cluster # 10853

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Schistosoma mansoni
cluster # 10853 cluster # 10853 Sequences # 23 consensus # 5

see consensus multiple alignment with clustalw

Direct access to contigs :

consensus_10853#0 length = 540 sequences # 1
consensus_10853#1 length = 401 sequences # 16

consensus_10853#2 length = 284 sequences # 4
consensus_10853#3 length = 136 sequences # 1
consensus_10853#4 length = 89 sequences # 1

consensusID : consensus_10853#0
NCBI blastX ! send the sequence to the NCBI site !
NCBI blastN ! send the sequence to the NCBI site !
Sequences nbr = 1
consensus length = 540
fasta sequence

                              [TCCCACTTCGTTTCCCACTTAGCCAATATTAGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGTGTCCGGACGTTAGCACCCGGTGCACTGTCTCCCAAGCTGTACTCATCGGTATTCGGAGTTTGCATAGGTTTGGTAAGTCGCCATGACCCCCTAGCCTAAACAGTGCTCTACCCCCGACGGTAATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATCTCCAGGTTAGCTCAGCCGGGTGTTGGATGTCCCGGTTCAAGCCTGTAGTCGTGCCTGGTAGGCAAATCCGCCGGGCTTAGATGAGGGGTGATAACGAGGCGGCTTGCCGCCGAAGTGAGTGATACCCTGCTTCCAGGAAAAGCCACTAAGCTTCAGCTATTGACGACCGTACCGCAAACCGACACTGGTGCGCGAGATGGGTATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACACCGNTACTTCGGAAGAAGGTGTGCCTTTAGTAGGTGAACCCGTACAGGGGG]

[+] EMBL CD086867             [TCCCACTTCGTTTCCCACTTAGCCAATATTAGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGTGTCCGGACGTTAGCACCCGGTGCACTGTCTCCCAAGCTGTACTCATCGGTATTCGGAGTTTGCATAGGTTTGGTAAGTCGCCATGACCCCCTAGCCTAAACAGTGCTCTACCCCCGACGGTAATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATCTCCAGGTTAGCTCAGCCGGGTGTTGGATGTCCCGGTTCAAGCCTGTAGTCGTGCCTGGTAGGCAAATCCGCCGGGCTTAGATGAGGGGTGATAACGAGGCGGCTTGCCGCCGAAGTGAGTGATACCCTGCTTCCAGGAAAAGCCACTAAGCTTCAGCTATTGACGACCGTACCGCAAACCGACACTGGTGCGCGAGATGGGTATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACACCGNTACTTCGGAAGAAGGTGTGCCTTTAGTAGGTGAACCCGTACAGGGGG]




>consensus_10853#0
TCCCACTTCGTTTCCCACTTAGCCAATATTAGGGACCTTAGCTGGCGGTCTGGGTTGTTT
CCCTCTTGTGTCCGGACGTTAGCACCCGGTGCACTGTCTCCCAAGCTGTACTCATCGGTA
TTCGGAGTTTGCATAGGTTTGGTAAGTCGCCATGACCCCCTAGCCTAAACAGTGCTCTAC
CCCCGACGGTAATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATCTCC
AGGTTAGCTCAGCCGGGTGTTGGATGTCCCGGTTCAAGCCTGTAGTCGTGCCTGGTAGGC
AAATCCGCCGGGCTTAGATGAGGGGTGATAACGAGGCGGCTTGCCGCCGAAGTGAGTGAT
ACCCTGCTTCCAGGAAAAGCCACTAAGCTTCAGCTATTGACGACCGTACCGCAAACCGAC
ACTGGTGCGCGAGATGGGTATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGC
AAATTGACACCGNTACTTCGGAAGAAGGTGTGCCTTTAGTAGGTGAACCCGTACAGGGGG

consensusID : consensus_10853#1
NCBI blastX ! send the sequence to the NCBI site !
NCBI blastN ! send the sequence to the NCBI site !
Sequences nbr = 16
consensus length = 401
fasta sequence

                              [AAAACTCTTTGGACTTGTTGTTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAA-------T----CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC-GATATAACTGGATAAGCAGTAAAAGGACTAGACAAACATGAGATTGGTGACTATTCGTGAGTGAGTGGTGGGTCAGTGCCATGCTTATTC]

[+] EMBL CD155432             [AAAACTCTTTGGACTTGTTGTTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAG                                                                                                                                                     ]
[+] EMBL CD192035             [  tgatgtattGACTTGTTGTTGGTGTATTTGGTGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAAAATCGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAGTATATTGTGTGTGTGTATTGATGGGTATGGTGATCAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAG                                                                                                                                       ]
[+] EMBL CD117812             [                         GTATTTGGCGAAGTGGTGTGGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAAAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATATTGAACCTATATTTTGTGCTATTCGTCAGT                                                                                                                                                    ]
[+] EMBL CD184595             [                                                            GGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC GATATAACTGGATAAACAGTAAAAGGACTAGACAAACATGAGATTGGTGACTAT                                    ]
[+] EMBL CD155141             [                                                                               GTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTATAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC GATATAACTGGATAAGCAGTAAAAGGACTAGACAAACATGAGATTGGTGACTATTCGTGAGTGAGTGGTGGGTCAGTGCCATGCTTATTC]
[+] EMBL CD155290             [                                                                                  AACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTGAGTCGTCGTCAGCTAGGCGTACTTGTGATGTCG                                                                                                                      ]
[+] EMBL CD118154             [                                                                                     CGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC GATAT                                                                                     ]
[+] EMBL CD118102             [                                                                                     CGCCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTANAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGATATATTGAACCTATATTTTGTGCTATTCCTCAGTCGTCCTCATCA                                                                                                                                         ]
[+] EMBL CD118150             [                                                                                     CGTCGATATGTGGAGATGCAATCGATGGAATGGATCTGGCGATTTGTTGACCAGTTAAAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATT                                                                                                                                                           ]
[-] EMBL CD120019             [                                                                                                                                 TGTTGACGAGTTAGAATCGATGTAAATGAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC GATATAACTGGATAAGCAGTAAAAGGACTAGACAAACATGAGATTGGTGACTATTCGTGAGTGAGTGGTGGG                  ]
[+] EMBL CD155479             [                                                                                                                                                            GAA       T    CGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGAC                                                                                             ]
[+] EMBL CD113340             [                                                                                                                                                                           cagTGATATGTGGAGTTGCAGTATATTGTATGTGTGTCTTGATGGGTATGGCGGACAGGAGATAGTGAACCTATATTTTGCGCTATTCGTCAGTCGTCGTCAG                                                                                                                                           ]
[+] EMBL CD155323             [                                                                                                                                                                                                        ATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC GATATAACTGGATAAGCAGTAAAAGGACTAGAGAAA                                                      ]
[+] EMBL CD118044             [                                                                                                                                                                                                           TGTGTATTGATGGGTATGGTGGACAGGAAATACTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAG                                                                                                                    ]
[+] EMBL CD118135             [                                                                                                                                                                                                                                             GAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTGTTGGTGGACTC GATATAACTGGATAAGCAGTAAAAGGACTAGACAAACATGAGATTGATGACTATTCGTGAGTGAGTGGTGGGTCA               ]
[+] EMBL CD063916             [                                                                                                                                                                                                                                                                                                    gtGAGAGAAGTGGAGC TGTTGGTGGACTCAGATATAACTGGAT AGCAGTAAAAGGACTAGACAAACATGAGATTGGTGACTATTCGTGAGTG                           ]




>consensus_10853#1
AAAACTCTTTGGACTTGTTGTTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAA
GGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTT
TTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAATCGTTGATATGTGGAGTTGCA
GTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATTTTG
TGCTATTCGTCAGTCGTCGTCAGCAAGGCGTACTTGTGATGTCGAGAGAAGTGGAGCTTG
TTGGTGGACTCGATATAACTGGATAAGCAGTAAAAGGACTAGACAAACATGAGATTGGTG
ACTATTCGTGAGTGAGTGGTGGGTCAGTGCCATGCTTATTC

consensusID : consensus_10853#2
NCBI blastX ! send the sequence to the NCBI site !
NCBI blastN ! send the sequence to the NCBI site !
Sequences nbr = 4
consensus length = 284
fasta sequence

                              [TGACAACGACGCCTTGAATTGCCATGCCACCCACGGGTCTTTTCATGTATTGACTTGTTGTTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAATCGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATATTGTGCT]

[+] EMBL CD155361             [TGACAACGACGCCTTGAATTGCCATGCCACCCACGGGTCTTTTCATGTATTGACTTGTTGTTGGCGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCAATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGGTGTAAATGAATCGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGGTGGACAGGAGATAGTGAACCTATATATTGTGCT]
[+] EMBL CD155443             [TGACAACGTCGCCTTGAATTGCCATGCCACCCACGGGTCTTTTCATGTATTGACTTGTTGTTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAATCGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGATATGGTGGACAGGAGATcg                   ]
[+] EMBL CD155380             [TGACAACGACGCCTTGAATTGCCATGCCACCCACGGGTCTTTTCATGTATTGACTTGTTGTTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATGTAAATGAATCGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTc                                              ]
[+] EMBL CD155509             [                                                                                                                 GAATGAGTGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTCGTTGACGAGTTAGAATCGATGTAAATGAAg                                                                                    ]




>consensus_10853#2
TGACAACGACGCCTTGAATTGCCATGCCACCCACGGGTCTTTTCATGTATTGACTTGTTG
TTGGTGTATTTGGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAG
TGAACCGTCGATATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGT
TAGAATCGATGTAAATGAATCGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATT
GATGGGTATGGTGGACAGGAGATAGTGAACCTATATATTGTGCT

consensusID : consensus_10853#3
NCBI blastX ! send the sequence to the NCBI site !
NCBI blastN ! send the sequence to the NCBI site !
Sequences nbr = 1
consensus length = 136
fasta sequence

                              [AAACAGTTGCAGCCACCGATTCTCTGCGAACCCATTCGGCTCCAGGAGTAAATCCCTTCACCTAATAGAGGCACACCTTCTTCCGAAGTTACGGTGTCCATTTGCCGAGTTCCTTCTCCAGAGTTCTCTCAAAGCG]

[+] EMBL CD087772             [AAACAGTTGCAGCCACCGATTCTCTGCGAACCCATTCGGCTCCAGGAGTAAATCCCTTCACCTAATAGAGGCACACCTTCTTCCGAAGTTACGGTGTCCATTTGCCGAGTTCCTTCTCCAGAGTTCTCTCAAAGCG]




>consensus_10853#3
AAACAGTTGCAGCCACCGATTCTCTGCGAACCCATTCGGCTCCAGGAGTAAATCCCTTCA
CCTAATAGAGGCACACCTTCTTCCGAAGTTACGGTGTCCATTTGCCGAGTTCCTTCTCCA
GAGTTCTCTCAAAGCG

consensusID : consensus_10853#4
NCBI blastX ! send the sequence to the NCBI site !
NCBI blastN ! send the sequence to the NCBI site !
Sequences nbr = 1
consensus length = 89
fasta sequence

                              [AGAAGTGGAGCTTGTTGGTGGACTCGATATAACTGGATAAGCAGTAAAAGGACTANACAAACATGACATTAGAATCTTTCGTGAGTGAG]

[+] EMBL CD190864             [AGAAGTGGAGCTTGTTGGTGGACTCGATATAACTGGATAAGCAGTAAAAGGACTANACAAACATGACATTAGAATCTTTCGTGAGTGAG]




>consensus_10853#4
AGAAGTGGAGCTTGTTGGTGGACTCGATATAACTGGATAAGCAGTAAAAGGACTANACAA
ACATGACATTAGAATCTTTCGTGAGTGAG

consensus multiple alignement with clustalw

CLUSTAL W (1.82) multiple sequence alignment


consensus_10853#1      ------------------------------------------------------------
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      ------------------------------------------------------------
consensus_10853#3      ------------------------------------------------------------
consensus_10853#0      TCCCACTTCGTTTCCCACTTAGCCAATATTAGGGACCTTAGCTGGCGGTCTGGGTTGTTT 60
                                                                                   

consensus_10853#1      -----------------------------AAAACTCTTTGGACTTGTTGTTGGTGTATTT 31
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      ------------------------------------------------------------
consensus_10853#3      ------------------------------------------------------------
consensus_10853#0      CCCTCTTGTGTCCGGACGTTAGCACCCGGTGCACTGTCTCCCAAGCTGTACTCATCGGTA 120
                                                                                   

consensus_10853#1      GGCGAAGTGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCGA 91
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      ------------------------------------------------------------
consensus_10853#3      ------------------------------------------------------------
consensus_10853#0      TTCGGAGTTTGCATAGGTTTGGTAAGTCGCCATGACCCCCTAGCCTAAACAGTGCTCTAC 180
                                                                                   

consensus_10853#1      TATGTGGAGTTGCAATCGATGGAATGGTTTTGGTGATTTGTTGACGAGTTAGAATCGATG 151
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      ------------------------------------------TGACAACGACGCCTTGAA 18
consensus_10853#3      ------------------------------------------------------------
consensus_10853#0      CCCCGACGGTAATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATCTCC 240
                                                                                   

consensus_10853#1      TAAATGAATCGTTGATATGTGGAGTTGCAGTATATTGTATGTGTGTATTGATGGGTATGG 211
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      TTGCCATGCCACCCACGGGTCTTTTCATGTATTGACTTGTTGTTGGTGTATTTGGCGAAG 78
consensus_10853#3      ------------------------------------------------------------
consensus_10853#0      AGGTTAGCTCAGCCGGGTGTTGGATGTCCCGGTTCAAGCCTGTAGTCGTGCCTGGTAGGC 300
                                                                                   

consensus_10853#1      TGGACAGGAGATAGTGAACCTATATTTTGTGCTATTCGTCAGTCGTCGTCAG-CAAGGCG 270
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      TGGTGTTGTGCGCTATAGTTAAGGATGTAAATGAAGAATGAGTGAACCGTCG-ATATGTG 137
consensus_10853#3      ------AAACAGTTGCAGCCACCGAT-TCTCTGCGAACCCATTCGGCTCCAG-GAGTAAA 52
consensus_10853#0      AAATCCGCCGGGCTTAGATGAGGGGTGATAACGAGGCGGCTTGCCGCCGAAGTGAGTGAT 360
                                                                                   

consensus_10853#1      TACTTGTGATGTCGAGAGAAGT----GGAGC--TTGTTGGTG-----GACTCGATATAAC 319
consensus_10853#4      ----------------AGAAGT----GGAGC--TTGTTGGTG-----GACTCGATATAAC 33
consensus_10853#2      GAGTTGCAATCGATGGAATGGTTTTGGTGAT--TTGTTGACGAGTTAGAATCGATGTAAA 195
consensus_10853#3      TCCCTTCACCTAATAGAGGCAC----ACCTT--CTTCCGAAGTTACGGTGTCCATTTGCC 106
consensus_10853#0      ACCCTGCTTCCAGGAAAAGCCACTAAGCTTCAGCTATTGACGACCGTACCGCAAACCGAC 420
                                       *                 *   *  *         * *      

consensus_10853#1      TGGATAAGCAGTAAAAGGACTAGACAAACATGAGATTGGTGACTATTCGTGAGTGAGTGG 379
consensus_10853#4      TGGATAAGCAGTAAAAGGACTANACAAACATGACATTAGAATCT-TTCGTGAGTGAG--- 89
consensus_10853#2      TGAATCGTTGATATGTGGAGTTGCAGTATATTGTAT--GTGTGTATTGATGGGTATGGTG 253
consensus_10853#3      GAGTTCCTTCTCCAGAGTTCTCTCAAAGCG------------------------------ 136
consensus_10853#0      ACTGGTGCGCGAGATGGGTATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGC 480
                                       *   *                                       

consensus_10853#1      TGGGTCAGTGCCATGCTTATTC-------------------------------------- 401
consensus_10853#4      ------------------------------------------------------------
consensus_10853#2      GACAGGAGATAGTGAACCTATATATTGTGCT----------------------------- 284
consensus_10853#3      ------------------------------------------------------------
consensus_10853#0      AAATTGACACCGNTACTTCGGAAGAAGGTGTGCCTTTAGTAGGTGAACCCGTACAGGGGG 540