Rest and Trans Map

Rest and Trans Map accepts a DNA sequence and returns a textual map showing the positions of restriction endonuclease cut sites. The translation of the DNA sequence is also given, in the reading frame you specify. You can choose the number of bases per line of the map, and whether to show the DNA in its single-stranded or double-stranded form. Use the output of this program as a reference when planning cloning strategies, particularly when you need to consider the reading frames of the molecules you are ligating.

Paste the raw or FASTA sequence into the text area below.

  • The sequence is
  • Show bases per line.
  • Show the translation for
  • Show the DNA sequence as DNA.

Enter the restriction site expressions. The default set of expressions describes commonly-used restriction sites. Slashes mark the boundary of the pattern to match, while the round brackets surround the name of the site. The number located after the closing round bracket is the distance of the cleavage site from the 3' end of the recognition sequence. Within the pattern square brackets surround possible bases present at a single position. Each expression is followed by a comma, except for the last expression. Enzymes that recognize non-palindromic sites are given two entries, one for each strand.

Enter the genetic code expressions. The default set of expressions describes the standard genetic code. Slashes mark the boundary of the pattern to match, while the equal sign and letter specify the amino acid described by the pattern. Within the pattern square brackets surround possible bases present at a single position. The vertical bar separates two distinct patterns that represent the same amino acid. Each expression is followed by a comma, except for the last expression.

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