[BiO BB] Testing blast, getting "Unable to open BLOSUM62" error?
Pamela Culpepper
pculpep at hotmail.com
Thu Feb 16 16:53:36 EST 2006
The is an environment variable -- BLASTMAT -- that you can set to the
location of your matrix file.
Otherwise, the data/ directory should contain the filters.
Pam
>From: "Theodore H. Smith" <delete at elfdata.com>
>Reply-To: "The general forum at Bioinformatics.Org"
><bio_bulletin_board at bioinformatics.org>
>To: "The general forum at Bioinformatics.Org"
><bio_bulletin_board at bioinformatics.org>
>Subject: [BiO BB] Testing blast, getting "Unable to open BLOSUM62" error?
>Date: Thu, 16 Feb 2006 21:43:10 +0000
>
>Hi people,
>
>I've installed blast on my computer. I'm getting some errors using a
>custom DB.
>
>ShatterProof% cd /Users/theodore/Desktop/customgene.faa/
>ShatterProof% blastall -p blastp -d customgene.txt -i query.txt -o
>result.out
>
>[NULL_Caption] WARNING: [000.000] query: Unable to open BLOSUM62
>[NULL_Caption] WARNING: [000.000] query: BlastScoreBlkMatFill returned
>non-zero status
>[NULL_Caption] WARNING: [000.000] query: SetUpBlastSearch failed.
>
>
>however, this example copied from ncbi's website, works just fine:
>
>ShatterProof% cd /usr/blast/dbs/
>ShatterProof% blastall -p blastn -d ecoli.nt -i query.txt -o test.out
>
>I get a text file containing the result.
>
>Is that because I'm using blastp in the first attempt, and blastn in the
>second?
>
>Where does BLAST expect the BLOSUM62 file to be?
>
>--
>http://elfdata.com/plugin/
>
>
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