[BiO BB] remove CTL-M and Buying a bioinformatics workstation
Tristan Fiedler
tfiedler at rsmas.miami.edu
Wed Sep 3 15:09:34 EDT 2003
Dear Bio Gurus!
Two quick questions :
1. could someone please assist me in writing a shell script (awk, sed,
etc.) which would use a loop to run thru about 1000 files (filenames all
end in '.seq') and remove all occurences of control-M, resulting in a file
containing the sequence on a single line.
Currently each file looks similar to :
% cat -v seq_018_G05.seq
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA^M
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGG^M
TTTTTTTTTTTTTTTTCCCAAAAAAAAAAAAA^M
2. We are planning to buy a workstation for our local (~3 labs producing
sequences from an ABI sequencer) genomics needs (lots of blast runs,
database management, standard bioinformatics software), and were planning
on getting something like :
4 GB RAM (is this enough for doing local blast searches against genbank?)
2 x 3 GHz Xeon processors (how about Mac OSX?)
400 GB storage
Thank you - and feel free to reply directly to me (not waste bb resources).
Cheers!
--
Tristan J. Fiedler, Ph.D.
Postdoctoral Research Fellow
NIEHS Marine & Freshwater Biomedical Sciences Center
Rosenstiel School of Marine & Atmospheric Sciences
University of Miami
tfiedler at rsmas.miami.edu
t.fiedler at umiami.edu (alias)
305-361-4626
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