[BiO BB] Testing blast, getting "Unable to open BLOSUM62" error?
Theodore H. Smith
delete at elfdata.com
Thu Feb 16 16:43:10 EST 2006
Hi people,
I've installed blast on my computer. I'm getting some errors using a
custom DB.
ShatterProof% cd /Users/theodore/Desktop/customgene.faa/
ShatterProof% blastall -p blastp -d customgene.txt -i query.txt -o
result.out
[NULL_Caption] WARNING: [000.000] query: Unable to open BLOSUM62
[NULL_Caption] WARNING: [000.000] query: BlastScoreBlkMatFill
returned non-zero status
[NULL_Caption] WARNING: [000.000] query: SetUpBlastSearch failed.
however, this example copied from ncbi's website, works just fine:
ShatterProof% cd /usr/blast/dbs/
ShatterProof% blastall -p blastn -d ecoli.nt -i query.txt -o test.out
I get a text file containing the result.
Is that because I'm using blastp in the first attempt, and blastn in
the second?
Where does BLAST expect the BLOSUM62 file to be?
--
http://elfdata.com/plugin/
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