PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome
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A) Input parameters
GenomeDesulfurobacterium_thermolithotrophum_DSM_11699_uid51497_CP002543.gbkThreshold dinucleotide bias2
Threshold codon bias4Threshold %GC bias3
E-value (RPSBlast)0.05Genome (non-pathogenic)
 
B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start    end  
Select Organism     
 
C) Potential GIs and PAIs in CP002543 (download)
S.NoStartEndBiasVirulenceInsertion elementsPrediction
1Dester_0041Dester_0050Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0041311-0.588385methyl-accepting chemotaxis sensory transducer
Dester_0042210-0.329654GTP-binding protein lepA
Dester_0044210-0.250003diguanylate cyclase
Dester_0045211-0.142398response regulator receiver protein
Dester_00462100.128706response regulator receiver modulated CheW
Dester_0047213-0.051223methyl-accepting chemotaxis sensory transducer
Dester_0048215-0.030326CheW protein
Dester_00493120.003972CheA signal transduction histidine kinase
Dester_0050211-0.334035putative myosin-2 heavy chain, non muscle
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0041ANTHRAXTOXNA290.017 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 29.3 bits (65), Expect = 0.017
Identities = 41/184 (22%), Positives = 71/184 (38%), Gaps = 29/184 (15%)

Query: 25 KRMAETIESLVKEIEKVFLKNNSVIAKDVES--LKKISDDLKLFLEDFIPLMRELVKVSV 82
K E + + + K N ++ LKKI D+ LE + L E+ +
Sbjct: 53 KTEKEKFKDSINNLVKTEFTNETLDKIQQTQDLLKKIPKDV---LEIYSELGGEIYFTDI 109

Query: 83 DF-KHL-YESLDAMRKSLEDI--EKIASHTELIAINASIEAARAGEAGRNFAVVANEIRT 138
D +H + L K+ + EK+ + + E R + I+
Sbjct: 110 DLVEHKELQDLSEEEKNSMNSRGEKVPFASRFVF-----------EKKRETPKLIINIKD 158

Query: 139 MARDTFKSVGEVKEIEKEIDEKISRLRNSIDTIDKIKEDVDKLVSGINSIVSISDELDLI 198
A ++ +S KE+ EI + IS D I K K + ++ I S+ SD DL+
Sbjct: 159 YAINSEQS----KEVYYEIGKGISL-----DIISKDKSLDPEFLNLIKSLSDDSDSSDLL 209

Query: 199 YRQQ 202
+ Q+
Sbjct: 210 FSQK 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0042TCRTETOQM1856e-53 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 185 bits (471), Expect = 6e-53
Identities = 124/509 (24%), Positives = 202/509 (39%), Gaps = 111/509 (21%)

Query: 6 IRNFCIIAHIDHGKSTLADRLLEFTGTVSK---RELKEQMLDTLELERERGITIKLNAVR 62
I N ++AH+D GK+TL + LL +G +++ + D LER+RGITI+
Sbjct: 3 IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS 62

Query: 63 MNYEASDGKTYTMHLIDTPGHVDFTYEVSRSLSACEGALLVIDATQGIEAQTIANFFLAL 122
+E + K +++IDTPGH+DF EV RSLS +GA+L+I A G++AQT F
Sbjct: 63 FQWE--NTK---VNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 123 DAGLEIIPVINKIDLPSANVEWVKEQIADVLG---------------------------- 154
G+ I INKID ++ V + I + L
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDT 177

Query: 155 -LDPDDAILA--------------------------------SAKEGIGIKEILEAIVKK 181
++ +D +L SAK IGI ++E I K
Sbjct: 178 VIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNK 237

Query: 182 VPPPSGDVNKPLKALIFDSFYDNYKGVIPFIRVYDGEIKPGMRIKLMSNNKEFEVVEVGT 241
+ L +F Y + + +IR+Y G + + +S ++ ++ E+ T
Sbjct: 238 FYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSV-RISEKEKIKITEMYT 296

Query: 242 QSPN-MIKLDSLKAGEVGWLAANIKNIEDTQVGDTITNAENPTKEPCPGFRPAKPMVFAG 300
+ K+D +GE+ L + +GDT P +E P++
Sbjct: 297 SINGELCKIDKAYSGEIVILQNEFLKLNSV-LGDTKL---LPQRERIEN---PLPLLQTT 349

Query: 301 LYPIDSDRYEDLKEALEKLKLNDAALFFE-PETSAALGFGFRCGFLGLLHMEVIKERLER 359
+ P + E L +AL ++ +D L + + + FLG + MEV L+
Sbjct: 350 VEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIIL----SFLGKVQMEVTCALLQE 405

Query: 360 EFGLELIATAPSVVYKVYLSDGTVIDVQNPAEMPPK--EKIERIEEPYISASIITPAEYV 417
++ +E+ P+V+Y E P K E IE P P +
Sbjct: 406 KYHVEIEIKEPTVIYM---------------ERPLKKAEYTIHIEVP--------PNPFW 442

Query: 418 GSIMQLCQDRRGIQTGFTYLDENRVELRY 446
SI L + +G Y E+ V L Y
Sbjct: 443 ASIG-LSVSPLPLGSGMQY--ESSVSLGY 468



Score = 44.1 bits (104), Expect = 1e-06
Identities = 32/138 (23%), Positives = 54/138 (39%), Gaps = 8/138 (5%)

Query: 350 MEVIKERLERE-FGLELIATAPSVVYKVYLS-DGTVIDVQNPAEMPPKEKIER----IEE 403
ME I+ E+ +G + Y +Y S T D + A + ++ +++ + E
Sbjct: 478 MEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE 537

Query: 404 PYISASIITPAEYVGSIMQLCQDRRGIQTGFTYLDENRVELRYDMPLSEILFDFFDKLKS 463
PY+S I P EY+ T L N V L ++P I ++ L
Sbjct: 538 PYLSFKIYAPQEYLSRAYTDAPKYCANIVD-TQLKNNEVILSGEIPARCI-QEYRSDLTF 595

Query: 464 VSRGYASFDYELAGYKPS 481
+ G + EL GY +
Sbjct: 596 FTNGRSVCLTELKGYHVT 613


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0045HTHFIS882e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.0 bits (218), Expect = 2e-23
Identities = 32/128 (25%), Positives = 57/128 (44%), Gaps = 5/128 (3%)

Query: 6 QNINILTVDDMAAMRKILKTLLAQLGYKNVDEAEDGKQALEILKKNPNKYGLVITDWNMP 65
IL DD AA+R +L L++ GY V + + LV+TD MP
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYD-VRITSNAATLWRWIAAGD--GDLVVTDVVMP 58

Query: 66 NMTGIELVQEIRKDPELKNIPILMVTAEAKKENVLMAIKAGVNNYIVKPFTAETLKEKIE 125
+ +L+ I+K ++P+L+++A+ + A + G +Y+ KPF L I
Sbjct: 59 DENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116

Query: 126 KIFSSLNK 133
+ + +
Sbjct: 117 RALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0046HTHFIS663e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 3e-14
Identities = 25/128 (19%), Positives = 53/128 (41%), Gaps = 16/128 (12%)

Query: 190 KILILDDSPVARKIIRKILENDGHTVFEAQNGIEALQMLHKWLEEAKTTGRDITDYVQLI 249
IL+ DD R ++ + L G+ V N +W+ L+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLW----RWIAAGD---------GDLV 51

Query: 250 ISDIEMPGMDGLTFTRKVKEDTEFSKIPVIINTSLSDRANVDKSRFVGADAHLVK-FDAP 308
++D+ MP + ++K+ +PV++ ++ + K+ GA +L K FD
Sbjct: 52 VTDVVMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109

Query: 309 DLVKLVHQ 316
+L+ ++ +
Sbjct: 110 ELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0047BINARYTOXINA300.024 Clostridial binary toxin A signature.
		>BINARYTOXINA#Clostridial binary toxin A signature.

Length = 454

Score = 30.4 bits (68), Expect = 0.024
Identities = 21/64 (32%), Positives = 36/64 (56%), Gaps = 11/64 (17%)

Query: 5 WEKKEIKRLEEELNSLKQKYQNLQKAYDACEKEKESLKEKLSEFSQ-KNLELSKEIEKLK 63
WEKKE +R+E+ L++L++ +A E K+ E++S +SQ + +IE
Sbjct: 60 WEKKEAERVEKNLDTLEK---------EALELYKKD-SEQISNYSQTRQYFYDYQIESNP 109

Query: 64 KEKE 67
+EKE
Sbjct: 110 REKE 113


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0049PF06580395e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 39.1 bits (91), Expect = 5e-05
Identities = 10/59 (16%), Positives = 20/59 (33%), Gaps = 8/59 (13%)

Query: 383 LVRNALDHGIEPPEERVAKGKPEVGTVKLFAYHEGDHIIVGIQDDGKGIDPEKVKQKAI 441
LV N + HGI P+ G + L + + + +++ G +
Sbjct: 263 LVENGIKHGIAQ--------LPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGT 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0050SALVRPPROT280.024 Salmonella virulence-associated 28kDa protein signature.
		>SALVRPPROT#Salmonella virulence-associated 28kDa protein signature.

Length = 241

Score = 27.8 bits (61), Expect = 0.024
Identities = 34/152 (22%), Positives = 59/152 (38%), Gaps = 14/152 (9%)

Query: 1 MEKSLLQELKELLDLIESFKSEISQISAQKAGFKAINHHIDIAILESEEATKKIIDFIGS 60
M K + KE LD+ + S A GF+ NH D+ I E+ + F G
Sbjct: 44 MRKMPVSHFKEALDVPDYSGMRQSGFFAMSQGFQLNNHGYDVFIHARRESPQSQGKFAGD 103

Query: 61 SL------EAVQESLELISQIKVKEDS---TEKAKRLRELLSATTSSLINALTLL----- 106
+ V ++ + +S + EDS K + +++ SL TL
Sbjct: 104 KFHISVLRDMVPQAFQALSGLLFSEDSPVDKWKVTDMEKVVQQARVSLGAQFTLYIKPDQ 163

Query: 107 EFQDILAQRLLKVKNFLSDIEKSILKIAILAG 138
E A L K + F+ +E + + +++G
Sbjct: 164 ENSQYSASFLHKTRQFIECLESRLSENGVISG 195


2Dester_0295Dester_0311Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_029529-0.699519Type II secretion system F domain
Dester_02962100.241452peptidase U32
Dester_0297214-0.033155hypothetical protein
Dester_0298013-0.542956Histidyl-tRNA synthetase
Dester_0299013-0.8125641-deoxy-D-xylulose 5-phosphate reductoisomerase
Dester_0300012-1.087923ATP:corrinoid adenosyltransferase
Dester_0301113-1.094349hypothetical protein
Dester_0302213-1.158158Radical SAM domain protein
Dester_0303110-0.439757Three-deoxy-D-manno-octulosonic-acid transferase
Dester_030439-0.834497Tetraacyldisaccharide 4'-kinase
Dester_030539-1.087690lipid A biosynthesis acyltransferase
Dester_0306210-1.117485tRNA dimethylallyltransferase
Dester_0307111-1.442565RNA chaperone Hfq
Dester_0308110-1.489593hypothetical protein
Dester_0309114-1.640251Fibronectin-binding A domain protein
Dester_03103161.495228protein of unknown function UPF0005
Dester_03112131.518867hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0295BCTERIALGSPF341e-117 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 341 bits (875), Expect = e-117
Identities = 117/406 (28%), Positives = 221/406 (54%), Gaps = 3/406 (0%)

Query: 1 MAIYTYVGRDILDRKRKGKIEADNEKLAKQLLFSKGIVHI---EKLKEDKSIFKSELDFS 57
MA Y Y D +K +G EAD+ + A+QLL +G+V + E + + + L
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 58 FLNRISTKDKLIFTRQLYAMIHAGISIVTALRIIKEQIQNKSLKKIIEDIASHIEEGGKF 117
R+ST D + TRQL ++ A + + AL + +Q + L +++ + S + EG
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 118 STALSKYKNIFGELYISMIRAAEESGTLEETLKRLAEYLEKIEKLRGKIKSALFYPAFVL 177
+ A+ + F LY +M+ A E SG L+ L RLA+Y E+ +++R +I+ A+ YP +
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 178 LIATIIIGGILIFIIPTFKALYKDLGGELPSLTQFVIELSNFLRDYVGWIVLGLVLTVVL 237
++A ++ +L ++P + + LP T+ ++ +S+ +R + W++L L+ +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 238 LVSLRKFKKARYLMDLTLLRLPIIGQLILKASIASFSRTLSSMVSSGLNILNALSISGET 297
+ + +K R LL LP+IG++ + A ++RTLS + +S + +L A+ ISG+
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 298 TNNEVLRRAINGVRNQVEKGISISVALSRYKVFSPMLINMVAIGEEAGNLDEMLSKVADF 357
+N+ R ++ + V +G+S+ AL + +F PM+ +M+A GE +G LD ML + AD
Sbjct: 301 MSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERAADN 360

Query: 358 YEEEVDRTVDALTSLIEPIMMVFIGGIIGFIIIAMYLPIFKIGELI 403
+ E + L EP+++V + ++ FI++A+ PI ++ L+
Sbjct: 361 QDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTLM 406


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0301ACRIFLAVINRP250.025 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 24.8 bits (54), Expect = 0.025
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)

Query: 4 KALIIRLIAIC-TVLLFSSGCVPLLIAGGAGAAA 36
A+ +RL I T L F G +PL I+ GAG+ A
Sbjct: 965 MAVRMRLRPILMTSLAFILGVLPLAISNGAGSGA 998


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0309FbpA_PF058333033e-98 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 303 bits (777), Expect = 3e-98
Identities = 140/575 (24%), Positives = 257/575 (44%), Gaps = 59/575 (10%)

Query: 1 MDYLYIEKCVKELKERILKERVIKIYSDYRLFSVKIGSSFLNIYTGQPNALFFSNENLVS 60
+D +++ + ELK I+ ++ K+ + + LNI G+ + + +
Sbjct: 3 LDGIFLYSIIDELKNTIINGKIDKVNQPEKDEII------LNIRKGRLSFKLLISSSSNY 56

Query: 61 SELQGFNI------------------IQGTFVRAVKLPVIDRVIEVETVKLLPSGSFELY 102
+ ++ I + + DR++ ++ G +Y
Sbjct: 57 PRIHLTDLTKPNPIKAPMFCMVLRKYISNAKIVDIHQINQDRIVVIDFESTDELGFNSIY 116

Query: 103 FLIFELTGKNANVFLLN-QKRKILSLLREV---KSSIRPISRGEIYTFPPQEKK------ 152
LI E+ G+++N+ L+ + I+ ++ + ++ R I G Y +PP+ K
Sbjct: 117 SLIIEIMGRHSNMTLIRKRDNIIMDSIKHITPDINTYRSIYPGIEYVYPPKSPKLNPFDF 176

Query: 153 --EFQELEFGKVKREGIEKNLHKFVAGISPLNGKEIAYLFEKFG------NLQKAYEEFL 204
+ E + + + K G+S EI + + NL++ E
Sbjct: 177 SYDMIENFTKENSLQLNDNIFSKIFTGVSKTLSSEICFRLKNNSIDLSLSNLKEIVEVCK 236

Query: 205 RKHKESKSAFLYYE-KGKPKYMTTFFYQSLKNLEFKKFSGNFPYTKCWEFYYREKIEKEK 263
KE +S + K F+ +L + E K +K E +Y K + ++
Sbjct: 237 DLFKEIQSNKFEFNCYTKNNSFVGFYCLNLMSKEDYKKIQYDSSSKLLENFYYAKDKSDR 296

Query: 264 VDSLKKSLLKDVEKRIEALKKEIEALKSKEELLKEAEENRKLGELLKYNLNSLKPGKKSV 323
+ S L K V I K+ + L + + ++ + + GELL N+ +LK G +
Sbjct: 297 LKSKSSDLQKIVMNNINRCTKKDKILNNTLKKCEDKDIFKLYGELLTANIYALKKGLSHI 356

Query: 324 KVFDYYTNQ--EIEVPIDPSVSPQKNVEKYFSKYRKLKRKAEYEETLRKKLEEELLEQEF 381
++ +YY+ +++ +D + +P +NV+ Y+ KY KLK+ E + EEEL
Sbjct: 357 ELANYYSENYDTVKITLDENKTPSQNVQSYYKKYNKLKKSEEAANEQLLQNEEELNYLYS 416

Query: 382 LKSSINEKESLEELKSFLPE------------TKEKEKRKKNFKVYILPSGRKIVVGRSS 429
+ ++IN ++ +E++ E K K+ + +I G I VG+++
Sbjct: 417 VLTNINNADNYDEIEEIKKELIETGYIKFKKIYKSKKSKTSKPMHFISKDGIDIYVGKNN 476

Query: 430 RENEFLSLKLANPWDIWFHAKEIPGSHVILRLEKGEKPSEEDIVLSAATAAFFSRGKQSG 489
+N++L+LK AN DIWFH K IPGSHVI++ E ++ +A AA++S+ + S
Sbjct: 477 IQNDYLTLKFANKHDIWFHTKNIPGSHVIVK--NIMDIPESTLLEAANLAAYYSKSQNSS 534

Query: 490 KVAVDYTEVKNLKKPKGAPPGFVVYENEKTIFVNP 524
V VDYTEVKN+KKP GA PG V+Y +TI+V P
Sbjct: 535 NVPVDYTEVKNVKKPNGAKPGMVIYSTNQTIYVTP 569


3Dester_0348Dester_0365Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_03483130.915192hypothetical protein
Dester_03495140.191792hypothetical protein
Dester_03505140.674022hypothetical protein
Dester_03515150.911266hypothetical protein
Dester_03525170.747034cytochrome c family protein
Dester_03535132.328225hypothetical protein
Dester_03545132.721936NHL repeat containing protein
Dester_03554113.434564cytochrome C family protein
Dester_03562113.248735hypothetical protein
Dester_03572122.782805hypothetical protein
Dester_03582112.578902hypothetical protein
Dester_03590140.622335hypothetical protein
Dester_0360-2120.279912Integrase catalytic region
Dester_0361-1101.628968****Glucosamine--fructose-6-phosphate
Dester_0362-1102.362000formyltetrahydrofolate deformylase
Dester_0363-2113.081276ATP phosphoribosyltransferase
Dester_0365-2143.178782UDP-N-acetylglucosamine1-
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0360HTHFIS290.029 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.029
Identities = 7/30 (23%), Positives = 12/30 (40%), Gaps = 3/30 (10%)

Query: 57 GNARKTCRYFGISPTTFYKWKKRYDKYGIE 86
GN K G++ T K+ + G+
Sbjct: 450 GNQIKAADLLGLNRNTLR---KKIRELGVS 476


4Dester_0409Dester_0414Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_04092130.053010DNA-(apurinic or apyrimidinic site) lyase
Dester_04102120.5160702-dehydro-3-deoxyphosphooctonate aldolase
Dester_0411214-0.158129sugar-phosphate isomerase, RpiB/LacA/LacB
Dester_04122130.882995Thioredoxin domain-containing protein
Dester_04134141.160398carbon monoxide dehydrogenase accessory protein,
Dester_04143121.135169ferredoxin
5Dester_0587Dester_0599Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0587017-3.096419Phosphoribosylanthranilate isomerase
Dester_0588126-5.408753heat shock protein Hsp20
Dester_0589122-4.408654ABC transporter related protein
Dester_0590123-4.529078SufBD protein
Dester_0591124-3.489500Desulfoferrodoxin ferrous iron-binding region
Dester_0592023-3.214859DNA methylase N-4/N-6 domain protein
Dester_0593020-1.359762type III restriction protein res subunit
Dester_0594-2182.626386transposase mutator type
Dester_0595-2151.839925riboflavin synthase, alpha subunit
Dester_0596-1121.813938transposase IS116/IS110/IS902 family protein
Dester_0597-190.507046Ppx/GppA phosphatase
Dester_059819-0.204238nucleotide sugar dehydrogenase
Dester_0599211-0.466557CDP-alcohol phosphatidyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0592PF01540320.008 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

Length = 475

Score = 32.4 bits (73), Expect = 0.008
Identities = 56/254 (22%), Positives = 107/254 (42%), Gaps = 24/254 (9%)

Query: 186 ELKEVKEDGTIVLKVLYSERGKKTKE--KEIIKQVKKVYKHSRLTEEYLNKAIKTFEKQA 243
EL E+K + L +E +K KE KE++K +K+ + + K + F+
Sbjct: 226 ELAEIKAEDDKKL----AEENQKIKEGAKELLKLSEKIQSFADTIALTITKLERKFQIDE 281

Query: 244 NVDYFINKNAEKFLKEQFDLWMYQYLFSQEASFDLKRFQELQALKNIAYRIIEFIAQFED 303
+ E K+ ++ + + + + F L EL++ K +E I +
Sbjct: 282 KFKKQLISTIELLNKKSVEVKTFATVNTIKKDFLL---SELESFKEFNTSWLEKIVSEWE 338

Query: 304 ELRKIWEKPRLVFNSNYVITL-DRIANKEEGKEILEEIISQLKEQEKEFKKSINQLKKIK 362
E++K W K + L + + G E L++I ++ E K K+I +L+K
Sbjct: 339 EVKKAWSKELAEIKAEDDKKLAEENQKIKNGVEELKKINNEAFELSKTVNKTIAELEKKF 398

Query: 363 ENYKSYKERFENH-----QIKNQLEEWYLLDIIEEGFGVDGILKNGELNEKWKFLPVDTK 417
+ S+KE+ +N Q++E+ + +EGF L E F + T
Sbjct: 399 KIDVSFKEQLKNFADDLLDKSRQIDEFTTVTSTQEGF---------TLAELESFKEITTT 449

Query: 418 YFNGLKEKIEEIFD 431
+FNG+K + + +
Sbjct: 450 WFNGMKSEWARVQE 463


6Dester_0640Dester_0655Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0640-114-3.235443rfaE bifunctional protein
Dester_0641-212-3.372729PP-loop domain protein
Dester_0642-112-4.463319protein of unknown function DUF178
Dester_0644012-2.163431hypothetical protein
Dester_0645012-1.528844hypothetical protein
Dester_0646112-0.170929class II aldolase/adducin family protein
Dester_0647112-1.124640Prephenate dehydrogenase
Dester_0648012-0.794366TonB family protein
Dester_0649-1100.201269aspartyl-tRNA synthetase
Dester_0650-113-2.534304Ferritin Dps family protein
Dester_0651-114-3.540413hypothetical protein
Dester_0652-115-4.279376Lysozyme subfamily 2
Dester_0653015-3.512758hydro-lyase, Fe-S type, tartrate/fumarate
Dester_0654-115-4.188953L-seryl-tRNA(Sec) selenium transferase
Dester_0655-111-3.022828TonB-dependent receptor
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0648PF03544757e-18 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 74.6 bits (183), Expect = 7e-18
Identities = 14/81 (17%), Positives = 37/81 (45%), Gaps = 5/81 (6%)

Query: 210 IEKKKFYPRLAKRFGIEGKVILKIVIDRKGNLESVSIVKTSGSKVLDKAALKLIKKCKFP 269
+ YP A+ IEG+V +K + G +++V I+ + + ++ +++ ++
Sbjct: 161 SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYE 220

Query: 270 PLPPEYKKDDFEVEIPIRYEL 290
P P + + I +++
Sbjct: 221 PGKPGSG-----IVVNILFKI 236


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0650HELNAPAPROT333e-04 Helicobacter neutrophil-activating protein A family ...
		>HELNAPAPROT#Helicobacter neutrophil-activating protein A family

signature.
Length = 153

Score = 32.5 bits (74), Expect = 3e-04
Identities = 25/148 (16%), Positives = 60/148 (40%), Gaps = 10/148 (6%)

Query: 13 ADKIIELLNKALCDEWLAYYQ----YWIGSKVVKGPMKDAVIAELIQHANDELRHADMLV 68
+ LN L + +L Y + +W VKGP + + + + D +
Sbjct: 10 QTLVENSLNTQLSNWFLLYSKLHRFHW----YVKGPHFFTLHEKFEELYDHAAETVDTIA 65

Query: 69 TRILELGGTPVLSPKQWFELTNCGYDAPVDPFVKIVLEQNIKGEQCAIDTYSEILKIT-K 127
R+L +GG PV + K++ E + D + +++ + + ++ + +
Sbjct: 66 ERLLAIGGQPVATVKEYTEHASIT-DGGNETSASEMVQALVNDYKQISSESKFVIGLAEE 124

Query: 128 DIDPITYNIALQILTDEVEHEEDLQSFL 155
+ D T ++ + ++ + + L S+L
Sbjct: 125 NQDNATADLFVGLIEEVEKQVWMLSSYL 152


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0652FLGFLGJ399e-06 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 39.3 bits (91), Expect = 9e-06
Identities = 28/92 (30%), Positives = 43/92 (46%), Gaps = 25/92 (27%)

Query: 144 ELLRRVNSVPVSLVLAQGAIESGWGTSRFFIE----GNNVFGMYAF-------------- 185
+L + + VP L+LAQ A+ESGWG + E N+FG+ A
Sbjct: 161 QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTE 220

Query: 186 YTTN--KKLKARNGNVYLKVYDDILQSVEDYI 215
Y KK+KA+ +VY L+++ DY+
Sbjct: 221 YENGEAKKVKAK-----FRVYSSYLEALSDYV 247


7Dester_0665Dester_0693Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0665112-3.532652NAD(+) synthase (glutamine-hydrolyzing)
Dester_0666212-3.834636surface antigen variable number
Dester_0668212-2.514945diacylglycerol kinase
Dester_0669112-2.165162metal dependent phosphohydrolase
Dester_0670111-2.515822PhoH family protein
Dester_0671212-3.189637SMC domain protein
Dester_0672110-2.581178hypothetical protein
Dester_0673011-2.550624sodium:neurotransmitter symporter
Dester_067419-3.580886Radical SAM domain protein
Dester_067517-3.165410surface antigen (D15)
Dester_0676210-1.706518hypothetical protein
Dester_0677110-0.970647hypothetical protein
Dester_0678313-0.371781protein of unknown function DUF507
Dester_06793150.121082Tetratricopeptide TPR_1 repeat-containing
Dester_06803211.393191UvrABC system protein B
Dester_06813331.906442Integrase catalytic region
Dester_06832240.988962Resolvase domain
Dester_06841211.740592transposase, IS605 OrfB family
Dester_06862122.353029***Integrase catalytic region
Dester_06871152.950084UPF0434 protein ycaR
Dester_06881112.785650hypothetical protein
Dester_06890112.656117Ribosomal RNA large subunit methyltransferase N
Dester_06900103.100324oxidoreductase FAD/NAD(P)-binding domain
Dester_06912112.900839glutamate synthase (NADPH), homotetrameric
Dester_0692291.285126transposase IS605 OrfB
Dester_0693390.319391translation initiation factor IF-2
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0671GPOSANCHOR512e-08 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 50.8 bits (121), Expect = 2e-08
Identities = 46/277 (16%), Positives = 90/277 (32%), Gaps = 12/277 (4%)

Query: 460 EKEKFLLDSKKEIDNSIKVL-KQLKKKKEKLKELMDSLEKKVEKFLQLEERKNQLIRDRE 518
K L + K + + L ++L KEKL++ SL +K K +LE RK L + E
Sbjct: 71 LKNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALE 130

Query: 519 RFLEEVKELKNKLNKLSFNYDRLRKTEELFTSLKEQVAALEEKKKNLEREAKELETKKAN 578
+ K+ L L++ + + K LE +KA
Sbjct: 131 GAMNFSTADSAKIKTLE---AEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 187

Query: 579 LSKNYD--TKLKEELQNRLKSLKTKEFFFLNKIKKIKEEHNLCVSSAKEVDIKLKELETT 636
L K E N + K + + + +
Sbjct: 188 LEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAK 247

Query: 637 KEKLNKIQGEARNLNFYYGEKKKKEDLIKSLEKEVLSVQEEIEKLNYSEEEYLTLEETYE 696
+ L + + + E ++ + +I+ L + + E
Sbjct: 248 IKTLEAEKAALEA------RQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLE 301

Query: 697 KQKERVESLEKELSRELGKLETLEKQLKVNIKKLKQK 733
Q + + + + L R+L +KQL+ +KL+++
Sbjct: 302 HQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQ 338



Score = 50.1 bits (119), Expect = 3e-08
Identities = 69/387 (17%), Positives = 134/387 (34%), Gaps = 17/387 (4%)

Query: 353 KKELRETEKVFKELKTLEKSFREITIRLSDKQKDLKSLDERIRELPRIEKAKELSKKKKD 412
+K +K E TL+ +++ + L E + EK ++ K +
Sbjct: 53 EKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNA--KEKLRKNDKSLSE 110

Query: 413 TLKELENTDKEIATIEAKLEEVEKRIK-ILESSESLSCPVCGSPMDKVEKEKFLLDSKKE 471
+++ + A +E LE ++L K + EK L +
Sbjct: 111 KASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNF 170

Query: 472 IDNSIKVLKQLKKKKEKLKELMDSLEKKVEKFLQLEERKNQLIRDRERFLEEVKELKNKL 531
+K L+ +K L+ LEK +E + + I+ E + K L
Sbjct: 171 STADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADL 230

Query: 532 NKL-SFNYDRLRKTEELFTSLKEQVAALEEKKKNLEREAKELETKKANLSKNYD--TKLK 588
K + +L+ + AALE ++ LE+ + S K
Sbjct: 231 EKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEK 290

Query: 589 EELQNRLKSLKTKEFFFLNKIKKIKEEHNLCVSSAKEVDIKLKELETTKEKLNK-IQGEA 647
L+ L+ + + ++ + + + K+++ + ++LE + Q
Sbjct: 291 AALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLR 350

Query: 648 RNLNFYYGEKKKKEDLIKSLEKEVLSVQEEIEKLNYSEEEYLTLEETYEKQKERVESLEK 707
R+L+ KK+ E + LE++ SE +L + +E + +EK
Sbjct: 351 RDLDASREAKKQLEAEHQKLEEQNKI----------SEASRQSLRRDLDASREAKKQVEK 400

Query: 708 ELSRELGKLETLEKQLKVNIKKLKQKE 734
L KL LEK K + K E
Sbjct: 401 ALEEANSKLAALEKLNKELEESKKLTE 427



Score = 42.4 bits (99), Expect = 7e-06
Identities = 54/371 (14%), Positives = 118/371 (31%), Gaps = 25/371 (6%)

Query: 216 RAELAQEKALLDNQEKLCKNYREKRNEYNKLRESLRNLKNLLEGTTEEVKRLNQKIVEIE 275
R++ + + + +K + + + L + + LK+ + TEE+ +K+ + +
Sbjct: 46 RSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKND 105

Query: 276 KQQALIPQIEKELKILDPLKMIRAILDEISQLRLEEKEIESKLKELENIEKELLELSKKV 335
K + EK KI + + + +K+K LE + L +
Sbjct: 106 KSLS-----EKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADL 160

Query: 336 PKLKETFRETELLLEHKKKELRETEKVFKELKTLEKSFREITIRLSDKQKDLKSLDERIR 395
K E K K L + + + + E + S E +
Sbjct: 161 EKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEK 220

Query: 396 ELPRIEKAKELSKKKKDTLKELENTDKEIATIEAKLEEVEKRIKILESSESLSCPVCGSP 455
+ +L K + + +I T+EA+ +E R LE +
Sbjct: 221 AALA-ARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALE--------- 270

Query: 456 MDKVEKEKFLLDSKKEIDNSIKVLKQLKKKKEKLKELMDSLEKKVEKFLQLEERKNQLIR 515
F +I L+ +K L+ L + + + + +
Sbjct: 271 ----GAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKK 326

Query: 516 DRERFLEEVKELKNKLNKLSFNYDRLRKTEELFTSLKEQVAALEEKKKNLEREAKELETK 575
E ++++E + + +E LE + + LE + K E
Sbjct: 327 QLEAEHQKLEEQNKISEA------SRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEAS 380

Query: 576 KANLSKNYDTK 586
+ +L ++ D
Sbjct: 381 RQSLRRDLDAS 391



Score = 35.0 bits (80), Expect = 0.001
Identities = 34/241 (14%), Positives = 87/241 (36%), Gaps = 11/241 (4%)

Query: 500 VEKFLQLEERKNQLIRDRERFLEEVKELKNKLNKLSFNYDRLRKTEELFTSLKEQVAALE 559
+ +++ER ++ + + +L L + D L + + KE++ +
Sbjct: 49 TDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELS---NAKEKLRKND 105

Query: 560 EKKKNLEREAKELETKKANLSKNYDTKLKE--ELQNRLKSLKTKEFFFLNKIKKIKEEHN 617
+ + +ELE +KA+L K + + ++K+L+ ++ + +++
Sbjct: 106 KSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALE 165

Query: 618 LCVSSAKEVDIKLKELETTKEKLNKIQGEARNLNFYYGEKKKKEDLIKSLEKEVLSVQEE 677
++ + K+K LE K L Q E + + + ++ +++ E
Sbjct: 166 GAMNFSTADSAKIKTLEAEKAALEARQAELEK------ALEGAMNFSTADSAKIKTLEAE 219

Query: 678 IEKLNYSEEEYLTLEETYEKQKERVESLEKELSRELGKLETLEKQLKVNIKKLKQKESDI 737
L + + E + K L E LE + +L+ ++ +
Sbjct: 220 KAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTAD 279

Query: 738 K 738

Sbjct: 280 S 280


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0680SECA330.005 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 32.9 bits (75), Expect = 0.005
Identities = 16/64 (25%), Positives = 35/64 (54%), Gaps = 1/64 (1%)

Query: 425 RKTEGQIDHLISEIKKRVEKNERILITTLTKKSAEELSKYLLEKGIKAKYMHSEIDSVER 484
+I +I +IK+R K + +L+ T++ + +E +S L + GIK ++++ + E
Sbjct: 429 MTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANE- 487

Query: 485 VEII 488
I+
Sbjct: 488 AAIV 491


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0686HTHFIS290.030 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.030
Identities = 7/30 (23%), Positives = 12/30 (40%), Gaps = 3/30 (10%)

Query: 57 GNARKTCRYFGISPTTFYKWKKRYDKYGIE 86
GN K G++ T K+ + G+
Sbjct: 450 GNQIKAADLLGLNRNTLR---KKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0693TCRTETOQM831e-18 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 83.4 bits (206), Expect = 1e-18
Identities = 68/282 (24%), Positives = 104/282 (36%), Gaps = 80/282 (28%)

Query: 389 ITVMGHVDHGKTTLLDYI-----RNTKVAEREAG-------------GITQHIGASVVEV 430
I V+ HVD GKTTL + + T++ + G GIT I +
Sbjct: 6 IGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGIT--IQTGITSF 63

Query: 431 DTSEGKKKLVFLDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTVEAINHAKAAGV 490
K ++ DTPGH F A R V D A+L+++A DGV QT + + G+
Sbjct: 64 QWENTKVNII--DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121

Query: 491 PIIVAINKIDKPGANPDRVKQE----LTQHGLI-----------------AEDWG----- 524
P I INKID+ G + V Q+ L+ +I +E W
Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG 181

Query: 525 ---------------------------GDTVMVPV---SAKTGEGVDELLEMIALQAELM 554
+ + PV SAK G+D L+E+I +
Sbjct: 182 NDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVIT--NKFY 239

Query: 555 ELKANPDKPARGVVLEAKLDRQRGPVATLLIQSGTLKQGDAI 596
G V + + +R +A + + SG L D++
Sbjct: 240 SSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSV 281


8Dester_0781Dester_0796Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0781214-0.792611UvrABC system protein C
Dester_0782115-0.0282076-pyruvoyl tetrahydropterin synthase and
Dester_0783217-0.042535transposase IS116/IS110/IS902 family protein
Dester_0784210-1.590652methyltransferase
Dester_07850150.235399outer membrane lipoprotein carrier protein LolA
Dester_07860181.423340MotA/TolQ/ExbB proton channel
Dester_07870182.254763Biopolymer transport protein ExbD/TolR
Dester_07881233.530554TonB family protein
Dester_07891264.522474Ribosomal RNA small subunit methyltransferase A
Dester_07902315.951483hypothetical protein
Dester_07913336.479107Nitrilase/cyanide hydratase and apolipoprotein
Dester_07924356.971608Peptide methionine sulfoxide reductase msrA
Dester_07932346.1960172-dehydropantoate 2-reductase
Dester_07943325.250280hypothetical protein
Dester_07951244.265281MutS2 protein
Dester_0796-1223.900896hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0788PF03544514e-10 Gram-negative bacterial tonB protein
		>PF03544#Gram-negative bacterial tonB protein

Length = 243

Score = 51.1 bits (122), Expect = 4e-10
Identities = 46/235 (19%), Positives = 81/235 (34%), Gaps = 43/235 (18%)

Query: 6 RFLLAFILALFLE----AGFIYIVKNLH--KPHKQEVKQVIKISLLKNEKKPKVLVTKQK 59
RF +L++ + AG +Y + P + V ++ E V +
Sbjct: 13 RFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEP 72

Query: 60 KI--------VKEPKQKKKVVIPKEKPKVVTKKPQKKIPQEEKKEV----------LEKK 101
+ + EP ++ VVI K KPK K K ++ K++V E
Sbjct: 73 VVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENT 132

Query: 102 KPGGLKPL----QGNLPAYYVDAIKKAIEE-NIFYPLEAIERGEEGVVSVKFVLDKSG-- 154
P + P V + +A+ YP A EG V VKF + G
Sbjct: 133 APARPTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRV 192

Query: 155 ---KVIKCKPFSGKSEILQKATCIAIERAKFPPIPQTIKNE-----RITFHLEIE 201
+++ KP + + ++ A+ R ++ P +I EI+
Sbjct: 193 DNVQILSAKP----ANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0795IGASERPTASE405e-05 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 39.7 bits (92), Expect = 5e-05
Identities = 29/183 (15%), Positives = 59/183 (32%), Gaps = 11/183 (6%)

Query: 494 EEVIETARSLMTAEDRLAEDIIAALEKEYRKLTEEKETVE---RLKEALKKKEEELQKKE 550
E V E ++ ++ +D + E K V+ + E + E + +
Sbjct: 1038 ETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQT 1097

Query: 551 KELKEKSAREIEKFIEELKKKSEEVFKKAKSEKAKQEIRQIVI-----TAKNRAKALAEA 605
E KE + E E+ + +K++EV K KQE + V +N +
Sbjct: 1098 TETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKE 1157

Query: 606 KEVREVKPGNTVKLLKSGRKGKVLEVDK---ERKVAKVLVGGLKVDVKLSQIEPVEETVK 662
+ + +T + K V + V+ +Q E+
Sbjct: 1158 PQSQTNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSN 1217

Query: 663 QEE 665
+ +
Sbjct: 1218 KPK 1220


9Dester_0824Dester_0839Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0824211-0.237290molybdenum cofactor biosynthesis protein A
Dester_0825-190.172703molybdenum cofactor synthesis domain protein
Dester_08260161.544089formate dehydrogenase family accessory protein
Dester_08270171.416290molybdopterin-guanine dinucleotide biosynthesis
Dester_0828-3121.903361Molybdate-transporting ATPase
Dester_0829-2121.930729binding-protein-dependent transport systems
Dester_0830-2111.312681tungstate ABC transporter binding protein WtpA
Dester_0831-2111.649219transposase IS116/IS110/IS902 family protein
Dester_08324222.121877Dinitrogenase iron-molybdenum cofactor
Dester_08335242.752654carbon-monoxide dehydrogenase, catalytic
Dester_08346252.410332hypothetical protein
Dester_08355212.240329transcriptional regulator, Crp/Fnr family
Dester_08364224.33977410 kDa chaperonin
Dester_08374213.80055160 kDa chaperonin
Dester_08392152.227585Glycine hydroxymethyltransferase
10Dester_0862Dester_0880Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0862212-0.308586Lipoprotein signal peptidase
Dester_08631121.049902DNA ligase
Dester_08640140.956612outer membrane efflux protein
Dester_0865-2140.800322drug resistance transporter, EmrB/QacA
Dester_0866-2110.741721secretion protein HlyD family protein
Dester_0867-2132.070984Tetratricopeptide TPR_1 repeat-containing
Dester_0868-2142.625159transposase, IS605 OrfB family
Dester_0869-1132.020611hypothetical protein
Dester_0870-1183.402298Peptidase M23
Dester_0871-1214.319930Diaminopimelate decarboxylase
Dester_08720224.623337ATP-grasp fold domain protein, DUF201-type
Dester_08730193.609279hypothetical protein
Dester_08740183.270056hypothetical protein
Dester_08750173.034112Protein of unknown function DUF2202
Dester_08762162.731760integral membrane sensor signal transduction
Dester_08772172.960454two component, sigma54 specific, transcriptional
Dester_08782152.496300protein of unknown function DUF1009
Dester_08792163.302283NusB antitermination factor
Dester_08801163.3733626,7-dimethyl-8-ribityllumazine synthase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0864RTXTOXIND300.023 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.8 bits (67), Expect = 0.023
Identities = 8/57 (14%), Positives = 22/57 (38%)

Query: 149 LSEAKSAVEIAESYLQAARRHLKDVKAFFDEGIVPKRDLLEAKVRVRDAEEQLEKAR 205
+ + E+ + + L D + + + K +LE + + +A +L +
Sbjct: 216 RLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYK 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0865TCRTETB1231e-32 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 123 bits (311), Expect = 1e-32
Identities = 90/406 (22%), Positives = 165/406 (40%), Gaps = 28/406 (6%)

Query: 17 FMTLLDTTIVDIVLPHMMSTFEAKPDDIQWVITSYMIASAIAMPVVGWLGGKIGHRNTYL 76
F ++L+ ++++ LP + + F P WV T++M+ +I V G L ++G + L
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 77 LGIGLFTTMSTLCGIAPN-LETMILGRVFQGIGEGLAVPMSMTLLFELFPPEKRGIAMGM 135
GI + S + + + +I+ R QG G + M ++ P E RG A G+
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 136 FALGATFGPSLGPTIGGYLTEHLDWRWVFYVNLLPGILVIYLLMLLIKDDRKKHVADGKL 195
G +GP IGG + ++ W ++ L+P I +I + L+K +K+ G
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHYIHWSYLL---LIPMITIITVP-FLMKLLKKEVRIKGHF 199

Query: 196 DILGFILLAISLSSLITALSKGNDWGWSDEKTVLLLYTFSVSLILFILVELKVENPLVNL 255
DI G IL+++ + + + + L +S ++F+ KV +P V+
Sbjct: 200 DIKGIILMSVGIVFFMLF-TTSYSISF--------LIVSVLSFLIFVKHIRKVTDPFVDP 250

Query: 256 GLFKFTFFRYPVFSLTLFGMGVYASYFLLPLYLEKLRKFPTIEAGEILFCPAAATGIVS- 314
GL K F V + V ++P ++ + + T E G ++ P + I+
Sbjct: 251 GLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFG 310

Query: 315 LISGILMDRKILSRKASIVIGILIFIFGTHLQSKLDLEMGKTQIILFLLPWGIGMGFFFP 374
I GIL+DR+ +I + L F L F+ I
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSF-------LTASFLLETTSWFMT-IIIVFVLGGL 362

Query: 375 ALSQVSLGNFKGEILRQASA-----LQNLLRLVGGSVGTALSTHIL 415
+ ++ + L+Q A L N + G A+ +L
Sbjct: 363 SFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0866RTXTOXIND983e-24 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 98.0 bits (244), Expect = 3e-24
Identities = 66/384 (17%), Positives = 134/384 (34%), Gaps = 48/384 (12%)

Query: 50 ASGKIVKLFKKEYESVSKEEPLFKVDDSLYRKDVEILKAKLESLKQKKKELSEKLSRLKE 109
+ + ++ KE ESV K + L K+ D ++ L + ++ ++
Sbjct: 103 ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIEL 162

Query: 110 QLPADVKISKENLKALEKKLNQLKYQELMEKTNYETSTQKAESSLKAAEK---------G 160
++K+ E + L+ L+++ QK + L +K
Sbjct: 163 NKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLAR 222

Query: 161 LEAAKVSFNHWKNQYNRYKRLYKKRIISKEQLEEVELAYKEALYKLESAKARLQAAKGDL 220
+ + K++ + + L K+ I+K + E E Y E A L+ K
Sbjct: 223 INRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVE-------AVNELRVYKS-- 273

Query: 221 ENAKSLKNRIAIIRKQQEEVKNKIEALKEQVKISKANLKKINELSHSIRQLEEDIKSIES 280
++ I + K + + + + K NE+ +RQ ++I +
Sbjct: 274 --------QLEQIESEILSAKEEYQLVTQLFK---------NEILDKLRQTTDNIGLLTL 316

Query: 281 QIEKAKILLSHTLVKSPVEGFIAK-KWKEEGDFISPGLPVYSIY-NSKSFFVLAWIEEDK 338
++ K + +++++PV + + K EG ++ + I + V A ++
Sbjct: 317 ELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKD 376

Query: 339 IKDIKVGNKVKVELEVCKKTFKGKVYSIGTSAGSIFSLIPRDTSQ----GEYTKVTQRIP 394
I I VG +++E F Y G G + I D + G V I
Sbjct: 377 IGFINVGQNAIIKVE----AFPYTRY--GYLVGKV-KNINLDAIEDQRLGLVFNVIISIE 429

Query: 395 VKIKVEKVPPVCIKPGTNVTVYIK 418
+ + G VT IK
Sbjct: 430 ENCLSTGNKNIPLSSGMAVTAEIK 453


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0877HTHFIS5100.0 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 510 bits (1314), Expect = 0.0
Identities = 172/475 (36%), Positives = 266/475 (56%), Gaps = 38/475 (8%)

Query: 5 MEKRKVLIIEDDDIQRELLKEILKESGFEVFTSSTAEKGLQTVAKNSPAVVVTDVRLPGM 64
M +L+ +DD R +L + L +G++V +S A + +A +VVTDV +P
Sbjct: 1 MTGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDE 60

Query: 65 DGLTFLKKLKQEHSEIEVIVITAFSNVEDAVSAIKAGAFHYVTKPFDPQVLINLIDKAC- 123
+ L ++K+ ++ V+V++A + A+ A + GA+ Y+ KPFD LI +I +A
Sbjct: 61 NAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALA 120

Query: 124 ----QLASLRKIPKKDGEIIYASKLMEELLEKASLFAKTEAPVLILGESGVGKELIARFI 179
+ + L + ++ S M+E+ + +T+ ++I GESG GKEL+AR +
Sbjct: 121 EPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARAL 180

Query: 180 HKESGRK-GKFVSVNCAAIPSELFESELFGYEKGAFTGALRSKPGLFEEADGGTIFLDEV 238
H R+ G FV++N AAIP +L ESELFG+EKGAFTGA G FE+A+GGT+FLDE+
Sbjct: 181 HDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEI 240

Query: 239 GELPLNLQAKFLRVLQENEVRRVGGTQTKKVDVKVVAATNRDLGELVKKGDFREDLYYRL 298
G++P++ Q + LRVLQ+ E VGG + DV++VAATN+DL + + +G FREDLYYRL
Sbjct: 241 GDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDLYYRL 300

Query: 299 NVLTLRIPPLRERPEDILELTGFFLRKFSKKYNKKVEITPEALQILLSYSFPGNVRELEN 358
NV+ LR+PPLR+R EDI +L F+++ K+ EAL+++ ++ +PGNVRELEN
Sbjct: 301 NVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNVRELEN 360

Query: 359 LIHRLVI-TSMDKIRPEDLTDLKEKE-------------------------------NHC 386
L+ RL D I E + + E +
Sbjct: 361 LVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQYFASFG 420

Query: 387 NEIDFSKPLPEKLAEFEKKMIEEALKRSDYVQVKAAKLLGIDEKSLRYKRKKYGI 441
+ + S LAE E +I AL + Q+KAA LLG++ +LR K ++ G+
Sbjct: 421 DALPPSGLYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGV 475


11Dester_1067Dester_1080Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_10672151.864034Biotin synthase
Dester_10683163.087649mutual gliding protein A
Dester_10693173.618611Roadblock/LC7 family protein
Dester_10703164.173029Pyruvate synthase
Dester_10710153.395502Pyruvate synthase
Dester_1072-2171.411446pyruvate ferredoxin/flavodoxin oxidoreductase,
Dester_10730193.185980pyruvate/ketoisovalerate oxidoreductase, gamma
Dester_10740213.487473Recombination protein recR
Dester_10750264.571026UPF0133 protein ybaB
Dester_10760274.985597transposase IS4 family protein
Dester_10770255.310089hypothetical protein
Dester_10781245.676148L-lactate permease, putative
Dester_1079-2174.301244amino acid-binding ACT domain protein
Dester_1080-2143.610779Phenylacetate--CoA ligase
12Dester_1130Dester_1137Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_11302264.189645ATP-dependent hsl protease ATP-binding subunit
Dester_11315366.273223ATP-dependent protease hslV
Dester_11326437.208119hypothetical protein
Dester_11335406.675537**Integrase catalytic region
Dester_11345437.224149ATP citrate synthase
Dester_11353396.820017ATP-grasp domain protein
Dester_11362356.412447isocitrate dehydrogenase, NADP-dependent
Dester_11370264.091482aconitate hydratase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1133HTHFIS290.028 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.028
Identities = 7/30 (23%), Positives = 12/30 (40%), Gaps = 3/30 (10%)

Query: 57 GNARKTCRYFGISPTTFYKWKKRYDKYGIE 86
GN K G++ T K+ + G+
Sbjct: 450 GNQIKAADLLGLNRNTLR---KKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1135RTXTOXINA300.018 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 30.3 bits (68), Expect = 0.018
Identities = 18/63 (28%), Positives = 31/63 (49%), Gaps = 4/63 (6%)

Query: 369 DAFKEYADKMKEVGVKI--YVRRGGPNYEVGLARIKKAAEELGLPIEVYGPETHITAIVK 426
DA K+ A++ + G ++ + + L + + A+ELG IEV E + TAI K
Sbjct: 33 DALKKAAEQTRNAGNRLILLIPKDYKGQGSSLNDLVRTADELG--IEVQYDEKNGTAITK 90

Query: 427 KAL 429
+
Sbjct: 91 QVF 93


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1136RTXTOXINA300.044 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 29.9 bits (67), Expect = 0.044
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 2/49 (4%)

Query: 249 ATMMRVSDPAIFGDAIRVYYKE--LFEKHGKELEEIGFDPNKGLIDLEN 295
A + R D + G + YY+E EK E ++ FDP KG IDL +
Sbjct: 488 AGVTRNGDKTLSGKSYIDYYEEGKRLEKKXDEFQKQVFDPLKGNIDLSD 536


13Dester_1176Dester_1189Y        NYGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1176316-2.314603hypothetical protein
Dester_1177212-1.477869UPF0173 metal-dependent hydrolase
Dester_11782160.196310Patatin
Dester_11793160.162130Peptidase M23
Dester_11802141.915731twin-arginine translocation protein, TatA/E
Dester_11811131.802216NADH-ubiquinone/plastoquinone oxidoreductase
Dester_11821131.666271NADH-quinone oxidoreductase, B subunit
Dester_11831141.210349transposase IS116/IS110/IS902 family protein
Dester_1184312-0.101297NADH dehydrogenase (ubiquinone) 30 kDa subunit
Dester_11854100.126243NADH dehydrogenase (quinone)
Dester_1186611-0.027492NADH dehydrogenase (quinone)
Dester_1187713-0.186815NADH dehydrogenase subunit I
Dester_1188614-0.426658NADH-ubiquinone/plastoquinone oxidoreductase
Dester_11892130.287717NAD(P)H-quinone oxidoreductase subunit 4L
14Dester_1222Dester_1253Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1222216-4.555613Flagellar protein FlgJ
Dester_1223216-4.998097flagellar biosynthesis protein FlhA
Dester_1224218-6.504634flagellar biosynthetic protein FlhF
Dester_1225218-6.621462cobyrinic acid ac-diamide synthase
Dester_1226419-7.062060RNA polymerase, sigma 28 subunit, FliA/WhiG
Dester_1227118-5.558526hypothetical protein
Dester_1228117-4.391577tetratricopeptide repeat domain protein
Dester_1229116-2.195844flagellar basal-body rod protein FlgB
Dester_1230017-2.150965flagellar basal-body rod protein FlgC
Dester_1231-115-1.805691Flagellar hook-basal body complex protein fliE
Dester_1232-115-1.777070flagellar M-ring protein FliF
Dester_1233116-2.381498hypothetical protein
Dester_1234216-1.715507ATPase, FliI/YscN family
Dester_1235216-2.433907flagellar hook capping protein
Dester_1236317-2.592544fagellar hook-basal body protein
Dester_1237317-5.167056flagellar basal body-associated protein FliL
Dester_1238118-4.836667surface presentation of antigens (SPOA) protein
Dester_1239116-4.761836hypothetical protein
Dester_1240016-5.336950flagellar biosynthetic protein FliP
Dester_1241016-5.381285flagellar biosynthetic protein FliQ
Dester_1242-214-4.567949flagellar biosynthetic protein FliR
Dester_1243-112-3.350564flagellar biosynthetic protein FlhB
Dester_1244211-3.016030hypothetical protein
Dester_1245213-2.179450hypothetical protein
Dester_12460140.932560type IV pilus assembly PilZ
Dester_12472171.859884tRNA-specific 2-thiouridylase mnmA
Dester_12482253.816049ATP synthase subunit b
Dester_12492253.830993ATP synthase subunit b
Dester_12501234.030224ATP synthase subunit delta
Dester_12510214.118313ATP synthase subunit alpha
Dester_12520183.407727ATP synthase gamma chain
Dester_12531214.004052ATP synthase subunit beta
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1222FLGFLGJ396e-07 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 38.9 bits (90), Expect = 6e-07
Identities = 20/94 (21%), Positives = 43/94 (45%), Gaps = 9/94 (9%)

Query: 10 WDIANIKQIK---------NESEAIKEFEAYFVRIFLKEARKSIPKGLFNTSFSANFYYD 60
WD ++ ++K N ++ E FV++ LK R ++PK +S Y
Sbjct: 13 WDAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDGLFSSEHTRLYTS 72

Query: 61 MLDMELAEVISQKDPLHLEKFLQEALSKYQKISK 94
M D ++A+ ++ L L + + + ++ Q + +
Sbjct: 73 MYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPE 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1224CHANLCOLICIN362e-04 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 36.2 bits (83), Expect = 2e-04
Identities = 25/139 (17%), Positives = 50/139 (35%), Gaps = 8/139 (5%)

Query: 17 QAKRELGEEIDILYYEVEKERSFLPFFRKKKYKLFVVPKEKRENNEIEKLEEELNEVREL 76
+EL E + L PFF + ++ + + ++ E +N +
Sbjct: 245 AKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINAD 304

Query: 77 LTNIKSSL--EENKLANSSLPIPEHIDSTSSCEENLTTEFTGDALELIKVLIQK-----G 129
+T I+ ++ N + E ++ + NL DA++ Q G
Sbjct: 305 ITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKDAVDATVSFYQTLTEKYG 364

Query: 130 VK-KNIAEELVKEACGLDI 147
K +A+EL ++ G I
Sbjct: 365 EKYSKMAQELADKSKGKKI 383


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1231FLGHOOKFLIE571e-14 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 57.4 bits (138), Expect = 1e-14
Identities = 21/81 (25%), Positives = 41/81 (50%), Gaps = 1/81 (1%)

Query: 16 KEKHNQKANGFKDLLENFIKDVNSDLKESRKAEENLISGNVQ-NIEEIMYKIEKADLSLR 74
+E Q F L + ++ +R E G + ++M ++KA +S++
Sbjct: 23 QESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQ 82

Query: 75 LLVEIRNKALESYQEIMRMQV 95
+ +++RNK + +YQE+M MQV
Sbjct: 83 MGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1232FLGMRINGFLIF357e-119 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 357 bits (917), Expect = e-119
Identities = 170/558 (30%), Positives = 290/558 (51%), Gaps = 54/558 (9%)

Query: 7 QTKVQEIFKK-NANPKNVILLLSALTLVSFLAFIAIKQSTTEDYAVLYTHLSPDDAGSIL 65
Q K E + ANP+ I L+ A + + + + T DY L+++LS D G+I+
Sbjct: 9 QPKPLEWLNRLRANPR--IPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIV 66

Query: 66 SVLQEEHIPYKVEGNGSIILVPKEKVYDIRLKLAAKGLPHGKVVGFELFDEPKLGITQFQ 125
+ L + +IPY+ I VP +KV+++RL+LA +GLP G VGFEL D+ K GI+QF
Sbjct: 67 AQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFS 126

Query: 126 ENVEFLRALEGEIERTIKRINAIQDVKVNIALPKDSIFVRESEEPKASILVNLWPGRELT 185
E V + RALEGE+ RTI+ + ++ +V++A+PK S+FVRE + P AS+ V L PGR L
Sbjct: 127 EQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALD 186

Query: 186 KEQVKAIVFLVSHAVPGLKPENVTVIDNRGRVLTDLLSGNEDETGSSKELEVKRKLEKEI 245
+ Q+ A+V LVS AV GL P NVT++D G +LT S + +L+ +E I
Sbjct: 187 EGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQ--SNTSGRDLNDAQLKFANDVESRI 244

Query: 246 ERKIQSMLSQVLGSGKVVVRASVEIETGRLEKKEELYDPDMTAVVSERKIQEKETGIK-- 303
+R+I+++LS ++G+G V + + +++ E+ EE Y P+ A + + ++ +
Sbjct: 245 QRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVG 304

Query: 304 -PKEQGVPGTTTNVP-PVLNLNQGNEILKKE----------------------KKDVTTN 339
GVPG +N P P ++ +++ T+N
Sbjct: 305 AGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSN 364

Query: 340 YDVSKTIQKTITPIFKIKRISVGVLVDGKYQKEKDKAGNEIIKFVPRSQEEIKTYEEIVK 399
Y+V +TI+ T + I+R+SV V+V+ K + K +P + +++K E++ +
Sbjct: 365 YEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADG--------KPLPLTADQMKQIEDLTR 416

Query: 400 SAIGYDPKRGDTVTVASVPFEAKQFVQKEK---SQKKFPWIYIAAGGGLLTLILVGIIAL 456
A+G+ KRGDT+ V + PF A E Q+ F +AAG LL L++ I+
Sbjct: 417 EAMGFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWR 476

Query: 457 KLLKSK--------KTEPQQPEIPETLMAEMKARAEHKEEL----EELHIESDPLYIKIV 504
K ++ + K +Q ++ + ++ R E+L + ++ + +I
Sbjct: 477 KAVRPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIR 536

Query: 505 EIAKEHPELVANVISKWI 522
E++ P +VA VI +W+
Sbjct: 537 EMSDNDPRVVALVIRQWM 554


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1236FLGHOOKAP1457e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.9 bits (106), Expect = 7e-07
Identities = 16/52 (30%), Positives = 31/52 (59%)

Query: 516 VISKVRSGMLEMSNVDIASEFINLITAQRAYQANARVITTDDQILQETMNIK 567
V++++ + +S V++ E+ NL Q+ Y ANA+V+ T + I +NI+
Sbjct: 495 VVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 33.8 bits (77), Expect = 0.002
Identities = 15/59 (25%), Positives = 30/59 (50%), Gaps = 4/59 (6%)

Query: 4 SFYTAFTGLNADKTWLSVISDNIANVNTVGFKKENAVFEDLLARSLTTFKNGAPVNQEI 62
A +GLNA + L+ S+NI++ N G+ ++ + +A++ +T G V +
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI----MAQANSTLGAGGWVGNGV 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1238FLGMOTORFLIN545e-13 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 53.8 bits (129), Expect = 5e-13
Identities = 20/73 (27%), Positives = 46/73 (63%)

Query: 8 LEDFKDVSLSISLCIGKKFLTLNKILKLKEGDLIEFDKKLEDYLDVYLNGQKFGIGELVI 67
++ D+ + +++ +G+ +T+ ++L+L +G ++ D + LD+ +NG GE+V+
Sbjct: 54 IDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVV 113

Query: 68 VNDKYSLRLVDLV 80
V DKY +R+ D++
Sbjct: 114 VADKYGVRITDII 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1240FLGBIOSNFLIP2346e-80 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 234 bits (598), Expect = 6e-80
Identities = 100/208 (48%), Positives = 151/208 (72%), Gaps = 1/208 (0%)

Query: 35 GNLDITLKILFLITILSLAPAILITVTSFTRIVIILSLLRHALGTPQTPPNQVIIALSLF 94
+ + ++ L IT L+ PAIL+ +TSFTRI+I+ LLR+ALGTP PPNQV++ L+LF
Sbjct: 36 QSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALF 95

Query: 95 LTLFTMAPTFQQIDELAIQPYINKKISDVEAIKRASEPIKNFMLRNTRKEDLKLFLDIRN 154
LT F M+P +I A QP+ +KIS EA+++ ++P++ FMLR TR+ DL LF + N
Sbjct: 96 LTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLAN 155

Query: 155 E-KPSSPQEISMLTLIPAFMVSEIRTALEVVFVIFLPFIVIDLLVASILMSMGMMMIPPM 213
P+ + M L+PA++ SE++TA ++ F IF+PF++IDL++AS+LM++GMMM+PP
Sbjct: 156 TGPLQGPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPA 215

Query: 214 MLSLPFKLILFVLSDGWELLIKSIILSY 241
++LPFKL+LFVL DGW+LL+ S+ S+
Sbjct: 216 TIALPFKLMLFVLVDGWQLLVGSLAQSF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1241TYPE3IMQPROT601e-15 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 59.8 bits (145), Expect = 1e-15
Identities = 21/77 (27%), Positives = 41/77 (53%)

Query: 3 VDQVITLGQKMLEIALLVGMPVLLTTFLVGIIISIFQAATQIHEMTLTFIPKIVAALLAL 62
+D ++ G K L + L++ + ++G+++ +FQ TQ+ E TL F K++ L L
Sbjct: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL 60

Query: 63 FIFGSWMLIKLIDYTKE 79
F+ W L+ Y ++
Sbjct: 61 FLLSGWYGEVLLSYGRQ 77


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1242TYPE3IMRPROT996e-27 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 99.0 bits (247), Expect = 6e-27
Identities = 55/251 (21%), Positives = 113/251 (45%), Gaps = 5/251 (1%)

Query: 9 TFSLFLLTFVRVASFFLAFPFISTTLIPLNIRILLILAFSFYLSQIIEPSQMIDITKIDL 68
+L+ +RV + P +S +P +++ + ++ I PS + +
Sbjct: 12 WLNLYFWPLLRVLALISTAPILSERSVPKRVKL----GLAMMITFAIAPSLPANDVPVFS 67

Query: 69 LSFFLLVIKEVLLGISFSILTTIYSSIFIHAAELISYSMGLTIVNIFDSTFGS-ISVLSR 127
L ++++L+GI+ + A E+I MGL+ D + VL+R
Sbjct: 68 FFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLAR 127

Query: 128 FFVYIFYVVFFFTDAYKIFIAAFVESFKIIPIGNFHLSDSLLYFFLKESKLIFFLSFKIA 187
+ ++F + + I+ V++F +PIG L+ + K LIF +A
Sbjct: 128 IMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLA 187

Query: 188 FPFIITLFITNLILALVNRLIPQINVFIVGLPLQIFIGLFFLSTGFSILIYSSKYLIEKL 247
P I L NL L L+NR+ PQ+++F++G PL + +G+ ++ ++ ++L ++
Sbjct: 188 LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEI 247

Query: 248 STDIINLIKIL 258
+ ++I L
Sbjct: 248 FNLLADIISEL 258


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1243TYPE3IMSPROT328e-114 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 328 bits (843), Expect = e-114
Identities = 105/341 (30%), Positives = 178/341 (52%), Gaps = 3/341 (0%)

Query: 7 KTEKATPRRRQKAKEEGQVLKSQDIPIAFTLLITSTLLYFYIPFAYKKLLQLFTFDFRTS 66
KTE+ TP++ + A+++GQV KS+++ ++ S +L + ++ +L S
Sbjct: 5 KTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAEQS 64

Query: 67 NNVNLWN-NYLVS--AKTFALLILPVFLVLFLGGIFSNIIQFGFLFSLKPLLPKLDNINP 123
+Y+V F L P+ V L I S+++Q+GFL S + + P + INP
Sbjct: 65 YLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKKINP 124

Query: 124 IKGLGRLFSLKTLFETFRNTLKLIIALAVGYFSGKYILSDFFSLSFISLNNQIILMLKYT 183
I+G R+FS+K+L E ++ LK+++ + + K L L + L+ +
Sbjct: 125 IEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQIL 184

Query: 184 LLLFFIFGLLSLPIAAADFLFRRWEYEENLKMSKEEIKEERKQYEGHPLIKSAIRRKQRE 243
L I + + I+ AD+ F ++Y + LKMSK+EIK E K+ EG P IKS R+ +E
Sbjct: 185 RQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQFHQE 244

Query: 244 IAMKRMMAEIPKADVVITNPTHYAVALRYERGKMHAPKVIAKGVDNIALKIKKIALEHNI 303
I + M + ++ VV+ NPTH A+ + Y+RG+ P V K D ++KIA E +
Sbjct: 245 IQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGV 304

Query: 304 PIEENPYLARVLYESCDIGSFIPEEFYQAIAKILAKVYKKK 344
PI + LAR LY + +IP E +A A++L + ++
Sbjct: 305 PILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQN 345


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1244IGASERPTASE270.031 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 27.3 bits (60), Expect = 0.031
Identities = 14/75 (18%), Positives = 29/75 (38%), Gaps = 2/75 (2%)

Query: 21 QEIEKHEAQKEIERLKKLKVEVQRLLEEKKNLLKKIEEEKKQLEEEKKAFEKKIKEIESE 80
++ + E+ + E Q E K +EEK ++E EK K+ S
Sbjct: 1074 SNVKANTQTNEVAQSGSETKETQTT--ETKETATVEKEEKAKVETEKTQEVPKVTSQVSP 1131

Query: 81 RYKKLAQIFEKMDPE 95
+ ++ + + +P
Sbjct: 1132 KQEQSETVQPQAEPA 1146


15Dester_1287Dester_1294Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1287018-4.113016riboflavin biosynthesis protein RibD
Dester_1288020-5.384113Methyltransferase type 12
Dester_1289017-4.655726glycosyl transferase family 2
Dester_1290118-4.935831glycosyl transferase family 2
Dester_1291216-3.252010glycosyl transferase family 2
Dester_1292315-3.046632hypothetical protein
Dester_1293514-3.064103glycosyl transferase family 2
Dester_1294713-2.023348flagellin domain protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1294FLAGELLIN1203e-33 Flagellin signature.
		>FLAGELLIN#Flagellin signature.

Length = 507

Score = 120 bits (302), Expect = 3e-33
Identities = 59/233 (25%), Positives = 105/233 (45%), Gaps = 7/233 (3%)

Query: 2 ALRINYNYQSDFTHFNLLQTEANMNKSLERLATGYRINRAADDAAGLYIADQLKTYAVSL 61
A IN N S T NL +++++++ ++ERL++G RIN A DDAAG IA++ + L
Sbjct: 1 AQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDDAAGQAIANRFTSNIKGL 60

Query: 62 EQATRNAHDGISIAQIAQSALTNVYNILNDIKAKAIEAANDSQDSATRQIIQQDINKLVD 121
QA+RNA+DGISIAQ + AL + N L ++ +++A N + + + IQ +I + ++
Sbjct: 61 TQASRNANDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLE 120

Query: 122 VIGKIFTDTEFNGINVFSSGTAVTFTIHYGGRSGQELLMQGATASAAAAADTSAPSTITL 181
I ++ T+FNG+ V S + G G+ + + +
Sbjct: 121 EIDRVSNQTQFNGVKVLSQDNQMKI--QVGANDGETITIDLQKIDVKSLGLDG-----FN 173

Query: 182 GNTGYTLDVTSQTKAEATISTVDALIKEVDKLNAKYGSYQIELEKLITNNESQ 234
N V + ++ D +K S + + +
Sbjct: 174 VNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDK 226



Score = 100 bits (249), Expect = 5e-26
Identities = 56/286 (19%), Positives = 101/286 (35%), Gaps = 5/286 (1%)

Query: 5 INYNYQSDFTHFNLLQTEANMNKSLERLATGYRINRAADDAAGLYIADQLKTYAVSLEQA 64
+ + + L +A N +++ T A+ A K +
Sbjct: 223 VPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKG 282

Query: 65 TRNAHDGISIAQIAQSALTNVYNILNDIKAKAIEAANDSQDSATRQIIQQ----DINKLV 120
D + T + + I A + D+AT Q + +N
Sbjct: 283 VTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQF 342

Query: 121 DVIGKIFTDTEFNGINVFSSGTAVTFTIHYGGRSGQELLMQGATASAAAAADTSAPSTIT 180
K ++ ++ I G A +
Sbjct: 343 TFDDKTKNESAKLSDLEANNAVKGESKITVNGAEYTANAAGDKVTLAGKTM-FIDKTASG 401

Query: 181 LGNTGYTLDVTSQTKAEATISTVDALIKEVDKLNAKYGSYQIELEKLITNNESQRINSQE 240
+ ++ ++++D+ + +VD + + G+ Q + ITN + N
Sbjct: 402 VSTLINEDAAAAKKSTANPLASIDSALSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNS 461

Query: 241 AESRIRNVDFAKEMSEFTRNQILMQSGTAMLAQANQLPQLVLQLLR 286
A SRI + D+A E+S ++ QIL Q+GT++LAQANQ+PQ VL LLR
Sbjct: 462 ARSRIEDADYATEVSNMSKAQILQQAGTSVLAQANQVPQNVLSLLR 507


16Dester_1310Dester_1317Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_13102210.177795glycosyl transferase group 1
Dester_13114222.332147transposase IS4 family protein
Dester_13125232.439102GDP-mannose 4,6-dehydratase
Dester_13136242.152742Methyltransferase type 11
Dester_13147232.521441glycosyl transferase group 1
Dester_13159182.394329acyltransferase 3
Dester_13168161.118483hypothetical protein
Dester_1317112-3.309568hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1312NUCEPIMERASE968e-25 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 96.4 bits (240), Expect = 8e-25
Identities = 68/347 (19%), Positives = 128/347 (36%), Gaps = 49/347 (14%)

Query: 3 KALITGIRGQDGAYLAKLLLEKGYEVYGADRRSGDSSNWRLKELGIE----KDVKVVYMD 58
K L+TG G G +++K LLE G++V G D + D + LK+ +E + +D
Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLN-DYYDVSLKQARLELLAQPGFQFHKID 60

Query: 59 LLELTNIMRVIEKIQPDEVYNLAAQSFVGVSFEQPILTAEIDAMGVLKLLEAIRTLKPDT 118
L + + + + V+ + V S E P A+ + G L +LE R K
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 119 KFYQASTSEMFGKVQEIPQTEKTPF-YPRSPYGVAKLFGHWITVNYRESFNIFACSGILF 177
Y AS+S ++G +++P + +P S Y K + Y + +
Sbjct: 121 LLY-ASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGL-------- 171

Query: 178 NHESPLRGIEFVTRKITYSLARIKYGLQDKLIL--------GNLDAKRDWGYAPEYVEGM 229
P G+ F T + + K +L KRD+ Y + E +
Sbjct: 172 ----PATGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAI 227

Query: 230 WLMLQQEKPDDYVLATGETHTVREFVEKAAEVAGFSLEWEGEG--------VDT--KGID 279
+ V+ +T E AA +A + + G + +
Sbjct: 228 IRLQD-------VIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALG 280

Query: 280 RKSGKVIVEVSPEFYRPAEVDILIGNPEKAKKKLGWEPRTKFSQLVE 326
++ K ++ + +P +V + + + +G+ P T V+
Sbjct: 281 IEAKKNMLPL-----QPGDVLETSADTKALYEVIGFTPETTVKDGVK 322


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1316RTXTOXINA414e-05 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 40.7 bits (95), Expect = 4e-05
Identities = 28/132 (21%), Positives = 53/132 (40%), Gaps = 10/132 (7%)

Query: 441 GYASDVITGNSAGTTILLGSGNDNLQFVDGANAGESNIIKLGAGDDKL-------VVTTN 493
G +D + G + + G G+D Q + A N++ G G+DKL ++
Sbjct: 779 GDGNDKLIGVAGNNYLNGGDGDDEFQVQGNSLA--KNVLFGGKGNDKLYGSEGADLLDGG 836

Query: 494 NGYTYVFGEDGNDDVVINAIDTNDLV-DLGAGTDTLTLDNTNASTAVLRGVENLVIKARD 552
G + G GND + + ++ D G D L+L + + + N +I +
Sbjct: 837 EGDDLLKGGYGNDIYRYLSGYGHHIIDDDGGKEDKLSLADIDFRDVAFKREGNDLIMYKG 896

Query: 553 NGQAVTINAADS 564
G ++I +
Sbjct: 897 EGNVLSIGHKNG 908



Score = 32.2 bits (73), Expect = 0.016
Identities = 44/224 (19%), Positives = 70/224 (31%), Gaps = 23/224 (10%)

Query: 961 VKTATIALGGGTDKLDL--TNLANLDANGVGAVINLSSFTQTVDGVSVDAGKIVEFDGTD 1018
VK +++G T+K +++ + NL S + + D +F
Sbjct: 681 VKEQEVSVGKRTEKTQYRSYEFTHINGKNLTETDNLYSVEELIGTTRADKFFGSKFTDIF 740

Query: 1019 DGTNNITDGYTITVNDVEEIVGTKGADIIFAANTGTTITSEAGADKIVLGAGADKVIIAA 1078
G + + I G G D ++ T++ G D++ G G DK+I
Sbjct: 741 HGADG-----------DDLIEGNDGNDRLYGDKGNDTLSGGNGDDQLYGGDGNDKLI--- 786

Query: 1079 AGETGTITVADKDSSGDLSDGDTISGSFDVISNFEHGTDKLDISAVNDGTSTDTW-DSTN 1137
G G + D + + + G DKL S D D
Sbjct: 787 -GVAGNNYLNGGDGDDEFQVQG--NSLAKNVLFGGKGNDKLYGSEGADLLDGGEGDDLLK 843

Query: 1138 GLDANAYQIVLGTWDETTNTFTVDSGSGTDTMVLFDDGLSDVAV 1181
G N L + D G D + L D DVA
Sbjct: 844 GGYGNDIYRYLSGYGHHI---IDDDGGKEDKLSLADIDFRDVAF 884


17Dester_1355Dester_1371Y        Y        NPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1355219-3.125130hypothetical protein
Dester_1356418-3.936522hypothetical protein
Dester_1357419-4.480805DNA polymerase beta domain protein region
Dester_1358419-4.388957Undecaprenyl-phosphate galactose
Dester_1359821-5.010496polysaccharide biosynthesis protein
Dester_1360619-4.201310UDP-galactopyranose mutase
Dester_1361420-3.479645hypothetical protein
Dester_1362420-2.751613hypothetical protein
Dester_1363419-2.539030Uncharacterized protein family UPF0150
Dester_1364419-2.712666glycosyl transferase family 2
Dester_1365316-1.574947glycosyl transferase group 1
Dester_1366519-0.375906mannose-1-phosphate
Dester_13677221.015329hypothetical protein
Dester_13685201.247618hypothetical protein
Dester_13695201.584902hypothetical protein
Dester_13703202.018606hypothetical protein
Dester_13713201.790427glucose-1-phosphate thymidylyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1361ANTHRAXTOXNA320.004 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 32.0 bits (72), Expect = 0.004
Identities = 19/73 (26%), Positives = 31/73 (42%), Gaps = 15/73 (20%)

Query: 289 YFEDVPISEEYKFYDKEENIPKIINKIKDCFENFEERYKDFNYYREV-----------IK 337
E VP + + ++K+ PK+I IKD N E + Y E+ K
Sbjct: 131 RGEKVPFASRF-VFEKKRETPKLIINIKDYAINSE---QSKEVYYEIGKGISLDIISKDK 186

Query: 338 NELQKFLEDLKSI 350
+ +FL +KS+
Sbjct: 187 SLDPEFLNLIKSL 199


18Dester_1385Dester_1395Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_138527-1.355222Glycyl-tRNA synthetase beta subunit
Dester_138617-1.763286Glycyl-tRNA synthetase alpha subunit
Dester_138729-2.750566exsB protein
Dester_1388210-2.627498oxygen-independent coproporphyrinogen III
Dester_1389211-1.748961Tetratricopeptide TPR_1 repeat-containing
Dester_1390112-0.407319transposase, IS605 OrfB family
Dester_13912200.409759hypothetical protein
Dester_13923201.934898hypothetical protein
Dester_13933172.020942Ornithine carbamoyltransferase
Dester_13943191.95199630S ribosomal protein S20
Dester_13952181.342821protein of unknown function DUF125
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1391BCTERIALGSPH358e-05 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 34.5 bits (79), Expect = 8e-05
Identities = 13/52 (25%), Positives = 29/52 (55%), Gaps = 4/52 (7%)

Query: 2 KKAFTLLELVIVLLIVSLTLTITLPTFF----NIEATSMENFENKLKTATNS 49
++ FTLLE++++LL++ ++ + L F + A ++ FE +L+
Sbjct: 3 QRGFTLLEMMLILLLMGVSAGMVLLAFPASRDDSAAQTLARFEAQLRFVQQR 54


19Dester_1433Dester_1452Y        Y        YPathogenicity Island (biased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1433213-1.969297Peptidase M23
Dester_1434214-2.648948*hypothetical protein
Dester_1435112-2.568459primosomal protein N'
Dester_1436-114-1.737043hypothetical protein
Dester_1437-114-1.438186glycosyl transferase group 1
Dester_1438-113-1.224070glycosyl transferase family 2
Dester_1439112-0.781754polysaccharide deacetylase
Dester_1440312-0.6989194-diphosphocytidyl-2-C-methyl-D-
Dester_1441113-1.237250*****hypothetical protein
Dester_1442114-1.741082N-methylation domain-containing protein
Dester_1443013-2.160889type II secretion system protein E
Dester_1444216-3.086815hypothetical protein
Dester_1445-118-4.202414hypothetical protein
Dester_1446-117-3.743381Tetratricopeptide TPR_1 repeat-containing
Dester_1447212-3.177660type II and III secretion system protein
Dester_1448212-3.223323hypothetical protein
Dester_1449210-2.113373hypothetical protein
Dester_1450210-1.438041hypothetical protein
Dester_1451210-1.036013Type II secretion system F domain
Dester_145239-1.034085chromosome segregation protein SMC
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1433RTXTOXIND320.003 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 31.7 bits (72), Expect = 0.003
Identities = 11/50 (22%), Positives = 23/50 (46%), Gaps = 11/50 (22%)

Query: 160 ASLSGRVVLARDFYYTGNTIVIDHGLGIYTLYAHLSKILVKEGQIVQAGQ 209
A+ +G++ + G + I + + +I+VKEG+ V+ G
Sbjct: 84 ATANGKLTHS------GRSKEIKPIEN-----SIVKEIIVKEGESVRKGD 122


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1442BCTERIALGSPG413e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 41.4 bits (97), Expect = 3e-07
Identities = 34/137 (24%), Positives = 57/137 (41%), Gaps = 33/137 (24%)

Query: 1 MKERKGFTLVELAIVLVIIGLLLGAV---LKGQELIQNAKYKKLINDLQGLSAAVYTYY- 56
+++GFTL+E+ +V+VIIG+L V L G + + A +K ++D+ L A+ Y
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNK--EKADKQKAVSDIVALENALDMYKL 61

Query: 57 --DRY----------------KALPGDDPKAG-------DKWGSTYSNIINGDGNG--LI 89
Y L + K G D WG+ Y + G+ L+
Sbjct: 62 DNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLL 121

Query: 90 SGSPTSTTNTDESVQIW 106
S P T++ + W
Sbjct: 122 SAGPDGEMGTEDDITNW 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1444BCTERIALGSPH369e-05 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 36.1 bits (83), Expect = 9e-05
Identities = 11/29 (37%), Positives = 21/29 (72%)

Query: 1 MKRSGFTLIEMAIILVILGLILGIGMGTM 29
M++ GFTL+EM +IL+++G+ G+ +
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAF 29


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1445BCTERIALGSPG342e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 34.5 bits (79), Expect = 2e-04
Identities = 12/36 (33%), Positives = 23/36 (63%)

Query: 17 RKGFSLIEIAIVLVIVSILLGLGIRSCISGIETAKI 52
++GF+L+EI +V+VI+ +L L + + + E A
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADK 42


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1446SYCDCHAPRONE412e-06 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 40.7 bits (95), Expect = 2e-06
Identities = 25/129 (19%), Positives = 44/129 (34%)

Query: 206 LKIDPDYAEAYAGIGFLYLKLNSPKAAVIAFRRAHSLNPKEISYSVNLAISLLGSGNIDE 265
+I D E + F + + A F+ L+ + + + L G D
Sbjct: 29 NEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDL 88

Query: 266 AILEFQKLKNKYPFLPEIYYNEAVAYLKKGYYKKAIEDFEIFLELTKANKFYEDYREEVL 325
AI + P ++ A L+KG +A + EL +++ V
Sbjct: 89 AIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRVS 148

Query: 326 KVLNQIKLI 334
+L IKL
Sbjct: 149 SMLEAIKLK 157



Score = 36.4 bits (84), Expect = 5e-05
Identities = 18/86 (20%), Positives = 35/86 (40%), Gaps = 1/86 (1%)

Query: 181 LYTYLGYAYTHLGRYTKALNAFKKALKIDPDYAEAYAGIGFLYLKLNSPKAAVIAFRRAH 240
LY+ + G+Y A F+ +D + + G+G + A+ ++
Sbjct: 39 LYSL-AFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGA 97

Query: 241 SLNPKEISYSVNLAISLLGSGNIDEA 266
++ KE + + A LL G + EA
Sbjct: 98 IMDIKEPRFPFHAAECLLQKGELAEA 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1447BCTERIALGSPD1614e-45 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 161 bits (409), Expect = 4e-45
Identities = 84/309 (27%), Positives = 139/309 (44%), Gaps = 39/309 (12%)

Query: 211 DKTASYSVEPISGTVIVTAKPETLKKVKEFIDTINGISDRQVLIEAKIVEVKLDKRNELG 270
DK + +IVTA P+ + ++ I ++ I QVL+EA I EV+ LG
Sbjct: 307 DKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLD-IRRPQVLVEAIIAEVQDADGLNLG 365

Query: 271 INW--KYLTFSNFLGSGGEYNTI---------------SFNSGAPEGKPFQLSIVKVNNT 313
I W K + F SG +T S S + N
Sbjct: 366 IQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGN-- 423

Query: 314 FSGLLGILSQFGKVNVLSSPRILAMNGQPAMIKVGRDYLAIYRTQTTSTTSTSSQTATTL 373
++ LL LS K ++L++P I+ ++ A VG++ T S T++ T+
Sbjct: 424 WAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEV----PVLTGSQTTSGDNIFNTV 479

Query: 374 TTEEVTTNSILTEGVVLTIIPKIDDKGNIILNISPAISSLDSPLITGSTGETTDFINKVY 433
+ V G+ L + P+I++ +++L I +SS + + T+ +
Sbjct: 480 ERKTV--------GIKLKVKPQINEGDSVLLEIEQEVSS-----VADAASSTSSDLGAT- 525

Query: 434 SVNIRQLNTVVRVKNGQTVILGGLIAKSKSKEKEGVPILQDIPLLGNAFKSTSTISSKTE 493
N R +N V V +G+TV++GGL+ KS S + VP+L DIP++G F+STS SK
Sbjct: 526 -FNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRN 584

Query: 494 LVIMLTPYV 502
L++ + P V
Sbjct: 585 LMLFIRPTV 593



Score = 43.8 bits (103), Expect = 1e-06
Identities = 27/182 (14%), Positives = 69/182 (37%), Gaps = 22/182 (12%)

Query: 77 SFDNIDLKKALLALGKATGYNVIVPPDIEGKVSIE----LKGESLKDSLNSLLKPFGYSY 132
SF D+++ + + K VI+ P + G +++ L E S+L +G++
Sbjct: 33 SFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVLDVYGFAV 92

Query: 133 KIDAKNIYVISKETKVFHINLPQTKRQFSSSIEASIGGSSEGTGSTTTTSTATMSIGNSY 192
+ + + +K ++++ + ++ G G T ++ +
Sbjct: 93 INMNNGVLKVVR-----------SKDAKTAAVPVA-SDAAPGIGDEVVTRVVPLTNVAAR 140

Query: 193 DLDIWNNIKSSIDVIVKNDKTASYSVEPISGTVIVTAKPETLKKVKEFIDTINGISDRQV 252
DL + + N S S +++T + +K++ ++ ++ DR V
Sbjct: 141 DL------APLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSV 194

Query: 253 LI 254
+
Sbjct: 195 VT 196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1451BCTERIALGSPF1863e-57 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 186 bits (475), Expect = 3e-57
Identities = 93/405 (22%), Positives = 186/405 (45%), Gaps = 5/405 (1%)

Query: 1 MARYKVTFLSQDGIVNTEIVEAKNESELFSLFSERDVILLEYKKDWFSFLKEFSLLDLFQ 60
MA+Y L G EA + + L ER ++ L ++ K S +
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 61 R-RKISKQELADFCFYFGRALDMGISVLEILEDIGKSSKNKYFRKVMETLRERVTAGSSL 119
R ++S +LA + + + E L+ + K S+ + ++M +R +V G SL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 120 SEAME-LTGAFPPELIGLVKVGESTDALPKVFLNYAEYLDWVISIEKEVKQALSYPIFVS 178
++AM+ G+F +V GE++ L V A+Y + + ++QA+ YP ++
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 179 FIMVFTIAIMFGYIIPQIIPAITAMGLKEYPLPTKILLWSGKYVQIFWKEIVITPILLVI 238
+ + ++I+ ++P+++ M + PL T++L+ V+ F +++ + +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMK-QALPLSTRVLMGMSDAVRTFGPWMLLALLAGFM 239

Query: 239 FFKLIMRYSIKARYIWDRLKISFPLIGDIFQKASLSRDMRALAEVYRSGGTILRALDIII 298
F++++R K R + R + PLIG I + + +R R L+ + S +L+A+ I
Sbjct: 240 AFRVMLRQE-KRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISG 298

Query: 299 NHVEQNLYIKSIFQKVKENIMVGDMLSVAMERSGFFQSTIIRMIKLGEETGALDKSLLRL 358
+ V N Y + + + G L A+E++ F + MI GE +G LD L R
Sbjct: 299 D-VMSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERA 357

Query: 359 AEIYEDDMRRKIQTMTVVIEPTLQLVLGGILGIVALGILLPVYNI 403
A+ + + ++ + EP L + + ++ + L IL P+ +
Sbjct: 358 ADNQDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQL 402


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1452GPOSANCHOR642e-12 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 63.9 bits (155), Expect = 2e-12
Identities = 42/296 (14%), Positives = 106/296 (35%)

Query: 202 RTLKSQAEKAKKFQELRNLEKELELKLLGLQLKNLRLEKEVSENSLKLLQEDRISLEREV 261
+ + R + E+E L L+ +L + ++ L E+ + + ++
Sbjct: 42 AVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKL 101

Query: 262 SVLQVELEELRKELESITKEIEETSQELYEVEKSKKEASVKREFLQKEIKRLEEELKEKT 321
L E +++ + + + L S K + L+ E L +
Sbjct: 102 RKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLE 161

Query: 322 FEREHKIRKIESIRKELQLIFQEESELQKKLEDLENREKEKERIVKELQQKRKAFEERLK 381
E + + +++ + E++ L+ + +LE + K K E
Sbjct: 162 KALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKA 221

Query: 382 ELKSSLSTTSTQISKLQLDMAREEERFKSLKNIKEKLPGEIEKLQKEKEYYLSYIERFVE 441
L + + + + + K+L+ K L +L+K E +++
Sbjct: 222 ALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSA 281

Query: 442 KENSLKEKIEKLKEELENLKKEKKELLERLEVINSEVSEKREEIVSLRSKIESIEK 497
K +L+ + L+ E +L+ + + L + + ++ RE L ++ + +E+
Sbjct: 282 KIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEE 337



Score = 59.7 bits (144), Expect = 5e-11
Identities = 44/326 (13%), Positives = 103/326 (31%), Gaps = 14/326 (4%)

Query: 168 SFKEKKEETLQKLSEAEQNLESVRSVIDEVGKNLRTLKSQAEKAKKFQELRNLEKELELK 227
+ +TL+K+ E E + + +L + + +L+
Sbjct: 43 VATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLR 102

Query: 228 LLGLQLKNLRLEKEVSENSLKLLQEDRISLEREVSVLQVELEELRKELESITKEIEETSQ 287
L + + E L++ + +++ L E ++ + +
Sbjct: 103 KNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEK 162

Query: 288 ELYEVEKSKKEASVKREFLQKEIKRLEEELKEKTFEREHKIRKIESIRKELQLIFQEESE 347
L S K + L+ E LE E + K L+ +
Sbjct: 163 ALEGAMNFSTADSAKIKTLEAEKAALEARQAE--------------LEKALEGAMNFSTA 208

Query: 348 LQKKLEDLENREKEKERIVKELQQKRKAFEERLKELKSSLSTTSTQISKLQLDMAREEER 407
K++ LE + +L++ + + + T + + L+ A E+
Sbjct: 209 DSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 268

Query: 408 FKSLKNIKEKLPGEIEKLQKEKEYYLSYIERFVEKENSLKEKIEKLKEELENLKKEKKEL 467
+ N +I+ L+ EK + + L + L+ +L+ ++ KK+L
Sbjct: 269 LEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQL 328

Query: 468 LERLEVINSEVSEKREEIVSLRSKIE 493
+ + + SLR ++
Sbjct: 329 EAEHQKLEEQNKISEASRQSLRRDLD 354



Score = 57.8 bits (139), Expect = 2e-10
Identities = 64/366 (17%), Positives = 130/366 (35%), Gaps = 15/366 (4%)

Query: 655 KNSLLEMEKELENLKEKLTREEKVLSCLQSKVLPIREEIDEKEEGIDSLKEAIQQKKMEL 714
SL E +++ L+ + EK L + +I E +L + L
Sbjct: 105 DKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKAL 164

Query: 715 FEVGSKIKEARRKLADLERKENELEDKLKRAVESINSYNSRKDIFLQKIESLSKKKDELV 774
+ K+ LE ++ LE + +++ + KI++L +K L
Sbjct: 165 EGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALA 224

Query: 775 KEIEKLEEDIKKLEANIGIEKEELSKYVSKKILLAEKLKNLKERKESKERFVRTLQKEIE 834
LE+ ++ + ++ ++K L + L++ E F +I+
Sbjct: 225 ARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIK 284

Query: 835 EIEKRIEKDEENLKKAAIGVKRAEEILGGVDESIDEIKKELQLLEERRGEITSMVKTKEE 894
+E E E ++ + ++++L E + ++ + + EE
Sbjct: 285 TLEAEKAALEAEKAD-------LEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEE 337

Query: 895 ALKSKNKDLSEVQNKLKETEVAVARFNVKEEEIISKILELEKSVSDALEAALAA---GSE 951
K ++ L + A + + ++ LE + +S+A +L S
Sbjct: 338 QNKISEASRQSLRRDLDASREAKKQLEAEHQK-----LEEQNKISEASRQSLRRDLDASR 392

Query: 952 EEVKKELINLKEKISKIGNVNFLAIEEYEKVKERYGFILEQEKDLIESIKNLREAIRKLD 1011
E K+ L+E SK+ + L E E K E + L K L+E + K
Sbjct: 393 EAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQA 452

Query: 1012 EEIEKK 1017
EE+ K
Sbjct: 453 EELAKL 458



Score = 52.0 bits (124), Expect = 1e-08
Identities = 43/285 (15%), Positives = 98/285 (34%), Gaps = 14/285 (4%)

Query: 644 TGTGSIVGKFKKNSLLEMEKELENLKEKLTREEKVLSCLQSKVLPIREEIDEKEEGIDSL 703
T + + + + + E E LK K + L+ + EE+ +E +
Sbjct: 45 TRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKN 104

Query: 704 KEAIQQKKMELFEVGSKIKEARRKLADLERKENELEDKLKRAVESINSYNSRKDIFLQKI 763
+++ +K ++ E+ ++ + + L K+K + +RK + +
Sbjct: 105 DKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKAL 164

Query: 764 ESLSKKKDELVKEIEKLEEDIKKLEANIGI--------------EKEELSKYVSKKILLA 809
E +I+ LE + LEA + ++ ++K LA
Sbjct: 165 EGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALA 224

Query: 810 EKLKNLKERKESKERFVRTLQKEIEEIEKRIEKDEENLKKAAIGVKRAEEILGGVDESID 869
+ +L++ E F +I+ +E E + ++ A I
Sbjct: 225 ARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIK 284

Query: 870 EIKKELQLLEERRGEITSMVKTKEEALKSKNKDLSEVQNKLKETE 914
++ E LE + ++ + +S +DL + K+ E
Sbjct: 285 TLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLE 329



Score = 47.8 bits (113), Expect = 2e-07
Identities = 54/351 (15%), Positives = 119/351 (33%), Gaps = 17/351 (4%)

Query: 144 QIDRVLKMKPQERRLLIDEAAGITSFKEKKEETLQKLSEAEQNLESVRSVIDEVGKNLRT 203
+++ L+ + + + K L +A + + + K L
Sbjct: 124 DLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEA 183

Query: 204 LKSQAEKAKKFQELRNLEKELELKLLGLQLKNLRLEKEVSENSLKLLQEDRISLEREVSV 263
K+ E + E ++K L EK L++ +
Sbjct: 184 EKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTA 243

Query: 264 LQVELEELRKELESIT-------KEIEETSQELYEVEKSKKEASVKREFLQKEIKRLEEE 316
+++ L E ++ K +E K ++ L+ E LE +
Sbjct: 244 DSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQ 303

Query: 317 LKEKTFEREHKIRKIESIRKELQLIFQEESELQKKLEDLENREKEKERIVKELQQKRKAF 376
+ R+ R +++ R+ + + E +L+++ + E + R + ++ +K
Sbjct: 304 SQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL 363

Query: 377 EERLKELKSSLSTTSTQISKLQLDMAREEERFKSLKNIKEKLPGEIEKLQKEKEYYLSYI 436
E ++L+ + L+ D+ E K ++ E+ ++ L+K
Sbjct: 364 EAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLN------- 416

Query: 437 ERFVEKENSLKEKIEKLKEELENLKKEKKELLERLEVINSEVSEKREEIVS 487
E E S K ++ E L+ E K L E+L E+++ R S
Sbjct: 417 ---KELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQAEELAKLRAGKAS 464



Score = 47.8 bits (113), Expect = 2e-07
Identities = 46/284 (16%), Positives = 108/284 (38%)

Query: 654 KKNSLLEMEKELENLKEKLTREEKVLSCLQSKVLPIREEIDEKEEGIDSLKEAIQQKKME 713
+ +E E L + EK L + +I E +L+ + +
Sbjct: 139 DSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 198

Query: 714 LFEVGSKIKEARRKLADLERKENELEDKLKRAVESINSYNSRKDIFLQKIESLSKKKDEL 773
L + K+ LE ++ L + +++ + KI++L +K L
Sbjct: 199 LEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 258

Query: 774 VKEIEKLEEDIKKLEANIGIEKEELSKYVSKKILLAEKLKNLKERKESKERFVRTLQKEI 833
+LE+ ++ + ++ ++K L + +L+ + + ++L++++
Sbjct: 259 EARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL 318

Query: 834 EEIEKRIEKDEENLKKAAIGVKRAEEILGGVDESIDEIKKELQLLEERRGEITSMVKTKE 893
+ + ++ E +K K +E + +D ++ + LE ++ K E
Sbjct: 319 DASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISE 378

Query: 894 EALKSKNKDLSEVQNKLKETEVAVARFNVKEEEIISKILELEKS 937
+ +S +DL + K+ E A+ N K + ELE+S
Sbjct: 379 ASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEES 422


20Dester_1498Dester_1507Y        NNGenomic Island
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1498319-3.255011inorganic polyphosphate/ATP-NAD kinase
Dester_1499117-3.245706NHL repeat containing protein
Dester_1500117-3.059493UPF0042 nucleotide-binding protein yhbJ
Dester_1501-115-3.263696Miro domain protein
Dester_1502-215-2.894508hypothetical protein
Dester_1503-114-2.623003hypothetical protein
Dester_1504114-1.631253coenzyme F420-dependent
Dester_1505214-2.432341Roadblock/LC7 family protein
Dester_1506215-2.349110Radical SAM domain protein
Dester_1507216-2.087960protein of unknown function DUF1188
21Dester_0041Dester_0056N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0041311-0.588385methyl-accepting chemotaxis sensory transducer
Dester_0042210-0.329654GTP-binding protein lepA
Dester_0044210-0.250003diguanylate cyclase
Dester_0045211-0.142398response regulator receiver protein
Dester_00462100.128706response regulator receiver modulated CheW
Dester_0047213-0.051223methyl-accepting chemotaxis sensory transducer
Dester_0048215-0.030326CheW protein
Dester_00493120.003972CheA signal transduction histidine kinase
Dester_0050211-0.334035putative myosin-2 heavy chain, non muscle
Dester_00511110.490679hypothetical protein
Dester_00520100.395392transposase IS4 family protein
Dester_0053-111-0.609400Like-Sm ribonucleoprotein core
Dester_0054-111-0.324578RNA chaperone Hfq
Dester_0055011-0.517505acetyl-CoA carboxylase, biotin carboxylase
Dester_0056112-1.343520acetyl-CoA carboxylase, biotin carboxyl carrier
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0041ANTHRAXTOXNA290.017 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 29.3 bits (65), Expect = 0.017
Identities = 41/184 (22%), Positives = 71/184 (38%), Gaps = 29/184 (15%)

Query: 25 KRMAETIESLVKEIEKVFLKNNSVIAKDVES--LKKISDDLKLFLEDFIPLMRELVKVSV 82
K E + + + K N ++ LKKI D+ LE + L E+ +
Sbjct: 53 KTEKEKFKDSINNLVKTEFTNETLDKIQQTQDLLKKIPKDV---LEIYSELGGEIYFTDI 109

Query: 83 DF-KHL-YESLDAMRKSLEDI--EKIASHTELIAINASIEAARAGEAGRNFAVVANEIRT 138
D +H + L K+ + EK+ + + E R + I+
Sbjct: 110 DLVEHKELQDLSEEEKNSMNSRGEKVPFASRFVF-----------EKKRETPKLIINIKD 158

Query: 139 MARDTFKSVGEVKEIEKEIDEKISRLRNSIDTIDKIKEDVDKLVSGINSIVSISDELDLI 198
A ++ +S KE+ EI + IS D I K K + ++ I S+ SD DL+
Sbjct: 159 YAINSEQS----KEVYYEIGKGISL-----DIISKDKSLDPEFLNLIKSLSDDSDSSDLL 209

Query: 199 YRQQ 202
+ Q+
Sbjct: 210 FSQK 213


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0042TCRTETOQM1856e-53 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 185 bits (471), Expect = 6e-53
Identities = 124/509 (24%), Positives = 202/509 (39%), Gaps = 111/509 (21%)

Query: 6 IRNFCIIAHIDHGKSTLADRLLEFTGTVSK---RELKEQMLDTLELERERGITIKLNAVR 62
I N ++AH+D GK+TL + LL +G +++ + D LER+RGITI+
Sbjct: 3 IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS 62

Query: 63 MNYEASDGKTYTMHLIDTPGHVDFTYEVSRSLSACEGALLVIDATQGIEAQTIANFFLAL 122
+E + K +++IDTPGH+DF EV RSLS +GA+L+I A G++AQT F
Sbjct: 63 FQWE--NTK---VNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALR 117

Query: 123 DAGLEIIPVINKIDLPSANVEWVKEQIADVLG---------------------------- 154
G+ I INKID ++ V + I + L
Sbjct: 118 KMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDT 177

Query: 155 -LDPDDAILA--------------------------------SAKEGIGIKEILEAIVKK 181
++ +D +L SAK IGI ++E I K
Sbjct: 178 VIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNK 237

Query: 182 VPPPSGDVNKPLKALIFDSFYDNYKGVIPFIRVYDGEIKPGMRIKLMSNNKEFEVVEVGT 241
+ L +F Y + + +IR+Y G + + +S ++ ++ E+ T
Sbjct: 238 FYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSV-RISEKEKIKITEMYT 296

Query: 242 QSPN-MIKLDSLKAGEVGWLAANIKNIEDTQVGDTITNAENPTKEPCPGFRPAKPMVFAG 300
+ K+D +GE+ L + +GDT P +E P++
Sbjct: 297 SINGELCKIDKAYSGEIVILQNEFLKLNSV-LGDTKL---LPQRERIEN---PLPLLQTT 349

Query: 301 LYPIDSDRYEDLKEALEKLKLNDAALFFE-PETSAALGFGFRCGFLGLLHMEVIKERLER 359
+ P + E L +AL ++ +D L + + + FLG + MEV L+
Sbjct: 350 VEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIIL----SFLGKVQMEVTCALLQE 405

Query: 360 EFGLELIATAPSVVYKVYLSDGTVIDVQNPAEMPPK--EKIERIEEPYISASIITPAEYV 417
++ +E+ P+V+Y E P K E IE P P +
Sbjct: 406 KYHVEIEIKEPTVIYM---------------ERPLKKAEYTIHIEVP--------PNPFW 442

Query: 418 GSIMQLCQDRRGIQTGFTYLDENRVELRY 446
SI L + +G Y E+ V L Y
Sbjct: 443 ASIG-LSVSPLPLGSGMQY--ESSVSLGY 468



Score = 44.1 bits (104), Expect = 1e-06
Identities = 32/138 (23%), Positives = 54/138 (39%), Gaps = 8/138 (5%)

Query: 350 MEVIKERLERE-FGLELIATAPSVVYKVYLS-DGTVIDVQNPAEMPPKEKIER----IEE 403
ME I+ E+ +G + Y +Y S T D + A + ++ +++ + E
Sbjct: 478 MEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE 537

Query: 404 PYISASIITPAEYVGSIMQLCQDRRGIQTGFTYLDENRVELRYDMPLSEILFDFFDKLKS 463
PY+S I P EY+ T L N V L ++P I ++ L
Sbjct: 538 PYLSFKIYAPQEYLSRAYTDAPKYCANIVD-TQLKNNEVILSGEIPARCI-QEYRSDLTF 595

Query: 464 VSRGYASFDYELAGYKPS 481
+ G + EL GY +
Sbjct: 596 FTNGRSVCLTELKGYHVT 613


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0045HTHFIS882e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 88.0 bits (218), Expect = 2e-23
Identities = 32/128 (25%), Positives = 57/128 (44%), Gaps = 5/128 (3%)

Query: 6 QNINILTVDDMAAMRKILKTLLAQLGYKNVDEAEDGKQALEILKKNPNKYGLVITDWNMP 65
IL DD AA+R +L L++ GY V + + LV+TD MP
Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYD-VRITSNAATLWRWIAAGD--GDLVVTDVVMP 58

Query: 66 NMTGIELVQEIRKDPELKNIPILMVTAEAKKENVLMAIKAGVNNYIVKPFTAETLKEKIE 125
+ +L+ I+K ++P+L+++A+ + A + G +Y+ KPF L I
Sbjct: 59 DENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116

Query: 126 KIFSSLNK 133
+ + +
Sbjct: 117 RALAEPKR 124


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0046HTHFIS663e-14 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 66.0 bits (161), Expect = 3e-14
Identities = 25/128 (19%), Positives = 53/128 (41%), Gaps = 16/128 (12%)

Query: 190 KILILDDSPVARKIIRKILENDGHTVFEAQNGIEALQMLHKWLEEAKTTGRDITDYVQLI 249
IL+ DD R ++ + L G+ V N +W+ L+
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLW----RWIAAGD---------GDLV 51

Query: 250 ISDIEMPGMDGLTFTRKVKEDTEFSKIPVIINTSLSDRANVDKSRFVGADAHLVK-FDAP 308
++D+ MP + ++K+ +PV++ ++ + K+ GA +L K FD
Sbjct: 52 VTDVVMPDENAFDLLPRIKK--ARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLT 109

Query: 309 DLVKLVHQ 316
+L+ ++ +
Sbjct: 110 ELIGIIGR 117


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0047BINARYTOXINA300.024 Clostridial binary toxin A signature.
		>BINARYTOXINA#Clostridial binary toxin A signature.

Length = 454

Score = 30.4 bits (68), Expect = 0.024
Identities = 21/64 (32%), Positives = 36/64 (56%), Gaps = 11/64 (17%)

Query: 5 WEKKEIKRLEEELNSLKQKYQNLQKAYDACEKEKESLKEKLSEFSQ-KNLELSKEIEKLK 63
WEKKE +R+E+ L++L++ +A E K+ E++S +SQ + +IE
Sbjct: 60 WEKKEAERVEKNLDTLEK---------EALELYKKD-SEQISNYSQTRQYFYDYQIESNP 109

Query: 64 KEKE 67
+EKE
Sbjct: 110 REKE 113


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0049PF06580395e-05 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 39.1 bits (91), Expect = 5e-05
Identities = 10/59 (16%), Positives = 20/59 (33%), Gaps = 8/59 (13%)

Query: 383 LVRNALDHGIEPPEERVAKGKPEVGTVKLFAYHEGDHIIVGIQDDGKGIDPEKVKQKAI 441
LV N + HGI P+ G + L + + + +++ G +
Sbjct: 263 LVENGIKHGIAQ--------LPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKESTGT 313


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0050SALVRPPROT280.024 Salmonella virulence-associated 28kDa protein signature.
		>SALVRPPROT#Salmonella virulence-associated 28kDa protein signature.

Length = 241

Score = 27.8 bits (61), Expect = 0.024
Identities = 34/152 (22%), Positives = 59/152 (38%), Gaps = 14/152 (9%)

Query: 1 MEKSLLQELKELLDLIESFKSEISQISAQKAGFKAINHHIDIAILESEEATKKIIDFIGS 60
M K + KE LD+ + S A GF+ NH D+ I E+ + F G
Sbjct: 44 MRKMPVSHFKEALDVPDYSGMRQSGFFAMSQGFQLNNHGYDVFIHARRESPQSQGKFAGD 103

Query: 61 SL------EAVQESLELISQIKVKEDS---TEKAKRLRELLSATTSSLINALTLL----- 106
+ V ++ + +S + EDS K + +++ SL TL
Sbjct: 104 KFHISVLRDMVPQAFQALSGLLFSEDSPVDKWKVTDMEKVVQQARVSLGAQFTLYIKPDQ 163

Query: 107 EFQDILAQRLLKVKNFLSDIEKSILKIAILAG 138
E A L K + F+ +E + + +++G
Sbjct: 164 ENSQYSASFLHKTRQFIECLESRLSENGVISG 195


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0054IGASERPTASE338e-04 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 32.7 bits (74), Expect = 8e-04
Identities = 12/46 (26%), Positives = 22/46 (47%), Gaps = 3/46 (6%)

Query: 72 QEEKVEDTHEEQKEEVEAKEEKETQIIEKVEEEKTKEEPEKKKKEK 117
+ ++T + +E E++E KVE EKT+E P+ +
Sbjct: 1088 SGSETKETQTTETKETATVEKEEKA---KVETEKTQEVPKVTSQVS 1130



Score = 32.0 bits (72), Expect = 0.001
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 7/80 (8%)

Query: 43 KKALTELEEESLVKSESRRGKGLIITLLSQEEKVEDTHEEQKEEVEAKEEKETQIIEKVE 102
+ A + V E++ + E + E +E + E KET +EK
Sbjct: 1056 QDATETTAQNREVAKEAKSN---VKANTQTNEVAQSGSET--KETQTTETKETATVEK-- 1108

Query: 103 EEKTKEEPEKKKKEKKLSLQ 122
EEK K E EK ++ K++ Q
Sbjct: 1109 EEKAKVETEKTQEVPKVTSQ 1128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0056RTXTOXIND363e-05 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 36.0 bits (83), Expect = 3e-05
Identities = 10/28 (35%), Positives = 17/28 (60%)

Query: 112 EIEAEVSGVVKKILVENGQPVEYGQPLF 139
EI+ + +VK+I+V+ G+ V G L
Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLL 125



Score = 29.4 bits (66), Expect = 0.005
Identities = 10/22 (45%), Positives = 12/22 (54%)

Query: 88 FVKEGDFVEKGQTLCIIEALKV 109
VKEG+ V KG L + AL
Sbjct: 111 IVKEGESVRKGDVLLKLTALGA 132


22Dester_0117Dester_0124N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0117-210-0.474522sun protein
Dester_0118-2100.308994Adenylate kinase
Dester_0119-2100.315962carbamoyl-phosphate synthase, large subunit
Dester_012009-1.593941rfaE bifunctional protein
Dester_012108-1.275918Methylenetetrahydrofolate--tRNA-(uracil-5-)-
Dester_0122011-1.435588Peptidoglycan-binding lysin domain
Dester_0123-113-0.316276Phosphoglycerate kinase
Dester_0124112-2.048439nicotinate-nucleotide adenylyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0117DHBDHDRGNASE300.014 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
		>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

signature.
Length = 261

Score = 30.0 bits (67), Expect = 0.014
Identities = 16/50 (32%), Positives = 25/50 (50%)

Query: 250 QGEIILDVGAAPGGKTTALSSLTIDKARIIAVDINKERMKLLKNNLKRLG 299
+G+I GAA G +L A I AVD N E+++ + ++LK
Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEA 56


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0119HTHFIS320.017 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 31.7 bits (72), Expect = 0.017
Identities = 14/81 (17%), Positives = 27/81 (33%), Gaps = 15/81 (18%)

Query: 29 SGTQACKALKEEGYQVVLVNSNPATIMTDPDIADRTYIEPLTVEVLEKIIEKERPDALLP 88
+ + + +V+ + M D + D L I+K RPD +
Sbjct: 35 NAATLWRWIAAGDGDLVVTDVV----MPDENAFD-----------LLPRIKKARPDLPVL 79

Query: 89 TVGGQTALNLAVELYEAGILE 109
+ Q A++ E G +
Sbjct: 80 VMSAQNTFMTAIKASEKGAYD 100


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0120LPSBIOSNTHSS300.003 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 29.8 bits (67), Expect = 0.003
Identities = 20/72 (27%), Positives = 31/72 (43%), Gaps = 12/72 (16%)

Query: 30 GCFDILHAGHVDYLEKAKSLGDVLIVGMNSDSSIKRIKGEKRPIVSQDYR----AKVLIA 85
G FD + GH+D +E+ L D + V + + + K+P+ S R AK +
Sbjct: 7 GSFDPITFGHLDIIERGCRLFDQVYVAVLRNPN-------KQPMFSVQERLEQIAKAIAH 59

Query: 86 LKAVDYVFIFED 97
L V FE
Sbjct: 60 LPNA-QVDSFEG 70


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0124LPSBIOSNTHSS394e-06 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 39.0 bits (91), Expect = 4e-06
Identities = 22/96 (22%), Positives = 39/96 (40%), Gaps = 12/96 (12%)

Query: 1 MKALFGGSFNPVHIGHL-IVARDILETFGFEEIIFVPAYLQPLKDKLFLPPELRLELLRI 59
M A++ GSF+P+ GHL I+ R F+++ P K + + RLE +
Sbjct: 1 MNAIYPGSFDPITFGHLDIIERGCRL---FDQVYVAVL-RNPNK-QPMFSVQERLEQIAK 55

Query: 60 SIEEEKGFSIWDYE------IRKKGISYTVDTLREF 89
+I + +E R++ + LR
Sbjct: 56 AIAHLPNAQVDSFEGLTVNYARQRQAGAILRGLRVL 91


23Dester_0158Dester_0164N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_01582202.943324preprotein translocase, SecE subunit
Dester_01592202.593371*50S ribosomal protein L33
Dester_01600191.970690translation elongation factor Tu
Dester_01620130.904913*hypothetical protein
Dester_0163-2120.693059sigma 54 modulation protein/ribosomal protein
Dester_0164-2122.009930protein of unknown function DUF814
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0158SECETRNLCASE362e-06 Bacterial translocase SecE signature.
		>SECETRNLCASE#Bacterial translocase SecE signature.

Length = 127

Score = 35.6 bits (82), Expect = 2e-06
Identities = 15/55 (27%), Positives = 29/55 (52%)

Query: 4 ITFLKEVREELSRVTWPSKEEVIEATAGIVIFCIVVAVYFWALDFVFSELLKLII 58
+ F +E R E+ +V WP+++E + T + V+++ W LD + L+ I
Sbjct: 69 VAFAREARTEVRKVIWPTRQETLHTTLIVAAVTAVMSLILWGLDGILVRLVSFIT 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0160TCRTETOQM893e-21 Tetracycline resistance protein TetO/TetQ/TetM family ...
		>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family

signature.
Length = 639

Score = 89.1 bits (221), Expect = 3e-21
Identities = 70/320 (21%), Positives = 115/320 (35%), Gaps = 68/320 (21%)

Query: 14 NVGTIGHVDHGKTTLTAAITHCLALQGKAQEV--AYDQIDKAPEERERGITIATAHVEYE 71
N+G + HVD GKTTLT ++ + + V + D ER+RGITI T ++
Sbjct: 5 NIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQ 64

Query: 72 SDKYHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHVLLARQVNVPYI 131
+ +D PGH D++ + + +DGAIL++SA DG QTR R++ +P I
Sbjct: 65 WENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTI 124

Query: 132 VVFLNKVD-----------------------------------------------MVDDE 144
F+NK+D + ++
Sbjct: 125 -FFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGND 183

Query: 145 ELLE-------LVELEVRELLNEYDFPGDEVPVIKGSALKALECTSPDCPDCQPIYELVN 197
+LLE L LE+ + + PV GSA + I L+
Sbjct: 184 DLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIG-----------IDNLIE 232

Query: 198 ALDEYVPEPVREVDKPFLMPIEDVFSISGRGTVVTGRVERGKLTVGEEVEIVGLREEPIK 257
+ + + R + R+ G L + + V I + I
Sbjct: 233 VITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKIT 292

Query: 258 TVATGIEMFRKVLDEALPGD 277
+ T I +D+A G+
Sbjct: 293 EMYTSINGELCKIDKAYSGE 312


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0162LCRVANTIGEN290.037 Low calcium response V antigen signature.
		>LCRVANTIGEN#Low calcium response V antigen signature.

Length = 326

Score = 28.9 bits (64), Expect = 0.037
Identities = 23/83 (27%), Positives = 34/83 (40%), Gaps = 15/83 (18%)

Query: 34 NTKVTIGGELRERIEWYANDVGTGEGRDIYIPMRAKLKLKAELSDGVSAVFVPEAAFNAG 93
N ++I + R+ E +AN V T + EL + A F+PE A G
Sbjct: 43 NIDISIKYDPRKDSEVFANRVITDD---------------IELLKKILAYFLPEDAILKG 87

Query: 94 SHFHGVGTNGLDLNKEVAQDSIN 116
H+ NG+ KE + S N
Sbjct: 88 GHYDNQLQNGIKRVKEFLESSPN 110


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0164FbpA_PF05833393e-05 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 38.7 bits (90), Expect = 3e-05
Identities = 22/79 (27%), Positives = 36/79 (45%), Gaps = 2/79 (2%)

Query: 225 RHFRIGDCKLI-IGRNREENRFLRKHK-KEEDCVLWTPSIPGPTALLRCKKTPESSFLKI 282
HF D I +G+N +N +L + D T +IPG +++ S L
Sbjct: 460 MHFISKDGIDIYVGKNNIQNDYLTLKFANKHDIWFHTKNIPGSHVIVKNIMDIPESTLLE 519

Query: 283 AAEIVARYSDAKDRPSVEV 301
AA + A YS +++ +V V
Sbjct: 520 AANLAAYYSKSQNSSNVPV 538


24Dester_0379Dester_0387N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0379116-5.539022type II and III secretion system protein
Dester_0380-112-4.279400hypothetical protein
Dester_0381-111-3.057830hypothetical protein
Dester_0382012-3.050278hypothetical protein
Dester_0383011-2.463854type IV pilus assembly protein PilM
Dester_038409-1.822904Type II secretion system F domain
Dester_0385010-1.324759general secretory pathway protein E
Dester_0386-110-1.350801general secretion pathway protein D
Dester_0387012-1.852698PDZ/DHR/GLGF domain protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0379BCTERIALGSPD2006e-58 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 200 bits (510), Expect = 6e-58
Identities = 88/355 (24%), Positives = 156/355 (43%), Gaps = 27/355 (7%)

Query: 303 RILPKTKIIKQELQEPTKTYIVNLNYANAEEIEKEIKELVKKL-------------DKRE 349
RI+ K + ++ T ++ L YA A ++ + + + + DK
Sbjct: 251 RIIAMIKQLDRQQATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAAKPVAALDKNI 310

Query: 350 RITVNKSTNSLLLTVTKKHYQEIMNLLKKLDKPMKQVIVKAKIVQISTSAAKDFGFGWLI 409
I + TN+L++T ++ ++ +LD QV+V+A I ++ + + G W
Sbjct: 311 IIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDADGLNLGIQWAN 370

Query: 410 --GGYNHMGTPPSSYITGSYGFGLGSNTGVLPFINETSYSSLYNIPVGESTLALGILNKS 467
G T G + G + ++ SS I G +L
Sbjct: 371 KNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGNWAML--- 427

Query: 468 QNLKVEIALKALQLDGNAKIVSSPEVLTLNNQEATIEQGIEIPYRE-STVASGGATTYTV 526
L AL I+++P ++TL+N EAT G E+P S SG TV
Sbjct: 428 --------LTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTV 479

Query: 527 SFKRASLILKVKPHITNNNEIILDLEVRKDSPNYEHVALTGSNEPAIDTRNVKSRIKVAN 586
K + LKVKP I + ++L++E S + + +TR V + + V +
Sbjct: 480 ERKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGS 539

Query: 587 GNTIVIGGIYEKEKSKSKTGVPVISNIPLLGWLFKQESVKFTEKNLLIFITPKVV 641
G T+V+GG+ +K S + VP++ +IP++G LF+ S K +++NL++FI P V+
Sbjct: 540 GETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVI 594



Score = 53.4 bits (128), Expect = 2e-09
Identities = 26/166 (15%), Positives = 63/166 (37%), Gaps = 15/166 (9%)

Query: 237 SLKFNNADVRSVVKAIANIAGINIVFDPEVKGTVSI---DFEKPVFWKDALEAVLTPLGL 293
S F D++ + ++ ++ DP V+GT+++ D + +VL G
Sbjct: 31 SASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVLDVYGF 90

Query: 294 TYKETENYMRILPKTKIIKQELQEPT-----------KTYIVNLNYANAEEIEKEIKELV 342
N + + ++K K T +V L A ++ +++L
Sbjct: 91 AVINMNNGVLKVVRSKDAKTAAVPVASDAAPGIGDEVVTRVVPLTNVAARDLAPLLRQLN 150

Query: 343 KKLDKRERITVNKSTNSLLLTVTKKHYQEIMNLLKKLDKPMKQVIV 388
+ + +N LL+T + ++ +++++D + +V
Sbjct: 151 DN-AGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSVV 195



Score = 31.8 bits (72), Expect = 0.008
Identities = 20/87 (22%), Positives = 43/87 (49%), Gaps = 8/87 (9%)

Query: 323 IVNLNYANAEEIEKEIKELVKKLDKRE-------RITVNKSTNSLLLTVTKKHYQEIMNL 375
V L++A+A ++ K + EL K K + ++ TN++L++ Q I+ +
Sbjct: 196 TVPLSWASAADVVKLVTELNKDTSKSALPGSMVANVVADERTNAVLVSGEPNSRQRIIAM 255

Query: 376 LKKLDKPMKQVIVKAKIVQISTSAAKD 402
+K+LD+ + K++ + + A D
Sbjct: 256 IKQLDRQ-QATQGNTKVIYLKYAKASD 281


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0384BCTERIALGSPF2773e-92 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 277 bits (710), Expect = 3e-92
Identities = 115/406 (28%), Positives = 220/406 (54%), Gaps = 8/406 (1%)

Query: 1 MGVFTYKGYDKEGKERKGVIEASSRSGAISILKSQGIFPYEIKEEAIKKRNFSFSIF--- 57
M + Y+ D +GK+ +G EA S A +L+ +G+ P + E ++ +
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 58 -KKALSVQELAVFFRTLATLLDAGIPLIEAIESLSENFKEDRKKIFMTKIVNNLREGKSL 116
K LS +LA+ R LATL+ A +PL EA++++++ ++ M + + + EG SL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 117 SESLKIA-GIKDPVIVSFVSSGEKGGTLVQSLEIIASILEKREELKSTIINALIYPVVLL 175
++++K G + + + V++GE G L L +A E+R++++S I A+IYP VL
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 176 VVAIGVVVFMMLSVIPKIVSIYTSMKISLPLSTKITLFISNGFINYYHFILIFFVFLTLF 235
VVAI VV ++ V+PK+V + MK +LPLST++ + +S+ + ++L+ + +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMKQALPLSTRVLMGMSDAVRTFGPWMLLALLAGFMA 240

Query: 236 FIFLEKRKKRE--FDKFKLRLPVFGKLFLYIELNRFFETLSSLLKAGIPIVDAMISATHT 293
F + +++KR F + L LP+ G++ + R+ TLS L + +P++ AM +
Sbjct: 241 FRVMLRQEKRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISGDV 300

Query: 294 VKNEYLKERLLLINNELKKGKSLATLFSKEIKELPTVALQLIKAGEQSGRLAELLSKVSK 353
+ N+Y + RL L + +++G SL ++ P + +I +GE+SG L +L + +
Sbjct: 301 MSNDYARHRLSLATDAVREGVSL-HKALEQTALFPPMMRHMIASGERSGELDSMLERAAD 359

Query: 354 FLRNEVEIYTKNLTSMLEPAIMIIIGLIVGFIVFSLLLPIVEISTI 399
E + EP +++ + +V FIV ++L PI++++T+
Sbjct: 360 NQDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQLNTL 405


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0385PF07299290.028 Fibronectin-binding protein (FBP)
		>PF07299#Fibronectin-binding protein (FBP)

Length = 219

Score = 29.4 bits (66), Expect = 0.028
Identities = 28/148 (18%), Positives = 55/148 (37%), Gaps = 25/148 (16%)

Query: 39 LKSLFEELGFPFLEKLPQPQREVLEKVSPSFLRKERLIPLEEDENEVEVATDNPFNLEGI 98
LKSL E E L Q+E+++ V + +E N + PF
Sbjct: 42 LKSLAIEKIIHVFENLTDEQKELIDTV-LTVQNREDAESFLLKINPYVI----PFQEVTA 96

Query: 99 KKIEWIFAKPVKIVVIPFDEVN----------------KFLMAKEETEEKEEVIDQYGED 142
+ ++ +F K K+ + +E++ KF++AK +K + + G
Sbjct: 97 QTLKKLFPKAKKLKLPDMEELDMKELSYLSWIDKGSSRKFIIAK---NDKNKFVGLQGTF 153

Query: 143 ILTLQEEAPTI-QLINDILMTAVRIKAS 169
++ ++ ++ M V IK
Sbjct: 154 QSLNKKSICSLCHGHEEVGMFLVEIKGD 181


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0386BCTERIALGSPD353e-115 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 353 bits (907), Expect = e-115
Identities = 167/617 (27%), Positives = 299/617 (48%), Gaps = 32/617 (5%)

Query: 8 FTGISIAFSLPAYTKEPNKTVQINFDSVDIQDFTKVVSQAVRKNFIIPPSLKGKITIISP 67
F+ + F+ + + +F DIQ+F VS+ + K II PS++G IT+ S
Sbjct: 10 FSLTLLIFAALLFRPAAAEEFSASFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSY 69

Query: 68 KPIPKKELFNLYVAALDELGYQVVEYKDYV-KIVRNREATKESSIVKTGQI--DGGDRIL 124
+ +++ + +++ LD G+ V+ + V K+VR+++A K +++ GD ++
Sbjct: 70 DMLNEEQYYQFFLSVLDVYGFAVINMNNGVLKVVRSKDA-KTAAVPVASDAAPGIGDEVV 128

Query: 125 TYIIIPKHLEANSVRSLVRNLL--SPVGRVSIVRNSNAIVVTDKEKNVHRIETVVRRLDR 182
T ++ ++ A + L+R L + VG V SN +++T + + R+ T+V R+D
Sbjct: 129 TRVVPLTNVAARDLAPLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDN 188

Query: 183 APMKMEIASFEIKNGKVEDVEKVLKVLLDKTFAFDIAKTVPLPGRDYYHFASDKRTNTLF 242
+ + + DV K++ L T LPG + +D+RTN +
Sbjct: 189 -AGDRSVVTVPLSWASAADVVKLVTELNKDT------SKSALPGSMVANVVADERTNAVL 241

Query: 243 VVGTQKVIREVQSLLPKLDKPLNVEDGNIHIIRLNYAFAEDMAKVLSSLF------KGTS 296
V G + + +++ +LD+ GN +I L YA A D+ +VL+ + K +
Sbjct: 242 VSGEPNSRQRIIAMIKQLDRQ-QATQGNTKVIYLKYAKASDLVEVLTGISSTMQSEKQAA 300

Query: 297 RKSMGLTGEVKIVADKSSNSLIVLSSPSDFKVVKEVIDSVDIKRPQVFVEVQIVEMSMDK 356
+ L + I A +N+LIV ++P ++ VI +DI+RPQV VE I E+
Sbjct: 301 KPVAALDKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLDIRRPQVLVEAIIAEVQDAD 360

Query: 357 LLQLGVEWKFLSRG------NLVPFGGSLYG--NLPLQAGYPSASPGLLLGI--AKWRGD 406
L LG++W + G + +P ++ G S+ L
Sbjct: 361 GLNLGIQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFY 420

Query: 407 TPDIGLLLNAYAKEGGVNVIATPQILTLDNEEAEINISKVIPYSTGVKYDANNNPVISYD 466
+ +LL A + +++ATP I+TLDN EA N+ + +P TG + + +N + +
Sbjct: 421 QGNWAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEVPVLTGSQTTSGDNIFNTVE 480

Query: 467 YKDVGITLKITPHITASGEVRLKIYEKVEDVVGYANADQTA--PITSKREAKTTVDVQDG 524
K VGI LK+ P I V L+I ++V V A++ + + R V V G
Sbjct: 481 RKTVGIKLKVKPQINEGDSVLLEIEQEVSSVADAASSTSSDLGATFNTRTVNNAVLVGSG 540

Query: 525 QTLVIGGLIKSKKLTTIEKVPVLGNIPVLGNLFKKTGHQIEKTNLLVFITPRVVRSREEE 584
+T+V+GGL+ T +KVP+LG+IPV+G LF+ T ++ K NL++FI P V+R R+E
Sbjct: 541 ETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRNLMLFIRPTVIRDRDEY 600

Query: 585 NELTNDKVNLYKENIQK 601
+ ++ + + + K
Sbjct: 601 RQASSGQYTAFNDAQSK 617


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0387BCTERIALGSPC608e-13 Bacterial general secretion pathway protein C signa...
		>BCTERIALGSPC#Bacterial general secretion pathway protein C

signature.
Length = 272

Score = 60.4 bits (146), Expect = 8e-13
Identities = 41/183 (22%), Positives = 87/183 (47%), Gaps = 15/183 (8%)

Query: 89 TLKGTIICSQCSHSIVILKDKKTGKTLAVSEGKEIKGF--KVLKIYSDYVVLKKDGKEYI 146
+L G + S SI I+ K + + +E+ G+ K++ I D VVL+ G+ +
Sbjct: 96 SLTGVMAGDDDSRSIAIIS--KDNEQFSRGVNEEVPGYNAKIVSIRPDRVVLQYQGRYEV 153

Query: 147 LKLFEKENKNSLSRLNTGFENFFQVKRKDIMNEIASGNFLRYINIVPNINPE---GLKVN 203
L L+ +E+ S G + + + + AS Y++ P +N G ++N
Sbjct: 154 LGLYSQEDSGSDGV--PGAQV------NEQLQQRASTTMSDYVSFSPIMNDNKLQGYRLN 205

Query: 204 YVNRKSFIYKLGIKPGDVITSINDIHIKTPEDSFSAFEQLKNSDSITITVVRNGREVKLH 263
+ Y++G++ D+ ++N + ++ E + A E++ + + T+TV R+G+ ++
Sbjct: 206 PGPKSDSFYRVGLQDNDMAVALNGLDLRDAEQAKKAMERMADVHNFTLTVERDGQRQDIY 265

Query: 264 YEL 266
E
Sbjct: 266 MEF 268


25Dester_0516Dester_0522N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0516-111-0.808198transcriptional regulator, TrmB
Dester_0517-390.924592Integrase catalytic region
Dester_0518-1100.616692*8-amino-7-oxononanoate synthase
Dester_0519-390.824924Uncharacterized protein family UPF0126
Dester_0520-291.356314two component transcriptional regulator, Fis
Dester_0521-191.897964DNA polymerase III, subunits gamma and tau
Dester_0522192.995070Glutamate synthase (NADPH)
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0516PYOCINKILLER310.005 Pyocin S killer protein signature.
		>PYOCINKILLER#Pyocin S killer protein signature.

Length = 617

Score = 30.9 bits (69), Expect = 0.005
Identities = 28/137 (20%), Positives = 56/137 (40%), Gaps = 21/137 (15%)

Query: 71 PKKYVPLPVRRALTNRLRQMRLEFEIREDNLRKLI----DELEKRIPETKSLLDKGSQIF 126
P++YVPL V+ + R++ L+F E L + D+ + + K+L + +
Sbjct: 63 PRRYVPLQVKE----KRREIELQFRDAEKKLEASVQAELDKADAALGPAKNL----APLD 114

Query: 127 VMEGKESIVNQAIS------MISSAESTIKIAGNKPLFILECKGNLSKYMKRNVELAAIG 180
V+ +IV A+ +++ + T A N F+ + + R +
Sbjct: 115 VINRSLTIVGNALQQKNQKLLLNQKKITSLGAKN---FLTRTAEEIGEQAVREGNINGPE 171

Query: 181 EFDQFCKDEIEKLGGKY 197
+ +F E+E L Y
Sbjct: 172 AYMRFLDREMEGLTAAY 188


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0517HTHFIS290.031 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.031
Identities = 7/30 (23%), Positives = 12/30 (40%), Gaps = 3/30 (10%)

Query: 57 GNARKTCRYFGISPTTFYKWKKRYDKYGIE 86
GN K G++ T K+ + G+
Sbjct: 450 GNQIKAADLLGLNRNTLR---KKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0520HTHFIS1092e-30 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 109 bits (273), Expect = 2e-30
Identities = 30/123 (24%), Positives = 60/123 (48%), Gaps = 2/123 (1%)

Query: 2 KVLIVDDERTIRETVKEILEDEGFEIFIEEAGSKVIGAIEKLKPDILILDLFLPGISGME 61
+L+ DD+ IR + + L G+++ I + + I D+++ D+ +P + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 ILKELHERGITQSLAVVIISGHGTVETSVKAMKLGAFDFLEKPIKYDKLIEVIEDAKKYL 121
+L + + L V+++S T T++KA + GA+D+L KP +LI +I A
Sbjct: 65 LLPRIKKAR--PDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 122 SSE 124

Sbjct: 123 KRR 125



Score = 49.8 bits (119), Expect = 1e-09
Identities = 11/49 (22%), Positives = 22/49 (44%)

Query: 138 PLKKAKEEFEKTYIKQVLKRFNGDLKKAATFMEIDISNLYRKLNKYGLN 186
+ E E I L G+ KAA + ++ + L +K+ + G++
Sbjct: 428 LYDRVLAEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0521BINARYTOXINB290.049 Binary toxin B family signature.
		>BINARYTOXINB#Binary toxin B family signature.

Length = 764

Score = 28.9 bits (64), Expect = 0.049
Identities = 22/128 (17%), Positives = 40/128 (31%), Gaps = 21/128 (16%)

Query: 324 PYTALFFYLFKLSYFKDLKRISELLSGKLELSVLEKKTENTEEEINSL--EDIYIKEVND 381
L Y F F+ ++ +G L + E+ ++ E+ Y +
Sbjct: 44 SSQGLLGYYFSDLNFQAPMVVTSSTTGDLSIP---------SSELENIPSENQYFQSA-- 92

Query: 382 KKEFVEIIPKNRTAYELLKDRIGELEKRFGKRVKILEISNGN------GKK-EVKISQES 434
I K Y + + I + SN N G+ ++KI +
Sbjct: 93 -IWSGFIKVKKSDEYTFATSADNHVTMWVDDQEVINKASNSNKIRLEKGRLYQIKIQYQR 151

Query: 435 EKKIDKLL 442
E +K L
Sbjct: 152 ENPTEKGL 159


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0522PF07675300.035 Cleaved Adhesin
		>PF07675#Cleaved Adhesin

Length = 1358

Score = 29.7 bits (66), Expect = 0.035
Identities = 22/85 (25%), Positives = 33/85 (38%), Gaps = 9/85 (10%)

Query: 6 PQVAFYPFMVLRDDYKCVRCKSCVDQCSFDATYYDEDLEMIVNRNENCVNCKRCEAFCPT 65
P Y + V RD K + T ++ED + +E CV K P
Sbjct: 988 PTPTDYTYTVYRDGTKI--------KEGLTETTFEED-GVATGNHEYCVEVKYTAGVSPK 1038

Query: 66 DAIKVVKNPSTFHPDANWTEEAIRD 90
+ + V NP+ F+P N T E +
Sbjct: 1039 ECVNVTINPTQFNPVQNLTAEQAPN 1063


26Dester_0539Dester_0552N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0539-28-0.630047hypothetical protein
Dester_0540-280.020358N-acyl-phosphatidylethanolamine-hydrolyzing
Dester_0541-28-0.353713hypothetical protein
Dester_0542-28-0.429378Protein translocase subunit secA
Dester_0543-110-0.537743NLP/P60 protein
Dester_0544-113-0.091466protease Do
Dester_0545-2100.533453RNA polymerase, sigma 70 subunit, RpoD
Dester_0546090.743230OmpA/MotB domain protein
Dester_0547-1111.066375OmpA/MotB domain protein
Dester_0548-2111.453703MotA/TolQ/ExbB proton channel
Dester_0549-1111.274232D-lactate dehydrogenase (cytochrome)
Dester_0550-1121.154309Integrase catalytic region
Dester_05510120.878078Porphobilinogen synthase
Dester_05520130.703125Uroporphyrinogen III synthase HEM4
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0539GPOSANCHOR300.007 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 30.4 bits (68), Expect = 0.007
Identities = 17/106 (16%), Positives = 34/106 (32%), Gaps = 4/106 (3%)

Query: 21 QEKTKKVVPQRNRENLINQIKNLNEKLKGKDKKIKELFREITELRNQIRELKKEKEAFES 80
+ L + L + +K ++ T +I+ L+ EK A E+
Sbjct: 131 GAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEA 190

Query: 81 Q----TKEIERLDEYKRKIESLTQELAQLKGELAEKNKKIESLKTA 122
+ K +E + + + L K LA + +E
Sbjct: 191 RQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEG 236


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0542SECA11430.0 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 1143 bits (2959), Expect = 0.0
Identities = 436/859 (50%), Positives = 585/859 (68%), Gaps = 44/859 (5%)

Query: 1 MLNAILTKIFGSKNEREIKKLKPIVEKINALEPEFEKKSKEDLRALTTKWKEEISKIEDD 60
ML +LTK+FGS+N+R +++++ +V INA+EPE EK S E+L+ T +++ + K E
Sbjct: 1 MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEV- 59

Query: 61 KEKFKYMDKILPEAFAAVREAAKRTLGMRHYDVQLIGGMVLHQGKIAEMRTGEGKTLVAT 120
++ ++PEAFA VREA+KR GMRH+DVQL+GGMVL++ IAEMRTGEGKTL AT
Sbjct: 60 ------LENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTAT 113

Query: 121 LPVYLNALAGKGVHVVTVNDYLAKRDAEWMGPVYNYLGLSVGYLQNNMEKEQRKEMYSRD 180
LP YLNAL GKGVHVVTVNDYLA+RDAE P++ +LGL+VG M ++E Y+ D
Sbjct: 114 LPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAAD 173

Query: 181 ITYGTNSEFGFDYLRDNMAFSKDEKVQRELFFAIVDEADSILIDEARTPLIISGPSEENV 240
ITYGTN+E+GFDYLRDNMAFS +E+VQR+L +A+VDE DSILIDEARTPLIISGP+E++
Sbjct: 174 ITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSS 233

Query: 241 DVYYIADAIVRQLKK-----------DKHFEVDEKTKTAVLTDEGIREVEKIVSSMTGIK 289
++Y + I+ L + + HF VDEK++ LT+ G+ +E+++ GI
Sbjct: 234 EMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVK-EGIM 292

Query: 290 DF--NLYDPKFSDLLHAIIQSLRAHHLFKKDVDYVVKDGKVVIVDEFTGRIMPGRRWSDG 347
D +LY P L+H + +LRAH LF +DVDY+VKDG+V+IVDE TGR M GRRWSDG
Sbjct: 293 DEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDG 352

Query: 348 LHQAVEAKEGVKIEAENQTLATITLQNYFRLYKKLAGMTGTAETEAAELKEIYGLDVVVI 407
LHQAVEAKEGV+I+ ENQTLA+IT QNYFRLY+KLAGMTGTA+TEA E IY LD VV+
Sbjct: 353 LHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVV 412

Query: 408 PTNKPVIRKDHPDLIFKTMKAKYNAVVKEIEENYKKGRPVLVGTNSIEASEYLSRLLKKK 467
PTN+P+IRKD PDL++ T K A++++I+E KG+PVLVGT SIE SE +S L K
Sbjct: 413 PTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKA 472

Query: 468 GIPHQVLNAKHHEREAEIVAQAGRLGAVTIATNMAGRGTDILLGGNPEFLAKKELEKKGI 527
GI H VLNAK H EA IVAQAG AVTIATNMAGRGTDI+LGG+ +
Sbjct: 473 GIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAAL------ 526

Query: 528 TPEKVGEEKYQEIYKETFERYKKITEEEKEKVKALGGLYIIGTERNESRRIDNQLRGRAG 587
+ E E+ K + + V GGL+IIGTER+ESRRIDNQLRGR+G
Sbjct: 527 ----------ENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSG 576

Query: 588 RQGDPGESRFFLSLEDNLLRLFGSDRIKKMMEMMNVPDDEPITHKMVSKALENAQRRVEQ 647
RQGD G SRF+LS+ED L+R+F SDR+ MM + + E I H V+KA+ NAQR+VE
Sbjct: 577 RQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVES 636

Query: 648 QNFQIRKRLLEYDEVYNVQRKVIYDQRNKVLEGEDFKEDILYFMEEVAKEMVENYAPVNV 707
+NF IRK+LLEYD+V N QR+ IY QRN++L+ D E I E+V K ++ Y P
Sbjct: 637 RNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQS 696

Query: 708 LPDEWDLSALKKALEARFGFEFNIPSTYDELMNLSIEDAHDDREKLVKLIYDRLVKEYEK 767
L + WD+ L++ L+ F + I D+ L E L + I + ++ Y++
Sbjct: 697 LEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPEL-------HEETLRERILAQSIEVYQR 749

Query: 768 MEKLVGEGQLREIERMIMLQTLDHYWRQHLLALDHIKESIGWRGYGQRDPIVEFKKEAFQ 827
E++VG +R E+ +MLQTLD W++HL A+D++++ I RGY Q+DP E+K+E+F
Sbjct: 750 KEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFS 809

Query: 828 LFEELISNIQNGTVDGLFN 846
+F ++ +++ + L
Sbjct: 810 MFAAMLESLKYEVISTLSK 828


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0544V8PROTEASE789e-18 V8 serine protease family signature.
		>V8PROTEASE#V8 serine protease family signature.

Length = 336

Score = 77.7 bits (191), Expect = 9e-18
Identities = 35/198 (17%), Positives = 64/198 (32%), Gaps = 40/198 (20%)

Query: 77 FRDFFFHFGIPFPFDNMPDEFKTKSLGSGFIVKVKNGWAYILTNNHVIDKATKIKVKLS- 135
+ + P + SG +V G +LTN HV+D L
Sbjct: 86 HYAPVTYIQVEAP--------TGTFIASGVVV----GKDTLLTNKHVVDATHGDPHALKA 133

Query: 136 -----------DGSIYKAKVVGKDPKTDIALIKIK-------IGNKKVPTVELGDSDNIK 177
+G ++ + D+A++K IG V + ++ +
Sbjct: 134 FPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEV-VKPATMSNNAETQ 192

Query: 178 VGEFVIAVGNPYGLNWTVTHGIVSAKGRHGLGLNPIEN-FIQTDAAINPGNSGGPLCDIH 236
V + + G P + + ++ +Q D + GNSG P+ +
Sbjct: 193 VNQNITVTGYPGDKPV-------ATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEK 245

Query: 237 GKVIGINTAIVRNAQGLG 254
+VIGI+ V N
Sbjct: 246 NEVIGIHWGGVPNEFNGA 263


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0546OMPADOMAIN801e-19 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 80.4 bits (198), Expect = 1e-19
Identities = 40/135 (29%), Positives = 63/135 (46%), Gaps = 19/135 (14%)

Query: 95 VVTQEYVLLRLFNKVLFKPNSLELTPKAKEALDKVAEIIKKL-PGNYQVRIEGHTSIEEP 153
V T+ + L + VLF N L P+ + ALD++ + L P + V + G+T
Sbjct: 210 VQTKHFTLK---SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGS 266

Query: 154 TKYLPYIHDDWDLSIRRATTVAKYLVSRGVNPKKIIAVGYGNTRPLYTWKNPILQAR--- 210
Y + LS RRA +V YL+S+G+ KI A G G + P+ ++ R
Sbjct: 267 DAY------NQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAAL 320

Query: 211 ------NRRVEIYLE 219
+RRVEI ++
Sbjct: 321 IDCLAPDRRVEIEVK 335


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0547OMPADOMAIN585e-12 OMPA domain signature.
		>OMPADOMAIN#OMPA domain signature.

Length = 346

Score = 57.6 bits (139), Expect = 5e-12
Identities = 27/95 (28%), Positives = 41/95 (43%), Gaps = 16/95 (16%)

Query: 147 KLKELELP---ITIEGHTDNVPIRSKIFPSNWELSAARAVSVLRLFIQCGYDPRKLSAAG 203
+L L+ + + G+TD + + N LS RA SV+ I G K+SA G
Sbjct: 244 QLSNLDPKDGSVVVLGYTDRIGSDA----YNQGLSERRAQSVVDYLISKGIPADKISARG 299

Query: 204 CGPYRPIAPNTT---------PEGRAKNRRIEIVI 229
G P+ NT + A +RR+EI +
Sbjct: 300 MGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV 334


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0550HTHFIS290.034 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.034
Identities = 7/30 (23%), Positives = 12/30 (40%), Gaps = 3/30 (10%)

Query: 57 GNARKTCRYFGISPTTFYKWKKRYDKYGIE 86
GN K G++ T K+ + G+
Sbjct: 450 GNQIKAADLLGLNRNTLR---KKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0552INTIMIN280.030 Intimin signature.
		>INTIMIN#Intimin signature.

Length = 939

Score = 28.5 bits (63), Expect = 0.030
Identities = 20/77 (25%), Positives = 41/77 (53%), Gaps = 4/77 (5%)

Query: 1 MSVRVFFSAN--LKENQKEILKKTGFEPVSIPLIKTVPFEFSAEEVLKFSPNFTVISSKN 58
+++ +S N L ++ E++K + + +PL K +PFE+SA +L +P
Sbjct: 82 INLSTIWSLNKHLYSSESEMMKAEPGQQIILPL-KKLPFEYSALPLLGSAP-LVAAGGVA 139

Query: 59 GVKHFFSKISPEKIRSS 75
G + +K+SP+ +S+
Sbjct: 140 GHTNKLTKMSPDVTKSN 156


27Dester_0658Dester_0661N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0658-290.063099Peptidoglycan-binding lysin domain
Dester_0659-2100.041611hypothetical protein
Dester_0660-290.198093acriflavin resistance protein
Dester_0661-111-0.798624efflux transporter, RND family, MFP subunit
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0658GPOSANCHOR290.015 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 29.3 bits (65), Expect = 0.015
Identities = 13/74 (17%), Positives = 27/74 (36%), Gaps = 3/74 (4%)

Query: 42 ESLFKAYKEAKAEHKELLSKLEKCKKELELLKAKKAELEERYSTLNTEIEKLKREIAERE 101
+ A + + LE + ELE + + +I+ L+ E A E
Sbjct: 238 MNFSTADSAKIKTLEAEKAALEARQAELE---KALEGAMNFSTADSAKIKTLEAEKAALE 294

Query: 102 SLIAELQRLEKLSR 115
+ A+L+ ++
Sbjct: 295 AEKADLEHQSQVLN 308


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0659FbpA_PF05833260.037 Fibronectin-binding protein
		>FbpA_PF05833#Fibronectin-binding protein

Length = 577

Score = 26.0 bits (57), Expect = 0.037
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 2/50 (4%)

Query: 41 KYKKLKAENEKLKEELKKCSDEKEAIKSEISSLE--SNIDELETTKTELE 88
KY KLK E E+L + +E + S ++++ N DE+E K EL
Sbjct: 389 KYNKLKKSEEAANEQLLQNEEELNYLYSVLTNINNADNYDEIEEIKKELI 438


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0660ACRIFLAVINRP5840.0 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 584 bits (1507), Expect = 0.0
Identities = 227/1099 (20%), Positives = 452/1099 (41%), Gaps = 100/1099 (9%)

Query: 10 IAKRFITSKLTPLFL-LASMLIGLASIVLTPKEEEPQIVVPMVDVFIPYPGASAKEVERK 68
+A FI + L + M+ G +I+ P + P I P V V YPGA A+ V+
Sbjct: 1 MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDT 60

Query: 69 VSTEFERLIWEIKGIDYVYSIS-KPGMSLIIARFKVGENMEDSLVRLYNQLMSNLDKLPP 127
V+ E+ + I + Y+ S S G I F+ G + + + V++ N+L LP
Sbjct: 61 VTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQ 120

Query: 128 GALKPLVKPMDINDVPIVSLTLWSNKRSPYELRELTK----ELCLQLKQVENVSKTWIIG 183
+ + + ++ S+ +++ + L ++ V + G
Sbjct: 121 EVQQQGISVEKSSSSYLMVAGFVSDNPG-TTQDDISDYVASNVKDTLSRLNGVGDVQLFG 179

Query: 184 GDSKRYKILVDQDKLKNYNLSLLQIVQSIKSANVKLSAGKII------ENNTEFPVEAGE 237
+I +D D L Y L+ + ++ +K N +++AG++ + A
Sbjct: 180 AQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQT 238

Query: 238 FIKTIDDLKNLVVTVY-DGKPVYLKDVAKVVNAPIDPENYVFIGFGPNSEKKGVGKNFIK 296
K ++ + + V DG V LKDVA+V + ENY +
Sbjct: 239 RFKNPEEFGKVTLRVNSDGSVVRLKDVARVE---LGGENY---------------NVIAR 280

Query: 297 ENGNLFPAVTIAIAKKRGTNAVTVAKEILNKFEDVKKKILPSDVHVTITRNYGKTAQDKF 356
NG PA + I G NA+ AK I K +++ P + V + Q
Sbjct: 281 INGK--PAAGLGIKLATGANALDTAKAIKAKLAELQP-FFPQGMKVLYPYDTTPFVQLSI 337

Query: 357 DELMFHLGVAIFAVVIFIGLTLG-IKEAFVVSIAIPTTLALTLFVDLLTGYTLNRVTLFA 415
E++ L AI V + + L L ++ + +IA+P L T + GY++N +T+F
Sbjct: 338 HEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFG 397

Query: 416 LIFTLGLLVDDAIVVVENIHRHLKLKKLPPLQAAIYAVAEVGNPTILATFTVIAALLPMA 475
++ +GLLVDDAIVVVEN+ R + KLPP +A +++++ + + A +PMA
Sbjct: 398 MVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMA 457

Query: 476 FVSGLMGPYMRPIPVNASIAMFFSLIVAFVISPWAAYYLLRKETEKEKKKFELEKTITYR 535
F G G R + AM S++VA +++P LL+ + + +
Sbjct: 458 FFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNT 517

Query: 536 VYNKLVR---PLLDSSLKRWAFLFGVFLLMIGSVMMFYTKAVVVKLLPFDNKSEFQVVVD 592
++ V + L ++ L++ +++ + + + LP +++ F ++
Sbjct: 518 TFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLR-LPSSFLPEEDQGVFLTMIQ 576

Query: 593 MPEGTSSEETARVTKAIADYLSKIPEVTDYELYIGTSSPFDFNGLVRHYYLRKGGNVADI 652
+P G + E T +V + DY K + ++ T + F F+G + N
Sbjct: 577 LPAGATQERTQKVLDQVTDYYLKNEKANVESVF--TVNGFSFSGQAQ--------NAGMA 626

Query: 653 RVNLIDKGERKRQSHDIAREERPKIQAVARSVNPKANVKIVEVPPGPPVLSTLVA----- 707
V+L ER + A+ K V P ++ A
Sbjct: 627 FVSLKPWEERNGDE-----NSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDF 681

Query: 708 ---EIYGKDDDVRRKIAKQVAEIFRKTPG-VVDVDTLVDADHYKYEVKIDREKARKSGIT 763
+ G D + Q+ + + P +V V D ++++++D+EKA+ G++
Sbjct: 682 ELIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGVS 741

Query: 764 EEQVVQTVNIALKGASISVAHTDYDSEAVSIFIRLPRKQREGIDDILNLSVLNKEGNLIP 823
+ QT++ AL G ++ ++++ K R +D+ L V + G ++P
Sbjct: 742 LSDINQTISTALGGTYVND--FIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMVP 799

Query: 824 LREIATIVKVPAEKTIYHKNLHPVSYVIGDVAGKYEAPIYPILAINKYLSEHPLPEGYKL 883
T V + N P + G+ A + +A+ + L+ LP G
Sbjct: 800 FSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSG--DAMALMENLASK-LPAGIGY 856

Query: 884 KYTFIPPSMPKDDFKPMMKWDGEWQITYETFRDMGAAFIVALIFIYLLIVGQFQSFIIPI 943
+T G + A ++ + ++L + ++S+ IP+
Sbjct: 857 DWT------------------GMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPV 898

Query: 944 IIMIPIPLTMIGIIPGHWLMTKLLGKTTYFTATSMIGFIALAGIVVRNSIILMDFIL-MK 1002
+M+ +PL ++G++ L M+G + G+ +N+I++++F +
Sbjct: 899 SVMLVVPLGIVGVLLAATLF------NQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLM 952

Query: 1003 KREGAPLKDSIIEAGAVRFRPIVLTAAAAIVGAAVILLDP-----IFQGLAVSLLFGVFA 1057
++EG + ++ + A +R RPI++T+ A I+G + + + + ++ G+ +
Sbjct: 953 EKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVS 1012

Query: 1058 STTLTLIVIPVIYYMLEKK 1076
+T L + +PV + ++ +
Sbjct: 1013 ATLLAIFFVPVFFVVIRRC 1031



Score = 70.6 bits (173), Expect = 3e-14
Identities = 65/440 (14%), Positives = 154/440 (35%), Gaps = 70/440 (15%)

Query: 668 DIAREE-RPKIQAVARSVNPKANVKIVEVPPGPPVLSTLVAEIYGKDD----DVRRKIAK 722
DIA+ + + K+Q + + + + V + + D+ +A
Sbjct: 101 DIAQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS 160

Query: 723 QVAEIFRKTPGVVDVDTLVDADHYKYEVKIDREKARKSGITEEQVVQTV---NIALKGAS 779
V + + GV DV L A Y + +D + K +T V+ + N +
Sbjct: 161 NVKDTLSRLNGVGDV-QLFGAQ-YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ 218

Query: 780 ISVAHTDYDSEA-VSIFIRLPRKQREGIDDILNLSVLNKEGNLIPLREIATIVKVPAEKT 838
+ + SI + K E + +N +G+++ L+++A +
Sbjct: 219 LGGTPALPGQQLNASIIAQTRFKNPEEFGKVT--LRVNSDGSVVRLKDVARVELGGENYN 276

Query: 839 IYHK-NLHPVSYVIGDVAGKYEAPIYPILAINKYLSEHPLP--EGYKLKYTFIPPSMPKD 895
+ + N P + L I + L + K K + P P+
Sbjct: 277 VIARINGKPAA----------------GLGIKLATGANALDTAKAIKAKLAELQPFFPQG 320

Query: 896 DFKPMMKWDGEWQITYETFRDMGAAF----------IVALIFIYLLIVGQFQSFIIPIII 945
MK Y+T + + I+ + + L + ++ +IP I
Sbjct: 321 -----MKVL----YPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIA 371

Query: 946 MIPIPLTMIGIIPGHWLMTKLLGKTTYFTATSM-IGFIALA-GIVVRNSIILMDFILMKK 1003
+P+ ++G + G ++ ++ + + LA G++V ++I++++ +
Sbjct: 372 ---VPVVLLGTF----AILAAFG----YSINTLTMFGMVLAIGLLVDDAIVVVENVERVM 420

Query: 1004 REGAPLKDSIIEAGAVRFRPIVLTAAAAIVGAAVILL------DPIFQGLAVSLLFGVFA 1057
E E + + ++ A + + + I++ +++++ +
Sbjct: 421 MEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMAL 480

Query: 1058 STTLTLIVIPVIYYMLEKKN 1077
S + LI+ P + L K
Sbjct: 481 SVLVALILTPALCATLLKPV 500


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0661RTXTOXIND544e-10 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 54.1 bits (130), Expect = 4e-10
Identities = 41/253 (16%), Positives = 78/253 (30%), Gaps = 51/253 (20%)

Query: 80 DRVRKGEVLAVIDSSEIKPDVKKAKAALKEVEAALREIDKAVEEVKALKSAAKANYNFTQ 139
V + EVL + S IK + + E L + V A + + +
Sbjct: 177 QNVSEEEVLRLT--SLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLSRVEK 234

Query: 140 RTYQRFKRLYESEAVSKQKFDEIKTKLEEAKSKLKAIEAKEAQL---------------- 183
F L +A++K E + K EA ++L+ +++ Q+
Sbjct: 235 SRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQ 294

Query: 184 ------LEKKKGLLAKKEQVKAELSKASAFLSYTYLKSPIDGIVLKKLVDNGNLIFPQTS 237
L+K + + EL+K + +++P+ V + +
Sbjct: 295 LFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKV------QQLKVHTEGG 348

Query: 238 VFQLG---------SYPLRVHAFIDSSYAGKVKVGETLPVKLK--------NKVIMGKVT 280
V L V A + + G + VG +K ++GKV
Sbjct: 349 VVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVG--QNAIIKVEAFPYTRYGYLVGKVK 406

Query: 281 EV--DKSADPASH 291
+ D D
Sbjct: 407 NINLDAIEDQRLG 419



Score = 51.4 bits (123), Expect = 3e-09
Identities = 31/170 (18%), Positives = 72/170 (42%), Gaps = 10/170 (5%)

Query: 42 TIKIEKKDSFSGTVIPDKQI-MVSPKVVGYLKEIKVKVGDRVRKGEVLAVIDSSEIKPDV 100
++E + +G + + + P +KEI VK G+ VRKG+VL + + + D
Sbjct: 77 LGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADT 136

Query: 101 KKAKAAL---KEVEAALREIDKAVEEVKALKSAAKANYNFTQRTYQRFKRLYESEAVSKQ 157
K +++L + + + + +++E K + F + + RL ++ K+
Sbjct: 137 LKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRL---TSLIKE 193

Query: 158 KFDEIKTKLEEAKSKLKAIEAKEAQLLEKKKGLLAKKEQVKAELSKASAF 207
+F + + + + ++ K A+ L + + + E S+ F
Sbjct: 194 QFSTWQNQKYQKEL---NLDKKRAERLTVLARINRYENLSRVEKSRLDDF 240


28Dester_0749Dester_0758N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0749-1151.455169Phosphopantetheine adenylyltransferase
Dester_0751-1181.584254transposase IS4 family protein
Dester_07530232.321850Integrase catalytic region
Dester_07550242.876035CoA-substrate-specific enzyme activase
Dester_07561262.333203hypothetical protein
Dester_07570251.784474SpoVG family protein
Dester_07580222.781532protein of unknown function DUF104
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0749LPSBIOSNTHSS1871e-63 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 187 bits (476), Expect = 1e-63
Identities = 65/157 (41%), Positives = 104/157 (66%), Gaps = 3/157 (1%)

Query: 4 KAIYPGTFDPVTLGHIDIVRRGIELFQELIIGIAENPKKEPLFTLEERKKMFEESLKEVG 63
AIYPG+FDP+T GH+DI+ RG LF ++ + + NP K+P+F+++ER + +++ +
Sbjct: 2 NAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHLP 61

Query: 64 LYEKVKVKTFNSLLVEFAKKEGAVAILRGIRIISDMDHEFTMASINRKLYPEIETVFLMP 123
+V +F L V +A++ A AILRG+R++SD + E MA+ N+ L ++ETVFL
Sbjct: 62 ---NAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTT 118

Query: 124 SDEYAYLSSSAVREIAFYGGDVSQFVTKCVETKLKEK 160
S EY++LSSS V+E+A +GG+V FV V L ++
Sbjct: 119 STEYSFLSSSLVKEVARFGGNVEHFVPSHVAAALYDQ 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0753HTHFIS290.025 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 29.0 bits (65), Expect = 0.025
Identities = 7/30 (23%), Positives = 12/30 (40%), Gaps = 3/30 (10%)

Query: 57 GNARKTCRYFGISPTTFYKWKKRYDKYGIE 86
GN K G++ T K+ + G+
Sbjct: 450 GNQIKAADLLGLNRNTLR---KKIRELGVS 476


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0755SHAPEPROTEIN330.001 Bacterial cell shape determinant MreB/Mbl protein s...
		>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein

signature.
Length = 347

Score = 32.8 bits (75), Expect = 0.001
Identities = 16/40 (40%), Positives = 22/40 (55%)

Query: 203 VVFTGGGALNPLLVKLVSEKLGMEVSVPKQPQLVGAFGAA 242
+V TGGGAL L +L+ E+ G+ V V + P A G
Sbjct: 291 MVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGG 330


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0758ANTHRAXTOXNA270.031 Anthrax toxin LF subunit signature.
		>ANTHRAXTOXNA#Anthrax toxin LF subunit signature.

Length = 800

Score = 27.0 bits (59), Expect = 0.031
Identities = 25/99 (25%), Positives = 44/99 (44%), Gaps = 8/99 (8%)

Query: 48 DIEEKKKEALLEFSKKLAEKVAFVDIKVVAENEELEIFVIVLDEFESLKPVMETAL---- 103
D+ +K + +LE +L ++ F DI +V E E+ + +E S+ E
Sbjct: 84 DLLKKIPKDVLEIYSELGGEIYFTDIDLV---EHKELQDLSEEEKNSMNSRGEKVPFASR 140

Query: 104 SLYEDLGVYLPVQVISKRKLLRWKEQRNKVYDLIKKGVS 142
++E P +I+ + EQ +VY I KG+S
Sbjct: 141 FVFEKKR-ETPKLIINIKDYAINSEQSKEVYYEIGKGIS 178


29Dester_0844Dester_0854N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0844-211-2.336444riboflavin biosynthesis protein RibF
Dester_0845113-2.162111GTP-binding protein Era-like-protein
Dester_0846011-1.250858hypothetical protein
Dester_0847-210-0.586815pseudouridine synthase Rsu
Dester_0848-110-0.896588PHP domain protein
Dester_0849-18-0.080611hypothetical protein
Dester_0850090.067053hypothetical protein
Dester_08511100.586763CoA-substrate-specific enzyme activase
Dester_08522252.403279transposase IS4 family protein
Dester_08533222.813835hypothetical protein
Dester_08541212.394901Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0844LPSBIOSNTHSS310.003 Lipopolysaccharide core biosynthesis protein signat...
		>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein

signature.
Length = 166

Score = 30.9 bits (70), Expect = 0.003
Identities = 12/49 (24%), Positives = 22/49 (44%), Gaps = 1/49 (2%)

Query: 1 MKCCIVGKFESFHKGHQSLIKEAKEKCNEVFI-ISIKKWKDGIFSDKER 48
M G F+ GH +I+ ++V++ + K +FS +ER
Sbjct: 1 MNAIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQER 49


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0847UREASE280.038 Urea amidohydrolase (urease) protein signature.
		>UREASE#Urea amidohydrolase (urease) protein signature.

Length = 570

Score = 27.8 bits (62), Expect = 0.038
Identities = 11/22 (50%), Positives = 13/22 (59%)

Query: 37 PSFNVDPQKDEVLVDGELVEYE 58
P VDP+ EV DGEL+ E
Sbjct: 535 PHIEVDPETYEVRADGELLTCE 556


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0849BCTERIALGSPG317e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 31.4 bits (71), Expect = 7e-04
Identities = 11/23 (47%), Positives = 18/23 (78%)

Query: 4 KNEGFTLIELLIVITVISILFSI 26
K GFTL+E+++VI +I +L S+
Sbjct: 6 KQRGFTLLEIMVVIVIIGVLASL 28


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0850BCTERIALGSPG536e-12 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 53.4 bits (128), Expect = 6e-12
Identities = 26/78 (33%), Positives = 46/78 (58%), Gaps = 2/78 (2%)

Query: 7 LRSKELRKGFTLVELLIVIAIIAILAAIAVPQFSKYKEKAYIAAMKSDAHNVIAAEEAYF 66
+R+ + ++GFTL+E+++VI II +LA++ VP KEKA SD + A + Y
Sbjct: 1 MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYK 60

Query: 67 AENDNY--TDNGTKLGIK 82
+N +Y T+ G + ++
Sbjct: 61 LDNHHYPTTNQGLESLVE 78


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0853RTXTOXIND310.003 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 30.6 bits (69), Expect = 0.003
Identities = 19/134 (14%), Positives = 44/134 (32%), Gaps = 11/134 (8%)

Query: 46 KELKGIEEKLNKLKEEIPKLRVSIDNIRQDLSKVEEKF----PLIEK------SIKETKS 95
+ E L+K + E + I+ + + L+ K ++ E ++
Sbjct: 200 NQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQEN 259

Query: 96 YALKLNQNSEQNTEKKLLKLKEEISLQFDSVSKEISTIKKEINASKEDERQKLKELEEKI 155
++ + +L +++ EI + K EI + L ++
Sbjct: 260 KYVEAVNELRV-YKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318

Query: 156 REIEERLSNLRIPS 169
+ EER I +
Sbjct: 319 AKNEERQQASVIRA 332


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_085456KDTSANTIGN300.025 Rickettsia 56kDa type-specific antigen protein sign...
		>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein

signature.
Length = 533

Score = 29.9 bits (67), Expect = 0.025
Identities = 14/53 (26%), Positives = 29/53 (54%)

Query: 413 KGLVQISDESALKEIIKKVLGNNEKAVKQYKEGNDKQKQKAVKFLIGQVMKET 465
K LV++ + +++ ++K+ E+ K +G+ KQ+Q A + +KET
Sbjct: 379 KDLVKLQRHAGIRKAMEKLAAQQEEDAKNQGKGDCKQQQGASEKSKEGKVKET 431


30Dester_0860Dester_0866N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_0860080.117617RNA polymerase, sigma 70 subunit, RpoD
Dester_0861110-0.363267DNA primase
Dester_0862212-0.308586Lipoprotein signal peptidase
Dester_08631121.049902DNA ligase
Dester_08640140.956612outer membrane efflux protein
Dester_0865-2140.800322drug resistance transporter, EmrB/QacA
Dester_0866-2110.741721secretion protein HlyD family protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_086060KDINNERMP320.007 60kDa inner membrane protein signature.
		>60KDINNERMP#60kDa inner membrane protein signature.

Length = 548

Score = 32.2 bits (73), Expect = 0.007
Identities = 12/72 (16%), Positives = 36/72 (50%), Gaps = 7/72 (9%)

Query: 161 KKKFFAMVERIKELLPQFEKLQKQYNRNKKNLQLKREYLKTWARINFL------LRQLPL 214
K ++ +M +++ L P+ + ++++ +K+ + + L ++N L L Q+P+
Sbjct: 374 KAQYTSM-AKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPI 432

Query: 215 SYSIYETMADDI 226
++Y + +
Sbjct: 433 FLALYYMLMGSV 444


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0861RTXTOXINA300.033 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 29.9 bits (67), Expect = 0.033
Identities = 19/65 (29%), Positives = 28/65 (43%), Gaps = 3/65 (4%)

Query: 110 QIEEAGYRAASYFHSKLSSILSYLKERGISEKEAKKFLIGYAPQGYSKE---LNLNPKLA 166
++ A AA+ HS S LK+ + A LI P+ Y + LN + A
Sbjct: 12 TLQSAKQSAANKLHSAGQSTKDALKKAAEQTRNAGNRLILLIPKDYKGQGSSLNDLVRTA 71

Query: 167 KELGL 171
ELG+
Sbjct: 72 DELGI 76


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0864RTXTOXIND300.023 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 29.8 bits (67), Expect = 0.023
Identities = 8/57 (14%), Positives = 22/57 (38%)

Query: 149 LSEAKSAVEIAESYLQAARRHLKDVKAFFDEGIVPKRDLLEAKVRVRDAEEQLEKAR 205
+ + E+ + + L D + + + K +LE + + +A +L +
Sbjct: 216 RLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYK 272


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0865TCRTETB1231e-32 Tetracycline resistance protein TetB signature.
		>TCRTETB#Tetracycline resistance protein TetB signature.

Length = 458

Score = 123 bits (311), Expect = 1e-32
Identities = 90/406 (22%), Positives = 165/406 (40%), Gaps = 28/406 (6%)

Query: 17 FMTLLDTTIVDIVLPHMMSTFEAKPDDIQWVITSYMIASAIAMPVVGWLGGKIGHRNTYL 76
F ++L+ ++++ LP + + F P WV T++M+ +I V G L ++G + L
Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83

Query: 77 LGIGLFTTMSTLCGIAPN-LETMILGRVFQGIGEGLAVPMSMTLLFELFPPEKRGIAMGM 135
GI + S + + + +I+ R QG G + M ++ P E RG A G+
Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGL 143

Query: 136 FALGATFGPSLGPTIGGYLTEHLDWRWVFYVNLLPGILVIYLLMLLIKDDRKKHVADGKL 195
G +GP IGG + ++ W ++ L+P I +I + L+K +K+ G
Sbjct: 144 IGSIVAMGEGVGPAIGGMIAHYIHWSYLL---LIPMITIITVP-FLMKLLKKEVRIKGHF 199

Query: 196 DILGFILLAISLSSLITALSKGNDWGWSDEKTVLLLYTFSVSLILFILVELKVENPLVNL 255
DI G IL+++ + + + + L +S ++F+ KV +P V+
Sbjct: 200 DIKGIILMSVGIVFFMLF-TTSYSISF--------LIVSVLSFLIFVKHIRKVTDPFVDP 250

Query: 256 GLFKFTFFRYPVFSLTLFGMGVYASYFLLPLYLEKLRKFPTIEAGEILFCPAAATGIVS- 314
GL K F V + V ++P ++ + + T E G ++ P + I+
Sbjct: 251 GLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFG 310

Query: 315 LISGILMDRKILSRKASIVIGILIFIFGTHLQSKLDLEMGKTQIILFLLPWGIGMGFFFP 374
I GIL+DR+ +I + L F L F+ I
Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSF-------LTASFLLETTSWFMT-IIIVFVLGGL 362

Query: 375 ALSQVSLGNFKGEILRQASA-----LQNLLRLVGGSVGTALSTHIL 415
+ ++ + L+Q A L N + G A+ +L
Sbjct: 363 SFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL 408


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0866RTXTOXIND983e-24 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 98.0 bits (244), Expect = 3e-24
Identities = 66/384 (17%), Positives = 134/384 (34%), Gaps = 48/384 (12%)

Query: 50 ASGKIVKLFKKEYESVSKEEPLFKVDDSLYRKDVEILKAKLESLKQKKKELSEKLSRLKE 109
+ + ++ KE ESV K + L K+ D ++ L + ++ ++
Sbjct: 103 ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIEL 162

Query: 110 QLPADVKISKENLKALEKKLNQLKYQELMEKTNYETSTQKAESSLKAAEK---------G 160
++K+ E + L+ L+++ QK + L +K
Sbjct: 163 NKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLAR 222

Query: 161 LEAAKVSFNHWKNQYNRYKRLYKKRIISKEQLEEVELAYKEALYKLESAKARLQAAKGDL 220
+ + K++ + + L K+ I+K + E E Y E A L+ K
Sbjct: 223 INRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVE-------AVNELRVYKS-- 273

Query: 221 ENAKSLKNRIAIIRKQQEEVKNKIEALKEQVKISKANLKKINELSHSIRQLEEDIKSIES 280
++ I + K + + + + K NE+ +RQ ++I +
Sbjct: 274 --------QLEQIESEILSAKEEYQLVTQLFK---------NEILDKLRQTTDNIGLLTL 316

Query: 281 QIEKAKILLSHTLVKSPVEGFIAK-KWKEEGDFISPGLPVYSIY-NSKSFFVLAWIEEDK 338
++ K + +++++PV + + K EG ++ + I + V A ++
Sbjct: 317 ELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKD 376

Query: 339 IKDIKVGNKVKVELEVCKKTFKGKVYSIGTSAGSIFSLIPRDTSQ----GEYTKVTQRIP 394
I I VG +++E F Y G G + I D + G V I
Sbjct: 377 IGFINVGQNAIIKVE----AFPYTRY--GYLVGKV-KNINLDAIEDQRLGLVFNVIISIE 429

Query: 395 VKIKVEKVPPVCIKPGTNVTVYIK 418
+ + G VT IK
Sbjct: 430 ENCLSTGNKNIPLSSGMAVTAEIK 453


31Dester_0977Dester_0984N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_09771151.758015two component transcriptional regulator, winged
Dester_09780162.696844histidine kinase
Dester_09790163.404207hypothetical protein
Dester_0980-2142.408234hypothetical protein
Dester_0981-2122.326742phosphate ABC transporter, periplasmic
Dester_0982-2131.188119phosphate ABC transporter, periplasmic
Dester_0983-19-0.541350Bifunctional purine biosynthesis protein purH
Dester_0984110-1.785486Alanine racemase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0977HTHFIS912e-23 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 90.7 bits (225), Expect = 2e-23
Identities = 36/129 (27%), Positives = 67/129 (51%), Gaps = 3/129 (2%)

Query: 3 RIAVVEDDISIGNLLKRILSKEGFYVKVYSTGEALINELFEYGKEYDLIILDVMLPGMNG 62
I V +DD +I +L + LS+ G+ V++ S L + + DL++ DV++P N
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAA--GDGDLVVTDVVMPDENA 62

Query: 63 VETCRFLRERKVKVPILMLTALSEEDDKVIGLDSGADDYVTKPFGVKELLARI-RALLRR 121
+ +++ + +P+L+++A + + + GA DY+ KPF + EL+ I RAL
Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

Query: 122 KETISGTQS 130
K S +
Sbjct: 123 KRRPSKLED 131


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0978PF06580437e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 42.9 bits (101), Expect = 7e-07
Identities = 26/125 (20%), Positives = 50/125 (40%), Gaps = 24/125 (19%)

Query: 197 LKVEIDLQDIVVPCEKILVEQM-IRNLVDNAIKF-----TKKGKIEITLKRNNKEVVILI 250
L+ E + + V M ++ LV+N IK + GKI + ++N V + +
Sbjct: 240 LQFENQINP---AIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEV 296

Query: 251 KDTGKGIKEELKKHIFEKYVKSPESNGQGIGLSIVKE-IINYHNWKIDFQSEEEKG-TTF 308
++TG + K+ G GL V+E + + + + E++G
Sbjct: 297 ENTGSLALKNTKE-------------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNA 343

Query: 309 SIKIP 313
+ IP
Sbjct: 344 MVLIP 348


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0979SECA300.021 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 29.8 bits (67), Expect = 0.021
Identities = 13/79 (16%), Positives = 26/79 (32%), Gaps = 14/79 (17%)

Query: 51 EGRKPVKIEVPAAKGTP-----------LVSKDKKIKIEGKAYIHYDVDLKNGN---HNN 96
E R P+ I PA + L+ ++K+ + H+ VD K+
Sbjct: 218 EARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTER 277

Query: 97 AFKITRNYFEVRGYFNDKD 115
+ G ++ +
Sbjct: 278 GLVLIEELLVKEGIMDEGE 296


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_0984ALARACEMASE332e-115 Alanine racemase signature.
		>ALARACEMASE#Alanine racemase signature.

Length = 356

Score = 332 bits (854), Expect = e-115
Identities = 93/359 (25%), Positives = 165/359 (45%), Gaps = 22/359 (6%)

Query: 4 WAEIHLNRLFHNYREIKKISGRKKIFAVVKANAYGHGSVRISKFLEDKTDVSGFAVATFE 63
A + L L N +++ + ++++VVKANAYGHG RI + GFA+ E
Sbjct: 6 QASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGAT---DGFALLNLE 62

Query: 64 EGKELREARIRREILVMASHLEEGYKE-AVDYRLTPVIFDFEGLKLVKELD----IPFHV 118
E LRE + IL++ E +RLT + LK ++ + ++
Sbjct: 63 EAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYL 122

Query: 119 KIDTGMGRLGFLENEWNELLSELKYSK---VEGIMSHFSSADEDLDFTKEQFKKFYSFAS 175
K+++GM RLGF + + +L+ +MSHF+ A+ D + A
Sbjct: 123 KVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEH-PDGISGAMARIEQAAE 181

Query: 176 ELKQLKPEIKIHIDNSAAIPIKFDSILTHCRVGIALYGSKPYPDY----PLNLKQVMEVK 231
L+ + + NSAA ++ R GI LYG+ P + L+ VM +
Sbjct: 182 GLECRR-----SLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLS 236

Query: 232 AKVISIKELPSEFPISYSKTYKTSKQEKVAVISFGYADGLLRSLSNKGEVLINGRRCPIR 291
+++I ++ L + + Y Y ++++ +++ GYADG R VL++G R
Sbjct: 237 SEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTV 296

Query: 292 GRICMDMTIVSI-EGLKVRKGDTAIISGEKLTFEEIAEKAGTIPYEIMCDISPRVKRIY 349
G + MDM V + + G + G+++ +++A AGT+ YE+MC ++ RV +
Sbjct: 297 GTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVT 355


32Dester_1035Dester_1044N        Y        YPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1035-191.136089Trigger factor
Dester_1036-170.804177Argininosuccinate synthase
Dester_1037-18-0.554127*tRNA(Ile)-lysidine synthase
Dester_1038-2110.275831hypoxanthine phosphoribosyltransferase
Dester_1039-2120.699193ATP-dependent metalloprotease FtsH
Dester_1040-114-0.506907GTP cyclohydrolase 1
Dester_1041-116-1.112362Tetratricopeptide TPR_1 repeat-containing
Dester_1042-111-0.011154hypothetical protein
Dester_1043-19-0.214392hypothetical protein
Dester_104409-0.373811*selenium metabolism protein YedF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1035SECYTRNLCASE330.002 Preprotein translocase SecY subunit signature.
		>SECYTRNLCASE#Preprotein translocase SecY subunit signature.

Length = 437

Score = 33.2 bits (76), Expect = 0.002
Identities = 8/29 (27%), Positives = 12/29 (41%), Gaps = 1/29 (3%)

Query: 30 EICKEIKKT-AKIPGFRPGKAPINIIKKY 57
E+ +KK IPG R G+ +
Sbjct: 342 EVADNMKKYGGFIPGIRAGRPTAEYLSYV 370


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1038PHAGEIV270.043 Gene IV protein signature.
		>PHAGEIV#Gene IV protein signature.

Length = 426

Score = 26.8 bits (59), Expect = 0.043
Identities = 11/42 (26%), Positives = 18/42 (42%)

Query: 7 IPENLLRKRVRELAEEISKQFGNSSITVVSVLKGATVFTADL 48
+ +R+ SKQ G S I V TV+++D+
Sbjct: 22 QVIEMNNSSLRDFVTWYSKQTGESVIVSPDVKGTVTVYSSDV 63


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1039HTHFIS364e-04 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 36.0 bits (83), Expect = 4e-04
Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 18/82 (21%)

Query: 193 VLLAGAPGTGKTLLAKAI---AGEANVPFLSVS---------GSEF--VEMFVGVGASRV 238
+++ G GTGK L+A+A+ N PF++++ SE E GA
Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTR 222

Query: 239 RD-LFEQAKRHAPCIVFIDEID 259
FEQA+ +F+DEI
Sbjct: 223 STGRFEQAEGGT---LFLDEIG 241


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1041SYCDCHAPRONE320.001 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 32.2 bits (73), Expect = 0.001
Identities = 16/67 (23%), Positives = 28/67 (41%), Gaps = 3/67 (4%)

Query: 54 IGLSYLNIGEIPLALNYLFKAKELKPNDPKIYNAIGVAFIQRGELDRAEKYLRKAIKM-- 111
+G +G+ LA++ + +P+ +Q+GEL AE L A ++
Sbjct: 76 LGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIA 135

Query: 112 -KPNFSE 117
K F E
Sbjct: 136 DKTEFKE 142



Score = 28.7 bits (64), Expect = 0.015
Identities = 12/80 (15%), Positives = 27/80 (33%), Gaps = 4/80 (5%)

Query: 192 LAKILENEGKIEEAQEIYLRLVSLYPKVQYPYYRLALIYLKKKKIQHAKALL----KKCI 247
L + G+ + A Y + K + A L+K ++ A++ L +
Sbjct: 76 LGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIA 135

Query: 248 KLNPDSDIGIKAKIKLEELD 267
++ + LE +
Sbjct: 136 DKTEFKELSTRVSSMLEAIK 155


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1044PF01206695e-18 SirA family protein
		>PF01206#SirA family protein

Length = 76

Score = 69.0 bits (169), Expect = 5e-18
Identities = 17/69 (24%), Positives = 41/69 (59%), Gaps = 2/69 (2%)

Query: 5 IDCKGLACPIPVMKTKEALESIENG-TVTVIVDNKASRENVKRFAEKLGCK-VKVEEKKG 62
+D GL CP+P++K K+ L ++ G + V+ + S ++ + F+++ G + ++ +E+ G
Sbjct: 8 LDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKEEDG 67

Query: 63 LYYLTITKG 71
Y+ + +
Sbjct: 68 TYHFRLKRA 76


33Dester_1148Dester_1159N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1148117-5.599106two component transcriptional regulator, winged
Dester_1149013-4.680272integral membrane sensor signal transduction
Dester_1150012-4.045447phosphoesterase PA-phosphatase related protein
Dester_1151-211-3.560932outer membrane efflux protein
Dester_1152-211-1.493202efflux transporter, RND family, MFP subunit
Dester_1153-310-0.672567acriflavin resistance protein
Dester_1154-290.557571DEAD/DEAH box helicase domain protein
Dester_1155-290.473682cold-shock DNA-binding domain protein
Dester_1157-290.478421Glutamate synthase (NADPH)
Dester_1158-290.329233Methionine synthase
Dester_1159-28-0.129826Adenine phosphoribosyltransferase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1148HTHFIS956e-25 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 95.3 bits (237), Expect = 6e-25
Identities = 33/123 (26%), Positives = 62/123 (50%), Gaps = 1/123 (0%)

Query: 2 KILLVEDEKLLANTLKKGLEEEGYIVDVAYDGEEGFFLGRCYGYDVIILDVMLPKLDGME 61
IL+ +D+ + L + L GY V + + + D+++ DV++P + +
Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64

Query: 62 LLLKLREEGVKTPILMLTAKDSVEDKVKGLDSGADDYLTKPFSYDELLARI-RALLRRKS 120
LL ++++ P+L+++A+++ +K + GA DYL KPF EL+ I RAL K
Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124

Query: 121 ESK 123

Sbjct: 125 RPS 127


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1149PF06580447e-07 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 44.1 bits (104), Expect = 7e-07
Identities = 23/107 (21%), Positives = 40/107 (37%), Gaps = 27/107 (25%)

Query: 361 LLIQLFVNILENAIKYNI----YGGSVNIDVKNTEQELRVSISDTGVGIPEKEINRVFEK 416
+L+Q V EN IK+ I GG + + + + + +TG +
Sbjct: 258 MLVQTLV---ENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNT------- 307

Query: 417 FYTVKECDLKEPGTGIGLSIVKE-IAELH--RASLKIESKLNKGTTI 460
+ TG GL V+E + L+ A +K+ K K +
Sbjct: 308 ----------KESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAM 344


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1152RTXTOXIND330.002 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 32.9 bits (75), Expect = 0.002
Identities = 26/144 (18%), Positives = 52/144 (36%), Gaps = 23/144 (15%)

Query: 50 DITVEVPGQIDLS-NVCAITSKTEGYIT-TYVGKGDSVKKGKVIAEI------------Q 95
+I G++ S I + V +G+SV+KG V+ ++ Q
Sbjct: 81 EIVATANGKLTHSGRSKEIKPIENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQ 140

Query: 96 NEILKTKVLSLKNKISLVKGELLNFN-------KKLKNYQELFQLGLIS--KNDLVNLKN 146
+ +L+ ++ + +I EL +N E L L S K +N
Sbjct: 141 SSLLQARLEQTRYQILSRSIELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQN 200

Query: 147 TILNKKIELENLQHQLRILQFQEN 170
K++ L+ + + + + N
Sbjct: 201 QKYQKELNLDKKRAERLTVLARIN 224



Score = 29.8 bits (67), Expect = 0.016
Identities = 21/158 (13%), Positives = 51/158 (32%), Gaps = 45/158 (28%)

Query: 86 KKGKVIAEIQNEILKTKVLSLKNKISLVKGELLNFNKKLKNYQELFQLGLISKNDLVNLK 145
+ K E+ + + + L++ +I+ + +L ++ L I+K+ ++ +
Sbjct: 199 QNQKYQKELNLDKKRAERLTVLARINRYENLSRVEKSRLDDFSSLLHKQAIAKHAVLEQE 258

Query: 146 NT---------------------ILNKKIELEN----------------------LQHQL 162
N IL+ K E + L +L
Sbjct: 259 NKYVEAVNELRVYKSQLEQIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLLTLEL 318

Query: 163 RILQFQENYSKIISPCNGYV--LEIVPNKGYIKIGTQI 198
+ ++ S I +P + V L++ G + +
Sbjct: 319 AKNEERQQASVIRAPVSVKVQQLKVHTEGGVVTTAETL 356


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1153ACRIFLAVINRP461e-148 Acriflavin resistance protein family signature.
		>ACRIFLAVINRP#Acriflavin resistance protein family signature.

Length = 1034

Score = 461 bits (1188), Expect = e-148
Identities = 208/1040 (20%), Positives = 437/1040 (42%), Gaps = 48/1040 (4%)

Query: 8 LFILQRKFAISLTLLILSLVGYLVSKDIPRGVFPNVFFPRIEVTIENGFVPVEQMLSEVT 67
FI + FA L ++++ + G L +P +P + P + V+ + + VT
Sbjct: 4 FFIRRPIFAWVLAIILM-MAGALAILQLPVAQYPTIAPPAVSVSANYPGADAQTVQDTVT 62

Query: 68 KPAEESLKTVQDAEKIVSKT-SVGSTEINIYFDWKINPYLAYQLVQARVAELKNRLPSTA 126
+ E+++ + + + S + S GS I + F +P +A VQ ++ LP
Sbjct: 63 QVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEV 122

Query: 127 NVV-VRQATPSIYPIAIYAICSNSLPRDK--LTEILYYQLKPLFLSIKGVYDIEIKAPEW 183
+ S + + S++ + +++ + +K + GV D+++ +
Sbjct: 123 QQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQ- 181

Query: 184 EEYHVIVDLKKLANYNIDIGKVVSILREQT------KIKFLGKLDSPHKQYIISLYQKSK 237
+ +D L Y + V++ L+ Q ++ L I + K
Sbjct: 182 YAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRFK 241

Query: 238 DIYKLLKIKIPVS-NSKFISLSDIAIIVKSHTPVKSISAFSGYKNAVVFNLLRQPNANSV 296
+ + K+ + V+ + + L D+A + I+ +G K A + AN++
Sbjct: 242 NPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYNVIARING-KPAAGLGIKLATGANAL 300

Query: 297 EVVKNVDELLGKINASLKKQGIIIKKSYDSTLFIEEAIKSVRDAILLGSVIAVFIIYLFL 356
+ K + L ++ QG+ + YD+T F++ +I V + ++ ++YLFL
Sbjct: 301 DTAKAIKAKLAELQPFFP-QGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFL 359

Query: 357 RKVKLSLATLLTIPVIFFITIIGIKITKLDFNLFSLGGLAAAIGGLIDHIIIVTENIERH 416
+ ++ +L + +PV+ T + N ++ G+ AIG L+D I+V EN+ER
Sbjct: 360 QNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERV 419

Query: 417 LRKGK-DKLTAVIEGSKEIIPIMTVATLISIIVFIPLLLVSGIVGVFFKQLALVLVATYI 475
+ + K A + +I + ++ VFIP+ G G ++Q ++ +V+
Sbjct: 420 MMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA 479

Query: 476 ISQILAIFFVPIVAYILL-----PQKEEKK------VDLIERLKEKYANFLRRALKYDYL 524
+S ++A+ P + LL E K + Y N + + L
Sbjct: 480 LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGR 539

Query: 525 SVPLIIIGILTTFVLYKALPSTFLPKWDEGNLVVDFSFSPGTSLEEAYKEAMEIGKII-- 582
+ + + + VL+ LPS+FLP+ D+G + G + E K ++
Sbjct: 540 YLLIYALIVAGMVVLFLRLPSSFLPEEDQGVFLTMIQLPAGATQERTQKVLDQVTDYYLK 599

Query: 583 NSIPEVENWTLRIGTSLGHIVTQPSKGDFLVVLK-----SNRKRSIFQIKEELRAKVLS- 636
N VE+ G S + G V LK + + S + + ++
Sbjct: 600 NEKANVESVFTVNGFSFSG--QAQNAGMAFVSLKPWEERNGDENSAEAVIHRAKMELGKI 657

Query: 637 RFPNLQEFDLPQVLEDRLGDIMGAEAPISIILYGSDPEKLIKTGQYLRDILRKQPL-LEE 695
R + F++P ++E LG G I G + L + L + + P L
Sbjct: 658 RDGFVIPFNMPAIVE--LGTATGF-DFELIDQAGLGHDALTQARNQLLGMAAQHPASLVS 714

Query: 696 VNLKTNYVSPSIQISVKPDAEALYGITVNDIYNQLYSIYWGKVIGNIMQGEKI--INIRL 753
V + ++ V + G++++DI + + G + + + ++ + ++
Sbjct: 715 VRPNGLEDTAQFKLEVDQEKAQALGVSLSDINQTISTALGGTYVNDFIDRGRVKKLYVQA 774

Query: 754 LSSQKKSFFEIQKLKVYSPKLGKLIPISYVVDISFKDKVPEITHYNLSPVSVITLRF-KG 812
+ + ++ KL V S G+++P S + P + YN P I G
Sbjct: 775 DAKFRMLPEDVDKLYVRSAN-GEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPG 833

Query: 813 NNMSKAVEIIQKVIKEAKISSSISPVISGFYKEQQKSFKEMLFVIILSIVIILTALMFQF 872
+ A+ +++ + +K+ + I +G +++ S + ++ +S V++ L +
Sbjct: 834 TSSGDAMALMENL--ASKLPAGIGYDWTGMSYQERLSGNQAPALVAISFVVVFLCLAALY 891

Query: 873 GDFKISISTLLALILTLIGVFMALLLTAKPLDITAFMGMLIVLSIAINNNILIFDFYK-M 931
+ I +S +L + L ++GV +A L + D+ +G+L + ++ N ILI +F K +
Sbjct: 892 ESWSIPVSVMLVVPLGIVGVLLAATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDL 951

Query: 932 SEKNHLSETEKIVAATSTRFRPIMMTMLSNSFAMLPIALTIGSGTQILQDMAIAIIGGLL 991
EK E + A R RPI+MT L+ +LP+A++ G+G+ + I ++GG++
Sbjct: 952 MEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMV 1011

Query: 992 FAIFVNLWIIPMFFHFIKKK 1011
A + ++ +P+FF I++
Sbjct: 1012 SATLLAIFFVPVFFVVIRRC 1031


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1154SECA320.006 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 31.8 bits (72), Expect = 0.006
Identities = 21/91 (23%), Positives = 40/91 (43%), Gaps = 4/91 (4%)

Query: 229 IIFVKSEDKLKALEKLLKEHQGTSTIVFVKTK--RDAAEIEKELQKRSINARAIHGDLSQ 286
++++ +K++A+ + +KE V V T + + EL K I ++
Sbjct: 426 LVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHA 485

Query: 287 RQRENVMKAFKEGKVKTLVATDVAARGIDIK 317
+ V +A V +AT++A RG DI
Sbjct: 486 NEAAIVAQAGYPAAVT--IATNMAGRGTDIV 514


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1159SOPEPROTEIN300.006 Salmonella type III secretion SopE effector protein ...
		>SOPEPROTEIN#Salmonella type III secretion SopE effector protein

signature.
Length = 239

Score = 29.7 bits (66), Expect = 0.006
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 5/33 (15%)

Query: 34 IIFKDITPLLHK-----PWAFQKIIDYIGNRYI 61
II ++ PL ++ P FQ I++ I N+YI
Sbjct: 203 IIMTEVAPLFNECAMPTPQQFQLILENIANKYI 235


34Dester_1214Dester_1224N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1214-117-1.926734flagellar motor switch protein FliG
Dester_1215-118-2.115499flagellar motor switch protein FliM
Dester_1216019-1.871470flagellar motor switch protein FliN
Dester_1217020-2.141933fagellar hook-basal body protein
Dester_1218-116-1.335931flagellar basal-body rod protein FlgG
Dester_1219017-2.393501flagella basal body P-ring formation protein
Dester_1220016-1.922987Flagellar L-ring protein
Dester_1221114-2.259240Flagellar P-ring protein
Dester_1222216-4.555613Flagellar protein FlgJ
Dester_1223216-4.998097flagellar biosynthesis protein FlhA
Dester_1224218-6.504634flagellar biosynthetic protein FlhF
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1214FLGMOTORFLIG2482e-82 Flagellar motor switch protein FliG signature.
		>FLGMOTORFLIG#Flagellar motor switch protein FliG signature.

Length = 344

Score = 248 bits (634), Expect = 2e-82
Identities = 119/341 (34%), Positives = 202/341 (59%), Gaps = 7/341 (2%)

Query: 3 EEEKLQRTERITGAQKAAILILTLPEDIAVNVIKNLKEHELNKLAKTILTLGTIKRDMVK 62
+E+++ +TG QKAAIL++++ +I+ V K L + E+ L I L TI ++
Sbjct: 5 KEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKD 64

Query: 63 LVLKEARDEL-AEIAPLKTAPNELRRLLEKALPPEKLQKLLEETMMTESGKVIFNELQKL 121
VL E ++ + A+ K + R LLEK+L +K ++ + F +++
Sbjct: 65 NVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSR-PFEFVRRA 123

Query: 122 DPKFIAKLIEKEHPQIIAIILSQLKPTKAADVIQYLPKRLGITNVQEEVIKRLAMLEKVS 181
DP I I++EHPQ IA+ILS L P KA+ ++ LP T VQ V +R+A++++ S
Sbjct: 124 DPANILNFIQQEHPQTIALILSYLDPQKASFILSSLP-----TEVQTNVARRIALMDRTS 178

Query: 182 MKTLRIVTDALEEELASLGAGKEETLSGIDIAAEIVNNLPKEIAQDLLDEIRKENPSLAD 241
+ +R V LE++LASL + + G+D EI+N ++ + +++ + +E+P LA+
Sbjct: 179 PEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAE 238

Query: 242 SIEERMFKFEDIIKLDNRAIIEILKAVDKNDLLLALKGAPEDILNKFLSNMSKRAAQMFL 301
I+++MF FEDI+ LD+R+I +L+ +D +L ALK + K NMSKRAA M
Sbjct: 239 EIKKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLK 298

Query: 302 EDMEALGPVKKSDVEKARKKVIAIIKKLAEEGKIELGGSEE 342
EDME LGP ++ DVE++++K++++I+KL E+G+I + E
Sbjct: 299 EDMEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGE 339


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1215FLGMOTORFLIM1778e-55 Flagellar motor switch protein FliM signature.
		>FLGMOTORFLIM#Flagellar motor switch protein FliM signature.

Length = 344

Score = 177 bits (449), Expect = 8e-55
Identities = 85/340 (25%), Positives = 153/340 (45%), Gaps = 13/340 (3%)

Query: 4 EFLSQEEIDALLGGDSN-------SSSEEQKLEVAPFDFSLVEHIKKGGVPGLELLLERW 56
E LSQ+EID LL S+ + ++ +DF + K + L L+ E +
Sbjct: 3 EVLSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETF 62

Query: 57 IKIYSEEIRRLVPQINMVTKESVYITRFNNFMSKIPLPASYSIVSMKPLKDNFLLVLDSR 116
++ + + + + V SV + F+ IP P++ ++++M PLK N +L +D
Sbjct: 63 ARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVLEVDPS 122

Query: 117 LVFVVISVMFGGPAQPFKIEGREFTKLETRIIDDIVKISLSTFQHTWKDVYPVEFELKSI 176
+ F +I +FGG Q K++ R+ T +E +++ ++ L+ + +W V + L I
Sbjct: 123 ITFSIIDRLFGGTGQAAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRPRLGQI 181

Query: 177 ELNPALARIVSGNDRVIVVECTMDVDGYEAPFFFCFPQGMFLPIKELIFS----EAVFAE 232
E NP A+IV ++ V++V V E FC P PI + S +V
Sbjct: 182 ETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFSSVRRS 241

Query: 233 KDPVWEKHLTKKLLKTELKLTLELTRKNFFLRELLSWKEGDEILL-DISKDEFVKLYVED 291
+ L KL ++ + E+ +R++L + GD I L D + L + +
Sbjct: 242 STTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFVLSIGN 301

Query: 292 KPKFWAKLGKIKDKYAALVVDMINGENNGGKREKSGTEPE 331
+ KF + G + K AA +++ I + E S E E
Sbjct: 302 RKKFLCQPGVVGKKIAAQILERIESTSQEDFEELSADEEE 341


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1216FLGMOTORFLIN894e-26 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 89.2 bits (221), Expect = 4e-26
Identities = 41/117 (35%), Positives = 72/117 (61%)

Query: 4 WENALKEQESLEGEEEQVEEKEVSDYERKEEEEEKLELLKDIPLEVSIEIGSTSLPLEEI 63
W +AL EQ++ + + + ++L+ DIP+++++E+G T + ++E+
Sbjct: 19 WADALNEQKATTTKSAADAVFQQLGGGDVSGAMQDIDLIMDIPVKLTVELGRTRMTIKEL 78

Query: 64 LKLHTNSIVELDRYIHEPVDIKINGKLVAKGKLYTIRENYGIKITQIITPEERMKLL 120
L+L S+V LD EP+DI ING L+A+G++ + + YG++IT IITP ERM+ L
Sbjct: 79 LRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSERMRRL 135


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1217FLGHOOKAP1300.006 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 30.3 bits (68), Expect = 0.006
Identities = 10/32 (31%), Positives = 18/32 (56%)

Query: 8 LYVLAAGGERAVEQLDTAANNIANINTPGFKK 39
+ +G A L+TA+NNI++ N G+ +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTR 35


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1218FLGHOOKAP1482e-08 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 48.0 bits (114), Expect = 2e-08
Identities = 12/43 (27%), Positives = 22/43 (51%)

Query: 223 LEASNVNIVEEMVNLIVAQRAYEVNSKGITTADEMLRVVGTLK 265
S VN+ EE NL Q+ Y N++ + TA+ + + ++
Sbjct: 504 QSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 46.5 bits (110), Expect = 5e-08
Identities = 17/79 (21%), Positives = 35/79 (44%), Gaps = 14/79 (17%)

Query: 5 LWTSATGMEAQQTNLDVISHNIANVNTVGFKKSRANFEDLIYQDIRDPGVMSSEENRVPS 64
+ + +G+ A Q L+ S+NI++ N G+ + +M+ + + +
Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT--------------IMAQANSTLGA 49

Query: 65 GIQIGLGVKVSDVSKIFTQ 83
G +G GV VS V + +
Sbjct: 50 GGWVGNGVYVSGVQREYDA 68


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1220FLGLRINGFLGH1481e-46 Flagellar L-ring protein signature.
		>FLGLRINGFLGH#Flagellar L-ring protein signature.

Length = 232

Score = 148 bits (375), Expect = 1e-46
Identities = 58/202 (28%), Positives = 101/202 (50%), Gaps = 15/202 (7%)

Query: 38 KPLPPPPVEEAKPTSPGSLFS-------GYDNLFADTKARRVGDIVTVKIYEVLTGYGST 90
+P+P P P + GS+F GY LF D + R +GD +T+ + E ++ S+
Sbjct: 38 QPVPGP-----TPVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSS 92

Query: 91 QSQSGKKSTFDINVNNPTLFGKKIPNGTKDPLLNFSTKPSIDFSGQGTTKRDAKLIATIS 150
+ + + + + + + + + F+G+G T++
Sbjct: 93 SANASRDGKTNFGFDTV---PRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLT 149

Query: 151 ARVVKVYPNGNLYIVGEKIVKINDDTQILKISGIVKPTDIAPDNSVPSSKIANMYVEYNG 210
V +V NGNL++VGEK + IN T+ ++ SG+V P I+ N+VPS+++A+ +EY G
Sbjct: 150 VTVDQVLVNGNLHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVG 209

Query: 211 KGYIADNQKPGWLARFLIKIWP 232
GYI + Q GWL RF + + P
Sbjct: 210 NGYINEAQNMGWLQRFFLNLSP 231


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1221FLGPRINGFLGI347e-121 Flagellar P-ring protein signature.
		>FLGPRINGFLGI#Flagellar P-ring protein signature.

Length = 373

Score = 347 bits (892), Expect = e-121
Identities = 137/346 (39%), Positives = 200/346 (57%), Gaps = 7/346 (2%)

Query: 24 VKIGTEVNIVGVRPNYLTGYGIVVGLDGTGDGTTSI-FTLQSIANMLKKMGIYVDPKAVK 82
+I ++ R N L GYG+VVGL GTGD S FT QS+ ML+ +GI
Sbjct: 29 SRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQGGQSN 88

Query: 83 TKNAAAVIVTAKLPPFAKPGMTFDVEVASLGDAKSLANGILIRTPLLGPDGKIYAFAQGP 142
KN AAV+VTA LPPFA PG DV V+SLGDA SL G LI T L G DG+IYA AQG
Sbjct: 89 AKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQIYAVAQGA 148

Query: 143 VSTGGGFTESNKGGKIKKNFSTTGMIPNGGIVERELPFELSNEKNLILTLKHPDFSRANN 202
+ GF+ + + +T+ +PNG I+ERELP + + NL+L L++PDFS A
Sbjct: 149 LIV-NGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPDFSTAVR 207

Query: 203 IAFAINNY----FGKNLAKAEDSSTVIVKYLPNYNKVKFISEILNLKINTESEPTIVIYE 258
+A +N + +G +A+ DS + V+ + + ++EI NL + T++ +VI E
Sbjct: 208 VADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPAKVVINE 267

Query: 259 RTGTVIMSGDIKIEPPVYVSHGNIYVSVTKTPVISQPPPLSNGTTVQTENVTTTVKEEHG 318
RTGT+++ D++I V VS+G + V VT++P + QP P S G T +E
Sbjct: 268 RTGTIVIGADVRIS-RVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMAMQEGS 326

Query: 319 RIFSITSPSLRDLVKALNDLGVSPGDLIAIIQAMKAAGKLHAKIII 364
++ + P LR LV LN +G+ +IAI+Q +K+AG L A++++
Sbjct: 327 KVAIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQAELVL 372


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1222FLGFLGJ396e-07 Flagellar protein FlgJ signature.
		>FLGFLGJ#Flagellar protein FlgJ signature.

Length = 313

Score = 38.9 bits (90), Expect = 6e-07
Identities = 20/94 (21%), Positives = 43/94 (45%), Gaps = 9/94 (9%)

Query: 10 WDIANIKQIK---------NESEAIKEFEAYFVRIFLKEARKSIPKGLFNTSFSANFYYD 60
WD ++ ++K N ++ E FV++ LK R ++PK +S Y
Sbjct: 13 WDAQSLNELKAKAGEDPAANIRPVARQVEGMFVQMMLKSMRDALPKDGLFSSEHTRLYTS 72

Query: 61 MLDMELAEVISQKDPLHLEKFLQEALSKYQKISK 94
M D ++A+ ++ L L + + + ++ Q + +
Sbjct: 73 MYDQQIAQQMTAGKGLGLAEMMVKQMTPEQPLPE 106


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1224CHANLCOLICIN362e-04 Channel forming colicin signature.
		>CHANLCOLICIN#Channel forming colicin signature.

Length = 522

Score = 36.2 bits (83), Expect = 2e-04
Identities = 25/139 (17%), Positives = 50/139 (35%), Gaps = 8/139 (5%)

Query: 17 QAKRELGEEIDILYYEVEKERSFLPFFRKKKYKLFVVPKEKRENNEIEKLEEELNEVREL 76
+EL E + L PFF + ++ + + ++ E +N +
Sbjct: 245 AKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINAD 304

Query: 77 LTNIKSSL--EENKLANSSLPIPEHIDSTSSCEENLTTEFTGDALELIKVLIQK-----G 129
+T I+ ++ N + E ++ + NL DA++ Q G
Sbjct: 305 ITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKDAVDATVSFYQTLTEKYG 364

Query: 130 VK-KNIAEELVKEACGLDI 147
K +A+EL ++ G I
Sbjct: 365 EKYSKMAQELADKSKGKKI 383


35Dester_1231Dester_1244N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1231-115-1.805691Flagellar hook-basal body complex protein fliE
Dester_1232-115-1.777070flagellar M-ring protein FliF
Dester_1233116-2.381498hypothetical protein
Dester_1234216-1.715507ATPase, FliI/YscN family
Dester_1235216-2.433907flagellar hook capping protein
Dester_1236317-2.592544fagellar hook-basal body protein
Dester_1237317-5.167056flagellar basal body-associated protein FliL
Dester_1238118-4.836667surface presentation of antigens (SPOA) protein
Dester_1239116-4.761836hypothetical protein
Dester_1240016-5.336950flagellar biosynthetic protein FliP
Dester_1241016-5.381285flagellar biosynthetic protein FliQ
Dester_1242-214-4.567949flagellar biosynthetic protein FliR
Dester_1243-112-3.350564flagellar biosynthetic protein FlhB
Dester_1244211-3.016030hypothetical protein
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1231FLGHOOKFLIE571e-14 Flagellar hook-basal body complex protein FliE signa...
		>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE

signature.
Length = 103

Score = 57.4 bits (138), Expect = 1e-14
Identities = 21/81 (25%), Positives = 41/81 (50%), Gaps = 1/81 (1%)

Query: 16 KEKHNQKANGFKDLLENFIKDVNSDLKESRKAEENLISGNVQ-NIEEIMYKIEKADLSLR 74
+E Q F L + ++ +R E G + ++M ++KA +S++
Sbjct: 23 QESLPQPTISFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQ 82

Query: 75 LLVEIRNKALESYQEIMRMQV 95
+ +++RNK + +YQE+M MQV
Sbjct: 83 MGIQVRNKLVAAYQEVMSMQV 103


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1232FLGMRINGFLIF357e-119 Flagellar M-ring protein signature.
		>FLGMRINGFLIF#Flagellar M-ring protein signature.

Length = 559

Score = 357 bits (917), Expect = e-119
Identities = 170/558 (30%), Positives = 290/558 (51%), Gaps = 54/558 (9%)

Query: 7 QTKVQEIFKK-NANPKNVILLLSALTLVSFLAFIAIKQSTTEDYAVLYTHLSPDDAGSIL 65
Q K E + ANP+ I L+ A + + + + T DY L+++LS D G+I+
Sbjct: 9 QPKPLEWLNRLRANPR--IPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIV 66

Query: 66 SVLQEEHIPYKVEGNGSIILVPKEKVYDIRLKLAAKGLPHGKVVGFELFDEPKLGITQFQ 125
+ L + +IPY+ I VP +KV+++RL+LA +GLP G VGFEL D+ K GI+QF
Sbjct: 67 AQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFS 126

Query: 126 ENVEFLRALEGEIERTIKRINAIQDVKVNIALPKDSIFVRESEEPKASILVNLWPGRELT 185
E V + RALEGE+ RTI+ + ++ +V++A+PK S+FVRE + P AS+ V L PGR L
Sbjct: 127 EQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRALD 186

Query: 186 KEQVKAIVFLVSHAVPGLKPENVTVIDNRGRVLTDLLSGNEDETGSSKELEVKRKLEKEI 245
+ Q+ A+V LVS AV GL P NVT++D G +LT S + +L+ +E I
Sbjct: 187 EGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQ--SNTSGRDLNDAQLKFANDVESRI 244

Query: 246 ERKIQSMLSQVLGSGKVVVRASVEIETGRLEKKEELYDPDMTAVVSERKIQEKETGIK-- 303
+R+I+++LS ++G+G V + + +++ E+ EE Y P+ A + + ++ +
Sbjct: 245 QRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVG 304

Query: 304 -PKEQGVPGTTTNVP-PVLNLNQGNEILKKE----------------------KKDVTTN 339
GVPG +N P P ++ +++ T+N
Sbjct: 305 AGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSN 364

Query: 340 YDVSKTIQKTITPIFKIKRISVGVLVDGKYQKEKDKAGNEIIKFVPRSQEEIKTYEEIVK 399
Y+V +TI+ T + I+R+SV V+V+ K + K +P + +++K E++ +
Sbjct: 365 YEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADG--------KPLPLTADQMKQIEDLTR 416

Query: 400 SAIGYDPKRGDTVTVASVPFEAKQFVQKEK---SQKKFPWIYIAAGGGLLTLILVGIIAL 456
A+G+ KRGDT+ V + PF A E Q+ F +AAG LL L++ I+
Sbjct: 417 EAMGFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWR 476

Query: 457 KLLKSK--------KTEPQQPEIPETLMAEMKARAEHKEEL----EELHIESDPLYIKIV 504
K ++ + K +Q ++ + ++ R E+L + ++ + +I
Sbjct: 477 KAVRPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIR 536

Query: 505 EIAKEHPELVANVISKWI 522
E++ P +VA VI +W+
Sbjct: 537 EMSDNDPRVVALVIRQWM 554


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1236FLGHOOKAP1457e-07 Flagellar hook-associated protein signature.
		>FLGHOOKAP1#Flagellar hook-associated protein signature.

Length = 546

Score = 44.9 bits (106), Expect = 7e-07
Identities = 16/52 (30%), Positives = 31/52 (59%)

Query: 516 VISKVRSGMLEMSNVDIASEFINLITAQRAYQANARVITTDDQILQETMNIK 567
V++++ + +S V++ E+ NL Q+ Y ANA+V+ T + I +NI+
Sbjct: 495 VVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546



Score = 33.8 bits (77), Expect = 0.002
Identities = 15/59 (25%), Positives = 30/59 (50%), Gaps = 4/59 (6%)

Query: 4 SFYTAFTGLNADKTWLSVISDNIANVNTVGFKKENAVFEDLLARSLTTFKNGAPVNQEI 62
A +GLNA + L+ S+NI++ N G+ ++ + +A++ +T G V +
Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTI----MAQANSTLGAGGWVGNGV 57


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1238FLGMOTORFLIN545e-13 Flagellar motor switch protein FliN signature.
		>FLGMOTORFLIN#Flagellar motor switch protein FliN signature.

Length = 137

Score = 53.8 bits (129), Expect = 5e-13
Identities = 20/73 (27%), Positives = 46/73 (63%)

Query: 8 LEDFKDVSLSISLCIGKKFLTLNKILKLKEGDLIEFDKKLEDYLDVYLNGQKFGIGELVI 67
++ D+ + +++ +G+ +T+ ++L+L +G ++ D + LD+ +NG GE+V+
Sbjct: 54 IDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVV 113

Query: 68 VNDKYSLRLVDLV 80
V DKY +R+ D++
Sbjct: 114 VADKYGVRITDII 126


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1240FLGBIOSNFLIP2346e-80 Escherichia coli: Flagellar biosynthetic protein Fl...
		>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP

signature.
Length = 245

Score = 234 bits (598), Expect = 6e-80
Identities = 100/208 (48%), Positives = 151/208 (72%), Gaps = 1/208 (0%)

Query: 35 GNLDITLKILFLITILSLAPAILITVTSFTRIVIILSLLRHALGTPQTPPNQVIIALSLF 94
+ + ++ L IT L+ PAIL+ +TSFTRI+I+ LLR+ALGTP PPNQV++ L+LF
Sbjct: 36 QSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALF 95

Query: 95 LTLFTMAPTFQQIDELAIQPYINKKISDVEAIKRASEPIKNFMLRNTRKEDLKLFLDIRN 154
LT F M+P +I A QP+ +KIS EA+++ ++P++ FMLR TR+ DL LF + N
Sbjct: 96 LTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLAN 155

Query: 155 E-KPSSPQEISMLTLIPAFMVSEIRTALEVVFVIFLPFIVIDLLVASILMSMGMMMIPPM 213
P+ + M L+PA++ SE++TA ++ F IF+PF++IDL++AS+LM++GMMM+PP
Sbjct: 156 TGPLQGPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPA 215

Query: 214 MLSLPFKLILFVLSDGWELLIKSIILSY 241
++LPFKL+LFVL DGW+LL+ S+ S+
Sbjct: 216 TIALPFKLMLFVLVDGWQLLVGSLAQSF 243


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1241TYPE3IMQPROT601e-15 Type III secretion system inner membrane Q protein ...
		>TYPE3IMQPROT#Type III secretion system inner membrane Q protein

family signature.
Length = 86

Score = 59.8 bits (145), Expect = 1e-15
Identities = 21/77 (27%), Positives = 41/77 (53%)

Query: 3 VDQVITLGQKMLEIALLVGMPVLLTTFLVGIIISIFQAATQIHEMTLTFIPKIVAALLAL 62
+D ++ G K L + L++ + ++G+++ +FQ TQ+ E TL F K++ L L
Sbjct: 1 MDDLVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCL 60

Query: 63 FIFGSWMLIKLIDYTKE 79
F+ W L+ Y ++
Sbjct: 61 FLLSGWYGEVLLSYGRQ 77


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1242TYPE3IMRPROT996e-27 Type III secretion system inner membrane R protein ...
		>TYPE3IMRPROT#Type III secretion system inner membrane R protein

family signature.
Length = 261

Score = 99.0 bits (247), Expect = 6e-27
Identities = 55/251 (21%), Positives = 113/251 (45%), Gaps = 5/251 (1%)

Query: 9 TFSLFLLTFVRVASFFLAFPFISTTLIPLNIRILLILAFSFYLSQIIEPSQMIDITKIDL 68
+L+ +RV + P +S +P +++ + ++ I PS + +
Sbjct: 12 WLNLYFWPLLRVLALISTAPILSERSVPKRVKL----GLAMMITFAIAPSLPANDVPVFS 67

Query: 69 LSFFLLVIKEVLLGISFSILTTIYSSIFIHAAELISYSMGLTIVNIFDSTFGS-ISVLSR 127
L ++++L+GI+ + A E+I MGL+ D + VL+R
Sbjct: 68 FFALWLAVQQILIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLAR 127

Query: 128 FFVYIFYVVFFFTDAYKIFIAAFVESFKIIPIGNFHLSDSLLYFFLKESKLIFFLSFKIA 187
+ ++F + + I+ V++F +PIG L+ + K LIF +A
Sbjct: 128 IMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGGEPLNSNAFLALTKAGSLIFLNGLMLA 187

Query: 188 FPFIITLFITNLILALVNRLIPQINVFIVGLPLQIFIGLFFLSTGFSILIYSSKYLIEKL 247
P I L NL L L+NR+ PQ+++F++G PL + +G+ ++ ++ ++L ++
Sbjct: 188 LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEI 247

Query: 248 STDIINLIKIL 258
+ ++I L
Sbjct: 248 FNLLADIISEL 258


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1243TYPE3IMSPROT328e-114 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 328 bits (843), Expect = e-114
Identities = 105/341 (30%), Positives = 178/341 (52%), Gaps = 3/341 (0%)

Query: 7 KTEKATPRRRQKAKEEGQVLKSQDIPIAFTLLITSTLLYFYIPFAYKKLLQLFTFDFRTS 66
KTE+ TP++ + A+++GQV KS+++ ++ S +L + ++ +L S
Sbjct: 5 KTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAEQS 64

Query: 67 NNVNLWN-NYLVS--AKTFALLILPVFLVLFLGGIFSNIIQFGFLFSLKPLLPKLDNINP 123
+Y+V F L P+ V L I S+++Q+GFL S + + P + INP
Sbjct: 65 YLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVVQYGFLISGEAIKPDIKKINP 124

Query: 124 IKGLGRLFSLKTLFETFRNTLKLIIALAVGYFSGKYILSDFFSLSFISLNNQIILMLKYT 183
I+G R+FS+K+L E ++ LK+++ + + K L L + L+ +
Sbjct: 125 IEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITPLLGQIL 184

Query: 184 LLLFFIFGLLSLPIAAADFLFRRWEYEENLKMSKEEIKEERKQYEGHPLIKSAIRRKQRE 243
L I + + I+ AD+ F ++Y + LKMSK+EIK E K+ EG P IKS R+ +E
Sbjct: 185 RQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKRRQFHQE 244

Query: 244 IAMKRMMAEIPKADVVITNPTHYAVALRYERGKMHAPKVIAKGVDNIALKIKKIALEHNI 303
I + M + ++ VV+ NPTH A+ + Y+RG+ P V K D ++KIA E +
Sbjct: 245 IQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKIAEEEGV 304

Query: 304 PIEENPYLARVLYESCDIGSFIPEEFYQAIAKILAKVYKKK 344
PI + LAR LY + +IP E +A A++L + ++
Sbjct: 305 PILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLERQN 345


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1244IGASERPTASE270.031 IgA-specific serine endopeptidase (S6) signature.
		>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature.

Length = 1541

Score = 27.3 bits (60), Expect = 0.031
Identities = 14/75 (18%), Positives = 29/75 (38%), Gaps = 2/75 (2%)

Query: 21 QEIEKHEAQKEIERLKKLKVEVQRLLEEKKNLLKKIEEEKKQLEEEKKAFEKKIKEIESE 80
++ + E+ + E Q E K +EEK ++E EK K+ S
Sbjct: 1074 SNVKANTQTNEVAQSGSETKETQTT--ETKETATVEKEEKAKVETEKTQEVPKVTSQVSP 1131

Query: 81 RYKKLAQIFEKMDPE 95
+ ++ + + +P
Sbjct: 1132 KQEQSETVQPQAEPA 1146


36Dester_1269Dester_1275N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1269-18-0.838654cytochrome c-type biogenesis protein CcsB
Dester_127009-1.962890ribonuclease BN
Dester_1271010-0.158054Pantothenate synthetase
Dester_1272010-0.400645protein of unknown function UPF0047
Dester_1273110-0.273291protein of unknown function DUF77
Dester_1274210-0.268110ATP-dependent DNA helicase RecG
Dester_12754170.573420Tetratricopeptide TPR_1 repeat-containing
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1269PF06580300.015 Sensor histidine kinase
		>PF06580#Sensor histidine kinase

Length = 349

Score = 29.8 bits (67), Expect = 0.015
Identities = 19/116 (16%), Positives = 42/116 (36%), Gaps = 7/116 (6%)

Query: 179 WTVIFVTTWFIFFFVIYSAFRENVFFFLFVSAAVAAAFCAFLYLLNRYGISKIFPSPETM 238
W V + T F F + S ++ F + +S A+ + R G K+ +
Sbjct: 20 WGV-YTLTGFGFASLYGSPKLHSMIFNIAISLMGLVLTHAYRSFIKRQGWLKLNMGQIIL 78

Query: 239 EQIMYQSVAVGFVFLTIGIILGAVWAKYAWGGYWSWDPKETWSLITWLVYAAYLHA 294
+++ V +G V+ + ++W A+ T L +++ +
Sbjct: 79 -RVLPACVVIGMVWF---VANTSIWRLLAFINT--KPVAFTLPLALSIIFNVVVVT 128


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1270TYPE3IMSPROT290.016 Type III secretion system inner membrane S protein ...
		>TYPE3IMSPROT#Type III secretion system inner membrane S protein

family signature.
Length = 354

Score = 29.3 bits (66), Expect = 0.016
Identities = 22/153 (14%), Positives = 54/153 (35%), Gaps = 19/153 (12%)

Query: 124 LAGFFYRNLAVILAIFLLWIFMFVFYLAKYLIAALLPQIPILSILSSLLVPILLFAILIS 183
L+ +++ + + ++ I ++ Y++ +L + L + ++ A +
Sbjct: 45 LSDYYFEHFSKLMLIPAEQSYLPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIASHVV 104

Query: 184 MYYFLL---PIKIRFNFI---------FKISTFVFLLLTVFEKIFIWFIFNV-------S 224
Y FL+ IK I F I + V L ++ + + + + + +
Sbjct: 105 QYGFLISGEAIKPDIKKINPIEGAKRIFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVT 164

Query: 225 KVSILYSSFAAIIIFLLWIYYSAIVILIGVGIV 257
+ + I L I +VI +V
Sbjct: 165 LLQLPTCGIECITPLLGQILRQLMVICTVGFVV 197


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1274SECA340.002 SecA protein signature.
		>SECA#SecA protein signature.

Length = 901

Score = 34.5 bits (79), Expect = 0.002
Identities = 19/80 (23%), Positives = 31/80 (38%), Gaps = 5/80 (6%)

Query: 416 RLVQGDV-----GSGKTVVAAAAAFFAAKSGYQTAVMAPTEILANQHFKKFREFLKPFSI 470
L + + G GKT+ A A+ A +G V+ + LA + + R + +
Sbjct: 93 VLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGL 152

Query: 471 KVGLLTGSMTKKEKETMYRA 490
VG+ M K Y A
Sbjct: 153 TVGINLPGMPAPAKREAYAA 172


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1275SYCDCHAPRONE497e-09 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 48.8 bits (116), Expect = 7e-09
Identities = 20/116 (17%), Positives = 48/116 (41%), Gaps = 1/116 (0%)

Query: 289 INRLVKLDPYNLRLLSWVAASLFEMKEYKKVIPLIERITKLNPDNPNVYFMLGLAYEMSG 348
I L ++ L L +A + ++ +Y+ + + + L+ + + LG + G
Sbjct: 25 IAMLNEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMG 84

Query: 349 NYEKALEAYEKSLDLYPENPTVLEKTAFLLYKMNRLSDAKAYFERLWQLT-NKPGY 403
Y+ A+ +Y + + P A L + L++A++ +L +K +
Sbjct: 85 QYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEF 140



Score = 44.9 bits (106), Expect = 1e-07
Identities = 35/143 (24%), Positives = 53/143 (37%), Gaps = 7/143 (4%)

Query: 456 GYLKKLLQIEP-SPDVYNYLAYFYANRGINLDEAEKLAEKALKAEPENPAFLDTLGWVLY 514
G + L +I + + LA+ G ++A K+ + + + F LG
Sbjct: 23 GTIAMLNEISSDTLEQLYSLAFNQYQSG-KYEDAHKVFQALCVLDHYDSRFFLGLGACRQ 81

Query: 515 KKGDYKNACKYLEKALKLKQDDPVISEHYGECLYKTGRLKEAKEYLLKASSKIEKDPSIQ 574
G Y A + +P H ECL + G L EA+ L A I
Sbjct: 82 AMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKT--- 138

Query: 575 KEEKGILQRIRKILREIK-IKEM 596
E K + R+ +L IK KEM
Sbjct: 139 -EFKELSTRVSSMLEAIKLKKEM 160



Score = 39.1 bits (91), Expect = 1e-05
Identities = 20/114 (17%), Positives = 36/114 (31%), Gaps = 3/114 (2%)

Query: 255 LEKVLDKNPDNIYALKEIFIIYLKQNKTNEALNVINRLVKLDPYNLRLLSWVAASLFEMK 314
L ++ + +Y+L + K +A V L LD Y+ R + A M
Sbjct: 28 LNEISSDTLEQLYSLA---FNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMG 84

Query: 315 EYKKVIPLIERITKLNPDNPNVYFMLGLAYEMSGNYEKALEAYEKSLDLYPENP 368
+Y I ++ P F G +A + +L +
Sbjct: 85 QYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKT 138


37Dester_1316Dester_1324N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_13168161.118483hypothetical protein
Dester_1317112-3.309568hypothetical protein
Dester_1320113-2.692298hypothetical protein
Dester_1321013-2.906739ABC-2 type transporter
Dester_1322-112-2.395622Teichoic-acid-transporting ATPase
Dester_1323-111-2.530766outer membrane efflux protein
Dester_1324-210-1.707651type I secretion membrane fusion protein, HlyD
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1316RTXTOXINA414e-05 Gram-negative bacterial RTX toxin determinant A family...
		>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family

signature.
Length = 1024

Score = 40.7 bits (95), Expect = 4e-05
Identities = 28/132 (21%), Positives = 53/132 (40%), Gaps = 10/132 (7%)

Query: 441 GYASDVITGNSAGTTILLGSGNDNLQFVDGANAGESNIIKLGAGDDKL-------VVTTN 493
G +D + G + + G G+D Q + A N++ G G+DKL ++
Sbjct: 779 GDGNDKLIGVAGNNYLNGGDGDDEFQVQGNSLA--KNVLFGGKGNDKLYGSEGADLLDGG 836

Query: 494 NGYTYVFGEDGNDDVVINAIDTNDLV-DLGAGTDTLTLDNTNASTAVLRGVENLVIKARD 552
G + G GND + + ++ D G D L+L + + + N +I +
Sbjct: 837 EGDDLLKGGYGNDIYRYLSGYGHHIIDDDGGKEDKLSLADIDFRDVAFKREGNDLIMYKG 896

Query: 553 NGQAVTINAADS 564
G ++I +
Sbjct: 897 EGNVLSIGHKNG 908



Score = 32.2 bits (73), Expect = 0.016
Identities = 44/224 (19%), Positives = 70/224 (31%), Gaps = 23/224 (10%)

Query: 961 VKTATIALGGGTDKLDL--TNLANLDANGVGAVINLSSFTQTVDGVSVDAGKIVEFDGTD 1018
VK +++G T+K +++ + NL S + + D +F
Sbjct: 681 VKEQEVSVGKRTEKTQYRSYEFTHINGKNLTETDNLYSVEELIGTTRADKFFGSKFTDIF 740

Query: 1019 DGTNNITDGYTITVNDVEEIVGTKGADIIFAANTGTTITSEAGADKIVLGAGADKVIIAA 1078
G + + I G G D ++ T++ G D++ G G DK+I
Sbjct: 741 HGADG-----------DDLIEGNDGNDRLYGDKGNDTLSGGNGDDQLYGGDGNDKLI--- 786

Query: 1079 AGETGTITVADKDSSGDLSDGDTISGSFDVISNFEHGTDKLDISAVNDGTSTDTW-DSTN 1137
G G + D + + + G DKL S D D
Sbjct: 787 -GVAGNNYLNGGDGDDEFQVQG--NSLAKNVLFGGKGNDKLYGSEGADLLDGGEGDDLLK 843

Query: 1138 GLDANAYQIVLGTWDETTNTFTVDSGSGTDTMVLFDDGLSDVAV 1181
G N L + D G D + L D DVA
Sbjct: 844 GGYGNDIYRYLSGYGHHI---IDDDGGKEDKLSLADIDFRDVAF 884


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1322PF05272320.006 Virulence-associated E family protein
		>PF05272#Virulence-associated E family protein

Length = 892

Score = 31.6 bits (71), Expect = 0.006
Identities = 18/86 (20%), Positives = 28/86 (32%), Gaps = 30/86 (34%)

Query: 49 VLGIIGPNGAGKSTLLKILTGVIIPDEGKIHIDGRITGLLELGTGFNFEMTGIENIYLNG 108
+ + G G GKSTL+ L G+ D ++GTG +
Sbjct: 598 SVVLEGTGGIGKSTLINTLVGL------DFFSD----THFDIGTGKDSYE---------- 637

Query: 109 MLLGMTKEELDRKKDSIIEFSELGDF 134
+ G+ E SE+ F
Sbjct: 638 QIAGIV----------AYELSEMTAF 653


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1323PF01540320.007 Adhesin lipoprotein
		>PF01540#Adhesin lipoprotein

Length = 475

Score = 31.6 bits (71), Expect = 0.007
Identities = 23/99 (23%), Positives = 47/99 (47%)

Query: 313 YVSALKLEALELKKAAFEDLKQIKKEKSMQLDDSWHKLKQAEEKIKNLKRKLLNDYKIYK 372
++ + E E+KKA ++L +IK E +L + K+K+ +++ L K+ +
Sbjct: 208 WLEKIVSEWEEVKKAWSKELAEIKAEDDKKLAEENQKIKEGAKELLKLSEKIQSFADTIA 267

Query: 373 VIKKALEKGLKTEIDLKNQEVEIVRDKILLNEEVHTFST 411
+ LE+ + + K Q + + + EV TF+T
Sbjct: 268 LTITKLERKFQIDEKFKKQLISTIELLNKKSVEVKTFAT 306



Score = 29.7 bits (66), Expect = 0.028
Identities = 24/95 (25%), Positives = 43/95 (45%)

Query: 317 LKLEALELKKAAFEDLKQIKKEKSMQLDDSWHKLKQAEEKIKNLKRKLLNDYKIYKVIKK 376
+ E E+KKA ++L +IK E +L + K+K E++K + + K
Sbjct: 333 IVSEWEEVKKAWSKELAEIKAEDDKKLAEENQKIKNGVEELKKINNEAFELSKTVNKTIA 392

Query: 377 ALEKGLKTEIDLKNQEVEIVRDKILLNEEVHTFST 411
LEK K ++ K Q D + + ++ F+T
Sbjct: 393 ELEKKFKIDVSFKEQLKNFADDLLDKSRQIDEFTT 427


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1324RTXTOXIND2482e-79 Gram-negative bacterial RTX secretion protein D signat...
		>RTXTOXIND#Gram-negative bacterial RTX secretion protein D

signature.
Length = 478

Score = 248 bits (635), Expect = 2e-79
Identities = 89/428 (20%), Positives = 178/428 (41%), Gaps = 10/428 (2%)

Query: 33 KKYITFGSLVVFLIFGLLIGWAALAKIDTVVVAPGKVVVKSYKKPVQHKDWGTVTRIFVK 92
+ + + + + L +++ V A GK+ K ++ + V I VK
Sbjct: 54 SRRPRLVAYFIMGFLVIAFILSVLGQVEIVATANGKLTHSGRSKEIKPIENSIVKEIIVK 113

Query: 93 EGDFVKKGDPLLELEKLEQDTNYKVLESNYYNLLAERDRLLS-----EKKGLNHIAFSSE 147
EG+ V+KGD LL+L L + + +S+ E+ R E L + E
Sbjct: 114 EGESVRKGDVLLKLTALGAEADTLKTQSSLLQARLEQTRYQILSRSIELNKLPELKLPDE 173

Query: 148 FLQFKNKKLKEQIIRTQRELFFKRKRKLQSELAVLSERENQAKEQLKGLKSVLKIKENLL 207
+ + E+ + L ++ Q++ ++ + + + + + ENL
Sbjct: 174 PY---FQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLTVLARINRYENLS 230

Query: 208 NSYIKEIKEQEELVKEKLVSKIRLLDLMKEKERLEAEIKDIKSKIPQVLSQIEELNHQKT 267
+ + L+ ++ ++K +L+ + E++ KS++ Q+ S+I +
Sbjct: 231 RVEKSRLDDFSSLLHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSAKEEYQ 290

Query: 268 LQIENYQNEVASKLDEVLSKLSELKPKVIYAKEKVKKTIITANTSGQVLGLKIHAKGEVV 327
L + ++NE+ KL + + L ++ +E+ + ++I A S +V LK+H +G VV
Sbjct: 291 LVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAPVSVKVQQLKVHTEGGVV 350

Query: 328 KPGDTLMYIVPKKDEIFILAKVLPQDRDRVSKGQLVDLHFPAFLSIAANIVEGKVTYVAT 387
+TLM IVP+ D + + A V +D ++ GQ + AF + GKV +
Sbjct: 351 TTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEAFPYTRYGYLVGKVKNINL 410

Query: 388 DTLFDQATRHDYYETHIVLTDKGKEQLKKYGFNLVPGMPAVAYIKVEKVTPLEYVLQPVL 447
D + DQ + + + K L GM A IK + + Y+L P+
Sbjct: 411 DAIEDQRLGLVFNVIISIEENCLSTGNK--NIPLSSGMAVTAEIKTGMRSVISYLLSPLE 468

Query: 448 MLVKTAFK 455
V + +
Sbjct: 469 ESVTESLR 476


38Dester_1373Dester_1379N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_13730150.326128dTDP-glucose 4,6-dehydratase
Dester_13740151.406041protein of unknown function DUF820
Dester_13751141.192639DNA polymerase beta domain protein region
Dester_13760111.836719dTDP-4-dehydrorhamnose reductase
Dester_13770101.610154UDP-glucose 4-epimerase
Dester_1378-190.860171Oligosaccharyl transferase STT3 subunit
Dester_1379-2112.606518Mg chelatase, subunit ChlI
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1373NUCEPIMERASE1902e-60 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 190 bits (485), Expect = 2e-60
Identities = 73/331 (22%), Positives = 145/331 (43%), Gaps = 26/331 (7%)

Query: 1 MKLVVTGGAGFIGSEFVRQAVEKGLETVVVDKLTYAGDL----ERLKEVENK-ISFYKAD 55
MK +VTG AGFIG ++ +E G + V +D L D+ RL+ + F+K D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 56 ITNREFIEHIFKAEKPDVVVHWAAESHVDRSILDATPFIETNVKGTQILLDVAKETGVNL 115
+ +RE + +F + + V V S+ + + ++N+ G +L+ + +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 116 FVNIATDEVYGELGEDGQFYEDTPLN-PNSPYSVSKASADMLGRAYYRTYGLPVITVRPS 174
+ ++ VYG L F D ++ P S Y+ +K + +++ Y YGLP +R
Sbjct: 121 LLYASSSSVYG-LNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFF 179

Query: 175 NNYGYWQYPEKLIPVVILKALNNEPIPIYGTGENRREWLFVSDCAEAVFEIIE------- 227
YG W P+ + L + I +Y G+ +R++ ++ D AEA+ + +
Sbjct: 180 TVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADT 239

Query: 228 -----------KGKPGEIYNVGSGEERRNIDVVKSILQILNKPEDLITFVKDRPGHDFRY 276
P +YN+G+ +D ++++ L + +PG
Sbjct: 240 QWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAK-KNMLPLQPGDVLET 298

Query: 277 SLNTEKIEKELSWKAKVKFEEGIEKTVKWYL 307
S +T+ + + + + + ++G++ V WY
Sbjct: 299 SADTKALYEVIGFTPETTVKDGVKNFVNWYR 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1376NUCEPIMERASE473e-08 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 47.1 bits (112), Expect = 3e-08
Identities = 46/202 (22%), Positives = 76/202 (37%), Gaps = 29/202 (14%)

Query: 1 MRYLIFGAKGQLGREFVKWL--SGGLV-----------ESLKGKTVEWIGVGR---EECD 44
M+YL+ GA G +G K L +G V SLK +E + + D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 45 ISDLNQVLELFESTKPNVVVNCAAYNLVDKAEEDYVSAVKVNSVGVRNLAFACNRYR-AF 103
++D + +LF S V V + E+ + N G N+ C +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 104 LVHYSTDYVFDGKKENALYIEDDKPN-PLNEYGKSKLIGEEFIKEEIDNFLI----LRVS 158
L++ S+ V+ G + DD + P++ Y +K E + + LR
Sbjct: 121 LLYASSSSVY-GLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFF 179

Query: 159 WVYGE-GRQN-----FIYKLLK 174
VYG GR + F +L+
Sbjct: 180 TVYGPWGRPDMALFKFTKAMLE 201


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1377NUCEPIMERASE1882e-59 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 188 bits (478), Expect = 2e-59
Identities = 90/346 (26%), Positives = 150/346 (43%), Gaps = 43/346 (12%)

Query: 1 MKILVTGGAGYIGSHVVKALGEKGYKVLVVDNLSKGH----KEAVLYG------KLVVAD 50
MK LVTG AG+IG HV K L E G++V+ +DNL+ + K+A L + D
Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60

Query: 51 LEDKNTLDVIFKEFRPDAVMHFAAFIEVAQSLREPLKYYKNNTVNTINLLEVMLKNGVNK 110
L D+ + +F + V + V SL P Y +N +N+LE N +
Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120

Query: 111 FIFSSTAAVYGNPEKVPIPEIEPI-KPINPYGQSKAFVEKVLQDFDKSYGLKYVSLRYFN 169
+++S+++VYG K+P + + P++ Y +K E + + YGL LR+F
Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFT 180

Query: 170 AAGADPEGRIGESHDPETHLIPLILKTAKGEKESIKIFGTDYPTPDGTCIRDYIHVDDLA 229
G P GR P+ L +G +SI ++ G RD+ ++DD+A
Sbjct: 181 VYG--PWGR------PDMALFKFTKAMLEG--KSIDVYN------YGKMKRDFTYIDDIA 224

Query: 230 EAHILALEYLLNGGSS---------------EVFNCGYGHGFSVREVIDTARKVTGIDFK 274
EA I + + + + V+N G + + I GI+ K
Sbjct: 225 EAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAK 284

Query: 275 VEETERRPGDPAILVADSSKLRKVLDWKPKFDDLEYIIRTAWNWER 320
+PGD AD+ L +V+ + P+ ++ ++ NW R
Sbjct: 285 KNMLPLQPGDVLETSADTKALYEVIGFTPET-TVKDGVKNFVNWYR 329


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1379HTHFIS432e-06 FIS bacterial regulatory protein HTH signature.
		>HTHFIS#FIS bacterial regulatory protein HTH signature.

Length = 484

Score = 43.3 bits (102), Expect = 2e-06
Identities = 36/169 (21%), Positives = 55/169 (32%), Gaps = 42/169 (24%)

Query: 193 DIVGQ----YQAKRALEIAAAGHHNLFMIGPPGSGKTMLARRLPTIMPPMSEEEIVETTK 248
+VG+ + R L L + G G+GK ++AR
Sbjct: 138 PLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVAR------------------- 178

Query: 249 IYSVAGLFSEIPVVKRPFRAPHSGA-----SEVALIG-------GGASLKPGEVSLSHNG 296
L PF A + A E L G G + G + G
Sbjct: 179 -----ALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGG 233

Query: 297 VLFLDEMAEFKRSALEALRQPLEDGFVTISRASGTVTFPANFSLVAASN 345
LFLDE+ + A L + L+ G + G ++ +VAA+N
Sbjct: 234 TLFLDEIGDMPMDAQTRLLRVLQQG--EYTTVGGRTPIRSDVRIVAATN 280


39Dester_1442Dester_1453N        Y        NPathogenicity Island (unbiased-composition)
LocusTagDNBiasCDNBias%GCBiasProduct
Dester_1442114-1.741082N-methylation domain-containing protein
Dester_1443013-2.160889type II secretion system protein E
Dester_1444216-3.086815hypothetical protein
Dester_1445-118-4.202414hypothetical protein
Dester_1446-117-3.743381Tetratricopeptide TPR_1 repeat-containing
Dester_1447212-3.177660type II and III secretion system protein
Dester_1448212-3.223323hypothetical protein
Dester_1449210-2.113373hypothetical protein
Dester_1450210-1.438041hypothetical protein
Dester_1451210-1.036013Type II secretion system F domain
Dester_145239-1.034085chromosome segregation protein SMC
Dester_1453-29-0.094182UDP-glucuronate 5'-epimerase
ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1442BCTERIALGSPG413e-07 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 41.4 bits (97), Expect = 3e-07
Identities = 34/137 (24%), Positives = 57/137 (41%), Gaps = 33/137 (24%)

Query: 1 MKERKGFTLVELAIVLVIIGLLLGAV---LKGQELIQNAKYKKLINDLQGLSAAVYTYY- 56
+++GFTL+E+ +V+VIIG+L V L G + + A +K ++D+ L A+ Y
Sbjct: 4 TDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNK--EKADKQKAVSDIVALENALDMYKL 61

Query: 57 --DRY----------------KALPGDDPKAG-------DKWGSTYSNIINGDGNG--LI 89
Y L + K G D WG+ Y + G+ L+
Sbjct: 62 DNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADPWGNDYVLVNPGEHGAYDLL 121

Query: 90 SGSPTSTTNTDESVQIW 106
S P T++ + W
Sbjct: 122 SAGPDGEMGTEDDITNW 138


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1444BCTERIALGSPH369e-05 Bacterial general secretion pathway protein H signa...
		>BCTERIALGSPH#Bacterial general secretion pathway protein H

signature.
Length = 170

Score = 36.1 bits (83), Expect = 9e-05
Identities = 11/29 (37%), Positives = 21/29 (72%)

Query: 1 MKRSGFTLIEMAIILVILGLILGIGMGTM 29
M++ GFTL+EM +IL+++G+ G+ +
Sbjct: 1 MRQRGFTLLEMMLILLLMGVSAGMVLLAF 29


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1445BCTERIALGSPG342e-04 Bacterial general secretion pathway protein G signa...
		>BCTERIALGSPG#Bacterial general secretion pathway protein G

signature.
Length = 145

Score = 34.5 bits (79), Expect = 2e-04
Identities = 12/36 (33%), Positives = 23/36 (63%)

Query: 17 RKGFSLIEIAIVLVIVSILLGLGIRSCISGIETAKI 52
++GF+L+EI +V+VI+ +L L + + + E A
Sbjct: 7 QRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADK 42


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1446SYCDCHAPRONE412e-06 Gram-negative bacterial type III secretion SycD cha...
		>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD

chaperone signature.
Length = 168

Score = 40.7 bits (95), Expect = 2e-06
Identities = 25/129 (19%), Positives = 44/129 (34%)

Query: 206 LKIDPDYAEAYAGIGFLYLKLNSPKAAVIAFRRAHSLNPKEISYSVNLAISLLGSGNIDE 265
+I D E + F + + A F+ L+ + + + L G D
Sbjct: 29 NEISSDTLEQLYSLAFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDL 88

Query: 266 AILEFQKLKNKYPFLPEIYYNEAVAYLKKGYYKKAIEDFEIFLELTKANKFYEDYREEVL 325
AI + P ++ A L+KG +A + EL +++ V
Sbjct: 89 AIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQELIADKTEFKELSTRVS 148

Query: 326 KVLNQIKLI 334
+L IKL
Sbjct: 149 SMLEAIKLK 157



Score = 36.4 bits (84), Expect = 5e-05
Identities = 18/86 (20%), Positives = 35/86 (40%), Gaps = 1/86 (1%)

Query: 181 LYTYLGYAYTHLGRYTKALNAFKKALKIDPDYAEAYAGIGFLYLKLNSPKAAVIAFRRAH 240
LY+ + G+Y A F+ +D + + G+G + A+ ++
Sbjct: 39 LYSL-AFNQYQSGKYEDAHKVFQALCVLDHYDSRFFLGLGACRQAMGQYDLAIHSYSYGA 97

Query: 241 SLNPKEISYSVNLAISLLGSGNIDEA 266
++ KE + + A LL G + EA
Sbjct: 98 IMDIKEPRFPFHAAECLLQKGELAEA 123


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1447BCTERIALGSPD1614e-45 Bacterial general secretion pathway protein D signa...
		>BCTERIALGSPD#Bacterial general secretion pathway protein D

signature.
Length = 660

Score = 161 bits (409), Expect = 4e-45
Identities = 84/309 (27%), Positives = 139/309 (44%), Gaps = 39/309 (12%)

Query: 211 DKTASYSVEPISGTVIVTAKPETLKKVKEFIDTINGISDRQVLIEAKIVEVKLDKRNELG 270
DK + +IVTA P+ + ++ I ++ I QVL+EA I EV+ LG
Sbjct: 307 DKNIIIKAHGQTNALIVTAAPDVMNDLERVIAQLD-IRRPQVLVEAIIAEVQDADGLNLG 365

Query: 271 INW--KYLTFSNFLGSGGEYNTI---------------SFNSGAPEGKPFQLSIVKVNNT 313
I W K + F SG +T S S + N
Sbjct: 366 IQWANKNAGMTQFTNSGLPISTAIAGANQYNKDGTVSSSLASALSSFNGIAAGFYQGN-- 423

Query: 314 FSGLLGILSQFGKVNVLSSPRILAMNGQPAMIKVGRDYLAIYRTQTTSTTSTSSQTATTL 373
++ LL LS K ++L++P I+ ++ A VG++ T S T++ T+
Sbjct: 424 WAMLLTALSSSTKNDILATPSIVTLDNMEATFNVGQEV----PVLTGSQTTSGDNIFNTV 479

Query: 374 TTEEVTTNSILTEGVVLTIIPKIDDKGNIILNISPAISSLDSPLITGSTGETTDFINKVY 433
+ V G+ L + P+I++ +++L I +SS + + T+ +
Sbjct: 480 ERKTV--------GIKLKVKPQINEGDSVLLEIEQEVSS-----VADAASSTSSDLGAT- 525

Query: 434 SVNIRQLNTVVRVKNGQTVILGGLIAKSKSKEKEGVPILQDIPLLGNAFKSTSTISSKTE 493
N R +N V V +G+TV++GGL+ KS S + VP+L DIP++G F+STS SK
Sbjct: 526 -FNTRTVNNAVLVGSGETVVVGGLLDKSVSDTADKVPLLGDIPVIGALFRSTSKKVSKRN 584

Query: 494 LVIMLTPYV 502
L++ + P V
Sbjct: 585 LMLFIRPTV 593



Score = 43.8 bits (103), Expect = 1e-06
Identities = 27/182 (14%), Positives = 69/182 (37%), Gaps = 22/182 (12%)

Query: 77 SFDNIDLKKALLALGKATGYNVIVPPDIEGKVSIE----LKGESLKDSLNSLLKPFGYSY 132
SF D+++ + + K VI+ P + G +++ L E S+L +G++
Sbjct: 33 SFKGTDIQEFINTVSKNLNKTVIIDPSVRGTITVRSYDMLNEEQYYQFFLSVLDVYGFAV 92

Query: 133 KIDAKNIYVISKETKVFHINLPQTKRQFSSSIEASIGGSSEGTGSTTTTSTATMSIGNSY 192
+ + + +K ++++ + ++ G G T ++ +
Sbjct: 93 INMNNGVLKVVR-----------SKDAKTAAVPVA-SDAAPGIGDEVVTRVVPLTNVAAR 140

Query: 193 DLDIWNNIKSSIDVIVKNDKTASYSVEPISGTVIVTAKPETLKKVKEFIDTINGISDRQV 252
DL + + N S S +++T + +K++ ++ ++ DR V
Sbjct: 141 DL------APLLRQLNDNAGVGSVVHYEPSNVLLMTGRAAVIKRLLTIVERVDNAGDRSV 194

Query: 253 LI 254
+
Sbjct: 195 VT 196


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1451BCTERIALGSPF1863e-57 Bacterial general secretion pathway protein F signa...
		>BCTERIALGSPF#Bacterial general secretion pathway protein F

signature.
Length = 408

Score = 186 bits (475), Expect = 3e-57
Identities = 93/405 (22%), Positives = 186/405 (45%), Gaps = 5/405 (1%)

Query: 1 MARYKVTFLSQDGIVNTEIVEAKNESELFSLFSERDVILLEYKKDWFSFLKEFSLLDLFQ 60
MA+Y L G EA + + L ER ++ L ++ K S +
Sbjct: 1 MAQYHYQALDAQGKKCRGTQEADSARQARQLLRERGLVPLSVDENRGDQQKSGSTGLSLR 60

Query: 61 R-RKISKQELADFCFYFGRALDMGISVLEILEDIGKSSKNKYFRKVMETLRERVTAGSSL 119
R ++S +LA + + + E L+ + K S+ + ++M +R +V G SL
Sbjct: 61 RKIRLSTSDLALLTRQLATLVAASMPLEEALDAVAKQSEKPHLSQLMAAVRSKVMEGHSL 120

Query: 120 SEAME-LTGAFPPELIGLVKVGESTDALPKVFLNYAEYLDWVISIEKEVKQALSYPIFVS 178
++AM+ G+F +V GE++ L V A+Y + + ++QA+ YP ++
Sbjct: 121 ADAMKCFPGSFERLYCAMVAAGETSGHLDAVLNRLADYTEQRQQMRSRIQQAMIYPCVLT 180

Query: 179 FIMVFTIAIMFGYIIPQIIPAITAMGLKEYPLPTKILLWSGKYVQIFWKEIVITPILLVI 238
+ + ++I+ ++P+++ M + PL T++L+ V+ F +++ + +
Sbjct: 181 VVAIAVVSILLSVVVPKVVEQFIHMK-QALPLSTRVLMGMSDAVRTFGPWMLLALLAGFM 239

Query: 239 FFKLIMRYSIKARYIWDRLKISFPLIGDIFQKASLSRDMRALAEVYRSGGTILRALDIII 298
F++++R K R + R + PLIG I + + +R R L+ + S +L+A+ I
Sbjct: 240 AFRVMLRQE-KRRVSFHRRLLHLPLIGRIARGLNTARYARTLSILNASAVPLLQAMRISG 298

Query: 299 NHVEQNLYIKSIFQKVKENIMVGDMLSVAMERSGFFQSTIIRMIKLGEETGALDKSLLRL 358
+ V N Y + + + G L A+E++ F + MI GE +G LD L R
Sbjct: 299 D-VMSNDYARHRLSLATDAVREGVSLHKALEQTALFPPMMRHMIASGERSGELDSMLERA 357

Query: 359 AEIYEDDMRRKIQTMTVVIEPTLQLVLGGILGIVALGILLPVYNI 403
A+ + + ++ + EP L + + ++ + L IL P+ +
Sbjct: 358 ADNQDREFSSQMTLALGLFEPLLVVSMAAVVLFIVLAILQPILQL 402


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1452GPOSANCHOR642e-12 Gram-positive coccus surface protein anchor signature.
		>GPOSANCHOR#Gram-positive coccus surface protein anchor signature.

Length = 539

Score = 63.9 bits (155), Expect = 2e-12
Identities = 42/296 (14%), Positives = 106/296 (35%)

Query: 202 RTLKSQAEKAKKFQELRNLEKELELKLLGLQLKNLRLEKEVSENSLKLLQEDRISLEREV 261
+ + R + E+E L L+ +L + ++ L E+ + + ++
Sbjct: 42 AVATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKL 101

Query: 262 SVLQVELEELRKELESITKEIEETSQELYEVEKSKKEASVKREFLQKEIKRLEEELKEKT 321
L E +++ + + + L S K + L+ E L +
Sbjct: 102 RKNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLE 161

Query: 322 FEREHKIRKIESIRKELQLIFQEESELQKKLEDLENREKEKERIVKELQQKRKAFEERLK 381
E + + +++ + E++ L+ + +LE + K K E
Sbjct: 162 KALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKA 221

Query: 382 ELKSSLSTTSTQISKLQLDMAREEERFKSLKNIKEKLPGEIEKLQKEKEYYLSYIERFVE 441
L + + + + + K+L+ K L +L+K E +++
Sbjct: 222 ALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSA 281

Query: 442 KENSLKEKIEKLKEELENLKKEKKELLERLEVINSEVSEKREEIVSLRSKIESIEK 497
K +L+ + L+ E +L+ + + L + + ++ RE L ++ + +E+
Sbjct: 282 KIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEE 337



Score = 59.7 bits (144), Expect = 5e-11
Identities = 44/326 (13%), Positives = 103/326 (31%), Gaps = 14/326 (4%)

Query: 168 SFKEKKEETLQKLSEAEQNLESVRSVIDEVGKNLRTLKSQAEKAKKFQELRNLEKELELK 227
+ +TL+K+ E E + + +L + + +L+
Sbjct: 43 VATRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLR 102

Query: 228 LLGLQLKNLRLEKEVSENSLKLLQEDRISLEREVSVLQVELEELRKELESITKEIEETSQ 287
L + + E L++ + +++ L E ++ + +
Sbjct: 103 KNDKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEK 162

Query: 288 ELYEVEKSKKEASVKREFLQKEIKRLEEELKEKTFEREHKIRKIESIRKELQLIFQEESE 347
L S K + L+ E LE E + K L+ +
Sbjct: 163 ALEGAMNFSTADSAKIKTLEAEKAALEARQAE--------------LEKALEGAMNFSTA 208

Query: 348 LQKKLEDLENREKEKERIVKELQQKRKAFEERLKELKSSLSTTSTQISKLQLDMAREEER 407
K++ LE + +L++ + + + T + + L+ A E+
Sbjct: 209 DSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 268

Query: 408 FKSLKNIKEKLPGEIEKLQKEKEYYLSYIERFVEKENSLKEKIEKLKEELENLKKEKKEL 467
+ N +I+ L+ EK + + L + L+ +L+ ++ KK+L
Sbjct: 269 LEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQL 328

Query: 468 LERLEVINSEVSEKREEIVSLRSKIE 493
+ + + SLR ++
Sbjct: 329 EAEHQKLEEQNKISEASRQSLRRDLD 354



Score = 57.8 bits (139), Expect = 2e-10
Identities = 64/366 (17%), Positives = 130/366 (35%), Gaps = 15/366 (4%)

Query: 655 KNSLLEMEKELENLKEKLTREEKVLSCLQSKVLPIREEIDEKEEGIDSLKEAIQQKKMEL 714
SL E +++ L+ + EK L + +I E +L + L
Sbjct: 105 DKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKAL 164

Query: 715 FEVGSKIKEARRKLADLERKENELEDKLKRAVESINSYNSRKDIFLQKIESLSKKKDELV 774
+ K+ LE ++ LE + +++ + KI++L +K L
Sbjct: 165 EGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALA 224

Query: 775 KEIEKLEEDIKKLEANIGIEKEELSKYVSKKILLAEKLKNLKERKESKERFVRTLQKEIE 834
LE+ ++ + ++ ++K L + L++ E F +I+
Sbjct: 225 ARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIK 284

Query: 835 EIEKRIEKDEENLKKAAIGVKRAEEILGGVDESIDEIKKELQLLEERRGEITSMVKTKEE 894
+E E E ++ + ++++L E + ++ + + EE
Sbjct: 285 TLEAEKAALEAEKAD-------LEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEE 337

Query: 895 ALKSKNKDLSEVQNKLKETEVAVARFNVKEEEIISKILELEKSVSDALEAALAA---GSE 951
K ++ L + A + + ++ LE + +S+A +L S
Sbjct: 338 QNKISEASRQSLRRDLDASREAKKQLEAEHQK-----LEEQNKISEASRQSLRRDLDASR 392

Query: 952 EEVKKELINLKEKISKIGNVNFLAIEEYEKVKERYGFILEQEKDLIESIKNLREAIRKLD 1011
E K+ L+E SK+ + L E E K E + L K L+E + K
Sbjct: 393 EAKKQVEKALEEANSKLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQA 452

Query: 1012 EEIEKK 1017
EE+ K
Sbjct: 453 EELAKL 458



Score = 52.0 bits (124), Expect = 1e-08
Identities = 43/285 (15%), Positives = 98/285 (34%), Gaps = 14/285 (4%)

Query: 644 TGTGSIVGKFKKNSLLEMEKELENLKEKLTREEKVLSCLQSKVLPIREEIDEKEEGIDSL 703
T + + + + + E E LK K + L+ + EE+ +E +
Sbjct: 45 TRSQTDTLEKVQERADKFEIENNTLKLKNSDLSFNNKALKDHNDELTEELSNAKEKLRKN 104

Query: 704 KEAIQQKKMELFEVGSKIKEARRKLADLERKENELEDKLKRAVESINSYNSRKDIFLQKI 763
+++ +K ++ E+ ++ + + L K+K + +RK + +
Sbjct: 105 DKSLSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKAL 164

Query: 764 ESLSKKKDELVKEIEKLEEDIKKLEANIGI--------------EKEELSKYVSKKILLA 809
E +I+ LE + LEA + ++ ++K LA
Sbjct: 165 EGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALA 224

Query: 810 EKLKNLKERKESKERFVRTLQKEIEEIEKRIEKDEENLKKAAIGVKRAEEILGGVDESID 869
+ +L++ E F +I+ +E E + ++ A I
Sbjct: 225 ARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIK 284

Query: 870 EIKKELQLLEERRGEITSMVKTKEEALKSKNKDLSEVQNKLKETE 914
++ E LE + ++ + +S +DL + K+ E
Sbjct: 285 TLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLE 329



Score = 47.8 bits (113), Expect = 2e-07
Identities = 54/351 (15%), Positives = 119/351 (33%), Gaps = 17/351 (4%)

Query: 144 QIDRVLKMKPQERRLLIDEAAGITSFKEKKEETLQKLSEAEQNLESVRSVIDEVGKNLRT 203
+++ L+ + + + K L +A + + + K L
Sbjct: 124 DLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEA 183

Query: 204 LKSQAEKAKKFQELRNLEKELELKLLGLQLKNLRLEKEVSENSLKLLQEDRISLEREVSV 263
K+ E + E ++K L EK L++ +
Sbjct: 184 EKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTA 243

Query: 264 LQVELEELRKELESIT-------KEIEETSQELYEVEKSKKEASVKREFLQKEIKRLEEE 316
+++ L E ++ K +E K ++ L+ E LE +
Sbjct: 244 DSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQ 303

Query: 317 LKEKTFEREHKIRKIESIRKELQLIFQEESELQKKLEDLENREKEKERIVKELQQKRKAF 376
+ R+ R +++ R+ + + E +L+++ + E + R + ++ +K
Sbjct: 304 SQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL 363

Query: 377 EERLKELKSSLSTTSTQISKLQLDMAREEERFKSLKNIKEKLPGEIEKLQKEKEYYLSYI 436
E ++L+ + L+ D+ E K ++ E+ ++ L+K
Sbjct: 364 EAEHQKLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLN------- 416

Query: 437 ERFVEKENSLKEKIEKLKEELENLKKEKKELLERLEVINSEVSEKREEIVS 487
E E S K ++ E L+ E K L E+L E+++ R S
Sbjct: 417 ---KELEESKKLTEKEKAELQAKLEAEAKALKEKLAKQAEELAKLRAGKAS 464



Score = 47.8 bits (113), Expect = 2e-07
Identities = 46/284 (16%), Positives = 108/284 (38%)

Query: 654 KKNSLLEMEKELENLKEKLTREEKVLSCLQSKVLPIREEIDEKEEGIDSLKEAIQQKKME 713
+ +E E L + EK L + +I E +L+ + +
Sbjct: 139 DSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 198

Query: 714 LFEVGSKIKEARRKLADLERKENELEDKLKRAVESINSYNSRKDIFLQKIESLSKKKDEL 773
L + K+ LE ++ L + +++ + KI++L +K L
Sbjct: 199 LEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAAL 258

Query: 774 VKEIEKLEEDIKKLEANIGIEKEELSKYVSKKILLAEKLKNLKERKESKERFVRTLQKEI 833
+LE+ ++ + ++ ++K L + +L+ + + ++L++++
Sbjct: 259 EARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDL 318

Query: 834 EEIEKRIEKDEENLKKAAIGVKRAEEILGGVDESIDEIKKELQLLEERRGEITSMVKTKE 893
+ + ++ E +K K +E + +D ++ + LE ++ K E
Sbjct: 319 DASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISE 378

Query: 894 EALKSKNKDLSEVQNKLKETEVAVARFNVKEEEIISKILELEKS 937
+ +S +DL + K+ E A+ N K + ELE+S
Sbjct: 379 ASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEES 422


ORFs having significant similarity with Known Virulence factors
LocusTagHitsScoreE-valueComments
Dester_1453NUCEPIMERASE391e-139 Nucleotide sugar epimerase signature.
		>NUCEPIMERASE#Nucleotide sugar epimerase signature.

Length = 334

Score = 391 bits (1006), Expect = e-139
Identities = 124/329 (37%), Positives = 196/329 (59%), Gaps = 21/329 (6%)

Query: 1 MKTVFLTGAAGFIGYKTAEILIQKGYNVIGVDNLNNYYDVRLKEYRLKNLEKNKNFKFYQ 60
MK +TGAAGFIG+ ++ L++ G+ V+G+DNLN+YYDV LK+ RL+ L + F+F++
Sbjct: 1 MK-YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQ-PGFQFHK 58

Query: 61 VDIENFGALKVIFEDNKIDGVINLAARAGVRYSLIDPFVYVRTNTTGTLNLLELMKDKKV 120
+D+ + + +F + V R VRYSL +P Y +N TG LN+LE + K+
Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118

Query: 121 KKFVLASTSSLY-AGQPMPFKEDLPVNTPISPYAASKKGAEAVAYSYHYLYGIDVTVLRY 179
+ + AS+SS+Y + MPF D V+ P+S YAA+KK E +A++Y +LYG+ T LR+
Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRF 178

Query: 180 FTVYGPIGRPDMSIFRFIKWIDEGKPIEIFGDGTQSRDFTYIDDIAKGTVKALETET--- 236
FTVYGP GRPDM++F+F K + EGK I+++ G RDFTYIDDIA+ ++ +
Sbjct: 179 FTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHAD 238

Query: 237 ---------------GYEIINLGGNKPYELNYVIELIEEYIGKKAEKIYKPFHKADLKAT 281
Y + N+G + P EL I+ +E+ +G +A+K P D+ T
Sbjct: 239 TQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIEAKKNMLPLQPGDVLET 298

Query: 282 WADIEKAKRILDWEPQIPLEEGLKKTVDW 310
AD + ++ + P+ +++G+K V+W
Sbjct: 299 SADTKALYEVIGFTPETTVKDGVKNFVNW 327



 
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