTLQQQDVLAAQSLQKAQAQFDTAL--------QASVFDDQQAFLAALMDEQTLTQL 782
Q T Q Q +L K +A+ T L + V + ++L+ +Q +
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIA-- 250

Query: 783 EQLKQNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQEL-AQTHQKLREN 841
K + Q + V + +Q +Q + L+ + + Q + KLR+
Sbjct: 251 ---KHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQT 307

Query: 842 TTSQGEIRQQLKQDADNRQ 860
T + G + +L ++ + +Q
Sbjct: 308 TDNIGLLTLELAKNEERQQ 326



Score = 39.4 bits (92), Expect = 7e-05
Identities = 25/204 (12%), Positives = 59/204 (28%), Gaps = 18/204 (8%)

Query: 487 EARIKTLEAQRAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEG 546
EA ++ Q + Q ++E + E + +E + L
Sbjct: 133 EADTLKTQSSLLQARLEQ---TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLT- 188

Query: 547 AALRGQLDALTKQLQRDENEAQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQD 606
+ ++ Q Q + E R + + + + DD L Q
Sbjct: 189 SLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQA 248

Query: 607 -------EHERQL-RLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLP 658
E E + +++ + Q+ +I+ +++ + Q L
Sbjct: 249 IAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLF------KNEILD 302

Query: 659 QEDEEESWLATRQQEAQSWQQRQN 682
+ + + E ++RQ
Sbjct: 303 KLRQTTDNIGLLTLELAKNEERQQ 326



Score = 32.5 bits (74), Expect = 0.009
Identities = 16/150 (10%), Positives = 42/150 (28%), Gaps = 5/150 (3%)

Query: 731 SQQQTLQQQDVLAAQSLQKAQAQFDTA----LQASVFDDQQAFLAALMDEQTLTQLEQLK 786
+ Q + A + Q + L D+ F +E+ L +K
Sbjct: 134 ADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVS-EEEVLRLTSLIK 192

Query: 787 QNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQG 846
+ + Q + A+ + L L + ++
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKH 252

Query: 847 EIRQQLKQDADNRQQQQTLMQQIAQMTQQV 876
+ +Q + + + + Q+ Q+ ++
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEI 282


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01790FRAGILYSIN310.009 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 30.8 bits (69), Expect = 0.009
Identities = 14/70 (20%), Positives = 25/70 (35%), Gaps = 4/70 (5%)

Query: 149 KQQHLLAAITDYYQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFP 208
K+ ++ I ++Y + + + I T S+ D + + I A
Sbjct: 135 KEAQMMNEIAEFYAAPFKKTRAINEKEAFECI-YDSRTR--SAGKD-IVSVKINIDKAKK 190

Query: 209 AQNFPPADYI 218
N P DYI
Sbjct: 191 ILNLPECDYI 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01795HTHFIS957e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 95.3 bits (237), Expect = 7e-25
Identities = 33/149 (22%), Positives = 63/149 (42%), Gaps = 9/149 (6%)

Query: 4 RILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQ 63
ILV +D+A IR ++ L + G+ + + + DL++ D ++P +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 FIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRI 123
+ +K+ D+PV++++A+ ++ E GA DY+ KPF EL+ I +
Sbjct: 65 LLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA-- 120

Query: 124 SPMAVEEVIKMQGLSLDPTSHRVMAGEEP 152
E + L D + G
Sbjct: 121 -----EPKRRPSKLEDDSQDGMPLVGRSA 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01800PF06580340.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 34.1 bits (78), Expect = 0.001
Identities = 19/105 (18%), Positives = 33/105 (31%), Gaps = 26/105 (24%)

Query: 325 LVYNAVNH----TPEGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKAR 380
LV N + H P+G I ++ + VE+ G
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKN---------------- 306

Query: 381 SRQTGGSGLGLAIVKHAVNH---HESRLNIESTVGKGTRFSFVIP 422
+G GL V+ + E+++ + GK +IP
Sbjct: 307 --TKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM-VLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01840SECFTRNLCASE691e-14 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 68.7 bits (168), Expect = 1e-14
Identities = 38/183 (20%), Positives = 88/183 (48%), Gaps = 5/183 (2%)

Query: 433 IQIVEERTIGPTLGMQNIEQGLEACLAGLLVSILFMII-FYKKFGLIATSALIANLILIV 491
++I ++GP + + + + + LA +V + ++ + F +F L A AL+ +++L V
Sbjct: 135 LKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLTV 194

Query: 492 GIMSLLPGATLSMPGIAGIVLTLAVAVDANVLINERIKEEL--SNGRTVQQAIDEGYRGA 549
G+ ++L + +A ++ +++ V++ +R++E L ++ ++
Sbjct: 195 GLFAVL-QLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 550 FSSIFDANITTLIKVIILYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKR 609
S +TTL+ ++ + G I+GF GV T ++++ + IV L G R
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIV-LFIGLDR 312

Query: 610 VKK 612
K+
Sbjct: 313 NKE 315


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01845SECFTRNLCASE348e-122 Bacterial translocase SecF protein signature.
		>SECFTRNLCASE#Bacterial translocase SecF protein signature.

Length = 333

Score = 348 bits (895), Expect = e-122
Identities = 104/309 (33%), Positives = 179/309 (57%), Gaps = 12/309 (3%)

Query: 17 YDFMRWDYWAFGISGLLLIAAIVIMGVRGFNWGLDFTGGTVIEITLEEPAEIDVMRDALQ 76
+DF RW + FG + +++IA++++ V G N+G+DF GGT I ++ V R AL+
Sbjct: 14 FDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAALE 73

Query: 77 KAGFEEPMLQNFGS------SHDIMVRMPPAEGETGGQVLGSQVLKVINE------STNQ 124
+ ++ H M+R+ E G + G+Q +++N+ + +
Sbjct: 74 PLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAVDP 133

Query: 125 NAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVYVGFRFEWRLAAGVVIALTHDVIIT 184
+ E VGP V +L T +L+AA + I+ Y+ RFEW+ A G V+AL HDV++T
Sbjct: 134 ALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVLLT 193

Query: 185 LGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVSDRIRENFRKIRRGTPYEIFNVSLTQT 244
+G+ ++ ++ DLT VA+L+++ GYS+ND++VV DR+REN K + ++ N+S+ +T
Sbjct: 194 VGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLRDVMNLSVNET 253

Query: 245 LHRTLITSGTTLMVILMLYLFGGPVLEGFSLTMLIGVSIGTASSIYVASALALKLGMKRE 304
L RT++T TTL+ ++ + ++GG V+ GF M+ GV GT SS+YVA + L +G+ R
Sbjct: 254 LSRTVMTGMTTLLALVPMLIWGGDVIRGFVFAMVWGVFTGTYSSVYVAKNIVLFIGLDRN 313

Query: 305 HMLQQKVEK 313
+ +K
Sbjct: 314 KEKKDPSDK 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01855CHANNELTSX5270.0 Nucleoside-specific channel-forming protein Tsx signa...
		>CHANNELTSX#Nucleoside-specific channel-forming protein Tsx

signature.
Length = 294

Score = 527 bits (1358), Expect = 0.0
Identities = 257/294 (87%), Positives = 273/294 (92%)

Query: 1 MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60
MKKTLLAAGAV+ALS++F AAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE
Sbjct: 1 MKKTLLAAGAVVALSTTFAAGAAENDKPQYLSDWWHQSVNVVGSYHTRFGPQIRNDTYLE 60

Query: 61 YEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEIEPRFSIDKLTNTDLSFGP 120
YEAFAKKDWFDFYGY DAPVFFGGNS AKGIWN GSPLFMEIEPRFSIDKLTNTDLSFGP
Sbjct: 61 YEAFAKKDWFDFYGYIDAPVFFGGNSTAKGIWNKGSPLFMEIEPRFSIDKLTNTDLSFGP 120

Query: 121 FKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMSLSMNVYAKYQWQNYGAANEN 180
FKEWYFANNYIYDMGRN QSTWYMGLGTDIDTGLPMSLS+NVYAKYQWQNYGA+NEN
Sbjct: 121 FKEWYFANNYIYDMGRNDSQEQSTWYMGLGTDIDTGLPMSLSLNVYAKYQWQNYGASNEN 180

Query: 181 EWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLGDDSGNAINGIKTRTNNSIASSH 240
EWDGYRFK+KYFVP+TDLWGG LSYIGFTNFDWGSDLGDD+ +NG RT+NSIASSH
Sbjct: 181 EWDGYRFKVKYFVPLTDLWGGSLSYIGFTNFDWGSDLGDDNFYDLNGKHARTSNSIASSH 240

Query: 241 ILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGNFNVRSTGWGGYLVVGYNF 294
ILALNY HWHYS+VARY+H+GGQW DDA+LNFG+G F+VRSTGWGGY VVGYNF
Sbjct: 241 ILALNYAHWHYSIVARYFHNGGQWADDAKLNFGDGPFSVRSTGWGGYFVVGYNF 294


7B7485_01910B7485_02035Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_01910015-4.7162542-dehydropantoate 2-reductase
B7485_01915-122-8.219752nucleotide-binding protein
B7485_01920128-8.990684hypothetical protein
B7485_01925132-10.206987hypothetical protein
B7485_01930235-11.811010N-acetyltransferase
B7485_01940127-7.996726GNAT family acetyltransferase
B7485_01945125-0.887501DUF1778 domain-containing protein
B7485_019551230.328353protoheme IX farnesyltransferase
B7485_019601250.744151cytochrome bo(3) ubiquinol oxidase subunit 4
B7485_019653251.194895cytochrome bo(3) ubiquinol oxidase subunit 3
B7485_019703261.056111transposase
B7485_019752261.569839transposase
B7485_019802221.050976general secretion pathway protein GspL
B7485_019853240.829495AmpG family muropeptide MFS transporter
B7485_019900171.482779hypothetical protein
B7485_01995-1180.890062protein BolA
B7485_02000-1160.560709hypothetical protein
B7485_02005015-0.163791trigger factor
B7485_02015121-0.045663ATP-dependent Clp protease proteolytic subunit
B7485_02020330-0.893878ATP-dependent Clp protease ATP-binding subunit
B7485_02025426-0.357506endopeptidase La
B7485_02030328-0.272955DNA-binding protein HU-beta
B7485_02035328-0.007376peptidylprolyl isomerase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02015TCRTETA393e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 39.0 bits (91), Expect = 3e-05
Identities = 71/347 (20%), Positives = 135/347 (38%), Gaps = 20/347 (5%)

Query: 62 KFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLEPGTQLRWMAALAVVIAFCS 121
+F +P++ + F RR LL + V A M W+ + ++A +
Sbjct: 56 QFACAPVLGALSDRF--GRRPVLLVSLAGAAVDYAIMAT----APFLWVLYIGRIVAGIT 109

Query: 122 ASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSGGLALWLADKWLGWQGMYWLMA 181
+ V A+ D+ +ER + GM+ L + ++ A
Sbjct: 110 GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG--FSPHAPFFAAA 167

Query: 182 AL-LIPCIIATLLAPEP--TDTIPVPKTLEQAVVAPLRDFFGRNNAWLILLLIVLYKLGD 238
AL + + L PE + P+ + + + A L+ + ++ +G
Sbjct: 168 ALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQ 227

Query: 239 AFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGALYGGILMQRLSLFRALLIFGIL 298
A +L F +DA +G+ G+L ++ A+ G + RL RAL+ G++
Sbjct: 228 VPA-ALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM-LGMI 285

Query: 299 QGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGTSAFVALLMTLCNKSFSATQFALLS 358
A GY LL+ + + V GG+G A A+L ++ L+
Sbjct: 286 --ADGTGYILLAFATRGWMAFPIMVLL--ASGGIGMPALQAMLSRQVDEERQGQLQGSLA 341

Query: 359 ALSAVGRVYVGPVAGWFVEAHGWSTF--YLFSVAAAVPGLILLLVCR 403
AL+++ + VGP+ + A +T+ + + AA+ L L + R
Sbjct: 342 ALTSLTSI-VGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02020PF06291270.030 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 26.5 bits (58), Expect = 0.030
Identities = 11/34 (32%), Positives = 18/34 (52%)

Query: 3 KKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQ 36
KK+LF ++ GCA+ T+ PT P++
Sbjct: 7 KKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKE 40


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02045HTHFIS290.043 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.043
Identities = 16/73 (21%), Positives = 29/73 (39%), Gaps = 13/73 (17%)

Query: 60 ERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIG 119
E P+ E + ++G+ A + +Y RL D +++ G
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQ----EIYRVLARLMQTD---------LTLMITG 167

Query: 120 PTGSGKTLLAETL 132
+G+GK L+A L
Sbjct: 168 ESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02050GPOSANCHOR350.001 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 35.0 bits (80), Expect = 0.001
Identities = 34/133 (25%), Positives = 69/133 (51%), Gaps = 15/133 (11%)

Query: 191 ERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDVPD- 249
LE A +E + +L R +++ ++ S+ +Q++A ++L E + + +
Sbjct: 291 AALEAEKADLEHQSQVLNAN---RQSLRRDLDASREAK---KQLEAEHQKLEEQNKISEA 344

Query: 250 ENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSPMS-AEATVVRGYIDWMVQVPWNARSK 308
++L+R +DA++ EAK++ EAE QKL+ + +S A +R +D + A+ +
Sbjct: 345 SRQSLRRDLDASR---EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASRE----AKKQ 397

Query: 309 VKKDLRQAQEILD 321
V+K L +A L
Sbjct: 398 VEKALEEANSKLA 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02055DNABINDINGHU1173e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 117 bits (294), Expect = 3e-38
Identities = 49/88 (55%), Positives = 67/88 (76%)

Query: 2 NKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTGR 61
NK LI K+A +++K + A+DA+ ++V+ L +G+ V L+GFG F V+ERAAR GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEITIAAAKVPSFRAGKALKDAV 89
NPQTG+EI I A+KVP+F+AGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


8B7485_02130B7485_02225Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_02130119-3.680173hemolysin expression-modulating protein Hha
B7485_02140119-5.122025Hha toxicity attenuator
B7485_02150218-0.382502multidrug efflux RND transporter permease
B7485_02155116-0.692196MexE family multidrug efflux RND transporter
B7485_02160218-0.571250transcriptional regulator
B7485_02165116-0.062770hypothetical protein
B7485_021701170.318449mechanosensitive channel MscK
B7485_021752130.081009DUF2496 domain-containing protein
B7485_02180115-0.348711primosomal replication protein N''
B7485_021903180.763298hypothetical protein
B7485_022005174.086921adenine phosphoribosyltransferase
B7485_022104253.063764adenine phosphoribosyltransferase
B7485_022204242.654513DNA polymerase III subunit gamma/tau
B7485_022253261.369421YbaB/EbfC family nucleoid-associated protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02170ACRIFLAVINRP13660.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1366 bits (3536), Expect = 0.0
Identities = 800/1033 (77%), Positives = 913/1033 (88%), Gaps = 1/1033 (0%)

Query: 1 MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVTISASYPGADAKTVQDT 60
M NFFI RPIFAWV+AII+M+AG LAIL+LPVAQYPTIAPPAV++SA+YPGADA+TVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADIAQVQVQNKLQLAMPLLPQ 120
VTQVIEQNMNGIDNLMYMSS SDS G+V ITLTF+SGTD DIAQVQVQNKLQLA PLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAANMKDAISRTSGVGDVQLFGS 180
EVQQQG+SVEKSSSS+LMV G ++ + TQ+DISDYVA+N+KD +SR +GVGDVQLFG+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRL 240
QYAMRIW++ + LNK++LTPVDVI +K QN Q+AAGQLGGTP + GQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 TSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYDIIAEFNGQPASGLGIKLATGANAL 300
+ EEFGK+ L+VN DGS V L+DVA++ELGGENY++IA NG+PA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ 360
DTA AI+A+LA+++PFFP G+K++YPYDTTPFV++SIHEVVKTL EAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
N RATLIPTIAVPVVLLGTFA+LAAFG+SINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 AEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGSTGAIYRQFSITIVSAMAL 480
E+ LPPKEAT KSM QIQGALVGIAMVLSAVF+PMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATMLKPIAKGDHGEGKKGFFGWLNRMFEKSTHHYTDSVGGILRSTGR 540
SVLVALILTPALCAT+LKP++ H E K GFFGW N F+ S +HYT+SVG IL STGR
Sbjct: 481 SVLVALILTPALCATLLKPVSAE-HHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 541 YLVLYLIIVVGMAYLFVRLPSSFLPDEDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLT 600
YL++Y +IV GM LF+RLPSSFLP+EDQGVF+TM+QLPAGATQERTQKVL++VT YYL
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 601 KEKNNVESVFAVNGFGFAGRGQNTGIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKD 660
EK NVESVF VNGF F+G+ QN G+AFVSLK W +R G+EN EA+ RA +I+D
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 661 AMVFAFNLPAIVELGTATGFDFELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNG 720
V FN+PAIVELGTATGFDFELIDQAGLGH+ LTQARNQLL AA+HP L SVRPNG
Sbjct: 660 GFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNG 719

Query: 721 LEDTPQFKIDIDQEKAQALGVSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYR 780
LEDT QFK+++DQEKAQALGVS++DIN T+ A GG+YVNDFIDRGRVKK+YV ++AK+R
Sbjct: 720 LEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFR 779

Query: 781 MLPDDIGDWYVRAADGQMVPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEA 840
MLP+D+ YVR+A+G+MVPFSAF++S W YGSPRLERYNGLPSMEI G+AAPG S+G+A
Sbjct: 780 MLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDA 839

Query: 841 MELMEQLASKLPTGVGYDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFS 900
M LME LASKLP G+GYDWTGMSYQERLSGNQAP+L AIS +VVFLCLAALYESWS P S
Sbjct: 840 MALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 901 VMLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGL 960
VMLVVPLG++G LLAAT NDVYF VGLLTTIGLSAKNAILIVEFAKDLM+KEGKG+
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGV 959

Query: 961 IEATLDAVRMRLRPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIF 1020
+EATL AVRMRLRPILMTSLAFILGV+PL IS GAGSGAQNAVG GVMGGMV+AT+LAIF
Sbjct: 960 VEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIF 1019

Query: 1021 FVPVFFVVVRRRF 1033
FVPVFFVV+RR F
Sbjct: 1020 FVPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02175RTXTOXIND447e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.0 bits (104), Expect = 7e-07
Identities = 33/212 (15%), Positives = 71/212 (33%), Gaps = 23/212 (10%)

Query: 100 TYQATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTA 159
+ Y A +L + + Q+ Q +++ ++ L +Q +
Sbjct: 256 EQENKYVEAVNELR--VYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGL 313

Query: 160 AKAAVETARINLAYTKVTSPISGRIGKSNV-TEGALVQNGQATALATVQQLDPIYVDVTQ 218
+ + + +P+S ++ + V TEG +V + T + V + D + V
Sbjct: 314 LTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAE-TLMVIVPEDDTLEVTALV 372

Query: 219 SSNDFLRLKQELA----------NGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVD 268
+ D + KV I D I+ + G + ++++
Sbjct: 373 QNKDIGFINVGQNAIIKVEAFPYTRYGYLV---GKVKNINLDAIEDQRLGLVFNVIISIE 429

Query: 269 QTTGSITLRAIFPNPDHTLLPGMFVRARLEEG 300
+ S + I L GM V A ++ G
Sbjct: 430 ENCLSTGNKNIP------LSSGMAVTAEIKTG 455



Score = 32.9 bits (75), Expect = 0.002
Identities = 26/127 (20%), Positives = 50/127 (39%), Gaps = 10/127 (7%)

Query: 49 PLQITTELPGR-TSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQATYDS 107
++I G+ T + R E++P + I+ + KEG + G L ++ +A
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA---- 134

Query: 108 AKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAAKAAVETA 167
D K Q++ A+L RYQ L + I + + V+ + T+
Sbjct: 135 ---DTLKTQSSLLQARLEQTRYQILSRS--IELNKLPELKLPDEPYFQNVSEEEVLRLTS 189

Query: 168 RINLAYT 174
I ++
Sbjct: 190 LIKEQFS 196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02180HTHTETR2225e-76 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 222 bits (567), Expect = 5e-76
Identities = 215/215 (100%), Positives = 215/215 (100%)

Query: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60
MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120
EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180
GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE 215
APQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE
Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02190RTXTOXIND320.017 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.7 bits (72), Expect = 0.017
Identities = 19/125 (15%), Positives = 40/125 (32%), Gaps = 6/125 (4%)

Query: 28 QNTAFARASSNGDLPTKADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEE 87
N RA L + + L L+ + A L++ ++ E
Sbjct: 207 LNLDKKRAERLTVLARINRYENLSRVEKSR--LDDFSSLLHKQAIAKHAVLEQENKYVEA 264

Query: 88 TVQLRQKVAEAPEKMRQATAALTALSDVDND--EETRKIL--STLSLRQLETRVAQALDD 143
+LR ++ + + +A V E L +T ++ L +A+ +
Sbjct: 265 VNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEER 324

Query: 144 LQNAQ 148
Q +
Sbjct: 325 QQASV 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02220IGASERPTASE397e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 38.9 bits (90), Expect = 7e-05
Identities = 40/251 (15%), Positives = 77/251 (30%), Gaps = 31/251 (12%)

Query: 402 PLPETTSQVLAARQQLQRVQGATKAKKSEPAA----ATRARPVNNAALERLASVTDRVQA 457
P E +Q + + + P+ AR + A + A T
Sbjct: 983 PEVEKRNQTVDTTN----ITTPNNIQADVPSVPSNNEEIARV-DEAPVPPPAPATPSETT 1037

Query: 458 RPVPSALEKAPAKKEAYRWKATTPVMQQKE--------VVATPKALKKA---LEHEKTPE 506
V ++ E AT Q +E V A + + A E ++T
Sbjct: 1038 ETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQT 1097

Query: 507 LAVKLAA---------EAIERDPWAAQVSQLSLPKLVEQVALNAWKE-ESDNAVCLHLRS 556
K A E+ +V+ PK + + E +N ++++
Sbjct: 1098 TETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKE 1157

Query: 557 SQRHLNNRGAQQKLAEALS-MLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARES 615
Q N ++ A+ S ++ E T V N V P A + + +
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSN 1217

Query: 616 IIADNNIQTLR 626
+ + +++R
Sbjct: 1218 KPKNRHRRSVR 1228


9B7485_02300B7485_02365Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_02300-1143.335280glutaminase 1
B7485_023050154.116384amino acid permease
B7485_02310-2182.106011transcriptional regulator
B7485_02315-1180.742764hypothetical protein
B7485_02320020-0.947011paraslipin
B7485_023250190.274134iron ABC transporter ATP-binding protein FetA
B7485_02330-117-0.022271iron export permease FetB
B7485_02335-115-0.460859co-chaperone YbbN
B7485_02340-1170.138634short-chain dehydrogenase/reductase
B7485_023450173.079977ABC transporter ATP-binding protein
B7485_023502144.145798tRNA 2-selenouridine(34) synthase MnmH
B7485_023551133.860659transcriptional regulator
B7485_023601143.497907ureidoglycolate lyase
B7485_023650143.446430transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02310BLACTAMASEA280.048 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 27.8 bits (62), Expect = 0.048
Identities = 11/43 (25%), Positives = 18/43 (41%)

Query: 38 GQLAAVAIVTCDGNVYSAGDSDYRFALESISKVCTLALALEDV 80
G++ + + G +A +D RF + S KV L V
Sbjct: 38 GRVGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARV 80


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02355DHBDHDRGNASE755e-18 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 74.7 bits (183), Expect = 5e-18
Identities = 47/212 (22%), Positives = 80/212 (37%), Gaps = 7/212 (3%)

Query: 16 KSVLITGCSSGIGLESALELKRQGFHVLAGCRKPNDVERMNN----MGFT--GVLIDLDS 69
K ITG + GIG A L QG H+ A P +E++ + D+
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68

Query: 70 PESVNRAADEVIALTDNCLYGIFNNAGFGMYGPLSTISRAQMEQQFSANFFGAHQLTMRL 129
+++ + + + N AG G + ++S + E FS N G + +
Sbjct: 69 SAAIDEITARIEREMGP-IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 130 LPAMLPHGEGRIVMTSSVMGLISTPGRGAYAASKYALEAWSDALRMELRHSGIKVSLIEP 189
M+ G IV S + AYA+SK A ++ L +EL I+ +++ P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 190 GPIRTRFTDNVNQTQSDKPVENPGIAARFTLG 221
G T ++ ++ G F G
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTG 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02365PF05272290.014 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.014
Identities = 12/20 (60%), Positives = 13/20 (65%)

Query: 41 LVGESGSGKSTLLAILAGLD 60
L G G GKSTL+ L GLD
Sbjct: 601 LEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02395PF09025280.020 YopR Core
		>PF09025#YopR Core

Length = 143

Score = 28.1 bits (62), Expect = 0.020
Identities = 17/61 (27%), Positives = 25/61 (40%), Gaps = 8/61 (13%)

Query: 126 EAVLIGQLECKSMVRMCAPLGSR--------LPLHASGAGKALLYPLAEEELMSIILQTG 177
+ + +LE K+M+R PLG + L G L LA EL +I G
Sbjct: 68 QGLEADRLELKAMLRAELPLGRQQQTFLLQLLGAVEHAPGGEYLAQLARRELQVLIPLNG 127

Query: 178 L 178
+
Sbjct: 128 M 128


10B7485_02420B7485_02500Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_024200173.656916metal-dependent hydrolase
B7485_024251184.553128ribosome-associated protein
B7485_024301173.388508bifunctional methylenetetrahydrofolate
B7485_024351212.846020fimbrial protein
B7485_024402202.103100molecular chaperone
B7485_024453201.359055hypothetical protein
B7485_024503201.633427transposase
B7485_024552180.367365*AraC family transcriptional regulator
B7485_02465-121-3.279870hypothetical protein
B7485_02470-121-3.031297hypothetical protein
B7485_02475-122-3.536951bacteriophage N4 adsorption protein B
B7485_02480129-6.068768two-component sensor histidine kinase
B7485_02490022-4.824243DNA-binding response regulator
B7485_02495-123-3.823968copper transporter
B7485_02500223-3.806809copper transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02490PF005771352e-39 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 135 bits (341), Expect = 2e-39
Identities = 60/171 (35%), Positives = 91/171 (53%), Gaps = 16/171 (9%)

Query: 11 QRYTWCL------AGICYSSLAILPSFLSY-----AESYFNPAFLLENGTSVADLSRFER 59
QR T CL + L + +F + AE YFNP FL ++ +VADLSRFE
Sbjct: 10 QRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQAVADLSRFEN 69

Query: 60 GNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMPCFNQVLLERIGLNSSAF 119
G P G YRVD++ N+ ++ ++D+ F NTGD G++PC + L +GLN+++
Sbjct: 70 GQELPPGTYRVDIYLNNGYMATRDVTF-----NTGDSEQGIVPCLTRAQLASMGLNTASV 124

Query: 120 PELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIALLSSAHGVMTP 170
+ ++ C+ L + DAT D RLN+TIPQ + + A G + P
Sbjct: 125 SGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPP 175


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02550HTHFIS861e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 85.7 bits (212), Expect = 1e-21
Identities = 35/117 (29%), Positives = 62/117 (52%)

Query: 2 KLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWD 61
+L+ +D+ L + L+ AG+ V + N + GD DL++ D+++PD N +D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 IVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRR 118
++ ++ A +P+L+++A T +K E GA DYL KPF EL+ + L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02560RTXTOXIND362e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.3 bits (84), Expect = 2e-04
Identities = 26/189 (13%), Positives = 61/189 (32%), Gaps = 13/189 (6%)

Query: 254 QAQTVNSGSLQSVKLPA-GLSSQILLQRPDIMEAEHALM-----AANANIGAARAAFFPS 307
+ +SG + +K + +I+++ + + L+ A A+ ++
Sbjct: 87 NGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQS----- 141

Query: 308 ISLTSGISTASSDLSSLFNASSGMWNFIPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYE 367
SL + + + + P F + L + + ++Q
Sbjct: 142 -SLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQN 200

Query: 368 QKIQNAFKEVADALALRQSLDDQISAQQRYLASLQITLQRAWALYQHGAVSYLEVLDAER 427
QK Q + A R ++ +I+ + + L +L A++ VL+ E
Sbjct: 201 QKYQ-KELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQEN 259

Query: 428 SLFATRQTL 436
L
Sbjct: 260 KYVEAVNEL 268


11B7485_02575B7485_02660Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_02575226-1.045964hypothetical protein
B7485_02580226-0.394871IS3 family transposase
B7485_02585-1171.367958hypothetical protein
B7485_02590-2151.117617hok/gef family protein
B7485_02595-2151.665040phosphopantetheinyl transferase
B7485_02605-1171.256988TonB-dependent siderophore receptor
B7485_02610-2170.720900enterochelin esterase
B7485_02620015-2.075423MbtH family protein
B7485_02630322-2.680600enterobactin synthase component F
B7485_02635118-2.208340enterobactin transporter
B7485_02640021-0.798843LPS O-antigen length regulator
B7485_02645326-1.399803iron-enterobactin transporter ATP-binding
B7485_02650430-3.008019iron-enterobactin transporter permease
B7485_02655430-2.660808iron-enterobactin transporter
B7485_02660229-2.436057MFS transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02680HOKGEFTOXIC562e-15 Hok/Gef cell toxic protein family signature.
		>HOKGEFTOXIC#Hok/Gef cell toxic protein family signature.

Length = 52

Score = 56.4 bits (136), Expect = 2e-15
Identities = 18/50 (36%), Positives = 27/50 (54%)

Query: 1 MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVLAYEPKK 50
+ + V+++CLT+L FT L SLCE ++ E A +AYE K
Sbjct: 3 LPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02685ENTSNTHTASED2741e-96 Enterobactin synthetase component D signature.
		>ENTSNTHTASED#Enterobactin synthetase component D signature.

Length = 234

Score = 274 bits (703), Expect = 1e-96
Identities = 105/183 (57%), Positives = 130/183 (71%), Gaps = 1/183 (0%)

Query: 1 MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYA 60
M T+H LPFAGH LH V+FD ++F E DLLWLPH+ +L+ AGRKRK EHLAGRIAAV+A
Sbjct: 1 MLTSHFPLPFAGHRLHIVDFDASSFREHDLLWLPHHDRLRSAGRKRKAEHLAGRIAAVHA 60

Query: 61 LREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQPIGVDIEEIFSAQTATEL 120
LRE G + VP +G+ RQP+WP ++GSISHC TALAV+SRQ IG+DIE+I S TATEL
Sbjct: 61 LREVGVRTVPGMGDKRQPLWPDGLFGSISHCATTALAVISRQRIGIDIEKIMSQHTATEL 120

Query: 121 TDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKA-SEIQTDAGFLDYQIISWNKQQV 179
+II E + L L F LALTLAFSAKES +KA S+ T GF ++ S +
Sbjct: 121 APSIIDSDERQILQASLLPFPLALTLAFSAKESVYKAFSDRVTLPGFNSAKVTSLTATHI 180

Query: 180 IIH 182
+H
Sbjct: 181 SLH 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02740TCRTETA362e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 36.3 bits (84), Expect = 2e-04
Identities = 82/394 (20%), Positives = 145/394 (36%), Gaps = 44/394 (11%)

Query: 27 FISIVSLGLLGVAVPVQIQMMTHSTWQV---GLSVTLTGGAMFVGLMVGGVLADRYERKK 83
+ V +GL+ +P ++ + HS G+ + L F V G L+DR+ R+
Sbjct: 15 ALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRP 74

Query: 84 VILLARGTCGIGFIGLCLNALL--PEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGR 141
V+L + G ++ + P L +Y+ + G + G A A + +
Sbjct: 75 VLL-------VSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVA-GAYIADITDG 126

Query: 142 ENLMQAGAITMLTVRLGSVISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPP 201
+ + G V P++GGL+ GG + + AA L L LP
Sbjct: 127 DERARHFGFMSACFGFGMVAGPVLGGLM---GGFSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 202 PPQPLEHPLK----SLLAGFRFLLASPLLGGLLTMA----------SAVLVLYPALADNW 247
+ PL+ + LA FR+ ++ L+ + +A+ V++ D +
Sbjct: 184 SHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG--EDRF 241

Query: 248 QMSAAQIGFLYAAIP-LGAAIGALTSGKLAHSARPGLLMLLSTLGS---FLAIGLFGLMP 303
A IG AA L + A+ +G +A ++L + ++ +
Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGW 301

Query: 304 MWILGVVCLALFGWLSAVSSLLQYTMLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGG 363
M +V LA G ML Q E G++ G A +G L
Sbjct: 302 MAFPIMVLLASGGIGMPALQ----AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTA 357

Query: 364 LGAMMTPVASASASGFGLLIIGVLLLLVLVELRR 397
+ A + + +G+ + L LL L LRR
Sbjct: 358 IYA----ASITTWNGWAWIAGAALYLLCLPALRR 387


12B7485_02720B7485_02895Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_027202163.895050methionine aminotransferase
B7485_027250165.368236IS3 family transposase
B7485_027300175.590632chromosome partitioning protein ParB
B7485_02735-1185.188255IS3 family transposase
B7485_02740-1184.656955IS3 family transposase
B7485_02745-2194.628894LysR family transcriptional regulator
B7485_02750-2224.898446thiol:disulfide interchange protein DsbG
B7485_02755-1234.805950peroxiredoxin
B7485_02760-1214.648584alkyl hydroperoxide reductase subunit F
B7485_027700183.201776universal stress protein UspG
B7485_02775-1142.427450glutathione-dependent formaldehyde
B7485_027850150.607269hypothetical protein
B7485_02795-124-1.348743nucleoside diphosphate kinase regulator
B7485_02800126-0.871831hypothetical protein
B7485_02805028-1.478133ribonuclease I
B7485_02810028-3.6029082-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A
B7485_02820124-3.556247apo-citrate lyase phosphoribosyl-dephospho-CoA
B7485_02825017-3.495295citrate lyase subunit alpha
B7485_02830-118-3.941449citrate (pro-3S)-lyase subunit beta
B7485_02835-115-3.250122citrate lyase ACP
B7485_02840-117-1.588811[citrate (pro-3S)-lyase] ligase
B7485_02845-121-0.594956aldehyde dehydrogenase
B7485_02855-120-1.478062integrase
B7485_02860118-1.346033pyridoxal phosphatase
B7485_02865119-0.313391molybdenum import ATP-binding protein ModC
B7485_028700181.015214molybdate ABC transporter permease subunit
B7485_02880-2182.587058molybdate ABC transporter substrate-binding
B7485_02890-2203.506893hypothetical protein
B7485_02895-1223.030064multidrug efflux pump accessory protein AcrZ
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02840BCTLIPOCALIN290.014 Bacterial lipocalin signature.
		>BCTLIPOCALIN#Bacterial lipocalin signature.

Length = 171

Score = 28.8 bits (64), Expect = 0.014
Identities = 18/98 (18%), Positives = 39/98 (39%), Gaps = 13/98 (13%)

Query: 30 QGITIIKTFDAPGGMKGYLGKYQDMGVTIYLTPDGKHAISG--YMYNEKGENLSNTLIEK 87
+ + + F+ YLGK+ ++ + G ++ + N+ G ++ N
Sbjct: 21 ESVKPVSDFEL----NNYLGKWYEVARLDHSFERGLSQVTAEYRVRNDGGISVLN----- 71

Query: 88 EIYAPAGREMWQRMEQSHWLLDGKKDAPVIVYVFSDPF 125
Y+ + W+ E + ++G D + V F PF
Sbjct: 72 RGYSEE-KGEWKEAEGKAYFVNGSTDGYLKVSFFG-PF 107


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02910PF03944270.009 delta endotoxin
		>PF03944#delta endotoxin

Length = 633

Score = 27.3 bits (60), Expect = 0.009
Identities = 12/43 (27%), Positives = 24/43 (55%), Gaps = 3/43 (6%)

Query: 21 IAPLDTQDIDLQINSSVEKQFG---DAIRTTILDVLARYNVRG 60
I+P+ ++ Q + + ++FG D++R + ARY +RG
Sbjct: 496 ISPIHATQVNNQTRTFISEKFGNQGDSLRFEQNNTTARYTLRG 538


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02915LPSBIOSNTHSS391e-05 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 38.6 bits (90), Expect = 1e-05
Identities = 14/67 (20%), Positives = 33/67 (49%), Gaps = 2/67 (2%)

Query: 155 NPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSR--FPYEDRLDLVLKGTADIPRLTVHRGS 212
+P T GH +I++ D +++ +++ + + F ++RL+ + K A +P V
Sbjct: 10 DPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLPNAQVDSFE 69

Query: 213 EYIISRA 219
++ A
Sbjct: 70 GLTVNYA 76


13B7485_03125B7485_03345Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_031252262.141528succinate dehydrogenase hydrophobic membrane
B7485_031301303.158387succinate dehydrogenase cytochrome b556 large
B7485_031351283.231750hypothetical protein
B7485_031402262.963828hypothetical protein
B7485_031451242.669938citrate (Si)-synthase
B7485_031500201.599074type 1 fimbrial protein
B7485_03160022-2.214643hypothetical protein
B7485_03165022-3.451701fimbrial protein
B7485_03175015-2.080459hypothetical protein
B7485_03180-116-2.036187hypothetical protein
B7485_03190-118-3.091178AbrB family transcriptional regulator
B7485_03195-117-1.156817endonuclease VII
B7485_03200-217-1.185404LamB/YcsF family protein
B7485_032101141.024619hypothetical protein
B7485_032151152.114800Nif3-like dinuclear metal center hexameric
B7485_032201173.517799dipeptide permease D
B7485_032250162.551439deoxyribodipyrimidine photo-lyase
B7485_032300162.538309hypothetical protein
B7485_03235-1151.742162type I toxin-antitoxin system SymE family toxin
B7485_03240-2141.454853hypothetical protein
B7485_03245015-0.991591type IV secretion protein Rhs
B7485_032501213.214245hypothetical protein
B7485_032551253.657533hypothetical protein
B7485_032603283.862281potassium-transporting ATPase subunit F
B7485_032703314.211447potassium-transporting ATPase A chain
B7485_032753275.253948potassium-transporting ATPase subunit C
B7485_03280-1162.806147DNA-binding response regulator
B7485_03285-1162.888042TonB-dependent receptor
B7485_03290-2194.119104putrescine-ornithine antiporter
B7485_03295-2184.346645phosphoglucomutase, alpha-D-glucose
B7485_03300-2174.035103replication initiation regulator SeqA
B7485_03305-2142.500444alpha/beta hydrolase
B7485_03315-3111.707405LexA regulated protein
B7485_03320-215-0.281178flavodoxin-1
B7485_03325-214-0.944636Fur leader peptide
B7485_03330-214-0.513828transcriptional repressor
B7485_03335-1180.152080hypothetical protein
B7485_033400220.451504outer membrane porin, OprD family
B7485_03345221-2.137128glutamine--tRNA ligase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_03185FIMBRIALPAPE359e-05 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 34.6 bits (79), Expect = 9e-05
Identities = 43/179 (24%), Positives = 74/179 (41%), Gaps = 26/179 (14%)

Query: 14 SLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQDDIDKEVELGQVTTSHINQS-HHSDAV 72
++L + V+AAD + FKG++I C + + EV G + ++ QS +
Sbjct: 15 AVLMSQHVHAADN----LTFKGKLIIPACTVQ----NAEVNWGDIEIQNLVQSGGNQKDF 66

Query: 73 AVDLLLVNCDLENSSNGSGGKISKVAVTFDSSAKTTGADPILNNTSTGEATGVGVRLMNK 132
VD+ NC + + VT S+ TG ++ NTST G+ + L N
Sbjct: 67 TVDM---NCPYS---------LGTMKVTITSNG-QTGNSILVPNTSTASGDGLLIYLYNS 113

Query: 133 DQSNI----VLGTATPDIDLAPTSSEQTLNFFAWMEQIDQATPVTPGAVTANATYVLDY 187
+ S I LG+ + T+ + + +A + + G +A AT V Y
Sbjct: 114 NNSGIGNAVTLGSQVTPGKITGTAPARKITLYAKLGYKGNMQSLQAGTFSATATLVASY 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_03200PF005775380.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 538 bits (1388), Expect = 0.0
Identities = 215/743 (28%), Positives = 342/743 (46%), Gaps = 57/743 (7%)

Query: 3 FNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVLMDYNLFASSYRPQDGSSSTN 62
D Q+LN++IPQA+++ + + PP W G+ L++YN +S + + G +S
Sbjct: 147 AQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHY 206

Query: 63 LNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSG--EISRTYLFRPLPQLGSKLTLGET 120
+G+N GAWRLR + ++ SD + + T+L R + L S+LTLG+
Sbjct: 207 AYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDG 266

Query: 121 DFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGIAQTNATVTISQSGRVIYQKKVP 180
+IFDG ++ GA LASDD MLP RG+AP I GIA+ A VTI Q+G IY VP
Sbjct: 267 YTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVP 326

Query: 181 PGPFIIDDLNQ-SVQGTLDVKVTEEDGRVNNFQVSAASTPFLTRQGQVRYKLAAGQPRPS 239
PGPF I+D+ G L V + E DG F V +S P L R+G RY + AG+ R
Sbjct: 327 PGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSG 386

Query: 240 MSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDDYHSAAMGIGQNMLWLGALSFDVTWA 299
+ Q E FF + + G+ + ++Y G L+ D Y + GIG+NM LGALS D+T A
Sbjct: 387 -NAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA-DRYRAFNFGIGKNMGALGALSVDMTQA 444

Query: 300 SSHFDTQQDERGLSYRFNYSKQVDATNSTISLAAYRFSDRHFHSYANYLDHKYND----- 354
+S G S RF Y+K ++ + + I L YR+S + ++A+ + N
Sbjct: 445 NSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIET 504

Query: 355 ---------------SDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWNADASTTANI 399
+ A +++ + L+V Q + LY + HQT+W
Sbjct: 505 QDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL-GRTSTLYLSGSHQTYWGTSNVDE-QF 562

Query: 400 TAGFNVDIGDWRDISISTSFNTTHYE-DKDRDNQIYLSISLPFGNGGR-----------V 447
AG N + DI+ + S++ T K RD + L++++PF + R
Sbjct: 563 QAGLNT---AFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASA 619

Query: 448 GYDMQNSSHS-TTHRMSWNDTLDERN--SWGMSAGL-QSDRPDNGAQVSGNYQHLSSAGE 503
Y M + + T+ TL E N S+ + G ++G+ + G
Sbjct: 620 SYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGN 679

Query: 504 WDISGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLD 563
+I ++ ++D + SG A G + N+ ++V G D V+
Sbjct: 680 ANIGYSH-SDDIKQLYYGVSGGVLAHANGVTLGQP--LNDTVVLVKAPGAKDAKVENQTG 736

Query: 564 Y-TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK 622
T+ G AV+P + Y+ + VA++ N L D V + V GAI +R G
Sbjct: 737 VRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGI 796

Query: 623 DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWG--DSQH 680
+ + + + + P GA + +S S G+V + G +LSG+ K V WG ++ H
Sbjct: 797 KLLMTLT-HNNKPLPFGAMV-TSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAH 854

Query: 681 CSLH--LPEH-MEDTANRLILPC 700
C + LP + +L C
Sbjct: 855 CVANYQLPPESQQQLLTQLSAEC 877


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_03320HTHFIS957e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 95.3 bits (237), Expect = 7e-25
Identities = 37/133 (27%), Positives = 60/133 (45%), Gaps = 1/133 (0%)

Query: 2 TNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATRKPDLIILDLGLPDGDGI 61
+L+ +D+ AIR L AL G V A DL++ D+ +PD +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 62 EFIRDLRQWSP-VPVIVLSARSEESDKIAALDAGADDYLSKPFGIGELQARLRVALRRHS 120
+ + +++ P +PV+V+SA++ I A + GA DYL KPF + EL + AL
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 121 ATAAPDPLVKFSG 133
+ G
Sbjct: 124 RRPSKLEDDSQDG 136


14B7485_03610B7485_04340Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_03610015-5.067435twin-arginine translocase subunit TatA
B7485_03615014-3.903609hydrolase
B7485_03620-115-3.615231fluoride ion transporter CrcB
B7485_03625-214-2.224978cold-shock protein CspE
B7485_03630-115-1.902658phospholipid:lipid A palmitoyltransferase
B7485_03635016-0.543956hypothetical protein
B7485_03640015-0.965110C4-dicarboxylate ABC transporter
B7485_03645020-4.048893anaerobic C4-dicarboxylate transporter DcuC
B7485_03655215-4.757599transcriptional regulator
B7485_03660314-4.539039DNA-binding protein
B7485_03665419-4.790673transcriptional regulator
B7485_03670521-4.787810transposase
B7485_03675218-2.708705IS3 family transposase
B7485_03680019-2.229684hypothetical protein
B7485_03685124-2.764169hypothetical protein
B7485_03690121-2.053427hypothetical protein
B7485_03695119-1.069087transposase
B7485_03700017-0.681036hypothetical protein
B7485_037101200.426981phage head morphogenesis protein
B7485_037151200.989651hypothetical protein
B7485_037202201.229030terminase
B7485_037251200.533782hypothetical protein
B7485_037351190.764278DedA family protein
B7485_037400190.333401IS3 family transposase
B7485_03750025-1.811833IS3 family transposase
B7485_03755026-1.786086integrase
B7485_03760129-2.432960acyl-CoA thioesterase
B7485_03770338-3.203336hydratase
B7485_03775226-1.239677anion permease
B7485_03780-116-1.086943isomerase
B7485_03785-112-1.2122556-phosphogluconolactonase
B7485_03790-112-1.673145DNA invertase
B7485_03795013-2.314573invasion protein
B7485_03800213-1.281193hypothetical protein
B7485_03805212-1.948197hypothetical protein
B7485_03810117-3.472970exodeoxyribonuclease VIII
B7485_03815223-3.375312IS3 family transposase
B7485_03820223-2.787939hypothetical protein
B7485_03825322-1.677849transcriptional regulator
B7485_03830529-3.452942hypothetical protein
B7485_03835627-3.064367hypothetical protein
B7485_03840321-1.749158hypothetical protein
B7485_03845222-2.841716DUF1391 domain-containing protein
B7485_03850223-3.266153transcriptional regulator
B7485_03855222-3.676166transcriptional regulator
B7485_03860123-1.574417hypothetical protein
B7485_03870027-1.641420hypothetical protein
B7485_03875026-0.841688hypothetical protein
B7485_03880030-1.471229hypothetical protein
B7485_03885129-1.351006Hok/Gef family protein
B7485_03890426-3.086599hypothetical protein
B7485_03895527-2.549028hypothetical protein
B7485_03900322-1.290217IS3 family transposase
B7485_03905320-1.086474DUF1364 domain-containing protein
B7485_03910222-0.956025transposase
B7485_03915120-1.182402IS3 family transposase
B7485_03920223-1.486569hypothetical protein
B7485_03925123-2.393961****lysis protein S
B7485_03935226-3.683724IS3 family transposase
B7485_03940124-3.160710hypothetical protein
B7485_03945126-3.026315IS3 family transposase
B7485_03950123-3.386092hypothetical protein
B7485_03955224-1.432726hypothetical protein
B7485_03960225-2.208155protein ninB
B7485_03965329-2.431507NinE family protein
B7485_03970332-2.315922transcriptional regulator
B7485_03975329-2.440120hypothetical protein
B7485_03980330-2.213019hypothetical protein
B7485_03985327-2.459613hypothetical protein
B7485_04010326-1.309746hypothetical protein
B7485_04015326-1.264061DNA-binding protein
B7485_04020326-1.613551AAA family ATPase
B7485_04025329-1.595440replicative DNA helicase
B7485_04030329-1.704365hypothetical protein
B7485_04035230-1.989323protein ninH
B7485_04040333-3.234928antitermination protein
B7485_04045234-3.828811****holin
B7485_04050234-2.747807hypothetical protein
B7485_04055131-2.001459lysozyme
B7485_04060229-1.910424lysis protein
B7485_04065125-1.708808IS3 family transposase
B7485_04070321-0.871607transcriptional regulator
B7485_04075221-0.571725hypothetical protein
B7485_04080221-0.815083hypothetical protein
B7485_04085221-1.263220protein convertase
B7485_04090422-0.924445hypothetical protein
B7485_04095423-1.439157IS3 family transposase
B7485_04100123-2.576305DNA packaging protein
B7485_04105325-2.152016terminase
B7485_04110326-1.967133phage tail protein
B7485_04135226-1.863140scaffolding protein
B7485_04140325-1.968606head decoration protein
B7485_04145227-1.747620minor capsid protein E
B7485_04150428-1.800049DNA-packaging protein FI
B7485_04155526-2.310027phage tail protein
B7485_04160625-2.381965phage tail protein
B7485_04165530-1.221242phage tail protein
B7485_041703270.349422phage tail protein
B7485_041753280.134513phage minor tail protein G
B7485_04185321-0.503623phage tail assembly protein T
B7485_04190221-0.174810phage tail tape measure protein
B7485_041951230.984024phage tail protein
B7485_042001192.057765phage minor tail protein L
B7485_042051212.882423phage tail protein
B7485_042151234.770590tail assembly protein
B7485_042254264.070492host specificity protein J
B7485_042304282.306694Ail/Lom family protein
B7485_042355232.345323hypothetical protein
B7485_042406262.193007hypothetical protein
B7485_042503253.421383invasion plasmid antigen
B7485_042602253.598757hypothetical protein
B7485_042653284.092937kinase inhibitor
B7485_042703274.481271adenosylmethionine--8-amino-7-oxononanoate
B7485_042752264.370926biotin synthase
B7485_042805264.6125768-amino-7-oxononanoate synthase
B7485_042854244.462324malonyl-[acyl-carrier protein]
B7485_042904234.185022dethiobiotin synthase
B7485_042954233.907648excinuclease ABC subunit B
B7485_043055211.171416hypothetical protein
B7485_043106250.404620GTP 3',8-cyclase MoaA
B7485_04315522-0.559880molybdenum cofactor biosynthesis protein
B7485_043201170.642046cyclic pyranopterin monophosphate synthase MoaC
B7485_04325-1131.024284molybdopterin synthase sulfur carrier subunit
B7485_043303173.739724molybdenum cofactor biosynthesis protein MoaE
B7485_043402154.138435hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_03860CHANLCOLICIN280.031 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 28.1 bits (62), Expect = 0.031
Identities = 29/116 (25%), Positives = 48/116 (41%), Gaps = 4/116 (3%)

Query: 96 DDVHHQDNAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLL-HIFTYAAGDRK 154
+H +D E K LAG + E AQA A D V + R L F A R
Sbjct: 221 SSIHARD--AEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRV 278

Query: 155 YLHHATREQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAE 210
E++K +TA E + N ++ + +++ + NA + R+ +A +
Sbjct: 279 GAGKIREEKQKQVTASE-TRINRINADITQIQKAISQVSNNRNAGIARVHEAEENL 333


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_03945HOKGEFTOXIC624e-17 Hok/Gef cell toxic protein family signature.
		>HOKGEFTOXIC#Hok/Gef cell toxic protein family signature.

Length = 52

Score = 61.8 bits (150), Expect = 4e-17
Identities = 20/48 (41%), Positives = 34/48 (70%)

Query: 23 QKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESRK 70
+ +++ ++++CLT+++ +TRK LCE+R R G EVA F+ YES K
Sbjct: 5 RSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04030PF05272541e-09 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 53.9 bits (129), Expect = 1e-09
Identities = 30/82 (36%), Positives = 48/82 (58%), Gaps = 2/82 (2%)

Query: 4 SELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNSLKVNLIGKKKWADFAEGDG 63
+ L+D L + + P LP G + GHE+ G++ G KG+S KVN + KW DF+ G+
Sbjct: 12 TSLADALLTRAKDLLPEWLPGGVLVGHEYECGSLAGGKGDSCKVN-VTTGKWCDFSTGES 70

Query: 64 G-DMLDLWMACRGINLHQAMQE 84
G D+LDL+ G+ + +A +
Sbjct: 71 GRDLLDLYAEIHGLKVSKAAAQ 92


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04105DNABINDNGFIS303e-04 DNA-binding protein FIS signature.
		>DNABINDNGFIS#DNA-binding protein FIS signature.

Length = 98

Score = 29.6 bits (66), Expect = 3e-04
Identities = 12/33 (36%), Positives = 19/33 (57%)

Query: 3 VKIQTIPELLIQTRGNMTEVSRMLNCNRATVRK 35
V+ + ++ TRGN T + M+ NR T+RK
Sbjct: 58 VEQPLLDMVMQYTRGNQTRAALMMGINRGTLRK 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04110FIMREGULATRY270.014 Escherichia coli: P pili regulatory PapB protein si...
		>FIMREGULATRY#Escherichia coli: P pili regulatory PapB protein

signature.
Length = 104

Score = 26.8 bits (59), Expect = 0.014
Identities = 8/31 (25%), Positives = 15/31 (48%)

Query: 71 LVDYYVFGMTFMTLARKHGCSDGYIGKKLQK 101
+ DY V G + + K+ ++GY L +
Sbjct: 51 MKDYLVGGHSRKEVCEKYQMNNGYFSTTLGR 81


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04275RTXTOXIND396e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 39.4 bits (92), Expect = 6e-05
Identities = 14/194 (7%), Positives = 43/194 (22%), Gaps = 12/194 (6%)

Query: 29 LNGAASDAERSSARMQRFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMARE 88
L ++A+ + Q + + Q S + + E
Sbjct: 127 LTALGAEADTLKTQSSL---LQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEE 183

Query: 89 VEQTRLRVDALNQKMREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQI 148
V + + + ++ Q + + +I+ + +
Sbjct: 184 VLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDD---F 240

Query: 149 RQARNSGGVGQQDYLALISEITAKTRALTQAE------EQATRQKAAFIRQLKEQATRQN 202
+ + + L ++ L + E + + + +
Sbjct: 241 SSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEI 300

Query: 203 LSSSELLRARAAQL 216
L L
Sbjct: 301 LDKLRQTTDNIGLL 314



Score = 38.7 bits (90), Expect = 1e-04
Identities = 25/224 (11%), Positives = 63/224 (28%), Gaps = 30/224 (13%)

Query: 545 NYQEQQKRRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAI 604
+ + + A L + + E+ ++ ++ D+ + E R I
Sbjct: 133 EADTLKTQSSLLQARLEQ-TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLI 191

Query: 605 KKNTRATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISD 664
K+ + Q+ Q E + R R+ + R+ D
Sbjct: 192 KEQ------------FSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDD 239

Query: 665 LDGKKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERA 724
L + I +L+ + + ++ L + + Q+ S++ +
Sbjct: 240 F--SSL---------LHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEE 288

Query: 725 LREQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSR 768
+ + + Q I +L + E +
Sbjct: 289 YQ-LVTQLFKN----EILDKLRQTTDNIGL-LTLELAKNEERQQ 326



Score = 32.5 bits (74), Expect = 0.010
Identities = 31/238 (13%), Positives = 69/238 (28%), Gaps = 42/238 (17%)

Query: 402 DPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETGKRTSDIDNNLN 461
+ A L +LEQ R RS + ++ L +E + + L
Sbjct: 128 TALGAEADTLKTQSSLLQARLEQTRYQILS-RSIELNKLPELKLPDEPYFQNVSEEEVLR 186

Query: 462 ALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYPWTNASTQVEYDQQ 521
+ S W Q + +N+ D A+ + +I R + ++
Sbjct: 187 LTSLIKEQFSTWQNQKYQKELNL---DKKRAERLTVLARINRYEN--------LSRVEKS 235

Query: 522 RLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNETEAARHQREIARINAM 581
RL+D L +A A+ EQ+ + L +++ +
Sbjct: 236 RLDDFSS------LLHKQAIAKHAVLEQENKYVEAVNELRVY-----------KSQLEQI 278

Query: 582 QYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYSQQQAQVEGQIAAARQ 639
+ + ++ + + K + T + ++A +
Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTT-------------DNIGLLTLELAKNEE 323



Score = 31.0 bits (70), Expect = 0.030
Identities = 26/185 (14%), Positives = 57/185 (30%), Gaps = 13/185 (7%)

Query: 13 IDAAEFKNEIPRIKNLLNGAASDAERSSARMQRFMERQTQAARQTMQAASSAATAASAHA 72
+ E + +E R+ ++ Q + A
Sbjct: 157 SRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAER 216

Query: 73 QTVEKNARAHERMAREVEQTRLRVDALNQKMREEQAQARALAEAQDKAAAAFYRQIDSVK 132
TV +E ++R VE++RL + +QA A+ Q+ ++
Sbjct: 217 LTVLARINRYENLSR-VEKSRL---DDFSSLLHKQAIAKHAVLEQENKYVEAVNEL---- 268

Query: 133 QAGAGLQELQRIQQQIRQARNSGGVGQQDYLALISEITAKTRA-LTQAEEQATRQKAAFI 191
+L++I+ +I A+ + Q + I + +T + + K
Sbjct: 269 --RVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLE--LAKNEER 324

Query: 192 RQLKE 196
+Q
Sbjct: 325 QQASV 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04305ENTEROVIROMP1342e-42 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 134 bits (338), Expect = 2e-42
Identities = 63/200 (31%), Positives = 101/200 (50%), Gaps = 30/200 (15%)

Query: 1 MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPGSDDLNGINVKYRYEFT 60
M+K+ A + + A LA + + A+ ST++ GY + + + G N+KYRYE
Sbjct: 1 MKKI-ACLSALAAVLAFTAGTSVAA--TSTVTGGYAQSDAQGQMNK-MGGFNLKYRYEED 56

Query: 61 DT-LGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGV 119
++ LG++ SF+Y T S T D +N+++ + AG + R+N+W S Y + GV
Sbjct: 57 NSPLGVIGSFTY--------TEKSRTASSGDYNKNQYYGITAGPAYRINDWASIYGVVGV 108

Query: 120 AYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYE 179
Y + T T+ HD S+ ++GAG+QFNP E+VA+D +YE
Sbjct: 109 GYGKFQT--------TEYPTYKHD---------TSDYGFSYGAGLQFNPMENVALDFSYE 151

Query: 180 GSGSGDWRTDGFIVGVGYKF 199
S +I GVGY+F
Sbjct: 152 QSRIRSVDVGTWIAGVGYRF 171


15B7485_04615B7485_04665Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_04615-2163.568402c-di-GMP phosphodiesterase
B7485_04620-1123.264927GGDEF domain-containing protein
B7485_04625-1132.69753130S ribosomal protein S12 methylthiotransferase
B7485_04630-1132.565684biofilm regulator BssR
B7485_046350152.228960dehydrogenase
B7485_04645116-3.189872aldose sugar dehydrogenase
B7485_04650113-3.674452glutathione S-transferase
B7485_04655011-4.521279serine-type D-Ala-D-Ala carboxypeptidase
B7485_04660110-4.638839transposase
B7485_04665110-5.473144DNA-binding transcriptional repressor DeoR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04700BLACTAMASEA421e-06 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 42.1 bits (99), Expect = 1e-06
Identities = 41/201 (20%), Positives = 64/201 (31%), Gaps = 34/201 (16%)

Query: 16 AFLFLFAPTAFAAEQTVEAPSVDARAW----------ILMDYASGKVLAEGNADEKLDPA 65
+ L A A P + I MD ASG+ L ADE+
Sbjct: 7 CIISLLATLPLAV-HASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMM 65

Query: 66 SLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGNPALRGSSVMFLKPGDQVSVADLN 125
S K++ V + A +L + + +P V D ++V +L
Sbjct: 66 STFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSP------VSEKHLADGMTVGELC 119

Query: 126 KGVIIQSGNDACIALADYVAGSQESFIGLMNGYAKKLGLTNTT---FQTVHGLDAPGQF- 181
I S N A L V G + + +++G T ++T PG
Sbjct: 120 AAAITMSDNSAANLLLATVGGPAG-----LTAFLRQIGDNVTRLDRWETELNEALPGDAR 174

Query: 182 --STARDMA------LLGKAL 194
+T MA L + L
Sbjct: 175 DTTTPASMAATLRKLLTSQRL 195


16B7485_04825B7485_04870Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_04825219-0.079801hypothetical protein
B7485_04830019-7.441075N-acetylmuramoyl-L-alanine amidase
B7485_04835024-9.459009hypothetical protein
B7485_04845126-8.820330NAD(P)-dependent oxidoreductase
B7485_04850121-7.555113low-specificity L-threonine aldolase
B7485_04855116-4.661279pyruvate oxidase
B7485_048600122.593606hybrid-cluster NAD(P)-dependent oxidoreductase
B7485_048650142.890664hydroxylamine reductase
B7485_04870-1133.023379hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04870ECOLIPORIN300.010 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 29.9 bits (67), Expect = 0.010
Identities = 21/54 (38%), Positives = 27/54 (50%), Gaps = 9/54 (16%)

Query: 2 RRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRIKVLVIHYTADD 55
R+V LV ALL AG A I K+G +LD Y ++ L HY +DD
Sbjct: 3 RKVLALVIPALLAAGAAHAAEIYNKDGNKLDL-------YGKVDGL--HYFSDD 47


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04875NUCEPIMERASE738e-17 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 73.3 bits (180), Expect = 8e-17
Identities = 70/363 (19%), Positives = 123/363 (33%), Gaps = 65/363 (17%)

Query: 1 MKVLVTGATSGLGRNAVEFLCQKGISVRA---------SGRNEAMGKLLEKMGAEFVPTD 51
MK LVTGA +G + + L + G V +A +LL + G +F D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 52 LTELVSSQAKVMLAGIDTLWHCS-------SFTSPWGTQQAFDLANVRATRRLGEWAVAW 104
L + + ++ S +P A+ +N+ + E
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPH----AYADSNLTGFLNILEGCRHN 116

Query: 105 GVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQTRFT 164
+++ ++ SS S+Y + D + +A +K A+E + + S T
Sbjct: 117 KIQHLLYASSSSVYGL-NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH-LYGLPAT 174

Query: 165 ILRPQSLFGPHDK--VFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLASQEA 222
LR +++GP + + + + M SI + + G D TY ++ A+
Sbjct: 175 GLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVI 234

Query: 223 CDKLPS--------------GRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLD 268
RVYNI N L +Q L D L I+ + +P D
Sbjct: 235 PHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGD 294

Query: 269 MIARSMERLGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAW 328
+ T D E +G+ P T+ +G++ W
Sbjct: 295 V----------------LETS-----------ADTKALYEVIGFTPETTVKDGVKNFVNW 327

Query: 329 LRD 331
RD
Sbjct: 328 YRD 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04880NUCEPIMERASE562e-10 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 55.6 bits (134), Expect = 2e-10
Identities = 29/125 (23%), Positives = 52/125 (41%), Gaps = 17/125 (13%)

Query: 4 RILVLGASGYIGQHLVRTLSQQGHQILA---------AARHVDRLAKLQLANVSCHKVDL 54
+ LV GA+G+IG H+ + L + GHQ++ + RL L HK+DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 SWPDNLPALLQD--IDTVYFLVH------SMGEGGDFIAQERQLALNVRDALREVPVKQL 106
+ + + L + V+ H S+ + LN+ + R ++ L
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 107 IFLSS 111
++ SS
Sbjct: 122 LYASS 126


17B7485_05065B7485_05130Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_05065323-0.137645formate transporter FocA
B7485_050704230.46029130S ribosomal protein S12 methylthiotransferase
B7485_050753200.672903phosphoserine transaminase
B7485_050803210.3973823-phosphoshikimate 1-carboxyvinyltransferase
B7485_05085224-0.816039IS4 family transposase
B7485_05095126-3.078333metalloprotease
B7485_05100127-3.466224cytidylate kinase
B7485_05105124-2.59852830S ribosomal protein S1
B7485_05110119-3.204262integration host factor subunit beta
B7485_05115429-1.818291lipid A export ATP-binding/permease MsbA
B7485_05120428-1.816992tetraacyldisaccharide 4'-kinase
B7485_05125229-1.470951winged helix-turn-helix domain-containing
B7485_05130227-1.165476hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_05190DNABINDINGHU1172e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 117 bits (296), Expect = 2e-38
Identities = 33/88 (37%), Positives = 57/88 (64%), Gaps = 1/88 (1%)

Query: 2 TKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIEIRGFGSFSLHYRAPRTG 61
K +LI ++A + + + K AV + ++S LA+GE++++ GFG+F + RA R G
Sbjct: 3 NKQDLIAKVA-EATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKG 61

Query: 62 RNPKTGDKVELEGKYVPHFKPGKELRDR 89
RNP+TG++++++ VP FK GK L+D
Sbjct: 62 RNPQTGEEIKIKASKVPAFKAGKALKDA 89


18B7485_05610B7485_05725Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_05610228-0.791199pyrimidine utilization protein D
B7485_05615129-1.198754pyrimidine utilization protein C
B7485_056201220.595866pyrimidine utilization protein B
B7485_05625-2190.092211pyrimidine monooxygenase RutA
B7485_05630-1130.242078transcriptional regulator
B7485_05640-2142.051492IS91 family transposase
B7485_05645-1132.041241transposase
B7485_056550152.115107iron permease
B7485_05665-220-5.194544iron uptake system component EfeO
B7485_05675-218-5.156135deferrochelatase/peroxidase EfeB
B7485_05680018-4.645577phosphate starvation protein PhoH
B7485_05690017-4.184002poly-beta-1,6-N-acetyl-D-glucosamine
B7485_057001182.769843*phosphatase
B7485_057100193.872277molecular chaperone
B7485_057150183.902931hypothetical protein
B7485_05720-1194.128037curli production assembly/transport component
B7485_057250153.035632curli assembly protein CsgE
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_05740ISCHRISMTASE726e-17 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 71.6 bits (175), Expect = 6e-17
Identities = 43/176 (24%), Positives = 70/176 (39%), Gaps = 23/176 (13%)

Query: 12 TFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQ 71
DP ++ ++ DMQN + +D S + ANI+ G+ +++
Sbjct: 25 VPDPNRAVLLIHDMQNYF------VDAFTAGASPVTELSANIRKLKNQCVQLGIPVVY-- 76

Query: 72 NGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVL 131
PGS N L G L G ++ +++ EL P+ D+VL
Sbjct: 77 ---------TAQPGSQNPDDRALLTDF------WGPGLNSGPYEEKIITELAPEDDDLVL 121

Query: 132 PKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEHFGVVLEDA 187
K RYS F T L ++R G L+ TGI ++ T + F + + DA
Sbjct: 122 TKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDA 177


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_05750HTHTETR662e-15 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 65.8 bits (160), Expect = 2e-15
Identities = 30/165 (18%), Positives = 62/165 (37%), Gaps = 8/165 (4%)

Query: 10 GKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQIAELAGVSKTNLLYYFPSKEALYIAV 69
K + ++ IL AL FSQ G T L +IA+ AGV++ + ++F K L+ +
Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62

Query: 70 LRQILDIWLAPLKAFREDF--APLAAIKEYIRLKLEVSRDYPQASRLFCM-----EMLAG 122
++ F PL+ ++E + LE + + L + E +
Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122

Query: 123 APLLMDELTGDLKALIDEKSALIAGWVKSGKL-APIDPQHLIFMI 166
++ D + +++ L A + + ++
Sbjct: 123 MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM 167


19B7485_05790B7485_06010Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_05790-223-3.145340hypothetical protein
B7485_05795-221-2.360561hypothetical protein
B7485_05800021-3.317373glucan biosynthesis protein G
B7485_05805-118-2.216138glucan biosynthesis protein H
B7485_05810016-0.496698IS91 family transposase
B7485_05825-118-1.878012transposase
B7485_05835020-4.352976IS3 family transposase
B7485_05840222-6.270191resolvase
B7485_05845129-8.283796IS3 family transposase
B7485_05850133-9.713400IS3 family transposase
B7485_05855029-6.780958transposase
B7485_05860128-5.807749multidrug transporter
B7485_05865233-5.049360lipid A biosynthesis lauroyl acyltransferase
B7485_05870330-4.615062sulfurtransferase
B7485_05875328-2.785545hypothetical protein
B7485_05880-122-2.262202cytochrome b
B7485_05885021-4.066716DUF2770 domain-containing protein
B7485_05890123-4.272506N-methyl-L-tryptophan oxidase
B7485_05895119-3.825981transcriptional regulator
B7485_05900-115-3.099278DNA-damage-inducible protein I
B7485_05905-213-1.136843dihydroorotase
B7485_05915-1151.194875lipoprotein
B7485_059200150.034813glutaredoxin 2
B7485_059250140.120236MFS transporter
B7485_05935326-1.37862230S ribosomal protein S5 alanine
B7485_05940232-3.130502DUF480 domain-containing protein
B7485_05945332-2.698711gfo/Idh/MocA family oxidoreductase
B7485_05950431-2.080889lipid II flippase MurJ
B7485_05955332-1.616824flagellar biosynthesis protein FlgN
B7485_05960431-0.698335anti-sigma-28 factor FlgM
B7485_05965430-0.698335flagella basal body P-ring formation protein
B7485_05970430-0.523217flagellar basal body rod protein FlgB
B7485_05980530-1.519263flagellar basal body rod modification protein
B7485_05985325-1.918763flagellar hook protein FlgE
B7485_05990020-1.571655flagellar basal-body rod protein FlgG
B7485_05995018-2.988311flagellar L-ring protein
B7485_06000-214-2.941494flagellar P-ring protein
B7485_06010216-1.354538flagellar assembly peptidoglycan hydrolase FlgJ
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06005TCRTETA270.004 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 27.1 bits (60), Expect = 0.004
Identities = 11/38 (28%), Positives = 14/38 (36%)

Query: 14 RNLIVAWLGCFLTGAAFSLVMPFLPLYVEQLGVTGHSA 51
R LIV L L+MP LP + L +
Sbjct: 5 RPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVT 42


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06065TCRTETA538e-10 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 53.3 bits (128), Expect = 8e-10
Identities = 61/356 (17%), Positives = 121/356 (33%), Gaps = 13/356 (3%)

Query: 14 FLLIDNMLVVLGFFVVFPLIS--IRFVDQMGWAAVMVGIALGLRQFIQQGLGIFGGAIAD 71
+L L +G ++ P++ +R + GI L L +Q GA++D
Sbjct: 9 VILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSD 68

Query: 72 RFGAKPMIVTGMLMRAAGFATMGIAHEPWLLWFSCLLSGLGGTLFDPPRSALVVKLIRPQ 131
RFG +P+++ + A +A M A W+L+ +++G+ G A + +
Sbjct: 69 RFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVA-GAYIADITDGD 127

Query: 132 QRGRFFSLLMMQDSASAVIGALLGSWLLQYDFRLVCATGAVLFVLCAAFNAWLLPAWKLS 191
+R R F + V G +LG + + A L L +LLP
Sbjct: 128 ERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKG 187

Query: 192 TVRTPVREGMTRVMRDKRFVTYVLTLAGYYMLAVQVMLMLPIMV--------NDVAGAPS 243
R P+R + R+ + +A + + L+ + + +
Sbjct: 188 E-RRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDAT 246

Query: 244 AVKWMYAIEACLSLTLLYPIARWSEKHFRLEHRLMAGLLIMSLSMMPVGMVSGLQQLFTL 303
+ A L I LM G++ + + + F +
Sbjct: 247 TIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPI 306

Query: 304 ICLFYIGSIIAEPARETLSASLADARARGSYMGFSRLGLAIGGAIGYIGGGWLFDL 359
+ L G I PA + + + D +G G ++ +G + ++
Sbjct: 307 MVLLASGGIGM-PALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAA 361


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06120FLGHOOKAP1393e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 39.2 bits (91), Expect = 3e-05
Identities = 16/49 (32%), Positives = 28/49 (57%)

Query: 354 TLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR 402
L+N S V+L +E N+ Q+ Y +NAQ ++T + I + +N+R
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 36.9 bits (85), Expect = 1e-04
Identities = 22/56 (39%), Positives = 30/56 (53%), Gaps = 4/56 (7%)

Query: 6 AVSGLNAAATNLDVIGNNIANSATYGFKSGTASFAD----MFAGSKVGLGVKVAGI 57
A+SGLNAA L+ NNI++ G+ T A + AG VG GV V+G+
Sbjct: 7 AMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06130FLGHOOKAP1444e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 43.8 bits (103), Expect = 4e-07
Identities = 18/81 (22%), Positives = 36/81 (44%), Gaps = 14/81 (17%)

Query: 3 SSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDLLYQTIRQPGAQSSEQTTL 62
S + A +GL+A Q ++ +NN+++ + G+ RQ + + +TL
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI--------------MAQANSTL 47

Query: 63 PSGLQIGTGVRPVATERLHSQ 83
+G +G GV +R +
Sbjct: 48 GAGGWVGNGVYVSGVQREYDA 68



Score = 41.1 bits (96), Expect = 3e-06
Identities = 11/41 (26%), Positives = 21/41 (51%)

Query: 220 ETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL 260
S VN+ EE N+ + Q+ Y N++ + T + + L +
Sbjct: 505 SISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06135FLGLRINGFLGH349e-126 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 349 bits (897), Expect = e-126
Identities = 232/232 (100%), Positives = 232/232 (100%)

Query: 1 MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY 60
MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY
Sbjct: 1 MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY 60

Query: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA 120
GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA 120

Query: 121 RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF 180
RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF
Sbjct: 121 RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF 180

Query: 181 SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232
SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM
Sbjct: 181 SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06140FLGPRINGFLGI427e-152 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 427 bits (1100), Expect = e-152
Identities = 157/363 (43%), Positives = 213/363 (58%), Gaps = 9/363 (2%)

Query: 4 FLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVGLDGTGDQTTQTPFTTQTLN 63
F + L A RI+D+ S+Q R N LIGYGLVVGL GTGD +PFT Q++
Sbjct: 13 FSALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMR 72

Query: 64 NMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQTIDVVVSSMGNAKSLRGGTLLM 123
ML LGIT G + KN+AAVMVTA+LPPF G +DV VSS+G+A SLRGG L+M
Sbjct: 73 AMLQNLGITTQGGQS-NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIM 131

Query: 124 TPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQLNGGRITNGAVIERELPSQFGVGN 183
T L G D Q+YA+AQG ++V G A +++ R+ NGA+IERELPS+F
Sbjct: 132 TSLSGADGQIYAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSV 191

Query: 184 TLNLQLNDEDFSMAQQIADTINRVR----GYGSATALDARTIQVRVPSGNSSQVRFLADI 239
L LQL + DFS A ++AD +N G A D++ I V+ P + R +A+I
Sbjct: 192 NLVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPRV-ADLTRLMAEI 250

Query: 240 QNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQANVSQPDTPFGG 299
+N+ V T AKVVIN RTG++V+ +V + AV+ G L+V V V QP PF
Sbjct: 251 ENLTVE-TDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQP-APFSR 308

Query: 300 GQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRALNALGATPMDLMSILQSMQSAGCLR 359
GQT V PQT I Q G + ++ L +V LN++G +++ILQ ++SAG L+
Sbjct: 309 GQTAVQPQTDIMAMQEGSKV-AIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQ 367

Query: 360 AKL 362
A+L
Sbjct: 368 AEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06145FLGFLGJ5030.0 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 503 bits (1297), Expect = 0.0
Identities = 308/313 (98%), Positives = 309/313 (98%)

Query: 1 MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG 60
MISDSKLLASAAWDAQSLNELKAKA EDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG
Sbjct: 1 MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG 60

Query: 61 LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET 120
LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET
Sbjct: 61 LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET 120

Query: 121 VVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPAQLASQQSGVPHHLILAQAAL 180
VVRYQNQ LSQLVQKAVPRNYDDSLPGDS+AFLAQLSLPAQLASQQSGVPHHLILAQAAL
Sbjct: 121 VVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQAAL 180

Query: 181 ESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITTTEYENGEAKKVKAKFRVYSSYL 240
ESGWGQRQIRRENGEPSYNLFGVKASGNWKG VTEITTTEYENGEAKKVKAKFRVYSSYL
Sbjct: 181 ESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYL 240

Query: 241 EALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAGYATDPHYARKLTNMIQQMKSISDK 300
EALSDYVGLLTRNPRYAAVTTAASAEQGAQ LQDAGYATDPHYARKLTNMIQQMKSISDK
Sbjct: 241 EALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKSISDK 300

Query: 301 VSKTYSMNIDNLF 313
VSKTYSMNIDNLF
Sbjct: 301 VSKTYSMNIDNLF 313


20B7485_06080B7485_06160Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_060802161.218324acyl carrier protein
B7485_060851140.721410beta-ketoacyl-[acyl-carrier-protein] synthase
B7485_060901200.693026aminodeoxychorismate lyase
B7485_060952170.862994cell division protein YceG
B7485_061001182.066553thymidylate kinase
B7485_061052172.224828DNA polymerase III subunit delta'
B7485_061103142.195108metal-dependent hydrolase
B7485_061152132.162143PTS glucose transporter subunit IIBC
B7485_061200132.093805histidine triad nucleotide-binding protein
B7485_06130-1110.822929hypothetical protein
B7485_061350111.931469penicillin-binding protein activator LpoB
B7485_061400121.588949thiamine kinase
B7485_061501121.030386beta-hexosaminidase
B7485_061553150.987941hypothetical protein
B7485_061604171.418462FAD-dependent oxidoreductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06210TYPE4SSCAGA280.005 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 27.7 bits (61), Expect = 0.005
Identities = 16/48 (33%), Positives = 25/48 (52%)

Query: 10 KIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPD 57
++ G Q + QEE+ N F+E L ++ L +E+F TEI D
Sbjct: 380 QLTGSQRALSQEEIQNKIDFMEFLAQNNAKLDNLSEKEKEKFRTEIKD 427


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06270TONBPROTEIN310.002 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 31.5 bits (71), Expect = 0.002
Identities = 23/124 (18%), Positives = 38/124 (30%), Gaps = 7/124 (5%)

Query: 25 EPAPVEEVKPAPEQPAEPQQPVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAM 84
+P V V PA +P + QP P P +P P P +
Sbjct: 43 QPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEP------PKEAPVVIEKPKPKP 96

Query: 85 QPMVSKMLGADGVTAGSVLLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSA-QQL 143
+P + V V+S + A T + A + ++ S + L
Sbjct: 97 KPKPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRAL 156

Query: 144 SMAK 147
S +
Sbjct: 157 SRNQ 160



Score = 31.5 bits (71), Expect = 0.002
Identities = 15/39 (38%), Positives = 18/39 (46%)

Query: 23 QREPAPVEEVKPAPEQPAEPQQPVPTVPSVPTIPQQPGP 61
Q P PV E +P PE EP + P V P +P P
Sbjct: 62 QPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKP 100


21B7485_06395B7485_06470Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_06395235-3.384675lysogenization protein HflD
B7485_06400138-5.006855tRNA 2-thiouridine(34) synthase MnmA
B7485_06405237-5.210789NUDIX hydrolase
B7485_06410124-1.68128423S rRNA pseudouridine synthase E
B7485_06415125-2.047983isocitrate dehydrogenase (NADP(+))
B7485_06420026-2.398948cyclic diguanylate phosphodiesterase
B7485_06425-127-2.509604hypothetical protein
B7485_06430-123-1.733648hypothetical protein
B7485_06435023-1.602421autotransporter outer membrane beta-barrel
B7485_06440226-1.857323hypothetical protein
B7485_064500231.421708glycine zipper family protein
B7485_064550232.153569hypothetical protein
B7485_064602233.087169hypothetical protein
B7485_064700263.253714autotransporter outer membrane beta-barrel
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06575PRTACTNFAMLY962e-22 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 96.3 bits (239), Expect = 2e-22
Identities = 142/655 (21%), Positives = 226/655 (34%), Gaps = 93/655 (14%)

Query: 137 DVDITTHGDNAHAIAARQGTVSFNQGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSG 196
D + + +V Q + AAI + G VT+ S A G+
Sbjct: 285 PGGFGPVLDGWYGVDVSGSSVELAQSIVEAPELGAAIR-VGRGARVTVSGGSLSAPHGNV 343

Query: 197 IVLESSIN--GQEATVDILSGSSLRSANEILYHKNETSNVTITDSEVSSAADVFINNIKG 254
I + Q A + I + + + L ++ V +T ++ AD + +
Sbjct: 344 IETGGARRFAPQAAPLSITLQAGAHAQGKALLYRVLPEPVKLT---LTGGADAQGDIVAT 400

Query: 255 HLTVDATNSKITGSANISTDDN------THTYLSLS-DNSTWDIKADSTVSNLTV--DNS 305
L S G +++ T SLS DN+TW + +S V L + D S
Sbjct: 401 ELPSIPGTS--IGPLDVALASQARWTGATRAVDSLSIDNATWVMTDNSNVGALRLASDGS 458

Query: 306 TVYISRADGRDVEPTRLTITENYVGNNGVLHLRTELDDDNSATDKVVINGNTSGTTRVKV 365
+ A+ + +T N + +G+ + D +DK+V+ + SG R+ V
Sbjct: 459 VDFQQPAEAGRFK----VLTVNTLAGSGLFRMNVFAD--LGLSDKLVVMQDASGQHRLWV 512

Query: 366 TNAGGSGAYTLNGIEIISVEGESNGEFI---KDSRIFAGAYEYSLTRGNTEATNKNWYLT 422
N+G S + N + ++ S F KD ++ G Y Y L N W L
Sbjct: 513 RNSG-SEPASANTLLLVQTPLGSAATFTLANKDGKVDIGTYRYRLA----ANGNGQWSLV 567

Query: 423 NFQAT-------SGGETNSGGSSAPTVAPTPVLRPEAGSYVANLAAANTLFVMRLNDRAG 475
+A G AP P A AA NT V A
Sbjct: 568 GAKAPPAPKPAPQPGPQPPQPPQPQPEAPAPQPPAGRELSAAANAAVNTGGV----GLAS 623

Query: 476 ETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRY-------VS--QLGGDLL 526
Y + +R L L G AW Q + +R V+ +LG D
Sbjct: 624 TLWYAESNALSKRLGELRLNPDAG-GAWGRGFAQRQQLDNRAGRRFDQKVAGFELGADHA 682

Query: 527 TGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAY 586
W LG +AGY R + G G YAT+ AD G Y
Sbjct: 683 VAVAGGR--WHLGGLAGYTR----GDRGFTGDGG-GHTDSVHVGGYATYIADS----GFY 731

Query: 587 IDSWAQYSWFKN----------SVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAA 636
+D+ + S +N +VKG Y G SLEAG F
Sbjct: 732 LDATLRASRLENDFKVAGSDGYAVKGK------YRTHGVGASLEAGRRFTHADG------ 779

Query: 637 KYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGD 696
W +PQA+ A+ ANG R+ ++ +++ RLG R + G
Sbjct: 780 ---WFLEPQAELAVFRAGGGAYRAANGLRVRDEGGSSVLGRLGLEVGKRIELAG----GR 832

Query: 697 DFEPFVEMNWIHNSK-DFAVSMNGVKVEQDGVSNLGEIKLGVNGNLNPAASVWGN 750
+P+++ + + V NG+ + E+ LG+ L S++ +
Sbjct: 833 QVQPYIKASVLQEFDGAGTVHTNGIAHRTELRGTRAELGLGMAAALGRGHSLYAS 887


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06600PRTACTNFAMLY507e-10 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 49.7 bits (118), Expect = 7e-10
Identities = 30/114 (26%), Positives = 50/114 (43%), Gaps = 1/114 (0%)

Query: 34 YHLSNGMESKSVDTRSIYRELGATLSYNMRLGNGMEIEPCLKAAVRKEFVDDNRVKVNSD 93
Y +NG+ + S+ LG + + L G +++P +KA+V +EF V N
Sbjct: 798 YRAANGLRVRDEGGSSVLGRLGLEVGKRIELAGGRQVQPYIKASVLQEFDGAGTVHTNGI 857

Query: 94 GNFVNDLSGRRGIYQAGIKASFSSTLSGHFGVGYSHGAGVESPWNAVAGVNWSF 147
+ +L G R G+ A+ S + YS G + PW AG +S+
Sbjct: 858 AH-RTELRGTRAELGLGMAAALGRGHSLYASYEYSKGPKLAMPWTFHAGYRYSW 910


22B7485_06535B7485_06665Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_06535-115-3.224462isomerase/hydrolase
B7485_06545116-4.505757hypothetical protein
B7485_06550124-5.277970protein UmuD
B7485_06555125-6.326365DNA polymerase V subunit UmuC
B7485_06560125-5.482491disulfide bond formation protein B
B7485_06570127-6.019281Na+/H+ antiporter NhaB
B7485_06575125-5.101812fatty acid metabolism transcriptional regulator
B7485_06580228-6.487549SpoVR family protein
B7485_06585029-6.703235D-amino acid dehydrogenase small subunit
B7485_06590125-6.125810alanine racemase
B7485_06595-123-4.019269invasion protein
B7485_06605024-4.634992K+/H+ antiporter
B7485_06615-219-2.037579muramoyltetrapeptide carboxypeptidase
B7485_06620-225-2.833324hypothetical protein
B7485_06625-127-1.346877murein transglycosylase
B7485_06630127-0.729618flagellar brake protein
B7485_06635126-1.183508GlsB/YeaQ/YmgE family stress response membrane
B7485_06640-124-2.298020molybdenum transporter ModD
B7485_06645-119-1.933028SAM-dependent methyltransferase
B7485_06650-122-3.337871ABC transporter ATP-binding protein
B7485_06655-222-5.587401iron ABC transporter permease
B7485_06660-221-4.221417iron ABC transporter substrate-binding protein
B7485_06665-219-3.320200hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06715ALARACEMASE5540.0 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 554 bits (1429), Expect = 0.0
Identities = 353/356 (99%), Positives = 354/356 (99%)

Query: 1 MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN 60
MTRPIQASLDLQALKQNLSIVRQAA HARVWSVVKANAYGHGIERIWSA+GATDGFALLN
Sbjct: 1 MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLN 60

Query: 61 LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI 120
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
Sbjct: 61 LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI 120

Query: 121 YLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA 180
YLKVNSGMNRLGFQ DRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA
Sbjct: 121 YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA 180

Query: 181 EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII 240
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
Sbjct: 181 EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII 240

Query: 241 GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS 300
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
Sbjct: 241 GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS 300

Query: 301 MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV 356
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
Sbjct: 301 MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV 356


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06730BCTERIALGSPH300.011 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 29.5 bits (66), Expect = 0.011
Identities = 13/41 (31%), Positives = 18/41 (43%), Gaps = 1/41 (2%)

Query: 168 TEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFR 208
T G++ GG L L G W P +L+ + PFR
Sbjct: 106 TSGSIAGGKL-NLAFAQGEAWTPGDNPDVLIFPGGEMTPFR 145


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06770LCRVANTIGEN300.011 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 29.7 bits (66), Expect = 0.011
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 7/63 (11%)

Query: 193 LMSTHHPLHANAIADSIIQVEPDGRVTQGLPTEQLTTNKLAAL------YRVSADQIHHH 246
+ H L A+ I D I++V D G +L +LA L Y V +I+ H
Sbjct: 119 MAVMHFSLTADRIDDDILKVIVDSMNHHGDARSKL-REELAELTAELKIYSVIQAEINKH 177

Query: 247 LSA 249
LS+
Sbjct: 178 LSS 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06780FERRIBNDNGPP401e-05 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 39.5 bits (92), Expect = 1e-05
Identities = 65/301 (21%), Positives = 102/301 (33%), Gaps = 48/301 (15%)

Query: 2 PITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNI--TRIVSAG-APADLLL-L 57
I+RR A+A + L + A I RIV+ P +LLL L
Sbjct: 6 LISRRRLLTAMALSPL-------------LWQMNTAHAAAIDPNRIVALEWLPVELLLAL 52

Query: 58 AVAPEKMVGFSSFDFARQALI--PLPEHIRQLPRLGRLAGRASTLSLEGLMALHPDLVVD 115
+ P G + R + PLP+ + + G + +LE L + P +V
Sbjct: 53 GIVP---YGVADTINYRLWVSEPPLPDSVIDV-------GLRTEPNLELLTEMKPSFMVW 102

Query: 116 CGNTDETLISQARQVSEQTQIPWLLLN-----GKLAQSAEQLTTLGKTLGEEHRAAEQAN 170
+ AR P N LA + + LT + L + A
Sbjct: 103 SAGYGPSPEMLARIA------PGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLA 156

Query: 171 LASHFVGEAQA-FATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLGLHNVAQ-IADR 228
F+ + F A L PR + SL E + G+ N Q +
Sbjct: 157 QYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNF 216

Query: 229 HGLTQVSMENLLRWQ-PDIILVQEAVTADF--IRRDPLWQGVKAVAEQRILFLSGLPFGW 285
G T VS++ L ++ D++ + D + PLWQ + V R +P W
Sbjct: 217 WGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGR---FQRVPAVW 273

Query: 286 L 286

Sbjct: 274 F 274


23B7485_06990B7485_07120Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_06990-222-3.857255hypothetical protein
B7485_06995-126-4.508382voltage-gated potassium channel
B7485_07000-120-2.705637IS3 family transposase
B7485_07005024-2.288555hypothetical protein
B7485_07010025-2.255008TonB system transport protein TonB
B7485_07015025-2.652607acyl-CoA thioesterase
B7485_07020021-2.800049intracellular septation protein A
B7485_07025020-2.526425hypothetical protein
B7485_07030-123-2.950691outer membrane protein W
B7485_07035014-3.340355YciE/YciF family protein
B7485_07040-112-2.717321YciE/YciF family protein
B7485_07050014-0.984446hypothetical protein
B7485_07060016-2.800236tryptophan synthase subunit alpha
B7485_07065-119-3.056422tryptophan synthase subunit beta
B7485_07070-221-3.702223bifunctional indole-3-glycerol phosphate
B7485_07075-222-2.749293bifunctional glutamine
B7485_07080-225-3.751269anthranilate synthase component I
B7485_07085-123-4.015893trp operon leader peptide
B7485_07090018-1.891076phosphatase
B7485_07095216-2.016032threonylcarbamoyl-AMP synthase
B7485_07100216-1.83210023S rRNA pseudouridylate synthase B
B7485_07110122-5.623286cob(I)yrinic acid a,c-diamide
B7485_07115027-5.313766NAD(P)-dependent oxidoreductase
B7485_07120-122-3.892560protease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07095adhesinmafb314e-04 Neisseria meningitidis: adhesin MafB signature.
		>adhesinmafb#Neisseria meningitidis: adhesin MafB signature.

Length = 467

Score = 31.2 bits (70), Expect = 4e-04
Identities = 16/57 (28%), Positives = 20/57 (35%), Gaps = 2/57 (3%)

Query: 41 GPMPAVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKV 97
P+PA G GS E + EA W +P A V +V KV
Sbjct: 268 APLPA--EGKFAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAVFNVAAAAKV 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07100TONBPROTEIN2494e-86 Gram-negative bacterial tonB protein signature.
		>TONBPROTEIN#Gram-negative bacterial tonB protein signature.

Length = 239

Score = 249 bits (638), Expect = 4e-86
Identities = 233/239 (97%), Positives = 233/239 (97%), Gaps = 4/239 (1%)

Query: 1 MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVAPADLEPPQA 60
MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMV PADLEPPQA
Sbjct: 1 MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVTPADLEPPQA 60

Query: 61 VQPPPEPVVEPEPEPEPIPEPPKEAPVVIE----KPKPKPKPVKKVQEQPKRDVKPVESR 116
VQPPPEPVVEPEPEPEPIPEPPKEAPVVIE KPKPKPKPVKKVQEQPKRDVKPVESR
Sbjct: 61 VQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESR 120

Query: 117 PASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKF 176
PASPFENTAPAR TSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKF
Sbjct: 121 PASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKF 180

Query: 177 DVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ 235
DVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ
Sbjct: 181 DVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ 239


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07195DHBDHDRGNASE871e-22 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 87.0 bits (215), Expect = 1e-22
Identities = 62/239 (25%), Positives = 105/239 (43%), Gaps = 23/239 (9%)

Query: 13 RIILVTGASDGIGREAAMTYARYGATVILLGRNEEKLRQVASHINEETGRQPQWFILDLL 72
+I +TGA+ GIG A T A GA + + N EKL +V S + E R + F D+
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE-ARHAEAFPADV- 66

Query: 73 TCTSENCQQLAQRIVVNYPRLDGVLHNAGLLGDVCPMSEQNPQVWQDVMQVNVNATFMLT 132
S ++ RI +D +++ AG+L + + + W+ VN F +
Sbjct: 67 -RDSAAIDEITARIEREMGPIDILVNVAGVL-RPGLIHSLSDEEWEATFSVNSTGVFNAS 124

Query: 133 QALLPLLLKSDAGSLVFTSSSVGRQGRANWGAYAASKFATEGMMQVLADEYQQR-LRVNC 191
+++ ++ +GS+V S+ R + AYA+SK A + L E + +R N
Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 192 INPGGTRTAMRASAFPTEDPQ------------------KLKTPADIMPLYLWLMGDDS 232
++PG T T M+ S + E+ KL P+DI L+L+ +
Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQA 243


24B7485_07370B7485_07420Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_07370-1173.637460transient receptor potential locus
B7485_073801193.344182family 65 glycosyl hydrolase
B7485_073852203.422568beta-phosphoglucomutase
B7485_073953161.237585hypothetical protein
B7485_07400213-0.943518hypothetical protein
B7485_07405015-3.813585LacI family transcriptional regulator
B7485_07410016-2.972971hypothetical protein
B7485_07415019-4.209507TIGR01620 family protein
B7485_07420-114-3.382698TyrR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07500OUTRMMBRANEA260.005 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 26.4 bits (58), Expect = 0.005
Identities = 9/43 (20%), Positives = 16/43 (37%)

Query: 12 GLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYS 54
G F GY + + Y + + +G + Y GV +
Sbjct: 59 GAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLT 101


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07520HTHFIS316e-104 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 316 bits (810), Expect = e-104
Identities = 122/422 (28%), Positives = 188/422 (44%), Gaps = 44/422 (10%)

Query: 130 NGFNFLRWLESEPQDSHNEHVVINGQNFLMEITPVYLQDENDQH----VLTGAVVMLRST 185
N F+ L ++ +V++ QN M + D LT + ++
Sbjct: 61 NAFDLLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118

Query: 186 IRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVEQAQKLAMLSAPLLITGDTGTGKDLFAY 245
+ ++ + D +V S M+ + +L L+ITG++GTGK+L A
Sbjct: 119 LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVAR 178

Query: 246 ACHQASPRAGKPYLALNCASIPEDAVESELFGH-------APEGKKGFFEQANGGSVLLD 298
A H R P++A+N A+IP D +ESELFGH A G FEQA GG++ LD
Sbjct: 179 ALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLD 238

Query: 299 EIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVRVICATQKNLVELVQKGVFREDLYY 358
EIG+M Q +LLR L G + VG + DVR++ AT K+L + + +G+FREDLYY
Sbjct: 239 EIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYY 298

Query: 359 RLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQGVPRPKLAADLNTVLTRYAWPGNVRQ 418
RLNV+ L LPPLRD +DI L FV + E G+ + + ++ + WPGNVR+
Sbjct: 299 RLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKE-GLDVKRFDQEALELMKAHPWPGNVRE 357

Query: 419 LKNAIYRALTQLDGYELRPQDILLPDYDAATVAVGEDAM--------------------- 457
L+N + R + + I + E A
Sbjct: 358 LENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFA 417

Query: 458 --------EGSLDEITSRFERSVLTQ-LYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ 508
G D + + E ++ L + K A LG++ + K+RE G+S
Sbjct: 418 SFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSV 477

Query: 509 KK 510
+
Sbjct: 478 YR 479


25B7485_07545B7485_07845Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_075452232.405356hypothetical protein
B7485_075503252.551719hypothetical protein
B7485_075551260.121908IS3 family transposase
B7485_07560025-1.435352hypothetical protein
B7485_07565-121-2.879963****integrase
B7485_07570025-4.449194hypothetical protein
B7485_07575028-4.947926hypothetical protein
B7485_07580026-4.136007phage tail protein
B7485_07585127-3.915632phage tail protein
B7485_07590126-2.372736hypothetical protein
B7485_07595330-2.582930metal ABC transporter permease
B7485_07600129-2.451212metal ABC transporter permease
B7485_07605028-2.518561manganese/iron transporter ATP-binding protein
B7485_07610027-2.760167metal ABC transporter substrate-binding protein
B7485_07620129-2.170897site-specific integrase
B7485_07625126-2.456765DNA-binding protein
B7485_07630629-2.730015IS3 family transposase
B7485_07635731-3.040105host-nuclease inhibitor protein Gam
B7485_07640630-2.377364IS3 family transposase
B7485_07645629-2.432714hypothetical protein
B7485_07650726-2.547347hypothetical protein
B7485_07680221-2.095470cell division protein FtsZ
B7485_07690320-1.273733IS3 family transposase
B7485_07695319-1.491234general secretion pathway protein GspL
B7485_07700317-1.071834transposase
B7485_07705316-0.445838hypothetical protein
B7485_07710319-1.031402hypothetical protein
B7485_07715118-1.873935E3 ubiquitin--protein ligase
B7485_07720017-1.727299serine/threonine protein phosphatase
B7485_07730121-2.673666IS3 family transposase
B7485_07735124-1.841021hypothetical protein
B7485_07740025-2.318452hypothetical protein
B7485_07745125-1.308396ribosomal RNA small subunit methyltransferase F
B7485_07750224-1.948417MCE family protein
B7485_07755226-1.621123Free methionine-R-sulfoxide reductase
B7485_07760124-1.465548RNA chaperone ProQ
B7485_07765124-1.791604tail-specific peptidase
B7485_07770223-1.563921protease HtpX
B7485_07775223-2.706024MFS transporter
B7485_07780122-3.031951transcriptional regulator KdgR
B7485_07785124-1.849383hypothetical protein
B7485_07790226-2.012694PhoP regulon feedback inhibition membrane
B7485_07800128-1.870720hypothetical protein
B7485_07805128-1.234473hypothetical protein
B7485_07810125-0.234571DUF2627 domain-containing protein
B7485_07815125-0.617420cold-shock protein CspC
B7485_07820725-1.46151523S rRNA (guanine(745)-N(1))-methyltransferase
B7485_07825724-1.772771hypothetical protein
B7485_07830525-1.821191hypothetical protein
B7485_07835524-1.718786PTS mannose transporter subunit IID
B7485_07840527-2.509726PTS mannose/fructose/sorbose transporter subunit
B7485_07845526-3.020802PTS mannose transporter subunit EIIAB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_0768056KDTSANTIGN260.013 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 25.7 bits (56), Expect = 0.013
Identities = 9/18 (50%), Positives = 9/18 (50%)

Query: 1 MSQQPQQPQQPQQPQQPQ 18
M Q QQ Q Q QQ Q
Sbjct: 336 MPPQAQQQQGQGQQQQAQ 353



Score = 24.1 bits (52), Expect = 0.047
Identities = 8/17 (47%), Positives = 8/17 (47%)

Query: 3 QQPQQPQQPQQPQQPQQ 19
P Q QQ Q Q QQ
Sbjct: 335 VMPPQAQQQQGQGQQQQ 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07735adhesinb331e-116 Adhesin B signature.
		>adhesinb#Adhesin B signature.

Length = 310

Score = 331 bits (849), Expect = e-116
Identities = 90/296 (30%), Positives = 163/296 (55%), Gaps = 7/296 (2%)

Query: 9 MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQP 68
+G A + + + + K V+ T +IIAD+ KN+AGD + SI G + HEY+P
Sbjct: 13 AFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSIVPVGQDPHEYEP 72

Query: 69 TPGDIKRAQGAQLILANGMNLEL----WFQRFYQHLNGVPE---VIVSSGVTPVGITEGP 121
P D+K+ A LI NG+NLE WF + ++ VS GV + +
Sbjct: 73 LPEDVKKTSQADLIFYNGINLETGGNAWFTKLVENAKKKENKDYYAVSEGVDVIYLEGQS 132

Query: 122 YEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKITQTLAPLRKQI 181
+GK +PHAW++ +N +IY NI L + DPAN +TY++N Y K++ +++
Sbjct: 133 EKGKEDPHAWLNLENGIIYAQNIAKRLSEKDPANKETYEKNLKAYVEKLSALDKEAKEKF 192

Query: 182 TELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAV 241
+P ++ +VTSEG F Y ++ + Y+W IN +++GTP Q++ +V+ ++K +P++
Sbjct: 193 NNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTLVEKLRKTKVPSL 252

Query: 242 FSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGI 297
F ES++ D+P + V+++T ++ DS++ + +Y ++K + +G+
Sbjct: 253 FVESSVDDRPMKTVSKDTNIPIYAKIFTDSVAEKGEEGDSYYSMMKYNLEKIAEGL 308


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_07915TCRTETB1132e-29 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 113 bits (283), Expect = 2e-29
Identities = 84/397 (21%), Positives = 171/397 (43%), Gaps = 14/397 (3%)

Query: 27 MAVLDGAIANVALPTIATDLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKC 86
+VL+ + NV+LP IA D + PAS+ WV A+ + I + L D G +R+
Sbjct: 25 FSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLF 84

Query: 87 GLVVFLLSSLFCALSDS-LQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGIN 145
G+++ S+ + S +L +AR IQG G AA ++ ++ P+ G+ G+
Sbjct: 85 GIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLI 144

Query: 146 SFIVAVSSAAGPTIAAAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDL 205
IVA+ GP I I W +L LI + + II + M+ L K FD+
Sbjct: 145 GSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM-ITIITVPFLMKLLKKEVRI--KGHFDI 201

Query: 206 PSAVMNALTFGLLITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLL 265
++ + I F S++ L+V V+ + F++ + P + L
Sbjct: 202 KGIIL----MSVGIVFFMLFTTSYSISF----LIVSVLSFLIFVKHIRKVTDPFVDPGLG 253

Query: 266 RIPLFSLSICTSVCSFCAQMLAMVSLPFYLQTVLGRSEVETG-LLLTPWPLATMVMAPLA 324
+ F + + F + +P+ ++ V S E G +++ P ++ ++ +
Sbjct: 254 KNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIG 313

Query: 325 GYLIERVHAGLLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHT 384
G L++R + +G+ ++ + L + + + ++ G ++ +
Sbjct: 314 GILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWF-MTIIIVFVLGGLSFTKTVISTI 372

Query: 385 IITSAPRERSGGASGMLGTARLLGQSSGAALVALMLN 421
+ +S ++ +G +L L + +G A+V +L+
Sbjct: 373 VSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409


26B7485_07915B7485_07960Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_07915-218-3.097258long-chain-fatty-acid--CoA ligase
B7485_07920021-6.290407ribonuclease D
B7485_07925226-8.653253ferredoxin--NADP(+) reductase
B7485_07930022-4.711863ring-hydroxylating oxygenase subunit alpha
B7485_07935-221-3.378996transcriptional regulator
B7485_07940-120-2.626311hypothetical protein
B7485_07945018-0.811038IS3 family transposase
B7485_07950019-1.326657PrkA family serine protein kinase
B7485_07955017-0.571979MltA-interacting protein
B7485_07960222-0.609573aldo/keto reductase
27B7485_08075B7485_08155Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_08075318-2.312814NTP pyrophosphohydrolase
B7485_08080220-2.436721hypothetical protein
B7485_08085115-1.283150ABC transporter ATP-binding protein
B7485_08090218-1.251711IS4 family transposase
B7485_08095120-1.356376ABC transporter substrate-binding protein
B7485_08100024-1.665146carboxymuconolactone decarboxylase family
B7485_08105021-2.083689TVP38/TMEM64 family protein
B7485_08110-118-2.387114hypothetical protein
B7485_08115-117-3.149837exodeoxyribonuclease III
B7485_08125123-4.596309succinyldiaminopimelate aminotransferase
B7485_08130122-4.455975succinylornithine aminotransferase
B7485_08135024-5.522113arginine N-succinyltransferase
B7485_08140124-6.386896N-succinylglutamate 5-semialdehyde
B7485_08145021-5.708844succinylglutamate desuccinylase
B7485_08150118-4.639166ATP-independent periplasmic protein-refolding
B7485_08155-113-3.001863protein Ves
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08310DNABINDINGHU310.002 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 31.2 bits (71), Expect = 0.002
Identities = 14/61 (22%), Positives = 28/61 (45%), Gaps = 5/61 (8%)

Query: 74 SNKAELTAIIARETGKPRWEAATEVTAMINKIAISIKAYHVRTGEQRSEMPDGAASLRHR 133
+NK +L A +A T + ++A V A+ + ++ + GE+ + G +R R
Sbjct: 2 ANKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAK-----GEKVQLIGFGNFEVRER 56

Query: 134 P 134

Sbjct: 57 A 57


28B7485_08225B7485_08475Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_082252132.256831metal-dependent hydrolase
B7485_082302152.6772692-deoxyglucose-6-phosphate phosphatase
B7485_082351142.556587hypothetical protein
B7485_082401142.577504fructosamine kinase family protein
B7485_082451163.126059hypothetical protein
B7485_082501163.0314576-phosphofructokinase II
B7485_082550181.406021hypothetical protein
B7485_082600180.218841hypothetical protein
B7485_08265117-0.054941threonine--tRNA ligase
B7485_082700150.296205translation initiation factor IF-3
B7485_08275-1140.14547650S ribosomal protein L35
B7485_082800161.22991350S ribosomal protein L20
B7485_082851133.180867phenylalanine--tRNA ligase subunit alpha
B7485_082900144.015094phenylalanine--tRNA ligase subunit beta
B7485_082950143.974926integration host factor subunit alpha
B7485_083000143.652505vitamin B12 import system permease BtuC
B7485_083051133.345384glutathione peroxidase
B7485_083101142.526539vitamin B12 import ATP-binding protein BtuD
B7485_083151120.492807lipoprotein
B7485_08320013-0.698727EAL domain-containing protein
B7485_08325016-1.977669hypothetical protein
B7485_08330119-2.749318hemin uptake protein HemP
B7485_08335218-2.991421phospho-2-dehydro-3-deoxyheptonate aldolase
B7485_08340117-3.626136phosphoenolpyruvate synthase regulatory protein
B7485_08345119-4.181001hypothetical protein
B7485_08355219-5.412216phosphoenolpyruvate synthase
B7485_08365-114-3.301465cyclohexanecarboxylate-CoA ligase
B7485_08375014-4.692894ferredoxin family protein
B7485_08380-316-3.058463oxidoreductase
B7485_08385-217-2.294120electron transfer flavoprotein
B7485_08390-119-2.184536hypothetical protein
B7485_08395-127-3.902469cupin domain-containing protein
B7485_08405023-1.362261sulfatase
B7485_08415-120-0.896888transcriptional regulator
B7485_08420-120-1.235770Two-protein-system connector protein SafA
B7485_08425021-0.333090oxidoreductase
B7485_08435121-2.178936hypothetical protein
B7485_08440325-4.927910antitoxin RelB
B7485_08445015-4.617263mRNA interferase RelE
B7485_08450016-4.898837protein HokD
B7485_08455016-4.835193hypothetical protein
B7485_08460120-3.030256hypothetical protein
B7485_08465223-2.494652hypothetical protein
B7485_08470327-0.041962antitermination protein
B7485_084754270.631817**hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08500DNABINDINGHU1193e-39 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 119 bits (301), Expect = 3e-39
Identities = 34/89 (38%), Positives = 55/89 (61%)

Query: 4 TKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQRPGR 63
K ++ + + L+K+D+ V+ F + L GE+V+L GFGNF++R++ R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 64 NPKTGEDIPITARRVVTFRPGQKLKSRVE 92
NP+TGE+I I A +V F+ G+ LK V+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAVK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08515PF05272300.008 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.008
Identities = 9/22 (40%), Positives = 13/22 (59%)

Query: 28 ILHLVGPNGAGKSTLLAQMAGM 49
+ L G G GKSTL+ + G+
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08555PHPHTRNFRASE2973e-93 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 297 bits (761), Expect = 3e-93
Identities = 116/453 (25%), Positives = 200/453 (44%), Gaps = 73/453 (16%)

Query: 363 RAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKK-ASAIVTNRGGRTCH 421
R +GH IG ++ + E ++ D+T D + K+ T+ GGRT H
Sbjct: 137 RVLGHLIGVE----TGSLATIA--EETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSH 190

Query: 422 AAIIARELGIPAVVGCGDATERMKDGENVTVSCAEG---------DTGYVYAELLEFSVK 472
+AI++R L IPAVVG + TE+++ G+ V V EG + + F +
Sbjct: 191 SAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYEEKRAAFEKQ 250

Query: 473 SSSVETMPDLP--------LKVMMNVGNPDRAFDFACLPSEGVGLARLEFII-NRMIGVH 523
+ P +++ N+G P EG+GL R EF+ +R
Sbjct: 251 KQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFLYMDRD---- 306

Query: 524 PRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFK 583
+ +E Q + +E+++ K V++R D
Sbjct: 307 -----QLPTEEEQFE-AYKEVVQ----------------------RMDGKPVVIRTLDIG 338

Query: 584 SNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEI 643
++ + + P E NP LGFR + +D F + A+ R N+++
Sbjct: 339 GDKELSYL----QLPKELNPFLGFRAIRLCL--EKQDIFRTQLRALLRAS---TYGNLKV 389

Query: 644 MIPFVRTVD---QAKAVVEELARQGLKRG---ENGLKIIMMCEIPSNALLAEQFLEYFDG 697
M P + T++ QAKA+++E + L G + +++ +M EIPS A+ A F + D
Sbjct: 390 MFPMIATLEELRQAKAIMQEEKDKLLSEGVDVSDSIEVGIMVEIPSTAVAANLFAKEVDF 449

Query: 698 FSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSD 757
FSIG+ND+ Q + DR + VS L+ + A+ L+ M I+AA +GK+VG+CG+ D
Sbjct: 450 FSIGTNDLIQYTMAADRMNERVSYLYQPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGD 509

Query: 758 HEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAEL 790
L+ G+D S++ +++ L +L
Sbjct: 510 E-VAIPLLLGLGLDEFSMSATSILPARSQLLKL 541


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08650HOKGEFTOXIC615e-17 Hok/Gef cell toxic protein family signature.
		>HOKGEFTOXIC#Hok/Gef cell toxic protein family signature.

Length = 52

Score = 60.6 bits (147), Expect = 5e-17
Identities = 19/46 (41%), Positives = 32/46 (69%)

Query: 4 QKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEP 49
+ +++ ++++CLT+++ +TRK LCE+R R G EVA F AYE
Sbjct: 5 RSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYES 50


29B7485_08580B7485_08700Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_08580122-4.974911transcriptional regulator
B7485_08585127-7.298057stress protection protein MarC
B7485_08590023-4.303843sugar efflux transporter
B7485_08595124-4.343542hypothetical protein
B7485_08600124-4.365563aldehyde dehydrogenase
B7485_08605021-3.627924glutaminase 2
B7485_08610222-2.571525DUF4186 domain-containing protein
B7485_08615222-2.110479GGDEF domain-containing protein
B7485_08620528-2.891598diguanylate cyclase
B7485_08630531-4.322444altronate oxidoreductase
B7485_08640123-1.380623IS91 family transposase
B7485_08645-124-0.750511hypothetical protein
B7485_08650-121-0.908417hypothetical protein
B7485_08655-123-1.881034hypothetical protein
B7485_08665124-3.010117trans-aconitate 2-methyltransferase
B7485_08670332-5.061890autoinducer 2-degrading protein LsrG
B7485_08675535-5.9850713-hydroxy-5-phosphonooxypentane-2,4-dione
B7485_086901037-6.689414autoinducer 2-binding protein lsrB
B7485_08695429-3.794670hypothetical protein
B7485_08700221-3.869694histidine kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08810TCRTETB537e-10 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 53.3 bits (128), Expect = 7e-10
Identities = 40/192 (20%), Positives = 83/192 (43%), Gaps = 8/192 (4%)

Query: 36 LSDIAQSFHMQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLS 95
L DIA F+ A + T + ++ + + ++ Q+ ++LL+ ++ V+
Sbjct: 37 LPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIG 96

Query: 96 FLSWS-FTVLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGL 154
F+ S F++L+++R A F ++ + R P R +A LI + A+ +G
Sbjct: 97 FVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGP 156

Query: 155 PLGRIVGQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSI 214
+G ++ Y W I + ++T+ L+KLL + LMS+
Sbjct: 157 AIGGMIAHYIHWSY-LLLIPMITIITVPFLMKLLK------KEVRIKGHFDIKGIILMSV 209

Query: 215 YLLTVVVVTAHY 226
++ ++ T Y
Sbjct: 210 GIVFFMLFTTSY 221


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_08825BLACTAMASEA290.015 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 29.4 bits (66), Expect = 0.015
Identities = 12/51 (23%), Positives = 21/51 (41%), Gaps = 1/51 (1%)

Query: 22 GQGKVADYIPALATVDGSRLGI-AICTVDGQLFQAGDAQERFSIQSISKVL 71
+ + I + R+G+ + G+ A A ERF + S KV+
Sbjct: 21 ASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTFKVV 71


30B7485_08945B7485_09010Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_08945-218-3.761425arginine:ornithine antiporter
B7485_08950-227-4.149321dihydrofolate reductase FolM
B7485_08955-220-2.608917hypothetical protein
B7485_08960-122-2.433724DNA-binding response regulator
B7485_08965123-2.175049L-cystine transporter
B7485_08970220-1.630203two-component sensor histidine kinase
B7485_089753220.008491DNA replication terminus site-binding protein
B7485_08980419-0.367343class II fumarate hydratase
B7485_089905240.307231fumarate hydratase
B7485_09000522-0.391784mannose-6-phosphate isomerase
B7485_09005121-0.188590DUF945 domain-containing protein
B7485_09010221-0.314335glucuronide uptake porin UidC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_09145DHBDHDRGNASE290.016 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 28.9 bits (64), Expect = 0.016
Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 1/32 (3%)

Query: 151 AYAASKAALDNMTRSFARKLAPE-VKVNSIAP 181
AYA+SKAA T+ +LA ++ N ++P
Sbjct: 156 AYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_09155HTHFIS661e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 1e-14
Identities = 27/131 (20%), Positives = 60/131 (45%), Gaps = 3/131 (2%)

Query: 3 TIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMT 62
TI+ +DDA + +++ L++ V + I + DLV+ D+++P ++
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 63 ICRDLRAKWSG-PIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLR--LHLRQN 119
+ ++ P++++++ ++ M I A E GA DY+ K L+ + L +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 120 EQATLTKGLQE 130
+ L Q+
Sbjct: 125 RPSKLEDDSQD 135


31B7485_09620B7485_09705Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_09620319-4.020722nitrate reductase subunit beta
B7485_09625216-3.558212transposase
B7485_09635117-4.387760colanic acid/biofilm transcriptional regulator
B7485_09640117-4.354647NADP-dependent oxidoreductase
B7485_09645-116-4.653183IS3 family transposase
B7485_09650-119-6.187355N-acetyltransferase
B7485_09655-121-7.221537hypothetical protein
B7485_09660121-7.524043hypothetical protein
B7485_09665-121-7.430818hypothetical protein
B7485_09670-121-7.714358hypothetical protein
B7485_09675-115-6.275406stress response membrane protein YncL
B7485_09680-113-5.003057acetyltransferase
B7485_09685-113-3.647276gamma-aminobutyraldehyde dehydrogenase
B7485_09690-314-3.219290ABC transporter permease
B7485_09695014-2.566537ABC transporter permease
B7485_09705013-3.051539hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_09875SACTRNSFRASE362e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.1 bits (83), Expect = 2e-05
Identities = 13/61 (21%), Positives = 30/61 (49%), Gaps = 1/61 (1%)

Query: 78 RHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFV 137
+E + V D++ KG+G LL + I+ A++ ++ + N ++ H + F+
Sbjct: 89 YALIED-IAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI 147

Query: 138 V 138
+
Sbjct: 148 I 148


32B7485_09880B7485_10030Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_09880330-9.689595hypothetical protein
B7485_09885023-6.045803IS3 family transposase
B7485_09890022-4.633944heat-shock protein
B7485_09895020-3.5593832-hydroxyacid dehydrogenase
B7485_09900-119-2.580657hypothetical protein
B7485_09905018-1.920338lipoprotein
B7485_099102212.703471hypothetical protein
B7485_099203240.480033AraC family transcriptional regulator
B7485_09925421-0.792161DUF333 domain-containing protein
B7485_09935320-2.109784pyruvate:ferredoxin (flavodoxin) oxidoreductase
B7485_09940123-4.168872IS3 family transposase
B7485_09945121-3.124552universal stress protein F
B7485_09950-119-2.879354tRNA 2-thiocytidine(32) synthetase TtcA
B7485_09955-218-3.211064ATP-dependent RNA helicase
B7485_09960-218-4.053082hypothetical protein
B7485_09965-118-3.596280zinc transporter ZntB
B7485_09970-214-2.360839DNA endonuclease SmrA
B7485_09975116-3.915801HlyD family secretion protein
B7485_09980-115-2.568541hypothetical protein
B7485_09985-116-1.767089HlyD family secretion protein
B7485_09990-316-1.205110AraC family transcriptional regulator
B7485_09995-217-2.078581pump protein
B7485_10005121-1.235865methylated-DNA--protein-cysteine
B7485_10010223-1.965221transcriptional regulator FNR
B7485_10015223-1.898562universal stress protein E
B7485_10020330-2.528912transposase
B7485_10025125-1.169366IS91 family transposase
B7485_10030019-3.434241IS3 family transposase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10145PF06291322e-04 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 31.9 bits (72), Expect = 2e-04
Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 6/51 (11%)

Query: 1 MKKVAAFVALSLLMAGC------VSNDKIAVTPEQLQHHRFVLESVNGKPV 45
MKK+ AL++L+ GC V N AVTP++ H F + + K
Sbjct: 6 MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVSGIGQKKT 56


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10215PRTACTNFAMLY311e-04 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 30.8 bits (69), Expect = 1e-04
Identities = 12/31 (38%), Positives = 12/31 (38%)

Query: 10 PVPEPIPGDPVPVPDPIPRPQPMPDPPPDEE 40
P P P PG P P P P PP E
Sbjct: 575 PKPAPQPGPQPPQPPQPQPEAPAPQPPAGRE 605



Score = 26.6 bits (58), Expect = 0.005
Identities = 11/23 (47%), Positives = 11/23 (47%)

Query: 19 PVPVPDPIPRPQPMPDPPPDEEP 41
P P P P P PQP P P E
Sbjct: 573 PAPKPAPQPGPQPPQPPQPQPEA 595


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10240RTXTOXIND736e-18 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 72.9 bits (179), Expect = 6e-18
Identities = 28/137 (20%), Positives = 54/137 (39%), Gaps = 14/137 (10%)

Query: 16 RQAAVVRAPIDGIVANRSAHT-GSWVEGGTSLVSLVPVSE-LWVDANYKENQIAGMKPGM 73
+QA+V+RAP+ V HT G V +L+ +VP + L V A + I + G
Sbjct: 325 QQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQ 384

Query: 74 KAEIRADILKGEVFH---GHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDA 130
A I+ + + G +++++ + G ++ +
Sbjct: 385 NAIIKVEAFPYTRYGYLVGKVKNINLDA-------IEDQRLGLVFNVIISIEENCLSTGN 437

Query: 131 KELKQLLRPGLSVTVSV 147
K + L G++VT +
Sbjct: 438 KNIP--LSSGMAVTAEI 452


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10265RTXTOXIND642e-14 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 64.5 bits (157), Expect = 2e-14
Identities = 40/212 (18%), Positives = 79/212 (37%), Gaps = 16/212 (7%)

Query: 11 VVAIGILLTGVVFFIW----RVSKGRFIQTTDDAYIGGNITTVASKVSGYISAIEVRDNQ 66
+VA I+ V+ FI +V G + + + I V++ +
Sbjct: 59 LVAYFIMGFLVIAFILSVLGQVEIV--ATANGKLTHSGRSKEIKPIENSIVKEIIVKEGE 116

Query: 67 SVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEGIQATITMQQ-----SIIQSASETW 121
SV+KGD++L+L A+ + ++ + ++ Q + + +
Sbjct: 117 SVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYF 176

Query: 122 QAVKHEEQKRLRD--TERYEKLAQSAAISQQIIDNARFDYQQVAAKERKAANDFLVEKQR 179
Q V EE RL E++ + +D R + V A+ + N VEK R
Sbjct: 177 QNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSR 236

Query: 180 LAVLSAQEEN---VRASIEEVQAALTQALLDL 208
L S+ + ++ E + +A+ +L
Sbjct: 237 LDDFSSLLHKQAIAKHAVLEQENKYVEAVNEL 268


33B7485_10095B7485_10125Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_10095-225-3.264995DNA polymerase III subunit theta
B7485_10100-225-4.202213DNA polymerase III subunit epsilon
B7485_10105-126-3.800684oligopeptidase B
B7485_10110-128-4.690778hypothetical protein
B7485_10120-128-4.426508protein YebE
B7485_10125-121-3.755154hypothetical protein
34B7485_10215B7485_10380Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_10215025-3.822818Holliday junction branch migration protein RuvA
B7485_10220024-3.684929hypothetical protein
B7485_10225-121-2.766584hypothetical protein
B7485_10235125-1.713778crossover junction endodeoxyribonuclease RuvC
B7485_10245123-0.380313transcriptional regulator
B7485_10250124-0.997297NUDIX pyrophosphatase
B7485_10255024-3.548548aspartate--tRNA ligase
B7485_10260024-3.744179nicotinamidase
B7485_10265-121-4.073520IS110 family transposase
B7485_10270-119-2.663144preprotein translocase subunit SecY
B7485_10275017-1.978493DinI family protein
B7485_10280-118-0.581935E3 ubiquitin--protein ligase
B7485_102901190.594122hypothetical protein
B7485_10300015-1.496559Ail/Lom family protein
B7485_10305012-1.988373host specificity protein J
B7485_10310014-3.399325tail assembly protein
B7485_10315016-3.878289phage tail protein
B7485_10320-119-3.950150phage minor tail protein L
B7485_10325-118-3.315754phage tail protein
B7485_10330019-2.432725phage tail tape measure protein
B7485_10335224-1.762856phage tail assembly protein T
B7485_10340122-1.832217phage minor tail protein G
B7485_10345123-2.118800phage tail protein
B7485_10350223-3.346211phage tail protein
B7485_10355327-4.116750phage tail protein
B7485_10360335-5.591460phage tail protein
B7485_10365435-5.854103DNA-packaging protein FI
B7485_10370233-4.594129IS110 family transposase
B7485_10375132-3.834154hypothetical protein
B7485_10380029-4.473005****antiterminator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10520PilS_PF08805300.007 PilS N terminal
		>PilS_PF08805#PilS N terminal

Length = 185

Score = 29.5 bits (66), Expect = 0.007
Identities = 12/46 (26%), Positives = 18/46 (39%)

Query: 29 AASNCWSNHVGIIIGHNGEDFLVAESRVPLSTITTLSRFIKRSSNQ 74
+A N W V I + F V E+ VP + ++ SS
Sbjct: 110 SAKNPWGGSVTITTSSDKYSFNVVEANVPQKNCMAMVNALRSSSAI 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10595ENTEROVIROMP1342e-42 Enterobacterial virulence outer membrane protein si...
		>ENTEROVIROMP#Enterobacterial virulence outer membrane protein

signature.
Length = 171

Score = 134 bits (338), Expect = 2e-42
Identities = 63/200 (31%), Positives = 101/200 (50%), Gaps = 30/200 (15%)

Query: 1 MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPGSDDLNGINVKYRYEFT 60
M+K+ A + + A LA + + A+ ST++ GY + + + G N+KYRYE
Sbjct: 1 MKKI-ACLSALAAVLAFTAGTSVAA--TSTVTGGYAQSDAQGQMNK-MGGFNLKYRYEED 56

Query: 61 DT-LGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGV 119
++ LG++ SF+Y T S T D +N+++ + AG + R+N+W S Y + GV
Sbjct: 57 NSPLGVIGSFTY--------TEKSRTASSGDYNKNQYYGITAGPAYRINDWASIYGVVGV 108

Query: 120 AYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYE 179
Y + T T+ HD S+ ++GAG+QFNP E+VA+D +YE
Sbjct: 109 GYGKFQT--------TEYPTYKHD---------TSDYGFSYGAGLQFNPMENVALDFSYE 151

Query: 180 GSGSGDWRTDGFIVGVGYKF 199
S +I GVGY+F
Sbjct: 152 QSRIRSVDVGTWIAGVGYRF 171


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10625RTXTOXIND396e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 39.4 bits (92), Expect = 6e-05
Identities = 14/194 (7%), Positives = 43/194 (22%), Gaps = 12/194 (6%)

Query: 29 LNGAASDAERSSARMQRFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMARE 88
L ++A+ + Q + + Q S + + E
Sbjct: 127 LTALGAEADTLKTQSSL---LQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEE 183

Query: 89 VEQTRLRVDALNQKMREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQI 148
V + + + ++ Q + + +I+ + +
Sbjct: 184 VLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDD---F 240

Query: 149 RQARNSGGVGQQDYLALISEITAKTRALTQAE------EQATRQKAAFIRQLKEQATRQN 202
+ + + L ++ L + E + + + +
Sbjct: 241 SSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEI 300

Query: 203 LSSSELLRARAAQL 216
L L
Sbjct: 301 LDKLRQTTDNIGLL 314



Score = 38.7 bits (90), Expect = 1e-04
Identities = 25/224 (11%), Positives = 63/224 (28%), Gaps = 30/224 (13%)

Query: 545 NYQEQQKRRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAI 604
+ + + A L + + E+ ++ ++ D+ + E R I
Sbjct: 133 EADTLKTQSSLLQARLEQ-TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLI 191

Query: 605 KKNTRATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISD 664
K+ + Q+ Q E + R R+ + R+ D
Sbjct: 192 KEQ------------FSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDD 239

Query: 665 LDGKKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERA 724
L + I +L+ + + ++ L + + Q+ S++ +
Sbjct: 240 F--SSL---------LHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEE 288

Query: 725 LREQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSR 768
+ + + Q I +L + E +
Sbjct: 289 YQ-LVTQLFKN----EILDKLRQTTDNIGL-LTLELAKNEERQQ 326



Score = 32.5 bits (74), Expect = 0.010
Identities = 31/238 (13%), Positives = 69/238 (28%), Gaps = 42/238 (17%)

Query: 402 DPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETGKRTSDIDNNLN 461
+ A L +LEQ R RS + ++ L +E + + L
Sbjct: 128 TALGAEADTLKTQSSLLQARLEQTRYQILS-RSIELNKLPELKLPDEPYFQNVSEEEVLR 186

Query: 462 ALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYPWTNASTQVEYDQQ 521
+ S W Q + +N+ D A+ + +I R + ++
Sbjct: 187 LTSLIKEQFSTWQNQKYQKELNL---DKKRAERLTVLARINRYEN--------LSRVEKS 235

Query: 522 RLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNETEAARHQREIARINAM 581
RL+D L +A A+ EQ+ + L +++ +
Sbjct: 236 RLDDFSS------LLHKQAIAKHAVLEQENKYVEAVNELRVY-----------KSQLEQI 278

Query: 582 QYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYSQQQAQVEGQIAAARQ 639
+ + ++ + + K + T + ++A +
Sbjct: 279 ESEILSAKEEYQLVTQLFKNEILDKLRQTT-------------DNIGLLTLELAKNEE 323



Score = 31.0 bits (70), Expect = 0.030
Identities = 26/185 (14%), Positives = 57/185 (30%), Gaps = 13/185 (7%)

Query: 13 IDAAEFKNEIPRIKNLLNGAASDAERSSARMQRFMERQTQAARQTMQAASSAATAASAHA 72
+ E + +E R+ ++ Q + A
Sbjct: 157 SRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAER 216

Query: 73 QTVEKNARAHERMAREVEQTRLRVDALNQKMREEQAQARALAEAQDKAAAAFYRQIDSVK 132
TV +E ++R VE++RL + +QA A+ Q+ ++
Sbjct: 217 LTVLARINRYENLSR-VEKSRL---DDFSSLLHKQAIAKHAVLEQENKYVEAVNEL---- 268

Query: 133 QAGAGLQELQRIQQQIRQARNSGGVGQQDYLALISEITAKTRA-LTQAEEQATRQKAAFI 191
+L++I+ +I A+ + Q + I + +T + + K
Sbjct: 269 --RVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLE--LAKNEER 324

Query: 192 RQLKE 196
+Q
Sbjct: 325 QQASV 329


35B7485_10530B7485_10735Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_105302222.405951chemotaxis response regulator protein-glutamate
B7485_105351202.170504chemotaxis protein-glutamate
B7485_105401221.792233methyl-accepting chemotaxis protein
B7485_105450301.547231methyl-accepting chemotaxis protein II
B7485_10555531-3.663511chemotaxis protein CheW
B7485_10560528-3.086989flagellar motor protein MotB
B7485_10565429-0.877041flagellar motor stator protein MotA
B7485_105704260.129849flagellar transcriptional regulator FlhC
B7485_105753191.883638flagellar transcriptional regulator FlhD
B7485_105803233.899620universal stress protein UspC
B7485_105904234.449126trehalose-phosphatase
B7485_106002264.370926arabinose ABC transporter permease
B7485_106053274.481271arabinose import ATP-binding protein AraG
B7485_106103284.092937non-heme ferritin
B7485_106152253.580671hypothetical protein
B7485_106203253.264936hypothetical protein
B7485_106253263.433885hypothetical protein
B7485_106357291.913250non-heme ferritin
B7485_106404213.422623hypothetical protein
B7485_106452252.416449tyrosine-specific transporter
B7485_106501251.003558YecA family protein
B7485_106551250.400072***CDP-diacylglycerol--glycerol-3-phosphate
B7485_106601250.088813excinuclease ABC subunit C
B7485_10665128-0.534388DNA-binding response regulator
B7485_10670129-4.021888hypothetical protein
B7485_10700024-1.656947transcriptional regulator SdiA
B7485_10705122-1.746053L-cystine ABC transporter ATP-binding protein
B7485_10710124-1.835891amino acid ABC transporter permease
B7485_10715123-1.525021D-cysteine desulfhydrase
B7485_10720223-1.032505cystine ABC transporter substrate-binding
B7485_10725323-0.906321flagellar regulatory protein FliZ
B7485_10730426-1.593948RNA polymerase sigma factor FliA
B7485_10735427-2.049256flagellin FliC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10875HTHFIS659e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 65.2 bits (159), Expect = 9e-14
Identities = 35/188 (18%), Positives = 72/188 (38%), Gaps = 23/188 (12%)

Query: 1 MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMP 60
M+ +L DD A +R ++ + ++ V + I + D++ DV MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 RMDGLDFLEKLMRLRPMPVVMVSSLTGKGS-EVTLRALELGAIDFVTKPQLGIREGMLAY 119
+ D L ++ + RP V+V ++ + + ++A E GA D++ KP + E +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLV--MSAQNTFMTAIKASEKGAYDYLPKP-FDLTELIGII 115

Query: 120 SEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQPLP 179
+AE R +K + + +G S E R + + +
Sbjct: 116 GRALAEPKRRPSKLEDDSQDGMP-----------------LVGRSAAMQEIYRVLARLMQ 158

Query: 180 LSSPALLI 187
++
Sbjct: 159 TDLTLMIT 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10905PF05272310.009 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.009
Identities = 22/93 (23%), Positives = 35/93 (37%), Gaps = 11/93 (11%)

Query: 46 LISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPNIEE 105
L +SSP A P + G + ++ PGGGDD GE +++
Sbjct: 384 LADVSSPTAAAGGAGGGEPPKKRDPSAG---AGTDPGGPGGGDD-----GEDPFGEWLDD 435

Query: 106 LKKRM---EQSRLRKLRGDLDQLIESDPKLRAL 135
R+ + L+ R L + + S P L
Sbjct: 436 EVARLRLRGRWLLKPRRAALIEALRSAPALAGC 468


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10910PF05844330.001 YopD protein
		>PF05844#YopD protein

Length = 295

Score = 33.1 bits (75), Expect = 0.001
Identities = 12/28 (42%), Positives = 22/28 (78%), Gaps = 2/28 (7%)

Query: 76 MDLLALLYRLMAKSRQMGMFSLERDIEN 103
++LL +L+R+ K+R++G+ L+RD EN
Sbjct: 74 VELLLILFRIAQKARELGV--LQRDNEN 99


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10950PF05272300.029 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.029
Identities = 15/40 (37%), Positives = 16/40 (40%), Gaps = 10/40 (25%)

Query: 18 PGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNY 57
PG K FD L G G GKSTL+ L G
Sbjct: 591 PGCK------FDY----SVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10995SECA609e-13 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 60.3 bits (146), Expect = 9e-13
Identities = 27/70 (38%), Positives = 31/70 (44%), Gaps = 5/70 (7%)

Query: 155 RVEKMSPEAFEESVDAIRLAALDLH---AYWMAHPQEKAVQQPI--KAEEKPGRNDPCPC 209
+V+ PE EE R+ A L A E K GRNDPCPC
Sbjct: 828 KVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPC 887

Query: 210 GSGKKFKQCC 219
GSGKK+KQC
Sbjct: 888 GSGKKYKQCH 897


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11025HTHFIS755e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.3 bits (185), Expect = 5e-18
Identities = 23/113 (20%), Positives = 47/113 (41%), Gaps = 2/113 (1%)

Query: 4 VLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCRTNAVDVVLMDMSMPGIGGL 63
+L+ DD +R + + L G V ++ +W D+V+ D+ MP
Sbjct: 6 ILVADDDAAIRTVLNQALSR-AGYDVRITSN-AATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 64 EATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYLSKGAAPQEVVSAIR 116
+ +I ++ D+ +++++ K + GA YL K E++ I
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11070FLAGELLIN2349e-73 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 234 bits (599), Expect = 9e-73
Identities = 260/551 (47%), Positives = 311/551 (56%), Gaps = 47/551 (8%)

Query: 2 AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 61
AQVINTNSLSL+TQNN+NK+QS+LSS+IERLSSGLRINSAKDDAAGQAIANRFTSNIKGL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 TQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTGTNSDSDLDSIQDEIKSRLD 121
TQA+RNANDGIS+AQTTEGAL+EINNNLQR+REL+VQA+ GTNSDSDL SIQDEI+ RL+
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDLKKIDSDTLGLNGFNVNGGGAV 181
EIDRVS QTQFNGV VL++D MKIQVGANDG+TITIDL+KID +LGL+GFNVNG
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEA 180

Query: 182 A---NTAASKADLVAANATVVGNKYTVSAGYDAAKASDLLAGVSDGDTVQATINNGFGTA 238
++ K V NKY V A V D V A N T
Sbjct: 181 TVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAA-NGQLTTD 239

Query: 239 ASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDTAKGTITIDGSAQDVQISSDGKI 298
+ N D + S T + A GDT +GK+
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKV 299

Query: 299 TASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAANNTKATTIDIGGTSISFTGNSTTPD 358
+ T NG +LT A ++ AAN AT S T D
Sbjct: 300 ST--------------TINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFD 345

Query: 359 TITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGYSVNGTTGAVTKGVDSVYVDNNEALTT 418
T + + D A + S V+ + G +
Sbjct: 346 DKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLA---------------- 389

Query: 419 SDTVDFYLQDDGSVTNGSGKAVYKDADGKLTTDAETKAATTADPLKALDEAISSIDKFRS 478
+ DA +TA+PL ++D A+S +D RS
Sbjct: 390 -------------GKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDSALSKVDAVRS 436

Query: 479 SLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKAN 538
SLGA+QNR DSA+TNL NT TNL+ A+SRI+DADYATEVSNMSKAQI+QQAG SVLA+AN
Sbjct: 437 SLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQAN 496

Query: 539 QVPQQVLSLLQ 549
QVPQ VLSLL+
Sbjct: 497 QVPQNVLSLLR 507


36B7485_11115B7485_11810Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_11115122-4.139737*protein MtfA
B7485_11120123-6.013776*serine transporter
B7485_11125329-7.363037IS3 family transposase
B7485_11135640-10.199040hypothetical protein
B7485_11145641-8.906967hypothetical protein
B7485_11155-218-0.014335hypothetical protein
B7485_111650152.557070hypothetical protein
B7485_111701153.623006invasion protein
B7485_111750164.454044hypothetical protein
B7485_111851184.073178helix-turn-helix domain-containing protein
B7485_11195-1173.255906holin
B7485_11205-2161.920069****hypothetical protein
B7485_11215-2211.883192hypothetical protein
B7485_112200180.560946hypothetical protein
B7485_11225117-2.425440Hok/Gef family protein
B7485_11230118-3.270151hypothetical protein
B7485_11235018-4.115838hypothetical protein
B7485_11240022-5.902613DUF4224 domain-containing protein
B7485_11245-124-6.400533integrase
B7485_11250-223-4.951911*protein MtfA
B7485_11255-117-0.362450*serine transporter
B7485_11260-217-0.433929IS3 family transposase
B7485_11265-218-0.302464hypothetical protein
B7485_11270-2160.163060hypothetical protein
B7485_11275-1161.055104hypothetical protein
B7485_112800181.090058IS66 family transposase
B7485_112902171.400041transposase
B7485_112952191.534776general secretion pathway protein GspL
B7485_113000160.967086integrase
B7485_11305015-0.566694exodeoxyribonuclease I
B7485_11315226-1.233101hypothetical protein
B7485_11325327-3.007418YeeE/YedE family protein
B7485_11330329-2.767248APC family permease
B7485_11340337-7.219798LysR family transcriptional regulator
B7485_11345239-7.259217NAD(P)-dependent oxidoreductase
B7485_11350-130-5.141726Txe/YoeB family addiction module toxin
B7485_11355028-4.967211antitoxin YefM
B7485_11360031-7.139855ATP phosphoribosyltransferase
B7485_11365229-6.654418histidinol dehydrogenase
B7485_11370126-6.588606histidinol-phosphate transaminase
B7485_11375023-5.771403bifunctional imidazole glycerol-phosphate
B7485_11385734-7.715770imidazole glycerol phosphate synthase subunit
B7485_11390734-5.8080961-(5-phosphoribosyl)-5-[(5-
B7485_11395728-3.651901imidazole glycerol phosphate synthase cyclase
B7485_11400729-2.956611bifunctional phosphoribosyl-AMP
B7485_11410626-1.290093chain length determinant protein
B7485_114154230.207386phosphogluconate dehydrogenase
B7485_11420423-0.546250hypothetical protein
B7485_11425326-2.355008hypothetical protein
B7485_11430026-2.386750hypothetical protein
B7485_11435123-1.687352hypothetical protein
B7485_11440123-0.976175protein RfbJ
B7485_11465123-0.463344O-antigen polymerase
B7485_11470-125-0.278538dTDP-rhamnosyl transferase RfbG
B7485_11475-1240.327611rhamnosyltransferase
B7485_11485128-3.131107flippase
B7485_11490329-6.546884dTDP-4-dehydrorhamnose 3,5-epimerase
B7485_11500123-5.096608glucose-1-phosphate thymidylyltransferase
B7485_11505122-3.509807NAD(P)-dependent oxidoreductase
B7485_11510022-3.310870dTDP-glucose 4,6-dehydratase
B7485_11525120-1.733370UTP--glucose-1-phosphate uridylyltransferase
B7485_11540226-2.004020colanic acid biosynthesis protein WcaM
B7485_11545227-2.580486colanic acid biosynthesis glycosyltransferase
B7485_11550426-1.068465colanic acid biosynthesis pyruvyl transferase
B7485_115604240.690173lipopolysaccharide biosynthesis protein
B7485_115653240.773480undecaprenyl-phosphate glucose
B7485_115753230.989885phosphomannomutase CpsG
B7485_115803330.309218mannose-1-phosphate
B7485_11590-137-1.706707colanic acid biosynthesis glycosyltransferase
B7485_11600038-2.805001GDP-mannose mannosyl hydrolase
B7485_11605-137-3.303699GDP-mannose 4,6-dehydratase
B7485_11610035-4.042937colanic acid biosynthesis acetyltransferase
B7485_11615833-4.197441transposase
B7485_11620833-3.944608colanic acid biosynthesis glycosyltransferase
B7485_11625829-2.881031serine acetyltransferase
B7485_11630728-1.661970colanic acid biosynthesis glycosyltransferase
B7485_116406251.002522tyrosine-protein kinase
B7485_116455230.395258protein tyrosine phosphatase
B7485_11650423-0.368160polysaccharide export protein Wza
B7485_11660026-2.386750transporter
B7485_11665123-1.687352outer membrane assembly protein AsmA
B7485_11670123-0.976175dCTP deaminase
B7485_11695123-0.463344uridine kinase
B7485_11700-125-0.278538hypothetical protein
B7485_11705-1240.327611IS110 family transposase
B7485_11710023-0.946238hypothetical protein
B7485_11720329-6.546884protein phosphatase 2C domain-containing
B7485_11725328-7.476509helix-hairpin-helix domain-containing protein
B7485_11735122-3.509807molecular chaperone
B7485_11740022-3.310870molecular chaperone
B7485_11745122-3.064378molecular chaperone
B7485_11765119-0.8533683-methyladenine DNA glycosylase 2
B7485_11775227-2.580486IS110 family transposase
B7485_11785529-0.184975multidrug transporter subunit MdtA
B7485_117904220.687578multidrug transporter subunit MdtC
B7485_117953220.734871multidrug transporter subunit MdtD
B7485_118053230.976314two-component sensor histidine kinase
B7485_118103270.349129DNA-binding response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11545TCRTETA280.024 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 27.9 bits (62), Expect = 0.024
Identities = 13/52 (25%), Positives = 21/52 (40%), Gaps = 4/52 (7%)

Query: 105 PCFAWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANI 156
P L+DRFGRR V + + A W+++ ++A I
Sbjct: 61 PVLGALSDRFGRRPVLLVSLAGAAVDYAIMATA----PFLWVLYIGRIVAGI 108


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11665HTHTETR280.002 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 28.1 bits (62), Expect = 0.002
Identities = 9/34 (26%), Positives = 17/34 (50%), Gaps = 3/34 (8%)

Query: 19 ALRLRFE---DKLTIRAIAQRLGLSHSTIHTLFQ 49
ALRL + ++ IA+ G++ I+ F+
Sbjct: 20 ALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFK 53


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11670FLAGELLIN250.029 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 25.0 bits (54), Expect = 0.029
Identities = 14/77 (18%), Positives = 31/77 (40%), Gaps = 9/77 (11%)

Query: 2 KSMDKISTGIAYGTSAGSAGYWFL--------QWLDQVSPSQWAAIGVLGSLVLGFLTYL 53
+++++S+G+ ++ A + + L Q S + I + G L +
Sbjct: 26 SAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNANDGISIA-QTTEGALNEI 84

Query: 54 TNLYFKIREDKRKAARG 70
N ++RE +A G
Sbjct: 85 NNNLQRVRELSVQATNG 101


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11725HOKGEFTOXIC645e-18 Hok/Gef cell toxic protein family signature.
		>HOKGEFTOXIC#Hok/Gef cell toxic protein family signature.

Length = 52

Score = 64.1 bits (156), Expect = 5e-18
Identities = 19/46 (41%), Positives = 32/46 (69%)

Query: 23 QKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEP 68
+ +++ ++++CLT+++ +TRK LCE+R R G EVA F AYE
Sbjct: 5 RSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYES 50


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11775TCRTETA280.024 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 27.9 bits (62), Expect = 0.024
Identities = 13/52 (25%), Positives = 21/52 (40%), Gaps = 4/52 (7%)

Query: 105 PCFAWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANI 156
P L+DRFGRR V + + A W+++ ++A I
Sbjct: 61 PVLGALSDRFGRRPVLLVSLAGAAVDYAIMATA----PFLWVLYIGRIVAGI 108


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11805RTXTOXIND417e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.4 bits (97), Expect = 7e-06
Identities = 9/74 (12%), Positives = 33/74 (44%), Gaps = 1/74 (1%)

Query: 16 LRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLFGQSSEKKRHKLENQIRQAEKRLS 75
+ +Q+++ + ++ Y+ ++E++++++ + + K L+ ++RQ +
Sbjct: 254 VLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILD-KLRQTTDNIG 312

Query: 76 ELENRLNTARNLLE 89
L L +
Sbjct: 313 LLTLELAKNEERQQ 326


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11845PF01206574e-15 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 57.5 bits (139), Expect = 4e-15
Identities = 15/72 (20%), Positives = 35/72 (48%), Gaps = 1/72 (1%)

Query: 4 KKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATEAIPQWAAEEGHAITDYQQI 63
+ LD CP P+++AK LA M +G+ L + + + ++ + GH + + ++
Sbjct: 6 QSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKEE 65

Query: 64 GDAAWSITVQKA 75
+ +++A
Sbjct: 66 DG-TYHFRLKRA 76


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11865NUCEPIMERASE290.025 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 28.6 bits (64), Expect = 0.025
Identities = 25/126 (19%), Positives = 51/126 (40%), Gaps = 16/126 (12%)

Query: 95 LVDSALAHRIPRIIFTSSTSVYGDAQG---TVKETT--PRNPVTNSGRVLEELEDWLHNL 149
+++ ++I +++ SS+SVYG + + ++ P + + + E + +L
Sbjct: 109 ILEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHL 168

Query: 150 PGTSVDILRLAGLVGP-GRHPGRFF-------AGKTAP---DGEHGVNLVHLEDVIGAIT 198
G LR + GP GR F GK+ G+ + +++D+ AI
Sbjct: 169 YGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAII 228

Query: 199 LLLQAP 204
L
Sbjct: 229 RLQDVI 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12005NUCEPIMERASE475e-08 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 46.7 bits (111), Expect = 5e-08
Identities = 33/175 (18%), Positives = 64/175 (36%), Gaps = 35/175 (20%)

Query: 1 MNILLFGKTGQVGWELQRALAPLGN-LIALDVHSTDY--------------------CGD 39
M L+ G G +G+ + + L G+ ++ +D + Y D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 40 FSNPEGVAETVKKIRPDVIVNAAAHTAVDKAESEP------NFAQLLNATCVEAIAKAAN 93
++ EG+ + + + + AV + P N LN + +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLN---ILEGCRHNK 117

Query: 94 EVGAWVIHYSTDYVFPGNGDTPWLETDATA-PLNVYGETKLAGEKALQEHCAKHL 147
+ +++ S+ V+ N P+ D+ P+++Y TK A E L H HL
Sbjct: 118 -IQH-LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANE--LMAHTYSHL 168


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12010NUCEPIMERASE1847e-58 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 184 bits (470), Expect = 7e-58
Identities = 89/360 (24%), Positives = 149/360 (41%), Gaps = 48/360 (13%)

Query: 1 MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLT--YAGNL-ESLADVSDSERYAFEHA 57
MK LVTG AGFIG V + ++ VV +D L Y +L ++ ++ + F
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAG-HQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59

Query: 58 DICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSA 117
D+ D M+ +FA + V V S+ P A+ ++N+ G +LE R+
Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN--- 116

Query: 118 LNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTAYAPSSPYSASKASSDHL 177
K + S+ VYG N +P T+ + P S Y+A+K +++ +
Sbjct: 117 ------KIQHLLYASSSSVYGL---------NRKMPFSTDDSVDHPVSLYAATKKANELM 161

Query: 178 VRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGNQIRDWLYVE 237
+ YGLP YGP+ P+ + LEGK++ +Y G RD+ Y++
Sbjct: 162 AHTYSHLYGLPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYID 221

Query: 238 D-------------HARALYTVVTEGKA-----GETYNIGGHNEKKNIDVVLTICDLLDE 279
D HA +TV T A YNIG + + +D + + D L
Sbjct: 222 DIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALG- 280

Query: 280 IVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWYLANTN 339
+ +K+ +PG + D + +G+ P+ T + G++ V WY
Sbjct: 281 -IEAKKNMLPL------QPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDFYK 333


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12070NUCEPIMERASE1041e-27 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 104 bits (262), Expect = 1e-27
Identities = 76/353 (21%), Positives = 122/353 (34%), Gaps = 42/353 (11%)

Query: 6 LITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPH--------TCNPK 57
L+TG G G ++++ LLE G++V GI + N Y D P
Sbjct: 4 LVTGAAGFIGFHVSKRLLEAGHQVVGI----DNLND------YYDVSLKQARLELLAQPG 53

Query: 58 FHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAI 117
F H DL+D +T + + V+ V S E+P AD + G L +LE
Sbjct: 54 FQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGC 113

Query: 118 RFLGLEKKTRFYQASTSELYGLVQEIPQKETTPF-YPRSPYAVAKLYAYWITVNYRESYG 176
R ++ AS+S +YGL +++P +P S YA K + Y YG
Sbjct: 114 RHNKIQ---HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170

Query: 177 MYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKM 236
+ A F P K T+A+ G +Y RD+ + D +
Sbjct: 171 LPATGLRFFTVYGPWGRPDMALFKFTKAMLE---GKSIDVY-NYGKMKRDFTYIDDIAEA 226

Query: 237 QWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAP 296
+ D +G + E + DA
Sbjct: 227 IIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIG------NSSPVELMDYIQAL-EDAL 279

Query: 297 GVKPGDVIIAVDPRY--FRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMV 347
G++ +P +V D +E +G+ PE T+++ V V
Sbjct: 280 GIE-------AKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFV 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12175SHAPEPROTEIN362e-05 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 35.5 bits (82), Expect = 2e-05
Identities = 30/127 (23%), Positives = 52/127 (40%), Gaps = 20/127 (15%)

Query: 18 RSAEECKIALSSV--AETRASLPFISNELAT------LISQRGLESALSQPLARILEQVQ 69
+AE K + S + + LA ++ + AL +PL I+ V
Sbjct: 213 ATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVM 272

Query: 70 LALDNAQEKPDV--------IYLTGGSARSPLIKKALAEQLPGIPIAGGDD-FGSVTAGL 120
+AL+ Q P++ + LTGG A + + L E+ GIP+ +D V G
Sbjct: 273 VALE--QCPPELASDISERGMVLTGGGALLRNLDRLLMEET-GIPVVVAEDPLTCVARGG 329

Query: 121 ARWAEVV 127
+ E++
Sbjct: 330 GKALEMI 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12180SHAPEPROTEIN503e-09 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 50.1 bits (120), Expect = 3e-09
Identities = 32/129 (24%), Positives = 57/129 (44%), Gaps = 20/129 (15%)

Query: 132 AMMLH-IRQQAQAQLPEAITQAVIGRPINFQGLGGDEANAQAQGILERAAKRAGFKDVVF 190
M+ H I+Q + ++ P+ + E A + +A+ AG ++V
Sbjct: 89 KMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQV---ERRA-----IRESAQGAGAREVFL 140

Query: 191 QYEPVAAGLDYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLLGHSGYRI 250
EP+AA + + E +VVDIGGGTT+ +++ + ++ S RI
Sbjct: 141 IEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN-----------GVVYSSSVRI 189

Query: 251 GGNDLDIAL 259
GG+ D A+
Sbjct: 190 GGDRFDEAI 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12200RTXTOXIND445e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.0 bits (104), Expect = 5e-07
Identities = 33/167 (19%), Positives = 64/167 (38%), Gaps = 11/167 (6%)

Query: 61 ALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIKADEAS 120
+ +L K+ L ++ AK +L + L + T +
Sbjct: 260 KYVEAVNELRVYKSQLEQIESEILS----AKEEYQLVTQLFKNEILDKLRQTTDNIGLLT 315

Query: 121 --VASAQLQLDWSRITAPVDGRV-GLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTLPE 177
+A + + S I APV +V LK G +++ +T +V++ + +++ +
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETL-MVIVPEDDTLEVTALVQN 374

Query: 178 SDIATVVQAQKAGKPLMVEAWDRTNSKKL-SEGTLLSLDNQIDATTG 223
DI + Q A + VEA+ T L + ++LD D G
Sbjct: 375 KDIGFINVGQNA--IIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLG 419



Score = 43.7 bits (103), Expect = 8e-07
Identities = 21/122 (17%), Positives = 47/122 (38%), Gaps = 13/122 (10%)

Query: 15 GTITAA-NTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDK 73
G +T + + ++ + + + +EG+ V+ GD+L ++ + K +
Sbjct: 88 GKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTAL-------GAEADTLKTQ 140

Query: 74 ATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRI 133
++L AR + RYQ L+++ EL+ L E + L +
Sbjct: 141 SSLLQARLEQTRYQILSRS-----IELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQF 195

Query: 134 TA 135
+
Sbjct: 196 ST 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12210ACRIFLAVINRP9070.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 907 bits (2345), Expect = 0.0
Identities = 287/1035 (27%), Positives = 502/1035 (48%), Gaps = 40/1035 (3%)

Query: 6 LFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPVIMVSASLPGASPETMASSVAT 65
FI RP+ +L++ + + G L LPVA P + P + VSA+ PGA +T+ +V
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 66 PLERSLGRIAGVSEMTSSS-SLGSTRIILQFDFDRDINGAARDVQAAINAAQSLLPSGMP 124
+E+++ I + M+S+S S GS I L F D + A VQ + A LLP +
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 125 SRPTYRKANPSDAPIMILTLTSDT--YSQGELYDFASTQLAPTISQIDGVGDVDVGGSSL 182
+ S + +M+ SD +Q ++ D+ ++ + T+S+++GVGDV + G+
Sbjct: 124 -QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY 182

Query: 183 PAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG------ALEDGTHRWQIQTNDELK 236
A+R+ L L ++ DV + N + G AL I K
Sbjct: 183 -AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 237 TAAEYQPLIIHYN-NGGAVRLGDVATVTDSVQDVRNAGMTNAKPAILLMIRKLPEANIIQ 295
E+ + + N +G VRL DVA V ++ N KPA L I+ AN +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 296 TVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEVEQTLIISVALVILVVFLFLRS 355
T +I+AKL ELQ P + + D +P ++ S+ EV +TL ++ LV LV++LFL++
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 356 GRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMALTIATGFVVDDAIVVLENIARHL- 414
RAT+IP +AVPV L+GTFA + G+S+N L++ + +A G +VDDAIVV+EN+ R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 EAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLLLMGGLPGRLLREFAVTLSVAIGIS 474
E + P +A + ++ ++ +++ L AVF+P+ GG G + R+F++T+ A+ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 475 LLVSLTLTPMMCGWMLKASKPREQKRLRGFG----RMLVALQQGYGKSLKWVLNHTRLVG 530
+LV+L LTP +C +LK + GF Y S+ +L T
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 531 VVLLGTIALNI----SIPKTFFPEQDTGVLMGGIQADQSISFQ----AMRGKLQDFMKII 582
++ +A + +P +F PE+D GV + IQ + + + ++K
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 583 RD-DPAVDNVTGFT-GGSRVNSGMMFITLKPRDERS---ETAQQIIDRLRVKLAKEPGAN 637
+ +V V GF+ G N+GM F++LKP +ER+ +A+ +I R +++L K
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 638 LFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLATL-----PELADVNSD 692
+ + I G + ++ L D + + R +L + L V +
Sbjct: 662 VIPFNMPAIVELGTATGFDFE---LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 693 QQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRY 752
++ A+ L D++ LG+ + N ++ A G ++ K+ ++ D ++
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 753 TQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAASTISFNLPTGKSLSD 812
++K++V + G+ +P S F + + I G S D
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 813 ASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAIATVYIVLGILYESYVH 872
A A ++ ++L P+ + + G + + + N L+ + V++ L LYES+
Sbjct: 839 AMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSI 896

Query: 873 PLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRHGN 932
P++++ +P VG LLA LFN + ++G++ IG+ KNAI++V+FA +
Sbjct: 897 PVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 933 LTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLEITIVGGLVMSQLL 992
EA A +R RPI+MT+LA + G LPL +S G GS + + I ++GG+V + LL
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 993 TLYTTPVVYLFFDRL 1007
++ PV ++ R
Sbjct: 1017 AIFFVPVFFVVIRRC 1031



Score = 78.3 bits (193), Expect = 1e-16
Identities = 77/446 (17%), Positives = 162/446 (36%), Gaps = 26/446 (5%)

Query: 588 VDNVTGFTGGS-RVNSGMMFITLKPRDERSETAQQIIDRLRVKLAKEPGANLFLMAVQDI 646
+DN+ + S S + +T + + Q+ ++L++ P + Q I
Sbjct: 72 IDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE----VQQQGI 127

Query: 647 RVGGRQSNASYQYTLLSDDLAALREW-----EPKIRKKLATLPELADVNSDQQDNGAE-- 699
V S+ +SD+ ++ ++ L+ L + DV GA+
Sbjct: 128 SVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL----FGAQYA 183

Query: 700 MNLVYDRDTMARLGID----VQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYTQD 755
M + D D + + + + + + T P Q + R+
Sbjct: 184 MRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNP 243

Query: 756 ISALEKMFVINNEGKAIPLSYFAK--WQPANAPLSVNHQGLSAASTISFNLPTGKSLSDA 813
+ +N++G + L A+ N + G AA +L D
Sbjct: 244 EEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL-DT 302

Query: 814 SAAIDRAMTQL--GVPSTVRGSFA-GTAQVFQETMNSQVILIIAAIATVYIVLGILYESY 870
+ AI + +L P ++ + T Q +++ V + AI V++V+ + ++
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 871 VHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRH 930
L +P +G L F + + + G++L IG++ +AI++V+
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 931 GNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLEITIVGGLVMSQ 990
L P+EA ++ ++ + +P+ GG + + ITIV + +S
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 991 LLTLYTTPVVYLFFDRLRLRFSRKPK 1016
L+ L TP + + + K
Sbjct: 483 LVALILTPALCATLLKPVSAEHHENK 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12215TCRTETB1251e-33 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 125 bits (315), Expect = 1e-33
Identities = 97/429 (22%), Positives = 187/429 (43%), Gaps = 23/429 (5%)

Query: 20 FMQSLDTTIVNTALPSMAQSLGESPLHMLMVIVSYVLTVAVMLPASGWLADKVGVRNIFF 79
F L+ ++N +LP +A + P V +++LT ++ G L+D++G++ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 80 TAIVLFTLGSLFCALSGTLNELL-LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTF 138
I++ GS+ + + LL +AR +QG G A + + V + +P+E A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 139 VTLPGQVGPLLGPALGGLLVEYASWHWIFLINIPVGIIGAIATLM-LMPNYTMQTRRFDL 197
+ +G +GPA+GG++ Y HW +L+ IP+ I + LM L+ FD+
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 198 SGFLLLAVGMAVLTLALDGSKGTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKL 257
G +L++VG+ L + + V V++ ++++ H R L
Sbjct: 202 KGIILMSVGIVFFMLF---------TTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGL 252

Query: 258 FRTRTFSLGLAGSFAGRIGSGMLPFMTPVFLQIGLGFSPFHAG-LMMIPMVLGSMGMKRI 316
+ F +G+ M P ++ S G +++ P + + I
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312

Query: 317 VVQVVNRFGYRRVLVATTLGLSLVTLLFMTTALL----GWYYVLPFVLFLQGMVNSTRFS 372
+V+R G VL +G++ +++ F+T + L W+ + V L G+ S +
Sbjct: 313 GGILVDRRGPLYVL---NIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL--SFTKT 367

Query: 373 SMNTLTLKDLPDNLASSGNSLLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVF 432
++T+ L A +G SLL+ LS G+ I G LL + + Q+ +
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTY 427

Query: 433 MYTWLSMAF 441
+Y+ L + F
Sbjct: 428 LYSNLLLLF 436


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12220BCTERIALGSPF310.009 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 31.3 bits (71), Expect = 0.009
Identities = 27/93 (29%), Positives = 34/93 (36%), Gaps = 27/93 (29%)

Query: 173 LATLLAALATFLLA-------------RGLLAPVKRLVDGTHKLAAGDFTTRVTPTSEDE 219
LATL+AA A L+A V+ V H LA + P S +
Sbjct: 77 LATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLAD---AMKCFPGSFER 133

Query: 220 L-----------GKLAQDFNQLASTLEKNQQMR 241
L G L N+LA E+ QQMR
Sbjct: 134 LYCAMVAAGETSGHLDAVLNRLADYTEQRQQMR 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12225HTHFIS766e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.6 bits (186), Expect = 6e-18
Identities = 28/136 (20%), Positives = 65/136 (47%), Gaps = 1/136 (0%)

Query: 11 PRILIVEDEPKLGQLLIDYLRAASYAPTLISHGDQVLAYVRQTPPDLILLDLMLPGTDGL 70
IL+ +D+ + +L L A Y + S+ + ++ DL++ D+++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 71 TLCREIR-RFSDVPIVMVTAKIEEIDRLLGLEIGADDYICKPYSPREVVARVKTILRRCK 129
L I+ D+P+++++A+ + + E GA DY+ KP+ E++ + L K
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 130 PQRELQQQDAESPLII 145
+ + D++ + +
Sbjct: 124 RRPSKLEDDSQDGMPL 139


37B7485_11885B7485_12045Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_11885-1243.347155fructose-bisphosphate aldolase
B7485_118950233.281032MFS transporter
B7485_11905-1200.320018ADP-ribosylglycohydrolase
B7485_11915-115-2.288244ADP-ribosylglycohydrolase family protein
B7485_11920014-4.378546kinase
B7485_11925119-6.631063transcriptional regulator
B7485_11930228-8.834724bifunctional hydroxymethylpyrimidine
B7485_11935332-10.295195hydroxyethylthiazole kinase
B7485_11940436-11.442248transcriptional repressor RcnR to maintain
B7485_11950660-20.003591sodium:proton antiporter
B7485_11955658-20.548436sodium:proton antiporter
B7485_11965759-20.224304heavy metal resistance protein
B7485_11970758-19.973825fimbrial biogenesis outer membrane usher
B7485_11975555-18.001766fimbrial assembly protein
B7485_11985243-11.594976hypothetical protein
B7485_11990132-8.560207hypothetical protein
B7485_11995-119-5.431628hypothetical protein
B7485_12000-113-3.172598protein mrp
B7485_12005-212-1.482799methionine--tRNA ligase
B7485_12010-313-0.617341MolR family transcriptional regulator
B7485_12015-2180.867922transposase
B7485_12020-1211.368706MolR family transcriptional regulator
B7485_12025-1232.635435hypothetical protein
B7485_120300232.749847MoxR family ATPase
B7485_12035-1222.870950hypothetical protein
B7485_12040-1233.173773hypothetical protein
B7485_12045-1233.280975hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12295TCRTETA355e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 35.2 bits (81), Expect = 5e-04
Identities = 53/268 (19%), Positives = 89/268 (33%), Gaps = 17/268 (6%)

Query: 29 LSKSGFSAGEIGWSYACTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQ 88
L S G A A+ ++G+++DRF ++ + ++ AGA + Y
Sbjct: 35 LVHSNDVTAHYGILLALYALMQFACAPVLGALSDRF--GRRPVLLVSLAGAAVDYAI--- 89

Query: 89 TTFAGFFPLLLAYSLTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIG-WIASGLACGF 147
A F +L + T A T ++A A + D+ R R G + G+ G
Sbjct: 90 MATAPFLWVLYIGRIVAGITGA-TGAVAGAYIADITDGDERARHFGFMSACFGFGMVAG- 147

Query: 148 LPQMLGY-ADISPTNIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILL 206
P + G SP + P A + L + FL K + + L A
Sbjct: 148 -PVLGGLMGGFSP-HAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRW 205

Query: 207 RDKN------FLVFFFCSFLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFML 260
VFF + +P A + IF G + +
Sbjct: 206 ARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAM 265

Query: 261 ALPFFTKRFGIKKVLLLGLVTAAICYGF 288
R G ++ L+LG++ Y
Sbjct: 266 ITGPVAARLGERRALMLGMIADGTGYIL 293



Score = 33.6 bits (77), Expect = 0.001
Identities = 32/153 (20%), Positives = 53/153 (34%), Gaps = 20/153 (13%)

Query: 253 FSEIFFMLALPFFTKRFGIKKVLLLGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGV 312
+ L + RFG + VLL+ L AA+ Y +L++G ++ G+
Sbjct: 54 LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAP-----FLWVLYIGRIVAGI 108

Query: 313 SYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVN 372
+ V D R G ++ C GFG + G LGG+M F+ P
Sbjct: 109 TGATGAVAGAYIAD-ITDGDERARHFGFMS-ACFGFGMVAGPVLGGLMGG--FSPHAP-- 162

Query: 373 GLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDN 405
+ A + + + ES
Sbjct: 163 ---------FFAAAALNGLNFLTGCFLLPESHK 186



Score = 28.6 bits (64), Expect = 0.048
Identities = 22/114 (19%), Positives = 43/114 (37%), Gaps = 4/114 (3%)

Query: 7 LSFMMFVEWFIWGAWFVPLWLWL----SKSGFSAGEIGWSYACTAIAAILSPILVGSITD 62
++ +M V + + VP LW+ + + A IG S A I L+ ++
Sbjct: 212 VAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVA 271

Query: 63 RFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYSLTYMPTIALTNSIA 116
++ L + M A A T FP+++ + + AL ++
Sbjct: 272 ARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLS 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12350TYPE3OMGPROT260.029 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 26.4 bits (58), Expect = 0.029
Identities = 13/42 (30%), Positives = 21/42 (50%), Gaps = 1/42 (2%)

Query: 6 KMLLGVLLLVTSAAWAAPATAGSTNTSGISKYE-LSSFIADF 46
++L G LLL++S +WA ++K E L + DF
Sbjct: 11 RVLTGTLLLLSSYSWAQELDWLPIPYVYVAKGESLRDLLTDF 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12360PF005777130.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 713 bits (1843), Expect = 0.0
Identities = 239/843 (28%), Positives = 389/843 (46%), Gaps = 35/843 (4%)

Query: 2 LRMTPLASAI---VALLLGIEAYAAEETFDTHFMIGGMKDQQVANIRL--DDNQPLPGQY 56
R+ + A +AE F+ F+ Q VA++ + + PG Y
Sbjct: 21 HRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADD--PQAVADLSRFENGQELPPGTY 78

Query: 57 DIDIYVNKQWRGKYEIIVKDNPQET----CLSREVIKRLGIN-----SDNFASGKQCLTF 107
+DIY+N + ++ E CL+R + +G+N N + C+
Sbjct: 79 RVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPL 138

Query: 108 EQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYVSQYYSDY 167
++ + D+G RL+ ++PQA++ GY+PPE W+ GINA +Y S
Sbjct: 139 TSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN 198

Query: 168 KASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGV-----WKSNTLYLERGFAQFL 222
+ GN+ Y+ SGLN+ W+L + ++S +++ W+ +LER
Sbjct: 199 RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLR 258

Query: 223 GTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGF 282
L +GD YT DIFD + F G +L D MLP+S++ F P + GIA+ A VTI+QNG+
Sbjct: 259 SRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGY 318

Query: 283 VVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDF 342
+Y VPPGPF I D+ AG DL V++KEADGS + VPY++VP + + G ++Y
Sbjct: 319 DIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSI 378

Query: 343 AAGRSHIEGASKQSD-FVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNT-RIGAIS 400
AG A ++ F Q+ +G T+YGGT +A+ Y AF G G N +GA+S
Sbjct: 379 TAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALS 438

Query: 401 VDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNK 460
VD T+++S + DGQS + YNK ++++ T L +RYS+ Y F D ++
Sbjct: 439 VDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMN 498

Query: 461 DNYRRDENDIYDI----ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSG 516
++ + + DYY + ++ ++Q L ++ LS + YWG S
Sbjct: 499 GYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGR-TSTLYLSGSHQTYWGTSN 557

Query: 517 SSKDYQLSYSNNWRRISYTLAASQAYGENHHE-EKRFNIFISIPCD--WGDDVTTPRRQI 573
+ +Q + + I++TL+ S ++ + ++IP D + R
Sbjct: 558 VDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHA 617

Query: 574 YMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGN---ETTAGANLTWNAPV 630
S S + D G +N G+ GT+ + +Y V + G+ +T A L +
Sbjct: 618 SASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGY 677

Query: 631 ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYR 690
N YS S +Q VSGG++A + GV L L++T ++ APG KDA V Q
Sbjct: 678 GNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGV 737

Query: 691 TTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKP 750
T+ G V T YREN + LD + +L P RGA+V F +
Sbjct: 738 RTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKA-RVGI 796

Query: 751 WFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCT 810
+ L + +PL FG V + G+V Q+++ + V V +++ C
Sbjct: 797 KLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV 856

Query: 811 ITF 813
+
Sbjct: 857 ANY 859


38B7485_12185B7485_12685Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_12185-2133.595812DedA family protein
B7485_12190-2133.563680multidrug transporter permease
B7485_12195-2184.332822hypothetical protein
B7485_12200-1193.981129tRNA dihydrouridine(16) synthase DusC
B7485_12205-1194.022685hypothetical protein
B7485_12210-1193.505262CidB/LrgB family autolysis modulator
B7485_12215-2152.150955cytidine deaminase
B7485_12220-2110.497342vancomycin high temperature exclusion protein
B7485_12225-112-1.107601hypothetical protein
B7485_12230015-2.495209dihydropyrimidine dehydrogenase
B7485_12235113-2.703598dihydroorotate dehydrogenase
B7485_12240420-3.555009methyl-galactoside ABC transporter permease
B7485_12245421-3.723126galactose/methyl galactoside ABC transporter
B7485_12250422-3.617287galactose ABC transporter substrate-binding
B7485_12255420-3.692672DNA-binding transcriptional regulator GalS
B7485_12260318-2.704294hypothetical protein
B7485_12265215-2.113439DUF418 family protein
B7485_12270013-2.368779GTP cyclohydrolase I FolE
B7485_12275012-1.858256phenylalanyl-tRNA synthetase subunit beta
B7485_122851131.107262hypothetical protein
B7485_122952140.377437catecholate siderophore receptor CirA
B7485_123002171.490773lysine transporter
B7485_123052171.649626LysR family transcriptional regulator
B7485_123101181.101685hypothetical protein
B7485_123150190.246576endonuclease
B7485_123200190.199951kinase
B7485_123253180.983386NupC/NupG family nucleoside CNT transporter
B7485_12330423-4.458118ribonucleoside hydrolase
B7485_12335323-6.470548NupC/NupG family nucleoside CNT transporter
B7485_12345326-8.346916pseudouridine-5'-phosphate glycosidase
B7485_12350328-8.940229pseudouridine kinase
B7485_12355330-9.477592PTS fructose transporter subunit EIIBC
B7485_12360123-6.1133291-phosphofructokinase
B7485_12365-215-2.988311bifunctional PTS fructose transporter subunit
B7485_12370016-1.525324MFS transporter
B7485_12375114-0.620049zinc/iron-chelating domain-containing protein
B7485_12380112-0.863018elongation factor P-like protein YeiP
B7485_12385212-0.245333fructuronate reductase
B7485_12395026-1.293887GTP-binding protein
B7485_12400027-1.724715hypothetical protein
B7485_12405024-1.214620bifunctional murein DD-endopeptidase/murein
B7485_124150210.173461phage resistance protein
B7485_124201180.618314ABC transporter substrate-binding protein
B7485_124302172.261274microcin ABC transporter permease
B7485_124402160.079951microcin ABC transporter permease
B7485_12445215-0.785430microcin C ABC transporter ATP-binding protein
B7485_12455-213-0.961436hypothetical protein
B7485_12465-214-0.921742Bcr/CflA family multidrug efflux MFS
B7485_12470-115-1.95370616S rRNA pseudouridine(516) synthase
B7485_12475321-2.359911ATP-dependent helicase
B7485_12480828-2.47446850S ribosomal protein L25
B7485_12485727-3.649840nucleoid-associated protein
B7485_12490727-1.632052hypothetical protein
B7485_12495526-1.839747sulfatase
B7485_12500523-0.898495*integrase
B7485_125052210.687706hypothetical protein
B7485_12510221-1.012116host cell division inhibitor Icd-like protein
B7485_12515122-1.431499hypothetical protein
B7485_12525225-0.726751hypothetical protein
B7485_12530226-0.377169DNA primase
B7485_125350210.398373hypothetical protein
B7485_12540-1202.930586IS3 family transposase
B7485_125450203.731545hypothetical protein
B7485_125550163.370119IS30 family transposase IS30
B7485_12560-2152.813450hypothetical protein
B7485_12570-2161.924823hypothetical protein
B7485_12575-2161.385389group II intron reverse transcriptase/maturase
B7485_12580-1181.268596IS110 family transposase
B7485_125850151.400284hypothetical protein
B7485_125953212.648056GtrA family protein
B7485_126002232.722690glycosyltransferase
B7485_126052202.307748hypothetical protein
B7485_126102182.246809IS3 family transposase
B7485_126151171.882847proQ/FINO family protein
B7485_126201151.360871PerC family transcriptional regulator
B7485_126252140.160735outer membrane autotransporter barrel
B7485_12630218-0.516152hypothetical protein
B7485_12635316-0.531013DNA-binding response regulator
B7485_12640215-0.995666cytochrome c-type biogenesis protein CcmH
B7485_12645215-1.856674thiol:disulfide interchange protein
B7485_12650417-3.005099c-type cytochrome biogenesis protein CcmF
B7485_12655115-2.426406cytochrome c-type biogenesis protein CcmE
B7485_12660014-2.326910heme exporter protein D
B7485_12665014-2.546848heme exporter protein C
B7485_12670-113-1.842762heme exporter protein B
B7485_12675-111-1.754685cytochrome c biogenesis ATP-binding export
B7485_126801110.003070cytochrome c-type protein NapC
B7485_12685214-0.124242periplasmic nitrate reductase electron transfer
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12600BCTERIALGSPF280.019 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 28.3 bits (63), Expect = 0.019
Identities = 5/33 (15%), Positives = 16/33 (48%), Gaps = 2/33 (6%)

Query: 152 WLHNLDQHLKHW-VWLILVVVL-VVGVRWWLKR 182
L + ++ + W++L ++ + R L++
Sbjct: 215 VLMGMSDAVRTFGPWMLLALLAGFMAFRVMLRQ 247


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12625SHAPEPROTEIN290.017 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 29.3 bits (66), Expect = 0.017
Identities = 32/127 (25%), Positives = 53/127 (41%), Gaps = 5/127 (3%)

Query: 122 GAKAMREAVPAHLPVSVKVRLGWDSGEK-KFEIADAVQQAGATELVVHGRTKEQGY-RAE 179
G EA+ ++ + +G + E+ K EI A E+ V GR +G R
Sbjct: 190 GGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGF 249

Query: 180 HIDWQAIGE-IRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVK 238
++ I E +++ L V A + + IS V+ G GAL + NL R++
Sbjct: 250 TLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL-LRNLDRLL- 307

Query: 239 YNEPRMP 245
E +P
Sbjct: 308 MEETGIP 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12675PF05272320.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 32.0 bits (72), Expect = 0.006
Identities = 21/74 (28%), Positives = 28/74 (37%), Gaps = 17/74 (22%)

Query: 24 PGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAK 83
PG K D + L G G GKSTL+ L G+ F D + K
Sbjct: 591 PGCKF-DYSVV---------LEGTGGIGKSTLINTLVGLD-------FFSDTHFDIGTGK 633

Query: 84 EALENGISMVHQEL 97
++ E +V EL
Sbjct: 634 DSYEQIAGIVAYEL 647


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12685ACETATEKNASE310.008 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 30.5 bits (69), Expect = 0.008
Identities = 18/77 (23%), Positives = 27/77 (35%), Gaps = 8/77 (10%)

Query: 4 IRDVARQAGVSVATVSRVLNNS------TLVSADTREAVMKAVSELDYRPNANAQALATQ 57
I + + +S V +LN + +S+D R+ A D R A +
Sbjct: 249 ISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRAQLALNVFAYR 308

Query: 58 VSDTIG--VVVMDVSDA 72
V TIG M D
Sbjct: 309 VKKTIGSYAAAMGGVDV 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12805TCRTETA508e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 49.8 bits (119), Expect = 8e-09
Identities = 74/381 (19%), Positives = 118/381 (30%), Gaps = 24/381 (6%)

Query: 21 LIVAFLTGIAGALQTPTLSIFLTDEVHA--RPAMVGFFFTGSAVIGILVSQFLAGRSDKR 78
L L + L P L L D VH+ A G A++ + L SD+
Sbjct: 11 LSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRF 70

Query: 79 GDRKSLIVFCCLLGVLACTLFAWNRNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTG 138
G R+ +++ + + A +VL ++G ++ G T A A G
Sbjct: 71 G-RRPVLLVSLAGAAVDYAIMATAPFLWVL-YIGRIVA--GITGATGAVAGAYIADITDG 126

Query: 139 REAVMFSSFLRAQVSLAWVIGPPLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQ 198
E F+ A V GP L L GFS + +AA + + LP
Sbjct: 127 DERARHFGFMSACFGFGMVAGPVLG-GLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESH 185

Query: 199 K-------ELPLATGTVEAPRRNRRDTLLLFVICTLMWGSNSLYIINMPLFIINELHLPE 251
K L R L + +M + +F + H
Sbjct: 186 KGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDA 245

Query: 252 KLAGVMMGTAAGLEIPT-MLIAGYFAKRLGKRFLMRVAAVGGVCFYAGMLMA-HSPAILL 309
G+ + L +I G A RLG+R + + + Y + A
Sbjct: 246 TTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFP 305

Query: 310 GLQLLNAIFIGILGGIGMLYFQDLMPGQAGSATTLYTNTSRVGWIIAGSVAG--IVAEIW 367
+ LL GGIGM Q ++ Q S S+ G + I+
Sbjct: 306 IMVLL------ASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIY 359

Query: 368 NYHAVFWFAMVMIIATLFWVM 388
W I +++
Sbjct: 360 AASITTWNGWAWIAGAALYLL 380



Score = 40.2 bits (94), Expect = 1e-05
Identities = 18/101 (17%), Positives = 35/101 (34%)

Query: 19 AFLIVAFLTGIAGALQTPTLSIFLTDEVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKR 78
A + V F+ + G + IF D H +G ++ L + G R
Sbjct: 214 ALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAAR 273

Query: 79 GDRKSLIVFCCLLGVLACTLFAWNRNYFVLLFVGVFLSSFG 119
+ ++ + L A+ ++ + V L+S G
Sbjct: 274 LGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGG 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12880TCRTETB621e-12 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 61.8 bits (150), Expect = 1e-12
Identities = 79/408 (19%), Positives = 152/408 (37%), Gaps = 59/408 (14%)

Query: 11 IVFILGLLAMLMPLSIDMYLPALPVISAQFGVSAGSTQMTLSTYILGFALGQLIYGPMAD 70
I+ L +L+ L+ + +LP I+ F ST + ++L F++G +YG ++D
Sbjct: 15 ILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD 74

Query: 71 SFGRKPVVLGGTLVFAAAAVACALAQTIDQLIVM-RFFHGLAAAAASVVINALMRDIYPK 129
G K ++L G ++ +V + + L++M RF G AAA ++ ++ PK
Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK 134

Query: 130 EEFSRMMSFVMLVTTIALLMAPIVGGWVLVWLSWHYIFWILALAAILASAMIFFLIKETL 189
E + + + + + P +GG + ++ W Y+ I + I + FL+K
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITII----TVPFLMKLLK 190

Query: 190 PPERR-QPFHIRTTIGNFAA---------------------------------------- 208
R F I+ I
Sbjct: 191 KEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDP 250

Query: 209 -LFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFAL-NIVFLFVMT 266
L ++ + +L G F + F+S P++ +++ ++ G + + +
Sbjct: 251 GLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFG 310

Query: 267 IFNSRFVRRIGALNMFRSG---LWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMV 323
V R G L + G L + F+ A++++ + + ++ V G
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSW----FMTIIIVFVLGGLSFTK 366

Query: 324 SSNAMAVILDEFPHMAGTASSLAGTFRF---GIG-AIVGALLSLATFN 367
+ + V AG SL F G G AIVG LLS+ +
Sbjct: 367 TVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLD 414


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12915IGASERPTASE300.025 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.4 bits (68), Expect = 0.025
Identities = 19/70 (27%), Positives = 28/70 (40%), Gaps = 6/70 (8%)

Query: 503 LHVSTPASEYSQGQ-DLF---NPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNL 558
L V+ E + + LF QR H V+ +T+ + K L N NG+Y YN
Sbjct: 926 LQVADKTGEPNHNELTLFDASKAQRDHLNVSLV-GNTVDLGAWKYKLR-NVNGRYDLYNP 983

Query: 559 RGERVKDEKP 568
E+
Sbjct: 984 EVEKRNQTVD 993


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13055PRTACTNFAMLY2044e-57 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 204 bits (520), Expect = 4e-57
Identities = 139/526 (26%), Positives = 220/526 (41%), Gaps = 59/526 (11%)

Query: 350 APAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMS 409
P +G + V+ + R GA + +S++N+ W + + N+ L ++
Sbjct: 405 IPGTSIGPLDVALASQARWTGATRAVDS-LSIDNATWVMTDNS---------NVGALRLA 454

Query: 410 GANVIMMAEPVTRSSVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGD 469
+ +P A A F LT NTL+G+G F M SD+L V A+G
Sbjct: 455 SDGSVDFQQP-------AEAGRFKVLTVNTLAGSGLFRMNVFADLGLSDKLVVMQDASGQ 507

Query: 470 FKIFVTDTGASPAAGDSLTLVTT-GGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLA 528
+++V ++G+ PA+ ++L LV T G A FTL N G VDIGTY Y L NGN WSL
Sbjct: 508 HRLWVRNSGSEPASANTLLLVQTPLGSAATFTLANKDGKVDIGTYRYRLAANGNGQWSLV 567

Query: 529 ENRAQITPSTTDVLNMAAAQPL-----------------------------------VFD 553
+A P QP ++
Sbjct: 568 GAKAPPAPKPAPQPGPQPPQPPQPQPEAPAPQPPAGRELSAAANAAVNTGGVGLASTLWY 627

Query: 554 AELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREE 613
AE + + +RLG ++ W R + AG F+Q + G LG D +
Sbjct: 628 AESNALSKRLGELRLNPDAGGAWGRGFAQRQQLDNRAGRRFDQKVAGFELGADHAVAVAG 687

Query: 614 SSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHG 673
G GY+ D GF G G+ DS +G YA + +G Y+D ++ R N
Sbjct: 688 GRWHLGGLAGYTRGDRGFTGDGGGHTDSVHVGGYATYIADSGFYLDATLRASRLENDFKV 747

Query: 674 KMSNGATAFGDYNSNGAGAHVESGFRW-VDGLWSVRPYLAFTGFTTDGQDYTLSNGMR-- 730
S+G G Y ++G GA +E+G R+ W + P F G Y +NG+R
Sbjct: 748 AGSDGYAVKGKYRTHGVGASLEAGRRFTHADGWFLEPQAELAVFRAGGGAYRAANGLRVR 807

Query: 731 ADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNNDVA 790
+ G++ + R G V ++L G ++P++KA+V QE+ + V N ++
Sbjct: 808 DEGGSSVLGR--LGLEVGKRIELAGGRQVQPYIKASVLQEFDGAGTVHTNGIAH-RTELR 864

Query: 791 GTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF 836
GT G+ ++ S + S Y G + PW AG +++
Sbjct: 865 GTRAELGLGMAAALGRGHSLYASYEYSKGPKLAMPWTFHAGYRYSW 910


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13065HTHFIS637e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.9 bits (153), Expect = 7e-14
Identities = 22/114 (19%), Positives = 46/114 (40%), Gaps = 2/114 (1%)

Query: 9 VMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASAIDLANRLDIDVILLDLNMKGMSGL 68
+++ DD +R + Q L G++V A+ D D+++ D+ M +
Sbjct: 6 ILVADDDAAIRTVLNQALS-RAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 69 DTLNALRRDGVTAQIIILTVSDASSDVFALIDAGADGYLLKDSDPEVLLEAIRT 122
D L +++ +++++ + + GA YL K D L+ I
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


39B7485_12955B7485_13125Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_12955332-4.141738hypothetical protein
B7485_12960433-4.885627nucleoside triphosphatase
B7485_12965634-5.026573histidine phosphatase family protein
B7485_12970734-3.682082UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate
B7485_12975635-2.321444undecaprenyl-phosphate
B7485_12980532-0.206726bifunctional UDP-glucuronic acid
B7485_129856302.0971324-deoxy-4-formamido-L-arabinose-
B7485_129952280.5659644-amino-4-deoxy-L-arabinose lipid A transferase
B7485_13000126-0.4422224-amino-4-deoxy-L-arabinose-phospho-UDP
B7485_13005025-3.1946534-amino-4-deoxy-L-arabinose-phospho-UDP
B7485_13015028-2.960059signal transduction protein PmrD
B7485_13020026-2.3801592-succinylbenzoate-CoA ligase
B7485_13030221-2.258289o-succinylbenzoate synthase
B7485_13040117-0.4193421,4-dihydroxy-2-naphthoyl-CoA synthase
B7485_13045014-1.5059872-succinyl-6-hydroxy-2,
B7485_13055-1140.4374732-succinyl-5-enolpyruvyl-6-hydroxy-3-
B7485_130650213.483008isochorismate synthase MenF
B7485_130700223.909363protein ElaB
B7485_130751224.176437protein ElaA
B7485_130850162.991994ribonuclease Z
B7485_130901173.187786hypothetical protein
B7485_130950163.298886NADH-quinone oxidoreductase subunit N
B7485_13100-1173.892259NADH-quinone oxidoreductase subunit M
B7485_13105-1214.035031NADH-quinone oxidoreductase subunit L
B7485_13110-1233.997863NADH-quinone oxidoreductase subunit K
B7485_13115-1214.364335NADH-quinone oxidoreductase subunit J
B7485_13120-1203.882682NADH-quinone oxidoreductase subunit I
B7485_13125-1203.205380NADH-quinone oxidoreductase subunit H
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13380TYPE3IMSPROT290.037 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 28.6 bits (64), Expect = 0.037
Identities = 14/61 (22%), Positives = 28/61 (45%), Gaps = 3/61 (4%)

Query: 97 PVMVDVDRDTLMVT-PEAIESAIT-PRTKAIIP-VHYAGAPADIDAIRAIGERYGIAVIE 153
+ +V R +++V P I I R + +P V + A + +R I E G+ +++
Sbjct: 249 NMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQ 308

Query: 154 D 154

Sbjct: 309 R 309


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13390NUCEPIMERASE1144e-30 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 114 bits (288), Expect = 4e-30
Identities = 73/361 (20%), Positives = 136/361 (37%), Gaps = 60/361 (16%)

Query: 317 RVLILGVNGFIGNHLTERLLREDHYEVYGLDIGSD--------AISRFLNHPHFHFVEGD 368
+ L+ G GFIG H+++RLL H +V G+D +D A L P F F + D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGH-QVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 369 ISIHSEWIE--YHVKKCDVVLPLVAIATPIEYT-RNPLRVFELDFEENLRIIRYCVKYR- 424
++ E + + + V + Y+ NP + + L I+ C +
Sbjct: 61 LADR-EGMTDLFASGHFERVFISPHRLA-VRYSLENPHAYADSNLTGFLNILEGCRHNKI 118

Query: 425 KRIIFPSTSEVYGMCSDKYFDEDHSNLIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQ 484
+ +++ S+S VYG+ F D V+ P +Y+ +K+ + + Y GL
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDD------SVDHPVSLYAATKKANELMAHTYSHLYGLP 172

Query: 485 FTLFLPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGI 544
T F GP A+ + ++EG I + + GK KR FT I D
Sbjct: 173 ATGLRFFTVYGPWGR--------PDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIA 224

Query: 545 EALYRIIEN---------------AGNRCDGEIINIGNPENEASIEELGEMLLASFEKHP 589
EA+ R+ + A + + NIGN + + + L +
Sbjct: 225 EAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVE-LMDYIQALEDALGIEA 283

Query: 590 LRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIRNAHRCLDWEPKIDMQETIDETLDFF 649
++ P G DV + + + + P+ +++ + ++++
Sbjct: 284 KKNMLPLQPG---------------DVLETSADTKALYEVIGFTPETTVKDGVKNFVNWY 328

Query: 650 L 650

Sbjct: 329 R 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13405BCTERIALGSPC322e-04 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 31.9 bits (72), Expect = 2e-04
Identities = 13/38 (34%), Positives = 19/38 (50%), Gaps = 1/38 (2%)

Query: 34 KHIVLWLGLALACLGLAMMLWLLVL-QNVPVSPRHWCG 70
+ I+ +L + L C LAM+ W + L N PVS
Sbjct: 15 RRILFYLLMLLFCQQLAMIFWRIGLPDNAPVSSVQITP 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13455AUTOINDCRSYN356e-05 Autoinducer synthesis protein signature.
		>AUTOINDCRSYN#Autoinducer synthesis protein signature.

Length = 216

Score = 34.8 bits (80), Expect = 6e-05
Identities = 14/79 (17%), Positives = 32/79 (40%), Gaps = 12/79 (15%)

Query: 1 MIEWQDLHHSELSVSQLYALLQLRCAVFV--------VEQNCPYQDIDGDDLTGDNRHIL 52
M+E D++H+ LS ++ L LR F + D + + ++
Sbjct: 1 MLEIFDVNHTLLSETKSGELFTLRKETFKDRLNWAVQCTDGMEFDQYDNN----NTTYLF 56

Query: 53 GWKNDELVAYARILKSDDD 71
G K++ ++ R +++
Sbjct: 57 GIKDNTVICSLRFIETKYP 75


40B7485_13365B7485_13525Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_13365218-1.676461acetyl-CoA carboxylase carboxyl transferase
B7485_13375117-1.212951hypothetical protein
B7485_13385015-0.749731tRNA pseudouridine(38-40) synthase TruA
B7485_133951150.752878aspartate-semialdehyde dehydrogenase
B7485_134050153.413626erythronate-4-phosphate dehydrogenase
B7485_134151154.849611flagella biosynthesis regulator
B7485_134201154.639180MFS transporter
B7485_134250144.444612hypothetical protein
B7485_134350142.412811beta-ketoacyl-[acyl-carrier-protein] synthase I
B7485_13445-120-0.818644bifunctional tRNA
B7485_13455029-4.238259hypothetical protein
B7485_13460030-4.741225elongation factor P hydroxylase
B7485_13465-116-1.328906hypothetical protein
B7485_134750222.129859penicillin-insensitive murein endopeptidase
B7485_134851283.605806chorismate synthase
B7485_134900304.09788150S ribosomal protein L3 N(5)-glutamine
B7485_134950313.494682endonuclease SmrB
B7485_135000313.957003fimbrial protein
B7485_135100303.785618fimbrial protein
B7485_135151293.954070fimbrial protein
B7485_135200283.767991fimbrial protein
B7485_135251273.791176type 1 fimbrial protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13705FbpA_PF05833280.039 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 28.3 bits (63), Expect = 0.039
Identities = 21/63 (33%), Positives = 31/63 (49%), Gaps = 6/63 (9%)

Query: 204 VRNIVGSLMEV-GAHNQPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPP 262
+NI GS + V + PES + E AA LAA +K++ V VDY + ++ KP
Sbjct: 496 TKNIPGSHVIVKNIMDIPESTLLE--AAN---LAAYYSKSQNSSNVPVDYTEVKNVKKPN 550

Query: 263 MGP 265

Sbjct: 551 GAK 553


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13725TCRTETA432e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 42.5 bits (100), Expect = 2e-06
Identities = 80/366 (21%), Positives = 132/366 (36%), Gaps = 42/366 (11%)

Query: 16 SLFRISFAVFLTYMTVGLPLPVIPLFVHHDLGYGNTM--VGIAVGIQFLATVLTRGYAGR 73
L I V L + +GL +PV+P + + + GI + + L G
Sbjct: 6 PLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGA 65

Query: 74 LADQYGAKRSALQGMLACGLAGGAL--LLAAILPVSAPFKFALLVIGRLILGFGESQLLT 131
L+D++G + +L LAG A+ + A P +L IGR++ G +T
Sbjct: 66 LSDRFGRRP-----VLLVSLAGAAVDYAIMATAPF-----LWVLYIGRIVAG------IT 109

Query: 132 GALTWGLG-----IVGPKHSGKVMSWNGMAIYGALAVGAPLGLL---IHSHYGF---AAL 180
GA G I + + + G LG L H F AAL
Sbjct: 110 GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAAL 169

Query: 181 AITT--MVLPLLAWACNGTVRKVPALAGERPSLWSVV----GLIWKPGLGLALQGVGFAV 234
LL + G R + A + + + + +Q VG
Sbjct: 170 NGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVP 229

Query: 235 IGTFVSLYFASKGW--AMAGFTLTAFGGAFVVMRVM-FGWMPDRFGGVKVAIVSLLVETV 291
+V W G +L AFG + + M G + R G + ++ ++ +
Sbjct: 230 AALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGT 289

Query: 292 GLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQVRGTALGGYAAFQDIAL 351
G +LL A W+A L +G + PAL + ++V + +G G AA +
Sbjct: 290 GYILLAFATRGWMAFPIMVLLASG-GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLT- 347

Query: 352 GVSGPL 357
+ GPL
Sbjct: 348 SIVGPL 353



Score = 31.7 bits (72), Expect = 0.005
Identities = 35/142 (24%), Positives = 49/142 (34%), Gaps = 8/142 (5%)

Query: 252 GFTLTAFGGAFVVMRVMFGWMPDRFGGVKVAIVSLLVETVGLLLLWQAPGAWVALAG--- 308
G L + + G + DRFG V +VSL V ++ AP WV G
Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIV 105

Query: 309 AALTGAGCSLIFPALGVEVVKRVPSQVRGTALGGYAAFQDIALGVSGPLAGMLATTFGYS 368
A +TGA G + R G +A + V+GP+ G L F
Sbjct: 106 AGITGA----TGAVAGAYIADITDGDERARHFGFMSACFGFGM-VAGPVLGGLMGGFSPH 160

Query: 369 SVFLAGAISAVLGIIVTILSFR 390
+ F A A L +
Sbjct: 161 APFFAAAALNGLNFLTGCFLLP 182


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13785FIMBRIALPAPE334e-04 Escherichia coli: P pili tip fibrillum papE protein...
		>FIMBRIALPAPE#Escherichia coli: P pili tip fibrillum papE protein

signature.
Length = 173

Score = 32.7 bits (74), Expect = 4e-04
Identities = 49/182 (26%), Positives = 75/182 (41%), Gaps = 19/182 (10%)

Query: 1 MKKKRTLFFISSL-MLLGSGTTIAGDNLHFTGNLISKSCTPVINGSQLAEVHFPAIAASD 59
MKK R L L +L S A DNL F G LI +CT Q AEV++ I +
Sbjct: 1 MKKIRGLCLPVMLGAVLMSQHVHAADNLTFKGKLIIPACT-----VQNAEVNWGDIEIQN 55

Query: 60 LMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFLAFDSSSSASGAGIGIET 119
L+ G +++ + +S V +T G ++ + ++S+ASG G+ I
Sbjct: 56 LVQSGGNQK-DFTVDMNCPYSLGTMKVTITSNGQTGNS----ILVPNTSTASGDGLLIYL 110

Query: 120 AAGTSVPINNTTGVTLPLNQGN---NSLNFNTWLQAKSG-----RDVTSGDFSATVTATF 171
+ I N + + G + L AK G + + +G FSAT T
Sbjct: 111 YNSNNSGIGNAVTLGSQVTPGKITGTAPARKITLYAKLGYKGNMQSLQAGTFSATATLVA 170

Query: 172 EY 173
Y
Sbjct: 171 SY 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13790FIMBRIALPAPF438e-08 Escherichia coli: P pili tip fibrillum papF protein...
		>FIMBRIALPAPF#Escherichia coli: P pili tip fibrillum papF protein

signature.
Length = 167

Score = 42.8 bits (100), Expect = 8e-08
Identities = 44/171 (25%), Positives = 74/171 (43%), Gaps = 21/171 (12%)

Query: 1 MKRISL---ILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITFQDVLIDDINGSNY 57
M R+SL +LL +A ++ G + PP CTI+N Q I + F ++ + ++ S
Sbjct: 1 MIRLSLFISLLLTSVAVLADVQINIRGNVYIPP-CTINNGQNIVVDFGNINPEHVDNSRG 59

Query: 58 EQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLGIQLKQ---------- 107
E T SI+C +++T + G N +++NIT GI L Q
Sbjct: 60 EVTKNISISCPYKSGSLWIKVTGNTMGVGQ---NNVLATNITHFGIALYQGKGMSTPLTL 116

Query: 108 ---AGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDY 155
+G + + L + T+VP + IL DF A++ + Y
Sbjct: 117 GNGSGNGYRVTAGLDTARSTF-TFTSVPFRNGSGILNGGDFRTTASMSMIY 166


41B7485_13770B7485_13950Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_13770021-3.637914divalent metal cation transporter
B7485_13775127-4.697444NupC/NupG family nucleoside CNT transporter
B7485_13780228-3.457602histidine kinase
B7485_13785223-2.955284**hypothetical protein
B7485_13790222-2.141376hypothetical protein
B7485_13800-217-0.835596glutamate--tRNA ligase
B7485_13805-2160.188225***flxA-like family protein
B7485_13810-2120.650306LysR family transcriptional regulator
B7485_13815-2130.721496bile acid:sodium symporter
B7485_13820-114-2.154659hypothetical protein
B7485_13825016-2.738000DNA ligase (NAD(+)) LigA
B7485_13830017-3.512095cell division protein ZipA
B7485_13835221-6.168981sulfate transporter CysZ
B7485_13840119-6.001151cysteine synthase A
B7485_13845221-7.498055phosphocarrier protein HPr
B7485_13850-119-2.269662phosphoenolpyruvate--protein phosphotransferase
B7485_13855019-0.855702glucose-specific phosphotransferase enzyme IIA
B7485_13860119-1.036444pyridoxine kinase
B7485_13865219-0.795299hypothetical protein
B7485_13875221-0.458892cysteine synthase B
B7485_13880218-0.212464sulfate/thiosulfate import ATP-binding protein
B7485_13885121-2.804065sulfate ABC transporter permease
B7485_13890026-4.676188sulfate ABC transporter permease subunit CysT
B7485_13895029-6.345504thiosulfate transporter subunit
B7485_13905032-8.857682NAD(P)-dependent oxidoreductase
B7485_13910034-9.769615N-acetylmuramic acid 6-phosphate etherase
B7485_13915034-8.902353peroxidase
B7485_13920132-8.159556RpoE-regulated lipoprotein
B7485_13930232-6.120413hypothetical protein
B7485_13940127-4.694261acetyltransferase YpeA
B7485_13945-122-4.560604N-acetylmuramoyl-L-alanine amidase
B7485_13950-122-3.576545coproporphyrinogen III oxidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14130PF03544473e-08 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 46.9 bits (111), Expect = 3e-08
Identities = 28/123 (22%), Positives = 42/123 (34%), Gaps = 1/123 (0%)

Query: 68 VHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPRQPVQQPPEAQVPPQHAPRPAQPVQQ 127
VH+V PA AQ +P P P V+ PE + P P+ A V +
Sbjct: 37 VHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIP-EPPKEAPVVIE 95

Query: 128 PAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDK 187
+P Q +PV S P + PA P ++ ++P +
Sbjct: 96 KPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVAS 155

Query: 188 PKR 190
R
Sbjct: 156 GPR 158



Score = 29.6 bits (66), Expect = 0.017
Identities = 21/79 (26%), Positives = 27/79 (34%), Gaps = 1/79 (1%)

Query: 125 VQQPAYQPQPEQPLQ-QPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAP 183
V Q P P QP+ V+P PQ V P+P + EP+ P E
Sbjct: 37 VHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEK 96

Query: 184 VMDKPKRKEAVIIMNVAAH 202
KPK K +
Sbjct: 97 PKPKPKPKPKPVKKVEQPK 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14150PHPHTRNFRASE7480.0 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 748 bits (1933), Expect = 0.0
Identities = 276/571 (48%), Positives = 386/571 (67%), Gaps = 2/571 (0%)

Query: 1 MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFLSGRAKASAQLETIKTK 60
I+GI AS G+A KA + E + I++ I V E+E+ + K+ +L IK +
Sbjct: 4 KITGIAASSGVAIAKAFIHLEPNVDIEKTSI--TDVSTEIEKLTAALEKSKEELRAIKDQ 61

Query: 61 AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQASALEELDD 120
+ G +K IF H+++L+D EL I I+++ M A+ A EV + S E +D+
Sbjct: 62 TEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDN 121

Query: 121 EYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVAADLTPSETAQLNLKKVLGFI 180
EY+KERAAD+RD+ KR+L +++G++ L+ I +E +++A DLTPS+TAQLN + V GF
Sbjct: 122 EYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFA 181

Query: 181 TDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYLILDAVNNQVYVNPTNEVIDKMR 240
TD GGRTSH++IM+RSLE+PA+VGT VT ++++ D +I+D + V VNPT E +
Sbjct: 182 TDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYE 241

Query: 241 AVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIGTVRDVEGAERNGAEGVGLYRTEFL 300
+ +K E AKL P+ T DG VE+ ANIGT +DV+G NG EG+GLYRTEFL
Sbjct: 242 EKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFL 301

Query: 301 FMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTMDIGGDKELPYMNFPKEENPFLGWRAI 360
+MDRD LPTEEEQF AYK V + + V++RT+DIGGDKEL Y+ PKE NPFLG+RAI
Sbjct: 302 YMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFRAI 361

Query: 361 RIAMDRKEILRDQLRAILRASAFGKLRIMFPMIISVEEVRALRKEIEIYKQELRDEGKAF 420
R+ +++++I R QLRA+LRAS +G L++MFPMI ++EE+R + ++ K +L EG
Sbjct: 362 RLCLEKQDIFRTQLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDV 421

Query: 421 DESIEIGVMVETPAAATIARHLAKEVDFFSIGTNDLTQYTLAVDRGNDMISHLYQPMSPS 480
+SIE+G+MVE P+ A A AKEVDFFSIGTNDL QYT+A DR N+ +S+LYQP P+
Sbjct: 422 SDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPA 481

Query: 481 VLNLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT 540
+L L+ VI A+H+EGKW GMCGE+AGDE A LLLG+GLDEFSMSA SI + +
Sbjct: 482 ILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKL 541

Query: 541 NFEDAKVLAEQALAQPTTDELMTLVNKFIEE 571
+ E+ K A++AL T +E+ LV K +
Sbjct: 542 SKEELKPFAQKALMLDTAEEVEQLVKKTYLK 572


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14180PF05272357e-04 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 34.7 bits (79), Expect = 7e-04
Identities = 11/33 (33%), Positives = 16/33 (48%)

Query: 30 MVALLGPSGSGKTTLLRIIAGLEHQTSGHIRFH 62
V L G G GK+TL+ + GL+ + H
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIG 630


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14205DHBDHDRGNASE1531e-47 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 153 bits (387), Expect = 1e-47
Identities = 96/255 (37%), Positives = 136/255 (53%), Gaps = 4/255 (1%)

Query: 4 LTGKTALITGALQGIGEGIARTFARHGANLILLDISPE-IEKLADELCGRGHRCTAVVAD 62
+ GK A ITGA QGIGE +ART A GA++ +D +PE +EK+ L A AD
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 63 VRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMSDEDRDFHIDINIKGVWNVTK 122
VRD A++ R + + G IDILVN AGV R G +SDE+ + +N GV+N ++
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 123 AVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKAAIVGLTKSLAVEYAQSGIRVNA 182
+V M+ R+ G IV + S V AYA +KAA V TK L +E A+ IR N
Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAG-VPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184

Query: 183 ICPGYVRTPMAESIARQSNPEDP--ESVLTEMAKAIPLCRLADPLEVGELAAFLASDESS 240
+ PG T M S+ N + + L IPL +LA P ++ + FL S ++
Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAG 244

Query: 241 YLTGTQNVIDGGSTL 255
++T +DGG+TL
Sbjct: 245 HITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14240SACTRNSFRASE316e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 31.5 bits (71), Expect = 6e-04
Identities = 15/102 (14%), Positives = 38/102 (37%), Gaps = 4/102 (3%)

Query: 24 LRPWNDPEMDIERKMNHDVSLFLVAEVNGEVVG--TVMGGYDGHRGSAYYLGVHPEFRGR 81
+ + D +MD+ + FL + +G + ++G + V ++R +
Sbjct: 47 FKQYEDDDMDVSYVEEEGKAAFL-YYLENNCIGRIKIRSNWNG-YALIEDIAVAKDYRKK 104

Query: 82 GIANALLNRLEKKLIARGCPKIQINVPEDNDMVLGMYERLGY 123
G+ ALL++ + + + + N Y + +
Sbjct: 105 GVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


42B7485_14570B7485_14615Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_14570-212-3.563778ABC transporter permease
B7485_14575-117-3.705167ABC transporter ATP-binding protein
B7485_14585126-5.937876sugar ABC transporter substrate-binding protein
B7485_14590222-6.936782DUF5107 domain-containing protein
B7485_14595119-2.359333ROK family protein
B7485_14600229-0.100747serine hydroxymethyltransferase
B7485_146052220.387785flavohemoprotein
B7485_146102220.826906nitrogen regulatory protein P-II 1
B7485_146152221.157062two-component system response regulator GlrR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14830FLGFLIH320.002 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 32.1 bits (72), Expect = 0.002
Identities = 28/104 (26%), Positives = 46/104 (44%), Gaps = 13/104 (12%)

Query: 41 QGYYAGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENG 100
QGY G+ QG++ ++ Q I Q +S+ T +D L D++I
Sbjct: 70 QGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDAL-----DSVI-------- 116

Query: 101 SSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGK 144
+SR ++ A EA VI ++ + K + L +PL GK
Sbjct: 117 ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14835SALSPVBPROT350.002 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 34.7 bits (79), Expect = 0.002
Identities = 26/66 (39%), Positives = 34/66 (51%), Gaps = 13/66 (19%)

Query: 99 MTGFTLRPDRAALEIASRVYNGNATPRH--FLWW-ANPAVKGGEGHQSVFPPDVTAVFDH 155
+ G DR+A+ S+V GNATP +LW A PAV Q +F T VFD+
Sbjct: 218 LNGNEAGRDRSAMRYLSKVQYGNATPAADLYLWTSATPAV------QWLF----TLVFDY 267

Query: 156 GKRAVS 161
G+R V
Sbjct: 268 GERGVD 273


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_14860HTHFIS472e-167 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 472 bits (1217), Expect = e-167
Identities = 164/480 (34%), Positives = 248/480 (51%), Gaps = 42/480 (8%)

Query: 6 AHLLLVDDDLGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGM 65
A +L+ DDD + +L L+ GY V + A R + DLV++D+ M + +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 66 QLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALE--- 122
L I+K +P +PV++++A + A+ A+++G + +L KP D L I AL
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 123 --QSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA 180
S D + +V RS M + + Q+D++++I G+SGTGKE+ A+A+H+
Sbjct: 124 RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY 183

Query: 181 SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM 240
R + PF+AIN A+P L+ESELFGH +GAFTGA + G F+ AEGGTLFLDEIGDM
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 241 PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLSKAMARGEFREDLYYRLNVV 300
P Q +LLRVLQ+ + +G I +VRI++AT++DL +++ +G FREDLYYRLNVV
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 301 SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI 360
L++P L +R EDIP L H ++QA + V+ F +A++ + WPGNVR+L N++
Sbjct: 304 PLRLPPLRDRAEDIPDLVRHFVQQAEKEGLD-VKRFDQEALELMKAHPWPGNVRELENLV 362

Query: 361 EQCVALTSSPVISDALVEQALEGENTALPT------------------------------ 390
+ AL VI+ ++E L E P
Sbjct: 363 RRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDA 422

Query: 391 ------FVEARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE 444
+ E + L T+GN AA + G NR K + +
Sbjct: 423 LPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSSR 482


43B7485_14665B7485_14730Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_14665-2153.520575invasion protein
B7485_14670-1173.515851phage tail protein
B7485_14675-1182.838914tail fiber assembly protein
B7485_14680-2123.354861phage tail protein
B7485_146900132.314023phage tail protein
B7485_14695-1172.741719DUF2612 domain-containing protein
B7485_147050213.563426hypothetical protein
B7485_147102222.639785transposase
B7485_147202262.570507ferredoxin
B7485_147251261.032195small toxic protein ShoB
B7485_147302291.356517hypothetical protein
44B7485_14895B7485_14945Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_14895325-2.747916alpha-ketoglutarate permease
B7485_14900529-2.037118*outer membrane protein assembly factor BamD
B7485_14905528-1.810043ribosomal large subunit pseudouridine synthase
B7485_14910426-2.043852laccase domain-containing protein
B7485_149151240.548176chaperone protein ClpB
B7485_149201220.066128hypothetical protein
B7485_14925223-0.324894ribosome-associated inhibitor A
B7485_14930224-0.881012P-protein
B7485_14935224-0.844635bifunctional chorismate mutase/prephenate
B7485_14940224-0.2932473-deoxy-7-phosphoheptulonate synthase
B7485_14945222-1.278999hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15175HTHFIS512e-08 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 50.6 bits (121), Expect = 2e-08
Identities = 51/267 (19%), Positives = 95/267 (35%), Gaps = 52/267 (19%)

Query: 552 MESEREKLLRMEQELHHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGK 611
R L + + ++G++ A+ + + R L + + + G +G GK
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLAR----LMQTDLTL---MITGESGTGK 173

Query: 612 TELCKALANFMFDSDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPY 671
+ +AL ++ + V I+M+ S L G+E+G + T A R
Sbjct: 174 ELVARALHDYGKRRNGPFVAINMAAIPRDLIESEL-------FGHEKGAF-TGAQTRSTG 225

Query: 672 SV-------ILLDEVEKAHPDVFNILLQVLDDG---RLTDGQGRTVDFRNTVVIMTSNLG 721
+ LDE+ D LL+VL G + D R ++ +N
Sbjct: 226 RFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN-- 280

Query: 722 SDLIQERFGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHP-LGE--QHIASIAQIQ 778
DL Q + FR + R++ V + P L + + I + +
Sbjct: 281 KDLKQS----------------INQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHF 324

Query: 779 LKRLYKRLEERGYEIHISDEALKLLSE 805
+++ K E EAL+L+
Sbjct: 325 VQQAEK---EGLDVKRFDQEALELMKA 348



Score = 40.6 bits (95), Expect = 3e-05
Identities = 46/225 (20%), Positives = 76/225 (33%), Gaps = 43/225 (19%)

Query: 112 VLAALESRGTLADILKATGATTANITQAIEQMRGGES-------VNDQGAEDQRQALKKY 164
+L ++ +L + T AI+ G + +AL +
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNT--FMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 165 TIDLTERAEQG-KLDPVIGRDEEIRRTIQVLQRRTKNN-PVLI-GEPGVGKTAIVEGLAQ 221
++ + P++GR ++ +VL R + + ++I GE G GK + L
Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHD 182

Query: 222 R----------IINGEVPEGLKGRRVLALDMGALV-AGAKYRGEFEERLKGVLNDLAKQE 270
I +P L + + GA A + G FE+ G
Sbjct: 183 YGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGT-------- 234

Query: 271 GNVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATT 315
LF+DE+ M MDA L L +GE VG T
Sbjct: 235 ----LFLDEIGDM--------PMDAQTRLLRVLQQGEYTTVGGRT 267


45B7485_15265B7485_15350Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_15265215-1.333231murein transglycosylase B
B7485_15275115-1.267877PTS glucitol/sorbitol transporter subunit IIB
B7485_15285121-1.729288glucitol/sorbitol-specific phosphotransferase
B7485_15290121-2.010512sorbitol 6-phosphate dehydrogenase
B7485_15300128-5.516152glucitol operon repressor
B7485_15315-218-3.293276arabinose 5-phosphate isomerase GutQ
B7485_15320-112-2.000132nitric oxide reductase transcription regulator
B7485_15335212-0.821700anaerobic nitric oxide reductase
B7485_153401203.599412nitric oxide reductase FlRd-NAD+ reductase
B7485_153452203.438485carbamoyltransferase HypF
B7485_153502202.812634formate dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15575DHBDHDRGNASE828e-21 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 82.4 bits (203), Expect = 8e-21
Identities = 64/256 (25%), Positives = 116/256 (45%), Gaps = 5/256 (1%)

Query: 3 QVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQEINAEYGEGMAYGFGADA 62
++A + G Q +G + LA++G +A VD +K V + AE A F AD
Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE--ARHAEAFPADV 66

Query: 63 TSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQLGDFDRSLQVNLVGYFLCARE 122
++ ++ ++ G +D+LV AG+ + I +++ + VN G F +R
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 123 FSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFGGVGLTQSLALDLAEYGITVHSL 182
S+ M D G I+ + S V + Y+++K V T+ L L+LAEY I + +
Sbjct: 127 VSKYM-MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185

Query: 183 MLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDKVPLKRGCDYQDVLNMLLFYASPKA 242
G+ + + + IK +E + +PLK+ D+ + +LF S +A
Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGS-LETFKTG-IPLKKLAKPSDIADAVLFLVSGQA 243

Query: 243 SYCTGQSINVTGGQVM 258
+ T ++ V GG +
Sbjct: 244 GHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15585ARGREPRESSOR290.014 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 28.7 bits (64), Expect = 0.014
Identities = 20/105 (19%), Positives = 35/105 (33%), Gaps = 17/105 (16%)

Query: 1 MKPRQRQAAILEYLQKQGKCSVEEL-----AQYFDTTGTTIRKDLVILEHAGTVIRTYGG 55
M QR I E + + +EL ++ T T+ +D+ E + T G
Sbjct: 1 MNKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIK--ELHLVKVPTNNG 58

Query: 56 ---VVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIIL 97
L ++ P+ K + +A V I+L
Sbjct: 59 SYKYSLPADQRFNPLS-------KLKRSLMDAFVKIDSASHLIVL 96


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15595HTHFIS373e-127 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 373 bits (959), Expect = e-127
Identities = 125/388 (32%), Positives = 194/388 (50%), Gaps = 33/388 (8%)

Query: 149 IAALAAGALS----------NALLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLK 198
I A GA +I + ++ ++ ++G S M ++
Sbjct: 91 IKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIY 150

Query: 199 KEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFG 258
+ + + +DL ++I+GE+GTGKELVA+A+H+ R P V +N AA+P + ESELFG
Sbjct: 151 RVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFG 210

Query: 259 HVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLR 318
H KGAFTGA + +G+FE A+ GTLFLDEIG++ + Q +LLRVLQ G+ VG +R
Sbjct: 211 HEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIR 270

Query: 319 VDVRVLAATNRDLREEVLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRL 378
DVR++AATN+DL++ + G FR DL++RL+V PL +PPLR+R +D+ L +F +Q
Sbjct: 271 SDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAE- 329

Query: 379 RQGLSRVVLSAGARNLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVIL-----EAQH 433
++GL A L++ + +PGNVRELE+ + R L E+I E
Sbjct: 330 KEGLDVKRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPD 389

Query: 434 FAFPEVTLPPPEAAAVLVVKQNLR-----------------EATEAFQRETIRQALAQNH 476
+ + V++N+R + I AL
Sbjct: 390 SPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATR 449

Query: 477 HNWAACARMLETDVANLHRLAKRLGLKD 504
N A +L + L + + LG+
Sbjct: 450 GNQIKAADLLGLNRNTLRKKIRELGVSV 477


46B7485_15590B7485_15655Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_155901164.019247Zn-dependent exopeptidase M28
B7485_156001183.149072Hok/Gef family protein
B7485_156100194.417488phosphoadenosine phosphosulfate reductase
B7485_15615-1244.675879assimilatory sulfite reductase (NADPH)
B7485_15620-1235.036669assimilatory sulfite reductase (NADPH)
B7485_156250255.5229566-carboxytetrahydropterin synthase QueD
B7485_156300245.131904FAD-dependent oxidoreductase
B7485_156353204.728403ferredoxin family protein
B7485_156403204.166937MFS transporter
B7485_156453252.331895FAD-binding oxidoreductase
B7485_156502192.2412497-carboxy-7-deazaguanine synthase QueE
B7485_156552213.380792hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15840HOKGEFTOXIC543e-14 Hok/Gef cell toxic protein family signature.
		>HOKGEFTOXIC#Hok/Gef cell toxic protein family signature.

Length = 52

Score = 53.7 bits (129), Expect = 3e-14
Identities = 17/50 (34%), Positives = 28/50 (56%)

Query: 1 MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVLAYESKK 50
+ + ++++C T+L FT + SLCE+ R+ E A +AYES K
Sbjct: 3 LPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15855PF07675300.021 Cleaved Adhesin
		>PF07675#Cleaved Adhesin

Length = 1358

Score = 30.4 bits (68), Expect = 0.021
Identities = 20/92 (21%), Positives = 39/92 (42%), Gaps = 12/92 (13%)

Query: 206 ILGQTYLPRKFKTTVVIP---PQND--IDLHANDMNFVAIAENGKLVGFNLLVGGGLSIE 260
++ +P+ T +P PQN + A+ ++VAI+++G L G + G++
Sbjct: 240 VMPYRAMPKT--NTYTLPASLPQNQASYSIQASAGSYVAISKDGVLYGTGVANASGVATV 297

Query: 261 HGNK-----KTYARTASEFGYLPLEHTLAVAE 287
+ K Y + YLP+ + E
Sbjct: 298 NMTKQITENGNYDVVITRSNYLPVIKQIQAGE 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_15895TCRTETB340.001 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 33.7 bits (77), Expect = 0.001
Identities = 27/137 (19%), Positives = 55/137 (40%), Gaps = 11/137 (8%)

Query: 69 LGSLVLGWISDHIGRQKIFTFSFLLITLASFLQFFATTP-EHLIGLRILIGIGLGGDYSV 127
+G+ V G +SD +G +++ F ++ S + F + LI R + G G ++
Sbjct: 64 IGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPAL 123

Query: 128 GHTLLAEFSPRRHRGILLGAFSVVWT----VGYVLASIAGHHFISENPEAWRWLLASAAL 183
++A + P+ +RG G + VG + + H+ W +LL +
Sbjct: 124 VMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYI------HWSYLLLIPMI 177

Query: 184 PALLITLLRWGTPESPR 200
+ + L + R
Sbjct: 178 TIITVPFLMKLLKKEVR 194


47B7485_16265B7485_16350Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_16265326-3.504840oxidative stress defense protein
B7485_16270430-2.242733arginine exporter protein ArgO
B7485_162751034-3.633172small-conductance mechanosensitive channel
B7485_16280832-2.370546class II fructose-bisphosphate aldolase
B7485_16285830-3.082100phosphoglycerate kinase
B7485_16290221-1.348216erythrose-4-phosphate dehydrogenase
B7485_16295-118-0.918012erythrose 4-phosphate dehydrogenase
B7485_16300-116-0.546433ECF transporter S component
B7485_16310-1150.638737ABC transporter permease
B7485_16315-3141.072083energy-coupling factor transporter transmembrane
B7485_16320-2131.344217ABC transporter ATP-binding protein
B7485_163250211.031759ABC transporter ATP-binding protein
B7485_16335221-1.207377nucleoside/nucleotide kinase family protein
B7485_16340121-1.653811transcriptional regulator
B7485_16345114-1.687689fructose-bisphosphatase class II
B7485_16350216-0.914911L-sorbose 1-phosphate reductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16560SECYTRNLCASE280.040 Preprotein translocase SecY subunit signature.
		>SECYTRNLCASE#Preprotein translocase SecY subunit signature.

Length = 437

Score = 28.2 bits (63), Expect = 0.040
Identities = 34/154 (22%), Positives = 60/154 (38%), Gaps = 16/154 (10%)

Query: 34 ALAI--IIVGLIIARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGV 91
AL I I II ++++ + RL +K ++ RY +A ++ G V
Sbjct: 76 ALGIMPYITASIILQLLTVVIPRLEALKKEGQAGTAKITQYTRYLTVALAILQGTGL--V 133

Query: 92 QTASVIAVLGAAGLAVGLALQGSLSN-------LAAGVLLVMFRPFRAGEYVDLGGVAGT 144
TA + G + + S+ + AG +VM+ GE + G+ G
Sbjct: 134 ATARSAPLFGRCSVGGQIVPDQSIFTTITMVICMTAGTCVVMW----LGELITDRGI-GN 188

Query: 145 VLSVQIFSTTMRTADGKIIVIPNGKIIAGNIINF 178
+S+ +F + T + I +AG I F
Sbjct: 189 GMSILMFISIAATFPSALWAIKKQGTLAGGWIEF 222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16610PF05272280.025 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.025
Identities = 13/41 (31%), Positives = 16/41 (39%), Gaps = 12/41 (29%)

Query: 12 PGAATDCLCDISLQLKQGEWLALTGDNGAGKSTLLRVMAGL 52
PG D + L G G GKSTL+ + GL
Sbjct: 591 PGCKFDYS------------VVLEGTGGIGKSTLINTLVGL 619


48B7485_16520B7485_16590Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_165202130.049500L-asparaginase 2
B7485_165251110.294229hypothetical protein
B7485_16530013-0.914614DUF469 domain-containing protein
B7485_16535113-1.642612tRNA (guanosine(46)-N7)-methyltransferase TrmB
B7485_16540012-1.369696adenine glycosylase
B7485_16550026-0.509609A/G-specific adenine glycosylase
B7485_16560232-0.308313Fe(2+)-trafficking protein
B7485_165652320.724511lytic murein transglycosylase
B7485_16570-1211.555470nucleoside permease NupG
B7485_165753182.159824ornithine decarboxylase
B7485_165800193.382024hypothetical protein
B7485_16590-1153.010794transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16830TCRTETA290.028 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 29.4 bits (66), Expect = 0.028
Identities = 36/239 (15%), Positives = 76/239 (31%), Gaps = 18/239 (7%)

Query: 158 SHMQLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFS 217
H + AAL+ + L L + + L+ A F+ R
Sbjct: 159 PHAPFFAAAALNGLNFLTGCFLLPES---HKGERRPLRREALNPLASFRWARGMTVVAAL 215

Query: 218 MLLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLF-ILTIPFFLS 276
M + +Q+ F +D + + I ++ I +L + +
Sbjct: 216 MAVFFIMQLVGQVPAALWVIFGEDRFHWDATTI---GISLAAFGILHSLAQAMITGPVAA 272

Query: 277 RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKE 336
R G + +M+ ++A + L A+ + ++V + + + ++
Sbjct: 273 RLGERRALMLGMIADGTGYILLAF-----ATRGWMAFPIMVLLASGGIGMPALQAMLSRQ 327

Query: 337 VSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQ-TVWLIFAGYSVVL 394
V + QG +T+ L IV + IT W W+ A ++
Sbjct: 328 VDEERQGQLQGSLAALTS-----LTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLC 381


49B7485_16855B7485_16965Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_16855224-2.624063hypothetical protein
B7485_16860126-3.937051flavodoxin family protein
B7485_16865-117-3.813559quinol monooxygenase YgiN
B7485_16870-310-1.073852DNA topoisomerase IV subunit B
B7485_16875-311-0.459053esterase YqiA
B7485_16880-2121.283651phosphodiesterase
B7485_16885-1132.000249dehydrogenase
B7485_16890-1132.919307ADP-ribose diphosphatase
B7485_168950143.431368outer membrane channel protein TolC
B7485_169001143.269075hypothetical protein
B7485_169050133.059427hypothetical protein
B7485_169150160.922527glutathionylspermidine synthase family protein
B7485_16925012-0.605677dioxygenase
B7485_16930-112-0.153572zinc transporter ZupT
B7485_16940-1130.205032DUF4051 domain-containing protein
B7485_16945-2141.0735143,4-dihydroxy-2-butanone-4-phosphate synthase
B7485_16950-1161.640211hypothetical protein
B7485_169550182.131451glycogen synthase
B7485_169602232.304931hypothetical protein
B7485_169652252.453223bifunctional heptose 7-phosphate kinase/heptose
50B7485_17210B7485_17245Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_17210321-2.236639fimbrial protein
B7485_17215418-1.271580fimbrial protein
B7485_17220320-1.425751GntR family transcriptional regulator
B7485_17225221-0.930764DedA family protein
B7485_17230323-1.782198modulator protein MzrA
B7485_172401102.439829hypothetical protein
B7485_172452102.704708hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_17530PF00577681e-13 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 68.0 bits (166), Expect = 1e-13
Identities = 73/434 (16%), Positives = 142/434 (32%), Gaps = 32/434 (7%)

Query: 232 NSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGG 291
++V +N + + + G I+ S + + + E + T+ VP++
Sbjct: 308 TAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPL 367

Query: 292 LT-DGNAQWFLQAGKTTSQVS-DDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGN 349
L +G+ ++ + AG+ S + ++ +Q + L + +Y G AD AF G
Sbjct: 368 LQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGI 427

Query: 350 NWTADLGRVG--NLAISASVFRNDDGGKGDMQQANWS-NPGWPTLGF------YRTNSDG 400
++G +G ++ ++ + D + D Q + N G YR ++ G
Sbjct: 428 GK--NMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSG 485

Query: 401 ---DACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFE 457
A TT SR + + + + V F + + + + + + +
Sbjct: 486 YFNFADTTYSRMNGYNIETQDGV-IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLY 544

Query: 458 NNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFS--L 515
+ QT + + Q A D ++ ++ + D L L+ +
Sbjct: 545 LSGSHQTYWGTSNVDEQFQAGLNTAFED--INWTLSYSLTKNAWQKGRDQMLALNVNIPF 602

Query: 516 SDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYNDSFSHKEL--GVTVGGLNT 573
S DS + + + + T D+ + G GG
Sbjct: 603 SHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGN 662

Query: 574 DTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTD 633
+ G YGN S S + L S + GV LG D
Sbjct: 663 SGSTGYATLNYRGGYGNANIGYSHS---DDIKQLYY---GVSGGVLAHANGVTLGQPLND 716

Query: 634 DLLGAVLVDVKGFS 647
VLV G
Sbjct: 717 ---TVVLVKAPGAK 727



Score = 31.0 bits (70), Expect = 0.023
Identities = 40/222 (18%), Positives = 68/222 (30%), Gaps = 35/222 (15%)

Query: 246 SFYLNSGSQFIDTSSF------PPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQW 299
F + D S F PPG+Y V + + NN T V F
Sbjct: 52 RFLADDPQAVADLSRFENGQELPPGTYRVDIYL--NNGYMATRDVTFNTGDSEQG----- 104

Query: 300 FLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVG 359
+ T +Q++ +G+ L A A S D+G+
Sbjct: 105 -IVPCLTRAQLA-------SMGLNTASVSGMNLLADDACVPLTSMIH-DATAQLDVGQQR 155

Query: 360 -NLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYE 418
NL I + N +G + W L Y + + +R N+ Y
Sbjct: 156 LNLTIPQAFMSNRA--RGYIPPELWDPGINAGLLNYNFS----GNSVQNRIGGNSHYAY- 208

Query: 419 SISATVSLNFVGW----NMMLGYTCTQNNTDDSLRWDKQQSF 456
++ LN W N Y + +++ +W ++
Sbjct: 209 -LNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTW 249


51B7485_17640B7485_17680Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_17640015-3.048934transcription elongation factor GreA
B7485_17645119-6.276591serine-type D-Ala-D-Ala carboxypeptidase
B7485_17650122-8.162822GTPase ObgE
B7485_17655335-11.527434EamA family transporter
B7485_17660442-15.90349750S ribosomal protein L27
B7485_17665131-10.82015050S ribosomal protein L21
B7485_17670226-8.361595octaprenyl-diphosphate synthase
B7485_17675222-5.815652sugar fermentation stimulation protein B
B7485_17680013-3.517495UDP-N-acetylglucosamine
52B7485_17860B7485_17925Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_178601213.499869transcriptional regulator NanR
B7485_17870-1203.193164C4-dicarboxylate transporter
B7485_17875-1203.419269stringent starvation protein B
B7485_17880-1203.504099stringent starvation protein A
B7485_17885-1162.107767hypothetical protein
B7485_178901251.86454730S ribosomal protein S9
B7485_178952321.51176950S ribosomal protein L13
B7485_179004341.285177cell division protein ZapE
B7485_179053300.138921hypothetical protein
B7485_179105340.841642outer membrane-stress sensor serine
B7485_179156391.368519malate dehydrogenase
B7485_179205330.785741arginine repressor
B7485_179254300.761412hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18265V8PROTEASE538e-10 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 52.7 bits (126), Expect = 8e-10
Identities = 31/160 (19%), Positives = 59/160 (36%), Gaps = 26/160 (16%)

Query: 77 RTLGSGVIMDQRGYIITNKHVINDADQIIVALQ------------DGRVFEALLVGSDSL 124
+ SGV++ + ++TNKHV++ AL+ +G +
Sbjct: 101 TFIASGVVVG-KDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGE 159

Query: 125 TDLAVLKI-------NATGGLPTIPINARRVPHIGDVVLAIGNPYNLGQTITQGIISATG 177
DLA++K + + ++ + + G P + T + G
Sbjct: 160 GDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGD-KPVATMW--ESKG 216

Query: 178 RIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINT 217
+I + +Q D S GNSG + N E++GI+
Sbjct: 217 KI---TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHW 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18270DHBDHDRGNASE280.045 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 28.1 bits (62), Expect = 0.045
Identities = 37/167 (22%), Positives = 61/167 (36%), Gaps = 27/167 (16%)

Query: 3 VAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGED 62
+ GAA GIG+A+A L G+ ++ D P V S A + F +
Sbjct: 11 AFITGAAQGIGEAVARTL---ASQGAHIAAVDYNP-EKLEKVVSSLKAEARHAEAFPADV 66

Query: 63 ATPA------------LEGADVVLISAGVARK------PGMDRSDLFNVNAGIVKNLVQQ 104
A + D+++ AGV R + F+VN+ V N +
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 105 VAKNCPK----ACIGIITNPVNTT-VAIAAEVLKKAGVYDKNKLFGV 146
V+K + + + +NP ++AA KA K G+
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGL 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18275ARGREPRESSOR1694e-57 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 169 bits (430), Expect = 4e-57
Identities = 44/141 (31%), Positives = 71/141 (50%), Gaps = 5/141 (3%)

Query: 15 KALLKEEKFSSQGEIVAALQEQGFDNINQSKVSRMLTKFGAVRTRNAKMEMVYCLPAELG 74
+ ++ + +Q E+V L++ G+ N+ Q+ VSR + + V+ Y LPA+
Sbjct: 11 REIITANEIETQDELVDILKKDGY-NVTQATVSRDIKELHLVKVPTNNGSYKYSLPADQR 69

Query: 75 VPTTSSPLKNLV---LDIDYNDAVVVIHTSPGAAQLIARLLDSLGKAEGILGTIAGDDTI 131
S ++L+ + ID ++V+ T PG AQ I L+D+L E I+GTI GDDTI
Sbjct: 70 FNPLSKLKRSLMDAFVKIDSASHLIVLKTMPGNAQAIGALMDNLDWEE-IMGTICGDDTI 128

Query: 132 FTTPANGFTVKDLYEAILELF 152
K + + ILEL
Sbjct: 129 LIICRTHDDTKVVQKKILELL 149


53B7485_18550B7485_18770Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_18550326-0.297043hypothetical protein
B7485_185553210.090707aspartate aminotransferase family protein
B7485_18560524-0.422336glutamine amidotransferase
B7485_18570436-0.346364adenosine monophosphate-protein transferase
B7485_18575439-0.103652DUF2559 domain-containing protein
B7485_18580332-0.998893peptidylprolyl isomerase A
B7485_18585339-1.031291MFS transporter TsgA
B7485_18590238-1.070771nitrite reductase (NAD(P)H)
B7485_18595135-1.338511nitrite reductase small subunit
B7485_18600237-1.115586nitrite transporter NirC
B7485_18605339-0.766806siroheme synthase
B7485_18610447-0.175049lipoprotein
B7485_18615346-0.412995fructoselysine transporter
B7485_18620445-0.640794fructoselysine transporter
B7485_18625547-0.981048fructoselysine 6-phosphate deglycase
B7485_18630545-1.352895protein FrlC
B7485_18635643-2.143928fructoselysine 6-kinase
B7485_18640846-1.850554transcriptional regulator
B7485_18645647-1.871991aminotransferase
B7485_18650749-1.555278hypothetical protein
B7485_18655548-1.115722hypothetical protein
B7485_18660548-1.385684phosphotriesterase-related protein
B7485_18665551-0.430936phosphopentomutase
B7485_18675654-0.634203hypothetical protein
B7485_18680753-0.471425PRD domain-containing protein
B7485_186858530.164721hypothetical protein
B7485_186907510.157623tryptophan--tRNA ligase
B7485_18695637-0.852850phosphoglycolate phosphatase
B7485_18700330-2.195529ribulose-phosphate 3-epimerase
B7485_18705025-3.493454DNA adenine methylase
B7485_18710026-5.361303cell division protein DamX
B7485_18715030-3.3982233-dehydroquinate synthase
B7485_18720345-2.225720shikimate kinase
B7485_18725552-2.061829hypothetical protein
B7485_18730657-0.907590porin
B7485_18740655-0.485906DNA utilization protein HofP
B7485_18745445-0.598848DNA utilization protein HofO
B7485_18750225-1.416024DNA utilization protein HofN
B7485_18755323-2.003715DNA utilization protein HofM
B7485_18760320-2.230157carboxypeptidase/penicillin-binding protein 1A
B7485_18765323-1.266586ADP compounds hydrolase NudE
B7485_18770222-1.322885Intracellular growth attenuator protein igaA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18975TETREPRESSOR290.004 Tetracycline repressor protein signature.
		>TETREPRESSOR#Tetracycline repressor protein signature.

Length = 218

Score = 28.7 bits (64), Expect = 0.004
Identities = 12/58 (20%), Positives = 25/58 (43%), Gaps = 5/58 (8%)

Query: 1 METRLNLLCEAGVIDKDVCKGMMQVVN-----VLEKECHLPVRSEQGTMAMTHMASAL 53
+ET+L + E G +D + V + VLE++ H +++ ++ L
Sbjct: 113 VETQLRFMTENGFSLRDGLYAISAVSHFTLGAVLEQQEHTAALTDRPAAPDENLPPLL 170


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19005IGASERPTASE433e-06 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 42.7 bits (100), Expect = 3e-06
Identities = 39/199 (19%), Positives = 70/199 (35%), Gaps = 19/199 (9%)

Query: 143 DLAGNATDQANGVQPAPGTTSAENTQQDVSL-----------------PPISSTPTQGQT 185
DL ++ N T+ N Q DV PP +TP++
Sbjct: 979 DLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTE 1038

Query: 186 PVATDGQQRVEVQGDLNNALTQPQNQQQLNNVAVNSTLPTEPATVAPVRNGNASRDTAKT 245
VA + +Q + T+ Q + S + T ++G+ +++T T
Sbjct: 1039 TVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTT 1098

Query: 246 QTAERPSTTRPVRQQAVIEPKKPQATVKTEPKPVAQTPKRTEPAAPVASTKAPAATSTPA 305
+T E + + + + E + V ++ P + + +P A A P
Sbjct: 1099 ETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEP 1158

Query: 306 PKETATTAPVQTASPAQTT 324
+T TTA T PA+ T
Sbjct: 1159 QSQTNTTA--DTEQPAKET 1175



Score = 41.2 bits (96), Expect = 9e-06
Identities = 41/203 (20%), Positives = 68/203 (33%), Gaps = 10/203 (4%)

Query: 126 APSTTSSDQTASGEKSIDLAGNATDQANGVQPAPGTTSAENTQQDVSLPPISST-PTQGQ 184
P+ +D + + ++A D+A PAP T S + S T Q
Sbjct: 999 TPNNIQADVPSVPSNNEEIA--RVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQ 1056

Query: 185 TPVATDGQQRVEVQGDLNNALTQPQN----QQQLNNVAVNSTLPTEPATVAPVRNGNASR 240
T Q R + +N Q Q +T E ATV + A
Sbjct: 1057 DATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVE--KEEKAKV 1114

Query: 241 DTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEPKPVAQTPKRTEPAAPVASTKAPAA 300
+T KTQ + ++ +Q+ E +PQA E P + A T+ PA
Sbjct: 1115 ETEKTQEVPKVTSQVSPKQEQS-ETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAK 1173

Query: 301 TSTPAPKETATTAPVQTASPAQT 323
++ ++ T + +
Sbjct: 1174 ETSSNVEQPVTESTTVNTGNSVV 1196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19015CARBMTKINASE328e-04 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 32.1 bits (73), Expect = 8e-04
Identities = 27/91 (29%), Positives = 40/91 (43%), Gaps = 18/91 (19%)

Query: 32 FYDSDQEIEKRTGADVGWVFDLEGEEGFRD----------REEKVINELTEKQGIVLATG 81
FYD + KR + GW+ + G+R E + I +L E+ IV+A+G
Sbjct: 136 FYDEETA--KRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETIKKLVERGVIVIASG 193

Query: 82 GGSVKSRETRNRLSARGVVVYLETTIEKQLA 112
GG V + +GV E I+K LA
Sbjct: 194 GGGVPVILEDGEI--KGV----EAVIDKDLA 218


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19025TYPE3OMGPROT2871e-93 Type III secretion system outer membrane G protein ...
		>TYPE3OMGPROT#Type III secretion system outer membrane G protein

family signature.
Length = 607

Score = 287 bits (736), Expect = 1e-93
Identities = 80/301 (26%), Positives = 132/301 (43%), Gaps = 18/301 (5%)

Query: 117 LENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDNKTALSALEQWVAQMDL 176
L + +I D + +A SA+ + D N +++RD+ + ++ + +D
Sbjct: 219 LSDATIQQVTVDNQRIPQAAT-RASAQARVEADPSLNAIIVRDSPERMPMYQRLIHALDK 277

Query: 177 PVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNI 236
P ++E++ IV IN L ELGV W + + T G ++A+ G
Sbjct: 278 PSARIEVALSIVDINADQLTELGVDWRVGIRTGNNHQVVIKTTGDQSNIASN----GALG 333

Query: 237 GRINGRLLDL---ELSALEQKQQLDIIASPRLLASHLQPASIKQESEIPYQVSSGESGAT 293
++ R LD ++ LE + +++ P LL A I SE Y +G+ A
Sbjct: 334 SLVDARGLDYLLARVNLLENEGSAQVVSRPTLLTQENAQAVIDH-SETYYVKVTGKEVA- 391

Query: 294 SVEFKEAVLG--MEVTPTVLQKG---RIRLKLHISQNVPGQVLQQADGEVLAIDKQEIET 348
E K G + +TP VL +G I L LHI +G + I + ++T
Sbjct: 392 --ELKGITYGTMLRMTPRVLTQGDKSEISLNLHIEDGNQKPNSSGIEG-IPTISRTVVDT 448

Query: 349 QVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERRELVVFITPRL 408
V G++L +GGI+ + VPLLGDIP+ G LFR + R + I PR+
Sbjct: 449 VARVGHGQSLIIGGIYRDELSVALSKVPLLGDIPYIGALFRRKSELTRRTVRLFIIEPRI 508

Query: 409 V 409
+
Sbjct: 509 I 509



Score = 36.8 bits (85), Expect = 2e-04
Identities = 18/95 (18%), Positives = 33/95 (34%), Gaps = 4/95 (4%)

Query: 1 MKQWIAALLLMLIPGVQAA----KPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSG 56
K+ + LL+L A P + + +L +VVS ++
Sbjct: 9 FKRVLTGTLLLLSSYSWAQELDWLPIPYVYVAKGESLRDLLTDFGANYDATVVVSDKIND 68

Query: 57 TVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSV 91
VS + LQ + L+ +GN+L +
Sbjct: 69 KVSGQFEHDNPQDFLQHIASLYNLVWYYDGNVLYI 103


54B7485_19315B7485_19645Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_193150193.022507pili assembly chaperone protein SafB
B7485_19320-1213.129528LuxR family transcriptional regulator
B7485_19330-1233.068901ligase
B7485_193350253.672678hypothetical protein
B7485_19340-1253.377359transposase
B7485_193450273.250717aminoacetone oxidase family FAD-binding enzyme
B7485_19350-2263.512239anion permease
B7485_19360-1223.174622universal stress protein B
B7485_19365-1212.879647universal stress protein A
B7485_19375-1242.316817ribosomal RNA small subunit methyltransferase J
B7485_193800221.834360oligopeptidase A
B7485_193901202.29477923S rRNA (adenine(2030)-N(6))-methyltransferase
B7485_194001171.565627glutathione-disulfide reductase
B7485_194103131.607911Damage inducible protein
B7485_194152133.136888hypothetical protein
B7485_194200173.701230hypothetical protein
B7485_194250153.167900transcriptional regulator
B7485_19430-1163.333113arsenical efflux pump membrane protein ArsB
B7485_19435-1163.948954arsenate reductase
B7485_194401163.076151hypothetical protein
B7485_194452181.806054insertion element IS1 protein InsB
B7485_194501161.727802hypothetical protein
B7485_194551172.783706hypothetical protein
B7485_194601193.852125outer membrane protein slp
B7485_194651214.114490dctR protein
B7485_194700244.982686helix-turn-helix transcriptional regulator
B7485_194750244.862864acid stress chaperone HdeB
B7485_194801234.971164acid stress chaperone HdeA
B7485_194850193.484504protein HdeD
B7485_194900182.149081transcriptional regulator GadE
B7485_194950182.739033efflux RND transporter periplasmic adaptor
B7485_195000183.403571IS3 family transposase
B7485_19505014-0.603610Hok/Gef family protein
B7485_19510117-3.676979cold-shock protein
B7485_19515218-3.859614hypothetical protein
B7485_19520121-4.915045bifunctional glyoxylate/hydroxypyruvate
B7485_19525137-10.424244OmpA family lipoprotein
B7485_19530246-14.596977molybdopterin guanine dinucleotide-containing
B7485_19535444-14.711845N-acetyltransferase
B7485_19540240-11.667745DNA-3-methyladenine glycosylase I
B7485_19545337-10.271303autotransporter outer membrane beta-barrel
B7485_19550334-8.168832hypothetical protein
B7485_19555333-7.459227oxalate/formate antiport family MFS transporter
B7485_19560331-6.207405lipid A phosphoethanolamine transferase
B7485_19565330-5.553028*periplasmic dipeptide transporter
B7485_19570231-5.801588dipeptide ABC transporter permease DppB
B7485_19575026-4.523565dipeptide ABC transporter permease DppC
B7485_19580029-6.420739dipeptide transport ATP-binding protein DppD
B7485_19585-125-5.077360dipeptide ABC transporter ATP-binding protein
B7485_19590-123-4.457177transporter
B7485_19595-117-1.662181hypothetical protein
B7485_196050110.441574type I toxin-antitoxin system toxin Ldr family
B7485_196150120.094995hypothetical protein
B7485_19620-1141.308486small toxic polypeptide LdrD
B7485_19630-3181.958454type I toxin-antitoxin system toxin Ldr family
B7485_19635-3202.397428hypothetical protein
B7485_19640-2212.675967type I toxin-antitoxin system toxin Ldr family
B7485_19645-1243.489144cellulose biosynthesis protein BcsG
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19620ALARACEMASE290.031 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 29.0 bits (65), Expect = 0.031
Identities = 23/98 (23%), Positives = 38/98 (38%), Gaps = 18/98 (18%)

Query: 226 ENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFL--NEQRNAHPNQSLKNTL 283
E + RG GP +L + ++ + + + L T + N Q A N LK L
Sbjct: 63 EAITLRERGWKGP-ILMLEGFFHAQD---LEIYDQHRLTTCVHSNWQLKALQNARLKAPL 118

Query: 284 AVHL------------PKRLVERLQQLGQIPDVSLKQL 309
++L P R++ QQL + +V L
Sbjct: 119 DIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19625TYPE3IMSPROT320.006 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 32.0 bits (73), Expect = 0.006
Identities = 25/194 (12%), Positives = 57/194 (29%), Gaps = 40/194 (20%)

Query: 12 TGLLLLLALAFVLFYEAINGFHDTANAVATVIY------TRAMRSQLAVVMAAVFNFLGV 65
L++AL+ +L + F + + ++A+ + V+ F
Sbjct: 30 VSTALIVALSAMLMGLSDYYFEHFSKLMLIPAEQSYLPFSQALSYVVDNVLLEFFYLCFP 89

Query: 66 LLGGLSVAYAIVHML-------------------PTDLLLNMGSSHGLAMVFSMLLAAII 106
LL ++ H++ P + + S L +L ++
Sbjct: 90 LLTVAALMAIASHVVQYGFLISGEAIKPDIKKINPIEGAKRIFSIKSLVEFLKSILKVVL 149

Query: 107 WNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLIVSPIVG 166
++ W + ++ + T + T + + I L+V VG
Sbjct: 150 LSILIWIIIKG------NLVTLLQLP-TCGIECITPL--------LGQILRQLMVICTVG 194

Query: 167 LVFAGGLIFLLRRY 180
V + Y
Sbjct: 195 FVVISIADYAFEYY 208


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19670adhesinmafb240.035 Neisseria meningitidis: adhesin MafB signature.
		>adhesinmafb#Neisseria meningitidis: adhesin MafB signature.

Length = 467

Score = 24.3 bits (52), Expect = 0.035
Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 6/45 (13%)

Query: 21 VGNLTPARASVNGT----TRTSDQDFE--SVYAHCQSENASELTG 59
+GNL +A++NGT TR S E + + + +++ASE G
Sbjct: 78 MGNLLIQQANINGTIGYHTRFSGHGHEEHAPFDNHAADSASEEKG 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19765RTXTOXIND534e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 53.3 bits (128), Expect = 4e-10
Identities = 41/218 (18%), Positives = 70/218 (32%), Gaps = 33/218 (15%)

Query: 97 LQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSR-QDYDT-ARTQLNEAEANVT 154
+ + A L S + K Y Q + +L + N+
Sbjct: 257 QENKYVEAVNELRVYKSQLEQIE----SEILSAKEEYQLVTQLFKNEILDKLRQTTDNIG 312

Query: 155 VAKAAVEQATINLQYANVTSPITGVSGKSSV-TVGALVTANQADSLVTVQRLDPIYVDLT 213
+ + + Q + + +P++ + V T G +VT + +V V D + V
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAET-LMVIVPEDDTLEVTAL 371

Query: 214 QSVQDFLRMKEEVASGQIKQVQGSTPVQLNLE--NGKRY-SQTGTLK--FSDPTVDETTG 268
+D I + + +E RY G +K D D+ G
Sbjct: 372 VQNKD------------IGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLG 419

Query: 269 SVT--LRAI------FPNPNGDLLPGMYVTALVDEGSR 298
V + +I N N L GM VTA + G R
Sbjct: 420 LVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMR 457



Score = 33.3 bits (76), Expect = 0.001
Identities = 22/139 (15%), Positives = 48/139 (34%), Gaps = 24/139 (17%)

Query: 53 PGRTVPY-EVAEIRPQVGGIIIKRNFI-EGDKVNQGDSLYQIDPAPLQAELNSAKGSLAK 110
G+ EI+P I+ K + EG+ V +GD L ++ +A+ + SL +
Sbjct: 87 NGKLTHSGRSKEIKPIENSIV-KEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQ 145

Query: 111 ALSTASNAR-------------ITFNRQASL--------LKTNYVSRQDYDTARTQLNEA 149
A + + + + L+ + ++ + T + Q +
Sbjct: 146 ARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQK 205

Query: 150 EANVTVAKAAVEQATINLQ 168
E N+ +A +
Sbjct: 206 ELNLDKKRAERLTVLARIN 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19780HOKGEFTOXIC688e-20 Hok/Gef cell toxic protein family signature.
		>HOKGEFTOXIC#Hok/Gef cell toxic protein family signature.

Length = 52

Score = 67.5 bits (165), Expect = 8e-20
Identities = 18/50 (36%), Positives = 33/50 (66%)

Query: 1 MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFLAYKLKE 50
+P+ + ++++C TLL FT++ R SLCE+ + E+AAF+AY+ +
Sbjct: 3 LPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK 52


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19805OMPADOMAIN1111e-31 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 111 bits (280), Expect = 1e-31
Identities = 40/122 (32%), Positives = 61/122 (50%), Gaps = 11/122 (9%)

Query: 97 LNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEY--PKTAVNVIGYTDSTGGHDLNMRLS 154
+ ++V F+ + A LKP G L + L +V V+GYTD G N LS
Sbjct: 215 FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS 274

Query: 155 QQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGK---------AQNRRVEITL 205
++RA SV LI++G+ A +I +G+G +NP+ N+ K A +RRVEI +
Sbjct: 275 ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 206 SP 207

Sbjct: 335 KG 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19815SACTRNSFRASE355e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 34.9 bits (80), Expect = 5e-05
Identities = 16/52 (30%), Positives = 22/52 (42%), Gaps = 5/52 (9%)

Query: 76 VAPKAVRRGIGKALMQYV-----QQRYPHLMLEVYQKNQPAIDFYRAQGFHI 122
VA ++G+G AL+ + + LMLE N A FY F I
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19825ECOLNEIPORIN280.039 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 27.8 bits (62), Expect = 0.039
Identities = 19/90 (21%), Positives = 37/90 (41%), Gaps = 13/90 (14%)

Query: 119 SMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQISYNQQFGENIWKAQSGLS 178
S+ + D+ + H S + + + R G++ P ++SY F +
Sbjct: 228 SVAVQQQDAKLV-EENYSHNSQTEVAATLAYRFGNVTP--RVSYAHGFKGSF-------- 276

Query: 179 RMTATNQNGNWLDVTVGADMLLNQNIAAYA 208
ATN N ++ V VGA+ ++ +A
Sbjct: 277 --DATNYNNDYDQVVVGAEYDFSKRTSALV 304


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19835TCRTETA432e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 42.9 bits (101), Expect = 2e-06
Identities = 47/275 (17%), Positives = 94/275 (34%), Gaps = 32/275 (11%)

Query: 44 PVSQVAFSFGLL----SLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNL 99
+ V +G+L +L + V G L +RFG + V + S + + + A + L
Sbjct: 37 HSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFL 96

Query: 100 MMLWLS---AGVLVGLADGAGYLL----TLSNCVKWFPERKGLISAFAIGSYGLGSLGFK 152
+L++ AG+ AG + + F + LG
Sbjct: 97 WVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGG---- 152

Query: 153 FIDTQLLETVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMR 212
L+ F A+ + + G L+ ++ K E + R
Sbjct: 153 -----LMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWAR 207

Query: 213 --KPQYWMLAVMFLTACMSG----LYVIGVAKDIAQSLAHLDVVSAANAVTVISIAN-LS 265
++AV F+ + L+VI + H D + ++ I + L+
Sbjct: 208 GMTVVAALMAVFFIMQLVGQVPAALWVI-----FGEDRFHWDATTIGISLAAFGILHSLA 262

Query: 266 GRLVLGILSDKIARIRVITIGQVISLVGMAALLFA 300
++ G ++ ++ R + +G + G L FA
Sbjct: 263 QAMITGPVAARLGERRALMLGMIADGTGYILLAFA 297



Score = 36.0 bits (83), Expect = 2e-04
Identities = 37/155 (23%), Positives = 64/155 (41%), Gaps = 9/155 (5%)

Query: 241 AQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFA 300
AH ++ A A+ + A + G L SD+ R V+ + + V A + A
Sbjct: 39 NDVTAHYGILLALYALMQFACAPVLGAL-----SDRFGRRPVLLVSLAGAAVDYAIMATA 93

Query: 301 PLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSIFGSIIA 360
P V + I VA G T V + +++ + A+++G + FG G + G ++
Sbjct: 94 PFLWVLYIGRI--VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG 151

Query: 361 SLFGGF--YVTFYVIFALLILSLALSTTIRQPEQK 393
L GGF + F+ AL L+ + K
Sbjct: 152 GLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHK 186


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19920FLGMRINGFLIF320.008 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 31.9 bits (72), Expect = 0.008
Identities = 10/44 (22%), Positives = 19/44 (43%), Gaps = 5/44 (11%)

Query: 94 QGSQVAGFSTDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIR 137
G ++ + ID + W + VL+VAW+ + +R
Sbjct: 442 TGGELPFWQQQSFIDQLLAAGRW-----LLVLVVAWILWRKAVR 480


55B7485_19690B7485_19805Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_19690-127-6.456612peptidase M16
B7485_19695229-7.069425sugar kinase
B7485_19700331-9.223924hypothetical protein
B7485_19705231-10.423347cyclic-guanylate-specific phosphodiesterase
B7485_19710334-11.206231AsmA family protein
B7485_19720227-9.545982MFS transporter
B7485_19730124-10.459457inner membrane protein YhjD
B7485_19735226-9.171605LysR family transcriptional regulator
B7485_19745-219-4.294039helix-turn-helix transcriptional regulator
B7485_19755225-5.347777IS4 family transposase
B7485_19765223-3.763545trehalase
B7485_19770222-4.777462cytochrome-c peroxidase
B7485_19775020-4.567276glutamate decarboxylase alpha
B7485_19780-116-0.722608transcriptional regulator
B7485_197850111.412919transcriptional regulator
B7485_197901111.537002hypothetical protein
B7485_197951111.720829hypothetical protein
B7485_198001131.692859glycine--tRNA ligase subunit beta
B7485_198052141.430085glycine--tRNA ligase subunit alpha
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20015SALSPVBPROT320.003 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 31.6 bits (71), Expect = 0.003
Identities = 43/165 (26%), Positives = 65/165 (39%), Gaps = 32/165 (19%)

Query: 93 DFFVEHGLLASVNIDGPTLIALRQQPKILRQIERLPWLRFELV----EHIRLPKDSTFAS 148
DF++ H +++ G T A P+ + WL E V EHI ++
Sbjct: 157 DFWLLHDSNGILHLLGKTAAARLSDPQAASHTAQ--WLVEESVTPAGEHI------YYSY 208

Query: 149 MCEFGPLWLDDFGTGMANFSA---LSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLM 205
+ E G + + SA LS+V+Y A +L++ +P + LF L+
Sbjct: 209 LAENGDNVDLNGNEAGRDRSAMRYLSKVQYGNATPAADLYLWTSATPAVQWLF----TLV 264

Query: 206 NRYC-RGVIVEGVETPEEWRDVQNSPAFAAQGWFLSRPAPMETLN 249
Y RGV D Q PAF AQ +L+R P N
Sbjct: 265 FDYGERGV------------DPQVPPAFTAQNSWLARQDPFSLYN 297


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20025TCRTETB347e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.5 bits (79), Expect = 7e-04
Identities = 85/405 (20%), Positives = 137/405 (33%), Gaps = 72/405 (17%)

Query: 79 IGSAVFGHFGDRVGRKATLVASLLTMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLG 138
IG+AV+G D++G K LL GI G + G+ F+ LL +ARF QG G
Sbjct: 64 IGTAVYGKLSDQLGIK-----RLLLFGIIINCFGSVIGFVGHSFFS--LLIMARFIQGAG 116

Query: 139 LGGEWGGAALLATENAPPRKR----ALYGSFPQLGAPIGFFFANGTFLLLSW-------- 186
++ P R L GS +G +G + W
Sbjct: 117 AAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM 176

Query: 187 -----LLTDEQFMSWGWRV--PF-IFSAVLVIIG-------------LYVRVSLHESPVF 225
+ + + R+ F I +L+ +G ++ VS+ +F
Sbjct: 177 ITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIF 236

Query: 226 EKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFSTAAAPVGLGL 285
K + + G + VL I+ T F M Y M + +G
Sbjct: 237 VKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIG- 295

Query: 286 PRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIIL-FALFAFNPLLGSGN 344
+ +++ M+VI FG + G+L D G + I T + + F +F S
Sbjct: 296 --SVIIFPGTMSVIIFG---YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWF 350

Query: 345 PILVFAFLLLGLSLMGL---TFGPMGALLPELFPTEVRYTGASFS-YNVSSILGASVAPY 400
++ F+L GLS T L E GA S N +S L
Sbjct: 351 MTIIIVFVLGGLSFTKTVISTIVSSS-----LKQQEA---GAGMSLLNFTSFLSEGTGIA 402

Query: 401 IAAWL-------------QANYGLGAVGLYLAAMAGLTLIALLLT 432
I L + + L +G+ +I+ L+T
Sbjct: 403 IVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVT 447



Score = 29.1 bits (65), Expect = 0.041
Identities = 13/73 (17%), Positives = 29/73 (39%), Gaps = 2/73 (2%)

Query: 283 LGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALFAFNPLLGS 342
P W+ ++ F + V G L+D G ++ ++ + ++ F + S
Sbjct: 44 FNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGF--VGHS 101

Query: 343 GNPILVFAFLLLG 355
+L+ A + G
Sbjct: 102 FFSLLIMARFIQG 114


56B7485_19875B7485_19950Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_19875125-3.702223protein bax
B7485_198804410.316353alpha-amylase
B7485_198853441.627847valine--pyruvate transaminase
B7485_198904421.566954electron transporter
B7485_199001200.271186IclR family transcriptional regulator
B7485_19910115-1.1561663-dehydro-L-gulonate 2-dehydrogenase
B7485_19920013-0.464017YhcH/YjgK/YiaL family protein
B7485_19925-1130.698537phage tail protein
B7485_19930-2141.433114L-dehydroascorbate transporter large permease
B7485_19940-3192.765232TRAP transporter substrate-binding protein DctP
B7485_19945-3213.262280carbohydrate kinase
B7485_19950-2203.8026783-keto-L-gulonate-6-phosphate decarboxylase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20165FLGFLGJ391e-05 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 38.5 bits (89), Expect = 1e-05
Identities = 31/105 (29%), Positives = 46/105 (43%), Gaps = 17/105 (16%)

Query: 134 TRKIPWNTLLERVDIIPTSMVATMAAAESGWGTSKLARNN----NNLFGMKC---MKGRC 186
+ L + +P ++ AA ESGWG ++ R N NLFG+K KG
Sbjct: 154 AQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPV 213

Query: 187 T---------NAPGKVKG-YSQFSSVKESVSAYVTNLNTHPAYSS 221
T KVK + +SS E++S YV L +P Y++
Sbjct: 214 TEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAA 258


57B7485_20330B7485_20495Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_20330-115-3.391339guanylate kinase
B7485_20335235-2.577092DNA-directed RNA polymerase subunit omega
B7485_20340338-2.891107bifunctional (p)ppGpp synthetase II/
B7485_20345328-1.541463tRNA (guanosine(18)-2'-O)-methyltransferase
B7485_20355121-0.347492DNA helicase RecG
B7485_20365-1142.569073sodium/glutamate symport carrier protein
B7485_20370-1143.250456xanthine permease XanP
B7485_203750142.850624AsmA family protein
B7485_203800152.730351alpha-xylosidase
B7485_203900151.359203*integrase
B7485_20395-1231.428709hypothetical protein
B7485_204001231.562631hypothetical protein
B7485_204102171.724180hypothetical protein
B7485_20420-1161.718339hypothetical protein
B7485_20430-212-1.697632IS3 family transposase
B7485_20435-113-2.016321hypothetical protein
B7485_20440-214-1.387116hypothetical protein
B7485_20445-112-1.618351shiE
B7485_20450-116-4.870435MFS transporter
B7485_20455126-8.196620aerobactin synthase IucA
B7485_20460227-9.285457N-acetyltransferase
B7485_20465337-11.957066IucA/IucC family siderophore biosynthesis
B7485_20470446-15.832971lysine 6-monooxygenase
B7485_20475446-16.612974TonB-dependent siderophore receptor
B7485_20480240-14.084383hypothetical protein
B7485_20485334-12.234223serine protease
B7485_20490027-8.897899IS3 family transposase
B7485_20495119-5.051809transposase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20625SECA421e-05 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 41.8 bits (98), Expect = 1e-05
Identities = 38/129 (29%), Positives = 56/129 (43%), Gaps = 18/129 (13%)

Query: 233 NLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIEHDM-ALDVPMM 291
LS L+ F A+ L + L+N + A A R ++ M DV ++
Sbjct: 37 KLSDEELKGKTAEFRAR-LEKGEVLENLIPEAF------AVVREASKRVFGMRHFDVQLL 89

Query: 292 ---RLVQGDV-----GSGKTLVAALAA-LRAIAHGKQVALMAPTELLAEQHANNFRNWFA 342
L + + G GKTL A L A L A+ GK V ++ + LA++ A N R F
Sbjct: 90 GGMVLNERCIAEMRTGEGKTLTATLPAYLNALT-GKGVHVVTVNDYLAQRDAENNRPLFE 148

Query: 343 PLGIEVGWL 351
LG+ VG
Sbjct: 149 FLGLTVGIN 157


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20740TCRTETA485e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 47.5 bits (113), Expect = 5e-08
Identities = 81/375 (21%), Positives = 135/375 (36%), Gaps = 41/375 (10%)

Query: 20 FSAGLLGIGQNGLLVVLPVLVIQTNLSLSV---WAALLMLGSMLFLPSSPWWGKQISRTG 76
+ L +G ++ VLP L+ S V + LL L +++ +P G R G
Sbjct: 12 STVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFG 71

Query: 77 SKPVVLWALGGYGISFTLLGLGSVLMATSAITTAVGLGILIIARIAYGLTVSAMVPACQV 136
+PV+L +L G + + ++ L +L I RI G+T + A
Sbjct: 72 RRPVLLVSLAGAAVDYAIMATAPFLW------------VLYIGRIVAGITGATGAVAGAY 119

Query: 137 WALQRAGEGNRMAALATISSGLSCGRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLL 196
A R +S+ G + GP+ M P AP A L L
Sbjct: 120 IA-DITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGC 178

Query: 197 RL------PGTPPQPTPECKSVSLKRDCLPYLLCAILLAAAVSMMQLGLSPAL------T 244
L P ++ R + A L+A M +G PA
Sbjct: 179 FLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGE 238

Query: 245 RQFATDTTAISQQVAWLLGLSAVAALIAQFGVLRPQRLTPVALLLSAGVLMSGGLAIMLS 304
+F D T I +A L ++A + G + + AL+L +G + + +
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMI-TGPVAARLGERRALMLGMIADGTGYILLAFA 297

Query: 305 EQLWLFYPGCAVLSFGAALATPAYQLLLNDKLADGAGAGWLATSHTLGYGLCALLVPLVS 364
+ W+ +P +L+ G + PA Q +L+ + D G L L L S
Sbjct: 298 TRGWMAFPIMVLLASG-GIGMPALQAMLS-RQVDEERQGQLQ----------GSLAALTS 345

Query: 365 KTGVAIALIMAALFA 379
T + L+ A++A
Sbjct: 346 LTSIVGPLLFTAIYA 360


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20745PF04183339e-111 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 339 bits (872), Expect = e-111
Identities = 104/480 (21%), Positives = 178/480 (37%), Gaps = 46/480 (9%)

Query: 58 ELLIPLDEQKSLHFRVAYFSPTQHHRF-----AFPARLVTASGSYPVDFTTLSRLIIDKL 112
E + + Q + + P RF + + A D L++ ++ +L
Sbjct: 24 EQVFHAESQGDDRYCIN--LPGAQWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQL 81

Query: 113 RHQLFLPVPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHP 172
+ L + Q + + + Q + AR +A LN + Q LL+GH
Sbjct: 82 KQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASDLINLNA-DRLQCLLSGHPKFV 140

Query: 173 APKSHEPFNRREAERYLPDMAPHFPLRWFSVDKTQIAGES-LHLNLQQRLTRFAAENAPQ 231
K + + ERY P+ A F L W +V + + +++ Q LT A PQ
Sbjct: 141 FNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEMDIHQLLT---AAMDPQ 197

Query: 232 LLNELS--------DNQWLF-PLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTT 282
S D+ WL P+HPWQ + + + A+G + LGE G WL
Sbjct: 198 EFARFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADF-AEGRMVSLGEFGDQWLAQQ 256

Query: 283 SSRSLYCATSRD--MIKFSLSVRLTNSIRTLSVKEVKRGMRLARLAQ----TDGWQMLQ- 335
S R+L A+ R IK L++ T+ R + + + G +R Q TD +
Sbjct: 257 SLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSG 316

Query: 336 ---VRFPTFRVMQEDGWAGLLDLNGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPD 392
+ P + +G+A L + REN ++ VL++ +
Sbjct: 317 AVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDE 376

Query: 393 GGDSLLVSAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILV 452
L + + DR G+ A W+ + V+ PL+ YG+ L+AH QNI +
Sbjct: 377 NNQPLAGAYI----DRSGLD----AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITL 428

Query: 453 QMLGDLPVGFIYRDCQGSAFMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFA 512
M +P + +D QG M + + E + L++
Sbjct: 429 AMKEGVPQRVLLKDFQGD--MRLVKEEFPEMDSLPQE----VRDVTSRLSADYLIHDLQT 482


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20755PF041838160.0 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 816 bits (2109), Expect = 0.0
Identities = 565/580 (97%), Positives = 571/580 (98%)

Query: 1 MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGAQWRFIAERGIWGWLWI 60
MNHKDWD VNRRLVAKMLSE+EYEQVFHAESQGDD YCINLPGAQWRFIAERGIWGWLWI
Sbjct: 1 MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGAQWRFIAERGIWGWLWI 60

Query: 61 DAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASD 120
DAQTLRC DEPVLAQTLLMQLK VLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASD
Sbjct: 61 DAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASD 120

Query: 121 LINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYTNTFRLHWLAVKREHMIWRC 180
LINL+ADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY NTFRLHWLAVKREHMIWRC
Sbjct: 121 LINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRC 180

Query: 181 DNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEG 240
DN++DI QLLTAAMDPQEF RFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEG
Sbjct: 181 DNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEG 240

Query: 241 RMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASR 300
RMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASR
Sbjct: 241 RMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASR 300

Query: 301 WLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLK 360
WLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLK
Sbjct: 301 WLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLK 360

Query: 361 PDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALI 420
PDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALI
Sbjct: 361 PDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALI 420

Query: 421 AHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEAFPEMDSLPQEVRDVTSRLSADYLIHDL 480
AHGQNITLAMKEGVPQRVLLKDFQGDMRLVKE FPEMDSLPQEVRDVTSRLSADYLIHDL
Sbjct: 421 AHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMDSLPQEVRDVTSRLSADYLIHDL 480

Query: 481 QTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMNKHPQMAERFALFSLFRPQIIR 540
QTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYM KHPQM+ERFALFSLFRPQIIR
Sbjct: 481 QTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMKKHPQMSERFALFSLFRPQIIR 540

Query: 541 VVLNPVKLTWPDLDGGSRMLPNYLENLQNPLWLVTQEYES 580
VVLNPVKLTWPDLDGGSRMLPNYLE+LQNPLWLVTQEYES
Sbjct: 541 VVLNPVKLTWPDLDGGSRMLPNYLEDLQNPLWLVTQEYES 580


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20775IGASERPTASE834e-20 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 82.8 bits (204), Expect = 4e-20
Identities = 48/214 (22%), Positives = 74/214 (34%), Gaps = 52/214 (24%)

Query: 31 NRKLVATMLSLAVAGTVNA---ANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGD 87
N+K ++L VA + A + +V + + D A+NKG F GAT+V + KN
Sbjct: 3 NKKFKLNFIALTVAYALTPYTEAALVRDDVDYQIFRDFAENKGKFSVGATNVLVKDKNNK 62

Query: 88 KF--SFHN-LSIPDFSGAAAS-GAATAIGGSYSVTVAH-----------------NKKNP 126
+ N + + DFS AT I Y V V H N N
Sbjct: 63 DLGTALPNGIPMIDFSVVDVDKRIATLINPQYVVGVKHVSNGVSELHFGNLNGNMNNGNA 122

Query: 127 QAAETQVYAQSSYKVVDRRNSN-------------------DFEIQRLNKFVVETVGATP 167
+A ++ Y V++ D+ + RL+KFV E
Sbjct: 123 KAHRDVSSEENRYFSVEKNEYPTKLNGKTVTTEDQTQKRREDYYMPRLDKFVTEVAPIEA 182

Query: 168 AETNPTTYSDALERYGIVTSDGSKKIIGFRAGSG 201
+ + +D +K R GSG
Sbjct: 183 STAS---------SDAGTYNDQNKYPAFVRLGSG 207


58B7485_20560B7485_20855Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_205602151.206426ilvB operon leader peptide IvbL
B7485_20565215-0.546808hypothetical protein
B7485_20570-115-0.596104hypothetical protein
B7485_20580-114-0.872979LexA family transcriptional regulator
B7485_20585016-1.214020type I toxin-antitoxin system toxin TisB
B7485_20590-2120.049992multidrug transporter EmrD
B7485_20595-2121.283284transcriptional regulator
B7485_20600-2112.406811hypothetical protein
B7485_20605-1142.686855hypothetical protein
B7485_20610-1163.222517hypothetical protein
B7485_20615-1143.146082solute:sodium symporter family transporter
B7485_20620-1142.684471AraC family transcriptional regulator
B7485_20625-2131.900536PTS alpha-glucoside transporter subunit IICB
B7485_20630-190.037707transcriptional regulator
B7485_20635-37-0.859159transporter
B7485_20640-39-1.703685heat-shock protein IbpB
B7485_20645-212-2.970669heat-shock protein IbpA
B7485_20650-120-3.178588YceK/YidQ family lipoprotein
B7485_20660127-4.133854hypothetical protein
B7485_20665-126-1.553974protein CbrA
B7485_20670031-0.817958MFS transporter
B7485_20675030-0.881852galactonate dehydratase
B7485_206801280.219924galactonate dehydratase
B7485_20685230-1.0000492-dehydro-3-deoxy-6-phosphogalactonate aldolase
B7485_20690228-0.4471252-oxo-3-deoxygalactonate kinase
B7485_20695129-2.970096transcriptional regulator
B7485_20700129-3.897266hypothetical protein
B7485_20705128-2.640103sugar phosphatase YidA
B7485_20710233-7.711194hypothetical protein
B7485_20715528-3.575854DNA gyrase subunit B
B7485_20720422-1.501800DNA replication and repair protein RecF
B7485_20725420-0.043851DNA polymerase III subunit beta
B7485_207304181.182886chromosomal replication initiation protein DnaA
B7485_207354190.114577hypothetical protein
B7485_207403181.40197450S ribosomal protein L34
B7485_207453170.592959ribonuclease P protein component
B7485_20750318-0.900447membrane protein insertion efficiency factor
B7485_20760424-5.454261membrane protein insertase YidC
B7485_20765325-4.963129tRNA uridine-5-carboxymethylaminomethyl(34)
B7485_20770233-7.473932tryptophanase leader peptide
B7485_20775027-5.869384tryptophanase
B7485_20780024-5.147500low affinity tryptophan permease
B7485_20790017-1.973134multidrug resistance protein MdtL
B7485_20795117-0.366563DNA-binding transcriptional regulator
B7485_208000140.348450transposase
B7485_208050140.839679transcriptional regulator
B7485_20815-1152.246486hypothetical protein
B7485_20820-1163.182468chromate reductase
B7485_208250173.563936NCS2 family permease
B7485_208301184.2531106-phosphogluconate phosphatase
B7485_208351173.710704protein CbrB
B7485_208401182.494823transposase
B7485_208451161.434911IS66 family transposase
B7485_208552201.295926hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20880TCRTETB606e-12 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 59.9 bits (145), Expect = 6e-12
Identities = 41/184 (22%), Positives = 81/184 (44%), Gaps = 1/184 (0%)

Query: 5 RNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGAVQSVMGAYLLTYGVSQLFYG 64
R+ +L+ L +L + + + ++ D+A D N + V A++LT+ + YG
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 65 PISDRVGRRPVILVGMSIFMLATLVA-VTTSSLTVLIAASAMQGMGTGVGGVMARTLPRD 123
+SD++G + ++L G+ I +++ V S ++LI A +QG G + +
Sbjct: 71 KLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVAR 130

Query: 124 LYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWNWRACYLFLLVLCAGVTFSMARWM 183
+ A L+ + + + P IGG++ +W L ++ V F M
Sbjct: 131 YIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLK 190

Query: 184 PETR 187
E R
Sbjct: 191 KEVR 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20965TCRTETA423e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 42.1 bits (99), Expect = 3e-06
Identities = 63/383 (16%), Positives = 112/383 (29%), Gaps = 34/383 (8%)

Query: 51 AEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIAIFGWSVVTLFQGFATGLMSLIGL 110
A G + + +A + C G DR G R +++ G +V A L L
Sbjct: 43 AHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG 102

Query: 111 RAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQFVGLAFLTPLLIWIQEMLSWH 170
R + GI A + ERA GF ++ G+ P+L + S H
Sbjct: 103 RIVAGITGAT-GAVAGAYIADITDGDERARHFGFMSACFGFGMV-AGPVLGGLMGGFSPH 160

Query: 171 WVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDYIRDGGGLVDGDAPVKKEARQPLT 230
F + + L + + P+++EA PL
Sbjct: 161 APFFAAAALNGLNFLTGCFLLPESHKGER-------------------RPLRREALNPLA 201

Query: 231 AKDWKLVFHRKLIGVYLGQFAVASALWFFLTWFPNYLTQEKGITALKAGFMTTVPFLAAF 290
+ W + + F + + + A G
Sbjct: 202 SFRWARGM-TVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAA---FGI 257

Query: 291 VGVLLSGWVADLLVRKGFSLGFARKTPIICGLLISTC--IMGANYTNDPMMIMCLMALAF 348
+ L + + + + ++ G++ I+ A T M ++ LA
Sbjct: 258 LHSLAQAMITGPVAAR-----LGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLAS 312

Query: 349 FGNGFASITWSLVSSLAPMRLIGLTGGVFNFAGGLGGITVPLVVGYL-AQGYGFAPALVY 407
G G ++ +++S G G L I PL+ + A +
Sbjct: 313 GGIGMPALQ-AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAW 371

Query: 408 ISAVALIGALSYILLVGDVKRVG 430
I+ AL L G G
Sbjct: 372 IAGAALYLLCLPALRRGLWSGAG 394


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_2105060KDINNERMP8730.0 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 873 bits (2258), Expect = 0.0
Identities = 547/548 (99%), Positives = 547/548 (99%)

Query: 1 MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKL 60
MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKL
Sbjct: 1 MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKL 60

Query: 61 ISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTGRDGP 120
ISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTGRDGP
Sbjct: 61 ISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTGRDGP 120

Query: 121 DNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNTFTKTFVLKRGDYAVNVNYNV 180
DNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNTFTKTFVLKRGDYAVNVNYNV
Sbjct: 121 DNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNTFTKTFVLKRGDYAVNVNYNV 180

Query: 181 QNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTFRGAAYSTPDEKYEKYKFDTIAD 240
QNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTFRGAAYSTPDEKYEKYKFDTIAD
Sbjct: 181 QNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTFRGAAYSTPDEKYEKYKFDTIAD 240

Query: 241 NENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYTANLGNGIVAIGYKSQPVLVQPGQT 300
NENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYTANLGNGI AIGYKSQPVLVQPGQT
Sbjct: 241 NENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYTANLGNGIAAIGYKSQPVLVQPGQT 300

Query: 301 GAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIII 360
GAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIII
Sbjct: 301 GAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIII 360

Query: 361 ITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPL 420
ITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPL
Sbjct: 361 ITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPL 420

Query: 421 GGCFPLLIQMPIFLALYYMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQK 480
GGCFPLLIQMPIFLALYYMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQK
Sbjct: 421 GGCFPLLIQMPIFLALYYMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQK 480

Query: 481 MSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGL 540
MSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGL
Sbjct: 481 MSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGL 540

Query: 541 HSREKKKS 548
HSREKKKS
Sbjct: 541 HSREKKKS 548


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_21080TCRTETA543e-10 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 54.1 bits (130), Expect = 3e-10
Identities = 60/247 (24%), Positives = 94/247 (38%), Gaps = 7/247 (2%)

Query: 2 SRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAML----F 57
+R LI + V L GI + + LP + DL S + + + LA A
Sbjct: 4 NRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSN-DVTAHYGILLALYALMQFACAPV 62

Query: 58 AGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILR 117
G ++DR GR+PV + A + + A + GR + G+ G VA A +
Sbjct: 63 LGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIA 121

Query: 118 DTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWAMAMMGIAVLMLSLFI 177
D D RA+ ++ V PVLG L M F + F+A A + + F+
Sbjct: 122 DITDGDERARHFGFMSACFGFGMVAGPVLGGL-MGGFSPHAPFFAAAALNGLNFLTGCFL 180

Query: 178 LKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFER 237
L E+ + N + VV ++V I+ V P L I G +R
Sbjct: 181 LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDR 240

Query: 238 GEYATIM 244
+
Sbjct: 241 FHWDATT 247


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_21135RTXTOXIND418e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.4 bits (97), Expect = 8e-06
Identities = 9/74 (12%), Positives = 33/74 (44%), Gaps = 1/74 (1%)

Query: 16 LRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLFGQSSEKKRHKLENQIRQAEKRLS 75
+ +Q+++ + ++ Y+ ++E++++++ + + K L+ ++RQ +
Sbjct: 254 VLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILD-KLRQTTDNIG 312

Query: 76 ELENRLNTARNLLE 89
L L +
Sbjct: 313 LLTLELAKNEERQQ 326


59B7485_21110B7485_21275Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_21110218-0.735800acetolactate synthase 2 catalytic subunit
B7485_21115222-2.045778acetolactate synthase isozyme 2 small subunit
B7485_21120322-1.903760branched chain amino acid aminotransferase
B7485_21125222-1.273942dihydroxy-acid dehydratase
B7485_21130118-2.694847PLP-dependent threonine dehydratase
B7485_21135117-3.219294transcriptional regulator IlvY
B7485_21140116-3.302371ketol-acid reductoisomerase
B7485_21145116-3.660178peptidyl-prolyl cis-trans isomerase
B7485_21150118-4.206542DNA helicase Rep
B7485_21155015-4.172394guanosine-5'-triphosphate,3'-diphosphate
B7485_21160-113-2.800739addiction module toxin RelE
B7485_21165-215-1.214259ATP-dependent RNA helicase RhlB
B7485_21170-219-0.656931thiol reductase thioredoxin
B7485_21175-2280.997687rho operon leader peptide
B7485_21180-122-0.742591transcription termination factor Rho
B7485_21190015-1.104564hypothetical protein
B7485_21195118-3.465130undecaprenyl-phosphate
B7485_21200225-3.386152LPS biosynthesis protein
B7485_21205133-4.428244UDP-N-acetylglucosamine 2-epimerase
B7485_21210030-3.057964UDP-N-acetyl-D-mannosamine dehydrogenase
B7485_21215126-2.448798dTDP-glucose 4,6-dehydratase
B7485_21220015-1.335047glucose-1-phosphate thymidylyltransferase 2
B7485_21225-1120.429613TDP-fucosamine acetyltransferase
B7485_21230-1140.349181dTDP-4-amino-4,6-dideoxygalactose transaminase
B7485_212352231.718630lipid III flippase WzxE
B7485_212401242.194671TDP-N-acetylfucosamine:lipid II
B7485_212453372.046105O-antigen assembly polymerase
B7485_212503351.847318lipopolysaccharide
B7485_212554401.901524amino acid permease
B7485_212604421.795501****anaerobic sulfatase maturase AslB
B7485_212653340.696033IS3 family transposase
B7485_212704350.433887heme biosynthesis protein HemY
B7485_21275220-0.886807uroporphyrinogen-III C-methyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_21550NUCEPIMERASE1825e-57 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 182 bits (464), Expect = 5e-57
Identities = 84/353 (23%), Positives = 145/353 (41%), Gaps = 44/353 (12%)

Query: 3 KILITGGAGFIGSALVRYIINETSDAVVVVDKLT--YAGNL-MSLAPVAQSERFAFEKVD 59
K L+TG AGFIG + + ++ E VV +D L Y +L + + F F K+D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLL-EAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 60 ICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEAARAYWNTL 119
+ DR + +F + V V S++ P A+ ++N+ G +LE R
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN---- 116

Query: 120 TEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPYSASKASSDHLVRAWLRTYG 179
+ S+ VYG L+ F T+ + P S Y+A+K +++ + + YG
Sbjct: 117 -----KIQHLLYASSSSVYG-LNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170

Query: 180 LPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYGNGQQIRDWLYVEDHARALYCV 239
LP YGP+ P+ + L GK + VY G+ RD+ Y++D A A+ +
Sbjct: 171 LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRL 230

Query: 240 ------------------ATTGKVGETYNIGGHNERKNLDVVETICELLEELASNKPHGV 281
A + YNIG + + +D ++ + + L
Sbjct: 231 QDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDAL------GIEAK 284

Query: 282 AHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN 334
+ L +PG L + D + +G+ P+ T + G++ V WY
Sbjct: 285 KNMLPL------QPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_21560SACTRNSFRASE373e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 36.8 bits (85), Expect = 3e-05
Identities = 26/126 (20%), Positives = 45/126 (35%), Gaps = 10/126 (7%)

Query: 104 FAQSRFRAPWYAPDASGRFYAQWIEN---AVRGTFDHQCLILRAA-SGDIRGYVSLRELN 159
+ + RF P++ ++E A + I R + GY + ++
Sbjct: 37 YTEERFSKPYFKQYEDDDMDVSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIEDIA 96

Query: 160 -ATDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST 218
A D R +G G L+ A+ WA L + TQ N +A Y + + +
Sbjct: 97 VAKDYR-----KKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAV 151

Query: 219 AYWLYR 224
LY
Sbjct: 152 DTMLYS 157


60B7485_21385B7485_21500Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_21385-120-3.114266DNA helicase RecQ
B7485_21390-120-3.167501threonine export protein RhtC
B7485_21425-217-1.165231homoserine/homoserine lactone efflux protein
B7485_21430-116-0.854335lysophospholipase
B7485_21435-1140.044653pyridoxal phosphate phosphatase YigL
B7485_21440-1182.126233EamA family transporter
B7485_214450214.055380transcriptional regulator
B7485_214500214.3026455-methyltetrahydropteroyltriglutamate--
B7485_214550264.114857carboxymethylenebutenolidase
B7485_214600274.171277uridine phosphorylase
B7485_214650233.747242hypothetical protein
B7485_214700203.140868DNA recombination protein RmuC
B7485_214750181.934622ubiquinone/menaquinone biosynthesis
B7485_21480-1161.175605ubiquinone biosynthesis protein UbiJ
B7485_21485-1130.923263ubiquinone biosynthesis regulatory protein
B7485_21490-1120.940191twin-arginine translocase subunit TatA
B7485_21495017-0.113671twin-arginine translocase subunit TatB
B7485_21500222-1.648534twin-arginine translocase subunit TatC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_21835TATBPROTEIN2014e-69 Bacterial sec-independent translocation TatB protein...
		>TATBPROTEIN#Bacterial sec-independent translocation TatB protein

signature.
Length = 171

Score = 201 bits (512), Expect = 4e-69
Identities = 171/171 (100%), Positives = 171/171 (100%)

Query: 1 MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLATTVQNELTQELKLQEFQ 60
MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLATTVQNELTQELKLQEFQ
Sbjct: 1 MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLATTVQNELTQELKLQEFQ 60

Query: 61 DSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPEKASDEAHTIHNPVVKDNE 120
DSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPEKASDEAHTIHNPVVKDNE
Sbjct: 61 DSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPEKASDEAHTIHNPVVKDNE 120

Query: 121 AAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKTAAPSPSSSDKP 171
AAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKTAAPSPSSSDKP
Sbjct: 121 AAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKTAAPSPSSSDKP 171


61B7485_21680B7485_21725Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_21680-1204.057767hypothetical protein
B7485_21685-2183.242686MFS transporter
B7485_21690-2163.063029porin
B7485_21695-3152.680058permease
B7485_21700-2152.457738alpha-glucosidase
B7485_21705-1131.006969aldose epimerase
B7485_21710214-3.646281AGE family epimerase/isomerase
B7485_21715014-2.810502sulfofructosephosphate aldolase
B7485_21720-113-4.239073ribokinase
B7485_21725-113-3.710557DeoR/GlpR transcriptional regulator
62B7485_21855B7485_22025Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_21855-1183.0352182-keto-3-deoxygluconate permease
B7485_21860-1182.834425IS3 family transposase
B7485_21865-1192.782771MOSC domain-containing protein
B7485_21870-1181.674362two-component sensor histidine kinase
B7485_21875-2140.671284DNA-binding response regulator
B7485_21880-115-0.173422periplasmic protein CpxP
B7485_21885015-1.479536cation-efflux pump FieF
B7485_21890-115-2.830249ATP-dependent 6-phosphofructokinase
B7485_21925-218-5.907229sulfate-binding protein
B7485_21930-119-7.155204CDP-diacylglycerol pyrophosphatase
B7485_21935-214-4.325734triose-phosphate isomerase
B7485_21940-214-4.084389triosephosphate isomerase
B7485_21945-214-4.044156hypothetical protein
B7485_21950-115-1.961248hypothetical protein
B7485_21955116-0.539986universal stress protein D
B7485_219601160.206400ferredoxin--NADP(+) reductase
B7485_219700162.332361fructose 1,6-bisphosphatase
B7485_219750152.252280glycerol kinase
B7485_219800162.101040glycerol transporter
B7485_21985-1152.063691aquaporin
B7485_219902231.904567cell division protein ZapB
B7485_219953240.575601ribonuclease E activity regulator RraA
B7485_22000423-1.4710401,4-dihydroxy-2-naphthoate
B7485_22005220-2.403441HslU--HslV peptidase ATPase subunit
B7485_22010114-2.833259HslU--HslV peptidase proteolytic subunit
B7485_22015114-3.198650cell division protein FtsN
B7485_22020-120-5.818917transcriptional regulator
B7485_22025-121-4.650485primosomal protein N'
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22250ACRIFLAVINRP290.023 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.4 bits (66), Expect = 0.023
Identities = 38/172 (22%), Positives = 67/172 (38%), Gaps = 17/172 (9%)

Query: 160 TAGIASFEPHVFVGAVLPFLVGFA-LGNLDPELREFFSKAVQTLIPF-FAFALGNTID-L 216
I +F +L FLV + L N+ L + V L F A G +I+ L
Sbjct: 334 QLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTL 393

Query: 217 TVIAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAV--ATPVLIA 274
T+ +G+L+ A+++V + ++ + A + S A+ VL A
Sbjct: 394 TMFGMVLAIGLLVDDAIVVVENVERVMMED--KLPPKEATEKSMSQIQGALVGIAMVLSA 451

Query: 275 EMVPA----------FKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKAR 316
+P ++ + S +A +V+V IL P L + + V A
Sbjct: 452 VFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAE 503


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22265PF06580290.027 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.4 bits (66), Expect = 0.027
Identities = 28/186 (15%), Positives = 63/186 (33%), Gaps = 33/186 (17%)

Query: 277 IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEV-LDNAAFEAEQM--GKSLT 333
I + + M+ L + R + + + L E+ + ++ + + L
Sbjct: 186 ILEDPTKAREMLTSLSELMRYSLRYSNARQV----SLADELTVVDSYLQLASIQFEDRLQ 241

Query: 334 VNF--PPGPWPLYGNPNALESALENIVRNAL--RYSHTKIEVGFAVDKDGITITVDDDGP 389
P + P +++ +EN +++ + KI + D +T+ V++ G
Sbjct: 242 FENQINPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGS 301

Query: 390 GVSPEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGW---VKAEDSPLGGLR 446
+E TG GL V +Q G +K + G +
Sbjct: 302 LALKNTKE------------------STGTGLQNVRERLQMLYGTEAQIKLSEKQ-GKVN 342

Query: 447 LVIWLP 452
++ +P
Sbjct: 343 AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22270HTHFIS929e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 92.2 bits (229), Expect = 9e-24
Identities = 35/117 (29%), Positives = 62/117 (52%), Gaps = 2/117 (1%)

Query: 3 KILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLL-DDSIDLLLLDVMMPKKNGID 61
IL+ DDD + ++L + L G++V + + + DL++ DV+MP +N D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 TLKALRQTH-QTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRR 117
L +++ PV++++A+ + + + E GA DYLPKPF+ EL+ I L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22370HTHFIS300.017 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.2 bits (68), Expect = 0.017
Identities = 11/36 (30%), Positives = 18/36 (50%), Gaps = 3/36 (8%)

Query: 49 TPKNILMIGPTGVGKTEIAR---RLAKLANAPFIKV 81
T +++ G +G GK +AR K N PF+ +
Sbjct: 159 TDLTLMITGESGTGKELVARALHDYGKRRNGPFVAI 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22380IGASERPTASE422e-06 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 42.0 bits (98), Expect = 2e-06
Identities = 32/155 (20%), Positives = 64/155 (41%), Gaps = 5/155 (3%)

Query: 114 LTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQQLAEQQRLAQQSR 173
+ +QAD+ P+ E+ ++ P + +AE + Q+S+
Sbjct: 992 VDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSK--QESK 1049

Query: 174 TTEQSWQQQT-RTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTTAQSKPQQAAPVARA 232
T E++ Q T T+Q V + + + A+TQ + T ++ ++ A V +
Sbjct: 1050 TVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKE 1109

Query: 233 ADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQ 267
A T + ++ + Q + EQ+ETV+ Q
Sbjct: 1110 EKAKVETEKTQEVPKVTSQVSPKQ--EQSETVQPQ 1142


63B7485_22100B7485_22125Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_22100-226-4.947598formate C-acetyltransferase
B7485_22105-229-5.834959[formate-C-acetyltransferase]-activating enzyme
B7485_22110-136-8.569570PTS fructose-like transporter subunit EIIB
B7485_22115-137-7.766865AraC family transcriptional regulator
B7485_22120029-6.494048phosphoethanolamine transferase CptA
B7485_22125021-3.292132phosphoenolpyruvate carboxylase
64B7485_22375B7485_22420Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_223753133.183700DNA-binding response regulator
B7485_223802133.242205phosphoribosylamine--glycine ligase
B7485_223851142.520939bifunctional
B7485_223903153.297990*acetyltransferase
B7485_224000183.488937homoserine O-succinyltransferase
B7485_22405-2203.939278malate synthase
B7485_22410-2203.877320malate synthase
B7485_22415-2183.718877isocitrate lyase
B7485_22420-2153.715878IclR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22780HTHFIS5250.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 525 bits (1355), Expect = 0.0
Identities = 183/468 (39%), Positives = 253/468 (54%), Gaps = 35/468 (7%)

Query: 8 ILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIAT 67
ILV DDD + T+L L GY+V + ++ + DLV+ DV M + +
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 68 LKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQDYLIKPLDFDNLQATLEKALAHTHSI 127
L IK P +PVL+M+A ++ TA++A + GA DYL KP D L + +ALA
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR 125

Query: 128 DAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVARAIHASSA 187
++ + +VG+S AMQ + +A + ++ T++I G+SGTGKELVARA+H
Sbjct: 126 PSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGK 185

Query: 188 RSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDEIGDISP 247
R P V +N AA+ L+ESELFGHEKGAFTGA R GRF +A+GGTLFLDEIGD+
Sbjct: 186 RRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPM 245

Query: 248 MMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAI 307
Q RLLR +Q+ E VG I DVR++AAT++DL +N G FR+DLYYRLNVV +
Sbjct: 246 DAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVVPL 305

Query: 308 EVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVER 367
+P LR R EDIP L HF+Q+ + VK F +A++L+ + WPGN+RELEN V R
Sbjct: 306 RLPPLRDRAEDIPDLVRHFVQQAEKEGLD-VKRFDQEALELMKAHPWPGNVRELENLVRR 364

Query: 368 AVVLLTGEYISERELPLAIASTPIPLGQSQDIQP-------------------------- 401
L + I+ + + S +
Sbjct: 365 LTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALP 424

Query: 402 --------LVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR 441
L E+E +ILAAL T GN+ +AA LG+ R TL K+
Sbjct: 425 PSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22820SACTRNSFRASE310.001 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 30.7 bits (69), Expect = 0.001
Identities = 15/54 (27%), Positives = 20/54 (37%), Gaps = 5/54 (9%)

Query: 78 IDPDVCGCGVGRMLVEHALSMAPE-----LTTNVNEQNEQAVGFYKKVGFKVTG 126
+ D GVG L+ A+ A E L + N A FY K F +
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22840BINARYTOXINB320.004 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 32.3 bits (73), Expect = 0.004
Identities = 14/58 (24%), Positives = 23/58 (39%)

Query: 289 ETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIASFQQQLSDMGYK 346
ET+ PD+ L A P L Y + N D +T + + QL+++
Sbjct: 544 ETTKPDMTLKEALKIAFGFNEPNGNLQYQGKDITEFDFNFDQQTSQNIKNQLAELNAT 601


65B7485_22575B7485_22890Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_22575-123-4.483563DNA-binding response regulator
B7485_22600022-3.741147two-component sensor histidine kinase
B7485_22605123-2.594941PmrR protein
B7485_22610429-2.312194proline/betaine transporter
B7485_22615344-0.159289hypothetical protein
B7485_226204490.241067clamp-binding protein CrfC
B7485_226455490.440483hypothetical protein
B7485_226504400.039778protein PhnA
B7485_22655750-1.182765VOC family protein
B7485_226607500.019736phosphonates import ATP-binding protein PhnC
B7485_226658520.881370phosphonate ABC transporter substrate-binding
B7485_226707441.196796membrane channel protein of phosphonate
B7485_226756421.720183hypothetical protein
B7485_226806421.803681hypothetical protein
B7485_226856382.262029AraC family transcriptional regulator
B7485_226902283.341106redox-sensitive transcriptional activator SoxR
B7485_22695-2154.466173hypothetical protein
B7485_22700-1164.142531guanine/hypoxanthine permease GhxP
B7485_227100153.446204Na+/H+ antiporter
B7485_227151173.002882pentapeptide repeat protein
B7485_227201183.106539cation acetate symporter
B7485_227251171.744925hypothetical protein
B7485_227300141.863262acetyl-coenzyme A synthetase
B7485_227351152.030711hypothetical protein
B7485_227401171.120553ammonia-forming cytochrome c nitrite reductase
B7485_227451171.088361cytochrome c nitrite reductase pentaheme
B7485_227500130.548176protein NrfC
B7485_227550152.493776cytochrome c nitrite reductase subunit NrfD
B7485_227601173.730081heme lyase subunit NrfE
B7485_227650194.048762heme lyase NrfEFG subunit NrfF
B7485_227750194.034491heme lysase NrfEFG subunit NrfG
B7485_227850182.447548hypothetical protein
B7485_227900161.143646glutamate/aspartate:proton symporter GltP
B7485_22795-1160.525532hypothetical protein
B7485_22825-115-3.434376hypothetical protein
B7485_22835-113-0.691663multidrug resistance outer membrane protein
B7485_22845-111-0.330828multidrug transporter subunit MdtO
B7485_22850-110-0.433662multidrug transporter subunit MdtN
B7485_22860-1193.062709ssDNA-binding protein
B7485_22865-2161.783991excinuclease ABC subunit A
B7485_228750160.163962hypothetical protein
B7485_22880016-0.688938thiamin phosphate synthase
B7485_22885216-1.049298acid phosphatase AphA
B7485_22890317-1.853382aspartate/tyrosine/aromatic aminotransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22995HTHFIS912e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 90.7 bits (225), Expect = 2e-23
Identities = 40/121 (33%), Positives = 59/121 (48%)

Query: 2 KILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGHYSLVVLDLGLPDEDGLH 61
IL+ +DD + L A GY + A + + G LVV D+ +PDE+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 FLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKPFALEELHARIRALLRRHNN 121
L RI++ + LPVL+++A++T I + GA DYL KPF L EL I L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 122 Q 122
+
Sbjct: 125 R 125


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23000PF06580371e-04 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 36.8 bits (85), Expect = 1e-04
Identities = 40/182 (21%), Positives = 80/182 (43%), Gaps = 34/182 (18%)

Query: 181 ARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLEDV-ILPSYDELSTML--DQRQQTLL 237
+ +M+ S+S+L++ S N + V L +++ ++ SY +L+++ D+ Q
Sbjct: 191 TKAREMLTSLSELMR-----YSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQ 245

Query: 238 LPESAADITVQGDATLLRMLLRNLVENAHRY----SPQGSNIMIKLQEDGGAV-MAVEDE 292
+ + D+ V ML++ LVEN ++ PQG I++K +D G V + VE+
Sbjct: 246 INPAIMDVQV------PPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENT 299

Query: 293 GPGIDESKCGELSKAFVRMDSRYGGIGLGLSIV-SRITQLHHGQFFLQNRQETSGTRAWV 351
G + + G GL V R+ L+ + ++ ++ A V
Sbjct: 300 GSLA--------------LKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMV 345

Query: 352 RL 353
+
Sbjct: 346 LI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23010TCRTETA432e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 42.9 bits (101), Expect = 2e-06
Identities = 57/290 (19%), Positives = 105/290 (36%), Gaps = 55/290 (18%)

Query: 85 FFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGE 144
G L D++GR+ +L +++ ++ + P +W +L I ++ G + G
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPF-----LW---VLYIGRIVAGIT-GAT 112

Query: 145 YTGASIFVAEYSPDRKR----GFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGW 200
A ++A+ + +R GFM + FG +AG VLG G++ S
Sbjct: 113 GAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG-GLMGGFSP------------ 159

Query: 201 RIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRS 260
PFF A L + L K E+ P SF+ W
Sbjct: 160 HAPFFAAAALNGLNFLTGCFLLPESH------KGERRPLRREALNPLASFR------WAR 207

Query: 261 LLTCIGLVIATNVTYYML----LTYMPSYLSHNLHYS-EDHGVLIIIAIMIGMLFVQPVM 315
+T + ++A ++ + H+ G+ + ++ L +
Sbjct: 208 GMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMIT 267

Query: 316 GLLSDRFGRRPFVLLG----SVALFVLA--------IPAFILINSNVIGL 353
G ++ R G R ++LG +LA P +L+ S IG+
Sbjct: 268 GPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGM 317



Score = 41.0 bits (96), Expect = 8e-06
Identities = 39/164 (23%), Positives = 73/164 (44%), Gaps = 16/164 (9%)

Query: 286 LSHNLHYSEDHGVLI-IIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFI 344
L H+ + +G+L+ + A+M PV+G LSDRFGRRP +L+ L A+ I
Sbjct: 35 LVHSNDVTAHYGILLALYALM--QFACAPVLGALSDRFGRRPVLLVS---LAGAAVDYAI 89

Query: 345 LINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIR---YSALAAAFNISVLVAG 401
+ + + +++ G ++A I V + + + R + ++A F +VAG
Sbjct: 90 MATAPFLWVLYIG-RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFG-MVAG 147

Query: 402 LTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITG-VTMKETANR 444
P L + S + P + + + +TG + E+
Sbjct: 148 --PVLGGLMGGFSPH--APFFAAAALNGLNFLTGCFLLPESHKG 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23040PF05272290.019 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.019
Identities = 12/22 (54%), Positives = 13/22 (59%)

Query: 32 MVALLGPSGSGKSTLLRHLSGL 53
V L G G GKSTL+ L GL
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23115RTXTOXIND270.020 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 26.7 bits (59), Expect = 0.020
Identities = 5/33 (15%), Positives = 13/33 (39%), Gaps = 1/33 (3%)

Query: 17 ELVEKR-QRFATILSIIMLAVYIGFILLIAFAP 48
EL+E R +++ ++ + +L
Sbjct: 47 ELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQ 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23140VACJLIPOPROT300.006 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 29.9 bits (67), Expect = 0.006
Identities = 6/21 (28%), Positives = 11/21 (52%)

Query: 179 FGNLDDPNSEISQLLRQKPTY 199
GNL++P ++ L+ P
Sbjct: 75 TGNLEEPAVMVNYFLQGDPYQ 95


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23200RTXTOXIND725e-16 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 71.8 bits (176), Expect = 5e-16
Identities = 51/363 (14%), Positives = 112/363 (30%), Gaps = 78/363 (21%)

Query: 8 APRSKFPALLVVALALVALVFVIW-RVDS-APSTNDAYASADTIDVVPEVSGRIVELAVT 65
+ R + A ++ ++A + + +V+ A + S + ++ P + + E+ V
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 66 DNQAVKQGDLLFRIDPRPYEANLAKAEAS-----LAALDKQIMLTQRSVDAQQFGADSVN 120
+ ++V++GD+L ++ EA+ K ++S L QI+ ++
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 121 ATVEKARAAAKQATDTL------------------------------RRTEPLLKEGFVS 150
+ +L R V
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 151 AEDVDRARTAQRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLH----- 205
+D + +AVL Q AV+ + +Q +E++I K
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 206 -------------------------------LEMTTVRAPFDGRIISLKT-SVGQFASAM 233
+ + +RAP ++ LK + G +
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 234 RPIFTLIDTRHWYVI-ANFRETDLKNIRSGTPATIRLMSDSGKTF---EGKVDSIGYGVL 289
+ ++ + A + D+ I G A I++ + + GKV +I +
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINLDAI 413

Query: 290 PDD 292
D
Sbjct: 414 EDQ 416


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23215PERTACTIN270.048 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 27.0 bits (59), Expect = 0.048
Identities = 15/50 (30%), Positives = 19/50 (38%), Gaps = 4/50 (8%)

Query: 119 GGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSG----GAQSRPQQSAPAAP 164
G APAGG + GG GG + + G + QS AP
Sbjct: 261 GDAPAGGAVPGGAVPGGAVPGGFGPLLDGWYGVDVSDSTVDLAQSIVEAP 310


66B7485_23120B7485_23390Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_23120-2213.149761hypothetical protein
B7485_23125-1163.147586hypothetical protein
B7485_23130-2163.586980isoaspartyl dipeptidase
B7485_23135-1184.041454LysR family transcriptional regulator
B7485_23140-1193.895594anti-adapter protein IraD
B7485_23145-1183.071150hypothetical protein
B7485_231500141.651748hypothetical protein
B7485_23155-3180.838449GntR family transcriptional regulator
B7485_23160-3180.566988hypothetical protein
B7485_23165-2161.390023fructuronate reductase
B7485_23175-1142.346808mannonate dehydratase
B7485_23185-1192.548170hypothetical protein
B7485_23195-2143.385239gluconate permease
B7485_23200-1163.302728fimbrial protein
B7485_232050152.732500fimbrial protein
B7485_232100132.256929type 1 fimbrial protein
B7485_23215-1151.396072fimbrial biogenesis outer membrane usher
B7485_23220014-0.321131molecular chaperone FimC
B7485_23225022-3.743962type 1 fimbrial protein
B7485_23230018-1.787099type-1 fimbrial protein subunit A
B7485_23235014-0.894699type 1 fimbriae regulatory protein FimE
B7485_23240111-0.318568integrase
B7485_232451120.502583hypothetical protein
B7485_232500131.516492N-acetylneuraminic acid outer membrane channel
B7485_23255012-0.437826N-acetylneuraminate epimerase
B7485_23260113-2.682909sialate O-acetylesterase
B7485_23265019-3.583034transposase
B7485_23270121-4.142443DeoR family transcriptional regulator
B7485_23275021-4.588370acetolactate synthase
B7485_23280120-3.167185IS3 family transposase
B7485_232901141.267111IS3 family transposase
B7485_233000151.857240hypothetical protein
B7485_233050142.108613transposase
B7485_23310-1161.323667IS3 family transposase
B7485_23315116-4.352293integrase
B7485_23320-114-3.693633*aldehyde reductase Ahr
B7485_23325-117-5.992625hypothetical protein
B7485_23330015-5.007256LPS export ABC transporter permease LptG
B7485_23335016-5.490803lipopolysaccharide ABC transporter permease
B7485_23340014-4.457750cytosol aminopeptidase
B7485_23345-1200.937471DNA polymerase III subunit chi
B7485_23355-1190.313965valine--tRNA ligase
B7485_23360019-0.445325hypothetical protein
B7485_23365019-0.531276N-acetyltransferase
B7485_23370-1150.431627regulator of ribonuclease activity B
B7485_233800100.575248ornithine carbamoyltransferase
B7485_23390-1163.080109DNA-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23480UREASE354e-04 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 35.1 bits (81), Expect = 4e-04
Identities = 21/85 (24%), Positives = 37/85 (43%), Gaps = 20/85 (23%)

Query: 26 CDVLVANGKIIAVASNIPSDIVPNCT--------VVDLSGQILCPGFIDQHVHLIGGGGE 77
D+ + +G+I A+ D+ P T V+ G+I+ G +D H+H I
Sbjct: 86 ADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEGKIVTAGGMDSHIHFI----- 140

Query: 78 AGPTTRTPEVALSRLTEAGVTSVVG 102
+ E AL +G+T ++G
Sbjct: 141 ---CPQQIEEALM----SGLTCMLG 158


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23535PF06580310.008 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 31.4 bits (71), Expect = 0.008
Identities = 10/49 (20%), Positives = 25/49 (51%)

Query: 230 LVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFV 278
+ +I ++ I +W V +T W ++ FI + P+A + + ++ +
Sbjct: 73 MGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPLALSII 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23540SURFACELAYER280.047 Lactobacillus surface layer protein signature.
		>SURFACELAYER#Lactobacillus surface layer protein signature.

Length = 439

Score = 28.1 bits (62), Expect = 0.047
Identities = 19/79 (24%), Positives = 32/79 (40%), Gaps = 1/79 (1%)

Query: 211 SQNLGYYLSGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVS 270
S+N G ++ +A+ N FT PA V V L ++G ++ + + +
Sbjct: 133 SENAGKEITIGSAN-PNVTFTEKTGDQPASTVKVTLDQDGVAKLSSVQIKNVYAIDTTYN 191

Query: 271 LGLTANYARTGGQVTAGNV 289
+ TG VT G V
Sbjct: 192 SNVNFYDVTTGATVTTGAV 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23545VACCYTOTOXIN334e-04 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 33.5 bits (76), Expect = 4e-04
Identities = 30/158 (18%), Positives = 49/158 (31%), Gaps = 9/158 (5%)

Query: 3 WCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAG 62
W R + A LA + +TI + VT VN + + + + G
Sbjct: 258 WMGRLQYVGAYLAPSYSTINTSKVTGEVNFNHLTVGDHNAAQAGIIASNKTH------IG 311

Query: 63 AASAWHDVALELTNCPVG--TSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLN 120
W L + P G + S + Q ++QN + N+
Sbjct: 312 TLDLWQSAGLNIIAPPEGGYKDKPNDKPSNTTQNNAKNDKQESSQNNSNTQVINPPNSAQ 371

Query: 121 TGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQA 158
+ QV D + V +N A GTI+
Sbjct: 372 KTEIQPTQVIDGPFAGGKNTVVNINRINTNA-DGTIRV 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23555PF0057710860.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 1086 bits (2810), Expect = 0.0
Identities = 866/878 (98%), Positives = 871/878 (99%)

Query: 1 MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSSADLYFNLRFLADDPQA 60
MSYLNLRLYQRNTQCLHIRKHRLAGFFVRL VACAFAAQAPLSSA+LYFN RFLADDPQA
Sbjct: 1 MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQA 60

Query: 61 VADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLN 120
VADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLN
Sbjct: 61 VADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLN 120

Query: 121 TASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTIPQAFMSNRARGYIPPELWDP 180
TASV+GMNLLADDACVPLT+M+ DATA LDVGQQRLNLTIPQAFMSNRARGYIPPELWDP
Sbjct: 121 TASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDP 180

Query: 181 GINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSK 240
GINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSK
Sbjct: 181 GINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSK 240

Query: 241 NKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV 300
NKWQHI TW+ERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
Sbjct: 241 NKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV 300

Query: 301 IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTV 360
IHGIARGTAQVTIKQNGY IYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTV
Sbjct: 301 IHGIARGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTV 360

Query: 361 PYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY 420
PYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY
Sbjct: 361 PYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY 420

Query: 421 RAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYR 480
RAFNFGIGKNM ALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYR
Sbjct: 421 RAFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYR 480

Query: 481 YSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRT 540
YSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRT
Sbjct: 481 YSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRT 540

Query: 541 STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600
STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI
Sbjct: 541 STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600

Query: 601 PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD 660
PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD
Sbjct: 601 PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD 660

Query: 661 GNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVL 720
GNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVL
Sbjct: 661 GNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVL 720

Query: 721 VKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVP 780
VKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVP
Sbjct: 721 VKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVP 780

Query: 781 TRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA 840
TRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA
Sbjct: 781 TRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA 840

Query: 841 GKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR 878
GKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR
Sbjct: 841 GKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR 878


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23635HTHFIS270.013 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 26.7 bits (59), Expect = 0.013
Identities = 7/45 (15%), Positives = 16/45 (35%), Gaps = 1/45 (2%)

Query: 4 KRYPEEFKTEAVKQVVDR-GYSVASVATRLDITTHSLYAWIKKYG 47
R E + + + + A L + ++L I++ G
Sbjct: 430 DRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELG 474


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23725SACTRNSFRASE326e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 32.2 bits (73), Expect = 6e-04
Identities = 15/48 (31%), Positives = 18/48 (37%)

Query: 97 PAIRGKGLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGF 144
R KG+ L A+E A+E F LET A Y F
Sbjct: 99 KDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146


67B7485_23440B7485_23645Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_234402270.047030magnesium-translocating P-type ATPase
B7485_23445127-0.034399trehalose operon repressor
B7485_23450-124-0.198809PTS trehalose transporter subunit IIBC
B7485_23455-2171.243429glucohydrolase
B7485_234600170.719911anaerobic ribonucleoside triphosphate reductase
B7485_234700161.039654anaerobic ribonucleotide reductase-activating
B7485_23475-1151.108465soluble cytochrome b562
B7485_23480-116-1.571188metalloprotease PmbA
B7485_23485316-2.759537hypothetical protein
B7485_23490121-3.628715UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
B7485_23495-116-0.301146fructose 1,6-bisphosphatase
B7485_23500-1170.532766sugar ABC transporter permease YjfF
B7485_23505-2170.061150sugar ABC transporter permease
B7485_235100232.243231ABC transporter ATP-binding protein
B7485_23515-1201.599988inorganic pyrophosphatase
B7485_23520-1181.810186hypothetical protein
B7485_235250130.063435gamma-glutamylcyclotransferase YtfP
B7485_23530316-1.912445translocation and assembly module TamB
B7485_23535217-2.379432outer membrane protein assembly factor
B7485_23540224-3.109756hypothetical protein
B7485_23545124-3.048454peptide-methionine (S)-S-oxide reductase
B7485_23550126-3.556321transporter
B7485_23555127-4.284241hypothetical protein
B7485_23560131-6.263761YtfJ family protein
B7485_23565031-6.331176hypothetical protein
B7485_23570031-7.8958133'(2'),5'-bisphosphate nucleotidase CysQ
B7485_23575232-9.1174952',3'-cyclic-nucleotide 2'-phosphodiesterase
B7485_23580231-8.349133transcriptional regulator
B7485_23585229-7.766883NAD(P)-dependent oxidoreductase
B7485_23590125-5.389062EamA family transporter
B7485_23595026-5.013155iron-sulfur cluster repair protein YtfE
B7485_23600-123-4.514010D-serine/D-alanine/glycine transporter
B7485_23610023-3.510400peptidylprolyl isomerase
B7485_23615224-2.893116peptidylprolyl isomerase
B7485_23620224-3.163416hypothetical protein
B7485_23630326-3.462193TetR family transcriptional regulator
B7485_23635327-2.2934243-ketoacyl-ACP reductase
B7485_23645228-1.339699sugar phosphate isomerase/epimerase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_2385556KDTSANTIGN300.009 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 30.3 bits (68), Expect = 0.009
Identities = 16/59 (27%), Positives = 21/59 (35%), Gaps = 7/59 (11%)

Query: 228 FSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQGLIQTYINFDG 286
FSIY GVG L + I + G G V GVAI ++ +
Sbjct: 454 FSIY-------GGVGAGLGSYTYAKIDNKDVKGYTGMVASGALGVAINAAEGVCVDLEA 505


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23860RTXTOXINA320.003 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 32.2 bits (73), Expect = 0.003
Identities = 15/59 (25%), Positives = 28/59 (47%), Gaps = 4/59 (6%)

Query: 79 TGGIDLSVGAV----MAIAGATTAAMTVAGFSLPIVLLSALGTGILAGLWNGILVAILK 133
TG ID S+ + +++ +AA T + P+ L TGI++G+ A+ +
Sbjct: 361 TGAIDASLTTISTVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASKQAMFE 419


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23965INFPOTNTIATR1652e-53 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 165 bits (418), Expect = 2e-53
Identities = 80/207 (38%), Positives = 115/207 (55%), Gaps = 5/207 (2%)

Query: 3 TPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHRAL 62
+ T + + SY IG +G+ G++ + P+ L G+ D + G + + + L
Sbjct: 24 ATSLTTDKDKLSYSIGADLGKNFKNQGID-INPDVLAKGMQDGMSGAQLILTEEQMKDVL 82

Query: 63 REIHERADAVRRQRFQAMAAE----GVKYLEENAKKEGVNSTESGLQFRVINQGEGAIPA 118
+ + A R F A E G +L N K G+ SGLQ+++I+ G GA P
Sbjct: 83 SKFQKDLMAKRSAEFNKKAEENKAKGDAFLSANKSKPGIVVLPSGLQYKIIDAGTGAKPG 142

Query: 119 RTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIEALTLMPVGSKWELTIPQE 178
++D V V YTG LIDGTVFDS+ G+PA F V+ VIPGW EAL LMP GS WE+ +P +
Sbjct: 143 KSDTVTVEYTGTLIDGTVFDSTEKAGKPATFQVSQVIPGWTEALQLMPAGSTWEVFVPAD 202

Query: 179 LAYGERGAGASIPPFSTLVFEVELLEI 205
LAYG R G I P TL+F++ L+ +
Sbjct: 203 LAYGPRSVGGPIGPNETLIFKIHLISV 229


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23980HTHTETR758e-19 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 75.0 bits (184), Expect = 8e-19
Identities = 39/207 (18%), Positives = 77/207 (37%), Gaps = 20/207 (9%)

Query: 12 KEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSKQTVYHHFGNKENLFIEVLEYTWNDIRQ 71
++ +L A+ F++ G + I KAAG ++ +Y HF +K +LF E+ E + ++I +
Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72

Query: 72 K--EKALDFSDLPPQKAIEKIID-FTWDYYIAN-PWFLKIV-HSENQSKGVH-YAKSQRL 125
E F P E +I ++I+ H + ++QR
Sbjct: 73 LELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRN 132

Query: 126 LEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNINIAALGGYYLINQHTLGLVYHISMV 185
L + +E L + + + + + GY GL+ +
Sbjct: 133 LCL--ESYDRIEQTLKHCIEAKMLPADLMTRRA---AIIMRGYI------SGLMENWLFA 181

Query: 186 --SPQALEARRKVIKETILSWLLVDPS 210
S + R + +L L+ P+
Sbjct: 182 PQSFDLKKEARDYV-AILLEMYLLCPT 207


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23985DHBDHDRGNASE702e-16 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 70.1 bits (171), Expect = 2e-16
Identities = 67/263 (25%), Positives = 115/263 (43%), Gaps = 22/263 (8%)

Query: 7 VAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVEKLHITQQECLAEGVEVICFPAD 66
+A ITGA +GIG+ A+ LA G ++ D + EKL AE FPAD
Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHIAAVDY----NPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 67 VGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRGDLLDLEPDSFDQNIAINTRAPFFL 126
V D + +E+ + + G +D L+N AG+ + G + L + ++ ++N+ F
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVL--RPGLIHSLSDEEWEATFSVNSTGVFNA 123

Query: 127 AQAFSKRLLAQPKPEAELPHRSIIFVSSINAIMLAMNRGEYTIAKTAVSAAARLFAARLC 186
+++ SK ++ + SI+ V S A + + Y +K A + L
Sbjct: 124 SRSVSKYMMDRRSG-------SIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 187 NEQIGVYEVRPGLIKTDM--TIPATAYYDELIAKGL-------VPWGRWGYPADIASTVR 237
I V PG +TDM ++ A E + KG +P + P+DIA V
Sbjct: 177 EYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVL 236

Query: 238 AMAEGKLIYTCGQAVAIDGGLSM 260
+ G+ + + +DGG ++
Sbjct: 237 FLVSGQAGHITMHNLCVDGGATL 259


68B7485_23990B7485_24020Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_23990318-0.3649693-keto-L-gulonate-6-phosphate decarboxylase
B7485_23995424-1.262992L-ribulose-5-phosphate 3-epimerase UlaE
B7485_24000321-1.328089L-ribulose-5-phosphate 4-epimerase UlaF
B7485_24005216-0.182107hypothetical protein
B7485_240102190.44311530S ribosomal protein S6
B7485_240152150.911044primosomal replication protein N
B7485_240202220.03983630S ribosomal protein S18
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24355ECOLNEIPORIN280.034 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 27.8 bits (62), Expect = 0.034
Identities = 6/19 (31%), Positives = 7/19 (36%), Gaps = 2/19 (10%)

Query: 105 FNGDVQI--ELTGYWTWEQ 121
F G + L W EQ
Sbjct: 62 FKGQEDLGNGLKAIWQVEQ 80


69B7485_24170B7485_24270Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_24170-1143.125254phosphoglycerol transferase I
B7485_24180-1142.840989DUF2501 family protein
B7485_24185-1133.372866DNA replication protein DnaC
B7485_24210-1133.256902primosomal protein 1
B7485_24215-2123.357944hypothetical protein
B7485_24220-1132.619082hypothetical protein
B7485_242250133.073287helix-turn-helix transcriptional regulator
B7485_242300152.738574hydroxamate siderophore iron reductase FhuF
B7485_242352191.797073hypothetical protein
B7485_242404261.862133***16S rRNA methyltransferase
B7485_242454231.774645DNA polymerase III subunit psi
B7485_242504242.302515ribosomal-protein-alanine N-acetyltransferase
B7485_242603192.194774noncanonical pyrimidine nucleotidase, YjjG
B7485_242702141.454959peptide chain release factor 3
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_245952FE2SRDCTASE486e-179 Ferric iron reductase signature.
		>2FE2SRDCTASE#Ferric iron reductase signature.

Length = 262

Score = 486 bits (1251), Expect = e-179
Identities = 255/262 (97%), Positives = 255/262 (97%)

Query: 1 MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFIRLDEPAPLNAMTLAQW 60
MAYRSAPLYEDVIWRTHLQPQD LAQAVRA IAKHREHLLEFIRLDEPAPLNAMTLAQW
Sbjct: 1 MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFIRLDEPAPLNAMTLAQW 60

Query: 61 SSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGLMVPPLMLALLTQEKALDV 120
SSPN LSSLLAVYSDHIYRNQP MIRENKPLISLWAQWYIGLMVPPLMLALLTQEKALDV
Sbjct: 61 SSPNVLSSLLAVYSDHIYRNQPMMIRENKPLISLWAQWYIGLMVPPLMLALLTQEKALDV 120

Query: 121 SPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETLISQALVPVVQALEATGEING 180
SPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQ RMETLISQALVPVVQALEATGEING
Sbjct: 121 SPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQHRMETLISQALVPVVQALEATGEING 180

Query: 181 KLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTNGEDNPLWRTVVLRDGLLV 240
KLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTNGEDNPLWRTVVLRDGLLV
Sbjct: 181 KLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKTLTNGEDNPLWRTVVLRDGLLV 240

Query: 241 RRTCCQRYRLPDVQQCGYCTLK 262
RRTCCQRYRLPDVQQCG CTLK
Sbjct: 241 RRTCCQRYRLPDVQQCGDCTLK 262


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24630SACTRNSFRASE554e-12 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 54.6 bits (131), Expect = 4e-12
Identities = 23/80 (28%), Positives = 35/80 (43%), Gaps = 1/80 (1%)

Query: 62 DEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRASNAAAIALYESLGFN 121
A + +IAV DY+++G+G ALL I+ ++ L LE + N +A Y F
Sbjct: 88 GYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI 147

Query: 122 EATIRRNYYPTTDG-REDAI 140
+ Y E AI
Sbjct: 148 IGAVDTMLYSNFPTANEIAI 167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24640TCRTETOQM2144e-64 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 214 bits (546), Expect = 4e-64
Identities = 107/460 (23%), Positives = 207/460 (45%), Gaps = 44/460 (9%)

Query: 12 KRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISIT 71
K +++H DAGKTT+TE +L AI G+V ++D +E+QRGI+I
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSV----DKGTTRTDNTLLERQRGITIQ 57

Query: 72 TSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTR 131
T + F + + VN++DTPGH DF + YR+L+ +D +++I A GV+ +TR L R
Sbjct: 58 TGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 132 LRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFKGVYHLYKDETY 191
P + F+NK+D++ D + +++ +L K +
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVI------------------KQKVE 159

Query: 192 LYQSGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVKGASNEFDKELFLAGEI 251
LY + E + + +DL ++ L + + F +
Sbjct: 160 LYPNMCVTNFTESEQWDTVIEGN------DDLLEKYMSGKSLEALELEQEESIRFHNCSL 213

Query: 252 TPVFFGTALGNFGVDHMLNGLVEWAPAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKH 311
PV+ G+A N G+D+++ + + + + G VFKI+ K
Sbjct: 214 FPVYHGSAKNNIGIDNLIEVITNKFYSS---------THRGQSELCGKVFKIE--YSEK- 261

Query: 312 RDRVAFMRVVSGKYEKGMKLRQVRTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHG 371
R R+A++R+ SG +R K + I++ T + G+ +++AY G+I+ L N
Sbjct: 262 RQRLAYIRLYSGVLHLRDSVRISEKEK-IKITEMYTSINGELCKIDKAYSGEIVILQNEF 320

Query: 372 TIQIGDTFTQGEMMKFTGIPNFA-PELFRRIRLKDPLKQKQLLKGLVQLSEEG-AVQVFR 429
+++ +++ P L + P +++ LL L+++S+ ++ +
Sbjct: 321 -LKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYV 379

Query: 430 PISNNDLIVGAVGVLQFDVVVARLKSEYNVEAVYESVNVA 469
+ +++I+ +G +Q +V A L+ +Y+VE + V
Sbjct: 380 DSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVI 419


70B7485_24345B7485_24495Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_24345-1263.014766protein Smp
B7485_24350-1253.124876phosphoserine phosphatase
B7485_24355-1222.759699DNA repair protein RadA
B7485_24360-2222.244202trifunctional nicotinamide-nucleotide
B7485_243651280.198250energy-dependent translational throttle protein
B7485_24370129-3.172069methionine aminopeptidase
B7485_24375328-1.712667murein transglycosylase
B7485_24380323-2.158967Trp operon repressor
B7485_24385222-2.819895phosphoglycerate mutase GpmB
B7485_24390221-2.708654right origin-binding protein
B7485_24395123-5.695891protein CreA
B7485_24400016-4.271921DNA-binding response regulator
B7485_24405013-4.203570two-component sensor histidine kinase
B7485_24410-112-4.023555cell envelope integrity protein CreD
B7485_24415-114-5.390384two-component system response regulator ArcA
B7485_24420-113-4.669004hypothetical protein
B7485_24425-113-2.857682tRNA/rRNA methyltransferase
B7485_24435-118-0.387160
B7485_244450303.080903
B7485_244501303.218457
B7485_24460-116-1.248117
B7485_24465117-2.271656
B7485_24470016-1.454846
B7485_24475-118-1.093942
B7485_24480-119-1.672872
B7485_24485-315-0.213680
B7485_24490-2182.423816
B7485_244950284.710090
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24710FLGMRINGFLIF300.022 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 29.5 bits (66), Expect = 0.022
Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 2/71 (2%)

Query: 123 QIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRSRVATLK-GADANILQQVRENLP 181
Q+ E AK A V + TE A+ L L+ R A + GA+ + Q++RE
Sbjct: 482 QLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEV-MSQRIREMSD 540

Query: 182 LMPGLTQLVLK 192
P + LV++
Sbjct: 541 NDPRVVALVIR 551


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24720LPSBIOSNTHSS367e-05 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 36.3 bits (84), Expect = 7e-05
Identities = 22/152 (14%), Positives = 54/152 (35%), Gaps = 35/152 (23%)

Query: 71 GKFYPLHTGHIYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQT 130
G F P+ GH+ +I+R C D++++ A+ + +V +RL + +
Sbjct: 7 GSFDPITFGHLDIIERGCRLFDQVYV----------AVLRNPNKQPMFSVQERLEQIAKA 56

Query: 131 FKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKKFMAEKGI---------QPDLIYTSEEA 181
+ N ++ D + + ++ D + A
Sbjct: 57 IAHLPNAQV---------------DSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMA 101

Query: 182 DAPQYMEHLGIETVLVDPKRTFMSISGAQIRE 213
+ + + +ETV + + +S + ++E
Sbjct: 102 NTNKTLAS-DLETVFLTTSTEYSFLSSSLVKE 132


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24750VACCYTOTOXIN290.014 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 29.2 bits (65), Expect = 0.014
Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 4/45 (8%)

Query: 145 PLLVSHGIALGCLVSTILGLPAWAERRLRLRNCSISRVDYQESLW 189
P +V GIA G V T+ GL W ++ N D + +W
Sbjct: 42 PAIVG-GIATGAAVGTVSGLLGWGLKQAEEAN---KTPDKPDKVW 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24765HTHFIS876e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 6e-22
Identities = 33/139 (23%), Positives = 60/139 (43%)

Query: 1 MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKARQQVPDVMILDVGLPDI 60
M T+ + +D+ I L L + G+ V + + D+++ DV +PD
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 SGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYVAKPFSPREVCARVRTLLR 120
+ F+L ++ P LPVL ++A++ + + E GA DY+ KPF E+ + L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 RVKKFSTPSPVIRIGHFEL 139
K+ + L
Sbjct: 121 EPKRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24770PF06580330.003 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.003
Identities = 47/207 (22%), Positives = 80/207 (38%), Gaps = 51/207 (24%)

Query: 298 LTQNARMQAL---------VETL--LRQARLENRQEVVLTAVDVAALFR---RVSEARTV 343
+ Q A++ AL L +R LE+ + ++ L R R S AR V
Sbjct: 157 MAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKAREMLTSLSELMRYSLRYSNARQV 216

Query: 344 QLAE--KNITLHVM--------PTEVNVAAEPALLDQALGNLL-----DNA----IDFTP 384
LA+ + ++ + PA++D + +L +N I P
Sbjct: 217 SLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGIAQLP 276

Query: 385 ESGCITLSAEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSE 444
+ G I L D VTL+V +TGS N ++S+G GL V E
Sbjct: 277 QGGKILLKGTKDNGTVTLEVENTGSLALK----------------NTKESTGTGLQNVRE 320

Query: 445 -VARLFNGEVTLR-NVQEGGVLASLRL 469
+ L+ E ++ + ++G V A + +
Sbjct: 321 RLQMLYGTEAQIKLSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24780HTHFIS824e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 81.8 bits (202), Expect = 4e-20
Identities = 30/122 (24%), Positives = 60/122 (49%), Gaps = 1/122 (0%)

Query: 1 MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGK 60
M IL+ +D+ R L GYDV ++ A + + ++ D +LV+ D+ +P +
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 NGLLLARELRE-QANVALMFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLS 119
N L +++ + ++ ++ ++ ++ + I E GA DY+ KPF+ EL L+
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 120 RT 121

Sbjct: 121 EP 122


71B7485_01725B7485_01755N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_01725-121-2.357033fructokinase
B7485_01730-124-4.495532MFS transporter AraJ
B7485_01735-130-5.599608exonuclease subunit SbcC
B7485_01740-131-6.279818exonuclease sbcCD subunit D
B7485_01750021-1.055263DNA-binding response regulator
B7485_01755221-1.161968PAS domain-containing sensor histidine kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01775ACETATEKNASE290.016 Acetate kinase family signature.
		>ACETATEKNASE#Acetate kinase family signature.

Length = 400

Score = 29.4 bits (66), Expect = 0.016
Identities = 17/69 (24%), Positives = 29/69 (42%), Gaps = 10/69 (14%)

Query: 187 FISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVVNILDP- 245
+G ++D+R L A + D A+LAL + R+ K++ +
Sbjct: 273 VYGISGISSDFRDLEDAAF---------KNGDKRAQLALNVFAYRVKKTIGSYAAAMGGV 323

Query: 246 DVIVLGGGM 254
DVIV G+
Sbjct: 324 DVIVFTAGI 332


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01780TCRTETA531e-09 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 52.5 bits (126), Expect = 1e-09
Identities = 73/356 (20%), Positives = 126/356 (35%), Gaps = 36/356 (10%)

Query: 5 ILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGH---MISYYALVVVVGAPIIALF 61
+ ++AL G+G+ IM VL L ++ S H +++ YAL+ AP++
Sbjct: 11 LSTVALDAVGIGL----IMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 62 SSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSGFPHGAFFGVGAIVLSKIIK 121
S R+ + +LL +A + A+ + +L IGR+V+G GA + I
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIAD--IT 124

Query: 122 PGKVTAAVAGMVSGMTVANLLGIP-LGTYLSQECWRYTFLLIAVFNIAVMASVYFWVPDI 180
G A G +S ++ P LG + F A N + F +P+
Sbjct: 125 DGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPES 184

Query: 181 RDEAKGNLREQ----------FHFLRSPAPWLI--FAATMFGNAGVFAWFSYVKPYMMFI 228
+ LR + + A + F + G W +
Sbjct: 185 HKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG------E 238

Query: 229 SGFSETAMTFIMMLVGLGM---VLGNMLSGRISGRYSPLRIAAVTDFIIVLALLMLFFCG 285
F A T + L G+ + M++G ++ R R + ++L F
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFAT 298

Query: 286 GMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAF--NLGSAVG 339
I + G+ LQ +L + E G G +A +L S VG
Sbjct: 299 RGWMAFPIMVLLASGGIG--MPALQAMLSRQV-DEERQGQLQGSLAALTSLTSIVG 351


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01785RTXTOXIND397e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 39.4 bits (92), Expect = 7e-05
Identities = 34/199 (17%), Positives = 71/199 (35%), Gaps = 14/199 (7%)

Query: 671 QQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALH 730
+ + Q + Q R Q L+ +E + E + +V +
Sbjct: 133 EADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIK 192

Query: 731 SQQQTLQQQDVLAAQSLQKAQAQFDTAL--------QASVFDDQQAFLAALMDEQTLTQL 782
Q T Q Q +L K +A+ T L + V + ++L+ +Q +
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIA-- 250

Query: 783 EQLKQNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQEL-AQTHQKLREN 841
K + Q + V + +Q +Q + L+ + + Q + KLR+
Sbjct: 251 ---KHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQT 307

Query: 842 TTSQGEIRQQLKQDADNRQ 860
T + G + +L ++ + +Q
Sbjct: 308 TDNIGLLTLELAKNEERQQ 326



Score = 39.4 bits (92), Expect = 7e-05
Identities = 25/204 (12%), Positives = 59/204 (28%), Gaps = 18/204 (8%)

Query: 487 EARIKTLEAQRAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEG 546
EA ++ Q + Q ++E + E + +E + L
Sbjct: 133 EADTLKTQSSLLQARLEQ---TRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLT- 188

Query: 547 AALRGQLDALTKQLQRDENEAQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQD 606
+ ++ Q Q + E R + + + + DD L Q
Sbjct: 189 SLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQA 248

Query: 607 -------EHERQL-RLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLP 658
E E + +++ + Q+ +I+ +++ + Q L
Sbjct: 249 IAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLF------KNEILD 302

Query: 659 QEDEEESWLATRQQEAQSWQQRQN 682
+ + + E ++RQ
Sbjct: 303 KLRQTTDNIGLLTLELAKNEERQQ 326



Score = 32.5 bits (74), Expect = 0.009
Identities = 16/150 (10%), Positives = 42/150 (28%), Gaps = 5/150 (3%)

Query: 731 SQQQTLQQQDVLAAQSLQKAQAQFDTA----LQASVFDDQQAFLAALMDEQTLTQLEQLK 786
+ Q + A + Q + L D+ F +E+ L +K
Sbjct: 134 ADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVS-EEEVLRLTSLIK 192

Query: 787 QNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQG 846
+ + Q + A+ + L L + ++
Sbjct: 193 EQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKH 252

Query: 847 EIRQQLKQDADNRQQQQTLMQQIAQMTQQV 876
+ +Q + + + + Q+ Q+ ++
Sbjct: 253 AVLEQENKYVEAVNELRVYKSQLEQIESEI 282


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01790FRAGILYSIN310.009 Fragilysin metallopeptidase (M10C) enterotoxin signat...
		>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin

signature.
Length = 405

Score = 30.8 bits (69), Expect = 0.009
Identities = 14/70 (20%), Positives = 25/70 (35%), Gaps = 4/70 (5%)

Query: 149 KQQHLLAAITDYYQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFP 208
K+ ++ I ++Y + + + I T S+ D + + I A
Sbjct: 135 KEAQMMNEIAEFYAAPFKKTRAINEKEAFECI-YDSRTR--SAGKD-IVSVKINIDKAKK 190

Query: 209 AQNFPPADYI 218
N P DYI
Sbjct: 191 ILNLPECDYI 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01795HTHFIS957e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 95.3 bits (237), Expect = 7e-25
Identities = 33/149 (22%), Positives = 63/149 (42%), Gaps = 9/149 (6%)

Query: 4 RILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQ 63
ILV +D+A IR ++ L + G+ + + + DL++ D ++P +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 FIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRI 123
+ +K+ D+PV++++A+ ++ E GA DY+ KPF EL+ I +
Sbjct: 65 LLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA-- 120

Query: 124 SPMAVEEVIKMQGLSLDPTSHRVMAGEEP 152
E + L D + G
Sbjct: 121 -----EPKRRPSKLEDDSQDGMPLVGRSA 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_01800PF06580340.001 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 34.1 bits (78), Expect = 0.001
Identities = 19/105 (18%), Positives = 33/105 (31%), Gaps = 26/105 (24%)

Query: 325 LVYNAVNH----TPEGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKAR 380
LV N + H P+G I ++ + VE+ G
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKN---------------- 306

Query: 381 SRQTGGSGLGLAIVKHAVNH---HESRLNIESTVGKGTRFSFVIP 422
+G GL V+ + E+++ + GK +IP
Sbjct: 307 --TKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM-VLIP 348


72B7485_01985B7485_02030N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_019853240.829495AmpG family muropeptide MFS transporter
B7485_019900171.482779hypothetical protein
B7485_01995-1180.890062protein BolA
B7485_02000-1160.560709hypothetical protein
B7485_02005015-0.163791trigger factor
B7485_02015121-0.045663ATP-dependent Clp protease proteolytic subunit
B7485_02020330-0.893878ATP-dependent Clp protease ATP-binding subunit
B7485_02025426-0.357506endopeptidase La
B7485_02030328-0.272955DNA-binding protein HU-beta
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02015TCRTETA393e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 39.0 bits (91), Expect = 3e-05
Identities = 71/347 (20%), Positives = 135/347 (38%), Gaps = 20/347 (5%)

Query: 62 KFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLEPGTQLRWMAALAVVIAFCS 121
+F +P++ + F RR LL + V A M W+ + ++A +
Sbjct: 56 QFACAPVLGALSDRF--GRRPVLLVSLAGAAVDYAIMAT----APFLWVLYIGRIVAGIT 109

Query: 122 ASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSGGLALWLADKWLGWQGMYWLMA 181
+ V A+ D+ +ER + GM+ L + ++ A
Sbjct: 110 GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGG--FSPHAPFFAAA 167

Query: 182 AL-LIPCIIATLLAPEP--TDTIPVPKTLEQAVVAPLRDFFGRNNAWLILLLIVLYKLGD 238
AL + + L PE + P+ + + + A L+ + ++ +G
Sbjct: 168 ALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQ 227

Query: 239 AFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGALYGGILMQRLSLFRALLIFGIL 298
A +L F +DA +G+ G+L ++ A+ G + RL RAL+ G++
Sbjct: 228 VPA-ALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM-LGMI 285

Query: 299 QGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGTSAFVALLMTLCNKSFSATQFALLS 358
A GY LL+ + + V GG+G A A+L ++ L+
Sbjct: 286 --ADGTGYILLAFATRGWMAFPIMVLL--ASGGIGMPALQAMLSRQVDEERQGQLQGSLA 341

Query: 359 ALSAVGRVYVGPVAGWFVEAHGWSTF--YLFSVAAAVPGLILLLVCR 403
AL+++ + VGP+ + A +T+ + + AA+ L L + R
Sbjct: 342 ALTSLTSI-VGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02020PF06291270.030 Lambda prophage Bor protein
		>PF06291#Lambda prophage Bor protein

Length = 102

Score = 26.5 bits (58), Expect = 0.030
Identities = 11/34 (32%), Positives = 18/34 (52%)

Query: 3 KKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQ 36
KK+LF ++ GCA+ T+ PT P++
Sbjct: 7 KKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKE 40


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02045HTHFIS290.043 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.043
Identities = 16/73 (21%), Positives = 29/73 (39%), Gaps = 13/73 (17%)

Query: 60 ERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIG 119
E P+ E + ++G+ A + +Y RL D +++ G
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQ----EIYRVLARLMQTD---------LTLMITG 167

Query: 120 PTGSGKTLLAETL 132
+G+GK L+A L
Sbjct: 168 ESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02050GPOSANCHOR350.001 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 35.0 bits (80), Expect = 0.001
Identities = 34/133 (25%), Positives = 69/133 (51%), Gaps = 15/133 (11%)

Query: 191 ERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQREYYLNEQMKAIQKELGEMDDVPD- 249
LE A +E + +L R +++ ++ S+ +Q++A ++L E + + +
Sbjct: 291 AALEAEKADLEHQSQVLNAN---RQSLRRDLDASREAK---KQLEAEHQKLEEQNKISEA 344

Query: 250 ENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSPMS-AEATVVRGYIDWMVQVPWNARSK 308
++L+R +DA++ EAK++ EAE QKL+ + +S A +R +D + A+ +
Sbjct: 345 SRQSLRRDLDASR---EAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASRE----AKKQ 397

Query: 309 VKKDLRQAQEILD 321
V+K L +A L
Sbjct: 398 VEKALEEANSKLA 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02055DNABINDINGHU1173e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 117 bits (294), Expect = 3e-38
Identities = 49/88 (55%), Positives = 67/88 (76%)

Query: 2 NKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVALVGFGTFAVKERAARTGR 61
NK LI K+A +++K + A+DA+ ++V+ L +G+ V L+GFG F V+ERAAR GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 62 NPQTGKEITIAAAKVPSFRAGKALKDAV 89
NPQTG+EI I A+KVP+F+AGKALKDAV
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90


73B7485_02115B7485_02170N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_02115014-0.139314cyclic diguanylate phosphodiesterase
B7485_02120015-0.750349hypothetical protein
B7485_02125118-1.531058maltose O-acetyltransferase
B7485_02130119-3.680173hemolysin expression-modulating protein Hha
B7485_02140119-5.122025Hha toxicity attenuator
B7485_02150218-0.382502multidrug efflux RND transporter permease
B7485_02155116-0.692196MexE family multidrug efflux RND transporter
B7485_02160218-0.571250transcriptional regulator
B7485_02165116-0.062770hypothetical protein
B7485_021701170.318449mechanosensitive channel MscK
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02145BCTERIALGSPF300.024 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 29.8 bits (67), Expect = 0.024
Identities = 31/137 (22%), Positives = 54/137 (39%), Gaps = 24/137 (17%)

Query: 245 IWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLANGKIVGAEA 304
W+ L L+ G +A +LR R+ + + P++ G+I
Sbjct: 228 PWMLLALLAGFMAFRVMLR------QEKRRVS-----FHRRLLHLPLI----GRIARGLN 272

Query: 305 LARWPQTDGSWLSPDSFIPLAQQTGLS-EPLTLLIIRSVFEDMGDCLRQHPQQHISINLE 363
AR+ +T + S +PL Q +S + ++ R D +R+ H + LE
Sbjct: 273 TARYARTLSILNA--SAVPLLQAMRISGDVMSNDYARHRLSLATDAVREGVSLHKA--LE 328

Query: 364 STVLTSEKIPQLLREMI 380
T L P ++R MI
Sbjct: 329 QTAL----FPPMMRHMI 341


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02170ACRIFLAVINRP13660.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1366 bits (3536), Expect = 0.0
Identities = 800/1033 (77%), Positives = 913/1033 (88%), Gaps = 1/1033 (0%)

Query: 1 MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVTISASYPGADAKTVQDT 60
M NFFI RPIFAWV+AII+M+AG LAIL+LPVAQYPTIAPPAV++SA+YPGADA+TVQDT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADIAQVQVQNKLQLAMPLLPQ 120
VTQVIEQNMNGIDNLMYMSS SDS G+V ITLTF+SGTD DIAQVQVQNKLQLA PLLPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 EVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAANMKDAISRTSGVGDVQLFGS 180
EVQQQG+SVEKSSSS+LMV G ++ + TQ+DISDYVA+N+KD +SR +GVGDVQLFG+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRL 240
QYAMRIW++ + LNK++LTPVDVI +K QN Q+AAGQLGGTP + GQQLNASIIAQTR
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 TSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYDIIAEFNGQPASGLGIKLATGANAL 300
+ EEFGK+ L+VN DGS V L+DVA++ELGGENY++IA NG+PA+GLGIKLATGANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 DTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQ 360
DTA AI+A+LA+++PFFP G+K++YPYDTTPFV++SIHEVVKTL EAI+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420
N RATLIPTIAVPVVLLGTFA+LAAFG+SINTLTMFGMVLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 AEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGSTGAIYRQFSITIVSAMAL 480
E+ LPPKEAT KSM QIQGALVGIAMVLSAVF+PMAFFGGSTGAIYRQFSITIVSAMAL
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATMLKPIAKGDHGEGKKGFFGWLNRMFEKSTHHYTDSVGGILRSTGR 540
SVLVALILTPALCAT+LKP++ H E K GFFGW N F+ S +HYT+SVG IL STGR
Sbjct: 481 SVLVALILTPALCATLLKPVSAE-HHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 541 YLVLYLIIVVGMAYLFVRLPSSFLPDEDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLT 600
YL++Y +IV GM LF+RLPSSFLP+EDQGVF+TM+QLPAGATQERTQKVL++VT YYL
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 601 KEKNNVESVFAVNGFGFAGRGQNTGIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKD 660
EK NVESVF VNGF F+G+ QN G+AFVSLK W +R G+EN EA+ RA +I+D
Sbjct: 600 NEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRD 659

Query: 661 AMVFAFNLPAIVELGTATGFDFELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNG 720
V FN+PAIVELGTATGFDFELIDQAGLGH+ LTQARNQLL AA+HP L SVRPNG
Sbjct: 660 GFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNG 719

Query: 721 LEDTPQFKIDIDQEKAQALGVSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYR 780
LEDT QFK+++DQEKAQALGVS++DIN T+ A GG+YVNDFIDRGRVKK+YV ++AK+R
Sbjct: 720 LEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFR 779

Query: 781 MLPDDIGDWYVRAADGQMVPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEA 840
MLP+D+ YVR+A+G+MVPFSAF++S W YGSPRLERYNGLPSMEI G+AAPG S+G+A
Sbjct: 780 MLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDA 839

Query: 841 MELMEQLASKLPTGVGYDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFS 900
M LME LASKLP G+GYDWTGMSYQERLSGNQAP+L AIS +VVFLCLAALYESWS P S
Sbjct: 840 MALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 901 VMLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGL 960
VMLVVPLG++G LLAAT NDVYF VGLLTTIGLSAKNAILIVEFAKDLM+KEGKG+
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGV 959

Query: 961 IEATLDAVRMRLRPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIF 1020
+EATL AVRMRLRPILMTSLAFILGV+PL IS GAGSGAQNAVG GVMGGMV+AT+LAIF
Sbjct: 960 VEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIF 1019

Query: 1021 FVPVFFVVVRRRF 1033
FVPVFFVV+RR F
Sbjct: 1020 FVPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02175RTXTOXIND447e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.0 bits (104), Expect = 7e-07
Identities = 33/212 (15%), Positives = 71/212 (33%), Gaps = 23/212 (10%)

Query: 100 TYQATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTA 159
+ Y A +L + + Q+ Q +++ ++ L +Q +
Sbjct: 256 EQENKYVEAVNELR--VYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGL 313

Query: 160 AKAAVETARINLAYTKVTSPISGRIGKSNV-TEGALVQNGQATALATVQQLDPIYVDVTQ 218
+ + + +P+S ++ + V TEG +V + T + V + D + V
Sbjct: 314 LTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAE-TLMVIVPEDDTLEVTALV 372

Query: 219 SSNDFLRLKQELA----------NGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVD 268
+ D + KV I D I+ + G + ++++
Sbjct: 373 QNKDIGFINVGQNAIIKVEAFPYTRYGYLV---GKVKNINLDAIEDQRLGLVFNVIISIE 429

Query: 269 QTTGSITLRAIFPNPDHTLLPGMFVRARLEEG 300
+ S + I L GM V A ++ G
Sbjct: 430 ENCLSTGNKNIP------LSSGMAVTAEIKTG 455



Score = 32.9 bits (75), Expect = 0.002
Identities = 26/127 (20%), Positives = 50/127 (39%), Gaps = 10/127 (7%)

Query: 49 PLQITTELPGR-TSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQATYDS 107
++I G+ T + R E++P + I+ + KEG + G L ++ +A
Sbjct: 79 QVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA---- 134

Query: 108 AKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAAKAAVETA 167
D K Q++ A+L RYQ L + I + + V+ + T+
Sbjct: 135 ---DTLKTQSSLLQARLEQTRYQILSRS--IELNKLPELKLPDEPYFQNVSEEEVLRLTS 189

Query: 168 RINLAYT 174
I ++
Sbjct: 190 LIKEQFS 196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02180HTHTETR2225e-76 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 222 bits (567), Expect = 5e-76
Identities = 215/215 (100%), Positives = 215/215 (100%)

Query: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60
MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120
EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180
GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE 215
APQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE
Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02190RTXTOXIND320.017 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.7 bits (72), Expect = 0.017
Identities = 19/125 (15%), Positives = 40/125 (32%), Gaps = 6/125 (4%)

Query: 28 QNTAFARASSNGDLPTKADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEE 87
N RA L + + L L+ + A L++ ++ E
Sbjct: 207 LNLDKKRAERLTVLARINRYENLSRVEKSR--LDDFSSLLHKQAIAKHAVLEQENKYVEA 264

Query: 88 TVQLRQKVAEAPEKMRQATAALTALSDVDND--EETRKIL--STLSLRQLETRVAQALDD 143
+LR ++ + + +A V E L +T ++ L +A+ +
Sbjct: 265 VNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEER 324

Query: 144 LQNAQ 148
Q +
Sbjct: 325 QQASV 329


74B7485_02660B7485_02690N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_02660229-2.436057MFS transporter
B7485_02670014-0.619971Fe2+-enterobactin ABC transporter
B7485_02675-114-0.3868842,3-dihydroxybenzoate-AMP ligase
B7485_02680-2122.962718enterobactin synthase component B
B7485_02690-2102.8701852,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02740TCRTETA362e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 36.3 bits (84), Expect = 2e-04
Identities = 82/394 (20%), Positives = 145/394 (36%), Gaps = 44/394 (11%)

Query: 27 FISIVSLGLLGVAVPVQIQMMTHSTWQV---GLSVTLTGGAMFVGLMVGGVLADRYERKK 83
+ V +GL+ +P ++ + HS G+ + L F V G L+DR+ R+
Sbjct: 15 ALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRP 74

Query: 84 VILLARGTCGIGFIGLCLNALL--PEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGR 141
V+L + G ++ + P L +Y+ + G + G A A + +
Sbjct: 75 VLL-------VSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVA-GAYIADITDG 126

Query: 142 ENLMQAGAITMLTVRLGSVISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPP 201
+ + G V P++GGL+ GG + + AA L L LP
Sbjct: 127 DERARHFGFMSACFGFGMVAGPVLGGLM---GGFSPHAPFFAAAALNGLNFLTGCFLLPE 183

Query: 202 PPQPLEHPLK----SLLAGFRFLLASPLLGGLLTMA----------SAVLVLYPALADNW 247
+ PL+ + LA FR+ ++ L+ + +A+ V++ D +
Sbjct: 184 SHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFG--EDRF 241

Query: 248 QMSAAQIGFLYAAIP-LGAAIGALTSGKLAHSARPGLLMLLSTLGS---FLAIGLFGLMP 303
A IG AA L + A+ +G +A ++L + ++ +
Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGW 301

Query: 304 MWILGVVCLALFGWLSAVSSLLQYTMLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGG 363
M +V LA G ML Q E G++ G A +G L
Sbjct: 302 MAFPIMVLLASGGIGMPALQ----AMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTA 357

Query: 364 LGAMMTPVASASASGFGLLIIGVLLLLVLVELRR 397
+ A + + +G+ + L LL L LRR
Sbjct: 358 IYA----ASITTWNGWAWIAGAALYLLCLPALRR 387


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02745FERRIBNDNGPP632e-13 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 63.0 bits (153), Expect = 2e-13
Identities = 61/285 (21%), Positives = 102/285 (35%), Gaps = 35/285 (12%)

Query: 40 HTLESQPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQ 99
H P RIV+ LLA+ VAD + R W E L
Sbjct: 29 HAAAIDPNRIVALEWLPVELLLALGIVPYG---------VADTINY-RLW---VSEPPLP 75

Query: 100 RLYIG-----EPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKS--- 151
I EP+ E + P ++ SA G S + L+ IAP N+ D
Sbjct: 76 DSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPS----PEMLARIAPGRGFNFSDGKQPL 131

Query: 152 --WQSLLTQLGEITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLW 209
+ LT++ ++ + A +AQ++ + + K + + ++
Sbjct: 132 AMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVF 191

Query: 210 TPESAQGQMLEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQ 269
P S ++L++ G NA Q + + + LAA + + L +
Sbjct: 192 GPNSLFQEILDEYGIP--------NAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNS 243

Query: 270 KDADAIYANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERL 314
KD DA+ A PL +P V+ + + F SAM + L
Sbjct: 244 KDMDALMATPLWQAMPFVRAGRFQRVPAVWFYGATLSAMHFVRVL 288


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02760ISCHRISMTASE444e-161 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 444 bits (1142), Expect = e-161
Identities = 146/299 (48%), Positives = 195/299 (65%), Gaps = 18/299 (6%)

Query: 1 MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANI 60
MAIP +Q Y +P + D+PQNKV W +P RA LLIHDMQ+YFV + + ++ ANI
Sbjct: 1 MAIPAIQPYQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANI 60

Query: 61 AALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTV 120
L++ C Q IPV YTAQP Q+ +DRALL D WGPGL P ++K++ L P+ DD V
Sbjct: 61 RKLKNQCVQLGIPVVYTAQPGSQNPDDRALLTDFWGPGLNSGPYEEKIITELAPEDDDLV 120

Query: 121 LVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD 180
L KWRYSAF R+ L +M+++ GR+QLIITG+YAHIGC+ TA +AFM DIK F V DA+AD
Sbjct: 121 LTKWRYSAFKRTNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD 180

Query: 181 FSRDEHLMSLKYVAGRSGRVVMTEELL------PAPIPASKA-----------ALREVIL 223
FS ++H M+L+Y AGR VMT+ LL PA + + A +R+ I
Sbjct: 181 FSLEKHQMALEYAAGRCAFTVMTDSLLDQLQNAPADVQKTSANTGKKNVFTCENIRKQIA 240

Query: 224 PLLDESDEPFDDD-NLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLS 281
LL E+ E D +L+D GLDSVR+M L +WR+ ++ FV LA+ PTI+ W KLL+
Sbjct: 241 ELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLT 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_02765DHBDHDRGNASE353e-126 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 353 bits (907), Expect = e-126
Identities = 106/258 (41%), Positives = 147/258 (56%), Gaps = 20/258 (7%)

Query: 5 GKNVWVTGAGKGIGYATALAFVEAGAKVTGFD---------------QAFTQEQYPFATE 49
GK ++TGA +GIG A A GA + D +A E +P
Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP---- 63

Query: 50 VMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAVNVGGAFN 109
DV D+ + ++ R+ E +D+L+N AG+LR G LS E+W+ TF+VN G FN
Sbjct: 64 -ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122

Query: 110 LFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGLELAGSGVRC 169
+ +R G+IVTV S+ A R M+AY +SKAA +GLELA +RC
Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182

Query: 170 NVVSLGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEIANTILFLASDL 229
N+VS GST+TDMQ +LW ++ EQ I+G E FK GIPL K+A+P +IA+ +LFL S
Sbjct: 183 NIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242

Query: 230 ASHITLQDIVVDGGSTLG 247
A HIT+ ++ VDGG+TLG
Sbjct: 243 AGHITMHNLCVDGGATLG 260


75B7485_04375B7485_04405N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_04375-1162.300590inner membrane transport permease YbhR
B7485_04385117-0.821544ABC transporter permease
B7485_04390117-0.606564multidrug ABC transporter ATP-binding protein
B7485_04395016-0.236463transporter
B7485_044000150.113876transcriptional regulator
B7485_04405016-0.224178ATP-dependent RNA helicase RhlE
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04435ABC2TRNSPORT473e-08 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 47.2 bits (112), Expect = 3e-08
Identities = 36/146 (24%), Positives = 63/146 (43%), Gaps = 5/146 (3%)

Query: 197 AREREQGTLDQLLVSPLTTWQIFIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALF 256
R Q T + +L + L I +G+ A A IG+ A + + L+L
Sbjct: 92 GRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGA---GIGVVAAALGYTQWLSLL 148

Query: 257 YFTMVI--YGLSLVGFGLLISSLCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQN 314
Y VI GL+ G+++++L + + + P + LSG V PV+ +P+ Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 315 LTWINPIRHFTDITKQIYLKDASLDI 340
P+ H D+ + I L +D+
Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPVVDV 234


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04445PF05272310.012 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 31.2 bits (70), Expect = 0.012
Identities = 20/90 (22%), Positives = 28/90 (31%), Gaps = 21/90 (23%)

Query: 293 TPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKELTKKFGDFAATDHVNFAV 352
PR E + +LG P + + + K HV +
Sbjct: 547 VPRLEKWLVHVLGKTPDDYKP-------------RRLRYLQLVGKYI----LMGHVARVM 589

Query: 353 KRGEIFG----LLGPNGAGKSTTFKMMCGL 378
+ G F L G G GKST + GL
Sbjct: 590 EPGCKFDYSVVLEGTGGIGKSTLINTLVGL 619



Score = 29.3 bits (65), Expect = 0.047
Identities = 11/23 (47%), Positives = 13/23 (56%)

Query: 34 YVTGLVGPDGAGKTTLMRMLAGL 56
Y L G G GK+TL+ L GL
Sbjct: 597 YSVVLEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04450RTXTOXIND636e-13 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 62.5 bits (152), Expect = 6e-13
Identities = 42/259 (16%), Positives = 92/259 (35%), Gaps = 25/259 (9%)

Query: 83 ALMQAKAGVSVAQAQYDLMLAGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRT 142
Q + + +A+ +LA E + + + + L +
Sbjct: 201 QKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENK 260

Query: 143 ISA--NDLENARSSRDQAQATLKSAQDKLRQYRSGNREQ---DIAQAKASLEQAQAQLAQ 197
N+L +S +Q ++ + SA+++ + + + + Q ++ +LA+
Sbjct: 261 YVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAK 320

Query: 198 AELNLQDSTLIAPSDGTLLTRAV-EPGTVLNEGGTVFTVSLT-RPVWVRAYVDERNLDQA 255
E Q S + AP + V G V+ T+ + + V A V +++
Sbjct: 321 NEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFI 380

Query: 256 QPGRKVLLYTDGRPDKPYH---GQIGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVT--- 309
G+ ++ + P Y G++ ++ A D R LV+ + I +
Sbjct: 381 NVGQNAIIKVEAFPYTRYGYLVGKVKNINLDA--------IEDQRLGLVFNVIISIEENC 432

Query: 310 ----DADDALRQGMPVTVQ 324
+ + L GM VT +
Sbjct: 433 LSTGNKNIPLSSGMAVTAE 451


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04455HTHTETR737e-18 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 72.7 bits (178), Expect = 7e-18
Identities = 33/214 (15%), Positives = 77/214 (35%), Gaps = 17/214 (7%)

Query: 9 KGEQAKKQLIAAALAQFGEYGMNATT-REIAAQAGQNIAAITYYFGSKEDLYLACAQWIA 67
+ ++ ++ ++ AL F + G+++T+ EIA AG AI ++F K DL+ +
Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67

Query: 68 DFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRACRNMIKLLTQDDTVNLSKFISREQL 127
IGE E + P + +RE+++ + + + + + F E +
Sbjct: 68 SNIGELEL---EYQAKFPGDP---LSVLREILIHVLESTVTEERRRLLMEII-FHKCEFV 120

Query: 128 SPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDANDTRMILHTHALIGEILAFRLGKETIL 187
A + + + + + +A L T + + G
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKH--CIEAKMLPADLMTRRAAIIMRGYISG----- 173

Query: 188 LRTGWTAFDEEKTELINQTVTCHIDLILQGLSQR 221
L W + + + ++ ++L+
Sbjct: 174 LMENWLFAPQSFD--LKKEARDYVAILLEMYLLC 205


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04460SECA310.014 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 30.6 bits (69), Expect = 0.014
Identities = 20/67 (29%), Positives = 34/67 (50%), Gaps = 4/67 (5%)

Query: 246 QQVLVFTRTKHGANHLAEQLNKDGIRSVAIHG-NKSQGARTRALADFKSGDIRVLVATDI 304
Q VLV T + + ++ +L K GI+ ++ + A A A + + V +AT++
Sbjct: 450 QPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAA---VTIATNM 506

Query: 305 AARGLDI 311
A RG DI
Sbjct: 507 AGRGTDI 513


76B7485_04675B7485_04710N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_04675011-0.125515multidrug transporter MdfA
B7485_04680115-1.005656hypothetical protein
B7485_04690-113-1.283025sugar-phosphatase
B7485_04700-214-0.700150MFS transporter
B7485_04705-216-0.747171DNA-binding transcriptional regulator
B7485_04710117-1.456800transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04725TCRTETB393e-05 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 38.7 bits (90), Expect = 3e-05
Identities = 28/155 (18%), Positives = 61/155 (39%), Gaps = 5/155 (3%)

Query: 48 QAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQ 107
A +WV T+ + G + G LSD++G + ++L G++ + + +
Sbjct: 48 PASTNWVNTAFMLTFSIGTAV---YGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS 104

Query: 108 FTLL-RFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIH 166
++ RF+QG A+ + + K L+ ++ + +GP +G H
Sbjct: 105 LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAH 164

Query: 167 VLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGE 201
+ W +L + I+ L + + + G
Sbjct: 165 YIHW-SYLLLIPMITIITVPFLMKLLKKEVRIKGH 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04740PF05272330.002 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 33.1 bits (75), Expect = 0.002
Identities = 24/61 (39%), Positives = 29/61 (47%), Gaps = 1/61 (1%)

Query: 302 DSAWVAGVSVVLWGLGASLGFPLTISAASDTGPDAPTRVSVVATTGYLAFLVGPPLLGYL 361
D +W+AG +VVLW SL LT DT PD R ++A YL F P L
Sbjct: 270 DWSWLAGCTVVLWPDCDSLREKLTRQELKDT-PDPLAREKLLAAKPYLPFDKQPGQKAML 328

Query: 362 G 362
G
Sbjct: 329 G 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04745HTHTETR506e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 49.6 bits (118), Expect = 6e-10
Identities = 14/83 (16%), Positives = 30/83 (36%), Gaps = 4/83 (4%)

Query: 2 RRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGSMTYYFSGIDELLLEAFS 61
+ + R+ I+ L G+ + + +IA AGV G++ ++F +L E +
Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW- 63

Query: 62 SFTEIMSRQYQAFFSDVSDAQGA 84
E+ +
Sbjct: 64 ---ELSESNIGELELEYQAKFPG 83


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04750TCRTETA300.020 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 30.2 bits (68), Expect = 0.020
Identities = 20/106 (18%), Positives = 34/106 (32%), Gaps = 6/106 (5%)

Query: 394 LMIGMITFQFSTFSFGMGNAAGLLFAGIML-GFMRANHPTFG-YIPQ--GALSMVKEFGL 449
L++ + +L+ G ++ G A G YI + FG
Sbjct: 76 LLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGF 135

Query: 450 IVFMAGVGLSAGSGINNGLGAIGGQM--LIAGLIVSLVPVVICFLF 493
+ G G+ AG + +G A + L + CFL
Sbjct: 136 MSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL 181


77B7485_04800B7485_04845N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_04800-2170.296257arginine ABC transporter ATP-binding protein
B7485_04805-2170.961357lipoprotein
B7485_04810017-0.177036hypothetical protein
B7485_04820017-0.591258pentapeptide repeat protein
B7485_04825219-0.079801hypothetical protein
B7485_04830019-7.441075N-acetylmuramoyl-L-alanine amidase
B7485_04835024-9.459009hypothetical protein
B7485_04845126-8.820330NAD(P)-dependent oxidoreductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04840PF05272300.010 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.0 bits (67), Expect = 0.010
Identities = 9/18 (50%), Positives = 12/18 (66%)

Query: 31 LVLLGPSGAGKSSLLRVL 48
+VL G G GKS+L+ L
Sbjct: 599 VVLEGTGGIGKSTLINTL 616


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04870ECOLIPORIN300.010 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 29.9 bits (67), Expect = 0.010
Identities = 21/54 (38%), Positives = 27/54 (50%), Gaps = 9/54 (16%)

Query: 2 RRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRIKVLVIHYTADD 55
R+V LV ALL AG A I K+G +LD Y ++ L HY +DD
Sbjct: 3 RKVLALVIPALLAAGAAHAAEIYNKDGNKLDL-------YGKVDGL--HYFSDD 47


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04875NUCEPIMERASE738e-17 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 73.3 bits (180), Expect = 8e-17
Identities = 70/363 (19%), Positives = 123/363 (33%), Gaps = 65/363 (17%)

Query: 1 MKVLVTGATSGLGRNAVEFLCQKGISVRA---------SGRNEAMGKLLEKMGAEFVPTD 51
MK LVTGA +G + + L + G V +A +LL + G +F D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 52 LTELVSSQAKVMLAGIDTLWHCS-------SFTSPWGTQQAFDLANVRATRRLGEWAVAW 104
L + + ++ S +P A+ +N+ + E
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPH----AYADSNLTGFLNILEGCRHN 116

Query: 105 GVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQTRFT 164
+++ ++ SS S+Y + D + +A +K A+E + + S T
Sbjct: 117 KIQHLLYASSSSVYGL-NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSH-LYGLPAT 174

Query: 165 ILRPQSLFGPHDK--VFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLASQEA 222
LR +++GP + + + + M SI + + G D TY ++ A+
Sbjct: 175 GLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVI 234

Query: 223 CDKLPS--------------GRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLD 268
RVYNI N L +Q L D L I+ + +P D
Sbjct: 235 PHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGD 294

Query: 269 MIARSMERLGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAW 328
+ T D E +G+ P T+ +G++ W
Sbjct: 295 V----------------LETS-----------ADTKALYEVIGFTPETTVKDGVKNFVNW 327

Query: 329 LRD 331
RD
Sbjct: 328 YRD 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_04880NUCEPIMERASE562e-10 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 55.6 bits (134), Expect = 2e-10
Identities = 29/125 (23%), Positives = 52/125 (41%), Gaps = 17/125 (13%)

Query: 4 RILVLGASGYIGQHLVRTLSQQGHQILA---------AARHVDRLAKLQLANVSCHKVDL 54
+ LV GA+G+IG H+ + L + GHQ++ + RL L HK+DL
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 55 SWPDNLPALLQD--IDTVYFLVH------SMGEGGDFIAQERQLALNVRDALREVPVKQL 106
+ + + L + V+ H S+ + LN+ + R ++ L
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQHL 121

Query: 107 IFLSS 111
++ SS
Sbjct: 122 LYASS 126


78B7485_05985B7485_06025N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_05985325-1.918763flagellar hook protein FlgE
B7485_05990020-1.571655flagellar basal-body rod protein FlgG
B7485_05995018-2.988311flagellar L-ring protein
B7485_06000-214-2.941494flagellar P-ring protein
B7485_06010216-1.354538flagellar assembly peptidoglycan hydrolase FlgJ
B7485_06015116-2.298567flagellar hook-filament junction protein FlgL
B7485_06025018-0.866716ribonuclease E
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06120FLGHOOKAP1393e-05 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 39.2 bits (91), Expect = 3e-05
Identities = 16/49 (32%), Positives = 28/49 (57%)

Query: 354 TLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR 402
L+N S V+L +E N+ Q+ Y +NAQ ++T + I + +N+R
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 36.9 bits (85), Expect = 1e-04
Identities = 22/56 (39%), Positives = 30/56 (53%), Gaps = 4/56 (7%)

Query: 6 AVSGLNAAATNLDVIGNNIANSATYGFKSGTASFAD----MFAGSKVGLGVKVAGI 57
A+SGLNAA L+ NNI++ G+ T A + AG VG GV V+G+
Sbjct: 7 AMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGV 62


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06130FLGHOOKAP1444e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 43.8 bits (103), Expect = 4e-07
Identities = 18/81 (22%), Positives = 36/81 (44%), Gaps = 14/81 (17%)

Query: 3 SSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDLLYQTIRQPGAQSSEQTTL 62
S + A +GL+A Q ++ +NN+++ + G+ RQ + + +TL
Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI--------------MAQANSTL 47

Query: 63 PSGLQIGTGVRPVATERLHSQ 83
+G +G GV +R +
Sbjct: 48 GAGGWVGNGVYVSGVQREYDA 68



Score = 41.1 bits (96), Expect = 3e-06
Identities = 11/41 (26%), Positives = 21/41 (51%)

Query: 220 ETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL 260
S VN+ EE N+ + Q+ Y N++ + T + + L +
Sbjct: 505 SISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06135FLGLRINGFLGH349e-126 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 349 bits (897), Expect = e-126
Identities = 232/232 (100%), Positives = 232/232 (100%)

Query: 1 MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY 60
MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY
Sbjct: 1 MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINY 60

Query: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA 120
GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA
Sbjct: 61 GYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNA 120

Query: 121 RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF 180
RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF
Sbjct: 121 RADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRF 180

Query: 181 SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232
SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM
Sbjct: 181 SGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSPM 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06140FLGPRINGFLGI427e-152 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 427 bits (1100), Expect = e-152
Identities = 157/363 (43%), Positives = 213/363 (58%), Gaps = 9/363 (2%)

Query: 4 FLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVGLDGTGDQTTQTPFTTQTLN 63
F + L A RI+D+ S+Q R N LIGYGLVVGL GTGD +PFT Q++
Sbjct: 13 FSALPFLSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMR 72

Query: 64 NMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQTIDVVVSSMGNAKSLRGGTLLM 123
ML LGIT G + KN+AAVMVTA+LPPF G +DV VSS+G+A SLRGG L+M
Sbjct: 73 AMLQNLGITTQGGQS-NAKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIM 131

Query: 124 TPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQLNGGRITNGAVIERELPSQFGVGN 183
T L G D Q+YA+AQG ++V G A +++ R+ NGA+IERELPS+F
Sbjct: 132 TSLSGADGQIYAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSV 191

Query: 184 TLNLQLNDEDFSMAQQIADTINRVR----GYGSATALDARTIQVRVPSGNSSQVRFLADI 239
L LQL + DFS A ++AD +N G A D++ I V+ P + R +A+I
Sbjct: 192 NLVLQLRNPDFSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPRV-ADLTRLMAEI 250

Query: 240 QNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQANVSQPDTPFGG 299
+N+ V T AKVVIN RTG++V+ +V + AV+ G L+V V V QP PF
Sbjct: 251 ENLTVE-TDTPAKVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQP-APFSR 308

Query: 300 GQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRALNALGATPMDLMSILQSMQSAGCLR 359
GQT V PQT I Q G + ++ L +V LN++G +++ILQ ++SAG L+
Sbjct: 309 GQTAVQPQTDIMAMQEGSKV-AIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQ 367

Query: 360 AKL 362
A+L
Sbjct: 368 AEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06145FLGFLGJ5030.0 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 503 bits (1297), Expect = 0.0
Identities = 308/313 (98%), Positives = 309/313 (98%)

Query: 1 MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG 60
MISDSKLLASAAWDAQSLNELKAKA EDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG
Sbjct: 1 MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDG 60

Query: 61 LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET 120
LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET
Sbjct: 61 LFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLET 120

Query: 121 VVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPAQLASQQSGVPHHLILAQAAL 180
VVRYQNQ LSQLVQKAVPRNYDDSLPGDS+AFLAQLSLPAQLASQQSGVPHHLILAQAAL
Sbjct: 121 VVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQAAL 180

Query: 181 ESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITTTEYENGEAKKVKAKFRVYSSYL 240
ESGWGQRQIRRENGEPSYNLFGVKASGNWKG VTEITTTEYENGEAKKVKAKFRVYSSYL
Sbjct: 181 ESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYL 240

Query: 241 EALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAGYATDPHYARKLTNMIQQMKSISDK 300
EALSDYVGLLTRNPRYAAVTTAASAEQGAQ LQDAGYATDPHYARKLTNMIQQMKSISDK
Sbjct: 241 EALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKSISDK 300

Query: 301 VSKTYSMNIDNLF 313
VSKTYSMNIDNLF
Sbjct: 301 VSKTYSMNIDNLF 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06155FLAGELLIN452e-07 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 45.0 bits (106), Expect = 2e-07
Identities = 40/226 (17%), Positives = 79/226 (34%), Gaps = 9/226 (3%)

Query: 7 MMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPIAASQAVVLSQAQAQNSQYTLAR 66
++ Q N+ +S + + E++S+G R+ + DD + A + +Q +
Sbjct: 11 LLTQNNLNKSQSSLSSAI---ERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNA 67

Query: 67 TFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTLSDDDRASLATDIQGLRDQLLNLAN 126
E L+++ +Q +E V A+NGT SD D S+ +IQ +++ ++N
Sbjct: 68 NDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSN 127

Query: 127 TTDGNGRYIFAGYKTETAPFSEANGDYVGGTESIKQQVDASRSMVIGHTGDKIFDSITSN 186
T NG + + +G E+I + +G G + +
Sbjct: 128 QTQFNGVKVLSQDNQMKIQVGANDG------ETITIDLQKIDVKSLGLDGFNVNGPKEAT 181

Query: 187 AVAEPDGSASETNLFAMLDSAIAALKTPVADSEADKEIAAAALDKT 232
+ T A + + A DK
Sbjct: 182 VGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKV 227


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06160IGASERPTASE682e-13 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 68.2 bits (166), Expect = 2e-13
Identities = 49/261 (18%), Positives = 87/261 (33%), Gaps = 26/261 (9%)

Query: 551 VAPAPKAATATPAAPAQPGLLSRFFGALKALFSGGEEAKPTEQP-TPKAEAKPERQQDRR 609
T P + S E A+ E P P A A P
Sbjct: 991 TVDTTNITTPNNIQADVPSVPSN----------NEEIARVDEAPVPPPAPATPSET---- 1036

Query: 610 KPRQSNRRDRNERRDTRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEV------T 663
+ N ++++++ D E +NR A++ + + + Q EV T
Sbjct: 1037 ----TETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSET 1092

Query: 664 EKARTTDEQQAPRRERSRRRNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQR 723
++ +TT+ ++ E+ + + + Q+ K + + QE + + + R
Sbjct: 1093 KETQTTETKETATVEKEEKAKVETEKTQEVPK-VTSQVSPKQEQSETVQPQAEPARENDP 1151

Query: 724 QLNQKVRYEQSVAEEAVVAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEE 783
+N K Q+ P E ++ E V E+ T V P A Q
Sbjct: 1152 TVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTV 1211

Query: 784 NNADNRDNGGMPRRSRRSPRH 804
N+ + RRS RS H
Sbjct: 1212 NSESSNKPKNRHRRSVRSVPH 1232



Score = 61.2 bits (148), Expect = 2e-11
Identities = 48/288 (16%), Positives = 88/288 (30%), Gaps = 36/288 (12%)

Query: 513 PSEEEFAERKRPEQPALATFAMPDVPPAPT-PAEPAATVVAPAPKAATATPAAPAQPGLL 571
P E+ + DVP P+ E A AP P A ATP+ +
Sbjct: 983 PEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTE---- 1038

Query: 572 SRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRDTRSER-- 629
A E +K + K E Q+ + + + ++
Sbjct: 1039 ------TVA-----ENSKQESKTVEKNEQDATE-----TTAQNREVAKEAKSNVKANTQT 1082

Query: 630 TEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRRRNDDKRQ 689
E + + E + + ++TA + + TEK + + + + + + Q
Sbjct: 1083 NEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQ 1142

Query: 690 AQ---QEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSV--AEEAVVAPV 744
A+ + +N++E Q + +P + + Q V +V V P
Sbjct: 1143 AEPARENDPTVNIKEPQSQTNTTADTEQPA--KETSSNVEQPVTESTTVNTGNSVVENPE 1200

Query: 745 VEETAAAEPIVQEAPA------PRTELVKVPLPVVAQTAPEQQEENNA 786
A +P V + R + VP V T A
Sbjct: 1201 NTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVA 1248


79B7485_06650B7485_06710N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_06650-122-3.337871ABC transporter ATP-binding protein
B7485_06655-222-5.587401iron ABC transporter permease
B7485_06660-221-4.221417iron ABC transporter substrate-binding protein
B7485_06665-219-3.320200hypothetical protein
B7485_06670-315-2.551269alpha,alpha-trehalase
B7485_06675-214-2.106570dihydroxyacetone kinase subunit DhaM
B7485_06685-2130.114659dihydroxyacetone kinase subunit L
B7485_06690-2161.306283dihydroxyacetone kinase subunit DhaK
B7485_06700-2120.383859sigma-54-dependent Fis family transcriptional
B7485_06710-1100.905683outer membrane autotransporter barrel
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06770LCRVANTIGEN300.011 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 29.7 bits (66), Expect = 0.011
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 7/63 (11%)

Query: 193 LMSTHHPLHANAIADSIIQVEPDGRVTQGLPTEQLTTNKLAAL------YRVSADQIHHH 246
+ H L A+ I D I++V D G +L +LA L Y V +I+ H
Sbjct: 119 MAVMHFSLTADRIDDDILKVIVDSMNHHGDARSKL-REELAELTAELKIYSVIQAEINKH 177

Query: 247 LSA 249
LS+
Sbjct: 178 LSS 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06780FERRIBNDNGPP401e-05 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 39.5 bits (92), Expect = 1e-05
Identities = 65/301 (21%), Positives = 102/301 (33%), Gaps = 48/301 (15%)

Query: 2 PITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNI--TRIVSAG-APADLLL-L 57
I+RR A+A + L + A I RIV+ P +LLL L
Sbjct: 6 LISRRRLLTAMALSPL-------------LWQMNTAHAAAIDPNRIVALEWLPVELLLAL 52

Query: 58 AVAPEKMVGFSSFDFARQALI--PLPEHIRQLPRLGRLAGRASTLSLEGLMALHPDLVVD 115
+ P G + R + PLP+ + + G + +LE L + P +V
Sbjct: 53 GIVP---YGVADTINYRLWVSEPPLPDSVIDV-------GLRTEPNLELLTEMKPSFMVW 102

Query: 116 CGNTDETLISQARQVSEQTQIPWLLLN-----GKLAQSAEQLTTLGKTLGEEHRAAEQAN 170
+ AR P N LA + + LT + L + A
Sbjct: 103 SAGYGPSPEMLARIA------PGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLA 156

Query: 171 LASHFVGEAQA-FATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLGLHNVAQ-IADR 228
F+ + F A L PR + SL E + G+ N Q +
Sbjct: 157 QYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNF 216

Query: 229 HGLTQVSMENLLRWQ-PDIILVQEAVTADF--IRRDPLWQGVKAVAEQRILFLSGLPFGW 285
G T VS++ L ++ D++ + D + PLWQ + V R +P W
Sbjct: 217 WGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGR---FQRVPAVW 273

Query: 286 L 286

Sbjct: 274 F 274


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06805PHPHTRNFRASE1402e-38 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 140 bits (355), Expect = 2e-38
Identities = 60/206 (29%), Positives = 100/206 (48%), Gaps = 1/206 (0%)

Query: 258 GKAFYYQPVLCTVQAKSTLTAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSG 317
KAF + ++ S E ++L A++ + +L + + EAS D A IF+
Sbjct: 17 AKAFIHLEPNVDIEKTSITDVSTEIEKLTAALEKSKEELRAIKDQTEASMGADKAEIFAA 76

Query: 318 HHTLLGDPELLAAASELLQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLH 377
H +L DPEL+ +++E AEYA ++V ++ +D+EY++ R D+ D+
Sbjct: 77 HLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDNEYMKERAADIRDVSK 136

Query: 378 RTLVHLT-QTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIAR 436
R L HL L T+++AE++ PS QL+ VKG G SHSA+++R
Sbjct: 137 RVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMSR 196

Query: 437 ELGIGWICQQGEKLYAIQPEETLTLD 462
L I + E IQ + + +D
Sbjct: 197 SLEIPAVVGTKEVTEKIQHGDMVIVD 222


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06810adhesinmafb280.020 Neisseria meningitidis: adhesin MafB signature.
		>adhesinmafb#Neisseria meningitidis: adhesin MafB signature.

Length = 467

Score = 28.5 bits (63), Expect = 0.020
Identities = 10/47 (21%), Positives = 26/47 (55%)

Query: 138 VESLRQSSEQNLSVPVALEAASSIAESAAQSTITMQARKGRASYLGE 184
E++ + ++N + +EA ++A +A + + A+ G+A+ G+
Sbjct: 293 REAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGD 339


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06820HTHFIS2447e-76 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 244 bits (625), Expect = 7e-76
Identities = 91/363 (25%), Positives = 155/363 (42%), Gaps = 33/363 (9%)

Query: 308 QMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQA 367
+ S+L S + + + + ++ +++ GE G GK L+++A
Sbjct: 120 AEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARA 179

Query: 368 IHNESERAAGPYIAVNCELYGDAALAEEFIG---GDRTDNENGRLSRLELAHGGTLFLEK 424
+H+ +R GP++A+N + E G G T + R E A GGTLFL++
Sbjct: 180 LHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDE 239

Query: 425 IEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYA 484
I + ++ Q+ LL+V++QG T + R I DV+++A T DL + Q F LYY
Sbjct: 240 IGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYR 299

Query: 485 LHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELY 544
L+ + +PPLR R IP LV + ++ EK + D +AL + + WPGN EL
Sbjct: 300 LNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELE 359

Query: 545 SVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLSTS------------------- 585
+++ L I + L +E + +
Sbjct: 360 NLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASF 419

Query: 586 -----------LSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQ 634
AE+E I+ A T G + + LLG+ R TL +K+++ G+ +
Sbjct: 420 GDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYR 479

Query: 635 FKR 637
R
Sbjct: 480 SSR 482


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06825PRTACTNFAMLY2123e-59 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 212 bits (540), Expect = 3e-59
Identities = 247/980 (25%), Positives = 402/980 (41%), Gaps = 117/980 (11%)

Query: 14 RLAELKIRSPSIQLIKFGAIGLNAIIFSPLLIAADTGSQYGTNITINDGDRI---TGDTA 70
+ A L+ + ++ L GA ++ I Q+G +I +D + +G T
Sbjct: 10 KAAPLRRTTLAMALGALGAAPAAHADWNNQSIVKTGERQHGIHIQGSDPGGVRTASGTTI 69

Query: 71 DPSGN-LYGVMTPAGNTPGNINLGNDVTVN---VNDASGYAKGIIIQGKNSSLTANRLTV 126
SG G++ N + N + ++D + K L A+ T+
Sbjct: 70 KVSGRQAQGILLE--NPAAELQFRNGSVTSSGQLSDDGIRRFLGTVTVKAGKLVADHATL 127

Query: 127 DVVGQT---SAIGINLIGDYTHADLGTGSTIKSNDDGIIIGHSSTLTATQFTIENSNGIG 183
VG T I + + G+ A + ST++ G+ I + +T + I + G+
Sbjct: 128 ANVGDTWDDDGIALYVAGEQAQASIAD-STLQGAG-GVQIERGANVTVQRSAIVD-GGLH 184

Query: 184 LTINDYGTSVDLGSGSKIKTDGS-TGVYIGGLNGNNANGAARFTATDLTID---VQGYSA 239
+ DL + D + T V G + A++LT+D + G A
Sbjct: 185 IGALQSLQPEDLPPSRVVLRDTNVTAVPASGAPA----AVSVLGASELTLDGGHITGGRA 240

Query: 240 MGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSANAL-------TVDVTGAAANGVE 292
G+ + +VV L +TI+ A G G V A+ GV+
Sbjct: 241 AGVAAMQGAVVHL-QRATIRRGDAPAGGAVPGGAVPGGAVPGGFGPGGFGPVLDGWYGVD 299

Query: 293 VRGGTTTIGADSHISSAQGGGLVTSSSDATINFSG---TAAQRNSIFSGGSYGASAQTAT 349
V G + + A S + + + G + A + SG +A N I +GG+ + Q A
Sbjct: 300 VSGSSVEL-AQSIVEAPELGAAIRVGRGARVTVSGGSLSAPHGNVIETGGARRFAPQAAP 358

Query: 350 AVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGARGIYAMTNSQIDLTSDL 409
I +Q G+ A G L L +TG A A+G T + +
Sbjct: 359 LSITLQA--------GAHAQGKALLYRVLPEPVKLTLTGGADAQGDIVATELPSIPGTSI 410

Query: 410 VIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKGGLINLDMHPGSVWTGSSLS 469
P +A+A+ + WTG++
Sbjct: 411 -----GPLDVALAS------------------------------------QARWTGAT-- 427

Query: 470 DNVNGGKLDVAMNNSVWNVTSNSNLDTLAL-SHSTVDFASHGSTAGTFTTLNVENLSGNS 528
V+ +D N+ W +T NSN+ L L S +VDF + AG F L V L+G+
Sbjct: 428 RAVDSLSID----NATWVMTDNSNVGALRLASDGSVDFQQ-PAEAGRFKVLTVNTLAGSG 482

Query: 529 TFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRNQGSEATTGNEVLTVVKTTDGAA 588
F M D L + ++G H L +RN GSE + N +L V AA
Sbjct: 483 LFRMNV------FADLGLSDKLVVMQDASGQHRLWVRNSGSEPASANTLLLVQTPLGSAA 536

Query: 589 SFSASS---QVELGGYLYDVRKNG-TNWELYASGTVPEPTPNPEPTPAPAQPPIVNPD-P 643
+F+ ++ +V++G Y Y + NG W L + P P P P+P P P QPP P+ P
Sbjct: 537 TFTLANKDGKVDIGTYRYRLAANGNGQWSLVGAKAPPAPKPAPQPGPQPPQPPQPQPEAP 596

Query: 644 TPEPAPTPKPTTTADAGGNYLNVGYL--LNYVENRTLMQRMGDLRNQSKDGNIWLRSYG- 700
P+P + + A+A N VG L Y E+ L +R+G+LR G W R +
Sbjct: 597 APQPPAGRELSAAANAAVNTGGVGLASTLWYAESNALSKRLGELRLNPDAGGAWGRGFAQ 656

Query: 701 -GSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVM-PLYVGLYIDSTHASPDYSG-GDGTA 757
LD+ A + FD +G + G D ++ ++G T ++G G G
Sbjct: 657 RQQLDNRAGRR---FDQKVAGFELGADHAVAVAGGRWHLGGLAGYTRGDRGFTGDGGGHT 713

Query: 758 RSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNGVNANGTANGMSISLEAGQR 817
S ++G YA+Y+A +GFY D ++ASR +N F V S V +G+ SLEAG+R
Sbjct: 714 DSVHVGGYATYIADSGFYLDATLRASRLENDFKVAGSDGYAVKGKYRTHGVGASLEAGRR 773

Query: 818 FNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLLGRASMILGYDIT-AG 876
F + G+++EPQ +L A +A+NGL + S+LGR + +G I AG
Sbjct: 774 FTHAD---GWFLEPQAELAVFRAGGGAYRAANGLRVRDEGGSSVLGRLGLEVGKRIELAG 830

Query: 877 NSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGVSAQYNKQHTFYLEADY 936
Q+ Y+K ++EF G N + +G G+G++A + H+ Y +Y
Sbjct: 831 GRQVQPYIKASVLQEFDGAGTVHTNGIAHRTELRGTRAELGLGMAAALGRGHSLYASYEY 890

Query: 937 TQGNLFDQK-QVNGGYRFSF 955
++G + GYR+S+
Sbjct: 891 SKGPKLAMPWTFHAGYRYSW 910


80B7485_06830B7485_06850N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_06830-117-0.917623YchO family inverse autotransporter
B7485_06835-1220.121421DNA-binding response regulator
B7485_06840-1170.959333nitrate/nitrite two-component system sensor
B7485_06845-1141.179017hypothetical protein
B7485_06850-2131.657398NarK family nitrate/nitrite MFS transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06935INTIMIN2554e-78 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 255 bits (652), Expect = 4e-78
Identities = 120/378 (31%), Positives = 197/378 (52%), Gaps = 21/378 (5%)

Query: 79 GEQAKAFALGKVRDALSQQVNQHVESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDN 138
G+ AK ALG + Q + +++WL +G A V+++ N F GS + +P D+
Sbjct: 184 GDYAKDTALGIAGN----QASSQLQAWLQHYGTAEVNLQSGNN--FDGSSLDFLLPFYDS 237

Query: 139 DRYLTWSQLGLTQQDNGLVSNVGVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWG 198
++ L + Q+G D+ +N+G GQR+ ++GYN F D + R G G E W
Sbjct: 238 EKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVFIDQDFSGDNTRLGIGGEYWR 297

Query: 199 EYLRLSANFYQPFAAWHE--QTATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDR 256
+Y + S N Y + WHE ++R A G+D+ +P Y L + EQY+GD
Sbjct: 298 DYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFNGYLPSYPALGAKLMYEQYYGDN 357

Query: 257 VDLFNSGTGYHNPVALSLGLNYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKK 316
V LFNS NP A ++G+NYTP+PLVT+ ++ G EN + Y+F P +
Sbjct: 358 VALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRHGTGNENDLLYSMQFRYQFDKPWSQ 417

Query: 317 QLSAGEVAESQSLRGSRYDNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQI 376
Q+ V E ++L GSRYD QRNN LEY+++ L++ + + T ++L +
Sbjct: 418 QIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQDILSLNI-PHDINGTERSTQKIQLIV 476

Query: 377 RSRYGIRQLIWQGDTQILS-----LTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVV 431
+S+YG+ +++W D+ + S G+Q SA+ + I+P + +G SN ++++
Sbjct: 477 KSKYGLDRIVWD-DSALRSQGGQIQHSGSQ--SAQDYQAILPAYV--QGGSNVYKVTARA 531

Query: 432 EDNQGQRVSSNEITLTLV 449
D G SSN + LT+
Sbjct: 532 YDRNGN--SSNNVLLTIT 547


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06940HTHFIS742e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 73.7 bits (181), Expect = 2e-17
Identities = 32/117 (27%), Positives = 56/117 (47%), Gaps = 2/117 (1%)

Query: 7 ATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQGIELAESLDPDLILLDLNMPGMN 66
ATIL+ DD +RT + Q +S A + SN + D DL++ D+ MP N
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRI--TSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 67 GLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRGADGYLLKDMEPEDLLKALHQA 123
+ L ++++ ++V S N + A ++GA YL K + +L+ + +A
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRA 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06945PF06580531e-09 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 53.3 bits (128), Expect = 1e-09
Identities = 36/172 (20%), Positives = 73/172 (42%), Gaps = 23/172 (13%)

Query: 424 PESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPVKLD 483
P +RE+L+ + + S + +LT +++ + S +F ++ +
Sbjct: 190 PTKAREMLTSLSELMRYSLRYSNARQVSLADELT------VVDSYLQLASIQFEDRLQFE 243

Query: 484 YQLPPRL----VPSHQAIHLLQIAREALSNALKH-----SQASEVVVTVAQNDNQVKLTV 534
Q+ P + VP L+Q E N +KH Q ++++ +++ V L V
Sbjct: 244 NQINPAIMDVQVPPM----LVQTLVE---NGIKHGIAQLPQGGKILLKGTKDNGTVTLEV 296

Query: 535 QDNGCGVPENAIRSNHYGMIIMRDRAQSLRG-DCRVRRRESGGTEVVVTFIP 585
++ G +N S G+ +R+R Q L G + +++ E G + IP
Sbjct: 297 ENTGSLALKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_06955ACRIFLAVINRP330.004 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 32.5 bits (74), Expect = 0.004
Identities = 35/166 (21%), Positives = 60/166 (36%), Gaps = 22/166 (13%)

Query: 258 IMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFIGALARSA---GGALSDR 314
I+S + L+ + I A A L K + + FFG F S ++
Sbjct: 474 IVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKI 533

Query: 315 LGGTRVTLVNFILMAIFSGLLFLTLPTD----GQGGSFMAFFAVFLALFLTAGLGSGSTF 370
LG T L+ + L+ +LFL LP+ G F+ L +G+T
Sbjct: 534 LGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTM----------IQLPAGATQ 583

Query: 371 QMISVIFRKLTMDRVKAEGGSDER-----AMREAATGTAAALGFIS 411
+ + ++T +K E + E + A + F+S
Sbjct: 584 ERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVS 629


81B7485_10525B7485_10565N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_105251212.723327two-component system response regulator
B7485_105302222.405951chemotaxis response regulator protein-glutamate
B7485_105351202.170504chemotaxis protein-glutamate
B7485_105401221.792233methyl-accepting chemotaxis protein
B7485_105450301.547231methyl-accepting chemotaxis protein II
B7485_10555531-3.663511chemotaxis protein CheW
B7485_10560528-3.086989flagellar motor protein MotB
B7485_10565429-0.877041flagellar motor stator protein MotA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10870HTHFIS904e-24 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.5 bits (222), Expect = 4e-24
Identities = 30/105 (28%), Positives = 51/105 (48%), Gaps = 3/105 (2%)

Query: 7 KFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGL 66
LV DD + +R ++ L G++ V + + AG V++D MP+ +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYD-VRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 67 ELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPF 111
+LL I+ LPVL+++A+ I A++ GA Y+ KPF
Sbjct: 64 DLLPRIKKARPD--LPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10875HTHFIS659e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 65.2 bits (159), Expect = 9e-14
Identities = 35/188 (18%), Positives = 72/188 (38%), Gaps = 23/188 (12%)

Query: 1 MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMP 60
M+ +L DD A +R ++ + ++ V + I + D++ DV MP
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 RMDGLDFLEKLMRLRPMPVVMVSSLTGKGS-EVTLRALELGAIDFVTKPQLGIREGMLAY 119
+ D L ++ + RP V+V ++ + + ++A E GA D++ KP + E +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLV--MSAQNTFMTAIKASEKGAYDYLPKP-FDLTELIGII 115

Query: 120 SEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQPLP 179
+AE R +K + + +G S E R + + +
Sbjct: 116 GRALAEPKRRPSKLEDDSQDGMP-----------------LVGRSAAMQEIYRVLARLMQ 158

Query: 180 LSSPALLI 187
++
Sbjct: 159 TDLTLMIT 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10905PF05272310.009 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.8 bits (69), Expect = 0.009
Identities = 22/93 (23%), Positives = 35/93 (37%), Gaps = 11/93 (11%)

Query: 46 LISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPNIEE 105
L +SSP A P + G + ++ PGGGDD GE +++
Sbjct: 384 LADVSSPTAAAGGAGGGEPPKKRDPSAG---AGTDPGGPGGGDD-----GEDPFGEWLDD 435

Query: 106 LKKRM---EQSRLRKLRGDLDQLIESDPKLRAL 135
R+ + L+ R L + + S P L
Sbjct: 436 EVARLRLRGRWLLKPRRAALIEALRSAPALAGC 468


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_10910PF05844330.001 YopD protein
		>PF05844#YopD protein

Length = 295

Score = 33.1 bits (75), Expect = 0.001
Identities = 12/28 (42%), Positives = 22/28 (78%), Gaps = 2/28 (7%)

Query: 76 MDLLALLYRLMAKSRQMGMFSLERDIEN 103
++LL +L+R+ K+R++G+ L+RD EN
Sbjct: 74 VELLLILFRIAQKARELGV--LQRDNEN 99


82B7485_10735B7485_10880N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_10735427-2.049256flagellin FliC
B7485_10740123-1.884795flagellar filament capping protein FliD
B7485_10745-119-1.749322flagellar export chaperone FliS
B7485_10750-316-1.062981flagellar protein FliT
B7485_10755-215-0.698286alpha-amylase
B7485_10760-113-1.199448hypothetical protein
B7485_10765012-0.861082hypothetical protein
B7485_10770-111-0.786484SirA-like protein
B7485_10775012-0.791211phosphoporin PhoE
B7485_10780-210-0.431503hypothetical protein
B7485_10785-313-2.090626multidrug SMR transporter
B7485_10790-115-0.470640integrase
B7485_10795015-0.239969flagellar hook-basal body complex protein FliE
B7485_10800-1130.919686flagellar M-ring protein FliF
B7485_10805-1161.386576flagellar M-ring protein FliF
B7485_10815-1261.877914flagellar motor switch protein FliG
B7485_10820-1271.525726flagellar motor switch protein FliG
B7485_10825-1270.770525flagellar assembly protein FliH
B7485_10835331-3.550388flagellum-specific ATP synthase
B7485_10840226-2.562126flagellar biosynthesis chaperone FliJ
B7485_10845126-3.578833flagellar hook-length control protein FliK
B7485_10850-120-2.035023flagellar basal body-associated protein FliL
B7485_10855-217-0.824006flagellar motor switch protein FliM
B7485_108600170.410732flagellar motor switch protein FliN
B7485_108651141.388357flagellar biosynthetic protein FliO
B7485_108701141.239991flagellar biosynthetic protein FliP
B7485_108750121.470490flagellar biosynthetic protein FliQ
B7485_108800121.384368flagellar biosynthetic protein FliR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11070FLAGELLIN2349e-73 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 234 bits (599), Expect = 9e-73
Identities = 260/551 (47%), Positives = 311/551 (56%), Gaps = 47/551 (8%)

Query: 2 AQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 61
AQVINTNSLSL+TQNN+NK+QS+LSS+IERLSSGLRINSAKDDAAGQAIANRFTSNIKGL
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 TQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTGTNSDSDLDSIQDEIKSRLD 121
TQA+RNANDGIS+AQTTEGAL+EINNNLQR+REL+VQA+ GTNSDSDL SIQDEI+ RL+
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 EIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDLKKIDSDTLGLNGFNVNGGGAV 181
EIDRVS QTQFNGV VL++D MKIQVGANDG+TITIDL+KID +LGL+GFNVNG
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEA 180

Query: 182 A---NTAASKADLVAANATVVGNKYTVSAGYDAAKASDLLAGVSDGDTVQATINNGFGTA 238
++ K V NKY V A V D V A N T
Sbjct: 181 TVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAA-NGQLTTD 239

Query: 239 ASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDTAKGTITIDGSAQDVQISSDGKI 298
+ N D + S T + A GDT +GK+
Sbjct: 240 DAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKV 299

Query: 299 TASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAANNTKATTIDIGGTSISFTGNSTTPD 358
+ T NG +LT A ++ AAN AT S T D
Sbjct: 300 ST--------------TINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFD 345

Query: 359 TITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGYSVNGTTGAVTKGVDSVYVDNNEALTT 418
T + + D A + S V+ + G +
Sbjct: 346 DKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLA---------------- 389

Query: 419 SDTVDFYLQDDGSVTNGSGKAVYKDADGKLTTDAETKAATTADPLKALDEAISSIDKFRS 478
+ DA +TA+PL ++D A+S +D RS
Sbjct: 390 -------------GKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDSALSKVDAVRS 436

Query: 479 SLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKAN 538
SLGA+QNR DSA+TNL NT TNL+ A+SRI+DADYATEVSNMSKAQI+QQAG SVLA+AN
Sbjct: 437 SLGAIQNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQAN 496

Query: 539 QVPQQVLSLLQ 549
QVPQ VLSLL+
Sbjct: 497 QVPQNVLSLLR 507


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11080TYPE3OMBPROT330.003 Type III secretion system outer membrane B protein ...
		>TYPE3OMBPROT#Type III secretion system outer membrane B protein

family signature.
Length = 538

Score = 32.7 bits (74), Expect = 0.003
Identities = 24/72 (33%), Positives = 37/72 (51%), Gaps = 2/72 (2%)

Query: 214 NGMEVSVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQT 273
N E +VAA+N + + A+ + +S AL T++L V+T LT T T ++
Sbjct: 236 NSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTS--LLTPTSLTGGEES 293

Query: 274 AIKDWVNAYNSL 285
+KD VNA L
Sbjct: 294 MLKDQVNALKGL 305


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11110RTXTOXIND300.017 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.2 bits (68), Expect = 0.017
Identities = 10/57 (17%), Positives = 17/57 (29%), Gaps = 2/57 (3%)

Query: 164 RFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRF--RLGMLVFFGMLGWALLTAMNQ 218
R L R + + + A L + P R R M ++L +
Sbjct: 26 RKQLDTPVREKDENEFLPAHLELIETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEI 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11115PF01206936e-29 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 92.5 bits (230), Expect = 6e-29
Identities = 16/71 (22%), Positives = 37/71 (52%)

Query: 7 DYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCPQSINNIPLDARNHGYTVLDIQQ 66
D LD G CP P + + + + GE+L V++ P S+ + ++ G+ +L+ ++
Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64

Query: 67 DGPTIRYLIQK 77
+ T + +++
Sbjct: 65 EDGTYHFRLKR 75


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11135ECOLIPORIN5090.0 E.coli/Salmonella-type porin signature.
		>ECOLIPORIN#E.coli/Salmonella-type porin signature.

Length = 383

Score = 509 bits (1312), Expect = 0.0
Identities = 239/388 (61%), Positives = 282/388 (72%), Gaps = 33/388 (8%)

Query: 1 MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYVR 60
MK+ +A+ V ++L A +A AAEIYNKD NKLDLYGKV+ HYFS + + DGD TY+R
Sbjct: 1 MKRKVLAL--VIPALLAAGAAHAAEIYNKDGNKLDLYGKVDGLHYFSDDSSKDGDQTYMR 58

Query: 61 LGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRNYG 120
+GFKGETQINDQLTG+GQWEY + N E +G++ TRLAFAGLKFGDYGS DYGRNYG
Sbjct: 59 VGFKGETQINDQLTGYGQWEYNVQANTTEGEGANS-WTRLAFAGLKFGDYGSFDYGRNYG 117

Query: 121 VAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQGKND 180
V YD+ WTD+LPEFGGD++T D +MTGR GVATYRN DFFGLVDGLNFA QYQGKN+
Sbjct: 118 VLYDVEGWTDMLPEFGGDSYTYADNYMTGRANGVATYRNTDFFGLVDGLNFALQYQGKNE 177

Query: 181 R----------------TDVTEANGDGFGFSTTYEY-EGFGVGATYAKSDRTNDQVIYGN 223
D+ NGDGFG STTY+ GF GA Y SDRTN+QV G
Sbjct: 178 SQSADDVNIGTNNRNNGDDIRYDNGDGFGISTTYDIGMGFSAGAAYTTSDRTNEQVNAGG 237

Query: 224 NSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFG------NNHIANKAQNFEVV 277
A G A+ W AGLKYDANNIYLAT YSET+NMT +G + +ANK QNFEV
Sbjct: 238 T--IAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPYGKTDKGYDGGVANKTQNFEVT 295

Query: 278 AQYQFDFGLRPSVAYLQSKGKDLG----AWGDQDLIEYIDVGATYYFNKNMSTFVDYKIN 333
AQYQFDFGLRP+V++L SKGKDL D+DL++Y DVGATYYFNKN ST+VDYKIN
Sbjct: 296 AQYQFDFGLRPAVSFLMSKGKDLTYNNVNGDDKDLVKYADVGATYYFNKNFSTYVDYKIN 355

Query: 334 LIDKSD-FTKASGVATDDIVAVGLVYQF 360
L+D D F K +G++TDDIVA+G+VYQF
Sbjct: 356 LLDDDDPFYKDAGISTDDIVALGMVYQF 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11170FLGHOOKFLIE1148e-37 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 114 bits (286), Expect = 8e-37
Identities = 101/103 (98%), Positives = 101/103 (98%)

Query: 2 SAIQGIEGVISQLQATAMSARAQESLSQPTISFAGQLHAALDRISDTQTVARTQAEKFTL 61
SAIQGIEGVISQLQATAMSARAQESL QPTISFAGQLHAALDRISDTQT ARTQAEKFTL
Sbjct: 1 SAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTL 60

Query: 62 GEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 104
GEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV
Sbjct: 61 GEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11175FLGMRINGFLIF375e-131 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 375 bits (965), Expect = e-131
Identities = 231/259 (89%), Positives = 246/259 (94%)

Query: 3 ATAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGA 62
+TA Q K LEWLNRLRANP+IPLIV GSAAVA++VA++LWAK PDYRTLFSNLSDQDGGA
Sbjct: 5 STATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGA 64

Query: 63 IVSQLTQMNIPYRFSEASGAIEVPADKVHELHLRLAQQGLPKGGAVGFELLDQEKFGISQ 122
IV+QLTQMNIPYRF+ SGAIEVPADKVHEL LRLAQQGLPKGGAVGFELLDQEKFGISQ
Sbjct: 65 IVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQ 124

Query: 123 FSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPSLFVREQKSPSASVTVNLLPGRA 182
FSEQVNYQRALEGEL+RTIET+GPVK ARVHLAMPKPSLFVREQKSPSASVTV L PGRA
Sbjct: 125 FSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRA 184

Query: 183 LDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSGRDLNDAQLKYASDVEGRI 242
LDEGQISA+VHLVSSAVAGLPPGNVTLVDQ GHLLTQSNTSGRDLNDAQLK+A+DVE RI
Sbjct: 185 LDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDVESRI 244

Query: 243 QQRIEAILSPIVGNGNIHA 261
Q+RIEAILSPIVGNGN+HA
Sbjct: 245 QRRIEAILSPIVGNGNVHA 263


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11180FLGMRINGFLIF2461e-80 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 246 bits (629), Expect = 1e-80
Identities = 159/199 (79%), Positives = 174/199 (87%), Gaps = 5/199 (2%)

Query: 1 MDFASKEQTEEQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPIS 60
+DFA+KEQTEE Y PNGD S A LRSRQLN SEQ G+GYPGGVPGALSNQPAP N API+
Sbjct: 269 LDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGALSNQPAPPNEAPIA 328

Query: 61 TPPTNQNNRQQ--QASTTSNS---GPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAV 115
TPPTNQ N Q Q ST++NS GPRSTQRNETSNYEVDRTIRHTKMNVGD++RLSVAV
Sbjct: 329 TPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHTKMNVGDIERLSVAV 388

Query: 116 VVNYKTLPDGKPLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPF 175
VVNYKTL DGKPLPL+ +QMKQIE LTREAMGFS+KRGD+LNVVNSPF++ D +GG LPF
Sbjct: 389 VVNYKTLADGKPLPLTADQMKQIEDLTREAMGFSDKRGDTLNVVNSPFSAVDNTGGELPF 448

Query: 176 WQQQVFIDQLLAAGRWLLV 194
WQQQ FIDQLLAAGRWLLV
Sbjct: 449 WQQQSFIDQLLAAGRWLLV 467


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11185FLGMOTORFLIG1371e-42 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 137 bits (347), Expect = 1e-42
Identities = 45/140 (32%), Positives = 82/140 (58%), Gaps = 1/140 (0%)

Query: 1 MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANVTQISNKQLTDVLAEFE 60
+S LTG K+ ILL++IG + +++VFK+LSQ E+++L+ +A + I+++ +VL EF+
Sbjct: 12 VSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEFK 71

Query: 61 QEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRDTASGIETLNFMEPQSAAD 120
+ + DY R +L K+LG ++A ++ + L + + E + +P + +
Sbjct: 72 ELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINN-LGSALQSRPFEFVRRADPANILN 130

Query: 121 LIRDEHPQIIATILVHLKRA 140
I+ EHPQ IA IL +L
Sbjct: 131 FIQQEHPQTIALILSYLDPQ 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11190FLGMOTORFLIG2002e-66 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 200 bits (511), Expect = 2e-66
Identities = 69/182 (37%), Positives = 109/182 (59%), Gaps = 1/182 (0%)

Query: 1 MFDERLRHDVMLRIATFGGVQPAALAELTEVLNGLLDGQ-NLKRSKMGGVRTAAEIINLM 59
++ +V RIA P + E+ VL L + + GGV EIIN+
Sbjct: 158 SLPTEVQTNVARRIALMDRTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMA 217

Query: 60 KTQQEEAVITAVREFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGA 119
+ E+ +I ++ E D ELA++I +MF+FE++V +DDRSIQR+L+E+D + L ALK
Sbjct: 218 DRKTEKFIIESLEEEDPELAEEIKKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSV 277

Query: 120 EQPLREKFLRNMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSG 179
+ P++EK +NMS+RAA +L++D+ GP R VE Q+ I+ ++R+L E GE+VI G
Sbjct: 278 DIPVQEKIFKNMSKRAASMLKEDMEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRG 337

Query: 180 ED 181
+
Sbjct: 338 GE 339


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11195FLGFLIH373e-135 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 373 bits (958), Expect = e-135
Identities = 223/228 (97%), Positives = 226/228 (99%)

Query: 1 MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQLAQLQMQAHEQGYQAGI 60
MSDNLPWKTWTPDDLAPP AEFVP+VE EETIIEEAEPSLEQQLAQLQMQAHEQGYQAGI
Sbjct: 1 MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQAHEQGYQAGI 60

Query: 61 AEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLVSEFQTTLDALDSVIASRL 120
AEGRQQGH+QGYQEGLAQGLEQGLAEAK+QQAPIHARMQQLVSEFQTTLDALDSVIASRL
Sbjct: 61 AEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIASRL 120

Query: 121 MQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT 180
MQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT
Sbjct: 121 MQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLGAT 180

Query: 181 LSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELCRLAAPGVV 228
LSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELCRLAAPGVV
Sbjct: 181 LSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELCRLAAPGVV 228


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11205FLGFLIJ1525e-51 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 152 bits (384), Expect = 5e-51
Identities = 112/113 (99%), Positives = 113/113 (100%)

Query: 2 AEEQLKMLIDYQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKV 61
AEEQLKMLIDYQNEYRNNLNSDMSAG+TSNRWINYQQFIQTLEKAITQHRQQLNQWTQKV
Sbjct: 35 AEEQLKMLIDYQNEYRNNLNSDMSAGITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKV 94

Query: 62 DIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE 114
DIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE
Sbjct: 95 DIALNSWREKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE 147


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11210FLGHOOKFLIK468e-168 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 468 bits (1205), Expect = e-168
Identities = 363/375 (96%), Positives = 368/375 (98%)

Query: 1 MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTDKAAPQLLVATDKPTTK 60
MIRLAPLITADVDTTTLPGGKASDAAQDFL LLSEALAGETTTDKAAPQLLVATDKPTTK
Sbjct: 1 MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTDKAAPQLLVATDKPTTK 60

Query: 61 GEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQTMTLAAVADKNTTKDEKA 120
GEPL+SDI++DAQQA+LLIPVDET PVINDEQSTSTPLTTAQTM LAAVADKNTTKDEKA
Sbjct: 61 GEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQTMALAAVADKNTTKDEKA 120

Query: 121 DDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKPTLFTKLTSAQLTTAQPDDAP 180
DDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLP EKPTLFTKLTS QLTTAQPDDAP
Sbjct: 121 DDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKPTLFTKLTSEQLTTAQPDDAP 180

Query: 181 GTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPHQTQPLPTVAAPVLSVPLGSHEW 240
GTPAQPLTPLVAEAQSKAEVISTPSPVTA ASPLITPHQTQPLPTVAAPVLS PLGSHEW
Sbjct: 181 GTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPHQTQPLPTVAAPVLSAPLGSHEW 240

Query: 241 QQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMISPHQHVRAALEAA 300
QQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQM+SPHQHVRAALEAA
Sbjct: 241 QQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAA 300

Query: 301 LPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVS 360
LPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVS
Sbjct: 301 LPVLRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQRTANHEPLAGEDDDTLPVPVS 360

Query: 361 LQGRVTGNSGVDIFA 375
LQGRVTGNSGVDIFA
Sbjct: 361 LQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11220FLGMOTORFLIM379e-134 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 379 bits (975), Expect = e-134
Identities = 85/324 (26%), Positives = 148/324 (45%), Gaps = 10/324 (3%)

Query: 5 ILSQAEIDALLNGDS--EVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFA 62
+LSQ EID LL S + E +S I YD + +E+++ L +++E FA
Sbjct: 4 VLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFA 63

Query: 63 RHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSL 122
R L LR + V ++ Y EF R++P P+ L +I + PL+G ++ PS+
Sbjct: 64 RLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPSI 123

Query: 123 VFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYVRS 182
F +D LFGG G+ KV+ R+ T E V+ ++ L ++W + L +
Sbjct: 124 TFSIIDRLFGGTGQ-AAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQI 181

Query: 183 EMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENS--R 240
E +F I P+++VV ++G G N C+P+ IEP+ L + +S R
Sbjct: 182 ETNPQFAQI-VPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRR 240

Query: 241 NEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKP---DRIIAHVD 297
+ + L ++ +++VA + L + IL L+ GD++ + D + +
Sbjct: 241 SSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIG 300

Query: 298 GVPVLTSQYGTLNGQYALRIEHLI 321
Q G + + A +I I
Sbjct: 301 NRKKFLCQPGVVGKKIAAQILERI 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11225FLGMOTORFLIN2106e-74 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 210 bits (537), Expect = 6e-74
Identities = 125/137 (91%), Positives = 133/137 (97%)

Query: 1 MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGGGDVSGTLQDIDLIMDI 60
MSDMNNP+D+N GA+DDLWA+AL+EQK+T+ KSAADAVFQQ GGGDVSG +QDIDLIMDI
Sbjct: 1 MSDMNNPSDENTGALDDLWADALNEQKATTTKSAADAVFQQLGGGDVSGAMQDIDLIMDI 60

Query: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120
PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV
Sbjct: 61 PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV 120

Query: 121 RITDIITPSERMRRLSR 137
RITDIITPSERMRRLSR
Sbjct: 121 RITDIITPSERMRRLSR 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11235FLGBIOSNFLIP333e-119 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 333 bits (855), Expect = e-119
Identities = 243/245 (99%), Positives = 244/245 (99%)

Query: 1 MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLM 60
MRRL SVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLM
Sbjct: 1 MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLM 60

Query: 61 MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVINKIYVDAYQPFSEEK 120
MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVI+KIYVDAYQPFSEEK
Sbjct: 61 MTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEK 120

Query: 121 ISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELK 180
ISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELK
Sbjct: 121 ISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELK 180

Query: 181 TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA 240
TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA
Sbjct: 181 TAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLA 240

Query: 241 QSFYS 245
QSFYS
Sbjct: 241 QSFYS 245


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11240TYPE3IMQPROT671e-18 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 67.1 bits (164), Expect = 1e-18
Identities = 22/78 (28%), Positives = 42/78 (53%)

Query: 4 ESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQINEMTLSFIPKIIAVFIAII 63
+ ++ G +A+ + L L+ +VA + GL++ + Q TQ+ E TL F K++ V + +
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 IAGPWMLNLLLDYVRTLF 81
+ W +LL Y R +
Sbjct: 62 LLSGWYGEVLLSYGRQVI 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_11245TYPE3IMRPROT2034e-67 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 203 bits (517), Expect = 4e-67
Identities = 254/261 (97%), Positives = 257/261 (98%)

Query: 1 MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60
M+Q TS+QWLSWL+LYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPGSHL 120
NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDP SHL
Sbjct: 61 NDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHL 120

Query: 121 NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIF 180
NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG EPLNSNAFLA TKAGSLIF
Sbjct: 121 NMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIF 180

Query: 181 LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC 240
LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC
Sbjct: 181 LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFC 240

Query: 241 EHLFSEIFNLLADIISELPLI 261
EHLFSEIFNLLADIISELPLI
Sbjct: 241 EHLFSEIFNLLADIISELPLI 261


83B7485_11740B7485_11810N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_11740022-3.310870molecular chaperone
B7485_11745122-3.064378molecular chaperone
B7485_11765119-0.8533683-methyladenine DNA glycosylase 2
B7485_11775227-2.580486IS110 family transposase
B7485_11785529-0.184975multidrug transporter subunit MdtA
B7485_117904220.687578multidrug transporter subunit MdtC
B7485_117953220.734871multidrug transporter subunit MdtD
B7485_118053230.976314two-component sensor histidine kinase
B7485_118103270.349129DNA-binding response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12175SHAPEPROTEIN362e-05 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 35.5 bits (82), Expect = 2e-05
Identities = 30/127 (23%), Positives = 52/127 (40%), Gaps = 20/127 (15%)

Query: 18 RSAEECKIALSSV--AETRASLPFISNELAT------LISQRGLESALSQPLARILEQVQ 69
+AE K + S + + LA ++ + AL +PL I+ V
Sbjct: 213 ATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVM 272

Query: 70 LALDNAQEKPDV--------IYLTGGSARSPLIKKALAEQLPGIPIAGGDD-FGSVTAGL 120
+AL+ Q P++ + LTGG A + + L E+ GIP+ +D V G
Sbjct: 273 VALE--QCPPELASDISERGMVLTGGGALLRNLDRLLMEET-GIPVVVAEDPLTCVARGG 329

Query: 121 ARWAEVV 127
+ E++
Sbjct: 330 GKALEMI 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12180SHAPEPROTEIN503e-09 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 50.1 bits (120), Expect = 3e-09
Identities = 32/129 (24%), Positives = 57/129 (44%), Gaps = 20/129 (15%)

Query: 132 AMMLH-IRQQAQAQLPEAITQAVIGRPINFQGLGGDEANAQAQGILERAAKRAGFKDVVF 190
M+ H I+Q + ++ P+ + E A + +A+ AG ++V
Sbjct: 89 KMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQV---ERRA-----IRESAQGAGAREVFL 140

Query: 191 QYEPVAAGLDYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLLGHSGYRI 250
EP+AA + + E +VVDIGGGTT+ +++ + ++ S RI
Sbjct: 141 IEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN-----------GVVYSSSVRI 189

Query: 251 GGNDLDIAL 259
GG+ D A+
Sbjct: 190 GGDRFDEAI 198


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12200RTXTOXIND445e-07 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 44.0 bits (104), Expect = 5e-07
Identities = 33/167 (19%), Positives = 64/167 (38%), Gaps = 11/167 (6%)

Query: 61 ALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIKADEAS 120
+ +L K+ L ++ AK +L + L + T +
Sbjct: 260 KYVEAVNELRVYKSQLEQIESEILS----AKEEYQLVTQLFKNEILDKLRQTTDNIGLLT 315

Query: 121 --VASAQLQLDWSRITAPVDGRV-GLKQVDVGNQISSGDTTGIVVITQTHPIDLVFTLPE 177
+A + + S I APV +V LK G +++ +T +V++ + +++ +
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETL-MVIVPEDDTLEVTALVQN 374

Query: 178 SDIATVVQAQKAGKPLMVEAWDRTNSKKL-SEGTLLSLDNQIDATTG 223
DI + Q A + VEA+ T L + ++LD D G
Sbjct: 375 KDIGFINVGQNA--IIKVEAFPYTRYGYLVGKVKNINLDAIEDQRLG 419



Score = 43.7 bits (103), Expect = 8e-07
Identities = 21/122 (17%), Positives = 47/122 (38%), Gaps = 13/122 (10%)

Query: 15 GTITAA-NTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDK 73
G +T + + ++ + + + +EG+ V+ GD+L ++ + K +
Sbjct: 88 GKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTAL-------GAEADTLKTQ 140

Query: 74 ATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRI 133
++L AR + RYQ L+++ EL+ L E + L +
Sbjct: 141 SSLLQARLEQTRYQILSRS-----IELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQF 195

Query: 134 TA 135
+
Sbjct: 196 ST 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12210ACRIFLAVINRP9070.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 907 bits (2345), Expect = 0.0
Identities = 287/1035 (27%), Positives = 502/1035 (48%), Gaps = 40/1035 (3%)

Query: 6 LFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPVIMVSASLPGASPETMASSVAT 65
FI RP+ +L++ + + G L LPVA P + P + VSA+ PGA +T+ +V
Sbjct: 4 FFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQ 63

Query: 66 PLERSLGRIAGVSEMTSSS-SLGSTRIILQFDFDRDINGAARDVQAAINAAQSLLPSGMP 124
+E+++ I + M+S+S S GS I L F D + A VQ + A LLP +
Sbjct: 64 VIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQ 123

Query: 125 SRPTYRKANPSDAPIMILTLTSDT--YSQGELYDFASTQLAPTISQIDGVGDVDVGGSSL 182
+ S + +M+ SD +Q ++ D+ ++ + T+S+++GVGDV + G+
Sbjct: 124 -QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY 182

Query: 183 PAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG------ALEDGTHRWQIQTNDELK 236
A+R+ L L ++ DV + N + G AL I K
Sbjct: 183 -AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 237 TAAEYQPLIIHYN-NGGAVRLGDVATVTDSVQDVRNAGMTNAKPAILLMIRKLPEANIIQ 295
E+ + + N +G VRL DVA V ++ N KPA L I+ AN +
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALD 301

Query: 296 TVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEVEQTLIISVALVILVVFLFLRS 355
T +I+AKL ELQ P + + D +P ++ S+ EV +TL ++ LV LV++LFL++
Sbjct: 302 TAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQN 361

Query: 356 GRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMALTIATGFVVDDAIVVLENIARHL- 414
RAT+IP +AVPV L+GTFA + G+S+N L++ + +A G +VDDAIVV+EN+ R +
Sbjct: 362 MRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM 421

Query: 415 EAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLLLMGGLPGRLLREFAVTLSVAIGIS 474
E + P +A + ++ ++ +++ L AVF+P+ GG G + R+F++T+ A+ +S
Sbjct: 422 EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALS 481

Query: 475 LLVSLTLTPMMCGWMLKASKPREQKRLRGFG----RMLVALQQGYGKSLKWVLNHTRLVG 530
+LV+L LTP +C +LK + GF Y S+ +L T
Sbjct: 482 VLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYL 541

Query: 531 VVLLGTIALNI----SIPKTFFPEQDTGVLMGGIQADQSISFQ----AMRGKLQDFMKII 582
++ +A + +P +F PE+D GV + IQ + + + ++K
Sbjct: 542 LIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNE 601

Query: 583 RD-DPAVDNVTGFT-GGSRVNSGMMFITLKPRDERS---ETAQQIIDRLRVKLAKEPGAN 637
+ +V V GF+ G N+GM F++LKP +ER+ +A+ +I R +++L K
Sbjct: 602 KANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 638 LFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLATL-----PELADVNSD 692
+ + I G + ++ L D + + R +L + L V +
Sbjct: 662 VIPFNMPAIVELGTATGFDFE---LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 693 QQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRY 752
++ A+ L D++ LG+ + N ++ A G ++ K+ ++ D ++
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 753 TQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAASTISFNLPTGKSLSD 812
++K++V + G+ +P S F + + I G S D
Sbjct: 779 RMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGD 838

Query: 813 ASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAIATVYIVLGILYESYVH 872
A A ++ ++L P+ + + G + + + N L+ + V++ L LYES+
Sbjct: 839 AMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSI 896

Query: 873 PLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRHGN 932
P++++ +P VG LLA LFN + ++G++ IG+ KNAI++V+FA +
Sbjct: 897 PVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEG 956

Query: 933 LTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLEITIVGGLVMSQLL 992
EA A +R RPI+MT+LA + G LPL +S G GS + + I ++GG+V + LL
Sbjct: 957 KGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLL 1016

Query: 993 TLYTTPVVYLFFDRL 1007
++ PV ++ R
Sbjct: 1017 AIFFVPVFFVVIRRC 1031



Score = 78.3 bits (193), Expect = 1e-16
Identities = 77/446 (17%), Positives = 162/446 (36%), Gaps = 26/446 (5%)

Query: 588 VDNVTGFTGGS-RVNSGMMFITLKPRDERSETAQQIIDRLRVKLAKEPGANLFLMAVQDI 646
+DN+ + S S + +T + + Q+ ++L++ P + Q I
Sbjct: 72 IDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE----VQQQGI 127

Query: 647 RVGGRQSNASYQYTLLSDDLAALREW-----EPKIRKKLATLPELADVNSDQQDNGAE-- 699
V S+ +SD+ ++ ++ L+ L + DV GA+
Sbjct: 128 SVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL----FGAQYA 183

Query: 700 MNLVYDRDTMARLGID----VQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYTQD 755
M + D D + + + + + + T P Q + R+
Sbjct: 184 MRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKNP 243

Query: 756 ISALEKMFVINNEGKAIPLSYFAK--WQPANAPLSVNHQGLSAASTISFNLPTGKSLSDA 813
+ +N++G + L A+ N + G AA +L D
Sbjct: 244 EEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL-DT 302

Query: 814 SAAIDRAMTQL--GVPSTVRGSFA-GTAQVFQETMNSQVILIIAAIATVYIVLGILYESY 870
+ AI + +L P ++ + T Q +++ V + AI V++V+ + ++
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 871 VHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRH 930
L +P +G L F + + + G++L IG++ +AI++V+
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 931 GNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLEITIVGGLVMSQ 990
L P+EA ++ ++ + +P+ GG + + ITIV + +S
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 991 LLTLYTTPVVYLFFDRLRLRFSRKPK 1016
L+ L TP + + + K
Sbjct: 483 LVALILTPALCATLLKPVSAEHHENK 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12215TCRTETB1251e-33 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 125 bits (315), Expect = 1e-33
Identities = 97/429 (22%), Positives = 187/429 (43%), Gaps = 23/429 (5%)

Query: 20 FMQSLDTTIVNTALPSMAQSLGESPLHMLMVIVSYVLTVAVMLPASGWLADKVGVRNIFF 79
F L+ ++N +LP +A + P V +++LT ++ G L+D++G++ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 80 TAIVLFTLGSLFCALSGTLNELL-LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTF 138
I++ GS+ + + LL +AR +QG G A + + V + +P+E A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 139 VTLPGQVGPLLGPALGGLLVEYASWHWIFLINIPVGIIGAIATLM-LMPNYTMQTRRFDL 197
+ +G +GPA+GG++ Y HW +L+ IP+ I + LM L+ FD+
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHY--IHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 198 SGFLLLAVGMAVLTLALDGSKGTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKL 257
G +L++VG+ L + + V V++ ++++ H R L
Sbjct: 202 KGIILMSVGIVFFMLF---------TTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGL 252

Query: 258 FRTRTFSLGLAGSFAGRIGSGMLPFMTPVFLQIGLGFSPFHAG-LMMIPMVLGSMGMKRI 316
+ F +G+ M P ++ S G +++ P + + I
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312

Query: 317 VVQVVNRFGYRRVLVATTLGLSLVTLLFMTTALL----GWYYVLPFVLFLQGMVNSTRFS 372
+V+R G VL +G++ +++ F+T + L W+ + V L G+ S +
Sbjct: 313 GGILVDRRGPLYVL---NIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL--SFTKT 367

Query: 373 SMNTLTLKDLPDNLASSGNSLLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVF 432
++T+ L A +G SLL+ LS G+ I G LL + + Q+ +
Sbjct: 368 VISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTY 427

Query: 433 MYTWLSMAF 441
+Y+ L + F
Sbjct: 428 LYSNLLLLF 436


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12220BCTERIALGSPF310.009 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 31.3 bits (71), Expect = 0.009
Identities = 27/93 (29%), Positives = 34/93 (36%), Gaps = 27/93 (29%)

Query: 173 LATLLAALATFLLA-------------RGLLAPVKRLVDGTHKLAAGDFTTRVTPTSEDE 219
LATL+AA A L+A V+ V H LA + P S +
Sbjct: 77 LATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLAD---AMKCFPGSFER 133

Query: 220 L-----------GKLAQDFNQLASTLEKNQQMR 241
L G L N+LA E+ QQMR
Sbjct: 134 LYCAMVAAGETSGHLDAVLNRLADYTEQRQQMR 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12225HTHFIS766e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 75.6 bits (186), Expect = 6e-18
Identities = 28/136 (20%), Positives = 65/136 (47%), Gaps = 1/136 (0%)

Query: 11 PRILIVEDEPKLGQLLIDYLRAASYAPTLISHGDQVLAYVRQTPPDLILLDLMLPGTDGL 70
IL+ +D+ + +L L A Y + S+ + ++ DL++ D+++P +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 71 TLCREIR-RFSDVPIVMVTAKIEEIDRLLGLEIGADDYICKPYSPREVVARVKTILRRCK 129
L I+ D+P+++++A+ + + E GA DY+ KP+ E++ + L K
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123

Query: 130 PQRELQQQDAESPLII 145
+ + D++ + +
Sbjct: 124 RRPSKLEDDSQDGMPL 139


84B7485_12050B7485_12085N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_12050-1212.636900hypothetical protein
B7485_12055-1191.924164hypothetical protein
B7485_12060-1181.399265two-component system response regulator YehT
B7485_12065-115-0.577776sensor histidine kinase
B7485_12070-214-0.782265IS4 family transposase
B7485_12075016-1.205106damage-inducible protein DinI
B7485_12080-115-0.701783tail fiber assembly protein
B7485_12085-3180.135072class I SAM-dependent methyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12460INTIMIN280.015 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 28.1 bits (62), Expect = 0.015
Identities = 20/92 (21%), Positives = 31/92 (33%)

Query: 38 GTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTY 97
+ AITY K K K S ++ F + KT AK + KS
Sbjct: 673 NGQDAITYTVKVMKGDKPVSNQEVTFTTTLGKLSNSTEKTDTNGYAKVTLTSTTPGKSLV 732

Query: 98 TDTYAQENVTIDMEKVDFKALQGISGINVSAE 129
+ + V + +V+F I N+
Sbjct: 733 SARVSDVAVDVKAPEVEFFTTLTIDDGNIEIV 764


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12470HTHFIS721e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 71.8 bits (176), Expect = 1e-16
Identities = 41/178 (23%), Positives = 76/178 (42%), Gaps = 14/178 (7%)

Query: 2 IKVLIVDDEPLARENL-RIFLQEQSDIEIVGECSNAVEGIGAVHKLRPDVLFLDIQMPRI 60
+L+ DD+ R L + + D+ I NA + D++ D+ MP
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITS---NAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 SGLEMVGMLDPEHRPYI--VFLTAFD--EYAIKAFEEHAFDYLLKPIDEARLEKTLARLR 116
+ +++ + + RP + + ++A + AIKA E+ A+DYL KP D L + R
Sbjct: 61 NAFDLLPRIK-KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 117 QERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRMSGVYVT--SHEGKE 172
E ++ L ++Q + + G S + +A + + +T S GKE
Sbjct: 120 AEPKRRPSKLEDDSQDGMPLV---GRSAAMQEIYRVLARLMQTDLTLMITGESGTGKE 174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12475PF065802204e-69 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 220 bits (562), Expect = 4e-69
Identities = 63/216 (29%), Positives = 115/216 (53%), Gaps = 3/216 (1%)

Query: 343 LGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQA 402
L G + + + +M ++++ L AQ+NPHF+FNALN I+A+I D +A
Sbjct: 134 LYFGWHFFKNYKQAEIDQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKA 193

Query: 403 SQLVQYLSTFFRKNLKR-PSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQ 461
+++ LS R +L+ + V+LADE+ V++YLQ+ +F+ RLQ I +
Sbjct: 194 REMLTSLSELMRYSLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDV 253

Query: 462 QLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGL-YQPVTNASGL 520
Q+P +Q +VEN IKHG +QL G++ + ++ + LE+E+ L + ++G
Sbjct: 254 QVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGT 313

Query: 521 GMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLP 556
G+ V +RL+ +G + I ++ + + +P
Sbjct: 314 GLQNVRERLQMLYGTEAQIKLSEKQGKVN-AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_12495LUXSPROTEIN310.001 Bacterial autoinducer-2 (AI-2) production protein Lu...
		>LUXSPROTEIN#Bacterial autoinducer-2 (AI-2) production protein LuxS

signature.
Length = 171

Score = 31.4 bits (71), Expect = 0.001
Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 7/66 (10%)

Query: 27 TKEHLLPHFL-EHLGNNHLDI------GVGTGFYLTHVPESSLISLMDLNEASLNAASTR 79
T EHL F+ HL + ++I G TGFY++ + S + D A++
Sbjct: 54 TLEHLYAGFMRNHLNGDSVEIIDISPMGCRTGFYMSLIGTPSEQQVADAWIAAMEDVLKV 113

Query: 80 AGESKI 85
++KI
Sbjct: 114 ENQNKI 119


85B7485_13635B7485_13650N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_13635-114-1.125797MFS transporter
B7485_13640-112-2.134461EmrA/EmrK family multidrug efflux transporter
B7485_13645-112-0.710573DNA-binding response regulator
B7485_13650-116-0.257937two-component system sensor histidine kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13910TCRTETB1193e-31 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 119 bits (300), Expect = 3e-31
Identities = 98/408 (24%), Positives = 168/408 (41%), Gaps = 25/408 (6%)

Query: 19 VTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQ 78
+ I L + +F +L+ + NV++P I+ WV T+F + +I V G+L+
Sbjct: 15 ILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD 74

Query: 79 RIGELRLFLLSVTFFSLSSLMCSLS-TNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPP 137
++G RL L + S++ + + +LI R +QG A L ++ R P
Sbjct: 75 QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPK 134

Query: 138 EKRTFALALWSMTVIIAPICGPILGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRE 197
E R A L V + GP +GG I W +L+ +PM I+ L L +E
Sbjct: 135 ENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKE 192

Query: 198 TETSPVKMNLPRLTLLVLGVGGLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWES 257
K + ++++ VG + ML F +S I +VSV+S + V
Sbjct: 193 VRI---KGHFDIKGIILMSVGIVFFML--------FTTSYSISFLIVSVLSFLIFVKHIR 241

Query: 258 TSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPI 317
+P +D L K+ F IG++ + +G + ++P +++ + G
Sbjct: 242 KVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFP 301

Query: 318 GIMPLLISPLIG-----RYGNKIDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQ 372
G M ++I IG R G + + VTF +V + S T F II+
Sbjct: 302 GTMSVIIFGYIGGILVDRRGPLYVLNIGVTFL----SVSFLTASFLLETTSWFMTIIIVF 357

Query: 373 FFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFFRTLSGSVGTSL 420
G + ++TI S L + S+ NF LS G ++
Sbjct: 358 VLGGLSFTK--TVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAI 403


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13915RTXTOXIND771e-17 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 77.2 bits (190), Expect = 1e-17
Identities = 63/419 (15%), Positives = 125/419 (29%), Gaps = 96/419 (22%)

Query: 8 KKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMISTDDAYVT-GNADPISAQVSGSVT 66
+ +R+ I+ F+ + + ++E + + + G + I + V
Sbjct: 50 ETPVSRRPRLVAYFIMGFLVIAFILSVLG-QVEIVATANGKLTHSGRSKEIKPIENSIVK 108

Query: 67 VVNHKDTNYVRQGDILVSLDKTDATIALNKA----------------------------- 97
+ K+ VR+GD+L+ L A K
Sbjct: 109 EIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPEL 168

Query: 98 -----------------------KNNLANIVRQTNKLYLQDKQYSAEVASARIQ---YQQ 131
K + Q + L + AE + + Y+
Sbjct: 169 KLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYEN 228

Query: 132 SLEDYNRRV----PLAKQGVISKE----------TLEHTKDTLISSKAALNAAIQAYKAN 177
R+ L + I+K + S + + I + K
Sbjct: 229 LSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEE 288

Query: 178 KALVMN-------TPLNR-QPQVVEAADATKEAWLVLKRTDIRSPVTGYIAQRSVQ-VGE 228
LV L + + + + + IR+PV+ + Q V G
Sbjct: 289 YQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGG 348

Query: 229 TVSSGQSLMAVVPARQ-MWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINM 287
V++ ++LM +VP + V A + + + +GQ+ I + F G +
Sbjct: 349 VVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVE------AFPYTRYGYLV 402

Query: 288 GTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEH----PLRIGLSMTATIDTKD 342
G + + +V V +S++ L PL G+++TA I T
Sbjct: 403 GK---VKNINLDAIEDQRLGLVFNVI--ISIEENCLSTGNKNIPLSSGMAVTAEIKTGM 456


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13920HTHFIS493e-09 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 49.1 bits (117), Expect = 3e-09
Identities = 22/148 (14%), Positives = 53/148 (35%), Gaps = 31/148 (20%)

Query: 4 IIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKPDIVIIDVDIPGVNGIQV 63
++ DD + L + ++ + + + + D+V+ DV +P N +
Sbjct: 7 LVADDDAAIRTVLNQALSRAGYDVRIT-SNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 64 LETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKEGMNNIIAAIEAAKNGYCYF 123
L ++K + ++++SA+N + AI+A++ G +
Sbjct: 66 LPRIKKARPDLPVLVMSAQNT------------------------FMTAIKASEKGAYDY 101

Query: 124 ---PFSLNRFVGSLTSDQQKLDSLSKQE 148
PF L + + L ++
Sbjct: 102 LPKPFDLTE---LIGIIGRALAEPKRRP 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_13925HTHFIS802e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 79.9 bits (197), Expect = 2e-17
Identities = 30/105 (28%), Positives = 51/105 (48%)

Query: 960 SILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFE 1019
+IL+ADD R +L + L+ GYDV ++ ++ DL++TDV MP+ + F+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 1020 LTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLD 1064
L ++++ LP+ ++A K G L KP L
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109


86B7485_16570B7485_16605N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_16570-1211.555470nucleoside permease NupG
B7485_165753182.159824ornithine decarboxylase
B7485_165800193.382024hypothetical protein
B7485_16590-1153.010794transporter
B7485_16595-1161.636691*hypothetical protein
B7485_16600-1162.040255IS3 family transposase
B7485_16605-1151.149711peptidase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16830TCRTETA290.028 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 29.4 bits (66), Expect = 0.028
Identities = 36/239 (15%), Positives = 76/239 (31%), Gaps = 18/239 (7%)

Query: 158 SHMQLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFS 217
H + AAL+ + L L + + L+ A F+ R
Sbjct: 159 PHAPFFAAAALNGLNFLTGCFLLPES---HKGERRPLRREALNPLASFRWARGMTVVAAL 215

Query: 218 MLLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLF-ILTIPFFLS 276
M + +Q+ F +D + + I ++ I +L + +
Sbjct: 216 MAVFFIMQLVGQVPAALWVIFGEDRFHWDATTI---GISLAAFGILHSLAQAMITGPVAA 272

Query: 277 RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKE 336
R G + +M+ ++A + L A+ + ++V + + + ++
Sbjct: 273 RLGERRALMLGMIADGTGYILLAF-----ATRGWMAFPIMVLLASGGIGMPALQAMLSRQ 327

Query: 337 VSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQ-TVWLIFAGYSVVL 394
V + QG +T+ L IV + IT W W+ A ++
Sbjct: 328 VDEERQGQLQGSLAALTS-----LTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLC 381


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16855SALSPVBPROT250.038 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 25.1 bits (54), Expect = 0.038
Identities = 10/27 (37%), Positives = 13/27 (48%)

Query: 40 RAMAAELALWARGRHTQFDPTPPPPPV 66
R +A E + R P PPPPP+
Sbjct: 348 RTLAYEGDGYRRAPVNNMMPPPPPPPM 374


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16860RTXTOXIND300.016 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.2 bits (68), Expect = 0.016
Identities = 14/88 (15%), Positives = 27/88 (30%), Gaps = 5/88 (5%)

Query: 17 PEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMST-EIARLKR 75
PE + + + R SL + Q W QNQ+ E L+ E +
Sbjct: 166 PELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTW----QNQKYQKELNLDKKRAERLTVLA 221

Query: 76 QLAERDEELAILPKGRDILREAPEMKYV 103
++ + + D + +
Sbjct: 222 RINRYENLSRVEKSRLDDFSSLLHKQAI 249


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_16865PREPILNPTASE270.024 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 27.1 bits (60), Expect = 0.024
Identities = 14/66 (21%), Positives = 27/66 (40%), Gaps = 1/66 (1%)

Query: 75 IGGGDVKLLTVLSLAIDEHELANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISL 134
+G GD KLL L + L L+ + GA + + +L +P+ +++
Sbjct: 211 MGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMGIGLILLRNHHQS-KPIPFGPYLAI 269

Query: 135 AFLLTY 140
A +
Sbjct: 270 AGWIAL 275


87B7485_17445B7485_17485N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_174450171.764766fimbrial protein
B7485_17455012-1.921041rRNA (cytidine-2'-O-)-methyltransferase
B7485_17460-115-1.054164penicillin-binding protein activator
B7485_17465-1180.502220YraN family protein
B7485_17470-1191.431806phosphoheptose isomerase
B7485_17475-1191.765993osmotically-inducible protein OsmY
B7485_17480-1181.580203permease
B7485_17485-2181.992803hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_17815FIMBRIALPAPF290.022 Escherichia coli: P pili tip fibrillum papF protein...
		>FIMBRIALPAPF#Escherichia coli: P pili tip fibrillum papF protein

signature.
Length = 167

Score = 28.9 bits (64), Expect = 0.022
Identities = 41/160 (25%), Positives = 67/160 (41%), Gaps = 21/160 (13%)

Query: 208 VKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTS 267
V+++I+GN+ P C IN G I V+FG IN + V +I+ C S
Sbjct: 21 VQINIRGNVYIP-PCTINNGQNIVVDFGNINPEHVDNSRG------EVTKNISISCPYKS 73

Query: 268 KIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNG------ 321
SL +++ G T V Q N++A N+ GI + G + NG
Sbjct: 74 ---GSLWIKVTGNTMGVGQNNVLA-----TNITHFGIALYQGKGMSTPLTLGNGSGNGYR 125

Query: 322 ILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVM 361
+ + T + P G L G F+ TA+++++
Sbjct: 126 VTAGLDTARSTFTFTSVPFRNGSGILNGGDFRTTASMSMI 165


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_17825IGASERPTASE300.029 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.4 bits (68), Expect = 0.029
Identities = 41/266 (15%), Positives = 83/266 (31%), Gaps = 15/266 (5%)

Query: 277 QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQPV 336
Q G E + T+ E + Q V S QP A+P
Sbjct: 1087 QSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPA 1146

Query: 337 PVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELL 396
+ P + + N +A+ + + S + V + +++N
Sbjct: 1147 RENDPTVNIKEPQSQTNTTAD--TEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTP 1204

Query: 397 KSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPR---SA 453
+ P + +R ++ + E A D+ A L +
Sbjct: 1205 ATTQPTVNSESSNKPKNRHRRSVRSVPHNVE----PATTSSNDRSTVALCDLTSTNTNAV 1260

Query: 454 LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMT 513
L D A A ++ L G + Q S+L G + ++ + + ++
Sbjct: 1261 LSDARAKA---QFVALNVGKAVSQHI---SQLEMNNEGQYNVWVSNTSMNKNYSSSQYRR 1314

Query: 514 TNNPTLQTTPTDDQFTNNGGRVDAVY 539
++ + QT DQ +N ++ V+
Sbjct: 1315 FSSKSTQTQLGWDQTISNNVQLGGVF 1340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_17835RTXTOXINA280.036 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 27.6 bits (61), Expect = 0.036
Identities = 26/111 (23%), Positives = 44/111 (39%), Gaps = 22/111 (19%)

Query: 42 NKILCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIA-------NDRL 94
K+L GN + A T + IA + V AI+ D+
Sbjct: 277 TKVL--GNVGKGISQYIIAQRAAQGLSTSAAAAGLIA----SAVTLAISPLSFLSIADKF 330

Query: 95 HD----EVYAKQVRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVA 141
E Y+++ + LG+ GD LLA + A++A++T T++A
Sbjct: 331 KRANKIEEYSQRFKKLGYDGDSLLAAFHKETG-----AIDASLTTISTVLA 376


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_17850NUCEPIMERASE290.014 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 29.0 bits (65), Expect = 0.014
Identities = 8/22 (36%), Positives = 13/22 (59%)

Query: 4 VLITGATGLVGGHLLRMLINEP 25
L+TGA G +G H+ + L+
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAG 24


88B7485_17910B7485_17945N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_179105340.841642outer membrane-stress sensor serine
B7485_179156391.368519malate dehydrogenase
B7485_179205330.785741arginine repressor
B7485_179254300.761412hypothetical protein
B7485_17935122-1.074294hypothetical protein
B7485_17940-216-1.429335p-hydroxybenzoic acid efflux pump subunit AaeB
B7485_17945-213-1.842894p-hydroxybenzoic acid efflux pump subunit AaeA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18265V8PROTEASE538e-10 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 52.7 bits (126), Expect = 8e-10
Identities = 31/160 (19%), Positives = 59/160 (36%), Gaps = 26/160 (16%)

Query: 77 RTLGSGVIMDQRGYIITNKHVINDADQIIVALQ------------DGRVFEALLVGSDSL 124
+ SGV++ + ++TNKHV++ AL+ +G +
Sbjct: 101 TFIASGVVVG-KDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGE 159

Query: 125 TDLAVLKI-------NATGGLPTIPINARRVPHIGDVVLAIGNPYNLGQTITQGIISATG 177
DLA++K + + ++ + + G P + T + G
Sbjct: 160 GDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGD-KPVATMW--ESKG 216

Query: 178 RIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINT 217
+I + +Q D S GNSG + N E++GI+
Sbjct: 217 KI---TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHW 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18270DHBDHDRGNASE280.045 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 28.1 bits (62), Expect = 0.045
Identities = 37/167 (22%), Positives = 61/167 (36%), Gaps = 27/167 (16%)

Query: 3 VAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGED 62
+ GAA GIG+A+A L G+ ++ D P V S A + F +
Sbjct: 11 AFITGAAQGIGEAVARTL---ASQGAHIAAVDYNP-EKLEKVVSSLKAEARHAEAFPADV 66

Query: 63 ATPA------------LEGADVVLISAGVARK------PGMDRSDLFNVNAGIVKNLVQQ 104
A + D+++ AGV R + F+VN+ V N +
Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126

Query: 105 VAKNCPK----ACIGIITNPVNTT-VAIAAEVLKKAGVYDKNKLFGV 146
V+K + + + +NP ++AA KA K G+
Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGL 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18275ARGREPRESSOR1694e-57 Bacterial arginine repressor signature.
		>ARGREPRESSOR#Bacterial arginine repressor signature.

Length = 149

Score = 169 bits (430), Expect = 4e-57
Identities = 44/141 (31%), Positives = 71/141 (50%), Gaps = 5/141 (3%)

Query: 15 KALLKEEKFSSQGEIVAALQEQGFDNINQSKVSRMLTKFGAVRTRNAKMEMVYCLPAELG 74
+ ++ + +Q E+V L++ G+ N+ Q+ VSR + + V+ Y LPA+
Sbjct: 11 REIITANEIETQDELVDILKKDGY-NVTQATVSRDIKELHLVKVPTNNGSYKYSLPADQR 69

Query: 75 VPTTSSPLKNLV---LDIDYNDAVVVIHTSPGAAQLIARLLDSLGKAEGILGTIAGDDTI 131
S ++L+ + ID ++V+ T PG AQ I L+D+L E I+GTI GDDTI
Sbjct: 70 FNPLSKLKRSLMDAFVKIDSASHLIVLKTMPGNAQAIGALMDNLDWEE-IMGTICGDDTI 128

Query: 132 FTTPANGFTVKDLYEAILELF 152
K + + ILEL
Sbjct: 129 LIICRTHDDTKVVQKKILELL 149


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18295RTXTOXIND535e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 53.3 bits (128), Expect = 5e-10
Identities = 28/163 (17%), Positives = 59/163 (36%), Gaps = 16/163 (9%)

Query: 6 RKFSRTAITVVLVILAFIAIFNAWVYYTE----SPWTRDARFSADVVAIAPDVSGLITQV 61
SR V I+ F+ I + + S I P + ++ ++
Sbjct: 51 TPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEI 110

Query: 62 NVHDNQLVKKGQILFTIDQPR-------YQKALEEAQADVAYYQVLAQEKRQEAGRRNRL 114
V + + V+KG +L + Q +L +A+ + YQ+L++ E + L
Sbjct: 111 IVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRS--IELNKLPEL 168

Query: 115 GVQAMSREEIDQANNVL---QTVLHQLAKAQATRDLAKLDLER 154
+ + VL + Q + Q + +L+L++
Sbjct: 169 KLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDK 211



Score = 51.4 bits (123), Expect = 2e-09
Identities = 28/147 (19%), Positives = 54/147 (36%), Gaps = 15/147 (10%)

Query: 100 LAQEKRQEAGRRNRLGVQ-AMSREEIDQANNVLQT-VLHQLAKAQAT-------RDLAKL 150
E R + ++ + ++EE + + +L +L + +
Sbjct: 264 AVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEE 323

Query: 151 DLERTVIRAPADGWVTNLNVYT-GEFITRGSTAVALVKQNSFY-VLAYMEETKLEGVRPG 208
+ +VIRAP V L V+T G +T T + +V ++ V A ++ + + G
Sbjct: 324 RQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVG 383

Query: 209 YRAEIT----PLGSNKVLKGTVDSVAA 231
A I P L G V ++
Sbjct: 384 QNAIIKVEAFPYTRYGYLVGKVKNINL 410


89B7485_18375B7485_18400N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_18375-2161.475481prepilin peptidase
B7485_18380-3120.369263bacterioferritin
B7485_18385-212-0.251896bacterioferritin-associated ferredoxin
B7485_18390-115-1.023770ferredoxin
B7485_18395019-2.398286translation elongation factor EF-Tu 1
B7485_18400-115-3.283362elongation factor G
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18720PREPILNPTASE1428e-45 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 142 bits (360), Expect = 8e-45
Identities = 65/142 (45%), Positives = 84/142 (59%), Gaps = 2/142 (1%)

Query: 4 TLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIVGY 63
TL L+L L AL F D LLPD+ T PLLW GLLF + L DA+ GA+ GY
Sbjct: 134 TLAALLLTWVLVALTFI-DLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMAGY 192

Query: 64 GTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLM 123
+YW +++L KEG+GYGD K LAALGAW W LP +V L +S + IGL++
Sbjct: 193 LVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALP-IVLLLSSLVGAFMGIGLIL 251

Query: 124 RGKESLKNPLPFGPFLAAAGFV 145
P+PFGP+LA AG++
Sbjct: 252 LRNHHQSKPIPFGPYLAIAGWI 273


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18725HELNAPAPROT383e-06 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 38.3 bits (89), Expect = 3e-06
Identities = 19/103 (18%), Positives = 43/103 (41%), Gaps = 10/103 (9%)

Query: 44 EYHESIDEMKHADKYIERILFLEGIPN--LQDLGKL------GIGEDVEEMLQSDLRLEL 95
E ++ E D ER+L + G P +++ + G EM+Q+ +
Sbjct: 52 ELYDHAAE--TVDTIAERLLAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYK 109

Query: 96 EGAKDLREAIAYADSVHDYVSRDMMIEILADEEGHIDWLETEL 138
+ + + + I A+ D + D+ + ++ + E + L + L
Sbjct: 110 QISSESKFVIGLAEENQDNATADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18740TCRTETOQM803e-18 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 79.5 bits (196), Expect = 3e-18
Identities = 57/198 (28%), Positives = 87/198 (43%), Gaps = 13/198 (6%)

Query: 13 VNVGTIGHVDHGKTTLTAAI------TTVLAKTYGGAARAFDQIDNAPEEKARGITINTS 66
+N+G + HVD GKTTLT ++ T L G R DN E+ RGITI T
Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRT----DNTLLERQRGITIQTG 59

Query: 67 HVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQV 126
+ +D PGH D++ + + +DGAIL+++A DG QTR R++
Sbjct: 60 ITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKM 119

Query: 127 GVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWE 186
G+P I F+NK D + L V +++E LS + + +W+
Sbjct: 120 GIP-TIFFINKIDQNGID--LSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWD 176

Query: 187 AKILELAGFLDSYIPEPE 204
I L+ Y+
Sbjct: 177 TVIEGNDDLLEKYMSGKS 194


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18745TCRTETOQM6110.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 611 bits (1578), Expect = 0.0
Identities = 178/698 (25%), Positives = 304/698 (43%), Gaps = 81/698 (11%)

Query: 9 RYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAAT 68
+ NIG+ AH+DAGKTT TE +L+ +G ++G V G D E++RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 69 TAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWR 128
+ W ++NIIDTPGH+DF EV RS+ VLDGA+++ A GVQ Q+ ++
Sbjct: 62 SFQWEN-------TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFH 114

Query: 129 QANKYKVPRIAFVNKMDRVGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKM 188
K +P I F+NK+D+ G + V IK +L A V Q V M
Sbjct: 115 ALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ----------KVELYPNM 164

Query: 189 KAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEI 248
N+ +++Q ++ E +++L+EKY+ G+ L E+
Sbjct: 165 CVTNFTESEQ------------------------WDTVIEGNDDLLEKYMSGKSLEALEL 200

Query: 249 KGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDDGKDTPAE 308
+ R N + V GSA N G+ +++ + + S
Sbjct: 201 EQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH----------------- 243

Query: 309 RHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHA 368
FKI L + R+YSGV++ D+V S K + + +
Sbjct: 244 ---RGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEK-EKIKITEMYTSIN 299

Query: 369 NKREEIKEVRAGDIAAAIG----LKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKT 424
+ +I + +G+I L V GDT P ER+E P P++ VEP
Sbjct: 300 GELCKIDKAYSGEIVILQNEFLKLNSV-LGDTKLLPQR----ERIENPLPLLQTTVEPSK 354

Query: 425 KADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVEANVG 484
+E + AL ++ DP R + D +++ I++ +G++ +++ ++ +++VE +
Sbjct: 355 PQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIK 414

Query: 485 KPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGV 544
+P V Y E +K E + + + + + PL GS G ++ + + G
Sbjct: 415 EPTVIYMERPLKK---AEYTIHIEVPPNPFWASIGLSVSPLPLGS---GMQYESSVSLGY 468

Query: 545 IPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEG 604
+ + AV +GI+ + G L G+ V D I +G Y+ S+ F++ A I ++
Sbjct: 469 LNQSFQNAVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQV 527

Query: 605 FKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMF 664
KKA LLEP + ++ P+E D + + + + V + E+P +
Sbjct: 528 LKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQ 587

Query: 665 GYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEAR 702
Y + L T GR+ E Y + V + R
Sbjct: 588 EYRSDLTFFTNGRSVCLTELKGYHVT---TGEPVCQPR 622


90B7485_18435B7485_18530N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_18435118-2.362121hypothetical protein
B7485_18440119-2.794673FKBP-type peptidyl-prolyl cis-trans isomerase
B7485_18445119-2.697839protein SlyX
B7485_18450119-2.593700peptidylprolyl isomerase
B7485_18455223-3.290782hypothetical protein
B7485_18495220-3.167123glutathione-regulated potassium-efflux system
B7485_18500223-1.852792glutathione-regulated potassium-efflux system
B7485_18505023-2.269881hypothetical protein
B7485_18515022-1.840949ABC transporter ATP-binding protein
B7485_18520-120-1.817519hydrolase
B7485_18525-220-1.504690hypothetical protein
B7485_18530-318-1.332030phosphoribulokinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18775ACRIFLAVINRP290.022 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.0 bits (65), Expect = 0.022
Identities = 14/62 (22%), Positives = 29/62 (46%), Gaps = 1/62 (1%)

Query: 160 ASSVEDLVTQTLEFTIEEVNADRNV-SNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI 218
A +V+D VTQ +E + ++ + S + + + L + D A QV ++L +
Sbjct: 54 AQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQL 113

Query: 219 SK 220
+
Sbjct: 114 AT 115


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18780INFPOTNTIATR1332e-40 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 133 bits (337), Expect = 2e-40
Identities = 80/226 (35%), Positives = 124/226 (54%), Gaps = 9/226 (3%)

Query: 28 AAKPATTADSKASFKNDDQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAF 87
A A A S D K +Y++GA LG K + GI ++ D L G+QD
Sbjct: 14 AMSTAMAATDATSLTTDKDKLSYSIGADLG-------KNFKNQGIDINPDVLAKGMQDGM 66

Query: 88 A-DKSKLSDQEIEQTLQAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSST 146
+ + L++++++ L F+ + + A+ K A +N+AKG + + G+ +
Sbjct: 67 SGAQLILTEEQMKDVLSKFQKDLMAKRSAEFNKKAEENKAKGDAFLSANKSKPGIVVLPS 126

Query: 147 GLVYQVVEAGKGEAPKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGL 206
GL Y++++AG G P SDTV V Y GTLIDG FD++ G+P +F++ VIPGWTE L
Sbjct: 127 GLQYKIIDAGTGAKPGKSDTVTVEYTGTLIDGTVFDSTEKAGKPATFQVSQVIPGWTEAL 186

Query: 207 KNIKKGGKIKLVIPPELAYGKAGVPG-IPPNSTLVFDVELLDVKPA 251
+ + G ++ +P +LAYG V G I PN TL+F + L+ VK A
Sbjct: 187 QLMPAGSTWEVFVPADLAYGPRSVGGPIGPNETLIFKIHLISVKKA 232


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_1880060KDINNERMP300.021 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 30.3 bits (68), Expect = 0.021
Identities = 13/69 (18%), Positives = 29/69 (42%), Gaps = 6/69 (8%)

Query: 230 TAIDPFKGLLLG---LFFISVGMSLNLGVLYTHL-LWVVISVVVLVAVKILVLYLLARLY 285
A+ P L + L+FIS + L +++ + W +++ V+ ++ L
Sbjct: 318 AAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKA-- 375

Query: 286 GVRSSERMQ 294
S +M+
Sbjct: 376 QYTSMAKMR 384


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18805ISCHRISMTASE320.001 Isochorismatase signature.
		>ISCHRISMTASE#Isochorismatase signature.

Length = 312

Score = 31.9 bits (72), Expect = 0.001
Identities = 32/135 (23%), Positives = 51/135 (37%), Gaps = 16/135 (11%)

Query: 12 YAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLREHEVIVFQH-- 69
Y P + D N+V P + + +HD+ ++ D F L + +
Sbjct: 9 YQMPTASDMPQNKVSWVPDPNRAVLLIHDMQNYFVDAFTAGASPVTELSANIRKLKNQCV 68

Query: 70 ----PLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAGKYWRNVITTGEPESA------Y 119
P+ + P DR L F GPG N +G Y +IT PE +
Sbjct: 69 QLGIPVVYTAQPGSQNP-DDRALLTDFW-GPGLN--SGPYEEKIITELAPEDDDLVLTKW 124

Query: 120 RYDALNRYPMSDVLR 134
RY A R + +++R
Sbjct: 125 RYSAFKRTNLLEMMR 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18815GPOSANCHOR330.005 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 32.7 bits (74), Expect = 0.005
Identities = 28/152 (18%), Positives = 54/152 (35%), Gaps = 22/152 (14%)

Query: 504 KVEPFDGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKE 563
+ D + ++ E + + ++ R+ +R R + L E
Sbjct: 272 AMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAE 331

Query: 564 IARLEKEME---------------------KLNAQLAQAEEKLGDSELYDQSRKAELTAC 602
+LE++ + +L A+ + EE+ SE QS + +L A
Sbjct: 332 HQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDAS 391

Query: 603 LQQQASAKSGLEECEMAWLEAQEQLEQMLLEG 634
+ + + LEE L A E+L + L E
Sbjct: 392 REAKKQVEKALEEANSK-LAALEKLNKELEES 422



Score = 32.0 bits (72), Expect = 0.008
Identities = 13/125 (10%), Positives = 39/125 (31%), Gaps = 7/125 (5%)

Query: 513 EDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIARLEKEME 572
+ + ++ + E A A + D ++ + +++
Sbjct: 127 KALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFST-------ADSAKIK 179

Query: 573 KLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQEQLEQMLL 632
L A+ A E + + E + TA + + ++ + ++ LE +
Sbjct: 180 TLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMN 239

Query: 633 EGQSN 637
++
Sbjct: 240 FSTAD 244


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_18830PF07299320.002 Fibronectin-binding protein (FBP)
		>PF07299#Fibronectin-binding protein (FBP)

Length = 219

Score = 31.8 bits (72), Expect = 0.002
Identities = 10/46 (21%), Positives = 21/46 (45%), Gaps = 2/46 (4%)

Query: 71 PEANDFGLLEQTFIEYGQSGKGKSRKYLHTYDEAVPWNQVPGTFTP 116
P+ + + E ++ KG SRK++ ++ + + GTF
Sbjct: 112 PDMEELDMKELSY--LSWIDKGSSRKFIIAKNDKNKFVGLQGTFQS 155


91B7485_19040B7485_19075N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_19040-1102.364891N-acetyltransferase
B7485_19045-1102.206429gamma-glutamyltransferase
B7485_19050-1121.572222hypothetical protein
B7485_19055-310-0.063447glycerophosphoryl diester phosphodiesterase
B7485_19060-311-0.350774sn-glycerol 3-phosphate ABC transporter
B7485_19070-1131.074644sn-glycerol 3-phosphate ABC transporter
B7485_19075-1141.259269sn-glycerol-3-phosphate ABC transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19320SACTRNSFRASE371e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 37.2 bits (86), Expect = 1e-05
Identities = 21/92 (22%), Positives = 33/92 (35%), Gaps = 16/92 (17%)

Query: 55 VACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMREMIE------MCD 108
+ ++ + +G + I + + D + D R K GV +AL+ + IE C
Sbjct: 69 LYYLENNCIGRIKIR-SNWNGYALIEDIAVAKDYRKK--GVGTALLHKAIEWAKENHFCG 125

Query: 109 NWLRVDRIELTVFVDNAPAIKVYKKFGFEIEG 140
L I N A Y K F I
Sbjct: 126 LMLETQDI-------NISACHFYAKHHFIIGA 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19325NAFLGMOTY320.007 Sodium-type flagellar protein MotY precursor signature.
		>NAFLGMOTY#Sodium-type flagellar protein MotY precursor signature.

Length = 293

Score = 31.6 bits (71), Expect = 0.007
Identities = 27/82 (32%), Positives = 37/82 (45%), Gaps = 17/82 (20%)

Query: 275 RTPISGDYRGYQVYSMPPPSSGGIHIVQILNI--LENFDMKKYGF-GSADAMQIMAEAEK 331
R P+ G+ R + SMPPP G H +I N+ + FD G+ G A I++E EK
Sbjct: 77 RRPM-GETRNVSLISMPPPWRPGEHADRITNLKFFKQFD----GYVGGQTAWGILSELEK 131

Query: 332 YAYADRSEYLGDPDFVKVPWQA 353
Y P F WQ+
Sbjct: 132 GRY---------PTFSYQDWQS 144


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19335PF04619280.017 Dr-family adhesin
		>PF04619#Dr-family adhesin

Length = 160

Score = 28.4 bits (63), Expect = 0.017
Identities = 12/60 (20%), Positives = 22/60 (36%), Gaps = 4/60 (6%)

Query: 29 VGAKYGHKMIEFDAKLSKDGEIFLLHDDNLERTSNGWGVAGELNWQD----LLRVDAGSW 84
+G ++ D + G+ FL+ D+N ++ W + D GSW
Sbjct: 70 LGCDARQVALKADTDNFEQGKFFLISDNNRDKLYVNIRPTDNSAWTTDNGVFYKNDVGSW 129


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19355MALTOSEBP392e-05 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 39.3 bits (91), Expect = 2e-05
Identities = 41/160 (25%), Positives = 68/160 (42%), Gaps = 14/160 (8%)

Query: 134 GHLLSQPFNSSTPVLYYNKDAFKKAGLDPEQPPKTWQDLADYSAKLKASGIKCGYASGWQ 193
G L++ P L YNKD L P PPKTW+++ +LKA G + +
Sbjct: 127 GKLIAYPIAVEALSLIYNKD------LLP-NPPKTWEEIPALDKELKAKGKSALMFNLQE 179

Query: 194 GWIQLENFSAWNGLPFASKNNGFDGTDAVLEF--NKPEQVKHIAMLEEMNKKGDFSYVGR 251
+ +A G F +N +D D ++ K + +++ + D Y
Sbjct: 180 PYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDY--- 236

Query: 252 KDESTEKFYNGDCAMTTASSGSLANIREYAKFNYGVGMMP 291
+ F G+ AMT + +NI + +K NYGV ++P
Sbjct: 237 -SIAEAAFNKGETAMTINGPWAWSNI-DTSKVNYGVTVLP 274


92B7485_19230B7485_19260N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_19230-1160.515983nickel import ATP-binding protein NikE
B7485_19235-2140.917116nickel-responsive transcriptional regulator
B7485_19240-1130.311619toxin-antitoxin system HicB family antitoxin
B7485_19245-2110.974880type II toxin-antitoxin system HicA family
B7485_19250-1111.218324inner membrane transport permease YhhJ
B7485_192550121.192386ABC transporter ATP-binding protein
B7485_19260-1111.605470hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19495HTHFIS290.018 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.4 bits (66), Expect = 0.018
Identities = 10/34 (29%), Positives = 19/34 (55%)

Query: 25 QAVLNNVSLTLKSGETVALLGRSGCGKSTLARLL 58
Q + ++ +++ T+ + G SG GK +AR L
Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19515ABC2TRNSPORT505e-09 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 49.9 bits (119), Expect = 5e-09
Identities = 41/171 (23%), Positives = 73/171 (42%), Gaps = 7/171 (4%)

Query: 200 REREHGTVEHLLVMPITPFEIMMAKI-WSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPL 258
R T E +L + +I++ ++ W+ L +G+ +V G + L
Sbjct: 93 RMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGY----TQWLSLL 148

Query: 259 FMLGV-ALSLFATTSIGIFMGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQD 317
+ L V AL+ A S+G+ + +A S LV+ P+ LSG P + +P + Q
Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208

Query: 318 IMLTMPTTHFVSLAQAILYRGAGFEIVWPQFLTLMAIGGAFF-TIALLRFR 367
+P +H + L + I+ ++ + I FF + ALLR R
Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTALLRRR 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19520PF05272300.045 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 30.4 bits (68), Expect = 0.045
Identities = 9/26 (34%), Positives = 14/26 (53%)

Query: 37 ARCMVGLIGPDGVGKSSLLSLISGAR 62
V L G G+GKS+L++ + G
Sbjct: 595 FDYSVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19525RTXTOXIND844e-20 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 84.5 bits (209), Expect = 4e-20
Identities = 71/408 (17%), Positives = 139/408 (34%), Gaps = 81/408 (19%)

Query: 6 RHLAWWVVGALAVAAVVAWWLLRPAGVP-EGFAVSNGRIEATEVDIASKIAGRIDTILVK 64
R +A++++G L +A +++ G +GR + I + I+VK
Sbjct: 58 RLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKE----IKPIENSIVKEIIVK 113

Query: 65 EGQFVREGEVLAKMDTRV----------------LQEQRLEAI----------------- 91
EG+ VR+G+VL K+ L++ R + +
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 92 -------------------AQIKEAQSAVAAAQALLEQRQSETRAAQSLVNQRQAELDSV 132
Q Q+ + L+++++E + +N+ +
Sbjct: 174 PYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVE 233

Query: 133 AKRHTRSRSLAQRGAISAQQLDDDRAAAESARAALESAKAQVSASKAAIEAARTNIIQ-- 190
R SL + AI+ + + A L K+Q+ ++ I +A+
Sbjct: 234 KSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVT 293

Query: 191 -----------AQTRVEAAQATERRIAADID--DSELKAPRDGRV-QYRVAEPGEVLAAG 236
QT T + S ++AP +V Q +V G V+
Sbjct: 294 QLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTA 353

Query: 237 GRVLNMVDLSDVY-MTFFLPTEQAGTLKLGGEARLILDAAPDLRIPATISFVASVAQFTP 295
++ +V D +T + + G + +G A + ++A P R V V
Sbjct: 354 ETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYG---YLVGKVKNINL 410

Query: 296 KTVETSDERLKLMFRVKARIPPELLQQHLEYV--KTGLPGVAWVRVNE 341
+E D+RL L+F V I L + + +G+ A ++
Sbjct: 411 DAIE--DQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGM 456


93B7485_19525B7485_19555N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_19525137-10.424244OmpA family lipoprotein
B7485_19530246-14.596977molybdopterin guanine dinucleotide-containing
B7485_19535444-14.711845N-acetyltransferase
B7485_19540240-11.667745DNA-3-methyladenine glycosylase I
B7485_19545337-10.271303autotransporter outer membrane beta-barrel
B7485_19550334-8.168832hypothetical protein
B7485_19555333-7.459227oxalate/formate antiport family MFS transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19805OMPADOMAIN1111e-31 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 111 bits (280), Expect = 1e-31
Identities = 40/122 (32%), Positives = 61/122 (50%), Gaps = 11/122 (9%)

Query: 97 LNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEY--PKTAVNVIGYTDSTGGHDLNMRLS 154
+ ++V F+ + A LKP G L + L +V V+GYTD G N LS
Sbjct: 215 FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS 274

Query: 155 QQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGK---------AQNRRVEITL 205
++RA SV LI++G+ A +I +G+G +NP+ N+ K A +RRVEI +
Sbjct: 275 ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334

Query: 206 SP 207

Sbjct: 335 KG 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19815SACTRNSFRASE355e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 34.9 bits (80), Expect = 5e-05
Identities = 16/52 (30%), Positives = 22/52 (42%), Gaps = 5/52 (9%)

Query: 76 VAPKAVRRGIGKALMQYV-----QQRYPHLMLEVYQKNQPAIDFYRAQGFHI 122
VA ++G+G AL+ + + LMLE N A FY F I
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFII 148


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19825ECOLNEIPORIN280.039 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 27.8 bits (62), Expect = 0.039
Identities = 19/90 (21%), Positives = 37/90 (41%), Gaps = 13/90 (14%)

Query: 119 SMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQISYNQQFGENIWKAQSGLS 178
S+ + D+ + H S + + + R G++ P ++SY F +
Sbjct: 228 SVAVQQQDAKLV-EENYSHNSQTEVAATLAYRFGNVTP--RVSYAHGFKGSF-------- 276

Query: 179 RMTATNQNGNWLDVTVGADMLLNQNIAAYA 208
ATN N ++ V VGA+ ++ +A
Sbjct: 277 --DATNYNNDYDQVVVGAEYDFSKRTSALV 304


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_19835TCRTETA432e-06 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 42.9 bits (101), Expect = 2e-06
Identities = 47/275 (17%), Positives = 94/275 (34%), Gaps = 32/275 (11%)

Query: 44 PVSQVAFSFGLL----SLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNL 99
+ V +G+L +L + V G L +RFG + V + S + + + A + L
Sbjct: 37 HSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFL 96

Query: 100 MMLWLS---AGVLVGLADGAGYLL----TLSNCVKWFPERKGLISAFAIGSYGLGSLGFK 152
+L++ AG+ AG + + F + LG
Sbjct: 97 WVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGG---- 152

Query: 153 FIDTQLLETVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMR 212
L+ F A+ + + G L+ ++ K E + R
Sbjct: 153 -----LMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWAR 207

Query: 213 --KPQYWMLAVMFLTACMSG----LYVIGVAKDIAQSLAHLDVVSAANAVTVISIAN-LS 265
++AV F+ + L+VI + H D + ++ I + L+
Sbjct: 208 GMTVVAALMAVFFIMQLVGQVPAALWVI-----FGEDRFHWDATTIGISLAAFGILHSLA 262

Query: 266 GRLVLGILSDKIARIRVITIGQVISLVGMAALLFA 300
++ G ++ ++ R + +G + G L FA
Sbjct: 263 QAMITGPVAARLGERRALMLGMIADGTGYILLAFA 297



Score = 36.0 bits (83), Expect = 2e-04
Identities = 37/155 (23%), Positives = 64/155 (41%), Gaps = 9/155 (5%)

Query: 241 AQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFA 300
AH ++ A A+ + A + G L SD+ R V+ + + V A + A
Sbjct: 39 NDVTAHYGILLALYALMQFACAPVLGAL-----SDRFGRRPVLLVSLAGAAVDYAIMATA 93

Query: 301 PLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSIFGSIIA 360
P V + I VA G T V + +++ + A+++G + FG G + G ++
Sbjct: 94 PFLWVLYIGRI--VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG 151

Query: 361 SLFGGF--YVTFYVIFALLILSLALSTTIRQPEQK 393
L GGF + F+ AL L+ + K
Sbjct: 152 GLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHK 186


94B7485_20450B7485_20485N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_20450-116-4.870435MFS transporter
B7485_20455126-8.196620aerobactin synthase IucA
B7485_20460227-9.285457N-acetyltransferase
B7485_20465337-11.957066IucA/IucC family siderophore biosynthesis
B7485_20470446-15.832971lysine 6-monooxygenase
B7485_20475446-16.612974TonB-dependent siderophore receptor
B7485_20480240-14.084383hypothetical protein
B7485_20485334-12.234223serine protease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20740TCRTETA485e-08 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 47.5 bits (113), Expect = 5e-08
Identities = 81/375 (21%), Positives = 135/375 (36%), Gaps = 41/375 (10%)

Query: 20 FSAGLLGIGQNGLLVVLPVLVIQTNLSLSV---WAALLMLGSMLFLPSSPWWGKQISRTG 76
+ L +G ++ VLP L+ S V + LL L +++ +P G R G
Sbjct: 12 STVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFG 71

Query: 77 SKPVVLWALGGYGISFTLLGLGSVLMATSAITTAVGLGILIIARIAYGLTVSAMVPACQV 136
+PV+L +L G + + ++ L +L I RI G+T + A
Sbjct: 72 RRPVLLVSLAGAAVDYAIMATAPFLW------------VLYIGRIVAGITGATGAVAGAY 119

Query: 137 WALQRAGEGNRMAALATISSGLSCGRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLL 196
A R +S+ G + GP+ M P AP A L L
Sbjct: 120 IA-DITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGC 178

Query: 197 RL------PGTPPQPTPECKSVSLKRDCLPYLLCAILLAAAVSMMQLGLSPAL------T 244
L P ++ R + A L+A M +G PA
Sbjct: 179 FLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGE 238

Query: 245 RQFATDTTAISQQVAWLLGLSAVAALIAQFGVLRPQRLTPVALLLSAGVLMSGGLAIMLS 304
+F D T I +A L ++A + G + + AL+L +G + + +
Sbjct: 239 DRFHWDATTIGISLAAFGILHSLAQAMI-TGPVAARLGERRALMLGMIADGTGYILLAFA 297

Query: 305 EQLWLFYPGCAVLSFGAALATPAYQLLLNDKLADGAGAGWLATSHTLGYGLCALLVPLVS 364
+ W+ +P +L+ G + PA Q +L+ + D G L L L S
Sbjct: 298 TRGWMAFPIMVLLASG-GIGMPALQAMLS-RQVDEERQGQLQ----------GSLAALTS 345

Query: 365 KTGVAIALIMAALFA 379
T + L+ A++A
Sbjct: 346 LTSIVGPLLFTAIYA 360


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20745PF04183339e-111 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 339 bits (872), Expect = e-111
Identities = 104/480 (21%), Positives = 178/480 (37%), Gaps = 46/480 (9%)

Query: 58 ELLIPLDEQKSLHFRVAYFSPTQHHRF-----AFPARLVTASGSYPVDFTTLSRLIIDKL 112
E + + Q + + P RF + + A D L++ ++ +L
Sbjct: 24 EQVFHAESQGDDRYCIN--LPGAQWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQL 81

Query: 113 RHQLFLPVPLCETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHP 172
+ L + Q + + + Q + AR +A LN + Q LL+GH
Sbjct: 82 KQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASDLINLNA-DRLQCLLSGHPKFV 140

Query: 173 APKSHEPFNRREAERYLPDMAPHFPLRWFSVDKTQIAGES-LHLNLQQRLTRFAAENAPQ 231
K + + ERY P+ A F L W +V + + +++ Q LT A PQ
Sbjct: 141 FNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRCDNEMDIHQLLT---AAMDPQ 197

Query: 232 LLNELS--------DNQWLF-PLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTT 282
S D+ WL P+HPWQ + + + A+G + LGE G WL
Sbjct: 198 EFARFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADF-AEGRMVSLGEFGDQWLAQQ 256

Query: 283 SSRSLYCATSRD--MIKFSLSVRLTNSIRTLSVKEVKRGMRLARLAQ----TDGWQMLQ- 335
S R+L A+ R IK L++ T+ R + + + G +R Q TD +
Sbjct: 257 SLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSG 316

Query: 336 ---VRFPTFRVMQEDGWAGLLDLNGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPD 392
+ P + +G+A L + REN ++ VL++ +
Sbjct: 317 AVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDE 376

Query: 393 GGDSLLVSAVKRLSDRLGITVQQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILV 452
L + + DR G+ A W+ + V+ PL+ YG+ L+AH QNI +
Sbjct: 377 NNQPLAGAYI----DRSGLD----AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITL 428

Query: 453 QMLGDLPVGFIYRDCQGSAFMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFA 512
M +P + +D QG M + + E + L++
Sbjct: 429 AMKEGVPQRVLLKDFQGD--MRLVKEEFPEMDSLPQE----VRDVTSRLSADYLIHDLQT 482


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20755PF041838160.0 IucA / IucC family
		>PF04183#IucA / IucC family

Length = 580

Score = 816 bits (2109), Expect = 0.0
Identities = 565/580 (97%), Positives = 571/580 (98%)

Query: 1 MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGAQWRFIAERGIWGWLWI 60
MNHKDWD VNRRLVAKMLSE+EYEQVFHAESQGDD YCINLPGAQWRFIAERGIWGWLWI
Sbjct: 1 MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGAQWRFIAERGIWGWLWI 60

Query: 61 DAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASD 120
DAQTLRC DEPVLAQTLLMQLK VLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASD
Sbjct: 61 DAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYATLLGDLQLLKARRGLSASD 120

Query: 121 LINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYTNTFRLHWLAVKREHMIWRC 180
LINL+ADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY NTFRLHWLAVKREHMIWRC
Sbjct: 121 LINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEYANTFRLHWLAVKREHMIWRC 180

Query: 181 DNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEG 240
DN++DI QLLTAAMDPQEF RFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEG
Sbjct: 181 DNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPLPVHPWQWQQKIATDFIADFAEG 240

Query: 241 RMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASR 300
RMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASR
Sbjct: 241 RMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPLTIYNTSCYRGIPGRYIAAGPLASR 300

Query: 301 WLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLK 360
WLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLK
Sbjct: 301 WLQQVFATDATLVQSGAVILGEPAAGYVSHEGYAALARAPYRYQEMLGVIWRENPCRWLK 360

Query: 361 PDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALI 420
PDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALI
Sbjct: 361 PDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLTQLFRVVVVPLYHLLCRYGVALI 420

Query: 421 AHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEAFPEMDSLPQEVRDVTSRLSADYLIHDL 480
AHGQNITLAMKEGVPQRVLLKDFQGDMRLVKE FPEMDSLPQEVRDVTSRLSADYLIHDL
Sbjct: 421 AHGQNITLAMKEGVPQRVLLKDFQGDMRLVKEEFPEMDSLPQEVRDVTSRLSADYLIHDL 480

Query: 481 QTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMNKHPQMAERFALFSLFRPQIIR 540
QTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYM KHPQM+ERFALFSLFRPQIIR
Sbjct: 481 QTGHFVTVLRFISPLMVRLGVPERRFYQLLAAVLSDYMKKHPQMSERFALFSLFRPQIIR 540

Query: 541 VVLNPVKLTWPDLDGGSRMLPNYLENLQNPLWLVTQEYES 580
VVLNPVKLTWPDLDGGSRMLPNYLE+LQNPLWLVTQEYES
Sbjct: 541 VVLNPVKLTWPDLDGGSRMLPNYLEDLQNPLWLVTQEYES 580


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20775IGASERPTASE834e-20 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 82.8 bits (204), Expect = 4e-20
Identities = 48/214 (22%), Positives = 74/214 (34%), Gaps = 52/214 (24%)

Query: 31 NRKLVATMLSLAVAGTVNA---ANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGD 87
N+K ++L VA + A + +V + + D A+NKG F GAT+V + KN
Sbjct: 3 NKKFKLNFIALTVAYALTPYTEAALVRDDVDYQIFRDFAENKGKFSVGATNVLVKDKNNK 62

Query: 88 KF--SFHN-LSIPDFSGAAAS-GAATAIGGSYSVTVAH-----------------NKKNP 126
+ N + + DFS AT I Y V V H N N
Sbjct: 63 DLGTALPNGIPMIDFSVVDVDKRIATLINPQYVVGVKHVSNGVSELHFGNLNGNMNNGNA 122

Query: 127 QAAETQVYAQSSYKVVDRRNSN-------------------DFEIQRLNKFVVETVGATP 167
+A ++ Y V++ D+ + RL+KFV E
Sbjct: 123 KAHRDVSSEENRYFSVEKNEYPTKLNGKTVTTEDQTQKRREDYYMPRLDKFVTEVAPIEA 182

Query: 168 AETNPTTYSDALERYGIVTSDGSKKIIGFRAGSG 201
+ + +D +K R GSG
Sbjct: 183 STAS---------SDAGTYNDQNKYPAFVRLGSG 207


95B7485_20505B7485_20545N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_20505-114-0.680783MFS transporter
B7485_20510-313-0.408996hypothetical protein
B7485_20515-2131.073827hypothetical protein
B7485_205200130.149429NCS2 family permease
B7485_20525016-0.168250adenine deaminase
B7485_205300170.095486hexose phosphate transporter
B7485_20535018-0.157277MFS transporter family glucose-6-phosphate
B7485_205400140.580903two-component system sensor histidine kinase
B7485_20545-1141.175311DNA-binding response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20800TCRTETA385e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 37.9 bits (88), Expect = 5e-05
Identities = 33/208 (15%), Positives = 71/208 (34%), Gaps = 13/208 (6%)

Query: 33 IIVEFLPVSLLTP----MAQDLGISEGVA---GQSVTVTAFVAMFASLFITQTIQATDRR 85
+ ++ + + L+ P + +DL S V G + + A + + + RR
Sbjct: 14 VALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRR 73

Query: 86 YVVILFAVLLTLSCLLVSFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKA 145
V+++ + +++ A +L IGR G+ G A++ + + +
Sbjct: 74 PVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADITDGDERARH 132

Query: 146 LSVIFGAVSIALVIAAPLGSFLGELIGWRNVFNAAAVMG----VLCIFWIIKSLPSLPGE 201
+ +V LG +G F AAA + + F + +S
Sbjct: 133 FGFMSACFGFGMVAGPVLGGLMGG-FSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRP 191

Query: 202 PSHQKQNTFRLLQRLGVMAGMIAIFMSF 229
+ N + M + A+ F
Sbjct: 192 LRREALNPLASFRWARGMTVVAALMAVF 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20820UREASE403e-05 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 39.7 bits (93), Expect = 3e-05
Identities = 30/105 (28%), Positives = 43/105 (40%), Gaps = 17/105 (16%)

Query: 22 AVSRGDAVADYIIDNVSILDLINAGEISGPIVIKGRYIAGVG-AEYADT---------PA 71
V+R D +I N ILD + G + I +K IA +G A D P
Sbjct: 60 QVTREGGAVDTVITNALILD--HWGIVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPG 117

Query: 72 LQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI 116
+ I G G +D+H+H + P E A L GLT ++
Sbjct: 118 TEVIAGEGKIVTAGGMDSHIH----FICPQQIEEA-LMSGLTCML 157


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20825TCRTETB340.001 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.1 bits (78), Expect = 0.001
Identities = 28/168 (16%), Positives = 61/168 (36%), Gaps = 17/168 (10%)

Query: 49 FNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFMLILSAIC 108
N++ D+ + + + F +T+ +G + +D K+ L F +I++ C
Sbjct: 33 LNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIIN--C 90

Query: 109 MLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTI----TKWTPRRKRGTFLGFW 164
+G SL +M + F Q G + + + ++ P+ RG G
Sbjct: 91 FGSVIGFVGHSFFSLLIM------ARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLI 144

Query: 165 NISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRY 212
+G + A+Y+ + + + P + I+ L
Sbjct: 145 GSIVAMGEGVGPAIGGMIAHYIHWSY---LLLIP--MITIITVPFLMK 187


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20830TCRTETB418e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 40.6 bits (95), Expect = 8e-06
Identities = 65/408 (15%), Positives = 137/408 (33%), Gaps = 60/408 (14%)

Query: 29 RHILLTIWLGYALFY--FTRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSG 86
RH + IWL F+ N ++P+I + + + T F +T+ + V G
Sbjct: 11 RHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYG 70

Query: 87 IVSDRSNARYFMGIGLIATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGS---PVCARLL 143
+SD+ + + G+I +++ S F L ++ F QG G+ P ++
Sbjct: 71 KLSDQLGIKRLLLFGIIINCFGSVIGFVGHS---FFSLLIMARFIQGAGAAAFPALVMVV 127

Query: 144 TAWY-SRTERGGWWALWNTAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLC 202
A Y + RG + L + +G + P + A + W ++ M ++ +
Sbjct: 128 VARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHW--SYLLLIPMITIITVPF- 184

Query: 203 WRLRDRPQALGLPAVGEWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLV 262
+ L +I G L I+ + Y VL
Sbjct: 185 --------LMKLLKKEVRIKGHFDIK----GIILMSVGIVFFMLFTTSYSISFLIVSVLS 232

Query: 263 YVV-----RAAINDWGNLYMSETLGVDLVTANTAVTMFELGGFI-----------GALVA 306
+++ R + + + + + + + + + GF+ A
Sbjct: 233 FLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA 292

Query: 307 GWGSDKLFNGNRGPMNLIFAAGILL-SVGSLWLMPFASYVMQATCFFTIGFFVFGPQMLI 365
GS +F G + + GIL+ G L+++ + + F T F + +
Sbjct: 293 EIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFL-SVSFLTASFLLETTSWFM 351

Query: 366 ---------GMAAAECS---------HKEAAGAATGFVGLFAYLGASL 395
G++ + ++ AGA + ++L
Sbjct: 352 TIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT 399


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20835PF06580402e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 39.8 bits (93), Expect = 2e-05
Identities = 28/142 (19%), Positives = 56/142 (39%), Gaps = 11/142 (7%)

Query: 365 LRPRQLDDLTLEQAIRSLMREMELEGRGIVSHLEWRIDESALSENQRVTLFRVCQEGLNN 424
LR ++L + + ++L L++ + + +V + Q + N
Sbjct: 208 LRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPM-LVQTLVEN 266

Query: 425 IVKHA-----DASAVTLQGWQQDERLMLVIEDDGSGLPPGSGQ-QGFGLTGMRERVTALG 478
+KH + L+G + + + L +E+ GS + + G GL +RER+ L
Sbjct: 267 GIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGTGLQNVRERLQMLY 326

Query: 479 G---TLHISCLHG-TRVSVSLP 496
G + +S G V +P
Sbjct: 327 GTEAQIKLSEKQGKVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_20840HTHFIS612e-13 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 61.4 bits (149), Expect = 2e-13
Identities = 29/174 (16%), Positives = 59/174 (33%), Gaps = 20/174 (11%)

Query: 2 ITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLPGRGVQVCICDISMPDIS 61
T+ + DD +R+ Q L V + + + + D+ MPD +
Sbjct: 4 ATILVADDDAAIRTVLNQALSRA-GYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDEN 61

Query: 62 GLELLSQLPK---GMATIMLSVHDSPALVEQALNAGARGFLSKRCSPDELIAAVHTVATG 118
+LL ++ K + +++S ++ +A GA +L K ELI +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR---- 117

Query: 119 GCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQG---MAVKEIAAELGLSPKTV 169
A+ R L + + + + + A L + T+
Sbjct: 118 --------ALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTL 163


96B7485_22160B7485_22195N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_22160-1131.645073MFS transporter
B7485_22165-1121.334400MFS transporter
B7485_22170-1131.644546DNA-binding transcriptional regulator OxyR
B7485_22175-1151.458475NAD(P)(+) transhydrogenase (Si-specific)
B7485_22180-1171.823139TetR family transcriptional regulator
B7485_22185-1182.557493hypothetical protein
B7485_22190-1193.114148tRNA (uridine(54)-C5)-methyltransferase TrmA
B7485_221950182.315575TonB-dependent vitamin B12 receptor
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22530TCRTETA332e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 33.3 bits (76), Expect = 2e-04
Identities = 17/63 (26%), Positives = 26/63 (41%)

Query: 57 ATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQALTGMVNSFSHFIL 116
+G+LL+ ++L G L DRFG R VL L ++ A+ +
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYI 101

Query: 117 MRI 119
RI
Sbjct: 102 GRI 104


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22540TCRTETB310.005 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.4 bits (71), Expect = 0.005
Identities = 45/302 (14%), Positives = 105/302 (34%), Gaps = 40/302 (13%)

Query: 7 LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP--ALVLMQLAWGWRT 64
G G A F + + + ++ RG A G+ S +G+ + P ++ + W +
Sbjct: 113 QGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLL 172

Query: 65 MFVIIGVAGILVGICWYAWYRNRAQ----------------FVL--TDEERTYLSASVKP 106
+ +I + + + F+L T ++L SV
Sbjct: 173 LIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLS 232

Query: 107 -----RPQLQFSEWL---ALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLA 158
+ + ++ L K+ +L + T +++ +P ++ S A
Sbjct: 233 FLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTA 292

Query: 159 KTGWVAAIP-FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSA--LGTLLVV 215
+ G V P ++ + ++ GI+VDR + +S L ++
Sbjct: 293 EIGSVIIFPGTMSVIIFGYIGGILVDRRG--------PLYVLNIGVTFLSVSFLTASFLL 344

Query: 216 QSSSPAQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIV 275
+++S + + + + F T +V + + + + + NF SF+ +
Sbjct: 345 ETTSWFMTIIIVFVLGG-LSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAI 403

Query: 276 TG 277
G
Sbjct: 404 VG 405


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22555HTHTETR506e-10 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 50.4 bits (120), Expect = 6e-10
Identities = 29/142 (20%), Positives = 62/142 (43%), Gaps = 3/142 (2%)

Query: 1 MGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGIAPTSFYRHFRDVDELGL 60
Q+ ++TR+ +++ A +L +++ +S SL E+A+ AG+ + Y HF+D +L
Sbjct: 2 ARKTKQEAQETRQHILDVA-LRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 TMVDESGLMLRQLMRQ-ARQRIAKGGSVIRTSVSTFMEFIGNNPNAFRLL-LRERSGTSA 118
+ + S + +L + + SV+R + +E L+ +
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 119 AFRAAVAREIQHFIAELADYLE 140
A V + ++ E D +E
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIE 142


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22570ACRIFLAVINRP300.043 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 29.8 bits (67), Expect = 0.043
Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 10/63 (15%)

Query: 22 DTSPDTLVVTAIRFEQPRSTVLAPTTVVTRQDIDRWQSTSVNDVLRRLPGV-DITQNGGS 80
+S L+V + P +T + DI + +++V D L RL GV D+ G
Sbjct: 131 KSSSSYLMVAGFVSDNPGTT---------QDDISDYVASNVKDTLSRLNGVGDVQLFGAQ 181

Query: 81 GQL 83
+
Sbjct: 182 YAM 184


97B7485_22465B7485_22505N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_22465-1150.767645NAD(P)-dependent oxidoreductase
B7485_22470-1181.802783Cro/Cl family transcriptional regulator
B7485_22475-2151.68832323S rRNA pseudouridine synthase F
B7485_22480-2151.541039hypothetical protein
B7485_22485-2151.565100IS3 family transposase
B7485_22490-2172.041681hypothetical protein
B7485_22495-2142.754179sensor histidine kinase
B7485_22505-2141.310077two-component system response regulator DcuR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22900DHBDHDRGNASE1155e-33 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 115 bits (289), Expect = 5e-33
Identities = 79/272 (29%), Positives = 128/272 (47%), Gaps = 27/272 (9%)

Query: 7 LQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHG-------GDGQYEGHKGYQFWPT 59
++ KI +TG A GIG A+ L +QGA++ VD + + E F P
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF-PA 64

Query: 60 DISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAPAGQYELNEAAFEKMVNI 119
D+ + ++ A I + G ID LVN AGV P L+ + L++ +E ++
Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLI---------HSLSDEEWEATFSV 115

Query: 120 NQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKEL 179
N GVF S++V++ M+ + G IV V S + YA++KAA FT+ EL
Sbjct: 116 NSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLEL 175

Query: 180 GKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQLREGYT---KNAIPIGRAGRL 236
++ IR ++PG E + W EQ+ +G K IP+ + +
Sbjct: 176 AEYNIRCNIVSPGSTETDMQWS-------LWADENGAEQVIKGSLETFKTGIPLKKLAKP 228

Query: 237 AEVADFVCYLLSERASYITGVTTNIAGGKTRG 268
+++AD V +L+S +A +IT + GG T G
Sbjct: 229 SDIADAVLFLVSGQAGHITMHNLCVDGGATLG 260


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22905HTHFIS290.033 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 28.6 bits (64), Expect = 0.033
Identities = 6/21 (28%), Positives = 12/21 (57%)

Query: 24 QAQIARELGIYRTTISRLLKR 44
Q + A LG+ R T+ + ++
Sbjct: 452 QIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22930PF06580418e-06 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 41.0 bits (96), Expect = 8e-06
Identities = 21/99 (21%), Positives = 38/99 (38%), Gaps = 18/99 (18%)

Query: 442 LIENALE-ALGP-EPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSE 499
L+EN ++ + GG+I + +G + EV + G +
Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKN------------TKES 310

Query: 500 RGVGLALVKQQVENLGG---SIAVESEPGIFTQFFVQIP 535
G GL V+++++ L G I + + G V IP
Sbjct: 311 TGTGLQNVRERLQMLYGTEAQIKLSEKQGKVN-AMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_22935HTHFIS712e-16 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 71.4 bits (175), Expect = 2e-16
Identities = 31/109 (28%), Positives = 50/109 (45%), Gaps = 4/109 (3%)

Query: 4 VLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDAPIDLILLDIYMQKEN 63
+L+ DDDA + + + +++ G+ S I + DL++ D+ M EN
Sbjct: 6 ILVADDDAAIRTVLNQALSRA-GYDVR-ITSNAATLWRWI--AAGDGDLVVTDVVMPDEN 61

Query: 64 GLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASR 112
DLLP + AR V+V+S+ T + G DYL KPF +
Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTE 110


98B7485_23000B7485_23025N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_23000-216-0.035147maltose/maltodextrin import ATP-binding protein
B7485_23005-216-0.961954sugar ABC transporter
B7485_23010-215-0.800763maltose ABC transporter substrate-binding
B7485_230150170.715288maltose ABC transporter permease MalF
B7485_230201180.467254maltose ABC transporter permease
B7485_230253291.513103D-xylose transporter XylE
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23355PF05272356e-04 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 34.7 bits (79), Expect = 6e-04
Identities = 13/35 (37%), Positives = 18/35 (51%)

Query: 32 VVFVGPSGCGKSTLLRMIAGLETITSGDLFIGEKR 66
VV G G GKSTL+ + GL+ + IG +
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGK 633


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23365MALTOSEBP7550.0 Maltose binding protein signature.
		>MALTOSEBP#Maltose binding protein signature.

Length = 396

Score = 755 bits (1951), Expect = 0.0
Identities = 395/396 (99%), Positives = 395/396 (99%)

Query: 1 MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK 60
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK
Sbjct: 1 MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK 60

Query: 61 VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW 120
VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW
Sbjct: 61 VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW 120

Query: 121 DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP 180
DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP
Sbjct: 121 DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP 180

Query: 181 YFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE 240
YFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE
Sbjct: 181 YFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE 240

Query: 241 AAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSTGINAASPNKE 300
AAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLS GINAASPNKE
Sbjct: 241 AAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE 300

Query: 301 LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP 360
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP
Sbjct: 301 LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP 360

Query: 361 QMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK 396
QMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK
Sbjct: 361 QMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK 396


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23370FLGHOOKAP1310.011 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 31.1 bits (70), Expect = 0.011
Identities = 22/124 (17%), Positives = 43/124 (34%), Gaps = 21/124 (16%)

Query: 128 GDEWQLALSDGETGKNYLSDAFKFGGEQKLQLKETTAQPEGERANLRVITQNRQALSDIT 187
++WQ+ T DA L+L T + L+ + A+ ++
Sbjct: 367 NNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPV---SDAIVNMD 423

Query: 188 AILPDGNKVMMSSLRQFSGTQPLYTLDGDGTLTNNQSGVKYRPNNQ--------IGFYQS 239
++ D K+ M+S GD N Q+ + + N++ Y S
Sbjct: 424 VLITDEAKIAMAS----------EEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYAS 473

Query: 240 ITAD 243
+ +D
Sbjct: 474 LVSD 477


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23380TCRTETA363e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 36.0 bits (83), Expect = 3e-04
Identities = 20/87 (22%), Positives = 42/87 (48%), Gaps = 3/87 (3%)

Query: 279 VIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMT--- 335
+I ++ ++ VGI +++ P + + L S D+ I++ + L A +
Sbjct: 7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGAL 66

Query: 336 VDKFGRKPLQIIGALGMAIGMFSLGTA 362
D+FGR+P+ ++ G A+ + TA
Sbjct: 67 SDRFGRRPVLLVSLAGAAVDYAIMATA 93


99B7485_23195B7485_23215N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_23195-2143.385239gluconate permease
B7485_23200-1163.302728fimbrial protein
B7485_232050152.732500fimbrial protein
B7485_232100132.256929type 1 fimbrial protein
B7485_23215-1151.396072fimbrial biogenesis outer membrane usher
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23535PF06580310.008 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 31.4 bits (71), Expect = 0.008
Identities = 10/49 (20%), Positives = 25/49 (51%)

Query: 230 LVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVAFV 278
+ +I ++ I +W V +T W ++ FI + P+A + + ++ +
Sbjct: 73 MGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPLALSII 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23540SURFACELAYER280.047 Lactobacillus surface layer protein signature.
		>SURFACELAYER#Lactobacillus surface layer protein signature.

Length = 439

Score = 28.1 bits (62), Expect = 0.047
Identities = 19/79 (24%), Positives = 32/79 (40%), Gaps = 1/79 (1%)

Query: 211 SQNLGYYLSGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVS 270
S+N G ++ +A+ N FT PA V V L ++G ++ + + +
Sbjct: 133 SENAGKEITIGSAN-PNVTFTEKTGDQPASTVKVTLDQDGVAKLSSVQIKNVYAIDTTYN 191

Query: 271 LGLTANYARTGGQVTAGNV 289
+ TG VT G V
Sbjct: 192 SNVNFYDVTTGATVTTGAV 210


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23545VACCYTOTOXIN334e-04 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 33.5 bits (76), Expect = 4e-04
Identities = 30/158 (18%), Positives = 49/158 (31%), Gaps = 9/158 (5%)

Query: 3 WCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAG 62
W R + A LA + +TI + VT VN + + + + G
Sbjct: 258 WMGRLQYVGAYLAPSYSTINTSKVTGEVNFNHLTVGDHNAAQAGIIASNKTH------IG 311

Query: 63 AASAWHDVALELTNCPVG--TSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLN 120
W L + P G + S + Q ++QN + N+
Sbjct: 312 TLDLWQSAGLNIIAPPEGGYKDKPNDKPSNTTQNNAKNDKQESSQNNSNTQVINPPNSAQ 371

Query: 121 TGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQA 158
+ QV D + V +N A GTI+
Sbjct: 372 KTEIQPTQVIDGPFAGGKNTVVNINRINTNA-DGTIRV 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_23555PF0057710860.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 1086 bits (2810), Expect = 0.0
Identities = 866/878 (98%), Positives = 871/878 (99%)

Query: 1 MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSSADLYFNLRFLADDPQA 60
MSYLNLRLYQRNTQCLHIRKHRLAGFFVRL VACAFAAQAPLSSA+LYFN RFLADDPQA
Sbjct: 1 MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSSAELYFNPRFLADDPQA 60

Query: 61 VADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLN 120
VADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLN
Sbjct: 61 VADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLN 120

Query: 121 TASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTIPQAFMSNRARGYIPPELWDP 180
TASV+GMNLLADDACVPLT+M+ DATA LDVGQQRLNLTIPQAFMSNRARGYIPPELWDP
Sbjct: 121 TASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDP 180

Query: 181 GINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSK 240
GINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSK
Sbjct: 181 GINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSK 240

Query: 241 NKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV 300
NKWQHI TW+ERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
Sbjct: 241 NKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV 300

Query: 301 IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTV 360
IHGIARGTAQVTIKQNGY IYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTV
Sbjct: 301 IHGIARGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTV 360

Query: 361 PYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY 420
PYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY
Sbjct: 361 PYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRY 420

Query: 421 RAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYR 480
RAFNFGIGKNM ALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYR
Sbjct: 421 RAFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYR 480

Query: 481 YSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRT 540
YSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRT
Sbjct: 481 YSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRT 540

Query: 541 STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600
STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI
Sbjct: 541 STLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI 600

Query: 601 PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD 660
PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD
Sbjct: 601 PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGD 660

Query: 661 GNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVL 720
GNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVL
Sbjct: 661 GNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVL 720

Query: 721 VKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVP 780
VKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVP
Sbjct: 721 VKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVP 780

Query: 781 TRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA 840
TRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA
Sbjct: 781 TRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA 840

Query: 841 GKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR 878
GKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR
Sbjct: 841 GKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR 878


100B7485_24385B7485_24415N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
B7485_24385222-2.819895phosphoglycerate mutase GpmB
B7485_24390221-2.708654right origin-binding protein
B7485_24395123-5.695891protein CreA
B7485_24400016-4.271921DNA-binding response regulator
B7485_24405013-4.203570two-component sensor histidine kinase
B7485_24410-112-4.023555cell envelope integrity protein CreD
B7485_24415-114-5.390384two-component system response regulator ArcA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24750VACCYTOTOXIN290.014 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 29.2 bits (65), Expect = 0.014
Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 4/45 (8%)

Query: 145 PLLVSHGIALGCLVSTILGLPAWAERRLRLRNCSISRVDYQESLW 189
P +V GIA G V T+ GL W ++ N D + +W
Sbjct: 42 PAIVG-GIATGAAVGTVSGLLGWGLKQAEEAN---KTPDKPDKVW 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24765HTHFIS876e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.8 bits (215), Expect = 6e-22
Identities = 33/139 (23%), Positives = 60/139 (43%)

Query: 1 MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKARQQVPDVMILDVGLPDI 60
M T+ + +D+ I L L + G+ V + + D+++ DV +PD
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 SGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYVAKPFSPREVCARVRTLLR 120
+ F+L ++ P LPVL ++A++ + + E GA DY+ KPF E+ + L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 RVKKFSTPSPVIRIGHFEL 139
K+ + L
Sbjct: 121 EPKRRPSKLEDDSQDGMPL 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24770PF06580330.003 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 32.9 bits (75), Expect = 0.003
Identities = 47/207 (22%), Positives = 80/207 (38%), Gaps = 51/207 (24%)

Query: 298 LTQNARMQAL---------VETL--LRQARLENRQEVVLTAVDVAALFR---RVSEARTV 343
+ Q A++ AL L +R LE+ + ++ L R R S AR V
Sbjct: 157 MAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKAREMLTSLSELMRYSLRYSNARQV 216

Query: 344 QLAE--KNITLHVM--------PTEVNVAAEPALLDQALGNLL-----DNA----IDFTP 384
LA+ + ++ + PA++D + +L +N I P
Sbjct: 217 SLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQVPPMLVQTLVENGIKHGIAQLP 276

Query: 385 ESGCITLSAEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSE 444
+ G I L D VTL+V +TGS N ++S+G GL V E
Sbjct: 277 QGGKILLKGTKDNGTVTLEVENTGSLALK----------------NTKESTGTGLQNVRE 320

Query: 445 -VARLFNGEVTLR-NVQEGGVLASLRL 469
+ L+ E ++ + ++G V A + +
Sbjct: 321 RLQMLYGTEAQIKLSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
B7485_24780HTHFIS824e-20 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 81.8 bits (202), Expect = 4e-20
Identities = 30/122 (24%), Positives = 60/122 (49%), Gaps = 1/122 (0%)

Query: 1 MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILSEYDINLVIMDINLPGK 60
M IL+ +D+ R L GYDV ++ A + + ++ D +LV+ D+ +P +
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 NGLLLARELRE-QANVALMFLTGRDNEVDKILGLEIGADDYITKPFNPRELTIRARNLLS 119
N L +++ + ++ ++ ++ ++ + I E GA DY+ KPF+ EL L+
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 120 RT 121

Sbjct: 121 EP 122



 
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