.. _getting-started: Getting Started Guide ===================== This guide is intended as a reference for those using VLinux for the first time. Instructions are provided for download and testing. There are also notes on accessing the various programs that come with the latest release. .. toctree:: :maxdepth: 2 Download -------- The live DVD iso image can be downloaded from the :ref:`download` page Create the DVD -------------- The iso file should be burnt as an **image** using your DVD writing software. If you are using Linux, you can use K3B or Brasero. On Windows you can use `InfraRecorder `_ .. note:: When you burn the image, please use the *disc-at-once* option instead of *track-at-once* Brasero ....... .. figure:: images/brasero-burn-image.png :alt: Write iso image using Brasero InfraRecorder ............. .. figure:: images/infrarecorder-burn-image.png :alt: Write iso image using InfraRecorder Use the **Write Image** option in InfraRecorder to burn the iso Boot from DVD ------------- With the DVD in the drive, restart your computer and choose to boot from DVD instead of the hard disk. On most systems, this can be done by using a special key like **F9** or **F12**. This will be displayed at boot. If this is not the case, modify the boot order in the BIOS by entering setup (**F2** or **DEL** mostly). .. note:: This process does not change existing data on your hard disk. If you change the boot order in the BIOS, you can always revert it to the default. Once the booting from DVD is successful, the login screen would be displayed. Logging in ---------- A default user has already been setup in the appliance. The login details for user and the root user/Administrator is below .. csv-table:: :header: "User", "Username", "Password" Default user, vlinux, vlinux Administrator, root, linux Test some applications ---------------------- Here are some applications you would want to try after logging in EMBOSS ...... Launch Firefox and visit http://localhost/emboss. This will present the emboss-explorer interface to EMBOSS. You can test some programs by selecting them from the panel on the left. .. note:: The first time when Firefox is launched, there will be a prompt to setup Zotero. Please accept the defaults here to complete the setup. Sequence Manipulation Suite ........................... Launch Firefox and visit http://localhost/sms2 NCBI BLAST .......... Launch Firefox and visit http://localhost/blast. You can then choose `Regular BLAST without client-server support`. Try doing a BLAST searching using the sequence below :: CCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCA TCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGT AGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAG GTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACACAACGT GAGCGTCGAACAATTTTTCGTATAACGTCTTAGCCATGATTACACCCCTTCTGCTACATAGCGGGCAATG ATATCGCCCATTTCATCGGTACTAACGGCGGCAGCGCCACGGGCTAAATCCCCGGTGCGAATGCCTTCTT Rasmol ...... Open GNOME Terminal found under `More Applications` in the main menu and then type ``rasmol``. This will launch Rasmol. Load a PDB file using :menuselection:`File --> Open`. This will prompt for a PDB file name in GNOME Terminal from where RasMol was launced. You can try loading the PDB file below as an example :: /usr/share/python-biopython/Tests/PDB/1A8O.pdb ClustalX ........ Open GNOME Terminal and type ``clustalx2``. Use the :menuselection:`Load sequences` to load sequences from file. You can * download a multi-fasta format file or * copy the example file :file:`/usr/share/doc/packages/python-biopython/Doc/examples/opuntia.fasta` from the BioPython package to /home/vlinux Do an alignment using :menuselection:`Alignment --> Do Complete Alignment`