Figures

1 EcoRI g|aattc2 1 gaattctatgaaaatgtctaaagaaaatggggaaacaatttcaaa 46 aaggcacagtttcaatggtttccgaattatactaaatccctctaa 91 EcoRV gat|atc110 91 MspI c|cgg98 91 HpaII c|cgg98 91 aaacttccggcaaattgatatccgtaaaagagcaaatccgcattt 136 MspI c|cgg172 136 HpaII c|cgg172 136 ttgccgaaaattaaaatttccgacaaatcggcaaaccggcaattt 181 MspI c|cgg193 181 HpaII c|cgg193 181 ggcgaaatttgccggaacgattgccgcccacccctgttccagagg 226 AluI ag|ct244 226 ttcaaactggtagcaaagctcaaaatttctcaaattctccaattt 271 RsaI gt|ac292 271 ttttttgaattttggcagtgtaccaaaatgacattcagtcatatt 316 HinfI g|antc353 316 ggtttattatagatttatttagataaaatcctaaatgattctacc 361 DraI ttt|aaa379 361 DraI ttt|aaa364 HinfI g|antc389 361 tttaaagatgcccactttaaaagtaatgactcaaacttcaaattg 406 HinfI g|antc413 406 ctctaagattctattgaattaccatcttttcctctcattttctct 451 MboII n|nnnnnnntcttc482 451 cactgtctatttcatcacaaattcatccctctctcctctcttctc 496 tctccctctctctctctttctctttgctcatcatctgtcattttg 541 tccgttcctctctctgcgccctcagcgttccccacactctctcgc 586 MboII n|nnnnnnntcttc609 586 AatII gacgt|c604 586 MboII n|nnnnnnntcttc596 586 ttctcttttcctagacgtcttcttttttcatcttcttcagccttt 631 VspI at|taat675 631 ttcgccattttccatctctgtcaatcattacggacgacccccatt 676 aatttttggatgtgctctgcgagcaagagcacacggaactcattt 721 attttttgttggaatttctttttcattttagttagttaaaaggtt 766 RsaI gt|ac801 766 attcagtgcaaaaattgattttttgctcttttggtacattagagg 811 MspI c|cgg841 811 HpaII c|cgg841 811 AccIII t|ccgga840 811 ggtttgaaagttttccataaaaagggtttccggagtaaaaaatct 856 aaaaatttttacaaacatttccaaaaattttgggaacatatcttt 901 VspI at|taat945 901 tcaaaaactcaaaaaaaaacgcaaacatcataaatttaatcaatt 946 SspI aat|att975 946 DraI ttt|aaa969 946 SspI aat|att964 946 aattggatgcgttcaaatattttaaaaatatttttgaattgcata 991 VspI at|taat1015 991 tttctttactcataaaaaaattattaatgtttaagtaaaaaaaat 1036 DraI ttt|aaa1049 1036 SspI aat|att1036 1036 atttcaggattttaaacaaaaactgtaaatcccataaaacagtgt 1081 tattttttagttatttttagtaattatttgtgaattaaaaacaca 1126 SspI aat|att1140 EcoRI g|aattc1168 1126 ttttttttcaaaatattccttgaataaataaaattgaaagtgaat 1171 SspI aat|att1178 1171 tcaaaatatttcaccaattttacaaaatttgaaaatttgaaaaaa 1216 SspI aat|att1217 SspI aat|att1245 1216 tattttctaccaaattgtgctcaaaaaatattattaagttcccag 1261 SspI aat|att1286 1261 gaaatttatgatgaaaacttggaatatttttggtaaaattttctc 1306 aaaaaattgaaagttgtcaaaaacacactaacactatacctcgcg 1351 DraI ttt|aaa1391 1351 DraI ttt|aaa1381 1351 gcatagaaaaaactggtggccgaattttttaaaccaatttaaatt 1396 RsaI gt|ac1417 1396 aaatatatttttgcactttgtacaaaaaaatttgaagttcccagg 1441 SspI aat|att1457 1441 aaattcatacggaaatattttccagaacttttataatttttattt 1486 taattctcaaaatttacaataaaaacttggaaacctgaaatttgc 1531 ttaaagaataaccctatcccccacggcctagaaaatactggtggc 1576 cgaattttttccgcagccacaccacctctaatctccagagcactc 1621 AluI ag|ct1653 1621 tccaaaattcccataatccccaaaattttcagctacaacagcata 1666 MspI c|cgg1702 1666 HpaII c|cgg1702 1666 PvuI cgat|cg1696 1666 NdeII |gatc1693 1666 MboI |gatc1693 1666 TaqI t|cga1692 1666 ClaI at|cgat1692 1 M K A E Q Q Q Q S I A P G S A 1666 tATGAAGGCAGAGCAACAACAACAATCGATCGCACCCGGCTCGGC 1711 MboII gaagannnnnnnn|1745 16 T F P S Q 1711 AACCTTCCCGTCTCAGgtgaggaagacgcagagaaatgagagaga 1756 EcoRV gat|atc1792 1756 gagcgtgtttatgcacgcagtatactaactcctgatatccaattt 1801 MboII n|nnnnnnntcttc1820 1801 TaqI t|cga1808 1801 ClaI at|cgat1808 1801 catatatcgatatgcttgctttcttttcttctacgaagaaattgt 1846 MboII gaagannnnnnnn|1848 1846 ttgaacttttctcaaaaaacgcggattttagagagacttagagtt 1891 HinfI g|antc1933 1891 gctccaaataaatttaatttgcaattttgcacttactattagaat 1936 DraI ttt|aaa1980 1936 ctacttttatagcgctaaaatgaccgaataacacagtagatttta 1981 DraI ttt|aaa1993 1981 aaagaaaattttaaaaattatttttttgtgcagtcaaatttcgct 2026 agtttttgccactcatttttgaacatttgtatatgtgcctttcgg 2071 tggttcaatcaggggtggcacgtggtgtcagagtgttccatttcg 2116 NdeII |gatc2130 2116 MboI |gatc2130 2116 NdeII |gatc2120 2116 MboI |gatc2120 2116 gtttgatctacgttgatctacaaaaaatgcgggagaagagacgca 2161 MboII gaagannnnnnnn|2163 2161 gagttctcaactgatttcacatggttatgaaccaggggtggttgg 2206 AluI ag|ct2226 2206 caaatttgccgtttgccgagctgggcaaattttgagatttgccgc 2251 AatII gacgt|c2278 2251 acaccccttttatgaacgtgctgacgtcacatctttttggacaaa 2296 EcoRI g|aattc2297 2296 gaattcccgcattttttgtatatcaaaccgtgatgggacagcctg 2341 gcaccacgtggggttggcggcaaatgattttttccgacaaatcgg 2386 MspI c|cgg2402 2386 HpaII c|cgg2402 2386 ctttaaggcaaattgccggaacccaaagtttctggcaaattgttg 2431 atttgccgaaaaaaaatttcaaaaaggcacagttttaagtgtttc 2476 MspI c|cgg2506 2476 AccIII t|ccgga2505 2476 SspI aat|att2489 HpaII c|cgg2506 2476 cgtcttataaaatatttctctaaaaatttccggaaaacggaaaat 2521 MspI c|cgg2524 2521 HpaII c|cgg2524 2521 tgccggaaatacaaagtttcgtcaaatcggcaaacctgcaatttt 2566 AluI ag|ct2605 2566 gccgaaccaactgccgccaacacctggttcccaaaaaagctgcac 2611 SspI aat|att2620 2611 tgtttgaatattaaaaaaaaaattctctcaaaaataataaattgt 2656 SphI gcatg|c2696 2656 RsaI gt|ac2682 2656 gaaataggctaaattcacatctatgtacaaaaattgcatgccagc 2701 MspI c|cgg2703 2701 HpaII c|cgg2703 2701 accggtcaattttctcaattactttttgtctatttttgccgaaca 2746 taatttgctgtttccttggcagaatacagaaaactcttttttttt 2791 MspI c|cgg2811 2791 HpaII c|cgg2811 21 M P R P P P V T E Q A I T T 2791 tcagATGCCCCGACCCCCACCGGTAACCGAACAGGCTATAACCAC 2836 NruI tcg|cga2853 2836 AluI ag|ct2846 MboII n|nnnnnnntcttc2880 35 E A E L L A K N Q I T P N D V 2836 CGAGGCGGAGCTTCTCGCGAAAAATCAAATTACACCAAATGATGT 2881 HinfI g|antc2896 2881 AluI ag|ct2886 2881 NheI g|ctagc2882 SspI aat|att2917 50 L A L P G I T Q G 2881 GCTAGCTCTTCCAGGAATCACTCAAGgtaagtgaatatttgctct 2926 RsaI gt|ac2945 2926 attgataaacgcgccgtgtactccacgtggacaaacatatcaaat 2971 HinfI g|antc2978 59 F L C S P S A N V Y N I E 2971 tttcagGATTCCTATGCTCCCCATCGGCAAACGTCTATAACATTG 3016 MboII n|nnnnnnntcttc3054 3016 TaqI t|cga3044 72 F T K F Q I R D L D T E H V L 3016 AGTTCACCAAATTCCAAATCCGTGACCTCGACACGGAACACGTGC 3061 MspI c|cgg3079 3061 HpaII c|cgg3079 3061 TaqI t|cga3065 HinfI g|antc3099 87 F E I A K P E N E T E E N L Q 3061 TCTTCGAAATTGCAAAACCGGAAAATGAGACGGAAGAGAATCTGC 3106 HinfI g|antc3118 TaqI t|cga3141 3106 PstI ctgca|g3107 ClaI at|cgat3141 3106 MboII gaagannnnnnnn|3106 EcoRV gat|atc3139 102 A Q A E S A R Y V R Y R F A P 3106 AGGCGCAAGCCGAATCGGCAAGATATGTCCGATATCGATTTGCGC 3151 TaqI t|cga3188 117 N F L K L K T V G A T V E F K 3151 CGAATTTTCTGAAATTAAAGACGGTCGGCGCGACGGTCGAGTTCA 3196 TaqI t|cga3225 3196 NdeII |gatc3212 3196 MboI |gatc3212 132 V G D V P I T H F R M I E R H 3196 AGGTCGGCGATGTGCCGATCACACATTTTCGAATGATTGAACGGC 3241 NdeII |gatc3252 3241 MboI |gatc3252 147 F F K D R L L K C F D F E F G 3241 ACTTTTTCAAGGATCGTTTATTGAAATGCTTTGATTTTGAATTTG 3286 HinfI g|antc3287 TaqI t|cga3304 162 F C M P N S R N N C E H I Y E 3286 GATTCTGTATGCCAAATTCGAGAAACAACTGTGAACATATCTATG 177 F P Q L S Q Q L M 3331 AATTTCCACAACTTTCACAACAACTCAgtgagtttgaagcgggaa 3376 SacI gagct|c3390 3376 AluI ag|ct3388 3376 aaattatttgagctcattttgtcaatagagcgaattttctaggtt 3421 SspI aat|att3439 3421 DraI ttt|aaa3424 AluI ag|ct3451 3421 tttaaaaataaaataaatattgacaaaaagctgccctacagtagt 3466 TaqI t|cga3493 3466 tcaaaatgtgaaaacttggaggaaattcgagttttctctgagaag 3511 tttttggcgggtaattcaaattttctgagaaaaaaattagccgcg 3556 EcoRI g|aattc3558 3556 agaattcaaattttctgagaaaattatttgacgggaaattcaaaa 3601 tttctcggaaatgtattgaacatttgagaaattttgttaaaaact 3646 acattaaaccgataaaaaagttcgcaaattttttttaagtgctca 3691 MboII gaagannnnnnnn|3713 3691 EcoRI g|aattc3709 3691 TaqI t|cga3699 3691 tggtgcatcgaagagaagaattcttgccaaaactagcccatgaaa 3736 ttaaaaattatcgcggaaaaaaactgactaattggaaagtaaaat 3781 gaaatttgcaaaaaaaaaatatgtttttttgttgtaaaattaaaa 3826 NdeII |gatc3856 3826 MboI |gatc3856 3826 BclI t|gatca3856 186 D D M I N N P N 3826 aaaaaactgtatattttcagTGGACGACATGATCAATAATCCAAA 3871 AluI ag|ct3889 AluI ag|ct3911 194 E T R S D S F Y F V E N K L V 3871 CGAGACCCGCTCCGATAGCTTCTATTTTGTGGAGAATAAGCTCGT 209 M H N K A D Y S Y D A * 3916 AATGCACAATAAGGCCGACTACTCGTATGATGCATAAtttcccgc 3961 cgatggaataaaactaataatttttctcgcaatttatagaaatca 4006 DraI ttt|aaa4030 4006 tgtgattttcctaccgctaaatttaaaatttcctaatttttcact 4051 AluI ag|ct4093 4051 atatttctcacaaaatgcattccgaaaactctcatttttcagctt 4096 ttcaatacaaatcccaataaatgatttttcaaatataatgttctc 4141 aattgatatgttttatttgaatgttggaaaaaaccataaaattaa 4186 aaaaaacttgaattattcaaaaataattcggcccttttacaaaaa 4231 attatcataaaaattaaatttggaaaatctgtgatttgtcgcttt 4276 MspI c|cgg4278 MspI c|cgg4294 4276 HpaII c|cgg4278 HpaII c|cgg4294 AluI ag|ct4320 4276 gccggaagtttccaattccggcaactaaccgatttgccgattagc 4321 MspI c|cgg4363 4321 HpaII c|cgg4363 4321 tagaaatttcaattccgacaatgtgccgctttgccgatttgccgg 4366 MspI c|cgg4381 4366 HpaII c|cgg4381 AluI ag|ct4407 4366 aaatttccaatttaccggcaattaacggacttgccgattagctag 4411 aaatttaactttagataaacttctcaacaaattcaaattggaaag 4456 MspI c|cgg4477 MspI c|cgg4493 4456 HpaII c|cgg4477 HpaII c|cgg4493 4456 ttcagcgatttgccgcttgaccggaagtttcagttaccggacttc 4501 agccattgcgattgcaaagtttactgcaaatgcgcatg

A restriction map generated using Rest and Trans Map. The translation (shown in blue) was generated using the uppercase option, which treats the uppercase text as a continuous reading frame (use GenBank Feature Extractor to automatically convert exons to uppercase before using Rest and Trans Map). Each restriction site label (shown in red) is aligned with the first base after the cleavage site on the direct strand.

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