PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
Genome2117.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in NC_009900 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1RMA_0113RMA_0138Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0113213-1.117969OmpW family outer-membrane protein
RMA_0114211-1.061684S-adenosylmethionine transporter
RMA_0115310-1.064957proline/betaine transporter
RMA_0116311-0.798346hypothetical protein
RMA_011719-0.522156*preprotein translocase subunit SecG
RMA_0119010-0.565699cell surface antigen Sca2
RMA_0120-180.915917cysteinyl-tRNA synthetase
RMA_0121090.73788130S ribosomal protein S2
RMA_01222100.646582elongation factor Ts
RMA_0125110-1.8125763-deoxy-D-manno-octulosonic-acid transferase
RMA_0126311-2.418590hypothetical protein
RMA_0127210-2.203619aspartate aminotransferase
RMA_0128412-3.134821hypothetical protein
RMA_0129212-1.974199antitoxin of toxin-antitoxin
RMA_0130312-0.959645hypothetical protein
RMA_01312140.578872VacJ lipoprotein precursor
RMA_01322140.101693ABC transporter
RMA_01331150.398463ribose-phosphate pyrophosphokinase
RMA_01341130.431574alanine racemase
RMA_01350120.252964ABC transporter permease
RMA_0136-115-1.122749ribonucleotide ABC transporter ATP-binding
RMA_0137214-1.342406hypothetical protein
RMA_0138214-1.875816hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0115TCRTETA340.001 Tetracycline resistance protein signature.
		>TCRTETA#Tetracycline resistance protein signature.

Length = 399

Score = 33.6 bits (77), Expect = 0.001
Identities = 69/332 (20%), Positives = 120/332 (36%), Gaps = 49/332 (14%)

Query: 68 FFGVIGDRFGRKVALTSALFCMSVPTVLIGILPTYHTIGITATILMICVRILQGLS-MGG 126
G + DRFGR+ L +L +V ++ P +L I RI+ G++ G
Sbjct: 62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFL-------WVLYIG-RIVAGITGATG 113

Query: 127 ALTGSISFVI--EHTSHKHQGFTGSISMSSICSGLLLGSFVSYIVKNILTASQFDSFGWR 184
A+ G+ I +H GF + + +G +LG + F
Sbjct: 114 AVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMG-------------GFSPH 160

Query: 185 IPFLLGFFILFAAFYIKKNTHETPNFKNLREQKKILQSPLKKVITNYW--FDILISIFIN 242
PF + F L E K + PL++ N F + +
Sbjct: 161 APFFAAAALNGLNFLT--------GCFLLPESHKGERRPLRREALNPLASFRWARGMTVV 212

Query: 243 APGSIIFYLTTIYLVSFLKISRNFTENEVNSLASVSYVIMIAV-----TLLSGYLSDIIG 297
A +F++ + + F E+ + + + I +A +L ++ +
Sbjct: 213 AALMAVFFIMQLVGQVPAALWVIFGEDRFH-WDATTIGISLAAFGILHSLAQAMITGPVA 271

Query: 298 RR--KIFVINLIIIIVTTSFLLNNFENGAFTSVIISQLILAILAASYIGPEPALQAEL-- 353
R + + L +I T ++L F T ++ I+ +LA+ IG PALQA L
Sbjct: 272 ARLGERRALMLGMIADGTGYILLAF----ATRGWMAFPIMVLLASGGIG-MPALQAMLSR 326

Query: 354 YPTNIRNTALSISYNTATSIFGGTTPLVFEYL 385
R L S TS+ PL+F +
Sbjct: 327 QVDEERQGQLQGSLAALTSLTSIVGPLLFTAI 358


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0117SECGEXPORT566e-14 Protein-export SecG membrane protein signature.
		>SECGEXPORT#Protein-export SecG membrane protein signature.

Length = 110

Score = 56.1 bits (135), Expect = 6e-14
Identities = 32/100 (32%), Positives = 55/100 (55%), Gaps = 1/100 (1%)

Query: 1 MIDILLFVHITIAILLIIVILMQR-SGSDGISSISGGNNMGVVSAKTVGNFLTKSTIILT 59
M + LL V + +AI L+ +I++Q+ G+D +S G + + + GNF+T+ T +L
Sbjct: 1 MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA 60

Query: 60 TLFLINAIVLANLSSKKKSDLVSKINEIEENQAENSLPIA 99
TLF I ++VL N++S K + N + E + P A
Sbjct: 61 TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAA 100


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0130PF08280290.037 M protein trans-acting positive regulator
		>PF08280#M protein trans-acting positive regulator

Length = 530

Score = 28.7 bits (64), Expect = 0.037
Identities = 18/52 (34%), Positives = 33/52 (63%), Gaps = 2/52 (3%)

Query: 218 PNTKIYDQIYNIAIDEFLKQNSNKFDIIFSFCGLSFTNNLIEYFNLIYSSLN 269
P T+I+ Q+ + + + LK++S DII ++C L+F+ ++Y LIY + N
Sbjct: 250 PQTRIFQQLKKLFVYDSLKKSSR--DIIETYCQLNFSAGDLDYLYLIYITAN 299


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0131VACJLIPOPROT2357e-80 VacJ lipoprotein signature.
		>VACJLIPOPROT#VacJ lipoprotein signature.

Length = 251

Score = 235 bits (600), Expect = 7e-80
Identities = 70/201 (34%), Positives = 104/201 (51%), Gaps = 7/201 (3%)

Query: 57 YDPYEKFNRKVFVFN-SVLDYIILRPLAIGYKNITNDYVKARVNSFVSNVDTLLTAVNYG 115
DP E FNR ++ FN +VLD I+RP+A+ +++ + +++F N++ VNY
Sbjct: 29 SDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYF 88

Query: 116 LQLNYDKTMKSVWRFLINTTLGIGGLFDVAGKVGLPSNR---QTFGSTLAHYGVAPGPYL 172
LQ + + M RF +NT LG+GG DVAG R FGSTL HYGV GPY+
Sbjct: 89 LQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYV 148

Query: 173 VLPIIGSTNARDMTDLVITNYALNPLMYYTHNDFDLGVLAVSTINDRYVVLPFSDYVMKN 232
LP GS RD D AL P++ + +G + I R +L SD +++
Sbjct: 149 QLPFYGSFTLRD--DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLD-SDGLLRQ 205

Query: 233 STDPYVAIRSALHRAREASVQ 253
S+DPY+ +R A + +
Sbjct: 206 SSDPYIMVREAYFQRHDFIAN 226


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0134ALARACEMASE2551e-84 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 255 bits (652), Expect = 1e-84
Identities = 101/361 (27%), Positives = 166/361 (45%), Gaps = 21/361 (5%)

Query: 27 TVEINLSTIKNNYLLLQDICKTSLVGAAVKANGYGLGAVQISKALIEENCRHFFVASSEE 86
++L +K N +++ + V + VKAN YG G +I A+ + F + + EE
Sbjct: 6 QASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDG--FALLNLEE 63

Query: 87 GVNLRNALGLDVNILVLNGVFEHDALELI-EYNLTPVLNNLKQIEIWQKFSNLKNRLLPC 145
+ LR G IL+L G F LE+ ++ LT +++ Q++ Q + LK L
Sbjct: 64 AITLRER-GWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQN-ARLKAPL-DI 120

Query: 146 YLHFNTGINRLGLSSDEIEQLINDRDLLKGLDLQYIISHLAISEEIDNPYNLEQLNRFKA 205
YL N+G+NRLG D + + + + ++SH A +E D + R +
Sbjct: 121 YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISG--AMARIEQ 178

Query: 206 YLQYFPNVKASLANSGGIFLGQDYHFDLARSGAALYGLNPLTKN---------PV-TLKA 255
+ + SL+NS + HFD R G LYG +P + PV TL +
Sbjct: 179 AAEGLEC-RRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSS 237

Query: 256 PIIHLQNLTLDSHIGYNMTFTTKRDSVIATLPLGYADGFSRNFSNQGEVFINSRGVPIVG 315
II +Q L +GY +T + + I + GYADG+ R+ V ++ VG
Sbjct: 238 EIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVG 297

Query: 316 RVSMDLINIDVTDLPPSEIFLGQEAEIIGNYCTPDKIASIIGTIGYEVLTNLGSRYKRKY 375
VSMD++ +D+T P + +G E+ G D +A+ GT+GYE++ L R
Sbjct: 298 TVSMDMLAVDLTPCP--QAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVT 355

Query: 376 I 376
+
Sbjct: 356 V 356


2RMA_0237RMA_0274Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0237-1113.033522putative 6-pyruvoyl tetrahydropterin synthase
RMA_02380113.492373dihydrolipoamide succinyltransferase
RMA_0239-192.6665232-oxoglutarate dehydrogenase E1 component
RMA_0242091.771418DNA repair protein RecN
RMA_0243-1101.892467DNA uptake lipoprotein
RMA_02450110.725013chaperone protein DnaJ
RMA_024609-0.680984molecular chaperone DnaK
RMA_0247-210-2.636838heat shock protease
RMA_0250014-3.411055hypothetical protein
RMA_0251-114-3.574304hypothetical protein
RMA_0252010-2.445895DNA polymerase III subunit delta
RMA_0253110-0.449527ubiquinone biosynthesis protein coq7
RMA_025419-0.252795cytochrome c oxidase subunit III
RMA_0255-110-0.898691VirB2-like protein
RMA_0256-19-2.019881hypothetical protein
RMA_0257-210-1.3951012,3,4,5-tetrahydropyridine-2,6-carboxylate
RMA_0260-110-1.813260bifunctional penicillin-binding protein 1C
RMA_0266112-3.417386universal stress protein UspA
RMA_0267111-2.769960TRAP-type transport system, periplasmic protein
RMA_0268011-0.347818hypothetical protein
RMA_02690121.276677ferredoxin
RMA_02700141.717032chaperone protein HscA
RMA_02710173.937359co-chaperone HscB
RMA_02720184.282590ribonuclease HII
RMA_02731184.297488excinuclease ABC subunit B
RMA_02741173.731539GrxC family glutaredoxin
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0245PF06438310.006 Heme acquisition protein HasAp
		>PF06438#Heme acquisition protein HasAp

Length = 205

Score = 31.1 bits (70), Expect = 0.006
Identities = 18/74 (24%), Positives = 27/74 (36%), Gaps = 8/74 (10%)

Query: 71 DAFQNQQSRGGGGNHGGFHPDINDIFGDFFSDFMGGSRRSSRPTSAKVRGSDLKYNLTIN 130
D G N GGF+P G ++ +S+ +A + G DL Y L N
Sbjct: 29 DVNHRPGQVVDGSNTGGFNP--GPFDGSQYA------LKSTASDAAFIAGGDLHYTLFSN 80

Query: 131 LEEAFHGIEKNISF 144
G +I+
Sbjct: 81 PSHTLWGKLDSIAL 94


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0246SHAPEPROTEIN1502e-42 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 150 bits (381), Expect = 2e-42
Identities = 86/389 (22%), Positives = 148/389 (38%), Gaps = 72/389 (18%)

Query: 34 KKTRNLMGKVIGIDLGTTNSCVAVMEG----KEPKVIE-NAEGERTTPSIIAFANGEKLV 88
KK R + + IDLGT N+ + V EP V+ + + S+ A V
Sbjct: 3 KKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA-------V 55

Query: 89 GQSAKRQAVTNPRNTIYAVKRLIGRNFIDPMVRKDQGIVPYNIVKADNGDAWVEADNNKY 148
G AK+ P N I A++ PM KD I + +
Sbjct: 56 GHDAKQMLGRTPGN-IAAIR---------PM--KDGVIADF------------------F 85

Query: 149 SPSQISAFILQKMKETAENYLGDKVTQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIIN 208
++ ++++ + + ++ VP +R+A +++ + AG + +I
Sbjct: 86 VTEKMLQHFIKQVHSNS---FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIE 142

Query: 209 EPTAAALAYGFEKSASKTIAVYDLGGGTFDVSILEIADGVFEVKSTNGDTFLGGEDFDTR 268
EP AAA+ G S + V D+GGGT +V+++ + V + +GG+ FD
Sbjct: 143 EPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVV-----YSSSVRIGGDRFDEA 197

Query: 269 ILNHLIDVFKKENGIDLSKDPLALQRLKEAAEKAKKELSSAVTTD----INLPYITADSS 324
I+N++ + G + AE+ K E+ SA D I +
Sbjct: 198 IINYVRRNYGSLIG-------------EATAERIKHEIGSAYPGDEVREIEVRGRNLAEG 244

Query: 325 GPKHLNIKFTRAELEKLVDDLIEKTIEPCRKALQD-AGFKASDIQE--VVLVGGMTRMPK 381
P+ + + LE L + L + AL+ ASDI E +VL GG +
Sbjct: 245 VPRGFTLN-SNEILEALQEPLTG-IVSAVMVALEQCPPELASDISERGMVLTGGGALLRN 302

Query: 382 VQEAVKKIFGREPHKGVNPDEVVALGAAI 410
+ + + G +P VA G
Sbjct: 303 LDRLLMEETGIPVVVAEDPLTCVARGGGK 331


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0247V8PROTEASE531e-09 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 52.7 bits (126), Expect = 1e-09
Identities = 40/196 (20%), Positives = 78/196 (39%), Gaps = 32/196 (16%)

Query: 56 AVSAYDDTSSWTGTGFINDKNNGYIITNTHVVGGASIGTYFVTFY----------NGEQA 105
+ T ++ +G + K+ ++TN HVV + + + NG
Sbjct: 92 YIQVEAPTGTFIASGVVVGKDT--LLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFT 149

Query: 106 EAKLIYYDIWQDYAVLKVE----SKDIPASATQISFA-SELPKLNQKVFVVGNTEAQGFS 160
++ Y D A++K +K I + + + ++NQ + V G +
Sbjct: 150 AEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDK--- 206

Query: 161 FHTGYLSDLYNIAGLMPQC-TYVINL--NTTGGASGSPVLNDKIEAIGVLYGGGKTHSLA 217
++ ++ G + + +TTGG SGSPV N+K E IG+ +GG +
Sbjct: 207 ----PVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVP-NEF- 260

Query: 218 LHGDYVVRTLESLKNN 233
+ V E+++N
Sbjct: 261 ---NGAVFINENVRNF 273


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0270SHAPEPROTEIN863e-20 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 85.6 bits (212), Expect = 3e-20
Identities = 63/359 (17%), Positives = 122/359 (33%), Gaps = 58/359 (16%)

Query: 23 VGIDFGTTNSLIAIAANRKVKVIKSIDDKELIPTTIDFTSNNFTIGKNKGLRSIKRLFGK 82
+ ID GT N+LI + V S+ + +G + K++ G+
Sbjct: 13 LSIDLGTANTLIYVKGQGIVLNEPSVVAIR--QDRAGSPKSVAAVGHDA-----KQMLGR 65

Query: 83 TLKEILNT-PALFSLVKDYLDVNSSELKLNFANKQLRVPEIAAEVFIYLKNQAEEQ-LKT 140
T I P ++ D+ + ++ + Q
Sbjct: 66 TPGNIAAIRPMKDGVIADF--------------------FVTEKMLQHFIKQVHSNSFMR 105

Query: 141 NLTKAVITVPAHFNDAARGEVMLAAKIAGFEVLRLIAEPTAAAYAYGLNKNQKGCYLVYD 200
+ ++ VP R + +A+ AG + LI EP AAA GL ++ +V D
Sbjct: 106 PSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVD 165

Query: 201 LGGGTFDVSILNIQEGIFQVIATNGDNMLGGNDIDVVITQYLCNKFDLPNSIDTLQLAKK 260
+GGGT +V++++ + + +GG+ D I Y+ + S+ A++
Sbjct: 166 IGGGTTEVAVIS-----LNGVVYSSSVRIGGDRFDEAIINYVRRNY---GSLIGEATAER 217

Query: 261 AKETL-TYKDSFNNDNISINKQTLEQLILPLVERTINIAQECLEQAGNPNID-------- 311
K + + I + + L + + N E L++ +
Sbjct: 218 IKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQ 277

Query: 312 ------------GVILVGGATRIPLIKDELYKAFKIDILSDIDPDKAVVWGAALQAENL 358
G++L GG + + L + I ++ DP V G E +
Sbjct: 278 CPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMI 336


3RMA_0343RMA_0366Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0343-112-3.259398cell division protein ftsA
RMA_0344-311-2.546686membrane protease regulatory membrane protein
RMA_0345-112-2.461549cytochrome c
RMA_0346-113-3.216663hypothetical protein
RMA_0347-112-3.059564UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
RMA_0348-111-2.795800hypothetical protein
RMA_0349-112-0.702478ribonuclease E
RMA_0350-1120.319999cytochrome c oxidase assembly protein
RMA_0351-1122.977129ribosomal large subunit pseudouridine synthase
RMA_0352-1133.698990penicillin-binding protein 4*
RMA_03531165.100196exodeoxyribonuclease III
RMA_03540165.248028pyruvate dehydrogenase e1 component subunit
RMA_03551154.311599pyruvate dehydrogenase subunit beta
RMA_03562142.551811GTP-binding protein TypA
RMA_0357215-0.241186Outer membrane protein
RMA_03582161.084162isocitrate dehydrogenase
RMA_03593161.604134putative monovalent cation/H+ antiporter subunit
RMA_03603151.126280putative monovalent cation/H+ antiporter subunit
RMA_03612152.560439Heme exporter protein B
RMA_03620143.806121hypothetical protein
RMA_03630152.613593ubiquinol-cytochrome c reductase, iron-sulfur
RMA_03640140.853766cytochrome b
RMA_0365-1150.672754endo/excinuclease amino terminal
RMA_0366-1153.283876cytochrome c1, heme protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0343SHAPEPROTEIN492e-08 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 48.6 bits (116), Expect = 2e-08
Identities = 44/190 (23%), Positives = 77/190 (40%), Gaps = 35/190 (18%)

Query: 188 ASAISCLTNDEKNLGSLIIDMGDKTTSFGIFFAGKLIYTGHVNIGSFHISSDI----AKV 243
A+AI + GS+++D+G TT + ++Y+ V IG I +
Sbjct: 146 AAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRN 205

Query: 244 FG--IDLVTAEKLKILYGNAMIPLFEKDSIINMEDFQVDTHHTLNTSVTIYKLAEVIRAR 301
+G I TAE++K G+A + D + +E + + T+ E++ A
Sbjct: 206 YGSLIGEATAERIKHEIGSA----YPGDEVREIEVRGRNLAEGVPRGFTL-NSNEILEAL 260

Query: 302 AEEILSMVKA------------EYDKATKGQVEVFRVVITGGGSQLRGLKELSNKIFEKQ 349
E + +V A D + +G +V+TGGG+ LR L L +
Sbjct: 261 QEPLTGIVSAVMVALEQCPPELASDISERG------MVLTGGGALLRNLDRLLMEE---- 310

Query: 350 SRIGKPEIIA 359
G P ++A
Sbjct: 311 --TGIPVVVA 318


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0345DHBDHDRGNASE270.032 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 27.3 bits (60), Expect = 0.032
Identities = 9/14 (64%), Positives = 12/14 (85%)

Query: 155 ISKPQDIADVILFL 168
++KP DIAD +LFL
Sbjct: 225 LAKPSDIADAVLFL 238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0356TCRTETOQM1914e-55 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 191 bits (487), Expect = 4e-55
Identities = 105/457 (22%), Positives = 188/457 (41%), Gaps = 68/457 (14%)

Query: 4 IRNIAIIAHVDHGKTTLVDNMLKQSGTFRANQAVAE--RAMDSNDLERERGITILAKCTA 61
I NI ++AHVD GKTTL +++L SG +V + D+ LER+RGITI T+
Sbjct: 3 IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS 62

Query: 62 LMWNDIRINIVDTPGHADFGGEVERILSMVDGVVLLVDASEGPMPQTKFVLSKALNLGLK 121
W + ++NI+DTPGH DF EV R LS++DG +LL+ A +G QT+ + +G+
Sbjct: 63 FQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP 122

Query: 122 PIVVINKIDRDDQRIKEVINEVFELFVA----------------------------LEAN 153
I INKID++ + V ++ E A +E N
Sbjct: 123 TIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGN 182

Query: 154 NDQL-----DFPIVYASGRAGRASLTFDDKINP------LDNLADDLAPLFDLIVQHVPK 202
+D L + + + + P +N+ + L ++I
Sbjct: 183 DDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIG--IDNLIEVITNKFYS 240

Query: 203 PLADDKAPFSMLVTTREYNSFFGRVLTGRVQSGTVKINQNVKVLNRENKVLETGRITKIL 262
++ V EY+ R+ R+ SG + + +V++ +E +IT++
Sbjct: 241 STHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-----KITEMY 295

Query: 263 AFRGLERIAINDATAGDIIAVAGVE---NANVADTICSPEVTEAVPSLPIDPPTLSMTFS 319
E I+ A +G+I+ + N+ + DT P P P L T
Sbjct: 296 TSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP--QRERIENP--LPLLQTTVE 351

Query: 320 VNDSPLAGSEGTKVTSSLIGARLMREL-ESNVALKVTEAAEKNAFQVAGRGELQLGILIE 378
+ + + +L E+ +S+ L+ + + ++ G++Q+ +
Sbjct: 352 PSKP----QQREMLLDAL------LEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCA 401

Query: 379 TMRRE-GFELSISRPEVLFQTDEKGNKQEPMEEIQVD 414
++ + E+ I P V++ K E I+V
Sbjct: 402 LLQEKYHVEIEIKEPTVIYMERPLK-KAEYTIHIEVP 437



Score = 29.4 bits (66), Expect = 0.049
Identities = 14/80 (17%), Positives = 26/80 (32%), Gaps = 1/80 (1%)

Query: 406 EPMEEIQVDVDDDYVGVVVKSLALRKAEMTDMRPSGGGKSRVTFIGPSRGLIGYHSQFLT 465
EP ++ +Y+ A + D + + ++ P+R + Y S
Sbjct: 537 EPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQ-LKNNEVILSGEIPARCIQEYRSDLTF 595

Query: 466 ETRGTGIMNRIFHGYADYKG 485
T G + GY G
Sbjct: 596 FTNGRSVCLTELKGYHVTTG 615


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0363PF07675290.010 Cleaved Adhesin
		>PF07675#Cleaved Adhesin

Length = 1358

Score = 29.3 bits (65), Expect = 0.010
Identities = 14/40 (35%), Positives = 19/40 (47%), Gaps = 1/40 (2%)

Query: 54 DLSNIAVGQTVTVKWQGKPIFITNRTPDKIAEARAVKMSE 93
+L+ AVGQ VT+KW P N P+ +SE
Sbjct: 597 NLTGSAVGQKVTLKWSA-PNGTPNPNPNPNPNPGTTTLSE 635


4RMA_0473RMA_0506Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0473211-1.885089dTDP-4-dehydrorhamnose reductase
RMA_0474111-2.034042putative nucleoside-diphosphate sugar epimerase
RMA_0475110-2.869571UDP-N-acetylglucosamine 2-epimerase
RMA_0476211-3.923157hypothetical protein
RMA_0477011-2.959988hypothetical protein
RMA_0478-111-2.863676glycosyltransferase
RMA_0479-113-1.479769RND family efflux transporter MFP subunit
RMA_0480-111-1.466019hypothetical protein
RMA_0481-211-1.258214putative hydrolase/acyltransferase
RMA_0482-210-1.539228glycosyltransferase
RMA_0483-29-2.176576ATPase components of ABC transporters
RMA_048408-3.60880330S ribosomal protein S4
RMA_048519-3.973591protoheme IX farnesyltransferase
RMA_048719-3.969334hypothetical protein
RMA_048818-3.892925Outer membrane assembly protein
RMA_0489-111-4.10103116S rRNA-processing protein RimM
RMA_0490-39-1.498587hypothetical protein
RMA_0491-1153.304361exodeoxyribonuclease VII small subunit
RMA_0496-2164.3376403-methyladenine DNA glycosylase
RMA_0498-1174.185286hypothetical protein
RMA_0499-2143.082432NADH dehydrogenase subunit E
RMA_0501-1142.401332NADH dehydrogenase subunit D
RMA_05020130.691332NADH dehydrogenase subunit C
RMA_0503211-1.445676NADH dehydrogenase subunit B
RMA_0504411-3.160399NADH dehydrogenase subunit A
RMA_0505312-2.756187putative lipid A core - O-antigen ligase
RMA_0506112-3.265879hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0474NUCEPIMERASE543e-10 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 53.6 bits (129), Expect = 3e-10
Identities = 41/247 (16%), Positives = 87/247 (35%), Gaps = 38/247 (15%)

Query: 7 TLMITGGTGSFGNAVLSRFLKS-------DIINDIKEIRIFSRDEKKQEDMRIAFNNPKL 59
++TG G G V R L++ D +ND ++ + ++ P
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSL-------KQARLELLAQPGF 54

Query: 60 KCYIGDVRNYKSIDE--AMHGVDYVFHAAALKQVPTCEFYPMEAINTNVLGAENVLSAAI 117
+ + D+ + + + + A + VF + V P ++N+ G N+L
Sbjct: 55 QFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCR 114

Query: 118 NNKVTKVIVLST---------------DKAVYPINAMGLSKALMEKLAIAKARMRSPGET 162
+NK+ ++ S+ D +P++ +K E +A + + T
Sbjct: 115 HNKIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPAT 174

Query: 163 VLCVTRYGNVMASRGS---VIPLFINQIKQGKELTI-TEPSMTRFLMSLVDSVDLVLYAF 218
L R+ V G + F + +GK + + M R + D + ++
Sbjct: 175 GL---RFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQ 231

Query: 219 EHGHQGD 225
+ D
Sbjct: 232 DVIPHAD 238


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0479RTXTOXIND320.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.5 bits (74), Expect = 0.002
Identities = 16/65 (24%), Positives = 34/65 (52%), Gaps = 4/65 (6%)

Query: 54 DNSRDYYANATGVVEQVSAHQGEIVKKGDILLVID--KNIAETTKSRAAALLNTKQEDYN 111
S++ +V+++ +GE V+KGD+LL + A+T K++++ L ++
Sbjct: 94 GRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQ--T 151

Query: 112 RKEAL 116
R + L
Sbjct: 152 RYQIL 156


5RMA_0707RMA_0770Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0707315-1.178650signal peptide peptidase SppA
RMA_0708518-1.417766integration host factor beta-subunit
RMA_0713420-0.951598integrase
RMA_0714321-0.972271Phage-associated protein
RMA_07153181.039014putative toxin of toxin-antitoxin
RMA_07163210.833313hypothetical protein
RMA_07176222.447884conjugative transfer protein TraE
RMA_07184180.946873hypothetical protein
RMA_07195181.692236hypothetical protein
RMA_07205181.282659F pilus assembly protein TraB
RMA_07216170.418856conjugative transfer protein TraV
RMA_07225170.268877conjugative transfer protein TraC
RMA_0723517-0.686645ATPase family protein
RMA_07268160.668073conjugal DNA transfer protein TraU
RMA_07271016-0.802259conjugative transfer protein TrbC
RMA_0728914-0.428628conjugative transfer protein TraN
RMA_0729914-0.485942F pilus assembly protein TraF
RMA_0730912-0.304521F pilus assembly protein TraH
RMA_0731812-0.504703conjugative transfer protein TraG
RMA_0732713-1.167129hypothetical protein
RMA_0733715-0.394316putative conjugative transfer protein TraD
RMA_0734615-0.578689transposase
RMA_0735616-0.334744conjugal transfer protein TraA
RMA_0736621-0.684348transposase
RMA_0739821-0.858387hypothetical protein
RMA_07407180.153532transposase
RMA_07426170.031426transposase
RMA_0745516-0.414094transposase
RMA_0746621-2.214857transposase
RMA_0747621-2.251632transposase
RMA_0748622-2.597082transposase
RMA_0749622-3.081665transposase
RMA_0750622-3.234891transposase
RMA_0751621-3.000251DNA polymerase III subunit alpha
RMA_0752515-2.333174conjugal transfer protein TraD
RMA_0753413-2.163580guanosine polyphosphate
RMA_0754211-0.926892Signal transduction histidine kinase
RMA_0755212-0.310258site-specific DNA adenine methylase
RMA_07560111.224725hypothetical protein
RMA_07572121.786712*hypothetical protein
RMA_07581111.396262cytidylate kinase
RMA_07592140.70670030S ribosomal protein S1
RMA_0760-3130.081772ATP-dependent Clp protease proteolytic subunit
RMA_0766-113-0.819411*isopentenyl pyrophosphate isomerase
RMA_0767-114-2.437150putative acetyltransferase
RMA_0769015-2.671483acetyltransferase GNAT family protein
RMA_0770214-1.975579hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0708DNABINDINGHU562e-14 Prokaryotic integration host factor signature.
		>DNABINDINGHU#Prokaryotic integration host factor signature.

Length = 91

Score = 56.2 bits (136), Expect = 2e-14
Identities = 17/58 (29%), Positives = 33/58 (56%), Gaps = 1/58 (1%)

Query: 2 MITKNYLIDKIHDKLNYLSKEDVKDSVDLILDYLNESLKEQKRIEIRNFGNFSIRKRK 59
M K LI K+ + L+K+D +VD + ++ L + +++++ FGNF +R+R
Sbjct: 1 MANKQDLIAKVAEATE-LTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERA 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0731FLAGELLIN310.025 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 30.8 bits (69), Expect = 0.025
Identities = 35/261 (13%), Positives = 74/261 (28%), Gaps = 18/261 (6%)

Query: 466 VASSIGTNIADNNLSIDNYSIGNRTIAQQNLTPTLQLAGGIIDDGGMRVTSTDNGRQVLT 525
+ T+ A+NN ++D + T G I G T G
Sbjct: 232 ANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAI----AGAIKGGKEGDTFDYKGVTFTI 287

Query: 526 QAVDSLVNNYRSSKLLQDSYQSQFMSSQSYLDSLNDRYSDLTTTGNSIATEIGKRLSYEE 585
N K+ + + + + + T + +
Sbjct: 288 DTKTGNDGN---GKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTF 344

Query: 586 AKSIGITDSQYLALQQMNSDSTSATDHTGSSNRKGTSTSATGSIGVNTMFGSGMTTVGSS 645
++ L+ N+ + + + ++ TMF + S+
Sbjct: 345 DDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVST 404

Query: 646 NTLDQDQGKSVNQQQSY---NEALSKIKSATKD-----GRLSNANSNVQSLSNNLNSNFS 697
+ + + ALSK+ + R +A +N+ + NLNS S
Sbjct: 405 LINEDAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARS 464

Query: 698 EQQSV--GQEIAQ-TKQNMQQ 715
+ E++ +K + Q
Sbjct: 465 RIEDADYATEVSNMSKAQILQ 485


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0732SYCDCHAPRONE486e-09 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 48.4 bits (115), Expect = 6e-09
Identities = 16/97 (16%), Positives = 38/97 (39%)

Query: 127 DFAEAYNNKGVSYKKLGKYQEAIRLYNIAIKYKPSFAEAYNNKGDTLNSLGKYQEAIENF 186
D E + + + GKY++A +++ + + G ++G+Y AI ++
Sbjct: 34 DTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSY 93

Query: 187 DLALKYAPRYPEAYYNKGISLINLGQHQEAIKNYDIS 223
+ P ++ L+ G+ EA ++
Sbjct: 94 SYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLA 130



Score = 43.8 bits (103), Expect = 2e-07
Identities = 25/131 (19%), Positives = 46/131 (35%), Gaps = 3/131 (2%)

Query: 224 LKYKPDYANAYYNKATSLMQLGKYQEAIKNFDSVIKYQCDNEDAYNLKGCIFSILGKQQE 283
+ D Y+ A + Q GKY++A K F ++ + + G +G+
Sbjct: 29 NEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDL 88

Query: 284 AIKNYDLAIKYKPNFAAAYCNKGTSFRKLGKYQDAIKSYDLA---IKYKSDYAESYLEKG 340
AI +Y + + G+ +A LA I K+++ E
Sbjct: 89 AIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRVS 148

Query: 341 IVLVNLGKYKE 351
+L + KE
Sbjct: 149 SMLEAIKLKKE 159



Score = 42.2 bits (99), Expect = 7e-07
Identities = 21/106 (19%), Positives = 39/106 (36%), Gaps = 3/106 (2%)

Query: 62 EEYFNIGRSLYKLGKYKKAIKNYDLAIKYKTDCAKCYNNKGRALNELANHQEAITNYDLA 121
E+ +++ + Y+ GKY+ A K + ++ + G + + AI +Y
Sbjct: 37 EQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYG 96

Query: 122 IKYDIDFAEAYNNKGVSYKKLGKYQEAIRLYNIA---IKYKPSFAE 164
DI + + G+ EA +A I K F E
Sbjct: 97 AIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKE 142



Score = 41.1 bits (96), Expect = 2e-06
Identities = 18/95 (18%), Positives = 30/95 (31%)

Query: 164 EAYNNKGDTLNSLGKYQEAIENFDLALKYAPRYPEAYYNKGISLINLGQHQEAIKNYDIS 223
E + GKY++A + F + G +GQ+ AI +Y
Sbjct: 37 EQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYG 96

Query: 224 LKYKPDYANAYYNKATSLMQLGKYQEAIKNFDSVI 258
++ A L+Q G+ EA
Sbjct: 97 AIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQ 131



Score = 35.3 bits (81), Expect = 2e-04
Identities = 23/131 (17%), Positives = 43/131 (32%), Gaps = 10/131 (7%)

Query: 178 KYQEAIENF-----DLA--LKYAPRYPEAYYNKGISLINLGQHQEAIKNYDISLKYKPDY 230
+YQ A+E+F +A + + E Y+ + G++++A K +
Sbjct: 10 EYQLAMESFLKGGGTIAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYD 69

Query: 231 ANAYYNKATSLMQLGKYQEAIKNFDSVIKYQCDNEDAYNLKGCIFSILGKQQEAIKNYDL 290
+ + +G+Y AI ++ G+ EA L
Sbjct: 70 SRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFL 129

Query: 291 A---IKYKPNF 298
A I K F
Sbjct: 130 AQELIADKTEF 140



Score = 34.5 bits (79), Expect = 3e-04
Identities = 15/87 (17%), Positives = 28/87 (32%)

Query: 112 QEAITNYDLAIKYDIDFAEAYNNKGVSYKKLGKYQEAIRLYNIAIKYKPSFAEAYNNKGD 171
++A + D + + G + +G+Y AI Y+ + +
Sbjct: 53 EDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAE 112

Query: 172 TLNSLGKYQEAIENFDLALKYAPRYPE 198
L G+ EA LA + E
Sbjct: 113 CLLQKGELAEAESGLFLAQELIADKTE 139



Score = 33.7 bits (77), Expect = 6e-04
Identities = 20/97 (20%), Positives = 32/97 (32%), Gaps = 1/97 (1%)

Query: 263 DNEDAYNLKGCIFSILGKQQEAIKNYDLAIKYKPNFAAAYCNKGTSFRKLGKYQDAIKSY 322
E Y+L GK ++A K + + + G + +G+Y AI SY
Sbjct: 35 TLEQLYSL-AFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSY 93

Query: 323 DLAIKYKSDYAESYLEKGIVLVNLGKYKEAKENFNLA 359
L+ G+ EA+ LA
Sbjct: 94 SYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLA 130



Score = 28.7 bits (64), Expect = 0.024
Identities = 15/86 (17%), Positives = 28/86 (32%)

Query: 304 NKGTSFRKLGKYQDAIKSYDLAIKYKSDYAESYLEKGIVLVNLGKYKEAKENFNLAFKYK 363
+ + + GKY+DA K + + +L G +G+Y A +++
Sbjct: 41 SLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMD 100

Query: 364 SNLIEEYEAIIKALRQLGNDLMANEF 389
+ L Q G A
Sbjct: 101 IKEPRFPFHAAECLLQKGELAEAESG 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0734FLGFLIH353e-04 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 34.8 bits (79), Expect = 3e-04
Identities = 16/38 (42%), Positives = 25/38 (65%)

Query: 277 EGLQVGKAQGIQIGEAKGIQIGEAKGIQIGKAEGKAEE 314
+G Q G A+G Q G +G Q G A+G++ G AE K+++
Sbjct: 54 QGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQ 91



Score = 32.8 bits (74), Expect = 0.001
Identities = 15/36 (41%), Positives = 22/36 (61%)

Query: 267 QQEGKELGILEGLQVGKAQGIQIGEAKGIQIGEAKG 302
++G + GI EG Q G QG Q G A+G++ G A+
Sbjct: 52 HEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEA 87


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0740HTHFIS280.035 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 28.3 bits (63), Expect = 0.035
Identities = 9/22 (40%), Positives = 12/22 (54%)

Query: 103 NLLIFGPSGVGKSHLAAAIGYA 124
L+I G SG GK +A A+
Sbjct: 162 TLMITGESGTGKELVARALHDY 183


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0745HTHFIS280.007 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 27.9 bits (62), Expect = 0.007
Identities = 8/40 (20%), Positives = 19/40 (47%), Gaps = 1/40 (2%)

Query: 18 AEFKESAIKLAIE-SKQPVAQTARELGITKSSLYNWVNKY 56
AE + I A+ ++ + A LG+ +++L + +
Sbjct: 434 AEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIREL 473


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0769SACTRNSFRASE362e-05 Streptothricin acetyltransferase signature.
		>SACTRNSFRASE#Streptothricin acetyltransferase signature.

Length = 173

Score = 35.7 bits (82), Expect = 2e-05
Identities = 17/66 (25%), Positives = 28/66 (42%), Gaps = 5/66 (7%)

Query: 42 FINREGYGVALASLDNTIVGFIAWSKSLLFVSDKTRIHIEVLVIDENYRGKQIGKKLMEY 101
++ EG L L+N +G I + IE + + ++YR K +G L+
Sbjct: 59 YVEEEGKAAFLYYLENNCIGRIKIRSNW-----NGYALIEDIAVAKDYRKKGVGTALLHK 113

Query: 102 LEEIAK 107
E AK
Sbjct: 114 AIEWAK 119


6RMA_0849RMA_0862Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_084939-0.164072hypothetical protein
RMA_085038-0.492343DNA repair protein RecO
RMA_085128-0.290658putative glycoprotein endopeptidase
RMA_085229-0.310691hypothetical protein
RMA_0853281.640055translation initiation factor IF-2
RMA_08541120.510313transcription elongation factor NusA
RMA_08551120.043428hypothetical protein
RMA_0856014-0.189181hypothetical protein
RMA_08593120.790711hemolysin A
RMA_08605161.532360tyrosyl-tRNA synthetase
RMA_0861617-1.020361hypothetical protein
RMA_0862620-0.591752hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0853TCRTETOQM649e-13 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 64.5 bits (157), Expect = 9e-13
Identities = 44/134 (32%), Positives = 62/134 (46%), Gaps = 19/134 (14%)

Query: 336 VMGHVDHGKTSLLDALKSTDIAAGELG------------------GITQHIGAYRVTLAD 377
V+ HVD GKT+L ++L A ELG GIT G +
Sbjct: 8 VLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWEN 67

Query: 378 GRAITFIDTPGHEAFSEMRSRGAKVTDIVIIVVAADDGIKTQTVEAINHAKAAGVPIIVA 437
+ + IDTPGH F R V D I++++A DG++ QT + + G+P I
Sbjct: 68 TK-VNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFF 126

Query: 438 INKIDKPDIDIERV 451
INKID+ ID+ V
Sbjct: 127 INKIDQNGIDLSTV 140


7RMA_1111RMA_1118Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_11113141.669488lipoprotein releasing system, transmembrane
RMA_11124151.738501lipoprotein releasing system ATP-binding protein
RMA_11134151.748115hypothetical protein
RMA_11144162.641706hemolysin-like protein
RMA_11155172.815689cytochrome c-type biogenesis protein ccmF
RMA_11186193.411724outer membrane protein OmpB
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_1118VACCYTOTOXIN320.034 Helicobacter pylori vacuolating cytotoxin signature.
		>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature.

Length = 1291

Score = 31.5 bits (71), Expect = 0.034
Identities = 64/289 (22%), Positives = 95/289 (32%), Gaps = 59/289 (20%)

Query: 419 TGNFTGDASNPGNTAGVITFAANGTLASA-----SADANVAVTNNITAIEASGV---GVV 470
T TG+ + T G A G +AS + D + NI A G
Sbjct: 278 TSKVTGEVNFNHLTVGDHNAAQAGIIASNKTHIGTLDLWQSAGLNIIAPPEGGYKDKPND 337

Query: 471 QLSGTHTAELRLGNAGSVFQLADGTVINGKVNQ-------TAVVGGALAAGAITLDGSAT 523
+ S T + S ++ VIN + T V+ G A G T+
Sbjct: 338 KPSNTTQNNAKNDKQESSQNNSNTQVINPPNSAQKTEIQPTQVIDGPFAGGKNTVVNINR 397

Query: 524 IT----GDIGNGGGAAAL-----------QGITLANDA----------TKTLTLGGANII 558
I G I GG A+L GI L+N A T +T+ G +
Sbjct: 398 INTNADGTIRVGGFKASLTTNAAHLHIGKGGINLSNQASGRSLLVENLTGNITVDGPLRV 457

Query: 559 GSNVGG-TIDFQANGGTIKLTSTQNNILVDFDLDITTDQTGVVDASSLTNA--------- 608
+ VGG + + K + N F+ DI+ + + + T
Sbjct: 458 NNQVGGYALAGSSANFEFKAGTDTKNGTATFNNDISLGRFVNLKVDAHTANFKGIDTGNG 517

Query: 609 --QTLTISGTIGTIGANNKTLGQFNIGSSKTALNGGNVAINELVIGNNG 655
TL SG + N ++ T + N INELV+ NG
Sbjct: 518 GFNTLDFSGVTNKVNINKLI-------TASTNVAVKNFNINELVVKTNG 559



Score = 31.5 bits (71), Expect = 0.041
Identities = 40/165 (24%), Positives = 61/165 (36%), Gaps = 18/165 (10%)

Query: 143 NAQAAATKNAQNVVAPFNGGAA----IANNDLSGVGTIDWGAADSTLVFNLANPTTQKAP 198
+AQ + Q + PF GG I + + GTI G ++L N A+ K
Sbjct: 369 SAQKTEIQPTQVIDGPFAGGKNTVVNINRINTNADGTIRVGGFKASLTTNAAHLHIGKGG 428

Query: 199 LVLGNNA----VIANGVNGTL---------NVTNGFIQVSDKSFATVKAINIGDGQGFIF 245
+ L N A ++ + G + N G+ + KA F
Sbjct: 429 INLSNQASGRSLLVENLTGNITVDGPLRVNNQVGGYALAGSSANFEFKAGTDTKNGTATF 488

Query: 246 NTDATVGNALNLQAGGATINFNGTD-GTGRLVLLSKNGAATDFNV 289
N D ++G +NL+ T NF G D G G L +G N+
Sbjct: 489 NNDISLGRFVNLKVDAHTANFKGIDTGNGGFNTLDFSGVTNKVNI 533


8RMA_1229RMA_1252Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_1229-110-3.447469UDP-glucose 6-dehydrogenase
RMA_1230-17-1.699019hypothetical protein
RMA_123107-0.966493AmpG protein
RMA_123208-1.270213Sec-independent protein translocase protein
RMA_123309-0.750235seryl-tRNA synthetase
RMA_123409-1.015764VirB4
RMA_12351100.250265hypothetical protein
RMA_1236211-0.705445tellurium resistance protein TerC
RMA_12372110.103632hypothetical protein
RMA_12382140.502274putative methyltransferase
RMA_12415161.039806NADH dehydrogenase subunit J
RMA_12423171.887577NADH dehydrogenase subunit K
RMA_12431132.687642NADH dehydrogenase subunit L
RMA_1244-1122.750752NADH dehydrogenase subunit M
RMA_1245-2123.671327cytochrome c biogenesis protein CcmA
RMA_1246-1123.838442NADH dehydrogenase subunit I
RMA_12470134.516778NADH dehydrogenase subunit H
RMA_12480133.741160NADH dehydrogenase subunit G
RMA_12490144.119973hypothetical protein
RMA_1250-1133.426551aconitate hydratase
RMA_1251-1123.090405F0F1 ATP synthase subunit epsilon
RMA_12520123.163793F0F1 ATP synthase subunit beta
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_1231TCRTETB340.002 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 34.1 bits (78), Expect = 0.002
Identities = 32/183 (17%), Positives = 78/183 (42%), Gaps = 8/183 (4%)

Query: 381 LLLIILFIFLYKAADSIPMAMSSP-LFLDLSFTTHEIAVIYKAYGLLIMIVGGALGGVLA 439
+L+ + + + + + + +S P + D + + A+ +L +G A+ G L+
Sbjct: 15 ILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAF-MLTFSIGTAVYGKLS 73

Query: 440 AKMGIFHSVLIGGVIQLLSPLMFMILATIGYDIKPF-ILTVTVQNFCAGFAGTIISIYFA 498
++GI +L G +I ++ +G+ I+ +Q A ++ + A
Sbjct: 74 DQLGIKRLLLFGIIINCFGS----VIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVA 129

Query: 499 SLCNSEFVATQYSIIASFSSLSRIILASLGGICAKYLTWPVFFLGNTLFSMLFIPIFYKI 558
E + +I S ++ + ++GG+ A Y+ W L + +++ +P K+
Sbjct: 130 RYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMI-TIITVPFLMKL 188

Query: 559 YRK 561
+K
Sbjct: 189 LKK 191


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_1234BONTOXILYSIN300.046 Bontoxilysin signature.
		>BONTOXILYSIN#Bontoxilysin signature.

Length = 1196

Score = 30.3 bits (68), Expect = 0.046
Identities = 8/51 (15%), Positives = 22/51 (43%), Gaps = 1/51 (1%)

Query: 132 KVNIKNFNSLINSLSNKLITDFENNYLDSAFQKLDNITNNILNDLNEFGAE 182
++I++ + NS +++ + + Y F +NI+ D++
Sbjct: 810 FLDIQSIKNFFNSQVEQVMKEILSPYQLLLFASKGP-NSNIIEDISGKNTL 859


9RMA_1268RMA_1308Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_1268515-2.402290Iojap-related protein
RMA_1269314-2.205370BolA-like protein
RMA_1270115-1.672692transposase
RMA_1283-2110.538708*translation initiation factor IF-1
RMA_1284-29-0.863666Maf-like protein
RMA_12852122.786489DnaK suppressor-like protein
RMA_12862112.732954site-specific tyrosine recombinase XerC
RMA_12891103.519576phospholipase D superfamily protein PLD
RMA_12942103.788082UbiD family decarboxylase
RMA_12952103.309010parvulin-like peptidyl-prolyl isomerase
RMA_12964124.098785outer membrane protein OmpA
RMA_1297-1121.541948ankyrin repeat-containing protein
RMA_12980131.789900cell division protein FtsK
RMA_13013140.074855methionine aminopeptidase
RMA_1305314-0.811331undecaprenyl-phosphate
RMA_1306215-0.110100hypothetical protein
RMA_1307214-0.178246hypothetical protein
RMA_13082121.325713putative outer surface protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_1296FLAGELLIN377e-04 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 37.3 bits (86), Expect = 7e-04
Identities = 31/290 (10%), Positives = 62/290 (21%), Gaps = 1/290 (0%)

Query: 353 LNGALSQVTGNIGNTNSLATISIGAGTATLGGAVIKATTIDLTDAASVLTLTNANA-VLT 411
L+ N TI + G L + N
Sbjct: 137 LSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYD 196

Query: 412 GAIDNTTGVDNVGVLNLNGALSQVTGNIGNTNSLATISVGAGTATLGGAVIKATTTDLTD 471
+ A + T +
Sbjct: 197 TYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTKST 256

Query: 472 AASVLTLTNANAVLTGAIDNTTGVDNVGVLNLNGALSQVTGNIGNTNSLATISIGAGTAT 531
A + A A+ G +T V + G + T + +++ T
Sbjct: 257 AGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTINGEKVTLTVADIT 316

Query: 532 LGGAVIKATTTDLTDAASVLTLTNANAVLTGAIDNTKGVDNVGVLNLNGALSQVTGNIGN 591
G A + A T + + + + + ++ N S++T N
Sbjct: 317 AGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKITVNGAE 376

Query: 592 TNSLATISVGAGTATLGGAVIKATTIDLTDAASVLTLTNANAVLTGAIDN 641
+ A A+ + + A +ID+
Sbjct: 377 YTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDS 426



Score = 35.4 bits (81), Expect = 0.002
Identities = 44/295 (14%), Positives = 67/295 (22%), Gaps = 5/295 (1%)

Query: 503 LNGALSQVTGNIGNTNSLATISIGAGTATLGGAVIKATTTDLTDAASVLTLTNANAVLTG 562
L+ N TI + G L + N
Sbjct: 137 LSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYD 196

Query: 563 AIDNTKGVDNVGVLNLNGALSQVTGNIGNTNSLATISVGAGTATLGGAVIKATTIDLTDA 622
V V + + VT T A T +DL
Sbjct: 197 TYAVGANKYRVDV----NSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKT 252

Query: 623 ASVLTLTNANAVLTGAIDNTTGVDNVGVLNLNGALSQVTGNIGNTNSLATISVGAGTATL 682
T + GAI D + + TGN GN TI+ G
Sbjct: 253 TKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTIN-GEKVTLT 311

Query: 683 GGAVIKATTIDLTDAASVLTLTNANAVLTGAIDNTKGVDNVGVLNLNGALSQVTGNIGNT 742
+ + V + K + L+ A + V G T
Sbjct: 312 VADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKIT 371

Query: 743 NSLATISIGAGTATLGGAVIKATTIDLTDAASVLTLTNANAVLTGAIDNTTGVDN 797
+ A + A + A S L +A A + +D+
Sbjct: 372 VNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASIDS 426


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_130756KDTSANTIGN310.005 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 30.7 bits (69), Expect = 0.005
Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 13/71 (18%)

Query: 131 YVDFVDLSMFKVFTGAGVG-AAFIKEKINSKDIKDGVTKTFNGTTKNKTNFAYQLSLGTS 189
Y D V F ++ G G G ++ KI++KD+ K T +LG +
Sbjct: 445 YADLVTTESFSIYGGVGAGLGSYTYAKIDNKDV------------KGYTGMVASGALGVA 492

Query: 190 FEVAQGVKAEL 200
A+GV +L
Sbjct: 493 INAAEGVCVDL 503


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_1308OUTRMMBRANEA412e-06 Outer membrane protein A signature.
		>OUTRMMBRANEA#Outer membrane protein A signature.

Length = 346

Score = 40.7 bits (95), Expect = 2e-06
Identities = 33/187 (17%), Positives = 60/187 (32%), Gaps = 21/187 (11%)

Query: 6 KLLLIAATSATILSSSLSFAEGMDNEWYLRIDTGAAMFNEEKDKATGVKLKSNTTVPVDL 65
K IA A ++++ A DN WY G + +++ N
Sbjct: 2 KKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFINNNGPTHENQLGAGAF 61

Query: 66 GIGYYISENCRADLTLGTIIGGKLKKSGAATNAPFTGTNVSASHKPTITRLLINGYVDLT 125
G GY ++ + +G G++ G+ N + V + K
Sbjct: 62 G-GYQVNPYVGFE--MGYDWLGRMPYKGSVENGAYKAQGVQLTAKLGYPIT--------- 109

Query: 126 NFDMFDVFAGAGVGPALVKEKITYNGITGLSSNTKNRTNISYKLTLGTSAQIADGVKVEL 185
D D++ G V T + + G + +T +S G I + L
Sbjct: 110 --DDLDIYTRLGGM---VWRADTKSNVYGKNHDTG----VSPVFAGGVEYAITPEIATRL 160

Query: 186 AYSWIDD 192
Y W ++
Sbjct: 161 EYQWTNN 167


10RMA_0005RMA_0020N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0005-180.192447putative bifunctional glutamate synthase subunit
RMA_0006-1120.076476UDP-N-acetylglucosamine acyltransferase
RMA_00071110.311664(3R)-hydroxymyristoyl-ACP dehydratase
RMA_00082110.012603UDP-3-O-[3-hydroxymyristoyl] glucosamine
RMA_0017411-0.611330*putative P-loop hydrolase
RMA_0018411-0.292554Zinc/manganese ABC transporter substrate binding
RMA_0019311-0.263372Poly(A) polymerase
RMA_00204110.171612cell surface antigen Sca1
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0005NUCEPIMERASE330.007 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 32.8 bits (75), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%), Gaps = 1/32 (3%)

Query: 383 NILVTGL-GPAGFSLSYYLLRSGHNVVAIDGL 413
LVTG G GF +S LL +GH VV ID L
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNL 33


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0006TYPE4SSCAGA290.033 Type IV secretion system CagA exotoxin signature.
		>TYPE4SSCAGA#Type IV secretion system CagA exotoxin signature.

Length = 1147

Score = 28.5 bits (63), Expect = 0.033
Identities = 30/99 (30%), Positives = 47/99 (47%), Gaps = 25/99 (25%)

Query: 49 IEGITEIGENTVIYPFSSIGQPPQILKYANERSSTIIGSNNTIR-EYVTVQAGSQGG--- 104
+EG+ +I N Y F+ Q+L + N SS ++GS+N I E V++ G GG
Sbjct: 277 VEGVADIDPN---YKFN------QLLIHNNALSSVLMGSHNGIEPEKVSLLYGGNGGPGA 327

Query: 105 --------GMVTRVGNNNLFMVGVHIGHDCKIGNNVVFA 135
G + GNN ++ VH+ K G+ +V A
Sbjct: 328 RHDWNATVGYKDQQGNNVATIINVHM----KNGSGLVIA 362


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0018ADHESNFAMILY952e-24 Adhesin family signature.
		>ADHESNFAMILY#Adhesin family signature.

Length = 309

Score = 94.5 bits (235), Expect = 2e-24
Identities = 53/250 (21%), Positives = 102/250 (40%), Gaps = 27/250 (10%)

Query: 51 KQAFAGMMTYLALLFLTFLSL-----TSYAKPKIVVSITPIGSIVSMLVKDKADIESLAI 105
K+ ++ +L+ + L + TS K K+V + + I I + DK D+ S+
Sbjct: 2 KKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIADITKNIAGDKIDLHSIVP 61

Query: 106 SSDCPHHYNLKPSDLVKVKNSDIAIYI--------NEQFDGFAEKLIDNYSKNIIKISDI 157
PH Y P D+ K +D+ Y N F E +K+ +SD
Sbjct: 62 IGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTKLVENAKKTENKDYFAVSDG 121

Query: 158 KSLTTI-------KDNWHIWLDLNNAVILLQEFARIFSENFPELQEDINNNLSTALKELN 210
+ + K++ H WL+L N +I + A+ S P +E NL +L+
Sbjct: 122 VDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLD 181

Query: 211 KLQEIKNNELATI----KDVILLSDSSEYFFLNTNIKTAKLY---SESQTSLRYISKLED 263
KL + ++ I K ++ + +YF + +A ++ +E + + I L +
Sbjct: 182 KLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLVE 241

Query: 264 LIKESNNKCL 273
++++ L
Sbjct: 242 KLRQTKVPSL 251


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0020GPOSANCHOR320.018 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 32.3 bits (73), Expect = 0.018
Identities = 19/157 (12%), Positives = 48/157 (30%), Gaps = 4/157 (2%)

Query: 321 TKNTKSLQKLYMQLTSAQKTIETLKSKAEKIETQIKVGGEDKNKIKKLEKKLTSKNNKAD 380
+L L A +K + +E + K ++K + + +
Sbjct: 186 AALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADS 245

Query: 381 RLFQKIEK--IDIPANKISIESQETVPVTTASTQVSAFQAQQARINEAQQGVLVQKNQQH 438
+ +E + A + +E + ++ + + +A + +
Sbjct: 246 AKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEA--EKADLEHQ 303

Query: 439 IAAEKTKKQRQAEDKQRTEREKKKQEAQKQLSEIKKQ 475
+Q D + KK+ EA+ Q E + +
Sbjct: 304 SQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNK 340



Score = 32.0 bits (72), Expect = 0.026
Identities = 21/193 (10%), Positives = 51/193 (26%)

Query: 324 TKSLQKLYMQLTSAQKTIETLKSKAEKIETQIKVGGEDKNKIKKLEKKLTSKNNKADRLF 383
L + +K +E + + +IK +K ++ + +L A
Sbjct: 147 EAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFS 206

Query: 384 QKIEKIDIPANKISIESQETVPVTTASTQVSAFQAQQARINEAQQGVLVQKNQQHIAAEK 443
+ + + + + A +
Sbjct: 207 TADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELE 266

Query: 444 TKKQRQAEDKQRTEREKKKQEAQKQLSEIKKQEQAIKTAADKAKEVAASAKKETSRTALR 503
+ + K EA+K E +K + ++ A + + SR A +
Sbjct: 267 KALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKK 326

Query: 504 TMQDKMNGYSEQL 516
++ + EQ
Sbjct: 327 QLEAEHQKLEEQN 339


11RMA_0395RMA_0400N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_03952110.491674VirB9
RMA_03960131.156217virulence protein
RMA_0397-1132.491830hypothetical protein
RMA_0398-2121.428534virulence protein
RMA_0399-3120.984905VirB9
RMA_0400-3120.244820VirB10
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0395TYPE4SSCAGX408e-06 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 39.8 bits (92), Expect = 8e-06
Identities = 30/102 (29%), Positives = 48/102 (47%), Gaps = 14/102 (13%)

Query: 162 HPEKYNFNYYISG---SEEIAPIKIFDDGEFTYLQFRDKNAEISGIFAVDDSLRESLVNY 218
+P N+NYY + S+ I P +IFDDG FTY F++ + IF V + S+ +
Sbjct: 395 NPVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQ-PAIFVVQPDGKLSMTD- 452

Query: 219 RLAQDNPNMVIL-------EQVFPKLAIRKGKKVTCVFNQSF 253
A +PNM ++ K + K K + V N+ +
Sbjct: 453 --AAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVTVINKGY 492



Score = 33.6 bits (76), Expect = 6e-04
Identities = 15/33 (45%), Positives = 20/33 (60%)

Query: 61 IELARDEEIVSISMGDTTSWQIVPAGHRIFIKP 93
I+L +DE I I+ G W IVP + IFI+P
Sbjct: 67 IQLEKDETISYITTGFNKGWSIVPNSNHIFIQP 99


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0396PF043351933e-64 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 193 bits (493), Expect = 3e-64
Identities = 42/211 (19%), Positives = 68/211 (32%), Gaps = 2/211 (0%)

Query: 22 KSGEYFIDARKWYNFKYILPLSHRSLLLLICTIFTLLLTLICININILLPTNQKVSYLIK 81
+ YF +A W K + L ++ + L T + + L P Y+I
Sbjct: 9 ELKAYFEEAASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVIT 68

Query: 82 DDAEKQAA-ITNTKHSTLANPYI-SVANIMLQNYVNQREEYNYDILKEQFTFIKNASTSI 139
D A I H Y +V L YV RE + +E F + S
Sbjct: 69 VDRNTGEASIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAREEYFDAVMVMSARP 128

Query: 140 VYMQFANFMHIDNPLSPVIRYQKLYRRSINILSINNINDNEAVVTFESLAKNSTGAILEN 199
+++ F DNP SP + I ++ + N A V F + + + +
Sbjct: 129 EQDRWSRFYKTDNPQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNSTKTD 188

Query: 200 MVWEARIGFIMDSISTSTSPDMPFNFTVTSY 230
V + P + V SY
Sbjct: 189 AVATIKYKVDGTPSKEVDRFKNPLGYQVESY 219


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0398PF043351791e-58 VirB8 type IV secretion protein
		>PF04335#VirB8 type IV secretion protein

Length = 227

Score = 179 bits (456), Expect = 1e-58
Identities = 39/209 (18%), Positives = 82/209 (39%), Gaps = 4/209 (1%)

Query: 36 TQNWYEERSDKLLVQRNLLIILIILLTIFMVISTLVIAFVVKSKQFDPFVIQLNSNTGRA 95
+W ++ + L ++ + + +A + K +P+VI ++ NTG A
Sbjct: 17 AASWERDKLAAAERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEA 76

Query: 96 SVVEPISSP-VLTTDESLTRYFIKKYITARETYNPVDFATIARTTIRLFSTSSVYYNYLG 154
S+ + +T DE++ +YF+ Y+ RE + + + S +
Sbjct: 77 SIAAKLHGDATITYDEAVRKYFLATYVRYREGWIAAAR-EEYFDAVMVMSARPEQDRWSR 135

Query: 155 YIR-NKDFDPTLKYKEENTTFLVIKSWSKIAADKYIVRFSVNETSGNQL-VYNKIAVVSY 212
+ + + P F+ IK S + + V F+ +G+ + +A + Y
Sbjct: 136 FYKTDNPQSPQNILANRTDVFVEIKRVSFLGGNVAQVYFTKESVTGSNSTKTDAVATIKY 195

Query: 213 AYVPMQLTDSELDVNPVGFQVNGYRVDDD 241
+ + NP+G+QV YR D +
Sbjct: 196 KVDGTPSKEVDRFKNPLGYQVESYRADVE 224


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0399TYPE4SSCAGX310.001 Type IV secretion system CagX conjugation protein si...
		>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein

signature.
Length = 522

Score = 31.3 bits (70), Expect = 0.001
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%)

Query: 71 IEFAKNEEIQNIILGDAYAWKITPLANRLFIKPLEKDIRTNM 112
I+ K+E I I G W I P +N +FI+P K +++N+
Sbjct: 67 IQLEKDETISYITTGFNKGWSIVPNSNHIFIQP--KSVKSNL 106



Score = 27.4 bits (60), Expect = 0.029
Identities = 15/58 (25%), Positives = 29/58 (50%), Gaps = 2/58 (3%)

Query: 93 TPLANRLFIKPLEKDIRTNMTIITNKRIYEFDIASTELMMGNERDLVYVIKFYYPKKN 150
+P N + + P + RTN+ + TNK +Y+F + + N +K YP+++
Sbjct: 262 SPEDNSIELSPSDSAWRTNLVVRTNKALYQFILRIAQ--KDNFASAYLTVKLEYPQRH 317


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0400PF03544330.002 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 32.6 bits (74), Expect = 0.002
Identities = 18/82 (21%), Positives = 26/82 (31%), Gaps = 1/82 (1%)

Query: 67 QIPSNIVKPVMEVD-DNIPEIPKLPDPPKLETPTAPPPPPVVKVPPVLPPTTPVEENKDK 125
P +V+P E + P K + P P PV KV PVE
Sbjct: 68 PPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPAS 127

Query: 126 TFPLPPVSLPSTSGTLVESDAE 147
F + P++S +
Sbjct: 128 PFENTAPARPTSSTATAATSKP 149


12RMA_0731RMA_0740N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
RMA_0731812-0.504703conjugative transfer protein TraG
RMA_0732713-1.167129hypothetical protein
RMA_0733715-0.394316putative conjugative transfer protein TraD
RMA_0734615-0.578689transposase
RMA_0735616-0.334744conjugal transfer protein TraA
RMA_0736621-0.684348transposase
RMA_0739821-0.858387hypothetical protein
RMA_07407180.153532transposase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0731FLAGELLIN310.025 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 30.8 bits (69), Expect = 0.025
Identities = 35/261 (13%), Positives = 74/261 (28%), Gaps = 18/261 (6%)

Query: 466 VASSIGTNIADNNLSIDNYSIGNRTIAQQNLTPTLQLAGGIIDDGGMRVTSTDNGRQVLT 525
+ T+ A+NN ++D + T G I G T G
Sbjct: 232 ANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAI----AGAIKGGKEGDTFDYKGVTFTI 287

Query: 526 QAVDSLVNNYRSSKLLQDSYQSQFMSSQSYLDSLNDRYSDLTTTGNSIATEIGKRLSYEE 585
N K+ + + + + + T + +
Sbjct: 288 DTKTGNDGN---GKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTF 344

Query: 586 AKSIGITDSQYLALQQMNSDSTSATDHTGSSNRKGTSTSATGSIGVNTMFGSGMTTVGSS 645
++ L+ N+ + + + ++ TMF + S+
Sbjct: 345 DDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVST 404

Query: 646 NTLDQDQGKSVNQQQSY---NEALSKIKSATKD-----GRLSNANSNVQSLSNNLNSNFS 697
+ + + ALSK+ + R +A +N+ + NLNS S
Sbjct: 405 LINEDAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARS 464

Query: 698 EQQSV--GQEIAQ-TKQNMQQ 715
+ E++ +K + Q
Sbjct: 465 RIEDADYATEVSNMSKAQILQ 485


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0732SYCDCHAPRONE486e-09 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 48.4 bits (115), Expect = 6e-09
Identities = 16/97 (16%), Positives = 38/97 (39%)

Query: 127 DFAEAYNNKGVSYKKLGKYQEAIRLYNIAIKYKPSFAEAYNNKGDTLNSLGKYQEAIENF 186
D E + + + GKY++A +++ + + G ++G+Y AI ++
Sbjct: 34 DTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSY 93

Query: 187 DLALKYAPRYPEAYYNKGISLINLGQHQEAIKNYDIS 223
+ P ++ L+ G+ EA ++
Sbjct: 94 SYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLA 130



Score = 43.8 bits (103), Expect = 2e-07
Identities = 25/131 (19%), Positives = 46/131 (35%), Gaps = 3/131 (2%)

Query: 224 LKYKPDYANAYYNKATSLMQLGKYQEAIKNFDSVIKYQCDNEDAYNLKGCIFSILGKQQE 283
+ D Y+ A + Q GKY++A K F ++ + + G +G+
Sbjct: 29 NEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDL 88

Query: 284 AIKNYDLAIKYKPNFAAAYCNKGTSFRKLGKYQDAIKSYDLA---IKYKSDYAESYLEKG 340
AI +Y + + G+ +A LA I K+++ E
Sbjct: 89 AIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRVS 148

Query: 341 IVLVNLGKYKE 351
+L + KE
Sbjct: 149 SMLEAIKLKKE 159



Score = 42.2 bits (99), Expect = 7e-07
Identities = 21/106 (19%), Positives = 39/106 (36%), Gaps = 3/106 (2%)

Query: 62 EEYFNIGRSLYKLGKYKKAIKNYDLAIKYKTDCAKCYNNKGRALNELANHQEAITNYDLA 121
E+ +++ + Y+ GKY+ A K + ++ + G + + AI +Y
Sbjct: 37 EQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYG 96

Query: 122 IKYDIDFAEAYNNKGVSYKKLGKYQEAIRLYNIA---IKYKPSFAE 164
DI + + G+ EA +A I K F E
Sbjct: 97 AIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKE 142



Score = 41.1 bits (96), Expect = 2e-06
Identities = 18/95 (18%), Positives = 30/95 (31%)

Query: 164 EAYNNKGDTLNSLGKYQEAIENFDLALKYAPRYPEAYYNKGISLINLGQHQEAIKNYDIS 223
E + GKY++A + F + G +GQ+ AI +Y
Sbjct: 37 EQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYG 96

Query: 224 LKYKPDYANAYYNKATSLMQLGKYQEAIKNFDSVI 258
++ A L+Q G+ EA
Sbjct: 97 AIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQ 131



Score = 35.3 bits (81), Expect = 2e-04
Identities = 23/131 (17%), Positives = 43/131 (32%), Gaps = 10/131 (7%)

Query: 178 KYQEAIENF-----DLA--LKYAPRYPEAYYNKGISLINLGQHQEAIKNYDISLKYKPDY 230
+YQ A+E+F +A + + E Y+ + G++++A K +
Sbjct: 10 EYQLAMESFLKGGGTIAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYD 69

Query: 231 ANAYYNKATSLMQLGKYQEAIKNFDSVIKYQCDNEDAYNLKGCIFSILGKQQEAIKNYDL 290
+ + +G+Y AI ++ G+ EA L
Sbjct: 70 SRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFL 129

Query: 291 A---IKYKPNF 298
A I K F
Sbjct: 130 AQELIADKTEF 140



Score = 34.5 bits (79), Expect = 3e-04
Identities = 15/87 (17%), Positives = 28/87 (32%)

Query: 112 QEAITNYDLAIKYDIDFAEAYNNKGVSYKKLGKYQEAIRLYNIAIKYKPSFAEAYNNKGD 171
++A + D + + G + +G+Y AI Y+ + +
Sbjct: 53 EDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAE 112

Query: 172 TLNSLGKYQEAIENFDLALKYAPRYPE 198
L G+ EA LA + E
Sbjct: 113 CLLQKGELAEAESGLFLAQELIADKTE 139



Score = 33.7 bits (77), Expect = 6e-04
Identities = 20/97 (20%), Positives = 32/97 (32%), Gaps = 1/97 (1%)

Query: 263 DNEDAYNLKGCIFSILGKQQEAIKNYDLAIKYKPNFAAAYCNKGTSFRKLGKYQDAIKSY 322
E Y+L GK ++A K + + + G + +G+Y AI SY
Sbjct: 35 TLEQLYSL-AFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSY 93

Query: 323 DLAIKYKSDYAESYLEKGIVLVNLGKYKEAKENFNLA 359
L+ G+ EA+ LA
Sbjct: 94 SYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLA 130



Score = 28.7 bits (64), Expect = 0.024
Identities = 15/86 (17%), Positives = 28/86 (32%)

Query: 304 NKGTSFRKLGKYQDAIKSYDLAIKYKSDYAESYLEKGIVLVNLGKYKEAKENFNLAFKYK 363
+ + + GKY+DA K + + +L G +G+Y A +++
Sbjct: 41 SLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGAIMD 100

Query: 364 SNLIEEYEAIIKALRQLGNDLMANEF 389
+ L Q G A
Sbjct: 101 IKEPRFPFHAAECLLQKGELAEAESG 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0734FLGFLIH353e-04 Flagellar assembly protein FliH signature.
		>FLGFLIH#Flagellar assembly protein FliH signature.

Length = 228

Score = 34.8 bits (79), Expect = 3e-04
Identities = 16/38 (42%), Positives = 25/38 (65%)

Query: 277 EGLQVGKAQGIQIGEAKGIQIGEAKGIQIGKAEGKAEE 314
+G Q G A+G Q G +G Q G A+G++ G AE K+++
Sbjct: 54 QGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQ 91



Score = 32.8 bits (74), Expect = 0.001
Identities = 15/36 (41%), Positives = 22/36 (61%)

Query: 267 QQEGKELGILEGLQVGKAQGIQIGEAKGIQIGEAKG 302
++G + GI EG Q G QG Q G A+G++ G A+
Sbjct: 52 HEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEA 87


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
RMA_0740HTHFIS280.035 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 28.3 bits (63), Expect = 0.035
Identities = 9/22 (40%), Positives = 12/22 (54%)

Query: 103 NLLIFGPSGVGKSHLAAAIGYA 124
L+I G SG GK +A A+
Sbjct: 162 TLMITGESGTGKELVARALHDY 183



 
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