PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genome483.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_009076 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1BURPS1106A_0012BURPS1106A_0022Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_00120133.210472general secretion pathway protein G
BURPS1106A_0013-1143.887464general secretion pathway protein H
BURPS1106A_00140133.602457general secretion pathway protein I
BURPS1106A_0015-2114.122225general secretion pathway protein J
BURPS1106A_0016-2104.245445general secretion pathway protein K
BURPS1106A_0017-1104.230768general secretion pathway protein L
BURPS1106A_0018-1112.220365general secretory pathway protein M
BURPS1106A_00190112.286520general secretory pathway protein N
BURPS1106A_00200122.746208NodT family efflux transporter outer membrane
BURPS1106A_00212141.356525hypothetical protein
BURPS1106A_00222151.695648MarR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0012BCTERIALGSPG1886e-65 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 188 bits (480), Expect = 6e-65
Identities = 67/140 (47%), Positives = 94/140 (67%), Gaps = 3/140 (2%)

Query: 10 QAARRQRGFTLIEIMVVVAILGILAALIVPKIMSRPDEARRIAAKQDIGTIMQALKLYRL 69
+A +QRGFTL+EIMVV+ I+G+LA+L+VP +M ++A + A DI + AL +Y+L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKL 61

Query: 70 DNGRYPTQDQGLNALIQKPTTDPIPNNWKDGGYLERLPNDPWGNSYKYLNPGVHGEIDVF 129
DN YPT +QGL +L++ PT P+ N+ GY++RLP DPWGN Y +NPG HG D+
Sbjct: 62 DNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLL 121

Query: 130 SYGADGKEGGESNDSDIGSW 149
S G DG+ G E DI +W
Sbjct: 122 SAGPDGEMGTE---DDITNW 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0013BCTERIALGSPH521e-10 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 51.9 bits (124), Expect = 1e-10
Identities = 20/101 (19%), Positives = 33/101 (32%), Gaps = 15/101 (14%)

Query: 51 RARGFTLLEMLVVLVIAGILVSVASLTLRRNPRTDLREEAQRIALLFETAGDEAQVRARP 110
R RGFTLLEM+++L++ G+ + L + + R +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQF 61

Query: 111 IAWRATEHGFRF---------------DIRTGDGWRPLRDD 136
++F D +G W PLR
Sbjct: 62 FGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAG 102


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0014BCTERIALGSPG300.001 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 30.2 bits (68), Expect = 0.001
Identities = 10/26 (38%), Positives = 18/26 (69%)

Query: 10 RSPARSRGFTMIEVLVALAIIAVALA 35
R+ + RGFT++E++V + II V +
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLAS 27


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0015BCTERIALGSPG333e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 33.3 bits (76), Expect = 3e-04
Identities = 17/72 (23%), Positives = 34/72 (47%), Gaps = 3/72 (4%)

Query: 33 RGFTLIEMMIAITILAVIA-ILSWRGLDQIIRGREKVAAAMEDERVFAQMFDQMRIDARR 91
RGFTL+E+M+ I I+ V+A ++ + + ++ A+ D D ++D
Sbjct: 8 RGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK--AVSDIVALENALDMYKLDNHH 65

Query: 92 AATDDEAGQPAV 103
T ++ + V
Sbjct: 66 YPTTNQGLESLV 77


2BURPS1106A_0070BURPS1106A_0126Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0070-1123.328943hypothetical protein
BURPS1106A_0071-1112.653207hypothetical protein
BURPS1106A_0072-1112.364602acetyl-CoA acetyltransferase
BURPS1106A_0073-2132.0125653-hydroxyacyl-CoA dehydrogenase
BURPS1106A_00744170.886465hypothetical protein
BURPS1106A_00753150.732592CAIB/BAIF family protein
BURPS1106A_0076624-1.114100lipoprotein
BURPS1106A_0077828-0.474349hypothetical protein
BURPS1106A_00785211.411432hypothetical protein
BURPS1106A_00793191.447034hypothetical protein
BURPS1106A_00800152.437221lipoprotein
BURPS1106A_00823231.702437hypothetical protein
BURPS1106A_0081331-1.999628hypothetical protein
BURPS1106A_0083230-2.975392transmembrane regulator PrtR
BURPS1106A_0084335-5.721410sigma-70 family RNA polymerase sigma factor
BURPS1106A_0085641-6.771536catalase
BURPS1106A_00861152-11.683116[Ni] hydrogenase, b-type cytochrome subunit
BURPS1106A_00871049-11.421373methionine sulfoxide reductase A
BURPS1106A_0088737-10.022778hypothetical protein
BURPS1106A_0089440-9.096032transposase subfamily protein
BURPS1106A_0090440-8.894819transposase
BURPS1106A_0091339-9.111122transposase A
BURPS1106A_0092537-9.309224transposase B
BURPS1106A_0093641-10.621338hypothetical protein
BURPS1106A_0094740-9.671207hypothetical protein
BURPS1106A_0095839-9.873872XRE family transcriptional regulator
BURPS1106A_0096528-8.579227hypothetical protein
BURPS1106A_0097524-8.102236type I restriction-modification system M
BURPS1106A_0098319-6.775113restriction endonuclease S subunits
BURPS1106A_0099214-5.824514type I site-specific deoxyribonuclease HsdR
BURPS1106A_0100010-4.015188hypothetical protein
BURPS1106A_0101-112-3.500367DNA gyrase subunit B
BURPS1106A_0102012-3.400467DNA polymerase III subunit beta
BURPS1106A_0103012-3.424916chromosomal replication initiation protein
BURPS1106A_0104217-4.56732750S ribosomal protein L34
BURPS1106A_0105219-5.470845ribonuclease P protein component
BURPS1106A_0106-117-3.903624hypothetical protein
BURPS1106A_0107-118-5.015694inner membrane protein translocase component
BURPS1106A_0108021-3.605848hypothetical protein
BURPS1106A_0109020-3.419514hypothetical protein
BURPS1106A_0110-119-3.205953hypothetical protein
BURPS1106A_0111-119-2.652608tRNA modification GTPase TrmE
BURPS1106A_0112-119-3.372469phage integrase family site specific
BURPS1106A_0113-116-1.934564hypothetical protein
BURPS1106A_0114330-5.733460hypothetical protein
BURPS1106A_0115235-6.572073hypothetical protein
BURPS1106A_0116435-6.589310hypothetical protein
BURPS1106A_0117635-7.419879hypothetical protein
BURPS1106A_0118541-9.070343hypothetical protein
BURPS1106A_0119430-7.511929hypothetical protein
BURPS1106A_0120327-6.796588hypothetical protein
BURPS1106A_0121324-7.190929TnpB
BURPS1106A_0122225-7.779118hypothetical protein
BURPS1106A_0123224-7.547482hypothetical protein
BURPS1106A_0124318-5.668901lipoprotein
BURPS1106A_0125023-6.207079hypothetical protein
BURPS1106A_0126124-5.088711diamine N-acetyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0075SHAPEPROTEIN320.004 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 32.0 bits (73), Expect = 0.004
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 3/67 (4%)

Query: 144 AGQPGDAPFAPPTLVGDLGGGALYLAMGVLAGIVDAR-LRGKGQIVDAAIVDGSANLMNL 202
AG P ++V D+GGG +A+ L G+V + +R G D AI++
Sbjct: 151 AGLPVSEATG--SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGS 208

Query: 203 LLSIHAA 209
L+ A
Sbjct: 209 LIGEATA 215


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0079UREASE300.014 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 30.1 bits (68), Expect = 0.014
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 5/44 (11%)

Query: 282 GGILVYDQFVTP----PTPQPVRQRRLRWGAHGRSNNGDNFYVV 321
GG + P PTPQPV R + +GA+GRS + V
Sbjct: 452 GGTIAAAPMGDPNASIPTPQPVHYRPM-FGAYGRSRTNSSVTFV 494


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0087BCTERIALGSPG280.033 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 27.9 bits (62), Expect = 0.033
Identities = 12/32 (37%), Positives = 17/32 (53%)

Query: 102 NLTKAHIKYLESSLVALSKNADRYQLENGNTP 133
N KA + S +VAL D Y+L+N + P
Sbjct: 36 NKEKADKQKAVSDIVALENALDMYKLDNHHYP 67


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0103PERTACTIN330.004 Pertactin signature.
		>PERTACTIN#Pertactin signature.

Length = 922

Score = 32.8 bits (74), Expect = 0.004
Identities = 24/93 (25%), Positives = 31/93 (33%)

Query: 81 PKAGQRSPAGATPLAPRAPLPSANPAPVAPGPACAPAVDAHAPAPAGMNAATAAAVAAAQ 140
P A + +P P+ P P P P P +A AP P +AAA AA
Sbjct: 568 PPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAPQPPAGRELSAAANAAVN 627

Query: 141 AAQAAQANAAALNADEAADLDLPSLTAHEAAAG 173
A+ A L L + A G
Sbjct: 628 TGGVGLASTLWYAESNALSKRLGELRLNPDAGG 660


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_010760KDINNERMP490e-171 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 490 bits (1263), Expect = e-171
Identities = 204/576 (35%), Positives = 320/576 (55%), Gaps = 46/576 (7%)

Query: 1 MDIKRTVLWVIFFMSAVMLFDNWQRSHGRPSMFFPNVTQTNTASNATNGNGASGASAAAA 60
MD +R +L + + M++ W++ Q T + T
Sbjct: 1 MDSQRNLLVIALLFVSFMIWQAWEQDKNPQ-----PQAQQTTQTTTT------------- 42

Query: 61 ANALPAAATGAAPATTAPAAQAQLVRFSTDVYNGEIDTRGGTLAKLTLTK---AGDGKQP 117
AA AA + Q +L+ TDV + I+TRGG + + L + QP
Sbjct: 43 ------AAGSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQP 96

Query: 118 DLSVTLFDHTANHTYLARTGLLGGDFPN-----HNDVYAQVAGPTSLAADQNTLKLSFES 172
L + + Y A++GL G D P+ +Y LA QN L++
Sbjct: 97 ---FQLLETSPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTY 153

Query: 173 PVKGGVKVVKTYTFTRGSYVIGVDTKIENVGAAPVTPSVYMELVRD-----NSSVETPMF 227
G KT+ RG Y + V+ ++N G P+ S + +L + + + F
Sbjct: 154 TDAAGNTFTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNF 213

Query: 228 S-HTFLGPAVYTDQKHFQKITFGDIDKNKADYVTSADNGWIAMVQHYFASAWIPQSGAKR 286
+ HTF G A T + ++K F I N+ ++S GW+AM+Q YFA+AWIP +
Sbjct: 214 ALHTFRGAAYSTPDEKYEKYKFDTIADNENLNISS-KGGWVAMLQQYFATAWIPHNDGTN 272

Query: 287 DIYVEKIDPTLYRVGVKQPVAAIAPGQSADVSARLFAGPEEERMLEGIAPGLELVKDYGW 346
+ Y + + +G K + PGQ+ +++ L+ GPE + + +AP L+L DYGW
Sbjct: 273 NFYTANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGW 332

Query: 347 VTIIAKPLFWLLEKIHGFVGNWGWAIVLLTLLIKAVFFPLSAASYKSMARMKEITPRMQA 406
+ I++PLF LL+ IH FVGNWG++I+++T +++ + +PL+ A Y SMA+M+ + P++QA
Sbjct: 333 LWFISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA 392

Query: 407 LRERFKSDPQKMNAALMELYKTEKVNPFGGCLPVVIQIPVFISLYWVLLASVEMRGAPWV 466
+RER D Q+++ +M LYK EKVNP GGC P++IQ+P+F++LY++L+ SVE+R AP+
Sbjct: 393 MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAPFA 452

Query: 467 LWIHDLSQRDPYFILPVLMAVSMFVQTKLNPTP-PDPVQAKMMMFMPIAFSVMFFFFPAG 525
LWIHDLS +DPY+ILP+LM V+MF K++PT DP+Q K+M FMP+ F+V F +FP+G
Sbjct: 453 LWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSG 512

Query: 526 LVLYYVVNNVLSIAQQYYITRTL---GGAAAKKKAS 558
LVLYY+V+N+++I QQ I R L G + +KK S
Sbjct: 513 LVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS 548


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0111PF05272372e-04 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 36.6 bits (84), Expect = 2e-04
Identities = 25/123 (20%), Positives = 40/123 (32%), Gaps = 9/123 (7%)

Query: 191 IDFLEAADARGKLAHIR--ERLAHVLGDARQGALLREGLSV----VLAGQPNVGKSSLLN 244
+ L K +R + + + ++ G VL G +GKS+L+N
Sbjct: 555 VHVLGKTPDDYKPRRLRYLQLVGKYILMGHVARVMEPGCKFDYSVVLEGTGGIGKSTLIN 614

Query: 245 ALAGAELAIVTPI-AGTTRDKVAQTIQIEGIPLHIIDTAGLRETEDEVEKIGIARTWGEI 303
L G + T GT +D Q I L + R + E K +
Sbjct: 615 TLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSEMT--AFRRADAEAVKAFFSSRKDRY 672

Query: 304 ERA 306
A
Sbjct: 673 RGA 675


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0126SACTRNSFRASE418e-07 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 40.7 bits (95), Expect = 8e-07
Identities = 23/113 (20%), Positives = 45/113 (39%), Gaps = 11/113 (9%)

Query: 37 FEEPYETFTELSQLYDQHVHDQRERRFVAFDSDGELVGLVELI----ELDYIHRRGEFQI 92
F +PY E + +V ++ + F+ + + +G +++ I I
Sbjct: 42 FSKPYFKQYEDDDMDVSYVEEEGKAAFLYY-LENNCIGRIKIRSNWNGYALIE-----DI 95

Query: 93 IIAPNRQGRGFATRATRLAVEYAFKVLNLRKLYLIVDKSNVAAIRVYEKCGFK 145
+A + + +G T A+E+A K + L L N++A Y K F
Sbjct: 96 AVAKDYRKKGVGTALLHKAIEWA-KENHFCGLMLETQDINISACHFYAKHHFI 147


3BURPS1106A_0227BURPS1106A_0257Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0227-1113.688396acyl-CoA dehydrogenase
BURPS1106A_02281103.143758GMC family oxidoreductase
BURPS1106A_02291123.255666flagellar hook-length control protein FliK
BURPS1106A_02302141.707573flagellar export protein FliJ
BURPS1106A_02313121.340081flagellar protein export ATPase FliI
BURPS1106A_02321120.313184flagellar assembly protein H
BURPS1106A_02331112.406141flagellar motor switch protein G
BURPS1106A_02340103.790535flagellar MS-ring protein
BURPS1106A_02351104.651683flagellar hook-basal body complex protein FliE
BURPS1106A_0236194.860210flagellar protein FliS
BURPS1106A_0237-283.974993hypothetical protein
BURPS1106A_0238-193.308691hypothetical protein
BURPS1106A_0239-1122.159717flagellar biosynthesis protein
BURPS1106A_02401121.071806hypothetical protein
BURPS1106A_02410131.309733xanthine dehydrogenase accessory factor
BURPS1106A_02420141.392359amino acid permease
BURPS1106A_0243-2132.327437LuxR family DNA-binding response regulator
BURPS1106A_0244-3133.323708sensor histidine kinase
BURPS1106A_0245-4121.086085ferredoxin--NADP reductase
BURPS1106A_0246-1102.553107LysE family translocator protein
BURPS1106A_0247-1101.880516endonuclease/exonuclease/phosphatase family
BURPS1106A_0248-2100.764447hypothetical protein
BURPS1106A_0249-3120.129925hypothetical protein
BURPS1106A_0250-312-0.569168ATP-dependent protease domain-containing
BURPS1106A_0251-410-2.184323FAD-dependent oxidoreductase
BURPS1106A_0252-211-3.244151high-potential iron-sulfur protein
BURPS1106A_0253-111-2.359703major facilitator family transporter
BURPS1106A_0255111-2.587347hypothetical protein
BURPS1106A_0254112-1.979025hypothetical protein
BURPS1106A_0256-112-0.546040dipeptide ABC transporter periplasmic
BURPS1106A_02572120.862087dipeptide ABC transporter permease DppB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0229FLGHOOKFLIK733e-16 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 73.3 bits (179), Expect = 3e-16
Identities = 79/257 (30%), Positives = 109/257 (42%), Gaps = 8/257 (3%)

Query: 205 NGDASAPLAANRAAFDKLLAGAKAPAAQAAPTDASGANPATALANAAANAAQPDASG--A 262
N D +A L+A A K A + T L + AQPD +
Sbjct: 124 NEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKPTLFTKLTSEQLTTAQPDDAPGTP 183

Query: 263 LAALQDAADSARATLAASSAPAALQQAA-PAALAANASAAAASAAPSLAPPVGTPDWTDA 321
L A++ S P+ + AA P AAP L+ P+G+ +W +
Sbjct: 184 AQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPHQTQPLPTVAAPVLSAPLGSHEWQQS 243

Query: 322 LSQKVVFLSNAHQQSAELTLNPPDLGPLQVVLRVADNHAHALFVSQHAQVRDAVEAALPK 381
LSQ + + QQSAEL L+P DLG +Q+ L+V DN A VS H VR A+EAALP
Sbjct: 244 LSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPV 303

Query: 382 LREAMEAGGLGLGSASVSDGGFASAQQQQTPQRQSSDGSATRRAFGASTADAALDELAAA 441
LR + G+ LG +++S F+ QQ + Q+QS +A D L
Sbjct: 304 LRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQR-TANHEPLAGEDDDT----LPVP 358

Query: 442 SSGGAARRAVGMVDTFA 458
S VD FA
Sbjct: 359 VSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0230FLGFLIJ602e-14 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 59.8 bits (144), Expect = 2e-14
Identities = 43/140 (30%), Positives = 74/140 (52%)

Query: 1 MAQSFPLQLLLERAQDDLDTAAKQLGRAQRERTDAQAQLDALMRYRDEYRVRFAESAQSG 60
MA+ L L + A+ +++ AA+ LG +R A+ QL L+ Y++EYR +G
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 MPAGNWRNFQAFLDTLDAAIEQQRRVLAAAQTRIDAARPEWQAKKRTLGSYEILQARGAR 120
+ + W N+Q F+ TL+ AI Q R+ L ++D A W+ KK+ L +++ LQ R +
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 QDAQRAAKREQRDADEHAAK 140
+ +Q+ DE A +
Sbjct: 121 AALLAENRLDQKKMDEFAQR 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0232FLGFLIH1083e-31 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 108 bits (271), Expect = 3e-31
Identities = 64/184 (34%), Positives = 106/184 (57%), Gaps = 4/184 (2%)

Query: 37 AAAALAAELQRVRDAAHAEGLAAGHVEGQALGYQAGYEQGRAKGFDEGRAEAHTHAAQLA 96
A +L +L +++ AH +G AG EG+ G++ GY++G A+G ++G AEA + A +
Sbjct: 36 AEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIH 95

Query: 97 A----LAASFRDALAGVERDLADDIATLALEIAQQVVRQHVQHDPAALIAAAREVLAAEP 152
A L + F+ L ++ +A + +ALE A+QV+ Q D +ALI +++L EP
Sbjct: 96 ARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEP 155

Query: 153 ALAGAPHLIVNPADLPVVEAYLKDELDTLGWSVRTDTSIERGGCRAHASTGEIDATLTTR 212
+G P L V+P DL V+ L L GW +R D ++ GGC+ A G++DA++ TR
Sbjct: 156 LFSGKPQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATR 215

Query: 213 WERV 216
W+ +
Sbjct: 216 WQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0233FLGMOTORFLIG298e-102 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 298 bits (765), Expect = e-102
Identities = 114/324 (35%), Positives = 191/324 (58%)

Query: 5 GLNKSALLLMSIGEEEAAQVFKFLAPREVQKIGAAMAALKNVTREQVEDVLNDFVQEAEK 64
G K+A+LL+SIG E +++VFK+L+ E++ + +A L+ +T E ++VL +F +
Sbjct: 17 GKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEFKELMMA 76

Query: 65 HTALSLDSSEYIRTVLTKALGEDKAGVLIDRILQGSDTSGIEGLKWMDSAAVAELIKNEH 124
+ +Y R +L K+LG KA +I+ + + E ++ D A + I+ EH
Sbjct: 77 QEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFVRRADPANILNFIQQEH 136

Query: 125 PQIIATILVHLDRDQASEIASCFTERLRNDVLLRIATLDGIQPTALRELDDVLTGLLSGS 184
PQ IA IL +LD +AS I S ++ +V RIA +D P +RE++ VL L+
Sbjct: 137 PQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVERVLEKKLASL 196

Query: 185 DNLKRAPMGGIRTAAEILNFMTSVHEEAVIENVKQYDPDLAQKIIDQMFVFENLLDLEDR 244
+ GG+ EI+N E+ +IE++++ DP+LA++I +MFVFE+++ L+DR
Sbjct: 197 SSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMFVFEDIVLLDDR 256

Query: 245 AIQLLLKEVESEALIIALKGAPPALRQKFLSNMSQRAAELLAEDLDARGPVRVSEVETQQ 304
+IQ +L+E++ + L ALK +++K NMS+RAA +L ED++ GP R +VE Q
Sbjct: 257 SIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLGPTRRKDVEESQ 316

Query: 305 RKILQVVRNLAESGQIVIGGKAED 328
+KI+ ++R L E G+IVI E+
Sbjct: 317 QKIVSLIRKLEEQGEIVISRGGEE 340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0234FLGMRINGFLIF468e-162 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 468 bits (1206), Expect = e-162
Identities = 254/562 (45%), Positives = 360/562 (64%), Gaps = 37/562 (6%)

Query: 53 LSRMKTNPRLPFLIGAALAIAAIVALVLWSRAPDYRVLYSNLSDRDGGAIIAALQQANVP 112
L+R++ NPR+P ++ + A+A +VA+VLW++ PDYR L+SNLSD+DGGAI+A L Q N+P
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 113 YKFADAGGAILVPANQVHETRLKLAAMGLPKGGSVGFELMDNQKFGISQFAEQVNYQRAL 172
Y+FA+ GAI VPA++VHE RL+LA GLPKGG+VGFEL+D +KFGISQF+EQVNYQRAL
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRAL 135

Query: 173 EGELQRTVESINAVRAARVHLAIPKPSVFVRDREAPSASVLVDLYPGRVLDEGQVLAVTR 232
EGEL RT+E++ V++ARVHLA+PKPS+FVR++++PSASV V L PGR LDEGQ+ AV
Sbjct: 136 EGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVH 195

Query: 233 MVSSSVPDMPAKNVTIVDQDGNLLTQT-ASATGLDASQLKYVQQIERNTQKRIDAILAPI 291
+VSS+V +P NVT+VDQ G+LLTQ+ S L+ +QLK+ +E Q+RI+AIL+PI
Sbjct: 196 LVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDVESRIQRRIEAILSPI 255

Query: 292 FGAGNARSQVSADVDFSKIEQTSESYGPNGTPQQSAIRSQQTSSSTELAQSGASGVPGAL 351
G GN +QV+A +DF+ EQT E Y PNG ++ +RS+Q + S ++ GVPGAL
Sbjct: 256 VGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGAL 315

Query: 352 SNTPPQPASAPIVA-------------SNGQPAGPAATPVSDRKDSTTNYELDKTVRHVE 398
SN P P API ++ +A P S +++ T+NYE+D+T+RH +
Sbjct: 316 SNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHTK 375

Query: 399 QSMGTIKRLSVAVVVNYQPSTDAKGRVTMQPLAADKLAQVQQLVKDAMGYDEKRGDSVNV 458
++G I+RLSVAVVVNY+ D K PL AD++ Q++ L ++AMG+ +KRGD++NV
Sbjct: 376 MNVGDIERLSVAVVVNYKTLADGKP----LPLTADQMKQIEDLTREAMGFSDKRGDTLNV 431

Query: 459 VNSAFSAAADPFANLPWWRQPDMIELGKDIAKWLGVAAAAAALYFMFVRPALRR---AFP 515
VNS FSA + LP+W+Q I+ +WL V A L+ VRP L R
Sbjct: 432 VNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVRPQLTRRVEEAK 491

Query: 516 PPAEPAAAAVPALDGPDDMLALDGLPSPDKKQLAEEDEEHPALLAFENERNRYERNLDYA 575
E A + + L+ D + N+R E
Sbjct: 492 AAQEQAQVRQETEEAVEVRLSKDEQLQQRR----------------ANQRLGAEVMSQRI 535

Query: 576 RTIARQDPKIVATVVKNWVSDE 597
R ++ DP++VA V++ W+S++
Sbjct: 536 REMSDNDPRVVALVIRQWMSND 557


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0235FLGHOOKFLIE619e-16 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 61.2 bits (148), Expect = 9e-16
Identities = 47/111 (42%), Positives = 62/111 (55%), Gaps = 8/111 (7%)

Query: 3 APVNGIASALQQMQAMAAQAAGGASPATSLAGSGAASAGSFASAMKASLDKISGDQQKAL 62
+ + GI + Q+QA A A S SFA + A+LD+IS Q A
Sbjct: 1 SAIQGIEGVISQLQATAMSARAQES--------LPQPTISFAGQLHAALDRISDTQTAAR 52

Query: 63 GEAHAFEIGAQNVSLNDVMVDMQKANIGFQFGLQVRNKLVSAYNEIMQMSV 113
+A F +G V+LNDVM DMQKA++ Q G+QVRNKLV+AY E+M M V
Sbjct: 53 TQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0239TYPE3IMSPROT624e-15 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 62.5 bits (152), Expect = 4e-15
Identities = 17/81 (20%), Positives = 32/81 (39%), Gaps = 1/81 (1%)

Query: 10 AVLAYDAKGGDTAPRVVAKGYGLVAERIIERARDAGLYVHTAPEMV-SLLMQVDLDARIP 68
A+ +G P V K + + + A + G+ + + +L +D IP
Sbjct: 268 AIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDHYIP 327

Query: 69 PQLYQAVAELLAWLYALERDA 89
+ +A AE+L WL +
Sbjct: 328 AEQIEATAEVLRWLERQNIEK 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0243HTHFIS853e-21 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 84.9 bits (210), Expect = 3e-21
Identities = 31/114 (27%), Positives = 55/114 (48%), Gaps = 2/114 (1%)

Query: 5 ILLVDDHAIVRQGIRQLLIDRGIAREVKEAECGGDALVIAEKSEFDVILLDISLPDMNGI 64
IL+ DD A +R + Q L G +V+ + D+++ D+ +PD N
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGY--DVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 65 EVLKRLKRRLPSTPVLMFSMYREDQFAVRALKAGAAGYLSKTVNAAQMVSAISQ 118
++L R+K+ P PVL+ S A++A + GA YL K + +++ I +
Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0250HTHFIS347e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.0 bits (78), Expect = 7e-04
Identities = 21/103 (20%), Positives = 40/103 (38%), Gaps = 18/103 (17%)

Query: 24 IETALNDLNEGASDAL---------RATYEKMLKTGNLRFCVKPTRMPAFDSLAQALPNF 74
TA+ +GA D L + L R P+++ L
Sbjct: 87 FMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRR----PSKLEDDSQDGMPLVGR 142

Query: 75 AEPLDDVRKQVALCLETDDRLELMPILLLGEPGIGKTHFAKAL 117
+ + ++ + +A ++TD + +++ GE G GK A+AL
Sbjct: 143 SAAMQEIYRVLARLMQTD-----LTLMITGESGTGKELVARAL 180


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0253TCRTETA330.002 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 32.9 bits (75), Expect = 0.002
Identities = 22/107 (20%), Positives = 43/107 (40%), Gaps = 9/107 (8%)

Query: 70 FMRPIGGIVLGLYADRAGRKAALSLVILLMTFGIFLIAVAPPYAAIGIGGPLLIVLGRLL 129
M+ VLG +DR GR+ L + + ++A AP ++ +GR++
Sbjct: 54 LMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLW--------VLYIGRIV 105

Query: 130 QGFSAGGEFGSATALLIEAAPLSRRGYYGSWQMASQAAALLFGSLVG 176
G + G A A + + R + + A ++ G ++G
Sbjct: 106 AGIT-GATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG 151


4BURPS1106A_0268BURPS1106A_0291Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_02682112.9267445-formyltetrahydrofolate cyclo-ligase
BURPS1106A_02691102.257993Slt family transglycosylase
BURPS1106A_02700123.131008NAD-dependent epimerase/dehydratase family
BURPS1106A_0271-1132.503964glutathione S-transferase domain-containing
BURPS1106A_02720134.811890multifunctional tRNA nucleotidyl
BURPS1106A_02732164.332406hypothetical protein
BURPS1106A_02740133.449012RebB protein
BURPS1106A_0275-1122.902437FlgN family flagellar protein
BURPS1106A_02762121.427396flagellar biosynthesis regulator protein FlgM
BURPS1106A_02772111.167364flagellar basal body P-ring biosynthesis protein
BURPS1106A_0278517-1.938318flagellar basal body rod protein FlgB
BURPS1106A_0279418-1.860781flagellar basal body rod protein FlgC
BURPS1106A_0280420-0.822271flagellar basal body rod modification protein
BURPS1106A_0281020-0.870910flagellar hook protein FlgE
BURPS1106A_0282-118-0.123650flagellar basal body rod protein FlgF
BURPS1106A_0283117-0.166037flagellar basal body rod protein FlgG
BURPS1106A_02842170.088970flagellar basal body L-ring protein
BURPS1106A_02853150.280636flagellar basal body P-ring protein
BURPS1106A_0286112-0.045075flagellar rod assembly protein/muramidase FlgJ
BURPS1106A_02872110.372267hypothetical protein
BURPS1106A_02881110.742685flagellar hook-associated protein FlgK
BURPS1106A_02890131.543707flagellar hook-associated protein FlgL
BURPS1106A_02901122.117755hypothetical protein
BURPS1106A_02913122.117755uracil-xanthine permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0271cloacin300.009 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 29.7 bits (66), Expect = 0.009
Identities = 22/64 (34%), Positives = 32/64 (50%), Gaps = 11/64 (17%)

Query: 96 SVSAEMHAGFPALRSEMPLNVRESHPGRGATPAALADVARIDELWRTCVAASGGPFLFGA 155
+V+A + GFPAL + + S GA AA+AD+ +AA GPF FG
Sbjct: 83 AVAAPVAFGFPALSTPGAGGLAVSISA-GALSAAIADI----------MAALKGPFKFGL 131

Query: 156 FSIA 159
+ +A
Sbjct: 132 WGVA 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0279FLGHOOKAP1270.029 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 26.8 bits (59), Expect = 0.029
Identities = 10/38 (26%), Positives = 17/38 (44%)

Query: 102 NVDPVQEMVNMISASRSYQANVETLNTAKQLMLKTLTI 139
V+ +E N+ + Y AN + L TA + + I
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0281FLGHOOKAP1340.001 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 34.2 bits (78), Expect = 0.001
Identities = 17/58 (29%), Positives = 24/58 (41%)

Query: 356 ISAPGSTNHGTLQGSALENSNVDLTSQLVKLITAQRNYQANAQTIKTQQTVDQTLINL 413
SA L S V+L + L Q+ Y ANAQ ++T + LIN+
Sbjct: 488 SSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 30.3 bits (68), Expect = 0.017
Identities = 11/31 (35%), Positives = 17/31 (54%)

Query: 6 GLSGLAGASSDLDVIGNNIANANTVGFKGST 36
+SGL A + L+ NNI++ N G+ T
Sbjct: 7 AMSGLNAAQAALNTASNNISSYNVAGYTRQT 37


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0282FLGHOOKAP1290.018 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 29.2 bits (65), Expect = 0.018
Identities = 9/34 (26%), Positives = 18/34 (52%)

Query: 4 LIYTAMTGATQSLEQQSVVANNLANASTTGFRAQ 37
LI AM+G + + +NN+++ + G+ Q
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQ 36


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0283FLGHOOKAP1421e-06 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 42.3 bits (99), Expect = 1e-06
Identities = 10/48 (20%), Positives = 23/48 (47%)

Query: 213 TLKQGYVESSNVNVVQELVNMIQTQRAYEINSKAVTTSDQMLQTVTQM 260
L S VN+ +E N+ + Q+ Y N++ + T++ + + +
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 40.3 bits (94), Expect = 5e-06
Identities = 19/80 (23%), Positives = 34/80 (42%), Gaps = 14/80 (17%)

Query: 4 SLYIAATGMNAQQAQMDVISNNLANVSTNGFKGSRAVFEDLLYQTVRQPGANSTQQTELP 63
+ A +G+NA QA ++ SNN+++ + G+ RQ + + L
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYT--------------RQTTIMAQANSTLG 48

Query: 64 SGLQLGTGVQQVATERLYTQ 83
+G +G GV +R Y
Sbjct: 49 AGGWVGNGVYVSGVQREYDA 68


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0284FLGLRINGFLGH2063e-69 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 206 bits (526), Expect = 3e-69
Identities = 128/222 (57%), Positives = 156/222 (70%), Gaps = 7/222 (3%)

Query: 25 AALAAAALALAGCAQIPREPITQQPMSAMPPMPPAMQAPGSIY---NPGYAG-RPLFEDQ 80
A + L+L GCA IP P+ Q SA P P A GSI+ P G +PLFED+
Sbjct: 10 AISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINYGYQPLFEDR 69

Query: 81 RPRNVGDILTIVIAENINATKSSGANTNRQGNTSFDVPTAG-FLGGLF--NKANLSAQGA 137
RPRN+GD LTIV+ EN++A+KSS AN +R G T+F T +L GLF +A++ A G
Sbjct: 70 RPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGG 129

Query: 138 NKFAATGGASAANTFNGTITVTVTNVLPNGNLVVSGEKQMLINQGNEFVRFSGIVNPNTI 197
N F GGA+A+NTF+GT+TVTV VL NGNL V GEKQ+ INQG EF+RFSG+VNP TI
Sbjct: 130 NTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTI 189

Query: 198 SGQNSVYSTQVADARIEYSAKGYINEAETMGWLQRFFLNIAP 239
SG N+V STQVADARIEY GYINEA+ MGWLQRFFLN++P
Sbjct: 190 SGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSP 231


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0285FLGPRINGFLGI371e-129 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 371 bits (953), Expect = e-129
Identities = 164/392 (41%), Positives = 225/392 (57%), Gaps = 27/392 (6%)

Query: 11 RVVRPLVAARRRAAACCALAACMLALAFAPAAARAERLKDLAQIQGVRDNPLIGYGLVVG 70
RV+R + AA +A L+ PA A R+KD+A +Q RDN LIGYGLVVG
Sbjct: 2 RVLRIIAAALVFSALPF--------LSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVG 53

Query: 71 LDGTGDQTMQTPFTTQTLANMLANLGISINNGSANGGGSSAMTNMQLKNVAAVMVTATLP 130
L GTGD +PFT Q++ ML NLGI+ G +N KN+AAVMVTA LP
Sbjct: 54 LQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQSN-----------AKNIAAVMVTANLP 102

Query: 131 PFARPGEAIDVTVSSLGNAKSLRGGTLLLTPLKGADGQVYALAQGNMAVGGAGASANGSR 190
PFA PG +DVTVSSLG+A SLRGG L++T L GADGQ+YA+AQG + V G A + +
Sbjct: 103 PFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQGALIVNGFSAQGDAAT 162

Query: 191 VQVNQLAAGRIAGGAIVERSVPNAVAQMNGVLQLQLNDMDYGTAQRIVSAVNS----SFG 246
+ + R+ GAI+ER +P+ L LQL + D+ TA R+ VN+ +G
Sbjct: 163 LTQGVTTSARVPNGAIIERELPSKFKDSV-NLVLQLRNPDFSTAVRVADVVNAFARARYG 221

Query: 247 AGTATALDGRTIQLTAPADSAQQVAFMARLQNLEVSPERAAAKVILNARTGSIVMNQMVT 306
A D + I + P + MA ++NL V + AKV++N RTG+IV+ V
Sbjct: 222 DPIAEPRDSQEIAVQKPRVA-DLTRLMAEIENLTVETD-TPAKVVINERTGTIVIGADVR 279

Query: 307 LQNCAVAHGNLSVVVNTQPVVSQPGPFSNGQTVVAQQSQIQLKQDNGSLRMVTAGANLAD 366
+ AV++G L+V V P V QP PFS GQT V Q+ I Q+ + + G +L
Sbjct: 280 ISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGSKV-AIVEGPDLRT 338

Query: 367 VVKALNSLGATPADLMSILQAMKAAGALRADL 398
+V LNS+G +++ILQ +K+AGAL+A+L
Sbjct: 339 LVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0286FLGFLGJ2273e-75 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 227 bits (579), Expect = 3e-75
Identities = 124/297 (41%), Positives = 173/297 (58%), Gaps = 15/297 (5%)

Query: 15 ALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSSSSKMY 74
A D Q + L++KA P ++ VA Q + MF QMMLKSMRDA P DGL S +++Y
Sbjct: 12 AWDAQSLNELKAKA-GEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDGLFSSEHTRLY 70

Query: 75 TSMLDQQLAQQMSS-KGIGVADALTKQLLRNANVAPDAQGEGGLAAMNALAKAYANSNGA 133
TSM DQQ+AQQM++ KG+G+A+ + KQ+ + ++ + Y N +
Sbjct: 71 TSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLETVVRYQNQALS 130

Query: 134 PGNGALAGTRGYSAASALTPPLKGNGNSAQADAFVEKMALAAQAASATTGIPARFIVGQA 193
P + + AF+ +++L AQ AS +G+P I+ QA
Sbjct: 131 ------------QLVQKAVPRNYDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQA 178

Query: 194 ALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKPHRVVAQFRAYDS 253
ALESGWG+R+IR NGE SYN+FG+KA+ W G TTEY NG+ +V A+FR Y S
Sbjct: 179 ALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSS 238

Query: 254 YEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYAKKLISIMQQI 310
Y A++DY LL NPRYA+V A +AE A +Q AGYATDPHYA+KL +++QQ+
Sbjct: 239 YLEALSDYVGLLTRNPRYAAVTTAA-SAEQGAQALQDAGYATDPHYARKLTNMIQQM 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0288FLGHOOKAP12314e-70 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 231 bits (591), Expect = 4e-70
Identities = 162/444 (36%), Positives = 253/444 (56%), Gaps = 12/444 (2%)

Query: 3 NTLMNLGVSGLNAALWGLTTTGQNISNAATPGYSVERPVYAEASGQYTSSGYLPQGVSTV 62
++L+N +SGLNAA L T NIS+ GY+ + + A+A+ + G++ GV
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVS 60

Query: 63 TVERQYNQYLSNQLNAAQTQGSSLSTYYTLVAQLNNYVGSPTAGIATAITNYFTGLQTVA 122
V+R+Y+ +++NQL AAQTQ S L+ Y +++++N + + T+ +AT + ++FT LQT+
Sbjct: 61 GVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLV 120

Query: 123 NNAADPSARQTAMSNAQTLASQLVAAGQQYSQLRQSVNSQLTDTVTQINSYTSQIAQLNE 182
+NA DP+ARQ + ++ L +Q Q + VN + +V QIN+Y QIA LN+
Sbjct: 121 SNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLND 180

Query: 183 QIA--SASSQGQPPNQLLDQRDLAVSKLSQLAGVQV-VQSNGNYSVFLSGGQPLVVGNAS 239
QI+ + G PN LLDQRD VS+L+Q+ GV+V VQ G Y++ ++ G LV G+ +
Sbjct: 181 QISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTA 240

Query: 240 YQLATVASPSDPSELTI-VSKGVAGSAQPGPTQYLPDVSLTGGALGGLLAFRSQTLDPAQ 298
QLA V S +DPS T+ G AG+ + +P+ L G+LGG+L FRSQ LD +
Sbjct: 241 RQLAAVPSSADPSRTTVAYVDGTAGNIE------IPEKLLNTGSLGGILTFRSQDLDQTR 294

Query: 299 AQLGALAVSFASQVNAQNALGVDMSGNPGGSLFAVGAPAVYANQNNTGSATLSVSFVDGT 358
LG LA++FA N Q+ G D +G+ G FA+G PAV N N G + + D +
Sbjct: 295 NTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDAS 354

Query: 359 QPTTSDYALSYDGAKYTLTDRATGSVVGTATPSSTPPTMTIGGLKLSLSSTPNAGDSFTV 418
+DY +S+D ++ +T R + T TP + + GL+L+ + TP DSFT+
Sbjct: 355 AVLATDYKISFDNNQWQVT-RLASNTTFTVTPDAN-GKVAFDGLELTFTGTPAVNDSFTL 412

Query: 419 LPTRGALDGFSLATANGSAIAAAS 442
P A+ + + + IA AS
Sbjct: 413 KPVSDAIVNMDVLITDEAKIAMAS 436



Score = 83.1 bits (205), Expect = 9e-19
Identities = 46/105 (43%), Positives = 66/105 (62%)

Query: 561 GTNDGRNALALSQLVNSKTMNNGTTTLTGAYAGYVNAIGNAASQLKASSAAQTALVGQIT 620
G +D RN AL L ++ G + AYA V+ IGN + LK SSA Q +V Q++
Sbjct: 441 GDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLS 500

Query: 621 QAQQSVSGVNQNEEAANLMQYQQLYQANAKVIQTANSVFQTVLGL 665
QQS+SGVN +EE NL ++QQ Y ANA+V+QTAN++F ++ +
Sbjct: 501 NQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0289FLAGELLIN424e-06 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 42.0 bits (98), Expect = 4e-06
Identities = 51/390 (13%), Positives = 110/390 (28%), Gaps = 12/390 (3%)

Query: 16 MNDQQAQIAQLYQQVSSGISLTTPADNPLAAAQAVQLSATSATLAQYTQNQTIVQTALQT 75
+N Q+ ++ +++SSG+ + + D+ A A + ++ L Q ++N + QT
Sbjct: 17 LNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNANDGISIAQT 76

Query: 76 EDTTLTSVNDVLNAAYQALMHAGDGGLSDSDRAALAAQIQGSRDHLLTLANTADGAGNYL 135
+ L +N+ L + + A +G SDSD ++ +IQ + + ++N G +
Sbjct: 77 TEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKV 136

Query: 136 FAGFQPTTQPFSNKPGGGVTYAGDYGARAVQIADTRTVSQGDNGANVFMSVPFLGSLPVP 195
+ G +T + + S G +G NV
Sbjct: 137 LSQDNQMKIQVGANDGETIT---------IDLQKIDVKSLGLDGFNVNGPKEATVGDLKS 187

Query: 196 AAGASNTGTGTIGAVSITNPSDPTNTHQFTITFGGTAAAPTYTVTDNSVTPPPTTAAQAY 255
+ + + T + +T T A+
Sbjct: 188 SFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLT---TDDAENN 244

Query: 256 SSGQGINLGGQTVAVSGKPAVGDTFTVTPAPQAGTDVFATLDTVIAALKSPVGNSQTAST 315
++ T + A+ T G T
Sbjct: 245 TAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTIN 304

Query: 316 ALTNTMATASTKLMNTMTNVLTVQASVGGRLQEVKAMQAVTTTNTLQTTNSLSNLTDTNL 375
T+ A + T+Q+S V ++ + +
Sbjct: 305 GEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAV 364

Query: 376 PAAISQFLQLQNSLSAAQKAFVQMQNLSLF 405
+ + A V + ++F
Sbjct: 365 KGESKITVNGAEYTANAAGDKVTLAGKTMF 394


5BURPS1106A_0337BURPS1106A_0349Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0337-2113.395404UDP-N-acetylglucosamine pyrophosphorylase
BURPS1106A_0339-2113.563254hypothetical protein
BURPS1106A_0340-1113.019051C32 tRNA thiolase
BURPS1106A_03410144.257229dihydroneopterin aldolase
BURPS1106A_03420145.108784hypothetical protein
BURPS1106A_03430142.755703hypothetical protein
BURPS1106A_0344-1152.893991hypothetical protein
BURPS1106A_0345-1163.815482hypothetical protein
BURPS1106A_0346-2153.619280hypothetical protein
BURPS1106A_0347-1143.714144fructokinase
BURPS1106A_0348-2144.045671N-acylglucosamine 2-epimerase family protein
BURPS1106A_0349-1124.215581LacI family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0337SSBTLNINHBTR290.021 Streptomyces subtilisin inhibitor signature.
		>SSBTLNINHBTR#Streptomyces subtilisin inhibitor signature.

Length = 144

Score = 29.0 bits (64), Expect = 0.021
Identities = 21/44 (47%), Positives = 23/44 (52%), Gaps = 3/44 (6%)

Query: 21 VLHPLAGRPLLSHVIDTARALAPSRLVVVIGHGAEQVRAAVAAP 64
V PLAG L S A APS LV+ +GHG AA AAP
Sbjct: 18 VCGPLAGASLASPATAPASLYAPSALVLTVGHGES---AATAAP 58


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0348cloacin357e-04 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 34.7 bits (79), Expect = 7e-04
Identities = 16/57 (28%), Positives = 21/57 (36%)

Query: 424 GRGANAGGGAGDDDRAPRAAHDSGRGGGGKGGGKGGGKGGGTDDHGHRGEGDAADGA 480
G GA+ G G ++ SG GG G GG G + G +A A
Sbjct: 30 GGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86



Score = 30.5 bits (68), Expect = 0.018
Identities = 15/44 (34%), Positives = 16/44 (36%)

Query: 421 DDSGRGANAGGGAGDDDRAPRAAHDSGRGGGGKGGGKGGGKGGG 464
D SG + G SG G GG G GGG G G
Sbjct: 35 DGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTG 78



Score = 30.1 bits (67), Expect = 0.026
Identities = 16/65 (24%), Positives = 21/65 (32%)

Query: 417 ASTRDDSGRGANAGGGAGDDDRAPRAAHDSGRGGGGKGGGKGGGKGGGTDDHGHRGEGDA 476
S + G GG D + ++ GG G G GGG G G G +
Sbjct: 16 TSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGS 75

Query: 477 ADGAG 481
G
Sbjct: 76 GTGGN 80


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0349HTHTETR280.043 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 28.1 bits (62), Expect = 0.043
Identities = 17/136 (12%), Positives = 34/136 (25%), Gaps = 8/136 (5%)

Query: 2 GTTIRDVAQAANVSIGTVSRALKNQPGLSEATRARIVE-----IAHRMNYDPTQLRPRIK 56
T++ ++A+AA V+ G + K++ L P ++
Sbjct: 31 STSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLR 90

Query: 57 -RLTFLLHRQHNNFATTPFFSHVLHGVEDACRERGIVPSLLTTGPTDDVIRQMRPHAPDA 115
L +L + H E E +V + ++
Sbjct: 91 EILIHVLESTVTEERRRLLMEIIFHKCEFV-GEMAVVQQAQRNLCL-ESYDRIEQTLKHC 148

Query: 116 IAVAGFMEPETLEALA 131
I A
Sbjct: 149 IEAKMLPADLMTRRAA 164


6BURPS1106A_0374BURPS1106A_0388Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0374019-3.227287leucine-responsive regulatory protein
BURPS1106A_0375020-3.332870AzlC family protein
BURPS1106A_0376121-3.932484AzlD family protein
BURPS1106A_0377122-3.104095alpha/beta hydrolase
BURPS1106A_0378224-4.139425SCO1/SenC family protein
BURPS1106A_0379224-3.604726kelch domain-containing protein
BURPS1106A_0380234-2.974982hypothetical protein
BURPS1106A_0381231-4.431363hypothetical protein
BURPS1106A_0382331-4.760978dihydrodipicolinate synthase
BURPS1106A_0383437-7.142707sensor histidine kinase
BURPS1106A_0384434-5.983175hypothetical protein
BURPS1106A_0385332-5.048430hypothetical protein
BURPS1106A_0386-319-3.090750hypothetical protein
BURPS1106A_0387017-0.264376hypothetical protein
BURPS1106A_03882150.005266Fis family regulatory protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0388HTHFIS635e-15 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 62.9 bits (153), Expect = 5e-15
Identities = 22/59 (37%), Positives = 40/59 (67%), Gaps = 1/59 (1%)

Query: 55 THRDLKNAIAKGMFRMDLFHRIAVTAASIPDLRERIEDLPSLIAYWLARLCERHGLPPR 113
T++DLK +I +G+FR DL++R+ V +P LR+R ED+P L+ +++ + + GL +
Sbjct: 279 TNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAE-KEGLDVK 336


7BURPS1106A_0436BURPS1106A_0452Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0436193.131008hypothetical protein
BURPS1106A_04370103.017630IclR family transcriptional regulator
BURPS1106A_04380101.827317fumarylacetoacetate hydrolase family protein
BURPS1106A_04391101.732237enoyl-CoA hydratase
BURPS1106A_04401132.370034hypothetical protein
BURPS1106A_0441-1142.062418patatin family phospholipase
BURPS1106A_04420123.402913hypothetical protein
BURPS1106A_0443-1134.345018hypothetical protein
BURPS1106A_0444-2133.748907aut protein
BURPS1106A_0445-2133.726819hypothetical protein
BURPS1106A_0446-1143.092329pantothenate kinase
BURPS1106A_04470142.644115biotin--protein ligase
BURPS1106A_04481152.131052hypothetical protein
BURPS1106A_04491152.0695442,3-cyclic-nucleotide 2'phosphodiesterase
BURPS1106A_04502152.838487ABC transporter permease
BURPS1106A_04510142.804851ABC transporter ATP-binding protein
BURPS1106A_0452-2143.447732ABC transporter substrate-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0436PF03544347e-04 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 34.2 bits (78), Expect = 7e-04
Identities = 18/98 (18%), Positives = 28/98 (28%)

Query: 55 PVQVELLKPQPIERAPAPEKPAADRPRAAPKRAARASAPPAHAPRASAPVSSAAESSTES 114
P Q P+P+ +P + P+ AP + P P+ V
Sbjct: 62 PPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPV 121

Query: 115 SAESPAAASGTEPASAAGGQAAGATSGAAAGASGASAP 152
+ + T PA A ATS +
Sbjct: 122 ESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRA 159


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0445GPOSANCHOR300.002 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 30.4 bits (68), Expect = 0.002
Identities = 21/80 (26%), Positives = 29/80 (36%), Gaps = 8/80 (10%)

Query: 70 PALETAPLNAPGAAPAAASDSAPGSPAASAPASAVAPASMPASVAAPAAPA----PSSPP 125
A E A L A A+ + D+ PG+ A A + P AP PS+
Sbjct: 451 QAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKAPMKETKRQLPSTGE 510

Query: 126 AAQP----ARAPILPGASAA 141
A P A ++ A A
Sbjct: 511 TANPFFTAAALTVMATAGVA 530


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0446PF033092026e-67 Bvg accessory factor
		>PF03309#Bvg accessory factor

Length = 271

Score = 202 bits (516), Expect = 6e-67
Identities = 58/279 (20%), Positives = 102/279 (36%), Gaps = 47/279 (16%)

Query: 1 MCLLIDAGNSRIKWALADTARHFVTSGAFEHASDAPDWSTLPAPR------GAWISNVAG 54
M L ID N+ L G+ +HA W P I + G
Sbjct: 1 MLLAIDVRNTHTVVGLIS--------GSGDHAKVVQQWRIRTEPEVTADELALTIDGLIG 52

Query: 55 DAAAA---------------RIDALIEARWPALPRTVVRASAAQCGVTNGYAEPARLGSD 99
D A + ++E WP +P ++ G+ P +G+D
Sbjct: 53 DDAERLTGASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVR-TGIPLLVDNPKEVGAD 111

Query: 100 RWAGLIGAHAAFADEHLLIATFGTATTLEALRADGHFAGGLIAPGWALMMRSLGMHTAQL 159
R + A+ + +++ FG++ ++ + A G F GG IAPG + + +A L
Sbjct: 112 RIVNCLAAYHKYGTAAIVV-DFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAAL 170

Query: 160 PTVSIDAATNLLDELAENDAHAPFAIDTPHALSAGCLQAQAGLIE----RAWRDLEKAWQ 215
V + +++ + +T + AG + AGL++ R D++
Sbjct: 171 RRVELTRPRSVIGK------------NTVECMQAGAVFGFAGLVDGLVNRIRDDVDGFSG 218

Query: 216 APVRLVLSGGAADAIVRALTVPHTRHDTLVLTGLALIAH 254
A V +V +G A ++ L L L GL L+
Sbjct: 219 ADVAVVATGHTAPLVLPDLRTVEHYDRHLTLDGLRLVFE 257


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0447SECA290.027 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.1 bits (65), Expect = 0.027
Identities = 18/49 (36%), Positives = 22/49 (44%), Gaps = 4/49 (8%)

Query: 198 AAAEVDALRARDATLAGGLP----PVALAAVRAGATLTDTFAAALNALA 242
A+ V +R D L GG+ +A G TLT T A LNAL
Sbjct: 74 ASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALT 122


8BURPS1106A_0524BURPS1106A_0561Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_05240103.018002ABC transporter ATP-binding protein
BURPS1106A_05251103.281523hypothetical protein
BURPS1106A_05260102.389905hypothetical protein
BURPS1106A_0527-191.590338error-prone DNA polymerase
BURPS1106A_0528182.112208hypothetical protein
BURPS1106A_0529092.615269major facilitator transporter
BURPS1106A_05310103.334462hypothetical protein
BURPS1106A_0530-1123.161488fatty acid desaturase family protein
BURPS1106A_0532-1133.9275542,4-diaminobutyrate 4-transaminase
BURPS1106A_0533-1154.506613hypothetical protein
BURPS1106A_0534-2153.431477ABC-2 type transporter, permease
BURPS1106A_0535-2164.156039ABC transporter ATP-binding protein
BURPS1106A_0536-1154.354446syringomycin biosynthesis enzyme
BURPS1106A_0537-2144.304811UbiE/COQ5 family methlytransferase
BURPS1106A_0538-1144.065145citrate synthase-like protein
BURPS1106A_05390132.937102acyl-CoA dehydrogenase
BURPS1106A_05400143.528770hypothetical protein
BURPS1106A_05410143.381999AMP-binding protein
BURPS1106A_05420142.320783pyridoxal-dependent decarboxylase family
BURPS1106A_05432162.851072hypothetical protein
BURPS1106A_05442152.825528hypothetical protein
BURPS1106A_05451133.328232hypothetical protein
BURPS1106A_05462123.463410hypothetical protein
BURPS1106A_05472122.665249hypothetical protein
BURPS1106A_05483123.348045hypothetical protein
BURPS1106A_05494123.730409AMP-binding protein
BURPS1106A_05503143.501086LysR family transcriptional regulator
BURPS1106A_05512143.706962GntR family transcriptional regulator
BURPS1106A_05521133.561076N-acetylglucosamine-6-phosphate deacetylase
BURPS1106A_05530123.836289SIS domain-containing protein
BURPS1106A_0554-1123.362568PTS system glucose-glucoside (Glc) family
BURPS1106A_0555-2121.721873PTS system N-acetylglucosamine-specific
BURPS1106A_0556-2120.282900glycosyl hydrolase family protein
BURPS1106A_0557-212-1.920981hypothetical protein
BURPS1106A_0558-111-2.378478hypothetical protein
BURPS1106A_0559011-3.756568cyd operon protein YbgT
BURPS1106A_056009-3.886051cytochrome d ubiquinol oxidase, subunit II
BURPS1106A_056109-4.064921cytochrome d ubiquinol oxidase, subunit I
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0534ABC2TRNSPORT320.003 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 31.8 bits (72), Expect = 0.003
Identities = 33/155 (21%), Positives = 60/155 (38%), Gaps = 7/155 (4%)

Query: 163 YGEFFATGILIMAFMSIGVVSTA-TTIATLRERNTFKMYVCFPVSRF-VFLASLIVSRVI 220
Y F A G++ + M+ T + + T++ + + + L + +
Sbjct: 65 YTAFLAAGMVATSAMTAATFETIYAAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATK 124

Query: 221 LMLAASVTLMLAARYLFQVPLPLWSLRALRAIPVVLLGAAMLLSLGTLLASRARSLAAAE 280
LA + ++AA + SL L A+PV+ L SLG ++ + A S
Sbjct: 125 AALAGAGIGVVAAALGY---TQWLSL--LYALPVIALTGLAFASLGMVVTALAPSYDYFI 179

Query: 281 AWCNLIYFPLLFFSDLTIPLRAAPHWLRVVLLVLP 315
+ L+ P+LF S P+ P + LP
Sbjct: 180 FYQTLVITPILFLSGAVFPVDQLPIVFQTAARFLP 214


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0554PHPHTRNFRASE513e-175 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 513 bits (1322), Expect = e-175
Identities = 194/567 (34%), Positives = 312/567 (55%), Gaps = 7/567 (1%)

Query: 306 PNTLAGVCAAPGIAVGTLVRWDDAQIVPPELASGTPAAESRLLDRALAEVDAQLETTVRE 365
+ + G+ A+ G+A+ + + + + + E L AL + +L +
Sbjct: 2 HHKITGIAASSGVAIAKAFIHLEPNVDIEKTSITDVSTEIEKLTAALEKSKEELRAIKDQ 61

Query: 366 ASRRGAIGEAGIFAVHRVLLEDPALVDAARDLI-SLGKSAGYAWRETIRAQTAVLADVDD 424
+A IFA H ++L+DP LVD + I + +A YA +E ++ +D+
Sbjct: 62 TEASMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSMFESMDN 121

Query: 425 TLLAERAADLRDIDKRVLRAL-GYASASARELPAEAVLAAEEFTPSDLASLDRERVAALV 483
+ ERAAD+RD+ KRVL L G + S + E V+ AE+ TPSD A L+++ V
Sbjct: 122 EYMKERAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFA 181

Query: 484 MARGGATSHAAIIARQLGIPALVAVGDALYAIAQRTQVVVDASAGRLEYAPSALDVERAR 543
GG TSH+AI++R L IPA+V + I V+VD G + P+ +V+
Sbjct: 182 TDIGGRTSHSAIMSRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIVNPTEEEVKAYE 241

Query: 544 HERQRLAGVREANRRMSGEAALTRDGHRIEVAANIATLDDARVALDNGADAVGLLRTELM 603
+R ++ ++ GE + T+DG +E+AANI T D L NG + +GL RTE +
Sbjct: 242 EKRAAFEKQKQEWAKLVGEPSTTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFL 301

Query: 604 FIHRQAAPTASEHQQSYQSIVDALQGRTAIIRTLDVGADKEVDYLTLPPEPNPALGLRGI 663
++ R PT E ++Y+ +V + G+ +IRTLD+G DKE+ YL LP E NP LG R I
Sbjct: 302 YMDRDQLPTEEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYLQLPKELNPFLGFRAI 361

Query: 664 RLAQVRPDLLDDQLRGLLAVKPYGSVRILLPMVTDVGELVRIRKRIDD-----FARAMGR 718
RL + D+ QLR LL YG+++++ PM+ + EL + + + + + +
Sbjct: 362 RLCLEKQDIFRTQLRALLRASTYGNLKVMFPMIATLEELRQAKAIMQEEKDKLLSEGVDV 421

Query: 719 AQAVEVGVMIEVPSAALLADQLAQHADFLSIGTNDLTQYTLAMDRCQADLAAQADGLHPA 778
+ ++EVG+M+E+PS A+ A+ A+ DF SIGTNDL QYT+A DR ++ HPA
Sbjct: 422 SDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYHPA 481

Query: 779 VLRLVDATVRGAEKHGKWVGVCGALGGDPVAVPVLVGLGVTELSVDPVSVPGIKAQVRRL 838
+LRLVD ++ A GKWVG+CG + GD VA+P+L+GLG+ E S+ S+ ++Q+ +L
Sbjct: 482 ILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARSQLLKL 541

Query: 839 DYQLCRQRAQDLLALESAQAVRAASRE 865
+ + AQ L L++A+ V ++
Sbjct: 542 SKEELKPFAQKALMLDTAEEVEQLVKK 568


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0556cloacin310.022 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 30.8 bits (69), Expect = 0.022
Identities = 31/120 (25%), Positives = 48/120 (40%), Gaps = 8/120 (6%)

Query: 176 VVVDGAAPAVLRYDDTDDELRYVETLPADAQNNSPGNAPP--AAAQPVANRALPSVKRQR 233
V + G P+ + DD + + V +LPAD SP ++ P A V R + VK +R
Sbjct: 134 VALYGVLPSQIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDER 193

Query: 234 ALPGALDLRGVELTLPELPSAQVAALRERAGTLGLDGARVPVWGVVAPRRLPADIAVPGG 293
+ GV +++P + A ER G PV + PA + G
Sbjct: 194 QNISVVS--GVPMSVPVVD----AKPTERPGVFTASIPGAPVLNISVNNSTPAVQTLSPG 247


9BURPS1106A_0592BURPS1106A_0634Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0592211-2.875451ATP-dependent protease La
BURPS1106A_0593211-2.839356hypothetical protein
BURPS1106A_0594013-2.644196HPr kinase/phosphorylase
BURPS1106A_0595-114-2.703221PTS transporter subunit IIA-like
BURPS1106A_0596-213-1.698010ribosomal subunit interface protein
BURPS1106A_0597-113-0.472671RNA polymerase factor sigma-54
BURPS1106A_05980120.853465ABC transporter ATP-binding protein
BURPS1106A_05990100.918993hypothetical protein
BURPS1106A_06001111.055170hypothetical protein
BURPS1106A_06013101.5454063-deoxy-D-manno-octulosonate 8-phosphate
BURPS1106A_06023100.909908KpsF/GutQ family sugar isomerase
BURPS1106A_06034110.172247monovalent cation:proton antiporter-2 (CPA2)
BURPS1106A_0604016-0.964519adenine phosphoribosyltransferase
BURPS1106A_0605-114-1.068421LysE family translocator protein
BURPS1106A_0606-212-1.171871nudix hydrolase
BURPS1106A_0607-110-1.507287formyltetrahydrofolate deformylase
BURPS1106A_0608-110-1.721271hypothetical protein
BURPS1106A_0609-27-1.154305excinuclease ABC subunit A
BURPS1106A_0610315-0.989019major facilitator family transporter
BURPS1106A_0611520-0.578564single-stranded DNA-binding protein
BURPS1106A_0612524-1.539405dienelactone hydrolase family protein
BURPS1106A_0613529-3.196892hypothetical protein
BURPS1106A_0614528-3.754522Zn-dependent hydrolases, including glyoxylases
BURPS1106A_0615440-8.524997hypothetical protein
BURPS1106A_0616647-8.166844hypothetical protein
BURPS1106A_06171102.072807hypothetical protein
BURPS1106A_06180102.187533hypothetical protein
BURPS1106A_06190102.428251hypothetical protein
BURPS1106A_06200102.6209044-carboxymuconolactone decarboxylase
BURPS1106A_06211112.963725carboxymuconolactone decarboxylase family
BURPS1106A_06221112.939863FG-GAP/YD repeat-containing protein
BURPS1106A_0623-190.683009hypothetical protein
BURPS1106A_0624524-2.812929hypothetical protein
BURPS1106A_0625525-3.769261hypothetical protein
BURPS1106A_0626626-4.379434hypothetical protein
BURPS1106A_0627727-5.444898hypothetical protein
BURPS1106A_0628118-1.868144hypothetical protein
BURPS1106A_06292113.457420hypothetical protein
BURPS1106A_06301113.664750hypothetical protein
BURPS1106A_06310103.505716hypothetical protein
BURPS1106A_0632284.066503hypothetical protein
BURPS1106A_06332104.009090FHA domain-containing protein
BURPS1106A_06342103.566970serine/threonine protein kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0610TCRTETA853e-20 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 84.9 bits (210), Expect = 3e-20
Identities = 77/368 (20%), Positives = 143/368 (38%), Gaps = 31/368 (8%)

Query: 7 RATTSLAAIFALRMLGLFMIMPVFSVYAKTIPGGENVVL-VGIALGAYGVTQSLLYIFYG 65
R + + AL +G+ +IMPV + + +V GI L Y + Q G
Sbjct: 5 RPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLG 64

Query: 66 WASDKFGRKPVIAAGLLIFALGSFVAAFAHDITWIIVGRVIQGM-GAVSSAVLAFIADLT 124
SD+FGR+PV+ L A+ + A A + + +GR++ G+ GA + A+IAD+T
Sbjct: 65 ALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADIT 124

Query: 125 SEHNRTKAMAMVGGSIGMSFAVAIVGAPI--VFHWVGMSGLFAIVGALSVAAIGVVLWVV 182
R + + G + G + + F AL+ +++
Sbjct: 125 DGDERARHFGFMSACFGFGM---VAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL 181

Query: 183 PDAPRPVHVPAPFAEVLHNVELLRLNFGVLVLHATQTALFLVVPRLLVDGGLPVA----- 237
P++ + P E L+ + R G+ V+ A F+ + + G +P A
Sbjct: 182 PESHKGERRPLR-REALNPLASFRWARGMTVVAALMAVFFI----MQLVGQVPAALWVIF 236

Query: 238 ----SHWQ-----VYLPVMGL--AFVMMVPAIIVAEKQGRMKPVLLGGIAAILIGQLLLG 286
HW + L G+ + + VA + G + ++L G+ A G +LL
Sbjct: 237 GEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALML-GMIADGTGYILLA 295

Query: 287 VATHTILIVAAILFVYFLGFNILEASQPSLVSKLAPGSRKGAATGVYNTTQSIGLALGGV 346
AT + + V I + +++S+ R+G G S+ +G +
Sbjct: 296 FATRGWMAF--PIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPL 353

Query: 347 VGGVLLKH 354
+ +
Sbjct: 354 LFTAIYAA 361


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0611cloacin463e-08 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 45.9 bits (108), Expect = 3e-08
Identities = 29/71 (40%), Positives = 32/71 (45%), Gaps = 4/71 (5%)

Query: 109 GGRGGSGGGGGGGDDGGYGG----GGGGYGGGRDMERGGGGGRASGGGGAGARSGGGGGA 164
GG G G GGG D G+ GGG G G G G G G G +G SG GG
Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81

Query: 165 SRPSAPAGGGF 175
S +AP GF
Sbjct: 82 SAVAAPVAFGF 92



Score = 33.1 bits (75), Expect = 5e-04
Identities = 22/69 (31%), Positives = 24/69 (34%), Gaps = 3/69 (4%)

Query: 109 GGRGGSGGGGGGGDDGGYGGGGGGYGGGRDMER---GGGGGRASGGGGAGARSGGGGGAS 165
G + G GG G GGG G G E GGG G GG GGG +
Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70

Query: 166 RPSAPAGGG 174
GG
Sbjct: 71 SGGGSGTGG 79



Score = 32.4 bits (73), Expect = 0.001
Identities = 21/62 (33%), Positives = 24/62 (38%), Gaps = 6/62 (9%)

Query: 113 GSGGGGGGGDDGGYGGGGGGYGGGRDMERGGGGGRASGGGGAGARSGGGGGASRPSAPAG 172
G G G G GG G G GG G GGG +GG + + G S P
Sbjct: 47 GGGSGSGIHWGGGSGHGNGGGNGN------SGGGSGTGGNLSAVAAPVAFGFPALSTPGA 100

Query: 173 GG 174
GG
Sbjct: 101 GG 102



Score = 29.7 bits (66), Expect = 0.007
Identities = 17/53 (32%), Positives = 18/53 (33%)

Query: 109 GGRGGSGGGGGGGDDGGYGGGGGGYGGGRDMERGGGGGRASGGGGAGARSGGG 161
GG G GGG G GG G GG + G G GG G
Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0622SALSPVBPROT606e-11 Salmonella virulence plasmid 65kDa B protein signature.
		>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature.

Length = 591

Score = 60.1 bits (145), Expect = 6e-11
Identities = 55/208 (26%), Positives = 81/208 (38%), Gaps = 41/208 (19%)

Query: 12 LNLPSGGGSVSGDGGDFSVDLNTGTATLKFDLTVPAGPNGITPPHTLQYSAGAGDGAFGI 71
LP GG ++S G D G A++ L + A G P L YS+G G+G FG+
Sbjct: 18 PFLPKGGKALSQSGPD-------GLASITLPLPISAE-RGFAPALALHYSSGGGNGPFGV 69

Query: 72 GWSLGLMTIRRR-----------------------ITPATGAAEPAPPGACSLVGVGELV 108
GWS M+I R T +TG A P P + V
Sbjct: 70 GWSCATMSIARSTSHGVPQYNDSDEFLGPDGEVLVQTLSTGDA-PNPVTCFAYGDVSFPQ 128

Query: 109 DMGARRFRPIVDATGLLIEFTGAS------WTATDKTDTQYTLGTSANARIG---GGALP 159
R++P +++ +E+ + W D + LG +A AR+ +
Sbjct: 129 SYTVTRYQPRTESSFYRLEYWVGNSNGDDFWLLHDSNGILHLLGKTAAARLSDPQAASHT 188

Query: 160 AAWLVDRCADSAGNAIAYTWLDVGGARV 187
A WLV+ AG I Y++L G V
Sbjct: 189 AQWLVEESVTPAGEHIYYSYLAENGDNV 216


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0623PF05616350.001 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 35.1 bits (80), Expect = 0.001
Identities = 28/80 (35%), Positives = 32/80 (40%), Gaps = 9/80 (11%)

Query: 164 SQGNSAASSVAVTRAKAVDAAVVGAFSPPQMPNPSALPAANP--NAAPSTTPGFHPAPGV 221
SQGN+ + R D A +P P P PA NP N AP+ PG P P
Sbjct: 299 SQGNTTVDVQVIPRP---DLTPGSAEAPNAQPLPEVSPAENPANNPAPNENPGTRPNPEP 355

Query: 222 MPPRGIDLAPAALTSLKIQP 241
P DL P A QP
Sbjct: 356 DP----DLNPDANPDTDGQP 371


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0634YERSSTKINASE340.004 Yersinia serine/threonine protein kinase signature.
		>YERSSTKINASE#Yersinia serine/threonine protein kinase signature.

Length = 732

Score = 33.9 bits (77), Expect = 0.004
Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 2/42 (4%)

Query: 149 QVLDGLAHAHANGVVHRDLKPQNVMVTTRDGEPCAKILDFGI 190
++LD H GVVH D+KP NV+ GEP ++D G+
Sbjct: 253 RLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPV--VIDLGL 292


10BURPS1106A_0761BURPS1106A_0815Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_07612131.731255hypothetical protein
BURPS1106A_07623122.535770FAD binding domain-containing protein
BURPS1106A_07630163.010821Na+/H+-dicarboxylate symporter
BURPS1106A_0764-2113.336706LysR substrate binding domain-containing
BURPS1106A_0765-192.919931tRNA 2-selenouridine synthase
BURPS1106A_0766-1103.219761hypothetical protein
BURPS1106A_07670103.366567hypothetical protein
BURPS1106A_0769-1123.036572hypothetical protein
BURPS1106A_07680103.359896ABC transporter ATP-binding protein
BURPS1106A_0770-1113.104887ABC transporter permease
BURPS1106A_07710163.279470hypothetical protein
BURPS1106A_07721133.749140hypothetical protein
BURPS1106A_0773082.754711protein-L-isoaspartate O-methyltransferase
BURPS1106A_0774-192.997885hypothetical protein
BURPS1106A_0775-193.890408nicotinate phosphoribosyltransferase
BURPS1106A_0776-1104.404894hypothetical protein
BURPS1106A_0777-2103.323915phosphoribosyl transferase domain-containing
BURPS1106A_0778-2103.503578transglycosylase
BURPS1106A_0779-2103.742798hypothetical protein
BURPS1106A_0780-1113.803166cytochrome c family protein
BURPS1106A_0781-2113.476724hypothetical protein
BURPS1106A_0782-2113.342360cytochrome c oxidase subunit III:cytochrome c
BURPS1106A_07830114.048214cytochrome c oxidase subunit II
BURPS1106A_0784-1123.712615thiamine pyrophosphate protein
BURPS1106A_07850104.452811mandelate racemase/muconate lactonizing enzyme
BURPS1106A_0786174.413060hypothetical protein
BURPS1106A_0787074.241158hypothetical protein
BURPS1106A_0788083.337175GMC oxidoreductase
BURPS1106A_0789092.232197hypothetical protein
BURPS1106A_0790-1121.174534hypothetical protein
BURPS1106A_0791-2120.101221hypothetical protein
BURPS1106A_0792-212-0.295911penicillin amidase
BURPS1106A_0793-214-2.906156LysR family transcriptional regulator
BURPS1106A_0794-116-3.711097sensory box histidine kinase/response regulator
BURPS1106A_0795124-4.938456hypothetical protein
BURPS1106A_0796532-6.988039response regulator
BURPS1106A_07971046-10.376771hypothetical protein
BURPS1106A_0798846-11.562869hypothetical protein
BURPS1106A_08001051-12.858967hypothetical protein
BURPS1106A_0799847-13.194414phage integrase family protein
BURPS1106A_08011045-12.902457hypothetical protein
BURPS1106A_08021039-9.352536hypothetical protein
BURPS1106A_08031033-8.025256hypothetical protein
BURPS1106A_08041134-7.044663hypothetical protein
BURPS1106A_0805721-5.865181hypothetical protein
BURPS1106A_0806516-3.140330hypothetical protein
BURPS1106A_0807414-1.710186hypothetical protein
BURPS1106A_0808213-1.845160phage integrase family site specific
BURPS1106A_0810010-0.250802*hypothetical protein
BURPS1106A_0811-19-0.363354major facilitator family transporter
BURPS1106A_0812110-0.424702sensor histidine kinase
BURPS1106A_0813213-1.866917DNA-binding response regulator
BURPS1106A_0814214-2.043304recombinase A
BURPS1106A_0815215-0.994080recombination regulator RecX
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0781V8PROTEASE336e-04 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 33.1 bits (75), Expect = 6e-04
Identities = 11/30 (36%), Positives = 14/30 (46%)

Query: 147 NREPNAPGEPDELGELDELGEPDVPDEPDD 176
P+ P PD DE PD P+ PD+
Sbjct: 292 PDNPDNPNNPDNPNNPDEPNNPDNPNNPDN 321


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0794HTHFIS579e-11 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 57.1 bits (138), Expect = 9e-11
Identities = 34/122 (27%), Positives = 52/122 (42%), Gaps = 15/122 (12%)

Query: 484 RALVVDDNENARETLGAMLATLGIRVDLRGTGKEGLRCFGECQHDIVVLDLELPDISGFE 543
LV DD+ R L L+ G V + R D+VV D+ +PD + F+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 544 VAEQIRWATSSDAARKTTILGVSAYES------ALLKGDHAIFDAFIPKPIHLDTLGGIV 597
+ +I+ A +L +SA + A KG +D ++PKP L L GI+
Sbjct: 65 LLPRIK-----KARPDLPVLVMSAQNTFMTAIKASEKG---AYD-YLPKPFDLTELIGII 115

Query: 598 SR 599
R
Sbjct: 116 GR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0796HTHFIS379e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 37.1 bits (86), Expect = 9e-05
Identities = 22/125 (17%), Positives = 47/125 (37%), Gaps = 12/125 (9%)

Query: 266 ARIAVVDDSPDVAETICEYFAEKGVAAIAYYDSVSFRKALEVEDFDGYILDWLLGEETAA 325
A I V DD + + + + G ++ + + + D D + D ++ +E A
Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63

Query: 326 PLVRGIRASENADAPIFLLTGKISTGEASEDEIADIVSSFNARCEE---KPVRLPILFAE 382
L+ I+ D P+ +++ ++ + + + KP L L
Sbjct: 64 DLLPRIK-KARPDLPVLVMSA--------QNTFMTAIKASEKGAYDYLPKPFDLTELIGI 114

Query: 383 VARAL 387
+ RAL
Sbjct: 115 IGRAL 119


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0811TCRTETA358e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 34.8 bits (80), Expect = 8e-04
Identities = 47/264 (17%), Positives = 93/264 (35%), Gaps = 28/264 (10%)

Query: 77 AIVFGRLGDLVGRKHTFLITIVIMGISTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALG 136
A V G L D GR+ L+++ + ++ P V++I R++ G+ G
Sbjct: 60 APVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAP-------FLWVLYIG-RIVAGIT-G 110

Query: 137 GEYGGAATYVAEHAPSHRRGFYTSWIQTTATLGLFLSLLVILGVRTAIGEEAFGSWGWRV 196
A Y+A+ R + ++ G+ + G +G G +
Sbjct: 111 ATGAVAGAYIADITDGDERARHFGFMSACFGFGM------VAG--PVLGGLM-GGFSPHA 161

Query: 197 PFVASILLLAVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFGQWKNLKIVILALIGLT 256
PF A+ L ++ L + + + PL + + L
Sbjct: 162 PFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASF-----RWARGMTVVAALM 216

Query: 257 AGQAVVWYTGQFYA---LFFLTQTLKVDGASANILIALALLIGTPF-FVFFGSLSDRIGR 312
A ++ GQ A + F D + I +A ++ + + G ++ R+G
Sbjct: 217 AVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE 276

Query: 313 KPIILAGCLIAALTYFPLFKALTH 336
+ ++ G +IA T + L T
Sbjct: 277 RRALMLG-MIADGTGYILLAFATR 299



Score = 34.8 bits (80), Expect = 8e-04
Identities = 17/42 (40%), Positives = 24/42 (57%)

Query: 287 ILIALALLIGTPFFVFFGSLSDRIGRKPIILAGCLIAALTYF 328
IL+AL L+ G+LSDR GR+P++L AA+ Y
Sbjct: 47 ILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYA 88


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0812PF06580485e-08 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 47.9 bits (114), Expect = 5e-08
Identities = 49/229 (21%), Positives = 86/229 (37%), Gaps = 53/229 (23%)

Query: 300 LAGLRTQAEF-ALRHEVNA-------DVARSLEQIATSSEQAARLVTQLLALARAENRAT 351
+A + +A+ AL+ ++N + R+L I +A ++T L L R
Sbjct: 154 MASMAQEAQLMALKAQINPHFMFNALNNIRAL--ILEDPTKAREMLTSLSELMRY----- 206

Query: 352 GLTFEPVEIASLARQ--AVRDWV---QAALAKQMDLGYEGPDTDAPLRIDGQPVMLREML 406
L + SLA + V ++ ++ + +++ P ML +
Sbjct: 207 SLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQINPAIMDVQV---PPML---V 260

Query: 407 GNLIDNAIRY----TPAGGRITVRVRAERAAGAVHLEVEDTGPGIPPNERERVVERFYRI 462
L++N I++ P GG+I ++ + G V LEVE+TG N +E
Sbjct: 261 QTLVENGIKHGIAQLPQGGKILLKGTKDN--GTVTLEVENTGSLALKNTKE--------- 309

Query: 463 LGREGDGSGLGLAIVRE-IVAQHGGTLTIDDNVYQTSPRLAGTLVRVSI 510
+G GL VRE + +G I S + V I
Sbjct: 310 ------STGTGLQNVRERLQMLYGTEAQIK-----LSEKQGKVNAMVLI 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0813HTHFIS996e-26 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 98.8 bits (246), Expect = 6e-26
Identities = 35/118 (29%), Positives = 64/118 (54%), Gaps = 1/118 (0%)

Query: 2 RILIAEDDSILADGLTRSLRQSGYAVDHVRNGVEADTALSMQTFDLLILDLGLPRMSGLE 61
IL+A+DD+ + L ++L ++GY V N ++ DL++ D+ +P + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 VLRRLRARNSNLPVLILTAADSVDERVKGLDLGADDYMAKPFALNE-LEARVRALTRR 118
+L R++ +LPVL+++A ++ +K + GA DY+ KPF L E + RAL
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122


11BURPS1106A_0880BURPS1106A_0893Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_08800123.300070carbohydrate ABC transporter ATP-binding
BURPS1106A_08810123.961602hypothetical protein
BURPS1106A_0882-1105.532048hypothetical protein
BURPS1106A_08830135.373429LysR family transcriptional regulator
BURPS1106A_08840135.328961esterase
BURPS1106A_08850135.090160major facilitator family transporter
BURPS1106A_08860124.611254transcriptional regulator
BURPS1106A_08871124.942934xylulokinase
BURPS1106A_08882124.538615mannitol dehydrogenase
BURPS1106A_08890124.871346LysR family transcriptional regulator
BURPS1106A_08901124.516097benzoylformate decarboxylase
BURPS1106A_08912123.945604vanillin dehydrogenase
BURPS1106A_08922123.4904932-dehydropantoate 2-reductase
BURPS1106A_08932112.434614hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0880PF05272300.021 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.021
Identities = 14/35 (40%), Positives = 17/35 (48%)

Query: 50 VVFVGPSGCGKSTLMRMIAGLEEISGGELLIDGAK 84
VV G G GKSTL+ + GL+ S I K
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGK 633


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0881PF06776300.019 Invasion associated locus B
		>PF06776#Invasion associated locus B

Length = 214

Score = 29.5 bits (66), Expect = 0.019
Identities = 11/49 (22%), Positives = 15/49 (30%), Gaps = 2/49 (4%)

Query: 1 MKTGRRHFVRSVASASAALAAAAWSPARAAIDAPASPATALSLTPGRWS 49
+ + RR R+ A A A A A A+ G W
Sbjct: 38 LASCRRLARRNGARLMLAGAMAI--ALSFGWSDRADAQGAVRSVHGDWQ 84



Score = 28.7 bits (64), Expect = 0.038
Identities = 7/37 (18%), Positives = 13/37 (35%)

Query: 10 RSVASASAALAAAAWSPARAAIDAPASPATALSLTPG 46
+++ A L+ S R A A A ++
Sbjct: 25 KAIQMGPAELSPMLASCRRLARRNGARLMLAGAMAIA 61


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0884BLACTAMASEA300.018 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 29.8 bits (67), Expect = 0.018
Identities = 11/35 (31%), Positives = 15/35 (42%)

Query: 57 REDALFRFASVSKPIVSAAAMRAVAAGKLDLDASI 91
R D F S K ++ A + V AG L+ I
Sbjct: 57 RADERFPMMSTFKVVLCGAVLARVDAGDEQLERKI 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0885TCRTETB363e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 35.6 bits (82), Expect = 3e-04
Identities = 31/155 (20%), Positives = 59/155 (38%), Gaps = 5/155 (3%)

Query: 26 LLALATAGFITIVTEALPAGLLPLMGRDLRVSDALVGQLVTVYAAGSIVAAMPLVAATRG 85
L+ L F +++ E + LP + D A + T + + +
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 86 MRRRPLLLAALAGFVVANTATAASPYYAPVLV-ARCVAGVSAGLLWALLAGYASRMVDAR 144
+ + LLL + + + +L+ AR + G A AL+ +R +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 145 QRGRAIAIAMLGAPVAMSVGI-PL-GTALGAALGW 177
RG+A ++G+ VAM G+ P G + + W
Sbjct: 136 NRGKAFG--LIGSIVAMGEGVGPAIGGMIAHYIHW 168


12BURPS1106A_0935BURPS1106A_0960Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0935-293.102357hypothetical protein
BURPS1106A_09360124.262007hypothetical protein
BURPS1106A_0937-2114.155977chromate transporter
BURPS1106A_0938-1102.703828cyclic nucleotide-binding protein
BURPS1106A_0939-183.080720hypothetical protein
BURPS1106A_09403101.8870192-dehydropantoate 2-reductase
BURPS1106A_09412111.227705hypothetical protein
BURPS1106A_09421103.019814hypothetical protein
BURPS1106A_09432123.030211cyclic diguanylate phosphodiesterase
BURPS1106A_09441133.803886hypothetical protein
BURPS1106A_09451112.546941DHA2 family drug:H+ antiporter-1
BURPS1106A_09473123.521087hypothetical protein
BURPS1106A_09463133.330936citrate synthase family protein
BURPS1106A_09484211.486388hypothetical protein
BURPS1106A_09506342.127089hypothetical protein
BURPS1106A_09496352.218263GntR family transcriptional regulator
BURPS1106A_09523241.202216aldo/keto reductase
BURPS1106A_09515251.070548hypothetical protein
BURPS1106A_09534231.292552hypothetical protein
BURPS1106A_0954220-0.165391hypothetical protein
BURPS1106A_0955011-1.842842hypothetical protein
BURPS1106A_0956-19-1.127003elongation factor G
BURPS1106A_0957-19-1.170809hypothetical protein
BURPS1106A_095808-1.968083RNA pseudouridine synthase family protein
BURPS1106A_0960-17-3.197120isocitrate dehydrogenase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0945TCRTETB1383e-38 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 138 bits (349), Expect = 3e-38
Identities = 92/408 (22%), Positives = 171/408 (41%), Gaps = 15/408 (3%)

Query: 17 VMLWLVATGFFMQTLDATIVNTALPSMAASLGESPLRMQSVVIAYSLTMAVMIPVSGWLA 76
+++WL FF L+ ++N +LP +A + P V A+ LT ++ V G L+
Sbjct: 15 ILIWLCILSFF-SVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLS 73

Query: 77 DTLGTRRVFFSAILIFTLGSLLCANAHT-LPLLVAFRVIQGVGGAMLLPVGRLAVLRTFP 135
D LG +R+ I+I GS++ H+ LL+ R IQG G A + + V R P
Sbjct: 74 DQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIP 133

Query: 136 AERYLPALSFVAIPGLIGPLIGPTLGGWLVKIASWHWIFLINVPVGIAGCIATFYSMPDS 195
E A + +G +GP +GG + HW +L+ +P+ + +
Sbjct: 134 KENRGKAFGLIGSIVAMGEGVGPAIGGMIAH--YIHWSYLLLIPMITIITVPFLMKLLKK 191

Query: 196 RNPAAGRFDLKGYLLLTIGMIAISLSLDGLADLGMQHAMVLVLLILSLACFVAYGLYAVR 255
G FD+KG +L+++G++ L L++ +LS FV + +
Sbjct: 192 EVRIKGHFDIKGIILMSVGIVFFMLFTT------SYSISFLIVSVLSFLIFVKHIR---K 242

Query: 256 APQPIFSLELFGIHTFSVGLLGNLFARIGSGAMPYLIPLLLQVSLGYGAFEAG-LMMLPV 314
P L F +G+L ++P +++ E G +++ P
Sbjct: 243 VTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPG 302

Query: 315 AAAGMFSKRIITVLITRHGYRKVLLANTIMVGLMMASFALVSDAMPTWLKIAQLALFGGF 374
+ + I +L+ R G VL + + + + + + ++ I + + GG
Sbjct: 303 TMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGL 362

Query: 375 NSMQFTAMNTLTLKDLGTGGASSGNSLFSLVQMLSMSLGVTVAGALLA 422
+ + T ++T+ L A +G SL + LS G+ + G LL+
Sbjct: 363 SFTK-TVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0953RTXTOXINA280.026 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 28.4 bits (63), Expect = 0.026
Identities = 12/52 (23%), Positives = 26/52 (50%)

Query: 11 AAAIAVVTVTAMAAAPVAAAAAATVTAAAMVAATATAVAVATAVVAATAPAM 62
A+ + TV A ++ ++AAA ++ A + A + + ++ A+ AM
Sbjct: 366 ASLTTISTVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASKQAM 417


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0956TCRTETOQM6280.0 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 628 bits (1620), Expect = 0.0
Identities = 172/683 (25%), Positives = 295/683 (43%), Gaps = 75/683 (10%)

Query: 106 RYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVHDGAATMDWMEQEQERGITITSAAT 165
+ NIG+ AH+DAGKTT TE +L+ +G ++G V G D E++RGITI + T
Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61

Query: 166 TAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGACMVYDSVGGVQPQSETVWR 225
+ W+ ++NIIDTPGH+DF EV RS+ VLDGA ++ + GVQ Q+ ++
Sbjct: 62 SFQWEN-------TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFH 114

Query: 226 QANKYKVPRIAFVNKMDRVGADFFRVQRQIGERLKGVAVPIQIPVGAEEHFQGVVDLVKM 285
K +P I F+NK+D+ G D V + I E+L V Q V M
Sbjct: 115 ALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ----------KVELYPNM 164

Query: 286 KAIVWDDESQGVKFTYEDIPANLVELAHEWREKMVEAAAEASEELLEKYLTDHNSLTEDE 345
+ + Q + E +++LLEKY+ SL E
Sbjct: 165 CVTNFTESEQ------------------------WDTVIEGNDDLLEKYM-SGKSLEALE 199

Query: 346 IKAALRKRTIANEIVPMLCGSAFKNKGVQAMLDAVIDYLPSPADVPAILGHDLDDKEAER 405
++ R + P+ GSA N G+ +++ + + S
Sbjct: 200 LEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH---------------- 243

Query: 406 HPSDDEPFSALAFKIMTDPFVGQLIFFRVYSGVVESGDTLLNATKDKKERLGRILQMHAN 465
FKI +L + R+YSGV+ D++ + K+K ++ +
Sbjct: 244 --RGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYTSING 300

Query: 466 ERKEIKEVRAGDIAAAVG--LK-EATTGDTLCDPGKPIILEKMEFPEPVISQAVEPKTKA 522
E +I + +G+I LK + GDT P + E++E P P++ VEP
Sbjct: 301 ELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQR----ERIENPLPLLQTTVEPSKPQ 356

Query: 523 DQEKMGLALNRLAQEDPSFRVQTDEESGQTIISGMGELHLEIIVDRMKREFGVEATVGKP 582
+E + AL ++ DP R D + + I+S +G++ +E+ ++ ++ VE + +P
Sbjct: 357 QREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEP 416

Query: 583 QVAYRETVRTVAEDVEGKFVKQSGGRGQYGHAVIKLEPNP-GKGYEFLDEIKGGVIPREF 641
V Y E + E + + + + P P G G ++ + G + + F
Sbjct: 417 TVIYME---RPLKKAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF 473

Query: 642 IPAVNKGIEETLKSGVLAGYPVVDVKVHLTFGSYHDVDSNENAFRMAGSMAFKEAMRRAK 701
AV +GI + G L G+ V D K+ +G Y+ S FRM + ++ +++A
Sbjct: 474 QNAVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAG 532

Query: 702 PVLLEPMMAVEVETPEDFMGNVMGDLSSRRGIVQGMEDIAGGGGKLVRAEVPLAEMFGYS 761
LLEP ++ ++ P++++ D + + ++ E+P + Y
Sbjct: 533 TELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ--LKNNEVILSGEIPARCIQEYR 590

Query: 762 TSLRSATQGRATYTMEFKHYAET 784
+ L T GR+ E K Y T
Sbjct: 591 SDLTFFTNGRSVCLTELKGYHVT 613


13BURPS1106A_1033BURPS1106A_1043Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_10330113.139888hypothetical protein
BURPS1106A_10341123.785770hypothetical protein
BURPS1106A_10350113.170658TonB-dependent vitamin B12 receptor BtuB
BURPS1106A_10363134.289183cobalamin ABC transporter permease
BURPS1106A_10371134.249530cobalamin ABC transporter ATP-binding protein
BURPS1106A_10381144.591066cobalamin synthase
BURPS1106A_10390144.535270alpha-ribazole-5'-phosphate phosphatase
BURPS1106A_10400114.149547hypothetical protein
BURPS1106A_1041-1115.515799cobalamin ABC transporter periplasmic
BURPS1106A_1042-1104.967511threonine-phosphate decarboxylase
BURPS1106A_1043-2113.382125cobalamin biosynthesis protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1034BACINVASINB270.015 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 27.4 bits (60), Expect = 0.015
Identities = 22/89 (24%), Positives = 33/89 (37%), Gaps = 4/89 (4%)

Query: 23 QTERLALEEQVAQLRNEAQTLHAELEQLRDERNALAAERDTLSAKIDDAQVKLNAILEKL 82
Q + +E Q+ E QT E ++ D A + DT + D A KL KL
Sbjct: 112 QAMIESQKEMGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAATKKLTQAQNKL 171

Query: 83 ----PRTKNVPDAENQLDLLAPQANDEGE 107
P AE ++ +A + E
Sbjct: 172 QSLDPADPGYAQAEAAVEQAGKEATEAKE 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1035SSBTLNINHBTR300.016 Streptomyces subtilisin inhibitor signature.
		>SSBTLNINHBTR#Streptomyces subtilisin inhibitor signature.

Length = 144

Score = 29.8 bits (66), Expect = 0.016
Identities = 36/109 (33%), Positives = 49/109 (44%), Gaps = 8/109 (7%)

Query: 8 AALAALSGLPCIALAQGDASASSASFASSVS--YAPAAA--SPADADSALSTAPAAAAAS 63
A AA GL A+ A AS AS A++ + YAP+A + +SA + AP A
Sbjct: 5 ARWAATLGLTATAVCGPLAGASLASPATAPASLYAPSALVLTVGHGESAATAAPLRAVTL 64

Query: 64 PASGAARGAEAVSADAASAV--ASGASSASPARAASAAQL--APVVVTA 108
+ A G +A A + + A G SA A + APVVVT
Sbjct: 65 TCAPTASGTHPAAAAACAELRAAHGDPSALAAEDSVMCTREYAPVVVTV 113


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1041FERRIBNDNGPP408e-06 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 39.5 bits (92), Expect = 8e-06
Identities = 39/186 (20%), Positives = 68/186 (36%), Gaps = 9/186 (4%)

Query: 42 AITLAAPARRVVSLAPHVTELIYAAG----GGAKLVGAVSYSDYPPAAKAIARVGSNKAL 97
A A R+V+L EL+ A G G A + + PP ++ VG
Sbjct: 28 AHAAAIDPNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEP 87

Query: 98 DLERIAALKPDLIVVWRHGNAEHETERLRALGIPLYFSEPRH-LDDVAASLDKLGLLLGT 156
+LE + +KP +V E A G FS+ + L SL ++ LL
Sbjct: 88 NLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNL 147

Query: 157 HEIASAAADAYRRRIAQLRARYADK--PPVTVFFQAWDKPLITLNGDH-IVSDVIALCGG 213
A Y I ++ R+ + P+ + D + + G + + +++ G
Sbjct: 148 QSAAETHLAQYEDFIRSMKPRFVKRGARPL-LLTTLIDPRHMLVFGPNSLFQEILDEYGI 206

Query: 214 RNVFAR 219
N +
Sbjct: 207 PNAWQG 212


14BURPS1106A_1114BURPS1106A_1143Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_11145240.038752phosphatidylethanolamine-binding protein
BURPS1106A_111511310.433324hypothetical protein
BURPS1106A_111615341.274302hypothetical protein
BURPS1106A_11171136-0.352124hypothetical protein
BURPS1106A_1118831-0.616955hypothetical protein
BURPS1106A_11206200.189492hypothetical protein
BURPS1106A_1119520-0.186712hypothetical protein
BURPS1106A_11215200.140060lipoprotein
BURPS1106A_1122117-1.251678hypothetical protein
BURPS1106A_1123216-0.264154ecotin
BURPS1106A_1124926-5.563188D-alanyl-D-alanine carboxypeptidase
BURPS1106A_11251030-6.761274hypothetical protein
BURPS1106A_11261030-6.705384hypothetical protein
BURPS1106A_11271029-6.750987hypothetical protein
BURPS1106A_11281030-6.884564hypothetical protein
BURPS1106A_11291030-6.954402cell surface protein
BURPS1106A_1130536-7.170160hypothetical protein
BURPS1106A_1131631-5.494242hypothetical protein
BURPS1106A_1132429-5.722713hemolysin activation/secretion protein
BURPS1106A_1134236-5.717742*integrase catalytic subunit
BURPS1106A_1135230-4.248942hypothetical protein
BURPS1106A_1136227-3.583477hypothetical protein
BURPS1106A_1137226-3.885463hypothetical protein
BURPS1106A_1138227-3.625909hypothetical protein
BURPS1106A_1139014-2.040123translation initiation factor IF-1
BURPS1106A_1140012-1.588595alpha/beta hydrolase
BURPS1106A_1141210-0.805814hypothetical protein
BURPS1106A_1142313-0.347760rubredoxin
BURPS1106A_1143212-0.067524hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1121cloacin280.009 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 28.1 bits (62), Expect = 0.009
Identities = 18/59 (30%), Positives = 22/59 (37%), Gaps = 1/59 (1%)

Query: 49 GTVNVWGGNGWRDRDHWHGGDDRWHGGWRGGGNWRDGNDWHGGRGNGWQGGRGPAGGRN 107
G + G G D W ++ W GG G +W G HG G G G G N
Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW-GGGSGHGNGGGNGNSGGGSGTGGN 80



Score = 26.2 bits (57), Expect = 0.047
Identities = 20/51 (39%), Positives = 23/51 (45%), Gaps = 3/51 (5%)

Query: 74 GGWRGGGNWRDGNDWHGGRGNGWQGGRGPAGGRNVRGGNDWPDGGGNGRGG 124
G G G + N W GG G+G G G G GN GGG+G GG
Sbjct: 32 GASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN---SGGGSGTGG 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1129PF05860677e-15 haemagglutination activity domain.
		>PF05860#haemagglutination activity domain.

Length = 117

Score = 66.8 bits (163), Expect = 7e-15
Identities = 23/138 (16%), Positives = 50/138 (36%), Gaps = 23/138 (16%)

Query: 82 AQVVG-AGANAPSVIQTQNGLQQVNITKPSGAGVSLNTYSQFDVPKQGVIVNNSPTLTNT 140
AQ+ S I T+ + + +G+ + + + +F VP G N+PT
Sbjct: 1 AQITPDTTLPINSNITTEGNTRIIERGTQAGSNLFHS-FQEFSVPTSGTAFFNNPT---- 55

Query: 141 QQAGYINGNPNLGPNGSAKIIINQVNSNNPSQLKGYAEIAGQRAEMIISNPSGLVVDGGG 200
+ + II++V + S + G A + + NP+G++
Sbjct: 56 ----------------NIQNIISRVTGGSVSNIDGLIRANAT-ANLFLINPNGIIFGQNA 98

Query: 201 FINTSRAILTTGTPNLNA 218
++ + + + L
Sbjct: 99 RLDIGGSFVGSTANRLKF 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1132IGASERPTASE320.006 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.3 bits (73), Expect = 0.006
Identities = 14/76 (18%), Positives = 29/76 (38%), Gaps = 2/76 (2%)

Query: 9 PSPADQAAAARANAEQDRQAQQQRDAQQRDAAVRAPSVRSEVPKVEAYPALPAETPCFRI 68
P+PA + AE +Q + + ++DA + ++ EA + A T +
Sbjct: 1028 PAPATPSETTETVAENSKQESKTVEKNEQDAT--ETTAQNREVAKEAKSNVKANTQTNEV 1085

Query: 69 DRFTLDVPNSLPDTTK 84
+ + + TK
Sbjct: 1086 AQSGSETKETQTTETK 1101


15BURPS1106A_1188BURPS1106A_1198Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_11880134.180323hypothetical protein
BURPS1106A_11890134.305469glycosyl hydrolase family protein
BURPS1106A_11902164.917867hypothetical protein
BURPS1106A_11912144.829052hypothetical protein
BURPS1106A_11923155.403324hypothetical protein
BURPS1106A_11933145.345277DNA translocase FtsK
BURPS1106A_11942153.936789hypothetical protein
BURPS1106A_11953184.408039hypothetical protein
BURPS1106A_11961122.887502phosphoribosylglycinamide formyltransferase 2
BURPS1106A_11971152.055915lipoprotein
BURPS1106A_11981113.011961hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1193IGASERPTASE485e-07 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 47.8 bits (113), Expect = 5e-07
Identities = 42/292 (14%), Positives = 80/292 (27%), Gaps = 20/292 (6%)

Query: 379 RAQARPAAPDPRFAPRRPATQAAVSAARNRPMTFTPSRQTTGATPPQPAPRAQTA----- 433
+ R D QA V + + PP PA ++T
Sbjct: 984 EVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARV-DEAPVPPPAPATPSETTETVAE 1042

Query: 434 -----APTAETARKRAPANPARAPLYAWHEKPAERIAPAAS--VHETLRSIEASAAQWTA 486
+ T E + A A+ A K + + + E +
Sbjct: 1043 NSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKE 1102

Query: 487 LAGATSTAATPVTARESMAAPAAPSGGAAASAAPDGHAPTSAETAAPNDHAPTSAETVAP 546
A V ++ P S + + P AE A ND E +
Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQP-QAEPARENDPTVNIKEPQSQ 1161

Query: 547 DGHVPTSAETAAPDSHAPTSAETAAPDSHAPTSAETAAPDGHAPTSAE----TATPNDHA 602
+ + A S T + + ++ P+ P + + + + N
Sbjct: 1162 TNTTADTEQPAKETSSNVEQPVTESTTVNT-GNSVVENPENTTPATTQPTVNSESSNKPK 1220

Query: 603 STSAETAAPDSHAPTSAETAAPDGHASTITEAAAPNGHVSATVETSAVAAPV 654
+ + H A T++ D + + + N + + + A A V
Sbjct: 1221 NRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNTN-AVLSDARAKAQFV 1271



Score = 45.4 bits (107), Expect = 2e-06
Identities = 52/311 (16%), Positives = 96/311 (30%), Gaps = 43/311 (13%)

Query: 558 APDSHAPTSAETAAPDSHAPTSAETAAPDGHAPTSAETATPNDHASTSAETAAPDSHAPT 617
+ P + + P S + E A D ATP++ T AE + +S
Sbjct: 994 TTNITTPNNIQADVP-SVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVE 1052

Query: 618 SAETAA--PDGHASTITEAAAPNGHVSATVETSAVAAPVGITQAAPPIAADTCPAGEHVI 675
E A + + A N V A +T+ VA T+ E
Sbjct: 1053 KNEQDATETTAQNREVAKEAKSN--VKANTQTNEVAQSGSETKETQTTETKETATVE--- 1107

Query: 676 AAVEPAGTSDSAAIGAGAIAHAEAGAAASTAETASPIGVDTHIAPSREADRTAQTAPTAP 735
E A T +T V + ++P +E T Q
Sbjct: 1108 ---------------------KEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPA 1146

Query: 736 SPAEATPHVDAPHALDVAARALVGNTAATAHGAAAVDGSAQRADTASPAASTSGPPAPVA 795
+ T ++ P + NT A A S +G V
Sbjct: 1147 RENDPTVNIKEPQSQT--------NTTADTEQPAKETSSNVEQPVTESTTVNTGN--SVV 1196

Query: 796 ASAASSDRAAPQPVATAAPASIATSGALGTMKAIGAAGPQPSTIAAQRASAIDDTGQPPS 855
+ ++ A QP + ++ + +++++ +P+T ++ S +
Sbjct: 1197 ENPENTTPATTQPTVNSESSNKPKNRHRRSVRSV-PHNVEPATTSSNDRSTV---ALCDL 1252

Query: 856 TGHSTHAAVSN 866
T +T+A +S+
Sbjct: 1253 TSTNTNAVLSD 1263



Score = 39.3 bits (91), Expect = 2e-04
Identities = 47/311 (15%), Positives = 84/311 (27%), Gaps = 39/311 (12%)

Query: 703 ASTAETASPIGVDTHIAPSREADRTA-QTAPTAPSPAEATPHVDAPHALDVAARALVGNT 761
+ T + I D PS + AP P PA ATP +
Sbjct: 994 TTNITTPNNIQADVPSVPSNNEEIARVDEAPVPP-PAPATP----------SETTET-VA 1041

Query: 762 AATAHGAAAVDGSAQRADTASPAASTSGPPAPVAASAASSDRAAPQPVATAAPASIATSG 821
+ + V+ + Q A + VA A S+ +A Q +
Sbjct: 1042 ENSKQESKTVEKNEQDATETTAQNRE------VAKEAKSNVKANTQ-------TNEVAQS 1088

Query: 822 ALGTMKAIGAAGPQPSTIAAQRASAIDDTGQPPSTGHSTHAAVSNELGRRPHAAPDAVTP 881
T + + +T+ + + ++ ++ + E +
Sbjct: 1089 GSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARE 1148

Query: 882 ALPPAAAARAAAVPTSASAVQRQALASESAEAAQGVARAAAAGDSRETTQVSPAGARPDK 941
P + T+ +A Q S+ Q V + + +P P
Sbjct: 1149 NDPTVNIKEPQS-QTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVE-NPENTTPAT 1206

Query: 942 AAPSAAVANPIAPLPGASAITAHEDAPTSAAPDAATPVIAAMDSAMPNAVAPASAIA--S 999
P+ S+ S A S + VA + +
Sbjct: 1207 TQPTVNSE---------SSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVALCDLTSTNT 1257

Query: 1000 NAGMSPASASA 1010
NA +S A A A
Sbjct: 1258 NAVLSDARAKA 1268



Score = 34.7 bits (79), Expect = 0.005
Identities = 37/279 (13%), Positives = 65/279 (23%), Gaps = 33/279 (11%)

Query: 300 PPPASAMPAPTIAAAKPAAATMPPSGLSKAERLAAPTGGAAAPLAAPAAAVTSPAAFAPA 359
PPPA A P+ T + + + T A A A
Sbjct: 1026 PPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNR--EVAKEAKSNVKAN--TQ 1081

Query: 360 ATGIAKPIGSTAAVAALGKRAQARPAAPDPRFAPRRPATQAAVSAARNRPMTFTPSRQTT 419
+A+ T + A + + ++ P
Sbjct: 1082 TNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQP 1141

Query: 420 GATP-PQPAPRAQTAAPTAETARKRAPANPARAPLYAWHEKPAERIAPAASVHETLRSIE 478
A P + P P ++T PA+ ET ++E
Sbjct: 1142 QAEPARENDPTVNIKEPQSQTNTTADTEQPAK---------------------ETSSNVE 1180

Query: 479 ASAAQWTALAGATSTAATPVTARESMAAPAA-------PSGGAAASAAPDGHAPTSAETA 531
+ T + S P + P P S H A T+
Sbjct: 1181 QPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTS 1240

Query: 532 APNDHAPTSAETVAPDGHVPTSAETAAPDSHAPTSAETA 570
+ + + + + + S A A +
Sbjct: 1241 SNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVGKAV 1279


16BURPS1106A_1254BURPS1106A_1266Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1254-1113.693178KDP operon transcriptional regulatory protein
BURPS1106A_12551123.716825hypothetical protein
BURPS1106A_1256-1113.445016hypothetical protein
BURPS1106A_1257-1123.504557SMR family multidrug efflux pump
BURPS1106A_1258-1123.545221hypothetical protein
BURPS1106A_12590124.010129efflux ABC transporter permease
BURPS1106A_12600123.060246hypothetical protein
BURPS1106A_1261-1113.326703hypothetical protein
BURPS1106A_12620113.667422hypothetical protein
BURPS1106A_1263-283.642736threonyl/alanyl tRNA synthetase
BURPS1106A_1264-284.041683hypothetical protein
BURPS1106A_1265-194.174855amidase
BURPS1106A_1266-2103.197566major facilitator family transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1254HTHFIS891e-22 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 89.1 bits (221), Expect = 1e-22
Identities = 39/157 (24%), Positives = 71/157 (45%), Gaps = 3/157 (1%)

Query: 9 TVVLIEDEKQIRRFVRSALEEEGIAVFDAETGRQGLIEAATRKPDLAIVDLGLPDGDGLD 68
T+++ +D+ IR + AL G V A DL + D+ +PD + D
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 69 VIRELR-GWSEMPVIVLSARTHEEEKVAALDAGADDYLTKPFGVSELLARIRAHL--RRR 125
++ ++ ++PV+V+SA+ + A + GA DYL KPF ++EL+ I L +R
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 126 NQAGAAESPVVRFGDVSVDLALRRVWRGGEVVHLTPL 162
+ + V A++ ++R + T L
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDL 161


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1266TCRTETA348e-04 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 34.4 bits (79), Expect = 8e-04
Identities = 30/101 (29%), Positives = 47/101 (46%), Gaps = 9/101 (8%)

Query: 74 ALVIGAYADRAGRKPAMTLTLAMMAVGTGAIAVLPGYETIGVAAPILLVVTRLIQGLAWG 133
A V+GA +DR GR+P + ++LA AV +A P +L + R++ G+ G
Sbjct: 60 APVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLW--------VLYIGRIVAGIT-G 110

Query: 134 GEAGPATTYILEAAPPERRAAYACWQVATQGFAAVAAGLAG 174
A YI + + RA + + A GF VA + G
Sbjct: 111 ATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLG 151


17BURPS1106A_1296BURPS1106A_1311Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1296011-3.359483triosephosphate isomerase
BURPS1106A_1297-113-5.421966preprotein translocase subunit SecG
BURPS1106A_1299012-4.835169*NADH dehydrogenase subunit A
BURPS1106A_1300-113-2.574438NADH dehydrogenase subunit B
BURPS1106A_1301-115-2.921676NADH dehydrogenase subunit C
BURPS1106A_1302-115-2.986144NADH dehydrogenase subunit D
BURPS1106A_1303015-2.466990NADH dehydrogenase subunit E
BURPS1106A_1304016-2.523898NADH-quinone oxidoreductase subunit F
BURPS1106A_1305117-3.227877NADH dehydrogenase subunit G
BURPS1106A_1306118-5.136187NADH dehydrogenase subunit H
BURPS1106A_1307118-4.589385NADH dehydrogenase subunit I
BURPS1106A_1308118-4.548161NADH dehydrogenase subunit J
BURPS1106A_1309018-4.740289NADH dehydrogenase subunit K
BURPS1106A_1310017-4.097690NADH dehydrogenase subunit L
BURPS1106A_1311-314-3.382402NADH dehydrogenase subunit M
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1297SECGEXPORT838e-24 Protein-export SecG membrane protein signature.
		>SECGEXPORT#Protein-export SecG membrane protein signature.

Length = 110

Score = 82.7 bits (204), Expect = 8e-24
Identities = 46/102 (45%), Positives = 68/102 (66%), Gaps = 1/102 (0%)

Query: 8 IIVVQLLSALGVIGLVLLQHGKGADMGAAFGSGASGSLFGATGSANFLSRTTAVLATIFF 67
++VV L+ A+G++GL++LQ GKGADMGA+FG+GAS +LFG++GS NF++R TA+LAT+FF
Sbjct: 5 LLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFF 64

Query: 68 VATLALTYLGSYKSAPSVGVLGAAPAPAASAPAASQTPAASA 109
+ +L L + S K+ APA + PA
Sbjct: 65 IISLVLGNINSNKTNKGSEWEN-LSAPAKTEQTQPAAPAKPT 105


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1306OUTRMMBRANEA300.013 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 29.9 bits (67), Expect = 0.013
Identities = 16/96 (16%), Positives = 28/96 (29%), Gaps = 10/96 (10%)

Query: 138 YAVILAGWASNSKYAFLGAMR-------AAAQMVSYEISMGFALVLVLMTAGSLNLSEIV 190
Y GW+ F+ A Y+++ + G +
Sbjct: 29 YTGAKLGWSQYHDTGFINNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPY---K 85

Query: 191 GSQQHGFFAGHGVNFLSWNWLPLLPVFVIYFISGIA 226
GS ++G + GV + P+ IY G
Sbjct: 86 GSVENGAYKAQGVQLTAKLGYPITDDLDIYTRLGGM 121


18BURPS1106A_1333BURPS1106A_1346Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1333015-3.144484hypothetical protein
BURPS1106A_1334-113-3.614636DNA-binding response regulator
BURPS1106A_1335-113-3.468406hypothetical protein
BURPS1106A_1336115-4.020700hypothetical protein
BURPS1106A_1342115-3.125593**M24 family metallopeptidase
BURPS1106A_1343113-2.582744hypothetical protein
BURPS1106A_1344115-1.744584AraC family transcriptional regulator
BURPS1106A_1345320-0.560805D-3-phosphoglycerate dehydrogenase
BURPS1106A_13465290.401624hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1333OMADHESIN290.021 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

Length = 455

Score = 28.7 bits (63), Expect = 0.021
Identities = 15/45 (33%), Positives = 25/45 (55%)

Query: 150 AIAVGIVAAAAAGVQIAIAEGTLVVVPSGYALNALLLALGEAWFT 194
+IA+G A AA G +A+ G++ + A+ L ALG++ T
Sbjct: 72 SIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVT 116


19BURPS1106A_1355BURPS1106A_1411Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1355210-0.526245cytochrome c
BURPS1106A_1356111-1.413458cytochrome c family protein
BURPS1106A_1357012-1.105797hypothetical protein
BURPS1106A_1358010-0.570715cytochrome c oxidase subunit II
BURPS1106A_1359-110-0.569226cytochrome c oxidase subunit I
BURPS1106A_1360-190.106948cytochrome c family protein
BURPS1106A_1361-270.612888hypothetical protein
BURPS1106A_13620110.603672hypothetical protein
BURPS1106A_13632130.019370sensory box diguanylate cyclase/cyclic
BURPS1106A_13642140.108140antioxidant, AhpC/TSA family protein
BURPS1106A_13652130.403286hypothetical protein
BURPS1106A_13662120.164913hypothetical protein
BURPS1106A_1367113-0.532244multidrug resistance protein MdtC
BURPS1106A_1368011-0.736131multidrug resistance protein MdtB
BURPS1106A_1369212-0.890626membrane fusion protein MdtA
BURPS1106A_1370213-0.642857IclR family transcriptional regulator
BURPS1106A_1371213-0.625149hypothetical protein
BURPS1106A_13721120.365112hypothetical protein
BURPS1106A_13730121.856962hypothetical protein
BURPS1106A_13740142.043900hypothetical protein
BURPS1106A_1375-2123.233195hypothetical protein
BURPS1106A_13760103.903615transcriptional regulator
BURPS1106A_13770113.419609hypothetical protein
BURPS1106A_13780113.323498cysteine dioxygenase, type I
BURPS1106A_1379-1132.555899AsnC family transcriptional regulator
BURPS1106A_13800152.730295iron ABC transporter ATP-binding protein
BURPS1106A_1381-1152.195303iron ABC transporter permease
BURPS1106A_1382-113-0.667505iron ABC transporter substrate-binding protein
BURPS1106A_1383-215-0.849995hypothetical protein
BURPS1106A_1384-1122.491778hypothetical protein
BURPS1106A_13850113.347963amino acid transporter
BURPS1106A_1386094.455790hypothetical protein
BURPS1106A_1387094.456060hypothetical protein
BURPS1106A_1388195.405837hypothetical protein
BURPS1106A_1389185.086467exodeoxyribonuclease V subunit gamma
BURPS1106A_1390194.468699exodeoxyribonuclease V subunit beta
BURPS1106A_13912103.871547exodeoxyribonuclease V subunit alpha
BURPS1106A_1392220-0.234500peptidyl-tRNA hydrolase domain-containing
BURPS1106A_13931120.479730hypothetical protein
BURPS1106A_1394-2110.873513EAL domain-containing protein
BURPS1106A_13953200.438820hypothetical protein
BURPS1106A_1396-212-0.966996hypothetical protein
BURPS1106A_1397-212-1.367392hypothetical protein
BURPS1106A_1398-310-0.074739hypothetical protein
BURPS1106A_1399-190.093985lipoprotein
BURPS1106A_1400-29-0.305248osmotically inducible lipoprotein B
BURPS1106A_1401-29-0.129043thiamine biosynthesis protein ThiC
BURPS1106A_14020101.661285hypothetical protein
BURPS1106A_1403083.048514hypothetical protein
BURPS1106A_1404082.246140hypothetical protein
BURPS1106A_14051112.333202molybdopterin-binding oxidoreductase
BURPS1106A_14062133.575715hypothetical protein
BURPS1106A_14074133.388766integral membrane protein
BURPS1106A_14084133.577437major facilitator family transporter
BURPS1106A_14092142.988109hypothetical protein
BURPS1106A_14101163.233096hypothetical protein
BURPS1106A_14112143.395145hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1365PF01540290.015 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

Length = 475

Score = 28.9 bits (64), Expect = 0.015
Identities = 26/84 (30%), Positives = 38/84 (45%), Gaps = 3/84 (3%)

Query: 13 RTGRALADLLLKQQDFEVTALVRRPDFA--LPGAKVVVADLTGDFSSAFN-GITHAIYAA 69
+ G+ AD LKQ + L + PD++ L +A+ T F A + G AI +
Sbjct: 35 KNGKEKADAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYGDYPAIISK 94

Query: 70 GSAESEGATEEEQIDRDAVARAAD 93
SA E A E+Q A + AD
Sbjct: 95 LSAAVENAKSEQQKVDQANKKIAD 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1367ACRIFLAVINRP7450.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 745 bits (1925), Expect = 0.0
Identities = 279/1104 (25%), Positives = 502/1104 (45%), Gaps = 100/1104 (9%)

Query: 3 LARPFITRPVATTLLALGIALAGLFAFVKLPVSPLPQVDFPTILVQASLPGASPETVATS 62
+A FI RP+ +LA+ + +AG A ++LPV+ P + P + V A+ PGA +TV +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VTSPLERHLGSIADVAEMTSMS-SVGNARIVLQFNLNRDIDGAARDVQAAINAARADLPA 121
VT +E+++ I ++ M+S S S G+ I L F D D A VQ + A LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 SLKSNPTYRKVNPADSPIMVVSLTS--KTASPAKLYDAASTVLQQSLSQIDGIGQVSLSG 179
++ + S +MV S + + D ++ ++ +LS+++G+G V L G
Sbjct: 121 EVQ-QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG 179

Query: 180 SANPAVRVELEPQALFHYGIGLEDVRAALASANANSPKGAIEAGP------HRYQLYTND 233
+ A+R+ L+ L Y + DV L N G + P +
Sbjct: 180 AQY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQT 238

Query: 234 QATKAAQYKDLVI-AYRNHAAVSLSDVSSVVDSVEDLRNLGLMNGERAVLVILYRSPGAN 292
+ ++ + + + + V L DV+ V E+ + +NG+ A + + + GAN
Sbjct: 239 RFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGAN 298

Query: 293 IIDTIERVKAALPQLTAALPADIQVTPVLDRSRTIRASLADTEHTLIIAVSLVVMVVFLF 352
+DT + +KA L +L P ++V D + ++ S+ + TL A+ LV +V++LF
Sbjct: 299 ALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLF 358

Query: 353 LRNWRATLIPSVAVPISIVGTFGAMYLLGFSLNNLSLMALIVATGFVVDDAIVVLENIAR 412
L+N RATLIP++AVP+ ++GTF + G+S+N L++ +++A G +VDDAIVV+EN+ R
Sbjct: 359 LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVER 418

Query: 413 HI-ENGTPRLQAAFDGAREVGFTVLSISLSLVAVFLPILLMGGIVGRLFREFALTLSLAI 471
+ E+ P +A ++ ++ I++ L AVF+P+ GG G ++R+F++T+ A+
Sbjct: 419 VMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAM 478

Query: 472 AVSLVVSLTLTPMMCARLLPEAHAPRDE--GRVARWLERGFEWMQRGYERTLSWALRHPF 529
A+S++V+L LTP +CA LL A E G W F+ Y ++ L
Sbjct: 479 ALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTG 538

Query: 530 TILMTLVATIALNIALYIVVPKGFFPQQDTGLMIGGIQADQTTSFQAMKLRFTEMMRIIR 589
L+ +A + L++ +P F P++D G+ + IQ + + + ++
Sbjct: 539 RYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYL 598

Query: 590 ANP-----NVANVAGFT-GGAQTNSGFMFVALKDKPQR---KLSADQVIQQLRPQLAEVA 640
N +V V GF+ G N+G FV+LK +R + SA+ VI + + +L ++
Sbjct: 599 KNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 641 GARTFLQAAQDIRAGGRQSNAQYQFT-LLGDSTAELYKWGP-ILTEALQKRPELADVNSD 698
I G + ++ G L + +L A Q L V +
Sbjct: 659 DGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 699 QQQGGLEAMVTIDRATAARLGIKPAQIDNTLYDAFGQRQVSTIYNPLNQYHVVMEVAPQY 758
+ + + +D+ A LG+ + I+ T+ A G V+ + + ++ ++
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 759 WQSPEMLKQIYISTSGGSASGVQTTNAAAGTYVATTARASTAGAAAQSAAAIAADSARNQ 818
PE + ++Y+ ++ G V + +V + R R
Sbjct: 779 RMLPEDVDKLYVRSANGEM--VPFSAFTTSHWVYGSPRLE-----------------RYN 819

Query: 819 ALNSIASSG--KSSASSGAAVSTSKSTMVPLSAIASFGPSTTPLAVNHQGLFVATTISFN 876
L S+ G SSG A++ ++
Sbjct: 820 GLPSMEIQGEAAPGTSSGDAMALMENLAS------------------------------K 849

Query: 877 LPPGVSLSKATQVIYQTMAEVGVPPTIQGSFQGTAQAFQESLKDQPILILAALAAVYIVL 936
LP G+ G + P L+ + V++ L
Sbjct: 850 LPAGIGY------------------DWTGMSYQERLSG----NQAPALVAISFVVVFLCL 887

Query: 937 GILYESYIHPVTILSTLPSAGVGALLGLLLFKTEFSIIALIGVILLIGIVKKNAIMMVDF 996
LYES+ PV+++ +P VG LL LF + + ++G++ IG+ KNAI++V+F
Sbjct: 888 AALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEF 947

Query: 997 AIDA-SRQGKSSFDAIHEACLLRFRPIMMTTMAALLGALPLAFGRGDGAEMRAPLGIAIA 1055
A D ++GK +A A +R RPI+MT++A +LG LPLA G G+ + +GI +
Sbjct: 948 AKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVM 1007

Query: 1056 GGLIVSQMLTLYTTPVVYLYMDRL 1079
GG++ + +L ++ PV ++ + R
Sbjct: 1008 GGMVSATLLAIFFVPVFFVVIRRC 1031



Score = 96.1 bits (239), Expect = 4e-22
Identities = 83/503 (16%), Positives = 167/503 (33%), Gaps = 25/503 (4%)

Query: 2 NLARPFITRPVATTLLALGIALAGLFAFVKLPVSPLPQVDFPTILVQASLP-GASPETVA 60
N + L+ I + F++LP S LP+ D L LP GA+ E
Sbjct: 528 NSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQ 587

Query: 61 TSVTSPLERHLGSIAD----VAEMTSMSSVGNAR----IVLQFNLNRDIDGAARDVQAAI 112
+ + +L + V + S G A+ + + +G +A I
Sbjct: 588 KVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVI 647

Query: 113 NAARADLPASLKSNPTYRKVNPADSPIMVVSLTSKT-----ASPAKLYDAASTVLQQSLS 167
+ A+ +L + + L A + +L +
Sbjct: 648 HRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQ 707

Query: 168 QIDGIGQVSLSGSAN-PAVRVELEPQALFHYGIGLEDVRAALASANANSPKGAIEAGPHR 226
+ V +G + ++E++ + G+ L D+ +++A +
Sbjct: 708 HPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRV 767

Query: 227 YQLYT---NDQATKAAQYKDLVIAYRNHAAVSLSDVSSVVDSVEDLRNLGLMNGERAVLV 283
+LY L + N V S ++ V L NG ++ +
Sbjct: 768 KKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHW-VYGSPRLERYNGLPSMEI 826

Query: 284 ILYRSPGANIIDTIERVKAALPQLTAALPADIQVTPVLDRSRTIRASLADTEHTLIIAVS 343
+PG + D A + L + LPA I S R S + I+
Sbjct: 827 QGEAAPGTSSGD----AMALMENLASKLPAGIGYD-WTGMSYQERLSGNQAPALVAISFV 881

Query: 344 LVVMVVFLFLRNWRATLIPSVAVPISIVGTFGAMYLLGFSLNNLSLMALIVATGFVVDDA 403
+V + + +W + + VP+ IVG A L + ++ L+ G +A
Sbjct: 882 VVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNA 941

Query: 404 IVVLENI-ARHIENGTPRLQAAFDGAREVGFTVLSISLSLVAVFLPILLMGGIVGRLFRE 462
I+++E + G ++A R +L SL+ + LP+ + G
Sbjct: 942 ILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNA 1001

Query: 463 FALTLSLAIAVSLVVSLTLTPMM 485
+ + + + ++++ P+
Sbjct: 1002 VGIGVMGGMVSATLLAIFFVPVF 1024



Score = 59.9 bits (145), Expect = 5e-11
Identities = 37/225 (16%), Positives = 84/225 (37%), Gaps = 4/225 (1%)

Query: 870 ATTISFNLPPGVSLSKATQVIYQTMAEV--GVPPTIQGS-FQGTAQAFQESLKDQPILIL 926
A + L G + + I +AE+ P ++ T Q S+ + +
Sbjct: 286 AAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLF 345

Query: 927 AALAAVYIVLGILYESYIHPVTILSTLPSAGVGALLGLLLFKTEFSIIALIGVILLIGIV 986
A+ V++V+ + ++ + +P +G L F + + + G++L IG++
Sbjct: 346 EAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLL 405

Query: 987 KKNAIMMVDFAIDASRQGKSSF-DAIHEACLLRFRPIMMTTMAALLGALPLAFGRGDGAE 1045
+AI++V+ + K +A ++ ++ M +P+AF G
Sbjct: 406 VDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGA 465

Query: 1046 MRAPLGIAIAGGLIVSQMLTLYTTPVVYLYMDRLRVWAEKRRDRR 1090
+ I I + +S ++ L TP + + +
Sbjct: 466 IYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGG 510


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1368ACRIFLAVINRP8010.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 801 bits (2071), Expect = 0.0
Identities = 284/1035 (27%), Positives = 498/1035 (48%), Gaps = 31/1035 (2%)

Query: 4 SRVFILRPVGTALLMAAIMLAGLVALRFLPLAALPEVDYPTIQVQTFYPGASPEVMTSSV 63
+ FI RP+ +L +M+AG +A+ LP+A P + P + V YPGA + + +V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 64 TAPLERQFGQMPSLNQMSSQS-SAGASVITLQFSLDLPLDIAEQEVQAAINAAGNLLPSD 122
T +E+ + +L MSS S SAG+ ITL F DIA+ +VQ + A LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 123 LPAPPIYAKVNPADAPVITLAVTSKTLPLTQ--VQDLADTRLAMKISQVSGVGLVSLSGG 180
+ I + + ++ S TQ + D + + +S+++GVG V L G
Sbjct: 122 VQQQGIS-VEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 NRPAVRIQANPLALASYGLNLDDLRTTISNLNVNTPKGNFDGP------TRAYTINANDQ 234
A+RI + L Y L D+ + N G G +I A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 235 LTSADQYNDAVV-AYKNGRPVMLTDVAKIVAGSENTKLGAWVDAEPAIILNVQRQPGANV 293
+ +++ + +G V L DVA++ G EN + A ++ +PA L ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 IQTVDNVKAILPKLQESLPAALDVQIVTDRTTMIRAAVRDVQFELGLAVALVVLVMYLFL 353
+ T +KA L +LQ P + V D T ++ ++ +V L A+ LV LVMYLFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 ANVYATIIPSLSVPLSLIGTLAVMYLSGFSLNNLSLMALTIATGFVVDDAIVMIENIARY 413
N+ AT+IP+++VP+ L+GT A++ G+S+N L++ + +A G +VDDAIV++EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 -VEEGDSALEAALKGSKQIGFTIISLTVSLIAVLIPLLFMGDVVGRLFHEFAITLAVTIV 472
+E+ EA K QI ++ + + L AV IP+ F G G ++ +F+IT+ +
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 ISAVVSLTLVPMMCAKLLRHTPPPESHRFEAKVHGLIERV----IERYGVALQWVLDRQR 528
+S +V+L L P +CA LL+ E H + G + Y ++ +L
Sbjct: 480 LSVLVALILTPALCATLLK-PVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTG 538

Query: 529 ATLVVAVLTLALTALLYAVIPKGFFPTQDTGVIQAITQAPQSVSYGAMAERQQALAAEIL 588
L++ L +A +L+ +P F P +D GV + Q P + + + L
Sbjct: 539 RYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYL 598

Query: 589 KH--PDVVSLTSFIGVDGANITLNSGRMLINLKPRDERS---ESASDVIRSLQRQVANVT 643
K+ +V S+ + G + N+G ++LKP +ER+ SA VI + ++ +
Sbjct: 599 KNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 644 GISLYMQPVQDLTIDSTVSPTQYQFMLTS---PNPDEFATWVPKLVDRLKKEPS-LADVA 699
+ P I + T + F L D +L+ + P+ L V
Sbjct: 659 DGFVI--PFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVR 716

Query: 700 TDLQNSGKSVYIEIDRTSAARFGITPATVDNALYDAYGQRIVSTIFTQSNQYRVILESEP 759
+ +E+D+ A G++ + ++ + A G V+ + ++ ++++
Sbjct: 717 PNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADA 776

Query: 760 QMQHYTDSLNGIYLPSAGGGQVPLSAIATFRERPAPLLVSHLSQFPATTISFNLAPGASL 819
+ + + ++ +Y+ SA G VP SA T + + P+ I APG S
Sbjct: 777 KFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSS 836

Query: 820 GEAVKAIDAAERELGLPASFQTRFQGAALAFQASLSNQLFLILAAIVTMYIVLGVLYESY 879
G+A+ ++ +L PA + G + + S + L+ + V +++ L LYES+
Sbjct: 837 GDAMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESW 894

Query: 880 IHPITILSTLPSAGVGALLALMITGHDLDIIGIIGIVLLIGIVKKNAIMMIDFALEAERV 939
P++++ +P VG LLA + D+ ++G++ IG+ KNAI++++FA +
Sbjct: 895 SIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEK 954

Query: 940 EGKPPREAIYQACLLRFRPILMTTLAALLGAVPLIVGSGAGSELRQPLGIAIAGGLIVSQ 999
EGK EA A +R RPILMT+LA +LG +PL + +GAGS + +GI + GG++ +
Sbjct: 955 EGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSAT 1014

Query: 1000 VLTLFTTPVIYLGFD 1014
+L +F PV ++
Sbjct: 1015 LLAIFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1369RTXTOXIND484e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 48.3 bits (115), Expect = 4e-08
Identities = 27/149 (18%), Positives = 57/149 (38%), Gaps = 16/149 (10%)

Query: 84 AARGEMPVVLNALGTVTPLANV-TVRTQLSGYLQAVSFQEGQIVKKGDVLAQIDPRP--- 139
+ G++ +V A G +T ++ + ++ + +EG+ V+KGDVL ++
Sbjct: 75 SVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 140 ----YQISLANAQGALARDEALLATARLDLKRYQTLVAQ---DSIAKQTADTQASLVKQY 192
Q SL A+ R + L + L+ L + +++++ SL+K+
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKE- 193

Query: 193 EGTVQIDRAAIDSAKLNLAYARITAPVSG 221
Q + L + A
Sbjct: 194 ----QFSTWQNQKYQKELNLDKKRAERLT 218



Score = 38.3 bits (89), Expect = 5e-05
Identities = 33/182 (18%), Positives = 61/182 (33%), Gaps = 26/182 (14%)

Query: 141 QISLANAQGALARDEALLAT--ARLDLKRYQTLVAQDSIAKQTADTQASLVKQY-EGTVQ 197
+ ++ + L ++L+ + L A++ T + ++ + + T
Sbjct: 251 KHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDN 310

Query: 198 ID--RAAIDSAKLNLAYARITAPVSGRV-GLRQVDPGNYVTPSDT--------NGIVVIT 246
I + + + I APVS +V L+ G VT ++T + + V
Sbjct: 311 IGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTA 370

Query: 247 QLQPMSVIFTTSEDNLPAILKQVGAGGKLSVTAYNRNNTTPLETGV-LDTLDNQIDTATG 305
+Q + F AI+K V A+ L V LD D G
Sbjct: 371 LVQNKDIGFINVG--QNAIIK---------VEAFPYTRYGYLVGKVKNINLDAIEDQRLG 419

Query: 306 TV 307
V
Sbjct: 420 LV 421


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1377NUCEPIMERASE320.001 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 32.4 bits (74), Expect = 0.001
Identities = 21/126 (16%), Positives = 37/126 (29%), Gaps = 30/126 (23%)

Query: 6 LKIALFGATGMIGSRIAAEAARRGHQVTAL-------------SRNPAASGANVQAKAAD 52
+K + GA G IG ++ GHQV + +R + Q D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 53 LFDPASIA--------------AALAGQDVVASAYGPKQEEASKVVAVAKALVDGARKAG 98
L D + V S P S + +++G R
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLA--VRYSLENPHAYADSNLTGFLN-ILEGCRHNK 117

Query: 99 VKRVVV 104
++ ++
Sbjct: 118 IQHLLY 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1401CHLAMIDIAOM6320.007 Chlamydia cysteine-rich outer membrane protein 6 si...
		>CHLAMIDIAOM6#Chlamydia cysteine-rich outer membrane protein 6

signature.
Length = 547

Score = 32.4 bits (73), Expect = 0.007
Identities = 15/61 (24%), Positives = 24/61 (39%), Gaps = 3/61 (4%)

Query: 562 FNLG-LDPDKAREFHDETLPKDSAKVAHFC--SMCGPHFCSMKITQDVREFAAQQGVSEN 618
F LG + P + R E P + + S CG H + +T + E Q ++
Sbjct: 265 FTLGDMQPGEHRTITVEFCPLKRGRATNIATVSYCGGHKNTASVTTVINEPCVQVSIAGA 324

Query: 619 D 619
D
Sbjct: 325 D 325


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1408TCRTETA453e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 44.8 bits (106), Expect = 3e-07
Identities = 60/266 (22%), Positives = 95/266 (35%), Gaps = 11/266 (4%)

Query: 66 YATGMLVLAPLG----DRFDRRTLILLQIAGLSAALVVAAAAPTLGVLAAASLAIGILAT 121
YA AP+ DRF RR ++L+ +AG + + A AP L VL + GI
Sbjct: 52 YALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGA 111

Query: 122 IAQQAVPFAAEIAPPAARGQAVGTVMSGLLLGILLARTAAGFVAEYFGWRAVFAASVAAL 181
A + A+I R + G + + G++ G + + FAA AAL
Sbjct: 112 TGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAA--AAL 169

Query: 182 AALAAVIVA-RLPRSSPTSTLPYGKLLASMWQLVRELRGLR--EASMTGGAIFAAFSAFW 238
L + LP S P + + R RG+ A M I
Sbjct: 170 NGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVP 229

Query: 239 PVLTLLLAGAPFHLGPQAAGL-FGIVGAAGALAAPY-AGRFADKRGPRAIISLAIALIAA 296
L ++ FH G+ G +LA G A + G R + L +
Sbjct: 230 AALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGT 289

Query: 297 SFAIFALSGASLIGLVIGVIVLDVGV 322
+ + A + + I V++ G+
Sbjct: 290 GYILLAFATRGWMAFPIMVLLASGGI 315


20BURPS1106A_1421BURPS1106A_1488Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1421328-4.108374hypothetical protein
BURPS1106A_1422232-5.530267hypothetical protein
BURPS1106A_1423137-7.211590hypothetical protein
BURPS1106A_1424432-5.422634hypothetical protein
BURPS1106A_1425229-3.378208hypothetical protein
BURPS1106A_1426227-2.720221hypothetical protein
BURPS1106A_1427229-2.234425hypothetical protein
BURPS1106A_1428230-2.554123hypothetical protein
BURPS1106A_1429330-3.686203hypothetical protein
BURPS1106A_1430334-4.654576hypothetical protein
BURPS1106A_1431236-6.451158hypothetical protein
BURPS1106A_1432238-7.253874hypothetical protein
BURPS1106A_1433238-7.927193hypothetical protein
BURPS1106A_1434335-7.822962DNA replication protein DnaC
BURPS1106A_1435435-8.156718hypothetical protein
BURPS1106A_1436539-8.618076hypothetical protein
BURPS1106A_1437634-7.336972prophage CP4-57 regulatory protein (AlpA)
BURPS1106A_1438532-5.272037hypothetical protein
BURPS1106A_1439530-4.399321hypothetical protein
BURPS1106A_1440230-3.556151hypothetical protein
BURPS1106A_1441331-2.810501hypothetical protein
BURPS1106A_1442122-1.295888phage integrase
BURPS1106A_14433241.743223hypothetical protein
BURPS1106A_1444115-0.073519hypothetical protein
BURPS1106A_1445016-0.187541hypothetical protein
BURPS1106A_1446-1160.384217hypothetical protein
BURPS1106A_1447-213-0.024471hypothetical protein
BURPS1106A_1448-1120.712638hypothetical protein
BURPS1106A_1449-1110.384879diguanylate cyclase
BURPS1106A_1450-1121.901610hypothetical protein
BURPS1106A_14510103.546618hypothetical protein
BURPS1106A_1452082.984854hypothetical protein
BURPS1106A_14532103.050955hypothetical protein
BURPS1106A_1454493.199305hypothetical protein
BURPS1106A_1455383.573676FMN-binding domain-containing protein
BURPS1106A_1456493.550751GntR family transcriptional regulator
BURPS1106A_1457392.374966AsnC family transcriptional regulator
BURPS1106A_1458183.203926glucosamine--fructose-6-phosphate
BURPS1106A_14591144.120666carotenoid 9,10-9',10' cleavage dioxygenase
BURPS1106A_14601153.924659hypothetical protein
BURPS1106A_14610113.008459LysR family transcriptional regulator
BURPS1106A_14620133.008176short chain dehydrogenase
BURPS1106A_1463-3123.098478lipoprotein
BURPS1106A_1464-1122.164594hypothetical protein
BURPS1106A_14650122.333068hypothetical protein
BURPS1106A_14661121.955031sulfite reductase subunit beta
BURPS1106A_1467-2110.961696hypothetical protein
BURPS1106A_1468-2111.169763GntR family transcriptional regulator
BURPS1106A_14693201.492819hypothetical protein
BURPS1106A_1470-1121.134751hypothetical protein
BURPS1106A_1471-2120.667253HSP20 family protein
BURPS1106A_1472-2161.281924HSP20 family protein
BURPS1106A_1473-3162.049738hypothetical protein
BURPS1106A_1474-3142.321100hypothetical protein
BURPS1106A_1475-2142.329314phosphoenolpyruvate carboxykinase
BURPS1106A_1476-2183.1851213-hydroxyacyl-CoA dehydrogenase
BURPS1106A_14770153.268307hypothetical protein
BURPS1106A_14780132.890884LysR family transcriptional regulator
BURPS1106A_14792143.634703malonate transporter subunit MadL
BURPS1106A_14812144.100627malonate transporter subunit MadM
BURPS1106A_14801154.327437hypothetical protein
BURPS1106A_14821124.970946malonate decarboxylase subunit alpha
BURPS1106A_14833126.518114malonate decarboxylase subunit delta
BURPS1106A_14843126.178732malonate decarboxylase subunit beta
BURPS1106A_14852165.083365malonate decarboxylase subunit gamma
BURPS1106A_14861164.468983phosphoribosyl-dephospho-CoA transferase
BURPS1106A_14872164.551423triphosphoribosyl-dephospho-CoA synthase
BURPS1106A_1488-3113.287441ACP S-malonyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1437HTHFIS250.019 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 25.2 bits (55), Expect = 0.019
Identities = 8/16 (50%), Positives = 12/16 (75%)

Query: 13 AKAAELLGIGVSTLWR 28
KAA+LLG+ +TL +
Sbjct: 453 IKAADLLGLNRNTLRK 468


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1462DHBDHDRGNASE673e-15 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 67.0 bits (163), Expect = 3e-15
Identities = 74/266 (27%), Positives = 119/266 (44%), Gaps = 19/266 (7%)

Query: 1 MADHSIKGKTVIIAGGAKNLGGLIARDLAAQGAQAVAIHYNSAASKGAAAETVAAIEAAG 60
M I+GK I G A+ +G +AR LA+QGA A+ YN + + V++++A
Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLE----KVVSSLKAEA 56

Query: 61 ARAVALQADLTAAGAVEKLFVDTVAAIGRPDIAINTVGKVLKKPFVEITEAEYDEMAAVN 120
A A AD+ + A++++ +G DI +N G + +++ E++ +VN
Sbjct: 57 RHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVN 116

Query: 121 SKTAFFFLKEAGRHVND--NGKIVTLVTSLLGAFTPFYAAYAGMKAPVEHFTRAAAKEFG 178
S F + +++ D +G IVT+ ++ G AAYA KA FT+ E
Sbjct: 117 STGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELA 176

Query: 179 ARGISVTAVGPGPMDTPFFYPAEGADAVAYHKTAAALSPFSKTGL--------TDIGDVV 230
I V PG +T + + A +L F KTG+ +DI D V
Sbjct: 177 EYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETF-KTGIPLKKLAKPSDIADAV 235

Query: 231 PFIRHLVSD-GWWITGQTILINGGYT 255
F LVS IT + ++GG T
Sbjct: 236 LF---LVSGQAGHITMHNLCVDGGAT 258


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1463IGASERPTASE462e-07 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 46.2 bits (109), Expect = 2e-07
Identities = 38/275 (13%), Positives = 73/275 (26%), Gaps = 12/275 (4%)

Query: 200 EPAETAEGAPMKLKTPAAPTPPAAPVPASSAAPGTSASSAVAAPAAAGSGPAASAPAAPV 259
P+ + + A PPA P+ + S + A A
Sbjct: 1007 VPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNR 1066

Query: 260 RHAAPAPASATAAASAPTAASAPAPTPASAPAPASTPAPASAPTPASAPTPTPASAPTPA 319
A A ++ A A + + T + A A T P
Sbjct: 1067 EVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVT 1126

Query: 320 SIPAP----APASAPASTPAPASAPAPASAPAPAPTTNPASSIAPAAAPFASAIPPARAE 375
S +P + P + PA + P + T A + PA ++ P
Sbjct: 1127 SQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTES 1186

Query: 376 KFAPAVTATTAGSASTPASAAAPS----SPSSPSSPWLPPLLPPLLSPDAPSPPADTART 431
+ +T + P+ S + P + + + + + ++ T
Sbjct: 1187 TTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRST 1246

Query: 432 APLAPAAS----PATAAAAATNATATAGAMQSAPR 462
L S + A A ++ +
Sbjct: 1247 VALCDLTSTNTNAVLSDARAKAQFVALNVGKAVSQ 1281



Score = 43.5 bits (102), Expect = 2e-06
Identities = 34/225 (15%), Positives = 58/225 (25%), Gaps = 17/225 (7%)

Query: 178 DPTRRDKAAVKAAEKERVAPLPEPAETAEGAPMKLKTPAAPTPPAAPVPASSAAPGTSAS 237
+ T +++ K A K V + E A+ +T T A V A +
Sbjct: 1060 ETTAQNREVAKEA-KSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEK 1118

Query: 238 SAVAAPAAAGSGPAASAPAAPVRHAAPAPASATAAASAPTAASAPAPTPASAPAPASTPA 297
+ + P A PA + + T A PA
Sbjct: 1119 TQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIK--EPQSQTNTTADTEQPAKE-- 1174

Query: 298 PASAPTPASAPTPTPASAPTPASIPAPAPASAPASTPAPASAPAPASAPAPAPTTNPASS 357
T ++ P S + P +T + P S + P S
Sbjct: 1175 -----TSSNVEQPVTESTTVNTG---NSVVENPENTTPATTQPTVNSESSNKPKNRHRRS 1226

Query: 358 IAPAAA---PFASAIPPARAEKFAPAVTATTAGSASTPASAAAPS 399
+ P ++ + T + + A A A
Sbjct: 1227 VRSVPHNVEPATTSSNDRSTVALCDLTSTNT-NAVLSDARAKAQF 1270


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1475FLGMOTORFLIG340.002 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 33.6 bits (77), Expect = 0.002
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 3/49 (6%)

Query: 571 SPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRL 619
P + + + S P E + +A L D+ S P+ + E + +EK+L
Sbjct: 148 DPQKASFILSSLPTEVQTNVARRIALMDRTS---PEVVREVERVLEKKL 193


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1482ADHESNFAMILY300.018 Adhesin family signature.
		>ADHESNFAMILY#Adhesin family signature.

Length = 309

Score = 30.2 bits (68), Expect = 0.018
Identities = 28/128 (21%), Positives = 42/128 (32%), Gaps = 19/128 (14%)

Query: 390 LKAGEEADARTPAA---LRRGRKLVVQIGE----------TFGEKNAPMFVEQLDALRLA 436
L+ E P A L G I + F EKN + ++LD L
Sbjct: 127 LEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLDKLDKE 186

Query: 437 DKLALDLAPVMVYGDDVTHVVTEEGIANLLMCRDADEREHAIRGVAGYTEIGRGRDRRLV 496
K + P + +VT EG + I + E + + LV
Sbjct: 187 SKDKFNKIP-----AEKKLIVTSEGAFKYF-SKAYGVPSAYIWEINTEEEGTPEQIKTLV 240

Query: 497 ERLRERGV 504
E+LR+ V
Sbjct: 241 EKLRQTKV 248


21BURPS1106A_1542BURPS1106A_1573Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1542-117-3.408674peptidoglycan-binding LysM/M23B peptidase
BURPS1106A_1543224-4.881629aldose 1-epimerase
BURPS1106A_1544530-7.340625UDP pyrophosphate phosphatase
BURPS1106A_1546635-8.766031*prophage DLP12 integrase
BURPS1106A_1547436-8.164789hypothetical protein
BURPS1106A_1548230-5.901617hypothetical protein
BURPS1106A_1549228-4.082851transposase B
BURPS1106A_1550227-4.041027transposase A
BURPS1106A_1551124-1.127372hypothetical protein
BURPS1106A_1552121-0.381166hypothetical protein
BURPS1106A_1553221-0.064437hypothetical protein
BURPS1106A_1554322-1.108951PAAR motif-containing protein
BURPS1106A_1555422-1.655156hypothetical protein
BURPS1106A_1556424-1.067999MerR family transcriptional regulator
BURPS1106A_15573141.864364GntR family transcriptional regulator
BURPS1106A_15584115.071560hypothetical protein
BURPS1106A_15592105.236329hypothetical protein
BURPS1106A_15611105.362547tryptophan repressor binding protein
BURPS1106A_1560095.519417hypothetical protein
BURPS1106A_15620104.822699malto-oligosyltrehalose synthase
BURPS1106A_1563-1114.8107414-alpha-glucanotransferase
BURPS1106A_1564-1113.975372malto-oligosyltrehalose trehalohydrolase
BURPS1106A_1565-1103.870052glycogen debranching protein GlgX
BURPS1106A_1566-183.319870glycogen branching protein
BURPS1106A_1567-182.686373trehalose synthase/ maltokinase
BURPS1106A_15683102.918445glycosyl hydrolase family protein
BURPS1106A_1569928-1.373354hypothetical protein
BURPS1106A_1571926-0.921676hypothetical protein
BURPS1106A_1570726-2.937310poly(3-hydroxybutyrate) depolymerase
BURPS1106A_1572748-4.916153hypothetical protein
BURPS1106A_1573347-3.853119hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1542RTXTOXIND310.003 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.3 bits (71), Expect = 0.003
Identities = 11/64 (17%), Positives = 28/64 (43%), Gaps = 12/64 (18%)

Query: 146 VIAAAAGTVVYAGNGLRGYGNLLIVKHDADFLTTYAHNRALLVKEGQTVAQGQKIAEMGD 205
++A A G + ++G +K + + ++VKEG++V +G + ++
Sbjct: 82 IVATANGKLTHSGR-------SKEIKP---IENSIV--KEIIVKEGESVRKGDVLLKLTA 129

Query: 206 TDND 209
+
Sbjct: 130 LGAE 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1566PRTACTNFAMLY300.031 Pertactin virulence factor family signature.
		>PRTACTNFAMLY#Pertactin virulence factor family signature.

Length = 910

Score = 30.4 bits (68), Expect = 0.031
Identities = 19/63 (30%), Positives = 26/63 (41%), Gaps = 2/63 (3%)

Query: 213 RTEAPPRTASIVADLDALERFGWHDDAWLRARASLDLAHAPVSIYEVHPESWLRVAAEGN 272
+ RT + A L+A RF D +L +A L + A Y + LRV EG
Sbjct: 754 AVKGKYRTHGVGASLEAGRRFTHADGWFLEPQAELAVFRAGGGAYRA--ANGLRVRDEGG 811

Query: 273 RSA 275
S
Sbjct: 812 SSV 814


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1570PF07675310.011 Cleaved Adhesin
		>PF07675#Cleaved Adhesin

Length = 1358

Score = 31.2 bits (70), Expect = 0.011
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 3/46 (6%)

Query: 376 SYNVYRNGNKVGSS-TSTAYTDAGLIAGTAYSYTVTEIDPSLGESA 420
+Y +YRN ++ S T T Y D L G Y+Y V ++ GESA
Sbjct: 1260 TYTIYRNNTQIASGVTETTYRDPDLATGF-YTYGV-KVVYPNGESA 1303


22BURPS1106A_1602BURPS1106A_1639Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1602-193.138607hypothetical protein
BURPS1106A_1603-1131.157443PAAR motif-containing protein
BURPS1106A_1604522-2.811360hypothetical protein
BURPS1106A_1605117-2.074668hypothetical protein
BURPS1106A_1606117-2.128846Rhs element Vgr protein
BURPS1106A_1607325-4.413090hypothetical protein
BURPS1106A_1608427-5.080659hypothetical protein
BURPS1106A_1609327-4.755434hypothetical protein
BURPS1106A_1610-2160.030279Rhs element Vgr protein
BURPS1106A_1611021-0.698061hypothetical protein
BURPS1106A_1612537-6.171317hypothetical protein
BURPS1106A_1613637-6.966765hypothetical protein
BURPS1106A_1614639-7.514000hypothetical protein
BURPS1106A_1615639-7.662025Rhs element Vgr protein
BURPS1106A_16161058-13.404962hypothetical protein
BURPS1106A_16171160-13.435510hypothetical protein
BURPS1106A_1618541-8.673907hypothetical protein
BURPS1106A_1619126-2.454540hypothetical protein
BURPS1106A_1620014-0.915054hypothetical protein
BURPS1106A_1621015-1.093262hypothetical protein
BURPS1106A_1622013-1.015038hypothetical protein
BURPS1106A_1623014-1.125846H-NS histone family protein
BURPS1106A_1624116-1.658286amidohydrolase
BURPS1106A_1625324-3.569283major facilitator family transporter
BURPS1106A_1626938-5.085128hypothetical protein
BURPS1106A_1627432-3.395056LysR family transcriptional regulator
BURPS1106A_1629731-3.333258hypothetical protein
BURPS1106A_1628723-1.494097hypothetical protein
BURPS1106A_16302150.919768hypothetical protein
BURPS1106A_16313112.605050hypothetical protein
BURPS1106A_16324112.347156type I pilus protein
BURPS1106A_16332121.916341type I pilus protein
BURPS1106A_16341132.337692type I pilus protein
BURPS1106A_16351132.020750fimbrial chaperone protein
BURPS1106A_16361112.749564fimbrial usher protein
BURPS1106A_16380121.722887type 1 pili protein CsuE
BURPS1106A_16370121.952513hypothetical protein
BURPS1106A_16392112.532402response regulator/sensor histidine kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1625TCRTETB419e-06 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 40.6 bits (95), Expect = 9e-06
Identities = 59/353 (16%), Positives = 120/353 (33%), Gaps = 55/353 (15%)

Query: 44 VDTQMFSLVIPALLTAWGIGKGQAGLIGGATLAAGAIGGLLAGMIADRFGRVRALQITVC 103
++ + ++ +P + + + A + +IG + G ++D+ G R L +
Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87

Query: 104 WFSLFTFLSAFAQNFEQLLVL-KTLQGLGFGGEWTAGAVLLSETIRARHRGKAMGIVQSA 162
+ + +F LL++ + +QG G V+++ I +RGKA G++ S
Sbjct: 88 INCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSI 147

Query: 163 WGFGWGGAVLLYTLVFSWLPPEWAWRVLFAIGVLPALLVLYIRRAIPEPPRDDAR----- 217
G G + ++ ++ W L I ++ + V ++ + + + R
Sbjct: 148 VAMGEGVGPAIGGMIAHYI----HWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKG 203

Query: 218 ----------------------VAVSTSAAAAQTAPARASAKSIFDPSV------LRMTI 249
+ VS + R DP + + +
Sbjct: 204 IILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVL 263

Query: 250 VGGLIGVGAHGGYHAITTWLPTYLKTERHLSVLGTG------AYLAVIIVAFIIGCMTSA 303
GG+I G + +P +K LS G ++VII +I G
Sbjct: 264 CGGIIFGTVAG----FVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGG----- 314

Query: 304 YLQDRIGRRRNLMLFSACCVVTVNLYVMLPLDNVAMLLLGFPLGFFAAGIPAT 356
L DR G +L ++V+ L + + F G+ T
Sbjct: 315 ILVDRRGPL--YVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFT 365


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1636PF00577456e-150 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 456 bits (1175), Expect = e-150
Identities = 175/865 (20%), Positives = 282/865 (32%), Gaps = 103/865 (11%)

Query: 14 RRRAAAWAVGIAFAAAGHARAGETATLADSF------GRALPPV-------GGAAAHGTL 60
+ R A + V + A A A+A ++ F G GT
Sbjct: 20 KHRLAGFFVRLFVACAFAAQAPLSSA-ELYFNPRFLADDPQAVADLSRFENGQELPPGTY 78

Query: 61 YLELVVN-ALSTGRIVPVRYRDGIYYARA----GDLAQASVRTGAQP-------DALVDL 108
+++ +N R V D LA + T + DA V L
Sbjct: 79 RVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPL 138

Query: 109 -SRLDGVQVEYESAEQRLKLTVPPDWLPRQTLG--SPRLYDRTPAAVSFGLLFNYDVYAN 165
S + + + +QRL LT+P ++ + G P L+D A L NY+ N
Sbjct: 139 TSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINA----GLLNYNFSGN 194

Query: 166 SPT--LGTSYTSAWTEQRLFDRWGTVTNTGVYRRDYGGGAGGVGSNRYLRYDTFWRYSDQ 223
S +G + A+ + G Y GS ++ W D
Sbjct: 195 SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDI 254

Query: 224 DRLR-TYTAGDVITGALSWSSAVRLGGVSVERDFKVRPDIVTYPLPQFSGQAAVPTAVDL 282
LR T GD T + + G + D + PD P G A V +
Sbjct: 255 IPLRSRLTLGDGYTQGDIFDG-INFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTI 313

Query: 283 FINGSKTTTGQVNPGPFTMNNVPFINGAGEATVVTTDALGRQVATTIPFYVANTLLQKGL 342
NG V PGPFT+N++ +G+ V +A G T+P+ L ++G
Sbjct: 314 KQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGH 373

Query: 343 SDYSLSAGAMRRDYGIRSFSYGKFAASGTARHGLTDYLTLEGHVEGGERFALGGLGFDLG 402
+ YS++AG R + T HGL T+ G + +R+ G
Sbjct: 374 TRYSITAGEYRSGNAQQE---KPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKN 430

Query: 403 IGMFGVLGVAATQSRLAGASGRQY---------------------AFGYSYASQRF-SVS 440
+G G L V TQ+ Q+ GY Y++ + + +
Sbjct: 431 MGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFA 490

Query: 441 LQRIQRTNGFRDLS--------VYDLPANVAYRLVRSSTQATGALNLGALG----GTLGA 488
R NG+ + R Q T LG
Sbjct: 491 DTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQ 550

Query: 489 GYFDVRGADGARTRIANLSYTRPLWRRATLYASVNKTVGEHGVAAQLQLIV--PLG---- 542
Y+ D N ++ W TL S+ K + G L L V P
Sbjct: 551 TYWGTSNVDEQFQAGLNTAFEDINW---TLSYSLTKNAWQKGRDQMLALNVNIPFSHWLR 607

Query: 543 -------EPGVVTGALARDANNSFSERVQYSRSVPSDGGLGWNL--AYAGGGSHYQ---- 589
+ +++ D N + ++ D L +++ YAGGG
Sbjct: 608 SDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTG 667

Query: 590 QADATWRNRYFQAQGGVYGYGAGRGYARWGEVQGSVVVMDGAVLPANRVDDAFVLIDTQG 649
A +R Y A G + + V G V+ V ++D VL+ G
Sbjct: 668 YATLNYRGGYGNANIGYSHSDDIKQL--YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPG 725

Query: 650 RGGVPVRYENQLVGKTDGGGHLLVPWAPSYYAGKYEIDPLDLPSNVRVPIVERRVAVRDH 709
V ENQ +TD G+ ++P+A Y + +D L NV + V
Sbjct: 726 AKDAKV--ENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRG 783

Query: 710 GGALVTFPIRRIVCAQIALVDAAGRPVAIGSRVLHEESGETALVGWQGETYLEGLSALNH 769
F R + + +P+ G+ V E S + +V G+ YL G+
Sbjct: 784 AIVRAEFKARVG-IKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGK 842

Query: 770 LRVR--TPDGRTCRATFAADVDAAQ 792
++V+ + C A + ++ Q
Sbjct: 843 VQVKWGEEENAHCVANYQLPPESQQ 867


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1639HTHFIS631e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.3 bits (154), Expect = 1e-12
Identities = 30/122 (24%), Positives = 50/122 (40%), Gaps = 10/122 (8%)

Query: 440 RVLVVDDQEMNRIVLRYQLDALGHHARLCASGDEALRALGTAAYDVVLTDCRMPGMDGIA 499
+LV DD R VL L G+ R+ ++ R + D+V+TD MP +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 500 LTAAIRAH-PDARVRATPIVGVTALVSDAEHARCVDAGMTLCIGKP----TTLDALERAL 554
L I+ PD P++ ++A + + + G + KP + + RAL
Sbjct: 65 LLPRIKKARPD-----LPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 555 VE 556
E
Sbjct: 120 AE 121


23BURPS1106A_1661BURPS1106A_1673Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1661-1123.092954LysR family transcriptional regulator
BURPS1106A_16620142.659733major facilitator superfamily permease
BURPS1106A_16642102.100279peptidase s1, chymotrypsin:pdz/dhr/glgf
BURPS1106A_16632141.551062hypothetical protein
BURPS1106A_16651131.275087hypothetical protein
BURPS1106A_16662103.093095MutT/NUDIX NTP pyrophosphatase
BURPS1106A_16671134.416382hypothetical protein
BURPS1106A_16681134.925918NAD-dependent 4-hydroxybutyrate dehydrogenase
BURPS1106A_16691134.773045hypothetical protein
BURPS1106A_16701144.378679thioesterase family protein
BURPS1106A_16712134.715156branched chain amino acid ABC transporter
BURPS1106A_16721134.499372ABC-transporter
BURPS1106A_16731153.220630ABC transporter ATP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1662FLAGELLIN372e-04 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 36.9 bits (85), Expect = 2e-04
Identities = 41/289 (14%), Positives = 76/289 (26%), Gaps = 14/289 (4%)

Query: 66 WLASANYAGYFVGAMTCARIAVDPARMVRAGLAATVLLTFAMGLASPFWVWALVRFVGGA 125
L+ N VGA I +D ++ L A+ + + + V G
Sbjct: 136 VLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGY 195

Query: 126 VSAWTFVFASQWGLRRVVEHGAPAWGGVIYTGPGIGIVATGLIGFALAGRHAALGWIGFA 185
+ ++ G + T V + A G A
Sbjct: 196 DTY----------AVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANG----QLTTDDA 241

Query: 186 AASAVLTAFVWRAFGAAGAGTDGGQSRGGAKRAGDGVGVADAAGRAGERLAAGATGAAEG 245
+ + F A A + GD + G
Sbjct: 242 ENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVST 301

Query: 246 AEGAEAVADAVSAANVADTADTANSANSANSANSANSANSANSANSANSANSANSANSAN 305
E V V+ A + S+ + ++ + + ++ S AN
Sbjct: 302 TINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEAN 361

Query: 306 SANSANSANSANSATATAERNSPVAGVAGQHGAMAAPAARAPHVESAAA 354
+A S + N A TA +AG+ + A+ + + A
Sbjct: 362 NAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDA 410


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1664V8PROTEASE486e-08 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 47.7 bits (113), Expect = 6e-08
Identities = 32/154 (20%), Positives = 54/154 (35%), Gaps = 26/154 (16%)

Query: 119 GSGFIVGADGIILTTAYVVGQASEATVRLIDRR-----------EFKA-RVLAVDDSSDV 166
SG +VG +LT +VV L F A ++ D+
Sbjct: 104 ASGVVVG-KDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDL 162

Query: 167 AVLQIDATK--------LPTVRLGDSSRVRTGEPVLTIGTPDGSANTVTTGIVSATARML 218
A+++ + + + +++ + + + G P G T + ++
Sbjct: 163 AIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYP-GDKPVATMW--ESKGKIT 219

Query: 219 PDGGRFPFFQTDVTGNLDNSGGPVFNRAGEVIGI 252
G + TG NSG PVFN EVIGI
Sbjct: 220 YLKGEAMQYDLSTTGG--NSGSPVFNEKNEVIGI 251


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1665FLGHOOKAP1280.039 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 27.6 bits (61), Expect = 0.039
Identities = 11/34 (32%), Positives = 16/34 (47%), Gaps = 2/34 (5%)

Query: 190 VDIREEALHELIDRLDDLASEFHSAF--LHEAGK 221
+ R + L + + L LA F AF H+AG
Sbjct: 283 LTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGF 316


24BURPS1106A_1713BURPS1106A_1751Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1713311-2.667859chorismate synthase
BURPS1106A_1714514-3.857130transposase
BURPS1106A_1715414-2.583941electron transfer flavoprotein-ubiquinone
BURPS1106A_1716515-2.609131short chain dehydrogenase
BURPS1106A_1717721-2.964230thioesterase family protein
BURPS1106A_1718518-2.627127hypothetical protein
BURPS1106A_1719318-3.615023hypothetical protein
BURPS1106A_1720214-3.565420hypothetical protein
BURPS1106A_1721-110-3.293666lipoprotein
BURPS1106A_1722-111-0.643730hypothetical protein
BURPS1106A_1723-280.785316sensory box transcriptional regulator
BURPS1106A_1724-2101.5289073-oxoadipate CoA-succinyl transferase subunit
BURPS1106A_1725-1112.3388993-oxoacid CoA-transferase subunit A
BURPS1106A_17261102.7465913-oxoacid CoA-transferase subunit B
BURPS1106A_17270113.059422short chain dehydrogenase
BURPS1106A_1728-291.720887polysaccharide deacetylase
BURPS1106A_1729-310-0.577689hypothetical protein
BURPS1106A_1730-310-1.630896LysR family transcriptional regulator
BURPS1106A_1731-311-2.628441alpha/beta hydrolase
BURPS1106A_1732-112-3.801271endoribonuclease L-PSP
BURPS1106A_1733-111-3.989920GTP diphosphokinase
BURPS1106A_1735-112-4.607087*threonyl-tRNA synthetase
BURPS1106A_1736113-3.905020translation initiation factor IF-3
BURPS1106A_1737013-3.08468750S ribosomal protein L35
BURPS1106A_1738-210-2.60564250S ribosomal protein L20
BURPS1106A_1739-211-3.763598phenylalanyl-tRNA synthetase subunit alpha
BURPS1106A_1740-311-3.417758phenylalanyl-tRNA synthetase subunit beta
BURPS1106A_1741-215-3.686395integration host factor subunit alpha
BURPS1106A_1742-116-3.661444MerR family transcriptional regulator
BURPS1106A_1743-214-3.838506lipoprotein
BURPS1106A_1744023-4.747607acyltransferase
BURPS1106A_1746-115-2.770735*lipoprotein
BURPS1106A_1747020-3.609246hypothetical protein
BURPS1106A_1748020-4.380037hypothetical protein
BURPS1106A_1749-121-4.860870lipoprotein
BURPS1106A_1750233-4.827887antibiotic biosynthesis monooxygenase family
BURPS1106A_1751229-3.823290hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1716DHBDHDRGNASE1205e-35 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 120 bits (301), Expect = 5e-35
Identities = 77/261 (29%), Positives = 125/261 (47%), Gaps = 16/261 (6%)

Query: 7 LEGKVALITGASSGLGQRFAQVLSQAGAKVVLASRRVERLKELRAEIEAAGGAAHVVSLD 66
+EGK+A ITGA+ G+G+ A+ L+ GA + E+L+++ + ++A A D
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65

Query: 67 VTDYQSIRAAVAHAETEAGTIDILVNNSGVSTMQKLVDVSPADFEYVFDTNTRGAFFVAQ 126
V D +I A E E G IDILVN +GV + +S ++E F N+ G F ++
Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125

Query: 127 EVAKRMMMRAGSGNAKPACRIINIASVAGLRPFSQIGLYAMSKAAVVHMTRAMALEWGRH 186
V+K MM R I+ + S P + + YA SKAA V T+ + LE +
Sbjct: 126 SVSKYMMDRRSGS-------IVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEY 178

Query: 187 GINVNAICPGYIDTEINHYLWETEQGQ---------KLQSMLPRRRVGKPQDLDGLLLLL 237
I N + PG +T++ LW E G ++ +P +++ KP D+ +L L
Sbjct: 179 NIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFL 238

Query: 238 AADESQFINGSIVSADDGLGL 258
+ ++ I + D G L
Sbjct: 239 VSGQAGHITMHNLCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1727DHBDHDRGNASE753e-18 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 75.1 bits (184), Expect = 3e-18
Identities = 60/249 (24%), Positives = 99/249 (39%), Gaps = 19/249 (7%)

Query: 10 VLVIGGSSGIGAAAARAFAVLDADVTIASRDANKLAAAARAIDG-PRPVRQAVLDTTDAP 68
+ G + GIG A AR A A + + KL ++ R D D+
Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70

Query: 69 AVDA----FFAEAGPFDHVVMSAAHTPGGPVRKLPLADAQAAMDSKFWGAY----RVARA 120
A+D E GP D +V A G + L + +A G + V++
Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130

Query: 121 ARIAPGGSLTFVSGFLSVRPSASAVLQGAINAALEALARGLALELAP--VRVNTVSPGLV 178
GS+ V + P S + AA + L LELA +R N VSPG
Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGST 190

Query: 179 ATPLWSKL--DDAAREAMYASAAAR----LPARRVGQPEDVANAIVYLAATR--YATGST 230
T + L D+ E + + +P +++ +P D+A+A+++L + + + T
Sbjct: 191 ETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITMHN 250

Query: 231 VLVDGGGAI 239
+ VDGG +
Sbjct: 251 LCVDGGATL 259


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1729PYOCINKILLER280.025 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 27.8 bits (61), Expect = 0.025
Identities = 19/68 (27%), Positives = 27/68 (39%), Gaps = 2/68 (2%)

Query: 22 AATLAPAHADTTGLIEPAHLSVDGSLPAAQRDAQILAARRYDTFWHNGDPALARAALADD 81
AA+LA A +D ++ S + A A + + R W + P R AL D
Sbjct: 274 AASLAQAISDAIAVLGRVLASAPSVM--AVGFASLTYSSRTAEQWQDQTPDSVRYALGMD 331

Query: 82 FADRTPPP 89
A PP
Sbjct: 332 AAKLGLPP 339


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1732SECYTRNLCASE270.020 Preprotein translocase SecY subunit signature.
		>SECYTRNLCASE#Preprotein translocase SecY subunit signature.

Length = 437

Score = 27.0 bits (60), Expect = 0.020
Identities = 10/34 (29%), Positives = 15/34 (44%)

Query: 63 SVQIFISDMANFPGMNEVWDAWVAQGATPPRATV 96
S+ + +A F G N W +WV Q T +
Sbjct: 284 SLLYIPALVAQFAGGNSGWKSWVEQNLTKGDHPI 317


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1741DNABINDINGHU1192e-38 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 119 bits (299), Expect = 2e-38
Identities = 35/89 (39%), Positives = 53/89 (59%)

Query: 37 TKAELAELLFDSVGLNKREAKDMVEAFFEVIRDALENGESVKLSGFGNFQLRDKPQRPGR 96
K +L + ++ L K+++ V+A F + L GE V+L GFGNF++R++ R GR
Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62

Query: 97 NPKTGEAIPIAARRVVTFHASQKLKALVE 125
NP+TGE I I A +V F A + LK V+
Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAVK 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1743PF00577310.025 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 30.6 bits (69), Expect = 0.025
Identities = 32/179 (17%), Positives = 58/179 (32%), Gaps = 36/179 (20%)

Query: 491 APWDAMSDLFNRHLLDYSPRSLNDLKLSADGGALRVRGGIKLWNQVPPGVWLPADMKGSL 550
AP + FN L P+++ DL +G ++PPG + D+ +
Sbjct: 40 APLSSAELYFNPRFLADDPQAVADLSRFENG------------QELPPGTY-RVDIYLNN 86

Query: 551 TLLDERHLAFTPTQVSVLGIP--QAKLLRALGIELSSLAPLKRRGAELRGDSLVLDQYTV 608
+ R + F +P L ++G+ +S++ + + L
Sbjct: 87 GYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLA---DDACVPLTSM-- 141

Query: 609 FPPPVLIGHMSQATVEPDG----LRLTFRPAPNAPVLRPPANLPGSYLWLEGGDTKMFN 663
+ AT + D L LT P A + LW G + + N
Sbjct: 142 ---------IHDATAQLDVGQQRLNLTI---PQAFMSNRARGYIPPELWDPGINAGLLN 188


25BURPS1106A_1774BURPS1106A_1792Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1774-314-3.282908dihydrolipoamide dehydrogenase
BURPS1106A_1775-114-3.476205AFG1 family ATPase
BURPS1106A_1776115-2.662489hypothetical protein
BURPS1106A_17772130.514199hypothetical protein
BURPS1106A_17783152.264235hypothetical protein
BURPS1106A_17794151.791641lipoprotein
BURPS1106A_17814151.964437hypothetical protein
BURPS1106A_17803151.747850hypothetical protein
BURPS1106A_17824202.747144lipoprotein
BURPS1106A_17831142.469331hypothetical protein
BURPS1106A_1784-2130.699148hypothetical protein
BURPS1106A_1785-2112.234467pilin family protein
BURPS1106A_1786-1111.933701peptidase A24A, prepilin type IV
BURPS1106A_17871122.683581TadE family protein
BURPS1106A_17882123.280146pilus assembly protein CpaB
BURPS1106A_17891133.239661type II/III secretion system protein
BURPS1106A_17902123.724738hypothetical protein
BURPS1106A_17911123.377580type II/IV secretion system protein
BURPS1106A_17920113.172530type II secretion system protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1783cloacin457e-07 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 44.7 bits (105), Expect = 7e-07
Identities = 33/117 (28%), Positives = 51/117 (43%), Gaps = 1/117 (0%)

Query: 30 GGSGTISKGLDGSGSGSGGGNAISTTGGSGSGGTSGAGGSGSGGSGSSGSTGGLSGGGGS 89
G+ + S ++G +G G G S G S GGSGSG G +G +GGG
Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-IHWGGGSGHGNGGGNG 69

Query: 90 TSGGGSTSGGGSTSGGTSTSSSINALGTIAGNTGGIISGAGSTVSGLGTVVGSQTLP 146
SGGGS +GG ++ + AL T + AG+ + + ++ + P
Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKGP 126



Score = 40.5 bits (94), Expect = 2e-05
Identities = 33/123 (26%), Positives = 46/123 (37%), Gaps = 2/123 (1%)

Query: 38 GLDGSGSGSGGGNAI-STTGGSGSGGTSGAGGSGSGGSGSSGSTGGLSGGG-GSTSGGGS 95
G DG G +G + + GG G G GSG S + GG SG G G G
Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62

Query: 96 TSGGGSTSGGTSTSSSINALGTIAGNTGGIISGAGSTVSGLGTVVGSQTLPGVNPQTTQA 155
+GGG+ + G + + N A G + + GL + + L A
Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122

Query: 156 LGG 158
L G
Sbjct: 123 LKG 125



Score = 34.7 bits (79), Expect = 0.001
Identities = 34/125 (27%), Positives = 52/125 (41%), Gaps = 4/125 (3%)

Query: 55 TGGSGSGGTSGAGGSGSGGSGSSGSTGGLSGGGGSTSGGGSTSGGGSTSGGTSTSSSINA 114
+GG G G +GA S +G GL GGG++ G G +S GG+ +
Sbjct: 2 SGGDGRGHNTGA---HSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58

Query: 115 LGTIAGNTGGIISGAGSTVSGLGTVVGSQTLPGVNPQTTQALGGVVQSL-GGAVSALGSG 173
G SG GS G + V + G +T GG+ S+ GA+SA +
Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118

Query: 174 VTSGI 178
+ + +
Sbjct: 119 IMAAL 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1786PREPILNPTASE534e-11 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 53.3 bits (128), Expect = 4e-11
Identities = 31/124 (25%), Positives = 52/124 (41%), Gaps = 10/124 (8%)

Query: 4 LFSIGFFFAWAAAVAIADCRDRRIPNELVLVGLAAVIIFTVCRQNPFGTTLSGALIGGAV 63
+ A+ D +P++L L L ++F + F +L A+IG
Sbjct: 134 TLAALLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNL--LGGF-VSLGDAVIGAMA 190

Query: 64 GLVSLFPFFAL-------RVMGAADVKVFAVLGAWCGLSALPRLWVVASVAAGVHALALM 116
G + L+ + MG D K+ A LGAW G ALP + +++S+ + L+
Sbjct: 191 GYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMGIGLI 250

Query: 117 LLTR 120
LL
Sbjct: 251 LLRN 254


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1789BCTERIALGSPD1382e-37 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 138 bits (348), Expect = 2e-37
Identities = 58/249 (23%), Positives = 111/249 (44%), Gaps = 11/249 (4%)

Query: 151 VQVDVRVVEFSRSVLKQAGLNFFKQNNGFTFGSFAPAGLASVTGGG----TSSMSVSANI 206
V V+ + E + G+ + +N G T + + +++ G S+
Sbjct: 347 VLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLA 406

Query: 207 PIASAFN-LVVGSATRGLFADLSILEANNLARVLAQPTLVALSGQSASFLAGGEIPVPVP 265
S+FN + G L+ L ++ +LA P++V L A+F G E+PV
Sbjct: 407 SALSSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTG 466

Query: 266 QSLGT-----ISIDWKPYGVGLTLTPTVLSPRRIALKVAPESSQLDFVHSITINGVTVPA 320
+ +++ K G+ L + P + + L++ E S + S + +
Sbjct: 467 SQTTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAAS-STSSDLGAT 525

Query: 321 LTTRRADTTVELGDGESFAIGGLIDRETTSNVDKVPFLGDLPIIGTFFKHLSYQQNDKEL 380
TR + V +G GE+ +GGL+D+ + DKVP LGD+P+IG F+ S + + + L
Sbjct: 526 FNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNL 585

Query: 381 VIIVTPHLV 389
++ + P ++
Sbjct: 586 MLFIRPTVI 594


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1790HTHFIS385e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 38.3 bits (89), Expect = 5e-05
Identities = 11/63 (17%), Positives = 26/63 (41%)

Query: 79 AALRVSHPGLPIVALGSLGEPESALAALRAGVRDFIDFSAPAEDALRITRGLLDHVGDQP 138
++ + P LP++ + + +A+ A G D++ + + I L +P
Sbjct: 67 PRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRP 126

Query: 139 SRH 141
S+
Sbjct: 127 SKL 129


26BURPS1106A_1919BURPS1106A_1974Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_19193141.589963AraC family transcriptional regulator
BURPS1106A_19203152.512406carbohydrate ABC transporter periplasmic
BURPS1106A_19214153.176951carbohydrate ABC transporter ATP-binding
BURPS1106A_19224153.956285carbohydrate ABC transporter permease
BURPS1106A_19235144.058893hypothetical protein
BURPS1106A_19246164.190845zinc-binding dehydrogenase family
BURPS1106A_19255175.035770carbohydrate kinase
BURPS1106A_19264215.128130short chain dehydrogenase
BURPS1106A_19275225.184865hypothetical protein
BURPS1106A_19291143.864009hypothetical protein
BURPS1106A_19280144.233038hypothetical protein
BURPS1106A_19301146.196965hypothetical protein
BURPS1106A_19312136.209187extracytoplasmic-function sigma-70 factor
BURPS1106A_19323146.626571mbtH domain-containing protein
BURPS1106A_19334126.255061syringomycin biosynthesis enzyme
BURPS1106A_19345126.979637iron ABC transporter ATP-binding protein
BURPS1106A_19351125.888130iron-hydroxamate transporter permease subunit
BURPS1106A_19361136.419655ferric iron reductase
BURPS1106A_19372146.799817iron ABC transporter substrate-binding protein
BURPS1106A_19382146.479494hypothetical protein
BURPS1106A_19391155.870194hypothetical protein
BURPS1106A_19402155.609560cyclic peptide ABC transporter ATP-binding
BURPS1106A_19422155.986429siderophore non-ribosomal peptide synthetase
BURPS1106A_19431124.715853siderophore non-ribosomal peptide synthetase
BURPS1106A_1944-1122.209218L-ornithine 5-monooxygenase MbaA
BURPS1106A_1945-1112.641334ferric malleobactin transporter
BURPS1106A_19462124.445755hypothetical protein
BURPS1106A_19472103.598330hypothetical protein
BURPS1106A_19483122.877960cobyrinic acid a,c-diamide synthase
BURPS1106A_19491122.915188cob(I)yrinic acid a,c-diamide
BURPS1106A_19502113.643365cobalamin biosynthesis protein CbiG
BURPS1106A_19510103.826828hypothetical protein
BURPS1106A_19520104.215185high-affinity nickel transport protein
BURPS1106A_19530104.785088cobalamin biosynthesis protein CobW
BURPS1106A_19540115.220496cobaltochelatase subunit CobN
BURPS1106A_19553125.705114hypothetical protein
BURPS1106A_19561145.438925magnesium chelatase subunit ChII
BURPS1106A_19570133.289045protoporphyrin IX magnesium chelatase
BURPS1106A_19582141.341073phospholipase/carboxylesterase
BURPS1106A_19593124.460722hypothetical protein
BURPS1106A_19602123.840760hypothetical protein
BURPS1106A_19611134.345690hypothetical protein
BURPS1106A_19621145.316266glycosyl hydrolase family protein
BURPS1106A_19631146.343198hypothetical protein
BURPS1106A_19642137.229233cobalamin biosynthesis protein CbiG/precorrin-3B
BURPS1106A_19650146.437887precorrin-2 C(20)-methyltransferase
BURPS1106A_19661167.666156precorrin-8X methylmutase
BURPS1106A_19672157.254283precorrin-3B synthase
BURPS1106A_19682145.340781hypothetical protein
BURPS1106A_19692135.791816precorrin-6Y C5,15-methyltransferase
BURPS1106A_19701144.987511cobalt-precorrin-6A synthase
BURPS1106A_19710125.085214cobalt-precorrin-6x reductase
BURPS1106A_19720103.407863precorrin-4 C(11)-methyltransferase
BURPS1106A_1973-1103.510066lipoprotein
BURPS1106A_1974-2114.109694major facilitator family transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1926DHBDHDRGNASE1232e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 123 bits (310), Expect = 2e-36
Identities = 81/252 (32%), Positives = 118/252 (46%), Gaps = 15/252 (5%)

Query: 9 GRSFLVTGASSGIGRAAAVALRGCGARVVAAARNARELERLAHETGC-----EPLELDVG 63
G+ +TGA+ GIG A A L GA + A N +LE++ E DV
Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 64 CDASVRAALSS-ERMRDAFDGLINCAGVTSLAAAIDTTADEFDRVMAVNARGAMLVARHV 122
A++ + ER D L+N AGV + +E++ +VN+ G +R V
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 123 ARAMIRAGRGGSIVNVSSQAALVALPSHLAYCASKAALDAMTRVLCVELGPHGIRVNSVN 182
++ M R GSIV V S A V S AY +SKAA T+ L +EL + IR N V+
Sbjct: 128 SKYM-MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVS 186

Query: 183 PTVTLTPMAERAWSDPHASGPMLA--------AIPLGRFARVADVVAPILFLSSDAAAMV 234
P T T M W+D + + ++ IPL + A+ +D+ +LFL S A +
Sbjct: 187 PGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHI 246

Query: 235 SGVALPVDGGYT 246
+ L VDGG T
Sbjct: 247 TMHNLCVDGGAT 258


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1934PF05272280.042 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.042
Identities = 12/23 (52%), Positives = 13/23 (56%)

Query: 36 VTALCGPNGCGKSTLLRTLAGLQ 58
L G G GKSTL+ TL GL
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_19362FE2SRDCTASE576e-12 Ferric iron reductase signature.
		>2FE2SRDCTASE#Ferric iron reductase signature.

Length = 262

Score = 57.4 bits (138), Expect = 6e-12
Identities = 51/186 (27%), Positives = 73/186 (39%), Gaps = 24/186 (12%)

Query: 78 RALVSQWSKYYFNLAASAGFAAALLLGRPLDMAPQRMRVALRGGMPVALLFEADALRPAQ 137
+ L+S W+++Y L A L + LD++P+ VA F D
Sbjct: 89 KPLISLWAQWYIGLMVPPLMLALLTQEKALDVSPEHFHAEFHETGRVA-CFWVDVCEDKN 147

Query: 138 AEPAS---RYAALVDH-LRATIDTLAALAKLSPRVLWANAGNLLD-YLFEQCAHAPRAGA 192
A P S R L+ L + L A +++ +++W+N G L++ YL E G
Sbjct: 148 ATPHSPQHRMETLISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEM---KQLLGE 204

Query: 193 DA------AWLFGPVDSRGEANPLRLPVRRVKPCSARLPDPFRARRVCCLRNEIPGEDQL 246
A F + GE NPL V L D RR CC R +P Q
Sbjct: 205 ATVESLRHALFFEKTLTNGEDNPLWRTV--------VLRDGLLVRRTCCQRYRLPDVQQ- 255

Query: 247 CGSCPL 252
CG C L
Sbjct: 256 CGDCTL 261


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1937FERRIBNDNGPP1142e-31 Ferrichrome-binding periplasmic protein signature.
		>FERRIBNDNGPP#Ferrichrome-binding periplasmic protein signature.

Length = 296

Score = 114 bits (287), Expect = 2e-31
Identities = 78/264 (29%), Positives = 113/264 (42%), Gaps = 15/264 (5%)

Query: 115 PARIVVLEFMFAEDLAALDITPVGMADPAYYPIWIGYDDARFARVSDVGTRQEPSLEAIA 174
P RIV LE++ E L AL I P G+AD Y +W+ + V DVG R EP+LE +
Sbjct: 35 PNRIVALEWLPVELLLALGIVPYGVADTINYRLWVS-EPPLPDSVIDVGLRTEPNLELLT 93

Query: 175 AAKPDLILGVGLRHAPIFDALSRIAPTVLFKYSPNYIEDGRQVTQYDWARAILRTIGCLT 234
KP ++ + P + L+RIAP F +S DG+Q AR L + L
Sbjct: 94 EMKPSFMVW-SAGYGPSPEMLARIAPGRGFNFS-----DGKQ--PLAMARKSLTEMADLL 145

Query: 235 GRARDARAVQARVDAGLARDARRIAAAGRAGERVAWLQELGLPDRYWAFTGNSASAGIAR 294
A A+ + + R + G R L L P F NS I
Sbjct: 146 NLQSAAETHLAQYEDFIRSMKPRFV---KRGARPLLLTTLIDPRHMLVFGPNSLFQEILD 202

Query: 295 ALGLE-PWPGEPTREGTAYVTSEDLLKQPDLAVLFVSATEPGVPLDAKLDSSIWRFVPAR 353
G+ W GE G+ V+ + L D+ VL +DA + + +W+ +P
Sbjct: 203 EYGIPNAWQGETNFWGSTAVSIDRLAAYKDVDVLCFDHDNSKD-MDALMATPLWQAMPFV 261

Query: 354 RAGRVALVERNIWGFGGPMSALRL 377
RAGR V +W +G +SA+
Sbjct: 262 RAGRFQRVP-AVWFYGATLSAMHF 284


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1956HTHFIS446e-07 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 44.4 bits (105), Expect = 6e-07
Identities = 39/176 (22%), Positives = 64/176 (36%), Gaps = 14/176 (7%)

Query: 35 DRALPAAYPFSALIGQ-AALQQALLLVA-VDPGLGGVLVSGPRGTAKSTAARALAELLP- 91
+ + L+G+ AA+Q+ ++A + ++++G GT K ARAL +
Sbjct: 127 SKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKR 186

Query: 92 -EGRFVTLPLSASDEQVTGSLDLASALADNT--VRFSPGLVARAHLGVLYVDEINLLPDA 148
G FV + ++A + S T S G +A G L++DEI +P
Sbjct: 187 RNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMD 246

Query: 149 LVDALLDAAASGVNTVERDGVSHSHAARFALVGTMNP------EEGELRPQLLDRF 198
LL G G + +V N +G R L R
Sbjct: 247 AQTRLLRVLQQG--EYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1969OMADHESIN290.027 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

Length = 455

Score = 29.5 bits (65), Expect = 0.027
Identities = 25/63 (39%), Positives = 28/63 (44%)

Query: 147 ADGATPAAIAGALVARGFGPSAMSVFEHLGGPLERRLDARADAWRDARAAALNVVAIECR 206
A GAT A GA VA G G A V GPL + L A + A A + VAI R
Sbjct: 74 AIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDGVAIGAR 133

Query: 207 ACA 209
A
Sbjct: 134 AST 136


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1972LCRVANTIGEN300.008 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 30.0 bits (67), Expect = 0.008
Identities = 16/58 (27%), Positives = 24/58 (41%), Gaps = 5/58 (8%)

Query: 46 RAELVVNTAELDLDEIVALLARAHGKGQDVARVHSG-----DPSLYGAIGEQIRRLAA 98
R EL TAEL + ++ H +H D +LYG E+I + +A
Sbjct: 154 REELAELTAELKIYSVIQAEINKHLSSSGTINIHDKSINLMDKNLYGYTDEEIFKASA 211


27BURPS1106A_1989BURPS1106A_2036Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_19893154.566150hypothetical protein
BURPS1106A_19902144.191614hypothetical protein
BURPS1106A_19912144.729759hypothetical protein
BURPS1106A_19920143.921193amine ABC transporter permease
BURPS1106A_19930124.357239amine ABC transporter ATP-binding protein
BURPS1106A_1994-1134.292611amine ABC transporter permease
BURPS1106A_1995-2133.183918amine ABC transporter periplasmic amine-binding
BURPS1106A_1996-1133.654427hypothetical protein
BURPS1106A_1997-1123.265526methyltransferase UbiE
BURPS1106A_1998-1113.112316major facilitator family transporter
BURPS1106A_1999-1112.256614AMP-binding protein
BURPS1106A_2000-1121.023971hypothetical protein
BURPS1106A_2001-2102.315367hypothetical protein
BURPS1106A_2002-1102.823338methyl-accepting chemotaxis protein
BURPS1106A_20032133.727654chemotaxis protein CheW
BURPS1106A_20040133.232138hypothetical protein
BURPS1106A_20050133.173112hypothetical protein
BURPS1106A_20060142.680976mtultidrug ABC transporter permease
BURPS1106A_20071160.406035AraC family transcriptional regulator
BURPS1106A_2008117-0.862088oxidoreductase
BURPS1106A_2009014-2.197736outer membrane porin
BURPS1106A_2011-215-3.312699hypothetical protein
BURPS1106A_2010-311-3.415833non-ribosomal peptide synthase
BURPS1106A_2012-17-4.312530JmjC domain-containing protein
BURPS1106A_2013-18-3.296007histidinol-phosphate aminotransferase HisC
BURPS1106A_2014011-3.405866hypothetical protein
BURPS1106A_2015012-1.985335formyltransferase
BURPS1106A_2016-113-0.725433argininosuccinate synthase
BURPS1106A_2017-1131.330123argininosuccinate lyase
BURPS1106A_2018-1121.833813GHMP kinase ATP-binding subunit
BURPS1106A_2019-3111.955770major facilitator family transporter
BURPS1106A_2020-1124.109357hypothetical protein
BURPS1106A_2021-1114.076643cysteine synthase
BURPS1106A_2022-1123.707366argininosuccinate lyase
BURPS1106A_2023-1113.758181L-allo-threonine aldolase
BURPS1106A_2024-2113.715448acetyltransferase
BURPS1106A_2025-2103.516041syringomycin synthetase
BURPS1106A_2026-1100.114135hypothetical protein
BURPS1106A_2027-1100.179540carbamoyltransferase family protein
BURPS1106A_20281160.844982penicillin amidase
BURPS1106A_2029325-1.870131hypothetical protein
BURPS1106A_2030324-2.333179hypothetical protein
BURPS1106A_2031423-1.600399hypothetical protein
BURPS1106A_2032425-2.134856hypothetical protein
BURPS1106A_2033428-2.499149hypothetical protein
BURPS1106A_2034322-1.357174hypothetical protein
BURPS1106A_2035117-1.060460RNA polymerase sigma factor
BURPS1106A_2036216-0.810103hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1998TCRTETB901e-21 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 90.3 bits (224), Expect = 1e-21
Identities = 74/403 (18%), Positives = 152/403 (37%), Gaps = 16/403 (3%)

Query: 18 FMQNLDSTVVATALPSMARELGVNVVFLSSAITSYLVALTVFIPVSGWIAEHFGAKRVFI 77
F L+ V+ +LP +A + + T++++ ++ V G +++ G KR+ +
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 78 AAIAIFTAASVMCAAANGLAT-LVAARILQGAGGALMVPVGRLILYRGVSRHEMLAATTW 136
I I SV+ + + L+ AR +QGAG A + +++ R + + A
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 137 LTMPALVGPLLGPPLGGFLTDALSWRAVFWINVPVGVAGAALAARLVPASAGERRAPADA 196
+ +G +GP +GG + + W + + +P+ + + D
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKEVRIKGHFDI 201

Query: 197 RGMLLVGAALAALMLGVETAGRDVLPAGAPALCLGAGVALGGLAIRHCRRVAHPAVDLSL 256
+G++L+ + ML + L + + ++H R+V P VD L
Sbjct: 202 KGIILMSVGIVFFMLFTTSYSISFLIVSVLSF---------LIFVKHIRKVTDPFVDPGL 252

Query: 257 L-GIPTFHAATIAGSLFRAGAGALPFLVPLTLQVGFGASASRSGAITLASA-LGSLVMRP 314
IP G +F AG + +VP ++ S + G++ + + ++
Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFV-SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGY 311

Query: 315 MTHAALHRAPMRTVLIAGSVSFAAVLAACATLSPAWPDAAVFALLLVGGLSRSLSFASLG 374
+ + R VL G + + L ++ V G S + +
Sbjct: 312 IGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSFTKTVIS 370

Query: 375 ALVFSDVPSERLSAATSFQGTAQQLMRAVGVAVAAGALHLAML 417
+V S + + A S L G+A+ G L + +L
Sbjct: 371 TIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLL 413


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2002IGASERPTASE300.024 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.4 bits (68), Expect = 0.024
Identities = 24/171 (14%), Positives = 47/171 (27%), Gaps = 5/171 (2%)

Query: 404 ASEVRSLAQRSSSAAKEIKDLINASVQKIHDGSALAGEAGKTMTEVTQAVARVTDIMGEI 463
+ + S++ D S + + ++ V + E
Sbjct: 1002 NIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATET 1061

Query: 464 AAASGEQSRGIEQVNQAIAQMDEVTQQNAALVEEAAAASKSLEEQGRHLTQAVSFFRASA 523
A + E ++ + +A Q +EV Q + E +K + +
Sbjct: 1062 TAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKA-----KVET 1116

Query: 524 ASAAPQARHAAPAKPKAKRGVAAPASAPRAAHAAPTFNKPAPALAAAATAS 574
+ + PK ++ A A PT N P TA
Sbjct: 1117 EKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTAD 1167


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2009ECOLNEIPORIN942e-23 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 93.7 bits (233), Expect = 2e-23
Identities = 90/380 (23%), Positives = 145/380 (38%), Gaps = 64/380 (16%)

Query: 32 ASTAHAQSSVVLYGLIDTSITYANNQRTHGAGSPGSPGWAVTSGALNASRWGLRGREDLG 91
A A + V LYG I + + + +GA + T S+ G +G+EDLG
Sbjct: 12 ALPVAAMADVTLYGTIKAGVETSRSVAHNGAQAASVE--TGTGIVDLGSKIGFKGQEDLG 69

Query: 92 DGVSAIFALENGFSGASGALSQKGVDMFGRQAWIGLKSKEGGALTLGRQYDLILDF--VT 149
+G+ AI+ +E AS A + G RQ++IGLK G L +GR ++ D +
Sbjct: 70 NGLKAIWQVE---QKASIAGTDSG--WGNRQSFIGLK-GGFGKLRVGRLNSVLKDTGDIN 123

Query: 150 PLGASGPGWGGNLAVHPYDNDDSNRNIRINNAVKYTSPTYRGWTLGAMYGFSNTAGPFGN 209
P + G N P R I +V+Y SP + G + Y ++ AG N
Sbjct: 124 PWDSKSDYLGVNKIAEP-----EARLI----SVRYDSPEFAGLSGSVQYALNDNAG-RHN 173

Query: 210 NAAWSAGLSYANGPLKLGAGYLRINRNPNAANANGALSTTDGSATITGGSQQIWAVAGRY 269
+ ++ AG +Y NG + G + QI + Y
Sbjct: 174 SESYHAGFNYKNGGFFVQYGGAYKRH-------------HQVQENVNIEKYQIHRLVSGY 220

Query: 270 -AFGPHSIGAAWSHSATDRVSGVLQGGSIAKLDGNSLVFDNFTLDGRY-VVTPRLSLAAA 327
++ A A L + + + TL R+ VTPR+S A
Sbjct: 221 DNDALYASVAVQQQDAK------LVEENYSHNSQTEVA---ATLAYRFGNVTPRVSYAHG 271

Query: 328 YTYTMGRFDARSGETRPKWNHMVAQADYAFSIRTDAYLEAVYQRVSGGNGIPAFNATIWT 387
+ + + + ++ +V A+Y FS RT A + A + + G G F +T
Sbjct: 272 FKGSFDATNYNN-----DYDQVVVGAEYDFSKRTSALVSAGWLQ--EGKGESKFVSTA-- 322

Query: 388 LTPSANGNQVVVALGLRHRF 407
+GLRH+F
Sbjct: 323 -----------GGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2021ARGDEIMINASE290.041 Bacterial arginine deiminase signature.
		>ARGDEIMINASE#Bacterial arginine deiminase signature.

Length = 409

Score = 28.6 bits (64), Expect = 0.041
Identities = 11/48 (22%), Positives = 18/48 (37%), Gaps = 2/48 (4%)

Query: 267 PTSGAAFMVAEWLRAQRDDGRTIVFIAPDEGHRYADTVYDDAWLRGQG 314
+G + R Q +DG ++ IAP E Y+ + G
Sbjct: 334 KCAGGDLIHGA--REQWNDGANVLAIAPGEIIAYSRNHVTNKLFEENG 379


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2035PF08280280.020 M protein trans-acting positive regulator
		>PF08280#M protein trans-acting positive regulator

Length = 530

Score = 28.3 bits (63), Expect = 0.020
Identities = 23/95 (24%), Positives = 39/95 (41%), Gaps = 17/95 (17%)

Query: 21 RSFLSELTRHLRG--FLRKRIPQFDADIEDLVQEILLAVHNARHTYRADEPLTAWVHAIA 78
SFLS + HL+ +L + +D ILLA+ RH + P T +
Sbjct: 209 HSFLSHSSTHLKTSPWLSESFSFYD---------ILLALSWKRHQFSVTIPQTRIFQQLK 259

Query: 79 RYKLMDFFRTRARREALHDPLDDHTDI-FSEPDDD 112
+ + D + +R D ++ + + FS D D
Sbjct: 260 KLFVYDSLKKSSR-----DIIETYCQLNFSAGDLD 289


28BURPS1106A_2046BURPS1106A_2117Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2046425-6.244282Is2000 transposase
BURPS1106A_2047527-6.759101hypothetical protein
BURPS1106A_2048529-9.000927outer membrane porin
BURPS1106A_2049744-11.755306hypothetical protein
BURPS1106A_2050745-12.004160transposase IS911
BURPS1106A_2051544-11.227891hypothetical protein
BURPS1106A_2052643-7.944890hypothetical protein
BURPS1106A_2053433-5.532016DNA-binding response regulator
BURPS1106A_2054531-4.384057hypothetical protein
BURPS1106A_2055429-4.189190hypothetical protein
BURPS1106A_2056428-4.347664adenylylsulfate kinase
BURPS1106A_2057428-4.238883hypothetical protein
BURPS1106A_2058920-3.653805hypothetical protein
BURPS1106A_20591019-3.671961HlyD family secretion protein
BURPS1106A_20601119-3.831556ABC transporter permease
BURPS1106A_20611219-3.922150sulfotransferase domain-containing protein
BURPS1106A_20621219-3.777946hypothetical protein
BURPS1106A_20631219-3.585749type I secretion target repeat-containing
BURPS1106A_2064529-5.421852outer membrane efflux protein
BURPS1106A_2065234-6.964230ompA family protein
BURPS1106A_2066235-7.121092transposase
BURPS1106A_2067233-6.523264ISAfe7, transposase OrfA
BURPS1106A_2068546-11.601693hypothetical protein
BURPS1106A_2069652-12.798625hypothetical protein
BURPS1106A_2070858-14.088809transcriptional regulator
BURPS1106A_2071646-11.041327hypothetical protein
BURPS1106A_2072023-5.700160hypothetical protein
BURPS1106A_2073021-5.473996lipase
BURPS1106A_2074-118-3.486639hypothetical protein
BURPS1106A_2075-118-3.370823hypothetical protein
BURPS1106A_2076019-2.972380cyclic diguanylate phosphodiesterase
BURPS1106A_2077019-3.015045sensor histidine kinase/response regulator
BURPS1106A_2078524-2.203757LuxR family DNA-binding response regulator
BURPS1106A_2079526-1.960162hypothetical protein
BURPS1106A_2080625-1.062484hypothetical protein
BURPS1106A_2081624-0.817392hypothetical protein
BURPS1106A_2082621-0.954077hypothetical protein
BURPS1106A_2083617-0.950357outer membrane protein
BURPS1106A_2084522-1.692739hypothetical protein
BURPS1106A_2085620-1.903323hypothetical protein
BURPS1106A_2086522-2.447610hypothetical protein
BURPS1106A_2087522-2.627515major fimbrial subunit protein
BURPS1106A_2088524-2.746063fimbrial usher protein
BURPS1106A_2089429-3.392760fimbrial assembly chaperone protein
BURPS1106A_2090435-4.984122fimbrial protein
BURPS1106A_2091539-3.549568hypothetical protein
BURPS1106A_2092429-2.707643hypothetical protein
BURPS1106A_2093529-2.201333hypothetical protein
BURPS1106A_2094528-1.706084PHB depolymerase family esterase
BURPS1106A_2095931-3.155951hypothetical protein
BURPS1106A_2096825-3.159072hypothetical protein
BURPS1106A_2097826-5.048796EutG protein
BURPS1106A_2098122-4.565285hypothetical protein
BURPS1106A_2099-116-2.410485hypothetical protein
BURPS1106A_2100-214-1.209970hypothetical protein
BURPS1106A_2102-19-0.164160hypothetical protein
BURPS1106A_2101192.097790hypothetical protein
BURPS1106A_2103183.596144tryptophan halogenase
BURPS1106A_2104384.592243monovalent cation:proton antiporter-2 (CPA2)
BURPS1106A_2105294.363834Rieske family iron-sulfur cluster-binding
BURPS1106A_2106183.993459hypothetical protein
BURPS1106A_2107084.956034hypothetical protein
BURPS1106A_2108074.627732dihydroxyacetone kinase
BURPS1106A_2109093.337233hypothetical protein
BURPS1106A_2110-481.561674methyl-accepting chemotaxis protein
BURPS1106A_2111-3101.040044ABC-2 type transporter, permease
BURPS1106A_2112-3111.164227ABC transporter ATP-binding protein
BURPS1106A_2113-3120.610641ApbE family protein
BURPS1106A_2114-213-0.038857hypothetical protein
BURPS1106A_21150160.118930nitrous-oxide reductase
BURPS1106A_2116-1182.122964copper ABC transporter periplasmic
BURPS1106A_2117-1183.089637copper ABC transporter ATP-binding protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2048ECOLNEIPORIN924e-23 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 92.2 bits (229), Expect = 4e-23
Identities = 92/377 (24%), Positives = 136/377 (36%), Gaps = 57/377 (15%)

Query: 1 MKKLLIALPLAAAATTHAQSSVTLYGVLEDGVDYVSNVQGKHL----VQLASGV-TAGSR 55
MKK LIAL LAA A + VTLYG ++ GV+ +V V+ +G+ GS+
Sbjct: 1 MKKSLIALTLAALPVA-AMADVTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLGSK 59

Query: 56 WGVRGTEDLGGGLSAIFRLESGFDINSGRLGSGLAFSRNAYVGVGDAKLGTLTLGRQWDS 115
G +G EDLG GL AI+++E I G G +R +++G+ G L +GR
Sbjct: 60 IGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWG---NRQSFIGLK-GGFGKLRVGRLNSV 115

Query: 116 IVDY--VEPFTLNGNI-GGYYFAHPNDMDNTDNGFPISNAVKYRSPTIAGFTFGGLYAFG 172
+ D + P+ + G A P IS V+Y SP AG + YA
Sbjct: 116 LKDTGDINPWDSKSDYLGVNKIAEPEA-------RLIS--VRYDSPEFAGLSGSVQYALN 166

Query: 173 GQPGRFSDNATFSVGANYAAGPVGFGIGYLRINNPGVSTQGYQNYPGFTNAVYGNYLDAA 232
GR ++ ++ G NY G G Y+ + V
Sbjct: 167 DNAGR-HNSESYHAGFNYKNGGFFVQYGGA-----------YKRHHQVQENVNIEKYQIH 214

Query: 233 RAQKVFGVGASYQVV---QWLKLLADFTNTNFQQGSAGHDATFQNYELSALVKPTPAVTI 289
R + A Y V Q L + ++ Q AT + + + A
Sbjct: 215 RLVSGYDNDALYASVAVQQQDAKLVEENYSHNSQTEVA--ATLAYRFGNVTPRVSYAHGF 272

Query: 290 GAGYTYTTGRDHATNAEPKYHQFNLSVEYALSKRTSVYAMGAFQKAAGDAPVAQIAGFNP 349
+ T + Y Q + EY SKRTS + +
Sbjct: 273 KGSFDATNYNND-------YDQVVVGAEYDFSKRTSALVSAGWLQEG-----------KG 314

Query: 350 SGNQKQAVGRAGIRHVF 366
G G+RH F
Sbjct: 315 ESKFVSTAGGVGLRHKF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2053HTHFIS741e-17 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 74.5 bits (183), Expect = 1e-17
Identities = 38/163 (23%), Positives = 63/163 (38%), Gaps = 13/163 (7%)

Query: 3 IYLIEDDEIQAQYYQSMLVEHGWQVKILLDGERAFREIQRMPPDLIILDRRLPDLDGLEV 62
I + +DD L G+ V+I + +R I DL++ D +PD + ++
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDL 65

Query: 63 LMWVRKNYSNIPVLILTNAILESEVVAALEAGADDYVIKPPRKQEFVARVKALYRRATET 122
L ++K ++PVL+++ + A E GA DY+ KP E + + RA
Sbjct: 66 LPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELI----GIIGRALAE 121

Query: 123 RTLSELIEIGPYRIQTSEKVVYFHREAITLSPKEYEIIELLAR 165
R E + S EI +LAR
Sbjct: 122 PKR---------RPSKLEDDSQDGMPLVGRSAAMQEIYRVLAR 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2058SYCDCHAPRONE330.005 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 33.0 bits (75), Expect = 0.005
Identities = 21/126 (16%), Positives = 45/126 (35%), Gaps = 3/126 (2%)

Query: 898 LAPDDADAVLLRAELALDTGDFDEALSQFERLREQRPDAPESYANLIPALAALERRDDAI 957
++ D + + A +G +++A F+ L + L A+ + D AI
Sbjct: 31 ISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAI 90

Query: 958 AALQRALELNSKHPGALNNGVQFYLRTQQYDKA---MELAQRYVGAHGELASAHTMCGLV 1014
+ ++ K P + + L+ + +A + LAQ + E T +
Sbjct: 91 HSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRVSSM 150

Query: 1015 YHNLKA 1020
+K
Sbjct: 151 LEAIKL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2059RTXTOXIND2743e-89 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 274 bits (702), Expect = 3e-89
Identities = 94/439 (21%), Positives = 204/439 (46%), Gaps = 14/439 (3%)

Query: 41 SALGLEEASIAPARRAAALIPTVMLALLIVLVLWATFFKIDIIAAGQGKVIPSTTVQQLS 100
+ L L E ++ R A ++ L++ + + +++I+A GK+ S +++
Sbjct: 44 AHLELIETPVSRRPRLVAYF---IMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIK 100

Query: 101 TLEGGIVRELLVREGQIVKKGQPLVRLDPVVAQGAVTEQAATREGLMASIARLQAEADGK 160
+E IV+E++V+EG+ V+KG L++L + A+ + ++ R Q +
Sbjct: 101 PIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSI 160

Query: 161 ----------ATPLYPAGLKPEIVSEEEHVRAQRAEALNSTIEVLQQQRAAKQAEAADYR 210
Y + E V + ++ + + K+AE
Sbjct: 161 ELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVL 220

Query: 211 GRIPQYVNNQHLLDDQIQRMLPLVGVGSVAPNEITNLQRERGNLAAQIITTREGAAQASA 270
RI +Y N + ++ L+ ++A + + + + ++ + Q +
Sbjct: 221 ARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIES 280

Query: 271 QIAEASHKIEEKISTFRSEAREELARKQVQLQALEGTLSGKQDILDRTLIRSPVNGIVKT 330
+I A + + F++E ++L + + L L+ ++ ++IR+PV+ V+
Sbjct: 281 EILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQ 340

Query: 331 LYITTIGGVASPGKSVIDIVPTNDSLLIEARIQPQDIAYIRVGDDAKVRITAFDSGALGS 390
L + T GGV + ++++ IVP +D+L + A +Q +DI +I VG +A +++ AF G
Sbjct: 341 LKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGY 400

Query: 391 LDAKVELISPDSQADERSGSLYYKVQVRTHSSVVATQVGDLNILPGMVAEVDVITGRRTI 450
L KV+ I+ D+ D+R G L + V + + ++T ++ + GM ++ TG R++
Sbjct: 401 LVGKVKNINLDAIEDQRLG-LVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMRSV 459

Query: 451 MSYILRPIVRGMSRAMSER 469
+SY+L P+ ++ ++ ER
Sbjct: 460 ISYLLSPLEESVTESLRER 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2063RTXTOXINA471e-06 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 47.3 bits (112), Expect = 1e-06
Identities = 24/78 (30%), Positives = 38/78 (48%)

Query: 2958 AGADTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDMLVVNGDNIAHFSTPGSYWD 3017
G DT++G +G D L GG GND ++G G + L GG G+D V G+++A G +
Sbjct: 762 KGNDTLSGGNGDDQLYGGDGNDKLIGVAGNNYLNGGDGDDEFQVQGNSLAKNVLFGGKGN 821

Query: 3018 GGIMMGGEINTLQFDANN 3035
+ + L +
Sbjct: 822 DKLYGSEGADLLDGGEGD 839



Score = 45.7 bits (108), Expect = 3e-06
Identities = 28/77 (36%), Positives = 36/77 (46%), Gaps = 8/77 (10%)

Query: 2959 GADTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDML--------VVNGDNIAHFS 3010
G D + G+ G D L G GNDT+ G G D L GG GND L + GD F
Sbjct: 745 GDDLIEGNDGNDRLYGDKGNDTLSGGNGDDQLYGGDGNDKLIGVAGNNYLNGGDGDDEFQ 804

Query: 3011 TPGSYWDGGIMMGGEIN 3027
G+ ++ GG+ N
Sbjct: 805 VQGNSLAKNVLFGGKGN 821



Score = 39.6 bits (92), Expect = 3e-04
Identities = 22/60 (36%), Positives = 30/60 (50%), Gaps = 1/60 (1%)

Query: 2961 DTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDMLVVNGDNIAHFSTPG-SYWDGG 3019
D G+ G D++ G GND + G+ G D L GG G+D L N G +Y +GG
Sbjct: 738 DIFHGADGDDLIEGNDGNDRLYGDKGNDTLSGGNGDDQLYGGDGNDKLIGVAGNNYLNGG 797



Score = 38.4 bits (89), Expect = 5e-04
Identities = 20/43 (46%), Positives = 23/43 (53%)

Query: 2957 TAGADTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDML 2999
T AD GS D+ +G G+D I GN G D L G GND L
Sbjct: 725 TTRADKFFGSKFTDIFHGADGDDLIEGNDGNDRLYGDKGNDTL 767


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2065OMPADOMAIN1139e-32 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 113 bits (284), Expect = 9e-32
Identities = 59/180 (32%), Positives = 89/180 (49%), Gaps = 12/180 (6%)

Query: 78 QYQVRF--LGGLAYRGYWADSACRDIAARYADAAGLGVIAVAPCNPSDVAAPLPERVELP 135
Q+ + R ++ R+ V+A AP +V L
Sbjct: 163 QWTNNIGDAHTIGTRP-DNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK---HFTLK 218

Query: 136 TDTLFAFDKGGFEDISADGRRQLGDLVASIKAKILSINHLIVTGYTDRLGSDEHNARLSS 195
+D LF F+K + +G+ L L + + ++V GYTDR+GSD +N LS
Sbjct: 219 SDVLFNFNK---ATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSE 275

Query: 196 ERARTVADYMIAEGIPAAKITAVGRGAADPVV--VCNNGEQ-PELIRCLQKNRRVEIRIK 252
RA++V DY+I++GIPA KI+A G G ++PV C+N +Q LI CL +RRVEI +K
Sbjct: 276 RRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVK 335


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2072BACYPHPHTASE260.005 Salmonella/Yersinia modular tyrosine phosphatase si...
		>BACYPHPHTASE#Salmonella/Yersinia modular tyrosine phosphatase

signature.
Length = 468

Score = 25.9 bits (56), Expect = 0.005
Identities = 8/11 (72%), Positives = 9/11 (81%)

Query: 37 PKTPPFPPRRR 47
P+TPP PPR R
Sbjct: 156 PRTPPLPPRER 166


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2077HTHFIS817e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 81.0 bits (200), Expect = 7e-18
Identities = 36/146 (24%), Positives = 60/146 (41%), Gaps = 1/146 (0%)

Query: 854 TVLIAEDNLLNRSLLLDQLTTLGVRVIEAKNGEEALALLLKEPVDVVMTDIDMPMMDGFQ 913
T+L+A+D+ R++L L+ G V N + D+V+TD+ MP + F
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 914 LLAEMRRLGMTMPVYAVSASARPEDVAEGRARGFTDYLAKPVSLERLETVVRACCSAP-A 972
LL +++ +PV +SA + +G DYL KP L L ++ + P
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 973 GARADEDAQDELPGLPDVPPAYASAF 998
ED + L A +
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIY 150


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2078HTHFIS442e-07 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 44.4 bits (105), Expect = 2e-07
Identities = 19/84 (22%), Positives = 38/84 (45%), Gaps = 6/84 (7%)

Query: 10 KVVVADDHPIVLRAVTDYVNSLPGFHVVASVSSGDALLSAMREQEVNLVVTDFTMHQAND 69
++VADD + + ++ G+ V S+ L + + +LVVTD M
Sbjct: 5 TILVADDDAAIRTVLNQALSRA-GYDVRI-TSNAATLWRWIAAGDGDLVVTDVVM----P 58

Query: 70 DKDGLRLISHLMRAYERTPIIVFT 93
D++ L+ + +A P++V +
Sbjct: 59 DENAFDLLPRIKKARPDLPVLVMS 82


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2083OMADHESIN481e-07 Yersinia outer membrane adhesin signature.
		>OMADHESIN#Yersinia outer membrane adhesin signature.

Length = 455

Score = 48.4 bits (114), Expect = 1e-07
Identities = 52/159 (32%), Positives = 78/159 (49%)

Query: 935 ATGNNASASGTSSTAGGANAIASGENSTTNGANSTASGNGSSAFGESAAAAGDGSTALGA 994
A G NASA G S A GA A A+ + GA S A+G S A G + A GD + GA
Sbjct: 60 AGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGA 119

Query: 995 NAVASGVGSVATGAGSVASGANSSAYGTGSNATGAGSVAIGQGATASGSNSVALGTGSVA 1054
+ A G S + + + + ++A + ++ A+ S+A+G S
Sbjct: 120 ASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKT 179

Query: 1055 SEDNTVSVGSAGSERRITNVAAGVNATDAVNVGQLNSAV 1093
+N+VS+G R++T++AAG TDAVNV QL +
Sbjct: 180 DRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEI 218



Score = 41.0 bits (95), Expect = 2e-05
Identities = 74/321 (23%), Positives = 124/321 (38%), Gaps = 2/321 (0%)

Query: 634 ASGSNSTANGTNSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGD 693
A + N T S + A G A G +++A G +S A G + A+
Sbjct: 25 ADDYDGIPNLTAVQISPNADPALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKG 84

Query: 694 NSTASGTNASATGENSTATGTDSTASGSNSTANGANSTASGDNSTASGTNASATGENSTA 753
+ A G + ATG NS A G S A G ++ GA STA D +++ +
Sbjct: 85 AAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDGVAIGARASTSDTGVAVG 144

Query: 754 TGTASTASGSNSTANGTNSTASGNNSTASGTNASATGENSTATGTDSAASGTNSTANGTN 813
+ + A S + + ++ A+ S A G + ENS + G +S A GT
Sbjct: 145 FNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTK 204

Query: 814 STASGDNSTASGTNASATGENSTATGTASTASGSNSTANGANSTASGAGATATGENAAAT 873
T + + A + +T +A + +N+ A+ +S+ G T +A T
Sbjct: 205 DTDAVN--VAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAET 262

Query: 874 GAGATATGNNASASGTSSTAGGANAIASGENSTANGANSTASGAGATATGENAAATGAGA 933
A S + +N++A TA ++ + E+A A A
Sbjct: 263 LENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSVARTTLETAEEHANKKSAEA 322

Query: 934 TATGNNASASGTSSTAGGANA 954
A+ N + S +S T AN+
Sbjct: 323 LASANVYADSKSSHTLKTANS 343



Score = 39.9 bits (92), Expect = 6e-05
Identities = 81/333 (24%), Positives = 127/333 (38%), Gaps = 9/333 (2%)

Query: 422 ATASGTNSTANGTNSTASGDNSTASGTNASASGENSTATGTDSTASGSNSTANGTNSTAS 481
A A + N T S + A G A G +++A G +S A G + A+
Sbjct: 23 AFADDYDGIPNLTAVQISPNADPALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAA 82

Query: 482 GDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGDNSTASGTNASASGENS 541
+ A G + ATG NS A G S A G ++ G STA D A G AS S +
Sbjct: 83 KGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKD-GVAIGARASTS-DTG 140

Query: 542 TATGTDSTASGSNSTANGTNSTASGDN--STASGTNASATGENSTATGTDSTASGSNSTA 599
A G +S A NS A G +S + ++ S A G + ENS + G +S A
Sbjct: 141 VAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLA 200

Query: 600 NGTNST-----ASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGDNST 654
GT T A + ++ A +S+ G + + S
Sbjct: 201 AGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSA 260

Query: 655 ASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGDNSTASGTNASATGENSTATGT 714
+ NA + + + SNS A T TA ++ + T E++
Sbjct: 261 ETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSVARTTLETAEEHANKKSA 320

Query: 715 DSTASGSNSTANGANSTASGDNSTASGTNASAT 747
++ AS + + ++ T NS T +++T
Sbjct: 321 EALASANVYADSKSSHTLKTANSYTDVTVSNST 353



Score = 38.0 bits (87), Expect = 2e-04
Identities = 77/323 (23%), Positives = 121/323 (37%), Gaps = 6/323 (1%)

Query: 592 ASGSNSTANGTNSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGD 651
A + N T S + A G A G +++A G +S A G + A+
Sbjct: 25 ADDYDGIPNLTAVQISPNADPALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKG 84

Query: 652 NSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGDNSTASGTNASATGENSTA 711
+ A G + ATG NS A G S A G ++ G STA D ++T + A
Sbjct: 85 AAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDGVAIGAR--ASTSDTGVA 142

Query: 712 TGTDSTASGSNSTANGANSTASGDN--STASGTNASATGENSTATGTASTASGSNSTANG 769
G +S A NS A G +S + ++ S A G + ENS + G S A G
Sbjct: 143 VGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAG 202

Query: 770 TNSTASGNNSTASGTNASATGENSTATGTDSAASGTNSTANGTNSTASGDNSTASGTNAS 829
T T + N A + +T + + N+ A+ +S+ G + + + ++
Sbjct: 203 TKDTDAVN--VAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSA 260

Query: 830 ATGENSTATGTASTASGSNSTANGANSTASGAGATATGENAAATGAGATATGNNASASGT 889
T EN+ A + N +NS A TA + +A+
Sbjct: 261 ETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSVARTTLETAEEHANKKSA 320

Query: 890 SSTAGGANAIASGENSTANGANS 912
+ A S + T ANS
Sbjct: 321 EALASANVYADSKSSHTLKTANS 343



Score = 37.6 bits (86), Expect = 3e-04
Identities = 71/296 (23%), Positives = 116/296 (39%), Gaps = 9/296 (3%)

Query: 417 ASGDNATASGTNSTANGTNSTASGDNSTASGTNASASGENSTATGTDSTASGSNSTANGT 476
A G NA+A G +S A G + A+ + A G + A+G NS A G S A G ++ G
Sbjct: 60 AGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGA 119

Query: 477 NSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGDN--STASGTNA 534
STA D ++T + A G +S A NS A G +S + ++ S A G +
Sbjct: 120 ASTAQKDGVAIGAR--ASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRS 177

Query: 535 SASGENSTATGTDSTASGSNSTANGTNST-----ASGDNSTASGTNASATGENSTATGTD 589
ENS + G +S A GT T A + +
Sbjct: 178 KTDRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANAN 237

Query: 590 STASGSNSTANGTNSTASGDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTAS 649
+ A +S+ G + + S + NA + + + SNS A T TA
Sbjct: 238 AYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAE 297

Query: 650 GDNSTASGTNASATGENSTATGTDSTASGSNSTANGTNSTASGDNSTASGTNASAT 705
++ + T E++ ++ AS + + ++ T NS T +++T
Sbjct: 298 EHANSVARTTLETAEEHANKKSAEALASANVYADSKSSHTLKTANSYTDVTVSNST 353



Score = 36.0 bits (82), Expect = 8e-04
Identities = 49/147 (33%), Positives = 67/147 (45%)

Query: 844 ASGSNSTANGANSTASGAGATATGENAAATGAGATATGNNASASGTSSTAGGANAIASGE 903
A G N++A G +S A GA A A A A GAG+ ATG N+ A G S A G +A+ G
Sbjct: 60 AGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGA 119

Query: 904 NSTANGANSTASGAGATATGENAAATGAGATATGNNASASGTSSTAGGANAIASGENSTT 963
STA +T+ A + A A + A + A +IA G+ S T
Sbjct: 120 ASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKT 179

Query: 964 NGANSTASGNGSSAFGESAAAAGDGST 990
+ NS + G+ S + AAG T
Sbjct: 180 DRENSVSIGHESLNRQLTHLAAGTKDT 206



Score = 34.1 bits (77), Expect = 0.004
Identities = 40/119 (33%), Positives = 53/119 (44%)

Query: 939 NASASGTSSTAGGANAIASGENSTTNGANSTASGNGSSAFGESAAAAGDGSTALGANAVA 998
+ SA+ S+ A A + N S N A G A G NA A
Sbjct: 8 SVSAALISALFSSPYAFADDYDGIPNLTAVQISPNADPALGLEYPVRPPVPGAGGLNASA 67

Query: 999 SGVGSVATGAGSVASGANSSAYGTGSNATGAGSVAIGQGATASGSNSVALGTGSVASED 1057
G+ S+A GA + A+ + A G GS ATG SVAIG + A G ++V G S A +D
Sbjct: 68 KGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKD 126



Score = 33.3 bits (75), Expect = 0.006
Identities = 97/444 (21%), Positives = 158/444 (35%), Gaps = 23/444 (5%)

Query: 740 SGTNASATGENSTATGTASTASGSNSTANGTNSTASGNNSTASGTNASATGENSTATGTD 799
S A A + TA S + A G A G NASA G +S A G
Sbjct: 19 SSPYAFADDYDGIPNLTAVQISPNADPALGLEYPVRPPVPGAGGLNASAKGIHSIAIGAT 78

Query: 800 SAASGTNSTANGTNSTASGDNSTASGTNASATGENSTATGTASTASGSNSTANGANSTAS 859
+ A+ + A G S A+G NS A G + A G+++ G ASTA ST+
Sbjct: 79 AEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDGVAIGARASTSD 138

Query: 860 GAGATATGENAAATGAGATATGNNASASGTSSTAGGANAIASGENSTANGANSTASGAGA 919
A A A + A ++ +A+ S A G + ENS + G S
Sbjct: 139 TGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTH 198

Query: 920 TATGE------NAAATGAGATATGNNASASGTSSTAGGANAIASGENSTTNGANSTASGN 973
A G N A T N + A + +S AN+
Sbjct: 199 LAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSK 258

Query: 974 GSSAFGESAAAAGDGSTALGANAVASGVGSVATGAGSVASGANSSAYGTGSNATGAGSVA 1033
+ + A S + A A T + ANS A T A
Sbjct: 259 SAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSVARTTLETAE------ 312

Query: 1034 IGQGATASGSNSVALGTGSVASEDNTVSVGSAGSERRITNVAAGVNATDAVNVGQLNSAV 1093
A+ ++ AL + +V ++ + + V+ + +
Sbjct: 313 ----EHANKKSAEALASANVYADSKSSHTLKTANSYTDVTVSNSTKKAIRESNQYTDHKF 368

Query: 1094 SGIRNQMDGMQGQIDTLARDAYSGIAAATALTMIPDVDPGKTLAVGIGTANFKGYQASAL 1153
+ N++D + ++D G+A++ AL + + G ++ QA A+
Sbjct: 369 RQLDNRLDKLDTRVD-------KGLASSAALNSLFQPYGVGKVNFTAGVGGYRSSQALAI 421

Query: 1154 GATARITQNLKVKTGVSYSGSNYV 1177
G+ R+ +N+ +K GV+Y+GS+ V
Sbjct: 422 GSGYRVNENVALKAGVAYAGSSDV 445


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2088PF005777800.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 780 bits (2017), Expect = 0.0
Identities = 289/861 (33%), Positives = 445/861 (51%), Gaps = 43/861 (4%)

Query: 2 LAAALTALSATARGQQALEFDPAFLELGGGQGGADLSVYATSNRVLPGVYPVSVFVNGEA 61
L A + L F+P FL Q ADLS + + PG Y V +++N
Sbjct: 30 LFVACAFAAQAPLSSAELYFNPRFLA-DDPQAVADLSRFENGQELPPGTYRVDIYLNNGY 88

Query: 62 IERRDITFVSESARDGREDAIPCLSARMFDEWGVDIAAFAKLAQAGEDACVDIADSVPHA 121
+ RD+TF D + +PCL+ G++ A+ + + +DACV + + A
Sbjct: 89 MATRDVTFN---TGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDA 145

Query: 122 RTEFDSHQLRLNVTVPQAALKRRARGAVDPARWDQGIDAALLDYQLSAAQYAGGNFASAR 181
+ D Q RLN+T+PQA + RARG + P WD GI+A LL+Y S
Sbjct: 146 TAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNR---IGG 202

Query: 182 SRTTLYAGLRGAVNLGAWRLSHTSSFLRGL-----DGRNRFQIVNTFVQRDIAGWNSRLT 236
+ Y L+ +N+GAWRL +++ +N++Q +NT+++RDI SRLT
Sbjct: 203 NSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLT 262

Query: 237 AGEGTTPANIFDGFQFLGVQLNTDETMLPDSLQGYAPTVHGVAQTNAQVTIRQNGFVIYS 296
G+G T +IFDG F G QL +D+ MLPDS +G+AP +HG+A+ AQVTI+QNG+ IY+
Sbjct: 263 LGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYN 322

Query: 297 TYVPPGPFTIDDLYPTSSSGNLEVTITEADGHVTTFTQPYSAVPMLLRDGSWRYNVTAGQ 356
+ VPPGPFTI+D+Y +SG+L+VTI EADG FT PYS+VP+L R+G RY++TAG+
Sbjct: 323 STVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGE 382

Query: 357 YR-DGISGSHPSFAMATLARGLAGEFSLYGGFIGAGMYQSVLVGIGKNLGSIGAVSLDVS 415
YR P F +TL GL +++YGG A Y++ GIGKN+G++GA+S+D++
Sbjct: 383 YRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMT 442

Query: 416 HARSAVDLADSSTVSGHAFRVLYAKAVGSWGTDFRLLAYRYSTAGYRSFADAVQLRDGSE 475
A S L D S G + R LY K++ GT+ +L+ YRYST+GY +FAD R
Sbjct: 443 QANS--TLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGY 500

Query: 476 PAAL------------------GAKRQRLEGTVNQRLGRLGSMYATVAVQTYWGSAARST 517
KR +L+ TV Q+LGR ++Y + + QTYWG++
Sbjct: 501 NIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDE 560

Query: 518 VYQLGHSGNWGRASYGLYAAYSKGSGVPSSWN-VSLSLSMPLEVLFGGARVRAPAGGSAN 576
+Q G + + ++ L + +K + ++L++++P A+
Sbjct: 561 QFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQW--RHAS 618

Query: 577 VSYFVSRNNENHVNQQMTASGSSSEQ-RLNYSVGVAHS----SESDVSGSVSASYLAPFG 631
SY +S + + G+ E L+YSV ++ S +G + +Y +G
Sbjct: 619 ASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYG 678

Query: 632 RYDASIGSGRGYTQAAFTAAGGMLWHGTGVLFTQPLGETVAVVDVPNVQGVRFEMHPGVS 691
+ Q + +GG+L H GV QPL +TV +V P + + E GV
Sbjct: 679 NANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVR 738

Query: 692 TDRAGEAVIPRLNPYRVNRIVVDQRRMPQDVEIRNPVSEVVPTRAAVVQTHFDSVVGLRA 751
TD G AV+P YR NR+ +D + +V++ N V+ VVPTR A+V+ F + VG++
Sbjct: 739 TDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL 798

Query: 752 LFTLMRADGSFPPQGATAENDEGQVLGVVGMDGETFVAGLPAAEGHFVVRWGAARQNRCR 811
L TL + P GA ++ Q G+V +G+ +++G+P A G V+WG C
Sbjct: 799 LMTL-THNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLA-GKVQVKWGEEENAHCV 856

Query: 812 VNYALPGKAAIGAYLAVEAIC 832
NY LP ++ + A C
Sbjct: 857 ANYQLPPESQQQLLTQLSAEC 877


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2105PF07675320.003 Cleaved Adhesin
		>PF07675#Cleaved Adhesin

Length = 1358

Score = 32.4 bits (73), Expect = 0.003
Identities = 25/72 (34%), Positives = 32/72 (44%), Gaps = 12/72 (16%)

Query: 181 DYVIADPEPRGGRLAME-RGVTWAARRHDHRF--GAHYPWTLRLTPPQDGAPASVEIDT- 236
DY I +PEP G++ + G AR D F G Y +T+R DG VE D+
Sbjct: 472 DYCITNPEPASGKMWIAGDGGNQPARYDDFAFEAGKKYTFTMRRAGMGDGTDMEVEDDSP 531

Query: 237 --------RDGT 240
RDGT
Sbjct: 532 ASYTYTVYRDGT 543


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2107INFPOTNTIATR260.035 Macrophage infectivity potentiator signature.
		>INFPOTNTIATR#Macrophage infectivity potentiator signature.

Length = 233

Score = 25.7 bits (56), Expect = 0.035
Identities = 12/26 (46%), Positives = 14/26 (53%)

Query: 47 AAAAAAAMAMAMAANDVARRRTDADR 72
AA AM+ AMAA D TD D+
Sbjct: 8 AAIMGLAMSTAMAATDATSLTTDKDK 33


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2111ABC2TRNSPORT562e-11 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 56.1 bits (135), Expect = 2e-11
Identities = 62/201 (30%), Positives = 100/201 (49%), Gaps = 10/201 (4%)

Query: 17 ASPLRILFGLTQPLLYLFVLGAALRSGTYAEIGG--YQAYIFPGVVGLSLM----FTAIS 70
A+ +L L +PL+YLF LGA L +GG Y A++ G+V S M F I
Sbjct: 30 AALASLLGHLAEPLIYLFGLGAGL-GVMVGRVGGVSYTAFLAAGMVATSAMTAATFETIY 88

Query: 71 AAVGIVHDRQTGLLNALLVSPVRRVDIALGKIGAGALLAWLQALLLLPFSPAIGIGLTAP 130
AA G + ++T A+L + +R DI LG++ A A L + + A+G
Sbjct: 89 AAFGRMEGQRT--WEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGY-TQWL 145

Query: 131 RLALLVAAMAFAALAFSALGLALALPFRSVIVFPVVSNTLLLPMFFLSGGLYPLDLAPDW 190
L + +A LAF++LG+ + S F ++ P+ FLSG ++P+D P
Sbjct: 146 SLLYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIV 205

Query: 191 IRAAAAFDPAAYGVDLMRGVL 211
+ AA F P ++ +DL+R ++
Sbjct: 206 FQTAARFLPLSHSIDLIRPIM 226


29BURPS1106A_2142BURPS1106A_2164Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_21422141.168086LtxA protein
BURPS1106A_21430101.496819amino acid adenylation:thioester reductase
BURPS1106A_2145-191.655156hypothetical protein
BURPS1106A_2146082.457608polyhydroxybutyrate depolymerase
BURPS1106A_2147083.089298hypothetical protein
BURPS1106A_2148092.277569hypothetical protein
BURPS1106A_21491112.533507hypothetical protein
BURPS1106A_21501123.533679LacI family transcriptional regulator
BURPS1106A_21520124.036856hypothetical protein
BURPS1106A_21510143.549880gluconate 2-dehydrogenase
BURPS1106A_21530133.659397major facilitator transporter
BURPS1106A_21540154.8336082-keto-gluconokinase
BURPS1106A_2155-1134.164467hypothetical protein
BURPS1106A_2156-2132.587211hypothetical protein
BURPS1106A_2157-1143.072159hypothetical protein
BURPS1106A_21580142.010950LysR family transcriptional regulator
BURPS1106A_2159-1122.504598hypothetical protein
BURPS1106A_2160-1122.093673voltage-gated ClC-type chloride channel ClcB
BURPS1106A_2161-1102.198357TetR family transcriptional regulator
BURPS1106A_2162-292.701052RND family efflux transporter MFP subunit
BURPS1106A_2163-282.528791AcrB/AcrD/AcrF family protein
BURPS1106A_21640113.893049NodT family efflux transporter outer membrane
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2150HTHTETR353e-04 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 34.6 bits (79), Expect = 3e-04
Identities = 15/107 (14%), Positives = 40/107 (37%), Gaps = 1/107 (0%)

Query: 12 ATISDVAREAGTGKTSVSRYLNGETNVLSADLRQRIETAIERLNYRPNQMARGL-KRGRN 70
++ ++A+ AG + ++ + ++++ S E + R
Sbjct: 32 TSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLRE 91

Query: 71 RLLGLLAADLTNPYTVEVLRGVEAACHALGYMPLICHAANELEMERR 117
L+ +L + +T ++ + C +G M ++ A L +E
Sbjct: 92 ILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESY 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2153TCRTETB310.009 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 31.0 bits (70), Expect = 0.009
Identities = 32/139 (23%), Positives = 53/139 (38%), Gaps = 2/139 (1%)

Query: 244 IGVYGFVLWLPSIVKNGSALGMVATGWLSALP-YLAATIAMLAASWASDRLGSRKGFVWP 302
V GFV +P ++K+ L G + P ++ I DR G
Sbjct: 270 GTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIG 329

Query: 303 FLLIGAAAFAASYTLGSTHFWLSYALLVVAGAAMYAPYGPFFAIVPELLPKNVAGGAMAL 362
+ + AS+ L +T ++++ ++ V G + IV L + AG M+L
Sbjct: 330 VTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKT-VISTIVSSSLKQQEAGAGMSL 388

Query: 363 INSMGALGSFVGSYAVGYL 381
+N L G VG L
Sbjct: 389 LNFTSFLSEGTGIAIVGGL 407



Score = 30.6 bits (69), Expect = 0.013
Identities = 24/140 (17%), Positives = 55/140 (39%), Gaps = 5/140 (3%)

Query: 35 AAAGINQDLGISKGLSSLIGALFFLGYFFFQIPGAIYAERRSVKTLVFWSLVLWGACASL 94
+ I D ++ + F L + +++ +K L+ + +++ S+
Sbjct: 36 SLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCF-GSV 94

Query: 95 TGVV--SNIPSLMAIRFLLGVVEAAVMPAML-IFISNWFTKRERSRANTFLILGNPVTVL 151
G V S L+ RF+ G AA PA++ + ++ + K R +A + +
Sbjct: 95 IGFVGHSFFSLLIMARFIQGA-GAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEG 153

Query: 152 WMSVVSGYLVHEFGWRHMFV 171
+ G + H W ++ +
Sbjct: 154 VGPAIGGMIAHYIHWSYLLL 173



Score = 30.6 bits (69), Expect = 0.014
Identities = 27/109 (24%), Positives = 46/109 (42%), Gaps = 6/109 (5%)

Query: 268 TGWLSALPYLAATIAMLAASWASDRLGSRKGFVWPFLLIGAAAFAASYTLGSTHF-WLSY 326
T W++ L +I SD+LG ++ ++ ++ + +G + F L
Sbjct: 51 TNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGF--VGHSFFSLLIM 108

Query: 327 ALLVVA-GAAMYAPYGPFFAIVPELLPKNVAGGAMALINSMGALGSFVG 374
A + GAA + +V +PK G A LI S+ A+G VG
Sbjct: 109 ARFIQGAGAAAFPAL--VMVVVARYIPKENRGKAFGLIGSIVAMGEGVG 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2161HTHTETR617e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 61.2 bits (148), Expect = 7e-14
Identities = 41/203 (20%), Positives = 76/203 (37%), Gaps = 10/203 (4%)

Query: 5 RLTREQSKDLTRERLLSAAHAIFTKKGYVAASVEDIASAAGYTRGAFYSNFRSKAELLIE 64
R T++++++ TR+ +L A +F+++G + S+ +IA AAG TRGA Y +F+ K++L E
Sbjct: 3 RKTKQEAQE-TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 65 LLKRDHEEAEADLQKIFE--SGGTREQMEA---HALEYYSQFFRNNPAFLLWGEAKLQAT 119
+ + + G + H LE R +
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121

Query: 120 RDAKFRARFNEFVKEKRDRFTHYILTFAERVGTPLLLPADVLALGLMSLCDGVQSYHAAD 179
A + E DR + E P L A+ + G+
Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFA 181

Query: 180 PRHVTGDAAQQVLAGFFARVVLA 202
P+ D ++ A + ++L
Sbjct: 182 PQSF--DLKKE--ARDYVAILLE 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2162RTXTOXIND371e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.7 bits (85), Expect = 1e-04
Identities = 30/200 (15%), Positives = 59/200 (29%), Gaps = 32/200 (16%)

Query: 1 MNRSGSRAALLIGVALIAAACHRKEAAPSAPRPVVAVPAQADGAAAAVSLPGEIQPRYAT 60
+ SR L+ ++ + +VA A+G EI+P
Sbjct: 49 IETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVAT---ANGKLTHSGRSKEIKP---- 101

Query: 61 PLSFRIAGKLVER-KVRLGDIVKKGQVVALLDTSDVARSAASAQAQLDAATHALTFAQQQ 119
I +V+ V+ G+ V+KG V+ L A+A +L A+ +
Sbjct: 102 -----IENSIVKEIIVKEGESVRKGDVLLKLTALG-------AEADTLKTQSSLLQARLE 149

Query: 120 RERDRA--QARENLIAPAQLEQTENAYASARAQRDQAAQQLA----------LAKNQLQY 167
+ R + ++ E P E + + + L + +L
Sbjct: 150 QTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNL 209

Query: 168 ATLVADHAGYITAEQADTGQ 187
A+ +
Sbjct: 210 DKKRAERLTVLARINRYENL 229



Score = 34.8 bits (80), Expect = 5e-04
Identities = 10/71 (14%), Positives = 27/71 (38%)

Query: 100 ASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYASARAQRDQAAQQLA 159
+ A+++ + + + + + + IA + + EN Y A + QL
Sbjct: 217 LTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLE 276

Query: 160 LAKNQLQYATL 170
++++ A
Sbjct: 277 QIESEILSAKE 287


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2163ACRIFLAVINRP433e-137 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 433 bits (1114), Expect = e-137
Identities = 223/1062 (20%), Positives = 423/1062 (39%), Gaps = 75/1062 (7%)

Query: 13 LSAWALRHQALVVYLIALATIAGILAYSRLAQSEDPPFTFRVMVIRTFWPGATARQVQEQ 72
++ + +R L + +AG LA +L ++ P + + +PGA A+ VQ+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 73 VTDRIGRKLQEMPAIDYLRSYS-RPGESMLFFAMKDSAPVKDVPQTWYQVRKKVGDISMT 131
VT I + + + + Y+ S S G + + QV+ K+ +
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQV---QVQNKLQLATPL 117

Query: 132 LPPGVQGP-FFNDEFGDVYTNIYTLEGDG--FSPAQLHDYAD-QLRVVLLRVPGVAKVDY 187
LP VQ ++ Y + D + + DY ++ L R+ GV V
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 188 FGDPDQRIFVEIDNTRLARLGISPQQIAQAINAQNDVASPGVLTAAHD------RVFIRP 241
FG + + +D L + ++P + + QND + G L I
Sbjct: 178 FG-AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 242 SGQYESVAAIADTLIRVN--GRTFRLGELATIKRGYDDPPVTQMRTIGRNANGRAVLGIG 299
++++ +RVN G RL ++A ++ G + + NG+ G+G
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELG------GENYNVIARINGKPAAGLG 290

Query: 300 VTMQPGGDVIRLGKALDASAKALQAQLPAGLALTEVSSMPHAVARSVDDFLEAVAEAVAI 359
+ + G + + KA+ A LQ P G+ + V S+ + ++ + EA+ +
Sbjct: 291 IKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIML 350

Query: 360 VLIVSLVSLG-LRTGMVVVISIPVVLAVTALFMYLFDIGLHKVSLGTLVLALGLLVDDAI 418
V +V + L +R ++ I++PVVL T + F ++ +++ +VLA+GLLVDDAI
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAI 410

Query: 419 IAVEMMA-VKLEQGFSRARAAAFAYTSTAFPMLTGTLVTVSGFLPIALAKSSTGEYTRSI 477
+ VE + V +E A + + ++ +V + F+P+A STG R
Sbjct: 411 VVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQF 470

Query: 478 FEVSAIALIASWFAAVVLIPLLGYHMLPERKHPRQDAAGAPHAP-DAAHDHAHGHDIYDT 536
A+ S A++L P L +L + G + DH+
Sbjct: 471 SITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHS-------V 523

Query: 537 RFYTRLRVWIKWCIERRFIVLAITIALFVVALAGFSLVPQQFFPSSDRPELLVDLRLPEG 596
YT + + L I + + F +P F P D+ L ++LP G
Sbjct: 524 NHYTNS---VGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAG 580

Query: 597 ASFNATLKEAERLEKLIAK--RPEIDHAVNFVGSGAPRFYLPLDQQLQLPNFAQFVITAK 654
A+ T K +++ K + ++ G Q N ++ K
Sbjct: 581 ATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSG---------QAQNAGMAFVSLK 631

Query: 655 SVDAR---EKLSAWLAPVLREQFPAARTRISRLENGPPV-------GYPVQ-FRVSGDSI 703
+ R E + + + + R N P + G+ + +G
Sbjct: 632 PWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGH 691

Query: 704 ATVRAIAEKVAATMR---ADARATNVQFDWDEPAERSVRFELDQHKARELNVSSQDVASF 760
+ ++ A + D + E+DQ KA+ L VS D+
Sbjct: 692 DALTQARNQLLGMAAQHPASLVSVRPNGLEDTA---QFKLEVDQEKAQALGVSLSDINQT 748

Query: 761 LAMTLSGTTLTQYRERDKLIAVDLRAPRAQRIDPASLAGLAMPTPNG-PVPLGSLGRFHD 819
++ L GT + + +R ++ + ++A R+ P + L + + NG VP + H
Sbjct: 749 ISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHW 808

Query: 820 TLEYGVVWERDRQPTITVQSDVTAGAQGIDVTHAIDAKLDALRAQLPVGYRIEIGGSVEE 879
+ + P++ +Q + G + A ++ L ++LP G + G +
Sbjct: 809 VYGSPRLERYNGLPSMEIQGEAAPG----TSSGDAMALMENLASKLPAGIGYDWTGMSYQ 864

Query: 880 STKGQTSINAQMPLMVIAVLTLLMIQLQSFSRVLMVVLTAPLGMIGVVGTLLLFGKPFGF 939
A + + + V L +S+S + V+L PLG++GV+ LF +
Sbjct: 865 ERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDV 924

Query: 940 VAMLGVIAMFGIIMRNSVILVDQIEQDIAA-GHGRFDAIVGATVRRFRPITLTAAAAVLA 998
M+G++ G+ +N++++V+ + + G G +A + A R RPI +T+ A +L
Sbjct: 925 YFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILG 984

Query: 999 LIPLLRSNFFG-----PMATALMGGITSATVLTLFFLPALYA 1035
++PL SN G + +MGG+ SAT+L +FF+P +
Sbjct: 985 VLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFV 1026



Score = 84.1 bits (208), Expect = 2e-18
Identities = 91/535 (17%), Positives = 182/535 (34%), Gaps = 67/535 (12%)

Query: 550 IERRFIVLAITIALFVVALAGFSLVPQQFFPSSDRPELLVDLRLPEGASFNATLKEAER- 608
I R + I L + +P +P+ P + V P A + +
Sbjct: 6 IRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYP-----GADAQTVQDT 60

Query: 609 ----LEKLIAKRPEIDH---AVNFVGSG--APRFYLPLDQQLQLPNFAQFVITAKSVDAR 659
+E+ + + + + GS F D P+ AQ V +
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTD-----PDIAQ-------VQVQ 108

Query: 660 EKLSAWLAPVLREQFP-AARTRISRLENGPPVGYPVQFRVSGDSIATVRAIAEKVAATMR 718
KL P + + +E V VS + T I++ VA+ ++
Sbjct: 109 NKLQL-----ATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVK 163

Query: 719 ADAR----ATNVQFDWDEPAERSVRFELDQHKARELNVSSQDVASFL--------AMTLS 766
+VQ A+ ++R LD + ++ DV + L A L
Sbjct: 164 DTLSRLNGVGDVQLF---GAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLG 220

Query: 767 GTTLTQYRERDKLIAVDLRAPRAQRIDPASLAGLAMPTPNG-PVPLGSLGRFHDTLE-YG 824
GT ++ + I R + +L +G V L + R E Y
Sbjct: 221 GTPALPGQQLNASIIAQTRFKNPEEFGKVTLRV----NSDGSVVRLKDVARVELGGENYN 276

Query: 825 VVWERDRQPTITVQSDVTAGAQGIDVTHAIDAKLDALRAQLPVGYRIEI----GGSVEES 880
V+ + +P + + GA +D AI AKL L+ P G ++ V+ S
Sbjct: 277 VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLS 336

Query: 881 TKGQTSINAQMPLMVIAVLTLLMIQLQSFSRVLMVVLTAPLGMIGVVGTLLLFGKPFGFV 940
+ +++ L + + LQ+ L+ + P+ ++G L FG +
Sbjct: 337 I--HEVVKTLFEAIMLVFLVMYLF-LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTL 393

Query: 941 AMLGVIAMFGIIMRNSVILVDQIEQDIAAGHGRF-DAIVGATVRRFRPITLTAAAAVLAL 999
M G++ G+++ +++++V+ +E+ + +A + + + A
Sbjct: 394 TMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVF 453

Query: 1000 IPLL-----RSNFFGPMATALMGGITSATVLTLFFLPALYAAWFRVKPDERDPEP 1049
IP+ + + ++ + + ++ L PAL A + E
Sbjct: 454 IPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK 508


30BURPS1106A_2256BURPS1106A_2261Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_225628-1.288137hypothetical protein
BURPS1106A_225728-1.0918714-carboxymuconolactone decarboxylase
BURPS1106A_225829-1.103136MerR family transcriptional regulator
BURPS1106A_225949-0.941241comE protein
BURPS1106A_226028-1.480843hypothetical protein
BURPS1106A_226128-1.208674clpB protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2259PF06776300.007 Invasion associated locus B
		>PF06776#Invasion associated locus B

Length = 214

Score = 29.5 bits (66), Expect = 0.007
Identities = 14/65 (21%), Positives = 22/65 (33%), Gaps = 1/65 (1%)

Query: 119 PTTSSATPAPSAATPAPATATPSTAASAPAA-KKSRSSKKQDKAAAASAAAQASAPAAAS 177
P T+ A PA A PA +P A+ A + A A + + A
Sbjct: 15 PVTNHAVPALKAIQMGPAELSPMLASCRRLARRNGARLMLAGAMAIALSFGWSDRADAQG 74

Query: 178 TTKAK 182
++
Sbjct: 75 AVRSV 79


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2261HTHFIS340.002 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 34.4 bits (79), Expect = 0.002
Identities = 34/160 (21%), Positives = 58/160 (36%), Gaps = 28/160 (17%)

Query: 576 VVGQNEAIDAVADAIRRSRAGLADPNRPYGSFLFLGPTGVGKTELCKALAGFLFDSEEHL 635
+VG++ A+ + + R L + + G +G GK + +AL +
Sbjct: 139 LVGRSAAMQEIYRVLAR----LMQTDLT---LMITGESGTGKELVARALHDYGKRRNGPF 191

Query: 636 IRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSV-------ILLDEIEKA 688
+ I+M+ + L G+E+G + T A R + LDEI
Sbjct: 192 VAINMAAIPRDLIESEL-------FGHEKGAF-TGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 689 HPDVFNVLLQVLDDG---RMTDGQGRTVDFKNTVIVMTSN 725
D LL+VL G + D + IV +N
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN 280



Score = 32.9 bits (75), Expect = 0.006
Identities = 34/166 (20%), Positives = 63/166 (37%), Gaps = 27/166 (16%)

Query: 136 LESAIAAVRGGSQ-------VHSQDAESQREALKKYTVDLTERARAG-KLDPVIGRDDEI 187
+AI A G+ ++ AL + ++ P++GR +
Sbjct: 87 FMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAM 146

Query: 188 RRSIQILQRRTKNN-PVLI-GEPGVGKTAIVEGLAQRIVNGEVPETLKNKRVLSLDMAAL 245
+ ++L R + + ++I GE G GK + L +N ++++MAA+
Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDY-------GKRRNGPFVAINMAAI 199

Query: 246 --------LAGAKYRGEFEERLKAVLNDIAKDEGRTIVFIDEIHTM 283
L G + +G F + EG T+ F+DEI M
Sbjct: 200 PRDLIESELFGHE-KGAFTGAQTRSTGRFEQAEGGTL-FLDEIGDM 243


31BURPS1106A_2284BURPS1106A_2292Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2284-113-4.157321phosphate transporter family protein
BURPS1106A_2285114-4.629634hypothetical protein
BURPS1106A_2286217-4.580142replicative DNA helicase
BURPS1106A_2287315-1.256785hypothetical protein
BURPS1106A_2288213-0.03188050S ribosomal protein L9
BURPS1106A_22892130.65735530S ribosomal protein S18
BURPS1106A_22901131.699638primosomal replication protein N
BURPS1106A_22911112.47832230S ribosomal protein S6
BURPS1106A_22923123.119104hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2288UREASE270.046 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 26.6 bits (59), Expect = 0.046
Identities = 11/33 (33%), Positives = 17/33 (51%), Gaps = 5/33 (15%)

Query: 121 LKMIGEHGVQVALHTDVV-----VDVTVNVIGD 148
L + E+ VQV +HTD + V+ T+ I
Sbjct: 235 LSVADEYDVQVMIHTDTLNESGFVEDTIAAIKG 267


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2292PF03544290.033 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 28.8 bits (64), Expect = 0.033
Identities = 15/131 (11%), Positives = 36/131 (27%), Gaps = 2/131 (1%)

Query: 85 LPASDVPVSDVPVSDVPVSDMPVSDVPVSDMPVSDMPVSDVPVSDVPVSDMPVSDVPVSD 144
+ A + S V ++P P+S V+ + P V + P+ +
Sbjct: 28 VVAGLLYTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEP--EPIPE 85

Query: 145 APEQDTPSQDMRAPNKPAVDRPPEAKPRFGADARGGARAGRWFARPGSRGPTLDRPGPGP 204
P++ + P +P + + D + +
Sbjct: 86 PPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAA 145

Query: 205 RAAALPGLGWG 215
+ + + G
Sbjct: 146 TSKPVTSVASG 156


32BURPS1106A_2340BURPS1106A_2387Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2340-1133.458888peptidyl-prolyl cis-trans isomerase family
BURPS1106A_2341-1134.213677acetyltransferase
BURPS1106A_2342-1133.192182phosphoribosylformylglycinamidine synthase
BURPS1106A_23430123.776471hypothetical protein
BURPS1106A_2344-2123.782068D-amino acid dehydrogenase small subunit
BURPS1106A_2346-2102.036367carbohydrate kinase family protein
BURPS1106A_2345-3100.190700hypothetical protein
BURPS1106A_2347-3110.048983glucose-6-phosphate isomerase
BURPS1106A_2348-3130.355688ABC transporter ATP-binding protein
BURPS1106A_2349-3140.134329acyl-CoA thioesterase
BURPS1106A_2350-318-0.508089PpiC-type peptidyl-prolyl cis-trans isomerase
BURPS1106A_23529310.283997*hypothetical protein
BURPS1106A_2353429-1.237969hypothetical protein
BURPS1106A_2354329-1.823204hypothetical protein
BURPS1106A_2355130-2.626912hypothetical protein
BURPS1106A_2356213-3.856738hypothetical protein
BURPS1106A_2357310-4.657430hypothetical protein
BURPS1106A_2358311-4.922086ribosomal subunit interface protein
BURPS1106A_2361410-4.696030*hypothetical protein
BURPS1106A_2362410-4.401677hypothetical protein
BURPS1106A_236339-2.405423ATP-dependent protease La
BURPS1106A_236418-1.431056ATP-dependent protease ATP-binding subunit ClpX
BURPS1106A_2365190.850855ATP-dependent Clp protease proteolytic subunit
BURPS1106A_2366382.720831trigger factor
BURPS1106A_23671104.292940hypothetical protein
BURPS1106A_2368-283.783850glycerate kinase
BURPS1106A_2369-293.122826MarR family transcriptional regulator
BURPS1106A_2370-272.879107hypothetical protein
BURPS1106A_2371-272.1939732-dehydropantoate 2-reductase
BURPS1106A_2372-271.298681LuxR family transcriptional regulator
BURPS1106A_2373-111-0.036412outer membrane porin
BURPS1106A_2374-215-1.053013major facilitator family transporter
BURPS1106A_2375-125-4.474336histone deacetylase family protein
BURPS1106A_2376232-5.255057endonuclease Nuc
BURPS1106A_2377031-4.605401hypothetical protein
BURPS1106A_2378032-4.204578exported avidin family protein
BURPS1106A_2379030-3.981862hypothetical protein
BURPS1106A_2380132-4.602325DNA adenine methylase
BURPS1106A_2381030-4.163576PAAR motif-containing protein
BURPS1106A_2382-126-3.445905hypothetical protein
BURPS1106A_2383122-2.018172hypothetical protein
BURPS1106A_2384015-0.994513hypothetical protein
BURPS1106A_2385012-0.011178hypothetical protein
BURPS1106A_23872121.412795hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2346PYOCINKILLER300.024 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 30.1 bits (67), Expect = 0.024
Identities = 53/222 (23%), Positives = 73/222 (32%), Gaps = 21/222 (9%)

Query: 84 DALVAAAELRRLGFAADAWMPIEVKPDDARWALERARAANVPIDEAAPESFDGYGWLVDG 143
+ L AA AA A E +A+ E I A + G +V
Sbjct: 205 NTLTAAKASIE---AAAANKAREQAAAEAKRKAEEQARQQAAIRAANTYAMPANGSVVAT 261

Query: 144 LFGIGLARPLDGAFAAIAQRIAARARHTGRVLALDVPSGLDSDTGARVGGGTAVTATCTL 203
G GL + GA A++AQ I+ GRVLA S G ++T +
Sbjct: 262 AAGRGLIQVAQGA-ASLAQAISDAIAVLGRVLA--------SAPSVMAVGFASLTYSSRT 312

Query: 204 SFIAAKPGLYTGDGRDLAGEIHVAPLDLGEPPAPAIRLNAPELFEAR--LPERAFASHKG 261
+ T D A + A L P++ LNA LP R +G
Sbjct: 313 AEQWQD---QTPDSVRYALGMDAAKLG----LPPSVNLNAVAKASGTVDLPMRLTNEARG 365

Query: 262 TYGSLGIVGGDTGMCGAPILAARAALFAGAGKVHVGFVGTGA 303
+L +V D + AA A G V T A
Sbjct: 366 NTTTLSVVSTDGVSVPKAVPVRMAAYNATTGLYEVTVPSTTA 407


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2363GPOSANCHOR403e-05 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 40.4 bits (94), Expect = 3e-05
Identities = 35/192 (18%), Positives = 73/192 (38%), Gaps = 15/192 (7%)

Query: 92 KVLVEGLQRAQALSIEEQETQFSCEVMPLEPDHADSAETEALRRAIVSQFDQYVKLNKKI 151
L + L+ A S + + E A A+ E ++ K +
Sbjct: 193 AELEKALEGAMNFSTADSAKIKTLEAE-KAALAARKADLEKALEGAMNFSTADSAKIKTL 251

Query: 152 PPEILTSLSGIDEAGRLADTIAAHLPLKLDQKQHILEMFPVIERLEHLLAQLEAEIDILQ 211
E + E + + + + + LE A LE + +L
Sbjct: 252 EAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAE---KAALEAEKADLEHQSQVLN 308

Query: 212 VEKRIRGRVKRQMEKSQREYYLNEQVKAIQKELGEGEEGAD--LEELEKRINAARMPKEA 269
R ++R ++ S+ +Q++A ++L E + ++ + L + ++A+R EA
Sbjct: 309 AN---RQSLRRDLDASREAK---KQLEAEHQKLEEQNKISEASRQSLRRDLDASR---EA 359

Query: 270 KKKADAELKKLK 281
KK+ +AE +KL+
Sbjct: 360 KKQLEAEHQKLE 371


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2364HTHFIS310.009 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.0 bits (70), Expect = 0.009
Identities = 19/112 (16%), Positives = 40/112 (35%), Gaps = 12/112 (10%)

Query: 51 EAAAAGVEASLSKSDLPSPQEIRDILDQYVIGQERAKKILAVAVYNHYKRL-------KH 103
+A+ G L K E+ I+ + + +R L + + +
Sbjct: 92 KASEKGAYDYLPKP--FDLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEI 149

Query: 104 LDKKDDVELSKSNILLIGPTGSGKTLLAQTLARL---LNVPFVIADATTLTE 152
+ + +++ G +G+GK L+A+ L N PFV + +
Sbjct: 150 YRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPR 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2373ECOLNEIPORIN671e-14 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 67.1 bits (164), Expect = 1e-14
Identities = 72/323 (22%), Positives = 118/323 (36%), Gaps = 37/323 (11%)

Query: 20 AATLAALSGPAHAQSTLTLYGVADAGVQYLSRADGRHAAWRLQN-----YGILPSQLGIK 74
A TLAAL P A + +TLYG AGV SR+ + A L S++G K
Sbjct: 7 ALTLAAL--PVAAMADVTLYGTIKAGV-ETSRSVAHNGAQAASVETGTGIVDLGSKIGFK 63

Query: 75 GEEDLGGGWRARFQLEQGINLNDGTATVPGYAFFRGAYVGMGGPAGTVTLGRQFSTLFDK 134
G+EDLG G +A +Q+EQ ++ + R +++G+ G G + +GR S L D
Sbjct: 64 GQEDLGNGLKAIWQVEQKASIAGTDSGW----GNRQSFIGLKGGFGKLRVGRLNSVLKDT 119

Query: 135 TLFYDPLWYASYSGQGVLVPLSANFVDHSIKFQSATFAGFDVEALAAMAGIAGNTRAGRV 194
+P S + + S+++ S FAG ++ A N AGR
Sbjct: 120 GDI-NPWDSKSDYLGVNKIAEPEARLI-SVRYDSPEFAGL-SGSVQ----YALNDNAGRH 172

Query: 195 ------LELGGQFTSRGLSASAVLHRSH-GTAQGGADRSAQRRDIGTFAARYAFASLPLT 247
+ + R H ++ R + + +AS+ +
Sbjct: 173 NSESYHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIEKYQIHRLVSGYDNDALYASVAVQ 232

Query: 248 VHAGVQRLTGELDPARTIV-------WGGARYQASGRFGFAGGIYHTDSPTPQVGHPTLF 300
++T V +G + S GF G T+
Sbjct: 233 QQDAKLVEENYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDATNYNN----DYDQV 288

Query: 301 IASTTCSLSKRTVAYLNLGYAKN 323
+ SKRT A ++ G+ +
Sbjct: 289 VVGAEYDFSKRTSALVSAGWLQE 311


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2374TCRTETA310.009 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 30.9 bits (70), Expect = 0.009
Identities = 38/135 (28%), Positives = 57/135 (42%), Gaps = 4/135 (2%)

Query: 254 AQTSGNVLAIASLMGIAGAALASYLGGRAARRAMLLAGYGILAASLVALAAAPNANGYTL 313
G +LA+ +LM A A + L R RR +LL A +A AP +
Sbjct: 42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYI 101

Query: 314 A--IFGFKFAWTFVLPFMLASVAAVDATGRLIATLNLVIGSGLAAGPLAAGLMLDGGGTL 371
+ G A V +A + D R ++ G G+ AGP+ GLM GG +
Sbjct: 102 GRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLM--GGFSP 159

Query: 372 RALFSIAAAVSLVSL 386
A F AAA++ ++
Sbjct: 160 HAPFFAAAALNGLNF 174


33BURPS1106A_2437BURPS1106A_2451Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_24373142.615592cytidine/deoxycytidylate deaminase family
BURPS1106A_24383201.192719hypothetical protein
BURPS1106A_24394211.351772hypothetical protein
BURPS1106A_2440330-1.209666hypothetical protein
BURPS1106A_2441636-4.888110hypothetical protein
BURPS1106A_2442741-5.418775major facilitator family transporter
BURPS1106A_24431050-7.789584hypothetical protein
BURPS1106A_24441051-7.882322hypothetical protein
BURPS1106A_24451053-8.466166hypothetical protein
BURPS1106A_2446951-9.727724hypothetical protein
BURPS1106A_2447748-10.788405hypothetical protein
BURPS1106A_2448747-10.958113hypothetical protein
BURPS1106A_2449222-6.839230hypothetical protein
BURPS1106A_2450223-6.745839hypothetical protein
BURPS1106A_2451218-4.198872S-adenosylhomocysteine hydrolase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2442TCRTETA445e-07 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 44.4 bits (105), Expect = 5e-07
Identities = 76/334 (22%), Positives = 117/334 (35%), Gaps = 13/334 (3%)

Query: 58 ALALLLLVP-LGDLVDR--RRLMLVQSLALAATLIAV-GFASASAVLIAGMLGTGLLGTA 113
AL P LG L DR RR +L+ SLA AA A+ A VL G + G+ G
Sbjct: 53 ALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGAT 112

Query: 114 MTQGLVSYAASASASHERGRVVGAAQGGVVIGLLLARVLAGFVGDVAGWRGVYFLSAATM 173
+Y A + ER R G G++ VL G +G + + AA +
Sbjct: 113 GA-VAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFA--AAAL 169

Query: 174 LALAALLARKLPALAPASPRIGYPRLIASLFGLLRDERVLQIRGMLAMLMFAA--FNIFW 231
L L L + R R + R R + + L + F
Sbjct: 170 NGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVP 229

Query: 232 SALALPLSAPPYTLSHTAIG-AFGLVGALGAFAAARAGHWADRGFGQPTSAAALALLLAS 290
+AL + + T IG + G L + A A G+ + + +
Sbjct: 230 AALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGT 289

Query: 291 WLPLAFMPMSLWALVLGIVLLDAGGQAIHVTNQSMIFRARPDAHSRLIAAYMLFYSVGSG 350
L W +VLL +GG + + + + +L + S+ S
Sbjct: 290 GYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSI 349

Query: 351 LGAIASTAVYATH--GWRG-VCMLGAAVSAAALI 381
+G + TA+YA W G + GAA+ L
Sbjct: 350 VGPLLFTAIYAASITTWNGWAWIAGAALYLLCLP 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2446BCTERIALGSPF310.015 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 31.3 bits (71), Expect = 0.015
Identities = 36/160 (22%), Positives = 49/160 (30%), Gaps = 16/160 (10%)

Query: 59 AHSQAQLAQLEGRVNLYRQYQAKLREREFLATEAIPVLTWYEERQDAAIKLRELHRQRLH 118
A + E RQ + LRER + ++ + LR R
Sbjct: 11 AQGKKCRGTQEADSA--RQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLRRKIRLSTS 68

Query: 119 Q-AQLKRQLAADSAMLLQLLEQKRIAAE------------RVRQFEAE-RDAALALQRQA 164
A L RQLA A + L E A+ VR E A A++
Sbjct: 69 DLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSLADAMKCFP 128

Query: 165 RDVERPVEDLVKHAEELQTLATVETNAADLAEHLRVLEQR 204
ER +V E L V AD E + + R
Sbjct: 129 GSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSR 168


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2447PYOCINKILLER270.027 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 27.1 bits (59), Expect = 0.027
Identities = 25/113 (22%), Positives = 49/113 (43%), Gaps = 4/113 (3%)

Query: 18 ARAASADGETQAEMFAGDRPMPSAVSVSPLVSYKALLADFGTQLGKKT-RMDVNLKRLET 76
+ QAE+ D + A +++PL L G L +K ++ +N K++ +
Sbjct: 84 DAEKKLEASVQAELDKADAALGPAKNLAPLDVINRSLTIVGNALQQKNQKLLLNQKKITS 143

Query: 77 HG---FITRRGDDIAEGPLLDLLLDYNALAPRILDGALSDVLARTRSESATES 126
G F+TR ++I E + + ++ R LD + + A + TE+
Sbjct: 144 LGAKNFLTRTAEEIGEQAVREGNINGPEAYMRFLDREMEGLTAAYNVKLFTEA 196


34BURPS1106A_2572BURPS1106A_2578Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_25721113.116718hypothetical protein
BURPS1106A_25731103.432677dioxygenase, TauD/TfdA
BURPS1106A_25741103.747686hypothetical protein
BURPS1106A_2575184.051751non-ribosomal peptide synthase
BURPS1106A_25762104.265330hypothetical protein
BURPS1106A_2577-1114.366797hypothetical protein
BURPS1106A_2578-1104.011836major facilitator family transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2578TCRTETA385e-05 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 38.3 bits (89), Expect = 5e-05
Identities = 57/271 (21%), Positives = 97/271 (35%), Gaps = 13/271 (4%)

Query: 74 AFTLPIALFALLSGVAADAWDRRTVMLLSQALMFSVALCLVALAAAGAMTPARLLVCMFV 133
+ L A + G +D + RR V+L+S +V ++A A + L + V
Sbjct: 51 LYALMQFACAPVLGALSDRFGRRPVLLVS-LAGAAVDYAIMATAPFLWV----LYIGRIV 105

Query: 134 GGCAGAMFQPAWQSAVTEQVPARELSAAIALDSFSMNFARTAGPALGGFIVASVSPNAAF 193
G GA + + + E + S F AGP LGG + SP+A F
Sbjct: 106 AGITGATG-AVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLM-GGFSPHAPF 163

Query: 194 V---LSGLSYAGLIYALSRSIRGAAARPPVRERLATMLVQGVRYCGRARGIRGTLIRSSL 250
L RP R A + R+ + + +
Sbjct: 164 FAAAALNGLNFLTGCFLLPESHKGERRP--LRREALNPLASFRWARGMTVVAALMAVFFI 221

Query: 251 FGFLGSPVWALLPLFAKTQFGGEARTYGVLLASFGA-GAASGALGGAAGRARLGREALVR 309
+G AL +F + +F +A T G+ LA+FG + + A+ ARLG +
Sbjct: 222 MQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM 281

Query: 310 LCTLTFAAGMLATAWSPCQAVAMLGLAVAGG 340
L + G + A++ +A + +
Sbjct: 282 LGMIADGTGYILLAFATRGWMAFPIMVLLAS 312



Score = 35.2 bits (81), Expect = 5e-04
Identities = 31/167 (18%), Positives = 58/167 (34%), Gaps = 8/167 (4%)

Query: 21 LAALRGPFAYRTFAAIWVAS-LVGNIGGSIQTVAASWLMTSMAPSPTMVSLVQTAFTLPI 79
LA+ R AA+ ++ +G + + T + + AF +
Sbjct: 200 LASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILH 259

Query: 80 ALF-ALLSGVAADAWDRRTVMLLSQALMFSVALCLVALAAAGAMTPARLLVCMFVGGCAG 138
+L A+++G A R ++L M + + LA A A ++ + G
Sbjct: 260 SLAQAMITGPVAARLGERRALMLG---MIADGTGYILLAFATRGWMAFPIMVLLASG--- 313

Query: 139 AMFQPAWQSAVTEQVPARELSAAIALDSFSMNFARTAGPALGGFIVA 185
+ PA Q+ ++ QV + + GP L I A
Sbjct: 314 GIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYA 360


35BURPS1106A_2704BURPS1106A_2715Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2704114-4.320618LysR family transcriptional regulator
BURPS1106A_2706013-4.829330*hypothetical protein
BURPS1106A_2707-211-2.563991transposase B
BURPS1106A_2708-410-1.539384transposase A
BURPS1106A_2709-410-0.673847hypothetical protein
BURPS1106A_2710-310-0.130281aminotransferase
BURPS1106A_27110120.374335glutamine synthetase
BURPS1106A_27123143.676121glutamine amidotransferase
BURPS1106A_27134123.250554hypothetical protein
BURPS1106A_27143113.185911hypothetical protein
BURPS1106A_27153113.576720hypothetical protein
36BURPS1106A_2889BURPS1106A_2898Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_28895124.009737hypothetical protein
BURPS1106A_28902102.833201hypothetical protein
BURPS1106A_2891082.384561hypothetical protein
BURPS1106A_2892091.831023ArsR family transcriptional regulator
BURPS1106A_2893091.301865hypothetical protein
BURPS1106A_28941101.591040hypothetical protein
BURPS1106A_2895219-0.705582thymidylate synthase
BURPS1106A_28962190.666053sigma-54 dependent trancsriptional regulator
BURPS1106A_28973221.110332hypothetical protein
BURPS1106A_28982160.388251hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2892adhesinmafb320.002 Neisseria meningitidis: adhesin MafB signature.
		>adhesinmafb#Neisseria meningitidis: adhesin MafB signature.

Length = 467

Score = 32.0 bits (72), Expect = 0.002
Identities = 17/67 (25%), Positives = 28/67 (41%), Gaps = 3/67 (4%)

Query: 36 SARPAGELTMIAGLSPSAASAHLARLTDGGLLAL---DVRGRHRYYRIATPDIAAAIEAL 92
R A + + ++P A A + G +A + R + P+ A +EA+
Sbjct: 254 GTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEAV 313

Query: 93 ANVAQAA 99
NVA AA
Sbjct: 314 FNVAAAA 320


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2894IGASERPTASE436e-06 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 42.7 bits (100), Expect = 6e-06
Identities = 42/263 (15%), Positives = 63/263 (23%), Gaps = 12/263 (4%)

Query: 583 NPAARAGERPQPNMPQPNAAQPNAAQPNIARPGQPQPGVAQPTAPHAPGTPPNAMRPDAA 642
NP + + N PN Q P P AP PP P
Sbjct: 982 NPEVEKRNQT---VDTTNITTPNNIQ--ADVPSVPSNNEEIARVDEAPVPPPAPATPSET 1036

Query: 643 RPNEARPAPAPSARNGVPRPPAAVENPGMRDEARAPGEAPRPQPSWTQPHPPIQQQRANE 702
A + S A R+ A+ EA + TQ + Q +
Sbjct: 1037 TETVAENSKQESKTVEKNEQDATETTAQNREVAK---EAKSNVKANTQTNEVAQSGSETK 1093

Query: 703 GGPRASGEPNAPLNYRSPTQNALPPIRSTPTPTHSAPPAPPPAERAQPQPQPGPAPRNAM 762
+ A + + + P T P +E QPQ +P +
Sbjct: 1094 ETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTV 1153

Query: 763 RAPEAPRQEVAPPAPRNEYRAPAPAPRPQIE--APRMEAPRMP-APRAEAP-RMEPRPAP 818
E Q + + + + P P +P
Sbjct: 1154 NIKEPQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNS 1213

Query: 819 PPPAVPHNPPPAPRQEPPHQARP 841
P N + PH P
Sbjct: 1214 ESSNKPKNRHRRSVRSVPHNVEP 1236


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2896HTHFIS376e-129 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 376 bits (968), Expect = e-129
Identities = 133/388 (34%), Positives = 202/388 (52%), Gaps = 40/388 (10%)

Query: 101 FDYVTVPYECDRIVESVGHAYGMVTLSEGLAPAAATVRNEGEMVGTCEAMLALFKMIRKV 160
+DY+ P++ ++ +G A + + ++ +VG AM +++++ ++
Sbjct: 99 YDYLPKPFDLTELIGIIGRA--LAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARL 156

Query: 161 ASTDAPVFISGESGTGKELTAVAIHERSSRAGAPFVAINCGAIPPTLLQAELFGYERGAF 220
TD + I+GESGTGKEL A A+H+ R PFVAIN AIP L+++ELFG+E+GAF
Sbjct: 157 MQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAF 216

Query: 221 TGANQRKIGRIEAANGGTLFLDEIGDLPFESQASLLRFLQEHKVERVGGHQSIPVDVRII 280
TGA R GR E A GGTLFLDEIGD+P ++Q LLR LQ+ + VGG I DVRI+
Sbjct: 217 TGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIV 276

Query: 281 SATHVDMQIALRNGRFREDLYHRLCVLKLEEPPLRERGKDIEILARHMLERFKGDAHRRL 340
+AT+ D++ ++ G FREDLY+RL V+ L PPLR+R +DI L RH +++ + + +
Sbjct: 277 AATNKDLKQSINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEG-LDV 335

Query: 341 RGFTPDAIAALHNYAWPGNVRELINRVRRAIVMSEGRMISAADLELSGYAEVA------- 393
+ F +A+ + + WPGNVREL N VRR + +I+ +E +E+
Sbjct: 336 KRFDQEALELMKAHPWPGNVRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKA 395

Query: 394 ------------------------------PMSLEEARESAERHAIEVALLRHRGRLADA 423
+ E I AL RG A
Sbjct: 396 AARSGSLSISQAVEENMRQYFASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKA 455

Query: 424 ARELGVSRVTLYRLLCAYGMRDDDGARA 451
A LG++R TL + + G+ +R+
Sbjct: 456 ADLLGLNRNTLRKKIRELGVSVYRSSRS 483


37BURPS1106A_2940BURPS1106A_2958Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2940012-3.091246rfaE protein
BURPS1106A_2941010-3.563263UDP-glucose 6-dehydrogenase
BURPS1106A_2942010-1.693027tetratricopeptide repeat protein
BURPS1106A_2943010-1.315985hypothetical protein
BURPS1106A_294409-1.588880integration host factor subunit beta
BURPS1106A_2945011-1.71177030S ribosomal protein S1
BURPS1106A_2946-19-1.185489cytidylate kinase
BURPS1106A_2947-110-1.424418bifunctional prephenate
BURPS1106A_2948-110-3.363971chorismate mutase/prephenate dehydratase
BURPS1106A_2949-113-3.704590phosphoserine aminotransferase
BURPS1106A_2950-214-2.507129hypothetical protein
BURPS1106A_2951-314-2.244515DNA gyrase subunit A
BURPS1106A_2952118-0.371206hypothetical protein
BURPS1106A_2953016-0.330989ompA family protein
BURPS1106A_29540111.9719643-demethylubiquinone-9 3-methyltransferase
BURPS1106A_29552141.649422phosphoglycolate phosphatase
BURPS1106A_29572171.152907hypothetical protein
BURPS1106A_29583142.155349phospholipid N-methyltransferase PmtA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2944DNABINDINGHU1098e-35 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 109 bits (273), Expect = 8e-35
Identities = 35/89 (39%), Positives = 58/89 (65%), Gaps = 1/89 (1%)

Query: 2 TKSELVAQLASRFPQLVLKDADFAVKTMLDAMSDALSKGHRIEIRGFGSFGLNRRPARVG 61
K +L+A++A +L KD+ AV + A+S L+KG ++++ GFG+F + R AR G
Sbjct: 3 NKQDLIAKVAEA-TELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKG 61

Query: 62 RNPKSGEKVQVPEKHVPHFKPGKELRERV 90
RNP++GE++++ VP FK GK L++ V
Sbjct: 62 RNPQTGEEIKIKASKVPAFKAGKALKDAV 90


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2953OMPADOMAIN1684e-53 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 168 bits (426), Expect = 4e-53
Identities = 75/146 (51%), Positives = 99/146 (67%), Gaps = 3/146 (2%)

Query: 74 AQAPAPAPVAPVAPAITSQKITYQADTLFDFDKAVLKPAGKQKLDELAAKIQGMNVE--V 131
AP AP AP + ++ T ++D LF+F+KA LKP G+ LD+L +++ ++ +
Sbjct: 195 EAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGS 254

Query: 132 VVATGYTDRIGSDKYNDRLSLRRAQAVKSYLVSKGVPANKVYTEGKGKRNPVTGNTC-KQ 190
VV GYTDRIGSD YN LS RRAQ+V YL+SKG+PA+K+ G G+ NPVTGNTC
Sbjct: 255 VVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNV 314

Query: 191 KNRKQLIACLAPDRRVEVEVVGTQEV 216
K R LI CLAPDRRVE+EV G ++V
Sbjct: 315 KQRAALIDCLAPDRRVEIEVKGIKDV 340


38BURPS1106A_2988BURPS1106A_3009Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2988335-2.502356hypothetical protein
BURPS1106A_2990332-2.227318*hypothetical protein
BURPS1106A_2991430-2.241894hypothetical protein
BURPS1106A_2992228-1.095421hypothetical protein
BURPS1106A_29932200.948011hypothetical protein
BURPS1106A_2994-1100.557972hypothetical protein
BURPS1106A_2995-1101.090192hypothetical protein
BURPS1106A_29960111.528955hypothetical protein
BURPS1106A_29970101.412764hypothetical protein
BURPS1106A_2998191.015693hypothetical protein
BURPS1106A_29992130.800222TonB-dependent receptor
BURPS1106A_30003281.649944hypothetical protein
BURPS1106A_30012182.046446hypothetical protein
BURPS1106A_30023180.076317hypothetical protein
BURPS1106A_3003318-0.086181hypothetical protein
BURPS1106A_3005615-1.429920chorismate mutase
BURPS1106A_3004515-2.666272hypothetical protein
BURPS1106A_3006-18-3.283498exonuclease DNA polymerase III subunit epsilon
BURPS1106A_3007-110-4.665030cold-shock domain-contain protein
BURPS1106A_3008-29-3.869702hypothetical protein
BURPS1106A_3009-49-3.108980outer membrane porin
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2999SURFACELAYER300.030 Lactobacillus surface layer protein signature.
		>SURFACELAYER#Lactobacillus surface layer protein signature.

Length = 439

Score = 30.4 bits (68), Expect = 0.030
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)

Query: 13 RLAAACAAALAWPAAHAASTAAAVPADSTPAAAAEMTASGKTLDTVKVT 61
R+ +A AAAL A A+TA V A +T A + + A+ V VT
Sbjct: 6 RIVSAAAAALL-AVAPIAATAMPVNAATTINADSAINANTNAKYDVDVT 53


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3009ECOLNEIPORIN1274e-36 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 127 bits (322), Expect = 4e-36
Identities = 89/386 (23%), Positives = 143/386 (37%), Gaps = 62/386 (16%)

Query: 1 MKKTLIVAALSGVFATAAHAQSSVTLYGLIDAGITYTNNQGGHSAWS-----QSTGSVNG 55
MKK+LI L+ + A + VTLYG I AG+ + + + A + + G
Sbjct: 1 MKKSLIALTLAALPVAAM---ADVTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLG 57

Query: 56 SRWGLRGAEDLGGGLKAIFVLENGFGINNGTLKQNGREFGRQAFVGLSHEQYGALTLGRQ 115
S+ G +G EDLG GLKAI+ +E I + RQ+F+GL +G L +GR
Sbjct: 58 SKIGFKGQEDLGNGLKAIWQVEQKASIAGT----DSGWGNRQSFIGLK-GGFGKLRVGRL 112

Query: 116 YDSVVDYLG--PLSLTGTQFGGTQFAHPFDNDNLNNSFRINNAVKYTSVNWAGLKFGALY 173
+ D P G + A P + S V+Y S +AGL Y
Sbjct: 113 NSVLKDTGDINPWDSKSDYLGVNKIAEP---EARLIS------VRYDSPEFAGLSGSVQY 163

Query: 174 GFSNNNQFANNRAYSAGVSYSYAGFNIGAGYLQLNNNFGPTVSNASGAVALDNTFVGKRQ 233
++N N+ +Y AG +Y GF + G ++ ++
Sbjct: 164 ALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHHQV------------QENVNIEKY 211

Query: 234 RVFGGGLNYTFGPATAGFVFTQSRVNRATAIGAGASGVSSGIALDGTFMRFNNYEVNARY 293
++ Y A + + A + S S RF N
Sbjct: 212 QIHRLVSGYD---NDALYASVAVQQQDAKLVEENYSHNSQTEVAATLAYRFG----NVTP 264

Query: 294 AITPAWTVAGSYTYTAGFIENHHPGWNQFNLQTAYALSKRTDVYLQGVYQKVNNDGTGLG 353
++ A GS+ T N++ ++Q + Y SKRT + + + +G G
Sbjct: 265 RVSYAHGFKGSFDAT-----NYNNDYDQVVVGAEYDFSKRTSALVSAGWLQ---EGKGES 316

Query: 354 AYINGIGGMSSTEKQIAVTAGLRHRF 379
++ A GLRH+F
Sbjct: 317 KFV-----------STAGGVGLRHKF 331


39BURPS1106A_3125BURPS1106A_3137Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3125223-4.563693glycoside hydrolase family protein
BURPS1106A_3126234-5.719228hypothetical protein
BURPS1106A_3127135-6.589275capsular polysaccharide biosynthesis protein
BURPS1106A_3128241-7.787921glycoside hydrolase family protein
BURPS1106A_3129139-7.682809NAD-dependent epimerase/dehydratase family
BURPS1106A_3130241-9.071960glycosyl transferase family protein
BURPS1106A_3131241-8.922563glycosyl transferase family protein
BURPS1106A_3132340-9.712552glycosyl transferase family protein
BURPS1106A_3133440-9.252655glycosyl transferase family protein
BURPS1106A_3134541-9.576148NAD-dependent epimerase/dehydratase family
BURPS1106A_3135437-9.250435O-antigen acetylase WbiA
BURPS1106A_3136229-7.556034lipopolysaccharide ABC transporter ATP-binding
BURPS1106A_3137123-5.483098lipopolysaccharide ABC transporter permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3127NUCEPIMERASE728e-16 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 72.1 bits (177), Expect = 8e-16
Identities = 53/301 (17%), Positives = 108/301 (35%), Gaps = 50/301 (16%)

Query: 288 VMVTGAGGSIGSELCRQILKFQPAQLIAFD-LSEYAMYRLTEELRERFPDLPVVPIIGDA 346
+VTGA G IG + +++L+ Q++ D L++Y L + E D
Sbjct: 3 YLVTGAAGFIGFHVSKRLLE-AGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 347 KDSLLLDQVMSRYAPHIVFHAAAYKHVPLMEELNAWQALRNNVLGTYRVARAAIRHDVRH 406
D + + + VF + V E N +N+ G + + ++H
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLE-NPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 407 FVLIST---------------DKAVNPTNVMGASKRLAE-MACQALQQTSARTQFETV-- 448
+ S+ D +P ++ A+K+ E MA S
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTY----SHLYGLPATGL 176

Query: 449 RFGNVLGSAGS---VIPKFQQQIAKGGPVTV-THPEITRFFMTIPEASQLVLQA------ 498
RF V G G + KF + + +G + V + ++ R F I + ++ +++
Sbjct: 177 RFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPH 236

Query: 499 ------------SSMGQGGEIFILDMGEPVKIVDLARDLIRLYGFTEEQIRIEFSGLRPG 546
++ ++ + PV+++D + L G + + L+PG
Sbjct: 237 ADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGI---EAKKNMLPLQPG 293

Query: 547 E 547
+
Sbjct: 294 D 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3129NUCEPIMERASE1061e-28 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 106 bits (267), Expect = 1e-28
Identities = 67/344 (19%), Positives = 129/344 (37%), Gaps = 42/344 (12%)

Query: 3 RVIVTGANGFVGRALCRALLAAGHEVTGL-------------VRRRGVCTEGVSEWVHEA 49
+ +VTGA GF+G + + LL AGH+V G+ R + G H+
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQ--FHKI 59

Query: 50 D--DFDGVADRWPAGLQVDAVVHLAARVHMMRDRSPDPDAAFRASNVAATMRVARAARQQ 107
D D +G+ D + +G + V R+ + S + A+ SN+ + + R
Sbjct: 60 DLADREGMTDLFASG-HFERVFISPHRLAV--RYSLENPHAYADSNLTGFLNILEGCRHN 116

Query: 108 GARRFVFLS--SVKAIAESDGGTPLCENSMPA-PQDAYGRSKLEAERALEQLRDELSFDT 164
+ ++ S SV + P + P Y +K E
Sbjct: 117 KIQHLLYASSSSVYGLNRKM---PFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPA 173

Query: 165 VIVRPPLVYGPGVRAN--FLSLMRAVSRGVPLPL-GAVRARRSMVYVDNLADAVMRCVTE 221
+R VYGP R + +A+ G + + + +R Y+D++A+A++R
Sbjct: 174 TGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDV 233

Query: 222 PAATNGCFHVADSDMPPTIAEL-LDDIGHHLGRPARLLPVPERLLRVAGALTGRAAQ--- 277
+ + V +IA + +IG+ P L+ ++ G A+
Sbjct: 234 IPHADTQWTVETGTPAASIAPYRVYNIGN--SSPVELM----DYIQALEDALGIEAKKNM 287

Query: 278 IDRLTSDLR---LDTTHIRTVLDWRPPRSSEEGLAETACWFKSL 318
+ D+ DT + V+ + P + ++G+ W++
Sbjct: 288 LPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDF 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3134NUCEPIMERASE1682e-51 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 168 bits (426), Expect = 2e-51
Identities = 83/363 (22%), Positives = 136/363 (37%), Gaps = 58/363 (15%)

Query: 6 KILVTGGAGFIGCAISERLAARASRYVVMDNLHPQIHASAVRPGALHEKAE----LVVAD 61
K LVTG AGFIG +S+RL + V +DNL+ + +++ L A+ D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDY-YDVSLKQARLELLAQPGFQFHKID 60

Query: 62 VTDAGAWDALLSDFQPEIIIHLAAETGTGQSLTEASRHALVNVVGTTRLTDALVKHGIVV 121
+ D L + E + SL +A N+ G + + + +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK--I 118

Query: 122 EHILLTSSRAVYGEGAWQKDDGTIVYPGQRGRAQLEAAQWDFPGMTMLPSRADRTEPRPT 181
+H+L SS +VYG +P D + P
Sbjct: 119 QHLLYASSSSVYGLN------------------------------RKMPFSTDDSVDHPV 148

Query: 182 SVYGATKLAQEHVLRAWSLATKTPLSILRLQNVYGPGQSLTNSYTGIVALFSRLAREKKV 241
S+Y ATK A E + +S P + LR VYGP + F++ E K
Sbjct: 149 SLYAATKKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMALF----KFTKAMLEGKS 204

Query: 242 IPLYEDGNVTRDFVSIDDVADAIVATLVRTPEA-----------------LSLFDIGSGQ 284
I +Y G + RDF IDD+A+AI+ P A +++IG+
Sbjct: 205 IDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSS 264

Query: 285 ATSILDMARIIAAHYGAPEPQINGAFRDGDVRHAACDLSESLANLGWKPQWSLKRGIGEL 344
++D + + G + + GDV + D +G+ P+ ++K G+
Sbjct: 265 PVELMDYIQALEDALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNF 324

Query: 345 QTW 347
W
Sbjct: 325 VNW 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3137ABC2TRNSPORT300.007 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 30.3 bits (68), Expect = 0.007
Identities = 16/59 (27%), Positives = 24/59 (40%)

Query: 195 LFTMVLMFLSPVFYPASALPEKYRFWLELNPLTLFIEQSRGILLEGRVPDFHPLGLAFL 253
L ++FLS +P LP ++ PL+ I+ R I+L V D A
Sbjct: 184 LVITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALC 242


40BURPS1106A_3151BURPS1106A_3168Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3151126-4.562995rubredoxin
BURPS1106A_3152127-3.780429phosphomethylpyrimidine kinase
BURPS1106A_3153731-2.804446molecular chaperone GroEL
BURPS1106A_31541137-2.065534co-chaperonin GroES
BURPS1106A_31561240-3.047900hypothetical protein
BURPS1106A_31551240-3.290923hypothetical protein
BURPS1106A_31571240-3.475296hypothetical protein
BURPS1106A_31581239-3.889749hypothetical protein
BURPS1106A_3159434-5.467530hypothetical protein
BURPS1106A_3161429-3.140447hypothetical protein
BURPS1106A_3160326-0.965387hypothetical protein
BURPS1106A_3162123-0.580200hypothetical protein
BURPS1106A_3163019-0.662160transcriptional regulator
BURPS1106A_3164015-0.716888zinc-binding dehydrogenase family
BURPS1106A_3165112-1.282586hypothetical protein
BURPS1106A_3167314-2.517860hypothetical protein
BURPS1106A_3166314-3.326158hypothetical protein
BURPS1106A_3168213-3.531256OmpW family outer membrane protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3158PYOCINKILLER300.038 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 30.1 bits (67), Expect = 0.038
Identities = 26/79 (32%), Positives = 35/79 (44%), Gaps = 2/79 (2%)

Query: 498 ANALSVANPAALTAAANTVAGTLARAANGTPVAGAIGGLVAALPVANPAGALTSAANNAA 557
A A A A AA A T A ANG+ VA A G + VA A +L A ++A
Sbjct: 228 AEAKRKAEEQARQQAAIRAANTYAMPANGSVVATAAGR--GLIQVAQGAASLAQAISDAI 285

Query: 558 STIATVAGTNPAAAIGGVA 576
+ + V + P+ G A
Sbjct: 286 AVLGRVLASAPSVMAVGFA 304


41BURPS1106A_3181BURPS1106A_3193Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3181213-0.886374carboxypeptidase C
BURPS1106A_3182190.758261hypothetical protein
BURPS1106A_3183090.944299hypothetical protein
BURPS1106A_3184-2102.186166alpha/beta hydrolase
BURPS1106A_3185-182.708606transport-associated domain-containing protein
BURPS1106A_3186083.032413hypothetical protein
BURPS1106A_3187183.3110612-hydroxychromene-2-carboxylate isomerase
BURPS1106A_3188294.1035542-hydroxychromene-2-carboxylate isomerase
BURPS1106A_3189394.529040transcriptional regulator
BURPS1106A_3190294.484835Fe3+-siderophore ABC transporter permease
BURPS1106A_3191194.031909membrane transport solute-binding protein
BURPS1106A_31920103.942946iron ABC transporter ATP-binding protein
BURPS1106A_3193093.007353Outer membrane receptor protein, mostly Fe
42BURPS1106A_3272BURPS1106A_3297Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3272539-7.168117sulfatase
BURPS1106A_3273436-7.676846short chain dehydrogenase/reductase family
BURPS1106A_3274536-9.265367capsule polysaccharide biosynthesis protein
BURPS1106A_3275437-9.373512D,D-heptose 1,7-bisphosphate phosphatase
BURPS1106A_3276438-10.403915D-glycero-D-manno-heptose 1-phosphate
BURPS1106A_3277439-10.877991phosphoheptose isomerase
BURPS1106A_3278339-11.180011D-glycero-D-manno-heptose 7-phosphate kinase
BURPS1106A_3279341-11.533267GDP-6-deoxy-D-lyxo-4-hexulose reductase
BURPS1106A_3280345-11.956494NAD-dependent epimerase/dehydratase family
BURPS1106A_3281447-12.560384capsular polysaccharide biosynthesis protein
BURPS1106A_3282547-12.542846glycoside hydrolase family protein
BURPS1106A_3283550-12.643824capsular polysaccharide biosynthesis protein
BURPS1106A_3284649-11.942760capsular polysaccharide biosynthesis protein
BURPS1106A_3286647-11.090356hypothetical protein
BURPS1106A_3285648-11.214030glycoside hydrolase family protein
BURPS1106A_3287642-8.288295capsular polysaccharide export inner-membrane
BURPS1106A_3288437-6.968725capsule polysaccharide exporter
BURPS1106A_3289230-5.220160capsular polysaccharide biosynthesis/export
BURPS1106A_3291026-4.542247glycoside hydrolase family protein
BURPS1106A_3290-121-4.044568hypothetical protein
BURPS1106A_3292-214-1.683764capsular polysaccharide biosynthesis protein
BURPS1106A_3293-110-0.469815mannose-1-phosphate guanylyltransferase
BURPS1106A_3294-180.965158glutamine amidotransferase
BURPS1106A_3295-181.137154small conductance mechanosensitive ion channel
BURPS1106A_3296-182.153593DedA family membrane protein
BURPS1106A_3297-1103.083469DNA mismatch repair protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3273DHBDHDRGNASE702e-16 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 70.5 bits (172), Expect = 2e-16
Identities = 66/249 (26%), Positives = 101/249 (40%), Gaps = 26/249 (10%)

Query: 12 ITGASAGLGRALARAYARPGVVLSLGGRDAVRLEESAADCRARGATVFVASIDVRDADAM 71
ITGA+ G+G A+AR A G ++ + +LE+ + +A DVRD+ A+
Sbjct: 13 ITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSAAI 72

Query: 72 R----RWLEQFDDAHPIHLLIANAGVASTLAHGGDWEARERTAAIVDTNFYGAMNAVLPV 127
R + PI +L+ AGV + E A N G NA V
Sbjct: 73 DEITARIEREMG---PIDILVNVAGVLRPGLI--HSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 128 IDRMRARGSGQVALISSLAALRGMAISPAYCASKAALKAWGDSVRPVLKRDGIRLSVVLP 187
M R SG + + S A AY +SKAA + + L IR ++V P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 188 GFVKTAMSDVFPADKPLLWSPDKAAQYIQRGIAARRAEIAFPALLALGMRLLPLL-PAVM 246
G +T M + LW+ + A+ + +G G+ L L P+ +
Sbjct: 188 GSTETDM-------QWSLWADENGAEQVIKGSLET---------FKTGIPLKKLAKPSDI 231

Query: 247 ADAILGRLS 255
ADA+L +S
Sbjct: 232 ADAVLFLVS 240


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3279NUCEPIMERASE1294e-37 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 129 bits (326), Expect = 4e-37
Identities = 80/352 (22%), Positives = 136/352 (38%), Gaps = 52/352 (14%)

Query: 4 RVLITGITGMVGSHLADFLLENTDWEIYGLCRWRSPLDNV-SHLLPRINEKNRIRL---- 58
+ L+TG G +G H++ LLE ++ G+ DN+ + + + L
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGH-QVVGI-------DNLNDYYDVSLKQARLELLAQPG 53

Query: 59 ---VYGDLRDYLSIHEAVKQSTPDFVFHLAAQSYPKTSFDSPLDTLETNVQGTANVLEAL 115
DL D + + + VF + + S ++P ++N+ G N+LE
Sbjct: 54 FQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGC 113

Query: 116 RKNNIDAVTHVCASSEVFGRVPREKLPIDEE-CTFHPASPYAISKVGTDLIGRYYAEAYN 174
R N I + +SS V+G K+P + HP S YA +K +L+ Y+ Y
Sbjct: 114 RHNKIQHLL-YASSSSVYGL--NRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170

Query: 175 MTVMTTRMFTHTGPR-RGDVFAESTFAKQIAMIERGLIPPVVKTGNLDSLRTFADVRDAV 233
+ R FT GP R D+ A F K + G V G + R F + D
Sbjct: 171 LPATGLRFFTVYGPWGRPDM-ALFKFTKAML---EGKSIDVYNYGKM--KRDFTYIDDIA 224

Query: 234 RAYYMLVTINPI-----------------PGAYYNIGGTYSCTVGQMLDTLISMSTSKDV 276
A L + P P YNIG + + + L +D
Sbjct: 225 EAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQAL------EDA 278

Query: 277 IRVETDPE--RLRPIDADLQVPNTRKFEAVTGWKPEISFEKTMEDLLNYWRA 326
+ +E L+P D +T+ V G+ PE + + +++ +N++R
Sbjct: 279 LGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRD 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3280NUCEPIMERASE451e-07 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 44.8 bits (106), Expect = 1e-07
Identities = 59/332 (17%), Positives = 105/332 (31%), Gaps = 82/332 (24%)

Query: 1 MKVFLVGSTGYIGKTLFDA-CSRRWRTLGT-STRDGADIVFSLARAEAFPYEQVSA--GD 56
MK + G+ G+IG + + +G + D D+ AR E D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 57 ------------------VVAVAA------AISSPDACAKDYETAFQVNVTGTLTLIRGV 92
V ++ +P A Y N+TG L ++ G
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHA----Y---ADSNLTGFLNILEG- 112

Query: 93 VARGA---RVIFFSSDTVYGASEQLLSEEAELT--PAGAYGAMKRRVEA---ELGENAAV 144
R +++ SS +VYG + ++ + P Y A K+ E +
Sbjct: 113 -CRHNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL 171

Query: 145 KVIRLSY--VFSLRDR-------FTQYLLGCAKEGKRADIFK--PFSRCVVYLSDVVEGV 193
L + V+ R FT+ +L EGK D++ R Y+ D+ E +
Sbjct: 172 PATGLRFFTVYGPWGRPDMALFKFTKAML----EGKSIDVYNYGKMKRDFTYIDDIAEAI 227

Query: 194 VSLIE-------RWD---------AIDERVINFVGPELVAREDFVEKIRNLAAPELDYGF 237
+ L + +W RV N V D+++ + + E
Sbjct: 228 IRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNM 287

Query: 238 SEP-EGDFFVNRPRIINVSSARFEKLLGRRPR 268
GD + + +++G P
Sbjct: 288 LPLQPGDVLETSA---DTKALY--EVIGFTPE 314


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3281PF05043300.007 Transcriptional activator
		>PF05043#Transcriptional activator

Length = 493

Score = 29.9 bits (67), Expect = 0.007
Identities = 19/76 (25%), Positives = 33/76 (43%), Gaps = 7/76 (9%)

Query: 48 RHLEEIGASLRIDIDE---IESWCVDELKSREVGENDGGKQIDISVTDFILANCRQKRLF 104
H + + +L +E W EL+ + D DI +++FI+ KRL
Sbjct: 414 YHAKFVAETLSYYCSNNFELEVW--TELELSKESLED--SPYDIIISNFIIPPIENKRLI 469

Query: 105 YTMNHPTAALMREIAA 120
Y+ N T +L+ + A
Sbjct: 470 YSNNINTVSLIYLLNA 485


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3287ABC2TRNSPORT382e-05 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 38.0 bits (88), Expect = 2e-05
Identities = 32/139 (23%), Positives = 58/139 (41%), Gaps = 7/139 (5%)

Query: 88 MAVTPNLALMYHRNVKVIDIFIARILLEVVGNTASFFVLMITFHALGLVDYPEDILEVMF 147
M M + +++ DI + + + + + ALG + +++
Sbjct: 94 MEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAALGYTQWLS----LLY 149

Query: 148 AWVMIIWFG---ASLGFIIGALSEKTELVEKLWHPVTYLMFPLSGAIFMVDWLSPAFQKI 204
A +I G ASLG ++ AL+ + V + LSGA+F VD L FQ
Sbjct: 150 ALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQTA 209

Query: 205 VLWLPMVHGVEMLREGYFG 223
+LP+ H ++++R G
Sbjct: 210 ARFLPLSHSIDLIRPIMLG 228


43BURPS1106A_3331BURPS1106A_3352Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3331-1103.346664glycolate oxidase FAD binding subunit
BURPS1106A_33320101.883348glycolate oxidase iron-sulfur subunit
BURPS1106A_3333092.385970hypothetical protein
BURPS1106A_33341112.477584pyrroline-5-carboxylate reductase
BURPS1106A_33352122.449493hypothetical protein
BURPS1106A_33362151.807236phosphonate ABC transporter permease
BURPS1106A_33372141.566674phosphonate ABC transporter periplasmic
BURPS1106A_33382142.602454phosphonate ABC transporter ATPase
BURPS1106A_33392172.828049phosphonate metabolism protein PhnM
BURPS1106A_33401162.809493phosphonate C-P lyase system protein PhnL
BURPS1106A_33411161.822240phosphonate C-P lyase system protein PhnK
BURPS1106A_33420122.005041phosphonate metabolism protein PhnJ
BURPS1106A_33432133.826764phosphonate metabolism protein PhnI
BURPS1106A_33443153.992647carbon-phosphorus lyase complex subunit
BURPS1106A_33452122.078869phosphonate metabolism protein PhnG
BURPS1106A_33461132.193783hypothetical protein
BURPS1106A_33470122.963992phosphonates metabolism transcriptional
BURPS1106A_3348-1122.321906hypothetical protein
BURPS1106A_3349-112-0.019175phosphonate metabolism
BURPS1106A_33500110.0785054-hydroxybenzoate octaprenyltransferase
BURPS1106A_33511101.262907transcriptional regulator
BURPS1106A_33522110.382930transcriptional regulatory protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3340PF05272290.017 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.017
Identities = 21/68 (30%), Positives = 28/68 (41%), Gaps = 1/68 (1%)

Query: 61 CVALTGPSGAGKSTLLRCLYGNYLANRGTIAVRVGTRAAEHVV-LTASEPHEVIALRRDV 119
V L G G GKSTL+ L G + + G + E + + A E E+ A RR
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSEMTAFRRAD 657

Query: 120 IGYVSQFL 127
V F
Sbjct: 658 AEAVKAFF 665


44BURPS1106A_3420BURPS1106A_3429Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3420327-0.281571hypothetical protein
BURPS1106A_3421228-0.837246hypothetical protein
BURPS1106A_3422128-1.419926hypothetical protein
BURPS1106A_3423219-4.302652HSP20 family protein
BURPS1106A_3424115-5.071263HSP20 family protein
BURPS1106A_3425211-5.128839chaperonin, 10 kDa
BURPS1106A_3426212-6.428665hypothetical protein
BURPS1106A_3427113-5.552465hypothetical protein
BURPS1106A_3428013-5.255492glutamate/aspartate ABC transporter ATP-binding
BURPS1106A_3429-213-3.503324glutamate/aspartate ABC transporter permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3427ACRIFLAVINRP250.039 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 25.2 bits (55), Expect = 0.039
Identities = 9/38 (23%), Positives = 21/38 (55%), Gaps = 1/38 (2%)

Query: 14 IEIDDVIVGLLAI-RLNLPENADPRDAISRHLSEAGGP 50
+ +DD IV + + R+ + + P++A + +S+ G
Sbjct: 404 LLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGA 441


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3428PF05272320.002 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 32.4 bits (73), Expect = 0.002
Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 5/53 (9%)

Query: 29 VVVVCGPSGSGKSTLIKTVNGLEPFQQGEILVNGQSVGDKKTNLSKLRSKVGM 81
VV+ G G GKSTLI T+ GL+ F +G K + ++ V
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHF-----DIGTGKDSYEQIAGIVAY 645


45BURPS1106A_3478BURPS1106A_3494Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_34783132.010985phosphatidylglycerophosphatase A
BURPS1106A_34794131.548095cinA family protein
BURPS1106A_34804131.377325hypothetical protein
BURPS1106A_34812120.904812orotidine 5'-phosphate decarboxylase
BURPS1106A_34822131.345005aldose 1-epimerase
BURPS1106A_34832131.145444NAD dependent epimerase/dehydratase family
BURPS1106A_34842141.970905L-arabinose transporter permease
BURPS1106A_34852142.625849L-arabinose transporter ATP-binding protein
BURPS1106A_34860132.911805carbohydrate ABC transporter periplasmic
BURPS1106A_3487-1131.552222short chain dehydrogenase
BURPS1106A_3488-1130.3525082-dehydro-3-deoxy-6-phosphogalactonate aldolase
BURPS1106A_3489015-0.9728522-dehydro-3-deoxygalactonokinase
BURPS1106A_3490115-2.687573IclR family transcriptional regulator
BURPS1106A_3491213-2.294613hypothetical protein
BURPS1106A_3492010-1.436172serine carboxypeptidase family protein
BURPS1106A_3494212-0.365626hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3478PF05616290.012 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 29.3 bits (65), Expect = 0.012
Identities = 14/38 (36%), Positives = 15/38 (39%)

Query: 4 DPTPRPADSADSASQPGATPAPASSPAPRRDSPQDPQR 41
+P RP D P A P P R DSP P R
Sbjct: 346 NPGTRPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDR 383



Score = 27.4 bits (60), Expect = 0.040
Identities = 12/24 (50%), Positives = 13/24 (54%)

Query: 7 PRPADSADSASQPGATPAPASSPA 30
PRP + SA P A P P SPA
Sbjct: 311 PRPDLTPGSAEAPNAQPLPEVSPA 334



Score = 27.4 bits (60), Expect = 0.049
Identities = 11/35 (31%), Positives = 14/35 (40%)

Query: 5 PTPRPADSADSASQPGATPAPASSPAPRRDSPQDP 39
P +P A P PAP +P R + DP
Sbjct: 323 PNAQPLPEVSPAENPANNPAPNENPGTRPNPEPDP 357


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3483DHBDHDRGNASE1243e-36 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 124 bits (311), Expect = 3e-36
Identities = 76/249 (30%), Positives = 113/249 (45%), Gaps = 8/249 (3%)

Query: 29 GRAVLITGGATGIGASFVEHFARQGARVAFVDLDEKAGRALVARLADAAHEPVFVVCDLT 88
G+ ITG A GIG + A QGA +A VD + + +V+ L A D+
Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67

Query: 89 DIGALRGAIDAIRVRIGPIAVLVNNAANDVRHAVADVTPESFDASIAVNLRHQFFAAQAV 148
D A+ I +GPI +LVN A + ++ E ++A+ +VN F A+++V
Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127

Query: 149 IDDMKRLGGGAIVNLGSIGWMLKNAGYPVYATAKAAVQGLTRALARELGPFGIRVNTLVP 208
M G+IV +GS + YA++KAA T+ L EL + IR N + P
Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187

Query: 209 GWVMTDKQRRLWLDDAGRAAIKAGQCIDAEL--------LPGDLARMALFLAADDSRLIT 260
G TD Q LW D+ G + G + P D+A LFL + + IT
Sbjct: 188 GSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHIT 247

Query: 261 AQDVVVDGG 269
++ VDGG
Sbjct: 248 MHNLCVDGG 256


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3485PF05272290.041 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.3 bits (65), Expect = 0.041
Identities = 14/38 (36%), Positives = 17/38 (44%), Gaps = 5/38 (13%)

Query: 18 RALD-GISFDVQAGQVHGLMGENGAGKSTLLKILGGEY 54
R ++ G FD L G G GKSTL+ L G
Sbjct: 587 RVMEPGCKFDY----SVVLEGTGGIGKSTLINTLVGLD 620


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3487DHBDHDRGNASE1321e-39 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 132 bits (333), Expect = 1e-39
Identities = 80/260 (30%), Positives = 129/260 (49%), Gaps = 14/260 (5%)

Query: 4 LAGKVAIVTGAGRGIGAAIARAFVREGAAVAIAELDAA---LAEESADAIARDTAGARVL 60
+ GK+A +TGA +GIG A+AR +GA +A + + S A AR
Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE----- 60

Query: 61 AVPTDVARAESVAAALARTERAFGLLDVLVNNAGVNVFGDPLALTDEDWRRCFAIDLDGV 120
A P DV + ++ AR ER G +D+LVN AGV G +L+DE+W F+++ GV
Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120

Query: 121 WNGCRAALPGMVERGRGSIVNIASTHAFKIIPGCFPYPVAKHGVLGLTRALGIEYAPRNV 180
+N R+ M++R GSIV + S A Y +K + T+ LG+E A N+
Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180

Query: 181 RVNAIAPGYIETQLTHDWWSAQPDPQAARRETLALQ-----PMKRIGRPDEVAMTAVFLA 235
R N ++PG ET + W+ + + + P+K++ +P ++A +FL
Sbjct: 181 RCNIVSPGSTETDMQWSLWADE-NGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

Query: 236 SDEAPFINASCITIDGGRSV 255
S +A I + +DGG ++
Sbjct: 240 SGQAGHITMHNLCVDGGATL 259


46BURPS1106A_3556BURPS1106A_3564Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3556-111-3.074466penicillin-binding protein
BURPS1106A_3557317-3.723031cell division protein FtsL
BURPS1106A_3558213-3.721158S-adenosyl-methyltransferase MraW
BURPS1106A_3559115-4.382944cell division protein MraZ
BURPS1106A_3560115-4.629355ubiquinone biosynthesis protein
BURPS1106A_3561216-5.163505outer membrane porin
BURPS1106A_3562219-5.481967transposase
BURPS1106A_3563017-4.700463long-chain-fatty-acid--CoA ligase
BURPS1106A_3564015-3.173953molybdopterin oxidoreductase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_355756KDTSANTIGN270.019 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 26.8 bits (59), Expect = 0.019
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 7/58 (12%)

Query: 24 NQQRQIFIQLQRAQSQEHQLQQDYAQLQYQQSA-------LSKTSRIEQLATSSLKMQ 74
NQ F+ +AQ Q+ Q QQ AQ Q++ L+ + +I QL +K+Q
Sbjct: 328 NQIHLNFVMPPQAQQQQGQGQQQQAQATAQEAVAAAAVRLLNGSDQIAQLYKDLVKLQ 385


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3561ECOLNEIPORIN881e-21 E.coli/Neisseria porin superfamily signature.
		>ECOLNEIPORIN#E.coli/Neisseria porin superfamily signature.

Length = 331

Score = 88.3 bits (219), Expect = 1e-21
Identities = 90/394 (22%), Positives = 139/394 (35%), Gaps = 71/394 (18%)

Query: 1 MKKSLLALVALSAFAGAAHAQSSVTLYGIIDEGFNINTNAGGKHL-----YNLSSGVMQG 55
MKKSL+AL L+A AA A VTLYG I G + + + V G
Sbjct: 1 MKKSLIALT-LAALPVAAMAD--VTLYGTIKAGVETSRSVAHNGAQAASVETGTGIVDLG 57

Query: 56 SRWGLRGTEDLGGGLKALFVLENGFDVNSGKLNQGGLEFGRQAYVGLSSGFGTVTLGRQY 115
S+ G +G EDLG GLKA++ +E + G RQ+++GL GFG + +GR
Sbjct: 58 SKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGN----RQSFIGLKGGFGKLRVGRLN 113

Query: 116 DSVVDF--VGPLEA-GDQWGGYIAAHPGDLDNFNNAYRVNNAVKFTSANYGGFTFGGLYS 172
+ D + P ++ D G A P + + V++ S + G + Y+
Sbjct: 114 SVLKDTGDINPWDSKSDYLGVNKIAEP---EARLIS------VRYDSPEFAGLSGSVQYA 164

Query: 173 FGGVAGDFSRNQTWSLGAGYTNGPLVLGVGYLNARTPSTAGGLFGNNTTSSTPAAVTTPV 232
AG ++++ G Y NG + G R + +
Sbjct: 165 LNDNAG-RHNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIEKYQIHR-------L 216

Query: 233 YAGYASAHTYQVIGAGGAYSFGAATVGITYSNIKFMNFASTVFPNQTATFNNAEINFKYQ 292
+GY + Y A A A + + + T + N +
Sbjct: 217 VSGYDNDALY----ASVAVQQQDAKL-VEENYSHNSQTEVAA----TLAYRFG--NVTPR 265

Query: 293 LTPTLLAGAAYDYTQGSKIAGSSAAKYHQGSVGVDYFLSKRTDVYAIGVYQHASGNVIEA 352
++ ++D T + Y Q VG +Y SKRT + E
Sbjct: 266 VSYAHGFKGSFDATNYNND-------YDQVVVGAEYDFSKRTSALVSAGWLQ------EG 312

Query: 353 DGNTVGPATAAINGLTPSSNRNQFAARVGIRHKF 386
G S A VG+RHKF
Sbjct: 313 KG---------------ESKFVSTAGGVGLRHKF 331


47BURPS1106A_3631BURPS1106A_3645Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_36312150.472929methyltransferase
BURPS1106A_3632014-2.483957hypothetical protein
BURPS1106A_3633-117-2.990160acyltransferase
BURPS1106A_3635019-3.029320hypothetical protein
BURPS1106A_3634119-4.318193lipoprotein
BURPS1106A_3636121-4.910512hypothetical protein
BURPS1106A_3637-116-3.165423M1 family peptidase
BURPS1106A_3638113-2.048607hypothetical protein
BURPS1106A_363929-1.269139hypothetical protein
BURPS1106A_364029-1.261503hypothetical protein
BURPS1106A_364109-0.245251hypothetical protein
BURPS1106A_3642111-1.431806secretion protein
BURPS1106A_3643114-1.546165toxin secretion ABC transporter ATP-binding
BURPS1106A_3644523-1.550960TolC family type I secretion outer membrane
BURPS1106A_3645210-1.406213hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3642RTXTOXIND1322e-36 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 132 bits (334), Expect = 2e-36
Identities = 81/429 (18%), Positives = 159/429 (37%), Gaps = 53/429 (12%)

Query: 28 RPVSFAVLASAAASMALGVI--LLFTFGTYTRRTTVDGVLTPDTGLVKVYAQQTGVVLKK 85
PVS A M VI +L G T +G LT ++ + +V +
Sbjct: 51 TPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEI 110

Query: 86 NVVEGQHVTRGQVLYTVSTDLQSAAAGQTQAAL----IEQAQQRKTSLQQELDKTRRLQ- 140
V EG+ V +G VL ++ A +TQ++L +EQ + + S EL+K L+
Sbjct: 111 IVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKL 170

Query: 141 ----------------------------QDERDTLQSKIASLRTELAGIDDQIAAQRTRA 172
Q+++ + + R E + +I +
Sbjct: 171 PDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLS 230

Query: 173 SIAADAASRYAGLLAQDYISKDQAQQRQADLLDQRSKLNSLMRDRASTAQSLKEALNDLS 232
+ ++ LL + I+K +++ ++ ++L + A +
Sbjct: 231 RVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQ 290

Query: 233 GLSLKQQNQLSQIDRSVIDVDRTLIESEAKREF-----VVTAPETGT-ATAVIAEPGQTA 286
++ +N++ R D L AK E V+ AP + + G
Sbjct: 291 LVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVV 350

Query: 287 DTSHPLASIVPTGAHWQAYLFVPSAAVGFVHVGDRVLVRYQAYPYQKFGQYEASVVSIAR 346
T+ L IVP + V + +GF++VG +++ +A+PY ++G V +I
Sbjct: 351 TTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINL 410

Query: 347 TALSAAELATSGGPAAQTASGTYYRITVALNSQNVMAYGRAQPLQAGMALQADVLQERRR 406
A+ G + + +++ + + PL +GMA+ A++ R
Sbjct: 411 DAIE------------DQRLGLVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMRS 458

Query: 407 LYEWVLEPL 415
+ ++L PL
Sbjct: 459 VISYLLSPL 467


48BURPS1106A_3655BURPS1106A_3706Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3655210-2.826787hypothetical protein
BURPS1106A_3656210-2.946686hypothetical protein
BURPS1106A_3657313-4.203158hypothetical protein
BURPS1106A_3658314-4.046728hypothetical protein
BURPS1106A_3659212-3.278552hypothetical protein
BURPS1106A_3660212-2.698159hypothetical protein
BURPS1106A_3661113-2.017893hypothetical protein
BURPS1106A_3662116-2.724689hypothetical protein
BURPS1106A_3663014-2.088254lipoprotein
BURPS1106A_3664-214-1.514651hypothetical protein
BURPS1106A_3665517-2.767609hypothetical protein
BURPS1106A_3666521-2.897563phage tail completion protein
BURPS1106A_3667626-4.653903DNA methylase
BURPS1106A_3668839-8.145871bacteriophage tail protein I
BURPS1106A_3669938-7.660826bacteriophage baseplate assembly protein J
BURPS1106A_3670834-6.731964ISBma1, transposase
BURPS1106A_3671840-7.428395hypothetical protein
BURPS1106A_3672740-7.784061gp31
BURPS1106A_3673739-7.101317hypothetical protein
BURPS1106A_3674637-6.450094hypothetical protein
BURPS1106A_3675536-6.358986helicase, C-terminal
BURPS1106A_3676641-7.248125hypothetical protein
BURPS1106A_3677538-6.862401helicase, C-terminal:dead/deah box helicase,
BURPS1106A_3678637-6.262475hypothetical protein
BURPS1106A_3679735-6.791093hypothetical protein
BURPS1106A_3680734-6.561229SNF2-like protein
BURPS1106A_3681937-7.178747modification methylase HgiDII
BURPS1106A_3682832-5.999497prophage CP4-57 regulatory protein (AlpA)
BURPS1106A_3683832-5.712529hypothetical protein
BURPS1106A_3684731-6.239528hypothetical protein
BURPS1106A_3685731-6.719518hypothetical protein
BURPS1106A_3686631-6.798415hypothetical protein
BURPS1106A_3687631-6.976159transposase, IS4
BURPS1106A_3688633-7.714741hypothetical protein
BURPS1106A_3689735-8.054010hypothetical protein
BURPS1106A_3690634-8.391586Type I restriction-modification system
BURPS1106A_3691634-7.729146XRE family transcriptional regulator
BURPS1106A_3692534-7.530199lipoprotein
BURPS1106A_3693435-7.825060lipoprotein
BURPS1106A_3694437-7.824090hypothetical protein
BURPS1106A_3695433-7.146616hypothetical protein
BURPS1106A_3696535-6.627038hypothetical protein
BURPS1106A_3697532-6.718616hypothetical protein
BURPS1106A_3698528-7.080938prophage CP4-57 regulatory protein (AlpA)
BURPS1106A_3699628-7.326039phage integrase
BURPS1106A_3700419-6.697102transposase, IS4
BURPS1106A_3701317-6.389780phage integrase
BURPS1106A_3703113-5.808330*ClpXP protease specificity-enhancing factor
BURPS1106A_370409-5.101165stringent starvation protein A
BURPS1106A_370519-4.134430ubiquinol-cytochrome c reductase, cytochrome c1
BURPS1106A_3706010-3.620046ubiquinol-cytochrome c reductase, cytochrome b
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3661IGASERPTASE290.012 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 29.3 bits (65), Expect = 0.012
Identities = 27/188 (14%), Positives = 58/188 (30%), Gaps = 10/188 (5%)

Query: 11 ASQPTPPTTEAFNKSLADADAVAKTGDQERAIGLYQQLAKSDPTREEPWSRIAQIQFQQG 70
Q P+ + N+ +A D A ++ +++E + Q
Sbjct: 1002 NIQADVPSVPSNNEEIARVDEAPVP-PPAPATPSETTETVAENSKQESKTVEKNEQDATE 1060

Query: 71 HYGQAIVAAQEALQRDKTDRQAKSVLAVAGLRIATESLGELRQDSSLAGDAKSDAQALAK 130
Q A+EA K + Q VA T+ + + + A+ +
Sbjct: 1061 TTAQNREVAKEAKSNVKANTQT---NEVAQSGSETKETQTTETKETATVEKEEKAKVETE 1117

Query: 131 QLRDTLGEAALFPPEQQATKPVVKKRRIVRRAKPVHEAPRAAESETAAAPATPPAAPAQP 190
+ ++ + P+Q+ + + +A+P E + + A QP
Sbjct: 1118 KTQEVPKVTSQVSPKQE------QSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQP 1171

Query: 191 AATPAPAP 198
A +
Sbjct: 1172 AKETSSNV 1179



Score = 28.1 bits (62), Expect = 0.032
Identities = 32/191 (16%), Positives = 60/191 (31%), Gaps = 32/191 (16%)

Query: 11 ASQPTPPTTEAFNKSLADADAVAKTGDQERAIGLYQQLAKSDPTREEPWSRIAQIQFQQG 70
+ T P N AD +V ++ I + P P +
Sbjct: 994 TTNITTP-----NNIQADVPSVPSNNEE---IARVDEAPVPPPAPATPSETTETV----- 1040

Query: 71 HYGQAIVAAQEALQRDKTDRQAKSVLAVAGLRIATESLGELRQDSSLAGDAKSDAQALAK 130
A + QE+ +K ++ A A +A E+ ++ ++ A+S ++
Sbjct: 1041 ----AENSKQESKTVEKNEQDATETTAQNR-EVAKEAKSNVKANTQTNEVAQSGSETKET 1095

Query: 131 QLRDTLGEAALFPPEQQATKPVVKKRRIVRRAKPVHEAPRAAESETAAAPATPPAAPAQP 190
Q E + T V K+ + + E P+ + +P + QP
Sbjct: 1096 Q-----------TTETKETATVEKEEKAKVETEKTQEVPKVT---SQVSPKQEQSETVQP 1141

Query: 191 AATPAPAPAKA 201
A PA
Sbjct: 1142 QAEPARENDPT 1152



Score = 27.7 bits (61), Expect = 0.039
Identities = 22/130 (16%), Positives = 41/130 (31%), Gaps = 22/130 (16%)

Query: 75 AIVAAQEALQRDKTDRQAKSVLAVAGLRIATESLGELRQDSSLAGDAKSDAQALAKQLRD 134
I A ++ + + V AT S ++A ++K +++ + K
Sbjct: 1002 NIQADVPSVPSNNEEIARVDEAPVPPPAPATPS----ETTETVAENSKQESKTVEKN--- 1054

Query: 135 TLGEAALFPPEQQATKPVVKKRRIVRRAKP-VHEAPRAAESETAAAPATPPAAPAQPAAT 193
EQ AT+ + R + + AK V + E A + Q T
Sbjct: 1055 ----------EQDATETTAQNREVAKEAKSNVKANTQTNE----VAQSGSETKETQTTET 1100

Query: 194 PAPAPAKAAG 203
A +
Sbjct: 1101 KETATVEKEE 1110


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3676PF05616340.002 Neisseria meningitidis TspB protein
		>PF05616#Neisseria meningitidis TspB protein

Length = 501

Score = 33.6 bits (76), Expect = 0.002
Identities = 22/66 (33%), Positives = 27/66 (40%), Gaps = 8/66 (12%)

Query: 28 PRKIDFEPPSSWLAGDRSIPKNSPARNPVQQSAPSGTVGV-------PAGKPSARPSRPV 80
PR D P S+ + +P+ SPA NP AP+ G P P A P
Sbjct: 311 PRP-DLTPGSAEAPNAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTDG 369

Query: 81 QPAFRP 86
QP RP
Sbjct: 370 QPGTRP 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3684BACINVASINB300.017 Salmonella/Shigella invasin protein B signature.
		>BACINVASINB#Salmonella/Shigella invasin protein B signature.

Length = 593

Score = 29.7 bits (66), Expect = 0.017
Identities = 19/70 (27%), Positives = 32/70 (45%)

Query: 183 PPADGSESADRALRHLYPGNGGTVDFTDDRRLSSVFADLVAVRAEIEARQSIEAQLKQTI 242
PP D + + L G + D LS + + L +A IE+++ + Q+ +
Sbjct: 70 PPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKEMGIQVSKEF 129

Query: 243 QQAMGEATHA 252
Q A+GEA A
Sbjct: 130 QTALGEAQEA 139


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3692INTIMIN300.033 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 30.0 bits (67), Expect = 0.033
Identities = 21/70 (30%), Positives = 29/70 (41%), Gaps = 1/70 (1%)

Query: 36 TTAITNTQLTGKAIDGYLAGATVCFDNGHGACDPSLPSTTTDASGNYTLNVSSNVTGKQL 95
T AIT T T K A V F+ G S S T+ SG T+ + S+ G+ +
Sbjct: 575 TEAITYT-ATVKKNGVAQANVPVSFNIVSGTAVLSANSANTNGSGKATVTLKSDKPGQVV 633

Query: 96 DVLVTSNTTD 105
T+ T
Sbjct: 634 VSAKTAEMTS 643


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3696GPOSANCHOR371e-04 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 37.0 bits (85), Expect = 1e-04
Identities = 20/63 (31%), Positives = 26/63 (41%), Gaps = 11/63 (17%)

Query: 201 KAKLNAQLAALDAETAEAKRREAEEDRAVAQVERD-----------DALRASLRADLVAS 249
KA L Q L+A +R A Q+E + +A R SLR DL AS
Sbjct: 297 KADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDAS 356

Query: 250 QIA 252
+ A
Sbjct: 357 REA 359



Score = 36.2 bits (83), Expect = 2e-04
Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 5/70 (7%)

Query: 199 VSKAKLNAQLAALDAETAEAKRREAEEDRAVAQVERDDALRASLRADLVAS-----QIAS 253
+S+A + LDA K+ EAE + Q + +A R SLR DL AS Q+
Sbjct: 341 ISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEK 400

Query: 254 LTGTTESQLE 263
S+L
Sbjct: 401 ALEEANSKLA 410


49BURPS1106A_3853BURPS1106A_3905Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3853212-0.591002hypothetical protein
BURPS1106A_38540110.312985tryptophan repressor binding protein
BURPS1106A_3855-1101.577981N-acetyl-gamma-glutamyl-phosphate reductase
BURPS1106A_3856-191.700873hypothetical protein
BURPS1106A_3857-3101.986017lipoprotein
BURPS1106A_3858-3102.466249ompW family protein
BURPS1106A_3859-192.979033LysR family transcriptional regulator
BURPS1106A_38600121.210267major facilitator transporter
BURPS1106A_3861318-1.416238LysE family translocator protein
BURPS1106A_3862422-2.218915dehydrogenase
BURPS1106A_3863530-3.490126hypothetical protein
BURPS1106A_3864641-5.959901hypothetical protein
BURPS1106A_3865643-6.335999hypothetical protein
BURPS1106A_3866738-4.271160hypothetical protein
BURPS1106A_3867539-4.284296hypothetical protein
BURPS1106A_3868432-2.650504lipoprotein
BURPS1106A_3869320-4.093517hypothetical protein
BURPS1106A_3870320-4.353777hypothetical protein
BURPS1106A_3871423-5.111650hypothetical protein
BURPS1106A_3872225-5.755305hypothetical protein
BURPS1106A_3873323-6.038876hypothetical protein
BURPS1106A_3874424-7.048651amino acid permease
BURPS1106A_3875927-6.682597hypothetical protein
BURPS1106A_3876928-6.966392hypothetical protein
BURPS1106A_3877928-6.957436transposase
BURPS1106A_3878929-6.910280transposase subfamily protein
BURPS1106A_3879930-7.052492outer membrane hemolysin activator protein
BURPS1106A_3880930-7.066324filamentous hemagglutinin/adhesin
BURPS1106A_3881846-9.648573hypothetical protein
BURPS1106A_3882749-10.111622transposase A
BURPS1106A_3883754-10.874454transposase B
BURPS1106A_3884751-10.682620hypothetical protein
BURPS1106A_3885750-10.301157transposase A
BURPS1106A_3886748-8.694056transposase B
BURPS1106A_3887747-8.560513hypothetical protein
BURPS1106A_3888645-7.726392hypothetical protein
BURPS1106A_3889642-6.519241transposase, IS4
BURPS1106A_3890746-7.547322hypothetical protein
BURPS1106A_3891642-6.864319helicase, C-terminal:dead/deah box helicase,
BURPS1106A_3892535-6.223260integrase/recombinase
BURPS1106A_3893432-5.435553hypothetical protein
BURPS1106A_3894227-3.646010hypothetical protein
BURPS1106A_3895118-1.875994hypothetical protein
BURPS1106A_3897-3111.149131*cytochrome c family protein
BURPS1106A_3898-1100.979545phospholipid-binding protein
BURPS1106A_38990100.817020phosphoheptose isomerase
BURPS1106A_39002130.087622hypothetical protein
BURPS1106A_3901113-1.366113tetrapyrrole methylase family protein
BURPS1106A_3902-213-2.468282rare lipoprotein A family protein
BURPS1106A_3903-114-3.462057metallo-beta-lactamase family protein
BURPS1106A_3904-119-4.730862hypothetical protein
BURPS1106A_3905-120-4.152485hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3880PF05860645e-14 haemagglutination activity domain.
		>PF05860#haemagglutination activity domain.

Length = 117

Score = 64.4 bits (157), Expect = 5e-14
Identities = 25/138 (18%), Positives = 49/138 (35%), Gaps = 23/138 (16%)

Query: 101 AQIVPTPGTSTH-VIQTPNGLPQVNVAAPSGAGVSVNTYNQFDVSRAGAILNNSATMVQT 159
AQI P + I T + +G+ + + + +F V +G N+ T
Sbjct: 1 AQITPDTTLPINSNITTEGNTRIIERGTQAGSNLFHS-FQEFSVPTSGTAFFNNPT---- 55

Query: 160 QQAGWINGNPNYSAGQAARIIVNQVNSPNPSQIRGALEIAGSRAELVLANPSGIYLDGAS 219
+ I+++V + S I G + A + A L L NP+GI +
Sbjct: 56 ----------------NIQNIISRVTGGSVSNIDGLIR-ANATANLFLINPNGIIFGQNA 98

Query: 220 FINTSRATLTTGVPYYGA 237
++ + + +
Sbjct: 99 RLDIGGSFVGSTANRLKF 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3893NUCEPIMERASE260.041 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 26.3 bits (58), Expect = 0.041
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 6/34 (17%)

Query: 5 MDLIREL--LLKLEA----LPMQPRDVAYIYSDV 32
MD I+ L L +EA LP+QP DV +D
Sbjct: 269 MDYIQALEDALGIEAKKNMLPLQPGDVLETSADT 302


50BURPS1106A_3933BURPS1106A_3949Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_39332121.174659chemotaxis protein CheR
BURPS1106A_39342130.927799methyl-accepting chemotaxis protein
BURPS1106A_3935213-0.496748chemotaxis protein CheW
BURPS1106A_3936213-0.324789chemotaxis protein CheA
BURPS1106A_3937012-2.535012chemotaxis protein CheY
BURPS1106A_3938013-2.650561flagellar motor protein MotB
BURPS1106A_3939015-4.234590flagellar motor protein MotA
BURPS1106A_3940118-3.537474transcriptional activator FlhC
BURPS1106A_3941017-3.299026transcriptional activator FlhD
BURPS1106A_3942114-0.986055glycoside hydrolase family protein
BURPS1106A_3943013-0.736042H-NS histone family protein
BURPS1106A_3944112-0.128629hypothetical protein
BURPS1106A_3945090.349578aquaporin Z
BURPS1106A_39463130.298578HAD family hydrolase
BURPS1106A_39473130.070088BadF/BadG/BcrA/BcrD ATPase family protein
BURPS1106A_3948415-1.859555DNA-3-methyladenine glycosidase I
BURPS1106A_3949417-1.850556hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3936PF06580463e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 46.0 bits (109), Expect = 3e-07
Identities = 21/151 (13%), Positives = 50/151 (33%), Gaps = 52/151 (34%)

Query: 467 ELDKSLIERIIDPLT--HLVRNSLDHGIETVEARRAAGKDAVGQLVLSAAHHGGNIVIEV 524
+++ ++++ + P+ LV N + HGI G+++L G + +EV
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIA--------QLPQGGKILLKGTKDNGTVTLEV 296

Query: 525 SDDGAGLNRERILAKAAKQGMQISENISDDEVWNLIFAPGFSTAEVVTDVSGRGVGMDVV 584
+ G+ + G G+ V
Sbjct: 297 ENTGSLALKNTK--------------------------------------ESTGTGLQNV 318

Query: 585 KRNIQSMGG---HVEISSQAGRGTTTRIVLP 612
+ +Q + G +++S + G +++P
Sbjct: 319 RERLQMLYGTEAQIKLSEKQG-KVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3937HTHFIS718e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 71.4 bits (175), Expect = 8e-18
Identities = 38/114 (33%), Positives = 58/114 (50%), Gaps = 2/114 (1%)

Query: 4 TILAIDDSATMRTLLSATLGEAGYDVTVASDGEVGLDVALATRFDLVLTDHHMPRKNGLE 63
TIL DD A +RT+L+ L AGYDV + S+ A DLV+TD MP +N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 LIVALRRQLGYEATPILVLTTENGDAFKDAARAAGATGWIEKPIDPDALIELVA 117
L+ +++ P+LV++ +N A GA ++ KP D LI ++
Sbjct: 65 LLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3938OMPADOMAIN401e-05 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 39.5 bits (92), Expect = 1e-05
Identities = 25/117 (21%), Positives = 51/117 (43%), Gaps = 9/117 (7%)

Query: 182 FAMSSDAVEPYMRDILREIGKTLNDV---PNRIIVQGHTDAVPYAGGEKGYSNWELSADR 238
F + ++P + L ++ L+++ ++V G+TD + G Y N LS R
Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRI----GSDAY-NQGLSERR 277

Query: 239 ANASRRELIAGGMDEAKVLRV-LGLASTQNLNKADPLDPENRRISIIVLNRKSELAL 294
A + LI+ G+ K+ +G ++ N D + I + +R+ E+ +
Sbjct: 278 AQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334


51BURPS1106A_3962BURPS1106A_3986Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3962116-3.014475Rieske family iron-sulfur cluster-binding
BURPS1106A_3963015-2.591116hypothetical protein
BURPS1106A_3964115-1.067692hypothetical protein
BURPS1106A_3965-28-0.205458LysE family translocator protein
BURPS1106A_396609-0.175185acid phosphatase AcpA
BURPS1106A_3967314-0.271812hypothetical protein
BURPS1106A_3968116-1.668948lipoprotein
BURPS1106A_3969-113-1.522788lipoprotein
BURPS1106A_3970211-0.426657csgG family protein
BURPS1106A_3971211-0.356305hypothetical protein
BURPS1106A_3972211-0.384014hypothetical protein
BURPS1106A_3973211-1.342653lipoprotein
BURPS1106A_3974211-1.3605223-oxoadipate enol-lactone hydrolase family
BURPS1106A_3975113-1.937309methyl-accepting chemotaxis protein
BURPS1106A_3976019-3.456926hypothetical protein
BURPS1106A_3977023-4.461323hypothetical protein
BURPS1106A_3978024-4.992029FAD/FMN-containing dehydrogenases
BURPS1106A_3979434-6.560474chitin-binding domain-containing protein
BURPS1106A_3980440-9.163518lipoprotein
BURPS1106A_3981441-9.107786hypothetical protein
BURPS1106A_3982541-9.325364gp30
BURPS1106A_3983437-7.013919hypothetical protein
BURPS1106A_3984636-6.688572transposase, IS4
BURPS1106A_3985219-5.204779hypothetical protein
BURPS1106A_3986217-1.191432phage integrase family site specific
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3969IGASERPTASE290.007 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 28.9 bits (64), Expect = 0.007
Identities = 16/95 (16%), Positives = 28/95 (29%)

Query: 48 QKSPEDQIDALEKALQQIRAKGNRPPPGFEAHLGMLYASVGKEQQAEQAFQAEKASFPES 107
+ + I A ++ + R S E AE + Q K
Sbjct: 996 NITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNE 1055

Query: 108 SPFMDFLLKKKAAAPQAKPSAPAQPQTQTQTQAQQ 142
+ + + A +AK + A QT Q+
Sbjct: 1056 QDATETTAQNREVAKEAKSNVKANTQTNEVAQSGS 1090


52BURPS1106A_4037BURPS1106A_4063Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_4037-111-3.271922cyclohexadienyl dehydratase
BURPS1106A_4038-113-3.705418CoA-binding protein
BURPS1106A_4039115-4.031545hypothetical protein
BURPS1106A_4040016-3.673907AMP-binding protein
BURPS1106A_4041223-4.602325F0F1 ATP synthase subunit epsilon
BURPS1106A_4042225-4.870001F0F1 ATP synthase subunit beta
BURPS1106A_4043120-5.001437F0F1 ATP synthase subunit gamma
BURPS1106A_4044121-4.667933F0F1 ATP synthase subunit alpha
BURPS1106A_4045524-4.816082F0F1 ATP synthase subunit delta
BURPS1106A_4046426-6.130678F0F1 ATP synthase subunit B
BURPS1106A_4047018-4.408674F0F1 ATP synthase subunit C
BURPS1106A_4048-115-3.507647F0F1 ATP synthase subunit A
BURPS1106A_4049018-3.330695F0F1-type ATP synthase subunit I
BURPS1106A_4050-120-3.707574lipoprotein
BURPS1106A_4052024-4.935338hypothetical protein
BURPS1106A_4051024-4.907458transporter
BURPS1106A_4053226-5.298737ParB family protein
BURPS1106A_4054126-5.181870CobQ/CobB/MinD/ParA nucleotide binding
BURPS1106A_4055124-4.34209516S rRNA methyltransferase GidB
BURPS1106A_4056225-3.437015tRNA uridine 5-carboxymethylaminomethyl
BURPS1106A_4057019-2.205126hydrophobic amino acid ABC transporter
BURPS1106A_4058-216-2.016094hydrophobic amino acid ABC transporter
BURPS1106A_4059-214-1.292034amino acid uptake ABC transporter permease
BURPS1106A_4060-213-1.857062amino acid uptake ABC transporter periplasmic
BURPS1106A_4061-211-1.729283hypothetical protein
BURPS1106A_4062-111-2.903161amino acid uptake ABC transporter permease
BURPS1106A_4063-211-3.677456amino acid uptake ABC transporter permease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_4045FLGMOTORFLIN270.034 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 26.8 bits (59), Expect = 0.034
Identities = 24/87 (27%), Positives = 45/87 (51%), Gaps = 9/87 (10%)

Query: 5 ATIARPYAEALFRVAEGGDISAWSTLVQELAQVAQLPEVLSVASSPKVSRTQ--VAELLL 62
AT + A+A+F+ GGD+S +Q++ + +P L+V ++ RT+ + ELL
Sbjct: 28 ATTTKSAADAVFQQLGGGDVSG---AMQDIDLIMDIPVKLTV----ELGRTRMTIKELLR 80

Query: 63 AALKSPLASGAQAKNFVQMLVDNHRIA 89
S +A A + +L++ + IA
Sbjct: 81 LTQGSVVALDGLAGEPLDILINGYLIA 107


53BURPS1106A_0007BURPS1106A_0015N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0007-110-0.577737general secretion pathway protein D
BURPS1106A_00081120.574303general secretory pathway protein E
BURPS1106A_0009-1150.570654general secretion pathway protein F
BURPS1106A_00100141.439279general secretion pathway protein C
BURPS1106A_00110131.864629hypothetical protein
BURPS1106A_00120133.210472general secretion pathway protein G
BURPS1106A_0013-1143.887464general secretion pathway protein H
BURPS1106A_00140133.602457general secretion pathway protein I
BURPS1106A_0015-2114.122225general secretion pathway protein J
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0007BCTERIALGSPD403e-133 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 403 bits (1038), Expect = e-133
Identities = 215/691 (31%), Positives = 324/691 (46%), Gaps = 88/691 (12%)

Query: 13 TALVVAGIVAAQAAHAQVTLNFVNADIDQVAKAIGAATGKTIIVDPRVKGQLNLVAERPV 72
T L+ A ++ AA + + +F DI + + KT+I+DP V+G + + + +
Sbjct: 13 TLLIFAALLFRPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDML 72

Query: 73 PEDQALKTLQSALRMQGFALV-QDHGVLKVVPEADAKLQGVPTYIGNAPQARGDQVVTQV 131
E+Q + S L + GFA++ ++GVLKVV DAK VP AP GD+VVT+V
Sbjct: 73 NEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDAKTAAVPVASDAAPGI-GDEVVTRV 131

Query: 132 FELRNESANNLLPVLRPLI--SPNNTITAYPANNTIVVTDYADNVRRIAQIIAGVDSAAG 189
L N +A +L P+LR L + ++ Y +N +++T A ++R+ I+ VD+A
Sbjct: 132 VPLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGD 191

Query: 190 SQVAVVPLKNANAIDIAAQLTKLLDPGAIGNTDATLKVTVQADPRTNALLLRASNAQRLA 249
V VPL A+A D+ +T+L + ++ V AD RTNA+L+ R
Sbjct: 192 RSVVTVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSR-Q 250

Query: 250 AAKKIAQQLDAPSGVPGNMHVVPLRNAEAVKLAKTLRGMLGKGGGESGSSASSNDANAFN 309
+ +QLD GN V+ L+ A+A L + L G+
Sbjct: 251 RIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGIS-------------------- 290

Query: 310 QGGSQSGSNFSTGASGTPPLPSGLSSNSSGGAGGTTGGGGLGNAGLLGGDKDKGDDNQPG 369
S + S +
Sbjct: 291 ---------------------STMQSEKQAAKPVAALDKNI------------------- 310

Query: 370 GMIQADAASNSLIITASDPVYRNLRAVIDQLDARRAQVYIEALVVELQATTSANLGIQWQ 429
+I+A +N+LI+TA+ V +L VI QLD RR QV +EA++ E+Q NLGIQW
Sbjct: 311 -IIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWA 369

Query: 430 VANNALYAGTNLVTGQTGLGNSIVNLTAGAVT--NPGGTLGSLG---SITNGLNIGWLHN 484
N +T T G I AGA G SL S NG+ G
Sbjct: 370 NKNAG-------MTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAG---- 418

Query: 485 MFGVQGLGALLQFFAGSSDANVLSTPNLVTLDNEEAKIVVGQNVPIPTGSYSNLTSGTTA 544
F LL + S+ ++L+TP++VTLDN EA VGQ VP+ TGS + +
Sbjct: 419 -FYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGS----QTTSGD 473

Query: 545 NAFNTYDRRDVGLTLHVKPQITEGGILKLQLYTEDSAVVPGTNTTSANSPGPTFTKRSIQ 604
N FNT +R+ VG+ L VKPQI EG + L++ E S+V +++++ G TF R++
Sbjct: 474 NIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSV-ADAASSTSSDLGATFNTRTVN 532

Query: 605 STVLADNGEIIVLGGLMQDNYQVSNTKVPLLGDIPWIGQLFRSEGKTRQKTNLMVFLRPV 664
+ VL +GE +V+GGL+ + + KVPLLGDIP IG LFRS K K NLM+F+RP
Sbjct: 533 NAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPT 592

Query: 665 IINDRETAQAVTSNRYDYIQGVTGAYKSDNN 695
+I DR+ + +S +Y + N
Sbjct: 593 VIRDRDEYRQASSGQYTAFNDAQSKQRGKEN 623


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0009BCTERIALGSPF382e-133 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 382 bits (982), Expect = e-133
Identities = 174/406 (42%), Positives = 266/406 (65%), Gaps = 2/406 (0%)

Query: 1 MPAFRFEAIDASGRAQKGVIEADSARNARGQLRTQGLTPLVVEPAASAQRGARSQRLALG 60
M + ++A+DA G+ +G EADSAR AR LR +GL PL V+ Q+ + S L+L
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 61 R--KLSQREQAILTRQLASLLVAGLPLDEALAVLTEQAERDYIRELMAAIRAEVLGGHSL 118
R +LS + A+LTRQLA+L+ A +PL+EAL + +Q+E+ ++ +LMAA+R++V+ GHSL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 119 ANALTQHPRDFPEIYRALVAAGEHTGKLGIVLSRLADYIEERNALKQKILLAFTYPAIVT 178
A+A+ P F +Y A+VAAGE +G L VL+RLADY E+R ++ +I A YP ++T
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 179 VIAFGIVTFLLSYVVPQVVNVFASTKQQLPVLTIVMMALSDFVRHWWWAILIGIAAVVYL 238
V+A +V+ LLS VVP+VV F KQ LP+ T V+M +SD VR + +L+ + A
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 239 VKATLSRDGPRLAFDRWLLTAPLAGKLVRGYNTVRFASTLGILTAAGVPILRALQAAGET 298
+ L ++ R++F R LL PL G++ RG NT R+A TL IL A+ VP+L+A++ +G+
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 299 LSNRAMRGNIDDAIVRVREGSALSRALNNVKTFPPVLVHLIRSGEATGDVTTMLDRAAEG 358
+SN R + A VREG +L +AL FPP++ H+I SGE +G++ +ML+RAA+
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 359 ESRELERRTMFLTSLLEPLLILAMGGIVLVIVLAVMLPIIELNNMV 404
+ RE + L EPLL+++M +VL IVLA++ PI++LN ++
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLM 406


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0012BCTERIALGSPG1886e-65 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 188 bits (480), Expect = 6e-65
Identities = 67/140 (47%), Positives = 94/140 (67%), Gaps = 3/140 (2%)

Query: 10 QAARRQRGFTLIEIMVVVAILGILAALIVPKIMSRPDEARRIAAKQDIGTIMQALKLYRL 69
+A +QRGFTL+EIMVV+ I+G+LA+L+VP +M ++A + A DI + AL +Y+L
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKL 61

Query: 70 DNGRYPTQDQGLNALIQKPTTDPIPNNWKDGGYLERLPNDPWGNSYKYLNPGVHGEIDVF 129
DN YPT +QGL +L++ PT P+ N+ GY++RLP DPWGN Y +NPG HG D+
Sbjct: 62 DNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLL 121

Query: 130 SYGADGKEGGESNDSDIGSW 149
S G DG+ G E DI +W
Sbjct: 122 SAGPDGEMGTE---DDITNW 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0013BCTERIALGSPH521e-10 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 51.9 bits (124), Expect = 1e-10
Identities = 20/101 (19%), Positives = 33/101 (32%), Gaps = 15/101 (14%)

Query: 51 RARGFTLLEMLVVLVIAGILVSVASLTLRRNPRTDLREEAQRIALLFETAGDEAQVRARP 110
R RGFTLLEM+++L++ G+ + L + + R +
Sbjct: 2 RQRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQRGLQTGQF 61

Query: 111 IAWRATEHGFRF---------------DIRTGDGWRPLRDD 136
++F D +G W PLR
Sbjct: 62 FGVSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAG 102


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0014BCTERIALGSPG300.001 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 30.2 bits (68), Expect = 0.001
Identities = 10/26 (38%), Positives = 18/26 (69%)

Query: 10 RSPARSRGFTMIEVLVALAIIAVALA 35
R+ + RGFT++E++V + II V +
Sbjct: 2 RATDKQRGFTLLEIMVVIVIIGVLAS 27


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0015BCTERIALGSPG333e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 33.3 bits (76), Expect = 3e-04
Identities = 17/72 (23%), Positives = 34/72 (47%), Gaps = 3/72 (4%)

Query: 33 RGFTLIEMMIAITILAVIA-ILSWRGLDQIIRGREKVAAAMEDERVFAQMFDQMRIDARR 91
RGFTL+E+M+ I I+ V+A ++ + + ++ A+ D D ++D
Sbjct: 8 RGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK--AVSDIVALENALDMYKLDNHH 65

Query: 92 AATDDEAGQPAV 103
T ++ + V
Sbjct: 66 YPTTNQGLESLV 77


54BURPS1106A_0029BURPS1106A_0034N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0029110-0.272872flagellar motor switch protein FliM
BURPS1106A_00300121.673701flagellar motor switch protein FliN
BURPS1106A_00310112.438570flagellar biosynthesis protein FliO
BURPS1106A_0032-1122.165550flagellar biosynthesis protein FliP
BURPS1106A_0033-391.961611flagellar biosynthesis protein FliQ
BURPS1106A_0034-381.354178flagellar biosynthetic protein FliR
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0029FLGMOTORFLIM2762e-93 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 276 bits (706), Expect = 2e-93
Identities = 82/324 (25%), Positives = 159/324 (49%), Gaps = 10/324 (3%)

Query: 5 EFMSQEEVDALLKGVTGEDDSADEPAEASG---IRPYNIATQERIVRGRMPGLEIINDRF 61
E +SQ+E+D LL ++ D S ++ S I Y+ ++ + +M L ++++ F
Sbjct: 3 EVLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETF 62

Query: 62 ARLLRIGIFNFMRRTAEISVSQVKVQKYSEFTRNLPIPTNLNLVHVKPLRGTSLFVFDPN 121
ARL + +R + V+ V Y EF R++P P+ L ++ + PL+G ++ DP+
Sbjct: 63 ARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPS 122

Query: 122 LVFFVVDNLFGGDGRFHTRVEGRDFTATEQRIIGKLLNLVFEHYASAWKSVRPLQFEFVR 181
+ F ++D LFGG G+ RD T E ++ ++ + + +W V L+ +
Sbjct: 123 ITFSIIDRLFGGTGQAAKVQ--RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQ 180

Query: 182 SEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEAL-- 239
E + QFA + P+E+V++ + G G ++ C+PY IEPI LSS ++
Sbjct: 181 IETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRR 240

Query: 240 EVDRRWVRVLSQQVQSAEVELVADLAEVPTTFEKILNLRTGDVLPLD---ITDSITAKVD 296
+++ VL ++ + ++++VA++ + + IL LR GD++ L + D +
Sbjct: 241 SSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIG 300

Query: 297 GVPVMECGYGIFNGQYALRVQRMI 320
C G+ + A ++ I
Sbjct: 301 NRKKFLCQPGVVGKKIAAQILERI 324


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0030FLGMOTORFLIN1343e-43 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 134 bits (338), Expect = 3e-43
Identities = 78/126 (61%), Positives = 97/126 (76%), Gaps = 3/126 (2%)

Query: 41 AMDD-WAAALAEQNQQPIETGATGAGVFRPLSKATASSTHNDIDLILDIPVKMTVELGRT 99
A+DD WA AL EQ ++ A VF+ L S DIDLI+DIPVK+TVELGRT
Sbjct: 14 ALDDLWADALNEQKATTTKSAADA--VFQQLGGGDVSGAMQDIDLIMDIPVKLTVELGRT 71

Query: 100 KIAIRNLLQLAQGSVVELDGLAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPSER 159
++ I+ LL+L QGSVV LDGLAGEP+D+L+NG LIAQGEVVVV DK+G+R+TDIITPSER
Sbjct: 72 RMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSER 131

Query: 160 IRKLNR 165
+R+L+R
Sbjct: 132 MRRLSR 137


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0032FLGBIOSNFLIP297e-104 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 297 bits (761), Expect = e-104
Identities = 154/242 (63%), Positives = 192/242 (79%), Gaps = 1/242 (0%)

Query: 34 RWLPAILIGLAPALACAQAAGLPAFNSAPGPNGGTTYSLSVQTMLLLTMLSFLPAMLLMM 93
R L + L + A LP S P P GG ++SL VQT++ +T L+F+PA+LLMM
Sbjct: 3 RLLSVAPVLLW-LITPLAFAQLPGITSQPLPGGGQSWSLPVQTLVFITSLTFIPAILLMM 61

Query: 94 TSFTRIIIVLSLLRQAIGTASTPPNQVLVGLALFLTLFVMSPVLDRAYNDAYKPFSEGTL 153
TSFTRIIIV LLR A+GT S PPNQVL+GLALFLT F+MSPV+D+ Y DAY+PFSE +
Sbjct: 62 TSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMSPVIDKIYVDAYQPFSEEKI 121

Query: 154 QMDQAVQRGTAPFKAFMLKQTRETDLALFAKISKAAPMQGPEDVPLSLLVPAFVTSELKT 213
M +A+++G P + FML+QTRE DL LFA+++ P+QGPE VP+ +L+PA+VTSELKT
Sbjct: 122 SMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQGPEAVPMRILLPAYVTSELKT 181

Query: 214 GFQIGFTIFIPFLIIDMVVASVLMSMGMMMVSPATVSLPFKLMLFVLVDGWQLLIGSLAQ 273
FQIGFTIFIPFLIID+V+ASVLM++GMMMV PAT++LPFKLMLFVLVDGWQLL+GSLAQ
Sbjct: 182 AFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQ 241

Query: 274 SF 275
SF
Sbjct: 242 SF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0033TYPE3IMQPROT694e-19 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 68.6 bits (168), Expect = 4e-19
Identities = 26/85 (30%), Positives = 46/85 (54%)

Query: 4 ENVMTLAHQAMYIGLLLAAPLLLVALAVGLVVSLFQAATQINEATLSFIPKLLAVAATMV 63
++++ ++A+Y+ L+L+ +VA +GL+V LFQ TQ+ E TL F KLL V +
Sbjct: 2 DDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLF 61

Query: 64 IAGPWMLSTMIDYLRETLLRVATLG 88
+ W ++ Y R+ + G
Sbjct: 62 LLSGWYGEVLLSYGRQVIFLALAKG 86


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0034TYPE3IMRPROT1615e-51 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 161 bits (410), Expect = 5e-51
Identities = 117/250 (46%), Positives = 159/250 (63%), Gaps = 1/250 (0%)

Query: 1 MFSVTYAQLNGWLTAFLWPFVRMLALVAIAPVTGHRSTPVRVKIGLAGFMALVVAPTLPP 60
M VT Q WL + WP +R+LAL++ AP+ RS P RVK+GLA + +AP+LP
Sbjct: 1 MLQVTSEQWLSWLNLYFWPLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPA 60

Query: 61 MPVATVFSAQGVWIIVNQFLIGAALGFTMQIVFAAIEAAGDIIGLSMGLGFATFFDPHSS 120
V VFS +W+ V Q LIG ALGFTMQ FAA+ AG+IIGL MGL FATF DP S
Sbjct: 61 NDVP-VFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASH 119

Query: 121 GATPVMGRFLNAVAILAFLAFDGHLQVFAALVDSFRLVPVSADLLRAAGWQTLVAFGAAI 180
PV+ R ++ +A+L FL F+GHL + + LVD+F +P+ + L + + L G+ I
Sbjct: 120 LNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLI 179

Query: 181 FEMGLLLALPVVAALLIANLALGILNRAAPQIGIFQVGFPVTMLVGLLLVQLMAPNLIPF 240
F GL+LALP++ LL NLALG+LNR APQ+ IF +GFP+T+ VG+ L+ + P + PF
Sbjct: 180 FLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPF 239

Query: 241 VGRLFDTGVD 250
LF +
Sbjct: 240 CEHLFSEIFN 249


55BURPS1106A_0175BURPS1106A_0184N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0175-4101.430480HpcH/HpaI aldolase/citrate lyase family protein
BURPS1106A_0176-3100.329888hypothetical protein
BURPS1106A_0177-111-0.386657rod shape-determining protein RodA
BURPS1106A_0178-1130.543651penicillin-binding protein
BURPS1106A_0179012-0.290778rod shape-determining protein MreD
BURPS1106A_0180012-0.586216rod shape-determining protein MreC
BURPS1106A_0181012-1.918107rod shape-determining protein MreB
BURPS1106A_0182-211-1.748741aspartyl/glutamyl-tRNA amidotransferase subunit
BURPS1106A_0183-110-1.251246aspartyl/glutamyl-tRNA amidotransferase subunit
BURPS1106A_0184-211-0.947636aspartyl/glutamyl-tRNA amidotransferase subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0175PHPHTRNFRASE443e-07 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 44.0 bits (104), Expect = 3e-07
Identities = 36/178 (20%), Positives = 60/178 (33%), Gaps = 34/178 (19%)

Query: 87 RALDAGARTLMFPGVETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVQT 146
RA G +MFP + T +E LR I++ + + V
Sbjct: 380 RASTYGNLKVMFPMIATLEE------------------LRQAKAIMQEEKDKLLSEGVDV 421

Query: 147 ANAQIATIVQIESARGVDEAERIAATPGVDCVFVGPADL----------SASLGHLGDTK 196
++ I + +E A A VD +G DL + + +L
Sbjct: 422 SD-SIEVGIMVEIPSTAVAANLFA--KEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPY 478

Query: 197 HPDVAAALEHVLAAGRRAGVPVGI---FAADTAGARQSLEAGFRVVALSADVVWLLRA 251
HP + ++ V+ A G VG+ A D L G ++SA + R+
Sbjct: 479 HPAILRLVDMVIKAAHSEGKWVGMCGEMAGDEVAIPLLLGLGLDEFSMSATSILPARS 536


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0178cloacin340.003 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 33.9 bits (77), Expect = 0.003
Identities = 25/82 (30%), Positives = 31/82 (37%), Gaps = 11/82 (13%)

Query: 681 SGADGASGASGASGAS--------GAGGEPTEHANAGGNPAG-GGIAGGAAGTANNGSGA 731
+G GAS SG S G+G +G G G +GG +GT N S
Sbjct: 25 TGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAV 84

Query: 732 AAPG--GMPGANGAATGAPPAS 751
AAP G P + G S
Sbjct: 85 AAPVAFGFPALSTPGAGGLAVS 106



Score = 33.5 bits (76), Expect = 0.003
Identities = 20/71 (28%), Positives = 27/71 (38%)

Query: 685 GASGASGASGASGAGGEPTEHANAGGNPAGGGIAGGAAGTANNGSGAAAPGGMPGANGAA 744
G +G GAS G +E+ GG G GG +G N G + GG +
Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82

Query: 745 TGAPPASRRLP 755
A P + P
Sbjct: 83 AVAAPVAFGFP 93


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0180GPOSANCHOR280.046 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 28.5 bits (63), Expect = 0.046
Identities = 16/64 (25%), Positives = 22/64 (34%), Gaps = 3/64 (4%)

Query: 293 KAAKGKKATKGADKSAKAADKGADKDKGAKPAAAPPVPARSRPAGPAQPAAPLKPATAPS 352
K + +KA A A+A A K+K AK A + + P A P
Sbjct: 424 KLTEKEKAELQAKLEAEAK---ALKEKLAKQAEELAKLRAGKASDSQTPDAKPGNKAVPG 480

Query: 353 PGAP 356
G
Sbjct: 481 KGQA 484


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0181SHAPEPROTEIN5040.0 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 504 bits (1300), Expect = 0.0
Identities = 247/348 (70%), Positives = 294/348 (84%), Gaps = 2/348 (0%)

Query: 1 MFGFLRSYFSNDLAIDLGTANTLIYMRGKGIVLDEPSVVSIRQEGGPNGKKTIQAVGKEA 60
M R FSNDL+IDLGTANTLIY++G+GIVL+EPSVV+IRQ+ K++ AVG +A
Sbjct: 1 MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRA-GSPKSVAAVGHDA 59

Query: 61 KQMLGKVPGNIEAIRPMKDGVIADFTVTEQMIKQFIKTAHESRMFSPSPRIIICVPCGST 120
KQMLG+ PGNI AIRPMKDGVIADF VTE+M++ FIK H + PSPR+++CVP G+T
Sbjct: 60 KQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGAT 119

Query: 121 QVERRAIKEAAHGAGASQVYLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVGVISLG 180
QVERRAI+E+A GAGA +V+LIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEV VISL
Sbjct: 120 QVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLN 179

Query: 181 GIVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEAIKKEIGSAFPGSEVKEMEVKGR 240
G+VY SVR+GGD+FDEAI+NY+RRNYG LIGE TAE IK EIGSA+PG EV+E+EV+GR
Sbjct: 180 GVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGR 239

Query: 241 NLSEGIPRSFTISSNEILEALTDPLNQIVSSVKIALEQTPPELGADIAERGMMLTGGGAL 300
NL+EG+PR FT++SNEILEAL +PL IVS+V +ALEQ PPEL +DI+ERGM+LTGGGAL
Sbjct: 240 NLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGAL 299

Query: 301 LRDLDRLLAEETGLPVLVAEDPLTCVVRGSGMALERMDKL-GSIFSYE 347
LR+LDRLL EETG+PV+VAEDPLTCV RG G ALE +D G +FS E
Sbjct: 300 LRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSEE 347


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0184TYPE4SSCAGA310.013 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 30.8 bits (69), Expect = 0.013
Identities = 29/89 (32%), Positives = 43/89 (48%), Gaps = 5/89 (5%)

Query: 395 SNKIAKEIFVTIWDEKAADEGAADRIIEAKGLK-QISDTGALEAIIDEVLAANAKSVEEF 453
+N EIF I E D A KG+K ++SD LE + ++ L KS +EF
Sbjct: 648 ANSQKDEIFALINKEANRDARAIAYAQNLKGIKRELSDK--LENV-NKNLKDFDKSFDEF 704

Query: 454 RAGKDKAFNALVGQAMKATKGKANPQQVN 482
+ GK+K F+ + +KA KG +N
Sbjct: 705 KNGKNKDFSK-AEETLKALKGSVKDLGIN 732


56BURPS1106A_0193BURPS1106A_0200N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_01930100.245647nucleoid occlusion protein
BURPS1106A_0194011-0.042375HAD-superfamily hydrolase
BURPS1106A_0195113-1.585882acetylglutamate kinase
BURPS1106A_0196011-0.944622hypothetical protein
BURPS1106A_0197-112-0.722698hypothetical protein
BURPS1106A_0198010-2.031934sensor histidine kinase
BURPS1106A_0199312-3.084121Fis family DNA-binding response regulator
BURPS1106A_0200213-4.106789ATP-dependent protease ATP-binding subunit HslU
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0193HTHTETR581e-12 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 58.1 bits (140), Expect = 1e-12
Identities = 31/183 (16%), Positives = 62/183 (33%), Gaps = 15/183 (8%)

Query: 24 ASRTRPKPGERRVHILQTLASMLEAPKSEKITTAALAARLDVSEAALYRHFSSKAQMFEG 83
A +T+ + E R HIL + + +A V+ A+Y HF K+ +F
Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 84 LIEFIEETFFGLVNQIAANEPNGVLQA-RSIALMLLNFSAKNPGMTRVLTGEALVGEHER 142
+ E E L + A P L R I + +L + ++ + H+
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLME----IIFHKC 117

Query: 143 LAERVNQMLERVEASIKQCLR---VALLEAQAHAAGGAPPPVPLPDDYDPALRASLVISY 199
++++ + ++ L+ A P + A ++ Y
Sbjct: 118 EFVGEMAVVQQAQRNLCLESYDRIEQTLKH-CIEAKMLPADL------MTRRAAIIMRGY 170

Query: 200 VLG 202
+ G
Sbjct: 171 ISG 173


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0195CARBMTKINASE445e-07 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 43.7 bits (103), Expect = 5e-07
Identities = 27/99 (27%), Positives = 48/99 (48%), Gaps = 6/99 (6%)

Query: 180 IPVISPIGFGEDGLSYNINADLVAGKLATVLNAEKLVMMTNIPGVMDKEG----NLLTDL 235
+PVI G G+ I+ DL KLA +NA+ +++T++ G G L ++
Sbjct: 197 VPVILEDG-EIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNGAALYYGTEKEQWLREV 255

Query: 236 SAREIDALFEDGT-ISGGMLPKISSALDAAKSGVKSVHI 273
E+ +E+G +G M PK+ +A+ + G + I
Sbjct: 256 KVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWGGERAII 294



Score = 36.7 bits (85), Expect = 8e-05
Identities = 21/60 (35%), Positives = 27/60 (45%), Gaps = 10/60 (16%)

Query: 31 GKTVVIKYGGNAMTEERLKQGF----------ARDVILLKLVGINPVIVHGGGPQIDQAL 80
GK VVI GGNA+ + K + AR + + G VI HG GPQ+ L
Sbjct: 2 GKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSLL 61


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0199HTHFIS889e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 87.6 bits (217), Expect = 9e-23
Identities = 30/127 (23%), Positives = 60/127 (47%)

Query: 1 MSDKNFLVIDDNEVFAGTLARGLERRGYAVRQAHNKDEALKLAGAEKFEFITVDLHLGND 60
M+ LV DD+ L + L R GY VR N + A + + D+ + ++
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 61 SGLSLIAPLCDLQPDARILVLTGYASIATAVQAVKDGADNYLAKPANVESILAALQTNAS 120
+ L+ + +PD +LV++ + TA++A + GA +YL KP ++ ++ + +
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 121 EVQAEEA 127
E + +
Sbjct: 121 EPKRRPS 127



Score = 45.2 bits (107), Expect = 4e-08
Identities = 16/101 (15%), Positives = 32/101 (31%), Gaps = 3/101 (2%)

Query: 75 DARILVLTGYASIATAVQAVKDGADNYLAKPANVESILAALQTNASEVQAEEALENPVVL 134
I+ + I + L+ VE + + + L
Sbjct: 375 TREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFGDALPPSGL---YDR 431

Query: 135 SVDRLEWEHIQRVLAENNNNISATARALNMHRRTLQRKLAK 175
+ +E+ I L N A L ++R TL++K+ +
Sbjct: 432 VLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRE 472


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0200HTHFIS310.016 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 30.6 bits (69), Expect = 0.016
Identities = 13/68 (19%), Positives = 29/68 (42%), Gaps = 15/68 (22%)

Query: 17 IIGQAKAKKAVAVALRNRWRRQQVAEPLRQEITPKNILMIGPTGVGKTEIAR---RLAKL 73
++G++ A + + ++ + T +++ G +G GK +AR K
Sbjct: 139 LVGRSAAMQEI------YRVLARLMQ------TDLTLMITGESGTGKELVARALHDYGKR 186

Query: 74 ADAPFIKI 81
+ PF+ I
Sbjct: 187 RNGPFVAI 194


57BURPS1106A_0229BURPS1106A_0239N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_02291123.255666flagellar hook-length control protein FliK
BURPS1106A_02302141.707573flagellar export protein FliJ
BURPS1106A_02313121.340081flagellar protein export ATPase FliI
BURPS1106A_02321120.313184flagellar assembly protein H
BURPS1106A_02331112.406141flagellar motor switch protein G
BURPS1106A_02340103.790535flagellar MS-ring protein
BURPS1106A_02351104.651683flagellar hook-basal body complex protein FliE
BURPS1106A_0236194.860210flagellar protein FliS
BURPS1106A_0237-283.974993hypothetical protein
BURPS1106A_0238-193.308691hypothetical protein
BURPS1106A_0239-1122.159717flagellar biosynthesis protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0229FLGHOOKFLIK733e-16 Flagellar hook-length control protein signature.
		>FLGHOOKFLIK#Flagellar hook-length control protein signature.

Length = 375

Score = 73.3 bits (179), Expect = 3e-16
Identities = 79/257 (30%), Positives = 109/257 (42%), Gaps = 8/257 (3%)

Query: 205 NGDASAPLAANRAAFDKLLAGAKAPAAQAAPTDASGANPATALANAAANAAQPDASG--A 262
N D +A L+A A K A + T L + AQPD +
Sbjct: 124 NEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKPTLFTKLTSEQLTTAQPDDAPGTP 183

Query: 263 LAALQDAADSARATLAASSAPAALQQAA-PAALAANASAAAASAAPSLAPPVGTPDWTDA 321
L A++ S P+ + AA P AAP L+ P+G+ +W +
Sbjct: 184 AQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPHQTQPLPTVAAPVLSAPLGSHEWQQS 243

Query: 322 LSQKVVFLSNAHQQSAELTLNPPDLGPLQVVLRVADNHAHALFVSQHAQVRDAVEAALPK 381
LSQ + + QQSAEL L+P DLG +Q+ L+V DN A VS H VR A+EAALP
Sbjct: 244 LSQHISLFTRQGQQSAELRLHPQDLGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPV 303

Query: 382 LREAMEAGGLGLGSASVSDGGFASAQQQQTPQRQSSDGSATRRAFGASTADAALDELAAA 441
LR + G+ LG +++S F+ QQ + Q+QS +A D L
Sbjct: 304 LRTQLAESGIQLGQSNISGESFSGQQQAASQQQQSQR-TANHEPLAGEDDDT----LPVP 358

Query: 442 SSGGAARRAVGMVDTFA 458
S VD FA
Sbjct: 359 VSLQGRVTGNSGVDIFA 375


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0230FLGFLIJ602e-14 Flagellar FliJ protein signature.
		>FLGFLIJ#Flagellar FliJ protein signature.

Length = 147

Score = 59.8 bits (144), Expect = 2e-14
Identities = 43/140 (30%), Positives = 74/140 (52%)

Query: 1 MAQSFPLQLLLERAQDDLDTAAKQLGRAQRERTDAQAQLDALMRYRDEYRVRFAESAQSG 60
MA+ L L + A+ +++ AA+ LG +R A+ QL L+ Y++EYR +G
Sbjct: 1 MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLIDYQNEYRNNLNSDMSAG 60

Query: 61 MPAGNWRNFQAFLDTLDAAIEQQRRVLAAAQTRIDAARPEWQAKKRTLGSYEILQARGAR 120
+ + W N+Q F+ TL+ AI Q R+ L ++D A W+ KK+ L +++ LQ R +
Sbjct: 61 ITSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWREKKQRLQAWQTLQERQST 120

Query: 121 QDAQRAAKREQRDADEHAAK 140
+ +Q+ DE A +
Sbjct: 121 AALLAENRLDQKKMDEFAQR 140


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0232FLGFLIH1083e-31 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 108 bits (271), Expect = 3e-31
Identities = 64/184 (34%), Positives = 106/184 (57%), Gaps = 4/184 (2%)

Query: 37 AAAALAAELQRVRDAAHAEGLAAGHVEGQALGYQAGYEQGRAKGFDEGRAEAHTHAAQLA 96
A +L +L +++ AH +G AG EG+ G++ GY++G A+G ++G AEA + A +
Sbjct: 36 AEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIH 95

Query: 97 A----LAASFRDALAGVERDLADDIATLALEIAQQVVRQHVQHDPAALIAAAREVLAAEP 152
A L + F+ L ++ +A + +ALE A+QV+ Q D +ALI +++L EP
Sbjct: 96 ARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEP 155

Query: 153 ALAGAPHLIVNPADLPVVEAYLKDELDTLGWSVRTDTSIERGGCRAHASTGEIDATLTTR 212
+G P L V+P DL V+ L L GW +R D ++ GGC+ A G++DA++ TR
Sbjct: 156 LFSGKPQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATR 215

Query: 213 WERV 216
W+ +
Sbjct: 216 WQEL 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0233FLGMOTORFLIG298e-102 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 298 bits (765), Expect = e-102
Identities = 114/324 (35%), Positives = 191/324 (58%)

Query: 5 GLNKSALLLMSIGEEEAAQVFKFLAPREVQKIGAAMAALKNVTREQVEDVLNDFVQEAEK 64
G K+A+LL+SIG E +++VFK+L+ E++ + +A L+ +T E ++VL +F +
Sbjct: 17 GKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEFKELMMA 76

Query: 65 HTALSLDSSEYIRTVLTKALGEDKAGVLIDRILQGSDTSGIEGLKWMDSAAVAELIKNEH 124
+ +Y R +L K+LG KA +I+ + + E ++ D A + I+ EH
Sbjct: 77 QEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFVRRADPANILNFIQQEH 136

Query: 125 PQIIATILVHLDRDQASEIASCFTERLRNDVLLRIATLDGIQPTALRELDDVLTGLLSGS 184
PQ IA IL +LD +AS I S ++ +V RIA +D P +RE++ VL L+
Sbjct: 137 PQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPEVVREVERVLEKKLASL 196

Query: 185 DNLKRAPMGGIRTAAEILNFMTSVHEEAVIENVKQYDPDLAQKIIDQMFVFENLLDLEDR 244
+ GG+ EI+N E+ +IE++++ DP+LA++I +MFVFE+++ L+DR
Sbjct: 197 SSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEIKKKMFVFEDIVLLDDR 256

Query: 245 AIQLLLKEVESEALIIALKGAPPALRQKFLSNMSQRAAELLAEDLDARGPVRVSEVETQQ 304
+IQ +L+E++ + L ALK +++K NMS+RAA +L ED++ GP R +VE Q
Sbjct: 257 SIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKEDMEFLGPTRRKDVEESQ 316

Query: 305 RKILQVVRNLAESGQIVIGGKAED 328
+KI+ ++R L E G+IVI E+
Sbjct: 317 QKIVSLIRKLEEQGEIVISRGGEE 340


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0234FLGMRINGFLIF468e-162 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 468 bits (1206), Expect = e-162
Identities = 254/562 (45%), Positives = 360/562 (64%), Gaps = 37/562 (6%)

Query: 53 LSRMKTNPRLPFLIGAALAIAAIVALVLWSRAPDYRVLYSNLSDRDGGAIIAALQQANVP 112
L+R++ NPR+P ++ + A+A +VA+VLW++ PDYR L+SNLSD+DGGAI+A L Q N+P
Sbjct: 16 LNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIP 75

Query: 113 YKFADAGGAILVPANQVHETRLKLAAMGLPKGGSVGFELMDNQKFGISQFAEQVNYQRAL 172
Y+FA+ GAI VPA++VHE RL+LA GLPKGG+VGFEL+D +KFGISQF+EQVNYQRAL
Sbjct: 76 YRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRAL 135

Query: 173 EGELQRTVESINAVRAARVHLAIPKPSVFVRDREAPSASVLVDLYPGRVLDEGQVLAVTR 232
EGEL RT+E++ V++ARVHLA+PKPS+FVR++++PSASV V L PGR LDEGQ+ AV
Sbjct: 136 EGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVH 195

Query: 233 MVSSSVPDMPAKNVTIVDQDGNLLTQT-ASATGLDASQLKYVQQIERNTQKRIDAILAPI 291
+VSS+V +P NVT+VDQ G+LLTQ+ S L+ +QLK+ +E Q+RI+AIL+PI
Sbjct: 196 LVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDVESRIQRRIEAILSPI 255

Query: 292 FGAGNARSQVSADVDFSKIEQTSESYGPNGTPQQSAIRSQQTSSSTELAQSGASGVPGAL 351
G GN +QV+A +DF+ EQT E Y PNG ++ +RS+Q + S ++ GVPGAL
Sbjct: 256 VGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGAL 315

Query: 352 SNTPPQPASAPIVA-------------SNGQPAGPAATPVSDRKDSTTNYELDKTVRHVE 398
SN P P API ++ +A P S +++ T+NYE+D+T+RH +
Sbjct: 316 SNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHTK 375

Query: 399 QSMGTIKRLSVAVVVNYQPSTDAKGRVTMQPLAADKLAQVQQLVKDAMGYDEKRGDSVNV 458
++G I+RLSVAVVVNY+ D K PL AD++ Q++ L ++AMG+ +KRGD++NV
Sbjct: 376 MNVGDIERLSVAVVVNYKTLADGKP----LPLTADQMKQIEDLTREAMGFSDKRGDTLNV 431

Query: 459 VNSAFSAAADPFANLPWWRQPDMIELGKDIAKWLGVAAAAAALYFMFVRPALRR---AFP 515
VNS FSA + LP+W+Q I+ +WL V A L+ VRP L R
Sbjct: 432 VNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVRPQLTRRVEEAK 491

Query: 516 PPAEPAAAAVPALDGPDDMLALDGLPSPDKKQLAEEDEEHPALLAFENERNRYERNLDYA 575
E A + + L+ D + N+R E
Sbjct: 492 AAQEQAQVRQETEEAVEVRLSKDEQLQQRR----------------ANQRLGAEVMSQRI 535

Query: 576 RTIARQDPKIVATVVKNWVSDE 597
R ++ DP++VA V++ W+S++
Sbjct: 536 REMSDNDPRVVALVIRQWMSND 557


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0235FLGHOOKFLIE619e-16 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 61.2 bits (148), Expect = 9e-16
Identities = 47/111 (42%), Positives = 62/111 (55%), Gaps = 8/111 (7%)

Query: 3 APVNGIASALQQMQAMAAQAAGGASPATSLAGSGAASAGSFASAMKASLDKISGDQQKAL 62
+ + GI + Q+QA A A S SFA + A+LD+IS Q A
Sbjct: 1 SAIQGIEGVISQLQATAMSARAQES--------LPQPTISFAGQLHAALDRISDTQTAAR 52

Query: 63 GEAHAFEIGAQNVSLNDVMVDMQKANIGFQFGLQVRNKLVSAYNEIMQMSV 113
+A F +G V+LNDVM DMQKA++ Q G+QVRNKLV+AY E+M M V
Sbjct: 53 TQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0239TYPE3IMSPROT624e-15 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 62.5 bits (152), Expect = 4e-15
Identities = 17/81 (20%), Positives = 32/81 (39%), Gaps = 1/81 (1%)

Query: 10 AVLAYDAKGGDTAPRVVAKGYGLVAERIIERARDAGLYVHTAPEMV-SLLMQVDLDARIP 68
A+ +G P V K + + + A + G+ + + +L +D IP
Sbjct: 268 AIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGVPILQRIPLARALYWDALVDHYIP 327

Query: 69 PQLYQAVAELLAWLYALERDA 89
+ +A AE+L WL +
Sbjct: 328 AEQIEATAEVLRWLERQNIEK 348


58BURPS1106A_0279BURPS1106A_0289N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0279418-1.860781flagellar basal body rod protein FlgC
BURPS1106A_0280420-0.822271flagellar basal body rod modification protein
BURPS1106A_0281020-0.870910flagellar hook protein FlgE
BURPS1106A_0282-118-0.123650flagellar basal body rod protein FlgF
BURPS1106A_0283117-0.166037flagellar basal body rod protein FlgG
BURPS1106A_02842170.088970flagellar basal body L-ring protein
BURPS1106A_02853150.280636flagellar basal body P-ring protein
BURPS1106A_0286112-0.045075flagellar rod assembly protein/muramidase FlgJ
BURPS1106A_02872110.372267hypothetical protein
BURPS1106A_02881110.742685flagellar hook-associated protein FlgK
BURPS1106A_02890131.543707flagellar hook-associated protein FlgL
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0279FLGHOOKAP1270.029 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 26.8 bits (59), Expect = 0.029
Identities = 10/38 (26%), Positives = 17/38 (44%)

Query: 102 NVDPVQEMVNMISASRSYQANVETLNTAKQLMLKTLTI 139
V+ +E N+ + Y AN + L TA + + I
Sbjct: 508 GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0281FLGHOOKAP1340.001 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 34.2 bits (78), Expect = 0.001
Identities = 17/58 (29%), Positives = 24/58 (41%)

Query: 356 ISAPGSTNHGTLQGSALENSNVDLTSQLVKLITAQRNYQANAQTIKTQQTVDQTLINL 413
SA L S V+L + L Q+ Y ANAQ ++T + LIN+
Sbjct: 488 SSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 30.3 bits (68), Expect = 0.017
Identities = 11/31 (35%), Positives = 17/31 (54%)

Query: 6 GLSGLAGASSDLDVIGNNIANANTVGFKGST 36
+SGL A + L+ NNI++ N G+ T
Sbjct: 7 AMSGLNAAQAALNTASNNISSYNVAGYTRQT 37


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0282FLGHOOKAP1290.018 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 29.2 bits (65), Expect = 0.018
Identities = 9/34 (26%), Positives = 18/34 (52%)

Query: 4 LIYTAMTGATQSLEQQSVVANNLANASTTGFRAQ 37
LI AM+G + + +NN+++ + G+ Q
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQ 36


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0283FLGHOOKAP1421e-06 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 42.3 bits (99), Expect = 1e-06
Identities = 10/48 (20%), Positives = 23/48 (47%)

Query: 213 TLKQGYVESSNVNVVQELVNMIQTQRAYEINSKAVTTSDQMLQTVTQM 260
L S VN+ +E N+ + Q+ Y N++ + T++ + + +
Sbjct: 498 QLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545



Score = 40.3 bits (94), Expect = 5e-06
Identities = 19/80 (23%), Positives = 34/80 (42%), Gaps = 14/80 (17%)

Query: 4 SLYIAATGMNAQQAQMDVISNNLANVSTNGFKGSRAVFEDLLYQTVRQPGANSTQQTELP 63
+ A +G+NA QA ++ SNN+++ + G+ RQ + + L
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYT--------------RQTTIMAQANSTLG 48

Query: 64 SGLQLGTGVQQVATERLYTQ 83
+G +G GV +R Y
Sbjct: 49 AGGWVGNGVYVSGVQREYDA 68


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0284FLGLRINGFLGH2063e-69 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 206 bits (526), Expect = 3e-69
Identities = 128/222 (57%), Positives = 156/222 (70%), Gaps = 7/222 (3%)

Query: 25 AALAAAALALAGCAQIPREPITQQPMSAMPPMPPAMQAPGSIY---NPGYAG-RPLFEDQ 80
A + L+L GCA IP P+ Q SA P P A GSI+ P G +PLFED+
Sbjct: 10 AISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPTPVANGSIFQSAQPINYGYQPLFEDR 69

Query: 81 RPRNVGDILTIVIAENINATKSSGANTNRQGNTSFDVPTAG-FLGGLF--NKANLSAQGA 137
RPRN+GD LTIV+ EN++A+KSS AN +R G T+F T +L GLF +A++ A G
Sbjct: 70 RPRNIGDTLTIVLQENVSASKSSSANASRDGKTNFGFDTVPRYLQGLFGNARADVEASGG 129

Query: 138 NKFAATGGASAANTFNGTITVTVTNVLPNGNLVVSGEKQMLINQGNEFVRFSGIVNPNTI 197
N F GGA+A+NTF+GT+TVTV VL NGNL V GEKQ+ INQG EF+RFSG+VNP TI
Sbjct: 130 NTFNGKGGANASNTFSGTLTVTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTI 189

Query: 198 SGQNSVYSTQVADARIEYSAKGYINEAETMGWLQRFFLNIAP 239
SG N+V STQVADARIEY GYINEA+ MGWLQRFFLN++P
Sbjct: 190 SGSNTVPSTQVADARIEYVGNGYINEAQNMGWLQRFFLNLSP 231


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0285FLGPRINGFLGI371e-129 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 371 bits (953), Expect = e-129
Identities = 164/392 (41%), Positives = 225/392 (57%), Gaps = 27/392 (6%)

Query: 11 RVVRPLVAARRRAAACCALAACMLALAFAPAAARAERLKDLAQIQGVRDNPLIGYGLVVG 70
RV+R + AA +A L+ PA A R+KD+A +Q RDN LIGYGLVVG
Sbjct: 2 RVLRIIAAALVFSALPF--------LSTPPAQADTSRIKDIASLQAGRDNQLIGYGLVVG 53

Query: 71 LDGTGDQTMQTPFTTQTLANMLANLGISINNGSANGGGSSAMTNMQLKNVAAVMVTATLP 130
L GTGD +PFT Q++ ML NLGI+ G +N KN+AAVMVTA LP
Sbjct: 54 LQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQSN-----------AKNIAAVMVTANLP 102

Query: 131 PFARPGEAIDVTVSSLGNAKSLRGGTLLLTPLKGADGQVYALAQGNMAVGGAGASANGSR 190
PFA PG +DVTVSSLG+A SLRGG L++T L GADGQ+YA+AQG + V G A + +
Sbjct: 103 PFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQGALIVNGFSAQGDAAT 162

Query: 191 VQVNQLAAGRIAGGAIVERSVPNAVAQMNGVLQLQLNDMDYGTAQRIVSAVNS----SFG 246
+ + R+ GAI+ER +P+ L LQL + D+ TA R+ VN+ +G
Sbjct: 163 LTQGVTTSARVPNGAIIERELPSKFKDSV-NLVLQLRNPDFSTAVRVADVVNAFARARYG 221

Query: 247 AGTATALDGRTIQLTAPADSAQQVAFMARLQNLEVSPERAAAKVILNARTGSIVMNQMVT 306
A D + I + P + MA ++NL V + AKV++N RTG+IV+ V
Sbjct: 222 DPIAEPRDSQEIAVQKPRVA-DLTRLMAEIENLTVETD-TPAKVVINERTGTIVIGADVR 279

Query: 307 LQNCAVAHGNLSVVVNTQPVVSQPGPFSNGQTVVAQQSQIQLKQDNGSLRMVTAGANLAD 366
+ AV++G L+V V P V QP PFS GQT V Q+ I Q+ + + G +L
Sbjct: 280 ISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGSKV-AIVEGPDLRT 338

Query: 367 VVKALNSLGATPADLMSILQAMKAAGALRADL 398
+V LNS+G +++ILQ +K+AGAL+A+L
Sbjct: 339 LVAGLNSIGLKADGIIAILQGIKSAGALQAEL 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0286FLGFLGJ2273e-75 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 227 bits (579), Expect = 3e-75
Identities = 124/297 (41%), Positives = 173/297 (58%), Gaps = 15/297 (5%)

Query: 15 ALDVQGFDALRSKATAAAPREGVKMVAGQFDAMFTQMMLKSMRDATPSDGLLDSSSSKMY 74
A D Q + L++KA P ++ VA Q + MF QMMLKSMRDA P DGL S +++Y
Sbjct: 12 AWDAQSLNELKAKA-GEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDGLFSSEHTRLY 70

Query: 75 TSMLDQQLAQQMSS-KGIGVADALTKQLLRNANVAPDAQGEGGLAAMNALAKAYANSNGA 133
TSM DQQ+AQQM++ KG+G+A+ + KQ+ + ++ + Y N +
Sbjct: 71 TSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPEESTPAAPMKFPLETVVRYQNQALS 130

Query: 134 PGNGALAGTRGYSAASALTPPLKGNGNSAQADAFVEKMALAAQAASATTGIPARFIVGQA 193
P + + AF+ +++L AQ AS +G+P I+ QA
Sbjct: 131 ------------QLVQKAVPRNYDDSLPGDSKAFLAQLSLPAQLASQQSGVPHHLILAQA 178

Query: 194 ALESGWGKREIRGANGESSYNVFGIKATKGWTGRTVSAVTTEYVNGKPHRVVAQFRAYDS 253
ALESGWG+R+IR NGE SYN+FG+KA+ W G TTEY NG+ +V A+FR Y S
Sbjct: 179 ALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSS 238

Query: 254 YEHAMTDYANLLKNNPRYASVLNAGHNAEGFAHGMQKAGYATDPHYAKKLISIMQQI 310
Y A++DY LL NPRYA+V A +AE A +Q AGYATDPHYA+KL +++QQ+
Sbjct: 239 YLEALSDYVGLLTRNPRYAAVTTAA-SAEQGAQALQDAGYATDPHYARKLTNMIQQM 294


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0288FLGHOOKAP12314e-70 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 231 bits (591), Expect = 4e-70
Identities = 162/444 (36%), Positives = 253/444 (56%), Gaps = 12/444 (2%)

Query: 3 NTLMNLGVSGLNAALWGLTTTGQNISNAATPGYSVERPVYAEASGQYTSSGYLPQGVSTV 62
++L+N +SGLNAA L T NIS+ GY+ + + A+A+ + G++ GV
Sbjct: 1 SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVS 60

Query: 63 TVERQYNQYLSNQLNAAQTQGSSLSTYYTLVAQLNNYVGSPTAGIATAITNYFTGLQTVA 122
V+R+Y+ +++NQL AAQTQ S L+ Y +++++N + + T+ +AT + ++FT LQT+
Sbjct: 61 GVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLV 120

Query: 123 NNAADPSARQTAMSNAQTLASQLVAAGQQYSQLRQSVNSQLTDTVTQINSYTSQIAQLNE 182
+NA DP+ARQ + ++ L +Q Q + VN + +V QIN+Y QIA LN+
Sbjct: 121 SNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLND 180

Query: 183 QIA--SASSQGQPPNQLLDQRDLAVSKLSQLAGVQV-VQSNGNYSVFLSGGQPLVVGNAS 239
QI+ + G PN LLDQRD VS+L+Q+ GV+V VQ G Y++ ++ G LV G+ +
Sbjct: 181 QISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTA 240

Query: 240 YQLATVASPSDPSELTI-VSKGVAGSAQPGPTQYLPDVSLTGGALGGLLAFRSQTLDPAQ 298
QLA V S +DPS T+ G AG+ + +P+ L G+LGG+L FRSQ LD +
Sbjct: 241 RQLAAVPSSADPSRTTVAYVDGTAGNIE------IPEKLLNTGSLGGILTFRSQDLDQTR 294

Query: 299 AQLGALAVSFASQVNAQNALGVDMSGNPGGSLFAVGAPAVYANQNNTGSATLSVSFVDGT 358
LG LA++FA N Q+ G D +G+ G FA+G PAV N N G + + D +
Sbjct: 295 NTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDAS 354

Query: 359 QPTTSDYALSYDGAKYTLTDRATGSVVGTATPSSTPPTMTIGGLKLSLSSTPNAGDSFTV 418
+DY +S+D ++ +T R + T TP + + GL+L+ + TP DSFT+
Sbjct: 355 AVLATDYKISFDNNQWQVT-RLASNTTFTVTPDAN-GKVAFDGLELTFTGTPAVNDSFTL 412

Query: 419 LPTRGALDGFSLATANGSAIAAAS 442
P A+ + + + IA AS
Sbjct: 413 KPVSDAIVNMDVLITDEAKIAMAS 436



Score = 83.1 bits (205), Expect = 9e-19
Identities = 46/105 (43%), Positives = 66/105 (62%)

Query: 561 GTNDGRNALALSQLVNSKTMNNGTTTLTGAYAGYVNAIGNAASQLKASSAAQTALVGQIT 620
G +D RN AL L ++ G + AYA V+ IGN + LK SSA Q +V Q++
Sbjct: 441 GDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLS 500

Query: 621 QAQQSVSGVNQNEEAANLMQYQQLYQANAKVIQTANSVFQTVLGL 665
QQS+SGVN +EE NL ++QQ Y ANA+V+QTAN++F ++ +
Sbjct: 501 NQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0289FLAGELLIN424e-06 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 42.0 bits (98), Expect = 4e-06
Identities = 51/390 (13%), Positives = 110/390 (28%), Gaps = 12/390 (3%)

Query: 16 MNDQQAQIAQLYQQVSSGISLTTPADNPLAAAQAVQLSATSATLAQYTQNQTIVQTALQT 75
+N Q+ ++ +++SSG+ + + D+ A A + ++ L Q ++N + QT
Sbjct: 17 LNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNANDGISIAQT 76

Query: 76 EDTTLTSVNDVLNAAYQALMHAGDGGLSDSDRAALAAQIQGSRDHLLTLANTADGAGNYL 135
+ L +N+ L + + A +G SDSD ++ +IQ + + ++N G +
Sbjct: 77 TEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSNQTQFNGVKV 136

Query: 136 FAGFQPTTQPFSNKPGGGVTYAGDYGARAVQIADTRTVSQGDNGANVFMSVPFLGSLPVP 195
+ G +T + + S G +G NV
Sbjct: 137 LSQDNQMKIQVGANDGETIT---------IDLQKIDVKSLGLDGFNVNGPKEATVGDLKS 187

Query: 196 AAGASNTGTGTIGAVSITNPSDPTNTHQFTITFGGTAAAPTYTVTDNSVTPPPTTAAQAY 255
+ + + T + +T T A+
Sbjct: 188 SFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLT---TDDAENN 244

Query: 256 SSGQGINLGGQTVAVSGKPAVGDTFTVTPAPQAGTDVFATLDTVIAALKSPVGNSQTAST 315
++ T + A+ T G T
Sbjct: 245 TAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTIN 304

Query: 316 ALTNTMATASTKLMNTMTNVLTVQASVGGRLQEVKAMQAVTTTNTLQTTNSLSNLTDTNL 375
T+ A + T+Q+S V ++ + +
Sbjct: 305 GEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAV 364

Query: 376 PAAISQFLQLQNSLSAAQKAFVQMQNLSLF 405
+ + A V + ++F
Sbjct: 365 KGESKITVNGAEYTANAAGDKVTLAGKTMF 394


59BURPS1106A_0478BURPS1106A_0485N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_04782111.269756C4-dicarboxylate transport transcriptional
BURPS1106A_04796180.954419thiol-disulfide oxidoreductase
BURPS1106A_04804123.987934lipoprotein
BURPS1106A_04811123.349947thioesterase domain-containing protein
BURPS1106A_04821122.942274hypothetical protein
BURPS1106A_0483-1112.213454acetyltransferase
BURPS1106A_0484-1101.079770hypothetical protein
BURPS1106A_0485-1111.017552Mg chelatase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0478HTHFIS445e-156 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 445 bits (1145), Expect = e-156
Identities = 152/483 (31%), Positives = 231/483 (47%), Gaps = 47/483 (9%)

Query: 4 RLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDATGVIVSDIRLPGASG 63
++ +DD +R Q+L AG+DV + + I ++V+D+ +P +
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62

Query: 64 LELLAQCRERTPDVPVVLVTGHGDISMAVQAMRDGAYDFIEKPFAAERLTETVRRALERR 123
+LL + ++ PD+PV++++ A++A GAYD++ KPF L + RAL
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 124 ALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGKELI 183
+L ++GRS A++++ R++A + TD +++I G++G GKEL+
Sbjct: 123 K------RRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELV 176

Query: 184 ARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGGTLF 243
AR+LH+ RR+ PF+A+N A+P + ESE+FG+E GAFTGA R G+ E A GGTLF
Sbjct: 177 ARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLF 236

Query: 244 LDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDL 303
LDEI MP+ Q +LLRVLQ G +G PIR + R+VAA D+ + + G FR DL
Sbjct: 237 LDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDL 296

Query: 304 LYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNV 363
YRLNVV + LPPL +R EDI L HF + A + G + WPGNV
Sbjct: 297 YYRLNVVPLRLPPLRDRAEDIPDLVRHF-VQQAEKEGLDVKRFDQEALELMKAHPWPGNV 355

Query: 364 RELRNAADRFVLGVTEGIVG---------------------------------------- 383
REL N R + ++
Sbjct: 356 RELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQY 415

Query: 384 DAGPETDEHAEQSLKERVEQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGL 443
A + + E +I L T G AD L + + TL +K++ G+
Sbjct: 416 FASFGDALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGV 475

Query: 444 SAK 446
S
Sbjct: 476 SVY 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0479TYPE4SSCAGX280.025 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 27.8 bits (61), Expect = 0.025
Identities = 19/64 (29%), Positives = 31/64 (48%), Gaps = 8/64 (12%)

Query: 97 EFVAVAMNYDPPMYVANYAQTRQ------LPFKVALDDGSVAK-QFGNVQLTPTTFVIGK 149
++V A+ +P NY Q + +P ++ DDG+ F N+ L P FV+
Sbjct: 386 QYVHNALKRNPVPRNYNYYQAPEKRSKHIMPSEI-FDDGTFTYFGFKNITLQPAIFVVQP 444

Query: 150 DGKI 153
DGK+
Sbjct: 445 DGKL 448


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0483SACTRNSFRASE326e-04 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 31.8 bits (72), Expect = 6e-04
Identities = 20/83 (24%), Positives = 30/83 (36%), Gaps = 6/83 (7%)

Query: 47 GEALLVAQARDE--GIVGFVSVWEPERFVHHLYVAGTRLREGIGAALLRALPGW----PA 100
G+A + + G + S W + + VA ++G+G ALL W
Sbjct: 64 GKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHF 123

Query: 101 ARYRLKCLVRNERALAFYRAHGF 123
L+ N A FY H F
Sbjct: 124 CGLMLETQDINISACHFYAKHHF 146


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0485SYCECHAPRONE290.024 Gram-negative bacterial type III secretion SycE cha...
		>SYCECHAPRONE#Gram-negative bacterial type III secretion SycE

chaperone signature.
Length = 130

Score = 28.9 bits (64), Expect = 0.024
Identities = 22/84 (26%), Positives = 32/84 (38%), Gaps = 8/84 (9%)

Query: 158 ELYLPLPSAAEAALVPGVTVYGAADLPALCAHLADTPDGRLAPVAAPRLDALPAAATADL 217
+L L +P E + GV V C H+ + P G++ P LD T
Sbjct: 14 QLSLSIPDTIEPVI--GVKVG-----EFAC-HITEHPVGQILMFTLPSLDNNDEKETLLS 65

Query: 218 ADVIGQAGAKRALEVAAAGGHHML 241
++ Q K L GGH +L
Sbjct: 66 HNIFSQDILKPILSWDEVGGHPVL 89


60BURPS1106A_0820BURPS1106A_0825N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0820-1130.782505type IV pilin protein PilA
BURPS1106A_0821-2120.255896O-antigen polymerase family protein
BURPS1106A_08221122.190943hypothetical protein
BURPS1106A_08231122.123761hypothetical protein
BURPS1106A_08241133.095677hypothetical protein
BURPS1106A_0825-1103.153470TonB domain-containing protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0820BCTERIALGSPH414e-07 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 41.1 bits (96), Expect = 4e-07
Identities = 29/124 (23%), Positives = 44/124 (35%), Gaps = 23/124 (18%)

Query: 1 MRARGFTLIELMIVLAIVGVVAAYAIPAYQDYLARSRVGEGLALAAS--ARLAVAENAAS 58
MR RGFTL+E+M++L ++GV A + A+ SR A A+L +
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAFPA----SRDDSAAQTLARFEAQLRFVQQRGL 56

Query: 59 GNGFSGGYVSPPATRNVDSIRVDDDSGQIVV-----AFTTRVAAAGANTLVLVPSAPDQA 113
G G + V D Q +V A G + +P +
Sbjct: 57 QTGQFFG------------VSVHPDRWQFLVLEARDGADPAPADDGWSGYRWLPLRAGRV 104

Query: 114 DTPT 117
T
Sbjct: 105 ATSG 108


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0821PF06580290.047 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.1 bits (65), Expect = 0.047
Identities = 17/107 (15%), Positives = 41/107 (38%), Gaps = 14/107 (13%)

Query: 205 AALSALLSVGLALTVSRGPWLQVG-----------VMVVAGFWMAFA-QARRDPA--ASR 250
+ + +L+ + R WL++ +V+ W R A ++
Sbjct: 49 SLMGLVLTHAYRSFIKRQGWLKLNMGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTK 108

Query: 251 ARAWAIPVVLGVLFVAVNVAVRWANVHYHLGLAESAADRMRDAGQIA 297
A+ +P+ L ++F V V W+ +++ ++ D ++A
Sbjct: 109 PVAFTLPLALSIIFNVVVVTFMWSLLYFGWHFFKNYKQAEIDQWKMA 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0823RTXTOXINA270.047 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 26.9 bits (59), Expect = 0.047
Identities = 16/60 (26%), Positives = 25/60 (41%), Gaps = 8/60 (13%)

Query: 28 LGDFLKQGADAGNGGAGGIAGALGNLGGGGGAASAGSL-------LTPGSTGNVAGLLQF 80
L F K A + I+ L ++ G AA+ SL L TG ++G+L+
Sbjct: 354 LAAFHK-ETGAIDASLTTISTVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEA 412


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0825PF03544399e-07 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 39.2 bits (91), Expect = 9e-07
Identities = 18/97 (18%), Positives = 28/97 (28%), Gaps = 2/97 (2%)

Query: 18 AGCAAFAPRDAAKLECTMPVAAYPENAKPLERRATVLVRAMITASGNAENVTVTTSSRNA 77
+ L P YP A+ L V V+ +T G +NV + ++
Sbjct: 147 SKPVTSVASGPRALSRNQPQ--YPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPAN 204

Query: 78 AADRAAVDAMSRIACSQTPARGGEPYPFTLTRPFVFE 114
+R +AM R G E
Sbjct: 205 MFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTE 241


61BURPS1106A_0853BURPS1106A_0860N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_0853-3100.506871serine protease
BURPS1106A_0854-2121.090765hypothetical protein
BURPS1106A_0855013-1.047484hypothetical protein
BURPS1106A_0856114-0.075252carbon monoxide dehydrogenase family protein
BURPS1106A_0857012-0.562430multidrug efflux pump repressor protein BpeR
BURPS1106A_0858011-1.011975hypothetical protein
BURPS1106A_0859-2110.500697multidrug efflux periplasmic linker protein
BURPS1106A_0860-2110.398768inner membrane multidrug efflux protein BpeB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0853V8PROTEASE794e-18 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 78.5 bits (193), Expect = 4e-18
Identities = 38/207 (18%), Positives = 71/207 (34%), Gaps = 40/207 (19%)

Query: 81 QRRAAPQLPIDPDDP-----FYQFFRHFYGQIPGMGGGRQPQPDDQPSTSLGSGFIISAD 135
++R + + +D I Q + T + SG ++
Sbjct: 62 EQREHANVILPNNDRHQITDTTNGHYAPVTYI---------QVEAPTGTFIASGVVV-GK 111

Query: 136 GYILTNAHVIDGANVVTVKLTDKR-----------EYKA-KVVGADKQSDVAVLKIDA-- 181
+LTN HV+D + L + A ++ + D+A++K
Sbjct: 112 DTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNE 171

Query: 182 ------SGLPIVKIGDPAQSKVGQWVVAIGSPYGFDNTVTSGIISAKSRALPDENYTPFI 235
+ + + A+++V Q + G P +K + + +
Sbjct: 172 QNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMW---ESKGKITYLKGE--AM 226

Query: 236 QTDVPVNPGNSGGPLFNLNGEVIGINS 262
Q D+ GNSG P+FN EVIGI+
Sbjct: 227 QYDLSTTGGNSGSPVFNEKNEVIGIHW 253


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0857HTHTETR1262e-38 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 126 bits (317), Expect = 2e-38
Identities = 81/209 (38%), Positives = 115/209 (55%), Gaps = 1/209 (0%)

Query: 1 MARRTKEEALATRDRILDAAEHVFFEKGVSHTSLADIAQHAGVTRGAIYWHFASKSELFD 60
MAR+TK+EA TR ILD A +F ++GVS TSL +IA+ AGVTRGAIYWHF KS+LF
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 AMFDRVLLPIDELKAGT-GEPHADPLGRIREILIWCLLGAARDPQLRRVFSILFMKCEYV 119
+++ I EL+ + DPL +REILI L + + R + I+F KCE+V
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 120 ADMGPLLQRNREGMRDALRNIEADLAQGVANGQLPADLDTWRATLMLHTLVSGFVRDMLM 179
+M + Q R ++ IE L + LPADL T RA +++ +SG + + L
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 180 LPGEIDAERHAEKLVDGCFDMLRTSPAMR 208
P D ++ A V +M P +R
Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLR 209


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0859RTXTOXIND424e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.7 bits (98), Expect = 4e-06
Identities = 42/266 (15%), Positives = 80/266 (30%), Gaps = 75/266 (28%)

Query: 92 KIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQQYDDAVAAQGQAA 151
+++ A+ + A + + + + + + + L+ A++K + +A
Sbjct: 206 ELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAV 265

Query: 152 ADVGAGKAAV-------------------------------------------ETAQINL 168
++ K+ + +
Sbjct: 266 NELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQ 325

Query: 169 GYTDVVSPITGRV-GISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDI 227
+ + +P++ +V + T G V ++ TLM V + D + V + D +
Sbjct: 326 QASVIRAPVSVKVQQLKVHTEGGVVTTAE-TLMVIVPEDDTLEVTALVQNKDIGFINVG- 383

Query: 228 QSGRIK-------TEGPGAAKVTLILEDGKPYPERGKLQFSDVTVDQTTGSVT--IRAI- 277
Q+ IK G KV I D DQ G V I +I
Sbjct: 384 QNAIIKVEAFPYTRYGYLVGKVKNI--------------NLDAIEDQRLGLVFNVIISIE 429

Query: 278 -----FPNKQRVLLPGMFVRARIEEG 298
NK L GM V A I+ G
Sbjct: 430 ENCLSTGNKNIPLSSGMAVTAEIKTG 455



Score = 30.6 bits (69), Expect = 0.012
Identities = 20/122 (16%), Positives = 35/122 (28%), Gaps = 20/122 (16%)

Query: 1 MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQPVPVVSELPGRTSAY 60
R V Y ++ A L+ G+ E G +T + +
Sbjct: 55 RRPRLVAYFIMGFLVIAFILSVLGQVEIVATAN----GKLTHSGRSKEIKPIENSIVKEI 110

Query: 61 LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQN 120
+V EG V+ G L K+ A +++L +A+
Sbjct: 111 IVK----------------EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQ 154

Query: 121 AL 122
L
Sbjct: 155 IL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0860ACRIFLAVINRP12720.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1272 bits (3293), Expect = 0.0
Identities = 674/1035 (65%), Positives = 822/1035 (79%), Gaps = 2/1035 (0%)

Query: 1 MAKFFIDRPIFAWVIAIILMLAGVAAIFTLPIAQYPTIAPPSIQITANYPGASAKTVEDT 60
MA FFI RPIFAWV+AIILM+AG AI LP+AQYPTIAPP++ ++ANYPGA A+TV+DT
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTQVIEQQMSGLDNFLYMSSTSDDSGNATITITFAPGTNPDIAQVQVQNKLSLATPILPQ 120
VTQVIEQ M+G+DN +YMSSTSD +G+ TIT+TF GT+PDIAQVQVQNKL LATP+LPQ
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 121 VVQQLGLSVTKSSSSFLLVLAFNSEDGSMNKYDLANYVASHVKDPISRINGVGTVTLFGS 180
VQQ G+SV KSSSS+L+V F S++ + D+++YVAS+VKD +SR+NGVG V LFG+
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 QYAMRIWLDPTKLTNYGLTPVDVTSAISAQNVQIAGGQLGGTPAVPGTVLQATITEATLL 240
QYAMRIWLD L Y LTPVDV + + QN QIA GQLGGTPA+PG L A+I T
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 241 QTPEQFGNILLKVNQDGSQVRLKDVAQIGLGGETYNFDTKYNGQPTAALGIQLATNANAL 300
+ PE+FG + L+VN DGS VRLKDVA++ LGGE YN + NG+P A LGI+LAT ANAL
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 301 ATAKAVRAKIDEMSAYFPHGLVVKYPYDTTPFVRLSIEEVVKTLLEGIVLVFLVMYLFLQ 360
TAKA++AK+ E+ +FP G+ V YPYDTTPFV+LSI EVVKTL E I+LVFLVMYLFLQ
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 361 NLRATIIPTIAVPVVLLGTFAIMSMVGFSINVLSMFGLVLAIGLLVDDAIVVVENVERVM 420
N+RAT+IPTIAVPVVLLGTFAI++ G+SIN L+MFG+VLAIGLLVDDAIVVVENVERVM
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 421 AEEGLPPKEATRKAMGQITGALVGVALVLSAVFVPVAFSGGSVGAIYRQFSLTIVSAMVL 480
E+ LPPKEAT K+M QI GALVG+A+VLSAVF+P+AF GGS GAIYRQFS+TIVSAM L
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 481 SVLVALILTPALCATILKPIPQGHHEEKKGFFGWFNRTFNSSRDKYHVGVHHVIKRSGRW 540
SVLVALILTPALCAT+LKP+ HHE K GFFGWFN TF+ S + Y V ++ +GR+
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 541 LIIYLAVIVAVGLLFVRLPKSFLPDEDQGLMFVIVQTPSGSTQETTARTLANISDYLLTQ 600
L+IY ++ + +LF+RLP SFLP+EDQG+ ++Q P+G+TQE T + L ++DY L
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 601 EKDIVESAFTVNGFSFAGRGQNSGLVFVKLKDYSQRQSSDQKVQALIGRMFGRYAGYKDA 660
EK VES FTVNGFSF+G+ QN+G+ FV LK + +R + +A+I R +D
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 661 LVIPFNPPSIPELGTAAGFDFELTDNAGLGHDALMAARNQLLGMAAKDP-TLRGVRPNGL 719
VIPFN P+I ELGTA GFDFEL D AGLGHDAL ARNQLLGMAA+ P +L VRPNGL
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 720 NDTPQYKVDIDREKANALGVTADAIDQTFSIAWASKYVNNFLDTDGRIKKVYVQSDAPFR 779
DT Q+K+++D+EKA ALGV+ I+QT S A YVN+F+D GR+KK+YVQ+DA FR
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFID-RGRVKKLYVQADAKFR 779

Query: 780 MTPEDMNIWYVRNGSGGMVPFSAFATGHWTYGSPKLERYNGISAMEIQGQAAPGKSTGQA 839
M PED++ YVR+ +G MVPFSAF T HW YGSP+LERYNG+ +MEIQG+AAPG S+G A
Sbjct: 780 MLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDA 839

Query: 840 MTAMETLAKKLPTGIGYSWTGLSFQEIQSGSQAPILYAISILVVFLCLAALYESWSIPFS 899
M ME LA KLP GIGY WTG+S+QE SG+QAP L AIS +VVFLCLAALYESWSIP S
Sbjct: 840 MALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVS 899

Query: 900 VIMVVPLGVIGALLAATLRGLENDVFFQVGLLTTVGLSAKNAILIVEFARELQQTEKMGP 959
V++VVPLG++G LLAATL +NDV+F VGLLTT+GLSAKNAILIVEFA++L + E G
Sbjct: 900 VMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGV 959

Query: 960 IEAALEAARLRLRPILMTSLAFILGVMPLAISNGAGSASQHAIGTGVIGGMITATFLAIF 1019
+EA L A R+RLRPILMTSLAFILGV+PLAISNGAGS +Q+A+G GV+GGM++AT LAIF
Sbjct: 960 VEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIF 1019

Query: 1020 MIPMFFVKVRAVFSG 1034
+P+FFV +R F G
Sbjct: 1020 FVPVFFVVIRRCFKG 1034


62BURPS1106A_0880BURPS1106A_0885N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_08800123.300070carbohydrate ABC transporter ATP-binding
BURPS1106A_08810123.961602hypothetical protein
BURPS1106A_0882-1105.532048hypothetical protein
BURPS1106A_08830135.373429LysR family transcriptional regulator
BURPS1106A_08840135.328961esterase
BURPS1106A_08850135.090160major facilitator family transporter
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0880PF05272300.021 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.021
Identities = 14/35 (40%), Positives = 17/35 (48%)

Query: 50 VVFVGPSGCGKSTLMRMIAGLEEISGGELLIDGAK 84
VV G G GKSTL+ + GL+ S I K
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGK 633


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0881PF06776300.019 Invasion associated locus B
		>PF06776#Invasion associated locus B

Length = 214

Score = 29.5 bits (66), Expect = 0.019
Identities = 11/49 (22%), Positives = 15/49 (30%), Gaps = 2/49 (4%)

Query: 1 MKTGRRHFVRSVASASAALAAAAWSPARAAIDAPASPATALSLTPGRWS 49
+ + RR R+ A A A A A A+ G W
Sbjct: 38 LASCRRLARRNGARLMLAGAMAI--ALSFGWSDRADAQGAVRSVHGDWQ 84



Score = 28.7 bits (64), Expect = 0.038
Identities = 7/37 (18%), Positives = 13/37 (35%)

Query: 10 RSVASASAALAAAAWSPARAAIDAPASPATALSLTPG 46
+++ A L+ S R A A A ++
Sbjct: 25 KAIQMGPAELSPMLASCRRLARRNGARLMLAGAMAIA 61


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0884BLACTAMASEA300.018 Beta-lactamase class A signature.
		>BLACTAMASEA#Beta-lactamase class A signature.

Length = 286

Score = 29.8 bits (67), Expect = 0.018
Identities = 11/35 (31%), Positives = 15/35 (42%)

Query: 57 REDALFRFASVSKPIVSAAAMRAVAAGKLDLDASI 91
R D F S K ++ A + V AG L+ I
Sbjct: 57 RADERFPMMSTFKVVLCGAVLARVDAGDEQLERKI 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_0885TCRTETB363e-04 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 35.6 bits (82), Expect = 3e-04
Identities = 31/155 (20%), Positives = 59/155 (38%), Gaps = 5/155 (3%)

Query: 26 LLALATAGFITIVTEALPAGLLPLMGRDLRVSDALVGQLVTVYAAGSIVAAMPLVAATRG 85
L+ L F +++ E + LP + D A + T + + +
Sbjct: 16 LIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQ 75

Query: 86 MRRRPLLLAALAGFVVANTATAASPYYAPVLV-ARCVAGVSAGLLWALLAGYASRMVDAR 144
+ + LLL + + + +L+ AR + G A AL+ +R +
Sbjct: 76 LGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKE 135

Query: 145 QRGRAIAIAMLGAPVAMSVGI-PL-GTALGAALGW 177
RG+A ++G+ VAM G+ P G + + W
Sbjct: 136 NRGKAFG--LIGSIVAMGEGVGPAIGGMIAHYIHW 168


63BURPS1106A_1365BURPS1106A_1369N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_13652130.403286hypothetical protein
BURPS1106A_13662120.164913hypothetical protein
BURPS1106A_1367113-0.532244multidrug resistance protein MdtC
BURPS1106A_1368011-0.736131multidrug resistance protein MdtB
BURPS1106A_1369212-0.890626membrane fusion protein MdtA
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1365PF01540290.015 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

Length = 475

Score = 28.9 bits (64), Expect = 0.015
Identities = 26/84 (30%), Positives = 38/84 (45%), Gaps = 3/84 (3%)

Query: 13 RTGRALADLLLKQQDFEVTALVRRPDFA--LPGAKVVVADLTGDFSSAFN-GITHAIYAA 69
+ G+ AD LKQ + L + PD++ L +A+ T F A + G AI +
Sbjct: 35 KNGKEKADAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYGDYPAIISK 94

Query: 70 GSAESEGATEEEQIDRDAVARAAD 93
SA E A E+Q A + AD
Sbjct: 95 LSAAVENAKSEQQKVDQANKKIAD 118


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1367ACRIFLAVINRP7450.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 745 bits (1925), Expect = 0.0
Identities = 279/1104 (25%), Positives = 502/1104 (45%), Gaps = 100/1104 (9%)

Query: 3 LARPFITRPVATTLLALGIALAGLFAFVKLPVSPLPQVDFPTILVQASLPGASPETVATS 62
+A FI RP+ +LA+ + +AG A ++LPV+ P + P + V A+ PGA +TV +
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 63 VTSPLERHLGSIADVAEMTSMS-SVGNARIVLQFNLNRDIDGAARDVQAAINAARADLPA 121
VT +E+++ I ++ M+S S S G+ I L F D D A VQ + A LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 122 SLKSNPTYRKVNPADSPIMVVSLTS--KTASPAKLYDAASTVLQQSLSQIDGIGQVSLSG 179
++ + S +MV S + + D ++ ++ +LS+++G+G V L G
Sbjct: 121 EVQ-QQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFG 179

Query: 180 SANPAVRVELEPQALFHYGIGLEDVRAALASANANSPKGAIEAGP------HRYQLYTND 233
+ A+R+ L+ L Y + DV L N G + P +
Sbjct: 180 AQY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQT 238

Query: 234 QATKAAQYKDLVI-AYRNHAAVSLSDVSSVVDSVEDLRNLGLMNGERAVLVILYRSPGAN 292
+ ++ + + + + V L DV+ V E+ + +NG+ A + + + GAN
Sbjct: 239 RFKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGAN 298

Query: 293 IIDTIERVKAALPQLTAALPADIQVTPVLDRSRTIRASLADTEHTLIIAVSLVVMVVFLF 352
+DT + +KA L +L P ++V D + ++ S+ + TL A+ LV +V++LF
Sbjct: 299 ALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLF 358

Query: 353 LRNWRATLIPSVAVPISIVGTFGAMYLLGFSLNNLSLMALIVATGFVVDDAIVVLENIAR 412
L+N RATLIP++AVP+ ++GTF + G+S+N L++ +++A G +VDDAIVV+EN+ R
Sbjct: 359 LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVER 418

Query: 413 HI-ENGTPRLQAAFDGAREVGFTVLSISLSLVAVFLPILLMGGIVGRLFREFALTLSLAI 471
+ E+ P +A ++ ++ I++ L AVF+P+ GG G ++R+F++T+ A+
Sbjct: 419 VMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAM 478

Query: 472 AVSLVVSLTLTPMMCARLLPEAHAPRDE--GRVARWLERGFEWMQRGYERTLSWALRHPF 529
A+S++V+L LTP +CA LL A E G W F+ Y ++ L
Sbjct: 479 ALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTG 538

Query: 530 TILMTLVATIALNIALYIVVPKGFFPQQDTGLMIGGIQADQTTSFQAMKLRFTEMMRIIR 589
L+ +A + L++ +P F P++D G+ + IQ + + + ++
Sbjct: 539 RYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYL 598

Query: 590 ANP-----NVANVAGFT-GGAQTNSGFMFVALKDKPQR---KLSADQVIQQLRPQLAEVA 640
N +V V GF+ G N+G FV+LK +R + SA+ VI + + +L ++
Sbjct: 599 KNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 641 GARTFLQAAQDIRAGGRQSNAQYQFT-LLGDSTAELYKWGP-ILTEALQKRPELADVNSD 698
I G + ++ G L + +L A Q L V +
Sbjct: 659 DGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPN 718

Query: 699 QQQGGLEAMVTIDRATAARLGIKPAQIDNTLYDAFGQRQVSTIYNPLNQYHVVMEVAPQY 758
+ + + +D+ A LG+ + I+ T+ A G V+ + + ++ ++
Sbjct: 719 GLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKF 778

Query: 759 WQSPEMLKQIYISTSGGSASGVQTTNAAAGTYVATTARASTAGAAAQSAAAIAADSARNQ 818
PE + ++Y+ ++ G V + +V + R R
Sbjct: 779 RMLPEDVDKLYVRSANGEM--VPFSAFTTSHWVYGSPRLE-----------------RYN 819

Query: 819 ALNSIASSG--KSSASSGAAVSTSKSTMVPLSAIASFGPSTTPLAVNHQGLFVATTISFN 876
L S+ G SSG A++ ++
Sbjct: 820 GLPSMEIQGEAAPGTSSGDAMALMENLAS------------------------------K 849

Query: 877 LPPGVSLSKATQVIYQTMAEVGVPPTIQGSFQGTAQAFQESLKDQPILILAALAAVYIVL 936
LP G+ G + P L+ + V++ L
Sbjct: 850 LPAGIGY------------------DWTGMSYQERLSG----NQAPALVAISFVVVFLCL 887

Query: 937 GILYESYIHPVTILSTLPSAGVGALLGLLLFKTEFSIIALIGVILLIGIVKKNAIMMVDF 996
LYES+ PV+++ +P VG LL LF + + ++G++ IG+ KNAI++V+F
Sbjct: 888 AALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEF 947

Query: 997 AIDA-SRQGKSSFDAIHEACLLRFRPIMMTTMAALLGALPLAFGRGDGAEMRAPLGIAIA 1055
A D ++GK +A A +R RPI+MT++A +LG LPLA G G+ + +GI +
Sbjct: 948 AKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVM 1007

Query: 1056 GGLIVSQMLTLYTTPVVYLYMDRL 1079
GG++ + +L ++ PV ++ + R
Sbjct: 1008 GGMVSATLLAIFFVPVFFVVIRRC 1031



Score = 96.1 bits (239), Expect = 4e-22
Identities = 83/503 (16%), Positives = 167/503 (33%), Gaps = 25/503 (4%)

Query: 2 NLARPFITRPVATTLLALGIALAGLFAFVKLPVSPLPQVDFPTILVQASLP-GASPETVA 60
N + L+ I + F++LP S LP+ D L LP GA+ E
Sbjct: 528 NSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQ 587

Query: 61 TSVTSPLERHLGSIAD----VAEMTSMSSVGNAR----IVLQFNLNRDIDGAARDVQAAI 112
+ + +L + V + S G A+ + + +G +A I
Sbjct: 588 KVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVI 647

Query: 113 NAARADLPASLKSNPTYRKVNPADSPIMVVSLTSKT-----ASPAKLYDAASTVLQQSLS 167
+ A+ +L + + L A + +L +
Sbjct: 648 HRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQ 707

Query: 168 QIDGIGQVSLSGSAN-PAVRVELEPQALFHYGIGLEDVRAALASANANSPKGAIEAGPHR 226
+ V +G + ++E++ + G+ L D+ +++A +
Sbjct: 708 HPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRV 767

Query: 227 YQLYT---NDQATKAAQYKDLVIAYRNHAAVSLSDVSSVVDSVEDLRNLGLMNGERAVLV 283
+LY L + N V S ++ V L NG ++ +
Sbjct: 768 KKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHW-VYGSPRLERYNGLPSMEI 826

Query: 284 ILYRSPGANIIDTIERVKAALPQLTAALPADIQVTPVLDRSRTIRASLADTEHTLIIAVS 343
+PG + D A + L + LPA I S R S + I+
Sbjct: 827 QGEAAPGTSSGD----AMALMENLASKLPAGIGYD-WTGMSYQERLSGNQAPALVAISFV 881

Query: 344 LVVMVVFLFLRNWRATLIPSVAVPISIVGTFGAMYLLGFSLNNLSLMALIVATGFVVDDA 403
+V + + +W + + VP+ IVG A L + ++ L+ G +A
Sbjct: 882 VVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNA 941

Query: 404 IVVLENI-ARHIENGTPRLQAAFDGAREVGFTVLSISLSLVAVFLPILLMGGIVGRLFRE 462
I+++E + G ++A R +L SL+ + LP+ + G
Sbjct: 942 ILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNA 1001

Query: 463 FALTLSLAIAVSLVVSLTLTPMM 485
+ + + + ++++ P+
Sbjct: 1002 VGIGVMGGMVSATLLAIFFVPVF 1024



Score = 59.9 bits (145), Expect = 5e-11
Identities = 37/225 (16%), Positives = 84/225 (37%), Gaps = 4/225 (1%)

Query: 870 ATTISFNLPPGVSLSKATQVIYQTMAEV--GVPPTIQGS-FQGTAQAFQESLKDQPILIL 926
A + L G + + I +AE+ P ++ T Q S+ + +
Sbjct: 286 AAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLF 345

Query: 927 AALAAVYIVLGILYESYIHPVTILSTLPSAGVGALLGLLLFKTEFSIIALIGVILLIGIV 986
A+ V++V+ + ++ + +P +G L F + + + G++L IG++
Sbjct: 346 EAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLL 405

Query: 987 KKNAIMMVDFAIDASRQGKSSF-DAIHEACLLRFRPIMMTTMAALLGALPLAFGRGDGAE 1045
+AI++V+ + K +A ++ ++ M +P+AF G
Sbjct: 406 VDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGA 465

Query: 1046 MRAPLGIAIAGGLIVSQMLTLYTTPVVYLYMDRLRVWAEKRRDRR 1090
+ I I + +S ++ L TP + + +
Sbjct: 466 IYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGG 510


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1368ACRIFLAVINRP8010.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 801 bits (2071), Expect = 0.0
Identities = 284/1035 (27%), Positives = 498/1035 (48%), Gaps = 31/1035 (2%)

Query: 4 SRVFILRPVGTALLMAAIMLAGLVALRFLPLAALPEVDYPTIQVQTFYPGASPEVMTSSV 63
+ FI RP+ +L +M+AG +A+ LP+A P + P + V YPGA + + +V
Sbjct: 2 ANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTV 61

Query: 64 TAPLERQFGQMPSLNQMSSQS-SAGASVITLQFSLDLPLDIAEQEVQAAINAAGNLLPSD 122
T +E+ + +L MSS S SAG+ ITL F DIA+ +VQ + A LLP +
Sbjct: 62 TQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQE 121

Query: 123 LPAPPIYAKVNPADAPVITLAVTSKTLPLTQ--VQDLADTRLAMKISQVSGVGLVSLSGG 180
+ I + + ++ S TQ + D + + +S+++GVG V L G
Sbjct: 122 VQQQGIS-VEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 181 NRPAVRIQANPLALASYGLNLDDLRTTISNLNVNTPKGNFDGP------TRAYTINANDQ 234
A+RI + L Y L D+ + N G G +I A +
Sbjct: 181 QY-AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTR 239

Query: 235 LTSADQYNDAVV-AYKNGRPVMLTDVAKIVAGSENTKLGAWVDAEPAIILNVQRQPGANV 293
+ +++ + +G V L DVA++ G EN + A ++ +PA L ++ GAN
Sbjct: 240 FKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANA 299

Query: 294 IQTVDNVKAILPKLQESLPAALDVQIVTDRTTMIRAAVRDVQFELGLAVALVVLVMYLFL 353
+ T +KA L +LQ P + V D T ++ ++ +V L A+ LV LVMYLFL
Sbjct: 300 LDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 354 ANVYATIIPSLSVPLSLIGTLAVMYLSGFSLNNLSLMALTIATGFVVDDAIVMIENIARY 413
N+ AT+IP+++VP+ L+GT A++ G+S+N L++ + +A G +VDDAIV++EN+ R
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 414 -VEEGDSALEAALKGSKQIGFTIISLTVSLIAVLIPLLFMGDVVGRLFHEFAITLAVTIV 472
+E+ EA K QI ++ + + L AV IP+ F G G ++ +F+IT+ +
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 473 ISAVVSLTLVPMMCAKLLRHTPPPESHRFEAKVHGLIERV----IERYGVALQWVLDRQR 528
+S +V+L L P +CA LL+ E H + G + Y ++ +L
Sbjct: 480 LSVLVALILTPALCATLLK-PVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTG 538

Query: 529 ATLVVAVLTLALTALLYAVIPKGFFPTQDTGVIQAITQAPQSVSYGAMAERQQALAAEIL 588
L++ L +A +L+ +P F P +D GV + Q P + + + L
Sbjct: 539 RYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYL 598

Query: 589 KH--PDVVSLTSFIGVDGANITLNSGRMLINLKPRDERS---ESASDVIRSLQRQVANVT 643
K+ +V S+ + G + N+G ++LKP +ER+ SA VI + ++ +
Sbjct: 599 KNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIR 658

Query: 644 GISLYMQPVQDLTIDSTVSPTQYQFMLTS---PNPDEFATWVPKLVDRLKKEPS-LADVA 699
+ P I + T + F L D +L+ + P+ L V
Sbjct: 659 DGFVI--PFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVR 716

Query: 700 TDLQNSGKSVYIEIDRTSAARFGITPATVDNALYDAYGQRIVSTIFTQSNQYRVILESEP 759
+ +E+D+ A G++ + ++ + A G V+ + ++ ++++
Sbjct: 717 PNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADA 776

Query: 760 QMQHYTDSLNGIYLPSAGGGQVPLSAIATFRERPAPLLVSHLSQFPATTISFNLAPGASL 819
+ + + ++ +Y+ SA G VP SA T + + P+ I APG S
Sbjct: 777 KFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSS 836

Query: 820 GEAVKAIDAAERELGLPASFQTRFQGAALAFQASLSNQLFLILAAIVTMYIVLGVLYESY 879
G+A+ ++ +L PA + G + + S + L+ + V +++ L LYES+
Sbjct: 837 GDAMALMENLASKL--PAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESW 894

Query: 880 IHPITILSTLPSAGVGALLALMITGHDLDIIGIIGIVLLIGIVKKNAIMMIDFALEAERV 939
P++++ +P VG LLA + D+ ++G++ IG+ KNAI++++FA +
Sbjct: 895 SIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEK 954

Query: 940 EGKPPREAIYQACLLRFRPILMTTLAALLGAVPLIVGSGAGSELRQPLGIAIAGGLIVSQ 999
EGK EA A +R RPILMT+LA +LG +PL + +GAGS + +GI + GG++ +
Sbjct: 955 EGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSAT 1014

Query: 1000 VLTLFTTPVIYLGFD 1014
+L +F PV ++
Sbjct: 1015 LLAIFFVPVFFVVIR 1029


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1369RTXTOXIND484e-08 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 48.3 bits (115), Expect = 4e-08
Identities = 27/149 (18%), Positives = 57/149 (38%), Gaps = 16/149 (10%)

Query: 84 AARGEMPVVLNALGTVTPLANV-TVRTQLSGYLQAVSFQEGQIVKKGDVLAQIDPRP--- 139
+ G++ +V A G +T ++ + ++ + +EG+ V+KGDVL ++
Sbjct: 75 SVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEA 134

Query: 140 ----YQISLANAQGALARDEALLATARLDLKRYQTLVAQ---DSIAKQTADTQASLVKQY 192
Q SL A+ R + L + L+ L + +++++ SL+K+
Sbjct: 135 DTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKE- 193

Query: 193 EGTVQIDRAAIDSAKLNLAYARITAPVSG 221
Q + L + A
Sbjct: 194 ----QFSTWQNQKYQKELNLDKKRAERLT 218



Score = 38.3 bits (89), Expect = 5e-05
Identities = 33/182 (18%), Positives = 61/182 (33%), Gaps = 26/182 (14%)

Query: 141 QISLANAQGALARDEALLAT--ARLDLKRYQTLVAQDSIAKQTADTQASLVKQY-EGTVQ 197
+ ++ + L ++L+ + L A++ T + ++ + + T
Sbjct: 251 KHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDN 310

Query: 198 ID--RAAIDSAKLNLAYARITAPVSGRV-GLRQVDPGNYVTPSDT--------NGIVVIT 246
I + + + I APVS +V L+ G VT ++T + + V
Sbjct: 311 IGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTA 370

Query: 247 QLQPMSVIFTTSEDNLPAILKQVGAGGKLSVTAYNRNNTTPLETGV-LDTLDNQIDTATG 305
+Q + F AI+K V A+ L V LD D G
Sbjct: 371 LVQNKDIGFINVG--QNAIIK---------VEAFPYTRYGYLVGKVKNINLDAIEDQRLG 419

Query: 306 TV 307
V
Sbjct: 420 LV 421


64BURPS1106A_1636BURPS1106A_1644N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_16361112.749564fimbrial usher protein
BURPS1106A_16380121.722887type 1 pili protein CsuE
BURPS1106A_16370121.952513hypothetical protein
BURPS1106A_16392112.532402response regulator/sensor histidine kinase
BURPS1106A_16400112.332574capsular synthesis regulator component B
BURPS1106A_1641-1112.785141NodT family efflux transporter outer membrane
BURPS1106A_1642-290.929896drug:H+ antiporter-2 (DHA2) family protein
BURPS1106A_1643-3121.383634hypothetical protein
BURPS1106A_16440131.794934HlyD family secretion protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1636PF00577456e-150 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 456 bits (1175), Expect = e-150
Identities = 175/865 (20%), Positives = 282/865 (32%), Gaps = 103/865 (11%)

Query: 14 RRRAAAWAVGIAFAAAGHARAGETATLADSF------GRALPPV-------GGAAAHGTL 60
+ R A + V + A A A+A ++ F G GT
Sbjct: 20 KHRLAGFFVRLFVACAFAAQAPLSSA-ELYFNPRFLADDPQAVADLSRFENGQELPPGTY 78

Query: 61 YLELVVN-ALSTGRIVPVRYRDGIYYARA----GDLAQASVRTGAQP-------DALVDL 108
+++ +N R V D LA + T + DA V L
Sbjct: 79 RVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPL 138

Query: 109 -SRLDGVQVEYESAEQRLKLTVPPDWLPRQTLG--SPRLYDRTPAAVSFGLLFNYDVYAN 165
S + + + +QRL LT+P ++ + G P L+D A L NY+ N
Sbjct: 139 TSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINA----GLLNYNFSGN 194

Query: 166 SPT--LGTSYTSAWTEQRLFDRWGTVTNTGVYRRDYGGGAGGVGSNRYLRYDTFWRYSDQ 223
S +G + A+ + G Y GS ++ W D
Sbjct: 195 SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDI 254

Query: 224 DRLR-TYTAGDVITGALSWSSAVRLGGVSVERDFKVRPDIVTYPLPQFSGQAAVPTAVDL 282
LR T GD T + + G + D + PD P G A V +
Sbjct: 255 IPLRSRLTLGDGYTQGDIFDG-INFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTI 313

Query: 283 FINGSKTTTGQVNPGPFTMNNVPFINGAGEATVVTTDALGRQVATTIPFYVANTLLQKGL 342
NG V PGPFT+N++ +G+ V +A G T+P+ L ++G
Sbjct: 314 KQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGH 373

Query: 343 SDYSLSAGAMRRDYGIRSFSYGKFAASGTARHGLTDYLTLEGHVEGGERFALGGLGFDLG 402
+ YS++AG R + T HGL T+ G + +R+ G
Sbjct: 374 TRYSITAGEYRSGNAQQE---KPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKN 430

Query: 403 IGMFGVLGVAATQSRLAGASGRQY---------------------AFGYSYASQRF-SVS 440
+G G L V TQ+ Q+ GY Y++ + + +
Sbjct: 431 MGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFA 490

Query: 441 LQRIQRTNGFRDLS--------VYDLPANVAYRLVRSSTQATGALNLGALG----GTLGA 488
R NG+ + R Q T LG
Sbjct: 491 DTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQ 550

Query: 489 GYFDVRGADGARTRIANLSYTRPLWRRATLYASVNKTVGEHGVAAQLQLIV--PLG---- 542
Y+ D N ++ W TL S+ K + G L L V P
Sbjct: 551 TYWGTSNVDEQFQAGLNTAFEDINW---TLSYSLTKNAWQKGRDQMLALNVNIPFSHWLR 607

Query: 543 -------EPGVVTGALARDANNSFSERVQYSRSVPSDGGLGWNL--AYAGGGSHYQ---- 589
+ +++ D N + ++ D L +++ YAGGG
Sbjct: 608 SDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTG 667

Query: 590 QADATWRNRYFQAQGGVYGYGAGRGYARWGEVQGSVVVMDGAVLPANRVDDAFVLIDTQG 649
A +R Y A G + + V G V+ V ++D VL+ G
Sbjct: 668 YATLNYRGGYGNANIGYSHSDDIKQL--YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPG 725

Query: 650 RGGVPVRYENQLVGKTDGGGHLLVPWAPSYYAGKYEIDPLDLPSNVRVPIVERRVAVRDH 709
V ENQ +TD G+ ++P+A Y + +D L NV + V
Sbjct: 726 AKDAKV--ENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRG 783

Query: 710 GGALVTFPIRRIVCAQIALVDAAGRPVAIGSRVLHEESGETALVGWQGETYLEGLSALNH 769
F R + + +P+ G+ V E S + +V G+ YL G+
Sbjct: 784 AIVRAEFKARVG-IKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGK 842

Query: 770 LRVR--TPDGRTCRATFAADVDAAQ 792
++V+ + C A + ++ Q
Sbjct: 843 VQVKWGEEENAHCVANYQLPPESQQ 867


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1639HTHFIS631e-12 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 63.3 bits (154), Expect = 1e-12
Identities = 30/122 (24%), Positives = 50/122 (40%), Gaps = 10/122 (8%)

Query: 440 RVLVVDDQEMNRIVLRYQLDALGHHARLCASGDEALRALGTAAYDVVLTDCRMPGMDGIA 499
+LV DD R VL L G+ R+ ++ R + D+V+TD MP +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 500 LTAAIRAH-PDARVRATPIVGVTALVSDAEHARCVDAGMTLCIGKP----TTLDALERAL 554
L I+ PD P++ ++A + + + G + KP + + RAL
Sbjct: 65 LLPRIKKARPD-----LPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 555 VE 556
E
Sbjct: 120 AE 121


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1640HTHFIS553e-11 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 55.2 bits (133), Expect = 3e-11
Identities = 41/159 (25%), Positives = 65/159 (40%), Gaps = 13/159 (8%)

Query: 5 VLIADDHPLVLLGVRHMLAGMG-DVSIVGEAHDPAGLLALLAATPCDIVITDFAMPEQPA 63
+L+ADD + + L+ G DV I A L +AA D+V+TD MP+
Sbjct: 6 ILVADDDAAIRTVLNQALSRAGYDVRITSNAAT---LWRWIAAGDGDLVVTDVVMPD--- 59

Query: 64 ADGLAMLTAIRDGYPSVRVIVLTMLDNPVLMHTMRQAGALAVLSKRGDLDEL----PRAL 119
+ +L I+ P + V+V++ + + + GA L K DL EL RAL
Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRAL 119

Query: 120 AAVYQGRPFVGTHAGAAGGGAMRGTDAPRQLSPREIEVV 158
A + + G + G A Q R + +
Sbjct: 120 AEPKRRPS--KLEDDSQDGMPLVGRSAAMQEIYRVLARL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1642TCRTETB1022e-25 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 102 bits (256), Expect = 2e-25
Identities = 71/331 (21%), Positives = 140/331 (42%), Gaps = 20/331 (6%)

Query: 41 AFMEVLDTTIVNVALPHIAGTMSASYDEATWTLTSYLVANGIVLPISGFLGRLLGRKRYF 100
+F VL+ ++NV+LP IA + W T++++ I + G L LG KR
Sbjct: 23 SFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLL 82

Query: 101 VLCIVAFTICSFLCGIATDLGQLIVF-RVLQGLFGGGLQPNQQSIILDTF-PPEQRNRAF 158
+ I+ S + + L++ R +QG G P +++ + P E R +AF
Sbjct: 83 LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGA-GAAAFPALVMVVVARYIPKENRGKAF 141

Query: 159 SISAVAIVVAPVLGPTLGGWITDNFSWRWVFLLNVPIGVLTSLAVIQLVEDPPWKRGRAR 218
+ + + +GP +GG I W +LL +P+ +T + V L++ K R +
Sbjct: 142 GLIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPM--ITIITVPFLMKLLK-KEVRIK 196

Query: 219 GLSIDYIGITLIAIGLGCLQVMLDRGEDEDWFASTFIRTFAVLTVAGLVGATFWLLYAKK 278
G D GI L+++G+ + F +++ +F +++V + +
Sbjct: 197 G-HFDIKGIILMSVGIVFFML----------FTTSYSISFLIVSVLSFLIFVKHIRKVTD 245

Query: 279 PVVDLSCLKDRNFALGCVTIATFAVVLYGSAVLVPQLAQQRLGYTAMLAG-LVLSPGALL 337
P VD K+ F +G + + G +VP + + + G +++ PG +
Sbjct: 246 PFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMS 305

Query: 338 ITLEIPIVSKLMPYVQTRFLVCFGFLLLAAS 368
+ + I L+ +++ G L+ S
Sbjct: 306 VIIFGYIGGILVDRRGPLYVLNIGVTFLSVS 336


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1644RTXTOXIND999e-25 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 99.1 bits (247), Expect = 9e-25
Identities = 63/414 (15%), Positives = 134/414 (32%), Gaps = 91/414 (21%)

Query: 51 KRPGKKPLVVLAIIVVLLLVGAFVW-WFATRNQVSTDDA--YTDGNAITIAPKVSGYVVA 107
+ P + ++A ++ LV AF+ V+T + G + I P + V
Sbjct: 50 ETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKE 109

Query: 108 LAIDDNVYVHRGDLLLVIDKRDYQAQVDAARAQLGLAQAQLDAAQVQLDIA------HVQ 161
+ + + V +GD+LL + +A ++ L A+ + Q+ ++
Sbjct: 110 IIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELK 169

Query: 162 FPAQYRQAQA---QIEAAQASFRQALAAYERQHAVDARATSQQAIDVADAQRLTADANVA 218
P + ++ + ++ + ++ Q + + +D A+RLT A +
Sbjct: 170 LPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQ-----KYQKELNLDKKRAERLTVLARIN 224

Query: 219 TARAQA----------------------------RTASLVPQQIRQAQTAVEQRRQQVLQ 250
+ ++R ++ +EQ ++L
Sbjct: 225 RYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILS 284

Query: 251 AQA-----------------------------QLEAAQLALSYCEVRAPSDGWITRRNVQ 281
A+ +L + +RAP + + V
Sbjct: 285 AKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVH 344

Query: 282 -LGSFLQAGAALFAIVTPQ---LWVTANFKESQLERMRAGDRVSVSVDAYP---NLELHG 334
G + L IV P+ L VTA + + + G + V+A+P L G
Sbjct: 345 TEGGVVTTAETLMVIV-PEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVG 403

Query: 335 HVDSIQLGSGSRFSAFPPENATGNFVKIVQRVPVKIAIDGGLPRDPPLGIGLSV 388
V +I L + + G ++ + G ++ PL G++V
Sbjct: 404 KVKNINLDA-------IEDQRLGLVFNVIISIEENCLSTGN--KNIPLSSGMAV 448


65BURPS1106A_1766BURPS1106A_1773N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1766114-0.591550EmrB/QacA family drug resistance transporter
BURPS1106A_1767015-1.705523multidrug resistance protein
BURPS1106A_1768016-1.692854NodT family efflux transporter outer membrane
BURPS1106A_1769119-2.541234MarR family transcriptional regulator
BURPS1106A_1770118-2.639943hypothetical protein
BURPS1106A_1771018-2.779134GTP-binding protein TypA
BURPS1106A_1772-118-2.9609392-oxoglutarate dehydrogenase E1
BURPS1106A_1773-216-2.289224dihydrolipoamide succinyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1766TCRTETB1358e-37 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 135 bits (341), Expect = 8e-37
Identities = 84/396 (21%), Positives = 159/396 (40%), Gaps = 16/396 (4%)

Query: 27 VFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRIGQVRLF 86
F +VL+ + NV++P I+ D WV T+F + +I + G L+D++G RL
Sbjct: 23 SFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLL 82

Query: 87 LASIILFVISSWMCGLAPT-LPFLLASRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALA 145
L II+ S + + + L+ +R +QGA A L ++ P+ A
Sbjct: 83 LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFG 142

Query: 146 LWSMTTLIAPVAGPILGGWISDNYSWPWIFYVNIPVGIAAAAVTWMIYRSRESAVRRAPI 205
L + GP +GG I+ W ++ IP+ I V +++ ++ +
Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIHWSYLL--LIPM-ITIITVPFLMKLLKKEVRIKGHF 199

Query: 206 DGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIVVLALTALIAFAFFVVWELTAEHPVVD 265
D G+ L+ + G + ML F ++ + + ++++F FV P VD
Sbjct: 200 DIKGIILMSV--GIVFFML--------FTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVD 249

Query: 266 LSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAG-LVMAPVGFFAILL 324
L + F G + + +G G + ++P ++ + + G +++ P I+
Sbjct: 250 PGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIF 309

Query: 325 SPLTGKFLSRTDPRYIATAAFLTFALCFWMRSRYTTGVDEWSLMAPTFVQGIAMAGFFIP 384
+ G + R P Y+ ++ F S + + FV G ++
Sbjct: 310 GYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSFTKTV 368

Query: 385 LVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF 420
+ +I S L A L NF + G G +I
Sbjct: 369 ISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIV 404


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1767RTXTOXIND711e-15 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 71.4 bits (175), Expect = 1e-15
Identities = 36/270 (13%), Positives = 85/270 (31%), Gaps = 28/270 (10%)

Query: 94 ADSQVALQQAEANLAQTVRQVRGLYVNDDQYRAQVALRQSDLS--------------KAQ 139
+ Q Q E NL + + + ++Y + +S L
Sbjct: 196 STWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVL 255

Query: 140 DDLRRRLAVAQTGAVSQEEISHARDAVKAAQASLDAAGQQLASNRALTANTTVADHPNVL 199
+ + + V + ++ + +A+ Q + L N+
Sbjct: 256 EQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKN-EILDKLRQT--TDNIG 312

Query: 200 AAAAKVRDAYLNNARNTLPAPVTGYVAKRSVQ-VGQRVSPGTPLMSVVPLNAV-WVDANF 257
++ + + APV+ V + V G V+ LM +VP + V A
Sbjct: 313 LLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALV 372

Query: 258 KEVQLKHMRIGQPVELTADIYGSSVKYHGKVIGFSAGTGAAFSLLPAQNATGNWIKVVQR 317
+ + + +GQ + + + + +G ++G + + +V
Sbjct: 373 QNKDIGFINVGQNAIIKVEAFPYTR--YGYLVG-------KVKNINLDAIEDQRLGLVFN 423

Query: 318 LPVRVELDPKELKEHPLRIGLSMQVDVDIK 347
+ + +E + + + M V +IK
Sbjct: 424 VIISIEENCLSTGNKNIPLSSGMAVTAEIK 453



Score = 47.1 bits (112), Expect = 8e-08
Identities = 32/207 (15%), Positives = 72/207 (34%), Gaps = 28/207 (13%)

Query: 29 VIAIAAIAYGLYYLLVARFHETTDDAYVNGNVV------QITPQVTGTVIAVKADDTQTV 82
++A + + + +++ + A NG + +I P V + + ++V
Sbjct: 59 LVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESV 118

Query: 83 KSGDPLVVLDPADSQVALQQAEANLAQT---------------VRQVRGLYVNDDQYRAQ 127
+ GD L+ L ++ + +++L Q + ++ L + D+ Y
Sbjct: 119 RKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQN 178

Query: 128 VA----LRQSDLSKAQ-DDLRRRLAVAQ-TGAVSQEEISHARDAVKAAQASLDAAGQQLA 181
V+ LR + L K Q + + + + E + + +L
Sbjct: 179 VSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLD 238

Query: 182 SNRALTANTTVADHPNVLAAAAKVRDA 208
+L +A H VL K +A
Sbjct: 239 DFSSLLHKQAIAKH-AVLEQENKYVEA 264


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1771TCRTETOQM1715e-48 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 171 bits (435), Expect = 5e-48
Identities = 102/435 (23%), Positives = 172/435 (39%), Gaps = 62/435 (14%)

Query: 5 LRNIAIIAHVDHGKTTLVDQLLRQSGTFRENQQVAE--RVMDSNDIEKERGITILAKNCA 62
+ NI ++AHVD GKTTL + LL SG E V + D+ +E++RGITI +
Sbjct: 3 IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS 62

Query: 63 VEYEGTHINIVDTPGHADFGGEVERVLSMVDSVLLLVDAVEGPMPQTRFVTKKALALGLK 122
++E T +NI+DTPGH DF EV R LS++D +LL+ A +G QTR + +G+
Sbjct: 63 FQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP 122

Query: 123 PIVVINKIDRPGARIDWV-------------INQTFDLFDKLGATE----EQLDFPIV-- 163
I INKID+ G + V I Q +L+ + T EQ D I
Sbjct: 123 TIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGN 182

Query: 164 -----------------YASGLNGY---ASLDP-----AARDGDMRPLFEAILQHVPVRP 198
+ SL P A + + L E I
Sbjct: 183 DDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSST 242

Query: 199 ADPDAPLQLQITSLDYSTYVGRIGVGRITRGRIKPGQPVVMRFGPEGDVLNRKINQVLSF 258
+ L ++ ++YS R+ R+ G + V R + + KI ++ +
Sbjct: 243 HRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSV--RISEKEKI---KITEMYTS 297

Query: 259 QGLERVQVDSAEAGDIVLINGIEDVGIGATICAVEAPEALPMITVDEPTLTMNFLVNSSP 318
E ++D A +G+IV++ E + + + + + I P L +
Sbjct: 298 INGELCKIDKAYSGEIVILQN-EFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQ 356

Query: 319 LAGREGKFVTSRQIRDRLMKELNHNVALRVKDTGDETVFEVSGRGELHLTILVENMRRE- 377
+ D L++ V E + +S G++ + + ++ +
Sbjct: 357 QREMLLDALLEISDSDPLLR-------YYVDSATHEII--LSFLGKVQMEVTCALLQEKY 407

Query: 378 GYELAVSRPRVVMQE 392
E+ + P V+ E
Sbjct: 408 HVEIEIKEPTVIYME 422



Score = 34.1 bits (78), Expect = 0.002
Identities = 17/100 (17%), Positives = 32/100 (32%), Gaps = 1/100 (1%)

Query: 387 RVVMQEVDGVKHEPYELLTVDLEDEHQGGVMEELGRRKGEMLDMVSDGRGRTRLEYRIPA 446
V+++ EPY + E+ + + ++D L IPA
Sbjct: 525 EQVLKKAGTELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQL-KNNEVILSGEIPA 583

Query: 447 RGLIGFQSEFLTLTRGTGLMSHIFDSYAPVKEGSVGERRN 486
R + ++S+ T G + Y V + R
Sbjct: 584 RCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRR 623


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1773RTXTOXIND290.027 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.4 bits (66), Expect = 0.027
Identities = 8/83 (9%), Positives = 26/83 (31%), Gaps = 3/83 (3%)

Query: 48 EVPAPAAGVLAQVLQNDGDTVVADQVIATID---TEAKAGAAAAAAGAADVQPAAAPVAA 104
E+ ++ +++ +G++V V+ + EA ++ A ++ + +
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 105 PAPAAQPAAAAASSTAAASPAAS 127
+ S
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVS 180


66BURPS1106A_1783BURPS1106A_1790N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_17831142.469331hypothetical protein
BURPS1106A_1784-2130.699148hypothetical protein
BURPS1106A_1785-2112.234467pilin family protein
BURPS1106A_1786-1111.933701peptidase A24A, prepilin type IV
BURPS1106A_17871122.683581TadE family protein
BURPS1106A_17882123.280146pilus assembly protein CpaB
BURPS1106A_17891133.239661type II/III secretion system protein
BURPS1106A_17902123.724738hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1783cloacin457e-07 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 44.7 bits (105), Expect = 7e-07
Identities = 33/117 (28%), Positives = 51/117 (43%), Gaps = 1/117 (0%)

Query: 30 GGSGTISKGLDGSGSGSGGGNAISTTGGSGSGGTSGAGGSGSGGSGSSGSTGGLSGGGGS 89
G+ + S ++G +G G G S G S GGSGSG G +G +GGG
Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-IHWGGGSGHGNGGGNG 69

Query: 90 TSGGGSTSGGGSTSGGTSTSSSINALGTIAGNTGGIISGAGSTVSGLGTVVGSQTLP 146
SGGGS +GG ++ + AL T + AG+ + + ++ + P
Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKGP 126



Score = 40.5 bits (94), Expect = 2e-05
Identities = 33/123 (26%), Positives = 46/123 (37%), Gaps = 2/123 (1%)

Query: 38 GLDGSGSGSGGGNAI-STTGGSGSGGTSGAGGSGSGGSGSSGSTGGLSGGG-GSTSGGGS 95
G DG G +G + + GG G G GSG S + GG SG G G G
Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62

Query: 96 TSGGGSTSGGTSTSSSINALGTIAGNTGGIISGAGSTVSGLGTVVGSQTLPGVNPQTTQA 155
+GGG+ + G + + N A G + + GL + + L A
Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122

Query: 156 LGG 158
L G
Sbjct: 123 LKG 125



Score = 34.7 bits (79), Expect = 0.001
Identities = 34/125 (27%), Positives = 52/125 (41%), Gaps = 4/125 (3%)

Query: 55 TGGSGSGGTSGAGGSGSGGSGSSGSTGGLSGGGGSTSGGGSTSGGGSTSGGTSTSSSINA 114
+GG G G +GA S +G GL GGG++ G G +S GG+ +
Sbjct: 2 SGGDGRGHNTGA---HSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58

Query: 115 LGTIAGNTGGIISGAGSTVSGLGTVVGSQTLPGVNPQTTQALGGVVQSL-GGAVSALGSG 173
G SG GS G + V + G +T GG+ S+ GA+SA +
Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118

Query: 174 VTSGI 178
+ + +
Sbjct: 119 IMAAL 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1786PREPILNPTASE534e-11 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 53.3 bits (128), Expect = 4e-11
Identities = 31/124 (25%), Positives = 52/124 (41%), Gaps = 10/124 (8%)

Query: 4 LFSIGFFFAWAAAVAIADCRDRRIPNELVLVGLAAVIIFTVCRQNPFGTTLSGALIGGAV 63
+ A+ D +P++L L L ++F + F +L A+IG
Sbjct: 134 TLAALLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNL--LGGF-VSLGDAVIGAMA 190

Query: 64 GLVSLFPFFAL-------RVMGAADVKVFAVLGAWCGLSALPRLWVVASVAAGVHALALM 116
G + L+ + MG D K+ A LGAW G ALP + +++S+ + L+
Sbjct: 191 GYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMGIGLI 250

Query: 117 LLTR 120
LL
Sbjct: 251 LLRN 254


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1789BCTERIALGSPD1382e-37 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 138 bits (348), Expect = 2e-37
Identities = 58/249 (23%), Positives = 111/249 (44%), Gaps = 11/249 (4%)

Query: 151 VQVDVRVVEFSRSVLKQAGLNFFKQNNGFTFGSFAPAGLASVTGGG----TSSMSVSANI 206
V V+ + E + G+ + +N G T + + +++ G S+
Sbjct: 347 VLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLA 406

Query: 207 PIASAFN-LVVGSATRGLFADLSILEANNLARVLAQPTLVALSGQSASFLAGGEIPVPVP 265
S+FN + G L+ L ++ +LA P++V L A+F G E+PV
Sbjct: 407 SALSSFNGIAAGFYQGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTG 466

Query: 266 QSLGT-----ISIDWKPYGVGLTLTPTVLSPRRIALKVAPESSQLDFVHSITINGVTVPA 320
+ +++ K G+ L + P + + L++ E S + S + +
Sbjct: 467 SQTTSGDNIFNTVERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAAS-STSSDLGAT 525

Query: 321 LTTRRADTTVELGDGESFAIGGLIDRETTSNVDKVPFLGDLPIIGTFFKHLSYQQNDKEL 380
TR + V +G GE+ +GGL+D+ + DKVP LGD+P+IG F+ S + + + L
Sbjct: 526 FNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNL 585

Query: 381 VIIVTPHLV 389
++ + P ++
Sbjct: 586 MLFIRPTVI 594


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1790HTHFIS385e-05 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 38.3 bits (89), Expect = 5e-05
Identities = 11/63 (17%), Positives = 26/63 (41%)

Query: 79 AALRVSHPGLPIVALGSLGEPESALAALRAGVRDFIDFSAPAEDALRITRGLLDHVGDQP 138
++ + P LP++ + + +A+ A G D++ + + I L +P
Sbjct: 67 PRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRRP 126

Query: 139 SRH 141
S+
Sbjct: 127 SKL 129


67BURPS1106A_1794BURPS1106A_1799N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1794-292.227679hypothetical protein
BURPS1106A_1795-2101.448086hypothetical protein
BURPS1106A_1796-1100.991273hypothetical protein
BURPS1106A_1797-111-0.470858sigma-54 interaction domain/Fis family
BURPS1106A_1798-210-0.870853hypothetical protein
BURPS1106A_1799-112-1.006677RNA chaperone Hfq
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1794PYOCINKILLER320.003 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 32.1 bits (72), Expect = 0.003
Identities = 30/86 (34%), Positives = 38/86 (44%), Gaps = 3/86 (3%)

Query: 214 LMNQLKLAPAVRTEIRNDATRIAAAARARQRA-LARPGAPGAAASAGATLAASAAGSNGG 272
MN L A A + R AAA A+++A AA A T A A GS
Sbjct: 203 RMNTLTAAKASIEAAAANKAREQAAAEAKRKAEEQARQQ--AAIRAANTYAMPANGSVVA 260

Query: 273 AAAGKGAVAGAGASASGAAATAAAAA 298
AAG+G + A +AS A A + A A
Sbjct: 261 TAAGRGLIQVAQGAASLAQAISDAIA 286


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1797HTHFIS2973e-98 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 297 bits (763), Expect = 3e-98
Identities = 130/475 (27%), Positives = 204/475 (42%), Gaps = 53/475 (11%)

Query: 19 ADIVDRVARCMSSFDVEVIRADN-EELSAERTAMRPSLAIISVSMIE-SGAAFLRTWQAE 76
A I + + +S +V N L A L + V M + + L +
Sbjct: 13 AAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFDLLPRIKKA 72

Query: 77 -IGMPVVWVGA--------------ARDHDPSLYPPEYSHILPLDFTCAELRGMISKLAV 121
+PV+ + A A D+ P P + + ++ + +++
Sbjct: 73 RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPK--PFDLTELIGIIGRA------LAEPKR 124

Query: 122 QLRAHAAKALEPSTLVAHSDCMQALLQEVDTFADCDTNVLLHGETGVGKERIAQLLHEKH 181
+ + + LV S MQ + + + D +++ GE+G GKE +A+ LH+ +
Sbjct: 125 RPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHD-Y 183

Query: 182 SRYGMGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVGTHKGYFEQAAGGTLFLDEVGDL 241
+ G FV +N AIP L ES FGH KG+FTGA G FEQA GGTLFLDE+GD+
Sbjct: 184 GKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243

Query: 242 PLYQQVKLLRVLEDGAVLRIGATAPVKVDFRLVAASNKKLPQLVKDGLFRADLYYRLAVI 301
P+ Q +LLRVL+ G +G P++ D R+VAA+NK L Q + GLFR DLYYRL V+
Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRLNVV 303

Query: 302 ELSIPSLEERGPVDKIALFKSFVASIVGEDRLAALPELPYWLAEAVADSYFPGNVRELRN 361
L +P L +R D L + FV E + E + +PGNVREL N
Sbjct: 304 PLRLPPLRDR-AEDIPDLVRHFVQQAEKEGL--DVKRFDQEALELMKAHPWPGNVRELEN 360

Query: 362 LAERVGV------------------------TVRQTGGWDTARLQRLIAHARSAAQPAPA 397
L R+ + + + + + +
Sbjct: 361 LVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFG 420

Query: 398 ESAPDVFVDRSKWDMTERNRVIAALDANGWRRQDTAQHLGISRKVLWEKMRKYQI 452
++ P + E ++AAL A + A LG++R L +K+R+ +
Sbjct: 421 DALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGV 475


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1798IGASERPTASE280.044 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 27.7 bits (61), Expect = 0.044
Identities = 19/108 (17%), Positives = 32/108 (29%), Gaps = 9/108 (8%)

Query: 113 LFQQKAFWRVIRTASEARAEAVYRDFAKQSETLAVNELQAAKLESQKALTDRQIAVA--- 169
++A V + + T K E K T++ V
Sbjct: 1067 EVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVT 1126

Query: 170 ------QERASRLQADLSIAREQRAAVATRQKDKLDETVALREQKSER 211
QE++ +Q ARE V ++ T A EQ ++
Sbjct: 1127 SQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKE 1174


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1799cloacin290.017 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 28.9 bits (64), Expect = 0.017
Identities = 25/85 (29%), Positives = 26/85 (30%), Gaps = 7/85 (8%)

Query: 76 GRGPRAGGAHGGGGRPGGREGGGHGPYGSHG----GSREPRGDGGGYGAREPRGDGGYGS 131
GRG G G GG G G G S G P G G G G GG G
Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG---IHWGGGSGH 62

Query: 132 RESRGDGGYGSREPRGDGGYGSREP 156
G+G G G P
Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAP 87


68BURPS1106A_1883BURPS1106A_1894N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_18833145.288854peptidase A24A, prepilin type IV
BURPS1106A_18842134.991127pilus assembly protein CpaB
BURPS1106A_18854164.282594type II/III secretion system protein
BURPS1106A_18864135.084432lipoprotein
BURPS1106A_18873124.453246CpaE protein
BURPS1106A_18881124.225864type IV pilus assembly protein
BURPS1106A_1889-1124.811496type II secretion system protein
BURPS1106A_1890-1113.291265type II secretion system protein
BURPS1106A_1891093.195522hypothetical protein
BURPS1106A_18921111.896821TadE family protein
BURPS1106A_18941112.434144TadE family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1883PREPILNPTASE320.001 Type IV prepilin cysteine protease (C20) family sig...
		>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family

signature.
Length = 290

Score = 31.7 bits (72), Expect = 0.001
Identities = 31/145 (21%), Positives = 50/145 (34%), Gaps = 12/145 (8%)

Query: 20 LVASWTLASLALADLRTRRLA---TFAVALVGALYAALALVGAPGDGGFASHAALGAAA- 75
L+ +W L +L DL L T + G L+ L + GD + A
Sbjct: 138 LLLTWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMAGYLVLWS 197

Query: 76 -FALGAAMFRAGWIAGGDVKLAAVVFLWAGPAHAWPVAFAIGVGGLAVGAVCIAAGRAPR 134
+ + + GD KL A + W G V + G +G I
Sbjct: 198 LYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSLVGAFMGIGLILLRNH-- 255

Query: 135 VLAWFAPARGVPYGVALAAGGLLAV 159
++ +P+G LA G +A+
Sbjct: 256 -----HQSKPIPFGPYLAIAGWIAL 275


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1885BCTERIALGSPD1443e-39 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 144 bits (364), Expect = 3e-39
Identities = 68/283 (24%), Positives = 116/283 (40%), Gaps = 16/283 (5%)

Query: 170 VVQTLKPYLRQQEALVNRLTLARPIQVHLRVRITEVDRNITQQLGINWSALGA------- 222
+V + E ++ +L + RP QV + I EV LGI W+ A
Sbjct: 322 IVTAAPDVMNDLERVIAQLDIRRP-QVLVEAIIAEVQDADGLNLGIQWANKNAGMTQFTN 380

Query: 223 SGNFVGGLFNGRTLFDTASKAFDLSPSGAFSVVGGFHTSRYSIDG--VLDALDQEGLITM 280
SG + G ++ S A S G Y + +L AL +
Sbjct: 381 SGLPISTAIAGANQYNKDGTVSSSLAS-ALSSFNGIAAGFYQGNWAMLLTALSSSTKNDI 439

Query: 281 LAEPNLTAISGQTASFLAGGEFPIPVAQDTTGA----ITIQFKPYGVSLDFTPTVLADNR 336
LA P++ + A+F G E P+ TT T++ K G+ L P + +
Sbjct: 440 LATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVERKTVGIKLKVKPQINEGDS 499

Query: 337 ISLKVRPEVSEIDPTNSVTTGSIKVPALTVRRVDTTVELSSGQSFAIGGLLQSKSSDVLA 396
+ L++ EVS + S T+ + R V+ V + SG++ +GGLL SD
Sbjct: 500 VLLEIEQEVSSVADAASSTSSDLGA-TFNTRTVNNAVLVGSGETVVVGGLLDKSVSDTAD 558

Query: 397 ELPGLARLPVLGKLFSSRNYLNDKTEVVVIVTPYIVQPANPGE 439
++P L +PV+G LF S + K +++ + P +++ +
Sbjct: 559 KVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRDRDEYR 601


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1887HTHFIS340.001 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 33.7 bits (77), Expect = 0.001
Identities = 29/165 (17%), Positives = 52/165 (31%), Gaps = 20/165 (12%)

Query: 22 GARLVAIVADAASDEVIRNLIADQAMTGAQVARGGIDDAIALMRDLSHGPQHLLVDVSGA 81
GA ++ DAA V+ ++ + + ++ DV
Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYD--VRITSNAATLWRWIA--AGDGDLVVTDVV-- 56

Query: 82 AMP----LSDLARLADVCDPSVNVIVIGERNDVGLFRSMLRIGVRDYLVKPL----TVEL 133
MP L R+ P + V+V+ +N G DYL KP + +
Sbjct: 57 -MPDENAFDLLPRIKKA-RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGI 114

Query: 134 VHRALSAADPNAAARAGKAIGFVGARGGVGVTSIAVALARHLADR 178
+ RAL+ + + + G S A+ + R
Sbjct: 115 IGRALAEPKRRPSKLEDDSQDGMPLVG----RSAAMQEIYRVLAR 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1888PF05272300.034 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 29.7 bits (66), Expect = 0.034
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 4/50 (8%)

Query: 302 IVISGGTGSGKTTLLNAL---SHFIDSHERIVTIEDAAELQLQQPHVVSL 348
+V+ G G GK+TL+N L F D+H I T +D+ E Q+ L
Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYE-QIAGIVAYEL 647


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1891SYCDCHAPRONE310.005 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 30.7 bits (69), Expect = 0.005
Identities = 20/83 (24%), Positives = 32/83 (38%)

Query: 38 SVAESALAAGDAELAATLFERALKADPRSLPAQVGLGDAMYQTGELARAGVLYAQAAAAA 97
S+A + +G E A +F+ D +GLG G+ A Y+ A
Sbjct: 41 SLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMD 100

Query: 98 PDDPRAQLGLARVALRERHLDDA 120
+PR A L++ L +A
Sbjct: 101 IKEPRFPFHAAECLLQKGELAEA 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1894PYOCINKILLER320.004 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 31.7 bits (71), Expect = 0.004
Identities = 30/132 (22%), Positives = 49/132 (37%), Gaps = 4/132 (3%)

Query: 40 RVAAARNELQNAADAAALAGAASLEAGAGAPAWAAAASAAAAALSLNASDGAALSSGDVQ 99
A A+ + + A A AA+ A PA + + AA + + GAA + +
Sbjct: 226 AAAEAKRKAEEQARQQAAIRAANTYA---MPANGSVVATAAGRGLIQVAQGAASLAQAIS 282

Query: 100 TGYWNVTGVPAGLEPTTLAPGEYDVPAVQATVTRAPNQNGGPLSLLMGGLLGLVGTPAAA 159
V G P+ +A G + T + +Q + +G +G P +
Sbjct: 283 DAI-AVLGRVLASAPSVMAVGFASLTYSSRTAEQWQDQTPDSVRYALGMDAAKLGLPPSV 341

Query: 160 TAVAVAGAPATV 171
AVA A TV
Sbjct: 342 NLNAVAKASGTV 353


69BURPS1106A_1902BURPS1106A_1910N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_1902-1102.110600multidrug efflux operon transciptional regulator
BURPS1106A_19030101.590457periplasmic multidrug efflux lipoprotein
BURPS1106A_19040110.546744multidrug efflux protein
BURPS1106A_19055111.033437outer membrane efflux protein OprA
BURPS1106A_1906514-0.571233hypothetical protein
BURPS1106A_1907414-0.924924hypothetical protein
BURPS1106A_19081130.846581hypothetical protein
BURPS1106A_19091141.434929fimbrial protein
BURPS1106A_19100121.817380fimbrial usher protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1902HTHTETR1175e-35 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 117 bits (295), Expect = 5e-35
Identities = 53/210 (25%), Positives = 100/210 (47%), Gaps = 4/210 (1%)

Query: 1 MARKTREESLNTKNRILDAAELVLLEKGVGQTAMADIAEAAGMSRGAVYGHFNGKIEVCV 60
MARKT++E+ T+ ILD A + ++GV T++ +IA+AAG++RGA+Y HF K ++
Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60

Query: 61 AVCDRAFSRAVEGFDLSDERPA---LATLRLAASHYLHQCGEPGSMQRVLEILYMKCEQS 117
+ + + S E + L+ LR H L + ++EI++ KCE
Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120

Query: 118 EENAPLMRRRALYELQTLRIAKALLRRAVAAGELDASLDVHLAGVYLLSLLEGIFGSMIW 177
E A + + + L++ + L+ + A L A L A + + + G+ + ++
Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180

Query: 178 TTRLRGDRWRDAEAMLDAGVDTLRASPALR 207
+ D ++A + ++ P LR
Sbjct: 181 APQSF-DLKKEARDYVAILLEMYLLCPTLR 209


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1903RTXTOXIND402e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 39.8 bits (93), Expect = 2e-05
Identities = 20/133 (15%), Positives = 40/133 (30%), Gaps = 5/133 (3%)

Query: 67 EVRARVAGIVTARTYEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKARAAHLAALDKR 126
E++ IV +EG+ V++G VL ++ +A +L +AR
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILS 157

Query: 127 RRYDELVRDRAVSERDHTEALADERQAKAAVASASAELA-----RAQLQLDYATVTAPID 181
R + + E + + + + + Q +L+ A
Sbjct: 158 RSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERL 217

Query: 182 GRARRALVTEGAL 194
R E
Sbjct: 218 TVLARINRYENLS 230



Score = 34.4 bits (79), Expect = 7e-04
Identities = 18/100 (18%), Positives = 39/100 (39%), Gaps = 10/100 (10%)

Query: 102 KAARDAAAGALEKARAAHLAALDKRRRYDELVRDRAVSERDHTEALADERQAKAAVASAS 161
LE+ + L+A ++ + +L + E L RQ + +
Sbjct: 265 VNELRVYKSQLEQIESEILSAKEEYQLVTQLFK---------NEILDKLRQTTDNIGLLT 315

Query: 162 AELARAQLQLDYATVTAPIDGR-ARRALVTEGALVGQDQA 200
ELA+ + + + + AP+ + + + TEG +V +
Sbjct: 316 LELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAET 355


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1904ACRIFLAVINRP10790.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 1079 bits (2791), Expect = 0.0
Identities = 516/1032 (50%), Positives = 701/1032 (67%), Gaps = 6/1032 (0%)

Query: 1 MARFFIDRPVFAWVISLFIMLGGIFAIRALPVAQYPDIAPPVVSLYATYPGASAQVVEES 60
MA FFI RP+FAWV+++ +M+ G AI LPVAQYP IAPP VS+ A YPGA AQ V+++
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 61 VTAVIEREMNGVPGLLYTSATS-SAGQASLSLTFKQGVSADLAAVDVQNRLKIVEARLPE 119
VT VIE+ MNG+ L+Y S+TS SAG +++LTF+ G D+A V VQN+L++ LP+
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 120 PVRRDGISIEKAADNAQIIVSLTSEDGRLSGVELGEYASANVLQALRRVEGVGKVQFWGA 179
V++ GIS+EK++ + ++ S++ + ++ +Y ++NV L R+ GVG VQ +GA
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGA 180

Query: 180 EYAMRIWPDPVKMAALGLTASDIASAVRAHNARVTIGDVGRSAVPDSAPIAATVLADAPL 239
+YAMRIW D + LT D+ + ++ N ++ G +G + + A+++A
Sbjct: 181 QYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRF 240

Query: 240 TTPDAFGAIALRARADGSTLYLRDVARIEFGGNDYNYPSFVNGKTATGMGIKLAPGSNAV 299
P+ FG + LR +DGS + L+DVAR+E GG +YN + +NGK A G+GIKLA G+NA+
Sbjct: 241 KNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANAL 300

Query: 300 ATEKRVRATMEELAKFFPPGVKYQIPYETASFVRVSMSKVVTTLVEAGVLVFAVMFLFMQ 359
T K ++A + EL FFP G+K PY+T FV++S+ +VV TL EA +LVF VM+LF+Q
Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQ 360

Query: 360 NFRATLIPTLVVPVALLGTFGAMLAAGFSINVLTMFGMVLAIGILVDDAIVVVENVERLM 419
N RATLIPT+ VPV LLGTF + A G+SIN LTMFGMVLAIG+LVDDAIVVVENVER+M
Sbjct: 361 NMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 420 VEEKLPPYEATVKAMKQISGAIVGITVVLTSVFVPMAFFGGAVGNIYRQFAFALAVSIGF 479
+E+KLPP EAT K+M QI GA+VGI +VL++VF+PMAFFGG+ G IYRQF+ + ++
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 480 SAFLALSLTPALCATLLKPVADDHHE-KDGFFGWFNRFVARSTHRYTRRVGRVLERPLRW 538
S +AL LTPALCATLLKPV+ +HHE K GFFGWFN S + YT VG++L R+
Sbjct: 481 SVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRY 540

Query: 539 LVVYGALTAAAALLITKLPAAFLPDEDQGNFMVMVIRPQGTPLAETMQSVRRVEEYVRTH 598
L++Y + A +L +LP++FLP+EDQG F+ M+ P G T + + +V +Y +
Sbjct: 541 LLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKN 600

Query: 599 SPSAY--TFALGGYNLYGEGPNGGMIFVTMKDWKERKRARDQVQAIIAEINAHFAGTPNT 656
+ F + G++ G+ N GM FV++K W+ER + +A+I +
Sbjct: 601 EKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDG 660

Query: 657 MVFAINMPALPDLGLTGGFDFRLQDRGGLGYGAFVAAREKLLAEGRKDPV-LTDLMFAGT 715
V NMPA+ +LG GFDF L D+ GLG+ A AR +LL + P L + G
Sbjct: 661 FVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGL 720

Query: 716 QDAPQLKLDIDRAKASALGVSMEEINATLAVMFGSDYIGDFMHGSQVRRVIVQADGRHRL 775
+D Q KL++D+ KA ALGVS+ +IN T++ G Y+ DF+ +V+++ VQAD + R+
Sbjct: 721 EDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRM 780

Query: 776 DAADVTKLRVRNAKGEMVPLAAFATLHWTMGPPQLTRYNGFPSFTINGAASAGHSSGEAM 835
DV KL VR+A GEMVP +AF T HW G P+L RYNG PS I G A+ G SSG+AM
Sbjct: 781 LPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 836 AAIERIASTLPAGTGYAWSGQSYEERLSGAQAPMLFALSVLVVFLALAALYESWSIPFAV 895
A +E +AS LPAG GY W+G SY+ERLSG QAP L A+S +VVFL LAALYESWSIP +V
Sbjct: 841 ALMENLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSV 900

Query: 896 MLVVPLGVIGAVAGVTLRGMPNDIYFKVGLIATIGLSAKNAILIVEVAKDLVAQR-MSLA 954
MLVVPLG++G + TL ND+YF VGL+ TIGLSAKNAILIVE AKDL+ + +
Sbjct: 901 MLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960

Query: 955 DAALEAARLRLRPIVMTSLAFGVGVLPLAFATGAASGAQIAIGTGVLGGVISATLFAIFL 1014
+A L A R+RLRPI+MTSLAF +GVLPLA + GA SGAQ A+G GV+GG++SATL AIF
Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020

Query: 1015 VPLFFVCVGRVF 1026
VP+FFV + R F
Sbjct: 1021 VPVFFVVIRRCF 1032


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1905RTXTOXIND330.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 33.3 bits (76), Expect = 0.002
Identities = 18/104 (17%), Positives = 34/104 (32%), Gaps = 2/104 (1%)

Query: 382 APRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTAFREV--ADALAARDQIDAQLA 439
P L LP +N + +V I Q +E+ A R + A++
Sbjct: 165 LPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARIN 224

Query: 440 AQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESGQEL 483
+ + + RL + +L+ + E+ EL
Sbjct: 225 RYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNEL 268


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_1910PF005776680.0 Outer membrane usher protein FimD
		>PF00577#Outer membrane usher protein FimD

Length = 878

Score = 668 bits (1725), Expect = 0.0
Identities = 223/842 (26%), Positives = 349/842 (41%), Gaps = 60/842 (7%)

Query: 2 FMLAAGSHARATEFNASFLSIDGRNDVDLSQFAQADYTLPGTYLLDVQVNDVFFGLQPIE 61
F A + FN FL+ D + DLS+F PGTY +D+ +N+ + + +
Sbjct: 36 FAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVT 95

Query: 62 FVAHDDGQGARACVAPELVAQFGLKKSLVENLPRTMGGRCADLASL-DGVTIRYQKGEGR 120
F D QG C+ +A GL + V + C L S+ T + G+ R
Sbjct: 96 FNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQR 155

Query: 121 LKITIAQAALEFADASYLPPERWSDGVDGAMLDYRVLANANHAFGRGAQQNNAVQAYGTI 180
L +TI QA + Y+PPE W G++ +L+Y + N R ++
Sbjct: 156 LNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYN--FSGNSVQNRIGGNSHYAYLNLQS 213

Query: 181 GANWGAWRFRGDYQAQ-TRAGGAVYAERAFRFNQLYAYRALPSIRSTLSFGEIYVDSDIF 239
G N GAWR R + + + ++ ++ + R + +RS L+ G+ Y DIF
Sbjct: 214 GLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIF 273

Query: 240 STFSMSGVAMKSDDRMLPPSMRGYAPLVTGVARTNAIVKVMQDSRVLYMTKVSPGAFALS 299
+ G + SDD MLP S RG+AP++ G+AR A V + Q+ +Y + V PG F ++
Sbjct: 274 DGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIN 333

Query: 300 NLN-TSVQGTLDVVVEEEDGTVQRFQVATAAVPFLAREGQLRYKTAIGQPRTFGGAGITP 358
++ G L V ++E DG+ Q F V ++VP L REG RY G+ R+ P
Sbjct: 334 DIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKP 393

Query: 359 WFGFAEAAYGLPFDVTVYGGLIAASGYTSVAFGVGRDFGRFGALSADVTHARATLWWNGR 418
F + +GLP T+YGG A Y + FG+G++ G GALS D+T A +TL +
Sbjct: 394 RFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTL-PDDS 452

Query: 419 TKRGNSYRINYSKHVDALDADVRFFGYRFSERDYTNFQQFSGDPTASGL----------- 467
G S R Y+K ++ +++ GYR+S Y NF +
Sbjct: 453 QHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVK 512

Query: 468 ----------ANGKQRYSAMLSKRFGDTST-YFSYDQTIYW-ARPSDRRIGVTLTRAFSL 515
N + + ++++ G TST Y S YW D + L AF
Sbjct: 513 PKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFED 572

Query: 516 GALKSVNLGFSAFRTQGAGGGGNQVSLTATLPLGER-----------QTLTSSVSAGEGG 564
+ L +S + G ++L +P + + S+S G
Sbjct: 573 I---NWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNG 629

Query: 565 TSVNAGYLYDGA---NGRTYQLYGGTTDGRASANASLRQRTPSYQ-----LTAQASTVAN 616
N +Y N +Y + G G + S T +Y+ S ++
Sbjct: 630 RMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYS-HSD 688

Query: 617 AYASASLEVDGSFVATRYGVTAHANGNAGDTRLLVSTDGVPGVPLS-GSYARTNARGYAV 675
V G +A GVT DT +LV G + + RT+ RGYAV
Sbjct: 689 DIKQLYYGVSGGVLAHANGVTLGQPL--NDTVVLVKAPGAKDAKVENQTGVRTDWRGYAV 746

Query: 676 IDGVSPYNVYDATVSVEKLGLDTDVTNPIQRTVLTDGAIGYIRFNAARGRNVFVTLTGDG 735
+ + Y + L + D+ N + V T GAI F A G + +TLT +
Sbjct: 747 LPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNN 806

Query: 736 GAPVPFGASVQDAATGKELGIVGEAGAAYLTQVQPRAKLVVRAGAKTICT---PAALPDT 792
P+PFGA V + + GIV + G YL+ + K+ V+ G + LP
Sbjct: 807 K-PLPFGAMVTS-ESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPE 864

Query: 793 LQ 794
Q
Sbjct: 865 SQ 866


70BURPS1106A_2058BURPS1106A_2065N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2058920-3.653805hypothetical protein
BURPS1106A_20591019-3.671961HlyD family secretion protein
BURPS1106A_20601119-3.831556ABC transporter permease
BURPS1106A_20611219-3.922150sulfotransferase domain-containing protein
BURPS1106A_20621219-3.777946hypothetical protein
BURPS1106A_20631219-3.585749type I secretion target repeat-containing
BURPS1106A_2064529-5.421852outer membrane efflux protein
BURPS1106A_2065234-6.964230ompA family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2058SYCDCHAPRONE330.005 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 33.0 bits (75), Expect = 0.005
Identities = 21/126 (16%), Positives = 45/126 (35%), Gaps = 3/126 (2%)

Query: 898 LAPDDADAVLLRAELALDTGDFDEALSQFERLREQRPDAPESYANLIPALAALERRDDAI 957
++ D + + A +G +++A F+ L + L A+ + D AI
Sbjct: 31 ISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAI 90

Query: 958 AALQRALELNSKHPGALNNGVQFYLRTQQYDKA---MELAQRYVGAHGELASAHTMCGLV 1014
+ ++ K P + + L+ + +A + LAQ + E T +
Sbjct: 91 HSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRVSSM 150

Query: 1015 YHNLKA 1020
+K
Sbjct: 151 LEAIKL 156


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2059RTXTOXIND2743e-89 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 274 bits (702), Expect = 3e-89
Identities = 94/439 (21%), Positives = 204/439 (46%), Gaps = 14/439 (3%)

Query: 41 SALGLEEASIAPARRAAALIPTVMLALLIVLVLWATFFKIDIIAAGQGKVIPSTTVQQLS 100
+ L L E ++ R A ++ L++ + + +++I+A GK+ S +++
Sbjct: 44 AHLELIETPVSRRPRLVAYF---IMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIK 100

Query: 101 TLEGGIVRELLVREGQIVKKGQPLVRLDPVVAQGAVTEQAATREGLMASIARLQAEADGK 160
+E IV+E++V+EG+ V+KG L++L + A+ + ++ R Q +
Sbjct: 101 PIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSI 160

Query: 161 ----------ATPLYPAGLKPEIVSEEEHVRAQRAEALNSTIEVLQQQRAAKQAEAADYR 210
Y + E V + ++ + + K+AE
Sbjct: 161 ELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVL 220

Query: 211 GRIPQYVNNQHLLDDQIQRMLPLVGVGSVAPNEITNLQRERGNLAAQIITTREGAAQASA 270
RI +Y N + ++ L+ ++A + + + + ++ + Q +
Sbjct: 221 ARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIES 280

Query: 271 QIAEASHKIEEKISTFRSEAREELARKQVQLQALEGTLSGKQDILDRTLIRSPVNGIVKT 330
+I A + + F++E ++L + + L L+ ++ ++IR+PV+ V+
Sbjct: 281 EILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQ 340

Query: 331 LYITTIGGVASPGKSVIDIVPTNDSLLIEARIQPQDIAYIRVGDDAKVRITAFDSGALGS 390
L + T GGV + ++++ IVP +D+L + A +Q +DI +I VG +A +++ AF G
Sbjct: 341 LKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGY 400

Query: 391 LDAKVELISPDSQADERSGSLYYKVQVRTHSSVVATQVGDLNILPGMVAEVDVITGRRTI 450
L KV+ I+ D+ D+R G L + V + + ++T ++ + GM ++ TG R++
Sbjct: 401 LVGKVKNINLDAIEDQRLG-LVFNVIISIEENCLSTGNKNIPLSSGMAVTAEIKTGMRSV 459

Query: 451 MSYILRPIVRGMSRAMSER 469
+SY+L P+ ++ ++ ER
Sbjct: 460 ISYLLSPLEESVTESLRER 478


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2063RTXTOXINA471e-06 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 47.3 bits (112), Expect = 1e-06
Identities = 24/78 (30%), Positives = 38/78 (48%)

Query: 2958 AGADTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDMLVVNGDNIAHFSTPGSYWD 3017
G DT++G +G D L GG GND ++G G + L GG G+D V G+++A G +
Sbjct: 762 KGNDTLSGGNGDDQLYGGDGNDKLIGVAGNNYLNGGDGDDEFQVQGNSLAKNVLFGGKGN 821

Query: 3018 GGIMMGGEINTLQFDANN 3035
+ + L +
Sbjct: 822 DKLYGSEGADLLDGGEGD 839



Score = 45.7 bits (108), Expect = 3e-06
Identities = 28/77 (36%), Positives = 36/77 (46%), Gaps = 8/77 (10%)

Query: 2959 GADTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDML--------VVNGDNIAHFS 3010
G D + G+ G D L G GNDT+ G G D L GG GND L + GD F
Sbjct: 745 GDDLIEGNDGNDRLYGDKGNDTLSGGNGDDQLYGGDGNDKLIGVAGNNYLNGGDGDDEFQ 804

Query: 3011 TPGSYWDGGIMMGGEIN 3027
G+ ++ GG+ N
Sbjct: 805 VQGNSLAKNVLFGGKGN 821



Score = 39.6 bits (92), Expect = 3e-04
Identities = 22/60 (36%), Positives = 30/60 (50%), Gaps = 1/60 (1%)

Query: 2961 DTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDMLVVNGDNIAHFSTPG-SYWDGG 3019
D G+ G D++ G GND + G+ G D L GG G+D L N G +Y +GG
Sbjct: 738 DIFHGADGDDLIEGNDGNDRLYGDKGNDTLSGGNGDDQLYGGDGNDKLIGVAGNNYLNGG 797



Score = 38.4 bits (89), Expect = 5e-04
Identities = 20/43 (46%), Positives = 23/43 (53%)

Query: 2957 TAGADTVTGSSGRDMLNGGAGNDTIVGNGGVDVLEGGGGNDML 2999
T AD GS D+ +G G+D I GN G D L G GND L
Sbjct: 725 TTRADKFFGSKFTDIFHGADGDDLIEGNDGNDRLYGDKGNDTL 767


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2065OMPADOMAIN1139e-32 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 113 bits (284), Expect = 9e-32
Identities = 59/180 (32%), Positives = 89/180 (49%), Gaps = 12/180 (6%)

Query: 78 QYQVRF--LGGLAYRGYWADSACRDIAARYADAAGLGVIAVAPCNPSDVAAPLPERVELP 135
Q+ + R ++ R+ V+A AP +V L
Sbjct: 163 QWTNNIGDAHTIGTRP-DNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTK---HFTLK 218

Query: 136 TDTLFAFDKGGFEDISADGRRQLGDLVASIKAKILSINHLIVTGYTDRLGSDEHNARLSS 195
+D LF F+K + +G+ L L + + ++V GYTDR+GSD +N LS
Sbjct: 219 SDVLFNFNK---ATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSE 275

Query: 196 ERARTVADYMIAEGIPAAKITAVGRGAADPVV--VCNNGEQ-PELIRCLQKNRRVEIRIK 252
RA++V DY+I++GIPA KI+A G G ++PV C+N +Q LI CL +RRVEI +K
Sbjct: 276 RRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVK 335


71BURPS1106A_2161BURPS1106A_2165N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2161-1102.198357TetR family transcriptional regulator
BURPS1106A_2162-292.701052RND family efflux transporter MFP subunit
BURPS1106A_2163-282.528791AcrB/AcrD/AcrF family protein
BURPS1106A_21640113.893049NodT family efflux transporter outer membrane
BURPS1106A_21650151.566879MerR family transcriptional regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2161HTHTETR617e-14 TetR bacterial regulatory protein HTH signature.
		>HTHTETR#TetR bacterial regulatory protein HTH signature.

Length = 215

Score = 61.2 bits (148), Expect = 7e-14
Identities = 41/203 (20%), Positives = 76/203 (37%), Gaps = 10/203 (4%)

Query: 5 RLTREQSKDLTRERLLSAAHAIFTKKGYVAASVEDIASAAGYTRGAFYSNFRSKAELLIE 64
R T++++++ TR+ +L A +F+++G + S+ +IA AAG TRGA Y +F+ K++L E
Sbjct: 3 RKTKQEAQE-TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61

Query: 65 LLKRDHEEAEADLQKIFE--SGGTREQMEA---HALEYYSQFFRNNPAFLLWGEAKLQAT 119
+ + + G + H LE R +
Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121

Query: 120 RDAKFRARFNEFVKEKRDRFTHYILTFAERVGTPLLLPADVLALGLMSLCDGVQSYHAAD 179
A + E DR + E P L A+ + G+
Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFA 181

Query: 180 PRHVTGDAAQQVLAGFFARVVLA 202
P+ D ++ A + ++L
Sbjct: 182 PQSF--DLKKE--ARDYVAILLE 200


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2162RTXTOXIND371e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.7 bits (85), Expect = 1e-04
Identities = 30/200 (15%), Positives = 59/200 (29%), Gaps = 32/200 (16%)

Query: 1 MNRSGSRAALLIGVALIAAACHRKEAAPSAPRPVVAVPAQADGAAAAVSLPGEIQPRYAT 60
+ SR L+ ++ + +VA A+G EI+P
Sbjct: 49 IETPVSRRPRLVAYFIMGFLVIAFILSVLGQVEIVAT---ANGKLTHSGRSKEIKP---- 101

Query: 61 PLSFRIAGKLVER-KVRLGDIVKKGQVVALLDTSDVARSAASAQAQLDAATHALTFAQQQ 119
I +V+ V+ G+ V+KG V+ L A+A +L A+ +
Sbjct: 102 -----IENSIVKEIIVKEGESVRKGDVLLKLTALG-------AEADTLKTQSSLLQARLE 149

Query: 120 RERDRA--QARENLIAPAQLEQTENAYASARAQRDQAAQQLA----------LAKNQLQY 167
+ R + ++ E P E + + + L + +L
Sbjct: 150 QTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNL 209

Query: 168 ATLVADHAGYITAEQADTGQ 187
A+ +
Sbjct: 210 DKKRAERLTVLARINRYENL 229



Score = 34.8 bits (80), Expect = 5e-04
Identities = 10/71 (14%), Positives = 27/71 (38%)

Query: 100 ASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYASARAQRDQAAQQLA 159
+ A+++ + + + + + + IA + + EN Y A + QL
Sbjct: 217 LTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLE 276

Query: 160 LAKNQLQYATL 170
++++ A
Sbjct: 277 QIESEILSAKE 287


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2163ACRIFLAVINRP433e-137 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 433 bits (1114), Expect = e-137
Identities = 223/1062 (20%), Positives = 423/1062 (39%), Gaps = 75/1062 (7%)

Query: 13 LSAWALRHQALVVYLIALATIAGILAYSRLAQSEDPPFTFRVMVIRTFWPGATARQVQEQ 72
++ + +R L + +AG LA +L ++ P + + +PGA A+ VQ+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 73 VTDRIGRKLQEMPAIDYLRSYS-RPGESMLFFAMKDSAPVKDVPQTWYQVRKKVGDISMT 131
VT I + + + + Y+ S S G + + QV+ K+ +
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQV---QVQNKLQLATPL 117

Query: 132 LPPGVQGP-FFNDEFGDVYTNIYTLEGDG--FSPAQLHDYAD-QLRVVLLRVPGVAKVDY 187
LP VQ ++ Y + D + + DY ++ L R+ GV V
Sbjct: 118 LPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQL 177

Query: 188 FGDPDQRIFVEIDNTRLARLGISPQQIAQAINAQNDVASPGVLTAAHD------RVFIRP 241
FG + + +D L + ++P + + QND + G L I
Sbjct: 178 FG-AQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIA 236

Query: 242 SGQYESVAAIADTLIRVN--GRTFRLGELATIKRGYDDPPVTQMRTIGRNANGRAVLGIG 299
++++ +RVN G RL ++A ++ G + + NG+ G+G
Sbjct: 237 QTRFKNPEEFGKVTLRVNSDGSVVRLKDVARVELG------GENYNVIARINGKPAAGLG 290

Query: 300 VTMQPGGDVIRLGKALDASAKALQAQLPAGLALTEVSSMPHAVARSVDDFLEAVAEAVAI 359
+ + G + + KA+ A LQ P G+ + V S+ + ++ + EA+ +
Sbjct: 291 IKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIML 350

Query: 360 VLIVSLVSLG-LRTGMVVVISIPVVLAVTALFMYLFDIGLHKVSLGTLVLALGLLVDDAI 418
V +V + L +R ++ I++PVVL T + F ++ +++ +VLA+GLLVDDAI
Sbjct: 351 VFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAI 410

Query: 419 IAVEMMA-VKLEQGFSRARAAAFAYTSTAFPMLTGTLVTVSGFLPIALAKSSTGEYTRSI 477
+ VE + V +E A + + ++ +V + F+P+A STG R
Sbjct: 411 VVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQF 470

Query: 478 FEVSAIALIASWFAAVVLIPLLGYHMLPERKHPRQDAAGAPHAP-DAAHDHAHGHDIYDT 536
A+ S A++L P L +L + G + DH+
Sbjct: 471 SITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHS-------V 523

Query: 537 RFYTRLRVWIKWCIERRFIVLAITIALFVVALAGFSLVPQQFFPSSDRPELLVDLRLPEG 596
YT + + L I + + F +P F P D+ L ++LP G
Sbjct: 524 NHYTNS---VGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAG 580

Query: 597 ASFNATLKEAERLEKLIAK--RPEIDHAVNFVGSGAPRFYLPLDQQLQLPNFAQFVITAK 654
A+ T K +++ K + ++ G Q N ++ K
Sbjct: 581 ATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSG---------QAQNAGMAFVSLK 631

Query: 655 SVDAR---EKLSAWLAPVLREQFPAARTRISRLENGPPV-------GYPVQ-FRVSGDSI 703
+ R E + + + + R N P + G+ + +G
Sbjct: 632 PWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGH 691

Query: 704 ATVRAIAEKVAATMR---ADARATNVQFDWDEPAERSVRFELDQHKARELNVSSQDVASF 760
+ ++ A + D + E+DQ KA+ L VS D+
Sbjct: 692 DALTQARNQLLGMAAQHPASLVSVRPNGLEDTA---QFKLEVDQEKAQALGVSLSDINQT 748

Query: 761 LAMTLSGTTLTQYRERDKLIAVDLRAPRAQRIDPASLAGLAMPTPNG-PVPLGSLGRFHD 819
++ L GT + + +R ++ + ++A R+ P + L + + NG VP + H
Sbjct: 749 ISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHW 808

Query: 820 TLEYGVVWERDRQPTITVQSDVTAGAQGIDVTHAIDAKLDALRAQLPVGYRIEIGGSVEE 879
+ + P++ +Q + G + A ++ L ++LP G + G +
Sbjct: 809 VYGSPRLERYNGLPSMEIQGEAAPG----TSSGDAMALMENLASKLPAGIGYDWTGMSYQ 864

Query: 880 STKGQTSINAQMPLMVIAVLTLLMIQLQSFSRVLMVVLTAPLGMIGVVGTLLLFGKPFGF 939
A + + + V L +S+S + V+L PLG++GV+ LF +
Sbjct: 865 ERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDV 924

Query: 940 VAMLGVIAMFGIIMRNSVILVDQIEQDIAA-GHGRFDAIVGATVRRFRPITLTAAAAVLA 998
M+G++ G+ +N++++V+ + + G G +A + A R RPI +T+ A +L
Sbjct: 925 YFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILG 984

Query: 999 LIPLLRSNFFG-----PMATALMGGITSATVLTLFFLPALYA 1035
++PL SN G + +MGG+ SAT+L +FF+P +
Sbjct: 985 VLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFV 1026



Score = 84.1 bits (208), Expect = 2e-18
Identities = 91/535 (17%), Positives = 182/535 (34%), Gaps = 67/535 (12%)

Query: 550 IERRFIVLAITIALFVVALAGFSLVPQQFFPSSDRPELLVDLRLPEGASFNATLKEAER- 608
I R + I L + +P +P+ P + V P A + +
Sbjct: 6 IRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYP-----GADAQTVQDT 60

Query: 609 ----LEKLIAKRPEIDH---AVNFVGSG--APRFYLPLDQQLQLPNFAQFVITAKSVDAR 659
+E+ + + + + GS F D P+ AQ V +
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTD-----PDIAQ-------VQVQ 108

Query: 660 EKLSAWLAPVLREQFP-AARTRISRLENGPPVGYPVQFRVSGDSIATVRAIAEKVAATMR 718
KL P + + +E V VS + T I++ VA+ ++
Sbjct: 109 NKLQL-----ATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVK 163

Query: 719 ADAR----ATNVQFDWDEPAERSVRFELDQHKARELNVSSQDVASFL--------AMTLS 766
+VQ A+ ++R LD + ++ DV + L A L
Sbjct: 164 DTLSRLNGVGDVQLF---GAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLG 220

Query: 767 GTTLTQYRERDKLIAVDLRAPRAQRIDPASLAGLAMPTPNG-PVPLGSLGRFHDTLE-YG 824
GT ++ + I R + +L +G V L + R E Y
Sbjct: 221 GTPALPGQQLNASIIAQTRFKNPEEFGKVTLRV----NSDGSVVRLKDVARVELGGENYN 276

Query: 825 VVWERDRQPTITVQSDVTAGAQGIDVTHAIDAKLDALRAQLPVGYRIEI----GGSVEES 880
V+ + +P + + GA +D AI AKL L+ P G ++ V+ S
Sbjct: 277 VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLS 336

Query: 881 TKGQTSINAQMPLMVIAVLTLLMIQLQSFSRVLMVVLTAPLGMIGVVGTLLLFGKPFGFV 940
+ +++ L + + LQ+ L+ + P+ ++G L FG +
Sbjct: 337 I--HEVVKTLFEAIMLVFLVMYLF-LQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTL 393

Query: 941 AMLGVIAMFGIIMRNSVILVDQIEQDIAAGHGRF-DAIVGATVRRFRPITLTAAAAVLAL 999
M G++ G+++ +++++V+ +E+ + +A + + + A
Sbjct: 394 TMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVF 453

Query: 1000 IPLL-----RSNFFGPMATALMGGITSATVLTLFFLPALYAAWFRVKPDERDPEP 1049
IP+ + + ++ + + ++ L PAL A + E
Sbjct: 454 IPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK 508


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2165LCRVANTIGEN310.005 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 31.2 bits (70), Expect = 0.005
Identities = 14/57 (24%), Positives = 25/57 (43%)

Query: 287 QRWLELFRHYAGDDPATQLKFREALANEPELMTGTWADDALLGFVREAMQHLAPARR 343
Q ++ + + P TQ + R +A +T DD +L + ++M H AR
Sbjct: 95 QNGIKRVKEFLESSPNTQWELRAFMAVMHFSLTADRIDDDILKVIVDSMNHHGDARS 151


72BURPS1106A_2468BURPS1106A_2473N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2468-39-0.653464hypothetical protein
BURPS1106A_2469-29-0.078252NfeD family protein
BURPS1106A_2470-29-0.251234phosphoenolpyruvate synthase
BURPS1106A_24710130.375169hypothetical protein
BURPS1106A_2472-1111.239818phytochelatin synthase
BURPS1106A_2473-1110.940146serine protease
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2468RTXTOXINA300.021 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 29.5 bits (66), Expect = 0.021
Identities = 18/92 (19%), Positives = 35/92 (38%), Gaps = 8/92 (8%)

Query: 221 INQAQGEAAAILAVAEANSQAIQKIAQAIQSQGGMDAVNLKVAEQYVGAFGNLAKAGNTL 280
INQ A++ + SQ + + + + ++ V K+ NL G L
Sbjct: 188 INQLVDTVASLNNNVNSFSQQLNTLGSVLSNTKHLNGVGNKLQN-----LPNLDNIGAGL 242

Query: 281 IVPSNLSDLSTAIASALTIVNRSAPGALAPGA 312
+S + +AI+++ + N A A
Sbjct: 243 DT---VSGILSAISASFILSNADADTRTKAAA 271


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2470PHPHTRNFRASE2654e-81 Phosphoenolpyruvate-protein phosphotransferase sign...
		>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase

signature.
Length = 572

Score = 265 bits (678), Expect = 4e-81
Identities = 99/441 (22%), Positives = 171/441 (38%), Gaps = 73/441 (16%)

Query: 383 IHDPSEMERVQPGDVLVADMTDPNWEPVMK-RASAIVTNRGGRTCHAAIIARELGVPAVV 441
+ S + ++ D+T + + K T+ GGRT H+AI++R L +PAVV
Sbjct: 145 VETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMSRSLEIPAVV 204

Query: 442 GCGDATDVLKDGALVTVSCAEGDEGKIYDGLLETEVSEVQRGE------------LPSVP 489
G + T+ ++ G +V V G EG + E EV + L P
Sbjct: 205 GTKEVTEKIQHGDMVIVD---GIEGIVIVNPTEEEVKAYEEKRAAFEKQKQEWAKLVGEP 261

Query: 490 --------VKIMMNVGNPQLAFDFSQLPNAGVGLARLEFIINNNIGVHPKAILEYPNVDA 541
V++ N+G P+ G+GL R EF+ + P
Sbjct: 262 STTKDGAHVELAANIGTPKDVDGVLANGGEGIGLYRTEFLYMDR-DQLPT---------- 310

Query: 542 DLKKAVESVARGHASPRAFYVDKLTEGIATIAAAFYPKPVIVRLSDFKSNEYKKLIGGSR 601
++ E + KPV++R D ++ +
Sbjct: 311 --------------------EEEQFEAYKEVVQRMDGKPVVIRTLDIGGDKELSYL---- 346

Query: 602 YEPDEENPMLGFRGASRYIAEDFAQAFEMECMALKRVRDEMGLTNVEIMVPFVRTVKQAE 661
P E NP LGFR + + F + AL R N+++M P + T+++
Sbjct: 347 QLPKELNPFLGFRAIRLCL--EKQDIFRTQLRALLRAS---TYGNLKVMFPMIATLEELR 401

Query: 662 RVVGLLGKFGLKRGDNG------LRLIMMCEVPSNAILAEEFLQHFDGFSIGSNDLTQLT 715
+ ++ + K G + + +M E+PS A+ A F + D FSIG+NDL Q T
Sbjct: 402 QAKAIMQEEKDKLLSEGVDVSDSIEVGIMVEIPSTAVAANLFAKEVDFFSIGTNDLIQYT 461

Query: 716 LGLDRDSGMELLAVDFDERDPAVKFMLKRAIDTCRKLDKYVGICGQGPSDHPDFAKWLAD 775
+ DR + E ++ + PA+ ++ I K+VG+CG+ D L
Sbjct: 462 MAADRMN--ERVSYLYQPYHPAILRLVDMVIKAAHSEGKWVGMCGEMAGD-EVAIPLLLG 518

Query: 776 EGIASISLNPDTVIETWQALA 796
G+ S++ +++ L
Sbjct: 519 LGLDEFSMSATSILPARSQLL 539


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2472cloacin354e-04 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 35.1 bits (80), Expect = 4e-04
Identities = 19/44 (43%), Positives = 22/44 (50%)

Query: 24 GVGVGVGVGVGVGVGVGGGGGGGGDGDGDGDGGGPNARQAASVY 67
G G G G+ G G G G GGG G G G G GG +A A +
Sbjct: 47 GGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAF 90



Score = 33.5 bits (76), Expect = 0.001
Identities = 24/69 (34%), Positives = 28/69 (40%), Gaps = 7/69 (10%)

Query: 14 GIGVGVGVGVGVGV-------GVGVGVGVGVGVGGGGGGGGDGDGDGDGGGPNARQAASV 66
G+GVG G G G G G G G+ G G G G GG G G G +A
Sbjct: 26 GLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVA 85

Query: 67 YRAAWHVPA 75
A+ PA
Sbjct: 86 APVAFGFPA 94


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2473SUBTILISIN417e-06 Subtilisin serine protease family (S8) signature.
		>SUBTILISIN#Subtilisin serine protease family (S8) signature.

Length = 326

Score = 41.4 bits (97), Expect = 7e-06
Identities = 21/122 (17%), Positives = 37/122 (30%), Gaps = 22/122 (18%)

Query: 337 VLYAAPSMLLSDITSAYNRAVVDNVAKVINVSLGVCEADARASGTQAADDRIFKSAVAQG 396
VL S I A+ + +I++SLG + + K AVA
Sbjct: 117 VLNKQGSGQYDWIIQGIYYAI-EQKVDIISMSLG------GPEDVPELHEAVKK-AVASQ 168

Query: 397 QTFVVAAGDAGAYECSVSRVSGGQGVPARSNYSVSEPATSPYVVAVGGTTLSTDRTTLAY 456
+ AAG+ G + + P V++VG + +
Sbjct: 169 ILVMCAAGNEGDGDDRTD--------------ELGYPGCYNEVISVGAINFDRHASEFSN 214

Query: 457 AG 458
+
Sbjct: 215 SN 216


73BURPS1106A_2513BURPS1106A_2520N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2513-191.361880DNA repair protein RadA
BURPS1106A_2514-172.813548alanine racemase
BURPS1106A_2515-172.478431lysophospholipid transporter LplT
BURPS1106A_2516-195.048741phosphomethylpyrimidine kinase
BURPS1106A_2517-2124.560978hypothetical protein
BURPS1106A_2518-394.433071hypothetical protein
BURPS1106A_2519-1122.535770uracil-DNA glycosylase
BURPS1106A_2520-2151.096566ribosomal-protein-alanine acetyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2513TCRTETOQM310.011 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 31.0 bits (70), Expect = 0.011
Identities = 16/79 (20%), Positives = 27/79 (34%), Gaps = 17/79 (21%)

Query: 104 LLQSLAQIASERPALYISGEESGAQIALRAQRLALLEGGASAADLKLLAEIQLEKIQATI 163
LL +L +I+ P L + + +I L L ++Q+E A +
Sbjct: 361 LLDALLEISDSDPLLRYYVDSATHEIILS-----------------FLGKVQMEVTCALL 403

Query: 164 DAERPDVAVIDSIQTIYSE 182
+ I IY E
Sbjct: 404 QEKYHVEIEIKEPTVIYME 422


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2514ALARACEMASE438e-156 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 438 bits (1127), Expect = e-156
Identities = 207/353 (58%), Positives = 270/353 (76%)

Query: 1 MPRPISATIHTAALANNLSVVRRHAAQSKVWAIVKANAYGHGLARVFPGLRGTDGFGLLD 60
M RPI A++ AL NLS+VR+ A ++VW++VKANAYGHG+ R++ + TDGF LL+
Sbjct: 1 MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLN 60

Query: 61 LDEAVKLRELGWAGPILLLEGFFRSTDIDVIDRYSLTTAVHNDEQMRMLETARLSKPVNV 120
L+EA+ LRE GW GPIL+LEGFF + D+++ D++ LTT VH++ Q++ L+ ARL P+++
Sbjct: 61 LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI 120

Query: 121 QLKMNSGMNRLGYTPEKYRAAWERARACPGIGQITLMTHFSDADGERGVAEQMATFERGA 180
LK+NSGMNRLG+ P++ W++ RA +G++TLM+HF++A+ G++ MA E+ A
Sbjct: 121 YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA 180

Query: 181 QGIAGARSFANSAAVLWHPSAHFDWVRPGIMLYGASPSGRAADIADRGLKPTMTLASELI 240
+G+ RS +NSAA LWHP AHFDWVRPGI+LYGASPSG+ DIA+ GL+P MTL+SE+I
Sbjct: 181 EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII 240

Query: 241 AVQTLAKGQAVGYGSMFVAEDTMRIGVVACGYADGYPRIAPEGTPVVVDGVRTRIVGRVS 300
VQTL G+ VGYG + A D RIG+VA GYADGYPR AP GTPV+VDGVRT VG VS
Sbjct: 241 GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS 300

Query: 301 MDMLTVDLTPVPQAGVGARVELWGETLPIDDVAARCMTVGYELMCAVAPRVPV 353
MDML VDLTP PQAG+G VELWG+ + IDDVAA TVGYELMCA+A RVPV
Sbjct: 301 MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPV 353


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2515TCRTETB290.040 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 29.1 bits (65), Expect = 0.040
Identities = 31/139 (22%), Positives = 54/139 (38%), Gaps = 4/139 (2%)

Query: 29 FFSSLADSALLIAAIALLKDLHAPNWMIPLLKLFFVLSYVVLAAFVGAFADSRPKGHVMF 88
FFS L + L ++ + D + P + F+L++ + A G +D ++
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 89 ITNSIKVVGCLIMLFGAHP----LIAYGIVGFGAAAYSPAKYGILTELLPPERLVAANGW 144
I G +I G ++A I G GAAA+ ++ +P E A G
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 145 IEGTTVGSIILGTVLGGAL 163
I +G +GG +
Sbjct: 144 IGSIVAMGEGVGPAIGGMI 162


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2519PYOCINKILLER290.043 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 29.0 bits (64), Expect = 0.043
Identities = 30/121 (24%), Positives = 39/121 (32%), Gaps = 13/121 (10%)

Query: 124 ARQAAAESGVRAAADAPAPAAAPESRTRDATIARGASPAEPDEPGVAGVVGVADEPSVAG 183
+ +AAA + R A A A A E + A I + A P V
Sbjct: 213 SIEAAAANKAREQAAAEAKRKAEEQARQQAAIRAANTYAMPANGSVV----------ATA 262

Query: 184 GARRARTSGGGAEVPASALDLTDAAEATRPAAAPAPAALTGGDAGAAASDEDMS-WFDLE 242
R GA A A ++DA A AP+ + G A S W D
Sbjct: 263 AGRGLIQVAQGAASLAQA--ISDAIAVLGRVLASAPSVMAVGFASLTYSSRTAEQWQDQT 320

Query: 243 P 243
P
Sbjct: 321 P 321


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2520SACTRNSFRASE443e-08 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 44.2 bits (104), Expect = 3e-08
Identities = 21/71 (29%), Positives = 32/71 (45%)

Query: 79 VAPVAQRSGVGLALLREAVRIARAERLDGVLLEVRPSNPRAIRLYERFGFVSVGRRRNYY 138
VA ++ GVG ALL +A+ A+ G++LE + N A Y + F+ Y
Sbjct: 97 VAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAVDTMLY 156

Query: 139 PAKHRSREDAI 149
+ E AI
Sbjct: 157 SNFPTANEIAI 167


74BURPS1106A_2583BURPS1106A_2591N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2583-2100.578218RND family efflux transporter MFP subunit
BURPS1106A_2584-310-0.038540AcrB/AcrD/AcrF family protein
BURPS1106A_2585-211-0.921676hypothetical protein
BURPS1106A_2586-214-1.974097GDSL-like lipase/acylhydrolase domain/outer
BURPS1106A_2587114-0.655966hypothetical protein
BURPS1106A_2588116-1.985255hypothetical protein
BURPS1106A_2589112-1.012134hypothetical protein
BURPS1106A_2591-110-1.121504*aspartate kinase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2583RTXTOXIND415e-06 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 41.4 bits (97), Expect = 5e-06
Identities = 19/150 (12%), Positives = 50/150 (33%), Gaps = 22/150 (14%)

Query: 50 AIVGTINLPVYLTGVGAVTPQYDVTVRSQVDGQITHVRFHEGQQVRAGDVLVEIDRRALQ 109
+++G + + G + + ++ + + + EG+ VR GDVL+++ +
Sbjct: 75 SVLGQVEIVATANGKLTHSGR-SKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAE 133

Query: 110 ATADQATAKLEQDKATLANARLEL----------------ARHQRLAEMNAAPVQML--- 150
A + + L Q + ++ Q ++E + L
Sbjct: 134 ADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKE 193

Query: 151 --DTWKARVNELHAQIRGDQAAVQNARVAV 178
TW+ + + + +A +
Sbjct: 194 QFSTWQNQKYQKELNLDKKRAERLTVLARI 223



Score = 29.0 bits (65), Expect = 0.040
Identities = 14/94 (14%), Positives = 39/94 (41%), Gaps = 11/94 (11%)

Query: 100 LVEIDRRALQATADQAT--AKLEQDKATLANARLELARHQRLAEMNAAPVQMLDTWKARV 157
++E + + ++A + ++LEQ ++ + +A+ E +L + + ++
Sbjct: 254 VLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFK---------NEILDKL 304

Query: 158 NELHAQIRGDQAAVQNARVAVDYTTIRAPISGRI 191
+ I + + IRAP+S ++
Sbjct: 305 RQTTDNIGLLTLELAKNEERQQASVIRAPVSVKV 338


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2584ACRIFLAVINRP7550.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 755 bits (1952), Expect = 0.0
Identities = 273/1033 (26%), Positives = 496/1033 (48%), Gaps = 26/1033 (2%)

Query: 9 FIRYPVATCLMTAGILFAGVAAYFHLPVAPLPQVEFPTIQVSAVLPGADPVSVASTLAQP 68
FIR P+ ++ ++ AG A LPVA P + P + VSA PGAD +V T+ Q
Sbjct: 5 FIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVTQV 64

Query: 69 LETQFSKIPYVTQMTSQSTLS-STSIVLQFSLERSIDAAANDVQSAIDAAAAQLPADLPS 127
+E + I + M+S S + S +I L F D A VQ+ + A LP ++
Sbjct: 65 IEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQ 124

Query: 128 PPTFQKVNPADSPIMLLSAISSTLPLTTID--DYVETRLTKSLSQIDGVGSVSIGGQQKP 185
+ S +M+ +S T D DYV + + +LS+++GVG V + G Q
Sbjct: 125 QGIS-VEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQY- 182

Query: 186 SIRIQLDPVKLASRGLSSEDVRHALSGLSGVNPKGVFNGT------TRSYTIYTNGQLTE 239
++RI LD L L+ DV + L + G GT + +I +
Sbjct: 183 AMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFKN 242

Query: 240 PAQWNDAIV-AYRDGTPVRIRDIGQAVLGPEDNTLAAWIDGRRAISVGIYKKPGANTVST 298
P ++ + DG+ VR++D+ + LG E+ + A I+G+ A +GI GAN + T
Sbjct: 243 PEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDT 302

Query: 299 VDKILARLPELEASLPPSLKIAVLADRTQTIRASLLDIELTLLLNVVLVVVVIYAFLGSV 358
I A+L EL+ P +K+ D T ++ S+ ++ TL ++LV +V+Y FL ++
Sbjct: 303 AKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNM 362

Query: 359 RTTIIPAVTVPVSLFGACALMWVCGYSLDNISLMAMTIAVGFVVDDAIVMVENIARH-VE 417
R T+IP + VPV L G A++ GYS++ +++ M +A+G +VDDAIV+VEN+ R +E
Sbjct: 363 RATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMME 422

Query: 418 AGERPLQAALKGLSETSFTIASISLSLVAVLLPLLLMSGIIGRMFREFAVTLSMTIIVSA 477
P +A K +S+ + I++ L AV +P+ G G ++R+F++T+ + +S
Sbjct: 423 DKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSV 482

Query: 478 FVSLTLTPMMASYLLRAHRHDAGRPPRP--GLFERAFARTAAAYERALDVALRHRFVTLC 535
V+L LTP + + LL+ + G F F + Y ++ L L
Sbjct: 483 LVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLL 542

Query: 536 AFFASVAASVFLYVGIPKGFFPQQDTGVITGISEAAQTISVEDMARHSMALAAIIRADPA 595
+ VA V L++ +P F P++D GV + + + E + + +
Sbjct: 543 IYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEK 602

Query: 596 --VEHCQMAVGGSAYAGTTVNNGRWYITLKPRDQRDA---TADEVIRRLRPQFAKVPGVR 650
VE V G +++G N G +++LKP ++R+ +A+ VI R + + K+
Sbjct: 603 ANVES-VFTVNGFSFSGQAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGF 661

Query: 651 MYLQAAQDVIIGARLARTQYQLTLQSA-DVGALTTWAPRLLARLSGLP-QLRDVASDQQV 708
+ ++ ++L Q+ ALT +LL + P L V +
Sbjct: 662 VIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLE 721

Query: 709 NGSALSVAIDRDQAARYGLTPEAIDGTLYDAFGSRQVAQYFTQLSTYKVIMETLPSLQRD 768
+ + + +D+++A G++ I+ T+ A G V + + K+ ++ +
Sbjct: 722 DTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRML 781

Query: 769 PGTLDRIYMKAPSGALVPLSSVARWTTDTVQPLSVNHQSHFPSVTISFNLAPGVSLGEAT 828
P +D++Y+++ +G +VP S+ + + + PS+ I APG S G+A
Sbjct: 782 PEDVDKLYVRSANGEMVPFSAFTT-SHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAM 840

Query: 829 AAIEAARASLRMPPAVVGSFQGTAQAFQSTLATMPMLILSALIVAYLVLGALYGSFIHPW 888
A +E + ++P + + G + + + P L+ + +V +L L ALY S+ P
Sbjct: 841 ALME--NLASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPV 898

Query: 889 TILSTLPSAGVGAIATLWLFKYDFNLIALIGVILLIGIVKKNGIMMVDFAIAATRERNMT 948
+++ +P VG + LF ++ ++G++ IG+ KN I++V+FA +
Sbjct: 899 SVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKG 958

Query: 949 SLDAIRSACLLRLRPIMMTTMTALFGALPLMLTPGMGSELRQPLGYAMVGGLLVSQVLTL 1008
++A A +RLRPI+MT++ + G LPL ++ G GS + +G ++GG++ + +L +
Sbjct: 959 VVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAI 1018

Query: 1009 FTTPVIYLYLDTL 1021
F PV ++ +
Sbjct: 1019 FFVPVFFVVIRRC 1031



Score = 90.3 bits (224), Expect = 2e-20
Identities = 78/509 (15%), Positives = 163/509 (32%), Gaps = 37/509 (7%)

Query: 4 NLFAVFIRYPVATCLMTAGILFAGVAAYFHLPVAPLPQVEFPTIQVSAVLP-GADPVSVA 62
N + L+ A I+ V + LP + LP+ + LP GA
Sbjct: 528 NSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQ 587

Query: 63 STLAQPLETQF---SKIPYVTQMTSQSTLSSTS-------IVLQFSLERSIDAAANDVQS 112
L Q + + + S + + L+ ER + N ++
Sbjct: 588 KVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAGMAFVSLKPWEER--NGDENSAEA 645

Query: 113 AIDAAAAQLPADLPSPPTFQKVNPADSPIMLLSAIS---------STLPLTTIDDYVETR 163
I A + L + I+ L + + L +
Sbjct: 646 VIHRAKME----LGKIRDGFVIPFNMPAIVELGTATGFDFELIDQAGLGHDALTQARNQL 701

Query: 164 LTKSLSQIDGVGSVSIGGQQ-KPSIRIQLDPVKLASRGLSSEDVRHALSGLSGVNPKGVF 222
L + + SV G + ++++D K + G+S D+ +S G F
Sbjct: 702 LGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDF 761

Query: 223 NGTTRSYTIYTNGQ---LTEPAQWNDAIVAYRDGTPVRIRDIGQAVLGPEDNTLAAWIDG 279
R +Y P + V +G V + L +G
Sbjct: 762 IDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVPFSAFTTSHWVYGSPRLER-YNG 820

Query: 280 RRAISVGIYKKPGANTVSTVDKILARLPELEASLPPSLKIAVLADRTQTIRASLLDIELT 339
++ + PG ++ +A + L + LP + + R S
Sbjct: 821 LPSMEIQGEAAPG----TSSGDAMALMENLASKLPAGIGYDW-TGMSYQERLSGNQAPAL 875

Query: 340 LLLNVVLVVVVIYAFLGSVRTTIIPAVTVPVSLFGACALMWVCGYSLDNISLMAMTIAVG 399
+ ++ V+V + + A S + + VP+ + G + D ++ + +G
Sbjct: 876 VAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIG 935

Query: 400 FVVDDAIVMVENI-ARHVEAGERPLQAALKGLSETSFTIASISLSLVAVLLPLLLMSGII 458
+AI++VE + G+ ++A L + I SL+ + +LPL + +G
Sbjct: 936 LSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAG 995

Query: 459 GRMFREFAVTLSMTIIVSAFVSLTLTPMM 487
+ + ++ + +++ P+
Sbjct: 996 SGAQNAVGIGVMGGMVSATLLAIFFVPVF 1024


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2586IGASERPTASE300.026 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 30.4 bits (68), Expect = 0.026
Identities = 34/191 (17%), Positives = 52/191 (27%), Gaps = 22/191 (11%)

Query: 335 SDRLSLFADVGYTRNFHG--AAGGMNAFDSDVEMFSIGADYKLSEASRAGALLSSGNANG 392
S+ + L Y RN + A N + + Y G L G
Sbjct: 1331 SNNVQLGGVFTYVRNSNNFDKATSKNTL----AQVNFYSKYYADNHWYLGIDLGYGKFQS 1386

Query: 393 SLAGGQGR-IGLHAYRLGVY--HAFERAGLFVRAYAGAGWSR-----YRL--DRAAVLPG 442
L H + G+ AF + G +S + L R V P
Sbjct: 1387 KLQTNHNAKFARHTAQFGLTAGKAFNLGNFGITPIVGVRYSYLSNADFALDQARIKVNPI 1446

Query: 443 AVRASTSGFDFGALVKAGYLFALGGVRLGPVADVGYTQLVARGYTEDGDPILAQNVGVQR 502
+V+ + + D Y + LG + P+ Y G A NV Q+
Sbjct: 1447 SVKTAFAQVDLS------YTYHLGEFSVTPILSARYDANQGSGKINVNGYDFAYNVENQQ 1500

Query: 503 LKGVSAGAGVR 513

Sbjct: 1501 QYNAGLKLKYH 1511


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2591CARBMTKINASE362e-04 Bacterial carbamate kinase signature.
		>CARBMTKINASE#Bacterial carbamate kinase signature.

Length = 314

Score = 36.0 bits (83), Expect = 2e-04
Identities = 33/119 (27%), Positives = 56/119 (47%), Gaps = 15/119 (12%)

Query: 116 IDDERVRRDLDAGKVVIITGFQGV---DPDGHITTL-GRGGSDTSAVAVAAALEADECLI 171
++ E +++ ++ G +VI +G GV DG I + D + +A + AD +I
Sbjct: 174 VEAETIKKLVERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMI 233

Query: 172 YTDVDGVYTTDPRVVEEARRLDSVTFEEMLEMA--------SLGSKVLQ-IRSVEFAGK 221
TDV+G E+ + L V EE+ + S+G KVL IR +E+ G+
Sbjct: 234 LTDVNGAALYYGT--EKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWGGE 290


75BURPS1106A_2663BURPS1106A_2670N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_2663-116-1.818443D-alanyl-D-alanine carboxypeptidase
BURPS1106A_2664014-1.896967phasin family protein
BURPS1106A_2665013-1.863479pyruvate dehydrogenase complex E3 component,
BURPS1106A_2666012-1.478796dihydrolipoamide acetyltransferase
BURPS1106A_2667012-1.911953pyruvate dehydrogenase subunit E1
BURPS1106A_2668011-1.109724hypothetical protein
BURPS1106A_2669010-0.520299sensory box histidine kinase
BURPS1106A_26701120.678572LuxR family DNA-binding response regulator
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2663SSBTLNINHBTR280.027 Streptomyces subtilisin inhibitor signature.
		>SSBTLNINHBTR#Streptomyces subtilisin inhibitor signature.

Length = 144

Score = 28.3 bits (62), Expect = 0.027
Identities = 15/50 (30%), Positives = 23/50 (46%)

Query: 25 VATAAVAPADAFAATAKTAQSAKGKKSAAKKSLRAASSSAEPRAKGARKR 74
+A+ A APA +A +A G+ +A LRA + + P A G
Sbjct: 27 LASPATAPASLYAPSALVLTVGHGESAATAAPLRAVTLTCAPTASGTHPA 76


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2666RTXTOXIND365e-04 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 35.6 bits (82), Expect = 5e-04
Identities = 12/58 (20%), Positives = 22/58 (37%)

Query: 49 VPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAA 106
+ VKE+ VK G++V +G +++ L A A + + A
Sbjct: 99 IKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQIL 156



Score = 31.0 bits (70), Expect = 0.014
Identities = 12/37 (32%), Positives = 21/37 (56%)

Query: 162 VPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAA 198
+ +VK+I VK G++V +G +++ L A G A
Sbjct: 99 IKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEAD 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2669PF06580320.012 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 31.8 bits (72), Expect = 0.012
Identities = 18/85 (21%), Positives = 31/85 (36%), Gaps = 18/85 (21%)

Query: 700 PVLIEQVLV-NLMKNAAEAMQEARPQAENGVIRVVADLEAGFVDIRVIDQGPGVDEATAE 758
P ++ Q LV N +K+ + G I + + G V + V + G + T E
Sbjct: 256 PPMLVQTLVENGIKHGIA------QLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE 309

Query: 759 RLFEPFYSTKSDGMGMGLNICRSII 783
S G G+ N+ +
Sbjct: 310 ----------STGTGL-QNVRERLQ 323


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_2670HTHFIS1132e-31 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 113 bits (283), Expect = 2e-31
Identities = 39/153 (25%), Positives = 67/153 (43%), Gaps = 4/153 (2%)

Query: 11 TVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLDAYQPAQQAGQIACLILDVRMSGM 70
T+ V DDD A+R L L GY V+ S+A AG ++ DV M
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIA----AGDGDLVVTDVVMPDE 60

Query: 71 SGLELQERLIAENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLE 130
+ +L R+ LP++ ++ A+ +KGA D++ KPFD EL ++ R L
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 131 KARNESKSVQEQRAASERLSKLTAREQQVLERI 163
+ + +++ L +A Q++ +
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVL 153


76BURPS1106A_3134BURPS1106A_3141N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3134541-9.576148NAD-dependent epimerase/dehydratase family
BURPS1106A_3135437-9.250435O-antigen acetylase WbiA
BURPS1106A_3136229-7.556034lipopolysaccharide ABC transporter ATP-binding
BURPS1106A_3137123-5.483098lipopolysaccharide ABC transporter permease
BURPS1106A_3138-116-2.879921dTDP-4-dehydrorhamnose reductase
BURPS1106A_3139-310-1.049607dTDP-4-dehydrorhamnose 3,5-epimerase
BURPS1106A_3140-211-0.223379glucose-1-phosphate thymidylyltransferase
BURPS1106A_3141-3101.070375dTDP-glucose 4,6-dehydratase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3134NUCEPIMERASE1682e-51 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 168 bits (426), Expect = 2e-51
Identities = 83/363 (22%), Positives = 136/363 (37%), Gaps = 58/363 (15%)

Query: 6 KILVTGGAGFIGCAISERLAARASRYVVMDNLHPQIHASAVRPGALHEKAE----LVVAD 61
K LVTG AGFIG +S+RL + V +DNL+ + +++ L A+ D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDY-YDVSLKQARLELLAQPGFQFHKID 60

Query: 62 VTDAGAWDALLSDFQPEIIIHLAAETGTGQSLTEASRHALVNVVGTTRLTDALVKHGIVV 121
+ D L + E + SL +A N+ G + + + +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK--I 118

Query: 122 EHILLTSSRAVYGEGAWQKDDGTIVYPGQRGRAQLEAAQWDFPGMTMLPSRADRTEPRPT 181
+H+L SS +VYG +P D + P
Sbjct: 119 QHLLYASSSSVYGLN------------------------------RKMPFSTDDSVDHPV 148

Query: 182 SVYGATKLAQEHVLRAWSLATKTPLSILRLQNVYGPGQSLTNSYTGIVALFSRLAREKKV 241
S+Y ATK A E + +S P + LR VYGP + F++ E K
Sbjct: 149 SLYAATKKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMALF----KFTKAMLEGKS 204

Query: 242 IPLYEDGNVTRDFVSIDDVADAIVATLVRTPEA-----------------LSLFDIGSGQ 284
I +Y G + RDF IDD+A+AI+ P A +++IG+
Sbjct: 205 IDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSS 264

Query: 285 ATSILDMARIIAAHYGAPEPQINGAFRDGDVRHAACDLSESLANLGWKPQWSLKRGIGEL 344
++D + + G + + GDV + D +G+ P+ ++K G+
Sbjct: 265 PVELMDYIQALEDALGIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNF 324

Query: 345 QTW 347
W
Sbjct: 325 VNW 327


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3137ABC2TRNSPORT300.007 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 30.3 bits (68), Expect = 0.007
Identities = 16/59 (27%), Positives = 24/59 (40%)

Query: 195 LFTMVLMFLSPVFYPASALPEKYRFWLELNPLTLFIEQSRGILLEGRVPDFHPLGLAFL 253
L ++FLS +P LP ++ PL+ I+ R I+L V D A
Sbjct: 184 LVITPILFLSGAVFPVDQLPIVFQTAARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALC 242


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3138NUCEPIMERASE587e-12 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 58.3 bits (141), Expect = 7e-12
Identities = 32/160 (20%), Positives = 56/160 (35%), Gaps = 27/160 (16%)

Query: 1 MKILVTGANGQVGWELARSLAVLGQVV-----------PLTRE--------------QAD 35
MK LVTGA G +G+ +++ L G V ++ + D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 36 LGRPETLARIVEDAKPDVVVNAAAYTAVDAAETDGAAANVINGEA-VGVLAAATKRVGGL 94
L E + + + V + AV + + A N + +L
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 95 FVHYSTDYVFDGTKPSPYIETDPT-CPVNAYGASKLLGEL 133
++ S+ V+ + P+ D PV+ Y A+K EL
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL 160


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3141NUCEPIMERASE1747e-54 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 174 bits (443), Expect = 7e-54
Identities = 90/350 (25%), Positives = 136/350 (38%), Gaps = 45/350 (12%)

Query: 2 ILVTGGAGFIGANFVLDWLAQSDEAVLNVDKLT--YAGNLGTLK-SLQGNPKHVFARVDI 58
LVTG AGFIG + L + V+ +D L Y +L + L P F ++D+
Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQ-VVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61

Query: 59 CDRAAIDALLAQHKPRAIVHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWSALG 118
DR + L A + V S+ P + +N+ G +LE R
Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN----- 116

Query: 119 TDAKAAFRFLHVSTDEVFGSLSPADPQFSETTPYA-PNSPYSATKAGSDHLVRAYHHTYG 177
L+ S+ V+G L+ P FS P S Y+ATK ++ + Y H YG
Sbjct: 117 ----KIQHLLYASSSSVYG-LNRKMP-FSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170

Query: 178 LPVLTTNCSNNYGPYQFPEKLIPLMIANALGGKPLPVYGDGQNVRDWLYVGDHCSAIREV 237
LP YGP+ P+ + L GK + VY G+ RD+ Y+ D AI +
Sbjct: 171 LPATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRL 230

Query: 238 L------------------ARGVPGETYNVGGWNEKKNLDVVHTLCDLLD-EARPKAGGS 278
A P YN+G + + +D + L D L EA+
Sbjct: 231 QDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKN---- 286

Query: 279 YRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLAKTVRWYLD 328
+ +PG + D + L +G+ P T + G+ V WY D
Sbjct: 287 ------MLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRD 330


77BURPS1106A_3728BURPS1106A_3732N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3728011-1.510646ABC-2 type transporter, permease
BURPS1106A_3729-111-1.199242ABC transporter ATP-binding protein
BURPS1106A_3730-112-0.892344hypothetical protein
BURPS1106A_3731-3110.411587toluene tolerance protein
BURPS1106A_3732-4111.125552VacJ family lipoprotein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3728ABC2TRNSPORT741e-17 ABC-2 type transport system membrane protein signat...
		>ABC2TRNSPORT#ABC-2 type transport system membrane protein

signature.
Length = 262

Score = 73.8 bits (181), Expect = 1e-17
Identities = 60/243 (24%), Positives = 100/243 (41%), Gaps = 6/243 (2%)

Query: 7 LFYKEILRFWKVSFQTVLAPVVTALLYLTIFGHALTGRVNVYPGVEYVSFLVPGLVMMSV 66
++ + + + K + ++L + L+YL G L V GV Y +FL G+V S
Sbjct: 19 VWRRNYIAWKKAALASLLGHLAEPLIYLFGLGAGLGVMVGRVGGVSYTAFLAAGMVATSA 78

Query: 67 LQNA-FANSSSSLIQSKITGNLVFMLLPPLSSADIFGAYVLASVVRGLAVGAGVFVVTVW 125
+ A F ++ + + ML L DI + + + GAG+ VV
Sbjct: 79 MTAATFETIYAAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALAGAGIGVVAAA 138

Query: 126 FIPMSFAAPLYIVAFALFGSAILGTLGLIAGIWAEKFDQLAAFQNFLIMPLTFLSGVFYS 185
+ + LY + +LG++ A +D +Q +I P+ FLSG +
Sbjct: 139 LGYTQWLSLLYALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFP 198

Query: 186 THSLPPVWREVSRLNPFFYMIDGFRYGFFG--IADVNPLASLS---VVAGFFVLLALIAM 240
LP V++ +R P + ID R G + DV +V FF+ AL+
Sbjct: 199 VDQLPIVFQTAARFLPLSHSIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTALLRR 258

Query: 241 RLL 243
RLL
Sbjct: 259 RLL 261


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3729PF05272280.037 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 28.5 bits (63), Expect = 0.037
Identities = 11/19 (57%), Positives = 13/19 (68%)

Query: 34 LLGPNGAGKTTLISILAGL 52
L G G GK+TLI+ L GL
Sbjct: 601 LEGTGGIGKSTLINTLVGL 619


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3731FLGMOTORFLIG280.026 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 28.2 bits (63), Expect = 0.026
Identities = 12/73 (16%), Positives = 22/73 (30%)

Query: 74 RTTQLAMGRNWRTATPAQQQQVIEQFKQLLIRTYSGALAQLKPDQQIQYPPFRADADATD 133
R + A ++ +Q +T + L+ L P + T+
Sbjct: 107 NLGSALQSRPFEFVRRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTN 166

Query: 134 VVVRTVAMNNGQP 146
V R M+ P
Sbjct: 167 VARRIALMDRTSP 179


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3732VACJLIPOPROT2242e-74 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 224 bits (572), Expect = 2e-74
Identities = 85/220 (38%), Positives = 114/220 (51%), Gaps = 8/220 (3%)

Query: 32 AAAALSGCATVQTPTKG--DPFEGFNRTMYTFNDKV-DQYALKPVARGYQWAVPQPMRDS 88
L GCA+ T +G DP EGFNRTMY FN V D Y ++PVA ++ VPQP R+
Sbjct: 11 GTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNG 70

Query: 89 VTNFFSNIGDVYIAANNLVQLKIADGVGDIMRVVINTVFGVGGLFDVATLAKLPKHAND- 147
++NF N+ + + N +Q G+ R +NT+ G+GG DVA +A +
Sbjct: 71 LSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEP 130

Query: 148 --FGVTLGHYGVPSGPYLVLPLLGPSTVRDTAGLAVDYAGNPLTYVRPDGVSWGLFGLNL 205
FG TLGHYGV GPY+ LP G T+RD G D A P+ +S G + L
Sbjct: 131 HRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMAD-ALYPVLSWLTWPMSVGKWTLEG 189

Query: 206 VNTRANLLGAGDVLEAAAIDKYSFVRNAYLQRRQALIGGA 245
+ TRA LL + +L + D Y VR AY QR + G
Sbjct: 190 IETRAQLLDSDGLLR-QSSDPYIMVREAYFQRHDFIANGG 228


78BURPS1106A_3925BURPS1106A_3938N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
BURPS1106A_3925-290.272630flagellar biosynthesis protein FlhB
BURPS1106A_3926-2110.1199903-demethylubiquinone-9 3-methyltransferase
BURPS1106A_3927-2110.298705lipoprotein
BURPS1106A_3928-313-0.139729hypothetical protein
BURPS1106A_3929-1120.700225chemotaxis regulator CheZ
BURPS1106A_3930-1110.406336chemotaxis protein CheY
BURPS1106A_39310121.410178chemotaxis-specific methylesterase
BURPS1106A_39321121.420542chemoreceptor glutamine deamidase CheD
BURPS1106A_39332121.174659chemotaxis protein CheR
BURPS1106A_39342130.927799methyl-accepting chemotaxis protein
BURPS1106A_3935213-0.496748chemotaxis protein CheW
BURPS1106A_3936213-0.324789chemotaxis protein CheA
BURPS1106A_3937012-2.535012chemotaxis protein CheY
BURPS1106A_3938013-2.650561flagellar motor protein MotB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3925TYPE3IMSPROT359e-125 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 359 bits (922), Expect = e-125
Identities = 108/344 (31%), Positives = 181/344 (52%), Gaps = 2/344 (0%)

Query: 8 DRTEAATPKRREKAREEGQVARSRELASFALLSAGFYGAWMLSGPIGEHLRTMLHAAFSF 67
++TE TPK+ AR++GQVA+S+E+ S AL+ A LS EH ++
Sbjct: 4 EKTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLM--LIPA 61

Query: 68 DRAAAFDTNRMLSHAGTLSLEGLYALAPVLALTGVAALAAPMAMGGWLVSTKTFELKFER 127
+++ + + + LE Y P+L + + A+A+ + G+L+S + + ++
Sbjct: 62 EQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKK 121

Query: 128 LNPITGLGRIFSIQGPIQLGMSIAKTLVVGGIGGIAIWRSKDELLGLATQPLHAALADAL 187
+NPI G RIFSI+ ++ SI K +++ + I I + LL L T +
Sbjct: 122 INPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLG 181

Query: 188 HLVAVCCGMTVAGMLVVAGLDVPYQLWQYNKKLRMTKEEVKREHRENEGDPHVKGRIRQQ 247
++ + G +V++ D ++ +QY K+L+M+K+E+KRE++E EG P +K + RQ
Sbjct: 182 QILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQF 241

Query: 248 QRAMARRRMMANVPTADVVVTNPTHFAVALKYTDGEMRAPKVVAKGVNLVAARIRELAAE 307
+ + R M NV + VVV NPTH A+ + Y GE P V K + +R++A E
Sbjct: 242 HQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEE 301

Query: 308 HHVPLLEAPPLARALYHNVELEREIPGTLYSAVAEVLAWVYQLK 351
VP+L+ PLARALY + ++ IP A AEVL W+ +
Sbjct: 302 EGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQN 345


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3927cloacin320.004 Cloacin signature.
		>cloacin#Cloacin signature.

Length = 551

Score = 32.0 bits (72), Expect = 0.004
Identities = 14/43 (32%), Positives = 20/43 (46%)

Query: 28 GGGGDGGSNASVNTGTGGGDTSAGGGSNGGTGGTGGSGSTPLA 70
GGG G + +G G G + G GTGG + + P+A
Sbjct: 47 GGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVA 89



Score = 30.1 bits (67), Expect = 0.022
Identities = 17/56 (30%), Positives = 22/56 (39%)

Query: 17 AAATAALVAACGGGGDGGSNASVNTGTGGGDTSAGGGSNGGTGGTGGSGSTPLASN 72
A +T+ + G G AS +G + GGGS G GGSG N
Sbjct: 13 AHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN 68



Score = 29.7 bits (66), Expect = 0.025
Identities = 21/61 (34%), Positives = 27/61 (44%), Gaps = 7/61 (11%)

Query: 28 GGGGDGGSNASVNTGTGGGDTS-------AGGGSNGGTGGTGGSGSTPLASNQAAITVST 80
GG DG +S N GGG S +G G+ GG G +GG T + A V+
Sbjct: 31 GGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAF 90

Query: 81 G 81
G
Sbjct: 91 G 91


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3930HTHFIS865e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 86.4 bits (214), Expect = 5e-23
Identities = 32/110 (29%), Positives = 52/110 (47%), Gaps = 4/110 (3%)

Query: 1 MDKSMKILVVDDFPTMRRIVRNLLKELGYSNVDEAEDGLAGLARLRGGGYDFVISDWNMP 60
M + ILV DD +R ++ L GY V + + G D V++D MP
Sbjct: 1 MTGA-TILVADDDAAIRTVLNQALSRAGYD-VRITSNAATLWRWIAAGDGDLVVTDVVMP 58

Query: 61 NLDGLAMLKEIRADASLTHLPVLMVTAESKKENIIAAAQAGASGYVVKPF 110
+ + +L I+ LPVL+++A++ I A++ GA Y+ KPF
Sbjct: 59 DENAFDLLPRIKKARP--DLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3931HTHFIS664e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.4 bits (162), Expect = 4e-14
Identities = 32/146 (21%), Positives = 62/146 (42%), Gaps = 14/146 (9%)

Query: 1 MQKKIKVLCVDDSALIRSLMTEIINSQPDMEVCATAPDPLVARELIKQHNPDVLTLDVEM 60
M +L DD A IR+++ + ++ + I + D++ DV M
Sbjct: 1 MTG-ATILVADDDAAIRTVLNQALSRAG--YDVRITSNAATLWRWIAAGDGDLVVTDVVM 57

Query: 61 PRMDGLDFLEKLMRLRP-MPVVMVSSLTERGSEITLRALELGAVDFVTKPRVGIRDGMLD 119
P + D L ++ + RP +PV+++S+ ++A E GA D++ KP D
Sbjct: 58 PDENAFDLLPRIKKARPDLPVLVMSAQNT--FMTAIKASEKGAYDYLPKP--------FD 107

Query: 120 YSEKLADKVRAASRARVRQNPQPHAA 145
+E + RA + + R + +
Sbjct: 108 LTELIGIIGRALAEPKRRPSKLEDDS 133


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3936PF06580463e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 46.0 bits (109), Expect = 3e-07
Identities = 21/151 (13%), Positives = 50/151 (33%), Gaps = 52/151 (34%)

Query: 467 ELDKSLIERIIDPLT--HLVRNSLDHGIETVEARRAAGKDAVGQLVLSAAHHGGNIVIEV 524
+++ ++++ + P+ LV N + HGI G+++L G + +EV
Sbjct: 245 QINPAIMDVQVPPMLVQTLVENGIKHGIA--------QLPQGGKILLKGTKDNGTVTLEV 296

Query: 525 SDDGAGLNRERILAKAAKQGMQISENISDDEVWNLIFAPGFSTAEVVTDVSGRGVGMDVV 584
+ G+ + G G+ V
Sbjct: 297 ENTGSLALKNTK--------------------------------------ESTGTGLQNV 318

Query: 585 KRNIQSMGG---HVEISSQAGRGTTTRIVLP 612
+ +Q + G +++S + G +++P
Sbjct: 319 RERLQMLYGTEAQIKLSEKQG-KVNAMVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3937HTHFIS718e-18 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 71.4 bits (175), Expect = 8e-18
Identities = 38/114 (33%), Positives = 58/114 (50%), Gaps = 2/114 (1%)

Query: 4 TILAIDDSATMRTLLSATLGEAGYDVTVASDGEVGLDVALATRFDLVLTDHHMPRKNGLE 63
TIL DD A +RT+L+ L AGYDV + S+ A DLV+TD MP +N +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 64 LIVALRRQLGYEATPILVLTTENGDAFKDAARAAGATGWIEKPIDPDALIELVA 117
L+ +++ P+LV++ +N A GA ++ KP D LI ++
Sbjct: 65 LLPRIKKA--RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
BURPS1106A_3938OMPADOMAIN401e-05 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 39.5 bits (92), Expect = 1e-05
Identities = 25/117 (21%), Positives = 51/117 (43%), Gaps = 9/117 (7%)

Query: 182 FAMSSDAVEPYMRDILREIGKTLNDV---PNRIIVQGHTDAVPYAGGEKGYSNWELSADR 238
F + ++P + L ++ L+++ ++V G+TD + G Y N LS R
Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRI----GSDAY-NQGLSERR 277

Query: 239 ANASRRELIAGGMDEAKVLRV-LGLASTQNLNKADPLDPENRRISIIVLNRKSELAL 294
A + LI+ G+ K+ +G ++ N D + I + +R+ E+ +
Sbjct: 278 AQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334



 
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